LOCUS       DATGSI010000001         1721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_141969, whole genome shotgun sequence.
ACCESSION   DATGSI010000001 DATGSI010000000
VERSION     DATGSI010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141969"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..>1721
                     /locus_tag="VK031_00005"
     CDS             54..>1721
                     /locus_tag="VK031_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HLS52329.1"
                     /translation="MKIYIFDLEANGFLDQATKVHCGVFKELDSDSIYRFTPEEIHDM
                     LSFMEECDVLIGHNCIGYDFPLLKKLYGFEYKGRKVDTMVMSRLLDPRRQSPPHCPNK
                     RAPHSVEAWGYRVGRGKPEHDDWENYSPEMLHRCEEDTLIQELIYKELLKEAEGYNWR
                     DAFLLTFRLFENLQKQEEYGWKVDKEYMEKCIDTLDRWVRRIDNAIQDKLPIIIEINE
                     TKAKDGTYNYVRKPFLKSGKYSKSVKDWYGNNNICIDSVIVVGPFSRITTRRADLDKR
                     TEIIPFLLDQGWKPLEYNYSSTTGERTSPKLSKDDPFDGIEGRLGKLVVKRTQCKHRR
                     SSIEGLLGLIREDGAIPSVINNLAVTGRATHRNIVNIPRSGSFFGKQMRKIFSSREGM
                     VLVSTDSAGNQLRQLAARMEDDDYIKALVEGKESEGTDIHTLNQKRAGLPDRDTAKTF
                     IYGFLFGAGNQKIGSIISGTAEDGRRLKEEFIRQLPALDGLLGKISKEWKETAKKRFN
                     SKFNRMEHYDGIITGLDGRPIKVPSEHMLLVYLLQSDEAIHMAAAYNI"
BASE COUNT          610 a          263 c          389 g          459 t
ORIGIN      
        1 ctatcgtatt gtagaaaggt ttaagaaaga attgaaagaa tacttcagtt gaaatgaaaa
       61 tttatatatt tgatttagag gctaatggtt tcctggatca agccactaaa gttcattgcg
      121 gagtgttcaa agagctagat tctgacagta tatataggtt cacccctgaa gaaatccatg
      181 acatgttatc cttcatggag gaatgcgatg ttctaatagg acataattgc ataggatatg
      241 attttccttt attaaagaag ttatatgggt tcgagtataa aggaagaaaa gtagacacca
      301 tggttatgtc taggctttta gatcctagaa gacaatctcc tccacattgc cctaacaaaa
      361 gagcccctca ctctgttgaa gcctgggggt atagagtagg tagggggaag ccagaacacg
      421 acgattggga aaactactct ccagagatgc ttcatagatg cgaagaagac actttaattc
      481 aagagctaat atataaagag ttattaaaag aagctgaagg atataattgg agagatgctt
      541 tcctactcac tttccgttta tttgaaaacc ttcaaaagca agaagaatat gggtggaaag
      601 tagataaaga gtatatggaa aagtgtatcg atactttaga tagatgggtt aggaggattg
      661 ataacgctat ccaagataaa ctaccaataa taatagaaat aaatgaaaca aaagcaaaag
      721 acggaactta caactacgtt cggaagccgt tccttaagtc tggaaaatat agtaaaagcg
      781 ttaaagactg gtatggaaac aataatatat gtatcgatag cgttattgtt gttggcccct
      841 tttctaggat tactactagg cgcgctgact tagacaagag gacagagatt ataccgttct
      901 tattagatca aggatggaag cctttagaat ataattatag ttctaccacc ggagaaagaa
      961 ctagccctaa actgtctaaa gatgatcctt tcgacggtat tgagggaagg ctcggaaagc
     1021 ttgtggttaa aagaacacaa tgtaaacaca gaaggagctc tatagaggga ttgttaggat
     1081 tgataagaga ggacggagca ataccatctg ttattaacaa tctagctgtt acagggagag
     1141 ccacccacag aaatattgtt aatatcccta ggagcggtag cttttttggt aagcaaatga
     1201 gaaaaatatt ctctagtagg gaaggaatgg ttttagtttc tactgattct gccggaaatc
     1261 agcttagaca gctagcagct agaatggaag atgacgacta tattaaagct ttagttgaag
     1321 gtaaagaatc agaaggcaca gacatccaca ccttaaacca aaaaagagca ggactccctg
     1381 atagggacac tgctaagacg tttatatatg ggttcttatt cggagcagga aaccaaaaga
     1441 taggatctat tatatctggg actgccgaag atgggagaag acttaaagaa gaatttatca
     1501 ggcagcttcc tgcactggac ggacttttag ggaagatatc taaagagtgg aaggagacag
     1561 ctaagaaaag gtttaattca aaatttaata ggatggagca ttacgatgga attataacag
     1621 gattggatgg aagacctata aaggttccct ccgaacatat gttattggtt tatttattac
     1681 aatcagatga ggccatccat atggcagcag cttataatat a
//
LOCUS       DATGSI010000002         1065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_47507, whole genome shotgun sequence.
ACCESSION   DATGSI010000002 DATGSI010000000
VERSION     DATGSI010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47507"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..>1065)
                     /locus_tag="VK031_00010"
     CDS             complement(26..>1065)
                     /locus_tag="VK031_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLS52330.1"
                     /translation="GMIQQYFQGEERGRAFGYFGTTVGVSLGVGPVLGGLLINFAGTD
                     LGWRLTFLMNVPIGMLTIILGLMWFPRPLIKLLRVTSYYATLRSLDPIGALIFTLGVL
                     CVLLPFMGNQSLFILLLPSSFLLFQLWVKWEKYYSSLGYEPMVDLNIFRSSNYTNGVL
                     IMSLYFLGKTSIWLLIALYMQQGVGKSAFETSLVGIPGALLTAYASNWAGKRVTYFGR
                     KIVVAGLSFTIFSLLLSIGFILLHELYQFSIWWLLFSLAFFGLGQGATTSPNQAITLA
                     DVPVAYAGSSGAIMQTAQRVGASVGIAVITAITFTTVNYFSWTVAIIMGFITIILIIT
                     LALLVAVKDLK"
BASE COUNT          388 a          226 c          190 g          261 t
ORIGIN      
        1 gtgaaactag taatggctgt agacatcact ttaaatcttt aacagccacg agtagtgcca
       61 aggtgataat caagattata gtaataaaac ccataataat cgcaactgtc catgaaaaat
      121 aatttacagt ggtaaaagta atagcagtaa taacagcaat acctaccgag gcaccaacac
      181 gttgtgctgt ttgcataatg gcaccagagc tgcctgcata ggccacagga acatcagcta
      241 gagtaatagc ttgatttggg ctagtagtag ccccttggcc taatccgaag aaagccaagg
      301 aaaataataa ccaccatata ctgaattgat aaagctcatg cagtaaaata aatcctatgc
      361 ttaaaagcaa actaaaaata gtaaaagata agcctgcaac tactatttta cgcccaaagt
      421 aggtaacacg ttttccagcc caatttgagg cataagccgt tagtaaggct cctgggatac
      481 caactaagct agtttcaaag gctgatttcc ccaccccttg ttgcatatat agagcgatga
      541 gtaaccaaat gctcgtcttg cccaagaaat agagactcat tattaaaacc ccattagtgt
      601 agtttgaact acgaaaaatg tttaaatcta ccataggctc ataacctaga gagctatagt
      661 atttttccca ctttacccag agctgaaaca ataaaaaact agacggtaat aataatataa
      721 atagggattg attccccata aaaggaagca acacacagag gacacctaag gtaaaaatta
      781 gtgcgcctat agggtctaat gagcgtaaag tagcataata tgaagtgacc ctcaataatt
      841 taattagagg tcgtggaaac cacattaatc ctaaaattat tgttagcatc cctataggaa
      901 cattcattag aaaagttaag cgccaaccta gatctgtacc ggcaaaattt attagcaatc
      961 cacctaagac cggccctact cccaatgaaa caccaacagt ggtaccaaag taaccaaaag
     1021 ctctgccgcg ttcttcgccc tggaagtact gttgaatcat tccca
//
LOCUS       DATGSI010000003         4518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_142038, whole genome shotgun sequence.
ACCESSION   DATGSI010000003 DATGSI010000000
VERSION     DATGSI010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142038"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VK031_00015"
     CDS             complement(<1..549)
                     /locus_tag="VK031_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HLS52331.1"
                     /translation="MYIKNNRKKIIFLLIIFVLSLNYMQVYGQKIDNLKNEQKNIKQQ
                     IKDAQDEIKTIEQQSRDVSKQISDLDAEMNNATIELDKVEREIDKIQRNIDKNARELE
                     EAEKNLQERQELFNSRLRVMYMNGNVGYLELLLTSTSIKDFLSRREMIQSIAAHDTEL
                     INYMKEQRETIQEKKKELEGQRA"
     gene            complement(561..1460)
                     /gene="ftsX"
                     /locus_tag="VK031_00020"
     CDS             complement(561..1460)
                     /gene="ftsX"
                     /locus_tag="VK031_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HLS52332.1"
                     /translation="MKIRQIKNMTKQGIQGVWRNRGMGLASISSITAVLMILGVVLIL
                     ILSINSLVADTQTKFDEIEIYLVDDIRDEDMNKIENILSNKRGVASVIFRSRDEALRI
                     MKEDWGEEAYLLKGLEEDNTLPDSYIVELEDIAYSHEVVESLSSIETIEKINYHKEII
                     DKLKIFANYIRIGGIIIISVLIFVSIFIISNTIKLTVTSRSREISIMKYVGATNGYIK
                     GPFIIEGVVFGLLGALLSIGIVYYSYEYFFNSVNEKIYTFFTIVLIAPSAILKDISII
                     FIAIGVGIGALGSLVSMKRFLNV"
     gene            complement(1450..2136)
                     /gene="ftsE"
                     /locus_tag="VK031_00025"
     CDS             complement(1450..2136)
                     /gene="ftsE"
                     /locus_tag="VK031_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HLS52333.1"
                     /translation="MIKLVNINKEYKNGVKALSDINLDIKKGEFVFLVGSSGAGKSTI
                     VKLLLKEEEPTSGKIYLNNMDITKVSRRRIHIVRRNLGVVFQDFRLLPNKTVYENVAF
                     AMEILGIPVREIRRRVPLVLSMVDLSRKAYSFPDQLSGGEHQRVAIARAIVNNPPILL
                     ADEPTGNLDPDTAWEIMKVLNDINNRGTTVIMATHAKEIVNHMRKRVVTIDKGYVISD
                     EEKGSYTYED"
     gene            2251..2817
                     /locus_tag="VK031_00030"
     CDS             2251..2817
                     /locus_tag="VK031_00030"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HLS52334.1"
                     /translation="MRKKLLRREMLDKLSKMDEKKHKKYSQIIVNRFINSSFYKEAKT
                     IMSFVSFGHEINTHDLIKNSLLQGKKILVPITIPEKRELKVSQLKDFDELELGHYNIL
                     TPKDEYIRLRDPLDIDLIIVPGLAFDKKGFRVGYGGGYYDRFLAQLPQVTKISLAFHM
                     QTLPQLPRENFDIPVDYIYTERGIIECK"
     gene            2899..4083
                     /locus_tag="VK031_00035"
     CDS             2899..4083
                     /locus_tag="VK031_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005953873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HLS52335.1"
                     /translation="MGRTVATTVRGIRTPIVKEGDDLVSIVVDSVLKAAKGEGFQLRD
                     KDVIGITESLVARAQGNYATVDHIAQDIDGKFKDEIGVVFPILSRNRFSVLLKGIAKS
                     GKNIHLLLSYPADEVGNHLMDIDKMEELGINPYSDTLTEEDYRRLFGDKVTHPFTGID
                     YVDLYKKMGNGNINIYFSNDPRTILNYTKDILIANVHDRKRTRRILEKAGANSLWGLE
                     DILTSSIDGSGYNPEYGLLGANLASEKEVKLFPRDSQYYVEEIQKVLKEKTGKKIEVM
                     VYGDGAFKDPVGKIWELADPVVAPGFTDGLVGTPSEIKLKYIVDTKLDQLDREEISSL
                     VKQKIYSKGVDLLDQAESLGTTPRQITDLLGSLCDLTSGSGDKGTPVVLVQGYFDNFA
                     SE"
     gene            complement(4121..>4518)
                     /locus_tag="VK031_00040"
     CDS             complement(4121..>4518)
                     /locus_tag="VK031_00040"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HLS52336.1"
                     /translation="IGSLIVPKILKGKEDTVRYKVVEEEDIPEQIVDMLPKYIMEERA
                     LTCKHKDDIYVIVTRGEKKSKGFSVDIDKIVREKHSKDKFQITVYAKFEDPKPDEVLP
                     QEYDYPFIIVKTRLKQMPEQVNLEIEYID"
BASE COUNT         1397 a          820 c          743 g         1558 t
ORIGIN      
        1 agccctttgt ccttccaatt ccttcttttt ctcctggatg gtttcccttt gctccttcat
       61 ataattgatt aattctgtat catgggcagc aatagattgg atcatttccc ttcttgacag
      121 aaaatcctta atgctggtag aggttaataa tagttctaag tagcccacat ttccattcat
      181 atacataact ctcaatctag agttaaagag ttcctgcctt tcttgaagat ttttttctgc
      241 ctcttctagc tccctggcat ttttatctat attcctttgt attttatcaa tctctctttc
      301 caccttgtct aattctatag tggcattatt catctcagca tctaaatcac ttatctgttt
      361 tgacacatcc ctagactgtt gctctatagt ctttatttca tcctgagcat ctttaatttg
      421 ttgcttaata tttttctgct cattttttaa attgtctatc ttctgtccat agacttgcat
      481 atagtttagg cttagaacaa atattattag tagaaatata atcttttttc tattgttttt
      541 tatatacatt tatgctcccc ctatacatta agaaatcttt tcatggaaac cagactacct
      601 agagccccta ttcctactcc aattgcaata aaaattattg atatatcttt taaaatagct
      661 gaaggtgcta taagtacaat ggtgaaaaag gtatatattt tttcgtttac tgaattaaaa
      721 aagtactcat aggaataata tactatccct attgataaca aggcacctag cagcccaaat
      781 actactccct ctatgataaa tggtcccttt atataaccat tggtagctcc cacgtatttc
      841 ataatgctaa tttccctact tctagatgta acagtaagtt taattgtgtt ggagataata
      901 aaaatagaca caaatattaa aaccgatatg attattatgc cacctatcct gatatagttg
      961 gcaaatattt ttaatttatc tataatctcc ttgtgatagt taatcttttc tatggtttct
     1021 atagagctta gggattctac cacctcatgg gaatatgcaa tatcctccag ctcaactata
     1081 tatgaatctg gaagggtatt atcctcctcc aatcccttta gaagataggc ttcttcaccc
     1141 cagtcttctt tcataattct aagggcctca tccctagatc taaagattac agaggcaacc
     1201 cctcttttat tacttagaat attttctatt ttattcatat cttcatctct aatatcatct
     1261 accaggtaga tttcaatttc atcaaacttg gtttgggtat cagcaaccag agaattgatg
     1321 ctaagaataa gtattaaaac cacacctaaa atcattaaaa ctgctgtaat tgaggaaatt
     1381 gatgccagtc ccatccctct atttctccaa acaccctgta taccttgctt ggtcatgttt
     1441 tttatttgcc taatcttcat aggtataact tcccttctcc tcatcgctga taacataacc
     1501 tttgtcaata gttactaccc tttttctcat atgattgact atctctttag catgggtagc
     1561 cataataact gttgttcccc tattattaat atcatttagg actttcataa tctcccaagc
     1621 cgtatctggg tcaagatttc cagtaggctc gtccgccaac aaaataggcg gattgttaac
     1681 tattgccctg gctatggcaa ctctttgatg ctctcctcct gataattgat caggaaaact
     1741 ataagccttg cggcttaaat caaccatact taatactaag ggtacccttc ttctgatttc
     1801 tctgacaggt atacccagta tttccatagc aaaagccaca ttttcataaa ctgttttgtt
     1861 aggcagcagt ctaaaatctt gaaatactac tcccaaattt ctcctaacta tatgtatcct
     1921 tctcctgctt accttggtta tatccatatt gtttaaatat attttaccac tggttggctc
     1981 ttcttccttt aatagtaatt ttactatagt agatttacca gctccgctgg agcctaccaa
     2041 aaaaacaaat tctccttttt ttatatctaa atttatatca gataaagctt tgacgccatt
     2101 tttgtactcc ttatttatgt taactagttt tatcaaattg tacacctcaa tatttacttc
     2161 tcttaaatag aagattaaag ttttaaaatc atattcttag tatattataa tatagattga
     2221 cctataaata aactgtttga ggtgatttat ttgagaaaaa aattacttag aagggaaatg
     2281 ttggataaac tgtcgaagat ggatgaaaaa aaacataaaa aatatagcca aatcattgta
     2341 aataggttta ttaactcctc cttttataaa gaagctaaga ctattatgtc atttgtaagc
     2401 tttggccacg aaattaacac ccatgacctt attaaaaata gtcttttaca gggaaagaag
     2461 attttggttc ccataaccat cccagaaaaa agagagctta aagtttctca gctaaaagat
     2521 ttcgatgaat tggaactggg gcattacaat attttaactc ccaaggatga atatattcgt
     2581 cttagggatc ctctggatat agatttaatt attgtgcctg ggttggcctt tgataaaaaa
     2641 ggttttaggg taggctatgg cggtggatac tacgatagat tcttagccca attgcctcaa
     2701 gttacaaaaa tatccctagc cttccatatg caaactcttc cacaactacc aagggagaat
     2761 tttgacattc cagtggacta tatatacact gaaaggggga ttatagaatg taaatagttt
     2821 tcttttctac tggtaattta aaacaatatg atataatata gtagatgctt tgattttttg
     2881 tataggaggg gcaggtaaat gggtagaact gttgcaacta ctgtaagggg aataagaaca
     2941 cctatagtca aggaagggga tgacctggtc agtattgttg tagattcagt tttaaaggcg
     3001 gcaaaaggcg agggttttca actgagagac aaggacgtta ttggtataac agaatctcta
     3061 gtagcaaggg cccagggaaa ttatgcaact gtagatcata ttgcccagga tatagatgga
     3121 aaatttaaag atgagattgg agttgttttc cccatattaa gtcgtaatag gttttctgtc
     3181 ttgttaaaag ggattgctaa aagtggaaaa aatattcacc tcctattatc atatcctgcc
     3241 gatgaagtag gtaatcatct gatggatata gataagatgg aagagttagg cattaatcca
     3301 tattcagata cccttactga agaggattat agaaggctat ttggtgataa ggtaacccac
     3361 ccctttaccg gtatagatta tgttgattta tacaaaaaaa tgggaaatgg taatataaat
     3421 atatatttct caaatgatcc taggacaatt ttaaactata ctaaggatat acttatagcc
     3481 aatgtgcatg ataggaaaag aactagacga attctagaaa aagctggggc caattcccta
     3541 tggggattag aggatatatt aacctcctcc atagatggta gtggatataa tccagagtat
     3601 ggtctacttg gggctaattt ggcttctgaa aaggaagtaa aattgtttcc tagagacagc
     3661 caatattatg tggaagaaat ccagaaagta cttaaggaaa agacaggaaa aaaaatagaa
     3721 gttatggttt atggagatgg agcctttaag gatccagtgg gcaagatttg ggagttagca
     3781 gatccagtgg tagcaccagg ctttactgat gggctagtag gaacacctag tgagattaag
     3841 ctaaaatata tagtagacac taaactagac cagcttgata gggaagaaat ttcaagctta
     3901 gtaaagcaaa aaatctatag caagggtgta gatttattgg atcaggctga atccttaggt
     3961 actactccta ggcaaattac agatctgtta ggaagccttt gtgatttaac cagtggcagt
     4021 ggagataagg gaacccctgt tgttttagtc caaggctatt ttgataactt tgcatctgaa
     4081 taatggtaaa aaggaagcag gcctgcttcc tttttttata ttagtctatg tactctattt
     4141 ctagattaac ttgttctggc atctgtttta gcctagtttt tactattata aagggataat
     4201 catactcttg aggtaaaacc tcatctggct ttggatcctc aaacttagca taaacagtaa
     4261 tctggaattt atcctttgaa tgtttttctc ttactatttt gtctatgtct actgaaaaac
     4321 ctttagactt tttctcccct ctagttacta ttacatatat atcatcctta tgcttacagg
     4381 taagagccct ttcctccatg atatatttag gcaacatatc cactatttgc tccggtatat
     4441 cctcttcctc gactacctta tatcttacag tatcttcttt gcctttaagt attttaggaa
     4501 ctataaggct tcctatta
//
LOCUS       DATGSI010000004         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_236522, whole genome shotgun sequence.
ACCESSION   DATGSI010000004 DATGSI010000000
VERSION     DATGSI010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236522"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..337)
                     /locus_tag="VK031_00045"
                     /pseudo
     CDS             complement(26..337)
                     /locus_tag="VK031_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
     gene            complement(571..1080)
                     /locus_tag="VK031_00050"
     CDS             complement(571..1080)
                     /locus_tag="VK031_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLS52337.1"
                     /translation="MDIMEAILTRRSIREFTGQVIEEDKLKKLLQAGFQAPSAHNKEP
                     REFVVVKDRKKIKEITDFHEYAQMLPDAGCGIIVCGDSSKQDLEGFLIADCSAAIQNI
                     LLAAHGLGLGAVWCGLYPRKALMANMGRVLNLPENIMPVGLVAVGYKAEEKEGIDRYN
                     QDCIHLNSW"
     gene            complement(1149..1475)
                     /locus_tag="VK031_00055"
     CDS             complement(1149..1475)
                     /locus_tag="VK031_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HLS52338.1"
                     /translation="MLKLKSREDITDKIDQGGMVLLYFGSKNCGVCVDLKPKIQVMLK
                     DYPEIEAYHVDIERSPNLAINYNIFTIPGILLFIEGKENIREARHISVRDLERKINRY
                     YKLFFG"
     gene            1856..>2097
                     /locus_tag="VK031_00060"
     CDS             1856..>2097
                     /locus_tag="VK031_00060"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HLS52339.1"
                     /translation="MAVVDWKEVIEFWFDPEHIPLHFVEDKEFDEKIRKRFLATWEAA
                     AEGLLVHWRNDIRGRLAEIIVLDQFSRNLWRNDIRT"
BASE COUNT          636 a          425 c          270 g          766 t
ORIGIN      
        1 tttcgctcct tttataattt ataacttagg taagcttctc ctcccactcc tttttcttaa
       61 atcctaccaa aacagtatct ttacctatta aaagaggcct cttaatcaac atgccatcag
      121 tggccaaaat tttaatcgcc tcatcttggc tcatttccgg tagcttttcc tttaagttta
      181 attccctgta tacctttccc ctagcattaa aaaagtcctt taaaggataa tcgctcctat
      241 ttatccactc ttttaattct tcctccctag ggttttcact ttttataggt ctttccttgt
      301 aatctattcc cttatcatct agccaggccc tggcctttct acaggtggtt caactggata
      361 agcatataaa caaatactcc ataatcagat cctcctttgt atatcagtta aaaaatttat
      421 acccattttt tattttgttt attattcttt attaatcttt ggttaattat aacatagtat
      481 atgcccctaa agatatgaat tgtacaacat tttgtaaatt aaacccataa aaatcaagtc
      541 ctccataatg gagggcttga cctaaatata ttaccaacta tttaaatgta tacagtcttg
      601 attatatctg tcaatacctt ccttctcttc agccttgtat cctactgcca ccagcccaac
      661 tggcattata ttttctggta aatttaatac ccggcccata tttgccatta aagcttttct
      721 aggataaaga ccacaccaaa ctgctcccag ccctaaacca tgggcagcca atagtatatt
      781 ttgaatagct gcagaacaat ctgctattaa gaatccttcc aaatcttgtt tagagctatc
      841 cccacaaact ataatgccac aaccagcatc tggtagcatt tgggcatact catgaaaatc
      901 tgtaatctcc tttattttct tcctgtcttt tactactaca aattctcttg gttccttatt
      961 atgggcagat ggagcttgaa atcctgcctg taatagtttt ttcaacttat cctcctctat
     1021 tacctgccca gtaaactccc taatacttct cctagttaaa atggcttcca ttatatccat
     1081 atatcttcct ccctatttat taaatatttt tatctatcct ttcatggaaa gatttattaa
     1141 acctgatgtc aaccaaagaa aagtttgtaa tatctattga ttttcctttc taaatccctg
     1201 acactgatgt gcctggcttc tctgatattt tcttttcctt ctataaaaag taaaatacca
     1261 ggtatagtaa aaatattata attgatagcc agattaggtg acctttctat atctacatga
     1321 taagcttcta tttcaggata atcctttagc attacctgaa ttttaggctt taaatctaca
     1381 caaacaccac aatttttact accaaagtat aataaaacca ttccaccttg atctatctta
     1441 tctgttatat cctcccttga ctttagcttt aacaagcctc cacctccgtc tcctttatag
     1501 gttgcctaaa tttctaggaa ctactttatt tataatcata ctgttattat aacataattc
     1561 acctattaaa tttaaagatt gagggatcct ttcccaatgg gaaaatatat atttaggata
     1621 tatatttatt gtcatatcct aatataaaga caattttata gatctaatta taagaattac
     1681 cagattaata gataggtttt gattaaaggt ttaactttta atacttacta tatttatttg
     1741 aaccaactac atttccaaat ttttcctccc ctttccctga gtaatgaaaa attatactat
     1801 aaatctttct tttgggtata tggtaaataa tagataaaat taaaggagtg attatatggc
     1861 tgttgtagac tggaaggaag taattgaatt ttggtttgat cctgagcata tccctcttca
     1921 ctttgttgaa gataaggaat ttgatgaaaa aatcagaaaa agattcctag ccacctggga
     1981 ggctgcagca gaggggcttt tagttcattg gagaaatgat atcaggggga gattggctga
     2041 aatcatagtt ttggatcaat tctctagaaa cctttggaga aatgatattc ggactta
//
LOCUS       DATGSI010000005         1036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_47929, whole genome shotgun sequence.
ACCESSION   DATGSI010000005 DATGSI010000000
VERSION     DATGSI010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47929"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..441
                     /locus_tag="VK031_00065"
     CDS             163..441
                     /locus_tag="VK031_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52340.1"
                     /translation="MRKQIQKIVESIFSLSLIIAILGGGVIFCMFIIGIIIGGSTGES
                     LAVSASKVVMPYFIRSAAIAVLCGLISFYTTGKHTLSLDEEEKELAEN"
     gene            686..>1036
                     /locus_tag="VK031_00070"
     CDS             686..>1036
                     /locus_tag="VK031_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52341.1"
                     /translation="MALKRSKTLDLNLLSETINLMNKADDFQVISEIIFNFIENFVSY
                     NMAVIYKLNEKDKILEIVSCIGSDTEKMKKRMPFRVGESAVGLVAKDKKALLINDVLN
                     SKEITVRQYYDEDPL"
BASE COUNT          359 a          135 c          194 g          348 t
ORIGIN      
        1 ccgccattgg ccttggccat gtatgcggct atgggaattg ctgaatcgaa gatgaaagaa
       61 acctctattt tagcctttat ttgggtcttt acacacttag ccgttgtcat aattatgctg
      121 gctggtataa taccggttcc atttatataa ggaggggaga caatgaggaa acagatacaa
      181 aaaatagtag agtccatatt tagtctttct cttataatcg ccatactagg tggaggagta
      241 attttctgta tgtttattat aggaattata ataggtggct caacagggga atccttagca
      301 gtaagtgcct ctaaggttgt aatgccttac tttatcagat ctgcagctat agcagtctta
      361 tgtggattga tatccttcta tactacaggt aagcatacat tatccttaga tgaagaagag
      421 aaagaattag cagaaaatta atataaaatt atagggataa gctgttattt gggcttatcc
      481 ttatttttat attaatttct aaataatatt ccttttaatg tacaatggta ttatttttgc
      541 atattaatat attggtacta tatttttgac aactgccaat ataatgaatt acaaaaatat
      601 taaacttgta tattctcctt attatattgc ttcatagaaa attctaatat aaaatggtgt
      661 tagaagtcac taaggaggag agggtatggc attaaaaaga tcaaagactt tagacttaaa
      721 tctgctgtcg gaaactataa atttaatgaa taaggcagat gattttcagg ttatttccga
      781 aataatattt aactttattg aaaactttgt atcctataat atggcagtca tctacaagtt
      841 aaatgaaaag gataagattc ttgaaatcgt ttcctgtata ggttcagaca ctgaaaaaat
      901 gaaaaaaaga atgcccttta gagtagggga aagtgcagta ggactagttg ctaaagataa
      961 aaaggcattg ttgattaatg atgttttaaa ttctaaggaa ataaccgtaa gacaatatta
     1021 tgatgaggat ccgtta
//
LOCUS       DATGSI010000006         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_567541, whole genome shotgun sequence.
ACCESSION   DATGSI010000006 DATGSI010000000
VERSION     DATGSI010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567541"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VK031_00075"
     CDS             <1..431
                     /locus_tag="VK031_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HLS52342.1"
                     /translation="ADTIIGNCDSMIFLGGKEKTTLKDLSETLGKETIDMFNTSDTRG
                     TQRSYGLNYQKLGKELMSMDELAVMDGGKCILQLRGVRPFLSDKYDITKHPNYKYLSD
                     FDKRNTFNIEKYLSTKLKVKSKEVYNVYEIDLADENKTAD"
     gene            479..694
                     /locus_tag="VK031_00080"
     CDS             479..694
                     /locus_tag="VK031_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HLS52343.1"
                     /translation="MAFFNSAVDVLQTLVIALGAGLGIWGVINLLEGYGNDNPGAKSQ
                     GMKQLMAGGGVALIGMTLVPLLSGLFG"
     gene            706..1575
                     /locus_tag="VK031_00085"
     CDS             706..1575
                     /locus_tag="VK031_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD0415/CD1112 family protein"
                     /protein_id="HLS52344.1"
                     /translation="MQSIIDSITQWLKELLVGGITDNLSGMFDNVNQKVGEIAGEVGM
                     TPSVWNGGVFSMIRNLSETVIIPIAGIILTFIMCYELIQMVIEKNNMHDFETFMFFKW
                     IFKTFVAVMIITNTFNIIMGIFDLSQHVVSGAAGVIISDTNIDISSAIADMETRLLAM
                     ELGPLFGLWLQSMFVGFTMNALTICIFVVIYGRMVEIYLMISLAPIPFSTMANREWGS
                     MGQNYLKSMFALGFQAFLIMVCVGIYAVLVQSIATDADIMSAIWTCVGYTVLLCFTLF
                     KTGSLAKSIFSSH"
     gene            1605..1982
                     /locus_tag="VK031_00090"
     CDS             1605..1982
                     /locus_tag="VK031_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HLS52345.1"
                     /translation="MAYVPIPKDLSTVKTKVMFNLTKRQLICFSIALVVGLPIFFLIK
                     DSTGTSTATLIMIFSMLPFFMFAMYEKHGQPLELILKHYIEVRFIKPKYRPYKTKNFY
                     SLLIRQNQLDEEVKYIVKKAKSR"
     gene            1957..>2648
                     /locus_tag="VK031_00095"
     CDS             1957..>2648
                     /locus_tag="VK031_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HLS52346.1"
                     /translation="MLKKQNQGSPKLTREEKRLINNAIVRARGNNKKEQSAQDTIPYE
                     RMYPDGICRVTDKLYTKTIRFQDINYQLAQNEDKTAIFESWCDFLNYFDSSIKFQLSF
                     LNMTANTKDYESSIHIKSQEDDFDSIRTEYTQMLHNQLSKGNNGLMKSKYLTFGIEAD
                     SIKIAKPRLERIENDLWNGFKRLGVVVDSIEGKERLKLCYNILHMDGNVPFNFDWKWL
                     APSGLSTKDFIAP"
BASE COUNT          873 a          433 c          540 g          802 t
ORIGIN      
        1 atgcggatac catcatcggc aactgtgata gtatgatctt ccttggtggt aaggaaaaaa
       61 ctaccctcaa ggatttatca gaaaccctcg gaaaagaaac cattgatatg tttaatactt
      121 ccgacaccag agggacacaa aggagttatg ggcttaatta tcaaaagctg ggaaaggagc
      181 ttatgagtat ggatgaactt gctgttatgg atggaggcaa gtgcatttta cagctgaggg
      241 gtgttcgccc atttctatcg gacaagtacg acattacaaa gcatccaaac tacaaatact
      301 tatccgactt tgataaaaga aacaccttca atattgaaaa atatctatcc acaaaactaa
      361 aggtaaaatc taaggaggtt tataatgttt atgaaataga ccttgcagat gaaaacaaaa
      421 ccgctgacta ggcggttatt ttaattcaaa ataaaataaa ttttaggagg aaataattat
      481 ggcatttttt aattcagcag tagacgtatt gcaaacattg gttattgctc ttggtgcagg
      541 acttggtatc tggggagtta ttaatctttt agaaggttat ggtaatgaca accccggtgc
      601 aaaatcacaa gggatgaaac agctaatggc aggcggtggc gtagcactga ttggcatgac
      661 ccttgtaccc cttctttcag ggttatttgg ttaaggtaaa agattatgca aagcatcatc
      721 gacagtatta ctcagtggct caaagagcta ttagttggcg gtataacgga taacttgtcg
      781 gggatgttcg ataatgtcaa ccaaaaggtt ggcgaaatcg caggtgaagt aggaatgaca
      841 ccgtcagtat ggaatggcgg tgtcttttcc atgatacgaa acctctccga aacagtgatt
      901 attcctattg ccggcattat cttgaccttt atcatgtgct acgaactgat acaaatggtc
      961 atagaaaaga ataatatgca tgactttgaa acctttatgt ttttcaaatg gatatttaaa
     1021 acctttgtgg cagtaatgat aataaccaac acctttaata tcattatggg aatctttgat
     1081 ttgtcccagc atgttgtctc aggtgcagca ggtgttatta ttagcgatac caatatagat
     1141 ataagttctg ccatagcaga tatggaaaca cggcttttgg caatggaact tggtcctctc
     1201 tttggcttat ggctacaaag tatgtttgtt ggatttacca tgaatgccct tactatctgt
     1261 atttttgtag ttatctatgg gcgaatggta gaaatatatt taatgatatc ccttgcaccc
     1321 atcccctttt ctacaatggc aaatagggag tggggcagta tggggcaaaa ttatttaaaa
     1381 tccatgtttg cccttggttt tcaagccttt ctcattatgg tatgtgtagg gatttatgca
     1441 gtattagtcc aatccattgc cacagatgct gatattatga gtgcaatatg gacttgtgta
     1501 ggctatacag ttttactttg ctttacccta tttaagacag gctcactggc aaaaagtatt
     1561 tttagttccc attaaaataa aaattaaatg gaaaggagga ttttatggca tatgtaccta
     1621 tacccaaaga tttaagtacc gtaaaaacca aggtaatgtt taaccttacc aaaaggcagt
     1681 tgatctgttt ttccattgcc cttgtggtag gcttaccgat attttttctt ataaaggaca
     1741 gcactgggac aagtacagct acacttataa tgattttttc tatgttgccc ttttttatgt
     1801 ttgctatgta tgaaaagcat gggcaaccac tggaattaat attaaagcat tatatagagg
     1861 tgcggtttat aaagcctaaa tacagaccct ataaaacaaa gaacttctat agccttttaa
     1921 ttaggcagaa tcagctagat gaggaggtaa agtatattgt taaaaaagca aaatcaaggt
     1981 agtcccaagc taacaagaga agaaaaaaga ttaattaata atgccatagt aagggcaagg
     2041 ggaaataata aaaaggaaca atcagcacag gataccatcc cttatgagcg tatgtatcct
     2101 gatggaattt gcagggtaac agataagctc tataccaaaa caatccgttt tcaagatata
     2161 aactatcagc ttgcccaaaa tgaagataag acagcaatct ttgaaagttg gtgtgatttt
     2221 cttaactact ttgatagttc tattaaattt caactttcct ttcttaatat gacggctaat
     2281 acaaaggatt atgaaagcag tattcatatt aaatcacagg aagatgattt cgatagtatc
     2341 cgtactgaat atactcagat gcttcataac caactttcta aagggaataa tggcttaatg
     2401 aaaagtaagt acctgacctt tggaatagaa gccgatagta taaagatagc aaaacctcgt
     2461 ctggagcgta ttgaaaatga cctatggaat ggatttaaaa gattgggggt ggtagtagat
     2521 tctatagagg ggaaggaaag attaaagcta tgctataata tcttgcatat ggatggaaat
     2581 gtaccattta attttgattg gaaatggctt gctccatcag gcttatccac caaagacttt
     2641 attgctcc
//
LOCUS       DATGSI010000007         1280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_47959, whole genome shotgun sequence.
ACCESSION   DATGSI010000007 DATGSI010000000
VERSION     DATGSI010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47959"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="VK031_00100"
     CDS             <1..1079
                     /locus_tag="VK031_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLS52347.1"
                     /translation="RKAALEIEADIYIITRDNVIWLVDYLDEWDFDTLVIDELSSFKN
                     HQSKRFKKLRTVTPYFKRVIGLTGTPAPNGYMDLWAQIYLLDRGERLGKNITAYRRRF
                     FNAYYRGQYTEYKLRPGAKEEIDKLISDICISMKAKDYLDLKEPIFIDEKVTLDKKEF
                     KLYQKMAKEAIININDEDIPALSAATVSNKLLQLANGAIYDDDKNYHLVHDKKMKALE
                     ELIEQSAGENVLVFYNFSSDLERIQEKFKDEVVVLETDKDIKDWNKGKIKILLAHPAS
                     AGHGLNLQDGGSIIIWFSLNWSLELYLQANARLHRQGQKNTVRIYRIIAKDTIDEKVL
                     EVLEGKNIRQEELLKKLKAELLGG"
     gene            1083..1277
                     /locus_tag="VK031_00105"
     CDS             1083..1277
                     /locus_tag="VK031_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52348.1"
                     /translation="MDKLLELCLRSIIRNINNDHMIAKIYRNKALEIHKEIEFEESCR
                     YPIGHVIPESTKLKLYEMVS"
BASE COUNT          539 a          150 c          252 g          339 t
ORIGIN      
        1 agagaaaagc agcattagaa atagaagctg atatttatat aatcactaga gataatgtta
       61 tatggcttgt agattattta gatgaatggg attttgatac tttagttata gatgaattat
      121 caagctttaa aaaccatcaa agtaaaagat ttaaaaaact aaggacagta actccatatt
      181 ttaaaagagt aataggcttg acaggtaccc cagcaccaaa tggatatatg gatctatggg
      241 cacaaatata tctattggat aggggggaaa ggttgggaaa gaatatcact gcttatagga
      301 gaaggttctt taatgcctac tatagaggac agtacacaga atataaatta agaccaggag
      361 ccaaagaaga gatagacaag cttatttctg atatatgtat ttctatgaaa gcgaaagact
      421 acttagattt aaaagaacca atctttatag atgaaaaagt taccttagat aaaaaggaat
      481 ttaaactata ccagaaaatg gctaaagagg caattataaa tataaatgat gaagatatac
      541 cagcattgtc agcagccaca gtatccaata aattattaca attagcaaat ggggccatct
      601 atgatgatga taaaaactat cacctagtac atgataaaaa aatgaaagca ttagaagagt
      661 taattgagca aagtgcagga gaaaatgtat tagtatttta taattttagt tccgacttag
      721 agaggataca agaaaaattt aaggatgaag tagtagtctt agaaactgat aaagatataa
      781 aggattggaa taagggtaaa attaaaatct tattagcaca tccagccagt gcgggacatg
      841 gactaaatct tcaagacgga ggaagtataa taatttggtt tagtctaaat tggtccttag
      901 aactatatct acaagcaaat gcaaggcttc ataggcaagg tcagaaaaat acagttagga
      961 tatacaggat tatagctaaa gacactatag acgaaaaagt attagaagtc ctggaaggta
     1021 aaaacataag acaggaagag ctattaaaga aacttaaagc tgaattgtta gggggttaaa
     1081 aaatggacaa actgttagag ttatgcctta gaagtattat tagaaatata aataacgatc
     1141 atatgatagc taagatatac agaaataaag cattggaaat acacaaggaa attgaatttg
     1201 aggaaagttg cagatatcca atagggcatg taattccaga atctacaaaa ctaaaactat
     1261 acgaaatggt gagttaagat
//
LOCUS       DATGSI010000008         1282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_782, whole genome shotgun sequence.
ACCESSION   DATGSI010000008 DATGSI010000000
VERSION     DATGSI010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..>1282
                     /gene="mnmE"
                     /locus_tag="VK031_00110"
     CDS             122..>1282
                     /gene="mnmE"
                     /locus_tag="VK031_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HLS52349.1"
                     /translation="MTEYSIIITISNENIIEVNKVETIAAISTAMGEAGIGIVRLSGS
                     DAINIGNKVFKGVKGKKLTEAKNRTLSYGHIVDHENDLIIDEVLIAYMKEPYTYTREN
                     MVEIYCHGGSISVRRILELLLNNGARLAEPGEFTKRAFLNGRLDLSQAEAVIDIIKAK
                     TNKSFQVSFNQLEGSLSEQIGEIRDILLGLLAHVEASIDFPDEDLEEIAYEELLENSL
                     VVEKRIEKLLATADRGKILRDGLNTVILGKPNVGKSSLLNAILRENRAIVTDIPGTTR
                     DIIEEYVNIDGIPLRIIDTAGIRETKDEVERIGVDRAKQVVENADLIIAVFDVSRDLS
                     QEDYEIIDLIKDRRSIVILNKTDLPEKFSADTIKDLMGEKELIKTSLTEGIGI"
BASE COUNT          489 a          150 c          285 g          358 t
ORIGIN      
        1 gtgaaggtag tgaaccctat agaagggtag ttatccaatc taagtaattt tttatcccat
       61 tgattaatca atgggatatt tttttgtgta tctaattaag ctataagaga aatataataa
      121 tttgacagag tatagtataa taattacaat tagtaatgaa aatataatag aggtgaataa
      181 ggtggaaaca atagcagcca tatctactgc catgggagag gcaggtatag gtatagtaag
      241 gctaagtggt tctgatgcca taaacatagg taataaggtt tttaaaggag ttaaaggaaa
      301 aaaactaaca gaggctaaaa acagaacatt aagctatgga catattgtag atcatgaaaa
      361 tgatttgatt atagatgaag tattgattgc ctatatgaaa gagccctaca catacacaag
      421 ggaaaatatg gtagaaattt attgtcatgg tggttcaatt tcagttagaa gaatattgga
      481 attactttta aacaatgggg ccagattagc agagccagga gaatttacca aaagggcctt
      541 tttaaatggt agactagatt tatcacaggc ggaggcagta attgacataa tcaaggccaa
      601 gactaataaa agctttcaag tatcttttaa ccaattggaa ggtagcctat cagagcagat
      661 tggagaaata agagatattt tgttaggcct attagcccat gtggaggcaa gcatagattt
      721 tcctgatgaa gatctggaag agattgccta tgaagaactg ttggagaatt ctttagtggt
      781 tgaaaaaaga attgagaagc ttttagccac tgctgatagg ggaaaaattt taagagatgg
      841 cctaaacact gtgatcttag gtaagccaaa tgtaggaaaa tcttcacttt taaatgcaat
      901 tttaagagaa aacagggcta tagtaacaga tatacctgga actaccaggg atattataga
      961 ggaatatgta aatattgacg gaattccttt aagaattata gatacagcag gaattaggga
     1021 aacaaaagat gaggtggaaa ggataggtgt agacagggcc aaacaagtag tagaaaatgc
     1081 agatttaata attgctgttt ttgatgtttc aagggatctg agccaagaag attatgaaat
     1141 aatagatctg atcaaggata gaaggtcaat agtaatttta aataaaacag acctacctga
     1201 aaagttttcg gctgatacta tcaaggacct aatgggagag aaagaattaa taaaaacttc
     1261 tcttactgaa gggattggaa tt
//
LOCUS       DATGSI010000009         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_283795, whole genome shotgun sequence.
ACCESSION   DATGSI010000009 DATGSI010000000
VERSION     DATGSI010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283795"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..>1070)
                     /locus_tag="VK031_00115"
     CDS             complement(102..>1070)
                     /locus_tag="VK031_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HLS52350.1"
                     /translation="AEDIVKASEETGGIISLEDLENYEVKVREPVKGSYRGYDIISAP
                     PSSSGGTHVIQLLNILENFDMTEKGHNTVESLHLWSEAAKLVFADRAEYMADTDFVEV
                     PLAGLASKDYAKELVGKIDLEEAAQDVEFGDPSKYESGSTTHYSVMDKEGNIVAVTET
                     INYFFGSGVTVDGRGFILNNEMDDFNAEPGTSNSVEPGKRPLSSMTPTIVLKDNEPFL
                     ALGSPGATRIIPTVAQIISNIIDHGMNVQEAINAPRMFDMRGTLSVEGRMEEDVIKGL
                     EDLGHEVDVKGDLDPYFGGAQCIMMEESGRLHGGGDPRRDGQAIGY"
BASE COUNT          280 a          253 c          149 g          388 t
ORIGIN      
        1 ccaaacagta gtaaggataa acttaaaact agagcaatta atactaacat atttttattt
       61 ttaagcacta tccaacactc cttgaaaact tatttataac tttaatatcc tattgcctga
      121 ccatctcttc ttggatctcc accgccatgt aatcttcctg attcctccat cataatacat
      181 tgagctcctc caaagtatgg atccaaatct ccctttacat caacttcatg gcctaaatct
      241 tctaatcctt taattacatc ttcttccatt cttccctcca ctgatagggt tcctctcata
      301 tcaaacattc tgggtgcgtt tattgcttct tgtacattca ttccatgatc tataatatta
      361 cttataattt gtgctactgt tggtattatc cttgttgcac ccggtgatcc tagtgctaag
      421 aatggttcgt tgtcctttag aactatagta ggcgtcatac tactaagtgg tctcttacct
      481 ggttctacag agtttgaagt tccaggttca gcattaaagt catccatttc attattcaat
      541 ataaaccctc tgccgtctac tgtaactcct gagccgaaga aatagtttat agtctcggtt
      601 actgccacaa tatttccttc cttatccatt actgaatagt gagtagtact tccactttca
      661 tattttgatg gatcaccaaa ttctacatct tgggctgctt cttctagatc aatctttcca
      721 actagttctt tggcatagtc cttagatgca agtccagcta atggtacttc tacaaaatca
      781 gtatccgcca tatactctgc cctgtctgca aatactagtt ttgctgcctc agaccataga
      841 tgtaatgatt ctactgtatt atgtcctttt tctgtcatgt cgaagttttc taatatattt
      901 aacaattgga taacgtgggt tccacctgaa ctggatggtg gagctgatat aatgtcatag
      961 cccctataag atcctttaac cggctctctc acctttacct cataattttc caaatcttct
     1021 aatgatatta ttccgccagt ttcctctgaa gccttaacta tgtcttctgc
//
LOCUS       DATGSI010000010         1175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_94964, whole genome shotgun sequence.
ACCESSION   DATGSI010000010 DATGSI010000000
VERSION     DATGSI010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94964"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VK031_00120"
     CDS             <1..295
                     /locus_tag="VK031_00120"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HLS52351.1"
                     /translation="QVKDNKALEMLQSQGTKSILEAIRHELENIEEIDKEFAASLMKK
                     VQKATGIRGKNLFMPTRVALTGSLHGPEFVKVIYLLGKENLLERIEYVEKNYF"
     misc_binding    331..540
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            694..>1175
                     /locus_tag="VK031_00125"
     CDS             694..>1175
                     /locus_tag="VK031_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HLS52352.1"
                     /translation="MKLYNSLTRKKEKFVPVKENQVSMYNCGPTVYDYIHVGNARPLV
                     VFDTLRRFFIYKGYDVKFVVNFTDIDDKLIDKANKEATTVKKIAERYIEAFMEDAKGL
                     NLYDYKTINPRATEYISQMIDFIQGLIDKNAAYNVDGNVYFNIKSAKDYGKLSKKNID
                     "
BASE COUNT          442 a          139 c          252 g          342 t
ORIGIN      
        1 ccaagtaaag gataataagg ccctagaaat gctacaaagc cagggtacca aatcaatttt
       61 ggaagccata agacatgaac tagaaaatat agaagagata gataaggaat ttgcagcatc
      121 tctgatgaag aaggtacaaa aagccactgg aataagaggg aaaaatttat ttatgcccac
      181 cagggtagcc ttaacaggca gcctgcatgg accagagttt gtaaaggtaa tctatttgtt
      241 aggcaaggaa aatctcttag agagaattga gtatgtggag aaaaattact tttagataga
      301 tttataattt aaagataaaa aagaaaaatg ataagggtaa ggatgggaag agtagattta
      361 ctggatctta cagagaggga ttctcatagg ctgagaggga tcctaatgta ttggtaaatc
      421 gaagtgcatc cctgaaccat aacctgaatt attagggttt atcgactggc agcgttatat
      481 gccgaaagtg ggcttatgcc tattagagtg gaaccgcgga agtaaccttt cgtctctaaa
      541 tttgagatgg aaggtttttt tattttaaat ttttatataa gatatataga aaaggcccat
      601 atatatcata tatgaataag tttttataaa ctttttagga attaattata gagatagtta
      661 gaagttataa tataatttta aaggagtgat tttatgaaac tgtacaatag cttaactagg
      721 aagaaggaaa aatttgttcc agtaaaagaa aatcaggtga gcatgtataa ttgtggacct
      781 acagtatatg attatataca tgtgggaaat gctagacctt tagtagtctt tgatacacta
      841 cgtaggtttt ttatatataa gggatatgat gttaaatttg tggtaaactt tacagacata
      901 gatgataagt tgattgacaa ggctaataag gaagctacta cagttaaaaa aattgccgaa
      961 agatatattg aggcctttat ggaggatgcc aagggattaa acctgtatga ctataagacc
     1021 ataaacccta gggctacaga atatattagc cagatgattg attttataca ggggctaata
     1081 gataaaaatg cagcctacaa tgttgatggc aatgtgtatt ttaatattaa aagtgccaag
     1141 gattacggaa aattatctaa aaagaatatt gacga
//
LOCUS       DATGSI010000011         1241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_142660, whole genome shotgun sequence.
ACCESSION   DATGSI010000011 DATGSI010000000
VERSION     DATGSI010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142660"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..486
                     /locus_tag="VK031_00130"
     CDS             202..486
                     /locus_tag="VK031_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52353.1"
                     /translation="MDKKRILNSLGIYERYNEYTIVEKEDEYEVYRIMTGDIEGCVLV
                     GYNYKDLDGDGLHDVTDSRAFLNKMLTLLPENEKFILRIEDDNPLIINKK"
     gene            522..908
                     /locus_tag="VK031_00135"
     CDS             522..908
                     /locus_tag="VK031_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52354.1"
                     /translation="MYSTCVEYEGELPENSKLKGKCPSVEDTTEELYEKVSEIEKTID
                     IKDIEENCIVHEGITVVEYIEKMATEICNLKSKVEEQETFLNTEITEEIDLKCFEGVV
                     TLKDLLQKIINNNCSSDDNCSTENNW"
     gene            910..1122
                     /locus_tag="VK031_00140"
     CDS             910..1122
                     /locus_tag="VK031_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52355.1"
                     /translation="MNCKDVYKPEYSTKEDCEIVSSDCISFPEDIPYLGISKGESLTE
                     VINKLYEAVRDSRKRITELENQEQNE"
BASE COUNT          535 a          136 c          243 g          327 t
ORIGIN      
        1 ttaacgcatc taaaaatata ctaaccgagg gcattcggtt ataccgtact aagcataaga
       61 cttcattaga agcagtttag gtttgaaagt aagaatctca ccgtctttag acgtgagagt
      121 atgtcaatag aggagatagg ttttcttttg aagaaatgaa agaaatagga aaaacaataa
      181 aacaaataga taaaaaaaga aatggacaag aaaaggatat taaatagttt aggaatatat
      241 gaaaggtata acgagtatac tattgtagag aaagaagacg agtacgaagt atataggatt
      301 atgactggag atatcgaggg atgcgtactt gtgggctata actataaaga cttagatgga
      361 gacgggttac atgatgtaac agatagtagg gcttttttaa acaagatgct gactctttta
      421 cctgaaaatg aaaaatttat attaagaata gaagatgata accctttaat aattaataaa
      481 aaatgacttg tcataaaata aagcatagat gtacagatac agtgtactct acttgtgtag
      541 agtatgaagg agaattacct gaaaattcta agttgaaagg aaagtgtcct agcgtagaag
      601 acactacgga agagttgtat gaaaaagtat ccgagataga aaaaacaata gacattaaag
      661 acatagaaga aaattgtatc gtacacgaag gaataaccgt agtagaatat atagagaaaa
      721 tggctacaga aatttgtaac ctcaaatcta aagtagaaga acaagagaca tttttaaata
      781 cggaaataac agaagaaata gatttaaaat gttttgaagg agttgtgact ttaaaagact
      841 tactacaaaa aataattaat aataattgca gttctgatga taactgctct acagaaaata
      901 actggtaata tgaattgtaa agacgtatat aaacctgagt acagtacaaa agaggattgt
      961 gaaatagtat cttcagattg tatatccttt ccagaagata taccttactt aggcataagt
     1021 aaaggagagt cactaacaga agttattaat aaattatatg aagctgttag ggattctaga
     1081 aaaagaataa cagaattaga aaatcaagaa caaaatgagt aaatgtagaa aatgtaatcc
     1141 tgcaagatac caaggaatag acttagaatg tatacatgct ataaaaggag actgttacga
     1201 tgacatattc aagaacatag atgatatagt atgtaacata c
//
LOCUS       DATGSI010000012         1102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_568037, whole genome shotgun sequence.
ACCESSION   DATGSI010000012 DATGSI010000000
VERSION     DATGSI010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568037"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /gene="fni"
                     /locus_tag="VK031_00145"
     CDS             complement(<1..963)
                     /gene="fni"
                     /locus_tag="VK031_00145"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HLS52356.1"
                     /translation="MENRKDDHVKLAEKFHNSHKISDFDNIRFVHHAFPEIGLGDVDI
                     STEFASFKLNQPFFINAMTGGSEKTRLINEKLSIVARETELPIASGSLSAAIKDPTVM
                     DSFDIIRKTNPKGLVFANLGAEHSVENVKRAIDILKADAVQIHCNVVQEIIMPEGDKD
                     FSNWLTNIEKIVKEIDIPVIVKEVGFGMSKESIKQLVDIGVKTIDVGGKGGTNFAQIE
                     NYRRENKSYYYLEDFGQSTAISLLEAESYIEQVDIIATGGIRNPLDIAKSLALGAKAV
                     GIAGTFLNMVLNQGVDKTIENINNWKEELASITTLLGKKNVGQLV"
BASE COUNT          294 a          248 c          159 g          401 t
ORIGIN      
        1 taccaattgc cctacattct ttttcccaag taaggttgtt atagatgcca gttcttcctt
       61 ccagttattt atattttcta tggttttatc cacaccctgg tttaaaacca tatttaaaaa
      121 ggtgccagct attcccacag ccttagcccc caaggccagg gacttggcaa tatccagtgg
      181 atttcttata cctcctgtgg caattatatc tacctgttct atatagcttt ccgcctctaa
      241 tagggatatg gctgtagatt ggccaaagtc ttctaaatag taatagcttt tattctccct
      301 tcgatagttt tctatctgag caaaatttgt tccgcctttt ccgcctacgt ctatagtttt
      361 tacccctata tccaccaact gcttaataga ttccttactc ataccaaagc ctacttcttt
      421 tactataact ggtatatcta tttccttaac tattttttct atgttggtca gccaattgga
      481 aaagtcctta tctccctctg gcataataat ttcctgcacc acattacagt ggatttgaac
      541 tgcatctgcc ttaagtatgt ctatagccct ttttacattt tccactgaat gttctgctcc
      601 caaattagca aataccaggc ctttaggatt ggtttttctt ataatatcaa agctgtccat
      661 aacagttgga tccttaatgg cagcactaag agaaccagag gctataggta gttctgtttc
      721 tcttgctact atgcttaatt tttcattgat taatcttgtt ttttcactgc ctccagtcat
      781 ggcatttata aagaagggtt gatttaattt aaaggaggca aattcggtgg aaatatccac
      841 atcccccaaa cctatttctg gaaaggcatg gtggacaaac cggatattat caaagtctga
      901 tattttatgg ctattatgaa atttttctgc caatttaaca tgatcatctt ttctattttc
      961 caatattttc acctactttt catatacagt caaaggtaga tattttattc cagccttttc
     1021 ccaatcttta actaaattat ttaagtctaa atcctggtta aagattgcaa tcccacagtc
     1081 tcccccacca gcacctgaaa gt
//
LOCUS       DATGSI010000013         1304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_1142, whole genome shotgun sequence.
ACCESSION   DATGSI010000013 DATGSI010000000
VERSION     DATGSI010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VK031_00150"
     CDS             <1..959
                     /locus_tag="VK031_00150"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HLS52357.1"
                     /translation="KYELYLTVSMGISIYPQDGENPDLLIKHAKDALFQARERGRNSY
                     ILYSAGTDIDSFRKFQLQNDLHLSIEREQLVLNFQPIVKMETGEILAAEVLVRWVHPT
                     WGIVGPDEFIPMAEETGFVIDLGKWILKETCRIYKKWLVESLPAIKIGINYSTLQLYE
                     KDFARKIGNIIKKFQLDPSFLIMEITESILMQDSKRVNGDIKQLQDLGIQIALDDFGT
                     GYSSLSYLGSFNIDILKIDRSFIENIPYNKTNTVIVEFLIKMAKDLNLKLVAEGIENW
                     DQFLFLKNLNCHTGQGYIFGKPVSKEEFKRILAKNDCRPFSK"
     gene            997..>1304
                     /locus_tag="VK031_00155"
     CDS             997..>1304
                     /locus_tag="VK031_00155"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLS52358.1"
                     /translation="MSLEYVESPEKQNRKMKNFNELVQTFIDASKDMVFLKDDKLSYI
                     FVNQAFKNFYKLDESEILGKKDHDLIDKKLAQIYTDIDRKVLDKLITLETEIEQDKKV
                     "
BASE COUNT          487 a          177 c          259 g          381 t
ORIGIN      
        1 gtaagtatga attatattta acagttagca tgggaatcag tatttatccg caggatgggg
       61 aaaatccaga cctccttata aaacatgcta aagacgccct cttccaggcc agggaaaggg
      121 gaagaaatag ctacatatta tattctgctg gaacagatat tgatagcttc agaaagttcc
      181 aactacaaaa tgacttgcac ctatctattg aaagagagca actggtctta aattttcaac
      241 ctatagttaa gatggaaaca ggagagattc tagcagcaga agtactagta agatgggttc
      301 accctacctg gggtattgta gggcctgatg aatttattcc aatggctgaa gagacaggct
      361 ttgtaataga cctgggtaag tggattttaa aagagacctg taggatatat aaaaaatggc
      421 tggtggaaag cttgccagct ataaagatag gtataaacta ttctacccta cagctatatg
      481 agaaggattt tgccaggaag attggaaata ttattaaaaa attccagcta gaccctagct
      541 ttttaattat ggagattaca gaaagtatcc tgatgcaaga ttctaaaagg gttaacggtg
      601 acataaagca attacaggat ctagggatcc aaatagcctt agatgatttt gggacaggct
      661 attcttccct atcgtatcta ggctccttta atatagatat attaaaaata gatcgttcat
      721 ttatagagaa tataccttat aataagacta atacagtaat tgtagagttt ttaataaaga
      781 tggccaagga tctcaatcta aagttggtgg ctgaaggaat agagaattgg gatcaattct
      841 tatttttaaa aaatttaaac tgccatactg gccaaggtta tatatttgga aaaccagtct
      901 ccaaggagga atttaagaga attttggcca aaaatgattg tagaccattt agtaaataaa
      961 tatatttaag atataatctt aataggaagt ggtattttgt cattagaata tgtagagagt
     1021 cctgaaaaac aaaatagaaa aatgaaaaat tttaatgaac ttgtacaaac ttttatagat
     1081 gctagtaaag atatggtctt cttaaaggat gacaaactaa gttatatttt tgtcaatcaa
     1141 gcctttaaaa atttttataa gctagatgag tcggaaatat taggaaaaaa agatcatgac
     1201 ttaatagata agaagctggc ccaaatctat acagatatag atcgcaaggt tttagataag
     1261 cttatcactt tggaaacaga gatagagcaa gataaaaagg tcta
//
LOCUS       DATGSI010000014         1202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_425877, whole genome shotgun sequence.
ACCESSION   DATGSI010000014 DATGSI010000000
VERSION     DATGSI010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425877"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /gene="rimI"
                     /locus_tag="VK031_00160"
     CDS             <1..277
                     /gene="rimI"
                     /locus_tag="VK031_00160"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HLS52359.1"
                     /translation="AFKKELNNDVARYLVAKVDGKIVGYVGIWFVMDEGHITNVAVHS
                     DYRGRKIGDKLVQALVELCRENKIVSMTLEVRVSNIVAQNLYKKYGF"
     gene            361..>1202
                     /gene="tsaD"
                     /locus_tag="VK031_00165"
     CDS             361..>1202
                     /gene="tsaD"
                     /locus_tag="VK031_00165"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004812935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HLS52360.1"
                     /translation="MKDIITLAVESSCDETSVAVLKNGREVLSNIINTQVDLHKKFGG
                     VVPEVASRKHIESINFIIQQALDEADCSFQDIDLIGVTQGPGLVGALLVGISAAKALA
                     YGLDRPLIGVNHIEGHVCANYIEHKNLKPPFTCLIVSGGHTYLVQANNYTDYELIGRT
                     RDDAAGEAFDKVARALGLPYPGGPFIDKLSKKGDSKAIDFPRVFLEDDSYDFSFSGLK
                     TAVLNYLNHAKQKGKEIIIEDVAASFQQAVLDVLVEKTFRLAKERGSEKIVFAGGVAA
                     NSGL"
BASE COUNT          439 a          159 c          271 g          333 t
ORIGIN      
        1 tgcattcaaa aaagaattaa ataatgatgt tgcaagatat ttagtagcca aagtagatgg
       61 gaaaattgtt ggatatgttg gtatatggtt tgtaatggat gaaggtcata ttacaaatgt
      121 tgctgttcat agtgattata gaggaagaaa aatcggagat aaattagtac aagctttagt
      181 agagctttgt agagaaaata agatagtatc aatgacatta gaagttagag tttctaacat
      241 agtagcacaa aatctttata aaaaatatgg attttagcta gcaggaataa gaaaagaata
      301 ttacagcgat aacaaagaag atgccatgat aatgtggaat gatataaagg tggtgtaaat
      361 atgaaagaca taataacatt agctgttgaa agtagttgtg atgaaacatc tgttgcggtt
      421 ttaaaaaatg gaagagaagt tttatcaaat ataataaata ctcaagttga tttacataaa
      481 aaatttgggg gtgtagtacc agaggttgca tcaagaaaac atatagaaag cataaatttt
      541 ataattcaac aggccttaga tgaggcagat tgcagttttc aggacataga tttaatagga
      601 gtaacccagg ggccaggcct agtaggtgct ctgctagttg gaatatcagc agccaaggct
      661 ttagcctatg gcttagacag gccattaata ggagtcaatc acatagaagg ccatgtatgt
      721 gccaattata tagaacataa aaatctcaag cctcccttta cctgtctaat agtatcagga
      781 ggccatacct acctggttca ggccaataat tatacagact atgaattaat aggtagaact
      841 agggatgatg cagcaggaga ggcctttgac aaggttgcca gggccttggg cctaccttat
      901 cctggaggtc cctttataga caaattatct aaaaaagggg atagtaaggc catagacttt
      961 cccagggtct ttctagaaga tgatagctat gattttagct ttagtggact taaaacagca
     1021 gtattaaatt atctaaacca tgctaaacaa aaaggaaaag aaataataat agaagatgtg
     1081 gctgccagtt ttcagcaggc agttttagat gtattagtgg aaaaaacctt taggttagcc
     1141 aaggaaagag gatcggaaaa gattgtattt gccggtggtg tagcagccaa ttcaggcttg
     1201 ag
//
LOCUS       DATGSI010000015         1135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_1674, whole genome shotgun sequence.
ACCESSION   DATGSI010000015 DATGSI010000000
VERSION     DATGSI010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VK031_00170"
     CDS             complement(<1..512)
                     /locus_tag="VK031_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLS52361.1"
                     /translation="MKIRPKNVEALVEAALGKRKCDLVIENAKVVNLFTGEIYLGEVG
                     IYDGFIAHIEAEVDGREREKIEAEKYYDAKGQYLIPGLIDAHIHIESTMMTPRNFAKA
                     VIPHGTTTVVTDPHEIANVLGVEGVKYMHESSRDIPMRQYILAPSCVPAVPGKENAGA
                     EFHKEEIEEL"
     gene            complement(722..>1135)
                     /locus_tag="VK031_00175"
     CDS             complement(722..>1135)
                     /locus_tag="VK031_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLS52362.1"
                     /translation="KTLRLLDGIIDIYMPDFKYISHKSGRLYSKVEDYPERAKSALIE
                     MNRQVGGIKIDKRGLVYKGLMIRHLVMPGGFSETKGVLDFIKKNLSEDVLVNIMGQYY
                     PAYRAFDYPEIAKRLDRDEFQKAYEYGKSLGLSLC"
BASE COUNT          315 a          265 c          166 g          389 t
ORIGIN      
        1 aatagctctt ctatttcttc cttatgaaat tctgcccctg cattttcctt gccaggtact
       61 gcaggaacac aggatggggc taaaatgtat tgcctcatag gaatgtcccg gctggattca
      121 tgcatgtatt taactccctc aactcccaat acattggcta tttcatgagg gtctgtaacc
      181 acagtagtag taccatgggg gataactgcc ttggcaaagt tccttggcgt catcatagta
      241 ctttcaatat gaatatgggc atcgattagg ccagggatta gatattgtcc cttggcatca
      301 tagtattttt ctgcttctat cttttccctt tcccttccat ccacctctgc ctcaatatgg
      361 gctataaaac catcatatat acctacttct cccaggtata tttccccagt gaaaagattt
      421 acaaccttgg cattttcaat aaccagatca cattttctct ttcctagggc tgcttcaact
      481 agagcttcca catttttggg cctaattttc acttctgatc tcctcctcta aagttctaaa
      541 gtgtcaccat attttaattt ctcaataaat attattatac cattgtttcc atgttttcca
      601 aaaattaaat atgaaaattt atatatccaa atctcttttt tattacaata aatatgaacc
      661 agttgaacct aggttaaaat ataattagtt aaacctttcc ctagccatat agtattaaac
      721 cttagcatag gcttaagcct aaactttttc cgtactcata ggctttttga aactcatccc
      781 tatctaatct tttggctatt tcaggatagt caaaggccct gtaggctgga taatactggc
      841 ccattatatt gaccaagaca tcttctgata agtttttctt aataaagtct aaaactccct
      901 tggtctcaga aaagccaccg ggcatgacta aatgcctaat cataagcccc ttatagacaa
      961 gccccctttt atctatcttt atccctccta cctgcctgtt catctctatt aaggcacttt
     1021 tggccctttc aggatagtcc tccaccttgg aataaaggcg gccagattta tgacttatgt
     1081 atttaaagtc aggcatataa atatctataa taccatccaa gagccttaag gtctt
//
LOCUS       DATGSI010000016         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_615464, whole genome shotgun sequence.
ACCESSION   DATGSI010000016 DATGSI010000000
VERSION     DATGSI010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615464"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..675
                     /gene="udk"
                     /locus_tag="VK031_00180"
     CDS             61..675
                     /gene="udk"
                     /locus_tag="VK031_00180"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HLS52363.1"
                     /translation="MARPILIGIAGGTGSGKTTVSREILRLINKKDISIIEQDSYYKD
                     QSHLSFDERMKTNYDHPFAFDNKLLVSHLKQLLNSKSIEKPIYDFERHNRKKETILVE
                     ARKIIILEGILILAEKEIRDLLDIKVFVDTDSDVRIIRRIQRDIKERGRSLDSIIHQY
                     MSTVRPAHLQFVEPTKKYADIIIPEGGYNKVAIDLLVSKIKSIV"
     gene            741..>2248
                     /locus_tag="VK031_00185"
     CDS             741..>2248
                     /locus_tag="VK031_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HLS52364.1"
                     /translation="MEKDIKAIIKKRMYMSFILVILIFVLLSFRLYYLQIKQGDHLKL
                     NAIKQRGREFSIYPERGPIFDRHTRPITNNEVESTAIIEKKDLADNALYNRVLSNTSL
                     SRIQLNQLIRNSTDLVQIPLDRVEGLENQGNVFIVDVVKRYSKGNPLAHVVGYINQSE
                     NRGKYGIELIHDEFLRNSDKSSFVLEYDRDRKMILGGTYYVNRNIDPNSPSGVKLTVD
                     LELQKEIEKIMDKFQMNGAVIINQIADGEILALASRPNFNQEKIEEYIDNGDMTFFNK
                     AVQVSYPPGSLFKIVVLLTALEEEPEVLKDKYSCKGYEIINGIQINCSSTHGQLDLKE
                     AFAKSCNSVFIQIGKEIGANRVIKMAEKLGFGSTLGIGLLEKGGILPKRKELFGAAIG
                     NISIGQDKLEVTPLQISNLLTIIANKGIRKDLSIIKGITNKEGVIIKAFQKGEDVRLI
                     STASTELLLEYLKEVVSSGTARSMEVYKIGGAGGKTGSAQATFMKKATVHGW"
BASE COUNT          841 a          313 c          454 g          640 t
ORIGIN      
        1 gaaaatgaaa aagccggtga aaccatttta cctaattaga agagaaagga attgatttta
       61 ttggcaaggc ccatattaat tggaattgca ggcgggacag gctctggcaa aactacagtt
      121 tcaagagaga ttttaagatt aatcaacaaa aaagatattt ccataataga gcaggactca
      181 tattacaagg atcaaagtca cctgtctttt gatgagagga tgaaaaccaa ctatgatcat
      241 ccctttgcct ttgacaacaa attattagta agtcatctga aacaattatt aaatagtaag
      301 tctattgaaa agcctatata tgatttcgaa aggcacaata gaaagaagga aactatcctg
      361 gtggaggcaa ggaaaataat catacttgaa ggtatattaa tcctggctga aaaggaaatt
      421 agggacctgt tagatataaa agtttttgtt gatactgatt ccgatgtaag aattatccgt
      481 agaattcaaa gggacattaa agaaagagga agaagtttgg attctattat ccatcagtat
      541 atgagtactg taaggccagc ccatctgcaa tttgttgaac ctactaaaaa atacgcagat
      601 attataatac ctgaaggtgg ctataacaag gtggccattg atttattggt atcaaagata
      661 aagtctatag tatagagtag aactatctta aagtatactt cctaatttat tgctaacaat
      721 ataaattagg aggttttttt atggaaaaag atataaaagc tattataaaa aaaagaatgt
      781 atatgtcttt tattttggtt atacttatat ttgtcttatt atcctttcgc ctatactatt
      841 tacaaattaa acagggagat catctaaaac taaatgccat taaacaaagg ggaagggaat
      901 tttccatcta tccagaaaga ggtccaatat ttgataggca tacaagacct ataactaaca
      961 atgaagttga gtcaactgcc attatagaaa agaaagattt ggctgataat gctctatata
     1021 atagggtttt aagtaatacc agcttatcta gaattcaact gaaccagctt attagaaatt
     1081 caacagacct ggtacaaata cctttagaca gggtggaggg gttggagaat caaggtaatg
     1141 tttttatagt agatgtagtt aaaagataca gcaaggggaa ccctttggcc catgtagtag
     1201 ggtatataaa ccaatcagaa aataggggaa agtatgggat tgaattgatc cacgatgaat
     1261 ttttaagaaa tagtgataaa tcatcctttg tgcttgaata tgatagagat aggaagatga
     1321 tccttggagg aacctactat gtaaatcgaa atatagatcc taatagtcca tcaggtgtaa
     1381 aattaactgt tgatttagaa cttcaaaaag aaatagagaa aattatggat aagtttcaaa
     1441 tgaatggggc tgttatcata aatcaaatag cagatggaga aattctggct ttggcatcta
     1501 ggcctaactt taaccaggaa aaaatagagg aatatattga taatggagat atgacatttt
     1561 tcaataaggc agtacaggta tcctatcctc caggctctct ttttaaaata gtggtacttt
     1621 taacagcttt ggaggaagaa ccagaagtat taaaagataa atactcctgc aaaggttatg
     1681 aaataatcaa tggtattcaa ataaattgta gcagtaccca tggacagtta gatttaaaag
     1741 aagcctttgc caaatcctgc aactcagttt ttattcagat aggtaaagaa attggagcca
     1801 atagggttat aaagatggca gaaaaacttg gcttcggttc tacacttggg attggccttt
     1861 tagaaaaggg aggaatttta ccaaaaagaa aggaactatt tggggcagct attggaaaca
     1921 tttccatagg ccaggataaa ctggaagtaa cccctttaca gattagcaac ctgcttacta
     1981 tcattgccaa caaaggtatt agaaaggatt tatcaataat taaaggaatt acaaataagg
     2041 agggagtaat aattaaggca tttcaaaaag gtgaagatgt aagactgata tcaacagctt
     2101 ccactgagct acttttagaa tatttaaaag aagtagtatc aagcggtact gccaggtcca
     2161 tggaggttta taaaataggg ggtgctgggg gaaaaactgg gtctgcccag gccacattta
     2221 tgaaaaaagc cacagtccat ggatggtt
//
LOCUS       DATGSI010000017         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_474255, whole genome shotgun sequence.
ACCESSION   DATGSI010000017 DATGSI010000000
VERSION     DATGSI010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474255"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /gene="mvk"
                     /locus_tag="VK031_00190"
     CDS             <1..866
                     /gene="mvk"
                     /locus_tag="VK031_00190"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HLS52365.1"
                     /translation="AMPFSEARVTASISEKKGPVDLDCIYYKGELFKAPEILLGLSRL
                     VEEITKSFNKKLEDFTIRIDSTIPPERGMGSSAAVAIATTRALYKYFNKKLSYEKLTN
                     LANISETVIHGTPSGIDAATIAGEMPLYFIKGEPFKALKLNLDGYLIVADTGEKGQTK
                     ATVASVRNLTKSNPKKYIPLIKELGQLTNQARESIEKNNKKLLGQLMIQAHKNLQDLG
                     VSNQILDRLVEAALDQGALGAKLTGGGRGGCMIALAADKKSADLIAKSLIENGAKNVW
                     IDNLGDDLIDI"
     gene            856..1824
                     /gene="mvaD"
                     /locus_tag="VK031_00195"
     CDS             856..1824
                     /gene="mvaD"
                     /locus_tag="VK031_00195"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HLS52366.1"
                     /translation="MIYKGKARAYANIALIKYWGKRDEKYALPMNSSLSLTLDCFYTE
                     TEVIFSKDLEKDYFYLNKEIQDKKETEKISKFLDLFRKASHIDLRAKVDSINYLPTAA
                     GLASSASGFAALAVALNEATGLKLGNKELSMYARQGSGSATRSLYGGFVEWKKGKDKD
                     SYAVKIDDATWDIGMVIVVVNSKEKQVSSREGMKNTVKTSPFYPAWVENAEKDIIEAK
                     LAIKNKDFERLGLITERNGLMMHATMLGSKPPLSYWEPESVFVMQEVRRLRDEGIPCY
                     FTMDAGPNVKILCRLSDSKRIKEKLLENFKEEQIIISGPGPGPSIL"
     gene            1836..>2367
                     /locus_tag="VK031_00200"
     CDS             1836..>2367
                     /locus_tag="VK031_00200"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HLS52367.1"
                     /translation="MITTKIPGKLFIAGEYAVTEPGNRAILVAVDKFITISLEKKENQ
                     GTIIVDNRAINWTRKNGKIHIDESPRLKYVLKAMELVESYAKELGKEPIYYKLQVDSQ
                     LETREGIKYGLGSSSAVTVAAVDGLCKLYKIDLSKKELFKLAALVNLSINRESSCGDI
                     AACVYGGWIEYRSFNRQ"
BASE COUNT          857 a          370 c          530 g          610 t
ORIGIN      
        1 tagccatgcc cttttcagaa gctagggtaa ccgcaagcat ttcagaaaag aaggggcctg
       61 ttgatttaga ctgtatttat tataaaggtg agctttttaa ggcacctgaa attcttttag
      121 gcctaagtag gctggtcgaa gaaattacta agagctttaa taagaaatta gaagacttta
      181 ccattagaat agatagtacc attccaccag agagggggat gggttccagt gctgctgtgg
      241 ccatagccac cacaagggcc ttgtataagt attttaataa gaaattaagt tatgaaaaac
      301 taacaaattt agccaatata tctgaaacag tgatccatgg aacccctagt ggcatagatg
      361 cggccaccat agctggagag atgcctttat attttattaa aggagagccc tttaaagctt
      421 taaaactaaa cctggatggc tacttaatag tggctgatac aggagaaaag gggcaaacta
      481 aggcgacagt agcctcagtt agaaacctaa ccaaatccaa tccaaaaaaa tatatacctt
      541 taattaaaga gctaggtcaa ttgaccaatc aggccaggga atcaatagaa aaaaataata
      601 aaaagcttct tggccaacta atgatccaag cccacaagaa cttacaggat ctgggggtta
      661 gcaaccaaat actagaccgt ctggttgagg ctgccttaga tcaaggtgct cttggtgcca
      721 aattaacagg aggaggcagg ggcggttgta tgatagcctt ggcagctgat aaaaaaagtg
      781 cagatctcat agccaaaagc ttaatagaaa atggagcaaa aaatgtgtgg atagataatt
      841 taggagatga tttaattgat atataaaggg aaggctaggg cttatgccaa tatagcctta
      901 ataaaatatt ggggcaaaag ggatgaaaaa tatgccctgc caatgaatag tagcctttct
      961 ctcaccttag attgctttta tacagaaaca gaagttatct ttagtaagga tcttgaaaaa
     1021 gattatttct acctaaacaa agagattcaa gataaaaaag aaacagaaaa aataagtaaa
     1081 tttctagatt tatttagaaa agcgtctcat atagatttaa gagccaaggt agatagtatt
     1141 aattacctac ctacagcagc cggcctggct tcttctgcct ctggttttgc agccttggca
     1201 gtagccttaa atgaggccac tggcttaaaa cttgggaata aggaattatc catgtatgca
     1261 agacaaggct caggtagtgc aactagaagt ctttatggag gatttgtaga atggaagaag
     1321 ggcaaggata aggactctta tgctgtaaag atagacgatg ccacttggga tatcggtatg
     1381 gtaatagttg tagtaaattc taaagaaaag caggtatcca gcagagaagg catgaaaaat
     1441 acagtgaaga cttcaccctt ttatcctgct tgggtagaaa atgctgaaaa ggatataata
     1501 gaagcaaaat tagcaattaa aaataaagac tttgaacggc taggcttaat cacagagaga
     1561 aatgggctta tgatgcatgc aaccatgtta ggatctaagc cacccctgtc ctattgggag
     1621 ccagaaagtg tatttgtaat gcaagaagta agaaggctaa gagatgaagg tattccctgt
     1681 tatttcacaa tggatgcagg acctaatgta aaaattcttt gcagactatc tgatagtaaa
     1741 aggataaagg aaaagttact ggaaaatttc aaagaagaac aaataattat ttcagggcct
     1801 gggcctggcc ctagtatatt ataggtggtg gacaaatgat tacaacaaag attccaggga
     1861 agctatttat agctggggaa tatgcagtaa ctgaaccggg aaacagggca atcctagtag
     1921 ctgtagataa atttataact atttccttgg aaaaaaaaga aaatcaagga accattatag
     1981 ttgacaatag ggccataaat tggaccagga aaaatggaaa aattcacata gatgaaagcc
     2041 caaggcttaa atatgtttta aaggccatgg agcttgttga atcctatgca aaagaattag
     2101 gaaaggaacc tatctattat aagttacagg tagatagcca gctggagaca agggaaggta
     2161 taaaatacgg attaggctcc agctccgcag taacagtggc agccgtagac ggtttatgta
     2221 aattatataa gatagattta agtaaaaaag aattatttaa actggctgct ttggttaacc
     2281 tatccataaa tagagaaagt tcctgtggag atattgcagc ctgcgtgtat ggtggttgga
     2341 tagagtatag gtcctttaat agacaat
//
LOCUS       DATGSI010000018         1914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_191178, whole genome shotgun sequence.
ACCESSION   DATGSI010000018 DATGSI010000000
VERSION     DATGSI010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191178"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VK031_00205"
     CDS             <1..836
                     /locus_tag="VK031_00205"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HLS52368.1"
                     /translation="INISRKIQDEKEISRLRHIGKNIMKDNMGLIFRTAALGVDESIL
                     EAEYKSLINIYKEIEIQRNFLPTPKLLYKDLGLIFKLVQDRLDRKDYEIIVNCKDTYK
                     NLLQFGQYLDYSLKDRIKLDKNFSIDYNQTIQEGLTEALKRRVRLASGGHIVIDETEA
                     LTAIDVNTGKFVGNISLEETVFKTNMEAAEEIARQIRLRNLGGIIIIDFIDMKRQKYI
                     NKLLNKLENSFSNHENRANILGMTKLGLVEVTRKRLGPSLKERLTKPCSSCEGQGRIR
                     N"
     gene            966..1277
                     /gene="rplU"
                     /locus_tag="VK031_00210"
     CDS             966..1277
                     /gene="rplU"
                     /locus_tag="VK031_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HLS52369.1"
                     /translation="MYAVIETGGKQYRVEEGDILQVEKLDVKEGETLEFDKVLLVSKE
                     DELLAGRPYVEGAKVVGSVLEQGKGKKIVVYRYKAKKNYSRKQGHRQPFTKVKIEKIV
                     G"
     gene            1280..1636
                     /locus_tag="VK031_00215"
     CDS             1280..1636
                     /locus_tag="VK031_00215"
                     /inference="COORDINATES: protein motif:HMM:NF016233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HLS52370.1"
                     /translation="MITVRIVKDCLGNIKGFNIKGHAGYAAYGEDIVCSAVSILSHTA
                     LLSLVKVAGIEEKDIKYKVEEEKGYLIVTLPKKINKESLDKAQIILQSFELGIGSITE
                     AYPKHVRLQYEEVREC"
     gene            1630..>1914
                     /gene="rpmA"
                     /locus_tag="VK031_00220"
     CDS             1630..>1914
                     /gene="rpmA"
                     /locus_tag="VK031_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HLS52371.1"
                     /translation="MLKLNLQLFATKKGVGSSRNGRDSQAKRLGVKRGDGQYVLAGNI
                     LVRQRGTKIHPGANVGRGSDDTLFATVDGYVKFERMGRDRRKVSVYPEEEL"
BASE COUNT          748 a          274 c          435 g          457 t
ORIGIN      
        1 aaataaatat ttcaagaaag atccaggatg aaaaagaaat atccaggcta aggcatatag
       61 gaaaaaacat tatgaaagat aatatgggcc taatatttag gactgcagcc ctaggtgtag
      121 atgaaagtat tttagaagct gaatacaaga gcctaatcaa tatctacaag gaaatagaga
      181 ttcaaagaaa ttttcttcct actccaaaac tactttataa ggatcttggc ctaatattta
      241 aattggtgca agacagacta gatagaaagg actatgaaat catagtaaac tgtaaagaca
      301 cctataaaaa cctgttacaa tttggccaat atctggatta ttccttgaaa gataggataa
      361 aattagataa aaacttttcc atagattata atcaaaccat ccaggaaggc ctgacagagg
      421 ccctaaaaag aagggtaagg ctggcaagtg gaggtcatat agtcatagac gagacagagg
      481 ccttaactgc tatagatgta aacacaggta aatttgtagg caatatatct cttgaggaga
      541 cagtatttaa aaccaatatg gaggctgcag aagaaatagc cagacaaatt aggctaagaa
      601 atctaggtgg cattataatt atagatttta tagatatgaa aaggcaaaaa tatataaata
      661 aacttttaaa caagctagaa aactcctttt caaaccatga aaatagggcc aacatactag
      721 gaatgaccaa actaggcctg gtggaggtta ctagaaaaag gctgggacct agcctaaagg
      781 agaggcttac caagccctgt tccagctgtg aaggccaggg caggattaga aactaataaa
      841 ttaagaaaaa agtttgacag tatcataaat atttgttaaa atattctggt atgaggccgc
      901 tcaatgtggg tttaaagctt agtcacctaa cattggcgag actgggttag aggaggtgca
      961 acagaatgta cgctgttata gaaacaggag gaaagcaata cagagtagaa gaaggagata
     1021 tccttcaggt tgaaaagcta gatgtaaaag aaggcgaaac attggaattt gacaaggtac
     1081 tacttgtatc aaaggaagat gaactattag caggaagacc ttatgtggaa ggtgccaagg
     1141 tagttggcag tgtactggaa caaggaaaag gaaagaagat cgttgtttac agatacaagg
     1201 ctaagaaaaa ctacagcaga aagcaagggc atcgtcaacc atttactaag gttaagattg
     1261 aaaagattgt aggttaatta tgattacagt taggatagtt aaagattgcc ttggaaatat
     1321 taaaggtttt aatataaagg gccatgcagg atacgcagcc tatggtgaag acatagtctg
     1381 ctctgcagta tccatcctat cccacacagc cttactttcc ctagtgaagg tggcaggcat
     1441 agaagaaaag gatataaaat ataaggtgga agaggaaaag ggatacttaa ttgttacatt
     1501 accaaagaaa ataaataaag agagtttaga taaagcgcag ataattttac aaagctttga
     1561 attagggata ggctccatta cagaggctta ccctaagcat gtaaggcttc aatatgagga
     1621 ggtgcgagaa tgctaaaatt aaacttgcaa ctttttgcca ctaaaaaggg agtaggtagc
     1681 tccagaaacg gtagagatag ccaggcaaaa agattaggag taaaaagagg agacggccaa
     1741 tacgtcctag caggaaacat ccttgttaga caaagaggta caaaaattca ccctggagca
     1801 aatgtaggaa gaggcagtga tgatacctta tttgctacag tagatggata tgtaaaattc
     1861 gaaagaatgg gtagggatag aagaaaagta agtgtatatc ctgaagaaga attg
//
LOCUS       DATGSI010000019         6768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_2638, whole genome shotgun sequence.
ACCESSION   DATGSI010000019 DATGSI010000000
VERSION     DATGSI010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2638"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1812)
                     /locus_tag="VK031_00225"
     CDS             complement(73..1812)
                     /locus_tag="VK031_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HLS52372.1"
                     /translation="MNNKGKSLIIFMLILVLVTSALLYIKNISSVESISYNEFLDLLD
                     KDMVESVKIGDSAQIVVGLKNEKTYKTDNPRTVDFKKELLLHDVKVVEQSINLTALMF
                     FGGLVISLILFILYFRKYMFKNAEREITSLSNMEEMESGTSVKFHHVAGNEEAKENLK
                     EMVDFIKDPDLYLQYGARLPRGVLLYGPPGTGKTLLAKALAGEAGVPIFPVSGSDFIQ
                     VYSGLGASRIRSLFKDAKEAGKSVIFIDEIDSLGKKRKGNNISGSDEGDRTLNALLAE
                     MSGFKENDGIIVVAATNRIDVLDEALLRPGRFDRQIEVQLPDVNAREEILKLHGKNKP
                     ISEEVDFRKLAIDTTYFSGAKLENLLNESAMIAARMKDKEITSKHIDQAYYKVLVGDE
                     KKDRSSIKIRDREITAYHEAGHALVSKLICKNNKVRRVSIIPSTKGMGGFSLNIPPDR
                     MYRSRAQLIGDIKVALAGRAAEEIIFGREEITTGASSDLERATEISLALIGIYGMDDK
                     FGLLNYNVIASKNFQPNVNLIDRANELLNHLYKETINILRDNKEQLDKLARGLLKYES
                     LDEEDIDNILYEY"
     gene            complement(1926..2144)
                     /locus_tag="VK031_00230"
     CDS             complement(1926..2144)
                     /locus_tag="VK031_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52373.1"
                     /translation="MKDYMGEIMDPLEEKKLDEIVQLYKRMEKDLMYKDYEKIFEEIK
                     RERKRLTEEAENILRKKLKGVFNKRKMD"
     gene            2265..2693
                     /locus_tag="VK031_00235"
     CDS             2265..2693
                     /locus_tag="VK031_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52374.1"
                     /translation="MDNKLIFQLLLLLTKNMDALEDRNIYSLESCFQSIKINLAYTRK
                     KVEMAKRLVSYIPQPFYDSYVKSLAITEILMQVLEFQNTYQDSSLESHESPKTVDKVN
                     LDEIVKILREEMAQIKEDELGVVLSVIANLNKDSTLSAGL"
     gene            complement(2714..3613)
                     /locus_tag="VK031_00240"
     CDS             complement(2714..3613)
                     /locus_tag="VK031_00240"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLS52375.1"
                     /translation="MDLLNENENLIIFLCKREGLNLEEFLAKQNLSKRLFRRLYRQKN
                     IYVNGEFVRKDINLLKGDRVAIYMENEEDDTRPEAMDLDILYEDYDMLVLNKPANMVV
                     HPTKSHQTNTLSNGIAHYFKENNIKRKVRFVNRLDMDTTGVLIVAKNPFAHQQLALQF
                     AANSVTRKYLTLVDGIVEKDEGLIDLPIGRKEEGSIRKVIDQNGQRALTRYIVRERFQ
                     NASLLDVEIYTGRSHQIRVHLNSIGHPIIGDSLYNTKSSYIKRQALHSYYLAVKKVRE
                     DKELKFTAPFPADISSLIDLFRS"
     gene            3705..4511
                     /locus_tag="VK031_00245"
     CDS             3705..4511
                     /locus_tag="VK031_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008900846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HLS52376.1"
                     /translation="MNEIRKINLISNRNLESRKTSVALMEKLQARGFKVSEKYSPDAE
                     LNICVGGDGAFLRAVHRSKFSTIPFVGINTGNLGFFQEIKPEKIDDFIARYINNDYVV
                     NEVFLVETQVVTSNKTYSLKAVNEIVVKGMYSKVVHLNLFISGNHLERFSGDGIIIST
                     PAGSTAYNFSAGGSIVYPTVKALQITPIAPINSKAYRSLLNSTVVPGELIITIKPEAR
                     HKNSTLIVKDGIEFKYNNIKAINFRIPEETIYQLSFDKDNYWNNLKNKFL"
     gene            complement(4548..6344)
                     /gene="pepF"
                     /locus_tag="VK031_00250"
     CDS             complement(4548..6344)
                     /gene="pepF"
                     /locus_tag="VK031_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HLS52377.1"
                     /translation="MTKQVLEREDLKKEYTWDVESLFANVEEWEKEFKRVEGLVEKFV
                     AHKGRVGESSQTLLEVLKDRDRLYRSVENVLVYARLRLDEDTRRSKYQSLSDRAMNLY
                     VEVDERTSFVVPEILAIDEEDLKKFLEENQELSLYKQHLDNIMRQKPHVLSSREESIM
                     ARVNLIGMSPQNIYNMLTDADLKFPTIKDEEGKDVEITHGNFIPLMESKDRNVRKAAF
                     QGLYGVYKGFENTLAATLSGELRNNVFNARMRNYSSTREAALSANNIPLSVYDNLIKA
                     VHKNLDSMYKYMEIRKRALGLDELHMYDLYTPIVEDIDFNIPYEEGMELVYKGLEPLE
                     DEYLSVVEKGFKSRWVDVYENRGKRSGAYSSGSYDSKPFILLNYQNTLDSVFTLAHEM
                     GHSVHSYFTRKNQPYIYGNYSIFLAEVASTANEALLLDYMINNTKDKKERVYLINHYL
                     ESFRTTVFRQTMFAEFEKIINEHLEAGHALTAEYLCSTYKELNELYYGPNVVVDDEIS
                     IEWARIPHFYLNYYVYQYATGFSAAIDLTQKILQEKSPAVENYLNFLKSGSSDYPINV
                     LKKVGIDMTTEEPVDNAMDLFARLVNELDELI"
     gene            complement(6356..>6768)
                     /locus_tag="VK031_00255"
     CDS             complement(6356..>6768)
                     /locus_tag="VK031_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLS52378.1"
                     /translation="AMITSAIANNGQMVRPILVKEVINRNDKVIEKFETEIITQSISS
                     STANQVKEMMLEAVETGTGTNAKSKEFKIAGKTGTAENPSGKSHSWFTGFAPYEDPQF
                     VVTVLIEEAGATGGQVAAPIAREILTYAYKNLDI"
BASE COUNT         2017 a         1401 c          969 g         2381 t
ORIGIN      
        1 aataatttct atttacatta tacattattc cataactttt ttcaagctaa caagaaaatc
       61 agattcaact tgctaatatt catataaaat attatctata tcctcttcat ctaaactttc
      121 atattttaac aagcccctag ctaacttatc taactgttct ttgttatccc ttaaaatatt
      181 aatggtctcc ttgtataaat gattaagaag ctcatttgcc ctatctatta aattcacatt
      241 cggttggaaa tttttagaag cgattacatt gtaatttaat agtccaaact tatcatccat
      301 tccatatatg ccaattaaag ccaggcttat ttccgttgcc ctctctaggt ctgaagatgc
      361 gcctgtagta atctcctctc ttccaaatat aatttcctca gcagcccttc ctgccaaggc
      421 tacctttata tctcctatca actgggccct gctcctatac atcctatcag gcgggatatt
      481 taggctaaaa ccacccatac cctttgtact tgggattata cttactcttc ttaccttgtt
      541 gtttttacag attaatttac ttactaaagc gtggcccgcc tcatggtagg ctgtaatttc
      601 tctatcccta atctttatag aactcctgtc ttttttctca tcacctacta ataccttata
      661 gtaggcctga tctatatgct ttgaggtaat ctccttatcc ttcatcctag cagcaatcat
      721 ggcagactca tttaataggt tttccaactt ggcaccactg aaataagtag tatctattgc
      781 cagtttccta aaatccactt cctctgaaat tggtttgttt ttgccatgta attttaaaat
      841 ttcttccctg gcattaacat caggtaattg aacctcgatt tgcctgtcaa acctaccagg
      901 tcttaataag gcctcgtcta aaacgtcaat cctgttggta gcagccacaa ctattattcc
      961 atcattttcc ttaaatcctg acatttcagc cagtaaagca tttagtgttc tgtctccttc
     1021 atcactgcct gaaatattat tacccttcct cttcttgcct aaggaatcaa tttcatcaat
     1081 aaagattaca gattttccag cttcctttgc atccttaaat aaactcctaa tcctgctggc
     1141 ccccagacct gaatagacct gaataaaatc tgaaccagat actggaaaaa taggtacccc
     1201 tgcctctcca gctaaagcct tggccaacaa agtcttacct gtcccgggag gtccgtatag
     1261 aagaactccc ctaggtagtc tggccccata ttgtaaatat agatctggat ctttaatgaa
     1321 gtcaaccatt tcctttaaat tttccttagc ctcttcattg ccagccacat gatggaattt
     1381 tacactggta cctgattcca tctcctccat gttggataaa cttgttatct ccctttccgc
     1441 atttttaaac atatattttc taaaatataa gataaatagg ataagagata taactaaccc
     1501 tccaaaaaac attagggctg tcaaattaat tgactgttcc acaactttaa catcatgtaa
     1561 taatagctcc tttttaaagt ctacagttct gggattatct gtcttatagg ttttttcatt
     1621 cttcagccct accacaatct gggccgaatc acctatcttt actgattcca ccatatcctt
     1681 atctaaaaga tctaaaaatt cattgtaaga aatagattct acgctagata tattttttat
     1741 atataataaa gcactagtta ctaatactaa tataagcata aaaataataa ggcttttccc
     1801 tttattgttc aaaagttttg cccctctcta atgcaagata atgtaatttt agtatagcaa
     1861 atagccataa gcatgtccat ttccttaatc tagcctggtt ggcttccagt tccagataag
     1921 taagtttaat ccattttcct tttattgaat acacctttca gtttctttct taagatattt
     1981 tcggcttctt ctgtcaatct ttttctttcc ctcttgattt cttcaaatat tttttcatag
     2041 tccttataca ttaagtcctt ctccattctt ttatacagct gaactatttc atctaacttt
     2101 ttttcttcaa gtggatccat aatttccccc atataatcct tcataaaatc cccccaactt
     2161 tcacttatat tacaatttat gaaaatttac taaatatagt cctatttaaa caaaatttcc
     2221 aaacctgcat ataataaatt gtaatactag ctggaggtaa aaatatggat aataaattaa
     2281 tttttcaatt gctacttctt ttaactaaaa acatggatgc cctagaggat aggaatatat
     2341 attctttaga aagttgtttt caatctataa agattaactt ggcttataca agaaaaaaag
     2401 tagaaatggc taaaaggctg gtttcctata ttccccagcc cttttatgat tcttatgtaa
     2461 agtctttagc cattacagaa atattgatgc aagtcttgga gtttcaaaat acttatcaag
     2521 attcaagttt agaaagccat gaatctccta aaactgtaga taaggttaac ctagatgaaa
     2581 tagttaaaat actaagggag gaaatggccc agataaaaga agatgaactt ggggtagttc
     2641 ttagtgtgat agccaattta aataaagatt caaccctttc tgctggccta taaataagag
     2701 taagatacaa ccattaggac ctgaataagt ctattaaact acttatatct gccggaaaag
     2761 gggccgtaaa ttttaattct ttatcttccc ttaccttttt tacagccaaa taatatgaat
     2821 gcagggcctg ccttttaatg tatgagcttt tggtattata caggctatcg ccaataatag
     2881 gatggccaat actatttaaa tggaccctga tttggtggga tctgcctgtg taaatttcaa
     2941 catctaagag gctggcattt tggaatcttt cccttactat gtacctggtt aatgcccttt
     3001 gtccattttg gtcaatcact ttccttatgc ttccctcttc cttgcgccct ataggtagat
     3061 ctataagtcc ttcatctttc tccactatcc catctaccag ggtcagatac tttctagtaa
     3121 cagaattagc tgcaaattga agggccaatt gttgatgggc aaaagggttt ttagccacaa
     3181 ttaaaacacc tgttgtatcc atatctaatc tatttacaaa tctaaccttc cttttaatat
     3241 tattttcttt aaaataatgg gctatcccat tggatagggt atttgtctgg tgtgactttg
     3301 tcggatggac aaccatgttt gcaggcttgt ttagtaccaa catatcataa tcttcataca
     3361 aaatatctaa atccattgct tcaggcctgg tatcatcctc ttcattttcc atatagatcg
     3421 ctaccctatc gccttttaaa agatttatat ctttcctaac aaactctcca tttacatata
     3481 tatttttctg tctatatagc cttctaaata gccttttgga taggttttgt ttggctaaaa
     3541 attcttccaa atttaatcct tctcttttgc ataagaatat tataagattt tcgttttcat
     3601 taagtaaatc catatttttc tccaaatcct ttcctgttta ttatataatg tattatagca
     3661 taatcataat aagagtatat tagcctttat ggaggtgtga atttttgaat gaaattagaa
     3721 aaatcaacct tatctccaat cgtaatctag agtctagaaa gacttctgta gccctaatgg
     3781 aaaaactgca agcaagggga tttaaggttt cagaaaaata tagccctgat gcagaactta
     3841 acatatgtgt aggtggagat ggagcttttc tgagagctgt ccatagaagt aaattttcta
     3901 ccataccttt tgttggtata aatactggca atttaggttt ttttcaagag attaaaccag
     3961 aaaagattga tgattttatt gccagatata taaataatga ttatgtggta aatgaagtat
     4021 ttttagtgga gactcaagtt gtcacatcaa ataaaactta ttctctaaaa gcagtaaatg
     4081 aaattgtggt aaaaggtatg tattctaagg tggttcatct taatttgttt ataagtggta
     4141 atcacttaga acgtttcagt ggcgatggca taattatatc tactcctgca ggtagcacag
     4201 cctataactt ttccgcaggt ggcagtatag tttatcccac tgtcaaggcc ctccaaataa
     4261 ctcctattgc ccctattaat tcaaaggctt acagatcctt acttaatagc acagttgtcc
     4321 ctggtgaatt aattattacc atcaagccag aggccagaca caaaaattcc acattgattg
     4381 tcaaggatgg catagagttt aagtataata atattaaggc cattaatttt agaatcccag
     4441 aagaaaccat ctatcaactt agttttgata aagacaatta ctggaataac ttaaaaaata
     4501 aattcttata aaaaaatccc ttcttcagaa gggattttta agattcttta tattaattca
     4561 tctaactcat taaccagcct agcaaacaga tccattgcat tatccaccgg ttcctcagtt
     4621 gtcatatcta taccaacctt ctttaataca tttattggat agtcagaact accactcttt
     4681 aagaagttaa ggtaattttc tacagctgga cttttttctt gtaaaatctt ctgggttaga
     4741 tctattgcag cagaaaatcc agtagcatac tggtatacat aataatttaa ataaaaatga
     4801 ggaattctcg cccattctat ggaaatctca tcatctacaa ccacattagg accgtaataa
     4861 agctcattta attctttgta ggttgagcat aaatattcgg cagttaaggc atggcctgcc
     4921 tctagatgtt catttataat tttttcaaat tctgcaaaca tggtttgtct aaagacagta
     4981 gttctaaagg attctaaata atggtttatt aaatagactc tttccttttt atccttagta
     5041 ttatttatca tataatctaa tagcaaggcc tcgttagcag tagatgcaac ttcagccagg
     5101 aaaatgctat aattgccata aatgtaaggt tgattttttc tggtgaagta actatggact
     5161 gaatgaccca tttcatgggc tagggtaaat acactgtcta aagtattctg ataattcaac
     5221 agtataaatg gcttagaatc ataggaacca ctggaatatg caccagatct tttcccccta
     5281 ttctcatata catctaccca cctagattta aaaccctttt caactacact taaatattca
     5341 tcttctaaag gctctaagcc cttatataca agctccattc cttcctcgta aggaatatta
     5401 aaatctatgt cttctactat tggagtatac aaatcataca tatgaagctc atctaagcct
     5461 agggcccttt ttcttatttc catatactta tacatgctat ctagattttt gtggacggcc
     5521 tttattaggt tgtcataaac tgataaggga atattgttgg cactaagggc tgcctctctg
     5581 gttgatgaat agttcctcat tctagcatta aatacattgt ttcttaactc tccacttagg
     5641 gttgcggcca aggtgttttc gaatccctta taaaccccat acaggccctg gaaggccgcc
     5701 ttacgaacat tcctatcctt agactccatt aagggtatga agtttccatg agtaatttcc
     5761 acatcttttc cttcttcatc cttaatagtt ggaaacttta aatctgcatc tgttaacata
     5821 ttatatatat tttggggaga catgccaatt aagttaaccc ttgccattat agattcttct
     5881 ctcgaggaaa gcacatgtgg cttctgtctc ataatattat ctaaatgttg tttatataga
     5941 ctcagttcct ggttttcttc aaggaacttt tttaagtctt cctcatctat ggctagtatt
     6001 tctggtacta caaaggacgt cctttcatcc acctctacat acaaattcat ggccctatca
     6061 gataaggatt ggtacttgct ccttctagta tcctcatcta atcttaacct ggcataaacc
     6121 agcacatttt ctactgaacg atatagccta tccctgtctt ttagaacctc caacagggtt
     6181 tggctacttt ctcctaccct gcccttatgg gccacaaatt tttcaactag gccttctacc
     6241 ctcttaaatt ccttttccca ctcttccaca ttggcaaata aggattccac atcccaagta
     6301 tactcttttt ttagatcctc tctttctaaa acctgttttg tcatattatc tcctcctaaa
     6361 tatcaagatt tttataagca taggtcaata tctctcttgc tataggcgca gcaacctggc
     6421 caccggtggc tcctgcctcc tcaataagca cagttactac aaattgaggg tcctcatagg
     6481 gagcaaatcc agtaaaccag ctatgggact ttcctgatgg gttttcggca gttccagttt
     6541 tccctgcaat tttaaattcc tttgatttag catttgtccc tgtgcctgtc tcaactgctt
     6601 ccaacatcat ttctttaacc tgattggcag tagaactgct tatactttgg gttataattt
     6661 cggtctcaaa tttttctata accttatcat tcctgttaat gacctcttta actaagattg
     6721 gcctaaccat ttgcccatta tttgctatag cagatgtaat catagcca
//
LOCUS       DATGSI010000020         4307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_49487, whole genome shotgun sequence.
ACCESSION   DATGSI010000020 DATGSI010000000
VERSION     DATGSI010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49487"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /gene="serS"
                     /locus_tag="VK031_00260"
     CDS             complement(<1..692)
                     /gene="serS"
                     /locus_tag="VK031_00260"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HLS52379.1"
                     /translation="MKEILLEIPNTPHESVVEGLSDEDNVEIRRHGEATAFDFEPKAH
                     WDLGVDLNILDFERASKITGTRFGIFKGLGAQLERALISFMLDCHTLEHGYEEMLTPF
                     MVNRDSMIGTGQLPKFEDDMFHLPGKDYFLIPTAEVPLTNLHRDEILAEKDLPIYYTG
                     YTPCFRKEAGAAGRDTRGLIRNHQFDKVELVKLSKPEDSYDGLEKLTADAETILKKLN
                     LPYRVVSLSSGD"
     gene            complement(723..977)
                     /locus_tag="VK031_00265"
     CDS             complement(723..977)
                     /locus_tag="VK031_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52380.1"
                     /translation="MLDIRRIRKNPDEVKNALKKRNEDFPIDKLLNLDEERRKLLVEV
                     EEMKARQNRVSKEIPKLKKEGKDVSEVVAEMRQLSDKIKD"
     misc_binding    complement(1095..1258)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1550..3532
                     /locus_tag="VK031_00270"
     CDS             1550..3532
                     /locus_tag="VK031_00270"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HLS52381.1"
                     /translation="MKEKFRKKSYNFKNNIILILLILGGIFNDFALRALTVGGAFYWK
                     PIVASIAMIIFISIMALFLSYKNRNYVYGILSIVFSLISGMNYLYYQHYNSFLSFSLV
                     NQLTQVREMGDAIYKTLDFKVLIFAIPPITLIYTIRKLNKIGFFEKIESTRKKTEIVR
                     PLVIGTALLTLVYTTLTSTDLSRLVKQWNRPYIVEQLGIYSFTTADLFKNISSQKLPT
                     FEEDEIVTVLEDLIDENLDQQTENQYTNIFKGRDVYVIHYESAQTFAMDLEFQDGPVT
                     PFLNQMAQEGLFFNNFYPQHSVGTSSDSEFTFSTSLLPINNGTVFMTHANRDYETIQK
                     LLKREGYYVFSMHGNNGDFWNRNIMHNTLGYDRFFSKSDYIIDEEIGLGLSDKSFFRQ
                     SIEKIKEIKEEEDRPIMAKLITLTHHYPFDDVEKYGEFDVGHLEGTDIGNYLKSYHYA
                     DQALESFIEGLDEEGLLENAVVVLYGDHHAKISKADYEKLYNYNEEIDGYYSKDDEEY
                     VAIEGAFQRQLRRTPLIIWSKDQLVNGVVDTPTGMVDVLPTLGNMLGIFNPYQLGNDI
                     MTVENNTVIFPDGSWIDDKSYYSASSTTLYDLATNEEEIVEEPDLIAESQLAEDKIQL
                     SNNIIQSNLIKTFNVLIAKNKVKRPIEITPTEIEAM"
     gene            complement(3599..>4307)
                     /locus_tag="VK031_00275"
     CDS             complement(3599..>4307)
                     /locus_tag="VK031_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HLS52382.1"
                     /translation="GAFIAALAGAEVSKIISGKTDVDIVLVPMVTILVGGVVGLYIGP
                     VMATFMGYIGAFINMATELHPIPMGIIVSVSMGLVLTLPISSAALAISLKLSGLAAGA
                     STVGCAANMIGFAVASFRENGIGGFIAQGIGTSMLQIPNIVKNPRIWLPAIISSAILG
                     PISTHILKMESNAIGAGMGTSGLVGQFAMIDTMGASPKVIIMILFMHLLLPGLISYGV
                     AQWMRKRDYIKPGDMKL"
BASE COUNT         1382 a          799 c          710 g         1416 t
ORIGIN      
        1 aaatctcctg agcttaatga taccacccta taaggtaaat ttaatttttt taagatagtt
       61 tctgcatcag ctgttaattt ttccaatcca tcatatgaat cctccggctt agataattta
      121 actaactcta ccttatcaaa ctgatggttc cttataagcc ctctggtgtc cctaccggct
      181 gctccagctt cttttctaaa gcagggagta tatcctgtgt aatatatggg cagatctttt
      241 tctgctaata tttcatctct atgcaaattt gtcaatggca cctcagcagt aggtatcagg
      301 aaatagtcct tgcctggtaa atgaaacata tcgtcttcaa attttggtag ctgacctgtt
      361 cctatcatac tgtctctatt taccataaag ggggttaaca tttcttcata accatgttct
      421 aaggtatgac aatccagcat aaaactaata agggctcttt ccagttgggc accaagccct
      481 ttgaaaattc caaatctagt ccctgttatt tttgacgccc tttcaaaatc taatatgttt
      541 aaatccacac ccaaatccca gtgggcctta ggctcaaagt caaatgctgt agcttctcca
      601 tgccttctaa tttctacatt gtcctcatca ctaagccctt caactactga ttcatgagga
      661 gtgttaggga tttctagtag tatttccttt atagattcat cagtggttcg aacttcttga
      721 tcttagtcct tgatcttatc agataactgc ctcatttcag ccaccacttc tgatacatct
      781 ttaccttctt tttttaattt tggaatttcc tttgataccc gattttgcct agccttcatc
      841 tcctctactt ctaccagaag ttttcttctt tcttcatcta aatttaatag cttatcaatg
      901 gggaagtcct catttctttt ctttaaagca tttttaactt catctggatt ttttcttatc
      961 cttctaatat ctaacatttc catctcctct tttcattaaa tagttaattt atatttgatt
     1021 cttcccataa ggcctcatct atagaataat tttaatctaa atcccctata aaatcaaaaa
     1081 actctcaatc cccatttagg gacgagagtt aacccgcgtt gccaccctat ttaacctttc
     1141 agttcgctta aaaggtaatg gctccaggat ggattcaata attccaccta ccaattctca
     1201 ccaaccattg gctctctgca aggcttagtt atctactatt tcccttcttt gccacatata
     1261 tatcttgtaa tctcaattat attatatatg aataataagt caagatctgg gcttctttga
     1321 atcttcataa tttaataata ttattattac aatattgtaa ccgaatatta atctaattta
     1381 aagctttatg ctatataata tagtatggtc agattggcca tataaatata tacattctac
     1441 cattatttgg ggtataaaat aaaatgtata taattgattc cataccatat ttattatatg
     1501 gtatatctac atgattctaa agaattatgt acaaaggaag ggatattata tgaaagaaaa
     1561 atttagaaag aagtcttaca attttaaaaa taatataatc ctaatacttt taatattagg
     1621 aggtatcttc aacgattttg ccctaagggc attaactgta ggtggagctt tctattggaa
     1681 gcctattgtg gctagtatag ccatgattat ctttattagt atcatggccc tgtttttatc
     1741 ttataaaaac aggaattatg tttatgggat tttatccata gtatttagtt tgatcagtgg
     1801 tatgaattat ttatattatc aacattataa ttcattttta tccttctctc tggtaaatca
     1861 acttacacag gttagagaaa tgggggatgc aatatataaa actttagatt ttaaggtttt
     1921 aatctttgcc attcctccta ttacattaat atatactatt agaaaactaa ataaaattgg
     1981 tttttttgaa aaaattgaaa gtactaggaa gaaaacagaa atagtaaggc ctttggttat
     2041 aggaacagcc ttattaaccc ttgtatatac tactcttacc agtactgatc ttagccgttt
     2101 ggttaaacaa tggaatagac cctatatagt agagcagtta ggtatatatt catttactac
     2161 tgcagattta tttaaaaata tttcatctca aaaattacca acctttgaag aagatgaaat
     2221 agtaacagtc ttggaggatt taatagatga aaatctagac cagcaaactg aaaatcaata
     2281 caccaacata tttaagggca gggacgtcta tgtaattcac tatgaaagtg cccaaacctt
     2341 tgctatggat ttagagtttc aagatggacc tgttactcca tttttaaatc aaatggccca
     2401 ggagggacta ttctttaata atttctatcc tcaacattca gtagggacaa gtagtgattc
     2461 agaatttacc tttagtacct ccctactacc gattaataat ggtacagtct ttatgaccca
     2521 tgccaataga gattatgaaa ctatacaaaa gcttcttaaa agagaaggct attatgtatt
     2581 tagcatgcat ggaaataatg gtgacttttg gaataggaat ataatgcata ataccttagg
     2641 ttatgataga ttctttagta aaagtgacta tattattgac gaggagattg gtctaggctt
     2701 aagtgataag tccttcttta ggcagtctat agaaaagatt aaagaaatca aggaagaaga
     2761 agataggcct attatggcta aactaatcac cttaacccac cattatccct ttgatgatgt
     2821 ggaaaagtat ggtgaatttg atgttggcca tttagaagga actgatatag gcaattacct
     2881 taaatcctat cactatgcag atcaggcttt ggaaagcttt atagaaggat tggatgagga
     2941 aggtctattg gaaaatgctg tagtggtcct atacggagat caccatgcca aaatatctaa
     3001 ggcagactat gaaaaattat acaattacaa tgaagaaata gatggctatt atagtaaaga
     3061 tgatgaggaa tatgtagcta ttgaaggggc cttccagaga caacttagaa ggacaccttt
     3121 aattatatgg tccaaggatc aacttgtaaa tggagttgta gatactccaa ctggaatggt
     3181 ggatgtatta ccaacccttg gcaatatgtt aggtatattt aatccttacc aattaggtaa
     3241 cgatataatg actgtagaaa acaatacggt aatatttcct gatggcagct ggatagatga
     3301 taaatcctat tactctgctt catctaccac cttgtatgac ctggctacca atgaagagga
     3361 gatagttgaa gagccagatc ttatagctga gagtcaattg gcagaggata aaatccagct
     3421 atctaataat attattcaaa gtaatttgat taagaccttc aatgtattaa tagccaaaaa
     3481 caaggtgaaa agacctattg aaataactcc gaccgaaata gaagctatgt agaagtaatc
     3541 ataaaaaacc ctctaagccc agacagggac ttagagggct ttcttatctt taaatacttt
     3601 acaacttcat atccccaggc ttaatataat ctctcttcct catccactgg gctaccccat
     3661 atgaaattag acctggtagt aaaagatgca taaacaaaat cataattata acctttggtg
     3721 atgctcccat agtatctatc atggcaaatt ggcctactag accgctggtc cccatgccgg
     3781 caccgatagc attactttcc atctttaaaa tatgggtaga tatggggcct aaaatagcag
     3841 atgatataat ggctggtagc catattctgg gattttttac tatattggga atctgaagca
     3901 tggaggtacc tattccctga gctataaaac cgcctattcc attttcccta aaggaggcta
     3961 ctgcaaagcc tatcatattg gctgcgcaac ctacggtact ggcacctgct gctaggccag
     4021 aaagtttaag agagattgcc aaggctgcag agctaatagg cagggttaat acaagtccca
     4081 ttgatactga tacaattata cccataggga tagggtgtag ttcagtggcc atattaataa
     4141 atgcccctat atagcccata aatgtagcca taacaggccc tatataaagg cctacaactc
     4201 ctccaactaa gattgtcacc atagggacta agactatatc aacatcagtt ttgccgctga
     4261 taatttttga cacctctgct cctgccaggg ctgcaataaa ggctcct
//
LOCUS       DATGSI010000021         1055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_49609, whole genome shotgun sequence.
ACCESSION   DATGSI010000021 DATGSI010000000
VERSION     DATGSI010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49609"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VK031_00280"
     CDS             complement(<1..233)
                     /locus_tag="VK031_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLS52383.1"
                     /translation="MVIFIQLSKRQEKIIEIVKTNQPITGEEIANCLNLNRSTLRIDL
                     SILTMFGILDARPRVGYFFTGKTSMGFVADKIK"
     gene            complement(270..>1055)
                     /locus_tag="VK031_00285"
     CDS             complement(270..>1055)
                     /locus_tag="VK031_00285"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HLS52384.1"
                     /translation="FAGDELPSTTAGSILSIADKLDTIAGLFAIGIKPTGSQDPFALR
                     RSALGIINIIIDKRLNLYLNDLIEFALYIYVDEMGLAFNYDRVKKEIRDFFMGRIKNM
                     FADKKVRYDIIEAVLNTDTDNIYDMKIRVDKISQWLEKEDLTEILTAFNRVASLAKEA
                     ESNEVTRNLLTEDETELYNAFNKIEDRVIELIDEKEYDKALDLLATLREPIDKFFDNV
                     MVMVDDEEIRANRLGLLKKIYDIMIQVCDLSKIVNNTSKDKTN"
BASE COUNT          299 a          222 c          144 g          390 t
ORIGIN      
        1 ttttttattt tatctgccac aaagcccata ctggtcttac cagtaaaaaa gtatcctacc
       61 ctaggtcttg catccagtat gccaaacata gttaaaatag atagatcaat ccttaaggtt
      121 gacctattta agtttaagca gtttgctatt tcctctccag taatcggttg gttagttttt
      181 acaatttcaa taattttttc ctgtcttttg ctaagttgta taaatatcac cacctaaggc
      241 cccttaaggc cggcaactta gagattagcc tagttagttt tgtctttgct tgtattatta
      301 acaatctttg acaaatcaca tacctgaatc attatatcat agattttctt aagcaggcct
      361 aacctattgg ccctgatttc ttcgtcatct accataacca tcacattgtc aaagaacttg
      421 tctataggtt ctcttaatgt ggctaacaaa tctaaagcct tatcatattc tttttcatct
      481 attagttcta ttaccctatc ttctatcttg ttaaaggcat tataaagctc tgtttcatct
      541 tctgtcagta aatttcttgt tacttcattt gattcagctt ctttagccag gctagcaacc
      601 ctattaaagg cagttaatat ttcagttaaa tcttcttttt caagccactg gctgatttta
      661 tcaaccctaa ttttcatatc ataaatgttg tcagtatcag tgtttagcac tgcctctatt
      721 atatcatacc tgactttttt atcggcaaac atgtttttta ttcgccccat aaagaagtct
      781 ctaatttcct tcttaaccct atcataatta aatgcaagcc ccatttcatc cacataaata
      841 tatagggcaa attctattag gtcatttaag tataagttta acctcttatc aatgattata
      901 tttataatcc ctagagcact tcttcttaag gcaaatggat cctgagagcc tgtgggttta
      961 atcccaatgg caaacaagcc ggctatggta tctagtttgt cagcaatact caaaattgag
     1021 cctgcagtag tactgggtag ctcatctcct gcaaa
//
LOCUS       DATGSI010000022         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_474769, whole genome shotgun sequence.
ACCESSION   DATGSI010000022 DATGSI010000000
VERSION     DATGSI010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474769"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VK031_00290"
     CDS             <1..799
                     /locus_tag="VK031_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLS52385.1"
                     /translation="DKHYSRKENLIFPYLEKYKITAPPKVMWGVHDEIRAKIKGVILD
                     LEKYTGKKEELIQKAKDSIHQVEEMIFKEENILFPMSLETLVEDEWITIYNESGEIGY
                     TFVEPKTEWADKRIERIEEIKKEEIGFEDGLINFDYGSLSGEEINAIFEVLPGELSFV
                     DKDDRVKFFTKGEDRIFARTKSVIGRPVELCHPHSSVHKVNQILEDFKSGKKDSEDFW
                     IHFRDMYVLIRFFAVRNKDGEYLGTLEYVQNIKPMQELTGEKRLLSE"
     gene            961..1974
                     /gene="fni"
                     /locus_tag="VK031_00295"
     CDS             961..1974
                     /gene="fni"
                     /locus_tag="VK031_00295"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HLS52386.1"
                     /translation="MRKQRKREHVENYLKTSYEGDNLFNNIFLQHNALPELNLIDIDT
                     GTEFLGKEISYPIIINAMTGGTEFTRDINKDLSLLAREFNIPMAVGSEDILLCENDAC
                     RDSFRIVRENVGPEGVVMANLNGLASLDDVKEAIDVIEADAIQIRLNPAQELARTEGE
                     REFTGILKNIENIVQNIDKPVIVKEVGFGISKDVAERLYNVGVRYIDVSGHGGTNFIE
                     IENLRNINMDLGELYSWGVPTALSLIKCRELPDDLKLIASGGIRTSLDIVKSIILGAK
                     VVGISGEILRYLVHGGYDNTKRYLDETVYKLKMIMLLLGKRNMEELKDTDYKIYGKLK
                     ELI"
     gene            complement(2063..>2865)
                     /locus_tag="VK031_00300"
     CDS             complement(2063..>2865)
                     /locus_tag="VK031_00300"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52387.1"
                     /translation="LEIEEIKVEKKKRWLKDNNEKEFLFIRKDKVGPLLEHEAHLLNW
                     LFNRIGNGQKVDTYQIKKYRERNGISFYKDYNKWNKLVDRQLKSRDYDEPRRTAPAFV
                     LISFGAILLVFSILALLYNATYAIFTLVTGAACLIYGFILAMRKSDKGYLQKKLWEEF
                     KKDIGKYEGEIGITTDKSLIYAIALGLPMKELEDYRSSIDTGYYPMYWGYWFFYTNRR
                     GGSIIEDRINHGFYGHEGTSTSTSTFGGGGGFTGGGGGGAGGGGTGGF"
BASE COUNT         1019 a          452 c          544 g          850 t
ORIGIN      
        1 agataaacac tacagcagga aagaaaatct aatttttcct tatttagaaa agtataaaat
       61 tacagcaccg cctaaggtta tgtggggagt tcatgatgaa atcagggcta aaatcaaggg
      121 ggtaatcctg gacttagaaa agtatacagg aaaaaaagaa gagctaatcc aaaaagctaa
      181 agatagtata caccaggtgg aggagatgat ctttaaagaa gaaaatatcc tatttcccat
      241 gagcctggaa accctggtag aagatgaatg gataaccata tataatgaat ctggtgaaat
      301 aggttatacc tttgtagaac ctaagacaga atgggcagat aaaagaatag aaagaataga
      361 agaaattaaa aaggaagaaa tagggtttga agatgggctt ataaactttg actatggctc
      421 cctaagtgga gaagagatta atgctatttt tgaagtctta cctggagaac tttcctttgt
      481 agataaagat gatagggtaa aattctttac caagggtgaa gataggatat ttgccagaac
      541 caagtcagta ataggtagac cagtggagct ttgccatccc cattcctctg tccacaaggt
      601 aaatcaaatc ttagaagact ttaagtctgg caaaaaagat tcagaagatt tttggataca
      661 ttttagagat atgtatgtgc ttattagatt ctttgccgta agaaataagg atggagaata
      721 tttaggaact ttagaatatg tacaaaacat taaaccaatg caagaattaa caggtgagaa
      781 aagattatta agtgaatagc tgctacaaat acccatataa gagattatat gggtatttta
      841 catttaaagg atagggaata atgtctaaaa gtaagttttt taattgtaat taaatctttc
      901 ttgtagtatt atatgagttg tatatatctt ttatcatgcc ataattaggg gtgaaataaa
      961 gtgaggaagc aaaggaaaag ggaacatgtg gaaaactatc tgaaaaccag ctatgaagga
     1021 gataatcttt tcaacaatat attcttgcag cataatgcat taccagaact aaatttaatt
     1081 gatatagata ctggaacaga atttttaggc aaagaaatct cgtatcctat tattataaat
     1141 gctatgactg gtggcactga atttacccgt gatataaata aagacttatc attattggca
     1201 agagaattta atattcctat ggctgtaggg tcagaagata tacttttatg tgaaaatgat
     1261 gcctgtaggg attcctttcg aatagttaga gaaaatgtag gtccagaagg tgtagtaatg
     1321 gcaaatttaa atggactggc cagcctagat gatgtaaaag aggcgataga tgtaatagag
     1381 gcagatgcta tacaaattcg cctgaatcct gcccaggaat tggctagaac agaaggagaa
     1441 agggaattta ctggcatact gaaaaatata gaaaacattg ttcaaaatat agacaagcca
     1501 gttatagtaa aggaagttgg ttttggtata tctaaggatg tggctgaaag gctttataat
     1561 gtgggagtta gatatattga tgtttcaggc catggaggaa ctaattttat cgaaattgaa
     1621 aacctgagaa atattaatat ggatctaggt gaactatatt cctggggtgt tcctactgcc
     1681 ctgtccttaa taaaatgcag ggaattacca gatgatttaa aacttatagc cagtggagga
     1741 attcgaacaa gcctagatat agtaaagtct attatactag gtgccaaggt tgtgggcatt
     1801 agcggagaaa ttctaagata tctagtccat ggtggatatg acaatactaa aagatatttg
     1861 gatgaaacag tttacaaact aaagatgatt atgttgctcc taggcaagag aaatatggaa
     1921 gaattaaaag atacagacta taaaatttat gggaaattaa aagagttgat ttaaagaaag
     1981 actgcctctc ctattaacag gagaggcagt cttatattca gattaagctt tggcgcctag
     2041 atagaaagga tcagcctcca aattaaaatc ctccagtacc gccaccaccg gcaccgcctc
     2101 cgccaccacc agtaaatcct cctccaccac caaaggtaga tgtggaagta gaagtaccct
     2161 catgtccata gaagccatga tttattctat cttctataat agagccccct cttctattgg
     2221 tataaaagaa ccagtaaccc caatacatag gataatatcc tgtatcaatg gaacttctat
     2281 aatcctcaag ttccttcata ggtagcccta gggctatagc atatattaag gatttatcag
     2341 tagttatacc aatttcacct tcatatttac caatatcctt tttaaattcc tcccacaatt
     2401 ttttctgtag ataaccttta tctgactttc tcatggccaa tataaaccca taaatgaggc
     2461 aggctgctcc agttactagg gtaaatatgg cataagtagc attatatagt agggccagaa
     2521 tactaaatac aaggagtata gccccaaagg atattaaaac aaaggcaggt gcagtccttc
     2581 taggctcatc atagtcccta cttttcaatt gcctatctac caacttattc cacttattat
     2641 agtctttata aaaacttatc ccatttcttt ccctgtattt ctttatttga taagtgtcca
     2701 ctttttgccc attgccaatt ctattaaaaa gccagttaag tagatgagct tcatgttcta
     2761 ataaaggacc aactttatcc tttcttataa ataaaaattc cttttcatta ttatctttaa
     2821 gccatctttt ctttttttct accttaattt cctcaatttc aagga
//
LOCUS       DATGSI010000023         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_474859, whole genome shotgun sequence.
ACCESSION   DATGSI010000023 DATGSI010000000
VERSION     DATGSI010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474859"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VK031_00305"
     CDS             complement(<1..464)
                     /locus_tag="VK031_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLS52388.1"
                     /translation="MNNKKYAYPYILWLLIFILIPLILVLYYAFTVGDGQTFSEFVFS
                     LDNFTRFFTPTYLKVLTRSIRLALISTIICLILGYPLAYIISKERVRRRNVMILSLVI
                     PMWMNFLLRTYAWLTLLGRNGFINNLLAKLGLGPLNLMYNNKAVLLGMVYNFL"
     gene            complement(457..1500)
                     /gene="potA"
                     /locus_tag="VK031_00310"
     CDS             complement(457..1500)
                     /gene="potA"
                     /locus_tag="VK031_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HLS52389.1"
                     /translation="MEIIKLENIRKSFGDLTVLNNINLSIKKNEFLTLLGPSGCGKTT
                     SLRIIGGFEQPTSGRLIFEDRDITNLPAYKRQINTVFQKYALFPHMNVYENIAFGLRI
                     KKLSNDVIEKKVKDILKLVNLIGFENRSIDSLSGGQQQRIAIARALVNEPEVLLLDEP
                     LGALDLKLRKDMQIELKNMQQRLGITFIYVTHDQEEALTMSDTIAVMNEGRIQQIGSP
                     VDIYNEPENAFVASFIGESNIIDGIMHKDYLVEFANKKFNCVDKGFEKCEKVDVVIRP
                     EDIEIVEKEAGQWTGIVQSVIFKGVHYEMMVKSEGFIWMIHSTIMKAEGTEIGMRVSP
                     ENIHVMKKVEADE"
     gene            complement(1515..2054)
                     /locus_tag="VK031_00315"
     CDS             complement(1515..2054)
                     /locus_tag="VK031_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HLS52390.1"
                     /translation="MKIGEKIRRLRIKGSLTQEELAERCELTKGFISQVERDLTSPSI
                     ATLVDILEGLGTNLKDFFNETVDEKIVFTKEDIFESQNEDLKYSLKWIIPNAQKNLME
                     PILIEMEPGGKTKVDSPHEGEEFGHIINGSIFLHIGQEKLKVRKGESFYFKANSNHYI
                     SNAGKTRARLIWVSTPPSF"
     gene            complement(2211..>2837)
                     /locus_tag="VK031_00320"
     CDS             complement(2211..>2837)
                     /locus_tag="VK031_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967097.1"
                     /note="Required for the synthesis of the thiazole moiety;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HLS52391.1"
                     /translation="LLLLSGGIDSPVAGFMMAKRGLAIDCVHYHSYPFTSERAEEKVK
                     TLAKILSRYVGKINFYSVNILEIQKAINRNCPEREMTILSRRFMMRIAEAIARKNKHQ
                     ALITGESLGQVASQTIEGIGATNAAVNLPVLRPLVGMDKVDIVDISKDIETYETSILP
                     YEDCCTVFLPKHPVTKPRLEDIESSEAALEIEDLVETAVENMKVYTIE"
BASE COUNT          795 a          584 c          433 g         1025 t
ORIGIN      
        1 agaaaattat ataccatacc taacaggact gccttattgt tgtacataag gtttaaaggg
       61 cctagtccta atttggccaa taggttattt ataaagccat tcctgcctaa aagggttagc
      121 caggcatagg tccttaaaag aaagttcatc cacattggta taactagact taaaatcatt
      181 acatttcttc ttctaaccct ttccttggaa ataatatagg ccaagggata acctaggatg
      241 agacatatta tggttgagat tagggccagc ctaattgacc tggttaaaac ctttaaatag
      301 gtaggagtaa aaaacctggt aaagttatct aaggagaaga caaattcaga aaaagtctgg
      361 ccatctccca cagtaaaggc atagtagaga actaatataa ggggaattag aataaaaatt
      421 aacagccata atatataagg atatgcatat tttttgttat tcatcagctt ccaccttctt
      481 catcacatgg atattttctg ggcttactct catacctatt tctgtacctt ctgctttcat
      541 aatggtgctg tggatcatcc aaataaagcc ctcagatttt accatcattt cataatggac
      601 tcccttaaag ataactgact gaactattcc agtccactgg ccagcctcct tttccactat
      661 ttctatatct tctggcctaa ttaccacatc taccttttca cacttttcaa agcctttgtc
      721 tacacagttg aatttcttat tggcaaattc tactaaataa tctttatgca taattccatc
      781 tattatattg ctctctccta taaaactggc tacaaaggca ttttccggct cgttgtaaat
      841 atctactggg ctacctattt gttggattct accttcattc ataacggcta ttgtatctga
      901 catggttaaa gcctcttctt gatcatgggt tacatagata aaggtaattc ctaatctttg
      961 ttgcatgttt tttagttcaa tctgcatgtc ttttcttaac ttcaggtcta aggctcccaa
     1021 aggctcatct aatagtaaga cttcaggttc attaaccaag gccctggcta tggcaattct
     1081 ttgttgttga ccaccgctta gagaatcaat agatctgttt tcaaagccta ttaggttgac
     1141 taactttaat atatctttaa ccttcttttc aattacatca tttgataatt tcttaattct
     1201 taggccaaag gcaatatttt cataaacatt catatgggga aaaagggcgt atttttgaaa
     1261 gactgtattt atttgcctct tataggctgg tagatttgtt atgtccctgt cttcaaaaat
     1321 tagtctacct gatgttggtt gttcaaaacc acctattatc ctaagactgg tggttttgcc
     1381 acatccactg ggccccaata gggttaggaa ttcattcttt tttatagata aatttatatt
     1441 atttagaaca gttaaatctc caaagctttt tctaatattt tccagcttaa ttatctccat
     1501 ctttcatctc ctccttaaaa gctaggcggt gtactaaccc aaatgagcct agccctagtc
     1561 tttcctgcat tgcttatata atggtttgag ttggccttaa aataaaagct ttctcccttt
     1621 cttaccttta atttttcctg ccctatatgc aaaaatatgc tgccatttat aatatggcca
     1681 aattcttccc cttcatgggg ggaatctact ttagtctttc ctcccggctc catctctata
     1741 agaattggtt ccattagatt tttttgtgca ttgggaatta tccattttaa actgtatttt
     1801 aagtcctcat tttggctttc aaaaatatct tccttggtaa atactatctt ttcatctact
     1861 gtttcattaa aaaagtcttt taagttagtt cccagacctt ccaaaatatc caccaaggtg
     1921 gctatggaag gagaggttag atctctttct acctgggata taaatccttt tgttaactca
     1981 cacctctctg ctaattcctc ttgggttaat gatcctttaa tccttagtct acggattttt
     2041 tctccaattt tcataaatac cacctgtctt tcagtatata ttaagtaaaa tgtttattta
     2101 tactaaactt ttataactac catattataa tttattcttt ccatatatgt caatagttta
     2161 tttaaaatct ccacaaaaaa acagcctgac ctgtcaggct gttttaagtc ttattcaatt
     2221 gtatatacct tcatattttc cactgctgtc tcaaccaagt cttctatctc taaagctgct
     2281 tcagagcttt ctatgtcctc taatcttggc ttggttactg gatgctttgg taaaaagact
     2341 gtacaacagt cttcgtaggg caggatggag gtttcataag tttctatatc cttagaaata
     2401 tctactatat ccaccttatc catacctacc aagggcctta aaactggtaa atttactgct
     2461 gcattggtag cccctatgcc ctcaatggtt tggctggcta cttgtcctaa actttctcca
     2521 gtaattaggg cctggtgttt attttttctg gctatggcct cagcaatcct catcataaac
     2581 cttctagaca ggatggtcat ttccctttct ggacaatttc tgtttatggc cttttgtatt
     2641 tctaaaatat ttacactata gaagttaatt ttacctacat atctggataa aatcttggct
     2701 aaagtcttta ccttttcttc tgccctttct gatgtgaagg gataagaatg ataatggaca
     2761 cagtctatgg ccaggcctct tttggccatc ataaagccgg ctactggact atcaatacct
     2821 cctgatagaa ggagtag
//
LOCUS       DATGSI010000024         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_521508, whole genome shotgun sequence.
ACCESSION   DATGSI010000024 DATGSI010000000
VERSION     DATGSI010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521508"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VK031_00325"
     CDS             complement(<1..544)
                     /locus_tag="VK031_00325"
                     /inference="COORDINATES: protein motif:HMM:NF023495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HLS52392.1"
                     /translation="MILSSVIGIIVGYYIRRYIGEGKIKNAEELASRIIEEAKRDGET
                     SKKELLLEAKEEIHKLRTEAERENRERRNEIQKLERRVLKKEENLDRKSDSLERREDA
                     ISRKTRYLEEKESSIEELYKKQQAELERLSGLTSEEAKELLLNDIKKEISRESAIMTK
                     EIESQAKQEADKKAREILSTA"
     gene            complement(733..1386)
                     /locus_tag="VK031_00330"
     CDS             complement(733..1386)
                     /locus_tag="VK031_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HLS52393.1"
                     /translation="MMDDISKLQENLDILKDRIAKERGKKEKLLEQLATSKEQAEELE
                     KKLDLLDQVIVLFQKTSEFAREQAKAQIESLVTKCLQFIFDSDVKFQVEIKELRNKAN
                     AEFYVVTETEDMIIKTKPELSRGGGVVDIVSLALRIAFLEIHKPRITGPLILDEPAKH
                     VSEEYIFNVGNFLKQSSIMFNRQIIMITHDQHLAAIGDKGYRIALDGTVSTLSQIEN"
     gene            complement(1391..1639)
                     /locus_tag="VK031_00335"
     CDS             complement(1391..1639)
                     /locus_tag="VK031_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52394.1"
                     /translation="MNYEKQLSSLKENIEKAKSIKYRAEARLEQLKKQEEDIINELKK
                     LDVEPEELDKKIEELTDEIDELFEEANKLLPKELLEKM"
     gene            complement(1658..3103)
                     /locus_tag="VK031_00340"
     CDS             complement(1658..3103)
                     /locus_tag="VK031_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLS52395.1"
                     /translation="MKDLKKVILNNFQSHKHTVVELDPGLNVIVGPSDSGKSAIIRGI
                     KWALYNEPAGDFFIREGESECSVTLIFADKTKLIRKRSRSKNIYELVKSDGEKIRFEG
                     FGTSVPEEIVEEIGITKIYLDSDSSNAINLGEQLEGPFLLSEKASVRASAIGRIVGVN
                     IIDDALRVTIRDRTNASRFKRDLEERIESIEEELKEYDYLDGLSEKVSRAEKLINIII
                     DKRQTLHRLKKLSDMYNRTKVSIKQLEKQLMKLKDLDRAESLQLQLENLNKDRKYYTK
                     QDQSLKENKLAIEKNQDLLEQLKFLEQVNKLHQKAFEVINLRTNIIQQSGRLKDTKGK
                     IKETEELINKLSPLPGLDRRLDRSQNGVELLDKLSQLKARLRDCQERIKKGKAYLCKF
                     KGTDHAEQISENVREKAVTIKLLKDYKKQLAQVNSGIRKKKDCIKSSDNQIKLELKKY
                     KELLSKTELCPFCLNEITREHIDHIMTHITE"
     gene            complement(3103..>3511)
                     /locus_tag="VK031_00345"
     CDS             complement(3103..>3511)
                     /locus_tag="VK031_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLS52396.1"
                     /translation="GHYHSGFKHVKIEGKHFINPGSMVRISNSIQELERRPKVVIIDL
                     VDGIDISYKYLQSAEAGERVLDRKEIEASMYKQERIYEFKQNIDAAMDFDKIDINDLL
                     IEVSNAEDLEEDVKEEALRRIAEVQMNKAHGDI"
BASE COUNT          887 a          753 c          434 g         1437 t
ORIGIN      
        1 ttgctgtaga taaaatttcc ctagcctttt tatcagcctc ttgttttgct tgagattcaa
       61 tttccttagt cataatggct gattcacggg aaatctcctt cttaatatcg tttagtaaca
      121 gttcttttgc ttcttcagaa gttaaaccag atagtctttc cagctcagcc tgctgctttt
      181 tatacaattc ctcaattgag gattcctttt cttcaagata tctagttttc ctagatatgg
      241 cgtcttctct tctttctagt gaatcagatt ttctatctag gttctcttct ttctttaaga
      301 cccttctttc caacttttgt atctcatttc gtctttctct gttttctctt tcagcctctg
      361 ttcttaactt atggatctct tccttggctt caagaagaag ttcctttttg ctggtttctc
      421 catcacgctt ggcttcttct ataatcctgc ttgctaattc ttcagcattc tttattttcc
      481 cttcgcctat atatctcctt atatagtaac caacgattat tccaataaca gagctaagaa
      541 tcaaatataa agcttcaata gttcaacacc tccgtttgta tatagttttc aagttccata
      601 ctacattcat aatttcataa attacaaatt aagtcgtaaa atatttaatc ctaattgcaa
      661 aggattgtct atattattgt atggttacac aactttattt tattacttat ctaatctaat
      721 gtcaagaatt aattagtttt caatctggct taaggtgctg actgtaccat ctaaagctat
      781 tctataacct ttatctccaa ttgctgctaa atgctggtca tgggttatca taataatctg
      841 tctattaaac attatggagc tttgttttaa aaagtttccc acattaaaga tatactcttc
      901 acttacatgt ttagcaggct catcaagtat caatggccct gtaattctag gtttatgtat
      961 ctctaggaag gcaatcctta atgctagaga tactatatca acaactccac cacccctaga
     1021 tagttcaggc ttagttttaa ttatcatatc ttctgtttcg gtaacaacat aaaattcagc
     1081 attggcctta ttccgcaatt ccttaatttc tacttggaat tttacatctg agtcaaatat
     1141 aaactgtaaa catttagtaa ccaggctttc tatttgggcc ttagcttgct ccctagcaaa
     1201 ttctgatgtt ttttggaata atacaataac ctgatccagt aggtccaatt ttttctccaa
     1261 ttcctctgcc tgttctttgc tggtggctaa ttgttccaat aatttttctt ttttccctct
     1321 ttctttagct atcctatctt ttagaatatc taggttttcc tgtaatttgg aaatatcatc
     1381 catcatctta tcacatcttt tctaagagtt cttttggcaa taatttatta gcttcctcaa
     1441 ataactcatc tatttcatcg gttaattctt ctattttttt atctaattct tctggttcca
     1501 catctaattt ctttagctca ttaattatat cttcctcttg tttctttagt tgctctaacc
     1561 tggcttccgc cctatatttt attgatttag ccttttcaat attttctttt aatgaactta
     1621 actgcttttc ataattcatt atcctaacct ccctgattta ttcagttata tgggtcataa
     1681 tatggtctat atgctcccta gttatttcat ttaggcagaa agggcacaat tctgtctttg
     1741 atagtagttc tttatatttt ttcaattcca actttatttg gttatcagag ctttttatac
     1801 aatccttttt cttccttata ccgctgttta cctgcgccag ttgttttttg taatccttta
     1861 aaagttttat agttacagcc ttctctctca cattttctga tatctgctct gcatgatctg
     1921 tgcctttaaa tttgcaaaga tacgccttac cttttttaat tctttcttgg caatccctta
     1981 atctggcctt taactggctt aatttatcca ataactctac cccattttga gatctgtcca
     2041 gccttcgatc taagcctggt aggggcgaca atttatttat taattcttcc gtttctttta
     2101 tttttccttt agtatccttc aatctacctg actgttggat aatattggtc cttaagttta
     2161 tcacttcaaa ggctttctgg tgtaatttat taacctgttc taggaatttt aactgttcta
     2221 gtaaatcttg gtttttttct atggctagtt tgttttcctt taaactttgg tcttgcttgg
     2281 tgtagtattt acgatcctta tttaaatttt ccagttgtag ctgaaggctt tccgccctat
     2341 ctaggtcttt aagcttcatt aactgttttt ctagctgctt aattgatact ttggttctat
     2401 tgtacatatc agatagtttc ttaagcctat gaagggtttg ccttttatct ataataatat
     2461 tgataagttt ttctgcccta cttacctttt cagacaagcc atcaagatag tcatattctt
     2521 taagctcttc ttctattgat tcaattcttt cttctaaatc tcttttaaat ctggatgcat
     2581 tggtacgatc tcttattgta actctaaggg cgtcatctat aatattaacc cctactatcc
     2641 tccctatggc actggccctg acactggctt tttctgaaag caggaatggc ccttccaact
     2701 gttctccaag gtttatcgcg ttagaactat cactatctag ataaattttt gtaattccaa
     2761 tttcttcaac tatttcctca ggcacactgg ttccaaatcc ttcaaatcta attttttctc
     2821 catcactttt caccagctca taaatatttt tagatctact tctcttcctt attaacttag
     2881 tcttatcagc aaatattagg gttaccgagc attcactttc tccttctctt ataaagaaat
     2941 ccccagctgg ctcattatat aaagcccatt taatccctct aattatagca gactttccac
     3001 tgtctgatgg gccaactatt acatttaagc caggatccag ctcaacaaca gtatgcttat
     3061 gggattgaaa attatttaat ataacttttt ttaagtcttt cattatatat caccatgcgc
     3121 tttgttcatt tggacttcag caatccttct taaagcctct tcctttacat cttcttctaa
     3181 atcttctgca ttagacacct ctataagtaa atcattaatg tctattttat caaaatccat
     3241 agctgcatca atattttgct taaactcata tattcgctcc tgtttataca tactggcctc
     3301 aatttctttc ctatccaata ctcgttcgcc tgcttctgca ctttgtaaat atttataact
     3361 aatatctatc ccatcaacca ggtcaataat caccacttta ggccttcttt ccaactcctg
     3421 tatactgtta gaaatccgaa ccatgctacc gggattaata aaatgctttc cttcaatctt
     3481 aacatgctta aatcctgaat gataatgacc a
//
LOCUS       DATGSI010000025         1284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_50082, whole genome shotgun sequence.
ACCESSION   DATGSI010000025 DATGSI010000000
VERSION     DATGSI010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50082"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VK031_00350"
     CDS             complement(<1..252)
                     /locus_tag="VK031_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52397.1"
                     /translation="MHFLKTIIPLLVLSSLIGMSSLHAQSYEKPSNLLKRSQLKGNID
                     ILGANISYEKRIGNGLTAYFDGGLTYGFIINGGLLYEGKQ"
     gene            complement(277..>1284)
                     /locus_tag="VK031_00355"
     CDS             complement(277..>1284)
                     /locus_tag="VK031_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52398.1"
                     /translation="IFKPEKENRVYNILDSLRRVKYKLSNQNRKIPGEVKKGQYLYFV
                     ESGIYRFDTFKNEHTEEAKKNTQYYYARETTDDPLPQSIYDQFITFGYDSHTFAGQTL
                     QGIFGRNKSHYNKFDRKHRVKVKLYNLNYFVYASVGIKVKMHKRGWTGIWHTVKANEI
                     RLGWDGLIVDIEIPYAPALPHSMQINANTTKLGTITFLSKNFSIAGVSINNDLVNKAL
                     NGALKSGFKSAVGTIWDYVRKSVAPSQYETEENEVRAFRAIYPNKARIVFDRYEESKK
                     NVKKIVEVFDTNVGIKFTWDGSGLPNPSPNTYKYTLVGGSVYGAAKYNGTWKGVRVKK
                     Y"
BASE COUNT          381 a          267 c          173 g          463 t
ORIGIN      
        1 ctgtttccct tcatagagaa gccctccatt tataataaac ccataggtca accctccatc
       61 gaaataagcc gttagcccgt tcccaattct tttttcataa cttatattag ctccaagtat
      121 atcaatattc ccctttaatt ggcttctttt aagaaggtta ctaggctttt catagctttg
      181 agcatgaaga gaactcattc caatgagaga ggataatact aatagaggaa ttattgtctt
      241 taaaaaatgc atattatagt ctcttaattt ataacattag tatttcttca ctcggacacc
      301 cttccaagtt ccgttatatt tagctgcacc atatacacta ccgccaacta atgtatattt
      361 ataagtattg gggctagggt taggaagtcc acttccatcc caagtaaact taatacctac
      421 attggtatca aatacttcaa ctattttctt gacatttttt ttgctttctt catacctatc
      481 gaatactatc cttgccttat taggataaat tgcgcgaaaa gcccttactt cgttttcttc
      541 tgtttcatat tgacttggcg caacactttt acgtacataa tcccaaattg ttccaactgc
      601 agacttaaaa ccacttttaa gagctccgtt taaagcttta tttaccaaat cattattaat
      661 tgaaacaccc gcaatcgaga aattcttgga taaaaatgtt atagtaccca acttcgttgt
      721 gttagcatta atttgcatag aatgtgggag agcaggagca taaggtattt ctatatctac
      781 tattaaccca tcccatccca gtcttatttc gttcgctttt acagtatgcc atatcccggt
      841 ccatcctcgt ttatgcatct ttaccttaat tccaacagat gcataaacaa aataattaag
      901 attatatagt tttactttta ctctatgctt ccgatcaaat ttattataat gagatttatt
      961 cctcccaaaa ataccttgaa gagtttgtcc tgcaaaagta tgagagtcat aaccgaaagt
     1021 tataaactgg tcataaatac tctgaggtaa aggatcatct gttgtttctc tggcataata
     1081 gtactgtgta ttctttttag cttcttccgt atgctcattt ttaaacgtat cgaaacgata
     1141 aatacctgac tctacaaagt aaagatattg tcccttttta acttcgcccg gtattttcct
     1201 attctggtta gacagcttgt attttaccct tctaaggcta tccaatatat tatacactct
     1261 attttctttt tcaggcttaa atat
//
LOCUS       DATGSI010000026         1192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_616168, whole genome shotgun sequence.
ACCESSION   DATGSI010000026 DATGSI010000000
VERSION     DATGSI010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616168"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VK031_00360"
     CDS             <1..927
                     /locus_tag="VK031_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HLS52399.1"
                     /translation="PEAEYYPLVNRLKGQVIKISPTSPDYVNPMDINLNYSEDENPLS
                     LKSDFILSLCELIVGGKDGLKPVEKSIIDRCVRLIYADYLADPVAEKMPILGDLYDAL
                     ENQEEKEAQHIRAALEIYVTGSLNLFNHRTNVDITNRLVCFDIKELGKQLKKLGMLVV
                     QDQVWNRVTVNRAEKRSTRYYMDEFHLLLKEEQTAAYSVEIWKRFRKWGGIPTGITQN
                     VKDLLSSREVENIFENSDFIYMLNQASGDRQILAKQLNISPHQLSYVTHSGEGEGLLF
                     YGNVILPFTDKFPKDTELYSIMTTKPNEINEE"
     gene            938..>1192
                     /locus_tag="VK031_00365"
     CDS             938..>1192
                     /locus_tag="VK031_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HLS52400.1"
                     /translation="MSKTSKKPFRPAGKVSRLTFTEEERADPNLEKPIKKAEKAGDKL
                     EKAQDKIPKKRSLVSKRTVDAKTGKAKVHLYFEESDKPKPP"
BASE COUNT          428 a          205 c          248 g          311 t
ORIGIN      
        1 ccggaagcgg agtattatcc ccttgtgaac cgactaaagg ggcaagtaat taagatttcg
       61 cctacttccc ctgactatgt aaatcctatg gatattaatc ttaattattc tgaagatgaa
      121 aaccctttat ccctaaaatc tgattttatc ctatccttat gtgagttgat tgtagggggt
      181 aaggatggtt taaagcctgt agaaaaaagt atcatagata ggtgtgtacg gttaatttat
      241 gcagattatt tagcagaccc agtagctgag aaaatgccta tattgggaga tttatatgat
      301 gcactagaaa atcaagagga aaaagaggca cagcatatta gagctgctct tgaaatttat
      361 gtaacaggct ccctgaatct ctttaatcac cgtacaaatg tagatataac caatcgcctt
      421 gtttgctttg atattaagga attagggaaa caattaaaga aattaggaat gttggtagtc
      481 caagaccaag tatggaacag ggtaacagta aaccgtgcag aaaagcggtc aactaggtac
      541 tatatggatg agttccacct actactaaag gaggaacaga cagcagccta tagcgtagag
      601 atttggaaaa ggtttagaaa atggggcggt attcctacag gtattaccca aaatgtaaaa
      661 gacctattat cttcccgtga ggtagaaaat atctttgaaa actccgattt tatctatatg
      721 ctcaatcaag catcagggga taggcaaatc cttgccaaac aacttaatat ttcgccccat
      781 caattgtcct atgtaactca ttcaggtgag ggagaaggac ttttgtttta tggaaatgta
      841 atactaccct ttactgataa gtttccaaag gatacagagt tatacagcat tatgactact
      901 aagccaaacg aaattaacga ggaataggag gtgttccatg agcaaaacaa gcaaaaagcc
      961 cttccgccct gctggtaagg tatcaagact tacctttaca gaagaagaaa gggctgatcc
     1021 taatcttgaa aaaccaataa agaaagcaga aaaagcaggg gataaattag aaaaggcaca
     1081 ggataaaata ccaaaaaaga gaagtttagt tagcaaacgc accgttgatg ccaagacagg
     1141 caaagcaaag gtgcatttat attttgaaga aagtgataaa cctaagccac ct
//
LOCUS       DATGSI010000027         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_144538, whole genome shotgun sequence.
ACCESSION   DATGSI010000027 DATGSI010000000
VERSION     DATGSI010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144538"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VK031_00370"
     CDS             complement(<1..811)
                     /locus_tag="VK031_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HLS52401.1"
                     /translation="MKIKTSQDLYEYHDKFKENLKNQRKQIVVCAGTGCVAGGALKVY
                     DEFKRLIEEKGLGNKAKLIKEVEDIKLKKSGCPGLCEAGPLVIIEPGDILYTKVKVEH
                     CKDIIHETFLKDNPIDELLFTQGEEVFTSKHSIPFYINQNRLVLKDCGEIDPESIEEY
                     IGNGGYLALAKVLNEMSPKEVCDEIVESNLRGRGGGGFPAGIKWSQVLSYESDMKYVI
                     CNGDEGDPGAFMDMCIMEGIPHAVIEGMIIAAYATKAKEGYIYVRAEYPLAV"
     gene            complement(801..>1050)
                     /locus_tag="VK031_00375"
     CDS             complement(801..>1050)
                     /locus_tag="VK031_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HLS52402.1"
                     /translation="DGTACHIRKSQPILDALRKELGIPEGKSTTEDLLFTVEMVSCLG
                     ACSLAPVVNVDGVVYAKMTPNKATELVRKIREGSLNED"
BASE COUNT          292 a          253 c          141 g          364 t
ORIGIN      
        1 ttacagccaa aggatattct gccctaacat agatatatcc ttccttggcc tttgttgcat
       61 aggcagctat gatcatacct tctatgacag catggggaat tccttccata atacacatat
      121 ccataaaggc accaggatct ccctcatccc cattacagat tacatatttc atatccgatt
      181 cataggatag gacctgggac cattttatac ctgcaggaaa accaccgcca ccccttcccc
      241 ttaaattgga ttctactatt tcatcgcata cttctttagg actcatttca tttaaaactt
      301 tagctaatgc taaatatcca ccatttccta tgtattcttc tatggattca ggatcgattt
      361 caccacaatc ctttaaaacc aagcgatttt gatttatata aaatggaatg gaatgtttag
      421 aagtaaatac ttcctcccct tgggtaaata ataattcatc tattggatta tccttaagga
      481 aggtctcgtg gattatatct ttacaatgct ccaccttgac cttggtatat aatatatctc
      541 ctggctctat tataactaaa ggtcccgctt cacatagacc tggacatcca ctctttttaa
      601 gctttatatc ttctacttcc tttatcagct tagccttatt tcctaagccc ttttcttcta
      661 ttaatctttt aaattcatca tataccttta aggcaccacc agctacacaa ccggttcctg
      721 cacagaccac tatctgtttt ctttgatttt tgagattttc tttaaactta tcatgatatt
      781 catataagtc ttgactagtt ttaatcttca ttcaaacttc cctccctgat ctttctaact
      841 agctcagtgg ctttattagg agtcatctta gcatagacta caccatctac attgactact
      901 ggtgctagac tacaagcccc tagacaagaa accatttcca ccgtaaacaa aagatcctct
      961 gtagtgcttt tcccttcagg aatgcctaat tcttttctta aggcatctaa tataggttgt
     1021 gattttcgaa tatgacaggc cgtaccgtcg
//
LOCUS       DATGSI010000028         1771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_50227, whole genome shotgun sequence.
ACCESSION   DATGSI010000028 DATGSI010000000
VERSION     DATGSI010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50227"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VK031_00380"
     CDS             <1..869
                     /locus_tag="VK031_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HLS52403.1"
                     /translation="FYLYIGASYFLFAVTFFFGAEKYGAVNWVHIAGVSIQPAEVIKL
                     LIVFILASYYSNHKRFKNKKYSSYIIMAIMYSFIGLLFLQKDLGMALIFYIIYLTLQF
                     IYEKNRPLILVNLLLFAISGTFGYFKFNHVRIRVMTWLNPWKYMDNQGYQITQSLFAI
                     AEGGFFGKGIGLGHPYFIPLSYNDFIFSAICEEMGIFTGIGIIMLFMILVYRGFKIGI
                     SQENIFLRIMALGVSIIFGIQTIIIIGGVIKLIPLTGITLPFISYGGSSMLTSFVALG
                     ILQVASEERIEG"
     gene            875..>1771
                     /locus_tag="VK031_00385"
     CDS             875..>1771
                     /locus_tag="VK031_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLS52404.1"
                     /translation="MKSEIRRIILVLTGFCMVFISFVIYLSYFQVFKSHDIKNNSYNK
                     RLWINEENVQRGAFLDRNGQILVYSEKEKETNERFYRYDSLYSHVIGYSLREYGKSGL
                     ELMFNNELLNISEHTALDEFKNLVLPTSVGNNLKLTIDHGLQEKTHSLLKDKKGAIIV
                     MNPKNGEIYSMVSMPDFNVSNLDENWKWIAENEEKPFINRVTQGMYIPGSTFKIVSAV
                     AALENLHLNLDYTCKGVTKIDGKDFLDYRGQVHGRISLKDALAKSCNTYFAEKAISLG
                     QDKLGQVSERFMINQEIEFDLPV"
BASE COUNT          582 a          234 c          351 g          604 t
ORIGIN      
        1 ttttttattt atatataggg gcctcttact tcttgtttgc agtgactttt ttctttgggg
       61 ctgaaaaata tggggctgtt aattgggtcc atatagctgg agtaagtatt cagccagcgg
      121 aagtaattaa attgttaata gtatttattt tggctagtta ttattcaaac cataaaagat
      181 ttaaaaataa aaaatattca tcctacataa ttatggccat tatgtattct tttataggtt
      241 tactattttt gcaaaaagac ctagggatgg ctttaatatt ttatatcata tatttgactc
      301 tacaatttat ttacgaaaaa aacaggccat taatcctagt aaacctttta ttatttgcca
      361 taagtgggac ctttggatat tttaaattta accatgttcg aatcagggtg atgacctggc
      421 ttaatccttg gaagtacatg gataatcagg gctatcaaat aacccaatct ttatttgcta
      481 tagcagaagg tggttttttt ggaaagggaa taggcctagg tcatccctac tttattcctt
      541 tatcctataa tgactttata ttttctgcaa tttgcgagga aatgggaatt tttacaggta
      601 taggtattat tatgttattt atgattttgg tctatagagg ttttaaaata ggcatcagtc
      661 aagaaaatat ttttcttagg ataatggcac tgggggttag tattattttt ggaatccaaa
      721 caattattat tataggtgga gttattaaac taatcccgtt gacaggaatt accttacctt
      781 ttatttccta tggaggcagt tcaatgttaa ccagctttgt tgccttagga atcctgcagg
      841 tggcctctga agaaagaatt gaagggtgaa taagatgaag agtgagattc gaagaatcat
      901 tttggtactt acaggattct gtatggtatt tattagtttt gtaatctatc ttagttattt
      961 tcaggtattt aaatcccatg acataaaaaa caattcttac aataaaaggt tgtggattaa
     1021 tgaggaaaat gtacaaaggg gtgccttcct agacaggaat ggtcaaatcc ttgtatatag
     1081 tgagaaggag aaggaaacta atgagagatt ttatagatac gatagcctat atagccatgt
     1141 gattggctat agcttaagag aatatggaaa atcaggctta gagcttatgt ttaacaatga
     1201 attgttgaat ataagtgaac atactgcact ggatgaattt aaaaatttag ttttacctac
     1261 ttcagtggga aataatttaa aacttactat tgatcatggt ctacaggaaa agacccatag
     1321 tcttttaaag gataaaaaag gggccataat tgttatgaat cctaaaaatg gggagatcta
     1381 ttcaatggtt agtatgccag actttaatgt atcaaatctg gatgaaaact ggaaatggat
     1441 tgctgaaaat gaggaaaaac catttataaa tagggtcacc cagggtatgt atattccagg
     1501 gtctactttc aaaatagttt ctgccgtggc agccttagaa aaccttcatt tgaatttaga
     1561 ttatacctgt aaaggggtta ctaaaataga cggtaaagac tttttagact atagggggca
     1621 ggtccatggc cgaattagcc tgaaagatgc tcttgctaaa tcatgtaata cttattttgc
     1681 agaaaaagcc atatctttag gccaggataa attagggcag gtaagtgaaa ggtttatgat
     1741 aaaccaagaa atagaatttg acttacctgt t
//
LOCUS       DATGSI010000029         3914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_426690, whole genome shotgun sequence.
ACCESSION   DATGSI010000029 DATGSI010000000
VERSION     DATGSI010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426690"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VK031_00390"
     CDS             complement(<1..1346)
                     /locus_tag="VK031_00390"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HLS52405.1"
                     /translation="MKFKLGKGGQVEIILVKKDADQLSKGQDLYAYLKDKDLFKGNLG
                     QVYFDLSLDKNSLLFLGMGAEEDIDLDSLRTAFHKAGKELMKHKVTSAELILPKFEGI
                     SYKDAACAIAEGFLQSEYAFEKYLTKKKTKPSLKEVYLDASDAKEEEVLAGIKEAEIL
                     VEAIFLARDLVNERAINMYPEVLSQAAKDNLEKLGVQVEIFGQKEIEALNMEAFLAVA
                     EGSDKEPQFIVMTYQGDKDSDEKIALVGKGLTYDSGGYSIKPSTSMDTMFSDMAGSAT
                     VIATMKAIAKAGLKKNVVGIVAACENLISGKAYKPGDIIGSMSGKTIEILNTDAEGRL
                     TLADALWYASKEVKADRIIDLATLTGACVVALGNTTTGAISNDDKLMKDVELAALFSG
                     EPVWQLPSHPDYRELIKGDFADLKNTGGRGAGTITAGLFLEEFVDDTPWVHLDIAGT"
     gene            1535..1747
                     /locus_tag="VK031_00395"
     CDS             1535..1747
                     /locus_tag="VK031_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52406.1"
                     /translation="MVLKNTINLNNLTQQELNCIKEIACNHVTMSKKFEQYANQVQDP
                     QLKQMFQKSSTDAETTAMNLINSLGG"
     gene            1750..2028
                     /locus_tag="VK031_00400"
     CDS             1750..2028
                     /locus_tag="VK031_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HLS52407.1"
                     /translation="MREQDIVNDVLEGTKASINSYTHAIMEASNQQLRTTLQTLRDEA
                     EQIQYQIYQIAEQKGYYTPAPTASTNDIAEIKTSLKSSLGSNTQNPMQ"
     gene            2204..2584
                     /gene="acpS"
                     /locus_tag="VK031_00405"
     CDS             2204..2584
                     /gene="acpS"
                     /locus_tag="VK031_00405"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HLS52408.1"
                     /translation="MLSTGVDIVQVNRIEKLLRDKEDKFLKKIFTQNEIQYIKEKQKD
                     PRTIAGIFAAKEAVAKALGTGIGEISWQDMEIIHDSRGKPRLNINKKLANLLRKGKHD
                     SIELSISHEKEYAVAFAITFRNLN"
     gene            2646..3821
                     /gene="alr"
                     /locus_tag="VK031_00410"
     CDS             2646..3821
                     /gene="alr"
                     /locus_tag="VK031_00410"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HLS52409.1"
                     /translation="MESLGEMRGVRLEIDLDNLAHNIREIKKYVGKDTKVMAIVKANA
                     YGHGAVNVSKTFLENGADKLAVSIVSEGIELRKAGLKAPILILNYTSPDQFHNIIKYR
                     LTQNIFDYRDAKLLSEKAVELGKEAKVHIKIDTGMRRIGLPPTEETIKKIIKINNLPN
                     IKIEGIFSHFAKSDEEDKSFTDLQYRRFNWVINQLEEEKVFIPIKHISNSAAIIDMPE
                     YNLDMVRPGIILYGHYPSEVVNKKRINIKPVLTLKSSISNIKRVPKGVGISYNQIYVT
                     ERDSIIATLPIGYADGYSRLLTGKGQVYIKNKRAPIIGKICMDQMMVDITDIKGVNMD
                     DEVILFGYGNDTYPTIEELAKNIGTVNYEIMCMISRRIPRIYIKDNKTRTIVDYILD"
BASE COUNT         1395 a          645 c          696 g         1178 t
ORIGIN      
        1 gtacctgcaa tatctaaatg gacccatgga gtatcatcta caaactcttc taagaatagg
       61 ccggcagtaa tggtgcctgc acctctacca cctgtatttt taagatctgc aaagtcacct
      121 tttataagct ccctataatc aggatgggaa ggtagttgcc agacaggttc acctgaaaat
      181 agggctgcca attctacatc tttcataagt ttgtcatcat tgcttatggc accagtggta
      241 gtatttccta atgctactac acaagctcct gttaatgtgg ctagatcaat tattctatca
      301 gccttaactt ccttgctggc ataccaaagg gcgtcagcaa gagtcagcct accttcagca
      361 tctgtattta atatttcaat agtttttcca gacatggacc ctataatatc tcccggttta
      421 tatgctttgc ctgagattaa attttcacat gcagccacta tgcccaccac atttttctta
      481 agtcctgcct tagcaatagc cttcatggta gcaattactg tggcagagcc agccatatct
      541 gaaaacatgg tatccatgct agtagatggc ttaattgaat agccaccaga atcataggtt
      601 aagcctttgc caacaagggc aattttttca tctgagtctt tgtctccctg gtaggtcata
      661 actataaatt gtggttcttt atctgaacct tctgccactg ccaggaaggc ctccatattt
      721 aaagcttcaa tttctttttg gccaaatatt tcaacctgga cacctaactt ttccagatta
      781 tccttggctg cctgagataa aacctcagga tacatattta tagccctttc atttactaga
      841 tctctagcta agaatatagc ttccactaaa atctcagctt ctttaattcc tgcaagaacc
      901 tcttcctctt tagcatcaga agcatctaga tagacttcct ttaagctagg tttggttttc
      961 ttctttgtta ggtatttttc aaaagcatac tctgactgca agaatccttc tgctatggca
     1021 caggctgcat ccttgtaaga aatgccttca aacttaggaa ggattaattc cgcactagtt
     1081 actttatgct tcattaattc tttgcctgcc ttatggaaag cagttcttaa agaatcaaga
     1141 tctatatctt cctcagcacc catacctaag aaaagcaggc tattcttatc taaggaaaga
     1201 tcaaaatata cttggcctaa attaccttta aataagtcct tatccttaag ataggcatat
     1261 aaatcctgcc ctttacttag ttgatccgca tctttcttta ccaggataat ttctacctgg
     1321 ccaccttttc caagtttaaa cttcacaaaa acacctcttt ttttttatat tctattatta
     1381 gtatatcaaa aaaactaaaa tatttacata aatttggaga attttggtca ttagccctta
     1441 taaaagttaa aattttaatc aagtgtttaa gaataaaact aatgtaataa cacaaaataa
     1501 gcttgttaaa aaaaataatt aggaggttta aattatggtt ttaaaaaata caataaacct
     1561 taataattta actcagcaag agttaaattg tattaaagaa attgcttgta atcacgtaac
     1621 aatgagtaaa aagtttgagc aatatgcaaa tcaggtccaa gatccacaat taaagcaaat
     1681 gtttcagaag tcaagtacag atgcagaaac tacagccatg aacttaataa attcattagg
     1741 agggtaaaaa tgagagaaca ggacatagta aatgatgttt tggaaggaac taaggccagt
     1801 ataaattctt atactcatgc tattatggag gcttccaatc aacaattaag aacaacttta
     1861 caaaccttaa gagatgaggc tgagcaaatc caataccaga tttatcaaat agccgaacaa
     1921 aaaggatact atacaccggc accaacagcc agcactaatg atatagcaga gatcaaaacc
     1981 agtctcaaat ctagtttagg ttcaaataca caaaatccta tgcaatagaa aaacccttag
     2041 acccctggtc taagggtttt ttagtagggg tatgtaaggc aacattgcta taaaaggcta
     2101 aagggtttat aatataactt aagcaatttt acattaaata cagtaagtaa ctaatatttt
     2161 tcctgcttat gtaatattat taagaaagga tggaattaac ttcatgttaa gcactggagt
     2221 tgatatagtt caggtaaata gaatagaaaa attactgaga gataaagaag ataaattttt
     2281 aaaaaagata tttacacaga atgaaatcca atatattaag gaaaaacaaa aagatccaag
     2341 aactattgct ggcatttttg ctgccaagga ggcagtagcc aaggccctgg gcacaggtat
     2401 aggagaaatt agctggcagg atatggagat tattcatgat agtaggggga agcctagatt
     2461 aaatataaat aagaaactgg ccaacctatt aagaaaagga aaacatgatt ctatagaatt
     2521 atctatttcc catgaaaagg aatatgctgt ggcatttgcc ataaccttta gaaatttaaa
     2581 ttaaatttta tcataattat catatattct aacaaaaaaa gtatatctag taaggagagt
     2641 tagatatgga atctttaggc gaaatgagag gggtaagatt agagatagat ttagataatc
     2701 tggcccataa cataagagag ataaagaaat atgtaggtaa agatacaaaa gtcatggcca
     2761 tagtcaaggc caatgcctat ggacatgggg ctgtgaatgt gtctaaaaca tttttagaaa
     2821 atggagcaga taagctggca gtttctattg tgtctgaagg aatagagcta aggaaggcag
     2881 gtttaaaggc tcctatttta attttaaatt atacatcacc agatcaattc cataatatta
     2941 ttaagtatag gttaactcaa aatatattcg attataggga tgcaaaacta ttatctgaaa
     3001 aggcggttga attaggtaag gaggcaaaag tccatattaa gatagacaca ggcatgagaa
     3061 ggataggcct tcctccaact gaggagacta ttaaaaaaat aattaagatt aataatttac
     3121 ctaatataaa aatagagggg atatttagcc attttgcaaa atcggatgag gaggataaat
     3181 cctttactga tttgcaatat aggaggttta attgggtaat taatcaattg gaggaggaaa
     3241 aagtctttat tcccattaaa catatatcca attcggctgc cataatagat atgccagagt
     3301 acaatctgga tatggtaagg cctggaatta tattgtatgg gcattatcca tcagaggtgg
     3361 ttaataaaaa gagaattaat attaagccag tcctaacctt aaaatccagt atatcaaata
     3421 taaaaagggt gccaaaggga gtaggcatca gttataacca aatttatgtt actgaaagag
     3481 attcaattat tgcaacttta cctattggat atgcagacgg ctattccaga ttgttgacag
     3541 gtaaggggca ggtttatatt aagaataaaa gagcgccaat tattgggaaa atatgtatgg
     3601 atcaaatgat ggtggatatt actgatataa aaggcgtaaa tatggatgat gaagtaatct
     3661 tgtttggtta tggaaatgat acttatccta ccatagagga gctggccaag aacattggaa
     3721 cagtaaatta tgaaattatg tgtatgatta gcagaagaat acctaggatc tatataaaag
     3781 acaataaaac caggactata gtagactaca tcttagatta acaagggcca ggtagattta
     3841 atcagttgac atcaattgta attacaatat ataattaata tatgtcattg cctgtaaatt
     3901 agctttatga atgg
//
LOCUS       DATGSI010000030         1186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_663824, whole genome shotgun sequence.
ACCESSION   DATGSI010000030 DATGSI010000000
VERSION     DATGSI010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663824"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..541
                     /locus_tag="VK031_00415"
     CDS             41..541
                     /locus_tag="VK031_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52410.1"
                     /translation="MIPLIEGDVLPELQGVNPDIKGVYFEHGHFREINMSLNQKEETS
                     LYDQKYPLIALFEDIREQVDSNGVKEAELLIAICYNTRLDYKSKERHNNVIKPILTPV
                     YKALMDRINTNPHFIGYSFPHTKINRPYWGVRSVNEDSVQTIFDDILDAIEINIRIKY
                     LENCKF"
     gene            546..>1186
                     /locus_tag="VK031_00420"
     CDS             546..>1186
                     /locus_tag="VK031_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52411.1"
                     /translation="MAELNVITCVQSGAGASNTGVGSCTFDPKEIIGALLVPKGLALS
                     NSDVIDLKETLISLANEDDRSKRIFPIGTFVELEDGSTDEQTDEFGYGVSKVTGEGTY
                     ILSFRFTNGGKCLHSKLRAFNGAEDSYDVIFVDRQQALLGTMVEKDGEPALGGYALEE
                     LYVPKMTFSDGSTSTGYWIRFTLADVAEVNDRFGFVLPAPQDVFNVIRTVRGM"
BASE COUNT          381 a          143 c          275 g          387 t
ORIGIN      
        1 ttgatatata atgaggtgta taattgacat atttaaagaa atgattcctt tgattgaagg
       61 ggatgtacta cctgaattgc aaggagttaa tcctgatatt aagggggtat attttgagca
      121 tggtcatttt agggagatta atatgtcatt aaaccagaag gaagaaacgt ctttatacga
      181 ccaaaagtac cctttgatag ccttatttga ggatattagg gaacaggttg attctaatgg
      241 ggttaaggag gcggaattgc ttattgctat atgttataat acaagattag attataagtc
      301 aaaagaacgc cataataacg tcattaaacc cattttaaca ccagtatata aggcattgat
      361 ggataggatt aatacaaacc ctcattttat tggttattct ttcccccata ctaagattaa
      421 tagaccttat tggggtgtta gaagcgtaaa tgaggattcg gttcagacta tatttgacga
      481 tatattggat gctatagaaa ttaatataag aataaaatat ttagaaaatt gtaaatttta
      541 gaaaaatggc agaattaaat gttataactt gtgtacaatc aggggctggg gcatctaata
      601 caggtgtagg ctcttgtact ttcgacccta aggaaataat tggggcatta ttagttccta
      661 aggggcttgc attaagcaat agtgatgtta ttgaccttaa agaaacatta atatcattag
      721 ctaatgaaga tgatagaagt aaaaggattt tccctatagg tacttttgta gaattagagg
      781 acggttcaac agatgagcaa acagatgagt ttggttatgg cgtttctaag gttactggag
      841 agggtactta tattttatcg tttagattta ctaatggtgg caagtgtttg catagtaaat
      901 taagagcgtt taatggtgca gaagattctt atgatgttat ctttgtagat agacagcagg
      961 cattattagg aacgatggta gaaaaggacg gcgaaccagc tttggggggg tatgctttag
     1021 aggagttgta tgtgcctaaa atgacgttct ctgatgggag tactagtact ggttactgga
     1081 ttagatttac tttagccgat gtggcagagg ttaatgatag gtttggcttt gtattgccag
     1141 cacctcaaga tgtgtttaat gtcatccgta ctgttagagg tatgat
//
LOCUS       DATGSI010000031         3157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_145048, whole genome shotgun sequence.
ACCESSION   DATGSI010000031 DATGSI010000000
VERSION     DATGSI010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145048"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VK031_00425"
     CDS             <1..632
                     /locus_tag="VK031_00425"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HLS52412.1"
                     /translation="LLTEAKKDQEHLHILVDNNTKIPDRTDYILGEMGYDPRPKLIES
                     AKRLEAAGAEFLVMPCNTAHNFYDDIVKNINIPFLNMIEETAKYIKEKYPEDKNIGLL
                     ATDGTIKAQVYNKIFRKYDLEIITPSAENQKYVYQLIYNIKKDVKQDSLEGFYKAMDD
                     IKDREVNLFIAGCTEISVALDLYKLKGNFVDPLRVVAISAIEFAGGRVK"
     gene            763..838
                     /locus_tag="VK031_00430"
     tRNA            763..838
                     /locus_tag="VK031_00430"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:796..798,aa:Ala,seq:ggc)
     gene            complement(983..1321)
                     /locus_tag="VK031_00435"
     CDS             complement(983..1321)
                     /locus_tag="VK031_00435"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HLS52413.1"
                     /translation="MKRHVAIFLVLVLAAATLVGCGGAKYNDGTYQGEAQGAHGPVKV
                     SVEIKDGEIANVEVTEHEENMDHAGPAFDQIPKAIVDKNSTDVDAESGATVTSNAIKE
                     AVDKALEEAK"
     gene            complement(1419..2318)
                     /gene="rocF"
                     /locus_tag="VK031_00440"
     CDS             complement(1419..2318)
                     /gene="rocF"
                     /locus_tag="VK031_00440"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HLS52414.1"
                     /translation="MEVNLIGVPIKYGSNIEGVQYGPDKLREANIVEIFRENKHTIYD
                     LGNIYVPKLSQADIYKDHKKMKFLNPVKMVNENLAHAVYCSLNSQHFPFIIGGDHSLA
                     IGSIAGASKHFKNLAVIWVDAHGDINSVESSPSGNIHGMPLAASMGVGHPSLVNLYYE
                     EAKIQAKNVYIVAARDLDPGEVELIENSDINLYSIDDIRSRGLPIILTEIINKLHNSQ
                     VDGIHFSFDIDSLDLTLVPGTGTPVADGLNVDEAKYIIRRLLSTKLITSMDFVELNPI
                     LDNKEEGTTKLSVELIKEIASHL"
     gene            2499..3113
                     /locus_tag="VK031_00445"
     CDS             2499..3113
                     /locus_tag="VK031_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HLS52415.1"
                     /translation="MSKYYYGVQIGKKPGVYKSWPECQTQVNGYKGAIYKKFKDYKEA
                     LAFAKGENIDSKNNNLVEEKDLKKNEMIAYVDGSFNQESKAYGYGVVLITKDGKEELK
                     GKGQEPDLVEMRNVSGELKAAMLAMDVAKGRGVDTLYLHYDYSGIEQWALGNWKTNKI
                     GTKQYKDYYDSIKDELKVVFIKVAAHTGVKYNEEADRLAKEALL"
BASE COUNT         1076 a          503 c          592 g          986 t
ORIGIN      
        1 ttctgttaac agaagctaag aaggatcagg aacatttaca cattttagta gacaataata
       61 ctaaaattcc tgataggaca gattatattc taggtgagat gggttatgac ccaagaccta
      121 agctgataga atctgcaaag agactggaag cggcaggggc agaattttta gtaatgccct
      181 gtaatacagc gcacaatttt tatgatgata ttgtaaaaaa tattaatata ccctttttaa
      241 atatgataga ggaaactgct aaatatataa aagagaaata tccagaagat aaaaatatag
      301 gtcttttggc cactgacgga actattaagg cccaggtata taataaaatc tttagaaaat
      361 atgatttgga gataataact ccttcagcgg aaaaccagaa atatgtatac cagctaatat
      421 acaatattaa aaaggatgta aagcaagata gcctggaagg cttctataag gccatggatg
      481 atataaagga tagggaagtt aatttattta ttgctggttg tacggaaatt tctgtagctc
      541 ttgacctgta taaactaaaa ggaaactttg ttgacccact tagggttgtg gcaataagtg
      601 ctattgaatt tgctggagga agggtaaaat aataataagc tagattaatc tttaatattt
      661 aggttggtgg atgtaattac caacctaaat attaaaaaat atcaaaaatg tattgaaaaa
      721 aatctggatt tgtattatga tatatagtgt cgtacttaat atggggcttt ggcgaagctg
      781 ggatcgcgcc tgactggcag tcaggagagc aggggttcga atcccctaag ctccaccaga
      841 gaaaaaaagc tagactaaaa tagtctagct tttttatgtt taaataggtg tagcccatct
      901 aaggttatat tctttaaaag gttatatgat gtaaaataaa aaactcatta tttttataat
      961 gagtttttta tttcatattt aattattttg cttcctctaa ggccttgtca acagcttcct
     1021 taatagcatt acttgtaact gttgcacctg attcagcatc aacatctgtt gaatttttat
     1081 ctacaatggc ttttggaatc tggtcaaagg ctggtcctgc atggtccatg ttttcttcat
     1141 gttctgttac ttcaacattt gcaatttcgc catctttgat ttcaacagaa acctttaccg
     1201 ggccatgagc tccttgagct tctccttggt aagtaccatc attgtatttt gctccaccac
     1261 aacctactaa ggtagcagca gccaatacaa gaactaaaaa tatagcaaca tgtcttttca
     1321 taaccttttc cctccatata atagtgctat attatattat ataacttaat tttccattta
     1381 tcaaccgaaa acttaacagt aatcaactga atagagattt aaagatggct agcaatctct
     1441 tttatcagtt ctacagacag cttagtagta ccttcttcct tattatctaa tataggattt
     1501 aattctacaa aatccatgga tgtgattaat ttggtagaaa gtaaccttct aattatatat
     1561 ttagcctcat ctacattaag cccatcagct actggagtgc ctgttcctgg tactagggta
     1621 aggtcaagtg agtctatatc aaagctaaaa tgaattccat ccacctgaga attatgaagc
     1681 ttatttataa tctcagttaa aataataggc aatcccctac ttctaatatc atctatcgaa
     1741 tataagttta tatcagagtt ttcaattaat tcaacctccc cagggtctaa atctctggca
     1801 gcaactatat aaacattttt tgcctgaatt ttggcttctt cataatagag attgactagg
     1861 ctgggatggc ctactcccat agatgcggcc aatggcatgc cgtgaatatt accactgggg
     1921 gaagactcaa cactattaat atcaccatgg gcgtctaccc aaattacagc aagattttta
     1981 aagtgtttag aagcacctgc aatgctgcct atagctaaag aatggtctcc acctatgatg
     2041 aagggaaagt gttggctatt aagggaacaa tatactgcat gggctaaatt ttcatttacc
     2101 atttttacgg gatttaaaaa tttcatcttt ttatggtcct tgtaaatatc cgcttgcgat
     2161 agttttggca cataaatatt tcctaaatcg taaattgtat gcttgttttc tctaaatatt
     2221 tccacaatat ttgcttccct aagcttgtcc ggcccgtatt gtaccccttc tatattgctt
     2281 ccatatttaa tgggtactcc tataagattt acttccatat gtcctcactt cctaatgtat
     2341 aataaatata ttatataact attttataaa cctgtcatta gttgggggta actggactaa
     2401 caacaaagag gctggataaa aaaaatcatt catatataat attaattgat atatatgtaa
     2461 ataatctgaa ttaggataag gtttgaagga gagagctaat gtcaaaatat tattatggag
     2521 tacagatagg aaaaaaaccg ggagtctata aaagctggcc tgagtgtcag actcaggtga
     2581 atggatataa gggcgctata tataaaaaat ttaaagatta caaggaagct ttggcctttg
     2641 ctaaagggga aaacatagat tcaaagaata acaatttagt agaagaaaaa gatctaaaga
     2701 aaaatgagat gatagcctat gtggacggca gttttaacca ggaaagcaag gcctatggct
     2761 atggtgtagt attgataact aaagatggga aggaagaatt aaagggaaaa gggcaagaac
     2821 ctgatctggt ggaaatgaga aatgtatctg gggagcttaa ggcagccatg ttagccatgg
     2881 atgtggccaa aggaagggga gtagatacct tgtacttaca ttatgactat tctggtattg
     2941 aacaatgggc cctagggaat tggaaaacca ataagatagg aactaaacag tataaggatt
     3001 actatgattc tataaaggat gaattaaagg tagtatttat taaagtggca gcccatacgg
     3061 gagttaaata taatgaggag gcagatagac tggcaaaaga agctctcctt taaaaagaga
     3121 gcttttcaaa acctatattg ttctgtttca acagttt
//
LOCUS       DATGSI010000032         1201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_663886, whole genome shotgun sequence.
ACCESSION   DATGSI010000032 DATGSI010000000
VERSION     DATGSI010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663886"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VK031_00450"
     CDS             complement(<1..296)
                     /locus_tag="VK031_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52416.1"
                     /translation="MILAIVYDQTFNSGSMYKNPNRMILYSLVVVMVVTKIFLAAGML
                     LEDGYRLGAGIVKFFSGEPKDRYIPSRRRFVAMMAIGVAALPFSTLLYGMIRGR"
     gene            726..>1201
                     /locus_tag="VK031_00455"
     CDS             726..>1201
                     /locus_tag="VK031_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HLS52417.1"
                     /translation="MEKKPRLFLLDAYALIFRGYYAFIRNPQINSKGQNTSAIMGFVN
                     TLFDLIRREQPDHLAVCFDKDGSAERTELYPEYKAHRDKTPQDILDSVPIIKDILKAM
                     HIPAIELSGVEADDIIGTLSKKAEKEGYQVFMVTSDTDYAQLVSENIFMYRPPNRG"
BASE COUNT          436 a          240 c          207 g          318 t
ORIGIN      
        1 tagcgaccgc gaatcattcc gtaaagaagt gtagagaaag gcaaagcggc aacacctatc
       61 gccatcatag cgacaaacct tcgtctagat ggaatatatc tatcttttgg ttcgcccgag
      121 aaaaacttta ctataccagc acctaatcga tagccatctt ctaataacat tccagcagct
      181 aagaatattt ttgtaaccac cataaccacc actaagctat acaaaatcat cctattggga
      241 tttttgtaca tcgacccgct attaaaagtt tggtcgtaga caatagccag aatcataagc
      301 gcgcttactc cccaataaat ataggcagta cgcacatgct tagacagagt cctaaaggcc
      361 ataaaagcat agaaatctgt aagaatataa aacaggaaaa atgtaaataa tatccaacgc
      421 atgccgcgaa aatacgcact tttaaataat cttcattaaa aagaaaggtt ttaacgagac
      481 ttaaacatga aaacagggat ttcatcacct atttaaagtg catttaataa actaaacata
      541 ttagctatca aaatccatct acacccgaag tttccgtctt agattattaa aagagccaaa
      601 actcttgtac tccacaagct aagtttgtat tcgctgacgg aaacaaaagc cgttcgctcg
      661 ataatttgta attttggggt cctgtacata acagacttaa ttaatttaaa atataagacc
      721 aacggatgga gaagaaacct aggctatttt tactcgatgc atatgcttta atattcagag
      781 ggtattatgc ttttatacga aaccctcaaa taaactctaa aggacaaaac acctctgcga
      841 ttatggggtt cgtcaacacg ctgtttgacc tcattaggag agagcagcca gaccacttgg
      901 ccgtatgttt cgataaagat ggaagtgcag agcgaacaga actatatcct gaatataaag
      961 cacatagaga taaaacccct caagatatat tagatagtgt ccctattatt aaagacatcc
     1021 ttaaagcaat gcatattcca gccatagagc tatcaggagt agaagcagac gatattatag
     1081 gtactttatc taaaaaagca gaaaaagaag ggtatcaggt gtttatggtt acttctgata
     1141 cggattatgc acaattagtt tcggaaaaca tctttatgta ccgccctcct aatagaggaa
     1201 a
//
LOCUS       DATGSI010000033        11180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_145160, whole genome shotgun sequence.
ACCESSION   DATGSI010000033 DATGSI010000000
VERSION     DATGSI010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 11180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11180
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145160"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VK031_00460"
     CDS             <1..1492
                     /locus_tag="VK031_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52418.1"
                     /translation="YVDGNEYSRVLNENMAGDSIVNGSILKIGVQGVGRNFPLHGDIA
                     YFRFNDEYFDFSEGSGDTIVSNFGTVMDIRGDVEWRYQEPIKNVKLYTGLGWKDLIED
                     EGWYLHFPGDNNSYVMARDITDLEVGVGDLDAEVRVRINEDQPSSWSSIIQTRFTTSQ
                     DGWGFTRRNRDNSYQSTITYDGSSLISAYSSDDTTWIDLQVLYENSTGTNTFIVDGDI
                     VQIMQNDHFENINLENGHILSMGRYRPGAGTTYMLKGDISYFRFNDEEFLMNEGQGTT
                     ITGSKGTVMDILGDVEWRRDPSVMKVKIKKTPYVRLSDDTLGQFSEMSMNPMSKLNIS
                     NYYEIKFRRIAGGATLFDNIKVFDQGDYNQFRLSIRPFGELISRVSLYVYDGISSTYL
                     SSEYTTRDWSVVRVEVSDDELHLYVDGEFKESMDLSNPIETTDPYFLSNVFYFFNYNY
                     RNEYPSTIDISYIEIDGERFDFVGGGDTVLGSGGSEITLSGNYTWH"
     gene            1503..1832
                     /locus_tag="VK031_00465"
     CDS             1503..1832
                     /locus_tag="VK031_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52419.1"
                     /translation="MIGLIFIIAALMAYIASINYSKLLWGIPVIVSLFSWSILLGLLT
                     LVVFVTLYDPLRKLKGLDANTHNIKGYIRGSNGLYDIWDYRIVLIVEIFALIVLTSET
                     WLTLIPA"
     gene            1808..2191
                     /locus_tag="VK031_00470"
     CDS             1808..2191
                     /locus_tag="VK031_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52420.1"
                     /translation="MAYTNTGVILNVPTEMYPSGWVYEAIDDFRDYSYRSKVYTLNIP
                     KSDVETPSSASTFDNIIGDESVGVLAKVEDIMDDIMDEGVDIEYWVNISDITHNLLPK
                     DNTDMYRDDPVVYTLKIQFYLKQKV"
     gene            2188..3303
                     /locus_tag="VK031_00475"
     CDS             2188..3303
                     /locus_tag="VK031_00475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HLS52421.1"
                     /translation="MRLLALLILLISACSSDDFTKILEKAPDAGPIVYDNSESYNLAL
                     GSYRDSSVIKYCLVENAPLLTYEETVALVEDAVTMWGNIINKPFVQVDSINEANIYFT
                     FEFLDGTGGLLGFAEYPPYFQVKNWNRNITLDKYDIHGDTDLDIVTIIAHEVGHALGI
                     KHSEDPASLMFDQYTGHKPLSLDDNFGARILYNNKKKFYFNQRAYIPIENNEDKISNN
                     FLMKEFFSRCTSFDDDFHFLDENLVYAIQEIRNYYGQPIQIISSYRSYECNIEAGGAL
                     ASKHKEAQALDFRFIGPKARETYHRFMEDITSQNCIFQLLLNIGIGGFGAYNTAFHID
                     TRNTGLHSFFDRSYGLWGKFVEDSYITDPEHPWGIDD"
     gene            3296..3667
                     /locus_tag="VK031_00480"
     CDS             3296..3667
                     /locus_tag="VK031_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52422.1"
                     /translation="MIRVYFIWAILATILAGIFVWRYETKKCIETHITNIADTAIIYI
                     PYHTGLIEDGKPGLKPAAILPSLLRSNKSTSVAIIPNIGYSPYGPTFGIQGVYMKNNW
                     SVSYNYDFILRSHSIGFGWRF"
     gene            3993..4202
                     /locus_tag="VK031_00485"
     CDS             3993..4202
                     /locus_tag="VK031_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52423.1"
                     /translation="MMKFSVLYIAIFKNELLTYGSSVRNLHENLVDHYKISYDYLAKL
                     IRDGDGFIDIDGIKIYRIRRPKKED"
     gene            4638..5438
                     /locus_tag="VK031_00490"
     CDS             4638..5438
                     /locus_tag="VK031_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52424.1"
                     /translation="MTGKSAIVNMSMVMRMEGKLHLFWTYLVVNRICADNGAYMAYDQ
                     LIKEISYNTGRSERTAKVWVRNLLSHRFVYRDNQGNVIIKSKYKLAAYLCGGTATRRC
                     FRVADIYNYQEFKTQLILAAHRYVEDGFYAQYYEVRKWLFSQGNLTAAEEWAFPSDRT
                     KVGCGGRHLADRLGLSYWPVYNVLKGNVVKQWNNYFFSVKDFRKIFNGRGFFMRNPRY
                     KFKRDKKGYLVQCQISSKSTFEGLFNVSRSRIDYEEYELDRAEFIPLS"
     gene            5662..6993
                     /locus_tag="VK031_00495"
     CDS             5662..6993
                     /locus_tag="VK031_00495"
                     /inference="COORDINATES: protein motif:HMM:NF042663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB family ATPase"
                     /protein_id="HLS52425.1"
                     /translation="MVREFELEIMKYILRDPAGVNILEVVPDTFMKDPEMALFLYVVK
                     TYYEEYGTVPHKANTLQYINDVEGISEDVQQVILPLVEVTFEDDFDVDREHLKHKITQ
                     EVQIYKTKTMISKFMDEDKPNFEGMFMEMQNILDIGNQMFEVSSARFALKDFTGFKHI
                     SYENVVRTPFRGMNDMLSAKGLFPPQILVIMAPAKGFKTGTMINFATHCVLQGKKVFY
                     ADFENGVDDIINRIYCRLTQANYLEMRDQITEETLKEVVKFSSVTGGDIYVSDYEMFT
                     GTPGQVRADIEKLIANNWRPDVVIWDYADISSPTLQEHRRKPKHEQIQAVYGEIKNIN
                     KDYGVLSITASQMNRESMKNQVGDATALAGDIGKAANADMIWAMMQTDEEAKKGVGRF
                     HPVLQRTGVRFTGEEFVYLRLDPERQFMEEIDEVDWLNSVDIEIEDDLEDI"
     gene            6990..8207
                     /locus_tag="VK031_00500"
     CDS             6990..8207
                     /locus_tag="VK031_00500"
                     /inference="COORDINATES: protein motif:HMM:NF016359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HLS52426.1"
                     /translation="MKHPIFGTVTTVYWWNRQSTKFYVVNQGGTSSSKTYSIMQVICE
                     LLMEKRRTALVTGMYHHSLVSGAKVDLYEIIARSPILRRAMVNPDAEGEKFLFKNGSR
                     LTFKAYDTADKAKYGKRDILFINEANNMTYAVADQLMKRARERVFIDFNSDARFWVHD
                     NILVYDRHRADYFISNFTHNEYAPEETLEYLRGILQKKTEINNAYWNNEWDVYGLGLT
                     GSVSGTIFQGNFHLIDDFDKVIGLRKKAYFIDFGYTNDPTAVGIAGMKFGNVYAKELL
                     YETGHYGELLALKLKNELQLDPRIPMVCDNDRASVLSLQRAGFKAIIAKKPKVTERLT
                     MMQEVQMFIDKRSTNFQFEQQNYKFKLNKLTMEYTNQPEDEHNHLFDGLGYYLVTVKG
                     MKAKTKPRRAVVR"
     gene            8253..8732
                     /locus_tag="VK031_00505"
     CDS             8253..8732
                     /locus_tag="VK031_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52427.1"
                     /translation="MSELSDTLVRAYSLLLDKPNISHQRALHALIRKICSDNDTRLKI
                     KQVRAMKRSRPIFKNKEVIPNFGGSKDVSDTVVKVEKQTKDDLVTDQILELIEDGEEA
                     LIAEFGSVEAFRLYVEDRYDIDFGRKTAFKSVSDTFKEQVQGVEEEEEDNIDEDVFE"
     gene            8729..9538
                     /locus_tag="VK031_00510"
     CDS             8729..9538
                     /locus_tag="VK031_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52428.1"
                     /translation="MKIELTTAGGKFITLNLPKSPSDVPLRKFIDLDVGYNRLIEWVV
                     GEEEEKSLYENRTYYAYKLAKLLSNYFEVDINDLLDLPAEDLLDDTGQLLPGVLKEHI
                     SNMQGKKDTVPEVTGTLLSILEYLDHILLKYEPKLIGEFEYKGEKWIVPELRVLASNE
                     IQFPKVTVMQAIEAMEVERAAKHIAKDPEQEGSAILTTMLHRIAILCHKEGEEFNPDN
                     FHNKVSLYVHHFKDITMDIALDILFFSQNILENYIKEIVSTTFSTLYQEEE"
     gene            9716..10585
                     /locus_tag="VK031_00515"
     CDS             9716..10585
                     /locus_tag="VK031_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52429.1"
                     /translation="MFDRVLKNHTTIPYKVEVSSLMYYNYDSKKYVPVPDTKAIIRPD
                     TGHIYKLTQDQDTIISPIDILSVLLGKYGAEVIEDSWSASNEVEAVKNVFFVPLGRLY
                     ANTDLECISYLMVGLDYMRLEVKVGAMEDFGEGVNIAWWSPDGAIKINKSLSSYNFRS
                     LMLSMIEDMQGLLEYRYTDYLKLTEQEVLEDEVKSFIEEYHKVHIEDMKNHTDYVTAT
                     VSDMIRRVTGMMNKYGHNAFGLLAGFATYQYHRYNTRRNTYGRITLGLAQKEINKLYE
                     RYAYSDKRGRAKH"
     gene            10740..>11180
                     /locus_tag="VK031_00520"
     CDS             10740..>11180
                     /locus_tag="VK031_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52430.1"
                     /translation="MESREFRGNSIPYREFKSTDIELPEGFKLFYEDSEIKSRLLRYI
                     ERRGFNEEFLESRGVGYCSEGPLVGYIIIPFIIKGRLRYWIGRDGLDRAGMKYINPKG
                     VQQDVMYNETALDKYTEEVHIAEGVFDAWTIGPNAISTLGWQVSD"
BASE COUNT         3806 a         1635 c         2426 g         3313 t
ORIGIN      
        1 gtatgtggat gggaacgaat atagtagagt tttaaacgaa aacatggcgg gagattcgat
       61 tgttaatgga agtattttga aaataggagt tcagggtgtc gggagaaatt tccccctcca
      121 tggcgacatc gcctacttcc gcttcaacga cgaatatttt gacttttccg aggggtcggg
      181 agatacgatc gtgtccaatt tcggtacagt tatggatatt cggggggatg tggaatggcg
      241 ttaccaagaa cctataaaaa atgtaaagtt atacacagga ttaggatgga aagatttgat
      301 tgaagatgaa gggtggtatc ttcattttcc gggagataac aatagctatg tcatggcaag
      361 agacatcacc gatcttgaag taggagtggg agatctcgac gcagaagtca gggtaaggat
      421 aaatgaagac caaccttcaa gttggagttc gattattcag acaagattca ctacttcgca
      481 agacggatgg ggttttacac ggagaaatag agataatagt tatcagtcaa caattacata
      541 cgatgggagt agtttgatat ctgcatacag cagcgatgac actacatgga tagacttgca
      601 agtgctgtat gagaatagca ccggaacaaa tacattcata gttgatggag atatcgttca
      661 gataatgcag aatgatcatt ttgaaaatat aaatcttgaa aatggacaca ttctctccat
      721 ggggagatat cgtccagggg caggcaccac atacatgcta aaaggggata tttcttactt
      781 tcgtttcaat gatgaagaat tcctaatgaa cgaaggtcaa ggaactacca tcaccggatc
      841 gaaaggaact gtaatggata tccttggaga tgtagaatgg cgcagggatc caagtgtaat
      901 gaaggtaaag ataaagaaaa caccttacgt acgtttatcc gatgatactt tagggcaatt
      961 tagtgagatg tctatgaatc ctatgagtaa attgaatatt tcaaactact atgaaataaa
     1021 gtttagacga attgccggag gtgctacatt atttgacaac ataaaagttt ttgatcaagg
     1081 cgattataat cagttcagat tatctattcg accgtttgga gaattaattt ctcgggttag
     1141 tttatatgtt tatgatggga tatcttcaac gtatttatct agcgaataca ccaccaggga
     1201 ttggtctgtt gttagggtgg aagtatctga tgacgagcta cacttatatg tagatgggga
     1261 atttaaagag agtatggatt tatctaaccc tatagaaact acagatcctt attttttaag
     1321 caacgttttc tacttcttta attataatta ccgcaatgaa tacccatcca ctatagatat
     1381 cagttatatt gagatagatg gtgaaagatt tgattttgtg ggtggtgggg atactgtctt
     1441 aggtagtggc gggtctgaaa taactttatc aggtaattac acatggcatt aaaatatgaa
     1501 atatgatagg tttaattttt attattgcag cattaatggc gtacatagcc tcaataaatt
     1561 attctaaatt gttatggggt attcctgtaa tagttagttt attttcctgg tctattttac
     1621 taggtttact aacattggta gtttttgtaa ccttatacga ccccctacgt aaattaaagg
     1681 gcttagatgc aaatactcac aatataaaag gttatattag gggtagtaat ggtttatacg
     1741 atatatggga ttaccgtatt gtattgattg ttgagatatt tgcattaata gtattaacct
     1801 ctgaaacatg gcttacacta ataccggcgt aatattaaac gtacccactg aaatgtaccc
     1861 ctcaggatgg gtatatgaag cgattgacga ctttagggat tactcttaca ggtctaaagt
     1921 ttatacttta aatataccta agagtgatgt agagacacct tcctccgctt ctacatttga
     1981 taacataata ggggacgaat ccgtaggtgt attagccaag gtagaggata ttatggatga
     2041 tattatggat gagggggtag atatagagta ttgggtaaat atcagtgaca tcacccataa
     2101 tttgctccct aaagataata ctgatatgta tagggatgat ccggtagtct acacattaaa
     2161 aatacaattt tacctaaaac agaaagtatg agactattag ccctacttat tttactgatc
     2221 tctgcctgct cctcagatga ctttactaag atattagaaa aagcacccga cgctggaccc
     2281 atagtgtacg ataattcaga atcatataat ttagctttag gttcatatag agatagctct
     2341 gttataaagt attgtttagt tgagaatgct cctcttctaa cttacgaaga gactgtagcc
     2401 ttggtagagg atgctgtaac tatgtggggc aatattatta ataaaccatt tgttcaagtt
     2461 gatagcatca atgaagctaa tatatacttc accttcgaat ttctagatgg tacaggaggt
     2521 ttgttaggtt tcgctgaata tcctccctat ttccaggtaa agaattggaa caggaatatt
     2581 acactggata agtatgatat acatggggat acagacctag atatagttac tatcattgct
     2641 catgaagtag gtcatgcttt aggtataaaa cattctgaag atcctgcatc attaatgttt
     2701 gatcaatata ccggacacaa acccttatcg ttagacgata attttggggc acggatcctt
     2761 tacaataata aaaaaaaatt ctatttcaat cagcgggcat acatacccat agagaacaac
     2821 gaagacaaga tttcaaacaa tttcttgatg aaggaatttt tctctcgttg tactagtttt
     2881 gatgatgact ttcatttttt agacgaaaat ttagtgtacg ctatccagga aattagaaat
     2941 tactacggtc aaccaataca aattatatct tcctacagaa gttatgaatg taatatagaa
     3001 gctggaggag ctttagcatc aaaacataaa gaagcccaag ccttagattt tagatttatc
     3061 ggccctaaag ccagagaaac ttaccacaga tttatggaag atatcacttc acagaactgt
     3121 attttccagt tattattaaa tataggtata ggaggcttcg gtgcttacaa cacggcgttc
     3181 catatcgata ctaggaatac agggctgcat agttttttcg atagatccta tggtttatgg
     3241 ggtaaattcg tagaagattc ttatattaca gatcctgaac atccatgggg tatagatgat
     3301 tagagtttat ttcatatggg ctatacttgc tactatattg gcaggtatct ttgtatggcg
     3361 ttacgaaaca aagaaatgta tcgaaaccca tataacaaat atagctgaca cagctattat
     3421 ctatatccct tatcatacag gtttgattga agatggtaag cctggactaa aaccggccgc
     3481 tatactacct tcactactta ggtctaataa gtctacaagt gttgctatta tacctaatat
     3541 aggttatagc ccttacggcc caacatttgg tatccaagga gtctacatga agaataactg
     3601 gtcagttagt tataactacg attttatatt aagaagtcat tcaataggtt tcggatggag
     3661 attttgaact actttatatg ggtatctatt ttaatagttg taatttattt atttttcaaa
     3721 gtattagata ttctacttca atgggctaca ttacaaacaa tctcacttac agtacaccat
     3781 aaggaaaggg tttactatag gaataaaaag aagtcattct atataatatt cactgataaa
     3841 ggatcgttta aagtagttga tactatagca tttttaaaat ttgacgcttc agacaggtat
     3901 gctagattac atgaagataa aacttataca gtaactacta ccggttatag aagtacttta
     3961 tggtctgagt accctaatat aattaatatc aaatgatgaa attttcagtt ttatatatcg
     4021 ccatctttaa aaacgaattg ttgacttatg gttctagtgt tagaaaccta catgaaaacc
     4081 tagtcgatca ctataaaata agctacgatt acttagctaa acttattaga gatggggatg
     4141 gatttataga tattgatgga attaagatct acagaattag gagacctaaa aaagaggatt
     4201 aaactctact ctaaaaaagg gtttgagtac actataagaa agctacagag ggagcataag
     4261 gttgagatac acttaataaa cacccttaca tctaaatctt actgtacacc tggtaggatt
     4321 taccttggga ctattatgga tgaacactac actaaagtcc tgttccatga gattcaacat
     4381 ttagatcatt ttgataagga caatgaggac gctgaaagtc ttataatacc ctggtaaata
     4441 aaaaaagggg cttttaaagc cccttttatt caaaacgata agtagtgggt ggattagtta
     4501 ggtaatccag gtaagtcaac tgtaaatata caaccctttt ttgtctcaac aaattttatc
     4561 tctataaaat aatttatttg ctgtatcgtt aggttttatg tattatagcc cttatgttag
     4621 ctaccaaact ataatctatg acgggtaagt cagcaattgt aaatatgagt atggtcatga
     4681 gaatggaggg taaattacac ctgttctgga cctatcttgt agttaataga atatgcgccg
     4741 acaatggtgc gtatatggct tacgatcagt taataaaaga aatatcttat aatacaggta
     4801 ggagtgagcg taccgctaaa gtatgggtac gtaatctact tagtcataga ttcgtgtata
     4861 gagataatca aggtaatgtc ataataaaat ccaagtacaa gttagcagct tatttgtgtg
     4921 gcggcaccgc taccagaaga tgtttccggg ttgcagacat atacaattac caggaattta
     4981 aaactcaact tatcttagca gctcacagat atgttgagga cggtttctat gcacagtatt
     5041 acgaagtcag gaagtggtta tttagtcagg gtaatttaac agcggctgaa gaatgggcat
     5101 ttccaagcga tcgtacgaaa gtgggctgcg gaggcaggca tttagcggat aggttaggtt
     5161 tatcttactg gccggtctat aatgtattaa agggtaatgt agttaagcaa tggaacaact
     5221 acttctttag tgtaaaagac ttcaggaaga tatttaatgg gagaggtttc tttatgagaa
     5281 accctaggta caaattcaaa agagataaaa aagggtacct ggtacagtgt cagatatcaa
     5341 gtaaatctac ttttgaaggg ttattcaatg tatctagaag taggatagat tacgaagagt
     5401 atgagttaga tagggcagag tttatacctt tatcctaatt acctcttact tacttagtca
     5461 attacttaat tacttattta ttttctttaa taattatttt gattaagtta cttcactaaa
     5521 attctaaaat aaaaatctcc tccctttgtt tgcctttacc ccgatggggc taaataatat
     5581 gggtattaat attacaatct aagaaattag ataaggggaa ttttatagga aaaattaagt
     5641 acaaaaaaaa taaggtaata aatggtaaga gaatttgagt tagaaataat gaagtatata
     5701 ttaagggatc cagcaggggt aaacatctta gaagtggtcc ctgatacgtt tatgaaggat
     5761 cctgaaatgg ctttgttcct gtatgttgta aaaacctact acgaagaata tgggacagta
     5821 ccacacaaag ctaataccct ccaatacata aatgatgtag aaggtatttc agaggatgta
     5881 caacaggtta tcttaccttt agtggaagtt acctttgaag atgatttcga tgtcgataga
     5941 gagcatttaa agcacaaaat aacccaggaa gttcagatat ataagaccaa aaccatgata
     6001 tctaaattca tggatgagga taaacctaac ttcgagggca tgtttatgga aatgcagaat
     6061 atactagata taggtaacca gatgtttgag gtgtcatcag ctagattcgc tttaaaagat
     6121 ttcacagggt ttaagcatat aagctacgag aatgtagtta gaaccccttt ccgaggcatg
     6181 aatgatatgt tatcagctaa agggttattc ccacctcaaa tactagttat aatggctccg
     6241 gctaaagggt ttaaaaccgg taccatgatt aacttcgcta cacattgcgt actacaaggt
     6301 aagaaggtat tttatgctga ttttgagaat ggtgttgacg atattatcaa taggatatac
     6361 tgtagactta cccaagctaa ctatttagaa atgcgggatc aaataactga ggagacactg
     6421 aaggaagtgg tgaagttttc ttcagttaca ggtggtgata tatatgtgag tgattatgaa
     6481 atgtttacag gaaccccagg acaagttagg gcagatatag agaaactgat agctaataat
     6541 tggcggccgg acgtagttat atgggattat gcagatattt catcaccaac tttacaggaa
     6601 cataggcgta aacctaagca tgaacagata caggcagtgt atggagagat aaaaaatatt
     6661 aataaagatt atggtgtatt gtctataacc gcctcacaga tgaatagaga gtcgatgaag
     6721 aatcaggtgg gtgatgctac agctttagca ggggatatcg gtaaagctgc gaatgctgac
     6781 atgatatggg caatgatgca gacagatgag gaagctaaaa aaggtgtagg tagattccat
     6841 ccagttcttc agcgtacagg agttaggttt actggtgaag agtttgtata tttacggtta
     6901 gaccctgaac ggcagttcat ggaagagatt gacgaggttg attggttaaa ttcagttgat
     6961 atagaaatag aagatgacct agaggatata tgaaacaccc aatatttgga acagttacca
     7021 ccgtatattg gtggaataga caaagcacta aattttatgt agtaaatcaa gggggtacgt
     7081 catctagtaa aacttattca ataatgcagg tcatatgtga gctgctgatg gaaaagagac
     7141 gtacagcact ggttacgggt atgtatcacc atagtttagt atccggtgct aaagtagact
     7201 tatatgagat aatagctaga tcacccattt taaggagagc tatggttaac ccagatgcag
     7261 aaggtgagaa attcctgttt aagaacgggt ctaggttaac ctttaaagct tacgatacag
     7321 cggataaagc taaatacggt aagagggata tactatttat caatgaagct aataacatga
     7381 cgtacgctgt agctgatcag cttatgaaac gagctcggga gagagtgttt attgacttca
     7441 actcagatgc tagattctgg gttcatgata atatactcgt gtacgataga catagagcag
     7501 attactttat atccaacttc acacataacg agtacgcacc tgaagagaca ttagaatacc
     7561 ttagagggat actacagaaa aagacagaaa ttaacaatgc ttactggaat aatgagtggg
     7621 atgtttatgg gttaggtctt accggatcag tttcaggtac tatatttcaa ggcaatttcc
     7681 atttgataga tgacttcgat aaagtcatag gactcaggaa aaaggcgtac tttattgact
     7741 tcggatatac aaatgaccct acagcagtag gtatagctgg tatgaagttt ggtaatgtat
     7801 atgcgaaaga acttctgtac gaaaccgggc attacgggga actattagct ttaaaattaa
     7861 agaacgagtt acagttagat cccaggatac caatggtttg tgataatgat agagcttcag
     7921 tattatctct acagagagcc ggatttaaag ctatcatagc taagaaacct aaagtaacag
     7981 agcggttaac catgatgcag gaggtgcaga tgtttataga caagagaagt acaaatttcc
     8041 agtttgagca gcagaattat aaatttaagc ttaataagct gaccatggag tacactaacc
     8101 agccagaaga tgagcataac catttgtttg atggtctggg gtactacctg gttacagtta
     8161 aaggaatgaa ggctaagaca aaacctagaa gagcggttgt aagatagttg cgtgataaaa
     8221 ataaattgtt tatatttaca aaaaataaaa ctatgtcaga gttatccgat acactagtta
     8281 gggcgtattc actgttgtta gataagccta atataagtca ccagagggct ttacacgccc
     8341 ttatacgtaa gatttgcagt gataatgata caagattaaa aataaaacaa gtaagagcta
     8401 tgaagcgtag tagacctata tttaaaaaca aagaagttat acctaatttt gggggtagta
     8461 aagatgtttc agacactgta gtaaaggtag agaagcaaac taaagatgat ctagttacgg
     8521 atcagatatt agagttgata gaggatggtg aagaggcatt gattgcagag tttggcagtg
     8581 tagaagcttt tagattatac gtagaagata ggtatgatat tgatttcgga agaaaaaccg
     8641 cattcaagtc agtgtcggat acttttaagg agcaggtgca gggtgtggaa gaagaagagg
     8701 aggataatat agatgaggat gtatttgaat gaagatagaa cttactacgg ccggtggtaa
     8761 gtttataaca ttaaacttac caaaatcacc ctcggatgta cctttacgta agtttataga
     8821 cttagatgta ggttataata ggttgataga atgggtagta ggggaggagg aggagaaatc
     8881 cctatacgag aataggactt actatgcata taagctagct aaactactct caaattattt
     8941 tgaagtagat atcaatgact tattagatct accagctgag gacctactgg atgatactgg
     9001 tcagttgctg cctggagtac ttaaagaaca tataagtaac atgcagggta agaaggatac
     9061 ggtcccagag gttacaggta ctttattaag tattttggaa tacttagatc atatactact
     9121 caagtacgaa cctaaactaa taggtgaatt tgaatataaa ggggagaaat ggattgtacc
     9181 agagttaagg gtactagcca gtaatgagat acaattccct aaggtaacag taatgcaggc
     9241 tatagaggct atggaagtgg aaagagcggc taagcatata gctaaagacc cggaacagga
     9301 aggttcagct atactaacaa caatgctaca tagaatagct atcctatgtc ataaggaagg
     9361 tgaagagttt aatccagata acttccataa caaagtaagt ttatatgtac atcattttaa
     9421 ggatattacg atggatatag ccttagatat actttttttt tctcaaaata tattggagaa
     9481 ttatataaaa gagatcgttt ctactacttt ttcaaccctg taccaagagg aagagtaaag
     9541 gatgctaaat ctgtatcaga ggcagagaaa aagagtcagg atagatttac tatgggtggt
     9601 aaccgtatgt tttatttacg tttaatggag ctaaattttg ccgtagacta taggtctatg
     9661 ctaagctctg attttatgca tgcagcagaa ttactgaatc taaataatat tagcaatgtt
     9721 tgatagggta ctaaagaatc atactaccat accatataaa gtggaagtaa gctcacttat
     9781 gtactataat tacgatagta aaaaatatgt acctgtaccg gacactaaag ctattatacg
     9841 accagacacc gggcacatat ataaactcac acaagatcaa gatacaataa tatctcctat
     9901 agatatttta tccgtgttat taggtaagta cggggcagag gttattgaag attcatggtc
     9961 agcatccaat gaggttgaag cagttaagaa tgtgttcttt gtacctttag gtaggttata
    10021 tgctaatact gatctagagt gtatatctta tttgatggta gggttagatt atatgagatt
    10081 agaagtaaag gtaggagcta tggaggattt cggagagggg gtaaacatag cctggtggag
    10141 cccggatggg gccataaaaa taaataagag cttatcttca tataacttta gaagtttgat
    10201 gcttagtatg atagaggata tgcaaggttt gttagagtac aggtacacag actatctgaa
    10261 acttactgaa caagaggtat tagaggatga ggttaaatcc tttatagagg agtaccataa
    10321 ggtacatata gaggatatga aaaatcatac ggactatgtt acagcaacag tgagtgatat
    10381 gatccgcaga gtaactggga tgatgaataa gtatgggcat aatgcttttg ggttattagc
    10441 cgggtttgct acttatcagt accatagata caacactaga cgcaatactt acggtaggat
    10501 tacgttagga ttagcacaga aagaaataaa taagttgtat gagaggtacg cctacagtga
    10561 taagagaggc cgagctaagc actaatgggt ggtatatatt caattgcccg gtgtgtgatg
    10621 caggtcgaaa taaaaagaaa tgtgcggtac attacgggta caattatgta aagtgttggg
    10681 aatgtggttt acggtgtttt ttagattact atttacgaac attcgaaggg atgccctggt
    10741 tggagagcag ggagtttagg ggaaattcaa taccctatag agaatttaag tctacggata
    10801 tagagttgcc tgaagggttt aagttatttt atgaggatag tgaaataaaa agtagactac
    10861 tacgttatat agaacgtaga ggatttaatg aggagttttt agagagtaga ggggtagggt
    10921 attgttcgga aggtccacta gtagggtaca taataatacc cttcataatt aaaggccgcc
    10981 ttagatattg gataggtaga gatgggttag atagagctgg tatgaagtac attaacccta
    11041 aaggagtaca gcaggatgtt atgtacaatg aaacagctct agataaatat acagaagaag
    11101 tacacatagc agaaggggta ttcgatgcat ggactatagg acccaacgct ataagtactt
    11161 taggctggca ggtatccgat
//
LOCUS       DATGSI010000034         1232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_381644, whole genome shotgun sequence.
ACCESSION   DATGSI010000034 DATGSI010000000
VERSION     DATGSI010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381644"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VK031_00525"
     CDS             complement(<1..278)
                     /locus_tag="VK031_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52431.1"
                     /translation="MSNVIISKLNGYRFVPKELLELDPMYNTYEFDSVYYKHQIKDWE
                     QNVYYAQTVQFSDPLSLQVLARIGGQIEIRLYDCEGKRYKVSPFPILMT"
     gene            complement(281..>1232)
                     /locus_tag="VK031_00530"
     CDS             complement(281..>1232)
                     /locus_tag="VK031_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52432.1"
                     /translation="EAVIKTKFSDIEQDAWSRWGLDWTVEGNNIRFEPLEYFFNDNML
                     FEVNLVKNMTVTPLVDKVFNNLDVGYTDRDNDIIGGREEFNIMTSYLAKDNDVIDNTE
                     SLITPYRADVYGIESVRAKTADEESKDNKGDNDVFLIEVDPRFYSDVQAYKPLKFSNF
                     DLVGITDKKDMYNIALSPKRMLYRHMRRLIGMKISDVLTFQTSERNSELESTLWSGTI
                     IENSDVDLTSTSWSYQGMTHNFSSLFQPFVIEVDCVPPYDLLEILSINNRGYVKFTYK
                     GVTVRGFILDIGVKPATMDSYRLKILSYKNNDLSLIKRYY"
BASE COUNT          411 a          250 c          142 g          429 t
ORIGIN      
        1 gtcataagaa tagggaaagg agaaacttta taacgctttc cttcacaatc atataatcgt
       61 atctctatct gaccacctat tcttgctaaa acttgtaatg ataaagggtc gctaaattga
      121 accgtttgag cataataaac attttgttcc cagtccttaa tctgatgttt ataatacact
      181 gaatcaaatt cataagtatt atacataggg tctagttcca acaattcctt tggaacaaac
      241 ctatacccat ttagtttact aattattaca ttgctcatta ctaataatac cttttaatca
      301 aagataagtc attattttta taacttaaaa ttttaagcct ataactgtcc attgttgctg
      361 gcttaacccc tatatctaat ataaatcccc ttactgttac gcctttatat gtaaacttaa
      421 cataacctct attatttata cttaaaatct ctaacaagtc ataaggaggc acacaatcaa
      481 cctctattac aaatggctgg aataaagaac taaaattatg tgtcatgccc tgataagacc
      541 atgaagtact agttaaatcc acatcactat tttctataat tgtaccactc cataaggtag
      601 attctaattc tgaatttctt tcagatgttt ggaatgttaa tacatcgctt attttcatgc
      661 caattaaacg tctcatatgt ctatacagca tacgcttagg gcttaaagct atattataca
      721 tatccttttt atctgtaatg ccaactaaat caaagttaga gaattttaat ggtttataag
      781 cctgaacatc agaataaaat cttggatcaa cctctattaa gaacacatca ttatcccctt
      841 tattatcttt agattcctca tcagctgttt tagcccttac actctctatg ccatatacat
      901 cggctctata tggtgttatt aacgattcag tattatctat aacatcatta tccttagcta
      961 aataagaggt cattatatta aactcctctc taccacctat aatatcatta tctctatctg
     1021 tatagcccac atcaaggttg ttaaaaacct tatcaaccaa aggtgttaca gtcatatttt
     1081 tgaccaaatt aacctcaaat agcatattat cattaaagaa gtattcaagt ggctcaaaac
     1141 gtatattatt accctctaca gtccagtcta aaccccacct tgaccatgca tcttgctcta
     1201 tatcggaaaa cttggttttt ataacggctt ca
//
LOCUS       DATGSI010000035         1029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_617141, whole genome shotgun sequence.
ACCESSION   DATGSI010000035 DATGSI010000000
VERSION     DATGSI010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617141"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VK031_00535"
     CDS             complement(<1..186)
                     /locus_tag="VK031_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52433.1"
                     /translation="MANIEKPIWSKDKQTLTNVLVNNKSTTYNKVSNWYDGTPMDDSK
                     LDDDFVYVKYEGEYLVRN"
     gene            complement(255..>1029)
                     /locus_tag="VK031_00540"
     CDS             complement(255..>1029)
                     /locus_tag="VK031_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52434.1"
                     /translation="DTDYWNEITDNPDSIFTDFLVAVSAAIENLTVGTIQTGADGERT
                     TIGYKPEDSSDPTSDYSKHSIRGYYPSGRVMVTFSYIPEEEPLTFEGVEFKDTFAIIF
                     YKDESGSPVGFVLSGKSLTSESIIEANKLEVKDGGRVGKWDIGKFEGDSYTKLKTQGA
                     FGGLDSPDYVNEIGAELNPFGLLINRTRYLNDQFPTPTEPNYWIRADISGDAILDIKS
                     RSNQTALKINGKIEMGNEEGFTGTINGAEFVNGICVGQA"
BASE COUNT          271 a          230 c          154 g          374 t
ORIGIN      
        1 attacggact agatattctc cttcatactt tacatataca aagtcatcat ctagcttgct
       61 gtcgtccata ggagtaccgt cataccagtt acttacttta ttatatgtcg tagatttgtt
      121 gtttacaagc acgttagtca acgtttgttt atctttactc caaataggtt tttctatatt
      181 agccattctt cagtctcttt cttatgtttg ttttaaatta ttacaaatat aaatcttttt
      241 atcattttta ttggttaagc ctgtccgaca caaataccat ttacaaactc agccccattt
      301 atagttcctg taaacccctc ttcattaccc atctctatct taccgtttat ttttagagct
      361 gtctgattac ttcttgattt aatatctaat atagcatccc cagatatatc tgcccttatc
      421 cagtagttcg gttcagtagg tgtaggaaac tggtcgttca aataccttgt tctgtttata
      481 agtagtccaa acgggtttaa ctctgcgcct atttcgttta catagtcagg agagtccaat
      541 cctccaaacg ctccttgtgt ttttagtttt gtataagaat ctccttcgaa cttacctata
      601 tcccatttac caacccttcc tccgtctttt acctctagct tgttagcttc tataatactt
      661 tcagaagtaa ggctcttacc actaaggaca aaccccactg gagacccaga ctcgtctttg
      721 taaaatataa tggcgaaagt atctttaaac tccacccctt caaaagttaa aggctcttct
      781 tctggtatat aagaaaaggt aaccataacc cttccacttg ggtaataccc ccttatagag
      841 tgttttgagt agtccgaagt cgggtctgat gaatcttctg gtttatagcc tatagttgtt
      901 ctctcaccat ctgcaccagt ctgaatagtt cctacagtaa ggttttctat agccgcagaa
      961 acagctacta agaaatctgt gaatatggaa tctgggttgt ctgttatctc attccagtag
     1021 tctgtatca
//
LOCUS       DATGSI010000036         1853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_381886, whole genome shotgun sequence.
ACCESSION   DATGSI010000036 DATGSI010000000
VERSION     DATGSI010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381886"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="VK031_00545"
     CDS             <1..1199
                     /locus_tag="VK031_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HLS52435.1"
                     /translation="LSWEAAYRLQHGSQLDLRNIPSIVGGDIAKRHGIVLARSTPAKE
                     FNIYTGETIYSALQKCPKLAIVPPNYHLYIKASRALINLLKEYSPLVQQYSIDEAFLD
                     YSNGQGSYLHVAYEIKNRIEKELGFTVNIGISTNKLLAKMASDLKKPNMVHTLFPDEI
                     PNKMWPLPIDKLFMVGSRTKQKLNSRGIFTIGELAKLDQEYIYTWLKKPGLLIWQYAN
                     GLENSPVRPEPEPRKSVGNSTTTSFDVDSQSEAHLILLAISEMIGMRVRNLELKGSVI
                     SVSLKDKNFFTYSQQKKLSIPTSSTQKIYETARDIFNGMWQGKALRQFSISLSELCGN
                     DFFQLSLMDNYDEREEKLDKTIDHLRNKFGYKSVIRSCFLHSGIEPIIGGVVSEEEYP
                     MMSSLL"
     gene            1212..1475
                     /locus_tag="VK031_00550"
     CDS             1212..1475
                     /locus_tag="VK031_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52436.1"
                     /translation="MKVLMQPIEMLAWFNEEKYPVPLRYRVTSENMERVVIKIDRILF
                     KEEEKLAGNRMILYRCEGIINNNRRIFELKYEISTCQWYLFKM"
     gene            1532..1768
                     /locus_tag="VK031_00555"
     CDS             1532..1768
                     /locus_tag="VK031_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52437.1"
                     /translation="MKNNNAISEMINQTRLIEKNNLNNMEYTTNITSLASSEDLAQIR
                     DEKISRKIKELHKQIEDINRLTSDLLNDLTRKYN"
BASE COUNT          657 a          307 c          328 g          561 t
ORIGIN      
        1 acctatcctg ggaggctgcc tatagactac aacatggtag ccaattggat ctaagaaata
       61 tcccctctat tgtaggtggg gatatagcca aaaggcatgg catagtattg gccagatcca
      121 cccctgctaa agagtttaat atttatactg gggagactat ttattccgct ttacaaaaat
      181 gtcccaaact ggccatagtt cctcctaatt atcatttata cataaaggca agcagggccc
      241 taataaattt acttaaagag tactcccctc tggtccaaca atattcaata gatgaggcct
      301 ttttagatta ttctaatggt caaggaagtt atctacatgt agcctatgaa atcaagaatc
      361 gaatagaaaa ggagttaggc tttacagtaa atataggcat cagtaccaac aagcttttgg
      421 ccaaaatggc ctctgattta aaaaagccca atatggttca caccctattt cctgatgaaa
      481 tcccaaacaa aatgtggccc ttgcctattg ataagttatt tatggtagga tccagaacta
      541 aacaaaaatt aaatagtaga ggtattttca ccattggtga attagccaaa ctagatcaag
      601 aatatattta tacttggttg aaaaagccag gtcttttaat atggcagtat gccaatggtc
      661 ttgaaaattc tcctgttcgg cctgaaccag agcctagaaa atctgttggg aattctacca
      721 caacctcctt tgatgtagac agccaaagtg aggcccattt aatcttacta gctatttcag
      781 aaatgattgg tatgagagtg cgcaatctag aattgaaggg tagtgtaata tctgtatcct
      841 tgaaagacaa aaattttttc acctattccc agcaaaaaaa attatctata cccacctctt
      901 ctactcaaaa aatttatgaa acggctagag atattttcaa cggtatgtgg cagggtaagg
      961 ccttaagaca gttttctatt tctttatctg agctttgtgg aaatgacttt tttcaactat
     1021 ccttaatgga taactatgat gaaagggaag aaaaattgga caagaccata gaccatcttc
     1081 gaaataaatt cggttataaa tctgtaatca gatcctgctt ccttcattcc ggcatcgagc
     1141 ctattatagg aggggtagtt tcggaagaag aatatcctat gatgtccagt ctactttaga
     1201 ggtgatatta catgaaggtt ttaatgcaac ccatagagat gttagcatgg tttaatgaag
     1261 aaaaatatcc tgttccctta agatacaggg ttacctctga aaacatggaa agagttgtaa
     1321 tcaaaattga taggattctt tttaaagagg aggaaaaact agcaggcaac aggatgattc
     1381 tttatcgatg tgaaggtata ataaataata accgaagaat atttgaatta aaatatgaaa
     1441 tttccacttg tcaatggtat ttattcaaaa tgtaataatt tttattaaac ttctcatact
     1501 attcatagat aggtttgaag ggatgagaag tatgaaaaat aataatgcca tatcagaaat
     1561 gatcaaccaa acccgtttaa ttgaaaaaaa caatcttaat aatatggaat acactacaaa
     1621 tataaccagc ctggccagtt ctgaagattt ggctcagata agggatgaga agatttcaag
     1681 aaagattaaa gaacttcaca agcaaataga agatataaat agattaacct ctgatttgtt
     1741 aaatgattta actaggaaat ataattaaaa atttattatc tcccatattt tttgtaggga
     1801 gctaattttt atgtaataaa gtaattacca tatcccatta ttgtctaacc taa
//
LOCUS       DATGSI010000037         7868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_4161, whole genome shotgun sequence.
ACCESSION   DATGSI010000037 DATGSI010000000
VERSION     DATGSI010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4161"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..1387
                     /locus_tag="VK031_00560"
     CDS             284..1387
                     /locus_tag="VK031_00560"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HLS52438.1"
                     /translation="MGQIHDIRKREKGKHLQFSDRQFIEYLVRKAYPKKVSVRKLAEA
                     IGCSESTIRRELKRGRVIQLSSELIEYESYSAEIAQQDYDYRATAKGPDLKINRDYAF
                     VEYVENKIIKEKYSPDAVIMELENNDFCHPETGFRFQTRICTKTLYNYIAQGIFPNLT
                     NRDLPREGKSPKRKQKRVRRSYRNVDGKSIWERPEEANNRSEVGHWEMDCIEGSKKDN
                     GSCLLTMVDRAARKALIFKLKNQTQECVINVLDKIERKLGRVKFRKTFKTITVDNGSE
                     FLNHKRMERSLRSKTKKRTEIYYCHPYSSWERGTNEQTNGMIRRFIPKGSMISLITED
                     EIVKLEDWLNNYPRRILNGKSANEMNADLKETA"
     misc_binding    1619..1866
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1942..2946
                     /locus_tag="VK031_00565"
     CDS             1942..2946
                     /locus_tag="VK031_00565"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HLS52439.1"
                     /translation="MIELRNVTKQFNTGDKEIKAVKDLSLKINRGEIYGIIGLSGAGK
                     STLIRCINRLEEPTSGEVIIDGVNLTSLDKEELRQERKNIGMIFQHFNLLNQKTVFEN
                     IAFPLRLTNRNKDEIEKRVDELLQYVDLVDKKYDYPSQLSGGQKQRVAIARAIATNPK
                     VLLSDEATSALDPQTTRSILEILKNIRDKFNITIVMITHQMEVVKEICDKVAIMEGGE
                     IIEENTVEALFSNPKTQTAKNFINAMGSDIQEEIINPGEFTGKIFRLSFIGDSANKPF
                     ISRLSREFDIDINILSGNINKLQSTNIGHLIVEFLGDQDEIDRAISLLEENNVYVEVI
                     "
     gene            2946..3587
                     /locus_tag="VK031_00570"
     CDS             2946..3587
                     /locus_tag="VK031_00570"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HLS52440.1"
                     /translation="MAELFVTATLETLYMVLASTIAALLIGLPLGVLVFITEDNNIWP
                     NAWINRILGGIINIFRSIPFIILMTVLFPFTKLVVGKKIGVTATIVPLTVAAAPFIAR
                     IIESSLNEIDKGVIESSLAMGASVKEIIKMVLIPESLPAIISGITLTIINLIGYSAMA
                     GAIGGGGLGDVAIRFGLYRYEVDVMIMALVVIILLVQGTQYIGNRLVGKIDKR"
     gene            3616..4419
                     /locus_tag="VK031_00575"
     CDS             3616..4419
                     /locus_tag="VK031_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HLS52441.1"
                     /translation="MKRTLWIALIILLSVSFLTACSGGKTSTSNKIVIGVSPDPHAKL
                     IELIVDDLEKEDIEVEIKEFTDYVTPNLALDDGDLDANFFQHTPYLEDFIEKEGVELA
                     SIGGVHVEPMALYSDKIDSIDELEDGAEIAIPNDTVNGGRALILLEAHGLIKLEEGAG
                     LEATERDIAENPKNLTFTPLEAAIIPRALDDVDAAIINGNYALEQGLNPVNDGIIIED
                     ADSPYANIVAVRKGEENMERFKKLMEALQSEKVKNFIEEEYDGAIVPAF"
     gene            complement(4536..5201)
                     /locus_tag="VK031_00580"
     CDS             complement(4536..5201)
                     /locus_tag="VK031_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HLS52442.1"
                     /translation="MDTKSREFSLFCLIVDFGKGSKMVKEAKRLGATGGTIFLGKGSV
                     SSHMLDLLGFSEIRKEVLVIVISRELEDNLYQGLNKKFSLEKPHRGIAFSMPLKYCFG
                     MRKMQGHTRSDKQGVNKVEHEAIFTIVDKGLSEQVLDAAKKAGATGGTVIHGRGSGTQ
                     EKAILFNIEIEPEKEIVLILAPVEDARTIVDSIKERLSIEEPGKGIIFALDVNRTLGL
                     YKA"
     gene            complement(5191..6678)
                     /locus_tag="VK031_00585"
     CDS             complement(5191..6678)
                     /locus_tag="VK031_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HLS52443.1"
                     /translation="MNILAKKFKDVLSSVLPITLIVVLLHFTVVPLESSMFFRFILGS
                     IFIILGLGIFLFGAEIGISPIGNLMGGTIAQTNNPYFVAALGFILGFFINVAEPDIQI
                     LADQVSKASGGILSASLILIVVSTGVGIMVAIGLLRIVYEKPLNKLFTIVYIFIFSLG
                     IWATEEFLAISVDASGATTGAMTTPFILALGLGVSQLKGGKTSEEDSFGLVGLASAGP
                     IFAIMILNIVSGLRDIQGEIETFIPHTSIWEPYFQEIPLRMKESIFTLLPLLIIFAIF
                     NYHKFKLERKNKNNIYKGLAYTYTGLTLFLVGVNAGFMEVGRVMGHEIASLPYPWLLP
                     MIGFLLGMVVVLAEPAVYVLTEQVEEVTAGHIKRKVILIALSMGIAFAVALSMLRIMI
                     PGLKLWHFLLPGFAFAAFLSYKVPPIFVGIAYDSGGVASGPMTATFILAFAQGAANAI
                     PTANVLIDGFGVIAMVAMTPLVAIQVLGLLFKIRANKGGANPNGY"
     gene            6914..>7868
                     /locus_tag="VK031_00590"
     CDS             6914..>7868
                     /locus_tag="VK031_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLS52444.1"
                     /translation="MSYLRLIQPKMQKLAETVASALNVEVAIADKNLTRIVGTGLFYD
                     KLDKDCSEDSLFAKVIESGTPIINLTKDQHCTNCSQREACSEHANMTYPIRNDGRIIG
                     VISFASFDIEQTRIIHLKRDEYFNMLQETAGTIEREIANINITNRLVKGVAEVNDIIN
                     CLNKGIIILNSDKEIIHINSKALEYLNINISEQKIINKNILEFIQGIKLTDTGNKDLV
                     DLWKIDNREVRVIYSINKILIGESEYSLLISFDSMEDIINIAKTYENKEKIYFKNIIG
                     NSKAILDSIKRSRIAAATDSTILIQGSSGTGKELFARSIHNE"
BASE COUNT         2875 a         1246 c         1545 g         2202 t
ORIGIN      
        1 ctttttttat ctttttttat cttgttatta gtctacctat aaggaattga aacttggctc
       61 ggagcaatag caacttatta tttggttagt gttattagtc tacctataag gaattgaaac
      121 acctagcacc aagactgtcg tataaaacat catcaagtta ttagtctacc tataaggaat
      181 tgaaggcgag ttgtaaaatg aagtgacgga aaatcaaaaa ttgaaaccca atgaaatatc
      241 ctatacaatg taaatatcga caaatacaaa ggaggataaa tcattgggtc aaatacatga
      301 tatcagaaaa agagagaaag gtaaacatct tcaatttagt gataggcaat ttatcgagta
      361 tttggtgcga aaagcttatc ctaaaaaggt ttcagttagg aaattagcag aggctattgg
      421 atgtagtgaa tctacaatta gacgagaatt aaagaggggt agggtaattc aactgtctag
      481 tgagctaata gagtatgaaa gttatagtgc agaaatagca caacaagact atgattatcg
      541 tgcaacggcc aaaggaccag atttaaaaat aaacagggat tatgccttcg ttgagtatgt
      601 ggaaaacaaa ataataaagg aaaaatattc gcctgatgca gtaataatgg aattagaaaa
      661 caatgatttc tgccatccag aaacagggtt cagatttcag acaaggatat gtaccaaaac
      721 cctatataat tatatagctc aggggatatt tcctaaccta acaaatcggg acctgccaag
      781 agagggtaaa tctccaaaac gcaaacagaa aagggttaga cgctcttata gaaatgtaga
      841 tggcaagagc atatgggaac ggccggagga ggctaataac cgctctgagg taggtcattg
      901 ggagatggat tgcatagaag gaagtaagaa agacaacggt agttgtttgc taacaatggt
      961 agatagggct gcaagaaaag cattgatatt taaattaaaa aatcaaacac aggaatgtgt
     1021 aataaatgtg ctagataaga tagaaaggaa actaggaaga gtaaaattca gaaaaacctt
     1081 taagacgata acagtagaca acggaagtga gtttctaaat cataaaagaa tggaaagatc
     1141 attaagaagt aaaactaaga agcgtacaga gatatattat tgccatcctt atagctcttg
     1201 ggaaagagga actaacgaac aaaccaatgg gatgataaga agatttatac caaaggggag
     1261 catgatatcg ctaattacgg aggatgaaat agttaaacta gaggactggc taaataatta
     1321 tccaaggaga atacttaatg gtaaaagtgc taacgaaatg aatgctgatt taaaagaaac
     1381 tgcataaagt tttgctctat aatattataa aatcctaaaa atattagtaa agggtcaaac
     1441 cacccttgac aaatatttta gaggctttaa atgtctatct aagcaaaaca aacgtataga
     1501 atatttcttg aaaaacttcg tcacttaaca ttacaatcta gtagaaaata aaaactataa
     1561 aaaaatattg acaaagacaa aaatcattgc tatactaatt aataactttt ataaaacggt
     1621 aagggctttg aagagaagag taggcgtgtg aatactcata cagagagctt cgataggtga
     1681 gaagaagctg aggatagcag gctgaacatg gtctcggagc ttggttgctg aatatattta
     1741 ggcaatcacg tgtgcacacg ttacagtgct agagtattta gggatcttat cctgcgtact
     1801 cgatgaggta ccatatcgtg agataggtat aaataagggt ggtaacacga ggccttcgtc
     1861 cctttgcata agcaaagagg cgaaggcttt ttttattaga taaaagttaa aggaaagatt
     1921 aattctttag ggggaaagaa attgatagaa ctaagaaatg tcacaaaaca attcaataca
     1981 ggagataaag aaataaaggc agttaaggat ctatctttaa agataaatag aggagaaata
     2041 tatgggatta taggtttaag tggggcagga aaatctaccc tcattagatg tattaataga
     2101 ttagaagaac ctacatcggg agaggtaatt atagatggag taaacctaac ttcccttgac
     2161 aaagaggaac taaggcagga aagaaaaaat ataggcatga tctttcaaca ttttaacttg
     2221 ctaaaccaga agactgtttt tgaaaatatt gcctttccgc tgagacttac caatagaaat
     2281 aaagatgaaa ttgaaaaaag agtagatgag cttttgcaat atgtagattt agtagataag
     2341 aaatacgatt atcctagcca gttaagtggt ggccaaaaac aaagggttgc aatagcaagg
     2401 gccatagcta ctaatcctaa ggttttactt agtgatgagg caacttcagc cctagatcca
     2461 cagaccacca gatctatatt ggaaatactt aaaaacatta gagataaatt taatataaca
     2521 attgttatga ttacccacca aatggaggta gtcaaggaaa tatgtgataa ggtggccatt
     2581 atggaaggtg gagaaataat agaagagaat acggtggaag ccttatttag taatccaaag
     2641 actcaaacag caaaaaactt tataaatgct atgggatctg atattcagga ggagataatt
     2701 aatcctggag aatttactgg taagatattt aggctgtctt ttataggaga tagtgccaat
     2761 aagcccttta tatctagatt atctagagaa tttgatatag atataaatat tctatctggg
     2821 aatattaaca aattacagtc cacaaatata ggacatttaa tagttgaatt tttaggagac
     2881 caggatgaaa ttgatagggc aatttcatta ttggaagaaa ataatgtgta tgtggaggtg
     2941 atataatggc tgaactattt gtaactgcga ctttggagac tttatatatg gtcctggctt
     3001 caactattgc tgccttactt ataggcctac ctttaggagt attggtattt ataactgagg
     3061 acaataatat ttggccaaat gcttggataa atagaatttt aggtggaata attaatattt
     3121 ttaggtcaat accttttata attttgatga ctgtgctctt tccttttacc aagctagtag
     3181 taggaaaaaa aataggagtt actgcaacca tagttccttt aactgtggca gcagcaccct
     3241 ttattgccag gatcatagag tcaagtttaa atgaaattga caaaggcgtt atagaatcaa
     3301 gcctggctat gggggcctcc gttaaagaaa taattaaaat ggtgcttata cctgaatccc
     3361 ttccagccat aatatcaggg attaccctaa ccataatcaa tctgataggt tattctgcca
     3421 tggcaggggc cattggaggg ggaggtttag gtgatgtggc cattaggttt gggctgtata
     3481 gatatgaagt cgatgtaatg attatggcct tagttgttat aatactattg gtgcagggaa
     3541 cccagtatat aggcaatagg ctggtaggaa aaatagataa aaggtaataa aaatattaaa
     3601 aatgggggaa taaaaatgaa gaggacatta tggatagctt taattatatt attatcagta
     3661 tctttcttga ctgcttgtag tggaggaaaa acttcaacat ccaacaaaat agtaatagga
     3721 gtatcaccag atccacatgc taaattaata gaattgatag tagatgactt agaaaaagag
     3781 gatatagagg tggaaataaa agagtttaca gactatgtaa ctccaaacct agccttagat
     3841 gatggagatc tagatgccaa ctttttccaa catacacctt atcttgagga ttttattgaa
     3901 aaagagggag tagagcttgc ttccattggt ggagtacatg tggaacctat ggccctttat
     3961 tcagataaga ttgactccat agatgaattg gaagatggag cagaaattgc tattcccaat
     4021 gacacagtaa atggtggtag agcattaatt ttactggagg cacatgggct aattaagtta
     4081 gaagaagggg caggactgga ggctactgaa agagatatag cagagaatcc aaaaaatcta
     4141 accttcacgc ccttagaggc agcaatcata ccaagagcac tggacgatgt ggatgcagct
     4201 attataaatg gaaattatgc cctagagcag ggactaaatc ctgttaatga cggcataata
     4261 atagaggatg cagactcacc atatgcaaat atagttgcag taagaaaagg cgaggaaaac
     4321 atggaaagat tcaaaaaact aatggaagca ttacaatctg aaaaggtgaa aaactttata
     4381 gaagaggaat atgatggagc tatagttcca gcattttaaa ttaatataag caaaagggca
     4441 gacttaattt aggtctgccc ttttgggata aaaatgaaaa tatcttgtgc taaatatcca
     4501 tataaaaaca ccaaactttc aaactacatt tgcttttatg ccttatacag gcctaaggtc
     4561 ctgtttacat ctaaggcaaa tataattcct tttccaggtt cttcaatact aagtctttcc
     4621 ttaattgaat ctactatagt cctggcatct tccaccggag ccaatataag tacaatttct
     4681 ttttcaggct cgatttctat attaaataat atggcctttt cctgggtacc agatcctcta
     4741 ccatgaatga cagtaccacc tgtagctcca gcttttttag cagcatccag gacttgttct
     4801 gacaaacctt tgtcaactat tgtaaatatg gcttcatgtt ccactttatt caccccttgt
     4861 ttatcggatc ttgtatggcc ttgcattttt cgcatcccaa agcaatattt caaaggcatg
     4921 gagaaggcta tgcccctatg gggtttttcc agggaaaact tcttgtttaa cccttggtac
     4981 aaattatctt ccagttccct actgattact ataacaagga cttcttttct gatttctgaa
     5041 aatcctaaca ggtctagcat gtggctactg acagagcctt tacctaaaaa tatggtacct
     5101 ccagtagcac ctaaccgttt tgcctcttta accatcttgc ttcccttgcc aaagtctaca
     5161 atcaggcaaa acaatgaaaa ttcacgactt ttagtatcca ttgggattag cacctccttt
     5221 atttgccctt attttaaata aaagaccaag aacttggata gctaccaaag gagtcatggc
     5281 caccatagcg ataactccaa atccatctat taaaacattg gcagtaggta tagcattggc
     5341 tgcaccttgg gcaaaggcca aaataaaggt ggcagtcata ggacctgagg ctacaccgcc
     5401 tgagtcatag gctataccta cgaaaatagg tggcaccttg taacttaaaa aggcagcaaa
     5461 agcaaatcca ggtaataaaa agtgccacaa tttcaaacca ggaatcatga ttcttaacat
     5521 agataaggca actgcaaaag caattcccat agacagggca attagaataa cctttctttt
     5581 aatatggccg gcagtaactt cctccacctg ctcagttaaa acataaacag caggttcagc
     5641 cagaactacc accataccaa ggagaaagcc aatcataggc aataaccaag ggtatggtaa
     5701 gctggcgatt tcatggccca tcacccggcc aacttccata aaacctgcat taacacctac
     5761 taaaaacaag gttaaaccag tatatgtata tgctaggccc ttgtatatat tgtttttgtt
     5821 ttttctttct agcttaaatt tatgataatt gaagattgca aagattataa gtaagggtag
     5881 gagggtgaaa atagactctt tcatacgaag cggaatctcc tggaaataag gctcccagat
     5941 actggtatgg ggaatgaatg tctctatctc cccttgaata tctcttaaac ctgacactat
     6001 atttagtatc ataatggcga atatggggcc agcagaggca agacctacaa gtccaaagct
     6061 atcttcttca gatgttttac caccctttag ttgagataca cctaaaccta gggctaagat
     6121 gaaaggggta gtcatggcac ctgtagtagc accggaagca tctacagaaa tggctaaaaa
     6181 ctcttctgtt gcccagatac ctaaagaaaa gataaatatg tatactatag taaaaagttt
     6241 atttaagggc ttttcatata caattcttag aagccctatg gcaaccataa ttcctactcc
     6301 cgtagaaacc acaattaaaa taagagatgc agataggata ccaccactgg ccttactaac
     6361 ctgatcggct aaaatttgaa tatcaggttc tgccacattt ataaaaaaac ctaggataaa
     6421 acccaaagct gctacaaaat aagggttatt agtttgagct atggtgccgc ccattagatt
     6481 tcctatggga cttataccga tttcagcacc aaagagaaat atacctaggc ctaagatgat
     6541 aaaaattgaa cctaagatga aacgaaaaaa catggaactt tctaaaggca ccactgtaaa
     6601 atgaagtaga acaacaataa gtgtaatagg gagtacagat gatagaacat ctttaaattt
     6661 cttagctaat atattcatgg caaagcacct tccttttcaa cgctagggaa ttttctacat
     6721 ttaggtgtca ggtaggacaa ataaaataag ttgtaattca tgttataatt catgttataa
     6781 ttaataatat cattaatacc ctaaaagatt aagattaaaa ctagaattaa cttttaattt
     6841 gtatataatg gtttctattg ttaaactata gataaggttt aattattata cataaaccat
     6901 cgaggtgatt tttatgtctt atttaagatt aatacagcct aagatgcaaa agttagcaga
     6961 aactgttgcc tctgccttaa atgtggaggt ggcaatagca gataaaaacc taacaagaat
     7021 tgtaggcacc gggctttttt atgataaatt ggacaaggat tgttcagagg attctctctt
     7081 tgctaaagtt atagaaagtg gtacacctat tataaattta acaaaggatc agcattgtac
     7141 caattgctcc caaagggagg cctgttcgga acatgctaac atgacttacc ctatcagaaa
     7201 cgatggtagg attataggag ttataagctt tgcatccttt gatattgagc aaaccaggat
     7261 aattcattta aaaagagatg aatattttaa catgctccaa gagacagcag gcaccataga
     7321 gcgagaaata gccaatataa atattaccaa tagattggtt aaaggtgttg ccgaagtaaa
     7381 tgacataatt aactgtttaa acaagggtat tattatccta aattctgata aggaaatcat
     7441 tcatatcaat agtaaggctt tagaatattt aaatattaat atatcggagc aaaaaataat
     7501 aaacaaaaat attttagagt ttattcaagg aattaaatta acagacacag gaaataaaga
     7561 cttggtagat ttatggaaga tagataatag agaagttagg gtaatttata gcattaataa
     7621 gattcttata ggagaaagtg aatattccct acttataagc tttgatagta tggaagatat
     7681 aataaacatt gccaagacct atgaaaataa agaaaagatt tattttaaaa acataattgg
     7741 aaatagcaag gcaatattgg attctattaa aagatctagg atcgcagcag ctactgactc
     7801 taccatccta atacaaggct ccagtggaac ggggaaggaa ttattcgcca gatctattca
     7861 taatgaag
//
LOCUS       DATGSI010000038         1734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_617198, whole genome shotgun sequence.
ACCESSION   DATGSI010000038 DATGSI010000000
VERSION     DATGSI010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617198"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VK031_00595"
     CDS             complement(<1..237)
                     /locus_tag="VK031_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HLS52445.1"
                     /translation="MNVKKTYAVAVDGPAGAGKSTVAKKVAEVLNIEYIDTGAMYRAL
                     TYKVLKEGIDPLDSKEVIRLLGSTKIDFNQRSIYL"
     gene            complement(287..646)
                     /gene="aroH"
                     /locus_tag="VK031_00600"
     CDS             complement(287..646)
                     /gene="aroH"
                     /locus_tag="VK031_00600"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HLS52446.1"
                     /translation="MKMIAIRGAITVNENSENAILKATKNLLETMENENNLNKENVVS
                     IIFSVTDDLTKVAPAKAARKLGYTKAGLMCFNEMYVENSLKKCIRVMILYNTDKSQLD
                     IKHVYLEGAKILRPDLL"
     gene            complement(774..1640)
                     /locus_tag="VK031_00605"
     CDS             complement(774..1640)
                     /locus_tag="VK031_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HLS52447.1"
                     /translation="MTERRNLIKLIENSYSQFSKSQKIIADYIVNEYDKAAFMTAATL
                     GQSTGVSESTVVRFAIRLGYEGYREFQRDLQELIKSKLTTIQRLHLAESDYANDEDFI
                     PKIIERDMDNIKRIIDDINMDSFKRAIDLVLEGKNIYIIGLRSSSFLAGYLAFYLNFL
                     FDNIRVIDSGPNDIFEQLLKVNSDDVIIVITYPRYSRRIVDVLDYCKDKGTKIITITD
                     SRLSPAAERAHVSLIAASDMISFVDSLVAPMSLINAFIVALGIEKKENLNNYFEDLEN
                     IWQKFNIYDEIT"
BASE COUNT          556 a          344 c          201 g          633 t
ORIGIN      
        1 tagataaata cttctttggt taaaatcaat cttggtgctc cctaaaagtc ggataacctc
       61 ttttgaatct aaaggatcga ttccttcttt aaggacctta tatgttaggg ccctatacat
      121 ggcaccagta tctatatatt ctatatttaa aacctcagca acctttttag ccactgtgct
      181 tttcccagcc ccagccggtc catctacagc aactgcataa gtctttttca cattcataaa
      241 aaacccccta gcataataat aaccaccaac aggtggttaa atctttttat aataaatcag
      301 gtctaaggat ttttgcacct tccaaataaa catgtttaat atctagctga cttttgtccg
      361 tattgtacag aatcataacc ctaatacatt tttttagact attttcaacg tacatttcat
      421 taaagcacat taaaccagcc ttagtatagc caagttttct agcagcttta gctggagcaa
      481 ccttagtcaa atcatcagtt acagaaaata taatgcttac tacattttcc ttatttaaat
      541 tattttcatt ttccatggtt tccagtaagt ttttggttgc ttttaatata gcattttcac
      601 tattttcatt tactgttata gctcctctaa ttgcaatcat tttcataaca atgctccttt
      661 aataattata tattaaactt atctactata caaggaatac ctatttagaa tatatttaat
      721 attaatatta tcagttatga taagatcttg tttgatatac ttacaaaaaa ttattaggta
      781 atttcatcat aaatattaaa tttttgccaa atattttcta aatcctcaaa ataattattt
      841 aaattttcct ttttctcaat acctaaagca actataaagg catttataag actcataggc
      901 gccactaaag agtctacaaa ggaaatcata tcgcttgcag caattaagct aacatgggcc
      961 ctctcagctg ctggagatag cctactatct gttatagtaa tgatcttggt acccttatct
     1021 ttacaataat ctaatacatc tacaattctt ctagaatacc tgggataagt tattactatg
     1081 ataacatcat ctgaattgac ctttaataat tgttcaaaaa tatcgttagg tccagagtca
     1141 ataacccgta tattatcaaa taaaaaattt aaataaaaag ccaaatatcc ggctaagaat
     1201 gaagagcttc taagcccaat tatatatatg ttctttcctt ctaacaccag atcaattgcc
     1261 cttttaaagg aatccatatt aatgtcatct ataattcttt ttatattatc catatccctt
     1321 tcaataatct taggtataaa gtcttcgtca ttggcataat cactttctgc caaatgaagt
     1381 ctttggattg ttgtcagctt actttttata agttcttgca agtctctctg aaattcccta
     1441 tagccctcat atcctagcct gattgcaaat ctaactacag tagattcact aaccccagta
     1501 gactgaccta gagttgctgc agtcataaag gcagccttgt cgtattcatt aacaatatag
     1561 tctgcaatta ttttttgact cttactgaat tgggaataac tgttctctat aagcttgatt
     1621 aaatttcttc tttctgtcac ccttccccct cctttacatc ctaatcttta aattaatatt
     1681 atcaaatctt tgaattaatg caatatgtta taggcctact ttttccacca cata
//
LOCUS       DATGSI010000039         1680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_239007, whole genome shotgun sequence.
ACCESSION   DATGSI010000039 DATGSI010000000
VERSION     DATGSI010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239007"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VK031_00610"
     CDS             complement(<1..228)
                     /locus_tag="VK031_00610"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HLS52448.1"
                     /translation="MKMTLRWFGSEYDTVKLEEIRQIPGVVGVISTLYGTKPGEVWSR
                     EAIRGLKDEAEAAGLELSGIESVNIHDDIKIG"
     gene            complement(286..768)
                     /locus_tag="VK031_00615"
     CDS             complement(286..768)
                     /locus_tag="VK031_00615"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HLS52449.1"
                     /translation="MTKYQFKYKPSAFELWKLSMYGIMRSMIGVTNLIFSVSMLLLTI
                     RFWDRLYIVLKIILIIAISLFTVIQPVMIYMGARKQVKTVPNDMEIHLDDEGLHIISS
                     KEKSKVKWKDVKGITKVLDMIIIYSTEVHGFIISKRMLGSEKEDFYDYAIGKFKEHRK
                     "
     gene            complement(769..>1680)
                     /locus_tag="VK031_00620"
     CDS             complement(769..>1680)
                     /locus_tag="VK031_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HLS52450.1"
                     /translation="PMMVDQGYDADFSAAVTITSSCEGLLVPPSHNMVIYATTAGGVS
                     VGSLFLAGYIPGAVLAVALMVGAYIISVKRDYPKGSPFSITNLFRQLARSFWALAAVL
                     IVVVGVVTGKFTATESAAIAVIYSLIVSVFIYKGMDWKGVWKALEDVINTLSIVLILI
                     STSAIFGYLLTRLHVPAKAATAIINLTDNPILIALLINLILLVLGMIMDMAPIILIAT
                     PILLPIATSIGIDPVQFGIIVVLNCGIGLLTPPVGSVLFIGSAVSNVRMEKLVKATIP
                     FYICMVVTLLLVTFVPGISMWLPTVFK"
BASE COUNT          549 a          350 c          231 g          550 t
ORIGIN      
        1 ccctatctta atatcatcat gaatattaac actttctatt ccacttaatt ctagacctgc
       61 agcttctgcc tcatccttca agccccttat agcttctcgg ctccatacct cacctggctt
      121 agtgccataa agggttgaaa taactcctac cacaccaggt atttgtctta tttcttctaa
      181 tttaacagta tcatattcac taccaaacca tcttaaggtc attttcatct ttgtaatcaa
      241 ccccgtttat tagaaattct taagtctttt tatagttagt aaatcttatt ttctgtgttc
      301 tttaaacttt cctattgcat agtcataaaa atcttctttt tcagatccaa gcattctctt
      361 ggatataata aacccatgta cttctgttga ataaattatt atcatatcta aaactttagt
      421 tattcccttt acatccttcc actttacctt tgacttttcc ttagaactaa tgatatgaag
      481 tccttcatca tctaaatgaa tttccatatc attaggtaca gtttttacct gttttctggc
      541 tcccatataa atcattacag gttgaataac cgtaaataag gaaattgcaa tgataaggat
      601 tatttttaat actatatata atctgtccca aaaccttatt gttaaaagta gcatagaaac
      661 agaaaatata agattagtta ctcctatcat ggacctcata atcccgtaca ttgacaattt
      721 ccatagttca aaggctgatg gcttatattt aaactggtat tttgtcattt atttaaagac
      781 agttgggagc cacatactga ttcctggtac aaatgtcact agtagaagag taactaccat
      841 acatatataa aatgggattg tggccttaac taatttttcc attcttacat tacttactgc
      901 agaccctata aataatacag aaccaactgg tggcgttaat aatcctattc cacagttgag
      961 tactacgatt ataccaaatt gaactggatc tatcccaatt gaagttgcaa ttggtagtaa
     1021 tattggcgtt gcaatcaata taattggagc catatccata atcattccaa gaactaatag
     1081 gatcaggtta atcaataatg caattagaat tgggttatct gttagattaa ttattgcagt
     1141 ggcagcctta gctggcacat gtaatctagt taaaagataa ccaaaaatag ctgaagtaga
     1201 tattaagatc aatacaatag ataaagtatt tatcacatct tccagcgcct tccatacacc
     1261 tttccaatcc atacccttat agataaagac acttacaatt aaactgtaga taactgctat
     1321 agctgcagat tctgtagcgg taaatttccc tgtaactact ccaactacaa ctattaaaac
     1381 agcagccaag gcccagaagg aacgtgccaa ttgcctaaat aaatttgtaa tactaaatgg
     1441 ggaacccttt ggataatccc tcttaactga gataatataa gcccccacca ttaaagctac
     1501 ggccaatact gcccctggta tatatccagc caagaataaa cttcctacag atactccacc
     1561 tgctgttgtt gcataaataa ccatgttatg acttggtggt accagtagac cttcacaaga
     1621 agatgtaatg gtaacagccg ctgaaaaatc tgcgtcataa ccttgatcca ccatcatagg
//
LOCUS       DATGSI010000040         1345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_285833, whole genome shotgun sequence.
ACCESSION   DATGSI010000040 DATGSI010000000
VERSION     DATGSI010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285833"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VK031_00625"
     CDS             complement(<1..1055)
                     /locus_tag="VK031_00625"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HLS52451.1"
                     /translation="MDFMDLNKYPAEPFKIKSVEPVKMISREEREAAMKEAGYNTFNL
                     RSEDVYIDLLTDSGTNAMSDQQWAAMMVGDEAYAGSKNWIYLESTIKEIFGFKHVVPT
                     HQGRGAENILSSIAITPGQYVAGNMYFTTTRYHQEANGGIFVDIIRDEAHDAGLDIPF
                     KGDIDLDKLENLIKEKGAENIAYVCLAVTVNLAGGQPVSMANMRAVRELTDKYGIKVF
                     YDATRCVENAYFIKEQEEGYADKSIQEIILEMFSYSDGATMSGKKDGIVNIGGFLALN
                     DDELFGKARELVVVYEGMPSYGGMTGRDMQAIATGFREAMQYEYIEHRIKQVRYLGER
                     LKEAGVPIIEPVGGHAV"
BASE COUNT          356 a          310 c          182 g          497 t
ORIGIN      
        1 aatactgcat gtcctcctac tggctctatt attggtactc ctgcttcttt taacctttct
       61 cccaggtatc taacctgttt gattctatgc tctatgtatt catattgcat agcttcccta
      121 aatcctgtag caatagcttg catatctcta ccggtcattc caccatagga aggcattcct
      181 tcatatacaa ctaccaactc tcttgcctta ccaaatagtt catcatcatt tagggctaaa
      241 aatccaccta tatttactat tccatccttt ttacctgaca tggtggctcc atcagaataa
      301 ctaaacattt ctaatattat ttcctggatg ctcttgtcag catagccttc ctcttgctct
      361 tttataaagt aagcattttc tacacatcta gtggcatcgt agaacacttt tatcccatac
      421 ttatcagtta actctctaac agccctcata ttggccatgg atacaggctg tccaccggct
      481 aggttaacag ttacagctaa acatacatag gctatatttt ctgcaccttt ttctttaata
      541 agattttcta acttatctag atctatatca cctttaaatg gaatatctaa tcctgcatca
      601 tgggcctcgt ctctgataat atcaacaaat attcctccgt tggcttcttg gtgatatctt
      661 gtagtggtaa aatacatatt gcccgccaca tattgtccag gtgtaattgc tatactagat
      721 agaatatttt ctgctcctct tccctggtga gttggtacaa catgtttaaa gccaaatatt
      781 tctttaatag ttgattctaa ataaatccag tttttactac ctgcataagc ttcatcacct
      841 accatcatgg ctgcccattg ctggtcactc atggcatttg tacctgagtc ggttagaagg
      901 tcaatataaa catcttctga tcttagattg aatgtgttat acccggcctc cttcatagcc
      961 gcctctcgct cttctctaga aatcattttg accggctcaa cacttttaat cttaaagggt
     1021 tctgccggat atttgtttag atccataaaa tccatcaaat tgcctccttt gttgtaatgt
     1081 ggtttataca tcatgcatga tgcatcatcc atcatttaat attatataag tacaagttct
     1141 ttctgtcaat aggaaaacgt tatttacttt gattttttaa aatccccaat aaagaaaggg
     1201 caagccctgg cttgcccttt cttttattca tctttgttaa cttttatttt ctttctgtaa
     1261 atatttgcac ctaagttggc aaatctttct tctataccat catatcctct ttcaatatga
     1321 taaatattgc taatctcagt tcttc
//
LOCUS       DATGSI010000041         1159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_145580, whole genome shotgun sequence.
ACCESSION   DATGSI010000041 DATGSI010000000
VERSION     DATGSI010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145580"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VK031_00630"
     CDS             complement(<1..589)
                     /locus_tag="VK031_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52452.1"
                     /translation="MEDKSSLGEYLIKIRKVRRTLLTSLKEKLTILRDNSGPFTKYIK
                     RWNPKVLLATFMIVSILAMGFTGYKVHEIKTRALELYLDEDMIAVVRDGDKALATIEE
                     LKADLSREFKANIVLDKEVKFKPGHAKDHEITSEEELKRNIKSKINLLVTGFAIVIDG
                     EEIGVLKTEKEAKDIIEEVKNHYLDHLPEEAKIKEV"
     gene            complement(744..>1159)
                     /locus_tag="VK031_00635"
     CDS             complement(744..>1159)
                     /locus_tag="VK031_00635"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52453.1"
                     /translation="IAYILGVLVTLFKFRRKKITIKIDGKLYSRELILLAAGNGRYYG
                     GGMKILPDAQLNDGYLHLCLVSNAHNLTLLTLFPTIFKGSHVRLKKYVEVFKAKSISL
                     IGQENLDLNFDGEIINNGNEVKFELAHEKLKVIYE"
BASE COUNT          335 a          237 c          142 g          445 t
ORIGIN      
        1 ttacctcttt tatcttggct tcttcaggta aatggtcaag ataatggttc ttaacctctt
       61 ctataatatc ctttgcttct ttttctgttt tcagtactcc aatctcttcc ccatctataa
      121 ctattgcaaa gccagttacc aaaagattaa ttttagattt aatatttctt tttaactcct
      181 cttcactggt tatctcatga tccttagcat ggcccggctt aaattttact tccttgtcca
      241 atactatatt agccttaaat tccctggaca ggtcagcctt caactcttct attgtagcaa
      301 gggccttatc cccatccctt acaacagcta tcatatcttc atctaagtat aattctaagg
      361 ccctggtttt aatctcatga accttgtagc cagtaaagcc catggccaat atacttacta
      421 tcataaaggt tgccaaaagc accttaggat tccatctttt tatgtatttg gtaaatgggc
      481 cactattgtc tctaagtata gtcaactttt ccttcaggga ggttaataaa gttcttctta
      541 ccttccttat ttttattaag tattccccta gggagctttt atcctccatt tgattacctc
      601 ctaaggtaaa ttatagatct atatattaat atatagatct ataattgtaa ctattttgta
      661 atatattcgt attataccat attaaaaatt aatatcaaca atagccagtc cattgttata
      721 tattatattg attaaattct aatttattca taaataacct ttaatttttc atgggccagt
      781 tcaaatttta cctcattgcc attattaatt atctcaccat caaaattaag atctagattt
      841 tcctggccta ttaaactaat actctttgct ttaaatacct ccacatactt tttaagcctt
      901 acatggctac ctttgaaaat tgtaggaaat aaggttagta aggtcaagtt atgtgcatta
      961 cttactaggc aaagatgcag ataaccgtca tttaattggg catctggtaa tattttcata
     1021 ccaccaccat agtatctgcc attgcctgct gccagtaata ttagctccct agaatatagc
     1081 ttaccatcaa tctttatggt tatctttttt cttctgaatt taaatagggt taccagtaca
     1141 cctaatatat aggctattt
//
LOCUS       DATGSI010000042         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_382066, whole genome shotgun sequence.
ACCESSION   DATGSI010000042 DATGSI010000000
VERSION     DATGSI010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382066"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VK031_00640"
     CDS             <1..477
                     /locus_tag="VK031_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HLS52454.1"
                     /translation="VVPEPLIKKTGARIMSLQDPTSKMSKSDEDENSYILLLDDEATI
                     RRKIRRAVTDSVGVIAYNDEQLGIKNLLDIYASFTGKSIEDLVAEYEGAGYGKFKNDL
                     AEVVVEGLRPIREEYDYLTKNKDYLEEVYKTGAKKASYQANKTLRKLYRKIGFVQG"
     gene            complement(503..1501)
                     /locus_tag="VK031_00645"
     CDS             complement(503..1501)
                     /locus_tag="VK031_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLS52455.1"
                     /translation="MSKKFKKLTSIILILAIVVISMTACSKDELTKIKLMEVTHSLFY
                     TPQYVAITQGFFEEEGLEIELINGGGADKVMAALISGEVDIGFMGLEASVYVYNQGRD
                     NYAVNFAQLTQTDGSFLVAREKDDNFTFDKLKGKTIIGGRKGGMPEMTLEYVLKSHGL
                     DLEKDVTVRTDIQFDAMAGAFLAGEGDYVTLFEPVAASLEKEGKAYVVASIGEAAGYI
                     PYTGYSTTKEYMENNEDIIQRFTNALYKAMVWVENSSTEEIAKAVKPQFPDTDDDILI
                     TLVDRYRSQDSWKPDLLITKEGLNHLMDIMELAGELDKRADYEKIVNTKFVENAMK"
     gene            1602..2135
                     /gene="pgsA"
                     /locus_tag="VK031_00650"
     CDS             1602..2135
                     /gene="pgsA"
                     /locus_tag="VK031_00650"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HLS52456.1"
                     /translation="MNIPNLLTTLRIFLIPVYLLVFFSNIRNRLIIAGIIFIIAGISD
                     VLDGYIARKYNQTTKLGTVLDPVADKMMIFAVLISFTWSKLIPPWILIALGLKETVMI
                     LGGIILYFFKGNQVLPSNQYGKVATLFFYISTLSVIFRLPYKATKILFIITVILNLVA
                     FVNYLLIYLSMRKNAAV"
     misc_binding    2179..2393
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2469..>3339
                     /locus_tag="VK031_00655"
     CDS             2469..>3339
                     /locus_tag="VK031_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLS52457.1"
                     /translation="MLENLPKTYNPKDFEDRLYKYWEDNGHFIAPVDENKEPFTIMMP
                     PPNVTGSLHMGHALNNTIQDILIRWKRMEGYAALWLPGTDHASISTEARVVDKLKSEG
                     KSKEELGREKFLEEAWEWTHLYGGTIKRQLRKLGVSCDWSRDKFTLDEDLSFAVEEVF
                     IRLYEKGLIYRGDRIINWCPSCKTAISDAEVEHIDTNSFIWHINYPVKDSDKNLTIAT
                     TRPETMLGDLAVAVNPKDERYQDLVGKTVILPLVNREIPIIADEYVDMEFGTGAVKIT
                     PSHDPNDFEVGERF"
BASE COUNT         1072 a          592 c          657 g         1018 t
ORIGIN      
        1 gtggtaccag aacccctaat taaaaagaca ggggccagga taatgagcct ccaggatcca
       61 accagtaaaa tgagcaagtc agatgaagat gaaaactcct atattctatt gctagatgat
      121 gaggctacca ttaggagaaa aatcagaagg gcggttacag attctgtagg agtaattgcc
      181 tataatgatg agcagctagg gattaaaaat ctattagata tctatgcttc ttttacagga
      241 aaaagtatag aagatttggt ggctgagtat gaaggggcag gctatggtaa gtttaaaaat
      301 gatttggctg aggtagtagt agaagggctc aggccaatta gagaagagta tgattatttg
      361 actaaaaata aggattattt ggaggaagta tataaaactg gtgccaagaa ggcatcttac
      421 caggccaata agaccttgag aaaactctac aggaaaatag gctttgtaca aggataaata
      481 ggtccccatt ttggggacct atttatttca tagcattttc tacaaatttt gtatttacaa
      541 tcttctcata atcagctctt ttatctaatt cacctgccaa ttccataata tccatcagat
      601 ggtttaatcc ctctttggta attagaagat caggcttcca gctatcttga cttctgtatc
      661 tatctactag ggttataaga atatcatcat ctgtatcagg gaattgaggc ttgacagcct
      721 tagcaatttc ttcagtacta ctattttcca cccataccat ggccttatat agggcattgg
      781 taaatctttg gattatatct tcattatttt ccatatactc cttggtagta gaataacctg
      841 tatatggaat gtatccagct gcttctccta tagaggcaac cacataggcc ttgccctcct
      901 tttctaggga ggcggccact ggttcaaaca gggttacata atctccctct ccagctaaga
      961 aagcaccagc catagcatca aactggatat ctgtccttac tgtcacgtcc ttttctaaat
     1021 ctaaaccatg ggactttagt acatattcta aggtcatttc aggcatgccg ccctttctac
     1081 ctccaattat ggtttttccc tttagcttat caaaggtaaa gttatcatcc ttttctctag
     1141 caactaaaaa ggagccgtca gtctgggtta gctgggcaaa gtttactgca taattgtctc
     1201 tgccttgatt atatacataa actgatgcct caaggcccat aaaaccaatg tctacctcac
     1261 cgcttataag ggcagccata accttgtcag ccccaccacc gtttataagt tctatttcta
     1321 aaccttcctc ctcaaagaaa ccttgtgtta ttgccacata ttgcggggta taaaataaag
     1381 agtgggttac ttccattaat tttattttag tcagttcatc cttggaacaa gcagtcatac
     1441 ttataactac aatggccaag attaggatta tggaagttag tttcttaaat ttctttgaca
     1501 tcctatcact ccttgtataa tatattaata taatggttac atatattgat attgtattcc
     1561 tgctcactac attggttact acattttaaa ggagagtatc tatgaatata cctaacttat
     1621 taaccacact acgtatattt cttataccag tatatttact agtatttttt tctaatataa
     1681 gaaacagact aataatagcc ggtattattt ttatcatagc tggaatttcc gatgttttag
     1741 acggatacat agctagaaaa tataatcaaa ccacaaaatt aggtacagta ttggatccag
     1801 tggcagataa gatgatgatc tttgcagtac ttatcagctt tacttggtct aagctcatac
     1861 caccctggat tttaatagcc ctaggactga aggaaacagt aatgatactg ggaggaataa
     1921 ttttatactt ctttaagggg aaccaggttt taccgtctaa ccagtatggc aaggtggcaa
     1981 ctttgttttt ttatatatcc accctgtctg taattttccg tctgccatat aaggctacga
     2041 aaattctttt tataataacg gttattttaa atttagtggc ctttgtaaat tatttattaa
     2101 tctatttatc catgaggaaa aatgctgctg tatagttgac aaatggccat ttaatctgta
     2161 taattaagaa aaaattcata ttagacgatg aggaagagga gtagataaac aactttacag
     2221 agagagggtc ccaggctgag aggcctttaa gcaatgttta tcgaaggtag cttctgagta
     2281 tttaggctga acttaagtag gcctagacgg ttatgccgtt atcatatcaa gtgccataag
     2341 tttcagctta tggaagtaag gtggtaccgc gaaatcaatc tttcgtcctt aatttaggat
     2401 gaaggatttt tttatgctta catttaaaga caattatgca ggacaaactt aagaaaagga
     2461 gtgttattat gttagagaac ttaccaaaaa cttataatcc taaggacttt gaagataggt
     2521 tatataagta ctgggaagat aatggacatt tcatagcccc agtagatgaa aataaagaac
     2581 cctttactat tatgatgcca ccgccaaatg taactggcag tcttcatatg ggccatgcat
     2641 taaacaatac catccaagat attttaatca gatggaaaag gatggaaggt tatgctgcat
     2701 tatggctacc aggcacagac catgccagca tatcaacaga ggctagagta gtagataaac
     2761 taaagtcaga gggcaaatcc aaggaagagc taggccggga aaagttttta gaagaggcct
     2821 gggaatggac ccatctttat ggaggtacca taaagaggca actgcgaaag ctaggtgttt
     2881 cttgtgactg gagccgggat aaatttacat tagatgaaga tttaagtttt gcagtagaag
     2941 aagtatttat tagactatat gaaaaggggc taatttatag gggagatagg ataattaact
     3001 ggtgtccttc ctgtaaaact gctatttcgg atgcagaggt agaacacata gatactaatt
     3061 ccttcatctg gcatataaat tatcctgtga aggatagtga caaaaatctt accatagcca
     3121 ccaccagacc agaaactatg ttgggagatt tggctgtggc tgtaaatcct aaggatgaaa
     3181 ggtaccagga cctggttggc aaaacagtca tactgccctt agtaaacaga gaaataccta
     3241 taattgccga tgaatatgtg gatatggaat ttggaacagg ggcagtaaaa ataacacctt
     3301 cccatgaccc caatgacttt gaagtgggag aaaggtttg
//
LOCUS       DATGSI010000043         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_4511, whole genome shotgun sequence.
ACCESSION   DATGSI010000043 DATGSI010000000
VERSION     DATGSI010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4511"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VK031_00660"
     CDS             complement(<1..634)
                     /locus_tag="VK031_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HLS52458.1"
                     /translation="MIAEIIAIGSELITGSTTNTNAQYLSRKLLELGIEVKYHTAIED
                     DFNLLMDVFNLALNRADLIITTGGLGPTEDDLTKEAISTALGLELKLDLNMKQHIQNI
                     FCNTKRKMTENNLKQALKPAGAEFLENSIGTAPGIFIQQDKKKIILLPGPPREVRLMF
                     ENQVVPQLRSDYNILTRSVNLVGIGESQVETRLMELDLDPINTRFATFARD"
     gene            complement(648..1193)
                     /gene="pgsA"
                     /locus_tag="VK031_00665"
     CDS             complement(648..1193)
                     /gene="pgsA"
                     /locus_tag="VK031_00665"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HLS52459.1"
                     /translation="MNLANKITMFRVVLVPIFILVFNSSLPNAHLYAAIIFIIAALTD
                     TLDGYIARGMDLITTFGKFIDPLADKILVAAALILLVEVGKIPGWVVIIIIAREFTIT
                     GFRILAASEGVTIAASSLGKVKTVTQLIAIILILLDNILFSKIGLPMDMILLYISLFF
                     TVLSGFDYIYKNKHVLNLNDK"
     gene            complement(1248..>3067)
                     /locus_tag="VK031_00670"
     CDS             complement(1248..>3067)
                     /locus_tag="VK031_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HLS52460.1"
                     /translation="ARMETAYSLSLVNKGGGLIGSLIGFFFYKLFGKVGGYLVLLFTT
                     IIGVILLLNIEVKELISQMKQWGKDKSQKKRRTRVNKPKLGKSQITPTDPKDIKILDY
                     AGDNKAETTANKVEEKDINQEIERTNSRAEDIEYDFPPITLLNSPSRKQDSNNKKEIL
                     NNAKMIEETMNNFGIEAMVTQINKGPTITCYELSPSPGIKLSRIVSLSDNIALSLASS
                     DIRIEAPIPGKAAVGIEVPNKVKDNVYLKEIISSQEYKTIDSNTPLALGKDVSGKPIV
                     SSIDKMPHLLIAGATGSGKSVCINTIIMSIIFKSSPGDVKLLLIDPKVVELSIYNEIP
                     HLLIPVVTDAKKAAMALKWAIDEMDNRYKKFAKHNVRDIRAYNEKFASDKGEKMPTIV
                     IIIDELSDLMMVAPQEIEDYICRLAQMARAAGIYLIVATQRPSVDVITGTIKANIPSR
                     ISFAVSSQVDSRTILDMAGAEKLLGKGDMLFYPSFYSKPIRVQGAFISDKEIDSVVKH
                     LRKNNTTYYNEEVVEALENDTSISLSNSDELLPNAIELVVEEGQASISLLQRRLRIGY
                     ARAARIVDEMEAMGVVGGFEGSKPRKVLISKEDLEELNL"
BASE COUNT          898 a          634 c          462 g         1073 t
ORIGIN      
        1 cgtctctagc aaaggttgca aaccttgtat ttattggatc tagatcaagc tccataagcc
       61 ttgtctctac ctgtgattca ccgataccta ctaaatttac agatctggtt aatatattat
      121 agtcacttct aagttgtggt acaacctggt tttcaaacat tagtctaact tcccttggcg
      181 gccctggaag taatattatt ttcttcttat cttgctggat aaatatacct ggagcagttc
      241 caatagagtt ttctaggaat tcagccccag ccggctttaa ggcttgtttt aaattatttt
      301 ctgtcatctt tctcttagta ttgcaaaata tattctgtat atgttgcttc atgtttaaat
      361 ctaattttaa ttctaaacct agagccgtgg atatggcctc cttggttaag tcatcttctg
      421 taggtcctag accacctgta gttattatta gatctgccct gttaagggct aggttaaaca
      481 catccattaa taggttaaag tcatcttcaa tggctgtatg atacttaacc tctatgccca
      541 attctaaaag ttttcttgac aagtactggg cattagtatt tgttgtgctc ccagttatta
      601 gttctgatcc aattgcaata atttctgcaa tcaccctatt acctccgtta tttatcattt
      661 aaatttaaga catgcttgtt cttataaata taatcaaaac ctgatagtac tgtaaaaaat
      721 aaggatatat ataagagaat catatccatt ggtagtccta ttttactaaa caaaatatta
      781 tctaataata ttaatataat ggctattagt tgggttacag tcttaacctt acctagagaa
      841 ctggctgcaa ttgttacccc ttcagaggct gccagtattc taaagcctgt aatagtaaat
      901 tcccttgcta ttattattat aacaacccaa ccagggattt tgccaacttc aaccaagagg
      961 atcaaggctg cagcaactaa tattttatca gctaatggat caataaattt cccaaaggtg
     1021 gtgataagat ccatccccct ggctatatat ccgtccaagg tatcagttag ggcagcaata
     1081 ataaagatta tagctgcata taagtgggca ttaggcaagc tggagttaaa gactaggata
     1141 aaaattggaa ccaaaaccac cctaaacata gttatcttat tggctaaatt catgattaaa
     1201 cctccgtctt ctgtatatta tatgataaat aatttgtaaa tttaaaatta caaattcaat
     1261 tcctctaaat cttccttgct aatcaatact tttctaggtt tgcttccctc aaacccacct
     1321 acaaccccca tagcctccat ttcatcaact attcttgcag ccctagcgta tccaattcta
     1381 agtctccttt gcaagagaga tatagatgcc tgtccttcct caactaccaa ttctattgca
     1441 ttaggtaata actcatcact gttagatagg ctaatggaag tgtcattttc caaagcctct
     1501 accacttcct cattgtaata tgtcgtatta ttcttcctta gatgttttac cacactatct
     1561 atttccttgt cgcttataaa ggctccttgc acccttatag gtttagagta aaaggaagga
     1621 taaaataaca tatctccctt acctaacaat ttctcagccc ctgccatatc tagaattgta
     1681 cgagaatcca cctgtgatga tacggcaaaa gagatcctag aagggatatt agccttaata
     1741 gtacctgtaa ttacatctac agaaggtctt tgggttgcta ctattaaata aatccctgcg
     1801 gccctggcca tctgggccaa tctacaaata taatcttcaa tttcttgagg agccaccatc
     1861 ataagatcag ataactcatc tattataata actattgttg gcatcttttc ccctttatca
     1921 gaagcaaact tctcattata ggcccttata tccctaacat tatgtttagc aaatttttta
     1981 tacctgttat ccatttcatc tatagcccat ttaagagcca tagcagcctt ttttgcatca
     2041 gtaacaactg gtattaaaag atggggtatt tcattgtata tacttaactc aactaccttt
     2101 ggatcgatta aaagcaattt tacatcccca ggtgatgatt taaagataat actcattata
     2161 attgtattta tacatacact ttttcctgag cctgtagcac ctgctattaa aaggtgtggc
     2221 attttatcaa tagatgatac aataggtttg ccactgacat ccttccccag agccaaaggg
     2281 gtgttagaat caatagtttt atattcctgg gaagatataa tttcctttag gtatacatta
     2341 tccttaactt tgttaggaac ctcaattcct acagctgcct taccaggaat aggcgcctca
     2401 attctaatgt ctgaagaggc caggcttaaa gctatattat ccgacaaaga cacaattcta
     2461 ctaagtttta ttcctggtga gggagacagc tcataacaag taatagttgg ccccttgttt
     2521 atctgggtta ccatagcttc tattccgaaa ttgttcatag tctcttctat cattttagca
     2581 ttatttaaaa tttctttttt attattagaa tcctgtttcc tgctagggga atttaaaagt
     2641 gtaataggag gaaaatcata ttcaatatct tccgccctac tgttggtcct ttctatttct
     2701 tgattaatat ccttttcttc cactttattg gctgtagttt cagccttatt atctcctgca
     2761 taatctagta ttttaatatc tttaggatca gtaggtgtta tttggctttt ccctaactta
     2821 ggtttattta cccttgttct tctttttttc tgtgacttat cctttcccca ctgcttcatt
     2881 tgggatatta gttcctttac ttctatattt aataaaagga taaccccaat aattgtggta
     2941 aacaatagga ccaggtatcc tcctactttg ccaaataact tgtaaaagaa aaaacctatc
     3001 agggatccta tcagtccgcc ccctttgttt acaagggata gactataggc cgtctccatc
     3061 ctagcct
//
LOCUS       DATGSI010000044         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_522797, whole genome shotgun sequence.
ACCESSION   DATGSI010000044 DATGSI010000000
VERSION     DATGSI010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522797"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /gene="murA"
                     /locus_tag="VK031_00675"
     CDS             complement(<1..908)
                     /gene="murA"
                     /locus_tag="VK031_00675"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HLS52461.1"
                     /translation="MEIIVVEKSPPLKGNVRINGAKNSALPILAASLLGTEDIILEDV
                     PRLKDVEIMCEVLTSLGAKVEVLGEGTIKINSSNLDNYVTEYELMNKMRASLLVMGPL
                     LARLGKTKSSLPGGCAIGTRPIDLHLKGFKALGATVNTEHGSIEAYVDKLVGNKIYLD
                     FPSVGATENIMMVASMAEGETIIDNAAMEPEIVDLQNFLNKLGADIKGAGTSTIKIKG
                     VKKLGGCTHKIIPDRIEAGTFMVASAITGGDVVVENVIGSHIKPVMAKLQEVGAQVIE
                     NGDNIRVIGNKNLRPIDIKTLPYPGFP"
     gene            complement(924..1712)
                     /locus_tag="VK031_00680"
     CDS             complement(924..1712)
                     /locus_tag="VK031_00680"
                     /inference="COORDINATES: protein motif:HMM:NF020264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HLS52462.1"
                     /translation="MRRVILFAIIILLLIPFFSRADKEYLEYEFLETILQDLEGEFIQ
                     GDVVINGVLIDEFIEKEEIEVLGSNILKKLKLVGRPVDPLIKGNEVTEKTYTKEVIFE
                     SDYNQIIYSGLDKEKNLISIFVSSYIYNEKKEGETYLCINIVKNDDFFEINDIIEDVK
                     SIFKEYDATMEISSCIIGSIKGKVSESYMDKNLNKSLKRIKGRILEEYSDNKALSYTI
                     YTPDIDQFVAVGNRRINLNIAIRYNKDEDSTYLWIGTPIITTGY"
     gene            complement(1848..2258)
                     /locus_tag="VK031_00685"
     CDS             complement(1848..2258)
                     /locus_tag="VK031_00685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HLS52463.1"
                     /translation="MAKFQLEIVTPDRLFFKDEVDMVIVRGCEGDLAVLKGRAPIATP
                     LKIGKVRIFQDDTERVAAVVDGYITVLDNKTTIVTDAAEWPEEIDVERAEAAKRRAEE
                     KLKNRKDAIDVARAEAALKRAINRLEVVNLKKFD"
     gene            complement(2260..>3273)
                     /gene="atpD"
                     /locus_tag="VK031_00690"
     CDS             complement(2260..>3273)
                     /gene="atpD"
                     /locus_tag="VK031_00690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HLS52464.1"
                     /translation="FETGIKVVDLIAPYAKGGKVGLFGGAGVGKTVLIQELINNIATQ
                     HGGISVFSGVGERTREGNDLYYEMIESGVIENTALVFGQMNEPPGARMRVGLTGLTMA
                     EYFRDEEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLAMEMGQLQERITS
                     TNKGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRSIAELGIYPAVDPLDSSSR
                     ILDPAIVGEEHYRVAREVQELLQRYEDLQDIIAILGIDELSEEDRLIVDRARKVQRFL
                     SQPFFVAEQFTGLKGAYVPISETVRGFREILDGKHDDVPESAFFMVGTIDEALEKAKK
                     ADD"
BASE COUNT          907 a          745 c          470 g         1151 t
ORIGIN      
        1 ggaaaaccag gatatggtaa agttttaata tcaataggtc ttagattctt gtttccaata
       61 acccttatat tgtctccatt ttctattacc tgggctccta cttcttgaag cttagccata
      121 acaggcttaa tgtgactgcc tattacattt tctacaacta catctccacc tgtaatagct
      181 gaggccacca taaaggtacc tgcctctatc ctgtcaggta ttatcttatg ggtacaacca
      241 cctaacttct ttaccccttt aatcttaatt gttgatgtac cggccccttt tatgtcagct
      301 cctagcttgt ttaaaaagtt ttgcaaatct acaatttcag gttccatggc cgcattatca
      361 ataattgttt ctccttctgc catagaggca accatcataa tattttctgt agcccctacc
      421 gatgggaagt ctaaatagat tttatttcct accagcttat caacataagc ctctatacta
      481 ccatgttcag tatttacagt ggcacctaag gctttgaaac cctttaaatg taaatctata
      541 ggcctggtac ctatggcaca gccacctggc aaagaacttt tagtctttcc taacctggct
      601 aataaaggac ccattaccaa taaagaggcc ctcatcttgt tcattaattc atattcagtt
      661 acatagttat ctagattgct tgaattaatt tttatggtac cttcccctag gacttcaacc
      721 ttggcaccta aagatgttaa aacttcacac ataatttcta cgtcttttaa tcttggtaca
      781 tcttcaagaa ttatatcttc tgttcctaat aatgaggctg ctagtattgg aagtgctgaa
      841 tttttcgcac cgtttattct tacattacct ttcaaaggtg ggcttttttc cactactata
      901 atttccaatg tatctcctcc tacctaatat ccagttgtta ttataggtgt gcctatccaa
      961 agatatgtac tatcttcatc cttattatat cgtattgcta tgtttaagtt aatcctccta
     1021 ttgccaacag ctacaaattg gtcaatatca ggcgtataaa tagtatatga taaagcttta
     1081 ttgtctgagt attcttccaa gatcctacct ttgattctct tcagtgattt attcaaattt
     1141 ttgtccatgt aagattctga gaccttacct tttatactcc ctattataca gctacttatt
     1201 tccatagttg catcatattc tttgaatatg gatttcacat cttctattat atcatttatt
     1261 tcaaaaaaat catcattttt gactatattt atacaaagat aggtctcccc ctcctttttt
     1321 tcattataaa tgtaggagct tacgaatatg gatattaggt ttttctcctt atctaagcct
     1381 gagtagataa tttggttgta gtcggattca aaaattacct ccttagtata ggttttttca
     1441 gtaacttcat taccctttat taaaggatca acaggccttc ctacaagttt tagtttcttt
     1501 aaaatattac ttcctaaaac ttctatttct tctttttcta taaattcatc aattaagaca
     1561 ccattaatta ccacatcacc ttggatgaat tctccttcca gatcctgtaa aatagtctcc
     1621 aaaaactcat attctaagta ttctttatct gcccttgaga aaaaaggtat tagtaataat
     1681 ataataattg caaataagat aactcttctc atttgtctgc ctcctaagta tattagggaa
     1741 aggccttttt tatcaatgta aagggggaca aaacattgga aataaaaaag tacccttccc
     1801 ttatagtaaa agtattacca aggatgggta ctttttatac ctaaaattta atcaaatttt
     1861 ttcaaattta caacttctaa tctattgatg gctcttttta aagctgcctc tgcccttgcc
     1921 acatctatgg catcttttct gtttttaagc ttttcttcag cccttctttt ggcagcttca
     1981 gccctttcaa catcaatttc ttccggccat tctgcagcat ctgtaactat ggttgtttta
     2041 ttgtctaaga ctgtaatata tccgtctact actgctgcca ctctttcagt atcatcttga
     2101 aaaattctta ccttccctat tttcaaaggg gtagctattg gggcccggcc ctttaaaacg
     2161 gccaaatccc cttcacagcc cctaactatt accatatcta cttcatcttt aaaaaacaat
     2221 ctatcaggcg taactatttc aagctgaaat ttagccatac taatcatccg cctttttagc
     2281 cttttccaga gcttcatcta tggtacctac cataaagaag gctgattctg gtacatcatc
     2341 atgtttccca tctagtattt ccctaaagcc ccttacagtt tcacttatag gtacatatgc
     2401 tccttttagg cctgtaaatt gctcagcaac aaagaagggt tgtgataaga atctttgcac
     2461 cttcctggcc ctatctacaa tcaatctatc ttcttcagat aattcatcaa tacctagtat
     2521 tgcaattata tcctgtaggt cttcatatct ttgtaataat tcttgcacct ctctggctac
     2581 cctgtagtgt tcttccccta ctatagccgg atccagaatt ctagaacttg aatccagtgg
     2641 gtccactgca ggataaatac caagctctgc tattgaacgg gaaagtacag ttgtggcatc
     2701 caagtgagca aaggtggtag ctggtgccgg gtctgttagg tcgtcggctg gtacataaac
     2761 cgcctgcact gatgttatag aacccttgtt agttgaagtg atcctttctt gtaattggcc
     2821 catctccata gccaaggttg gctgataacc aacagcggaa ggcatcctac ctagcaaggc
     2881 tgatacctct gaacctgcct gggtaaatct aaatatattg tctataaata agagaacatc
     2941 ctggccttcc tcatccctaa agtattctgc catagtaagt cctgtaagac ctaccctcat
     3001 cctggctcca ggtggctcat tcatctgccc aaacactaag gctgtatttt caattacacc
     3061 tgactcaatc atttcataat ataggtcatt accttcccta gttctttcac caacaccaga
     3121 aaatactgat ataccaccat gttgggttgc tatgttgtta atcaactctt ggattaatac
     3181 tgtcttacct acacctgcac caccgaataa gcctaccttt ccacctttag catatggtgc
     3241 tatcaggtct acaaccttta ttcctgtttc aaa
//
LOCUS       DATGSI010000045         3632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_239284, whole genome shotgun sequence.
ACCESSION   DATGSI010000045 DATGSI010000000
VERSION     DATGSI010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239284"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /gene="deoC"
                     /locus_tag="VK031_00695"
     CDS             <1..333
                     /gene="deoC"
                     /locus_tag="VK031_00695"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HLS52465.1"
                     /translation="RKDIEAVVKVAKDRAIVKVIIETNLLTEEEKIKACEIVAEAGAD
                     FVKTSTGFSGGGATVEDIKLMKSVVGDRIGIKASGGIRDYRTAQAMIEAGATRLGTSS
                     GVSIVKGM"
     gene            431..877
                     /locus_tag="VK031_00700"
     CDS             431..877
                     /locus_tag="VK031_00700"
                     /inference="COORDINATES: protein motif:HMM:NF021120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcN/YlaJ family sporulation lipoprotein"
                     /protein_id="HLS52466.1"
                     /translation="MRELICIFLVIFLLLQPACVSDPIKNNKNGPKEELIEVTGQDRE
                     ILTRSEDISDLIVDLYGVDDATTIILNHEAIIAIVLARDGVYTEEMKQIIVETAKGQD
                     KGIKNIYISHDKKIFDQIEDIITGLIQGEPYANYLSDINRIMEAIK"
     gene            899..2167
                     /locus_tag="VK031_00705"
     CDS             899..2167
                     /locus_tag="VK031_00705"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HLS52467.1"
                     /translation="MELKSERIKLQEGLHLNLIETEKFKSNLISFYFTRPLNEEEVTK
                     NALLPLVLKSGTKQYPTNLALEKKMEKLYGANFSVAVNKRGERQVIRFTMQWANGEYL
                     GDSEYDYKVIDLLTEIIYNPLLEKDKAFKKDYVAREKKNLTRIINSRINNKRSYAIER
                     CIEEMCSQENFSLYELGRVNDLDHIDEKNLYKHYIDFLATSPIEIFFVGQYDDKLIEY
                     LKQLEQKPREDIIFIPEDKLVEAPKVTRNIKEAFDINQGKLVMGYRSSIKYDDALYNA
                     FLVAGDILGGGANSKLFKHVREEASLAYYISTTIYKYKSILLIDSGIEFEDFERTVEI
                     TKKQIDDLKNGIFTEKDMEVSIQSLKTSTESIKDSIFLIAEFFFSQLLSKDKRTLEQI
                     IEDFRQVKKEEVIEAAKSLSIDTIYFMEKK"
     gene            2186..3478
                     /locus_tag="VK031_00710"
     CDS             2186..3478
                     /locus_tag="VK031_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HLS52468.1"
                     /translation="MNIREKYNEKIHEKVLYSRSNNGLNIFYIPKEGYVKKHAILATN
                     YGSIDNKFIPIGEEDILEVPEGIAHFLEHKLFEEPKANIFDKFSKMGAYVNAYTNYNQ
                     TAYLFYCTDHFYENLELLVRFVQNPYLTEENVEKEKGIIGQEIKMYADDPAWRVHSNL
                     LNSMYYKHPVKIDIGGTLESINTITKDLLYKAYNTFYHPSNMVLFVTGDLDFKKVINT
                     VNQVEKNYDPYPGEIKRIFDEEPEEIRERFIEERMPTSAPLFTIGFKDVDLGYTGEEN
                     IRKDIVTNIILDVLMGSSSHFYNELYKSGLIDADFGSYYTGKSSYGYTLIYGESDEPK
                     TVYGKVQKLVNQPVEKVLKEEDFNRIKNKYLGAFLLRLNSMDFISGNFIELYFDDFLI
                     IDYLDLMDSISYKEVVERFKHHFVEERSALSIIQPSKV"
BASE COUNT         1374 a          447 c          746 g         1065 t
ORIGIN      
        1 agaaaggata ttgaggctgt tgttaaagtg gccaaggata gggctattgt aaaggttatt
       61 atagaaacca atcttttaac tgaagaggaa aaaataaagg cctgtgaaat agtagctgag
      121 gctggtgccg actttgtaaa aacttccaca ggttttagcg gtggaggagc aacagttgaa
      181 gatataaaat taatgaaatc agtagtagga gatcggatag gtataaaagc ttcaggaggc
      241 attagagact atagaaccgc ccaagctatg atagaagccg gggccactag actggggaca
      301 agttcaggag taagcatagt aaaaggaatg taagaattgg ggtgtgaaaa cacccttttt
      361 atttttatta agttttgaaa tttctcataa aattcctaat aaatggaaaa ctattggatg
      421 gagggatttt atgagagagt taatatgtat atttttagtt atttttttac ttttacagcc
      481 tgcctgtgtt agtgatccta taaagaataa taaaaatgga ccaaaagaag aattaataga
      541 ggtaacagga caggacaggg agattctaac tagatctgaa gatatatcag atttgatagt
      601 cgatctgtac ggagttgacg atgctacaac aattattcta aaccatgagg caataattgc
      661 tattgtatta gcaagggatg gggtatatac agaagagatg aaacagatta ttgtagaaac
      721 tgctaagggt caggataaag ggattaaaaa catatatata agtcatgaca aaaaaatatt
      781 tgaccaaatt gaagatatca taactggact aatacaggga gaaccttatg caaattacct
      841 ttctgatata aataggataa tggaggcaat taaataaaat atgcgggagg ggacagacat
      901 ggaattaaag tctgaaagga tcaagttaca agaaggctta catctaaatc taattgaaac
      961 tgaaaaattt aaatctaatt taataagctt ctactttacc aggcccttaa atgaggaaga
     1021 ggttactaaa aatgctcttc ttcctttagt attaaagtca ggcactaaac agtatcctac
     1081 aaatttagcc ttggaaaaaa agatggaaaa gttgtatggg gcaaacttta gtgtggctgt
     1141 taataaaaga ggtgaaaggc aggtaattcg gtttaccatg cagtgggcca atggtgagta
     1201 tctaggagac agtgaatatg attacaaggt tatagacctt ttaacggaga taatatacaa
     1261 cccactattg gaaaaagata aggcctttaa aaaggattat gtagcaagag aaaagaaaaa
     1321 cttaactaga attataaatt caagaattaa taataaaagg tcctatgcca tagaaagatg
     1381 cattgaggaa atgtgtagcc aagaaaactt tagcctgtat gaattaggta gggtaaatga
     1441 tttagaccat atagatgaga aaaacctata taaacattat attgattttt tagctactag
     1501 tcctatagag atattttttg tgggccagta tgatgataag ctgatagaat acttaaagca
     1561 attggagcaa aaacctaggg aggatataat ttttattcct gaagataaat tggtagaggc
     1621 acctaaggtt acaaggaaca ttaaggaagc ttttgacata aaccagggta aactggttat
     1681 gggctataga tcaagtatta aatatgatga tgccttatat aatgcctttc tagtagcagg
     1741 agacatatta ggtggtggcg ccaattctaa gttatttaaa catgtaaggg aggaggccag
     1801 tctagcctat tatatatcta caactattta caaatataag tctatactct taattgatag
     1861 tggtatagaa tttgaggatt ttgaaagaac tgtggaaata acaaaaaaac aaatagatga
     1921 tttgaaaaat ggaatcttta ctgaaaaaga catggaggtt tctatccaat cattgaaaac
     1981 ttcaacggaa tctattaagg acagtatatt tttaatagct gaattctttt ttagtcaatt
     2041 actatcaaag gataaaagaa ctttagagca gattatagag gactttagac aggttaaaaa
     2101 ggaagaggta atagaggctg caaaaagctt atctatagat accatctact ttatggaaaa
     2161 gaaataattg gctaggggtg actttatgaa tattagggaa aaatataatg aaaaaattca
     2221 tgagaaagtt ttatatagca ggtctaataa tggtttaaat attttttata ttcctaagga
     2281 aggttatgta aaaaaacatg ctatattggc tacaaactac ggctccattg acaataaatt
     2341 tatacctata ggtgaagaag acatactgga ggtgccagaa ggtatagctc attttctaga
     2401 acataaactt tttgaagagc caaaggctaa tatatttgat aagttttcaa aaatgggggc
     2461 ctatgtaaat gcatatacca actataatca aacagcctat ctattttatt gtacagatca
     2521 cttctatgaa aatttagaac tactggtaag atttgtacag aacccttact taacagaaga
     2581 gaatgtggaa aaggaaaagg ggattatagg gcaggaaata aaaatgtatg cagatgatcc
     2641 tgcatggagg gtacattcca acttgttaaa cagtatgtat tacaagcatc cagtaaaaat
     2701 agatattggt ggaactttag aaagtataaa taccattacc aaagacctat tatataaggc
     2761 ttataatacc ttttaccatc cctcaaatat ggtacttttt gtaacaggag atttagactt
     2821 taaaaaggta attaatacag tcaaccaggt ggaaaaaaac tatgatccct atccaggtga
     2881 aataaaaaga atatttgatg aagaacctga ggaaattaga gaaagattta ttgaggaaag
     2941 aatgcctaca tctgcacccc tttttactat tgggttcaag gatgtagatt taggatatac
     3001 aggtgaagag aatataagga aggatatagt aacaaatata atactagatg ttttaatggg
     3061 ttccagttcc catttttaca atgaattata taaatctggc ttaattgatg ctgattttgg
     3121 ttcttactat acaggtaaaa gtagttatgg ttatacccta atttatgggg aatcagatga
     3181 acctaaaaca gtttatggca aggttcaaaa gctagtaaat caacctgtag agaaggtttt
     3241 gaaagaagaa gactttaata ggataaaaaa taaatattta ggggccttcc tgttaaggct
     3301 aaactcaatg gactttatat caggtaattt tatagaactg tactttgatg attttctaat
     3361 tattgattat ttagacctaa tggatagcat aagctacaag gaggtggttg aaaggtttaa
     3421 acaccacttt gtggaagaga gaagtgccct atctataatc caaccatcca aagtgtagga
     3481 aatttaccat atttatgata tactaaataa attaggtctt agtgaggaaa ggtggtagat
     3541 atgaatccag tagcatttga aatatttggt atctctatta gatggtacgg tatattaata
     3601 tctatgggag tgctgatagg tgggatcata gc
//
LOCUS       DATGSI010000046         1366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_617589, whole genome shotgun sequence.
ACCESSION   DATGSI010000046 DATGSI010000000
VERSION     DATGSI010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617589"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VK031_00715"
     CDS             <1..222
                     /locus_tag="VK031_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HLS52469.1"
                     /translation="QASAGGGAITATVNGKKELLNIDIDESVVDPEDVEMLEDLVMAA
                     VNEAMRMAEEMVNKEMQKITGGLNIPGLF"
     gene            239..838
                     /gene="recR"
                     /locus_tag="VK031_00720"
     CDS             239..838
                     /gene="recR"
                     /locus_tag="VK031_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HLS52470.1"
                     /translation="MEQYALPIANLIDQLSRLPGIGRKTAQRLAFFILEMEDLDAEKL
                     SKSISEAKEKIRLCSVCCNLTDIDPCHICQSHSRDKSTICVVEGAKDIIAMEKSREYK
                     GEYHVLHGVISPMDNVGPDDIKVKELLARLTNGQVKEIILATNPTVEGEATALYLAKL
                     IKPLGIKVTRIAHGIPVGGDLEYFDEVTLSKAMENRREL"
     gene            982..>1366
                     /locus_tag="VK031_00725"
     CDS             982..>1366
                     /locus_tag="VK031_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HLS52471.1"
                     /translation="MDYRKIEPRNVFTWFYKINQIPRCSGDEKAVSDFLVNFARERNL
                     EVYQDDLYNVIIKKPGTEGYEKSDPVIIQGHMDMVCIKGEGSEHDFTKDPIKMIVEGD
                     ILRADNTTLGGDNGIAIAYGLAILDS"
BASE COUNT          485 a          184 c          326 g          371 t
ORIGIN      
        1 caggccagtg caggcggtgg agctattact gcaactgtaa atgggaagaa ggaattatta
       61 aatatagata tagatgaatc tgtagtagat cctgaagatg tggaaatgtt ggaagacctt
      121 gtaatggcag cagtaaatga agccatgaga atggctgaag aaatggtaaa taaagaaatg
      181 cagaaaatta ctggaggcct aaatatacca ggcttgttct aatttgggag gaattttaat
      241 ggagcagtat gcattaccta tagcaaatct catagaccaa ctgtcaagac tacctggcat
      301 aggtagaaaa actgctcaga gacttgcgtt ttttatatta gaaatggaag atttagatgc
      361 tgaaaagctc tctaaatcta ttagtgaagc caaggaaaaa attagattgt gtagtgtttg
      421 ttgcaaccta actgacatag atccttgtca tatttgtcaa agtcacagta gggataagtc
      481 taccatttgt gtggtggaag gggctaaaga tattatagcc atggaaaagt ctagggaata
      541 caagggagaa taccatgttc tacatggcgt aatctcgcct atggacaatg taggacctga
      601 cgatataaag gttaaggaac ttttagccag acttacaaat ggccaggtta aggagattat
      661 actggcaaca aatcccactg ttgaaggtga agctactgcc ctatatttgg ctaagctaat
      721 taagccctta ggaatcaagg tgaccagaat tgctcatgga attcctgtag gtggagattt
      781 agagtatttt gatgaggtaa ccttgtctaa ggccatggaa aacagaagag aattataaaa
      841 ttagggttcc cttagggaac cctaatttta gtctaaatat gaatggatac tgaactcaat
      901 gtatgaaggg taaaaagtga taatcttgat aaataagtga aatggtatag aatagaataa
      961 accgtaagga ggaagataga aatggattac agaaagattg aaccgagaaa tgtgtttact
     1021 tggttttata agattaacca gattcccagg tgttcaggag atgaaaaggc tgtaagtgac
     1081 tttttagtaa actttgccag ggaaaggaat ctagaagtat atcaggatga tttatataat
     1141 gtgattatta aaaaaccagg tactgaagga tatgaaaaat cagatcctgt aattatccaa
     1201 ggacatatgg atatggtctg tataaagggt gaagggtcag agcatgactt taccaaggat
     1261 cctataaaga tgattgttga gggagatatt ttaagggcag ataatacaac cctaggtggg
     1321 gacaatggaa ttgcaatagc ctacggtcta gccatattag attcaa
//
LOCUS       DATGSI010000047         1570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_145990, whole genome shotgun sequence.
ACCESSION   DATGSI010000047 DATGSI010000000
VERSION     DATGSI010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145990"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VK031_00730"
     CDS             <1..362
                     /locus_tag="VK031_00730"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the conversion of a phosphate monoester
                     to an alcohol and a phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="stationary phase survival protein SurE"
                     /protein_id="HLS52472.1"
                     /translation="KNDKCNFNLAAKYAMDILERLKRGLLDSNLLININTPYFEENQA
                     KGIKVCKIGQVIYDYYFMETDENGDRRLELKGRQEIEFERDTDRYFLSKGYVTVTPLH
                     YDLTNFDLLQEVKGWIL"
     gene            422..937
                     /gene="tpx"
                     /locus_tag="VK031_00735"
     CDS             422..937
                     /gene="tpx"
                     /locus_tag="VK031_00735"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HLS52473.1"
                     /translation="MVNRREGLVTMGGEPVTLLGKETKVGERADNFTVIKNDLSEYSL
                     KDAGDKIKIISVVPSLDTRVCEMQTIEFNKMASQLDEVIILTISVDLPFAQERFCGAQ
                     GIDKVITLSDHRDLDFGMKYGFVMENLRLLARGIVVLDRDNIVQYVEYVKEVREQPNY
                     EQALKEVKKLL"
     gene            989..1468
                     /gene="rlmH"
                     /locus_tag="VK031_00740"
     CDS             989..1468
                     /gene="rlmH"
                     /locus_tag="VK031_00740"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041250629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HLS52474.1"
                     /translation="MKINIIAVGKIKEKYIRDGIKEFEKRLSRYCKLNIIELRDERVP
                     ENLSEKEMELVKIKEGKQILAKIPQNSYIISLEIRGKEISSEILSQKIEKLMISGSSS
                     ITFIIGGSLGLSEEVIKTSNYRLSFSKMTFPHQLIRLILLEQIYRGFKIMKGEPYHK"
BASE COUNT          610 a          191 c          344 g          425 t
ORIGIN      
        1 taaaaaacga taagtgcaat ttcaatttag cagccaaata tgctatggac atactagaaa
       61 ggctaaaaag gggattgtta gattctaatt tactaataaa tataaacaca ccttattttg
      121 aagaaaatca ggccaaaggc atcaaggtgt gcaaaatagg acaggtaatt tatgattatt
      181 actttatgga aacagatgag aatggagaca gaagattaga gctaaagggt agacaagaaa
      241 ttgaatttga gcgggacact gatagatatt tcctatcaaa gggatatgta acagtaactc
      301 ccctacacta cgacctgact aacttcgatt tattgcaaga ggtaaaagga tggatccttt
      361 agtgtagaat ccggtgcaaa gggtaattat atggtacaga aagaagaagg gagtttttaa
      421 aatggtaaat agaagagaag gcctggtgac catgggtggt gagccagtaa cactattagg
      481 taaggaaact aaagtagggg agagggctga taactttact gtaataaaaa acgacctaag
      541 tgaatattct ctaaaggatg caggagataa gataaagatt atatctgtag ttccatcctt
      601 ggacacaagg gtatgtgaaa tgcagacaat agaatttaac aaaatggcat cccaattgga
      661 tgaagttata atcctaacca tatcagtaga tttacccttt gcccaggaaa gattctgtgg
      721 ggcccaaggt attgataaag taataacctt atcagaccat agggatttgg attttggtat
      781 gaaatatggc tttgtaatgg aaaatttaag attattagcc agagggatag tagtcttaga
      841 tagggataat atagtccaat atgtggagta tgtaaaagag gtaagagaac aacccaatta
      901 tgagcaggcc ttaaaggaag ttaaaaagtt gttgtaagat aagggtactc taagagtacc
      961 ctttaaatat gtgaaaaggt agataaaaat gaagataaat ataattgcag taggaaaaat
     1021 aaaagaaaag tacataagag atgggatcaa agaatttgaa aaacgcctat caaggtattg
     1081 taaacttaat ataattgaat taagggatga aagggtgcca gaaaacttgt cagaaaagga
     1141 aatggaactg gtgaaaataa aagaaggtaa gcaaatactg gctaaaattc cccaaaattc
     1201 ttatattatt tccctggaaa taagaggcaa agaaatttcc tcggaaatat tgtcacaaaa
     1261 aatagaaaaa ttaatgattt ctggttccag ctctatcacc tttataatag gaggttctct
     1321 aggcctatca gaagaggtaa taaaaacaag taattatagg ttatcttttt ctaagatgac
     1381 ctttccccac cagctgatac gtttaatatt attggagcaa atatataggg ggtttaagat
     1441 aatgaaaggt gaaccgtatc ataagtagga caagctatgc gttgtaggat aatgtttaag
     1501 gggttactat taattaggat taaatgatat aatgtatata ggtagattaa tgcgtcatgt
     1561 taaatggagg
//
LOCUS       DATGSI010000048         1490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_51022, whole genome shotgun sequence.
ACCESSION   DATGSI010000048 DATGSI010000000
VERSION     DATGSI010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51022"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /gene="rplV"
                     /locus_tag="VK031_00745"
     CDS             <1..258
                     /gene="rplV"
                     /locus_tag="VK031_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HLS52475.1"
                     /translation="KQVDEALSILQFTPKKGARILEKVLASAIANAENNHNLDRDSLY
                     VSEAYANDGPHMKRWRPRAKGMASPILKRTSHIGVVVKERE"
     gene            276..941
                     /gene="rpsC"
                     /locus_tag="VK031_00750"
     CDS             276..941
                     /gene="rpsC"
                     /locus_tag="VK031_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HLS52476.1"
                     /translation="MGQKINPHGFRVGVIKDWESKWYADKNFDELLVEDYKIREHIKE
                     KLYVAGISGIKIERAANRIKLTIHTAKPGMVIGRGGSGVEALRKEIEKMTEKSVVINV
                     EEIKVPELDAQLVAENIASQLERRVSFRRAMKQAIQRTMRAGALGVKTSVSGRLGGAD
                     MARTEGYSEGTIPLQTLRADIDYGFCEADTTYGKLGVKVWIYKGEVLPTKKSDKEEKK
                     TGK"
     gene            968..1411
                     /gene="rplP"
                     /locus_tag="VK031_00755"
     CDS             968..1411
                     /gene="rplP"
                     /locus_tag="VK031_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HLS52477.1"
                     /translation="MLMPKRVKYRRVHRGRMKGQAHRGNTITYGEYGLQALEPTWITS
                     NQIEAARRAMTRYVRRGGNIWIKVFPDKPVTEKPAETRMGSGKGSPEYWVAVVKPGRI
                     LFEMGGVSEKDAREAMRLAAHKLSIKTKFVTRDEGQEKGGEANES"
     gene            1401..>1490
                     /gene="rpmC"
                     /locus_tag="VK031_00760"
     CDS             1401..>1490
                     /gene="rpmC"
                     /locus_tag="VK031_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HLS52478.1"
                     /translation="MKAKEIRQMSEQELDSKLLELKTELFNLRF"
BASE COUNT          559 a          205 c          363 g          363 t
ORIGIN      
        1 aaacaagttg acgaagctct atctatttta cagttcacac ctaaaaaggg tgcaagaatc
       61 cttgaaaagg tattggcttc tgcaattgca aatgctgaaa acaatcataa tttagataga
      121 gatagcctat atgtatcaga agcttatgca aatgatggcc cccatatgaa aagatggaga
      181 cctagggcta agggtatggc ttcaccaata ctgaaaagaa caagtcatat aggagttgtt
      241 gttaaagaac gtgagtagag aaggagggat agttaatggg tcaaaaaata aatccacatg
      301 gttttagagt tggagtaata aaggattggg aatccaaatg gtatgcagat aaaaactttg
      361 atgaactttt agtggaagac tataaaatcc gtgaacatat taaggagaag ttgtatgttg
      421 ctggaatatc tggcattaag atagaaagag ccgccaacag aattaaatta actatacaca
      481 cagccaaacc aggtatggtt ataggtagag gtggatctgg agtagaagct ttaagaaaag
      541 aaattgaaaa gatgacagag aaatctgtag taataaatgt agaagaaatt aaggtacctg
      601 agctagatgc tcaattggta gctgaaaata tagcctcaca actagaaaga agggtatcct
      661 tcagaagggc tatgaagcaa gctattcaaa gaacaatgag agcgggagct ttaggtgtta
      721 aaacttcagt ttcaggtagg ttaggcggag cagacatggc cagaactgaa ggttatagtg
      781 aagggactat accactgcaa acattaagag cagatataga ttatggtttc tgtgaagcgg
      841 ataccacata cggtaaacta ggagtaaaag tgtggatata caaaggagaa gttcttccta
      901 ctaaaaaatc agataaggaa gagaaaaaaa caggtaaata aaaactgtag gaaggaggaa
      961 ttttattatg ttaatgccta agagggtaaa atatcgtaga gttcatagag gtagaatgaa
     1021 aggtcaggcc caccgtggta atacaattac ttatggtgag tatggattac aagcattaga
     1081 accaacttgg atcacatcca accaaataga ggctgccaga cgtgctatga ctagatatgt
     1141 acgtagaggt ggtaatatct ggatcaaggt tttcccagat aaaccggtaa ctgaaaaacc
     1201 tgcagagact cgtatgggtt ctggtaaggg ttcccctgaa tactgggtgg cggttgttaa
     1261 accaggtaga attctatttg agatgggtgg agtaagcgag aaagatgcaa gagaggctat
     1321 gagattagca gctcataaat tatcaataaa aacgaaattc gtcactcgtg atgaagggca
     1381 agaaaagggt ggtgaggcta atgaaagcta aggaaattcg ccaaatgtcc gaacaagaat
     1441 tggacagtaa actattagaa ttaaaaactg aactattcaa tttgagattt
//
LOCUS       DATGSI010000049         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_522907, whole genome shotgun sequence.
ACCESSION   DATGSI010000049 DATGSI010000000
VERSION     DATGSI010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522907"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VK031_00765"
     CDS             <1..664
                     /locus_tag="VK031_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLS52479.1"
                     /translation="HPYGHERYESVFAKLLSILLLLTGAFIGYDSLKVLISGDIVEPK
                     IIALVAAGISVVVKEGMYHYTIRVAKKIKSLSMEADAWHHRSDAFSSIGTFMGILGAR
                     LGLVALDPIAGIIVSILVIKVGIDLYIKSIRELVDEAASDEVIAKIKKLTRATKGVRG
                     IKEVKTRNFGNRIYVDLEVYVDSHITVKEGHDIAEKVHDKLEYEIEDIKHCMVHIEPY
                     FE"
     gene            719..>1236
                     /locus_tag="VK031_00770"
     CDS             719..>1236
                     /locus_tag="VK031_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HLS52480.1"
                     /translation="MEGAIKGKKTSHKIMERVKGVVDELRRNGKQPGLGIIRLGEDPD
                     DLYYENNIKKNARKVGVKVKVMEKPCHITTDELIEEIERMNRDDTISGIIIFQPLPGH
                     INKDQVANSIDPLKDVDCIHPLNLMKIFQGDLTGFIPCTSKAVIDILIHNNVSLEGKK
                     VVIINRSLVVGKP"
BASE COUNT          435 a          152 c          276 g          373 t
ORIGIN      
        1 gcatccatat gggcatgaaa ggtatgaatc agtatttgct aaattgctta gtatattatt
       61 actattaact ggagcattta taggatatga ttctctaaag gtattaattt ctggagatat
      121 agtcgagcct aaaatcatag ccttagtagc tgctggaatt tctgtggtgg ttaaagaagg
      181 catgtatcac tataccataa gggttgctaa aaaaatcaag tccttgtcca tggaagctga
      241 tgcttggcat cataggtcgg atgcattttc ttctataggt acttttatgg gcatattagg
      301 tgccagacta ggcttggtag ccttggatcc tatagcaggt attattgtat ctattttggt
      361 cataaaagta gggatagatc tatatattaa gtctattagg gaattggtag atgaggcagc
      421 cagtgatgaa gttattgcta aaattaaaaa gttgaccaga gctaccaagg gtgtaagggg
      481 tattaaggaa gtgaagacta gaaactttgg taatagaata tatgttgacc tggaagttta
      541 cgtagattct catattactg ttaaggaagg ccacgatata gcagagaagg tacatgataa
      601 attggagtat gaaatagaag atataaaaca ttgtatggtt catatagaac cgtattttga
      661 ataaatttaa taaaaatggt aatcaattag taattttttt ttagatggag gtaatcctat
      721 ggaaggtgca attaaaggta aaaaaacatc acacaagatt atggaaaggg ttaaaggagt
      781 tgtagatgaa ttaagaagaa atggaaaaca acctggccta ggaattattc ggctggggga
      841 ggatccagat gatctatatt atgaaaataa tataaagaaa aatgcaagga aagtaggggt
      901 taaggttaag gttatggaaa agccttgcca tattacaaca gacgaactga ttgaagaaat
      961 tgaaaggatg aatagggatg ataccatatc aggtattata atcttccaac cattaccggg
     1021 acatattaat aaggaccagg ttgcaaactc aatagacccc ttaaaggatg tggactgtat
     1081 ccatccttta aatctaatga aaatattcca gggagatcta actggattta ttccttgcac
     1141 ctctaaggca gttattgata ttttaataca taataatgtt tctcttgagg gaaagaaagt
     1201 ggtaattata aatagatctt tggttgtagg gaaacc
//
LOCUS       DATGSI010000050         8292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_4950, whole genome shotgun sequence.
ACCESSION   DATGSI010000050 DATGSI010000000
VERSION     DATGSI010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4950"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VK031_00775"
     CDS             complement(<1..629)
                     /locus_tag="VK031_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLS52481.1"
                     /translation="MNRFRSLNFTLVVFVFLIMLFSSALTGGILLCLDKYNFFLEIKS
                     TKILLLISGLITSTIIGTLLTFPIGNYVLKPLNDLINGTREVSKGNYNIKVEELKMKH
                     EIGELIRSFNIMTKELSGIEMFRKDFINNFSHEFKTPIVSIRGFAKQLKNSKLDKEKQ
                     EEYIDIIIRESERLTNMSSNILLLTKLENQEIITDKKLYSLDEQIRNCIL"
     gene            complement(702..1376)
                     /locus_tag="VK031_00780"
     CDS             complement(702..1376)
                     /locus_tag="VK031_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLS52482.1"
                     /translation="MFNIMVVEDNLNTRKLMEAVLLENGYNPILAKDGIDALEILDRK
                     HIDLIILDIMMPNMDGYEFTKTLREGKCNIPILMVTAKEDISDKKKGFILGTDDYMVK
                     PVDEEEMILRIRALLRRAKIASEHRLIIGDTELRYDELSVKEGNQIFTLPQKEFMLLF
                     KLLSYQNKIFTRRQLMDEIWGPDSDSDKRTVDVHINRLRERFKNSKDFEIVTIRGLGY
                     KAVKLV"
     gene            complement(1413..2099)
                     /locus_tag="VK031_00785"
     CDS             complement(1413..2099)
                     /locus_tag="VK031_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HLS52483.1"
                     /translation="MFYDPTMIVLLPAIILTLYAQSKVQSSFNKYLRVPTEKGYSGVQ
                     VARLLLDQNGLQHIPIEYSRGRLTDHYDPKNKVLRLSEEVYKGTSIASVSVAAHEVGH
                     AIQDANDYVPLTFRNKVFPLARFGSSTAWFFIMAGLLIPSLGGLMDVGILLFATAVLF
                     QVIPLPVEFNASSRALELLDTNGFITHYENRGAKKVLRAAALTYVAAMASGISQLIRL
                     VLIRNRNNRN"
     gene            complement(2193..3044)
                     /locus_tag="VK031_00790"
     CDS             complement(2193..3044)
                     /locus_tag="VK031_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HLS52484.1"
                     /translation="MNKNKTILIIDGTNLIHRNYYVHSYRKTKSGIATGGLYGTIRSL
                     KSYINRFDPSQIYIAFDKSEHTFRKEVYPKYKANRKDIDSDLLSQFALLEKYCDLTNI
                     PFIQLDMYEADDIIASLSLHAKQYGLNPYVISGDKDLLQLMNKDVKIVYLSNSGPITY
                     SKEMFIEEYQIYPEQFIDYKALVGDPVDNISGIPGIGMKTAANLLTKFKNLDGIYKNI
                     DRIKGKRQENLLKYRDKVYLFKELLTLKPNLDLDYKKYFIEDVKAGFNLKNRDVQKFL
                     QELELKI"
     gene            complement(3383..4318)
                     /gene="guaA"
                     /locus_tag="VK031_00795"
                     /pseudo
     CDS             complement(3383..4318)
                     /gene="guaA"
                     /locus_tag="VK031_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002009981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
     gene            complement(4413..5864)
                     /gene="guaB"
                     /locus_tag="VK031_00800"
     CDS             complement(4413..5864)
                     /gene="guaB"
                     /locus_tag="VK031_00800"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HLS52485.1"
                     /translation="MEFVGEGLTFDDVLLVPQKSEILPRETDITTYLTKKIKLNIPLM
                     SAGMDTVTEARMAIAMAREGGIGIIHKNMSIEKQALEVDRVKRSEHGVITDPFYLSPD
                     HIVSDALELMERYHISGVPITNEDGKLVGIITNRDIRFEREVTKKIDDVMTKENLVTA
                     QVGISMDDALEAMKEHKIEKLPLVDDEFNLVGLITIKDIEKSIQHPNSAKDDSGRLLA
                     GAAVGVTGDMMDRVKALVEAKVDVIVVDTAHGHSKGVIDAVKRIKLEYPELQVIAGNV
                     ATAEATVDLIEAGADAVKVGIGPGSICTTRVVTGIGVPQITAVIDCAKAAKEYGVPVI
                     ADGGIKYSGDITKAIAAGANVIMAGSLFAGTDESPGEEVLYEGRRYKEYRGMGSEGAM
                     DSGSKDRYFQENTKKYVPEGIEGRIHYRGPLGDVVYQLIGGLRSGMGYVGAKSIEDLQ
                     TKAKFMKITPASLVENHPHNVYITKEAPNYSRR"
     gene            complement(5973..6416)
                     /locus_tag="VK031_00805"
     CDS             complement(5973..6416)
                     /locus_tag="VK031_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HLS52486.1"
                     /translation="MSGLIPFNRRGPRARMRGFEDFYGLLDDFFNEPRFMSPFRDTTF
                     RIDVKEKKDKYIVEAEVPGVSKKEINLRLEDGRLTIGIEREERKDEEEKDYIHKERRY
                     ASMSRSIYLGNVKEDKARAKLKDGILKIEVPKSQVDDTRRQIPID"
     gene            complement(6707..7066)
                     /locus_tag="VK031_00810"
     CDS             complement(6707..7066)
                     /locus_tag="VK031_00810"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HLS52487.1"
                     /translation="MKTKRVGTISMAIVLIGFGILLLIAQFSQISAVELAIKFWPLVL
                     IILGSEVLWFTYINKEGDNFLIRYDIFSMFIVLVILMVNIGLYGLMETGILDYIKLRV
                     SEETLIYERTLDELNNE"
     gene            complement(7056..7829)
                     /locus_tag="VK031_00815"
     CDS             complement(7056..7829)
                     /locus_tag="VK031_00815"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HLS52488.1"
                     /translation="MQRKSRLVTFLLSFIPGLSHFYIGYADRGIIYFLLFGVVTIGSV
                     GMGILTSSEIFLLTLIGGAALIWLVALIDAFNLIGRDRYIERGGGIEAELIEEESRKY
                     NRKIITLGLSIIPGAGHMYLGYQEKGLIYMGIFFFTIFFMGWLNLSFLLFLIPLIWFY
                     TFFDAFHIVNGNQVVEQEGYIFNLLPTINHEYIGKGLIGLGVMIILQKIFLPIVSIYV
                     NYRIRNIIQTSIVSLIFIFLGVRILQKNKHIEVEEEDED"
     gene            complement(7831..>8292)
                     /locus_tag="VK031_00820"
     CDS             complement(7831..>8292)
                     /locus_tag="VK031_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52489.1"
                     /translation="WLLYKNKLLSEEKQIEMENHLYTCDSCMDIFLSLINDDEIDKAA
                     TIVPENFTENILKNIGKIKPIRTKKSTKKGYRKSSDFFLYYAAVASVAIILTAGGVFG
                     KMVDLVPQISVNMSKKESQVKVDTIYNFSESITNRTSQFINNFGIKEEKED"
BASE COUNT         2416 a         1676 c         1089 g         3111 t
ORIGIN      
        1 aggatacagt ttctaatttg ctcatctaaa gagtatagtt ttttatctgt tattatctcc
       61 tgattttcta gcttggtaag taaaagtata tttgaagaca tattggtaag tctttccgat
      121 tctctgataa ttatatctat atattcctct tgtttttcct tatctaactt tgaatttttt
      181 agctgcttag caaaacctct aatggaaact ataggtgttt taaattcatg ggaaaaatta
      241 tttataaagt cctttctaaa catttctatt ccacttaatt ccttggtcat tatattgaaa
      301 cttctaatta attctcctat ttcgtgtttc atcttcagtt cttcaacctt tatattatag
      361 tttccttttg atacttctct tgttccatta atcaaatcat ttaatggttt aagcacataa
      421 ttccctatgg gaaaggtaag cagggtccct ataattgtgc tggttatcag gcctgatatt
      481 aatagaagta tttttgttga ttttatttct aaaaagaaat tgtatttatc tagacataaa
      541 agaatgcccc ctgttaaagc actggaaaac aacataatta aaaatacaaa aactaccaga
      601 gtaaaattta aacttctaaa tctattcatt ataatctccc accgtttttt ctttatctta
      661 tcacaaaatc cggtagacca cttaaggtct accggatata tttatactaa ttttacagcc
      721 ttatagccaa ggcctcttat tgtaactatt tcaaaatcct tgctattttt aaatctctcc
      781 ctaagcctgt ttatatggac atctacagtt cttttatctg aatcgctgtc tggcccccaa
      841 atttcatcca ttagttgcct tctagtaaaa atcttgtttt gataggaaag cagtttaaat
      901 aaaagcatga attctttttg tggtaaggta aatatctgat tcccttcttt tactgataat
      961 tcatcgtacc gtaattcagt atctcctatg atcaatctat gttcactggc tatttttgcc
     1021 cttcttaaaa gagcccttat ccttaagatc atttcttcct catctacagg tttcaccata
     1081 taatcatcgg tacctaagat aaagcctttc tttttatctg aaatatcttc cttggctgtt
     1141 accattagta taggtatatt acattttcct tctctaaggg ttttagtaaa ttcatatcca
     1201 tccatattag gcatcattat gtctaatatt attagatcta tatgcttcct gtctaaaatt
     1261 tctaaagcat ctattccgtc ctttgctagt attgggttat atccattttc caacaggact
     1321 gcttccatta gctttcttgt gtttagatta tcttctacta ccataatatt aaacataaat
     1381 gtccttccct ttccaaagag ttaactatac tcctaatttc tattgtttct atttctaatt
     1441 agaacaagcc taattaattg ggatattcca ctggccatgg cagctacata agttaaggct
     1501 gcagccctta gtaccttctt ggccccccta ttttcataat gggttataaa gccattggta
     1561 tctaataatt ctaaggccct actactggca ttaaactcta ctggcagagg aattacttga
     1621 aaaagtactg cagtagcaaa tagtaaaatt ccaacatcca ttagcccacc taaacttgga
     1681 attaaaagcc ctgccattat aaagaaccag gctgttgatg agccaaacct agccagaggg
     1741 aataccttgt ttctaaaggt taaaggtaca taatcattgg catcttgaat ggcatggcct
     1801 acctcatggg ctgccacact tactgatgct atagaggttc ctttgtagac ctcttctgat
     1861 aatctaagaa ccttattttt tggatcataa tgatccgtta gcctgcctct gctatattct
     1921 ataggaatat gttgtaatcc attttggtcc aaaagtagcc tggccacctg aactcctgag
     1981 taaccttttt ctgtaggtac ccttaaatat ttattaaagc tagattgaac tttactttgt
     2041 gcataaaggg tcaaaataat agctggtaat aaaacaatca ttgtgggatc ataaaacatt
     2101 tgtatcactt cctttataat aagttggtaa acttattata accatttaat attaactaaa
     2161 tattaacttt catagttaat ttataagttt tattagattt ttagctccaa ttcttgtaga
     2221 aatttttgaa catccctatt ttttagatta aacccagctt ttacatcttc aataaaatat
     2281 ttcttataat caaggtccaa gttaggctta agagtcaata gttctttaaa taaatatacc
     2341 ttatccctat atttaagtaa gttttcctgt cttttgccct ttattctgtc aatattttta
     2401 tatatgccat ctaagttttt aaattttgtc aacaaattgg ctgctgtttt cattcctatt
     2461 cctggtatac ctgatatatt gtctacagga tctcctacca gggccttata gtctataaat
     2521 tgctcaggat atatttggta ctcttctata aacatttctt ttgaataagt aataggtcca
     2581 ctgtttgata aataaactat tttaacatcc ttattcatta attgaagtag gtccttgtct
     2641 ccagatatta cataaggatt taatccatat tgttttgcat gtagggatag actggctata
     2701 atatcgtctg cttcatacat atccagttga ataaaaggaa tattggtcag gtcacaatac
     2761 ttttctagta aggcaaactg gcttaaaagg tctgaatcta tatcctttct attggcctta
     2821 tattttggat aaacttcctt tctaaaggta tgttcacttt tatcaaaggc tatataaatt
     2881 tgactgggat caaaccgatt tatataactt ttaaggcttc ttatggttcc ataaagacct
     2941 cctgtagcta ttcctgattt ggttttccta taggaatgga cataatagtt tctatgtatt
     3001 agattagtcc catcaattat taatatggtt ttatttttat tcaatgtcta ccttctttct
     3061 atatctttat acgcttttat taaaattaca ttgcctatat agctaaggat aatttaaaaa
     3121 tctattattt aattaaataa tatacatcca aattttatcc ctcatatgat atttattata
     3181 acatatatat ttttcctaat ttaagataag gctaattata aaaaataaaa cctttacccc
     3241 tagttaaagg agtcaaggtt tttacctact ctagccgcag gttgtggact tctagctccc
     3301 tttaatccct acaagcctcg ccaaccctaa tagggtagta cctacaaagt tttctacgcc
     3361 ttcagcttga aagctatggg tgctactccc actcaatagt tgctggtggt ttgcctgtta
     3421 tgtcatatac taccctattt acttcctcca cttcattggt aattcttgtg gatatctttt
     3481 ctaatacttc caaaggtatt ggataccatt ggccctccat accatcttca gaggtgacgg
     3541 cccttaaggc tatagtatgg ctataggttc ttttcccgtc tataactcct actgattgga
     3601 tattggggag aactgcaaag tattgccaaa tattgagtcc agcttttttt atctcttccc
     3661 tgaaaatata atctgcctcc tttagaatct ggatcttttc tttggttacc tctcccaaaa
     3721 tcctaatacc taggccaggt cctgggaatg gctgcctatc tacaatctca gatgatattc
     3781 ctaatagtct accaacttcc cttacctctt ccttatatag gtctcttaag ggttctagga
     3841 ttttaaaatt tacatcatca ggtaggccgc ctacattatg atggctttta actgccaatt
     3901 tccccttagt acctgactca ataatatctg attttatggt gccttggatt aaaaaatcta
     3961 tattttcaag ttttgactgc tcctcttcaa agaccctaat aaattcttcc cctataatct
     4021 ttctttttga ttcagggtct gtaacccctt ttaatttatt taaaaatcta tcctccgcgt
     4081 ccactacaat cagattcatt tgaaattcat ctttgaaaat cttttctact tcttcccttt
     4141 cctttttcct aagtaggcca tgatctacaa atacacaagt aagcctatct cctatggcct
     4201 tgtgcactag tactgcagcc acagaagagt ctactccacc tgatagggca catagagctt
     4261 ttttattacc tattttctct ttaatttctt ctatactttt atctatgaaa gtttgcatct
     4321 tttcctccta ctttctctat ttcttgcaag tttctagtat taaaagaaaa cgacaccaag
     4381 gtcgtttcca tatagactta aaatataaaa ttttatctcc ttgaatagtt aggagcctct
     4441 ttagttatat aaacattatg gggatggttt tctactaggc tggctggtgt tattttcata
     4501 aatttagcct ttgtttgtaa atcttctatg cttttggctc ccacatagcc cattccagat
     4561 cttaggccac ctattaattg gtagactaca tctcccaagg gtcccctata atgaatcctt
     4621 ccttcaattc cctcaggtac gtacttttta gtattttcct ggaaatatct atccttgctt
     4681 ccagaatcca tggccccctc agagcccatg cccctatatt ctttgtatct tctaccctca
     4741 tataggactt cttcgcctgg actttcatca gttcctgcaa aaagagaacc tgccatgatt
     4801 acattagctc cagccgctat agccttggtt atatcaccag aatatttaat accaccgtca
     4861 gcaattactg gaactccata ttctttggct gctttagcac aatcaattac ggcggttatt
     4921 tgtggaactc caatacctgt aacaaccctg gtagtacaaa tagagcccgg tcctatacct
     4981 accttaacag catctgcacc tgcctctatt aaatctacag ttgcctcagc cgttgccaca
     5041 ttacctgcta ttacttgaag ctctggatat tccagcttaa ttctttttac agcatctata
     5101 acacccttag aatgtccatg ggctgtatct acaactatca catcaacctt agcctctact
     5161 aaagccttta cacgatccat catatctcca gttacaccta cagctgcacc agctaataat
     5221 cttcctgagt catccttagc agaatttgga tgttggattg atttttctat atcttttatg
     5281 gttattaatc ctaccaaatt aaattcatca tctactaagg gtagtttttc aatcttgtgt
     5341 tccttcattg cttctaaggc gtcatccatg gagataccca cttgggctgt tactagattt
     5401 tcctttgtca ttacatcatc tatcttttta gttacttctc tttcaaatct aatatccctg
     5461 ttagttatta tacctactag tttcccatct tcattggtta taggtacgcc tgatatatga
     5521 tatctctcca ttaattctaa agcatctgat actatatggt ctggggatag ataaaaagga
     5581 tctgtaatta ctccatgctc cgatcttttt accctatcca cctctaaagc ttgtttttct
     5641 atggacatat ttttatgaat tatacctata ccaccttctc tggccatggc tatagccatt
     5701 ctggcttcag taactgtatc catgccagca ctcattaggg gtatatttaa tttaattttt
     5761 ttagttaaat atgtggttat atcagtttcc cttggtaaaa tttctgattt ttggggaacc
     5821 aataaaacat catcaaatgt taatccttca cctacaaatt ccaagtctat ccctcctata
     5881 cttttaaata gtatatcata aataattttt tttcaacatg aaatatttat tttctattaa
     5941 aaaaagatag ggtcgaaccc tatcttttaa gcctagtcta ttggtatctg tcttcttgta
     6001 tcatctactt ggcttttagg aacctcaatt tttaatatac catcctttaa tttagccctg
     6061 gccttatctt ctttaacgtt gcctaaatat atacttctac tcatagaggc atatcttctc
     6121 tccttgtgga tatagtcttt ttcttcttca tcttttcttt cttctctttc aattcctatt
     6181 gttagacggc catcttctaa ccttaaattt atctcctttt tgctaacgcc aggtacttcg
     6241 gcttccacta tatatttgtc tttcttttct ttaacgtcaa ttctaaaagt agtatctcta
     6301 aaaggactca tgaaccttgg ttcattgaag aagtcatcta aaaggccata aaagtcttca
     6361 aagcctctca ttcttgctct aggacctctc ctattaaatg gtattaatcc tgacatagga
     6421 tcatctcctt tacttttaga ttttgttatt agcagtctaa gactttggct gctaataata
     6481 ttattacata gtattaagaa cctgtcaaga ttaacttgat ttacatttag ttttgccaat
     6541 tcctaaccta atatgtaaat aattatattg attccccctc tatcctatag gtgaatagtc
     6601 tataggcaaa tacattaatt aacaatcctg aaataggtgc ttccaatgag gccaagagag
     6661 atattgaagc ctttttatgt caagaaaaaa gaacctttcg gttcttttat tcattgttta
     6721 gctcatctaa agttctttca tatattaaag tctcttcact tactctcaat tttatataat
     6781 ctagtattcc agtttccatt aatccatata gcccaatatt gaccataagg attaccagta
     6841 caataaacat ggaaaatata tcatatctta taagaaaatt gtccccttcc ttatttatat
     6901 aggtaaacca aaggacttca ctacctaata ttattaacac tagaggccaa aacttaattg
     6961 ccaattctac tgcagatatt tgactaaact gggctattaa taatagtatt ccaaagccta
     7021 ttaaaactat ggccatagat atggtaccta ctcttttagt cttcatcttc ctcctccact
     7081 tctatatgct tatttttttg tagtatccta acacctaaga atataaatat taaagataca
     7141 atagaggttt gtataatatt tcttattcta taatttacat atattgatac tataggtaaa
     7201 aagattttct gtagtataat cattacacct aagcctataa ggcctttccc aatatattca
     7261 tggtttattg tgggaagtaa attaaatata tagccttctt gttctacaac ctgatttcca
     7321 tttactatat ggaaggcgtc aaagaaggta taaaaccaaa ttaagggaat tagaaataat
     7381 agaaagctta aattaagcca gcccataaag aatatagtga agaaaaatat ccccatatat
     7441 attaagcctt tttcctgata tcccaaatac atatggcctg cacctgggat tatggatagt
     7501 cctaaggtga ttatttttct attgtatttt ctactttcct cctctatcag ctctgcctca
     7561 attcccccac ccctttcaat gtatctatca cgacctataa gattaaaagc atctatcaag
     7621 gctaccagcc atatgagggc tgcacctccg attaatgtta ataaaaaaat ctcactacta
     7681 gttaaaattc ccatacctac tgaacctata gttaccacac caaatagcag aaaatatata
     7741 attcccctat ctgcatagcc tatataaaaa tgggataatc ctggtataaa agataataaa
     7801 aatgtaacaa gcctactttt tctctgcata ctaatcctcc ttttcttcct taattccaaa
     7861 attatttata aattgtgagg ttctatttgt aattgattct gaaaaattat atattgtatc
     7921 tacttttacc tgactttctt ttttagacat atttacactt atttgaggta ctaagtctac
     7981 catttttcca aatactccac cagctgttaa aataatagcc acagatgcta ccgcagcata
     8041 atataggaaa aagtcactac tttttctata gcctttcttt gtgcttttct tagttcttat
     8101 aggttttatt tttcctatat tttttaaaat attttctgtg aagttttcgg gtacaatggt
     8161 agctgccttg tctatttcat catcatttat taaggataaa aatatgtcca tacaagaatc
     8221 acaagtgtat agatgatttt ccatttctat ttgcttttct tcacttaata acttattttt
     8281 ataaagtagc ca
//
LOCUS       DATGSI010000051         4134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_617843, whole genome shotgun sequence.
ACCESSION   DATGSI010000051 DATGSI010000000
VERSION     DATGSI010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617843"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /locus_tag="VK031_00825"
     CDS             <1..1709
                     /locus_tag="VK031_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS52490.1"
                     /translation="NEAGIETEELGLQVANWDNDKANQAMEAWLAKDGDRIEFVIANN
                     DGMASGAVSALQNAGYNTGDPEKYIPVFGVDATEEAINLIERGAMTGTVKQDGEGMAK
                     AVHALMGEDGAGKSTLMKILFGIYKEDAGEIYIDGKKTIFPNPRVALEKGVSMVHQEL
                     NQVLQRNVMDNIMLGRYPQRGIFIDHRAMYKFTRDIFNDLGIDIDPRTKLSNLSVSER
                     QMVEIAKAVSYDAKILVLDEPTSSLTEQEVEHLFKIINLLRDRGCGIVYISHKMEEIL
                     RISDEVTVLRDGKWVATECAKDLTTDKIISLMVGRDLTNRFPDKSNEPGEVMLEVKNL
                     TGKYQPSVRNAFFQLRKGEILGVAGLVGSRRTELLETIFGIASRSSGEIYLNGELIEN
                     KDPTVAVKNGFALLTEERRATGIFGELNVRFNSMIANMDNYSKYGVINNKKTREDTEW
                     VIDNLNVRTPSQDTLIKSLSGGNQQKVVIGRWLLTDPEVLLLDEPTRGVDVGAKYEIY
                     QLIIDLAKRGKGVIFVSSEMPELLGICDRILIMSNGRIAGIEEAKNLDQEKIMSLAAK
                     YL"
     gene            1735..2643
                     /locus_tag="VK031_00830"
     CDS             1735..2643
                     /locus_tag="VK031_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004836672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-methylgalactoside transporter"
                     /protein_id="HLS52491.1"
                     /translation="MGSIRKYTKNISKEYIVAGLLDYALDIILCLMILGVIIIDPSFV
                     SIRNFTNILSQASTRAILGMGVAGLIVLQGTDLSAGRILGLSGIVAASLLQDLNYASR
                     MYPNLQELPMIVPFMIAVAIGVTISAINGFGVAKLNLHAFIVTLGTQLIAYGLALIYI
                     DRPPLGAQPIAGLDKRYTELVKGSFNIGIGSIRFQLPYLIIYAAIIAIIMWFIWNKTK
                     LGKNMFAIGQNYELDAIAACVVGGVSFSGGVGTIPGVLIGAVLLQFINYGLNFIGVNP
                     YLQIIIKGIIIIVAVSIDVRKYIEKK"
     gene            2668..3669
                     /locus_tag="VK031_00835"
     CDS             2668..3669
                     /locus_tag="VK031_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLS52492.1"
                     /translation="MYDVAKKAGVSIATVSRAINKNYPVKEETRKKIEEAIEELGYIP
                     NEIARSLTLKSTSTIGVIVPSITNLFFPTMVEEINKVLINKGFTTSLYSTEGNPNLEK
                     TVAENIISRSMDGIIAIDPSIENLENNYFKNLSQKIPTIIINGDTDKYQCNFVSYDEK
                     AGAKEAFRYLLNLGHREISFIRGDRSLSYDIKEDVYRSFIKENNLKYKKIINVGMGNN
                     LEVIENTKRICEQLLISRKIGTAVFACNDLMAVGVINACNKVKVRVPQDISVIGFDNI
                     LLSNIVYPPITTIDLNIEEVSKKAARELIKMIKEKSTEMEKRVYKTKLIIRESCSER"
BASE COUNT         1568 a          481 c          894 g         1191 t
ORIGIN      
        1 taaatgaagc aggtatagaa acagaagagt taggacttca agttgcaaac tgggataatg
       61 ataaggcaaa tcaggccatg gaagcatggt tagctaaaga tggagacaga attgagtttg
      121 ttatagctaa taatgatggg atggcttcag gagcagtgtc tgcactacaa aatgcaggat
      181 ataataccgg ggatccggaa aaatatatac cagtatttgg agtagacgct acagaagaag
      241 ccataaactt aattgaaaga ggtgcaatga caggaacggt taaacaagat ggagagggaa
      301 tggcaaaggc tgtacatgca ttaatgggag aagatggagc tggaaaatcc acactaatga
      361 aaattctttt tggaatatat aaagaagatg ctggtgaaat atatattgat ggtaaaaaga
      421 ctatattccc caatcctaga gtggcgttag aaaaaggcgt gtctatggta catcaagaat
      481 taaatcaagt gctccaaagg aatgtaatgg acaatattat gctaggtaga tatccgcaga
      541 gaggtatatt catagatcat agagctatgt ataagtttac cagggatata tttaatgatc
      601 taggcattga catagatcct agaaccaaat tatctaatct ttctgtttca gagaggcaaa
      661 tggttgaaat agccaaggca gtatcctatg atgctaaaat attagtgttg gatgagccta
      721 catcatcttt aactgagcaa gaggttgagc atctatttaa gataataaat ttgcttagag
      781 atagaggatg tgggatagtc tatatatctc ataaaatgga ggaaatttta agaatatcag
      841 atgaggtaac agtacttagg gatggaaaat gggtagcaac agaatgtgca aaagatttaa
      901 ctactgataa aattataagt cttatggtgg gaagagattt gacaaatagg tttccggata
      961 agagtaatga gcctggagaa gttatgttag aggtaaaaaa tctaactgga aaatatcaac
     1021 cctcagttag aaatgcattc ttccaattaa gaaagggaga aattttagga gttgctgggc
     1081 tagtgggctc aagacgaaca gaacttttag aaactatttt tgggattgca tctcgttcct
     1141 ctggagagat atatctgaat ggagaattaa tagagaataa ggatccaaca gtagctgtaa
     1201 aaaatggatt tgctttatta actgaagaaa gaagagctac gggaattttc ggagaactaa
     1261 atgtaagatt taattctatg atagctaaca tggataatta ttctaaatat ggagtaataa
     1321 ataataaaaa gactagagag gatacagaat gggttataga taatctaaat gtacggacgc
     1381 caagccagga tactttaatc aagtctttat ctggtggtaa tcagcagaaa gtagttatag
     1441 gcagatggtt attaacagat ccagaagttt tacttttaga tgagccaacg agaggtgtag
     1501 atgtaggggc aaaatatgaa atatatcaac ttataattga tttggccaag agaggaaagg
     1561 gtgtgatttt tgtttcctca gagatgccgg agttattggg aatttgtgat aggatattga
     1621 ttatgtccaa tggaaggata gcaggcatag aagaggctaa gaatttagat caggaaaaga
     1681 tcatgagttt agcagcaaaa tacctataaa gatagaatgt aaggggtgta tgaaatggga
     1741 agtattagaa aatatacaaa aaatataagt aaagaatata tagtggcggg gctattagac
     1801 tatgctcttg atattatatt atgcctaatg atactaggtg ttataattat agatccatct
     1861 tttgtatcca taagaaactt tacaaatatc ttaagtcagg cctcaaccag ggctatactg
     1921 ggtatgggag tagcagggtt aatagtattg caaggaaccg acttatccgc aggaagaata
     1981 ctgggtcttt cgggtattgt tgccgcttct ttgttacaag acctgaacta tgcatctaga
     2041 atgtatccta atttacaaga attgcctatg atagtcccat tcatgattgc tgtagccata
     2101 ggggtaacta tatcagctat aaatggattt ggagtagcaa aattaaactt acatgcattt
     2161 atagtaactt tgggtacaca gcttatagct tatgggttag cattaatata tatagataga
     2221 ccaccattag gggctcagcc aattgctggg ctagataaaa ggtatactga gcttgttaag
     2281 ggctctttta atatcggcat agggagtatt aggtttcaat taccatattt aataatctat
     2341 gcggcgatta tagctattat aatgtggttc atatggaata agacaaaact aggcaaaaat
     2401 atgtttgcga tagggcaaaa ttatgagtta gatgcaatag cagcatgtgt tgtaggagga
     2461 gtatcatttt cgggcggagt aggaacaatt ccaggtgtat tgataggagc agtattatta
     2521 caatttatta attacggttt aaactttatt ggcgtaaatc catatttaca aataattatt
     2581 aaaggtatta ttataatcgt agcggtatct atagatgtaa ggaagtatat tgagaagaaa
     2641 taaagggggt gattttttat aataactata tatgatgtag caaagaaagc aggagtatcc
     2701 atagccaccg tttcaagggc aataaataaa aattatcctg ttaaagaaga aacaagaaag
     2761 aaaatagaag aagctataga ggaattggga tatataccta atgaaatagc tagaagttta
     2821 actcttaaaa gcacctctac cattggtgta atagtcccta gtattacgaa cctgtttttt
     2881 cctaccatgg tagaggagat aaataaagta ttaatcaata aaggatttac aacttcacta
     2941 tatagtactg aaggaaatcc caatttagaa aaaacagtag cagagaatat tatttctaga
     3001 agtatggatg ggattatagc tattgatcca tcaatagaaa acttagaaaa taattatttt
     3061 aagaatttaa gccaaaaaat tcctacaatt ataatcaatg gtgatacaga taagtatcaa
     3121 tgtaatttcg tttcttatga tgaaaaagcg ggggctaagg aggcctttcg gtatttatta
     3181 aacttaggac acagagaaat aagctttatt cgtggagata ggagtttatc ctatgatatt
     3241 aaagaagacg tttatagaag cttcataaag gaaaacaatt taaaatataa aaaaataata
     3301 aatgtaggaa tgggaaataa tctagaggta attgaaaata caaagagaat ttgtgaacaa
     3361 cttttgattt caagaaaaat aggaacagca gtatttgcat gtaatgattt aatggcagta
     3421 ggagttataa atgcttgtaa caaggtaaag gtaagggtac cacaagacat atcggtgata
     3481 ggttttgaca atatactgct atctaatatt gtatatcctc caataactac aatagattta
     3541 aatatagaag aagtaagtaa aaaggcagct agggaattaa taaaaatgat aaaagaaaaa
     3601 agcacagaaa tggaaaaaag ggtatataag actaaattaa taataaggga aagttgtagc
     3661 gaaaggtaga cgacagaaat aggttaaaag caaataagga agtgtccaaa tataaggacc
     3721 atagtgataa aaatttatag tgtgcataat atatatgact agaaataagg agtggttatt
     3781 ctgactaagg aaagacaaag atgatttacc aaattacggt gatgaacttg aggtacggat
     3841 ggaatgatta aactaccaat agatgcaaat accttactgg caaattcaag gtgctatatt
     3901 gttggatcgt agtggtatgt aaggataatt tgggaaatag aatttataag aaaaaggctc
     3961 ttggttaata aagggtttaa aagcctacca tttaaattta ctaagagtgt gtaattaaag
     4021 aatattaata ttacttatgc ggactttgaa gttttaaatg gagacaacat agatattaat
     4081 tatgtttagg atccctatgt aaatattttg agatatttat acaaaaccta tttt
//
LOCUS       DATGSI010000052         3436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_146300, whole genome shotgun sequence.
ACCESSION   DATGSI010000052 DATGSI010000000
VERSION     DATGSI010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146300"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VK031_00840"
     CDS             complement(<1..1304)
                     /locus_tag="VK031_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HLS52493.1"
                     /translation="MKFWVDFKKGRWTETVDVRDFIQLNYTPYEGDAEFLAPVSDRTK
                     DLWDKADQMIKDEILTGEVNIDTTRFSGINNYGPGYLDKENELIVGFQTDEPLKRIVN
                     PYGGYRMVQNSLKAYGKEMDETAEKHFNEYRKTHNQGVFDAYTTEMRAARSAGLLTGL
                     PDAYGRGRIIGDYRRVALYGIDFLKEEKLKDKENLKGNATEDLIRLREELTEQIRALD
                     EIKEMAASYGIDISKPASNAHEAVQFLYLGYLAAIKENNGAAMSLGRNTTFLDIYIER
                     DLKKGIITETEAQELIDQFVIKLRMARHLRTPEYNELFAGDPTWVTESIGGMGLDGRT
                     LVTKTAFRFLHTLTNLGPAPEPNMTVLWSADLPQGFKEYCAKMSIETGSIQYENDDLT
                     RPIYGDDYAIACCVSAMQVGKEMQFFGARANLAKALLYAINGG"
     gene            complement(1455..2789)
                     /locus_tag="VK031_00845"
     CDS             complement(1455..2789)
                     /locus_tag="VK031_00845"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(p)ppGpp synthetase"
                     /protein_id="HLS52494.1"
                     /translation="MKLELFDFIDETMNLIDEKSDMLNKVAKELERFFNNSFFIKDHF
                     LNVNYRIKSPESLREKILRYNFYLKYKTPEKLFKNLSDLIGFRIECRFIEDEAKIYKD
                     IRKLFDTPVGNGYYSNPLNSSILLNLDEKQPQIQKNGFEIYKIDGKYKKDDVEVNFEL
                     QIKSLVNVFWGEIDHRVLYKNFNYMLTEDFFRDIMSSIKDNLAMIDRQLMVVYNHLNG
                     MDTSDALNKEMQLKSLLSKIIHDIYITKVRQEIGFIVDFKKSTDVIVDYIFMKNGPGK
                     TINYSEDFLRILNRLDEIANEEISFKKYIDFEREIFFNDCFTRKIGTKILEVINKDFR
                     WNLFFKIIFDIEDGDNATDFEGFLIYLRYLFYDSIMAEINEKDMTVEEKEKIAETIMD
                     AIAESFNKSLDIDFINTCSINKLKICLRKHFKNIKTYDHWLDRKDRLIKEII"
     gene            complement(3036..>3436)
                     /locus_tag="VK031_00850"
     CDS             complement(3036..>3436)
                     /locus_tag="VK031_00850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLS52495.1"
                     /translation="ASHILVDTMEEAQKVMEEINNGLTFEEAAGKYSTCPSKENGGDL
                     GQFSRGQMVPEFEEEAFKMEEDSISEPVKTQFGYHIINLKEKDAGGLNSFDEVKNQIH
                     ENLLRLKQQEKYLNKIDQLKNEYKVEYVNN"
BASE COUNT          992 a          695 c          427 g         1322 t
ORIGIN      
        1 cctccattga tggcataaag tagtgctttg gccaaattgg ctctagctcc aaagaactgc
       61 atctcttttc ctacttgcat tgctgaaaca cagcaggcta tggcataatc atcaccgtat
      121 ataggtctcg ttaggtcatc attttcatat tggattgatc cagtttctat tgacattttt
      181 gcacaatatt ccttaaagcc ctgtggtaga tctgctgacc ataaaactgt catatttggc
      241 tctggtgcag gtcctaggtt tgtcaaggtg tgtagaaatc tgaaggctgt tttagtaact
      301 agggttcttc catctaggcc catgcctcct attgattcag taacccaggt tggatctcca
      361 gcaaacaatt cattgtattc tggagttctc aagtgtctag ccattcttaa tttgattaca
      421 aattggtcta taagttcttg ggcctcagtt tctgtaataa ttcccttctt taagtccctt
      481 tcaatgtata tatctaaaaa tgttgtattt ctacctagag acatggccgc cccattattt
      541 tcctttatgg ctgctaaata tccaaggtat aggaactgaa ctgcctcatg ggcattgctg
      601 gctggcttag aaatatctat tccataggat gctgccatct ccttaatctc atctagagcc
      661 cttatttgct cagttaattc ctccctaagc ctgataaggt cttctgttgc atttcctttt
      721 agattttctt tatcctttaa cttttcttcc tttaaaaagt ctataccata taaagctacc
      781 cttctgtaat cacctattat ccttcccctt ccataggcgt caggtaaacc tgttaatagt
      841 ccagcacttc tggcagcccg catctctgtt gtatatgcat caaagacgcc ttggttatgt
      901 gtttttctat attcattaaa gtgtttttct gctgtctcat ccatttcttt tccatatgct
      961 tttaatgaat tttgaaccat tctatacccg ccatatggat ttactattct ttttaatggt
     1021 tcatctgtct gaaatcctac aattaactca ttttccttat ctaaatatcc tgggccataa
     1081 ttgttaatac cagaaaatct tgtagtgtct atattaactt cacctgttaa aatttcatcc
     1141 tttatcatct ggtctgcctt atcccataag tcctttgtcc tatctgaaac tggcgctaga
     1201 aattctgcat ctccttcata aggtgtataa tttaattgga taaaatccct tacatccaca
     1261 gtttctgtcc atcttccttt tttaaaatcc acccaaaatt tcatattatc tcctccaatt
     1321 tatatataaa agttaagtaa atttctatct tgacttaatt atacaaacta aaggaaaata
     1381 atgcagtgat gtgcatcaca gttttcaaaa aaaataaaaa gtatttggtt tcccaaatac
     1441 ttttaaggct tttattaaat aatttcttta attaacctat cctttctatc cagccagtga
     1501 tcataggttt taatattttt aaagtgtttt ctcaaacaaa tctttaactt atttatacta
     1561 catgtattta taaaatcaat gtctaaactt ttattaaagc tttcagcaat agcatccata
     1621 atagtttctg ctatcttttc cttttcctct acagtcatat ccttctcatt tatctccgcc
     1681 ataatactat catagaaaag atatctaaga tatattaaaa atccttcaaa atctgtagca
     1741 ttatctccat cttctatatc aaagattatt ttaaagaaaa ggttccatcg aaaatcctta
     1801 tttatgactt ctaaaatttt cgtccctatt ttcctggtga aacagtcatt gaaaaagatt
     1861 tctctttcaa aatcaatata ttttttaaaa ctgatctcct cattggctat ttcatccaaa
     1921 cgatttaata ttcttagaaa atcttcacta taatttatag tcttgccagg cccattcttc
     1981 ataaatatat aatctactat aacatcggtt gactttttaa aatcgactat gaaaccgatt
     2041 tcctgcctta ctttagtaat gtatatatca tgaattatct tagataagag agattttaac
     2101 tgcatttcct tatttaaagc atcgctagta tccatcccgt taaggtgatt ataaaccacc
     2161 attaattgtc tatctatcat ggccaggttg tctttgatgg aggacataat atccctgaag
     2221 aaatcttctg ttaacatata attaaagttt ttatacaaaa ccctatgatc aatttctccc
     2281 cagaaaacat ttaccaggga ctttatctgt aattcaaaat ttacttccac atcatctttt
     2341 ttgtattttc catcaatctt ataaatttca aaaccgtttt tttggatttg tggttgtttc
     2401 tcatctaaat ttagtaatat gcttgagttt aaagggttag agtagtaacc attgcctact
     2461 ggtgtatcaa ataatttcct tatatcttta taaatttttg cctcgtcctc aataaaccta
     2521 cattcaattc taaagccaat cagatcagat aagtttttaa ataacttttc aggtgtctta
     2581 tatttaagat aaaaattata tctaagaatc ttttctctta aactttctgg agactttatc
     2641 ctgtagttta catttaaaaa gtgatcttta ataaaaaagc tattattgaa aaatctttct
     2701 aattctttag caaccttatt taacatatca gacttctcgt ctattaggtt catagtttca
     2761 tctataaagt cgaataattc taatttcata gtggtccccc ttagatcttc taacctaatt
     2821 ataccacaaa gcctaattta cttggttaaa aatacctttc aatttacgac aaaaatcttc
     2881 caccaattat tgagataaac ctttaaatat caaatttaaa agcccatccc tttaatagat
     2941 accagatttt gaaaaaatta ttcatagcct aaacttattt tcataatatt tgcacttttc
     3001 aacttagtga aaaggagagg tttctctccc tccccttaat tatttacata ttctaccttg
     3061 tattcatttt ttaactgatc aatcttgttt aggtatttct cctgctgttt taatcttaat
     3121 aggttttcat ggatctgatt ctttacctca tcgaagctgt ttaatccacc ggcgtctttt
     3181 tcctttaggt ttataatgtg atatccaaat tgtgttttca ccggctctga tatgctgtct
     3241 tcttccattt taaaggcttc ttcctcaaat tctggtacca tttgacctcg tgaaaactgc
     3301 cctaaatctc caccattttc cttagatgga caagttgaat atttgccggc cgcctcttca
     3361 aaagttaagc cattatttat ttcttccatc actttttggg cttcttccat tgtatctact
     3421 aatatatggc ttgccc
//
LOCUS       DATGSI010000053         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_193318, whole genome shotgun sequence.
ACCESSION   DATGSI010000053 DATGSI010000000
VERSION     DATGSI010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193318"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VK031_00855"
     CDS             <1..265
                     /locus_tag="VK031_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HLS52496.1"
                     /translation="NQTGYGNGQTYMDPAIEFKNKYEKSCDLLGVPYDADKYQIKLAY
                     RKKAKKYHPDLNKAPDATEKFQRINDAYEFLSDANIERYKTLR"
     gene            290..1657
                     /gene="rlmD"
                     /locus_tag="VK031_00860"
     CDS             290..1657
                     /gene="rlmD"
                     /locus_tag="VK031_00860"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HLS52497.1"
                     /translation="MEVFKIIKQDNLIKGEIIDFSHDGKGVLKVDNFTIFAYGGLIGD
                     IVEVKIEEIKRNYATGRVVRIIKPSKDRCQVDFQVDESKGAIPLIEYDYLKQLDWKQE
                     KVRKDLERIAGLDNVQVEKTIGMDTPFRYRNHTQIPVGKQKGRLEIGFYEINSRNIVD
                     MEGSIIQPKIGDEILQLIREWMKKFNISPYDRQKQKGLIRHIGIRTNKDGEAMVILVT
                     SNHKLPNKKELIEELTKTNVVSIYQNINRKNWSVTYGKEYIKLYGQEKLTDYIGKFKF
                     NLSPNSFFQVNRPQAEVLYNKVVEYLDLDQEDIVYDLYSGIGTISLFIANKVKKIYGI
                     EMVKAAVEDAWENAELNGVDNVEFITGRAEKVFPALLRKGIEANKLILDPPRKGCEKE
                     VLEAIIELESEKIAYVSCNPSTMARDVKYLVHNGYKVKEIQPVDLFPHTAHVETVVLM
                     SRIEN"
     gene            1993..>2341
                     /locus_tag="VK031_00865"
     CDS             1993..>2341
                     /locus_tag="VK031_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52498.1"
                     /translation="MKNKKRILLIAIAFLILALTACTNGKEENKSSVGETDKSQQEIN
                     NEDKVDSTDDYENKPNFFIGTIEEINGEVALVSIEKGEILKSGSKVGVDLSVANDKTF
                     QIGNKVKVGYDGII"
BASE COUNT          919 a          275 c          485 g          662 t
ORIGIN      
        1 aaaccagaca ggatatggca atgggcaaac atatatggat cctgccatag aatttaaaaa
       61 taaatatgaa aaaagttgtg acttattagg tgttccatat gatgcagata aatatcaaat
      121 aaaactggcc tacaggaaaa aggctaaaaa gtatcatcca gatttaaaca aggcccctga
      181 tgctacagaa aagtttcaaa ggataaatga tgcctatgaa tttttaagtg acgctaatat
      241 agaaaggtat aaaactttaa gataattaaa tggattacag ataatttata tggaggtttt
      301 taagattatt aagcaggata atttgattaa gggagaaata atagattttt cccatgatgg
      361 gaagggagta ttgaaggtag ataattttac catatttgcc tacggtggtt taattgggga
      421 catagtagag gtaaaaatag aagagataaa aagaaactat gccacaggca gggtagttag
      481 aattattaag ccttctaagg ataggtgcca agtggatttt caagtggatg aatctaaagg
      541 ggccattcct ttaatagaat atgattatct caagcaattg gattggaagc aagaaaaggt
      601 tagaaaagat ttggaaagaa ttgcagggtt agataatgta caggttgaaa aaaccatagg
      661 catggacacc ccctttagat acagaaacca tacccaaatt ccagttggta aacagaaagg
      721 taggctggaa ataggcttct atgagataaa cagcagaaat atagtagata tggaaggtag
      781 tataatccag cccaagattg gagatgaaat acttcagctt attagagagt ggatgaaaaa
      841 atttaatata tcaccttatg ataggcagaa acaaaaaggc ctaataagac atataggcat
      901 tagaacaaat aaagatggag aagccatggt tatactagta acttccaatc acaaattacc
      961 aaacaaaaag gaattaattg aagaactcac taagactaat gtagttagta tatatcaaaa
     1021 cattaatagg aagaattggt cagtaacata tggaaaagaa tatataaagc tttatggtca
     1081 agaaaagcta accgattata ttggaaagtt taagtttaac ttatcaccca attccttttt
     1141 tcaagtaaac aggccccagg cagaagttct ttacaacaag gttgtagagt atctagacct
     1201 tgaccaggaa gatattgttt atgatctata ttcaggtatt ggaaccatat ccttatttat
     1261 agcaaataaa gttaaaaaga tatatgggat agaaatggta aaggcagctg tggaagatgc
     1321 ttgggaaaat gcagaattaa atggagtaga caatgtagag tttataacag gaagggcgga
     1381 aaaggtcttt ccagcactac ttagaaaagg gattgaagct aacaagctaa tcctagatcc
     1441 gccaagaaag ggttgtgaaa aagaagtctt agaagcaata atagaattag agtctgaaaa
     1501 aatagcctat gtatcctgca atccttctac catggcaagg gatgtaaaat atttagtgca
     1561 taatggatat aaggttaagg agatacaacc ggtagatctt tttccacata cggctcatgt
     1621 tgagacggta gtattgatgt ctaggataga aaattaaaat atagttaaat gcttgatata
     1681 taagtgtttt tacctattag cttaaatgta gattttcctt tatatgaagc gatgcatgaa
     1741 acccttgttt ttattacttt aggaaacaaa tcaaagattt gttgttttta gggtttagta
     1801 tgcaagttgg atgaattaga gggttgtgtg tttaggatag atgttttatt taatagatat
     1861 taagtatttt aagttgaata gatttaatga tttaatgata taaatttgat atatttgtaa
     1921 tcataaaagc ttacgaaaat ataataaaaa tggaggaaat tcaccagttt ctttattgaa
     1981 aggaggaaat aaatgaagaa taaaaaaaga atacttttaa tagccatagc attcttaatc
     2041 cttgcattaa cagcttgtac aaatggtaaa gaagaaaata aaagctctgt gggagaaaca
     2101 gacaaaagtc aacaagaaat aaacaatgag gacaaggttg atagtacaga cgactatgaa
     2161 aataaaccaa atttctttat aggaacaatt gaagaaataa atggggaagt agctcttgtt
     2221 tctattgaga aaggggaaat tcttaaatct ggtagtaagg tgggagtgga cctttctgtt
     2281 gcaaatgata aaacgtttca aattggaaac aaggttaagg ttgggtatga cggcatcata
     2341 a
//
LOCUS       DATGSI010000054         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_51283, whole genome shotgun sequence.
ACCESSION   DATGSI010000054 DATGSI010000000
VERSION     DATGSI010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51283"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VK031_00870"
     CDS             <1..1053
                     /locus_tag="VK031_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52499.1"
                     /translation="FFILGFTMYVTSLFFWPTPATALIGAVLIPVAIESGLPAIYAAM
                     AVNIFGHGMGLAGDLVLQGAPQLAAKSSGVDIELILPISGLLSIIAGVVAIAITFIMM
                     RREMAKEERLAKMDLKRLEEDMPKDPLAKAFGRIVPLILLAVIVVMVVNKINGGAATA
                     LLGGTGIAILLASTVCREKGKSLEEVVKHLREGFIFSIKIFSPVIPIAGFFFLGGSES
                     AMILGENAPKLLFDIGEAVARVLPLNKVSLGFGNMIIGIITGLDGSGFSGIPLVGSLA
                     QALGNPVGVDIPTLASIGQISAVWSGGGTIVAWCFGLAATAGIAGVSPLDLARKNFLP
                     VIIGLIVATTVGIFLM"
     gene            complement(1081..2460)
                     /gene="lpdA"
                     /locus_tag="VK031_00875"
     CDS             complement(1081..2460)
                     /gene="lpdA"
                     /locus_tag="VK031_00875"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HLS52500.1"
                     /translation="MKEYDVLILGGGPGGYVAAIKAAQLGAQVAVIERDELGGICLNW
                     GCIPTKAMLKTAKLYEDILDSQDFGISGIEKDSIRIDWKQLLRRKDRVVKRLVTGIHS
                     LFKKNKIDLYKGTGTVINKNEIRVGNQVLKGKNLILATGAKTYYPKISGLEKAFNAGK
                     LIDSKGALLLEEIPKELIIVGNNNYAVEFVSLFNALGSKVTLIHDGDRILPYMEEEMV
                     KTLERHLKKKKIRLVPKARVKEITSKGILVESKGKEIKYDADKYLFSLGIRPQIEAFK
                     DLGLKLDSKGFVKTNDKMETSIEGVYAIGDINGKYPLAHVASAEAIVAAQNIMEKSSN
                     LDYKLVPQALYSFPELASVGLTEKEAKDKGLDYTTSKFPLIANGKALVEGERVGFLKI
                     ISDNEYGEIIGVHIMSNHATDMISKAVGIMKIEGTVHDLAETIHPHPTTSEIFLEAAL
                     GAVDQPIHM"
     gene            complement(2478..>3275)
                     /locus_tag="VK031_00880"
     CDS             complement(2478..>3275)
                     /locus_tag="VK031_00880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HLS52501.1"
                     /translation="VMKEDIRRAANKKDKAKSDDRPSLQDIEVQVEENVEKVSLTRIR
                     QTIAEQMSNSKFTIPHATAMDEFSIKALVDFRNKYKDKLKNQNTKLTYLPFIIKAALV
                     AMKEFPEFNAQLDLDKDKLILKKYYHIGIATDTERGLMVPVVKNAHHMSLVELAKNIE
                     DVSSRAKKNQIQIEELRGSTFTVTNYGSIGGQFGVPIINYPETAILGVGRAVEKPIVI
                     DGEIGIAPMLTLSLSYDHRVIDGAMAARFLNLIKDLLSEPELLLLKS"
BASE COUNT          839 a          673 c          650 g         1113 t
ORIGIN      
        1 ttttttattc taggttttac catgtatgtg acttctttgt ttttctggcc tacacctgcc
       61 actgccttaa taggagcagt tttaattcca gtggccatag aatcaggctt gccagctatt
      121 tatgcggcca tggctgtaaa catttttgga catggaatgg gcttggcagg tgatttagtt
      181 ttacaggggg caccgcagtt ggccgcaaaa tcatcaggag tagatattga actcatccta
      241 cctataagtg ggcttttgtc tataattgca ggagttgtgg ccatagctat tacctttatc
      301 atgatgagaa gggaaatggc caaggaggaa aggttggcta agatggatct gaaaagatta
      361 gaggaggata tgcccaagga ccccttggca aaagcctttg gaagaattgt accgcttatt
      421 cttttggctg taatagttgt tatggttgta aacaagataa atgggggagc tgccacagcg
      481 cttttgggtg gcacaggcat agctattttg ctagcatcta cagtttgtag agaaaagggg
      541 aaatctttag aggaagttgt aaaacattta cgggaaggct ttatcttttc cataaagatt
      601 ttttcacctg ttatccccat agctggattt ttcttccttg gtggttctga atctgccatg
      661 atactggggg aaaatgctcc aaagctatta tttgatattg gagaagcagt ggctcgggtc
      721 ttgcccttaa ataaagtttc tttaggcttt gggaatatga taataggtat aataacaggc
      781 ttagatggtt caggtttttc agggatcccc ctagttggta gcctggccca ggccttagga
      841 aacccagtag gggtagatat acctaccttg gcctccatag ggcaaatatc ggccgtttgg
      901 tctggcggtg gtaccattgt ggcctggtgc tttggcttgg ccgcaactgc tggaattgct
      961 ggagtttcgc ccctagacct ggcaaggaaa aatttcctgc ctgtaataat tggtctaatt
     1021 gttgcaacga cagtaggcat attcttaatg taaaaaaggc tagataatct agcctttttt
     1081 ctacatatga ataggttggt caactgcacc taaggcagct tctaaaaata tttcagaggt
     1141 agtagggtgg ggatgaatag tttcagccaa atcatggaca gtaccttcaa ttttcataat
     1201 ccctacagcc ttggatatca tatctgttgc atggttggac ataatatgaa ctccaataat
     1261 ctctccgtat tcattgtcgg atataatttt aagaaatccc accctttccc cttcaaccag
     1321 ggcctttcca ttggctatta ggggaaactt acttgtggta taatctagac ctttatcctt
     1381 ggcttccttt tcagtaaggc ctacgctggc caattctggg aaggaataaa gggcctgggg
     1441 aactagctta taatctaaat tactagattt ttccataata ttttgtgctg caacaattgc
     1501 ttctgcagaa gctacatggg ccagaggata ttttccattt atatcgccta tggcatagac
     1561 tccttcaata ctagtctcca tcttatcatt tgtttttacg aaaccttttg aatctagttt
     1621 taaacctaag tctttaaagg cttctatttg tggcctaatt cctaaagaga acaagtactt
     1681 atcggcatca tacttgatct ccttaccctt tgattcaacc aggatgcctt ttgaagttat
     1741 ttcttttacc ctggcctttg gtaccagtct gatctttttc ttttttaagt gtctttctag
     1801 ggtcttaacc atttcttctt ccatataagg caggatccta tctccatcat ggattagagt
     1861 gaccttggag cctagggcat taaagagaga aacaaattct acagcataat tattgttacc
     1921 tactatgatt agctccttag gaatttcctc caaaagaagg gcgcccttac tgtcaattag
     1981 cttacctgca ttaaaggcct tttctagacc tgaaatttta gggtagtagg ttttagctcc
     2041 ggtggccagg attaaatttt tacccttaag cacctggtta cctaccctta tttcattttt
     2101 atttattaca gttccagttc ctttatatag gtctatttta ttttttttaa atagggaatg
     2161 gatacctgtg accagtcttt ttacgaccct atcttttctt ctaagcagct gtttccagtc
     2221 tatacgtata ctatcctttt ctattccgct tatgccaaaa tcctggctat ctaaaatatc
     2281 ttcataaagt ttagctgttt ttaacatggc cttggtaggt atacatcccc agtttagaca
     2341 tatgccacca agttcatccc tttctataac tgcaacttga gcccctagct gggctgcctt
     2401 aatggcagcc acataacctc cagggcctcc gcctaatatt aaaacatcat attctttcat
     2461 attatcacct tagtccttta tgattttaag agcaatagct ctggttctga tagaagatcc
     2521 ttaatcagat ttaaaaagcg ggcagccatg gccccatcta taaccctgtg gtcataggat
     2581 aaggataggg tcagcatagg ggctatgcca atttccccat caattactat tggcttttca
     2641 actgccctgc ctacgcctag gatggcagtt tcggggtagt taattatggg aactccaaac
     2701 tggcctccta tggagccata gttggtaaca gtaaaggtgc tacctctaag ctcttcaatc
     2761 tggatctggt ttttcttggc cctgctgctt acatcctcaa tatttttagc cagctccacc
     2821 aggctcatat gatgggcatt tttaactact ggtaccatga gtcctctttc tgtatctgtg
     2881 gctataccaa tgtgataata tttttttagg attaacttat ctttatctaa atctagctga
     2941 gcattaaatt ctggaaattc cttcattgca accagtgctg ccttgattat aaatggcaga
     3001 taggttagct tagtattttg atttttcaat ttatccttat atttattcct aaagtcaacc
     3061 agggctttta tactaaattc atccatggcc gtagcatggg ggatggtaaa ctttgaattt
     3121 gacatttgtt ctgcaatagt ttgccgaatc ctggttaagg aaaccttctc tacattttcc
     3181 tctacttgaa cttctatatc ttgaagggaa ggcctatcat ctgatttagc cttatctttc
     3241 ttatttgcag cccttcttat atcttccttc ataac
//
LOCUS       DATGSI010000055         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_193697, whole genome shotgun sequence.
ACCESSION   DATGSI010000055 DATGSI010000000
VERSION     DATGSI010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193697"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="VK031_00885"
     CDS             complement(<1..1712)
                     /locus_tag="VK031_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HLS52502.1"
                     /translation="MRLVIAEKPSVAQSIGAVLGANSRKDGYMEGSGYIVTWCVGHLL
                     ELAPAHLYDEKHTRWQYKDLPILPQDWKYIISKGKEKQMKIIGQLMKRAEVKEIIAAT
                     DSGREGELIFRLVYEKLRCKKPIKRLWISSMEESAIAKGFENLRNGTDYELLYQSALC
                     RAKADWIVGINGTRLFSVLYGQTLNVGRVMTPTLAMIVERQVNISEFKAEPFYIVELH
                     CGDFILSSEKIKDKEIAETLCKSCDGQSIAIEKVEEKEKTEKPPKLYDLTTLQREANR
                     QLGFTANQTLEYIQSLYEKKLVTYPRTDSRYLTEDMKDSIPALVNINAGIFLSQDTNL
                     PINIEQVIDNSKVSDHHGIITTMGIENYDIHSLPFGEKEILKIVAIGLISAVGDNHHY
                     AEAIITANCNETIFTVKGKTILEDGFKSVENLYLKNKKKQAKDDKDKVLPAISQGEVF
                     TAKASIKEGKTSPPKHYTDDTLLSAMGNANNALEDMGRKGIGTPATRAGILEKLIKTG
                     FAERKGDKKAKHFIPTHKGISLITILPEIIQSPILTAEWEEKLKQIEQGDLSPDEYLQ
                     EINE"
     gene            complement(1709..1882)
                     /locus_tag="VK031_00890"
     CDS             complement(1709..1882)
                     /locus_tag="VK031_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52503.1"
                     /translation="MAYFTDSVYERMMTQRPRYRQENETPALKKKNVSNGIDNKLKYR
                     KLIIITKEGSEKE"
     gene            complement(1882..2589)
                     /locus_tag="VK031_00895"
     CDS             complement(1882..2589)
                     /locus_tag="VK031_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HLS52504.1"
                     /translation="MKNKSRILITLLTLMLCFTTFSTVAFAGGGEEEIPTIPEVNEPV
                     KSSPTALTPDGNLTLVDDVSGEQAEDKQFVTVISKNGNYFYLVIDRAGDRQNVYFLNL
                     VDEEDLLALIEEDKPKQQEVPQVCNCINLCEEGKIDTNCPLCRNDLSKCTGKPLTPTS
                     TSEPEKGKNKGMGGIVLLLIVGMAGGGIYYFKVLKNKPNTKGNTILDDYDFEDDEDDD
                     YEYETEIDDEDESEVDI"
     gene            complement(2579..2827)
                     /locus_tag="VK031_00900"
     CDS             complement(2579..2827)
                     /locus_tag="VK031_00900"
                     /inference="COORDINATES: protein motif:HMM:NF025556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HLS52505.1"
                     /translation="MSKKKQKVISDIKKTKEKISDLQSKLRQLENQKTELENTEIVEL
                     VRGTKMNTSELAIFLKAYREKGDASFIMEKEEERNHEE"
BASE COUNT          793 a          617 c          440 g         1082 t
ORIGIN      
        1 atttcattga tttcctgtaa atattcatct ggtgaaagat caccctgttc aatctgtttt
       61 agcttttcct cccactcagc agtaagtata ggtgactgga tgatttctgg caaaatggta
      121 ataagggaaa tgcctttatg tgtaggaata aaatgtttag cttttttatc tcctttacgc
      181 tctgcaaatc ctgtcttaat aagcttttct aaaattcctg cacgggtggc aggagttcca
      241 ataccttttc tccccatatc ctccagtgca ttattggcat ttcccatagc agaaagcaag
      301 gtatcatctg tatagtgttt tggcgggcta gttttacctt ctttaatcga tgcttttgca
      361 gtaaacactt ctccttggga aattgctggt aagactttat ccttatcatc tttagcttgt
      421 ttctttttat ttttaagata taagttttca actgatttaa atccatcttc aaggattgtc
      481 ttacccttta cagtaaatat agtttcatta cagttagctg ttataatggc ctcagcataa
      541 tggtgattat ctcccacggc actaattaag cctatggcaa ctatctttaa tatttccttt
      601 tctccaaagg gcagtgaatg aatatcatag ttttcaatac ccatagtggt aataattccg
      661 tgatgatcac ttaccttact gttgtcaatt acctgttcta tatttatggg taggttagta
      721 tcttgggata aaaatatccc tgcattgata tttaccaagg cagggatact atctttcata
      781 tcttctgtta aataacggct atcagtacgg ggataggtaa caagtttttt ctcataaagg
      841 ctttgtatat attcaagagt ctgattagca gtaaagccta attgcctatt tgcttccctt
      901 tgcaaagtag ttaaatcata tagctttggt ggtttttctg tcttttcttt ttcctctacc
      961 ttttctatgg caatactttg tccatcgcag gatttacaaa gtgtttcagc tatttcctta
     1021 tcttttatct tctcacttga aagaatgaaa tcaccacaat gtagttctac aatataaaat
     1081 ggctctgctt taaattctga aatatttact tgtctttcta ctatcatggc aagggttggg
     1141 gtcattaccc tgcccacatt taaggtctgt ccgtaaagga cagaaaaaag acgggttcca
     1201 ttaatcccta caatccaatc tgccttggcc ctgcaaagtg ccgattgata taaaagctca
     1261 tagtctgtcc catttcgtag attctcaaat cccttggcaa tggcactttc ctccattgaa
     1321 gaaatccata atcttttaat aggtttcttg catcttaatt tctcatatac caatctaaaa
     1381 attaattctc cttcacgccc tgaatccgta gctgctataa tttctttaac ttctgctcgt
     1441 ttcattaact gaccaataat tttcatttgc ttttccttac cctttgagat gatatatttc
     1501 caatcttgcg gtaaaatggg caaatcctta tactgccacc ttgtatgttt ctcatcatat
     1561 aaatgggctg gggcaagttc caacaaatgc cccacacacc atgttacaat atagccacta
     1621 ccctccatat atccatcttt cctactatta gcacctaaaa cagctcctat actttgggct
     1681 acagatggct tttctgcaat aaccagtctc attctttttc acttccttcc tttgtaataa
     1741 taatcagctt acgatattta agcttattat ctataccatt acttacattc ttctttttta
     1801 aggcaggagt ttcattctcc tgcctgtatc ttggtctttg ggtcatcatc cgttcataaa
     1861 cactatccgt aaaataagcc actaaatatc aacctcactt tcatcttcat catcaatttc
     1921 tgtttcatat tcataatcat cgtcttcatc atcctcaaag tcataatcat caaggatggt
     1981 atttccttta gtatttggct tgttttttaa cactttaaaa taataaattc ctccacctgc
     2041 cataccgact attagtaaga gtacgatacc acccataccc ttattttttc ccttttcagg
     2101 ttcagaggta gatgtaggag ttaatggctt tcctgtgcat ttagaaagat catttctaca
     2161 aaggggacaa ttggtgtcta ttttaccttc ctcacaaaga ttaatacagt tacatacctg
     2221 tggtacttct tgctgtttgg gtttatcttc ctcaattaag gcaagtaaat cttcttcatc
     2281 tacaagatta aggaaatata cattttgcct atcccctgca cgatctataa ctagatagaa
     2341 atagttaccg ttctttgaaa taacagtaac gaattgttta tcttctgcct gttctcctga
     2401 aacatcgtct actagggtta aattaccatc aggtgtaagg gcagttggac tagactttac
     2461 tggctcatta acttctggaa tagttggtat ttcttcctca ccacctcctg caaaggctac
     2521 tgttgaaaat gtagtaaagc aaagcataag ggtaagtaag gtaatcagta tgcgactttt
     2581 attcttcatg gtttctttcc tcctcttttt ccataataaa agacgcatca cctttttcac
     2641 gatacgcctt taaaaatata gcaagttcac tggtattcat ttttgtacca cggacaagtt
     2701 ccacaatttc cgtgttttca agttctgttt tctgattttc aagctgccta agtttgctct
     2761 gtaaatcaga tattttttcc tttgttttct taatatctga aataactttt tgttttttct
     2821 tactcatgtc ttcctcctta atttaatctt ccataagcat agaaatgctc tgtccagtag
     2881 gggctattaa ctgaagcata ctggatagga ttgccacaat ggatcatcat ac
//
LOCUS       DATGSI010000056         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_5487, whole genome shotgun sequence.
ACCESSION   DATGSI010000056 DATGSI010000000
VERSION     DATGSI010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5487"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VK031_00905"
     CDS             complement(<1..503)
                     /locus_tag="VK031_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLS52506.1"
                     /translation="MKQSILVVDDESSITTLIAYNLEVAGYKTEVAHDGETALHKIKS
                     RHYDLVVLDIMLPKLSGIEVCQILRAEDYKLPILMVTAKTDVEDIIEGLNTGADDYIT
                     KPFSPQELVARVHAILRRAQEDVREDEFRSGPIQVFPNRYEAYFEGELLTLTKKEFEL
                     LSYLVKN"
     gene            complement(859..1659)
                     /locus_tag="VK031_00910"
     CDS             complement(859..1659)
                     /locus_tag="VK031_00910"
                     /inference="COORDINATES: protein motif:HMM:NF017808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLS52507.1"
                     /translation="MKKRISWLVGAGLLLRGLYRKKTSSSKGKKVTVFVHGYLGNYQS
                     FAPLLSRFEGQYKWGEKVGIYYVFPNNQLKYVPVKTSSSGQDLIQVVFPDNKGSFHDY
                     ANWLSLLMRDLKQKDPRASFNLVGHSMGGIACIKYLQDFSNRKSFPRVEKVVTLGSPF
                     AGISDDEYFKQHGLNRATLDLRSGSEAFKSLRQNREKIPYHTDILNIGSLGDQVASPA
                     SVQALNQLVHVKNIENYIIQRQGVSHSGLHEDEEVDQLISAFLGLGDL"
     gene            complement(1756..>2216)
                     /locus_tag="VK031_00915"
     CDS             complement(1756..>2216)
                     /locus_tag="VK031_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLS52508.1"
                     /translation="VIASSSHEVGTAPMPVMDGVEPKGAVVGGASLWITNQVSEEEQA
                     AAWEFIKFTTKADVQAEWAAGTGYFPITPAAYEEQVLKDIYEEYPQYTTAVKQLENTV
                     NTASTAGALFSIMPEARKIIEMGLGEVYEGMDPQEALDDAAKKITEALQK"
BASE COUNT          633 a          528 c          384 g          671 t
ORIGIN      
        1 ttatttttaa ccaggtaaga aagaagttca aactcctttt tcgtcaatgt gagtagctca
       61 ccctcaaaat aggcctcata gcgatttgga aaaacctgaa tgggtcccga ccggaactca
      121 tcttcccgta catcctcctg ggcccggcgt aaaatagcat ggactcgagc tactaattct
      181 tggggactaa agggctttgt aatatagtca tccgcccccg tatttaaccc ttcaataata
      241 tcttcaacat ccgtttttgc cgttaccata aggattggta gtttataatc ttcagcccga
      301 agaatctggc aaacctcgat cccacttaat ttcggcagca taatatctaa aacgactaaa
      361 tcataatgtc tgcttttaat tttatgtaag gcagtctctc catcgtgggc aacctcagtt
      421 ttataaccag ccacctctag attataagca attaatgtcg taatcgacga ctcatcatcc
      481 acaactaaaa tcgattgttt caaatagaaa actccttcgc taaattgata tctctttctc
      541 ttattgtata tccttagaaa ggaaaaataa atcctatcca tgtaaaaaaa ttgtaaattt
      601 ttcgctgtgg aagatgtcta cacccattaa ctgtaaaata acacgagtaa agcctgggct
      661 agtaggtagc tatcatcgct actgttcctt agatagtata aaactagttg atctcttacc
      721 ctagccccta gatgattttg cttagactca ctccttatct agcatctgcc aaatccaata
      781 aggaggaaag ttttttcaag ggcttcttcc ggggatgggt tgctcgataa gtacgaacca
      841 attagacaat gagttgccct agagatctcc tagccctaaa aaggcagata ttaactgatc
      901 gacttcctca tcttcatgca ggccactatg gcttacccct tgtcgttgaa taatatagtt
      961 ttctatattt ttcacatgga ccagctgatt aagggcttga acactagcag gactagctac
     1021 ttgatctcca aggctgccaa tatttaagat atctgtatga tagggaatct tctccctatt
     1081 ttgccttaag ctcttaaaag cctctgatcc gcttcgtaaa tcaagggttg ccctatttaa
     1141 tccatgttgt ttgaaatatt catcatcact gatgccggca aaaggactac ctaaagtcac
     1201 aaccttctct accctgggga aagacttcct atttgaaaag tcctgcaaat acttgataca
     1261 ggcaattcct cccatcgaat gaccgactaa gttaaagctt gccctaggat ccttctgctt
     1321 taaatccctc atcagcaaac ttagccagtt ggcataatca tgaaaactcc ccttattgtc
     1381 tggaaacact acttgaatca ggtcctggcc agagctggaa gttttaaccg gtacatattt
     1441 aagctgatta ttgggaaaaa catagtatat ccctactttc tctccccatt tatactgccc
     1501 ttcaaagcgg cttaataaag gtgcgaagga ctgataattc cctaaatatc catgaacaaa
     1561 gaccgtaacc ttctttccct tagaagagga agttttcttt cgataaagcc cccttagaag
     1621 caagcctgca ccgactagcc agcttattct ttttttcaca agtactaacc tcctttccta
     1681 gatcataacg agaaaaataa atagattcaa agattagccc atcccaatgg agagtaatct
     1741 ttgaatccat ctagcctatt tctgtaaggc ttcggttatt ttcttcgcag cgtcatctaa
     1801 ggcttcttgc ggatccattc cctcatatac ttcacctaag cccatttcta taatcttacg
     1861 ggcctcaggc ataattgaga ataaggctcc tgccgttgaa gcggtattca ctgtattttc
     1921 taattgctta acagcagttg tatattgagg atactcctca taaatatcct ttaaaacttg
     1981 ctcctcatag gcagctggag taattgggaa ataacctgtt cctgctgccc attcagcctg
     2041 tacatccgct ttggttgtaa acttaataaa ttcccaggct gccgcttgct cctcttcaga
     2101 tacttgattg gtaatccaga gagaggctcc acctactaca gcaccctttg gttctactcc
     2161 atccataact ggcatagggg ctgtcccaac ctcgtgggag gatgaggcga tgactt
//
LOCUS       DATGSI010000057         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_427985, whole genome shotgun sequence.
ACCESSION   DATGSI010000057 DATGSI010000000
VERSION     DATGSI010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427985"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..374
                     /locus_tag="VK031_00920"
     CDS             96..374
                     /locus_tag="VK031_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52509.1"
                     /translation="MKEVLAIVAFKIVFDFILGRIDREIPEIFAYIRNKTLKLREKLT
                     LRILSLLIFITIFALIVTYLELDYIYGGAVMGLGLSLFDKAFRDKRGG"
     gene            412..1518
                     /locus_tag="VK031_00925"
     CDS             412..1518
                     /locus_tag="VK031_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLS52510.1"
                     /translation="MNVKEISKNISVMIKPSSSKCQMACQYCFYRDLAESRKVKDFGY
                     MNDGTRDNIIEKTLRFAAGGDVTFAFQGGEPTLIGLDFYKEFKAKVDLLNQNNCGINY
                     SIQTNGMKIDKDWIGWLKENKVLVGISLDGTEKIHNIFRQVRKRQGTFNQVYGTIKSL
                     QAEQVDFNILTVVTDRLACDIEEVFKFYIREGFQYLQFIPLIYSKDDKILTGKNFGRF
                     LVNLYDLWVKELKKGNQVSIRFFDNLSALIKGYDNLACEMRGHCSMQNIIEADGSIYS
                     CDFYATEEYKIGNINQLGFYQIYKNERVINFIKSSFERHRSCKSCNFFIYCRGGCRRH
                     LLGTGKNLYCGGYKYFFENRLEDFKSLIRKGKIK"
     gene            1644..2036
                     /locus_tag="VK031_00930"
     CDS             1644..2036
                     /locus_tag="VK031_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HLS52511.1"
                     /translation="MIGVLLVSHGEFCKELLNSAEMIIGKQKNVVSLSLKEGESIENL
                     FAKVDREIERLDNGKGVIILTDLFGGSPSNVAALKLGEKNIESLTGVNLPMLIEVLNS
                     RETSSLKDLAKLGHKSAVDGVQNLRDHK"
BASE COUNT          744 a          221 c          462 g          620 t
ORIGIN      
        1 gattaataac tagatcatgg atgtttttat tattcgtcga catcctctta tattatgtta
       61 taattaactt aataaaacaa cagggggaag ctgaaatgaa agaggtattg gctatagtgg
      121 catttaaaat tgtatttgac tttattttag gcaggataga tagggagata ccggagatat
      181 ttgcatatat ccgtaataaa accttaaagt taagggaaaa attaacttta agaatccttt
      241 ccctgctaat attcataacc atatttgcct tgatagtaac ctatttagaa ttagactaca
      301 tatatggtgg ggcagtaatg ggattgggtc tcagcctatt tgacaaagcc tttagggata
      361 aaaggggagg ctgatattag ctgttgctcc agttggaggt aagtatattt ggtgaatgta
      421 aaggagataa gtaaaaatat ttctgtgatg ataaagccat cctcaagtaa atgccagatg
      481 gcctgccagt attgttttta tagggatttg gcagaaagta gaaaagtcaa agattttggt
      541 tatatgaatg atgggaccag ggataatata atagagaaaa cattaagatt tgcagccggg
      601 ggagatgtaa cctttgcttt tcaaggtggg gaacctacac ttataggttt agatttttat
      661 aaggaattta aggctaaagt ggatttacta aaccaaaata attgtggcat aaattatagc
      721 attcagacca atggaatgaa aatagataag gactggatag gctggttaaa ggaaaataaa
      781 gttttggtag gcatatctct agatggaact gaaaagatcc ataatatttt tagacaagta
      841 agaaagaggc agggtacctt caatcaagta tatgggacaa ttaaaagttt acaggcagag
      901 caggtggatt ttaacatatt aactgttgta acagatagac tagcatgtga tatagaggaa
      961 gtatttaaat tctacataag ggaagggttt caatatttac aatttattcc cctaatctac
     1021 tcaaaagatg ataaaattct aacagggaaa aattttggaa ggtttctggt aaatttatat
     1081 gacttgtggg ttaaggaact aaaaaaggga aatcaggtca gcataaggtt ttttgataat
     1141 ttatcagcat taataaaagg ttatgacaat ttagcctgtg agatgagggg ccattgtagt
     1201 atgcaaaata ttattgaagc agatggcagc atatattcat gtgatttcta tgctacagaa
     1261 gaatataaga tcgggaatat taaccaacta ggtttttatc agatatataa gaatgaaagg
     1321 gttataaatt ttattaaaag ttcatttgaa aggcataggt cctgtaaaag ttgcaacttt
     1381 tttatatatt gcaggggtgg atgcagaaga catttacttg gtacaggaaa gaatttatat
     1441 tgcggaggat ataagtattt ttttgaaaat aggttggaag attttaagag cttaataaga
     1501 aaaggaaaaa ttaagtaaaa tctggtcaaa agaaagctaa atagctttct tttttgtatt
     1561 atatacatat ttttcactct aaggttacat cataagtaag tagaatagct ggttttatta
     1621 gggaaaaaga aggaggggag gatatgatag gggttttgct tgtaagtcat ggagaatttt
     1681 gtaaggaact tttaaatagt gcagaaatga taataggaaa gcaaaaaaat gtagtctcct
     1741 taagcctaaa ggaaggggaa agtatagaga atctatttgc aaaggtagat agagagattg
     1801 agagattaga taatggaaag ggagttatta tactgacaga cctgtttggg ggaagccctt
     1861 ccaatgtggc agctttaaag ttgggagaaa aaaacataga aagcttaacg ggagttaatc
     1921 ttccaatgtt gattgaagtt ttaaattcaa gggagaccag tagcttaaaa gatttggcta
     1981 aactgggcca taaatcggca gtagatgggg ttcaaaacct acgggatcac aaataggtag
     2041 attaaag
//
LOCUS       DATGSI010000058         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_194019, whole genome shotgun sequence.
ACCESSION   DATGSI010000058 DATGSI010000000
VERSION     DATGSI010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194019"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..569
                     /locus_tag="VK031_00935"
     CDS             144..569
                     /locus_tag="VK031_00935"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HLS52512.1"
                     /translation="MKRKDTIEGVILTSLSAFLIKESFKLHNNQEWALSPALFPLLIT
                     VFLFLFSIFLIIKGIKNEEAKYGDKIDWRLIFYVLVWSFIYAFLLPIVHFIPATILYL
                     SIFLLVLGEKKWSHIIFISILTPVLVYYLFNNLFNVFLP"
     gene            587..2071
                     /locus_tag="VK031_00940"
     CDS             587..2071
                     /locus_tag="VK031_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HLS52513.1"
                     /translation="MLANIISTIDIHFIFLVLLGSVGGLFVGALPGLSVTMATALLVS
                     ITFSWEVNHAMALIMGVYTVGVYAGAVSAILVNIPGAPSSVATTLDGYPMAKKGQALL
                     ALRTAAYYSFLGTIIGLIVLAISARPITRLALAFTPLDYFLLALFGLTTVGSLTAGNF
                     TKGMISALLGVLLSTIGMDPITGIGRFTFGSINLQRGIPIIPALIGLFGFSETLFQLG
                     QKYKEHRLINIEEGKIKTREILSHWYLGIRSSIIGVFIGALPGTGGPIAALLAYEHGK
                     NSTKNPKIPFGEGAIEGVVASESANNACIGGALIPMLTLAIPGDAVTAVILSAFYIHG
                     LRPGPLLFSETPEMFAVVLAGGFIGSLMILILGLTVGPMLSKIVNIKKTYLFPVIGVL
                     CVIGSYSASTNIFDIYLMLIFGIIGLVMKVRGYSPAPMVLGLVLGDIMDANFRRAVSL
                     FSASDNLLKDILLKPITLVLLAAVALSIMSNFVFKGKKFTRMSK"
BASE COUNT          721 a          400 c          475 g          815 t
ORIGIN      
        1 accatatgtc agtagataag attaactctt attgggacag atatacagcc attacatcct
       61 ggcttttata tgatgctgga tcagtaccgc atagtccaga ggagtttgga atagaaaggt
      121 atggcgacta actggtggta aatatgaaaa gaaaagatac aatcgaggga gtaattctta
      181 caagtttaag tgcgttttta ataaaagaat cttttaagct tcacaacaac caggaatggg
      241 ccctatcccc agccttattt cctttattaa ttactgtttt cttgttctta ttttccatat
      301 ttttgattat aaaaggaatt aaaaatgagg aagcaaagta tggtgacaaa atcgactgga
      361 gattaatctt ttatgtttta gtatggtcct ttatttatgc cttcttatta cctatagtcc
      421 actttatacc agccaccata ctatatttat ctatcttttt actagtcctg ggcgagaaaa
      481 aatggagcca tataattttt atatcaatac ttacacctgt attagtttat tatttattta
      541 acaatttatt taatgtgttt ttaccttaga agagaggccg atttacatgt tagcaaatat
      601 tatttctact atagatatac attttatatt tttggttctt ttaggttcgg tgggaggatt
      661 gtttgtaggg gccttaccag gcttatctgt aaccatggca acagccctcc tggtatcaat
      721 caccttcagc tgggaagtta atcatgccat ggctttaatt atgggagtct atactgtcgg
      781 tgtatatgct ggtgcagttt ctgccatttt agtaaatatt cctggcgcac catcctctgt
      841 agccaccacc ctggatggct atccaatggc taagaaaggt caggctttgt tagccttaag
      901 aactgcagcc tactattctt ttttaggtac aataattgga ctaatagttc tagcaatttc
      961 agcaaggcct ataacaagac tggcattggc ctttacacct ttagattatt ttcttttagc
     1021 cttgtttgga ctgactactg tcggttcctt aacagctggg aattttacta aaggaatgat
     1081 aagtgccctg ttgggggtat tattaagtac cataggcatg gatcctatta caggcatagg
     1141 tagatttacc tttggcagta taaatttaca aaggggtata cctattattc cagccctaat
     1201 aggtctgttt ggattttctg agactctctt ccaattaggg cagaaatata aggaacatag
     1261 gcttataaat attgaagaag gaaagattaa aacaagagaa attttgtccc actggtacct
     1321 aggtatcagg tcttccatta taggggtttt cataggggcc ttgccaggta caggtggccc
     1381 tatagcagcc cttttagcct atgaacatgg taaaaattct accaaaaacc ctaagattcc
     1441 ctttggagag ggggctattg agggtgttgt agctagtgaa tcagctaata atgcctgtat
     1501 agggggagcc ttaataccta tgttaacatt ggccataccg ggagatgcag taacagctgt
     1561 tattttatct gccttttata tccatggtct aaggcctggt cccttattat ttagtgaaac
     1621 tccagaaatg tttgctgtag ttctagcagg tggctttata ggtagtctta tgatattaat
     1681 tctaggtcta acagtaggac ctatgctatc taagatagta aatataaaga aaacctatct
     1741 ctttcctgta attggggtac tttgtgtaat tggatcctat tctgcaagca caaatatatt
     1801 cgatatatat ttaatgttaa tttttggaat cataggactg gtaatgaagg tgaggggata
     1861 ttctccagcc ccaatggtat tgggcctggt tttgggggac ataatggatg caaactttag
     1921 aagggccgtt tctttattta gtgcctctga taatctatta aaagatatat tattaaaacc
     1981 tataacactg gtacttcttg cggctgttgc attgtcaata atgtctaatt ttgttttcaa
     2041 aggtaaaaag tttaccagaa tgagtaaata aacttatttt aaaggcagat tttaggaatt
     2101 tgacaaaaaa tctaacagcc actgataaat cttttgttgc ttaaaatcta atagatctgg
     2161 ctgatcatgg ctttaagcga gattatttaa gtaaatttat agcagttcag tttaaacagc
     2221 atattataca atttcataac ttcacatcct tatgacaaac ttttcccaaa ttaagttaat
     2281 gagccccttt catgggtata ggctatatga aagataaaaa acggaggggt ttatatggat
     2341 tataggattg aacatgacac catgggggaa gttagggtac ctatagaaaa atactggggt
     2401 gctcaaaccc a
//
LOCUS       DATGSI010000059         1378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_51797, whole genome shotgun sequence.
ACCESSION   DATGSI010000059 DATGSI010000000
VERSION     DATGSI010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51797"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..795
                     /locus_tag="VK031_00945"
     CDS             88..795
                     /locus_tag="VK031_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HLS52514.1"
                     /translation="MTRDKKTIVKDALKLVLPIVLGYIPVAMAFGVLARNIEISLFHT
                     SLFSIMVYAGASQFMALDLLQSGIGGMSIILTTFLLNLRHMVMSASISTKFTNIKRSS
                     FPIVAFGLTDETFSILYFTKNKLSLTFVLTVNLMAYLSWVLATIVGYLLGEALPASLQ
                     ASLGIGLYGMFAALLIPQLKGKRNNLLVSFLAVIIYLLIDYMKIFTTGWDIVLGIVLS
                     SLIGVFLFRGEGEEVND"
     gene            1249..>1378
                     /locus_tag="VK031_00950"
     CDS             1249..>1378
                     /locus_tag="VK031_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004818592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HLS52515.1"
                     /translation="MKVIVFLADGFEEVEALTVVDYLRRMDIEVDTVSIGEEREVKG"
BASE COUNT          432 a          185 c          289 g          472 t
ORIGIN      
        1 gtatttaaaa gaaacaagga aaaattggag gataatggag ctaaagatgg acctggcagg
       61 aagtcaaatt atgaaaggat aatatagatg acaagagaca agaaaaccat tgtaaaagat
      121 gctttaaagt tagtgctacc tattgtacta ggttatatac cagttgctat ggcctttggg
      181 gtactggcac gaaatataga aatatcttta ttccatacca gcctattttc cataatggta
      241 tatgcaggag ccagtcaatt tatggctctt gatttattgc aatcaggtat agggggtatg
      301 agtattatac tgactacctt tttattaaat cttagacaca tggttatgag tgcatcaatc
      361 tcaaccaaat ttactaatat taaaagatca agtttcccca tagttgcctt tggacttaca
      421 gatgagacct tttctatttt atactttaca aagaataaac ttagtcttac ttttgtgttg
      481 actgttaatt taatggctta tttatcttgg gtgctagcta ctattgtagg atatttactg
      541 ggagaggcct tgcctgcatc ccttcaggct agcctaggca taggcttata tggcatgttt
      601 gcagcccttt taatccccca gctaaaaggg aaaagaaata atttattagt atccttcctg
      661 gctgttatta tatatttatt aattgattat atgaaaatct ttacaacagg ctgggatatt
      721 gtcctaggga tagttttatc atcccttata ggagtatttc tttttagggg agagggggaa
      781 gaagttaatg attaggtgga taataatagg gatggctcta gttacttata tacctaggct
      841 attacccttg tattttttag ccaataagca attaaatccc aaattagagg agtttttgaa
      901 ttatatccct tacacttctc ttagtatact tttggcccgt ggagttatga cctcgtctaa
      961 tgaactaata tttcctacaa ctgtagggat tacactggca gggttggtgt cctattatag
     1021 gccaaatttg gttgtgtcag ttctaatttc tataattggt tcttttataa ttataaatat
     1081 ataaaaactg atttaaatgg aagtgatggc taaagcaatc ttttttataa ataagttata
     1141 cctatatata aggtttgtct tagggcaatg acaatgataa aagtttttag gccttaaata
     1201 ggctaagtgg taaatttagg gtataatata gacaaggagg gagtttttgt gaaggtaata
     1261 gtatttttgg ctgatggttt tgaagaggtg gaagccttaa cggtggtgga ttatttaagg
     1321 aggatggata tagaagtaga tactgtatcc ataggagaag aaagggaagt taaagggg
//
LOCUS       DATGSI010000060         1707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_51798, whole genome shotgun sequence.
ACCESSION   DATGSI010000060 DATGSI010000000
VERSION     DATGSI010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51798"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VK031_00955"
     CDS             complement(<1..903)
                     /locus_tag="VK031_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52516.1"
                     /translation="MAKLKDQNIKSDKIIGERNNVTKINNTFLSERYLSKITVGSTET
                     LEPTEPAQVIETGDVSEKVLNFKIPRGLKGDSGKDGKDGQDGAEGEKGHDLEYRYKVG
                     TSTTVAPEIDRFKRVPEGWSDEVPNISQGMYLWEVKAMINSKNDLVDKWSRPYRSTGE
                     KGDKGNDGLDGEDGMDGVDGEDGKSGATLNPRGDWSNSEQYSGTELVVEFVKYNNKGY
                     LTRIDAGDIPIGTVPTDTDYWNEITDNPDSIFTDFLVAVSAAIENLTVGTIQTGADGE
                     RTTIGYKPEDSSDPTSDYSKHSIRG"
     gene            complement(918..1142)
                     /locus_tag="VK031_00960"
     CDS             complement(918..1142)
                     /locus_tag="VK031_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52517.1"
                     /translation="MVTDMARKNLSGNRGKFDDIDEMIVYLKILGDFEISDFIENDVP
                     NLLTVNDIYAIHNRAMYLLNRNNVKKTYVK"
     gene            complement(1202..>1707)
                     /locus_tag="VK031_00965"
     CDS             complement(1202..>1707)
                     /locus_tag="VK031_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52518.1"
                     /translation="ENESYESFRGGVLDINYYVITGSIELEGEKGDEYLIGEDLHLIY
                     ENFDSIVAGGKIYKINKTIPTNAGTVLYLTKPLEEDVSEVEVAYRGNLKVKNDYDTRK
                     ILAKTARVLTYRDATPSIWDRKEAIMKLIHNWEASKILWELEEYDEVDLILSENVEIG
                     YYLGLIC"
BASE COUNT          406 a          396 c          245 g          660 t
ORIGIN      
        1 ccctcttata gaatgttttg agtagtccga agtcgggtct gatgaatctt ctggtttata
       61 gcctatagtt gttctctcac catctgctcc agtctgaata gttcctacag taaggttttc
      121 tatagccgca gaaacagcta ccaagaagtc tgtaaatatg gaatctgggt tgtcggttat
      181 ctcattccag tagtctgtat cagttggaac agtacctatt ggaatatctc ctgcatctat
      241 tcttgtaagg tatcctttgt tattatactt tacaaactct actaccagct ctgttccact
      301 atattgctct gagttgctcc agtctcctct tgggttaagt gtagcccccg atttaccatc
      361 ttctccgtca accccgtcca ttccgtcctc accatctaac ccatcgttac ctttatcacc
      421 cttctctcct gtacttctat aaggtctcga ccacttatct actaaatcat ttttagagtt
      481 tatcatggct tttacttccc ataggtacat accctgagat atgttgggaa cttcgtctga
      541 ccatccttcg ggaactctct taaatctgtc tatttctggg gctactgttg tacttgtacc
      601 tactttgtac ctatattcta agtcatgacc tttctctcct tcagcaccgt cctgaccgtc
      661 cttgccatct ttaccactgt ctccttttag acctctcggt attttaaagt tcaatacctt
      721 ttcagatacg tctcctgttt ctattacttg ggcaggttcc gtaggttcta aagtctcagt
      781 acttcccaca gtaatttttg ataaatatct ttcactaagg aatgtgttgt ttattttggt
      841 tacattattc ctttccccta ttatcttatc tgattttata ttttggtctt ttagtttagc
      901 cattataatg gtttgtttta tttaacgtat gttttcttta cattgttcct gtttagtaag
      961 tacatagctc tgttgtgtat agcgtatata tcatttacag ttaataagtt gggtacatcg
     1021 ttctctataa aatctgatat ttcaaaatca cctaatatct ttaggtacac tatcatctca
     1081 tctatgtcgt cgaacttacc tctgttacct gataggttct ttctagccat gtcagttacc
     1141 atatcaaggt atccctccct aatatttcgg tacatgttgt ttaattcttt ctcagttatt
     1201 gttagcatat caaacctaaa tagtaaccta tctccacatt ttcacttaat atcaaatcta
     1261 cttcgtcata ctcttccagc tcccataata tttttgaagc ttcccagtta tgtataagtt
     1321 tcattatagc ttctttccta tcccatatag aaggagtggc atctctgtaa gtaagtaccc
     1381 ttgcagtctt ggccagtatt ttacgggtgt catagtcatt cttaacttta aggttacccc
     1441 tgtaagccac ctctacctca cttacatctt cttccaaagg tttagtaagg taaagaactg
     1501 ttcctgcatt ggtaggtatt gttttattta tcttgtaaat cttacctcct gcaactatag
     1561 aatcgaagtt ctcgtatatc aggtgtaaat cctctcctat aaggtattca tctccttttt
     1621 ctccctctaa ctctattgaa ccagttatta catagtagtt tatatctaac accccacctc
     1681 taaaagattc atagctctca ttttcat
//
LOCUS       DATGSI010000061         1896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_194167, whole genome shotgun sequence.
ACCESSION   DATGSI010000061 DATGSI010000000
VERSION     DATGSI010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194167"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VK031_00970"
     CDS             <1..914
                     /locus_tag="VK031_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS52519.1"
                     /translation="TLELGVVYAFIDYVKQFFNPINNLAAEYNTIQSGIVSADRIFEL
                     LDTEDMLEDLESGEKVEEFKGKIEFKNVWFAYEEDEWILKDVSFTINPGETVAFVGST
                     GAGKTTIINLINRFYDIQKGEILLDGKNIENYRMGDLRRNVAVVLQDVFLFSESVKDN
                     IRLNSNISDERVEEAIELSGFGEFADELPGGLDEEVMEKGSKFSAGQRQLLSFARAIA
                     HDPSILVLDEATSNIDTNTETIIQESIKNISKDRTTLIIAHRLSTIRDADKIILLDKG
                     EIVEIGNHEELMEKDSYYRGLHEAQYA"
     gene            968..>1896
                     /locus_tag="VK031_00975"
     CDS             968..>1896
                     /locus_tag="VK031_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLS52520.1"
                     /translation="MLIVKNCKLMDMVDVDGEIRDIYINKEGKFELVEKNIDPSSYPD
                     AKVIDAEERLVTPGFVEPHCHMGVYETGIREGMDGNETTNPAVPGLRAIDAIDPMDAA
                     YDVAIRHGVTTVVTGPGSSNIIGGTYAAMKTAGNILDKALIKDELMMKTALGENPKMN
                     YGRRGKAPKTRMMSAAILREKLFEAKEYYEEYKSLKEQGEEEKLDYDHGLHSLMRVFD
                     GMKLKIHAHQADDILTGIRIAEEFGLRYTIDHCSEGYLIADVLKEKNVQCILGPTVGG
                     KGKYESRNKSLESAAILEAEGVDFAIMTDHPVIP"
BASE COUNT          664 a          249 c          461 g          522 t
ORIGIN      
        1 agaccttaga actaggtgta gtatacgcct ttatagatta tgtaaagcaa tttttcaatc
       61 caataaataa tttggctgct gagtataata ctatccagtc tggaatagtt tctgcagata
      121 gaatttttga attactagat acggaagata tgctggaaga tttggagtca ggtgagaagg
      181 tagaagagtt taagggaaaa atagagttta aaaatgtttg gtttgcctat gaagaggatg
      241 aatggatatt aaaggatgta agttttacaa ttaatcctgg tgaaactgtg gcctttgtgg
      301 gctcaactgg ggcaggtaaa accactatta ttaatcttat caataggttt tatgatatac
      361 agaaggggga gattttatta gacggaaaaa atattgaaaa ctataggatg ggtgatttaa
      421 ggagaaatgt ggctgttgtt ttgcaagatg tgtttctatt ctctgaatct gtaaaggaca
      481 atataaggct taattccaat atatctgatg aaagggtaga agaggccata gaattatcag
      541 gttttggaga atttgctgat gagctgcccg gtggcctaga tgaggaggtt atggaaaagg
      601 gaagtaaatt ttctgcaggg caaaggcaac tcctatcttt tgccagggcc atagcccatg
      661 atccttctat acttgtgcta gatgaggcca cgtcaaatat agatactaat acagaaacca
      721 taattcagga atcaataaag aatatatcaa aggacagaac cacattaata atagcccaca
      781 ggttgtctac cataagggat gcagataaga ttatcctcct agataaagga gaaatagtag
      841 aaataggtaa ccatgaggaa cttatggaga aggatagtta ctatagaggc ttacatgagg
      901 cccaatatgc ttaagtagtt tttaggagaa aaataggaca gttttttata ttttgagggg
      961 gaaaattatg ttaatagtta aaaactgtaa gctgatggat atggtggatg tggatggtga
     1021 gattagggat atctatataa acaaggaggg gaaatttgaa cttgtagaaa aaaatataga
     1081 tcctagttct tatcccgatg ccaaggtgat tgatgcagag gaaagattgg taacaccagg
     1141 ctttgttgaa cctcactgtc acatgggagt gtatgaaaca ggaattaggg aaggtatgga
     1201 tggaaatgaa actactaacc cagctgtacc aggacttagg gccatagatg caatagatcc
     1261 tatggatgca gcctatgatg tggccataag acatggggtg accactgtag ttacaggacc
     1321 aggcagttcc aatataattg gcggaaccta tgctgctatg aaaactgcag gcaatatact
     1381 ggataaggcc ttaatcaaag atgaattaat gatgaaaacg gccttaggtg aaaaccctaa
     1441 aatgaactac ggtagaaggg gcaaggcacc taagaccagg atgatgagtg ctgctatttt
     1501 gagagaaaaa ctatttgagg ccaaagaata ttatgaagag tataaaagtc ttaaggagca
     1561 gggagaagaa gagaaattag actatgatca tggacttcat agtttaatga gggtatttga
     1621 tggtatgaaa ttaaagattc atgctcatca ggcagatgat attttaacag gaattaggat
     1681 tgctgaggaa ttcggtttaa gatatacaat tgaccactgt agtgaaggat atttaatagc
     1741 ggatgtattg aaggaaaaaa atgtccaatg cattttagga cctactgtag gtggaaaagg
     1801 aaaatatgaa tcaagaaata aatctttaga aagtgctgcc atattagagg ctgaaggcgt
     1861 tgactttgca attatgaccg accaccctgt tatacc
//
LOCUS       DATGSI010000062         1696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_571081, whole genome shotgun sequence.
ACCESSION   DATGSI010000062 DATGSI010000000
VERSION     DATGSI010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571081"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..509
                     /locus_tag="VK031_00980"
     CDS             129..509
                     /locus_tag="VK031_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52521.1"
                     /translation="MKEVKTNKKLVNKLNLLVSLLKEEREALIKSDGDKVAEIVKKKE
                     NIIEDLSTFKGLDIREDKIIMELIKEIDSLQEMNLLLTKQALSFQNALLESISKGVKA
                     SLNTYSAKGNYESNNKTGLVDQSV"
     gene            521..>1696
                     /gene="flgK"
                     /locus_tag="VK031_00985"
     CDS             521..>1696
                     /gene="flgK"
                     /locus_tag="VK031_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HLS52522.1"
                     /translation="MSGIFNTLNTATKGLLAQQTSLHTTGHNIANANTEGYSRQRVDL
                     QADIAYHFEGVGQLGTGVKMNSVIRMVDDYVNRQIRQENGSLQKFITKSDVLEQLEII
                     FNEPSDTGLSFNIEEMFVAWQELSKNPESINSKTIVAEKSKTLADTFNHMMNQIDNLT
                     EETIGQIEKDAFDFNSIIDQIRGLNDQIFNISVKGHIPNDLLDQRDMMLKNLSSIMNF
                     DTKFDSYGRVSISVDGETILNEKTELELSVIKDIKENEDGTFTLLVSKGGDSLTEPYV
                     ISGIENIEDFTIGQPVFNRIDEDLNLDNLIASNIKSGKIDGNLESLEEIADRKEGLLA
                     FGEGLAEAINKAHEYTDIFIFEDGKLRINEEIHKDPSLIVAGENPSSPPGDGSRALEI
                     "
BASE COUNT          654 a          249 c          362 g          431 t
ORIGIN      
        1 ggtaaaggac caacaatttg atgaaagagt cgaaaggatc aggcaattgg tagtagaagg
       61 gaaataccct atatcttctg aaaagatagc agaaaggatc ctgcaaacta ttgaagagca
      121 gaagggcagt gaaagaagtg aaaactaata aaaaattagt caataaactt aacctactgg
      181 ttagtttatt aaaggaggaa agagaggccc tgatcaaatc agatggagat aaggtggcag
      241 aaatagtaaa aaagaaggaa aatattatag aagacttgtc tacttttaaa ggcttagata
      301 taagggaaga taagatcatt atggagctaa taaaggagat tgattccctg caggagatga
      361 atctcctttt gactaagcag gccttaagct ttcaaaatgc cttgttagaa agcatttcta
      421 aaggtgtaaa ggccagctta aatacctatt cagcaaaggg aaattacgaa tcaaacaata
      481 agaccggtct ggtggaccag tcagtatagg ggagataaag atgtctggaa tatttaacac
      541 actaaataca gctacaaaag gtttactagc acagcaaact tccttgcata caacaggcca
      601 taatattgct aatgccaata cagagggcta ctcaagacaa agggtagacc tacaggcaga
      661 tattgcctac cattttgaag gagtaggaca attaggcaca ggagtcaaaa tgaattctgt
      721 tatcagaatg gtagatgatt atgttaacag gcagattaga caggaaaatg gaagcctaca
      781 aaagtttatt accaagtctg atgtcttaga acagttggaa ataatcttta atgaaccttc
      841 tgatacaggc ttaagtttta atatagagga aatgtttgtt gcctggcaag aacttagcaa
      901 aaatccagaa agcataaatt ccaaaaccat agtagcagag aaatcaaaaa ccttggctga
      961 tacctttaac catatgatga atcaaattga caacctaact gaagaaacca ttgggcagat
     1021 agaaaaggac gcctttgact tcaattcaat tatagatcaa attaggggat taaatgatca
     1081 aattttcaac atatctgtaa agggccatat acctaacgat ttattggacc aaagggatat
     1141 gatgttgaag aacttatcct ctataatgaa ttttgacacc aagtttgata gctacggaag
     1201 agtaagtatt tctgtagatg gtgaaacaat cttaaatgaa aaaactgagc tagaattaag
     1261 tgttatcaag gatataaagg aaaatgaaga tggaaccttc accctacttg tatctaaggg
     1321 tggggacagc ttaactgaac cttatgtaat atcaggaata gaaaatatag aagactttac
     1381 cataggtcaa cctgtattta atagaattga tgaggattta aatttagaca atctaattgc
     1441 ttccaatata aaatcgggga aaattgatgg aaacttggaa tccttagaag aaatagccga
     1501 cagaaaagaa ggcctgttag ccttcggtga agggttggca gaggcaataa ataaggccca
     1561 tgagtataca gatatattta tatttgaaga tgggaaattg agaataaatg aagaaataca
     1621 caaggatcct agccttattg ttgcaggaga aaatccaagc tcaccgcctg gagatggatc
     1681 tagggcttta gagatt
//
LOCUS       DATGSI010000063         1116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_333547, whole genome shotgun sequence.
ACCESSION   DATGSI010000063 DATGSI010000000
VERSION     DATGSI010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333547"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VK031_00990"
     CDS             complement(<1..256)
                     /locus_tag="VK031_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLS52523.1"
                     /translation="MASYSKEDRRVRRSKRLIAEAFIELMYIKDYDDISVTDIVILAD
                     YNRTTFYRHFENKEDLLYKIVTYYSKEIINSFKKPYKNNNY"
     gene            413..>1116
                     /locus_tag="VK031_00995"
     CDS             413..>1116
                     /locus_tag="VK031_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HLS52524.1"
                     /translation="MTKNWTHSYDVVVVGSGGGGLSAALTAEIFGMSSIVIEKTEVFG
                     GSTAKSGGTIWVPNNTYLQKAGVPDSDEKAKTYMDATVGDRVPEELKNAYLTKGKEMI
                     DFFDKHTKHVRWAYTPGYSDYYPELPGGMPEGRSIEAALFDLRKLGDDLDKMRRAGLP
                     TKGMVLTSYEFHKVNMIMRTWIGKLTSLRVGMRLIRTMLTKYNPTTLGEALVARLYKA
                     LKDEGGDVWVSTPFKDL"
BASE COUNT          365 a          178 c          215 g          358 t
ORIGIN      
        1 cataattatt attcttatag ggttttttaa aggagtttat gatttcctta gagtaatagg
       61 tcactatttt ataaagtaaa tcctctttat tttcaaaatg gcgataaaaa gttgtcctgt
      121 tatagtctgc taagataaca atatctgtaa cggatatgtc gtcatagtcc tttatgtaca
      181 taagttctat aaaagcttca gcaattaatc tcttactcct tctaaccctt ctatcctctt
      241 ttgaataact agccaattat attaccccct ctatctaatt atacaacatt ttccaatatt
      301 ttgttgaata atcacacaaa tgctagcctt ttaatgattg aatgatctaa taaaccgtaa
      361 tacaataaat ttatggacaa ttttaaaaac taatattcta aggggagaga ctatgactaa
      421 aaattggaca catagctatg atgtagtagt agttggttct ggtggtggtg gattatctgc
      481 ggccttaaca gctgagattt tcggaatgtc ctcaattgta attgaaaaga cagaggtatt
      541 cggtgggtcc actgctaaat ccggtggaac aatttgggtt cctaataata cttatttaca
      601 aaaagcaggc gttcctgatt ctgatgaaaa agcaaagact tatatggatg ctacagtcgg
      661 agatcgggtt ccagaggaat taaagaatgc ttacttaaca aaaggtaaag aaatgattga
      721 tttttttgat aagcatacaa aacatgtacg ctgggcttat acgccaggat attctgatta
      781 ttatcctgaa ttacctggag gtatgccaga gggaaggtca attgaagccg ccttatttga
      841 tttaaggaaa ttaggtgacg atctagataa gatgcgaagg gcgggtcttc caaccaaagg
      901 aatggtttta acatcctatg agtttcacaa ggtaaacatg attatgagaa catggattgg
      961 aaaacttact tctttaaggg taggaatgag attaattaga acaatgctca caaagtataa
     1021 tcctacaacc ctcggagaag ctttagttgc ccgattatat aaagccttaa aggatgaggg
     1081 tggcgatgtt tgggtttcta ccccctttaa agacct
//
LOCUS       DATGSI010000064         3998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_333573, whole genome shotgun sequence.
ACCESSION   DATGSI010000064 DATGSI010000000
VERSION     DATGSI010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333573"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2983)
                     /gene="smc"
                     /locus_tag="VK031_01000"
     CDS             complement(<1..2983)
                     /gene="smc"
                     /locus_tag="VK031_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HLS52525.1"
                     /translation="MYLKRIEMQGFKSFADKTTIEFKKDITAIVGPNGSGKSNITDAI
                     RWVLGEQSVKSLRGSKMEDIIFSGTDKRRPLGFAEVTVVFDNRDNAIPIDYNEVAITR
                     RMFRSGESEYYINRNSCRLRDIRELFMDTGVGKEGYSIIGQGRIDEILSNRPEDRRNI
                     FEEAAGIVKYKTRKETAERRLEETEENLIRINDLTHEVNKQFKKLEADSKKATKFINI
                     YEELKTLEANIYLENVKNINKQIDKLKGQLKETRDYISQEEETKEINEEKVKEIQDKI
                     KGLESNVETLREKEISLAKEIDHKKNKISLIEEKENFNRRDINRLKEEIEELEKENLQ
                     LEESIKELKEEASLHKDNLQSTLEIYEHKNEALITINEKISNGEEIINQEKERLMKNY
                     NQISHSKSRLQSLDTLMDGLDARLETLKKELTQYEMEEQNHSHKLKKLTEKKIGKEKA
                     LSTLREEKLKQDRDKESLAREMENLAKDIRNDEIKLRNLNSNLQLYQSMEEGYEGYYR
                     SVKNTLLALEKNPHLRKGFIGTVADLIKVDSLYERAVEVSLGSSLQNIIVERESDAKK
                     LIEYLKEKKLGRVTFLPKTTIKGRILDINIEIMKEYGVLGLGHQVIDFPKQYQNIFEY
                     LLGRTIVVDNIDNAISLSNRFGYIYRLVTLEGDIINPGGSLTGGSLNKSISLISRKNR
                     IKEFKKEIEIVTETLEEKERKNTYLTEQLEAQLKNIIQLEEKIKEEELDLLKLSNEVE
                     LLNRELINFKEQILNITEHIGRTKEEKDSYSEEKKIEELSLNRLTEESANIKINIDEL
                     SKEFEDELSLRNKLLQNVTDIRLKTNNLENSISNLEKEISSKEKETEKNKQLIENKNH
                     AILNIEEKSGQLKKQKELLLLEISNKSKELLTVKKDLEFIIPKKEDITNKLHQKQALL
                     EETNKLINKLEKEINSDQLKLSKLEMELEYLDSRLADSYELNYQEALKYKQDIENMKE
                     TKERVNKLKGQLKRLGNVNLTS"
     gene            complement(3009..>3998)
                     /locus_tag="VK031_01005"
     CDS             complement(3009..>3998)
                     /locus_tag="VK031_01005"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLS52526.1"
                     /translation="IKREKVVDTIERYLSYFNRKALIEIAFYGGSFTAIDLSIQNQLL
                     SLASTYKKKGLIDEIRISTRPDAITPDILDNLIKYQVNTIELGVQSLDKEVLRASARG
                     HSCEEVYEAVELIRSYGFKLGLQMMIGLPTDNREKVWKTALQFVDMRPDYVRIYPTLV
                     IRDTYLERLTDLNKYKPLSIKEAVDITTPLLALFYINEINVIRVGLQATENIQLGKDV
                     VAGPFHPAFRQLVESNLYREIIDFYLGKKKVNTLGESFEIFANEKEISNIVGQGSTNI
                     SYFNGKYGFLRTKIYKGDVGPNNLDFKIGSYRETFNRLTLMEKYLETKLKYKD"
BASE COUNT         1002 a          826 c          481 g         1689 t
ORIGIN      
        1 aggaggtcag atttacattt cccaatctct ttaattgacc ctttaattta ttcactcttt
       61 ctttcgtctc tttcatattc tctatatctt gtttgtattt taaggcctcc tggtaattta
      121 actcgtaact atcagccaac ctggaatcca gatattctag ctccatctcc aattttgata
      181 attttaactg atcactgtta atttctttct ccaatttatt tattaattta ttagtctcct
      241 ctagtagagc ctgtttttga tgaagcttat ttgtaatatc ttcttttttt ggaattataa
      301 actcaagatc ctttttaaca gttaatagtt ccttactctt attagagatt tccaataaaa
      361 gtaattcttt ttgtttcttt agctggcctg atttttcttc aatgtttaat attgcatgat
      421 ttttattttc tattagctgt ttatttttct ctgtttcctt ttctttagat gaaatctctt
      481 tttccaggtt agaaattgaa ttctccaaat tatttgtttt taacctaatg tctgttacat
      541 tctgtaaaag tttgttacgt aagctcaact catcttcaaa ctctttggac aattcatcaa
      601 tatttatttt tatattggca ctttcttcag tcagcctatt taaagatagt tcttctattt
      661 tcttctcctc tgagtaagaa tctttttctt ccttggttcg ccctatgtgc tcggttatat
      721 ttaggatttg ttctttaaaa tttattaatt ctctattgag cagttctact tcattggata
      781 attttaaaag atccaattct tcttccttta ttttttcctc caactggatt atgtttttta
      841 actgggcttc taattgttct gttaaatagg tgtttttcct ttccttttcc tccaaagttt
      901 ctgttacaat ttctatctct tttttaaatt ccttaatcct attcttcctg ctgattagac
      961 ttatagactt attcaggcta cccccagtta aggatcctcc aggatttata atatctcctt
     1021 ctaaggtgac cagcctgtat atatatccaa atctattgga tagacttatg gcattatcaa
     1081 tattatcaac cacaattgtc ctacccaaca agtattcaaa tatgttttgg tattgctttg
     1141 gaaaatctat tacctggtga cctaaaccta aaactccata ttctttcata atttcaatat
     1201 tgatatctaa tattctccct ttaatggtgg tcttaggcag gaaggttacc ctccccagct
     1261 ttttttcttt caagtattct attaatttct ttgcatcact ttccctttct acaattatat
     1321 tttgtagact acttcctaag cttacttcta cagccctttc ataaagacta tcaaccttaa
     1381 ttaaatcagc taccgtacct ataaacccct ttcttaaatg cgggtttttt tccaaggcca
     1441 ataaagtgtt tttaacactt ctatagtatc cttcataacc ttcctccata ctttggtaaa
     1501 gctgtaaatt tgaatttagg tttcttaatt tgatttcatc atttcttata tccttagcca
     1561 agttttccat ttccctggct aagctttcct tatccctatc ctgctttaat ttttcttccc
     1621 ttaaagtaga taaggccttt tcttttccta tctttttttc cgttaatttt ttcaacttat
     1681 ggctatggtt ttgttcttcc atctcatatt gggtaagttc tttttttaag gtttccagcc
     1741 tagcatccag cccatccata agggtatcta aactttgcaa cctgctctta ctatgggaaa
     1801 tttgattgta gtttttcatt aatctttctt tttcctggtt tataatctct tccccattgc
     1861 ttattttttc gtttatagta attagagctt catttttatg ttcatatatt tctaaagttg
     1921 attgaagatt atccttgtgt aaagatgcct cttctttcaa ttccttaatt gattcttcca
     1981 gttgtagatt ttctttctct aattcttcaa tttcttcctt taacctattt atatctcttc
     2041 tgttaaagtt ttctttttcc tcaatcagtg aaattttgtt tttcttatga tcaatttctt
     2101 ttgccaggct tatttccttc tctctaagag tttccacatt tgattccagg ccctttatct
     2161 tatcttgaat ttctttgacc ttctcttcat ttatctcttt tgtctcctct tcctgactta
     2221 tgtaatctct ggtctccttt aattgccctt ttagtttatc tatctgctta tttatatttt
     2281 tcacattttc taaatatata ttagcctcta gggtcttcag ctcttcataa atattaataa
     2341 atttggtggc ctttttagaa tctgcttcaa gttttttaaa ttgtttatta acctcatggg
     2401 ttaagtcatt tattctaatt aagttttcct ctgtttcttc aagccttctt tctgcagtct
     2461 cttttcttgt tttatattta acgatccctg cagcctcttc aaatatattt ctcctatctt
     2521 caggcctatt acttaagatt tcatctattc tcccctgccc tataatagag taaccttctt
     2581 tgcctactcc tgtatccata aatagctccc taatatccct tagcctacag gaatttctat
     2641 ttatataata ttcgctttcg ccagaccgaa acatcctcct tgtgattgca acctcattgt
     2701 aatctatagg aatagcatta tccctgttat caaaaacaac tgttacctct gcaaaaccca
     2761 ggggccttct tttatctgtt cctgaaaaaa taatatcttc cattttactt ccacgtaaac
     2821 tttttacact ctgttcgcct agaacccatc tgattgcatc tgtgatatta ctttttccac
     2881 tgccattagg gcctacaatt gctgttatat cctttttaaa ttctatcgtt gttttatctg
     2941 caaaggattt aaagccttgc atttctattc ttttcaagta caaatttaac acccctgtaa
     3001 tcttaaaatt aatctttata ttttagcttt gtctctagat atttttccat aagggttaat
     3061 ctattaaatg tttctctgta agaacctatt ttaaaatcca aattattagg gcctacatct
     3121 cccttgtaaa ttttagtcct taaaaaccca tatttaccat taaaataact tatatttgta
     3181 gatccctgtc ctactatatt ggagatttcc ttttcattgg caaaaatctc aaaactttca
     3241 cctaaggtat ttaccttctt ttttcctagg taaaagtcta ttatttctct atataggttt
     3301 gattctacca gttgcctgaa agcagggtga aaaggacctg ctaccacgtc tttccccagt
     3361 tgaatgtttt ctgtggcctg taggcctact cttataacat ttatctcatt tatataaaaa
     3421 agggccaaaa ggggagttgt tatatctaca gcctctttta tgctaagagg cttgtactta
     3481 tttaaatcag ttaatctttc caggtaggta tctcttataa ctagggtagg gtaaatccta
     3541 acataatcag gcctcatatc tacaaattgt agggccgtct tccacacctt ctccctgtta
     3601 tctgtaggca gtcctatcat catttgaagg ccaagtttaa atccatatga cctaattaat
     3661 tccactgctt catatacttc ttcacagctg tgtcctctgg cactggccct tagaacctct
     3721 ttatctagcg actggacacc cagttcaatg gtatttacct ggtatttaat taaattatct
     3781 aatatgtcag gagtaattgc atcaggccta gtggatatcc taatttcatc aattaggcct
     3841 ttcttcttgt aggttgaagc caaacttaat aattggtttt gaatagataa gtctatagcg
     3901 gtaaaacttc ctccgtaaaa agcaatttca ataagggcct tcctattaaa gtagcttaga
     3961 tacctttcaa tagtatctac aactttctct ctttttat
//
LOCUS       DATGSI010000065         1243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_571210, whole genome shotgun sequence.
ACCESSION   DATGSI010000065 DATGSI010000000
VERSION     DATGSI010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571210"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VK031_01010"
     CDS             <1..736
                     /locus_tag="VK031_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HLS52527.1"
                     /translation="TGYNIVDVEATKKKGIVVTNVPDYGTNTVAQMTFALLLELTNHV
                     GHHNQKVKEGKWTQSLDWCFWEKSLVELSDKTIGILGYGRIGRKVAEIARAFGMKVIA
                     TVNNPTKRASEDNVEFVNADTLFTQSDVISLHCPLTPETKGIINKDSIEKMKSNAIII
                     NTARGPLIIEDDLADALGKGKIAGAAVDVASVEPIDAGNPLLKAKNCIVTPHIAWATR
                     EARIRLLNSAIENLEMFLKGSPINRV"
     gene            889..>1243
                     /locus_tag="VK031_01015"
     CDS             889..>1243
                     /locus_tag="VK031_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HLS52528.1"
                     /translation="MDYRIEHDTMGEVRVPIEKYWGAQTQRSKENFKIGIEKMPIEVI
                     RAFSILKKAAAITNNKLGRLDDKRSKIIGQVCDEIHEGELDDDFPLVVWQTGSGTQSN
                     MNVNEVVANRANEILG"
BASE COUNT          469 a          176 c          274 g          324 t
ORIGIN      
        1 aacaggttac aatatagtag atgtagaggc aaccaaaaag aagggaatag tagttacaaa
       61 tgtaccagat tatggaacaa acacagtagc acagatgaca tttgccctat tactggagtt
      121 aaccaatcat gtaggtcacc ataatcaaaa agttaaagag ggaaagtgga ctcaatctct
      181 agattggtgt ttctgggaaa aatctctagt tgaattatca gataaaacta ttggtatttt
      241 aggctatgga aggattggaa gaaaggtagc tgagatagca agggcatttg gtatgaaggt
      301 tattgcaacc gttaataatc caacaaaaag agcatcagaa gacaatgtag aatttgtaaa
      361 tgcagatact ttgtttaccc aatcagatgt gatcagtctc cattgtccct taacacctga
      421 aaccaaaggc attataaata aggattctat agagaaaatg aaatcaaatg ccattattat
      481 aaatacagcc aggggtccct tgataattga ggatgatttg gcagatgctc tagggaaagg
      541 taaaatagca ggggctgctg ttgatgtagc atctgtggaa cctatagatg caggaaatcc
      601 attattaaag gcgaaaaact gcattgtaac cccccacata gcatgggcta cgagggaagc
      661 aagaattaga ttgctaaata gtgcaataga aaacctagaa atgtttctaa aaggtagtcc
      721 tataaatcga gtataataat taactcctta tatacaaatc taaacagaac tctatcggag
      781 aaaaactgat cgggttctgt tttttacaaa ttatccactt gaaaactttt taatccctct
      841 agttcatggg tataggctat atgaaagata aaaaacggag gggtttatat ggattatagg
      901 attgaacatg acaccatggg ggaagttagg gtacctatag aaaaatactg gggtgctcaa
      961 acccaaagga gtaaggaaaa ctttaagata ggcatagaaa aaatgcctat agaagtaatc
     1021 agagcattta gtattttaaa aaaggcagca gcaattacta ataacaaatt aggtagacta
     1081 gatgataaaa gaagcaagat aataggccag gtatgtgatg agattcatga gggagaattg
     1141 gatgatgatt ttcccttggt agtatggcag accggtagtg gaacccaaag taatatgaat
     1201 gtaaatgaag ttgtcgccaa tagggctaat gaaattttag gaa
//
LOCUS       DATGSI010000066         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_428147, whole genome shotgun sequence.
ACCESSION   DATGSI010000066 DATGSI010000000
VERSION     DATGSI010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428147"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1221)
                     /locus_tag="VK031_01020"
     CDS             complement(118..1221)
                     /locus_tag="VK031_01020"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HLS52529.1"
                     /translation="MDTYKDLDTPALLLDREKLMRNIQFVQAYADQYKVSLRPHTKTH
                     KMPYLAKLQVEAGAGGITVAKVGEAEVMAKAGIKDIFIANEIVGKQKLKRIKKLSKEI
                     NISFGVDSIEHVKTIEDIFDKENKAEILIEIEVGDKRTGIIRKKDFLDLVKYIKTCKN
                     IRLKGIFSHEGHTYAVKNLDELKEAFFESQKKTLSFAKLSEDLGEKLEVVSIGATPPT
                     IHRLPILEGVTEIRPGTYILMDMGQANALGSHDRCAATILSTIISKPTSERVVTDVGA
                     KGLTMQKRDKGICATKGLGYIKDYSVYIDKLNDEHAIIYDKEFNRQVKIGDKVEIIPN
                     HICPVCNLYDRAYLVSDGKVIKRIEISCRGKMT"
     gene            complement(1310..1729)
                     /locus_tag="VK031_01025"
     CDS             complement(1310..1729)
                     /locus_tag="VK031_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HLS52530.1"
                     /translation="MEYKRFQDKYLVRLERGEEIVESIEKLAKKENIRLGKVSGIGAV
                     NKATIGLFKADTKEYKSTELTGDMEIVNLTGNISEMDGEVYLHLHIALGDDKYNLYGG
                     HLNSATISVTGELIIDVMEGQVDREYNEEIGLNLLKF"
     gene            complement(1824..>2160)
                     /locus_tag="VK031_01030"
     CDS             complement(1824..>2160)
                     /locus_tag="VK031_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52531.1"
                     /translation="GINNTFIALSHPKTIVIDKEGIEFHSFGRVHSYSWARLDYFMVK
                     EFSARKLYLRIENPKLFKGRYWIKTNMYNDSDELYLKINKEEKRIHPNLLKFKAKKRR
                     PNKKKRRKK"
BASE COUNT          618 a          465 c          248 g          829 t
ORIGIN      
        1 aaaaaataaa cggcttataa cctattaaat ataggaataa gccgcataat tcatattgtc
       61 ttaaggttta aattaaaaac tatcatcagc ttaactctct tgaggaaaat tagctcacta
      121 agtcattttc cctctacatg atatttcaat cctttttatt actttgccat ctgagactag
      181 ataggcccta tcatacaaat tacatacagg acatatgtgg ttaggtatta tttctacttt
      241 atcacctatt tttacctgcc tattaaattc tttatcataa ataattgcat gttcatcatt
      301 taatttatct atatatacag aataatcctt tatataacct aatcccttgg ttgcacatat
      361 tcctttatcc cttttctgca tggtaaggcc cttggccccc acatctgtaa ctactctttc
      421 actggtaggc ttactaatta tagtactcag tatggttgct gcacatctat catggctgcc
      481 tagagcattg gcctgtccca tatccattaa tatataggtt cctggcctta tttctgtaac
      541 cccttctaat ataggtagcc tatgtatagt aggtggtgtg gcacctatac ttaccacctc
      601 taacttttct cctaagtcct ctgaaagttt tgcaaagctt aaagttttct tctgactttc
      661 aaaaaaagct tcctttaatt catctaaatt ttttactgca taagtatgtc cttcgtgaga
      721 aaatatccct tttagcctaa tatttttaca agttttaata tactttacta gatctaaaaa
      781 atcctttttt ctgataattc cagtcctttt atccccaacc tcaatttcta ttaaaatttc
      841 tgccttattc tccttatcaa atatatcctc tatggtcttt acatgctcta tagagtctac
      901 accaaaggat atgttaattt cttttgataa tttttttatt cttttcaact tttgctttcc
      961 aacaatttca ttggcaataa agatatcctt aatacctgcc ttagccataa cttctgcctc
     1021 tcccacctta gccacagtga ttcccccagc tccagcttcc acttgtaact tggctaaata
     1081 aggcatttta tgcgtcttag tatggggcct tagacttacc ttatattggt cagcataggc
     1141 ttgaacaaat tggatatttc tcattaattt ttccctatca agaagtaggg ccggagtatc
     1201 caaatcctta taagtatcca tttaatcacc ctttatcttt taattacatt atatacttaa
     1261 cccgccaatt aacaacaatt aaaaaagcag ggtttccacc ctgcatatat tagaacttaa
     1321 gaagattcaa tccaatctct tcattgtatt ctctatcaac ttggccttcc attacatcta
     1381 ttataagttc accggttaca cttatggtgg cagagtttaa atgaccccca tataaattgt
     1441 atttatcatc tcctaaggca atatgaaggt gtagatacac ttctccatcc atttcagaaa
     1501 tatttcctgt taaattgact atttccatat ctcctgttag ttctgtagat ttatactcct
     1561 ttgtgtctgc tttaaaaagg cctatggttg ccttattaac tgcaccaatt cccgatacct
     1621 ttcctaacct tatattttct ttcttagcca acttttctat actttctact atctcttctc
     1681 ccctttccaa cctgacaaga tacttgtctt gaaatctttt atactccatg aaatcactcc
     1741 ttaataactc ttcatagata aattataccc agaaaatagt ttttaagcgt aattcacact
     1801 tttcccctat ttaacgattg tttctatttt ttccgccttt tctttttatt aggtcttctt
     1861 tttttagcct taaattttaa caaatttgga tggattcttt tctcttcttt atttattttt
     1921 aaataaagct catcactatc attatacata ttagttttta tccaatacct acccttaaat
     1981 aatttagggt tttcgattct tagatataat tttctggccg aaaactcctt taccataaaa
     2041 taatctagcc tagcccaaga ataggaatga acccgtccaa aggaatggaa ctctattccc
     2101 tctttatcaa taacaattgt tttaggatgg cttaaggcaa taaaggtatt atttattcca
//
LOCUS       DATGSI010000067         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_240675, whole genome shotgun sequence.
ACCESSION   DATGSI010000067 DATGSI010000000
VERSION     DATGSI010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240675"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VK031_01035"
     CDS             <1..801
                     /locus_tag="VK031_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HLS52532.1"
                     /translation="SLLIWIGELITEKGIGNGISLIIFVGIISRIPGSLINIFKLVRI
                     GELKILQVLIFGLVFVAIIAVVVALQEGERRIPVQYAKRVVGRKMYGGQSTHIPIKVS
                     MSGVMPVIFASSFLALPQTLALFFKGGVSDWITKYLTPQGSVGIWIYSILNIALIMGF
                     TYFYTAVQFNTVEYAKNLQQNGGFIPGIRPGRPTSEALNRVVSRITFVGGLSLAVLAS
                     LPIILSKIFRMNVNFGGTAVIIVVGVALETVKQIESQMLMRHYKGFLK"
     gene            820..1467
                     /locus_tag="VK031_01040"
     CDS             820..1467
                     /locus_tag="VK031_01040"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HLS52533.1"
                     /translation="MRLVLLGPPGVGKGTQASNIVEKYNIPHISTGDIFRANIKEGTE
                     LGKTAKEYMDKGLLVPDEIVVSIVKDRISQKDCENGFLLDGFPRTVEQGKALDEELSN
                     MGIKLDKVVNIEASEDVLIERITGRRICKQCGATYHVKNKPPKVDGICDIDGGTLYQR
                     EDDKVETVATRIQVYQKQTQPLIDYYKNKGLLLEIDGTRAIEDIFNTIVNSLEDN"
     gene            1528..1800
                     /locus_tag="VK031_01045"
     CDS             1528..1800
                     /locus_tag="VK031_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HLS52534.1"
                     /translation="MKTTSDIAIGQVVKSKAGRDKGRVFLVLEIIDEHHVYIVDGDLR
                     KLDRPKKKKVKHLDIYNTVQTELKYKKDNNIKLNNAFIRKLLEPFS"
     gene            1838..2056
                     /gene="infA"
                     /locus_tag="VK031_01050"
     CDS             1838..2056
                     /gene="infA"
                     /locus_tag="VK031_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HLS52535.1"
                     /translation="MAKKDVIEVEGTVTEALPNAIFKVKLENDHEILAHISGKLRMNY
                     IRILPGDKVTVELSPYDLTRGRITWRKK"
     gene            2073..2186
                     /gene="rpmJ"
                     /locus_tag="VK031_01055"
     CDS             2073..2186
                     /gene="rpmJ"
                     /locus_tag="VK031_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HLS52536.1"
                     /translation="MKVRPSVKKMCEKCKIIRRRGRVMVICENPKHKQRQG"
BASE COUNT          810 a          293 c          503 g          636 t
ORIGIN      
        1 tccctattaa tttggatagg tgaattgatc actgaaaaag gtataggtaa tggtatatcc
       61 ttgataattt ttgtaggaat tatctcaagg ataccaggaa gcttaattaa tatatttaag
      121 ctggtaagga tcggagaatt aaaaattctg caagtcctaa tatttgggct agtatttgtt
      181 gcaattattg cagtagtagt ggctctacaa gaaggggaaa gaaggattcc agtacaatat
      241 gcaaagaggg tagtaggcag aaaaatgtat gggggacaga gcacccatat accaattaag
      301 gtttcaatgt ctggtgttat gcctgtaatc tttgcttcat ctttcttagc attaccacag
      361 accttggccc tattctttaa aggaggagta tcagattgga ttacaaagta ccttactcct
      421 caaggcagtg taggaatatg gatatattct attttaaata tagcactaat aatgggcttt
      481 acctattttt acacagctgt ccagtttaat actgtagaat atgccaagaa cctacaacaa
      541 aatggtggtt ttattccagg cataaggcca ggaagaccaa catcagaagc attaaacagg
      601 gtagtatcaa ggataacttt tgtaggtggt ctatctttgg cagtattagc atcattaccg
      661 attatccttt caaaaatatt caggatgaat gttaatttcg gtggtactgc tgttattatt
      721 gtggtaggag tagccttaga gacagttaaa caaatagagt cacaaatgtt aatgagacat
      781 tataaaggct ttttaaaata gactatagga gatgaaaaga tgagactggt attattagga
      841 ccaccaggtg tgggtaaggg gactcaagca tcaaatatag tagaaaaata taatataccc
      901 catatatcta cgggagacat ctttagagct aatataaagg aaggtacaga gctgggaaaa
      961 acagcaaaag aatatatgga taaggggctt ttagtgcctg atgaaatagt agtttccata
     1021 gtgaaggata ggattagcca aaaggattgt gaaaatggat ttttattaga tggattccct
     1081 agaacagtag agcaaggcaa ggctctagat gaggaattat ctaatatggg tataaaattg
     1141 gataaggttg tcaatattga agcaagtgag gacgttttaa ttgaaagaat aacaggtaga
     1201 aggatttgta agcagtgcgg tgcaacctat catgttaaaa ataagcctcc aaaggtagac
     1261 ggcatatgtg atattgatgg tggaacctta taccaaaggg aagatgataa ggttgaaact
     1321 gtagcaacta ggattcaagt ataccagaag cagacccaac ctttaataga ctattataag
     1381 aataaaggct tgcttttaga aatagatggt accagggcta tagaagacat atttaatact
     1441 atagtcaatt ccctagaaga taattaatag ctctaggcta ttaaaaaatg ctacaggctg
     1501 ccaagtcaac ttaaagaggt gaagttaatg aaaacaacta gcgacatagc cattgggcaa
     1561 gtggttaagt caaaagctgg tcgtgataag ggaagagttt tcttggtgct tgaaataata
     1621 gatgaacatc atgtttatat tgtagatggc gatttaagaa aacttgatag gcctaagaaa
     1681 aagaaggtaa aacatttaga tatctacaat acagtgcaaa cagaacttaa gtataagaaa
     1741 gataataata taaagcttaa taatgctttt ataaggaagc ttttagagcc ttttagctaa
     1801 ggttctaaat tcgttgaaca ggaggttcga aaactttatg gctaaaaaag atgttattga
     1861 ggtagaaggt acagttacag aagctttacc taatgccata tttaaagtga agcttgaaaa
     1921 tgatcacgag attttagccc atatttctgg gaaattaaga atgaactata taaggatact
     1981 tcctggggat aaagtaactg ttgaactctc accctacgat ttgacaagag gtagaataac
     2041 ttggcgaaag aagtagcata ggagggattg atatgaaagt aagaccatcg gttaagaaga
     2101 tgtgtgaaaa atgtaagatt atcagaagac gtggtagggt tatggtaatc tgtgagaatc
     2161 cgaaacataa acaaagacaa ggttaattta tagatgcaca ggaggtgtgg ttttaatggc
     2221 aagaattgca ggtgttgact ta
//
LOCUS       DATGSI010000068         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_52268, whole genome shotgun sequence.
ACCESSION   DATGSI010000068 DATGSI010000000
VERSION     DATGSI010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52268"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1033)
                     /locus_tag="VK031_01060"
     CDS             complement(29..1033)
                     /locus_tag="VK031_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HLS52537.1"
                     /translation="MGLISWLNLHLLKMQWLWDLVEYLLVQIFGLSMEDKIGASIHFF
                     IYDVIKIFILLSVMIFIISYIQSYFPPDRTRKLLGGIKGIKGNILGAVMGILTPFCSC
                     SSIPIFIGFVSAGLPLGATFSFLISSPMIDLGALMLLMSTFGGKVGLVYVLSGVTVAI
                     IGGIIIQKANMEGYVESYVWVVESADTEIEQLDYKDRIDFAKGEVKSIFFKVWPYILI
                     GVAIGAGIHNWIPQSIIEKILGRSNPLSVLIATFIGVPIYADIFGTIPIAEALVAKGV
                     GIGTVISFMMGVTTMSIPSMVMLSKVIKPKLLGTFILITTIGIILVGYGLNGFSFLLV
                     "
     gene            complement(1055..1456)
                     /locus_tag="VK031_01065"
     CDS             complement(1055..1456)
                     /locus_tag="VK031_01065"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HLS52538.1"
                     /translation="MKKKVAFVCVHNSCRSQMAEGWAKRLGSHLLEVYSAGTEDYPEV
                     KPLAVKVMKEANVDISSHRPKLLEDIPEKVDILITMGCNVVCPSIVSNYYEDWGLKDP
                     SGGPIEDYRTTRDKIKSRVEQLIDMVDQGKI"
     gene            complement(1453..1773)
                     /locus_tag="VK031_01070"
     CDS             complement(1453..1773)
                     /locus_tag="VK031_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLS52539.1"
                     /translation="MTNYAENARIFKVLSDPNRLKILDILSCGERCVCDILEFFDFTQ
                     PTLSYHMKVLMEAGLVNVRREGLWSHYSLNKENCNRLIGVLMNMVNDTNSCICKNIRK
                     EVTK"
     gene            complement(1887..>2218)
                     /locus_tag="VK031_01075"
     CDS             complement(1887..>2218)
                     /locus_tag="VK031_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HLS52540.1"
                     /translation="HVDGVMLGRVAYENPFLLTKVDQFFDNNYINTTTRKDIILKLID
                     YVDSLEGNERTEHQALRNTLGLFHKKRGSKIWRQLLTPPWPKGYRASDVLKKALEEIP
                     EEILIER"
BASE COUNT          693 a          476 c          312 g          737 t
ORIGIN      
        1 atttttatta ccatttatat ccctcctttt acactaacaa aaatgaaaaa ccattaagtc
       61 catagcctac taaaataatc ccaatagtag ttattaagat aaaggttccc agtaatttag
      121 gtttaattac cttgcttagc atcaccatag atggaatgga catggttgtt actcccatca
      181 taaaggagat aacagttcct atgcctacac ccttggccac tagagcctca gctataggta
      241 ttgttccaaa aatgtctgca taaataggta ctcctataaa agtggctatt aggacagaca
      301 agggattgct acgacctagg attttttcaa ttattgattg gggaatccaa ttgtgaatcc
      361 cagcccctat ggctactcct attaatatat agggccagac cttaaaaaat atggacttta
      421 cctctccctt ggcaaaatca atcctatctt tatagtccaa ctgttcaatc tctgtgtcag
      481 ctgactccac aacccataca taggactcta cataaccttc catatttgct ttttggatta
      541 taattccacc tattattgca acagttacac cggaaagtac atataccaag cctacttttc
      601 ctccaaaggt ggacataagt aacataagcg cccctaagtc tatcataggc gatgaaatta
      661 aaaaggaaaa ggtggctccc aagggaagtc cagctgatac aaagcctata aatataggta
      721 tgctggagca ggagcaaaag ggtgtcaata ttcccattac agcccctaaa atattccctt
      781 taatcccttt tatgccccct agtaattttc tggttctatc cggtggaaaa taggattgta
      841 tataggaaat aataaaaatc attacagaaa gtaggataaa tatttttatt acgtcataaa
      901 taaaaaaatg gatactagcc cctatcttat cttccattga aagtccaaat atctggacaa
      961 gtaaatattc tactaaatcc cacaaccact gcatctttaa caaatgtagg tttaaccaac
     1021 taatcagccc cataacttca tgtcatcctt tcaattaaat cttaccttgg tctaccatat
     1081 caattaattg ttcaacccta ctttttatct tatctcttgt tgttctatag tcttcaattg
     1141 gcccgccgga agggtctttg aggccccaat cctcataata gttgctaact atagatggac
     1201 agactacatt gcatcccata gtaattaaaa tatctacctt ctctggaata tcttctaata
     1261 gttttggcct atgagaactt atatcaacat ttgcttcttt cattaccttt actgccaaag
     1321 gtttaacttc tggataatct tctgttcctg cagaataaac ctctaaaaga tgactgccta
     1381 atcttttagc ccagccttct gccatctgag atctacaaga attatggaca caaacaaagg
     1441 ctactttttt cttcattttg ttacctcttt tctaatattt ttacaaatac aactattggt
     1501 gtcattgacc atattcatta aaactccaat gagcctatta caattttcct tgtttaaaga
     1561 atagtggctc cacaatcctt ctctcctaac atttaccaag ccagcttcca ttaatacctt
     1621 catatggtag gaaagggttg gctgtgtaaa gtcaaaaaat tccagtatgt cacaaacaca
     1681 tctttcacca caagataaaa tatctaaaat tttcaacctg ttaggatcgg ataaaacctt
     1741 aaatattctt gcattttccg cataatttgt catcttatcc ctccttatat agatagatgt
     1801 ctatatagtt attgtataga tagttatcta tatagtcaag aaaaaaatgt tacaatttat
     1861 aaattgtaac attttttatc agctctttac ctttctatca aaatctcctc tgggatttcc
     1921 tccagagcct ttttaagcac atcacttgcc ctataaccct tgggccaggg tggagttaaa
     1981 agttgtctcc agattttact gcctcttttc ttatggaaaa gtcctagggt gtttctcaag
     2041 gcttggtgtt ctgttctttc attaccttcc aaactatcca catagtctat taactttaaa
     2101 ataatatctt ttctggtagt tgtatttata tagttgttat caaaaaactg atctaccttg
     2161 gttaaaagaa aagggttttc ataggctact ctccccagca taaccccgtc cacatgtt
//
LOCUS       DATGSI010000069         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_52579, whole genome shotgun sequence.
ACCESSION   DATGSI010000069 DATGSI010000000
VERSION     DATGSI010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52579"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VK031_01080"
     CDS             <1..1026
                     /locus_tag="VK031_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS52541.1"
                     /translation="THGRELVAKYLESKKGFILVSHDREFINSIVDHVLSIEKNKIVL
                     QKGSYDTWEANKNLEDQYELEKNQKLRKEIRRLKESAREKALWSDKIEATKIGSGPVD
                     RGYIGHMAAKMMKRSKSIEKRYEKAIKEKEKLLKNIEPIEKLQINNLEHHARPYINVE
                     NFTISYDDKEIFRPIDFTVEKGDCILLKGDNGSGKSSIIKAILGRRVPWKGKLEIAKG
                     ITISYVPQKFHYVKGNINDFVNREGLDKTMFLTILRKLGFSRNQFEIAIENFSMGQKK
                     KIFLAKSICEEAHIFIWDEPLNYIDVISRIQIENMILEYSPTMILVEHDSRFIDKVAT
                     DIIELSR"
BASE COUNT          435 a          130 c          208 g          278 t
ORIGIN      
        1 acccatggta gagagctagt agcaaaatat ttagaaagta aaaaaggctt tatccttgta
       61 tcccatgata gggagtttat aaattctata gtggatcatg tattaagtat agaaaaaaat
      121 aaaattgtcc ttcagaaggg aagctatgat acctgggagg ctaataagaa tcttgaagac
      181 cagtatgaat tagaaaaaaa tcaaaaatta cgcaaggaaa taagaagatt aaaagaatcg
      241 gcaagggaaa aggctctatg gtctgataag attgaggcca ctaaaattgg ctcaggtcct
      301 gtggacaggg gctatatagg ccatatggca gctaaaatga tgaagcgttc caagtctata
      361 gagaaaagat atgaaaaagc aataaaagaa aaagagaaac tacttaaaaa tatagagcct
      421 attgagaaac tacaaataaa caatttagaa catcatgcta gaccatatat aaatgtggag
      481 aactttacaa tttcttatga tgataaagag atttttaggc ctatagattt tacagtggaa
      541 aagggagatt gtatcctatt aaagggagac aatggtagtg gaaaaagcag cataataaaa
      601 gcaattttag gaagaagagt accttggaaa ggcaaacttg aaattgccaa gggaatcacc
      661 atatcctacg tgccccagaa atttcactat gttaagggaa atataaatga ttttgtaaac
      721 agagaaggac tagataagac catgttttta actatattga gaaagctagg attttcaaga
      781 aatcagtttg aaatagctat agaaaacttt tctatggggc agaagaaaaa aatcttccta
      841 gctaagtcca tatgtgaaga agcacatata tttatctggg atgaaccttt aaattatatt
      901 gatgtaatat ccagaataca aatagaaaat atgatacttg aatattctcc tactatgata
      961 ctagtagaac atgatagcag atttatagat aaggtagcta cagatattat agaattatca
     1021 agataggata aagatttatg gacataaaaa c
//
LOCUS       DATGSI010000070         1623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_52801, whole genome shotgun sequence.
ACCESSION   DATGSI010000070 DATGSI010000000
VERSION     DATGSI010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52801"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1034
                     /gene="spo0A"
                     /locus_tag="VK031_01085"
     CDS             228..1034
                     /gene="spo0A"
                     /locus_tag="VK031_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HLS52542.1"
                     /translation="MEKIRIVIADDNREFCQLISKFLSSQEEFEVVGIAKDGIETLEI
                     VEKEKPDILVLDVIMPHLDGLGVLERLNQINGEYSPRVVMLSAVGQDKITQRAIELGA
                     SYYMVKPFDFNIFIERLRQVSDVKNRKLAPSFSENLMEQNHMNQEKNLETRVTKIIQE
                     IGIPAHIKGYLYLREAITIVIHDIDYLGAITKELYPAVADKFNTTPSRVERAIRHAIE
                     VAWNRGKIETLNKIFGYTVSTDRGKPTNSEFIALISDKLRLEFSDLGGIS"
     gene            1130..>1623
                     /locus_tag="VK031_01090"
     CDS             1130..>1623
                     /locus_tag="VK031_01090"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HLS52543.1"
                     /translation="MDYQEKTMKTDKLYEGKILNLRIDTVELPNKKYSKREIVEHAGS
                     VAIIPMISDDSLVLVRQYRKAVEDFLLEVPAGRLELNEEPRETAIRELKEETGLNTKK
                     LEYISEFYASPGYCNEKIYLFLAKDLFEDKEFIQDRDEEYIEKEIYTFDQLLSMIKKG
                     DIVD"
BASE COUNT          613 a          190 c          323 g          497 t
ORIGIN      
        1 tgtatttgta aatgatccat ctaaagggta tggtttatat attgaatgga tgttgaatca
       61 agaagatagg ttttaaaacc tatcttcttt ttagctaatt ataaaattta agaaggcaga
      121 taagcgaata tttggaaata taagaaaaat ataataaaaa agaaggactt ttaattttaa
      181 tgtcgaaata atatagaaat agagtatgtc caggggggag attttttatg gaaaaaataa
      241 ggatagttat tgcagatgac aatcgagagt tttgtcagtt gatttcaaaa tttttgtcta
      301 gtcaagagga gtttgaagtt gtggggattg ccaaagatgg aatagaaact ttggaaatag
      361 ttgaaaaaga aaagccagat attttagttt tagatgtaat catgcctcat ctagatggtt
      421 taggtgtatt ggaaagatta aatcagatta atggggaata ttcaccgagg gttgttatgt
      481 tatctgctgt aggccaggat aaaataacgc agagagccat agaattaggt gcctcctatt
      541 atatggtaaa gccttttgac ttcaatatat ttatagaaag attaagacag gtctcagatg
      601 taaagaatag aaaacttgct cccagctttt cagaaaattt gatggaacaa aatcatatga
      661 accaagaaaa aaatttagag acaagagtta ctaaaattat acaagaaata ggtattcctg
      721 ctcatattaa aggatatcta tacttaagag aggcaattac aatagttatt catgatatag
      781 attacctagg cgccattacc aaagagctat atcctgctgt ggctgataaa tttaatacta
      841 ctccaagcag ggtagagcgg gccatccgcc atgcaataga ggtagcttgg aatagaggta
      901 aaattgaaac tttaaataag atttttggtt atactgtcag tactgacagg ggcaagccta
      961 ccaatagtga gtttattgcc ttaatatctg acaaactaag gctagaattt tctgacttag
     1021 gtggaattag ctagttttaa actcatttat ttttaactaa taagtttata tgctataata
     1081 gttacaaata aagaaatttt ctttaaatta tgtttggagg ttaatattaa tggattatca
     1141 ggaaaaaact atgaaaactg ataagcttta tgagggcaaa attcttaacc taagaattga
     1201 cacagttgaa ttacctaata aaaagtattc taaaagagaa atagttgaac atgctggcag
     1261 tgtggccata atacctatga tttctgatga ttcattggta ttagtaaggc aatacagaaa
     1321 ggctgttgaa gactttttat tagaagttcc tgcagggaga ctagagttaa atgaagagcc
     1381 tagggagaca gccataaggg aattaaaaga ggaaacaggc ctaaacacaa aaaaactaga
     1441 atatatatcc gaattttatg cctctccagg atactgtaac gaaaagatat acttattttt
     1501 agcaaaggat ctatttgaag ataaagaatt tattcaagat agggatgaag aatatataga
     1561 gaaggaaatt tatactttcg atcaattact ttccatgata aaaaaagggg acattgtaga
     1621 ttg
//
LOCUS       DATGSI010000071         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_572182, whole genome shotgun sequence.
ACCESSION   DATGSI010000071 DATGSI010000000
VERSION     DATGSI010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572182"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..743)
                     /locus_tag="VK031_01095"
     CDS             complement(66..743)
                     /locus_tag="VK031_01095"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HLS52544.1"
                     /translation="MKKIFNNDWQELLAEEFEKEYYKKLRAYLIQEYRTKTIYPDMYD
                     IFNALHYTAYKDVKAVILGQDPYHGPNQAHGLAFSVQKQVRIPPSLRNIYKELHSDLG
                     LSIPNHGYLKKWADEGVLLLNTSLTVRAGQANSHKNIGWEIFTDNIISLLNKRKDPIV
                     FILWGNNAKSKERLIKGSHHHIIKSVHPSPLSASRGFFGSKPFSKTNNFLKSIGKGPI
                     DWQIENI"
     gene            complement(921..>1510)
                     /locus_tag="VK031_01100"
     CDS             complement(921..>1510)
                     /locus_tag="VK031_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52545.1"
                     /translation="GVSSLSIMGANLMAIFIYLIIYNYENFYKNFPLALSFSITRQDF
                     FKSILVKNLGISLTLGMIQGLLLTIDAPLVRSIGRVPIVEFVLFNTELDKAIYIIFAI
                     SIVFLTFLSLWSVVAALNYRFGYIIWIIFVAIQFLLERLNLAHIYNNIFQYIFNLFFN
                     RIDLGSFLQLFLIILISHGLTYIITINTDIQYKSA"
BASE COUNT          509 a          228 c          253 g          520 t
ORIGIN      
        1 cttctttcaa tttatatcgg caggatttaa ggttaaatta agagggcagg taaattttgc
       61 ttaaattata tattttctat ctgccagtct ataggccctt tacctatgga ttttagaaag
      121 ttattggttt tagaaaaggg cttagagccg aaaaatcctc tgctggcaga taaggggcta
      181 gggtggacag actttattat atggtggtga gagcccttta ttaacctttc tttacttttg
      241 gcattattcc cccaaagaat aaagactatt ggatcttttc gtttatttag taggctaatt
      301 atattgtcag taaatatttc ccagccaata tttttatggg agttagcctg gcctgccctt
      361 acagtaagag aggtatttaa caaaagcacc ccttcatcag cccatttttt caaatagcca
      421 tgattaggta tgctaaggcc taggtcactg tgaagctcct tataaatatt tcttaaagaa
      481 ggtggaattc tcacctgttt ttggacagaa aaggccaaac catgagcctg gttaggtccg
      541 tgataaggat cttgacctag tattactgcc tttacatcct tgtaggctgt ataatgtaag
      601 gcattaaaga tatcatacat atctgggtat atggtttttg ttctatattc ttggattaaa
      661 taagcccgta attttttata atattctttt tcaaattctt cagctaataa ttcttgccaa
      721 tcattgttaa atattttttt caatagtttc acccctaaat ctctttatcc attattatat
      781 atcctatctt aaaaaaggaa attttgaaaa tagttttatg tctaaacagc taagtttttc
      841 ctcgaaacac aatacctcaa ataattagtt ttaaaatata attttaggga taaagaaaaa
      901 gagattctaa ttagaatctc ttaggcagat ttatattgaa tatcagtatt tattgtaatt
      961 atataggtta gcccatggga gattaggata attaaaaata gctgtaggaa agaacctagg
     1021 tctatcctgt taaagaaaag gttaaatata tactggaaga tattattata tatgtgggct
     1081 aagtttaaac gttctagcaa gaattgaatg gccacaaaaa ttatccatat tatatatcca
     1141 aatctataat ttaaggctgc aactacactc catagggaaa gaaaggttaa aaatactatt
     1201 gagattgcaa atataatata aattgcctta tccaattctg tattaaatag gacaaattct
     1261 actataggaa ccctaccaat agatcttacc aagggagcat ctatggttaa taaaagtcct
     1321 tggatcatgc ctaaggttag ggatatgcct agatttttta ctagaatgga tttaaaaaaa
     1381 tcctgcctag taatactaaa ggatagagct aggggaaaat ttttatagaa gttttcataa
     1441 ttatatataa ttaaatatat aaaaatggcc attaaattgg cccccattat ggataaggaa
     1501 gatacccctc
//
LOCUS       DATGSI010000072         1075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_666330, whole genome shotgun sequence.
ACCESSION   DATGSI010000072 DATGSI010000000
VERSION     DATGSI010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666330"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VK031_01105"
     CDS             complement(<1..188)
                     /locus_tag="VK031_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52546.1"
                     /translation="MDKNFTKKEVIVVLIIVLTLVSLLTFKLLSSNLEKKTRASLKVE
                     EVEEEDLEEETEIIMVHI"
     gene            357..>1075
                     /locus_tag="VK031_01110"
     CDS             357..>1075
                     /locus_tag="VK031_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLS52547.1"
                     /translation="MKRYLILFIVFAVLLNTYSFAVADDLSLNGEGAILIDYDSGRIL
                     YEKNAHKKLYPASTTKMMTAILTLEHGKMDDMVTIDPEIIGMTEGSHIALDYDEVMSV
                     EDLLHALMIASANDAAAALGKYVAGSIDGFVKMMNDKARELGATNTNFTNPSGLHDDN
                     HYSTAYDLYLIARYAMENKSFRELVKKSSYTIGPTNKKSEPRLLHTTNRFLYGNDRIN
                     VDGKTIPIRFDGVAGIKTGTTG"
BASE COUNT          334 a          186 c          205 g          350 t
ORIGIN      
        1 cttatatgga ccatgattat ttcagtttct tcttccaggt cttcttcttc cacttcctcc
       61 acttttaggc tggccctggt tttcttttcc aaattacttg aaagtagttt aaaagttagt
      121 aatgatacca aggttaaaac aattataagt acaactataa cttccttttt tgtaaagttc
      181 ttatccatac ttatccccca ttctacaagc cttaaaattg taaaaaggtg tataaatatg
      241 actaatttta cccctatatt tattttactt aactcttggg aaaaataaaa ttcttttact
      301 taaatttttt aagattattt gctataatag gtatagcaga aaaggtggtg gattatttga
      361 agagatatct aatattattt attgtctttg cagtactatt aaatacatac tccttcgcag
      421 tagcagatga tttatcatta aatggagaag gggcaattct aattgactat gattctggta
      481 ggatccttta tgagaaaaat gctcataaga aattataccc tgccagtacc accaagatga
      541 tgacagctat tctcaccttg gaacatggca agatggatga tatggttact attgacccgg
      601 agatcatagg catgactgag ggcagccaca tagccctaga ctatgatgag gtaatgtctg
      661 tagaggacct gcttcatgcc ctaatgattg cttcagccaa tgatgctgct gcagcccttg
      721 gtaagtatgt ggcaggatct attgatggct ttgtaaagat gatgaatgac aaggctagag
      781 aattaggggc tacaaatact aattttacta atcccagtgg ccttcatgat gacaaccatt
      841 actctacagc ctatgactta tacttgattg ccaggtatgc catggagaat aaaagttttc
      901 gtgagctggt caagaaaagt agctacacca taggccctac taataaaaaa agtgagccta
      961 ggctgcttca tacaactaat aggtttttgt atggtaatga taggattaat gtggatggta
     1021 aaactattcc cattaggttt gatggagtgg ctggaatcaa gacaggtact acagg
//
LOCUS       DATGSI010000073         1034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_666354, whole genome shotgun sequence.
ACCESSION   DATGSI010000073 DATGSI010000000
VERSION     DATGSI010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666354"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..425
                     /locus_tag="VK031_01115"
     CDS             156..425
                     /locus_tag="VK031_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52548.1"
                     /translation="MNLDNIVERRIVDRGQHKMVLELSKDEYNKVYNEYSDHIARRIV
                     RDHLMNRDDDGRPLNVKMERPGGGNIIKIYADLSYLGNLHTSYDE"
     gene            517..>1034
                     /locus_tag="VK031_01120"
     CDS             517..>1034
                     /locus_tag="VK031_01120"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HLS52549.1"
                     /translation="MELGFDLKLEQTQKLIMTQELRQAIQLLQFNSFELNQYLKNEIE
                     ENPMLELESGLEEMEMVKELPNEREVDWKEFIEKYDDVSYKPQVDKNQEEYSYESFIS
                     YEPTLKEYLLNQLNLVKIDEKKYIIAEYIIQSIDENGYLKSTVEEIAYLLGASLAQVE
                     EALRLVQSFEPTG"
BASE COUNT          397 a          125 c          223 g          289 t
ORIGIN      
        1 agttcaagag cacctaaggc actgggagtg ccctatgata aatcggaaag atagcagtgt
       61 aagagagggg aagattcccc tctctttttt acataaattt attatttggt gaaaaaataa
      121 ttaataccaa attaaaaata aagggtgatg gttttatgaa tctggataat attgttgaaa
      181 gaagaatagt ggatagggga caacataaaa tggttcttga attgtcaaag gatgaatata
      241 ataaagtata taatgaatac agtgaccata ttgctagaag gatagtaagg gaccatctaa
      301 tgaataggga tgatgatggg aggcctttaa atgtaaagat ggaaaggcca ggagggggga
      361 atataataaa aatttatgct gatttaagtt atcttggaaa tcttcatact tcctatgatg
      421 aatagatggg tgaaaagatt tcttcatttg tccaagattg tcaattgtta aattattatg
      481 gtataattgg ttaaaaggac agggagtgat tgtaggatgg aacttggttt cgatttaaag
      541 ctggaacaga cccaaaaact cataatgacc caagaactta gacaagctat tcaattactc
      601 caatttaata gttttgaatt gaatcaatat ttaaaaaatg aaatagaaga aaaccctatg
      661 ctggaactgg agtctggctt agaagaaatg gaaatggtaa aggaattacc aaatgagagg
      721 gaagttgatt ggaaagaatt tatagaaaag tatgatgatg taagctataa acctcaggtg
      781 gataaaaatc aagaagaata ttcctatgag tctttcataa gctatgagcc caccctaaaa
      841 gaatatttat taaatcagtt aaatctagta aaaatagatg aaaaaaagta tataatagca
      901 gaatatatta tccagtctat tgacgaaaat ggctatctaa agtctacagt agaggaaatc
      961 gcttacctcc ttggtgcaag cctggcccag gtggaggagg ccttaagact agtacaaagc
     1021 tttgaaccta cagg
//
LOCUS       DATGSI010000074         1225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_334347, whole genome shotgun sequence.
ACCESSION   DATGSI010000074 DATGSI010000000
VERSION     DATGSI010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334347"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VK031_01125"
     CDS             complement(<1..726)
                     /locus_tag="VK031_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HLS52550.1"
                     /translation="MKTNIIEGAQLKRALANAVRCLEKNKEEVNSLNVFPVPDGDTGT
                     NMLLTIKSALKQGLEADENDTSKIAQAASQGSLMGARGNSGVILSQLFRGFANGLQGK
                     VNIDIETLAKGLKEASDTAYKAVMKPTEGTILTVARECGEYAMATYRRERDILEFLRK
                     IIDHGNETLNKTPDMLPVLKQAGVVDAGGKGFMYILIGAYNSLAGIDEHIEEREDTKS
                     QAVHRDVEEIETDDIKYGYCTEFM"
     gene            complement(743..1099)
                     /locus_tag="VK031_01130"
     CDS             complement(743..1099)
                     /locus_tag="VK031_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HLS52551.1"
                     /translation="MPAKTNTDLGTICIDNNVIATLAGISAMESYGIVGMASKNATDG
                     LFELLKWDHLTRGIKVYTKDDKLSIDLHVILQYGVKISVVAENIIERVKFNIEEFTGH
                     RVDNVNVYVQGIRVQK"
BASE COUNT          337 a          288 c          166 g          434 t
ORIGIN      
        1 cataaattca gtacagtaac catactttat atcatctgtt tctatctctt ccacatctct
       61 gtgtactgcc tgcgattttg tatcttccct ttcctctata tgctcatcta tacctgccag
      121 agagttatag gctcctatta atatatacat aaagcccttg cccccagcat ccaccacacc
      181 ggcctgcttt aaaacaggca acatgtccgg ggttttatta agggtttcat tcccatggtc
      241 tattattttt cttaagaatt ctaatatatc cctttctctt ctataggtgg ccatggcata
      301 ttctccacat tctctagcaa cggtcaggat agtcccctct gtcggcttca ttacagcctt
      361 ataagcagta tcggaggctt cctttagacc cttagccaaa gtttctatat caatatttac
      421 ctttccctga agaccattgg caaagcccct aaacaattgg gataggataa ctcctgaatt
      481 acctcttgcc cccattaagg aaccttggct agcggcttgt gcaattttcg atgtatcatt
      541 ttcatcagct tctaatcctt gctttagggc tgattttata gtcaatagca tattggttcc
      601 tgtgtcccca tcaggcacag gaaaaacatt taaggagtta acctcctctt tattcttttc
      661 aaggcaccgt acagcattag ctaaagccct cttaagctgg gcgccttcta ttatatttgt
      721 tttcaagtat agtacctcct aactactttt gaactctaat cccttgtaca tagacattta
      781 cattatcaac tctatgtcct gtgaattctt cgatattgaa tttgactctc tctattatat
      841 tctctgctac aacagatatt ttcactccat actgtaaaat cacatgtaaa tcaatagaca
      901 atttatcatc ttttgtataa acctttattc ccctagttaa gtgatcccat ttcaaaagtt
      961 caaaaagacc atctgtagca ttctttgaag ccatgcctac aattccatag ctttccatag
     1021 ctgatattcc tgctaaagtt gcaattacat tattatctat acaaatggtt ccaaggtcag
     1081 tattagtttt ggccggcata agcacactcc tccctgtata aaatatacca tatttaacat
     1141 tttacattat atgataaaat agttcaacta aatcctatct aaatataggc ctagtatatt
     1201 ttttagttgc attactatat attct
//
LOCUS       DATGSI010000075         1690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_53134, whole genome shotgun sequence.
ACCESSION   DATGSI010000075 DATGSI010000000
VERSION     DATGSI010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53134"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     repeat_region   2..229
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=69..98
                     /rpt_unit_seq="gttattagactacctataaggaattgaaac"
     gene            complement(310..>1690)
                     /locus_tag="VK031_01135"
     CDS             complement(310..>1690)
                     /locus_tag="VK031_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HLS52552.1"
                     /translation="NLTGIMISDNHITGHGNAGILSSHKASITVDKNLIENNGQGQGF
                     TRAGIILSFHGESKITNNTIINNGTDGIFAEESNSGLIIEGNLIENHTNSGVTLAWDE
                     HNVNILNNDIKNCGSGNLDEQGGIVIIQSAAELIQGNTIKGCSPYGLFWGWVTAIGPM
                     PDSILISENEISSNSQDGIYLFSQGPGGWIDPDNYPLKPNIENNYLLKNGGAGIYVSN
                     LYYSTPGNANPTVNNNKISNNVEWGIFNGTEEIIDATNNWWGDNSGPFNSLLNPSGKG
                     NPVSENVDFSPWITKGNGIDIVECRVDSVQIIYYQICPLEDETSLLKLTFRLIGQIYM
                     QVNDEIDTFPFEIIFNRRTKVDCFLSENSYPKLDITSSCQSYLINNKINVQINLCIKL
                     DICIKKNINPLNAMSYCPNIHLNGDSQKNLAGKGQITNKSCISVDLVLARYKITENLT
                     HHIPIEYLN"
BASE COUNT          543 a          287 c          265 g          595 t
ORIGIN      
        1 cgttgttaga ctacctataa ggaattgaaa ccaagacacg gtagagattg aaaacaggcc
       61 tagatattgt tattagacta cctataagga attgaaacac tgctttctca atagtataaa
      121 gtcccatatc tacggttatt agactaccta taaggaattg aaacattgga gaacttatcc
      181 actccttaat ttgtttaaag ttattagact acctataagg aattgaaaac cctcctaaat
      241 gatatgaact ctttaatccg aacacggaat ttgaggataa tatcaaaatt aggagggttt
      301 tatttataat caattcaagt attctatggg aatatggtga gttaggtttt cggtgatttt
      361 atatctagcc aatacaagat ctacacttat gcatgattta ttggttattt gacctttacc
      421 agcaaggttt ttttgagaat ctccatttag atgaatatta ggacagtagg acatagcgtt
      481 taatgggtta atattttttt taatacatat atctaatttg atacatagat ttatttgaac
      541 atttatttta ttatttatta gatatgattg acaacttgat gtaatgtcga gtttgggata
      601 actattttct gataaaaaac aatctacctt ggttcgtcta ttaaaaatta tttcaaaagg
      661 gaatgtatct atttcatcgt ttacttgcat atatatttgg ccaataagtc taaaggtcaa
      721 tttcagtaga cttgtttcat cctctaatgg acaaatttgg taatatatta tttgaacaga
      781 atcaactcta cattccacta tgtcaatacc gtttcccttt gttatccaag gggaaaaatc
      841 aacattttcg cttacaggat tacctttacc agaggggtta agaagactat taaaaggacc
      901 actattatct ccccaccagt tattggtggc atctataatt tcttctgtac cattaaagat
      961 accccactca acattattac ttattttgtt attgttaaca gtaggattgg cattgccagg
     1021 agtggagtag tataaattac ttacatagat accagcacca ccattcttta gtaaatagtt
     1081 attttcgata ttaggtttta gtgggtaatt atcaggatca atccaaccac caggtccttg
     1141 tgaaaataaa tatatgccat cttgagaatt agagcttatt tcattttcac taattagaat
     1201 tgaatcaggc ataggtccaa tagctgtgac ccagccccaa aacaagccat agggagaaca
     1261 gcctttaata gtatttccct gaataagctc agctgctgac tggataatta ctataccgcc
     1321 ttgttcatct agatttcccg agccacaatt ttttatatca ttattcaaaa tatttacatt
     1381 atgctcgtcc catgctagag taacgccaga atttgtatgg ttttctatta aattgccttc
     1441 tataattaaa ccagaattac tctcttctgc aaatatacca tctgtgccgt tattgattat
     1501 tgtattatta gtaattttac tttcaccatg aaaggaaaga attatcccgg ctcgcgtaaa
     1561 accttggcct tgcccattgt tttcaataag gtttttatct acagtaatag aagccttatg
     1621 actagataat atgcctgcgt ttccatgacc tgtaatatga ttatcagata tcataatacc
     1681 agttagatta
//
LOCUS       DATGSI010000076         6245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_525442, whole genome shotgun sequence.
ACCESSION   DATGSI010000076 DATGSI010000000
VERSION     DATGSI010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525442"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VK031_01140"
     CDS             complement(<1..643)
                     /locus_tag="VK031_01140"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HLS52553.1"
                     /translation="MKAIIGGTGLYETGEKVTKRIVDTDYGKVELDILDMGGEEVAFL
                     ARHGKEHSIPPHLVNYRANLMALKQLGVSHIFATAAVGSCNLNYAPGDLVSLKDFIDF
                     TKSRPMTFFEGGEKKVKHVDMSQPYSKELKEVFYKVAEELELEIKGEAVYVCTEGPRF
                     ETASEIKMYQSLGGDVVGMTNVPEVVLANELEIEYFCIGIITNWCTGFKGDIFF"
     gene            complement(692..919)
                     /locus_tag="VK031_01145"
     CDS             complement(692..919)
                     /locus_tag="VK031_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52554.1"
                     /translation="MVKTMEFKNQHLYLLDQRKLPLETSFFKCKTYQQVEFAIKDMVV
                     RIATEGVKVYNPAFDVTPSENISGIITEKEF"
     gene            1240..2754
                     /locus_tag="VK031_01150"
     CDS             1240..2754
                     /locus_tag="VK031_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52555.1"
                     /translation="MVILISTSACAQLANSPKIIQLEKSIKEYVLSKDESHDFLKDQV
                     YIEKIDKLLSEYTKRGLIASDHIAIGHLTETNIPELIVFREKDSSDASDEGSLEVYSF
                     DGEKYTLLDKVSMNFDNTNYQLEVGKLSKDQTGIYLNNQAGSQFGVTYGYILEDGKLK
                     SVLNPKKVNLISIDTDNEIKDITKDGILEFSVHLIDPESPDKDPETADKIKLWYRWDG
                     KDGAELISLEKEKGENSEMDKVSDQNILKEAEELLENEDQAFLDYLKENQQELTAADN
                     TKLLENYISLLEDRLYSEEGKVNKLIEQYESDAQEKKLTEQHGLTLERLNDSEYLRRQ
                     KVLSSHQELKEKIIKNLNLGYKLDKIGDKYRFVINYQLFIDDFGNNILNEYKDFLRIL
                     ELNSQESYLKTNKVNPPLEELAERIALAEDFRLTYPYSEKLGKINDFYQKCIDIFFSH
                     DMKASLAPKETTFSKVELLDRYEKIKEKYKHTYFADIIDRYIQENQPLQENEAS"
     gene            complement(2786..3064)
                     /locus_tag="VK031_01155"
     CDS             complement(2786..3064)
                     /locus_tag="VK031_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52556.1"
                     /translation="MRQNQELLEGNTGKIFLSFLLPSVGGMLGISLYVLGDTLLVGRG
                     LGSNGLTALNVSIPIMNIFAEGATLIYALVANELCRDALNHSLRTDTI"
     gene            3275..3847
                     /locus_tag="VK031_01160"
     CDS             3275..3847
                     /locus_tag="VK031_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HLS52557.1"
                     /translation="MIYRNATLEDIPAVARLQEQYHVSTIKPEDKADGFVTTLFTPEQ
                     FKRLIEEENGLAIACDGDQIVAYAMAASWDYWSEWPLFQYMIKDLPNIEYLGRTLSVE
                     NSYQYGPICIHKDYRGSEVLPNLFEFSRRQMAHKYPIMITFINHENPRSYRAHTKKLG
                     LDTIKNFEYNKNLYYTLGYDMSKKTPGSTI"
     gene            complement(4055..5002)
                     /locus_tag="VK031_01165"
     CDS             complement(4055..5002)
                     /locus_tag="VK031_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HLS52558.1"
                     /translation="MAFFRGQFANVVEWEEFRDDMIFWKWTNREIKRGSKLIIRPGQD
                     AIFMFNGKIEGIFTDEGEYDIESEIVPFLSTLKGFKFGFNTGMRAEVLFVNTKEFTVR
                     WGSKNAINIPTETLPGGLPIRANGTFNFKVGDYIALIDKVAGVRDSFLVEDVKLRITS
                     NLDQLLMKWITREGKDMFNLQANAFDISQGIKEDLDMISINDGISITGFNVISFNYPE
                     EIQEMINKNASHSMIGDMARYQQASMIDGISSGKVKGGGAASDMAGMMMGMKMAKEVM
                     KDVDINKEKEIKQRPNFCPNCGEKTGSGNFCSNCGEKLV"
     gene            complement(5027..5824)
                     /locus_tag="VK031_01170"
     CDS             complement(5027..5824)
                     /locus_tag="VK031_01170"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HLS52559.1"
                     /translation="MVRFNKYAKPLFIFLLILIILTNPSLAKSNKERIFDKANLLDKR
                     EKEDLEVLAEKYSQKRETDFIILTTSDPEGKNVVEYVEDFYDRMALGYDKRHGNVAIL
                     TIDMKNRELYLAGFYKGEEYLDKERLDKIRNKITPDLSKGNYFSAFKSFLEISYKYMG
                     IRPGVDPDNILFNLYFQIGAALIIAGISVGAMLYNSAGKAEVFAGTYQDHENSRIISS
                     RDRFIRKTVSRRKKPTNKPHGSGGMPRNTGGGRSSGGHSHSGSRGKF"
     gene            complement(5818..>6245)
                     /locus_tag="VK031_01175"
     CDS             complement(5818..>6245)
                     /locus_tag="VK031_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52560.1"
                     /translation="KLEPYDYGGIKDFNTPYLAGFIAEKYNYTDKELLPRARRKIENY
                     IDYFISSTISGYSTVQYRRKDINTRKKNAYYTLLPVWMVYYNYKGKTYIFAMNGQTGK
                     VVGQPPISRGKVAAWFTGIAISSFIMIKALAFGLGGGLW"
BASE COUNT         2017 a         1239 c          901 g         2088 t
ORIGIN      
        1 ggaaaaatat atctccctta aaacctgtac accaattggt aatgattcct atacaaaaat
       61 actctatctc caactcatta gccaagacca cctctggtac attagtcatt cctaccacat
      121 ccccgcctaa actttgatac atttttattt cactggctgt ttcgaatcta ggcccctctg
      181 tacaaacata aacagcctct cccttaattt ccagttctaa ttcttcagcc accttataga
      241 atacttcttt taactcttta gaatatggct gactcatatc cacatgtttt accttttttt
      301 ctccaccttc aaaaaaagtc ataggtctgg acttggtaaa atctataaag tccttaaggc
      361 ttaccagatc tccaggagca taatttagat tgcatgaacc tactgctgct gtggcaaaga
      421 tatggctaac ccctaactgc ttcaaggcca taaggtttgc cctataattt accaaatggg
      481 gtggtataga atgttcttta ccatgtctag ccaaaaaagc tacctcttct cctcccatgt
      541 ctaaaatatc taattcaacc tttccataat ctgtatctac tatccttttt gttacctttt
      601 cccctgtctc gtagaggcct gtacccccaa ttatggcttt catataaatc ctcctatctt
      661 aaatttttaa tactatcccc ataaggagcc ttcaaaactc cttttctgtt attataccac
      721 ttatattctc acttggtgta acatcaaagg ctggattata aacctttacc ccttcagttg
      781 ctatccttac caccatatcc ttaatagcaa actccacctg ctgataagtt ttgcacttaa
      841 aaaatgaggt ttctaaagga agttttcttt ggtccaatag atacaagtgc tgatttttaa
      901 actccattgt tttaaccata ctatttccac cctttcacaa aattacttgt taaaagcctt
      961 aagatacctt ggttattgat ttgaaaacta tcttatttct tacctgccta tatatcttat
     1021 tctatatgtt ttttaatttt ccttttttaa aagcaagtta aaaggaagcc tactggtttt
     1081 atatttgaac tttacttttc ttaacaaaat ctttttgtat ttataggaag cctttctgta
     1141 acttctttgt tacttaataa ttattaaata ggtatataat tagcttagaa agaggggtga
     1201 attcagatta ggactaagat ttatttttta ataattctgg tggtaattct gattagcaca
     1261 tcagcctgtg cccaattggc taattctccc aaaattattc agttagaaaa atcaatcaaa
     1321 gaatatgttc tgtctaaaga tgaaagccat gactttttaa aagatcaggt ttatatagaa
     1381 aaaatagata aattattatc cgagtataca aaaagaggtc ttatcgcatc agatcatatt
     1441 gccataggcc acttaacaga aaccaatatt cctgaactta ttgtattcag ggaaaaagac
     1501 agttctgatg ccagcgatga gggaagtttg gaagtatact cctttgatgg tgaaaaatac
     1561 actcttttag acaaggtttc catgaacttc gacaacacca actatcaatt agaagttgga
     1621 aagcttagca aggaccaaac aggtatctac ctaaataacc aggccggttc tcaatttgga
     1681 gtcacttatg gatatatatt agaagatgga aaattaaaaa gtgttttaaa tccgaaaaaa
     1741 gtaaacctaa tttccataga tactgacaat gagattaaag acattacaaa ggatggaatc
     1801 ttagagttta gtgtccattt aatagaccca gaatctcctg ataaggatcc tgaaactgct
     1861 gataaaatca agctttggta taggtgggat ggaaaagatg gggctgagct tatttccctg
     1921 gaaaaggaaa aaggggaaaa ttctgaaatg gataaggtct ctgatcagaa tatcctaaaa
     1981 gaggctgaag aacttttaga aaatgaggac caagctttcc ttgattattt aaaggaaaat
     2041 caacaggagc ttactgctgc tgacaataca aaacttctag agaactatat atctcttttg
     2101 gaagatagat tatattccga ggaaggcaag gttaataaat taattgagca atatgaatcg
     2161 gatgcacagg agaaaaaact tacagagcaa catgggctca ctttagaaag attaaatgat
     2221 tctgagtatt taagaaggca aaaagttcta agttcccacc aagagcttaa ggaaaaaata
     2281 attaagaact taaatttagg ttataaatta gataaaatag gcgataagta taggtttgta
     2341 ataaattatc aattattcat tgatgatttt ggtaacaata ttctaaatga atataaggat
     2401 tttttaagga ttttagaatt aaattcccaa gaatcttatt taaagacaaa taaggtaaat
     2461 cctcctttgg aggaacttgc agaaagaatt gccttagcag aagactttag gctaacttat
     2521 ccttattcag aaaaattagg taagatcaat gatttctacc aaaagtgtat agatatcttc
     2581 ttttctcatg atatgaaggc tagcttagct cccaaagaaa ctactttttc taaagtggaa
     2641 cttttagaca gatatgagaa aattaaagaa aaatataaac atacctattt tgcagatatt
     2701 atagacaggt atatacaaga aaaccagcct ttacaagaga atgaagcttc ataaggaaaa
     2761 cccaccttaa ggtgggtttc ttatattaaa tagtatctgt ccttaaacta tggtttaaag
     2821 catctctaca caattcattg gcaaccaggg catatattaa tgttgcacct tctgcaaaga
     2881 tattcattat aggtatagat acatttaagg ctgttagccc attgctaccc aaacctcttc
     2941 ctactaataa agtatcacca agtacataca gggaaatgcc cagcatacct cccacacttg
     3001 gcaataaaaa agataagaaa attttacctg tattaccttc tagcagttcc tgattttgtc
     3061 tcaattattt acccccttat ttttttgata caacacatta tatattaagc ctattggggc
     3121 tagaatgtca gatatataac ataatcttaa agcttaagcc ccttaaaact ggatggacag
     3181 cttctagaca ttacctaatc catataataa aatgtatgta tagcttccta ttatgtttag
     3241 catgggaaca aatttatatt tagggggaat taaattgatc tatagaaatg ctactttaga
     3301 agacatacca gccgtagcta ggctccagga acagtaccat gtatccacta ttaagcctga
     3361 agataaggca gacggatttg taactaccct gtttacacca gagcaattta aaaggttgat
     3421 agaagaagaa aatggcttgg ctatagcctg tgacggtgat cagattgtgg cttatgctat
     3481 ggcagcttct tgggactatt ggtcagagtg gcccttgttt caatacatga tcaaggattt
     3541 acccaatatt gaatatttag gtaggaccct ttcagtagaa aattcctatc aatatgggcc
     3601 tatctgtatc cataaagact atagaggtag tgaagtttta cctaatttat ttgaattttc
     3661 tagaagacaa atggctcata aataccctat tatgatcacc tttattaacc atgaaaatcc
     3721 cagatcttat agggctcata ctaaaaaatt aggattagat acaattaaaa actttgaata
     3781 caataaaaac ctatactata ccctaggata tgatatgagt aagaaaacac caggctccac
     3841 catataggat taaagatacc cccctaaatt tagtggggta tctttaaccg ttataatcta
     3901 aacagtagtt tattttaata tttatctttg ttcaagatct atccaccatc tacagttggc
     3961 ccttctattt taggaaacat ctcttatctc cattatctct tgaatcttta tattctactt
     4021 aaaacccaaa gcttataacc ttacactttc tatattacac taatttttct ccacagttac
     4081 tacaaaaatt ccctgaacca gttttttctc cacaattagg gcagaaatta ggtctttgtt
     4141 ttatctcctt ttccttattt atatcaacat ctttcattac ttccttggcc attttcatgc
     4201 ccatcatcat tcctgccata tctgaagcag cccctccacc ttttaccttg ccagaagata
     4261 tgccatctat catagaggcc tgctgatatc tagccatatc tccaatcatg ctatgggagg
     4321 catttttatt aatcatttcc tgtatttctt ctggataatt aaagcttatt acattaaatc
     4381 ctgtaatact tatgccatca tttatgctaa tcatatctaa gtcttcctta attccctggg
     4441 atatatcaaa ggcattggct tgaagattaa acatgtcctt gccttccctt gtaatccatt
     4501 tcattagtag ttggtcaagg tttgaggtaa ttcttaattt aacatcctcc actaaaaagc
     4561 tatctctcac ccctgcaacc ttatctatta aggctatata atcccctacc ttaaaattaa
     4621 aggttccatt ggctctaata ggcaaaccac caggtaaagt ttctgtagga atattgatgg
     4681 catttttact tccccaccta actgtaaact ccttagtatt tacaaataat acttctgccc
     4741 tcatcccagt attgaagcca aacttaaaac cttttaaagt agatagaaaa ggaacaattt
     4801 cagattcaat atcatattcc ccttcatctg taaaaattcc ttcaatcttt ccgttaaaca
     4861 taaaaattgc atcctggcct ggcctaataa ttaatttact gcctcttttt atttccctat
     4921 tagtccactt ccaaaaaatc atatcatccc taaattcttc ccattctact acattggcaa
     4981 attgacctct aaaaaaagcc atatctaatc cctccttata gtctagttaa aattttcccc
     5041 tactgccact gtgggaatgg cctccgctac ttctgcctcc accagtattt ctaggcatac
     5101 caccacttcc atggggctta ttagtcggtt tttttcgcct acttacagtt tttctgataa
     5161 atctatccct gctgcttatt attctgctat tttcatgatc ctggtaagta ccggcaaaaa
     5221 cttctgcttt tccagctgaa ttataaagca tggctccaac tgaaatccct gctattataa
     5281 gagccgctcc tatttgaaag tatagattga ataaaatatt atctgggtct acaccaggtc
     5341 ttattcccat atatttataa ctgatctcca gaaagctttt aaaggctgaa aaatagttac
     5401 ctttagataa atctggagtg attttgtttc taatcttatc caatctttcc ttgtctaaat
     5461 attcttctcc cttgtaaaaa cctgccaaat atagctccct atttttcata tctatggtta
     5521 agatggctac atttccatgc cttttgtcat aacctaaagc cattctatca taaaagtctt
     5581 ccacatattc tactacattc ttaccttcag gatcactggt cgtaagaatt ataaaatcag
     5641 tctctctttt ttggctatac ttttccgcca gtacttccaa atcttccttt tctcttttat
     5701 ccagtagatt ggctttatca aaaattcttt ctttattcga tttagccaga ctagggtttg
     5761 ttaatataat aaggataagc aaaaaaataa ataagggttt ggcatattta ttaaatctta
     5821 ccataggcca cctcccaacc caaaggccag ggcctttatc ataataaaac tagatattgc
     5881 aattccagta aaccaagccg ctacctttcc tctactaata ggtggttgac ctaccacctt
     5941 gccggtctgt ccgttcattg caaatatata ggtcttgcct ttgtaattat aataaaccat
     6001 ccagacaggc aacagggtat aataagcatt cttttttctg gtatttatat cctttcttct
     6061 atactggaca gtagaatacc ccgaaatcgt tgaactaata aaataatcta tataattttc
     6121 aatttttctt ctggctctag gtaagagttc cttatctgta taattatatt tttctgctat
     6181 aaagccagcc aaatagggtg tgttaaaatc ctttatacca ccgtaatcat aaggctccaa
     6241 cttat
//
LOCUS       DATGSI010000077         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_288002, whole genome shotgun sequence.
ACCESSION   DATGSI010000077 DATGSI010000000
VERSION     DATGSI010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288002"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VK031_01180"
     CDS             complement(<1..690)
                     /locus_tag="VK031_01180"
                     /inference="COORDINATES: protein motif:HMM:NF037741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52561.1"
                     /translation="MRETLYSLAEPIRKKIPRDAEVFPYMKLLLSVELMAVAYSQLQV
                     KVSLKEAWTLLTGYDAPILPENYSKKIIEKLRMLLPNFSAEGTWLEALEIYQGLDVTY
                     RLLEIDEEGYFTYKTPNLDPNRKELYEEILRKAIPYKESDKQFAGAGKFSYHKPSLDS
                     KKVMTGVIPQAWLKEAKRLPVYREKKEISFKLDYNWLDIAREMDEKLEKKHWEQTFKP
                     IEFRAIGEDKEF"
     gene            complement(687..>1037)
                     /locus_tag="VK031_01185"
     CDS             complement(687..>1037)
                     /locus_tag="VK031_01185"
                     /inference="COORDINATES: protein motif:HMM:NF037054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52562.1"
                     /translation="YTLLPGIAESKIAENLEKRGYKISMYPGIDEADILVEKDGNKLV
                     LDVKDYYSPFYFAKHISQQDTKGELEGVFFVIPQARLDIFPSYINQAKNYYAKPINLL
                     SENELYRRVGDLLG"
BASE COUNT          295 a          238 c          161 g          343 t
ORIGIN      
        1 aaattcctta tcctccccaa tagcgcggaa ttcaatcggt ttaaaggttt gctcccaatg
       61 ctttttttct agtttttcat ccatttcccg agcaatatct aaccagttgt aatcgagttt
      121 aaaactaatt tccttcttct ctcgatagac tggcaatctc ttagcttcct tcagccaagc
      181 ctggggaatc acacccgtca ttactttctt actgtcaaga gaaggtttgt gatagctaaa
      241 cttcccagct cccgcaaatt gcttatcact ttccttgtaa ggaattgcct ttcttaaaat
      301 ttcttcataa agctccttac ggttaggatc taagttcgga gttttatagg taaagtaacc
      361 ctcctcgtca atctcaagca aacgataagt tacatccagc ccttgatata tttctaaagc
      421 ctctagccaa gttccttctg ccgaaaagtt aggcaagagc attcgtagtt tttctatgat
      481 tttcttggaa tagttttctg gtaaaatagg cgcatcataa cccgttagta aggtccaggc
      541 ctcctttaaa gacactttta cctgcagctg actataggcc acagccatta actcaacaga
      601 aagtaaaagc ttcatataag gaaatacttc cgcatccctt ggaatctttt tccgaatggg
      661 ttctgctaaa ctatacaaag tctctctcat cctaataagt cacctaccct tcgatataat
      721 tcattttcag ataatagatt aattggcttc gcgtagtaat ttttggcctg attaatatag
      781 ctaggaaaga tatctaaacg agcctgggga ataacaaaga aaaccccttc tagctccccc
      841 tttgtatctt gttggctaat atgtttagca aagtaaaagg gactataata atccttcaca
      901 tcaagtacaa gcttgtttcc atccttttct actaaaatat cagcttcatc aatcccagga
      961 tacatggaaa ttttatatcc tctcttttct aagttctcag cgatcttact ctctgctatt
     1021 cctggaagta gagtata
//
LOCUS       DATGSI010000078         1368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_478695, whole genome shotgun sequence.
ACCESSION   DATGSI010000078 DATGSI010000000
VERSION     DATGSI010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478695"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VK031_01190"
     CDS             <1..1257
                     /locus_tag="VK031_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cna B-type domain-containing protein"
                     /protein_id="HLS52563.1"
                     /translation="TDEEGNEYIYTVGEKRVPENYRDDYTIDEVDGSLVITNTYISPK
                     TEVKVEKEWVGGPADKPPIEVQLYRDGEKYKEPVTLDGSEGWTYTWTDLDERDKNGNK
                     YRYSVDEITGPENYGKSIEVDEENQITTITNTYNSPVTDIRARKLWELVPKEKPTIQL
                     QLYRDGEVMEGEEYLVTLEDGQYEYTWKDMPVTDENGIYYQYSIDEVQVPEGYRKIIS
                     EDGLTITNKYDDPEEPTFIKDIEGATKKDIEDGESFTYNVSMDFPTDISGYESLLIAD
                     ILEEVLEIESVEVQLDGVRSRFLEKYLTVEGNQVYLLLYEEDGFDFSIVAGRTINMEI
                     KAKIKEGADLSPYLELGIPNTATYRLNDREIIVSSETVTVTPPEPEKPELPSTGTNRA
                     ISILLYIIGFIALILGFILFKRPRQE"
BASE COUNT          526 a          203 c          302 g          337 t
ORIGIN      
        1 acagatgaag aaggcaatga atatatttat acagttggcg aaaaaagagt accggaaaat
       61 tatagagatg actatacaat agatgaagta gatggaagcc tggtgatcac taacacctat
      121 ataagtccga agactgaggt gaaagtggaa aaagagtggg ttggagggcc agcagataaa
      181 ccgcctatag aggtacagct atatagggat ggggagaaat ataaggagcc tgtaacttta
      241 gatggttctg aaggctggac ttatacttgg acagatttag atgaaaggga taaaaatgga
      301 aataagtata ggtattcagt tgatgaaatt actgggcctg aaaattatgg taaatctata
      361 gaggtagatg aagaaaatca gattacaacc ataaccaaca cctataatag tccagtaaca
      421 gacataagag ctagaaaatt atgggagctg gtacctaagg aaaagccaac catccaacta
      481 cagctataca gggatggaga agtaatggag ggtgaagaat atctagtaac tctagaggat
      541 ggtcagtatg aatatacctg gaaggatatg ccggtaacag atgaaaatgg gatctattat
      601 cagtatagta tagatgaggt tcaggtacct gaaggctacc ggaagattat atcagaagac
      661 ggcttaacaa taacaaacaa atatgatgat cctgaggaac ctacctttat aaaggatata
      721 gaaggagcta ctaagaagga tatagaggat ggagaaagct tcacttacaa tgtaagcatg
      781 gatttcccaa ctgacatatc aggctatgag tctctattaa tagcagatat attggaagaa
      841 gtattagaaa tagaatcagt agaggttcaa ctagatgggg taagaagcag attccttgaa
      901 aaatatctaa cagtagaagg caatcaggtc tacctactcc tgtatgaaga agatggcttc
      961 gatttcagca tagttgcagg aaggaccatc aacatggaaa taaaggccaa aattaaggaa
     1021 ggggcagact taagtcctta tctagagcta ggaataccaa atacagccac atacaggcta
     1081 aatgataggg agataatagt ttcgtcagag acagttacag ttacaccacc agaaccagaa
     1141 aagccagagt tacctagtac aggaacaaat agagccatat cgatcctgct ttatataata
     1201 ggatttatag cattaatatt aggatttata ttatttaaaa gacctagaca agaataaaaa
     1261 taaaccctaa atcctttagg atttagggtt tttatctatt ctattaattt ttcagaggca
     1321 taatcatatt catctaccaa ttctcctaga gcaaaaaacc tttttcca
//
LOCUS       DATGSI010000079         1443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_196103, whole genome shotgun sequence.
ACCESSION   DATGSI010000079 DATGSI010000000
VERSION     DATGSI010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196103"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VK031_01195"
     CDS             complement(<1..234)
                     /locus_tag="VK031_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLS52564.1"
                     /translation="MDTVYYLIQNTLPVAVPLLLVALGGMFSEISGVINIALEGIMLF
                     GAFFAILFVYFVQDLGLNPQTILLMAMVVAAISG"
     gene            complement(234..1418)
                     /locus_tag="VK031_01200"
     CDS             complement(234..1418)
                     /locus_tag="VK031_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLS52565.1"
                     /translation="MENKKGLLKKINIDNLGTDGGLSSFTSAIIAILIGLIFGFLVML
                     VASPENALAGFQYIIAGGFSRFGNVLYFATPILMTGLAVGFAFRMGLFNIGASGQYTM
                     GMYFALYVGFMWNLPKGVHWIVAILAGLIGGMIWGFIPGIFKALLNVNEVITSIMFNY
                     IGMYLVDMWIRGSSVMYTATEARTKYLPKSVQIPALGMAGSNVNLGIIFAIVIGIILY
                     IVLEKTTFGYELKATGFNKFASEYAGMKGRKNIILTMVIAGGLAGLGGAFAILAPSAI
                     AGSSMTYEPINVIDPNGFNGIAVALLGNSNPIGIIFSAIFISHIQRGGSLTSLVGYKP
                     EIIDVVIAVIIYFSAFAMIINTSLANFITGRFKKDGEINGLEGDGSGSIDEEDNSKGV
                     DQ"
BASE COUNT          463 a          331 c          220 g          429 t
ORIGIN      
        1 gcctgaaatt gcagccacta ccatagccat caataggatg gtctgcggat ttaagcctaa
       61 atcctgtaca aaataaacaa acaagattgc aaagaaggca ccaaaaagca taattccctc
      121 tagggctata ttgattactc ctgaaatttc actaaacatt ccacctaggg ctactaaaag
      181 taaagggact gcaacaggta gggtgttttg aattaaataa taaaccgtat ccattattgg
      241 tccaccccct tactattgtc ttcctcatct atagatccag aaccatctcc ctctagccca
      301 tttatttctc catccttttt aaaccttcca gttataaaat tggctaaaga agtatttatt
      361 atcatggcaa aggcagagaa gtatatgatt actgcaatta ccacatcaat tatctccggc
      421 ttatatccta ctaaactggt tagggagcca cccctttgta tatgggatat aaatatggca
      481 gagaatatta ttcctatagg atttgaattt cctaaaagtg ccacagctat tccattaaat
      541 ccatttgggt ctataacatt tataggctca taggtcatgc tactgcctgc aatggcagat
      601 ggagccaata tagcaaaggc gcctcctaat cctgccagtc cacctgctat aaccatggtc
      661 aagattatat ttttcctccc cttcatacct gcatattcac tggcaaattt attaaagcct
      721 gtggccttta attcatatcc aaaagtggtt ttctccaaaa ctatataaag aattatccct
      781 attactatgg caaatatgat ccctaaattt acatttgaac ctgccatccc tagggcagga
      841 atttggaccg atttaggcaa atattttgtc cttgcctcgg tagctgtata cattacagaa
      901 gaacctctta tccacatatc taccaaatac atgcctatgt agttaaacat gatagatgta
      961 attacttcat ttacatttaa tagggccttg aaaatacctg gtataaagcc ccaaatcatt
     1021 ccacctatta ggcctgccag gattgccact atccaatgga ctcctttagg tagattccac
     1081 ataaaaccta catagagtgc aaagtacatc cccatagtat attggcctga tgctcctatg
     1141 ttaaatagac ccatcctaaa ggcaaagcct actgccagtc ctgtcattag tattggagta
     1201 gcaaaataca ggacatttcc aaacctacta aagccaccag caattatata ctggaagcca
     1261 gctaaagcat tctctggtga tgcaaccagc atgaccaaaa atccaaaaat taatccaatt
     1321 aaaattgcaa ttatggcaga agtgaatgag gataggccac catcggttcc taaattgtct
     1381 atgttaatct tttttaataa acccttttta ttttccatca tcaatctcct ttacctcatc
     1441 acg
//
LOCUS       DATGSI010000080         1878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_335121, whole genome shotgun sequence.
ACCESSION   DATGSI010000080 DATGSI010000000
VERSION     DATGSI010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335121"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VK031_01205"
     CDS             <1..231
                     /locus_tag="VK031_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HLS52566.1"
                     /translation="LGMKNLSKGVKIELGQQEVKIDVFITVEYGSNITQVGKKVQENV
                     KKTVETMTGLDVLEVNVNIQGVNIPKENKKEE"
     gene            388..798
                     /gene="nusB"
                     /locus_tag="VK031_01210"
     CDS             388..798
                     /gene="nusB"
                     /locus_tag="VK031_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HLS52567.1"
                     /translation="MGRKQAREGAMQLLFQMEVTKDYSHEALDLFLANHTFDEGEERY
                     INEAVSTIMENLEKIDEHINDNLEGWSIYRLAKVDLVVLRIAIYEILFRKDIPIEVSI
                     NEAIEIVKKFSSADSFKFVNGVLGGFVRSLDKNE"
     gene            791..1792
                     /locus_tag="VK031_01215"
     CDS             791..1792
                     /locus_tag="VK031_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52568.1"
                     /translation="MNKYFLGIDTSAYTTSLALIDNKENLLYDLRLPLTVKRGARGLR
                     QQEAVFQHLNNFPRLISKLSKSFPIKHIHTVAVSSKPRNLEESYMPVFIYGRNQGYIL
                     AQVLGCEFKEFSHQEGHIGAALINNKKVSEQEFFSFHISGGTTEFLQVKDGEDNLNIK
                     RIGGTLDISFGQLIDRLGVYLGLDFPCGKKLDELSRRGRLINLRVPISIKDKFWLNLS
                     GLENYFVGLVESGQYSNEDISLTLFHSICLILSQVINSLHQELSLNTIILTGGVMANT
                     YIRRNLGIYIGNASIKLIFPSVDKCTDNGVGIAYLAKRKIGHKWGWTSETFKGKPGK"
BASE COUNT          675 a          242 c          389 g          572 t
ORIGIN      
        1 ctaggaatga agaacctgtc taagggtgtt aagatagaat taggccaaca ggaagttaaa
       61 atcgatgtct tcattactgt tgaatatgga tctaatatta ctcaagtagg gaaaaaggta
      121 caggaaaatg ttaaaaagac tgttgaaaca atgacaggtc tagatgtatt ggaggtcaat
      181 gtaaacattc agggtgtaaa tatcccaaag gaaaataaaa aagaagaata aatacatttt
      241 taccctctgg aagcagaggg taattttaat agtccattga aatttgattc ttattgatta
      301 aatacttttt tagtgagata atattaaaag ctaatatgga aaatccttgt tttacatatt
      361 aatccaaagg atttaggagg aaaaaagatg ggtagaaaac aagctagaga aggtgcaatg
      421 caactactgt ttcagatgga agtcacaaaa gactattccc atgaagcatt ggatttgttt
      481 ttagctaacc atacctttga tgaaggtgaa gaaaggtata taaatgaagc agtgtcaaca
      541 attatggaaa atctagaaaa aattgacgaa catataaatg ataacttgga gggctggtct
      601 atttataggt tagccaaggt ggatttggta gtccttagga ttgccatata tgaaatatta
      661 tttagaaagg acattccaat tgaggtatcg ataaatgagg ccatagaaat tgttaaaaag
      721 tttagtagtg ccgactcctt caaatttgtc aacggtgtat taggtgggtt tgttaggagc
      781 ttagataaga atgaataaat attttctagg tatagatacc agtgcctata ccacgtcttt
      841 agccttaata gataacaagg aaaatttatt atatgactta agacttccct taacagttaa
      901 aaggggtgcc aggggcttga ggcaacagga ggctgtattc caacatctaa acaattttcc
      961 aaggcttata agtaagctat ctaaaagttt tccaataaaa catatccata cagttgcagt
     1021 aagttctaaa cctaggaatc ttgaagagtc ctacatgcca gtttttatat atggaagaaa
     1081 ccaaggatat atattggcac aggtattagg ctgtgaattt aaggagtttt cccaccagga
     1141 aggacatata ggggcagcct tgattaacaa caaaaaagta agcgaacaag aatttttctc
     1201 tttccatatt tctggaggga caactgaatt tcttcaggtg aaagacgggg aggataattt
     1261 aaatattaaa aggataggtg ggaccttaga tataagtttt ggtcaactaa tagatcggtt
     1321 aggggtatat ctaggtttag actttccctg cggtaaaaag cttgatgaat taagtagaag
     1381 gggccggctt ataaatttaa gggttcctat ttccataaag gataagtttt ggcttaattt
     1441 atctggactt gaaaattatt ttgtaggact agtagaatct ggacaatatt ccaatgaaga
     1501 tataagctta accctttttc atagtatatg cctaatcctt tctcaagtta tcaatagtct
     1561 tcatcaagaa ttaagcttaa ataccataat ccttacaggt ggagttatgg ctaatactta
     1621 tattagaaga aatttgggta tctatattgg taatgcatct attaaactta tttttcccag
     1681 tgttgataaa tgtacggaca atggagtggg gatagcatat ttagctaaaa ggaaaatagg
     1741 tcataaatgg gggtggacta gtgaaacctt taaaggtaag ccaggtaaat agttatataa
     1801 aaaggttatt gtcagaggat atgattttat ctgatattca aattgaaggc gaagtatcta
     1861 attacaagaa acattata
//
LOCUS       DATGSI010000081         1747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_573213, whole genome shotgun sequence.
ACCESSION   DATGSI010000081 DATGSI010000000
VERSION     DATGSI010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573213"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1747
                     /locus_tag="VK031_01220"
     CDS             <1..>1747
                     /locus_tag="VK031_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLS52569.1"
                     /translation="RGLAQNEVELRLNKYGLNELKEEKRTTFASKLISQFSDFLVLIL
                     IGAAIISMAVGELKDAIVILAIVIVNAFLGIYQEGKAEKALDALKKMASSNAKVIRDG
                     KAVVIPANTLVPGDIVLLDAGDIIPADLRLIESSNLKIEEASLTGESVPIEKRANEVY
                     KEEVSLGDRHNMAYMSTVVTYGRGRGVVVGTGHDSEIGKIATMIQSFEDETTPLQRKL
                     NQLGKVLGITTIVICIIVFGIGLLQGREVLEMLMVAISLAVAAIPEGLPAIVTIVLAI
                     GMNRMVKRHAIVKKLLAVETLGCTTVICSDKTGTLTKNEMTVTKIFVDDKVVDITGTG
                     YEPRGKFKIDEKEISVNSLVNLKTLLSIGILTNDAKLEKTNDLYRIIGDPTEGALVTL
                     AGKANIRSQDINRAFPRVEEIPFDSDRKMMTTFHKNFIQEKLVSFTKGAPDIIISKCD
                     FIREGDNILPFTDDLKKRVLEVNSKLAMDALRVLAFAYKEYDNLPDNISSDNIESNMV
                     FVGLVGMIDPPRPEAKEAIRLCEQAGINTIMITGDYKETAFAIAKDLGMAETEDQSMM
                     GAELNNISDEELKEVV"
BASE COUNT          630 a          231 c          376 g          510 t
ORIGIN      
        1 aagggggcta gctcaaaatg aagttgaatt aagactaaac aaatatggac taaacgagct
       61 taaggaggaa aaaagaacca catttgcatc taaattaatt tcccagtttt ctgatttttt
      121 agttcttata ttaattggag cagcaattat ttctatggct gtaggtgagt taaaggatgc
      181 tattgttata ttggcaatag taatagtaaa tgccttttta ggaatctatc aagaaggaaa
      241 ggcagaaaaa gcattagatg cattaaaaaa aatggcttct tccaatgcga aggtcataag
      301 ggatggcaaa gctgtggtaa tacctgccaa tacattggtc cccggcgata ttgtgctatt
      361 agatgctgga gatataatcc ctgcagattt aagacttata gaaagctcta atcttaagat
      421 tgaagaagct tctctaacag gggaatctgt cccaattgaa aaaagggcta atgaagttta
      481 taaggaagaa gtttcattag gtgatagaca taatatggcc tatatgagta ctgttgttac
      541 ctatggtaga ggtaggggag ttgtagtagg tacaggtcat gatagcgaaa tagggaaaat
      601 cgctactatg atacagagtt ttgaagatga aaccaccccg ttacagagaa aattaaatca
      661 gctggggaaa gtgctaggca ttacaactat tgtaatttgt ataattgtat ttggaatagg
      721 tttgttgcag ggtagggaag tattagagat gcttatggta gctattagtt tagcagtagc
      781 tgcaattcca gaaggacttc ctgctatcgt cactattgta ttggcaatag gtatgaatcg
      841 aatggtaaaa cgtcacgcca tagtaaaaaa acttctggca gtagaaactt taggttgtac
      901 caccgtaata tgttctgata aaactggtac actaacaaaa aatgaaatga cagttacaaa
      961 gatatttgtt gacgataaag tagttgacat tactggaact ggctatgagc caaggggtaa
     1021 atttaaaata gatgaaaaag aaatttcggt aaattccctt gtaaatttaa aaactttgct
     1081 atctataggg atattaacca atgatgcaaa gttagaaaaa actaatgatt tgtatagaat
     1141 tattggtgat ccaactgaag gtgctttagt tactttagcc ggtaaagcta atatacgaag
     1201 tcaggatata aacagggcat ttcctagagt agaggaaatt ccatttgatt ctgatagaaa
     1261 gatgatgaca acatttcata aaaactttat acaagaaaaa cttgtatcat ttaccaaggg
     1321 tgcacctgat ataatcataa gtaaatgtga ttttattagg gaaggggata atatcctacc
     1381 atttactgac gatttaaaga agagggtttt ggaagttaat agtaaacttg ctatggatgc
     1441 cctaagggta ttggcctttg catacaaaga atatgacaat ctacctgata atatatcttc
     1501 cgacaatatc gaatctaata tggtttttgt aggtcttgtt ggaatgatag atccaccaag
     1561 accggaagca aaagaagcta taagactttg tgaacaagca ggtataaata ctataatgat
     1621 aactggtgat tataaagaga ctgctttcgc aatagctaaa gatttgggaa tggcagaaac
     1681 tgaagatcaa tcaatgatgg gtgcagaatt aaataatata tcagatgaag aactaaaaga
     1741 agtggtt
//
LOCUS       DATGSI010000082         1084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_196587, whole genome shotgun sequence.
ACCESSION   DATGSI010000082 DATGSI010000000
VERSION     DATGSI010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196587"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VK031_01225"
     CDS             complement(<1..314)
                     /locus_tag="VK031_01225"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583229.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HLS52570.1"
                     /translation="MRDYDKLIFELSKPGRKAYRLPPLDVEDRDIKEYIPDKFLNEEE
                     LDLPEVSEVDVVRHFTNLSDKNYGLDTGFYPLGSCTMKYNPKINEDMARLDGFTKLHP
                     LQ"
     gene            complement(316..>1084)
                     /locus_tag="VK031_01230"
     CDS             complement(316..>1084)
                     /locus_tag="VK031_01230"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583228.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HLS52571.1"
                     /translation="DLEALEELMGEDTGAVIVQSPNFFGIIEDLELISSMVKKFRRTS
                     LIASVDPISLGILKRPGDLGVDIVVGEGQALGMPLNFGGPYLGFIATTKKYMRKLPGR
                     IVGQTKDMDGKRSFVLTLSAREQHIRREKATSNICSNQGLNTLAATIYMVTLGKEGLR
                     EVALQSTKKAHYAFKEITKTGKYRPLFNKPFFKEFAIKSDISSDKIINDLRKENIIGG
                     YDLGRDYPEYENSILYAVTEKRTKDEIDKLSSLLEEI"
BASE COUNT          292 a          231 c          169 g          392 t
ORIGIN      
        1 ctttgcagag gatgtagctt ggtaaatcca tctaacctgg ccatatcttc attaatctta
       61 ggattatatt tcatggtaca ggagcctaaa ggataaaatc ctgtatctag gccataattt
      121 ttatcagaaa gattggtaaa atgcctaacc acatcaacct cacttacctc aggtagatcc
      181 aattcttcct catttaagaa tttgtctggt atatattctt ttatatccct atcttcaaca
      241 tcaagaggcg gcagtctata tgcttttcgt cctggttttg acagttcaaa aataagttta
      301 tcataatctc tcatattaga tctcctccaa tagggaacta agcttatcta tttcatcctt
      361 agtccttttc tctgtaacag catacagaat actattttca tattcgggat agtctcttcc
      421 taaatcatat ccgcctataa tattttcctt tcttaagtca tttattattt tatctgaact
      481 gatatcagat tttattgcaa actctttaaa aaagggttta ttaaataagg gcctatattt
      541 gcctgttttt gtaatttctt tgaaggcata atgggctttt ttggtgcttt gcagggccac
      601 ctctcttaaa ccctccttac ctagggtcac catatagatg gttgctgcga gggtatttaa
      661 cccttggttg gagcatatgt ttgaagtagc cttttccctt ctaatatgtt gttctctagc
      721 acttaaggtt agaacgaaag atctttttcc atccatatcc ttggtttgac ctactattct
      781 acctggaagc tttctcatat acttctttgt ggtggctata aatcctaaat agggtccacc
      841 aaagtttaag ggcataccta gtgcttgccc ttcacctact actatatcca ctcccaggtc
      901 tcctggtctt tttaagattc ctaaagatat aggatcaact gaagctatca gggaggtcct
      961 tctaaatttt tttaccatac tggagataag ctccaaatct tcaataatgc cgaaaaagtt
     1021 tggactttgt acaattactg ctccagtatc ttctcccatt agttcttcca aagcctccag
     1081 gtca
//
LOCUS       DATGSI010000083         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_335567, whole genome shotgun sequence.
ACCESSION   DATGSI010000083 DATGSI010000000
VERSION     DATGSI010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335567"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VK031_01235"
     CDS             complement(<1..402)
                     /locus_tag="VK031_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HLS52572.1"
                     /translation="MYQALYRRYRPKSFNELLGQDHITRILKNQIKKQNIGHAYLFSG
                     TRGTGKTSAAKIFSRAVNCLSPIDGNPCNKCENCQEILDNNTVDVIEMDAASNNSVDD
                     IRDLRDKVIYAPAKIKYKVYIVDEVHMLSKGA"
     gene            537..627
                     /locus_tag="VK031_01240"
     tRNA            537..627
                     /locus_tag="VK031_01240"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:572..574,aa:Ser,seq:gga)
     gene            640..906
                     /gene="ffs"
                     /locus_tag="VK031_01245"
     ncRNA           640..906
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VK031_01245"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(958..>1597)
                     /locus_tag="VK031_01250"
     CDS             complement(958..>1597)
                     /locus_tag="VK031_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HLS52573.1"
                     /translation="YTGSGKEGFSDFFNMFFGQGAQGFNLNDLFSGAKSAGFRSRSKP
                     LESELNLTIREAYEGVTKDISLNIDGERKNIRVKIPEGILPGKRLRVRGKKWGINRDI
                     LFKINFIEDDLFKIEGLDITSTINILPWEAALGSKVVINTLEGKIRLTIPKGITGGSK
                     IRIPNKGYVDMDKKRGDLYVKLNIVNPPNLSKEEIKMYEELSRLSTYNPRRE"
BASE COUNT          439 a          341 c          272 g          545 t
ORIGIN      
        1 ggccccctta gacaacatat ggacctcatc tactatataa accttatatt taatctttgc
       61 aggagcataa attaccttat ccctcaagtc cctgatatca tctacactgt tattgctggc
      121 tgcatccatt tctattacat ctacagtgtt gttatctaat atttcttggc agttttcaca
      181 cttattacat ggattaccat ctattggcga aagacagttt actgcccttg aaaatatctt
      241 tgcagcagaa gtttttccgg tgcccctggt tcctgaaaac aggtaggcat ggcctatgtt
      301 ctgcttctta atctggtttt ttaaaatcct tgttatatga tcttgcccca acaattcatt
      361 aaaacttttt ggcctgtatc ttctatacaa agcttgatac attgattttc acctacattt
      421 atagtattat ttatattata acagatatat ataagtttca caccaataaa ttaaaaaagg
      481 attaatagtt gacataattg tcattttagg ttataatata cttctgtaat atgtatggag
      541 aggtggccga gttggctgaa ggcgctcgcc tggaaagcgg gtaaacgtgt aagcgtttcg
      601 agggttcaaa tccctctctc tccgccatta taaattttgt cgtgctagat ggggaggtag
      661 cggtgcccta taacctgcaa tccgctatag caggattgaa ttcccagtta aggcacttct
      721 gttgtaaagt ctgccccaag caagtggcag tgacgattgg gtcctgcgca acggaaactc
      781 atgaaccccg tcaggtccgg gaggaagcag cggtaagtga accttttcgt gtgccgtagg
      841 ggtgcctggt ccgagttaac tacttgggta acgtttatga tagaattgtc gattctgggt
      901 gcacgactac atagtaaaag ctgtggcata agccacagct tttatatttt tttgtgccta
      961 ttcccttcta gggttataag ttgataaccg gcttaattct tcgtacatct ttatctcttc
     1021 tttgcttaaa ttaggaggat ttactatgtt taacttaaca tataggtctc ctctcttttt
     1081 atccatatct acatagccct tattaggaat ccttatttta ctgccacctg taattccttt
     1141 aggtatagtt aacctgattt ttccttccaa ggtatttata actaccttag agcctagggc
     1201 agcctcccaa ggcaaaatat ttattgtaga ggttatatct agcccttcaa ttttaaaaag
     1261 atcatcctca ataaaattaa tcttaaataa aatatcccta tttatccccc actttttgcc
     1321 ccttaccctt aatcttttgc caggtaaaat tccttcaggt atcttgaccc ttatattttt
     1381 tctttcacca tctatattaa gggaaatgtc cttggtaact ccttcataag cttccctaat
     1441 agtaaggtta agctctgact ccaagggctt acttctagac ctaaaacctg ctgatttagc
     1501 accggaaaat aaatcattta aattaaaacc ctgtgctcct tggccaaaaa acatgttaaa
     1561 aaagtcacta aagccctctt tcccagaacc tgtatat
//
LOCUS       DATGSI010000084         1821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_288274, whole genome shotgun sequence.
ACCESSION   DATGSI010000084 DATGSI010000000
VERSION     DATGSI010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288274"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VK031_01255"
     CDS             <1..551
                     /locus_tag="VK031_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HLS52574.1"
                     /translation="GKIKNVDATFTKLVSGNIRELDKGQAGGSMTELGTYPLLAIVKL
                     LGISPEEINYYSYFDKKREIDLFTKFNLVYKDAIATAKVGLGVKSEGDLIISGTKGYI
                     YVPSPWWKTEYFEARYEDFNDNKKYYYKFDGEGLRYELAEFVAMINNDRNESYKLTAK
                     ESMAIINVIETFLKRENITSLA"
     gene            570..1559
                     /locus_tag="VK031_01260"
     CDS             570..1559
                     /locus_tag="VK031_01260"
                     /inference="COORDINATES: protein motif:HMM:NF022822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stealth CR1 domain-containing protein"
                     /protein_id="HLS52575.1"
                     /translation="MYKAIDFVIPWVDGNDKDWQKEKAKYEGTNSGDNRDIRYRDYEN
                     LHFWFRGVEKYAPWVNKIHLITWGHLPAWLNVKHPKLNIVRHKDYIPKEYLPTFNSHV
                     IEIHLHRIEGLSEQFVYFNDDIFITRPLVREDFFENGYPKDIAALDVSIRADEIHGSA
                     VANCTYAINRYFDKNKTIKENFSKWFNPSYGKYLIKTLMLSSWKNFTGFQTTHLATSY
                     LKSTFEKVWEREETNLKATSQHKFRDKRDLMQFIFKFWQLAEGNFVPGKLLGKNYDLG
                     KELDSALHAIKNQHHKLICLNDNDGIKDFEAVKERVNKSFQKVFPKKSEFEIQ"
     gene            1586..>1821
                     /locus_tag="VK031_01265"
     CDS             1586..>1821
                     /locus_tag="VK031_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52576.1"
                     /translation="MKEETNLLNRKRLDVISILLLIIGWTVGLSLKDINAPIAATVLY
                     VSYKLDNINLLYSALFTLFLFINSIYLEKTGLMVG"
BASE COUNT          698 a          258 c          356 g          509 t
ORIGIN      
        1 taggaaaaat aaaaaacgta gacgccacct ttacaaagct ggtaagtggc aatataaggg
       61 aactagataa agggcaagca ggtggaagta tgacagaatt aggcacttat ccattgctgg
      121 ccatagttaa attgctaggc ataagtcctg aagagattaa ttattactca tatttcgata
      181 aaaaaagaga aatagatcta tttaccaaat tcaatctagt ttacaaagat gcaatagcca
      241 cagctaaggt aggactagga gtcaaatcag aaggggatct aatcatatct ggtactaaag
      301 gatacatcta tgttccatca ccctggtgga agactgaata ctttgaagcc aggtatgagg
      361 actttaacga taataagaag tattactaca aattcgatgg tgaaggttta agatatgaat
      421 tggctgaatt tgtggccatg attaacaatg atagaaatga aagttataaa ttaacggcca
      481 aggaatccat ggctataatt aatgtaattg aaaccttcct aaaaagggaa aacataacaa
      541 gcctagccta aaacatggag atgatttgta tgtataaagc tatagacttt gtaattcctt
      601 gggtagatgg aaatgacaag gactggcaaa aagaaaaggc aaaatacgag ggaacaaatt
      661 caggagataa tagagatata aggtataggg attatgaaaa tttacatttt tggtttagag
      721 gagtagaaaa gtatgccccc tgggtaaata agattcatct tattacctgg gggcatttgc
      781 cagcctggtt aaatgtaaaa caccctaaac taaatatagt tagacacaag gactatatac
      841 caaaagagta tttacctacc tttaactccc atgtaataga gattcattta cataggatag
      901 agggattatc agagcaattt gtatacttta acgatgatat atttattact agaccattag
      961 tcagggagga cttctttgaa aatggatatc caaaagacat agcagcctta gatgtatcca
     1021 ttagagcaga tgagatccat ggaagtgcag tggccaattg tacctatgca atcaatcgat
     1081 attttgataa gaacaaaaca ataaaggaga acttttccaa gtggtttaat ccttcttatg
     1141 ggaaatactt gataaaaaca ctgatgttaa gttcctggaa gaattttact ggatttcaaa
     1201 ctactcatct agcaacttct tatttaaaat caaccttcga aaaggtttgg gaaagagagg
     1261 agacaaatct taaagcaact tcccaacaca aatttagaga taaaagagac ttaatgcagt
     1321 ttatatttaa attttggcag ctggcagagg gaaactttgt gccagggaaa ttacttggga
     1381 aaaactatga tttgggaaaa gaactagact cagccctaca tgctattaag aatcagcatc
     1441 ataaactgat ttgcctaaac gataatgatg gaataaagga ttttgaagct gtaaaagaga
     1501 gagtaaataa aagtttccag aaggtatttc caaaaaaatc agaatttgaa atccaataga
     1561 taactagatc ttggaggtat aacaagtgaa agaggagaca aaccttttaa ataggaaaag
     1621 attagatgta atatctatac ttttattaat aataggatgg acagtaggac tcagtctaaa
     1681 agatataaat gcaccaatag cagctacagt tttgtatgtg agttataaac tggacaatat
     1741 aaacttactt tattctgctt tatttacctt atttttattt ataaactcca tatatcttga
     1801 aaaaacaggc ttgatggtag g
//
LOCUS       DATGSI010000085         1374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_620556, whole genome shotgun sequence.
ACCESSION   DATGSI010000085 DATGSI010000000
VERSION     DATGSI010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620556"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1019)
                     /locus_tag="VK031_01270"
     CDS             complement(60..1019)
                     /locus_tag="VK031_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLS52577.1"
                     /translation="MNTKDFQEIVNRFTTATDSQGNKLNMHSLLISLGDNIFLHRFNN
                     RQGYSDVRSISKTVLALVTGIVRDLSFKGKYSAFNENTYIYPIIKDVVNLKNPDNLPF
                     LEKIQVKHLLTHTMGYDKVLLMRGDIVDMDPFSYLDYIINEPIVYEPGKHYLYSNAGF
                     YTLSAVLQEFLQQDLISFIDEHLFGPLNIKEYKWEKYGHYLAGATRLWLLPEDLLKIG
                     QVLMNKGKYQGKEIVKADWIEKMLEPVARTKDVDTPDALFRRYSYAHGLWLAKENIFF
                     GHGTDGQTLVVVPEKKAILITLAEQKDVVKLEKIIDHIIREKI"
BASE COUNT          455 a          226 c          220 g          473 t
ORIGIN      
        1 cacatactcc cgccaagaaa aaacaagcca tgaaaatggc ttgtttttaa tgtttttgac
       61 tatatttttt ctctgataat atgatcaata attttctcaa gttttaccac atctttttgt
      121 tctgctaggg taataagaat agcctttttt tcaggaacca caaccagggt ctgcccatca
      181 gttccatggc caaagaatat attttcttta gccaaccaga gtccatgggc ataggaatat
      241 cttctaaata atgcatccgg tgtatctaca tccttagtcc tggcaacagg ctctaacatt
      301 ttttcgatcc agtcagcttt tactatttcc ttgccttgat atttaccctt attcattaat
      361 acttgcccaa tctttaacaa atcttctggt agtagccaaa gcctagttgc tcctgctaaa
      421 tagtggccat atttctccca cttatattcc ttaatattca atgggccaaa caaatgttca
      481 tctataaaag agattagatc ttgctgtaaa aattcttgta gaactgcaga taaggtataa
      541 aatcctgcat tagaataaag atagtgtttt ccaggctcat atactatagg ctcatttatt
      601 atatagtcaa gataggaaaa aggatccata tctactatat ctcctcgcat aagaagaacc
      661 ttatcatatc ccatggtgtg ggttaatagg tgtttgacct ggattttttc taaaaatggt
      721 agattatcag gattttttaa attaaccaca tccttaataa tagggtaaat ataagtattt
      781 tcattgaagg ctgaatattt ccccttgaaa gataaatccc ttactatacc agtaactagg
      841 gcaagaaccg ttttacttat acttcttaca tctgaataac cttgtctgtt attaaagcga
      901 tgtaaaaaga tattgtcccc tagacttata agcagggaat gcatatttaa tttattgccc
      961 tgggaatctg ttgcggtagt aaacctattt acaatttctt gaaaatcttt tgtgttcatt
     1021 atgacctcct gttgcattta gtttaatatt attatatcaa gactgtaatg attataactt
     1081 catagtatca gaaggtattt tatcgtcaag gaagataatc aatatgatta aatattgcta
     1141 aatcaattaa atatgttgga caactataaa taccctttaa acagtcttga ggtttgtaaa
     1201 acaatgtgtt acactgatgt aacaaaaggg ggcgagtata tgattacaat tagaattaga
     1261 ttggattaag aaagggaaaa aattattcag agataatgtg aagtaaacag tacttattta
     1321 tcaattttag tggaagaagt atttgagaag aagaataaag ctgggataga ttta
//
LOCUS       DATGSI010000086         1353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_526197, whole genome shotgun sequence.
ACCESSION   DATGSI010000086 DATGSI010000000
VERSION     DATGSI010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526197"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VK031_01275"
     CDS             <1..281
                     /locus_tag="VK031_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HLS52578.1"
                     /translation="TASATAGASVGGNIFGKAILGVGVKPLQASQMIYTGTLWFDSLP
                     HGSFFHVSAGSVFMEIKDRLKILPYELLIGIIMTIASVIAYGVLGINF"
     gene            406..1314
                     /locus_tag="VK031_01280"
     CDS             406..1314
                     /locus_tag="VK031_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HLS52579.1"
                     /translation="MNYEIITPVVTAFDRRGRIDYKANEEIIDFLIDGGVNGILVLGS
                     SGEFTELAFQEKKDFFDFYSEYVDGRLDLYAGTGSMKLKETVELSNYVSKRDYKGSFI
                     IGPYYYGQDQEKLLTYYNKLAKSIDGNIYIYNFPDRTGHSLSHDTIKQLADMNSNIKG
                     LKDSVTEPGHTNLCILATKENNFDVYSGFDDHYLYNIAAGGKGCISALSNIAPELWSS
                     LVKSSNEGDFENTIRLQSLVHELMPLYNMDSNFSYLFKRLMIYRGLNFEPKSIFPFDQ
                     LDEEVFVKAKRILDTVLAKFREIDYK"
BASE COUNT          490 a          178 c          263 g          422 t
ORIGIN      
        1 ctaccgcttc agcgacagca ggtgcctcag taggaggaaa tatttttggg aaagcaattt
       61 taggtgtagg cgtaaagcct ttacaagcct ctcaaatgat atatacaggg accttgtggt
      121 ttgatagttt accccatgga agctttttcc atgtaagtgc cggatcagtt tttatggaaa
      181 taaaagatcg tttaaaaata ttgccgtatg aattattgat aggaattatc atgactatag
      241 catccgtaat agcatatggt gttttaggta taaatttcta acaaaaattt aataatggta
      301 ttttcctcaa aatctagtcc tttgggatta gattttggaa taataccatt cctaaactat
      361 catataaaat aaagaaataa tataaaagtt ggaggaatag attacatgaa ctatgaaata
      421 attacacccg tagtgacagc atttgataga agaggaagaa tagattataa agcaaatgaa
      481 gagattatag atttcctaat tgatggtgga gtaaatggga tcttagtatt aggatcttct
      541 ggggaattca cagaattagc gtttcaagaa aagaaagatt tttttgattt ctattcagaa
      601 tatgtggatg gaaggttgga tctttacgct ggaactggaa gtatgaaatt aaaagaaaca
      661 gttgagctat caaattatgt atcaaagaga gattacaaag gatcttttat aataggtccg
      721 tattattatg gtcaggacca ggaaaaacta ttaacatatt ataacaaatt agccaaatcc
      781 atagatggta atatatatat atataatttc cctgatcgaa ccggacattc cttgagtcat
      841 gatactatta aacagttggc agatatgaat tcaaatataa aaggattaaa agattctgta
      901 actgaaccgg gacacaccaa cctatgtata ttggcaacta aggagaataa ttttgatgtt
      961 tactcaggat ttgacgatca ttatctatac aatatagcgg caggtggaaa aggctgcata
     1021 tccgcattga gtaatattgc tcctgaatta tggagtagcc tagttaaatc tagcaatgag
     1081 ggagattttg aaaatacaat aagactacaa tcacttgtac atgaacttat gccactatac
     1141 aacatggata gcaatttttc ttatttattt aaacgtttaa tgatttatag gggattaaat
     1201 ttcgaaccta aatcaatatt cccattcgac caattagatg aagaggtatt tgttaaagct
     1261 aagaggattt tggatacagt gctagctaaa tttcgagaga tagattataa ataagaataa
     1321 tggagatata cgtgaatact taagggctgg ctg
//
LOCUS       DATGSI010000087         6972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_573912, whole genome shotgun sequence.
ACCESSION   DATGSI010000087 DATGSI010000000
VERSION     DATGSI010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573912"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /gene="murF"
                     /locus_tag="VK031_01285"
     CDS             <1..1304
                     /gene="murF"
                     /locus_tag="VK031_01285"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HLS52580.1"
                     /translation="QRATRLYVVNLILCGIILFIPAISYILLDNIFLFVIFIPIALVV
                     LLVQPDTMYLSNIIMVPIENRINMGYYRAAQGKIKEREDLTVVGVTGSFGKTSTKFII
                     GTILEEKYNLLNTPESYNTPMGLSKVINNELDDKHEVFVAEMGARQIGEIKEVAELCQ
                     PRIGVLTSIGPVHLETFQNIDNIMKTKYELIEELPTDGVAIFNYDNEYIKKLADKTFK
                     EKILFGIDDVENLDIYADDIEVSELGSSFTLRDKEGNSKRCSTKLLGKHNIYNLLAGA
                     CVGRVLGLSFDEICEGISKVEPIPHRLNIINPGTGVIIIDDAFNSNPIGAKAALDVLS
                     QFKGGKKIIVTPGMVELGEMEEEANREFGNNIGKVCDYAILVGEKRTKPIYEGLMEVN
                     YNPENIFVVNNLEEASQHIGRLAKPKDVILFENDLPDNYSE"
     gene            1317..2480
                     /locus_tag="VK031_01290"
     CDS             1317..2480
                     /locus_tag="VK031_01290"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HLS52581.1"
                     /translation="MKKVGVIFGGRSVEHEVSVITGMQIMENMDKSKYQVVPIYIDKE
                     GKWFTGDCLLDFKNFRDNKLDDAEEIFVTATYKNNKLYADPEKVGLFGKKLLGEIDVV
                     IPALHGTHGEDGTIQGLFELIDIPYAGGGVLASSVGMDKILMKDVFRANNLPIVDYYW
                     FYRSNWEKNSETILEEIEDQLEYPIFVKPANLGSSIGINIARDKDALLEAIEVAVKYD
                     RKIIIEQAVVDPREINCAVLGYDEELITSLCEEPLGWEDVLTFEDKYVKSNAKGGKSA
                     GERRIIPAEISDELREKIEDLAKRSFKAIDGRGTARIDFLVDQEENVYVNEINTIPGS
                     IGFYLWEGKGYPFDKLIDELINIALIAHEDKKKNMYNFEANLFNRISYGGKVK"
     gene            2577..4697
                     /gene="rnr"
                     /locus_tag="VK031_01295"
     CDS             2577..4697
                     /gene="rnr"
                     /locus_tag="VK031_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HLS52582.1"
                     /translation="MSIRQKIVELMNSKDYKPMFKEELLAHFNIEIEFRREFYKILDS
                     LEREGLIIKTSKDKYAPINSQYLVAGVLQGHERGYGFVIPDEPLREDIFIPIDGMAGA
                     MHGDRVVANILKRSESGKREEGAIVRILERANKRVVGTFEDNKAFGFVIPDDQRIYND
                     IFVPKGAVNGARNRQKVVVEITKWPEPRRNPEGRIVEILGYMDEKGTDVLSIIRQFKL
                     PEKFPKKVVEYASSIGSTISEEDLEGRVDLRHLKTFTIDGADAKDLDDAVSIEKLDNG
                     NFLLGVHIADVAHYVKENSVLDKEAYKRGNSVYLIDRVVPMLPKELSNGICSLNPNED
                     RLTMSVIMEIDRSGRVVEHKILESVIRSHARLVYEDVSDFLEEDDEEAKEKLEDVLEE
                     LKLMEELMHILYKKRERRGSIDFDFPETFIDLDEKTGKPIDIKREERRVANRMIEEFM
                     LITNETIAEQFFWAEVPFLYRIHETPNEEKMASFARFIHNFGYQLKGQEIHPKELQLL
                     TKEIRGKKEEIVISTLMLRSLKKARYSSESDIHFGLASKYYSHFTAPIRRYPDLVIHR
                     IIKAFINGKLNLDKQDKLEETLPEVAAHTSMTERRAEEAEREVDDLKKTQYMSERIGE
                     VYEGIISSLTNFGMFVQLDNTVEGLVHFNQMLDDYYHFDEDNYYIIGERTKRIYRLGD
                     TVKVEVVAADIDRRAIDFQLVEED"
     gene            4752..5219
                     /gene="smpB"
                     /locus_tag="VK031_01300"
     CDS             4752..5219
                     /gene="smpB"
                     /locus_tag="VK031_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HLS52583.1"
                     /translation="MKKGGVKVVASNRRARHEFFIEDTIEAGIVLKGTEVKSIRQGRI
                     NIRESYASIENGEAFIHGMHISPYEQGNIHNVDPVRVRKLLLHKREIRRLAQATSLKG
                     YTLVPLSVYFKDGRVKIQLATAKGKKLYDKREDIARRDAERRMRQQSSEKYGI"
     gene            complement(5271..6206)
                     /locus_tag="VK031_01305"
     CDS             complement(5271..6206)
                     /locus_tag="VK031_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HLS52584.1"
                     /translation="MSDRTDLLIRVASYYYEDDMTQTDIAKVLDLSRPTVAKMLKEAR
                     EKGIVRITIEKRFSKSNELNRKQDHIARKYDLDTVLITESGSTDDETKARIGDLCARF
                     VEDRLDKIETLGISWGTTLKAYVDAAGYFNCPHLSIVPMIGGVGLSEIEWHSNHLAFT
                     LGQKYNAKSSFFYAPAIAENIEIKQALYNSQIIQEVLEAGRNVDLAIIGVGNPRQSKT
                     YRDMGYISELEEKEIINRDVIGDILTTFYNSEGEPVETSLSERMIGPSINDIKNIKDI
                     VVIASGESKIISIQALLKVDIIDHLIIDEEIANHL"
     gene            6357..6878
                     /locus_tag="VK031_01310"
     CDS             6357..6878
                     /locus_tag="VK031_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HLS52585.1"
                     /translation="MKLFKKEIQLQSQAGKATYIDITEQVNEAIKESEIEEGMVTVIS
                     PHTTCAVFFEEFSHDFNEEGDEFLQEDLNNVLEKIIPKHDSASRYKYPGEKHYEAVFS
                     WQNAEEYIPGGDKSALWNGDAHLKATIIGSSESFDVTGNRLGVGKTGYVYFVDFDDTR
                     PRTRKCKIIVIGK"
BASE COUNT         2513 a         1013 c         1522 g         1924 t
ORIGIN      
        1 cccaaagagc caccaggctg tatgtggtga atttaatcct ttgtgggata atcttattta
       61 ttcctgcaat atcctatatt ttgttagaca atatatttct atttgttata tttattccta
      121 tagccctggt agttttacta gtacaaccag acactatgta cctatctaac ataattatgg
      181 tacccataga aaacagaata aatatgggct actatagggc tgcccaaggt aaaataaagg
      241 aaagagaaga tctgacagtg gtaggggtta caggtagctt tggaaagacc agtacaaaat
      301 ttattatagg gactattctg gaggaaaaat ataatctttt aaatacgcct gaatcttata
      361 acactcccat gggtttatct aaggtaataa acaatgaatt agacgataaa catgaggtgt
      421 ttgttgctga aatgggagca agacaaattg gtgagattaa ggaagtggca gaactgtgcc
      481 agcctaggat aggagtgctt acatccatag gaccggtgca tctggaaacc tttcaaaaca
      541 tagataatat aatgaaaaca aaatatgaat taatagaaga attacctaca gatggggtag
      601 ccatctttaa ttacgacaat gagtatatta aaaagttggc agataaaacc tttaaagaaa
      661 aaatactttt tggtatagat gatgtggaaa acctggacat atatgcagat gatatagaag
      721 tatctgaact aggctctagc ttcaccttaa gagataagga aggaaatagc aaaaggtgta
      781 gcacaaaact tttaggcaaa cataatatat ataatttgtt ggcaggagcc tgtgtaggca
      841 gggtattagg cctaagcttt gatgaaatat gtgaagggat aagcaaggta gaaccaatac
      901 ctcatagatt gaatattata aatcctggaa caggggttat aatcatagat gatgccttta
      961 attctaaccc tataggagca aaggcagcct tagatgtgct aagccagttt aagggtggta
     1021 aaaagattat agtaactcca ggtatggttg agttgggaga aatggaggaa gaagccaata
     1081 gagagtttgg aaataatatt ggaaaggttt gtgactacgc aattttagtg ggagaaaaga
     1141 gaacaaaacc aatatatgaa ggcctaatgg aggtaaacta taatccagaa aatatatttg
     1201 ttgtaaataa tttagaagaa gcatctcaac atataggtag acttgctaaa cctaaagacg
     1261 taatcttatt cgagaatgat ttaccagata attatagtga atagggaggc ttttaaatga
     1321 aaaaggtagg agtaattttc ggtggtagaa gtgtagaaca cgaggtttct gtaattacag
     1381 gaatgcaaat tatggaaaat atggataagt ccaagtacca agtagtgcct atctatatag
     1441 ataaggaggg gaaatggttt actggagatt gtttattaga ttttaaaaac ttcagggaca
     1501 ataagctgga tgatgcagaa gaaatatttg taacagccac ctacaaaaat aataagcttt
     1561 atgcagatcc agagaaggtt ggcttgtttg gaaaaaaatt gctaggtgaa atagatgtgg
     1621 taatcccagc cctacatggt acccatggag aagatgggac catacaaggc ctatttgaac
     1681 tgatagatat tccttatgca ggtggaggag tattagcatc aagtgtaggc atggataaga
     1741 tacttatgaa ggatgtgttt agggccaaca atttacctat agttgactat tattggtttt
     1801 ataggtccaa ttgggagaaa aatagtgaga ccatactaga agaaattgaa gaccagctag
     1861 aatatccgat ttttgtaaaa ccagctaact taggctccag tataggaatc aacatagcaa
     1921 gggataagga tgccctactt gaagcaattg aagttgcggt taaatatgat aggaagataa
     1981 tcattgagca ggcagttgta gacccaaggg aaattaattg tgccgtgctt ggctatgatg
     2041 aagaacttat tacatcactt tgtgaagagc ctttaggctg ggaggatgta ttaaccttcg
     2101 aagataaata cgtaaaaagc aacgcaaagg gcggtaaatc tgctggtgaa agaaggataa
     2161 ttccagctga aatctcagat gagttacggg aaaagataga agatctggct aaaagatcct
     2221 tcaaggccat agatgggaga gggacagcca ggatcgactt tttagttgac caagaagaaa
     2281 atgtatatgt aaatgaaata aataccatac ctggttccat aggtttttac ttatgggaag
     2341 gaaagggata tccatttgat aagttaatag acgaattaat aaacattgca ttaatcgctc
     2401 atgaagataa aaagaaaaac atgtataact ttgaagctaa cctatttaat cgaatttcat
     2461 atggaggcaa ggtaaaataa tgtaaggtat ttaatatctt acattatttt actttaaaag
     2521 ggtaaatact ataggatagg agtgtaatat tattacctct aaaaggagga tagtaaatga
     2581 gcataagaca aaaaattgtg gagttaatga attccaaaga ttataaacca atgtttaaag
     2641 aagagctact ggcacatttt aatattgaaa tagaatttag aagagaattt tataaaatat
     2701 tagatagcct ggaaagagaa ggactaataa taaaaacttc aaaggataag tatgccccaa
     2761 taaatagcca gtatttggtg gctggtgtat tgcagggaca tgagagaggt tatggctttg
     2821 taattccaga tgagccttta agggaagata tcttcattcc tatagatggt atggctggcg
     2881 caatgcatgg agatagggtt gtggccaata tacttaaaag gtctgaatca ggaaagaggg
     2941 aagaaggggc aatagtaagg attttagaaa gggctaataa aagggtcgta ggaacctttg
     3001 aagataataa ggcctttggt tttgtaatcc cagatgatca aagaatttat aatgacatat
     3061 tcgtgcctaa gggggccgta aatggggcta gaaatagaca aaaagtagtg gtagaaataa
     3121 ccaaatggcc tgaacctaga agaaatccag aaggtagaat agtagagatt ctaggttata
     3181 tggatgagaa ggggacagat gttttatcca taattaggca atttaagcta ccggaaaagt
     3241 ttcctaaaaa ggtagtggaa tatgccagca gcataggaag tactatttca gaagaggatt
     3301 tggaaggtag agttgatcta cgtcatttaa aaaccttcac cattgatggt gcagatgcaa
     3361 aggacttaga tgatgcggtt tccatagaaa aacttgataa tggaaacttc ctattgggag
     3421 tccatatagc cgatgtggcc cattacgtta aggaaaattc agtcttagat aaggaggcct
     3481 acaaaagggg caactctgtt tatctaatag accgggtagt acctatgttg cccaaagaac
     3541 tatctaatgg gatttgttca ttaaatccta atgaagatag gctcaccatg tcagtaatca
     3601 tggaaataga taggtcagga agggttgtag aacacaagat cttagaaagc gtaataagga
     3661 gccatgccag gctagtttat gaagatgtat ccgatttttt agaagaagat gatgaagaag
     3721 ctaaggaaaa attagaagat gttttagaag aacttaaact aatggaagag ttaatgcata
     3781 ttttatataa aaagcgggag agaaggggaa gtattgattt tgattttcct gaaaccttta
     3841 ttgatctaga tgaaaaaaca gggaagccta tagatataaa gagggaagaa agaagagtag
     3901 ccaataggat gatagaggaa tttatgttaa taactaatga aactatagct gaacagtttt
     3961 tctgggcaga ggttccattt ttatatagga tacatgaaac tcctaatgaa gaaaaaatgg
     4021 ctagttttgc tagatttatc cataactttg gttatcaact aaaggggcaa gagatccatc
     4081 ctaaagaact gcaactgtta actaaggaaa taagggggaa aaaggaagaa atagtaataa
     4141 gtacccttat gttaaggtcc ttaaaaaagg ctagatacag tagtgaatct gacatacatt
     4201 ttgggctagc atctaaatac tattcccact ttactgcccc aattagaagg tatccagatc
     4261 tggtgattca taggattata aaagccttta taaatgggaa gcttaattta gacaaacagg
     4321 acaaattgga ggaaacttta ccagaggtag cagcccatac ctccatgact gagaggcggg
     4381 cagaggaagc tgaaagagaa gtagatgatt tgaagaagac ccagtatatg agcgaaagaa
     4441 ttggggaagt ctatgaagga ataatatctt cattgacaaa ctttggtatg tttgttcaat
     4501 tagacaatac agtggagggg ttggttcatt ttaaccaaat gttagatgat tattaccatt
     4561 ttgatgaaga taattattat ataataggag agagaacaaa aagaatatac aggctgggag
     4621 atacagtaaa ggtagaggta gtggcggcag atatagatag aagggccatt gattttcaac
     4681 tggtagagga agattgacag gtgaaaatat atttgctaca ctaaactgga caataggtta
     4741 agggtgataa aatgaaaaag ggtggagtaa aggttgtagc aagtaataga agggctagac
     4801 acgaattttt catagaagat actattgaag caggaatagt cctaaaagga acagaggtta
     4861 aatcaattag gcaaggtaga atcaatatta gagaaagtta tgccagcatt gaaaacggtg
     4921 aagcgtttat tcacggtatg catataagcc cttatgagca aggaaatatc cacaatgtgg
     4981 atccagtaag ggtcaggaag ctacttttgc acaagcggga gataagaaga ttggcccagg
     5041 ccacatctct taaggggtat accttagttc ctttgtctgt gtactttaaa gatggaaggg
     5101 taaagatcca actggcaacg gctaaaggaa agaaacttta tgataagagg gaggatatag
     5161 ccagaaggga tgcagaaaga agaatgagac aacaatctag tgaaaaatat ggaatataaa
     5221 caagggggca gaccatttgg tctgtctttt atattcccca ttttatattc ttacaggtga
     5281 ttggctattt cctcatcgat aattaaatga tctattatat ccacctttaa tagggcctgg
     5341 atgctgatta tcttagattc tccactggct ataaccacta tatcctttat atttttaata
     5401 tcattaatgg acggtccaat cattcgctca gatagagaag tctcaactgg ttcaccttca
     5461 ctgttataaa aggtggtcaa aatgtcaccg attacgtccc tatttattat ttctttttct
     5521 tccagctcag aaatataacc catatcccta taggttttag actgacgagg attaccaaca
     5581 cctattatag ccagatccac attccgaccg gcttcaagca cttcctgaat aatctgggaa
     5641 ttataaaggg cctgtttaat ttcaatattt tcagctatgg caggtgcata gaagaaactg
     5701 gacttagcat tgtatttttg tcctaaggta aaggccagat ggttagaatg ccactctatt
     5761 tcagataggc ctaccccacc aatcataggt actatagata gatgagggca gttgaaatac
     5821 cctgctgcgt ccacataggc ctttaaggta gttccccagc taatacctag agtttctatt
     5881 ttatccaacc tatcttctac aaacctggca cataaatccc caatcctagc cttagtttca
     5941 tcgtcagtac taccagattc agtaattaaa acagtatcta aatcatattt cctggctata
     6001 tggtcctgct ttctatttaa ttcattgctc ttggaaaaac gtttctctat tgtaatacga
     6061 actatgccct tctccctagc ctcctttaac attttagcca ccgtaggtct agacagatcc
     6121 aatacctttg caatatcagt ttgagtcata tcatcttcat aatagtaact agctacccta
     6181 atcaacaagt cagttctatc actcaattgt aagccacctc caaaatatac tgggaaacta
     6241 tttaacaaat gcaaaacatg caaaaaaact ttacaaatgt ttgacaaata ccctattaaa
     6301 ttatattatt taggtagaag caaatataat gttattataa aaataggagg atgtaaatga
     6361 aattatttaa aaaggaaatc caactacaat cccaagctgg caaggccaca tatatagaca
     6421 ttactgagca agtaaatgag gcaattaagg aaagcgagat agaagaggga atggtgacgg
     6481 taatttcacc gcataccaca tgcgcagtat tctttgaaga attctcccat gattttaatg
     6541 aagagggaga tgagttccta caggaagatt taaacaatgt tttagaaaaa atcatcccaa
     6601 aacatgacag tgccagcagg tacaaatacc ctggagaaaa gcattacgag gctgtattta
     6661 gttggcaaaa tgctgaagag tatattcctg gtggagataa aagtgccttg tggaacggag
     6721 atgctcatct aaaggcaact attataggtt ccagtgaatc ctttgatgta actggaaata
     6781 gactaggtgt aggtaaaact ggttatgttt attttgtaga ctttgatgat acaaggccca
     6841 gaaccaggaa gtgtaagata attgtaatag gtaaatagtt ctgaaatcaa ggaaaagagg
     6901 gatagaatgt tagtaaattc aaaagaaatt ttattggagg aaagaaaaag aggctttgcc
     6961 ataccttcag cc
//
LOCUS       DATGSI010000088         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_620672, whole genome shotgun sequence.
ACCESSION   DATGSI010000088 DATGSI010000000
VERSION     DATGSI010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620672"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VK031_01315"
     CDS             <1..222
                     /locus_tag="VK031_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS52586.1"
                     /translation="QKQRVAIARALAMEPQFICFDEPTSALDYKLRDSVGDLIETVAK
                     QGMGVIVITHDRDFAKTYAHRSFELKPLQ"
     gene            311..1552
                     /locus_tag="VK031_01320"
     CDS             311..1552
                     /locus_tag="VK031_01320"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HLS52587.1"
                     /translation="MTYIKNKGILLSKASRALSKASTLEKNQALRLVIDSINDHRDNI
                     LRANEQDIKAGKENNMGDALIDRLFLDQERLDGIIEGIETVIKLEDPIWQSDKVWTLE
                     NGLTISKMTVPLGVIGIIYESRPNVTVDAFSLALKSGNCILLRGSSSAINSNTALVKA
                     IKEGLSKSTISADVLQFIEDTDRSLVLDMLRANEYLDLIIPRGGKFLIDFVTKNATVP
                     TLQTGEGNCHIFVDQSANLDQALEILDNAKTQRMGVCNACETLLVHENIKDSFLQMVY
                     NKLKGKVQLRGCQVSRTIIDIEPATEADYYEEYLDAILAIKIVKDVDEAIFHINKYGT
                     QHSDAIITENLTNANRFQREVDSSSIYVNASTRFTDGGEFGFGAEMGISTQKIHARGP
                     VALKELTTYKYLVMGQGQIRK"
     gene            1561..2355
                     /gene="proB"
                     /locus_tag="VK031_01325"
     CDS             1561..2355
                     /gene="proB"
                     /locus_tag="VK031_01325"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HLS52588.1"
                     /translation="MKEIVKESEKIVIKIGTSTITSDDGTIDRTFMDNLAQQVKMLKD
                     QGKRIIIVSSGARIAGVSTIGKWSRKEDINYKQALCAIGQVELMDSYRRIFSQYGIHV
                     AQILLTKEDFESENRTLNIRNTLFTLLDEGVIPIINENDTVCVEEIKIGDNDVLAART
                     TALWGGDLLILLSDIEGVYDKNPQSNDDAILIERVLDPYELENNISIGEKGSFGTGGI
                     ATKIKAAQIVNKYGASLIVAKGKEEDIIIKLGEGKAKGTLFQTNIS"
     gene            complement(2381..2482)
                     /locus_tag="VK031_01330"
     CDS             complement(2381..2482)
                     /locus_tag="VK031_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HLS52589.1"
                     /translation="MDQEVVVDGHLITSRTPKDLPIFLKTIIEKLKE"
BASE COUNT          839 a          392 c          517 g          737 t
ORIGIN      
        1 caaaagcaaa gggtggccat tgccagagcc ttggccatgg aaccacagtt tatatgtttc
       61 gatgaaccta cttctgccct tgattataag cttagggata gtgtgggaga cttaattgaa
      121 accgtggcca aacaaggtat gggtgttatt gtaataaccc acgatagaga ctttgcaaaa
      181 acttatgccc ataggagctt cgaattaaag cctttacaat aaatttttat aaatatacgc
      241 ctttcttagt ctaatgactt aattcattaa tatgatataa tagtataaaa ttctattagg
      301 ggggattctt ttgacctata ttaaaaataa gggaatcttg ttaagtaagg cctccagagc
      361 cctatctaag gcctccacct tagagaaaaa tcaggccttg agactggtta tagattccat
      421 taacgatcat agagacaata ttttaagggc caatgagcag gatattaaag ctggaaaaga
      481 aaataatatg ggtgatgcct taattgatcg cctattcttg gaccaagaac ggctggacgg
      541 aataattgaa gggattgaaa ctgttattaa attagaggat cctatttggc aatcagataa
      601 ggtatggacc ttagaaaatg gtcttactat ttccaaaatg acagtaccct taggtgttat
      661 aggtattata tatgagtcta ggcctaatgt tactgtagat gcctttagtc tagccctaaa
      721 atcaggtaat tgtattcttt taagaggtag ctcctcagct attaactcta acactgccct
      781 ggttaaggct ataaaagaag ggcttagtaa atctacaatt tccgctgatg tacttcaatt
      841 tattgaagat actgatagga gcttggtttt agatatgcta agggccaatg aatatctaga
      901 tttaattatt cctagaggtg gcaaattctt aatagatttt gttaccaaaa atgcaactgt
      961 tcctacctta caaactggag aaggaaactg tcatattttt gtagatcaat ctgccaatct
     1021 agatcaggcc ctggagattc tagacaatgc taaaactcaa aggatggggg tttgcaatgc
     1081 ttgtgaaacc ttattagttc atgaaaatat taaagattcc ttcctccaaa tggtatataa
     1141 taagcttaaa ggtaaggttc agttaagggg atgtcaagtt agcagaacta taatagatat
     1201 tgagccggcc acagaagctg actattatga ggaatattta gatgctatct tggcaataaa
     1261 gatagttaag gatgtggatg aggcaatttt tcatataaat aaatatggta cccaacactc
     1321 agatgctatt attaccgaaa atcttactaa cgccaatagg tttcaacggg aagtggattc
     1381 atcctccata tacgtaaatg cttctactag atttacagat gggggagaat ttggcttcgg
     1441 agctgagatg ggcattagta ctcaaaaaat acatgctaga ggacctgttg ctttgaaaga
     1501 gcttacaacc tacaagtatt tagtaatggg tcaaggtcaa attagaaagt aggaatagat
     1561 atgaaagaaa tagtaaagga aagcgaaaaa attgttataa aaattggaac cagcaccatt
     1621 accagtgatg atggtactat tgataggact tttatggata atttagccca acaggtaaag
     1681 atgcttaaag atcagggtaa aagaatcatt atagtctctt ctggggctag gattgcaggt
     1741 gttagtacca taggtaaatg gtctagaaag gaagatatca actataagca ggccctatgt
     1801 gccataggtc aggtggagct aatggactct tatagaagga tatttagcca gtacggaatt
     1861 catgtggccc aaatccttct tactaaggag gactttgaat cggaaaatag gaccttaaat
     1921 attagaaaca ccttgtttac cctactggat gagggtgtta tacctataat aaatgaaaat
     1981 gatactgttt gtgtagagga gattaaaata ggtgacaatg atgttttagc tgccaggact
     2041 actgccttat ggggcggtga cttacttata ctcttaagtg atattgaggg agtctatgat
     2101 aaaaatccac agtccaatga tgatgcaatc ctaattgaac gagtccttga tccttacgaa
     2161 ttggaaaata atatatccat aggggaaaag ggctcctttg gaactggtgg tattgctacc
     2221 aaaattaagg cagctcaaat cgtcaataag tacggagcct ccctaatagt tgccaaaggt
     2281 aaggaagaag acataataat aaaattaggt gaaggcaagg ctaaaggcac cttattccaa
     2341 acaaatatat cataaaaaac tacagcatgg ctgtagtttt ttattcttta agtttttcta
     2401 ttatggtttt taaaaagatt ggtaaatcct ttggtgtcct agaagtaatt aaatgtccat
     2461 ctactaccac ttcttggtcc acata
//
LOCUS       DATGSI010000089         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_620740, whole genome shotgun sequence.
ACCESSION   DATGSI010000089 DATGSI010000000
VERSION     DATGSI010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620740"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2030)
                     /locus_tag="VK031_01335"
     CDS             complement(<1..>2030)
                     /locus_tag="VK031_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLS52590.1"
                     /translation="VEAVGQFSLRGGIIDFFPAHSQNPYRIELFDEEVDSIRSFDILT
                     QRSLEIVDKVTVTPVEEILIVDKFKKQVILNLEKDLKKHEGNERIKEKFNKYMDQLEE
                     GGSTNNQMVQAYIPDEFLTSIIDYISREALIFLDEPGRIREKAEDIQEDRRLKLTDLL
                     EAGEVMISHFHMWYKYEELLGKLEKKLLIINSSLLRDPGDFSLKGIYNFTVKSTINYH
                     NKLGLLTEDINHLLYRGYKVIILAGTKDRGLRLQKDLQDFNINSSFFEKRVGRIKSGQ
                     LFITIGSLKKGFEYVDNKLIVISDKEIFGAQKKKRGRSKKRKDQKVLNIADLNVGDYI
                     VHENHGIGRYEGIEQLDIQGIKKDYLTIRYRDQDKLYIPIDQMNLIQKYVGSDAAKPR
                     INRLNSGEWQRTKGRARKAVEDMAEDLLKLYAKRANLKGHAFSKDSPWQKQFEDLFPF
                     EETEGQINSTLEIKKDMEKPKPMDRLLCGDVGYGKTEVALRAAFKALIDGKQVAILVP
                     TTILAQQHYNTIIDRFGDFPVEVALLSRFRTAKEQKQTIEGLKNGTIDMVVGTHRLLS
                     KDISFKDLGLLIIDEEQRFGVKHKEKLKKFRESIDVLTLTATPIPRTLHMSLVGIRDM
                     SVIEEPPEERYPIQTYVLEYNPQMIRSAILKEIDRGGQVYFVYNRVETIEKM"
BASE COUNT          536 a          463 c          281 g          750 t
ORIGIN      
        1 catcttctct atggtctcta ccctattgta gacaaagtat acctgcccgc ctctatctat
       61 ttccttgaga atagcacttc ttatcatttg tggattatat tctaggacat aagtttgtat
      121 tggatacctt tcctctggtg gctcctcaat aacagacata tcccttatac ctaccaggga
      181 catatgtaaa gtccttggta taggagtagc agttaaagtt aatacatcta tgctttctct
      241 aaatttcttt aatttttctt tatgttttac tccaaagcgt tgttcttcgt ctattattaa
      301 taggcctaga tctttaaagc ttatatcttt agacaaaagc ctatgggtgc ctactaccat
      361 gtcaatggta ccatttttta gaccctctat agtctgtttt tgttccttgg ctgttctaaa
      421 tctacttagt agggccacct caactggaaa atctccaaac ctatctataa ttgtattata
      481 gtgctgttga gccagtatag ttgtaggtac caaaatagct acctgctttc catctattaa
      541 ggccttaaag gctgccctta aggccacctc agtttttcca taccctacgt ccccacacag
      601 tagcctatcc ataggctttg gcttttccat atccttttta atttctaatg tagaatttat
      661 ctgtccttct gtttcttcaa agggaaacaa atcttcaaat tgcttctgcc aagggctatc
      721 tttactaaaa gcatggccct tcaggttggc tcgcttggca tatagtttca gcaagtcttc
      781 cgccatatcc tccacagcct tcctggccct acccttggtc ctttgccatt ctccactatt
      841 taatcgattt attctaggct tggctgcgtc agatcctaca tatttttgga tcaaattcat
      901 ctgatcaata gggatgtata acttgtcttg atccctatat ctaatagtta gataatcttt
      961 tttaattcct tgaatatcta attgctctat accctcataa cgcccaattc cgtgattttc
     1021 atgaactata tagtcgccta catttaaatc tgctatgttt agaacttttt ggtccttccg
     1081 ctttttactt cgccctcttt tcttcttttg ggccccaaag atttccttat cactgataac
     1141 gattaattta ttatcaacat attcaaagcc tttcttcagg ctacctatgg ttataaataa
     1201 ttgtcccgat ttaatccttc caaccctttt ttcaaagaag ctactattaa tattaaaatc
     1261 ctgtaaatcc ttctgtagcc tcagtcccct atctttagta cctgccagta taattacctt
     1321 atagcccctg tagagaaggt ggttaatatc ctcagtcaaa agtcctaact tattatggta
     1381 atttatagtt gatttaacag taaaattata aattcctttt aaggagaaat caccaggatc
     1441 tcttaataag ctgctattga ttataagcag ttttttttcc agtttcccta ataattcttc
     1501 atacttatac cacatatgaa agtgggatat catcacttcc ccggcctcta acagatcagt
     1561 aagttttaat ctccgatctt cctgaatatc ttctgccttt tccctaattc gcccaggctc
     1621 atctagaaaa attaaggcct ctctactaat ataatcaatt atagaagtta aaaattcatc
     1681 tggaatatag gcctgaacca tttggttatt ggtgctgcct ccttcttcca gctgatccat
     1741 gtatttatta aatttttctt ttattctttc attgccttca tgctttttta agtccttttc
     1801 cagattaagt ataacctgtt ttttaaactt atccacaata agtatctctt ctactggagt
     1861 tacagtgacc ttatccacaa tttctaaaga tctctgtgta agaatatcaa agcttcttat
     1921 ggaatcaact tcttcatcaa ataactctat cctataaggg ttctgactat gggctgggaa
     1981 aaaatctatt atcccgcccc ttaggctaaa ctggcctact gcctccacca
//
LOCUS       DATGSI010000090         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_7721, whole genome shotgun sequence.
ACCESSION   DATGSI010000090 DATGSI010000000
VERSION     DATGSI010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7721"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VK031_01340"
     CDS             <1..276
                     /locus_tag="VK031_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLS52591.1"
                     /translation="FRNFPVEVGSKTGTAQREGINPITKEKYDEFSWFVGFAPYDDPE
                     IAVATVIFQGGSGSHGGPIVRDIIAEYLGLNKTGREENLPYENTISQ"
     gene            340..987
                     /gene="minC"
                     /locus_tag="VK031_01345"
     CDS             340..987
                     /gene="minC"
                     /locus_tag="VK031_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HLS52592.1"
                     /translation="MRNQIISFKGVAEGVKIVVKGSDLDMVKKALDKKIRRSIKFYQG
                     IKILGIEGEELPPEDTFELALILRYKYDLNIDLEELPLDFEEIFKKKRAEKTKEVERT
                     EEGPTRFFYGTLRSGQEVDYDGNIVVIGDVNPGALLKAKGNIIVLGSLRGVAHAGLGG
                     NKHSIVAAYNLLPTQLRIADTIVRAPDDDEAEIKLPEVARIHKGEVIIEPYLPNK"
     gene            1005..1817
                     /gene="minD"
                     /locus_tag="VK031_01350"
     CDS             1005..1817
                     /gene="minD"
                     /locus_tag="VK031_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HLS52593.1"
                     /translation="MEKEKQGTAIVITSGKGGVGKTTTCANIGTGLAMMDKSVVVVDA
                     DIGLRNLDVILGLENRIVYDIVDVVEKNCRLKQALIRDKRFENLYLLPAAQTKDKSAI
                     KPEQMLELVGDLKKEFDYIIIDSPAGIEQGFQYSIAGADLAVIVTTPEISAVRDADRV
                     IGILESRGLYDPKLIINRIRPDMVRRGDMMNMDDIVDILAIDLLGIVPDDEEIVISAN
                     KGEPVVIEGVSLAGRAYNNISQRIAGDEIELLNLEDNNESKFSKIMKLLFGK"
     gene            1830..2108
                     /gene="minE"
                     /locus_tag="VK031_01355"
     CDS             1830..2108
                     /gene="minE"
                     /locus_tag="VK031_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HLS52594.1"
                     /translation="MDFLKIFGRKEEKSKNLAKERLKLVLVHDRSDLSPKFLDMMKSD
                     IVRVISEYADIDEKALDIKLTRMKRDEGEAPISALVANIPIIKVRHKE"
BASE COUNT          784 a          327 c          504 g          520 t
ORIGIN      
        1 tttagaaact tccctgtaga agtaggttca aaaacaggta cagcccaaag ggaagggata
       61 aatccaatta caaaagaaaa atatgatgaa ttttcctggt ttgtaggatt tgcaccctat
      121 gacgatcctg aaattgcagt agccacagta atcttccagg gaggtagcgg aagtcatggc
      181 ggaccaattg taagagatat aattgcagag tatttaggat taaataaaac aggaagagaa
      241 gaaaatctac catatgaaaa tactatatcc caataaaaaa gaaggatttt ttaaatcctt
      301 atagaatata tatggtaggg tctaatggag gttaggctag tgaggaacca aatcataagt
      361 tttaaaggcg tagcagaagg tgtaaaaata gttgtaaaag gttcagacct agatatggta
      421 aagaaggccc tggataagaa aatacgtagg tctattaagt tttaccaagg aattaaaatc
      481 ttaggcatag agggggaaga acttccccct gaagatacat ttgaattggc cctgatttta
      541 agatataaat acgacctaaa tattgatctg gaagaattgc ccctagactt tgaagagatt
      601 ttcaaaaaga aaagggccga aaaaaccaaa gaagtggaaa gaactgaaga aggacctact
      661 aggttttttt acggaaccct aagatctgga caagaagtag actatgatgg caatatagta
      721 gttataggag atgtaaatcc tggggccctt ttaaaggcca agggtaatat aatagtctta
      781 ggtagcctaa ggggggtggc ccatgcaggc ctgggaggca ataaacactc catagtggca
      841 gcctacaacc tgcttccaac ccagcttaga atagcagata ccatagtaag agccccagat
      901 gatgatgaag cagagattaa actgccagaa gtggcccgta tccacaaggg tgaagtaata
      961 atcgaaccat atttaccaaa taaatagatg atggagggaa tggaatggaa aaggaaaaac
     1021 aaggaacagc catagttatc acctcaggta agggaggagt tggcaaaacc accacctgtg
     1081 ccaacatagg tactggcctt gccatgatgg acaagtctgt tgtagtggta gatgcagaca
     1141 taggcctgag aaacctagac gttattttag gattagaaaa tagaatcgta tatgatatag
     1201 tggatgtggt ggaaaaaaat tgtcgcctaa agcaggccct aattagggac aaaaggtttg
     1261 aaaacctata cctgctacca gcagcccaaa ccaaggacaa gtctgcaata aagcctgagc
     1321 agatgttgga gctggtaggt gatttgaaaa aagagtttga ctatataatc atagactcac
     1381 ctgctggtat agagcagggc ttccaatact ccatagctgg agcagatttg gcagtaatag
     1441 taactactcc agaaatttct gcagttagag acgcagacag ggtaataggt atactggaat
     1501 ctagaggcct atatgatcca aaattaatca tcaacaggat caggccagat atggttaggc
     1561 gaggagatat gatgaatatg gatgatatag tagacattct agccatagat ttattaggta
     1621 tagtgcctga tgatgaagag atagtaatct cagccaacaa gggtgagcca gtagttatag
     1681 aaggagtctc tctggcaggc cgggcctata ataatatttc acaaagaatt gctggagacg
     1741 aaatcgaatt attaaactta gaggataaca atgaaagtaa atttagcaaa ataatgaagc
     1801 tgctatttgg taaataagga ggaatagtct tggattttct aaaaatattt ggtagaaaag
     1861 aagagaaaag caagaatctt gctaaggaaa gactgaaact cgtattagtt catgatagat
     1921 ccgatctgtc acctaagttt ttagacatga tgaaaagcga tatagttagg gtcatatccg
     1981 agtatgcaga tatagatgaa aaagccttag atataaaatt aactagaatg aagagagatg
     2041 aaggtgaagc ccccatatct gccttggtgg ccaatattcc cataatcaag gtaaggcata
     2101 aggaatagaa ggggttttgt aaggattatc aaata
//
LOCUS       DATGSI010000091         5395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_101643, whole genome shotgun sequence.
ACCESSION   DATGSI010000091 DATGSI010000000
VERSION     DATGSI010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101643"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..815
                     /locus_tag="VK031_01360"
     CDS             39..815
                     /locus_tag="VK031_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HLS52595.1"
                     /translation="MYRLLNINRTGLRAIETKVDSLADNLSNIETYGYKAKDVTFQEL
                     LTNQVHNNDVLLSGNVNHSRINAGSKSSLTGINFQQGNITPSTGDFHLAIEGDGFFGV
                     YDRDGNLMLTRNGGFHINADKTITDDNGNPLSMELYVPFDQWEVSQVKISNNGLITTN
                     INGEPIELGRVILYRPEVLDSLTPLGEGRYMPAANLPLYNSLESDNFGQIIQHALEAS
                     NVELAKSMTELIIAQRAYSFNAKSLENTDEIMSLINNIKR"
     gene            881..1636
                     /locus_tag="VK031_01365"
     CDS             881..1636
                     /locus_tag="VK031_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HLS52596.1"
                     /translation="MNAALAEIATLEGTKFMKPIIGITSRDDYTGRIKINLVNHTYID
                     AVIEAGGIPLVLANLQDLDDIDGYIKTLDGIIFSGGEDISPLVYGENPIKEVKNISYR
                     RDKFELELFNRAYGKGLPILGICRGIQLVNVALGGSLYQDIHQQIPQALGHVSTYRIE
                     GGYHTIDIKRDSILFDIFKEKKIQVNSQHHQSVKDLGDNLRVSARAQDGVIEAIESSN
                     DNFVLGVQFHPEAMVSEHKEFVCIFEYFINQCK"
     gene            complement(1663..3432)
                     /locus_tag="VK031_01370"
     CDS             complement(1663..3432)
                     /locus_tag="VK031_01370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HLS52597.1"
                     /translation="MRWSLDSLYPSFDSEEYKRDLRKLDQLIKEIQVWADENLSSLNG
                     AGEKIESYINYSQTLSSLFTRLMSFANLKITVDARDEEAIQYLDRLRIKSTEITKPEV
                     QFQNFIKEIDNLDGLISSSDLLKEHSFYLRQIKDRSRYLLSEKEEILISKLSNTGSTA
                     WSNLQDRLTSTLIVDIYIDGEDKQLPLSVVRNMAYDKDPNIRKLAYEAELKAYKKVEE
                     SSAAALNSIKGEVLTTSELRGFESPLEETLFKSRMDKESLDAMLTAMEEFLPVFHKYY
                     RKKAEILGHKNGLPFYDLFAPVGQVNMTFGYDEAMEYIVDNFRSFSDRLANFVDKAYR
                     NKWLDVEPREGKVGGAFCANLHPIGESRILANFDGSFSNMITLAHELGHAYHGFNLKD
                     ESILNSRYPMPIAETASIFCETIVTNAALREADQQEALSILEASISDAGQVIVDIYSR
                     YLFETELFNRRKNYSLSVNELKEIMIDAQKQAYGNGLDHDFLHPYMWLNKSHYYSAGN
                     NFYNFPYAFGLLFSKGLYAEYLNRGQEFIKEYDDLLNATGKNNIRDVGLRMGIDVHNP
                     DFFRNSLRLIEKDIERFIELADK"
     gene            3621..5216
                     /locus_tag="VK031_01375"
     CDS             3621..5216
                     /locus_tag="VK031_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLS52598.1"
                     /translation="MKKLNFNQIDLKDEILRGISDMGFEEMSPIQEQAIPKILKGQDI
                     IGQAQTGTGKTAAFGIPLLEMCDPNNRSLQALILCPTRELSIQVSEEIRRIGKYTRGI
                     STLPVYGGQPIDRQIRALKRGVQIVVGTPGRVIDHIRRRTLRLDQLKMMVLDEADEMF
                     DMGFRDDIGLVMDKLPEERQTIFFSATMPKEIINFASRYQKDPEIVRVVHKELTMPKI
                     EQYYFELKEHMKTEILSRLIDIYNPRLSIVFCNTKRKVDELTEKLVARGYLADGLHGD
                     LRQPQRDTVMNKFRRGSIDILVATDVAARGLDVDDIDLVINYDIPQDEEYYVHRIGRT
                     ARAGREGIAFSFVVARDKNKLRNIQKYAKTTIRRKDLPTLKDMEEVYRSSLLDNIKEE
                     ISKGKLGKYNKIVDLLIEEGYTSFDIAAALLKFHMEANKYNKHEELDMVDYGQPLKST
                     AMARLYINIGSRKGASPRHILAAIVQETGIKRTKVGTIDVYDKFSFIEVPADQAKFIM
                     NRLNKKRIRGSRVRVEMANPKRK"
BASE COUNT         1851 a          952 c         1033 g         1559 t
ORIGIN      
        1 gcaaggctgt aaatgaaata ggaagggtgt aggatataat gtatagactt ttaaatataa
       61 ataggacagg ccttagggca atagaaacta aggtggacag cctggcagat aatctatcta
      121 atatagaaac ctatggatat aaggccaagg atgttacctt ccaagagcta ttaaccaatc
      181 aagttcataa taatgatgtt ttgttatctg gcaatgtaaa tcacagcagg attaatgctg
      241 gtagtaaatc aagtttaact ggtatcaact ttcagcaagg taatattaca ccttccactg
      301 gagattttca cctggccata gaaggagatg gtttctttgg cgtctatgac agggatggaa
      361 acctaatgtt aacaagaaat ggtggctttc atatcaatgc agacaagact attacagacg
      421 acaatggtaa tccactaagt atggagcttt atgtaccctt tgaccaatgg gaggtaagcc
      481 aggttaaaat atcaaataat gggcttatta ccaccaatat aaatggagag cctatagaat
      541 taggcagggt aatcttatat aggccagaag ttttagattc cttaaccccc ctaggagaag
      601 gtagatatat gccagcagcc aacctacccc tatacaattc cttagaatct gataactttg
      661 gccagataat tcaacatgcc ttggaggctt ccaatgtgga attggcaaaa agtatgacag
      721 aattaataat agcccaaagg gcctattcat ttaatgctaa gtccttggaa aacacagatg
      781 aaatcatgtc cttgattaat aatattaaaa ggtaagctgc tataacataa agccttccca
      841 atgaatataa tggtaaaagt aaataggcgc atccacttac atgaatgcag ccttagcaga
      901 aatagcaaca ctggagggta caaaatttat gaaaccaata atcggtataa caagtaggga
      961 cgattacaca ggaagaatca aaataaacct agttaaccat acctatatag atgcagtaat
     1021 agaggccggt ggcatacctc tggtactagc taatttacaa gacttagatg atatagatgg
     1081 ctatataaaa accttagatg gcataatatt tagtggagga gaagacatct cccctctagt
     1141 atatggagaa aacccaataa aggaagttaa aaacatttcc tacagaaggg ataagtttga
     1201 attggagtta tttaaccgag cctatggaaa ggggttacct attctaggta tatgtagggg
     1261 aatccagcta gtgaatgtgg ccttaggtgg tagcctttac caggatatcc accagcaaat
     1321 acctcaggcc ttaggccatg tatctaccta taggatagaa ggcggatatc atactataga
     1381 tataaaaagg gattccatcc tttttgatat ctttaaggag aaaaagatac aagttaactc
     1441 ccaacaccac caaagtgtaa aagacttggg ggacaactta agagtcagtg ccagggctca
     1501 agatggagta atagaagcaa ttgagtctag caacgataac tttgtcctag gagttcaatt
     1561 tcaccctgag gccatggtaa gtgagcataa agaatttgta tgtatctttg aatattttat
     1621 taaccaatgc aaataaaaaa tccagactta agtctggatt ttttatttat ctgccaattc
     1681 aataaacctt tctatatctt tttcaattaa tctgagggaa tttctaaaga aatctggatt
     1741 atgcacatct atccccatcc ttaggcctac atccctaata ttgtttttgc ctgtggcatt
     1801 taacaagtca tcatactctt taataaactc ctgcccccta tttaaatatt cggcatatag
     1861 gcccttagaa aataagaggc caaaggcgta ggggaaatta tagaaattat tacctgctga
     1921 gtaataatgg gatttattta accacatata agggtgaagg aaatcatgat ctagtccatt
     1981 tccataggcc tgtttctggg catctatcat aatttctttt agttcattta cagataggga
     2041 gtagtttttt cttctattaa atagttctgt ttcaaacaga tatctactat atatatcaac
     2101 tattacctgg cctgcatcag atatggatgc ctccaggata cttaaagcct cctgttggtc
     2161 agcctccctt aaggctgcat tggtgactat ggtttcgcag aaaatagagg cagtttctgc
     2221 tataggcata ggatatctac tatttaagat tgattcgtcc tttaaattaa agccatgata
     2281 tgcatggcct aactcatggg ccaaagtgat catattggaa aagctgccat caaagtttgc
     2341 taaaatccta ctttcaccaa taggatgtag attggcacaa aaagctcctc ctaccttgcc
     2401 ctccctgggc tccacatcta accacttatt tctataggcc ttatccacaa agttggccaa
     2461 cctatcacta aaactcctaa agttatccac aatgtattcc atggcttcat catagccaaa
     2521 ggtcatatta acctggccta caggggcaaa taagtcataa aagggcaatc cattcttatg
     2581 gcctaaaatt tctgcctttt tcctataata cttatgaaag actggtaaaa actcttccat
     2641 agcagtaagc atggcatcta agctttcctt atccattcta gacttaaaca aggtttcctc
     2701 caaaggagac tcaaaacctc ttagctcaga ggtggttaaa acctcaccct taatactatt
     2761 tagggcagct gctgaagatt cctccacctt tttataggcc ttaagctctg cttcataggc
     2821 taactttcta atatttgggt ccttatcata ggccatattt ctaactacag ataggggcag
     2881 ttgcttatcc tctccatcta tataaatatc cactatcaag gtagatgtaa gcctgtcttg
     2941 taaattggac caggctgtag atccggtatt ggataattta gatataagga tttcttcctt
     3001 ttcacttagc aagtacctac tcctatcttt aatctgtctt aaatagaagc tatgctcctt
     3061 taaaaggtca gagctagata ttaatccatc taagttatca atttctttga taaagttttg
     3121 aaattggact tctggcttgg taatttctgt agattttatc ctaagcctat ctaaatactg
     3181 aatagcctct tcatccctag catcaacagt aatttttaaa ttggcaaagg acattaatct
     3241 agtaaataaa ctggacaggg tttgtgaata gttaatataa ctttcaattt tttcacctgc
     3301 cccatttagg gaagataaat tttcatcagc ccatacttga atttctttaa tcaattgatc
     3361 tagcttcctt aaatcccttt tatattcctc agaatcaaag gaaggatata agctgtctaa
     3421 actccacctc atctaaccac acctttcaga aaatatatat attgattgta tcataatggg
     3481 ccttaggact ctattaatat gttttcccgc atagggatat ggtttaattt tacatatttg
     3541 taaaataata aatattttca ttgcaataaa accatattaa gtatatactc ttatagttat
     3601 gtaattaagg aggataagta ttgaaaaaat taaactttaa tcaaatagat ttaaaggacg
     3661 aaatcctaag agggatttca gatatgggtt ttgaggaaat gtctcccatt caagaacagg
     3721 caattccaaa aatattaaaa gggcaagaca tcataggtca ggcccaaact ggaacgggaa
     3781 agactgcagc ctttggtatt cccctactgg aaatgtgtga tccaaataat aggagcttgc
     3841 aggccttaat cctgtgcccc accagagagt tatctatcca ggtttcagaa gaaattagaa
     3901 ggataggtaa gtataccagg ggaatcagta ccctgccagt ttacggtggc caaccaatag
     3961 ataggcaaat tagggccttg aaaagaggag tacaaattgt ggtaggtacc ccaggcaggg
     4021 taatagacca tataagaaga aggaccctaa gattggatca gttaaaaatg atggtgcttg
     4081 atgaggcaga tgaaatgttt gatatgggct ttagggatga tataggcctg gttatggata
     4141 aactgccaga ggaaagacaa accatattct tttctgccac catgccaaaa gaaattataa
     4201 actttgcatc aagataccaa aaggatcctg agatagtcag ggtagtccac aaagagctga
     4261 ccatgcctaa aatagagcag tattattttg agctaaagga acatatgaaa acggaaatat
     4321 taagtaggct tatagacata tataacccta gattgtccat agtcttttgt aatacaaaga
     4381 ggaaagtcga tgaattaaca gaaaagttgg tggctagagg ttatttggca gatgggcttc
     4441 acggagattt aagacaacct caacgggata ctgtaatgaa taaatttaga agaggcagta
     4501 tagatatcct agtagccaca gatgtggcgg ccagaggttt agatgtggat gatatagatt
     4561 tggtaataaa ctatgatatt ccccaggacg aagagtatta tgttcacagg attggtagaa
     4621 ctgccagggc cggtagggaa ggtattgcct ttagttttgt agtggccagg gataaaaaca
     4681 aacttagaaa tattcaaaaa tacgccaaaa ccactatcag acgaaaggat ttaccaacct
     4741 taaaggatat ggaagaagtt tacagatcca gcctgttaga caatataaaa gaagagataa
     4801 gtaagggcaa attaggcaaa tacaataaaa ttgtagatct attaatagaa gaaggctata
     4861 cctcctttga tatagctgca gccctattaa agttccatat ggaggcaaat aagtataata
     4921 aacatgaaga gttagacatg gtagattatg ggcagccact taaatctaca gccatggcca
     4981 gactatatat aaatataggt agtagaaagg gagccagccc tagacatatt ctagcagcca
     5041 tagtccagga gacaggtatt aagagaacaa aagtaggtac catagatgtg tatgataagt
     5101 tttcatttat tgaagtacca gctgatcagg ctaaatttat tatgaataga ctaaacaaaa
     5161 aaagaataag aggtagccgg gtaagagttg aaatggctaa tccaaaaaga aaatagtaaa
     5221 aactattttc tttttttctt tagcacactt ctaagaccaa tttcttcttt tctataaatt
     5281 ctttccaggt taggcaggtg cttatagaca cttaaaagtg ctataaagat tgctaagatt
     5341 aaagagccag ttccatattt aaagtaaatg gtagcaagaa taaagaagct aacta
//
LOCUS       DATGSI010000092         1604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_574198, whole genome shotgun sequence.
ACCESSION   DATGSI010000092 DATGSI010000000
VERSION     DATGSI010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574198"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VK031_01380"
     CDS             <1..910
                     /locus_tag="VK031_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004818020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLS52599.1"
                     /translation="KKEDDYYLVKTKEGNEYKTKYVVNAAGVYGDVIANLVNPNSHEI
                     IERHGSIMIIDGNIGFELQSTLFPVPGAHTKGMAAIPACGGNIILGSTAEIQKDKEDT
                     SFTKGQADHLFNSASRLVPELEKKHIIRVFAGLRPVEVNSDNDFVIEEDKDNEGFYNV
                     IGIQSPGIASSFAIAEYTVNMIKDGKDFKEKKDYDKTRKPIIDFSELSTEDRKKLVEK
                     DPKYANVICRCEIVPEAEILDAIRRPCGAKTVDGVKRRTRSGMGRCQGGFCESRIVEI
                     LAKELGKKPQEILKENKGSEILIGEE"
     gene            912..>1604
                     /locus_tag="VK031_01385"
     CDS             912..>1604
                     /locus_tag="VK031_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004817866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLS52600.1"
                     /translation="MVYDLIIIGGGPAGLTAAKKAYEEGIRNMLIIERDKELGGILNQ
                     CIHNGFGILKFNEELTGPEYAGRYIDFVKEYDINYLLHSIVLDIKEIENGNKIVECAS
                     MDDGYLELETKTVILAMGARERTRMMIETPGLRPAGVFNAGLAQKYLNIDGYKVGEKV
                     VILGSGDIGLIMARRLTLTGAKVERVVEIEPYTNGLQRNVVQCLDDFDIPLQLSHTIA
                     EIRGKDRVESVVI"
BASE COUNT          629 a          216 c          344 g          415 t
ORIGIN      
        1 taaaaaagaa gacgactact acctagttaa aactaaagaa ggtaatgaat acaaaactaa
       61 atatgtagta aatgctgctg gagtatatgg ggatgttata gcaaacttag taaatcctaa
      121 ttcacatgaa ataatcgaaa gacacggctc tattatgatt attgatggaa atataggctt
      181 tgaacttcag tcaacacttt tcccagtgcc aggagcacat acaaagggaa tggcagccat
      241 accagcttgt ggtggaaata taatacttgg ttcaacagca gagatacaaa aagataagga
      301 agatactagc tttactaaag gccaagcaga ccacttattt aattcagcat caaggcttgt
      361 acctgaactt gagaaaaaac atataataag agtttttgca ggtttaaggc cagtagaggt
      421 aaactctgac aatgactttg ttatagaaga agacaaagac aatgaaggat tttataatgt
      481 aattggaatc caatcaccag gaattgcatc atcatttgct atagctgaat atacagttaa
      541 tatgatcaaa gacggtaaag atttcaaaga aaagaaagac tatgataaga ctagaaagcc
      601 aattatcgat ttttctgaac tatcaactga ggataggaaa aaactagttg aaaaagatcc
      661 taaatatgca aatgtaatat gtaggtgtga gatagtacct gaagcagaaa tattagatgc
      721 tataagaaga ccttgtggtg ccaaaacagt agatggagtt aaaagaagga caagatctgg
      781 aatgggtagg tgccaaggag gattctgtga atcaagaatt gttgaaatac tagcaaaaga
      841 acttggtaaa aagcctcaag agatattaaa agaaaacaag ggcagcgaaa ttttaattgg
      901 agaagaataa tatggtttat gatttaataa ttataggtgg aggtcctgca ggacttacag
      961 cagctaagaa ggcttacgaa gaaggcataa gaaatatgct tataatagaa agagacaagg
     1021 agctaggtgg tatattaaac caatgtatcc acaatggttt tggtattttg aaatttaatg
     1081 aagagctaac aggaccagaa tatgctggaa gatatataga ctttgttaaa gaatacgata
     1141 tcaactacct cctacactct atagttttag atattaaaga aatagaaaac ggtaataaga
     1201 tagttgaatg tgcatctatg gatgatggat acttagagct tgaaacaaag actgtaattc
     1261 ttgctatggg tgctagagaa agaacaagga tgatgattga aactccagga ttaaggccag
     1321 caggagtatt caatgcagga cttgcccaaa aatatttaaa catagatgga tataaggttg
     1381 gagaaaaagt tgtaatacta ggttcaggag atataggcct aataatggca agaagactta
     1441 ccctaacagg ggccaaggta gaaagagttg tagaaattga accttatact aatggtcttc
     1501 aaagaaatgt tgtccaatgt ttagacgact ttgatatccc tcttcaactt tcccatacca
     1561 tagcagagat aagaggaaaa gacagggttg agtcagttgt aata
//
LOCUS       DATGSI010000093         1719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_385101, whole genome shotgun sequence.
ACCESSION   DATGSI010000093 DATGSI010000000
VERSION     DATGSI010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385101"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /gene="pheS"
                     /locus_tag="VK031_01390"
     CDS             <1..371
                     /gene="pheS"
                     /locus_tag="VK031_01390"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397864.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HLS52601.1"
                     /translation="NVSMANLIHTLDIFIKELFGAGMKTRYRPHHFPFTEPSAEVDVS
                     CISCMGQGCEQCNYTGWDMELLGCGMVHPQVLENCGIDSEKYTGFAFGMGIDRITMVK
                     YGINDIRLLFENDNRFLQQF"
     gene            392..>1719
                     /gene="pheT"
                     /locus_tag="VK031_01395"
     CDS             392..>1719
                     /gene="pheT"
                     /locus_tag="VK031_01395"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HLS52602.1"
                     /translation="MLLPVNWLKDYIDTDKTSRELADGITLSGSHVESIYSMDQGVEK
                     VVVGRIDKIEKHPDADKLLVCKVDVGTDVLQIVTGAHNIKEGDYVPVALVGAKLPAGM
                     EIKETALRGVDSFGMLCSMDELGFADNVLSKEMKEGIFILDKKYPLGTCIKEILGLNN
                     EVMELEITPNRPDCLSIIGMARETSATFGVALKEPEIQIKKEEEDIRDYLDDIVVESP
                     NCSRYYARVVKDVQIKESPLWLQLRLMEAGVRPINNMVDITNYVMLEYGEPLHAFDLE
                     KINGRKIIVRQAKEGEKIKTLDQVERKLDEKDLVIADEKEAIAIAGVMGGLDSEITED
                     TNLVLLEGANFNERSVRLTSKRLGLRTEASIRFEKGLDPNLCQTAVNRVCQLIEELGA
                     GVVIKSEIDVYKEKKEEKIIELRPDRANKLLGLNLTIEAMIKYLNALGLKS"
BASE COUNT          627 a          245 c          407 g          440 t
ORIGIN      
        1 aaaatgtatc catggcaaat ttaatccaca ccttagatat atttataaaa gaattgtttg
       61 gagcagggat gaaaactaga tataggccac accactttcc ctttacagag cctagtgcag
      121 aagtagatgt atcctgtata agctgtatgg gtcaaggctg tgagcaatgt aattatacag
      181 gctgggatat ggaactatta ggatgcggta tggttcatcc acaagtattg gaaaactgtg
      241 gtatagattc agaaaaatat actggatttg cctttggcat gggaatagac aggataacca
      301 tggtcaagta tggtataaat gatatccgtt tgttatttga aaatgataat agattcttgc
      361 aacaattcta ataggggaaa ggaaggataa gatgttatta cctgtaaatt ggttaaaaga
      421 ctatatagat acagacaaga caagcaggga attggcagat ggaatcacct tatctggttc
      481 acatgtagag tctatctact caatggatca gggagtagaa aaggtggtag taggaaggat
      541 agacaaaatt gaaaaacatc cagatgctga taaactgctg gtttgcaagg tagatgtagg
      601 aacagatgtg ttacaaatag ttaccggagc ccataatatt aaggaaggtg actatgtacc
      661 agttgcccta gttggagcca aactaccagc aggtatggaa ataaaagaga ctgccctgag
      721 aggagtagac tcctttggaa tgttatgctc tatggatgaa ttaggctttg ctgacaatgt
      781 attatctaag gagatgaagg aagggatctt tatcctggat aagaaatatc ccttaggtac
      841 atgtattaag gaaattttag gcctaaacaa cgaggtaatg gagctggaga taactcccaa
      901 taggccagat tgtctcagta tcattggcat ggccagagaa acatcagcca cctttggtgt
      961 tgccttaaaa gagccagaaa ttcaaataaa aaaagaagag gaagatataa gggactactt
     1021 agatgatata gtggtggaaa gccctaattg tagccgttac tatgctagag ttgtaaagga
     1081 tgtgcaaatt aaagaatcac ctttatggct tcagttaaga cttatggaag caggagtaag
     1141 gcctataaac aatatggtag atattaccaa ctatgtaatg ttggaatacg gtgaaccctt
     1201 acacgccttt gatctggaga agataaatgg tagaaaaata atagtaagac aggctaaaga
     1261 gggagaaaaa ataaaaaccc tagaccaagt ggaaagaaaa ctagatgaaa aggatctggt
     1321 tatagctgat gaaaaagaag ccattgccat agcaggagta atgggaggat tagattctga
     1381 aattacagag gatacaaact tagttctttt agaaggggca aactttaatg aaagaagtgt
     1441 acgactaacc tctaaaaggc ttggtttaag gacagaagcc tccattaggt ttgaaaaggg
     1501 cctggatcca aacctatgtc agactgcagt aaacagggtc tgtcaactaa tagaagagct
     1561 tggtgctgga gtggtaatta agtcagaaat agatgtttat aaggaaaaaa aggaagaaaa
     1621 aataatagaa cttaggccag atagagccaa caaactattg gggctaaatt tgaccataga
     1681 ggctatgatt aaatacttaa atgccctagg cttaaaatc
//
LOCUS       DATGSI010000094         1228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_526639, whole genome shotgun sequence.
ACCESSION   DATGSI010000094 DATGSI010000000
VERSION     DATGSI010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526639"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..>1228
                     /locus_tag="VK031_01400"
     CDS             10..>1228
                     /locus_tag="VK031_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52603.1"
                     /translation="MQYILLTDDNKLWLWGFRRDLNHSGAVVANALTSIQYGYSYPFQ
                     QVIPDNNSGRSLPNGVNATDIKMLFVTWGTIAITTCDGAVWVLSQNDHIAQGNAATEW
                     VRIHKANSADAKGDPLSNVVATRGSAYALMALTADGKVYTWGKETRLGNGTAPSNRYY
                     AREMSLPNNANGTVKMIGATSYDKNNPSSLTTPSLDNGKHSYYILYQTAGKNYGELWA
                     LGNNEKKQLGNWSTSSSNSWVQPRYDSASGPVMNDIVWISPQEHDMLYPFINVINQEQ
                     YLFNWGAEAQQALGRGTHNVSIGSSEVDPGKPSTWSEGSNEDIMGVESGGHTTMIIRK
                     CSSTFGYLGHRVHGSMGDGEIANIGGVAEVLFNTYPLQLCGIPTIDATLKANRGGPYC
                     KNIPIQLDYAPLGG"
BASE COUNT          407 a          179 c          270 g          372 t
ORIGIN      
        1 tcagaaggcg tgcaatatat acttttaact gatgataata aactttggct atggggattt
       61 agaagagatt taaatcatag tggagcagta gttgctaatg ctttaacttc aatacaatat
      121 gggtatagtt atccttttca acaagtaata cctgataaca attctggaag gtcattgcct
      181 aatggtgtaa atgctactga tataaaaatg ctatttgtta cttggggaac cattgcgatt
      241 actacttgcg atggagctgt ttgggtttta tctcaaaatg accatattgc acaaggcaat
      301 gctgctactg aatgggttag aattcataaa gcaaatagtg cagatgcaaa aggagaccct
      361 ctctcaaatg tagttgcaac gagaggatct gcatatgctt taatggcttt aacagctgat
      421 ggtaaagtct atacttgggg taaagaaact agattaggaa atggaacagc acctagtaat
      481 agatattatg cacgagagat gagcctgcct aacaatgcca atggcacggt gaaaatgata
      541 ggagctacta gttatgataa gaataatcct agctcgctca cgacgccaag tttagataat
      601 gggaaacata gttattatat attatatcaa acagcaggta aaaattatgg agaactatgg
      661 gctttaggta ataatgaaaa aaaacaatta ggaaactgga gtactagctc tagtaattca
      721 tgggtacagc ctaggtatga tagtgcttct ggtcctgtaa tgaatgatat tgtttggatt
      781 tccccacaag aacatgatat gctttatcct tttataaatg ttataaatca agaacagtat
      841 ttatttaatt ggggagcaga agcacaacaa gctttgggaa gaggtacgca taatgtctca
      901 attggctctt cagaagtcga tcctggtaaa ccatctactt ggtcagaagg tagtaatgaa
      961 gatattatgg gagtggaatc tggtggccac acaactatga ttataagaaa atgttctagt
     1021 acttttggtt atttaggtca tagagtacat ggaagcatgg gtgatggaga gattgcaaat
     1081 attggaggtg tagctgaagt gttatttaat acttatcctt tacagctttg tggcattcct
     1141 actattgatg ctaccttaaa agcgaataga ggtggccctt attgtaaaaa tattcctatt
     1201 caattagatt atgcgccatt aggaggta
//
LOCUS       DATGSI010000095         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_288903, whole genome shotgun sequence.
ACCESSION   DATGSI010000095 DATGSI010000000
VERSION     DATGSI010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288903"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VK031_01405"
     CDS             <1..630
                     /locus_tag="VK031_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004839647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HLS52604.1"
                     /translation="NTPYIQRPLDLGADIVVHSATKYLNGHGDVIAGFAVGSAELMEQ
                     VRGVGIKDMTGAALSPFDAFLITRGMKTLELRMERHCANAQKVAEFLESHPAVESIYY
                     PGLESFPQYDLAKRQMKLPGAMIAFEVKGGLDAGRQLMNTVKLCTLAVSLGDAETLIQ
                     HPASMTHSPYTEEERAESGISEGLVRLSVGLEDHEDIIKDLKEALDKLT"
     gene            662..1714
                     /locus_tag="VK031_01410"
     CDS             662..1714
                     /locus_tag="VK031_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HLS52605.1"
                     /translation="MRRMDDGLGFVLRYENVAWYEDGLVKILDRRIYPTKVEFVTCRS
                     HKEVAQAIRDMVTQSAGPYTAAGMGMALAAYESRSMAKDEMWDYLKKAAHRLSHARPT
                     TANRMSQVTEGCLKVAKKAMERGQRVDEKIFEYTIESLNRRYSIMEKVGEYLVDMFPD
                     KGNIMTQCFGETIVGMMLRVAKRRNKDISLYVPETRPYFQGARLTASVAHDQGFDTTV
                     ITDNMPAVVMETKNIDLFTSAADIICLDGHITNKVGTYQIAIAAKYHGIPYFVTGIPD
                     RDHDDVSTVIIEERDPQYVLEARGVKNTLEGVKGYYPSFDITPPHLISGVVTDKGIFA
                     PYDLHRYFETDVVQFY"
     gene            1730..>2327
                     /locus_tag="VK031_01415"
     CDS             1730..>2327
                     /locus_tag="VK031_01415"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="related protein from Bacillus subtilis functions as
                     methylribose kinase and functions in salvage pathway;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HLS52606.1"
                     /translation="MADFNKHFRMKVEDVVLYAREKLDIFSEDADLKAKEIGDGNINY
                     VFKVWDEKTGQSVVIKQADTLLRSSGRPLDTDRNRIEAEALMLQSKLAPSFVPKVYKY
                     DPIMCALTMEDISDHYNLRYELLKRKKFPKFADHITSFMVNTLLLTTDLVLDPAEKKR
                     KVKNFINVELCDISEKLVFTEPYNDYLGRNIILEENLNF"
BASE COUNT          784 a          353 c          554 g          636 t
ORIGIN      
        1 aatactccat atatacaaag acccttagat ttaggagcag atattgtggt ccactcagca
       61 actaaatact taaatggaca tggggatgta atagcaggtt ttgcagtggg tagtgcagaa
      121 cttatggaac aagtaagggg agtaggaatt aaggatatga ctggagcagc cttaagtccc
      181 tttgatgctt ttctaataac caggggaatg aagacgttgg agttaaggat ggaaagacat
      241 tgtgccaatg ctcaaaaagt ggcagaattt ttggaaagtc acccagctgt tgaaagcatc
      301 tactatccag gcctagaaag ttttccccag tatgatctgg caaagagaca aatgaaatta
      361 cctggagcca tgattgcttt tgaggtaaag ggtggtctgg atgcaggtag gcagttaatg
      421 aatactgtaa agctttgtac tctggcagta agcctaggag atgcagaaac tttaattcag
      481 catcctgcca gcatgaccca ctcaccatat acagaggaag agagggctga aagcggcata
      541 tctgaagggc tagttagatt atcagtaggc ctggaggatc atgaagatat aattaaagat
      601 ttaaaagagg ctctagataa attaacataa atttagtata gatttacaaa ggagatggct
      661 tatgaggaga atggatgatg gattaggatt tgttttaaga tatgaaaatg tggcctggta
      721 tgaagacggc ctagttaaaa tattagatag aagaatatat cctaccaagg tagaatttgt
      781 aacctgcagg agccataaag aggtggccca ggccattagg gatatggtca cccaaagtgc
      841 tggtccctat actgcagccg gtatggggat ggccttggcc gcctatgaaa gtagatccat
      901 ggcaaaggat gagatgtggg actatttaaa aaaggcagcc cataggctat cccatgccag
      961 gcccactact gccaatagga tgagccaggt aactgaaggt tgcctaaagg ttgccaagaa
     1021 ggcaatggaa agaggtcaaa gggtagatga aaagatcttt gaatatacga tagagtcttt
     1081 gaatcggaga tattctatca tggaaaaggt gggagaatat ttggtagata tgtttcctga
     1141 taagggaaat ataatgaccc aatgctttgg tgagactata gttggaatga tgctaagggt
     1201 agccaaaagg agaaataagg atataagctt atatgtgcct gagaccaggc cctattttca
     1261 aggggccagg ttaacagcca gtgtggccca tgatcaagga tttgatacta ctgtaattac
     1321 agataatatg ccggctgttg taatggaaac aaaaaatata gatctattta cctcagcagc
     1381 tgacatcata tgtttagatg ggcatattac taacaaggta ggaacatacc aaatagccat
     1441 tgcagccaaa tatcatggaa tcccctattt cgtaacagga ataccagata gggatcatga
     1501 tgatgtttct acagtgatca ttgaggagag ggatcctcaa tatgtgctgg aggctagagg
     1561 tgtgaaaaat acattggaag gtgtcaaggg ttactaccct tcttttgata taactccacc
     1621 ccacttaatt agtggagttg taacagataa gggaatattt gctccctatg accttcatag
     1681 gtattttgaa actgatgtgg ttcaatttta ttaaaaagga aggtttaaaa tggcagactt
     1741 taataagcat tttaggatga aagttgaaga tgttgttctt tacgctaggg aaaagttaga
     1801 tatatttagt gaagatgctg atttaaaggc gaaagaaata ggtgacggta atattaatta
     1861 tgtatttaag gtctgggatg aaaagacagg ccagtcagtg gttataaagc aggctgacac
     1921 tctgcttaga tcatctggta ggcctttaga tacagataga aataggattg aagccgaagc
     1981 tttaatgctt caatctaaat tagcaccaag cttcgtacct aaggtttata agtatgatcc
     2041 tataatgtgc gccttaacaa tggaggatat ttcagaccat tataatttac gatatgaact
     2101 actaaagagg aaaaagtttc ctaaatttgc agaccatata acaagtttta tggtaaatac
     2161 cctacttttg actacagact tggttctaga tccggctgaa aagaagagaa aagttaaaaa
     2221 ttttattaat gtggagcttt gtgatatatc tgaaaagcta gtatttacag agccctataa
     2281 tgattatcta ggtagaaata ttatcctaga agaaaaccta aattttg
//
LOCUS       DATGSI010000096         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_336226, whole genome shotgun sequence.
ACCESSION   DATGSI010000096 DATGSI010000000
VERSION     DATGSI010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336226"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(509..>1170)
                     /locus_tag="VK031_01420"
     CDS             complement(509..>1170)
                     /locus_tag="VK031_01420"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLS52607.1"
                     /translation="LLPRLGAALTVVVSLTGQIIMGVTIDALGWFDAKLKASNLFNIL
                     GILLLIVGIFLMNYVKDRTKEKANIPFYLWMLAGFIIGFGPPIQTAINSKLGQEVNSS
                     FMAAFISFTVGFIILLLLKTIESRHFKLEIKDRTQGKLSPVYFIGGALGLVYITVNIF
                     LMPYLGAALTTVSGMLGQMILALVIDHFGLLGIRKRKVTSRKLVSIGIIIVGILFLRL
                     F"
BASE COUNT          392 a          197 c          223 g          358 t
ORIGIN      
        1 tgaataggaa tagccataaa agtgtaaaag atatagatag cctagaacaa gatatttttc
       61 aagaaatgat cgaatcgagg gaatcagtag atggtgaatt gatttttggc ttagcttagt
      121 tgaaatagct gatgaagatg aattcacatt tagaggaagt gttcgtcaaa ctcggaccta
      181 tacctaaatg aaattgatta agattataaa tttatttatt tccttatttg catgaaccgg
      241 ttcattaggg taataaagtt tataaactca atatttccaa tgaaagaaag ttggttctgt
      301 gctgggcttt agaatcagtg tgatttgtga taaaagtaaa tcattcgtca tcctgtagtt
      361 gtaggcttac aggtgaaata ttttataagt tatcattttg aaattagtag aaaagagcaa
      421 tcactgattt acaagttttt tatctcttaa tttatagaaa agcttcatta gactatccca
      481 gcgaactttc caacttcttc ctattctcct aaaataagcg taaaaataag atgccgacta
      541 ttataattcc tatagataca agttttcgtg aagtcacttt ccgtttacga attcctagta
      601 atccaaagtg atcgatgact agggctagga tcatttggcc taacatgccg gaaacagttg
      661 tcagggctgc gcctaaataa ggcattaaaa aaatatttac tgtaatatac acgagtccaa
      721 gcgctccgcc gataaagtag acggggctta actttccttg agttcgatct tttatttcta
      781 acttaaagtg tctactctct attgtcttaa ggagaagtag aatgataaag ccgaccgtga
      841 aggaaatgaa ggcagccata aaagaagagt ttacctcttg gcctagctta ctattaatag
      901 ctgtttggat aggtgggcca aagccaatga taaatccagc tagcatccat aaatagaagg
      961 gaatattcgc cttttcctta gtccgatctt ttacatagtt cattaagaag ataccaacaa
     1021 ttaataagag gatccctaat atgttaaaaa gatttgaggc tttaagcttg gcatcgaacc
     1081 aaccaagggc atcgatcgtt acccccataa taatctgacc cgttaaagag acgacaacag
     1141 ttagtgccgc ccctagtctt ggcagtaaga
//
LOCUS       DATGSI010000097         9613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_242374, whole genome shotgun sequence.
ACCESSION   DATGSI010000097 DATGSI010000000
VERSION     DATGSI010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242374"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VK031_01425"
     CDS             <1..406
                     /locus_tag="VK031_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52608.1"
                     /translation="KYYDYVVTDSNAFSGYIFGDIGLRDSVEEAVVRYKPFDQIISVL
                     LVPEGTDIKISEGLRKEKEINPSIVSRCKDVEIPHPYLFGERLKAERKVVTIRLLNDR
                     VNSSNIGRFSKSVWVTLDTFGYSGGGKASRRG"
     gene            complement(393..800)
                     /locus_tag="VK031_01430"
     CDS             complement(393..800)
                     /locus_tag="VK031_01430"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HLS52609.1"
                     /translation="MERFLKIDKDFLDVRELTLPDGEVVKVSPTDKLIHGHMYDRWKF
                     FTGSGSEYYDSLEDISKALAVHRNTVWRSVDKLNKLGLLAIEKKHIRGYESNVYTRVF
                     LVGEKFKVKKETPAVKASVPESPDTDLENLSPF"
     gene            complement(2754..3299)
                     /locus_tag="VK031_01435"
     CDS             complement(2754..3299)
                     /locus_tag="VK031_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52610.1"
                     /translation="MFKSLYNLLATNDGYFKSEKQARFLLSKLGNRVVQNMGFNLGSE
                     HNNKKVGNSAYRVFELDSKGVVSIVHHNAKGKEIIFERGGYNRYKAKQDRLNKSRKLV
                     TISKDLMEQINDLFLINFKETALKAGARPEHVNKVYPRLVKREITKSSTREIIDNPLF
                     SDLVKGLAEQWMDIEDKLRTM"
     gene            complement(3506..3856)
                     /locus_tag="VK031_01440"
     CDS             complement(3506..3856)
                     /locus_tag="VK031_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52611.1"
                     /translation="MLNTAKNLIKTYKNNISLMAQQAEKLCNDHSPFWSVETDVYYFS
                     DGSVLWINSYDEYGDTYLWFVFDLNNGIISDAYQCYDQAVDKLLEYVKSNPDNAVGFY
                     GIVDLFGNEEIYNG"
     gene            complement(4251..5807)
                     /locus_tag="VK031_01445"
     CDS             complement(4251..5807)
                     /locus_tag="VK031_01445"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HLS52612.1"
                     /translation="MLLAKNKSNEIKLIPSMLNRHGLIAGSSGSGKTVTLKKLAELFS
                     DAGIPVLVGDIKGDLTGFLTPIELTSGLKKRCENIGTAYPDPRSYPVRLWDVYNKKGK
                     QISTTIVDFGSTLLANVLQLNATQSGVLTIVFRVARDQDLALNTIDDLRQVLIYCLEH
                     RDNIGLYYGNITRSSISTIQRGLLTLEDQGGNYLFKDQDPFNFKTLIRKDKLNQGVIN
                     LLDCVELIKSPQLYGCFLIYLLSQFYDNLPEAGDLVKPEFIFFFDEAHLIFSNASKLL
                     LSELENIMRLIRSKGVGVYFCSQSPNDLPDIILGQLGHRIQHSMRAYTAAAQRDLKVA
                     ANTMRENPYINAEKEIPLLNIGEALISVLDSSGSPTITEKAYILPPNSKMGLADPNDY
                     HYSLDRALSHKLVSIHNDKNNNLDASQKTYEQENNNIADTGIDRLENIGSDSYEAFSG
                     DSRGFTSKPIGKYRGAALNALGGLTGGLKRIIGVVARILFILMIIAMCINLFPPLLFL
                     IPVLWGKIFE"
     gene            complement(5810..6223)
                     /locus_tag="VK031_01450"
     CDS             complement(5810..6223)
                     /locus_tag="VK031_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52613.1"
                     /translation="MTLERTFINDFIDACLDDGKNYLQEELCSLGYLPFSDCSIEKIE
                     SIAKTLFDYVAPVLRDNFKPWDLRSAGGDLYIAMMYDDLGTIYQKEVWKKLTEKDNPK
                     NVFGLSLLWFTGDDHIDPDPEHWYVEINYFTESGE"
     gene            complement(6286..6642)
                     /locus_tag="VK031_01455"
     CDS             complement(6286..6642)
                     /locus_tag="VK031_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52614.1"
                     /translation="MFGIVETLTKHQFIARFKEVRPYQFSDKGLEIIFDYFEGYADSL
                     NRDIEFDPIAICCEFEEMDIDEFTLNLSNLKHIDVDIEDYDSFNKIIEAATDAGVIVA
                     GYCEDADTVITESYSW"
     gene            complement(6695..6877)
                     /locus_tag="VK031_01460"
     CDS             complement(6695..6877)
                     /locus_tag="VK031_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52615.1"
                     /translation="MENIFYGLDWEILFYKKGKKDPVYVHRYRGGRRSAIELLKDYRQ
                     CYATAHDKIILKRKQV"
     gene            complement(7910..8185)
                     /locus_tag="VK031_01465"
     CDS             complement(7910..8185)
                     /locus_tag="VK031_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52616.1"
                     /translation="MKRIDVGDRVMYFYSFPCRYMREYEDYMPPDMLSGHMFIVMEKL
                     TGYLLHIEPECPSLKEIGGVLVDISNLVLIRKGHVRPILRLPNKRDR"
     gene            complement(8440..8829)
                     /locus_tag="VK031_01470"
     CDS             complement(8440..8829)
                     /locus_tag="VK031_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52617.1"
                     /translation="MNLIREEVFKHYISLMEQSWVVWCCKHTDDPIFDEAMKGYMRDV
                     LDSFYVKWESDGDSVVAKVSSPDGRDLGSVDFGEYSDDIKRLFSEVKNYHLFKGARDL
                     FLAFFVSQMKEMGALFWPPNLKTFKAA"
     gene            complement(8826..9089)
                     /locus_tag="VK031_01475"
     CDS             complement(8826..9089)
                     /locus_tag="VK031_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HLS52618.1"
                     /translation="MTYNDNKGMVVVVSKNHCPNCTVVKTVFDDWENIPYEEINIEFE
                     ENLDLLLALKEKGFQTLPIVIWKDKIYTHGEINEFLKDYKGTL"
     gene            complement(9095..>9613)
                     /locus_tag="VK031_01480"
     CDS             complement(9095..>9613)
                     /locus_tag="VK031_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52619.1"
                     /translation="PTTEGYDIVWTMWKHIAARNGARGVLRTSLNIAEQLHQHFAYEV
                     LRIMRDVEKYPEWEECLPKMKEIVDTVLEQELAWNEYLFEGKQMLGLNKGILEKAVKF
                     FALPVYDKLNIEAPYNTEEASINPIEHLLGYFDTSLIQTASQEIQQTGYITGSLYDDG
                     WRDADLSFKVGE"
BASE COUNT         2950 a         2045 c         1510 g         3108 t
ORIGIN      
        1 taagtattac gactacgtag ttacagactc taacgctttt agtggttaca tttttgggga
       61 catagggttg agagactccg tagaggaggc agttgttagg tataaacctt ttgaccaaat
      121 aatctccgta ctactagttc ctgaaggtac agatattaaa ataagtgaag gcctacgtaa
      181 ggagaaggag ataaacccct ctattgtgtc taggtgcaaa gacgtagaga ttccccaccc
      241 ctacttgttc ggagagaggc tgaaggctga gaggaaggta gtcaccataa ggcttctaaa
      301 cgacagggtc aactctagta acataggcag gttctccaaa agtgtttggg tcactctaga
      361 tactttcgga tatagtgggg gggggaaggc ctctagaagg ggctgaggtt ttctaaatct
      421 gtatcagggc tttctgggac acttgctttc acggcaggtg tctctttttt aaccttaaac
      481 ttttccccca ctaaaaacac acgagtgtac acattgctct catacccacg tatgtgcttc
      541 ttttctatgg ctagaaggcc cagcttgttc agcttgtcaa cacttctcca cacagtgttt
      601 ctatgcactg cgagagcctt agaaatatct tctagactgt cgtagtattc agagccagag
      661 ccagtgaaga atttccatct gtcgtacatg tgtccgtgta ttaatttatc tgtagggctc
      721 accttcacca cctccccatc aggaagggtg agctccctca catctaaaaa gtccttatca
      781 atctttaaaa atctttccat agtttccccc acagttttaa aaaatatacc acttatcggg
      841 ggataagtca agaagttatc accaaaatgg tgaagacgat gcgtaccata tttgtgatat
      901 agtaagactg aataataaga ctgatcatat atatatatat atatatatac atatggagaa
      961 tttgatgcct attagggggc atctttttta tgtctgaaat atctggggca ataaacatct
     1021 tactcctata atctcagaca ataaaatatc tttcttacag caagtagaca gattatgccc
     1081 ccatataggg aacataggca tatatgtgcc acatatattc tcgatcatac aataaacatg
     1141 aaagagagaa taaacaaata tatgttccca catagggcat ctattagtga ataggagagc
     1201 accactaata agagccttgt aagggtgctc tctatagggg gaatgctatt tgtcactctt
     1261 agagaaacat tcccccctta tattttattt attttatttt taatgatcga tcatacttat
     1321 aaaataaaaa tatctataat taaaatatct ggactatgca caccaacaca tatgtgtgct
     1381 tctatagtta tctacttggt tggtgtgtat cctcaaataa gggggaagcc cttatgagtg
     1441 cccatctggc ctttaggctt cccctatagg gatatatctt tcacataggt catagcatag
     1501 gaggctctgt aaagaagcta catatgttgt gcacatatgc agagcctata gggctacata
     1561 tgaaaacatt tgagttatat ttgcgtgctt acatagggct ctatttgtgg gcatatctgt
     1621 gctatatatg tgcatatgtg tgtcatacgt ggaacatatg tgtgtgccac atatttacat
     1681 atttagagca aatgtgttaa gcacgaatat ttccccaaat ttcccaatct gtcataattt
     1741 taaaatatct tacataatat tttatatcat tgcacagatt tgtaaccaag tgaaacattc
     1801 taatttgtca atccctattg tgtaacagaa tgtttatatt gtaacattat gtatttcccc
     1861 ttgactttgc cccctatctg tgctacctat tcaaggggga tatgtaacaa gatatttcct
     1921 tatgtgacta tctgtaacaa atctatttgt caagatttat tttagttaat ttgtaacaga
     1981 aagtaaatct acttgacaag ataattttat tatggtttga ataggccttg aatcttgccc
     2041 cctatctgtg ctaaaagtca aggcctagat tgtaacaaga tgttaaatgt aaccagatgt
     2101 aaaacatctt gctttatttg ctaatctatg attcagataa ggggctaaca tattgcccca
     2161 tatttgtcaa ccacatattt gtaacaagaa gtaaaatttg taacagatgg taaaatgggt
     2221 tgacaagagg ggaaaagtgt gtttaacaca acacactata cttatactca cacataacaa
     2281 aacaagaaac aagccctata tagcccacat atatgcccca ccatatgcca tatatttccc
     2341 ctatagtctt tatagaccac cataggccta caacactata tttgacaaat ttgccccaat
     2401 atggtaggcc tatcatattt cccctaagcc ttgttttaag cgctttacag ggctttacac
     2461 cttaagacta tgccccaata tgccccgcat atgataaagc ccttataagc cttataaaca
     2521 ccttataacc ctatatttcc cacctatgtt ttaccagcat ttccctatat tttaccaata
     2581 ggttcaatcc gatttaagcg catttaagcg ctttaaattg attaccctat actaccctat
     2641 acataaagca caaaaagcct tgtaaagggc tgtaaaagat tttaaaagct atagacaaaa
     2701 aataaggcca gcttaatgct ggcctagggt attgtatttg ttttattgat gtcttacatc
     2761 gttcttagct tgtcttcgat atccatccac tgttctgcta aacccttaac cagatctgaa
     2821 aacaggggat tgtctataat ctctcttgtg ctgctttttg taatttctct ttttactagt
     2881 cttggataca ctttatttac atgctcaggt ctcgctcccg ctttcaatgc tgtttccttg
     2941 aaattgatta gaaacagatc gtttatctgt tccattaaat ccttgctgat agtaactaat
     3001 ttcctagatt tattgagcct atcttgcttg gctttatagc gattgtagcc gccgcgctca
     3061 aagataattt cctttccttt agcgttatga tgaacaatgc taactacacc tttagagtct
     3121 agctcaaaca ctctgtaagc actgttacct actttcttat tattgtgctc actacccaga
     3181 ttaaagccca tattctgtac tactcgattg ccaagcttgc taagcagaaa tctggcttgt
     3241 ttttcacttt taaaataacc atcgtttgtt gctaacagat tgtagagtga cttaaacata
     3301 ttttatcccc ttaaatgatt agtgttgata cttattatta tacatattat tttatttaag
     3361 tcaacaagaa aaacaccaat agccagcgta ttgctggcta taggaggttt attaagttta
     3421 attattttat aaaaagcaag ctgtaataat cacagcaaag aataatattg ctcctaccag
     3481 atccgctaga acaaccttta aacgcttatc cattatagat ctcctcattg ccaaataaat
     3541 ctactatgcc gtaaaaccct acagcgttat ctggattgct tttaacatac tctaatagct
     3601 tatcaacagc ttgatcatag cactgataag catcactaat gatgccgtta tttaaatcaa
     3661 acacaaacca cagatatgta tcaccatatt catcataact attgatccac agtacgctac
     3721 cgtcactaaa ataataaaca tctgtttcta cagaccaaaa aggactatga tcattacata
     3781 gtttttccgc ttgttgagcc attaaactta tattgttttt gtatgttttt ataagattct
     3841 tagctgtatt aagcattgta gtcctcccag tccagtttga attcatttgc aaagatccta
     3901 acacaatcgc ataaaaggat ctcttgataa cattctgtac aataatctgt aaagccctca
     3961 tccgagtcat agaatgactc aatcaagctg aaatcaatgc ctttaaacat tatagatctc
     4021 caatcattag ttagtgttta gctgttgttt gatgctatgc cttaattata gcacagcatc
     4081 atattatttc aacctttaca ttatatagtc ttctaactct gctaaaaggc cgctttgtag
     4141 atattcatcg tattaccctt attaattaag tgaatgagtc ttaaatataa ccctgccctt
     4201 atccttatgc aacccttaaa gtgtaacaag gtgtaacact ggatgccctg ctactcgaag
     4261 atctttcccc atagcacagg aattaaaaaa agtaaaggcg ggaaaaggtt aatacacatg
     4321 gcgatgatca tcagaataaa taagatcctt gctactaccc caattatcct ttttaaacct
     4381 cctgtgagac ctcctaaggc gtttaatgct gcacccctat acttgcctat aggcttacta
     4441 gtaaaacccc tagaatcgcc tgaaaacgcc tcatagctat cagatcctat attttctaat
     4501 ctgtcaatgc ctgtatctgc tatattgttg ttttcttgct catatgtttt ttgacttgca
     4561 tctaaattat tgtttttgtc attatgtatg cttactaatt tgtgagacaa agcccgatct
     4621 aaagaataat gatagtcgtt cggatctgct aagcccatct tgctatttgg tggcagtatg
     4681 taagcctttt ctgtgattgt tggacttccc gagctgtcca aaacactaat taaagcctcg
     4741 ccaatattta acagagggat ctctttctca gcattaatat aagggttttc acgcattgta
     4801 tttgcagcta ctttcagatc tctttgagct gcagcggtat atgctcgcat actatgctgt
     4861 atcctatgcc ctaattgccc taagattata tcaggcagat cattaggtga ttggctacag
     4921 aaatacacgc ctacaccctt agatcttatc agtcgcatga tgttttctag ctcagataaa
     4981 agtagcttac tagcattaga aaagatgaga tgagcctcgt caaaaaagaa tataaattcg
     5041 ggttttacaa gatcccctgc ctctggcaag ttatcataaa attgactaag caaatagatt
     5101 aaaaagcagc cgtaaagctg cgggcttttt ataagctcga cacagtctaa taagttgata
     5161 acaccttgat ttaacttatc ttttcttatt agtgttttaa aattgaaagg atcttgatct
     5221 ttaaaaagat agtttccccc ttggtcttcc aaagttaata aaccccgttg aatcgtgctt
     5281 atgctggatc gtgttatgtt gccataatac aaccctatgt tatccctgtg ctctaaacag
     5341 tagattagca cttgacgcag atcgtcaatt gtgttaagag ctagatcctg atctctagct
     5401 actcgaaaaa caatagtcag gacacctgat tgagtcgcat taagctgcag cacattagct
     5461 agcaaagtag atccaaaatc tactattgta gtgcttatct gttttccttt tttattatat
     5521 acgtcccata gtctcactgg ataagatcgg ggatcggggt atgctgtacc aatattttca
     5581 catcgttttt ttaaccctga tgttagttca atcggcgtta aaaaaccagt aagatcccct
     5641 tttatatctc caactaaaac gggtataccc gcatctgaaa aaagctctgc tagtttttta
     5701 agcgttactg ttttaccgct gcccgatgat ccagctatta acccgtgcct attaagcatc
     5761 gaaggtatta atttaatttc attgctttta ttttttgcca ataacataat tattcccccg
     5821 attctgtgaa ataatttatt tccacatacc agtgctcggg atctggatct atatggtcat
     5881 ccccagtaaa ccaaagtaaa cttaaaccaa aaacattttt agggttatcc ttttccgtta
     5941 attttttcca cacctctttt tggtatatag tgccaagatc atcatacatc atagctatat
     6001 acagatcccc acctgcagat ctgagatccc agggtttaaa attatccctt aacactggtg
     6061 caacgtagtc aaacagtgtt ttagctatag actctatttt ttcaatagaa caatcgctaa
     6121 agggtaaata acctaaactg cataactcct cctgtaggta gtttttcccg tcatccaaac
     6181 aagcatcaat aaaatcatta ataaatgttc tttctagtgt cattctaaga tctccaatca
     6241 taaaataata aataagtata ggcaggccgc caaagcggcc catatttacc agctatatga
     6301 ttctgttatt actgtgtctg catcctcgca ataaccagca acgataacgc ctgcatctgt
     6361 cgctgcttcg ataattttat taaaagaatc ataatcttca atatcaacat caatgtgctt
     6421 taaatttgat aagtttaacg taaattcatc aatgtccatc tcctcgaatt cgcagcatat
     6481 ggctatggga tcgaattcta tatctctgtt taagctatca gcataaccct cgaaataatc
     6541 aaaaataatc tctaagcctt tatcactgaa ttgataaggt cttacttctt taaagcgtgc
     6601 aataaattga tgcttagtga gtgtttcaac gattccaaac atattgatct ccaatcataa
     6661 atatatttat aagccaataa ataaataaaa ttatttacac ttgctttctt tttaatatta
     6721 ttttatcatg agccgttgca tagcattgtc tataatcttt taataattct atagcagatc
     6781 ttctccctcc tctgtatctg tgaacataaa caggatcttt tttgcccttt ttgtaaaata
     6841 atatttccca gtccaagccg taaaatatgt tttccatttt aagatctcca atcaattaat
     6901 tagttagtta atgtgctgcg atgtcttaac tatagcttta ctctaatatc cttgcaaccc
     6961 ctaaagtgta acagtgtgta acagtgtttt gagcgcataa ctttatacat acttatatag
     7021 atacacgtgc acgcacgaat aacacaaatt tcaggtaaag tcaaacaata tttttttttt
     7081 tactcctaga aaacacaata tgccggatta gtcaatcact gttacatagt tttacaaaat
     7141 aaatttgaca aaatgaaatt attatgatcg tggttttatt ttacagatca cagggaaaca
     7201 aataagtcaa gcgcagttac accatgtaac aaattaaatt tggctatttc caagatctat
     7261 gatatatggg cacctaccac atataagaaa taaagtcaat acattttaca ccttgttaca
     7321 tattggcctt gacttttaaa aaatcttcaa gtgtaactaa ataattccag atttaactga
     7381 aagtagccaa atataaaagt tgtaaccaaa gtataccaaa tgtaaccaga tgtaaaatct
     7441 ggccagaaaa tcgaaaattt tttttgcgcc cacttccccc tataatgccc tgtataatgt
     7501 gttgaataat gtcgctaata atgtcgccaa gaatctctgc cagatgtgcc acttacctct
     7561 tctccaaata tggtgggaaa agtatagtgt ccccaataat ctccgcccct atctgtggtc
     7621 accttttccc catccttttt aatatcccct aaacaacatt cctcctccaa tcttatgcat
     7681 taacaaaaag atttacccca ccccaaccac acacacccct catagtttcc caataacacc
     7741 cctaggaggc tctgagaggc tctaggagaa gagatctgcc acttccctat accctagcct
     7801 atgtataggc ctatctctcc tagaacagcc cctaatacaa agcccttttt caggatcgaa
     7861 atcgcctaaa aaacgttcat tatatataat aaaaaatatc aggagcctat tacctatccc
     7921 tcttattggg aagtctcaat atgggacgaa catgcccctt tcttatcaac actaggttag
     7981 atatgtccac caatactccc cctatttcct tcaaggaagg acattctggc tctatatgca
     8041 acaaataacc tgttagcttc tccatcacta taaacatatg gccagataac atatctgggg
     8101 gcatgtagtc ctcatactcc ctcatatacc tacaagggaa ggaataaaaa tacatcactc
     8161 tatcacctac atcaatcctt ttcatattat cctccaataa aaatagccca gaagggtgtc
     8221 tctattttga gactaccaca tctgggctta ggtgtcaaac tctatttgtc tttatcttgg
     8281 aaatctccaa aggtgaggta gtaaaagaag ttaagccatt gcatgttaaa cttcactact
     8341 tgtctgttcc actctgcaac agcagtgaga tgctctcgca tcttggggtg gtattgacca
     8401 tagtcaatca tatcctttct ccacaatata gtgtgcatat caagctgcct taaaagtttt
     8461 taaattagga ggccagaaaa gtgctcccat ctctttcatc tgagaaacaa agaaggctag
     8521 gaataagtct cttgcccctt taaacaggtg atagttttta acttcagaaa ataacctttt
     8581 tatgtcatca gaatactccc caaaatcaac actacctaaa tctctcccat ctggggagga
     8641 aaccttagct accacagagt ctccatcaga ttcccactta acatagaaag agtctagcac
     8701 atctctcatg tagcctttca tagcttcatc aaatatgggg tcgtcagtat gtttgcagca
     8761 ccacaccacc caactctgtt ccataagaga tatatagtgt ttaaatactt cctctcttat
     8821 taggttcata atgtcccctt gtaatccttt aaaaactcgt ttatctcacc atgagtatag
     8881 atcttatctt tccagataac aataggtagt gtttgaaaac ctttctcttt tagagctagg
     8941 agaagatcta ggttttcctc aaactcaata ttgatttcct cataggggat gttttcccaa
     9001 tcatcaaaca ctgttttaac aacagtgcaa ttagggcagt ggtttttaga gaccaccacc
     9061 accatccctt tattatcatt gtatgtcata ttctttactc tcccacttta aaagataaat
     9121 cagcatctct ccaaccatca tcatataagc tcccagtgat ataccctgtt tgctgaatct
     9181 cttggctggc agtttgaata agagatgtat caaagtaccc aagtaagtgc tcgatagggt
     9241 tgattgaggc ttcctctgtg ttgtaaggag cttcaatatt caacttatca tacacaggaa
     9301 gggcaaaaaa cttaacagct ttttctagaa tacccttatt gagccctaac atctgctttc
     9361 cttcaaacag atattcattc caagctaact cttgctcaag cactgtgtcc acaatttcct
     9421 tcatcttggg caagcactct tcccactctg ggtatttctc cacatctcgc ataattcgta
     9481 acacttcata ggcaaaatgt tgatgcaatt gctccgctat gttcaaggag gttcgcaaaa
     9541 cccccctcgc accattacgt gcagctatgt gtttccacat agtccagact atatcatatc
     9601 cctcagttgt agg
//
LOCUS       DATGSI010000098         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_288965, whole genome shotgun sequence.
ACCESSION   DATGSI010000098 DATGSI010000000
VERSION     DATGSI010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288965"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /gene="spoIIAB"
                     /locus_tag="VK031_01485"
     CDS             complement(<1..307)
                     /gene="spoIIAB"
                     /locus_tag="VK031_01485"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HLS52620.1"
                     /translation="MNKNYMKLEFLSRSNNESFSRVVVAAFAAQLDPTLEEISDIKTA
                     VSEAVTNSIIHGYEYKEGLITIEATIDKDLIEIIIKDEGKGIDNIEQAMEPFFTSKPD
                     "
     gene            complement(320..655)
                     /gene="spoIIAA"
                     /locus_tag="VK031_01490"
     CDS             complement(320..655)
                     /gene="spoIIAA"
                     /locus_tag="VK031_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HLS52621.1"
                     /translation="MYLIMETSGKNLIVKLKGELDHHTSEATRRKIDSSFIDKNLLNI
                     ILDLRELTFMDSSGIGLIMGRYKKVKDRQGNLIIVSDNRYVDRILNMSGLLKIIDKYK
                     TIDKAISKL"
     gene            complement(769..>1107)
                     /locus_tag="VK031_01495"
     CDS             complement(769..>1107)
                     /locus_tag="VK031_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HLS52622.1"
                     /translation="LVVGKPLAMMFLEKDATVTICHSKTKDLHKLTKEADLVVTGIGK
                     PAYFDDKYFNENSIVIDVGMEKDESGSMAGDVDYDKVYPKVKAITPVPGGIGSITTSI
                     LLDQVVKACK"
BASE COUNT          326 a          234 c          143 g          404 t
ORIGIN      
        1 agtctggttt tgaagtaaag aatggctcca ttgcctgttc tatattatct attcccttcc
       61 cctcatcttt tataataatc tctattaaat ccttatcaat tgtagcctca attgttatta
      121 atccttcctt atactcataa ccatgtataa tagaattggt aactgcttca gatacggctg
      181 ttttaatgtc agatatttcc tccaaggtcg gatccaattg ggcagcaaag gcagccacaa
      241 ctactctaga aaaagattcg ttatttgacc tacttaaaaa ttccaatttc atataatttt
      301 tattcacaat atcaccccac tatagtttag atatggcctt atcaatggtt ttatatttgt
      361 ctattatttt aagtaaacca gacatattta atatcctatc aacatatctg ttgtcactta
      421 caataataag atttccctgt ctatccttta ctttcttata tctgcccatt ataagtccta
      481 tgcctgagct gtccataaaa gttagctctc ttaagtctaa gataatattt aataagtttt
      541 tgtctataaa agaactatct attttccttc tggtggcttc agaagtatga tgatctaatt
      601 ccccctttaa cttaacaatt aaatttttcc ctgatgtttc cattattaaa tacaaaatac
      661 ccctcctcct tagaatgcta ctagagctat tctacagaag acctaattta tccttcagag
      721 aattttgact agttttgtaa tataaaagag aaaggacccg aaggtccttt atttacaggc
      781 ttttaccacc tggtctaaca atatactggt tgttatactg cctatacctc ctggcacagg
      841 tgttatggct tttaccttgg gatagacctt atcatagtcc acatccccag ccattgatcc
      901 tgattcatct ttctccatgc ctacatctat tactatggag ttttcattaa agtatttgtc
      961 atcaaaataa gcaggcttcc ctatgcctgt tacaaccaaa tctgcctctt tagtaagctt
     1021 atgtaaatcc ttagttttag aatggcaaat tgtaacagtg gcatcttttt ctagaaacat
     1081 cattgccaaa ggtttcccta caaccaa
//
LOCUS       DATGSI010000099         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_150058, whole genome shotgun sequence.
ACCESSION   DATGSI010000099 DATGSI010000000
VERSION     DATGSI010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150058"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VK031_01500"
     CDS             complement(<1..752)
                     /locus_tag="VK031_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLS52623.1"
                     /translation="MAYTLLLVDDERSIRETLKIILESAGYRVITAQDGFEALDYLHE
                     NMVDILITDLRMPVMGGIELMKKALEVDPYLETIFISAYADIKSAVKALKLGAFDYIE
                     KSFTTEELIFTVEKAIERKRLVEENTTLRRLLDGEYNCEGVIGKSKNMQELFYLVSRV
                     AGSKANILLSGESGVGKDVFAQLIHKKSSSSSGNFIPINCGAIPENLIESELFGHEKG
                     AFTGAIQRKKGKFELAHGGTLFLDEIGELPLQ"
     gene            complement(774..>1527)
                     /locus_tag="VK031_01505"
     CDS             complement(774..>1527)
                     /locus_tag="VK031_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLS52624.1"
                     /translation="LVKGCICIFRDTTEIEELQKQIAMAEKLAALGRITSGITHEIRN
                     PLLPIRNASQHLLENYQDQADGQMVKLLKIIQEESERLNRFLQQLVSLNRDTFFATGE
                     CDLLQVLEETLLLLNYGIVKNDIKLELDIGPKDIRIPLNEDNLKQIFLNLILNAIDAI
                     VLKEKEKDRKICIKVEKEMDYSIVEINDTGIGMTKDELAKAFDPFYTTKDDGTGIGLP
                     IIHNIVTSSGGKISMESKLDKGTQVTLLLPIA"
BASE COUNT          432 a          343 c          211 g          541 t
ORIGIN      
        1 atctgtagtg gtagttcacc tatttcatca aggaaaagag ttcctccatg ggccagctca
       61 aattttccct ttttcctttg aatggctcct gtaaaggccc ccttttcatg cccaaagagc
      121 tccgattcaa ttaagttttc aggtatagca ccacagttaa ttgggataaa attaccggaa
      181 ctactagagc tttttttatg tattaattgg gcaaatacat cctttcccac accactttcc
      241 cctgatagga gtatattggc cttactgcct gccacccggc ttaccaaata aaataattcc
      301 tgcatatttt tactcttacc aataacacct tcacaattat attccccatc taaaagcctt
      361 cttaaggtag tattttcttc aactagtctc ttcctttcaa tagctttctc cactgtaaaa
      421 attaattctt ctgttgtaaa ggatttttca atatagtcaa aggcacctaa ttttaaagcc
      481 ttcacagcag acttaatatc tgcataggct gaaataaata tggtttctaa atatggatct
      541 acttctaaag cctttttcat cagttcaatt ccgcccataa caggcatcct taaatcagta
      601 attaatatat ccaccatatt ttcatgtaag tagtccaagg cctcaaaacc gtcctgggca
      661 gttataaccc tatatcctgc actttctaaa ataattttta aggtttccct tatggatctt
      721 tcatcatcca ccaataataa ggtatatgcc atttatccct aactcctttc ctcctaagct
      781 ataggtagca aaagggtaac ttgagtcccc ttatctaact tactttccat ggatattttc
      841 cctccagagc tggtaactat attatgtatt ataggcaggc ctataccagt gccgtcatct
      901 ttggtggtat aaaatgggtc aaatgcttta gccagttcat ccttggtcat accaattcct
      961 gtgtcattta tttcaacaat agaatagtcc atttcctttt ccaccttaat acaaatcttt
     1021 ctatccttct ctttttcttt taaaactatt gcatctatgg catttaagat cagatttaaa
     1081 aatatttgct ttaggttatc ctcatttaat ggaatcctta tatccttcgg tccaatatct
     1141 agctctaatt taatatcatt ttttacaata ccatagttta ataaaagcaa ggtctcctct
     1201 aaaacctgca atagatcaca ctctcctgtt gcaaagaaag tgtccctgtt tagacttacc
     1261 aattgctgta gaaaacggtt gagcctttcg ctttcttctt gaataatttt taaaagtttc
     1321 accatttgcc catctgcctg atcctgataa ttttcaagta aatgttggct ggcatttcta
     1381 attggcaaaa gagggtttct aatttcatgg gtaatacctg aagtgatcct gcctaaggcg
     1441 gccagctttt cagccatggc aatttgtttt tgtagctctt ctatttcagt agtatcccta
     1501 aatatacata tacagccctt taccaat
//
LOCUS       DATGSI010000100         1078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_574950, whole genome shotgun sequence.
ACCESSION   DATGSI010000100 DATGSI010000000
VERSION     DATGSI010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574950"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VK031_01510"
     CDS             <1..1004
                     /locus_tag="VK031_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52625.1"
                     /translation="DTDVITIISKPPAERVRNNVMNYLRSLDKPVVTIFLGEKPKHHE
                     ENLYQAYTLEEAAKLSVQLMRNEDIKAFRTESRFGAEEVEGKIIKGYYSGGTLAEEAA
                     MLIKDALGLDKEREEEKGFMLKYNGFEIIDLGDDQYTQGRPHPMIDPAIRAEYLERAQ
                     EELPDVILFDIVLGYGAHDNMAAALAPSIKKLKEEAKRQGKELYFVTSLCGTDKDPQG
                     YDKQKEILEGLGVIVCETNNQAVEKSLALVGKTLKYYEKKVRPKEKVTEKIKVKVSNS
                     MKGLITRSPKIINIGLKSFAETLDSFPCEVVQYDWRPVAGGDVEMMKILDFLRNYQF"
BASE COUNT          422 a          145 c          250 g          261 t
ORIGIN      
        1 atgatacaga tgtaataaca attatttcaa aaccacctgc tgaaagagta aggaataatg
       61 taatgaatta cttgagatcc ttagataagc ctgtggttac tatattctta ggggaaaaac
      121 ctaaacatca tgaggaaaac ctatatcagg cctacacatt agaagaagca gcaaagctgt
      181 ctgtacagtt gatgagaaat gaagatataa aagcatttag gacagaaagt agatttgggg
      241 ctgaagaggt tgaagggaaa ataatcaagg gttattactc aggtgggacc ctagcagaag
      301 aggcagctat gctaattaag gatgccttag gcctagataa ggagcgagaa gaagaaaagg
      361 gatttatgct taaatataac ggattcgaaa taatagatct gggagatgat caatatacac
      421 aaggaaggcc tcacccaatg atagatccgg ccataagagc tgaatatttg gaaagggctc
      481 aagaggaatt accagacgtc atcttatttg acatagtatt aggttatggc gcccatgaca
      541 atatggcggc agcactggca ccaagtataa agaagttaaa agaagaagca aaaaggcagg
      601 gtaaagaact ctactttgta accagcttat gtggaacaga taaggaccca caaggctatg
      661 ataaacaaaa agaaattctg gaaggactcg gtgtgatagt ttgtgaaacc aataatcaag
      721 cagtagagaa atcgttagcc ttagttggta aaactctcaa atattatgaa aaaaaggtaa
      781 ggcctaagga aaaggttacc gaaaaaataa aggtaaaggt ttctaatagt atgaaggggt
      841 tgattactag aagtcctaaa ataataaata taggattaaa gagttttgct gagaccctag
      901 attccttccc ctgtgaagta gttcaatatg actggagacc agtagcaggt ggagatgtgg
      961 aaatgatgaa aatcttggat tttttaagga attatcaatt ctgataaaag aatatgaata
     1021 gggggaaata aaaaatgaaa tataaaacta tagatgatgc taacagggct gtaattaa
//
LOCUS       DATGSI010000101         1472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_54948, whole genome shotgun sequence.
ACCESSION   DATGSI010000101 DATGSI010000000
VERSION     DATGSI010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54948"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VK031_01515"
     CDS             complement(<1..569)
                     /locus_tag="VK031_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HLS52626.1"
                     /translation="MSEHNDRKRRNRRKANKKTSVNKSIKITLLVLLILIVMAGGTLA
                     GIVLSMVKAAPEIDPSQINLDLRHTSSILDLEGNLIEKIETAEFRTFVSINRMPEHLK
                     QAFIAIEDERFFDHRGVDPKGIVSSALENLKADGIVRGASTITQQLVKNVYLTPEKKW
                     SRKVQEAYLALQVDRALSKEQILEAYLNR"
     gene            819..>1472
                     /gene="hflX"
                     /locus_tag="VK031_01520"
     CDS             819..>1472
                     /gene="hflX"
                     /locus_tag="VK031_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HLS52627.1"
                     /translation="MDGDNLNLAKTSMEKVLTVGVDMDTDPINIEGSLDELEELVRAA
                     GGLSLGRVIQKRKAIDPAYFIGRGKAIEIKEYCDKLDIDTVVFNDELSGAQLRNLEEL
                     INKKILDRTNLILDIFANRASSKEGKLQVKLAQLKYRLPRLVGFRDYLSREGGGIGTR
                     GPGEQKLETDRRHILREINNIEKELRNIEANRDIKRKRRLDSNLPIVALVGYTNAGKS
                     "
BASE COUNT          492 a          220 c          282 g          478 t
ORIGIN      
        1 ttcctattta agtaagcttc caaaatttgt tccttgctta aggccctgtc cacctgtaag
       61 gctaggtaag cctcttgaac ctttcttgac cattttttct ctggggtcag gtatacattt
      121 ttgaccaatt gctgggttat agtactggcc cctctaacaa tgccatctgc ctttaagttt
      181 tctaaggctg agctgacaat tccctttgga tctacccccc gatggtcaaa aaacctttca
      241 tcttctatgg ctataaaagc ttgttttaaa tgttcaggca ttctatttat gctgacaaag
      301 gttctaaatt cagcagtttc tatcttttct attagattgc cttctaagtc tagaatagaa
      361 gatgtatgtc ttaaatctaa attgatctgg gatggatcaa tttctggtgc tgcctttacc
      421 atagataaaa ctatgccagc caaggtacca ccagccataa caataagtat tagtagcaca
      481 agcaaggtta ttttgatact cttgtttact gatgttttct tatttgcttt tcttctattt
      541 cttctttttc tatcgttatg ttcagacatt ttttcctcct taaattatca aaaggtatag
      601 aacaaataca ttataacata gttataatat attgttaata agttgaatat tatataatta
      661 ttacaaaaat aatggaaaag cctgttatat tagtttgtcc atatttatat tttttaaaac
      721 ttgtctgatt tatattaata agccttctca gagaaatctt agtatatatt agataatagg
      781 tataaagaat ggtataatta taataagcag aagcttaagt ggatggtgat aatttgaatt
      841 tagcaaagac aagtatggaa aaggtactga cagtaggggt agatatggat acagacccta
      901 ttaatattga aggctccctg gatgaattgg aggagttagt aagggccgcc ggtggacttt
      961 cactgggcag ggtgattcaa aaaagaaagg ccatagaccc agcctatttt ataggaagag
     1021 gtaaggcaat agagattaaa gagtattgtg ataaattaga tatagataca gtagtcttta
     1081 atgatgaatt atcaggagcc caattaagaa atttagaaga attgataaat aaaaaaatat
     1141 tagacagaac aaaccttatt ttagatattt ttgccaatag ggccagttca aaggaaggaa
     1201 aattacaggt aaaattggcc caattaaagt atcgcctgcc tagattagta ggatttagag
     1261 attacttgtc tagagaaggt ggaggtatag ggactagagg acctggagag caaaagcttg
     1321 aaacagatag aagacacatt ttacgtgaaa ttaataatat agaaaaagag ttaagaaata
     1381 ttgaggccaa tcgagatata aagagaaaga gacggcttga ttccaacctg cctatagtgg
     1441 ctttagtggg ctatacaaat gctggaaagt ct
//
LOCUS       DATGSI010000102         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_336638, whole genome shotgun sequence.
ACCESSION   DATGSI010000102 DATGSI010000000
VERSION     DATGSI010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336638"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VK031_01525"
     CDS             complement(<1..480)
                     /locus_tag="VK031_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HLS52628.1"
                     /translation="MSIKEGNMENIRGLLKKRKASSHKGDYGRVGIIAGSIGMAGAVN
                     LTSRAALRTGSGLVYSIVPESLELILSIKLTEAIIKPIGDKNIGYFTKDSLEQILKAT
                     KNMDALALGPGLGIDEERIYLVGELIKNINIPLVIDADGLNCLSKNINILKEVNYPII
                     "
     gene            781..>1096
                     /locus_tag="VK031_01530"
     CDS             781..>1096
                     /locus_tag="VK031_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004839002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HLS52629.1"
                     /translation="MYGQLIGNLSGFLYTYLLPLLLIGGGLYFSFKTGFVQLRLLGES
                     IRVVAEPSKDETAISSFQALMVSTASRVGTGNIVGVSTAIALGGAGAVFWMWVVAFVG
                     AAS"
BASE COUNT          307 a          188 c          178 g          423 t
ORIGIN      
        1 tataatggga taatttactt cctttaatat atttatattt tttgataaac aattcaaccc
       61 atctgcatct attaccaagg gaatatttat atttttaatt aattccccta ccaaatatat
      121 tctttcctca tctatcccaa ggcctggccc tagggctagc gcatccatat ttttagttgc
      181 ctttaatatc tgttctaagg aatctttggt aaaatagcct atgtttttat cccctatagg
      241 ctttataatt gcctctgtta atttaatgct taatattaat tctaaggatt caggcactat
      301 actatagacc aagccagatc ctgttcttag agccgcccta cttgtgaggt tcacagcacc
      361 tgccattcct atactgcctg ctattatgcc aacccttcca taatcacctt tatgagaact
      421 agcttttctt ttctttaata ggccccttat gttttccata ttgccttctt taatagacat
      481 ataaccatcc catttccttt gtttttagac aaataacctg aaaatcccta actacctgca
      541 taatatcata ttttaggaat aaatgtatat taataattaa tttatagaga aatttataaa
      601 agattgtgaa taatatagca acttctagct aagtgtgtta gtatgagggt aaatagccat
      661 gatatatata caactgtcac aattttaaaa ctatatataa gaaatggggt gatttataag
      721 tattaatagg gcctattatt tcgtctgcaa ggaaaatgtt ttatgtaagg agggaatctt
      781 atgtatggtc agcttattgg taatttaagt ggttttcttt atacttatct tttaccttta
      841 ctattaattg gtggtggact ttatttttct tttaaaactg gttttgttca gttaagactc
      901 ttaggtgagt ctattagagt tgttgctgaa ccttcaaaag atgaaactgc tatatcatct
      961 ttccaggctc ttatggtatc tacagcctct agggtaggta ctggtaatat tgtaggtgta
     1021 tctactgcta ttgccttagg tggtgctgga gctgtctttt ggatgtgggt tgtagctttt
     1081 gtaggtgctg catctg
//
LOCUS       DATGSI010000103         1223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_8543, whole genome shotgun sequence.
ACCESSION   DATGSI010000103 DATGSI010000000
VERSION     DATGSI010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8543"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VK031_01535"
     CDS             complement(<1..280)
                     /locus_tag="VK031_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52630.1"
                     /translation="MKKSNNNFSKKYNLGWVLTISIWTFLLAIIFSIIAENSIDRLGI
                     ISSFLILLLIILIGIAFDIIGIAVTRAEEKPYHSMAAKKIKEGKIAIKI"
     gene            complement(960..>1223)
                     /locus_tag="VK031_01540"
     CDS             complement(960..>1223)
                     /locus_tag="VK031_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HLS52631.1"
                     /translation="ASPREVKILREYAQVLGLSYQLQDDLLDIEEDESINKFTYLSFY
                     DKDKAEEDIERLCKESFDLLDKLDGRDTQFLKELTELLVNRKK"
BASE COUNT          364 a          262 c          180 g          417 t
ORIGIN      
        1 ctattttgat ggcaattttc ccctctttaa tttttttagc agccatagag tgatagggct
       61 tttcttctgc cctggttaca gctataccta ttatatcaaa tgctatacca attaggataa
      121 taagtagtaa aataagaaaa gaagatataa tccccagcct gtctatcgag ttttctgcta
      181 taatgctgaa aataattgct aataggaagg tccatatact gattgttagc acccaaccta
      241 aattatattt cttagaaaag ttattattgg atttcttcat agaaattttc ctcccatata
      301 taaaaagctg atagctaatc gctaccagca cacaagtggt gataaattga gtaaatttta
      361 cggcttaagg tccagcagga tttccctaat aaaaactatc tgtcgccaac atagcggttt
      421 ccctttaatt ttattactat tttgtcacca aaaatagagc tggattgcca ctgagtccgc
      481 actttaatcc ccaattcatc tcatgtgaac agtctaggag ttttcctcaa cagtcattct
      541 attccttagc ctcttttgca aaatagattt cgacaacaaa acttaaatat cactgggccc
      601 tagtatattt aagttgaact tgtcatccgc catatttcac tcaaggcagg ctactctgca
      661 cccaattttc tgcaggttta tcacaatata cagggtgttt attgtatatt gtgtctccac
      721 gaaagtaggg tccacgcctg catgccattg cagatcgccc catctttctt actccctgaa
      781 gaagcccacg actgggcgtc gcaagccaaa acaaggaact tcatcgatat gccctttgac
      841 ggatttttag ccccgccttc cagaatagat gtactgacta gaatactgca ccacttaaag
      901 ctataataat tatagcatag ttagtttttt aatacaatat tacaatattt tttcctaagt
      961 tatttttttc tatttactaa taactctgtt aattccttca aaaattgagt atctcttcca
     1021 tctaatttgt ctaataaatc aaaggattcc ttacaaagtc tttctatatc ctcttctgcc
     1081 ttatccttat cataaaagct aagataggta aatttattta tgctttcatc ctcttcaata
     1141 tctaataggt cgtcctgcaa ttgataagat aggccaagta cctgggcata ctctcttaaa
     1201 attttcactt cccttgggct ggc
//
LOCUS       DATGSI010000104         4561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_150523, whole genome shotgun sequence.
ACCESSION   DATGSI010000104 DATGSI010000000
VERSION     DATGSI010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150523"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VK031_01545"
     CDS             complement(<1..850)
                     /locus_tag="VK031_01545"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HLS52632.1"
                     /translation="MKRDSVSLLDHFVNFVIDRRKLVLFLYIIAVIFSIFSMGWVEVE
                     EDIIHYLSENSNTRQGLEIMDQELKSPGMADVMISNISYSRVEKIIEEVEKLEGVSTV
                     VFENTEDHYRNSSALFIVQFQEEETEQLTLDAMEGIRKIVEPYDHAISTTVGGNLADE
                     LTKDMTVVGILATIVVIVVLIFTSNSYGEIPVLLITFGVAALLNMGTNFLFGKISFVS
                     NSVAVVLQLALAIDYAIILCHRFNEERFVYDPVNAAKEALKKAIVEISSSSLTTIAGL
                     AALSFME"
     gene            complement(1100..1645)
                     /locus_tag="VK031_01550"
     CDS             complement(1100..1645)
                     /locus_tag="VK031_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52633.1"
                     /translation="MEKGYKYIFWGIFAATFSISLGPIRILPAFIAWAIVGAGLNEMK
                     EDTGEVFFDSAIKHNRFLTIVLFLLGLFSLMGLGIEGYFLFAYLSFIPAILELITVFK
                     ILEGTINFFNSRDEKDLCRDLIEDQRKYLIWQLLVISGGIILVNIPNHTFIFLAGILS
                     LGARIYLLALVSRLKNTILLD"
     gene            complement(1645..2529)
                     /locus_tag="VK031_01555"
     CDS             complement(1645..2529)
                     /locus_tag="VK031_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52634.1"
                     /translation="MELNKRLFKGGISVIIIVLLVSIGISYYLRLDEPVFLKVYMEKY
                     MPQDRKYHEDVSLEIKYITNIEDGRTVSFVEFQDYPELNVYSSEYGLNDLNFFYEEPG
                     LGQDIGRYNVRTIYIYLDTSNLKDYNEEIHINKARMHFSDGETKDVDIGKLILYSDKG
                     LSNYEHFQHISSSGRGDGSSSTREKIKEDIQLVKVEGVLLDQVKDFTEISIGNKDYRQ
                     IEGERYKSGSYLQIDTIFNKPDDAELKMYDYELKPKLIYRDGKGKEFLYRILNLHYYK
                     REFTYKEILEFLKVGGKI"
     gene            2749..4182
                     /locus_tag="VK031_01560"
     CDS             2749..4182
                     /locus_tag="VK031_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HLS52635.1"
                     /translation="MKFLMKNLRLILLLYVIYVLISGLFIFIRPKRISKGYIEKSSTD
                     RFFSNEIGPDRGILIDKPLDSGLARLKIIDSAKKSLNISYFSIEAGKSPSLFFAALID
                     AADRGVQVNILLDGIFHGLRGRNRSFIYTIINHPNMHLKLYEPINLLKPWTLNNRMHD
                     KYIIADGVIAIIGGRNIGDKYFAPEWYNKNVTNDRDIIIFNSDPKDTTSVLNQMTDYF
                     NQIWHHKYSKPIDKSLSRIQKKKALKTSDELRQQAKLIKKEYKNLFDEPIDLMEISFP
                     TRKVSFIHNPISRFSKEPWCWYEITELMKTAKNTIFAQSPYIIPNRQMIQGFLDKDEF
                     QDIEINILTNSLASTPNLPAYAGYMNYRKELVDSGLNIYEYQSKDSLHTKSFIIDDDL
                     LAIGSFNSDPRSTYLSTESMVIIHSKEAVEKIEEGLEEYLATSLLVDKDYGYVEKEGV
                     EILPVKPIKRISIKILSLIVRWFEYLL"
     gene            4262..>4561
                     /locus_tag="VK031_01565"
     CDS             4262..>4561
                     /locus_tag="VK031_01565"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLS52636.1"
                     /translation="MEVVYKIINDNEEQLELEASLYKNNSIDSKGIILYFHGGGLVMG
                     SRKDLPRYHIERLTSSGYPILSFDYRLAPEANFDQILSDVLDGIEYFINNKEKLGF"
BASE COUNT         1508 a          850 c          681 g         1522 t
ORIGIN      
        1 actccataaa ggataaggcc gccagaccag ctatagtggt taaactactg gatgatattt
       61 ccacaattgc tttctttaag gcttcctttg ctgcatttac cggatcataa acaaaacgtt
      121 cctcattaaa cctatgacaa aggattatag catagtctat ggccagggcc aattgtaaaa
      181 ctacagccac agaattggat acaaaagata ttttaccaaa taaaaaatta gtacccatat
      241 ttaataaggc agccactcca aaggtaatca aaagcactgg aatttctcca taggaattag
      301 atgtaaatat tagtacaaca atcaccacaa tcgtggctaa aatccctact acagtcatat
      361 ctttagtcaa ttcatcagcc aaatttcccc ctactgtagt ggatattgca tggtcatagg
      421 gttctactat tttccttatt ccctccatgg catctagggt taattgttct gtctcttcct
      481 cttgaaactg cactataaaa agggcagatg aattcctata atggtcctca gtattttcaa
      541 atactacagt agatacacct tccagttttt ccacttcttc tataatcttt tcaaccctgg
      601 aataactaat atttgatatc atcacatctg ccataccagg gctttttaac tcctggtcca
      661 taatctctaa tccttgccga gtattgctat tttcagatag gtagtggata atatcttctt
      721 ccacctctac ccagcccatg gagaatatgc taaatataac tgctattata tagagaaata
      781 atactaactt tcttctatct attacaaaat tcacaaaatg atctaataag cttacactat
      841 ctcttttcat cttctatcct tccttatagg ccaaggtatt gcttatggac tataattagt
      901 ccttttaaaa ttcttgcctt gaccggtagt taatatgcta aataacttaa ggttacaaaa
      961 tcatatctac attataacat agtttaatat gagagataaa ttctttaact agttgaaaat
     1021 tatacctttt aagatattaa aaagatgctt agtctgccaa actccaacca tgcatctttt
     1081 cggcctacag gattgaattt taatccaaca aaatagtatt ctttagccta cttaccaagg
     1141 ctagcagata aatcctagcc cctaaggaca aaattcctgc tagaaagata aaagtgtgat
     1201 tgggaatgtt taccaatata atacctcctg atattaccag caactgccag atcaggtact
     1261 ttctttgatc ctcaattagg tctcggcata gatccttttc atctcgggaa ttgaaaaaat
     1321 tgatcgtccc ttctaaaatc ttgaataccg taataagctc tagaatagca ggtataaagc
     1381 ttagataggc aaatagaaaa tagccttcaa ttcctagtcc cattaagcta aataggccta
     1441 gtagaaatag aactattgtt aaaaatctat tatgctttat ggcagaatca aaaaatactt
     1501 ccccagtatc ctctttcatt tcatttaggc ctgccccaac tatggcccaa gctataaatg
     1561 caggtaatat tctaatgggt cctaagctta tactaaaggt ggcagcaaaa ataccccaaa
     1621 atatgtattt gtatcccttc tccattagat cttcccccca acttttaaga attctaatat
     1681 ttccttatat gtaaactccc tcttataata gtgcaaattt aagatcctat ataaaaactc
     1741 tttacctttt ccatccctat agattagttt aggttttaat tcataatcat acatctttaa
     1801 ttctgcatca tctggtttgt taaaaattgt atcaatttgt aggtaagaac ctgacttata
     1861 cctttctcct tctatttgcc tatagtcctt attccctata cttatttccg taaaatcctt
     1921 tacttggtcc aagagtaccc cctcaacctt aactagctgg atatcctcct taatcttttc
     1981 tctggtgcta cttgacccat ctccccttcc actgctggaa atatgttgaa aatgttcata
     2041 gttagatagc cccttgtctg aataaagaat taattttcct atatccacat cttttgtctc
     2101 tccatctgaa aagtgcatcc tggccttatt aatatgtatc tcctcattat aatccttcaa
     2161 attgctagta tctaagtata tatatatggt ccttacattg tacctaccta tatcctgccc
     2221 taagcctggc tcttcataaa aaaagtttaa atcatttaga ccgtattcgg aagaatatac
     2281 attaagctct gggtagtctt gaaattccac aaaactaaca gtccttccat cctctatatt
     2341 agtaatgtat tttatttcca aacttacatc ttcatggtat tttctatcct gtggcatata
     2401 cttttccata tacactttta aaaacactgg ctcatctaat cttagataat aggatattcc
     2461 tatagatact aacaaaacta taataattac acttatacca cctttaaata atcttttatt
     2521 taactccatt atctgtctcc tatcctttaa tattttgtat tgcttccttc ctcctaatta
     2581 tctttgtatt atttatattt aatataagtt catactaaca aataatacag attaggtcta
     2641 ctatttacct gtttatagct tataataatt aattgacaag gctttattat tgtttaataa
     2701 tatctatagg ttgattgaat caatagatta cttaagaaga gggtatatat gaaattttta
     2761 atgaagaatc tcagattaat tctactgtta tatgtaatat atgttcttat cagtggcctg
     2821 tttatattta tacggcctaa aagaatttca aaaggatata ttgaaaaatc ttccactgac
     2881 aggttttttt ctaatgaaat tgggccagat aggggtatat tgatagacaa gcctttggac
     2941 tctggactgg ccaggcttaa aatcattgac agtgccaaga agagtctaaa tatatcctac
     3001 ttttccatag aggctgggaa aagccctagc ctattctttg ctgccctaat agatgcggct
     3061 gataggggag ttcaggttaa tatcttgtta gatggaattt ttcacggact aagagggaga
     3121 aatagatcct ttatttatac aataattaac catcctaata tgcatcttaa actatatgag
     3181 cctattaacc tgctaaaacc ttggacttta aataatagga tgcatgataa gtatattatt
     3241 gcagatgggg taattgccat tataggtggt agaaatattg gggacaagta tttcgctcca
     3301 gaatggtaca ataaaaatgt aacaaatgac agggacatta taatatttaa ttcagaccca
     3361 aaggatacca ccagtgtcct aaatcaaatg acagattact ttaaccaaat ctggcaccat
     3421 aagtattcta aacctattga taagtcctta tctagaattc aaaagaaaaa ggccctgaaa
     3481 accagtgatg aacttagaca acaagcaaag cttattaaga aagaatataa aaatctcttt
     3541 gatgaaccta tagacttaat ggagatatct tttccaacca ggaaggtttc ctttatccat
     3601 aatccaatta gccgcttttc aaaggagccc tggtgctggt atgaaatcac tgaacttatg
     3661 aaaacagcta agaataccat atttgctcaa agtccttata ttatacctaa caggcaaatg
     3721 atccaaggtt ttctagataa agatgagttt caggacatag agataaatat cctgactaat
     3781 tccctggcca gtacccctaa cctacctgcc tatgctggat atatgaatta tagaaaagaa
     3841 ttggtagata gtggcttaaa tatatatgaa taccaatcaa aggattcctt acacacaaaa
     3901 tcctttataa tagatgatga ccttttggcc attggttctt ttaattcaga tcctagaagc
     3961 acttatttaa gtacagaatc tatggtcatt attcatagca aggaggctgt agaaaaaata
     4021 gaggaaggct tagaagagta tttggccacc agtctactgg tagataagga ttatggctat
     4081 gtagaaaaag aaggtgtaga aattttacct gtaaagccaa ttaaaagaat aagtataaaa
     4141 atcttatccc ttatagtaag gtggtttgag tatttgctct aatttttcat actagatggg
     4201 gcatatatct tctttagggt atattataaa tactataaat tatgggatta tggaggtttc
     4261 aatggaagtt gtatataaaa ttataaatga caatgaagag caattagaac tggaggccag
     4321 cttgtataaa aacaattcta ttgatagcaa aggaattata ctttattttc atggtggtgg
     4381 gctggttatg ggtagtagga aggatctacc tagataccat atagaaagac tgaccagctc
     4441 tggctaccct atcttatcct ttgattatcg actagctcct gaggctaatt ttgaccaaat
     4501 tttatcagat gttttagacg gtatagaata ctttataaac aataaggaga aattaggttt
     4561 t
//
LOCUS       DATGSI010000105         1041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_8581, whole genome shotgun sequence.
ACCESSION   DATGSI010000105 DATGSI010000000
VERSION     DATGSI010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8581"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VK031_01570"
     CDS             <1..956
                     /locus_tag="VK031_01570"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HLS52637.1"
                     /translation="IKYEDAINILGVTNRDLIFDLVDRMKAEKTEDVLVAIDEIIQSG
                     KDVGQFIKDLIEHFRNLMVVKSSKSPELIIEVSNMDKYIEQSNLLDLDYILEALDILA
                     EADSQAKWSSQPRIILEMATLKLINLNKSLSLEERIKRLEQGLGPTYKERDIPEGLEI
                     KSGEDKMVEEEKKQPAFEDKEEKLVDKRIEPVKFKDDGGELDFDTINQEWPKVLQVIN
                     RKDVKTHAFLIEGEILSFRGNLLTIGYKEGFGFHQQAINTPKRKELVEKIVSEYFNRN
                     IRINFVISNNMPKREEKKETSKHKAVKEVVDFFGEDIVEIK"
BASE COUNT          406 a          124 c          247 g          264 t
ORIGIN      
        1 tgataaaata tgaagatgca attaatatat taggtgtcac caacagagac ttaatttttg
       61 acctggtaga caggatgaag gctgaaaaga ctgaggatgt attggttgct attgatgaga
      121 taatccaaag tggcaaggat gtgggacaat ttataaagga ccttatagag cactttagga
      181 atttaatggt agtaaagtca tctaaaagcc ctgaacttat aatagaagtc tctaatatgg
      241 ataaatatat agagcaaagc aacctacttg atttggatta tatattggag gccctagaca
      301 tactggcaga agcagattct caggctaaat ggtccagcca gccaagaatt attttggaaa
      361 tggcaacact gaaattgatt aacctaaata aaagcttatc cctggaggag agaattaaaa
      421 gactggagca gggtttaggt cctacttata aggagcgaga cataccggag ggtcttgaaa
      481 taaaatcagg ggaagataaa atggttgaag aggagaaaaa gcaaccagcc tttgaagaca
      541 aggaagaaaa gctagttgat aaaagaattg agcctgtaaa gtttaaggat gatggaggag
      601 aacttgattt tgatactatt aaccaggaat ggccaaaggt attgcaggtg attaacagga
      661 aagatgttaa gacccatgct tttctaatag agggtgagat cttgtccttt aggggaaatt
      721 tattaactat aggatacaag gaaggctttg gttttcacca gcaggcaata aatacgccaa
      781 agagaaaaga attagtggaa aaaatagtat cagaatactt taatagaaat ataaggatta
      841 actttgttat ttctaacaat atgcctaaaa gggaagaaaa gaaagaaact agtaagcata
      901 aggctgtcaa ggaagtcgtg gacttttttg gagaagatat tgtggaaatt aaatagaaag
      961 gaagatttaa attatggcaa gaggaagatt ccaaggaatg ggaggaaata tgggcaatat
     1021 gatgaaacaa atgcaaaaaa t
//
LOCUS       DATGSI010000106         1324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_55297, whole genome shotgun sequence.
ACCESSION   DATGSI010000106 DATGSI010000000
VERSION     DATGSI010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55297"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VK031_01575"
     CDS             <1..753
                     /locus_tag="VK031_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLS52638.1"
                     /translation="VKTDVKEIIELSEAIGFLGNTLAEQEDIRKRYASDISHELRTPL
                     TTLRTHLEAIIDGVWEPTREHLQILMEEIQRLSNLVDNLRDSFTETEYSIKLNKERFN
                     ISKELEAIITAYVPIYEKKGFTILGSIEPNIEVYMDKDKFKQIINNLLSNSLRYLEDE
                     GQVFVDLKENNRKITIKVKDNGIGIKDKDLPHIFNRFYRVDTSRQKSTGGSGLGLSIV
                     KSMVEAHGGYIYVESQYGKGTEFTLNFPLKQT"
     gene            complement(728..1186)
                     /locus_tag="VK031_01580"
     CDS             complement(728..1186)
                     /locus_tag="VK031_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLS52639.1"
                     /translation="MKKSILLIYLTWVGHKRQIEKPSDLLLFTLRNQLGLTGAKPGCE
                     NGDCGACTVLVDGWPIKSCVMLTVEAIGKKITTIEGLKNAPIQKAFVEEIPFQCGYCT
                     SGFLMVCHGLSEIHPDADESVIEEWLQSNICRCTSYQEIGKAIKSVLRGS"
BASE COUNT          430 a          239 c          255 g          400 t
ORIGIN      
        1 gtgaaaaccg atgttaaaga gattattgaa ctgtcagagg ccataggttt tcttggtaat
       61 accctggcag aacaggaaga cataagaaaa agatatgcct cagacatatc ccatgagtta
      121 aggacccctc ttacaacctt aagaacccat ctggaagcca taattgatgg agtctgggag
      181 cctaccaggg agcatttaca aattcttatg gaagagatcc aaagactatc aaacctggta
      241 gataatttgc gggattcttt tactgaaact gaatatagta ttaaactaaa taaagaaagg
      301 tttaatatat caaaggagct tgaggctatt attactgcct atgttcctat ttatgagaaa
      361 aaaggtttca caatcttggg ctccattgaa cctaacatag aagtctatat ggataaggat
      421 aagtttaagc aaattataaa taatcttcta tccaattccc taagatattt ggaggatgag
      481 ggtcaagttt ttgttgattt aaaggaaaat aacagaaaga tcaccatcaa ggtgaaagat
      541 aatggaatag gtataaaaga caaagatttg cctcatatat ttaataggtt ttatagggtt
      601 gacacatcta ggcagaaatc tactggagga tcaggcctgg gcctatctat tgttaagtcc
      661 atggttgagg cccatggtgg atatatatat gtggaaagtc aatatggtaa ggggactgaa
      721 tttaccctta acttcccctt aaaacagact taatggcctt gcctatctct tgataactgg
      781 tacacctaca aatatttgac tgtagccatt cttctattac actttcatct gcatctggat
      841 ggatttcaga taaaccgtgg cagaccatta aaaaaccaga ggtacagtat ccacactgaa
      901 agggaatttc ctctacaaag gctttctgga ttggtgcatt cttgagtcct tctatggttg
      961 ttattttctt tcctatagct tctaccgtaa gcattacaca ggactttata ggccaaccgt
     1021 ctaccaaaac tgtacaggct ccacaatctc cattttcaca acctggcttg gctcctgtca
     1081 ggcctaactg gttacgcaag gtgaataata aaagatctga aggcttttca atctgccttt
     1141 tatggcctac ccaggttaaa tagataagca atattgattt cttcaataaa taaaattacg
     1201 gagccattgg ttattggtat aataacatct tttaattgct ttacctcaat ggtccctgat
     1261 ttatatatgg tggaccttaa atctcaggga gataataaat cctgcatcaa aggtttaagt
     1321 aggt
//
LOCUS       DATGSI010000107         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_480671, whole genome shotgun sequence.
ACCESSION   DATGSI010000107 DATGSI010000000
VERSION     DATGSI010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480671"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VK031_01585"
     CDS             <1..890
                     /locus_tag="VK031_01585"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HLS52640.1"
                     /translation="AIISAIMGHVYVISGGVVEEFHLMQTILILFIGIIVNSVVISTL
                     ISITEGKKFTEVCFSSLKGIMLNAGSVGILGIIMALAHLSYGFGAVVLFFGPLLLARH
                     SFKLYIDMRNVYISTIETLGKIIEAKDPYTSGHANRVKKYSIMLAEAYGLDYDRIQNI
                     KRAAVLHDIGKIAINDNILHKSTKLTQSEFAHIMKHPTVGADIISEMDFLKDVADIIR
                     YHHERYDGTGYPEGIGGDEVPLEAYILAIADAYDAMTTDRPYRKALERKEALKEIGKN
                     AGSQFHPSLAEKFISIMAN"
     gene            906..1478
                     /locus_tag="VK031_01590"
     CDS             906..1478
                     /locus_tag="VK031_01590"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HLS52641.1"
                     /translation="MLEAIIISILVAKLRKGRLENLEKVGFKGRYLLLMAAATQLTMS
                     LFKKIGFNIEGSGIINWFFLFHFMSYVLILVAITLNIEKRSMKLFLIGVILNFLVIFA
                     NGGKMPVSIEGIKGINKRDSIELLISDFDIKHKSIDENTRLIYLADIILIPKPYPLPK
                     VLSVGDLFLMGGFFLFMQEGMINKDEKCKA"
     gene            1571..2821
                     /locus_tag="VK031_01595"
     CDS             1571..2821
                     /locus_tag="VK031_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HLS52642.1"
                     /translation="MGLNNKGTAILKEDNINIQPVLLGGDINCYSVARAFHEEYGVKS
                     IAFGRYALGATKDSKIIDFRIDKQMEDRDKLMENLLKLGDKLEDAGKTPILMGCTDEY
                     AEFIIDNKEILSDKYVTPYIDKELKDKLIEKELFYQMCEKKGLDYPKTYIYSKGDKVT
                     EFDFDYPVIIKPSDSVLYWQHPFEGMKKVYIAKNKEEFSQITDTIYKGKYNKSLIIQD
                     FIPGDDSHMRVLTCYSDRHGKVKMMCLGHVLLEEHTPTAIGNHAAIITEYNQELMEKI
                     KDFLEDINYTGFSNFDIKFDPRDNKYKLFEINLRQGRSNYYVTSSGNNIAKYVVEDRV
                     LNKDLDLSIQKDPFYWHVVPNSVVYKYVKDKVLVREAKKLVAEGKEATSFGYREDLRG
                     NFRRRLYVFLYNINQIRKYKKYFE"
BASE COUNT         1090 a          356 c          609 g          907 t
ORIGIN      
        1 gtgctattat ttctgctatt atgggacatg tatatgtaat aagtggaggt gtagttgagg
       61 aatttcattt aatgcaaact atattgatat tatttattgg tataatagta aattccgttg
      121 taatctccac cttaatttcc ataactgaag ggaagaaatt tactgaagtc tgtttttcaa
      181 gtttaaaggg tattatgttg aacgctggat cagtaggtat tttaggaatt ataatggcct
      241 tggcccactt aagttatgga tttggagcag tagttttatt ctttgggcca ttgctgttgg
      301 ctaggcattc atttaagcta tatatagata tgaggaatgt ttatatatca actatagaaa
      361 cattaggaaa aattatagaa gctaaggacc cttatactag tgggcatgcc aatagggtaa
      421 aaaaatattc aattatgttg gctgaggctt atgggctaga ttatgacaga atccaaaata
      481 ttaaaagggc agcagttctt cacgatattg gtaagattgc cattaatgac aatattttac
      541 ataaatccac taagctgact caaagtgagt ttgcacatat tatgaaacat ccaactgtgg
      601 gagcagatat aattagtgaa atggattttt taaaagatgt agcagacatt atcaggtatc
      661 atcatgaaag atatgatggt acaggctatc cggaaggtat aggtggagat gaagtccctt
      721 tagaagccta tatattggct attgcagatg cctatgatgc catgacaaca gataggcctt
      781 atagaaaggc cttggaaaga aaagaggctt taaaggaaat tggaaaaaat gcaggcagtc
      841 aatttcatcc aagcttggct gaaaagttta tatccataat ggctaattag ggagctgatt
      901 atttagtgtt agaagctatt attatttcta tactggtggc taaattaaga aaaggtaggt
      961 tggaaaattt agagaaggta ggatttaaag gccggtatct cttgcttatg gctgcagcaa
     1021 ctcagcttac tatgtctctg tttaaaaaga taggttttaa tatagaaggt agtggaatta
     1081 ttaattggtt ttttcttttt catttcatgt cctatgtatt aatacttgta gcaataactt
     1141 taaatattga gaaaagatct atgaaattat ttttaatagg tgtaatcctt aattttttgg
     1201 ttatctttgc taatggcggc aagatgcccg tctcaataga aggaataaag ggaataaata
     1261 aaagagatag catagaactg ttgattagtg atttcgatat aaaacataag tctattgatg
     1321 aaaatactag acttatttat ctagcagata ttattttaat accaaagcct taccccttac
     1381 ctaaagtact aagtgtaggg gacttatttt taatgggagg attctttcta tttatgcaag
     1441 agggtatgat aaataaagat gaaaaatgta aggcttagtc tggtatagtt tagcaattaa
     1501 aggaaatgga aaacatataa aatatataaa atatgtaagc aacacaataa gataaatggg
     1561 aggttgtcaa atgggattaa ataataaagg tacagcaatt ctaaaagaag ataatataaa
     1621 tatacaacca gttttactag gtggagatat taactgttat agtgttgcaa gagcctttca
     1681 cgaagaatat ggagtaaagt ccatagcctt tggaaggtat gccctaggtg caacaaaaga
     1741 tagcaaaata atagattttc gaattgataa gcagatggaa gatagggaca aactaatgga
     1801 aaacctgcta aaacttggag ataaactgga agatgcaggt aagactccaa tcctaatggg
     1861 ttgtacagac gaatatgcgg agtttatcat agataataaa gaaatattat ctgacaaata
     1921 tgttactcct tacattgata aggaattaaa ggataaatta atagagaagg aacttttcta
     1981 tcaaatgtgt gaaaaaaagg ggctagatta tcctaaaaca tatatatatt caaagggaga
     2041 taaagtaaca gaatttgact ttgactaccc agttataata aagccctcag atagtgtttt
     2101 atattggcaa catccttttg agggaatgaa aaaagtatat atagctaaaa acaaggaaga
     2161 atttagtcaa attacagata ccatatacaa gggcaagtat aacaaaagcc ttataattca
     2221 ggattttatt cctggcgatg attctcatat gagggtactg acttgttatt cagacaggca
     2281 tggaaaggtt aaaatgatgt gtttaggcca tgttttattg gaggagcata cacctactgc
     2341 cataggaaac catgcagcca taataacaga atataaccag gaacttatgg agaaaataaa
     2401 agactttttg gaggatataa actatactgg cttctcaaac tttgatatta aatttgatcc
     2461 aagggataat aaatacaaac tatttgaaat taacctaagg caaggtagaa gcaattatta
     2521 tgtaacatca tcaggaaata atatagctaa gtatgtggtg gaagataggg tacttaacaa
     2581 ggatttagat ttaagcattc aaaaggatcc cttctattgg catgtggtac caaacagtgt
     2641 agtgtataaa tatgtaaaag ataaggtttt ggtaagggag gcaaaaaaac ttgtggctga
     2701 aggtaaggag gctacttcct ttggttacag ggaagattta agaggaaact ttagaaggcg
     2761 actttatgta tttctttaca atattaatca aataagaaag tataaaaaat actttgagta
     2821 gaggtacttt ttaagcctta gctagaaaaa agtatattaa aggagagtca ggatgaagtt
     2881 aaaggatgtt tttcaaggag tttctacaag gtctaacaag gactgggaag aaatacaaga
     2941 tataaagata aagggtattt ca
//
LOCUS       DATGSI010000108         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_151036, whole genome shotgun sequence.
ACCESSION   DATGSI010000108 DATGSI010000000
VERSION     DATGSI010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151036"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VK031_01600"
     CDS             complement(<1..622)
                     /locus_tag="VK031_01600"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HLS52643.1"
                     /translation="MESILMPFCISAFITIILVPLVINLAKEIGALDIPKDNRRMHKI
                     PMPLMGGLAIYIGVVISMLIFLPITIPLLSVIVAGSLVVISGIIDDIKDISPKFKLLF
                     QLLASIVLIIGGVRITFITNPFSNSQQLFNLGWLSAPISLIWIIGITNTLNLIDGLDG
                     LAAGIALISSLSLLVVAYSLGYTNTMILSAILAGACLGFLPFNFRPA"
     gene            complement(771..1400)
                     /gene="upp"
                     /locus_tag="VK031_01605"
     CDS             complement(771..1400)
                     /gene="upp"
                     /locus_tag="VK031_01605"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014471148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HLS52644.1"
                     /translation="MGKVIVLDHPLIKHKLTFIRDKNTGSKEFRELVSEVSMLMGYEV
                     TRDMELEEIEIETPMTTMKSEVIAGKKVGIVPILRAGLGMVDGILSLIPTAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIDERTLIVLDPMLATGGSAVAAIDFLKGKGANQIKLVCL
                     IGAPEGVELVRKSHPDVDIYLANIDEKLDENAYIIPGLGDAGDRLFGTK"
     gene            complement(1447..1884)
                     /gene="rpiB"
                     /locus_tag="VK031_01610"
     CDS             complement(1447..1884)
                     /gene="rpiB"
                     /locus_tag="VK031_01610"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HLS52645.1"
                     /translation="MKIGIGSDHGGFELKKAIREFLEQEGIDYIDYGTDSTDSVDYPD
                     FGEKVARAVVDKEIDRGIVVCGTGIGISIAANKVKGIRCALCGDVFSARMSRAHNNAN
                     MLALGGRVLGVGLALEIVKAWLETEFEGDRHERRLIKIDEIKS"
     gene            complement(2012..>2209)
                     /locus_tag="VK031_01615"
     CDS             complement(2012..>2209)
                     /locus_tag="VK031_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52646.1"
                     /translation="LPFKILKYHDTVENRFSNSLRSNSENLTIFFLSRILDRIFHKSG
                     LPLQIYPQIQQKDWERQDFNI"
BASE COUNT          668 a          473 c          322 g          746 t
ORIGIN      
        1 tggccggtct aaaattaaaa ggtaaaaaac ctaaacaggc accggctaat atggctgata
       61 aaatcattgt atttgtatag cctaaagaat aggcaaccac taggagtgat aagctggata
      121 taagggcaat acctgctgcc aacccatcca gtccatctat aaggtttaga gtatttgtaa
      181 ttcctataat ccaaattaaa ctaatagggg cagatagcca gcctaaattg aaaagttgtt
      241 ggctatttga gaagggattg gtaataaatg ttattcttac accacctatt attaatacaa
      301 tgctagccag gagctgaaag agtagtttaa attttgggct tatatcttta atatcatcta
      361 taattcctga tatgaccacc agacttccag ccactatcac tgataaaagg ggaattgtta
      421 taggtaaaaa tattagcata gatataacca ctccaatata gatggctaat cctcccatta
      481 aaggcatagg gattttatgc atccttctat tgtcctttgg tatatctagg gcaccaattt
      541 ctttagccaa atttattacc aatggaacca gaataatggt tataaaggca gatatacaaa
      601 aaggcattag aatactttcc attctgtctc tcctcttctt taaatagtat tagtatatgt
      661 atttatcctt tttataacca aataaaaaac agcctgccac ctatttagta taggcaacaa
      721 gccgtttaat caatatttgc aagtcaagat taaagtgttg tcaaccattc ctacttggtt
      781 ccaaacaatc tatctcctgc atctcctaaa cctggaatta tatatgcatt ttcatctaat
      841 ttttcatcaa tattagccag atatatgtcc acatctggat gagactttcg aaccaactct
      901 actccctctg gtgcaccaat taaacaaact aatttaattt gattggcccc cttacctttt
      961 aaaaagtcta tggcagccac tgctgaacca ccggttgcca acatagggtc tagaactatt
     1021 aaggtcctct catctatatc ttgtggtagt ttacaataat attccactgg ctttaaggtt
     1081 tcagggtccc tataaagccc cacatgtcct accttggcag ttggtattag gcttaaaatc
     1141 ccatccacca ttcctaaacc tgccctaagt atgggaacta tgcctacctt ttttccggct
     1201 attacctctg atttcatagt agtcatggga gtttctattt ctatctcctc tagttccata
     1261 tcccttgtga cttcatatcc cattaacatt gatacttctg aaactaattc tctaaattct
     1321 tttgaacctg tatttttatc cctaataaat gttaatttat gcttaattag aggatgatct
     1381 aatactatga ctttgcccat tataattcct cccttttaat ttaatcctat aggattatat
     1441 catactttaa gactttattt catcaatttt tataagtctt ctttcgtgcc tgtcgccttc
     1501 aaattctgtt tcaagccaag cttttactat ttccagggca agtcctaccc caagtaccct
     1561 gccacctaaa gccaacatat tagcattatt atgtgcccta ctcattctag ctgaaaacac
     1621 atctccacat agggcacatc ttatcccttt taccttattg gctgctatag agataccaat
     1681 tcctgttcca caaactacta tacccctatc tatttcttta tccactacag cccttgccac
     1741 cttctcccca aaatcagggt aatctacaga atctgtagaa tcagtaccat agtcaatata
     1801 gtcaatacct tcttgttcta aaaattccct aatggctttt tttaattcaa agccaccatg
     1861 gtcacttcca atacctattt tcatttgtaa atccaccttt ctttttatct aagtttcata
     1921 taatatggaa aattcgaagc agagaataaa tttttcaaag tccagtctta attaaaaaat
     1981 ttattcttcc ttccctggcc ttgtgtagaa tttagatatt gaaatcctgc ctctcccagt
     2041 ccttttgttg gatttgggga tatatttgca agggtaagcc tgacttatga aagattctgt
     2101 caagtatccg actaaggaag aaaatcgtta ggttttcact gtttgagcga agcgagtttg
     2161 aaaacctatt ttcgaccgtg tcgtgatact taagaatctt gaatggaag
//
LOCUS       DATGSI010000109         1575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_622248, whole genome shotgun sequence.
ACCESSION   DATGSI010000109 DATGSI010000000
VERSION     DATGSI010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622248"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VK031_01620"
     CDS             <1..590
                     /locus_tag="VK031_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52647.1"
                     /translation="NSFYGYQVDGIIQEMPDNPVSLTSAGELNYIGLNSDGTLNSNAR
                     TIIGDPNPDFTSSFNLQLRHQSGFDLSILLYLVYGRDIFSTRRLNRPSLEEKRWTAEN
                     PNNKYPSLRSTRQYFASSWFIEDGSFFRIKNITLGYTLPEISFLQKGRAYINVSNPFT
                     FSKTTEYDPEVGENGRGGTPYPRVMMLTAGLEFQF"
     gene            676..>1575
                     /locus_tag="VK031_01625"
     CDS             676..>1575
                     /locus_tag="VK031_01625"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLS52648.1"
                     /translation="MKENPHGSYTTDNYFSTESDALNALLYAYAPINYIEYGARFRFN
                     IADVTTNQYTSYNKGLETGFYTWDINPNTEEFLYFFKYVYLSITRANAVLENVSTMNN
                     IDPIVKEQFLGEAYFLRAFNYFMLVRTFGEVPLHEEVIKNMDMGQRSYASIETLYEFI
                     ISDLEEAISKMDIDKQPGRADKVAAQSLMSKVYLTMASSKMTGAPGYEWVSNYDEMYA
                     KAAKYAGEVLYDQSTYGLEPDLLKIYDVEHQNDSIEHIFITSMSREGKGQEGSFSQLP
                     QMYGIGGLQEIYISSSLDNKDDDV"
BASE COUNT          530 a          243 c          293 g          509 t
ORIGIN      
        1 ttaactcctt ctatggatat caggtagatg gaattataca ggaaatgcct gataatccag
       61 tttcactaac ttcagccgga gaattgaatt atattggatt aaattcagat gggacactca
      121 actctaatgc acgaaccatc ataggtgatc ctaatccaga tttcacatca agttttaatt
      181 tacaattgcg acatcaaagt ggatttgatt tatctatatt attataccta gtatatggca
      241 gagatatttt ttcaactaga agactaaacc gaccatcttt agaagagaaa cgttggactg
      301 ctgaaaaccc aaataataaa tatcctagcc tccgttccac aaggcaatac tttgcctctt
      361 cttggtttat agaagatggc tctttttttc ggataaaaaa tattactctt gggtatactc
      421 ttccagaaat cagttttttg caaaaaggtc gggcctatat aaatgtgagt aatccattta
      481 cattttcaaa gacaactgaa tatgatcctg aggtaggtga aaacgggcga ggaggaacac
      541 cttatcctag agttatgatg ttgactgcag gtttggaatt tcaattttaa tatctcaaat
      601 aagataaaag atatgaaaaa tcttttgata ataatgggtt tttttcttct cactttcaca
      661 tcgtgtgata atctgttgaa agaaaatcct catggatcat atactaccga taattatttt
      721 agcactgaat ctgatgcatt gaatgccctt ttatatgctt atgcccctat taattatata
      781 gaatatgggg ctcgatttcg tttcaatata gcagacgtaa ctaccaatca atacacatct
      841 tataataaag gtctagaaac tggtttttat acttgggata taaaccctaa tactgaggag
      901 ttcctttatt tttttaaata cgtttattta agtattacca gagctaatgc tgtattagag
      961 aatgtaagta caatgaataa tattgatcct attgtaaaag aacaattttt aggagaagct
     1021 tattttttac gtgcatttaa ttacttcatg ctggttagga cctttggaga agtaccacta
     1081 catgaggaag tgataaaaaa tatggatatg ggacaaagat cctatgcaag tatcgaaacc
     1141 ttatatgaat ttatcataag tgatttagaa gaggctattt ctaagatgga tatagataaa
     1201 caacctggaa gggccgataa agtagccgca cagtcactaa tgtcaaaggt ataccttact
     1261 atggcatctt ctaaaatgac cggggctcct ggttatgagt gggtatcaaa ctatgatgaa
     1321 atgtatgcaa aggctgcaaa atatgctggg gaggttttat atgatcaaag tacgtatggg
     1381 ctagagccag accttttaaa gatatatgat gtcgaacatc aaaatgatag tattgaacat
     1441 attttcatta cttcaatgtc gagagaagga aaaggacaag aagggagttt ttcgcaactt
     1501 ccacagatgt atggtattgg tggtctacaa gagatttata tttcatcttc tctggataat
     1561 aaagatgatg atgta
//
LOCUS       DATGSI010000110         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_151227, whole genome shotgun sequence.
ACCESSION   DATGSI010000110 DATGSI010000000
VERSION     DATGSI010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151227"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="VK031_01630"
     CDS             complement(<1..124)
                     /locus_tag="VK031_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004834820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HLS52649.1"
                     /translation="MVFESLSEKIQNALGKLTGKGKLSEKDVDAAMREVRLALLE"
     gene            complement(137..505)
                     /locus_tag="VK031_01635"
     CDS             complement(137..505)
                     /locus_tag="VK031_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HLS52650.1"
                     /translation="MVEKLVEVGILFDFYGKLLSKRQYLVVDLFYIHDLSLAEIGEEL
                     GITRQGVYDALKRAEIKLYQYEDRLGLVNKFRKNHNQMEEILKITEEIKALAKKEANS
                     LVIDKINLIENKVAKILKNS"
     gene            complement(588..1589)
                     /gene="ftsY"
                     /locus_tag="VK031_01640"
     CDS             complement(588..1589)
                     /gene="ftsY"
                     /locus_tag="VK031_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HLS52651.1"
                     /translation="MFKWFKKEDEKEKESTTRKDLIEETGEKKQSFLDKLVTGLTKTR
                     KEMSNKIDSILSFYTRIDEELFEDLEEVLVTADVGVNTTMKLIDNLREKVKSEKVTEP
                     AQVKEMLKDEIKNLMNSSIEDNTLNVEPAPAVILVVGVNGVGKTTTIGKMAHNFKKAG
                     KKVLIAAGDTFRAAAIEQLEEWGNRAGVDIVAHSEGADPAAVIYDSIQAAKARKTDIL
                     ICDTAGRLHNKSNLMNELNKIFRVIGNEFPEASLEVLLVLDATTGQNAISQAKEFKEV
                     ADITGVALTKLDGTAKGGVVVALQSELALPIKLIGIGEGIDDLQPFNGDAFVDAIFD"
     gene            complement(1600..>2192)
                     /locus_tag="VK031_01645"
     CDS             complement(1600..>2192)
                     /locus_tag="VK031_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HLS52652.1"
                     /translation="LDEYDKVKERIDFLVEQKEDLTSSKERLEEIIIDMEKKMRTIFV
                     KQFKEINENFREIFSILFGGGKAMLRLDEEDILGAGIEIVAQPPGKRLQNINLLSGGE
                     KTLTAVALLFAILKTRPSPFCILDEIDAALDEANINRYTDYLKSFNQDVQFVLITHRK
                     TTMEIADILYGVTMAEEGVSKLISVELKDYIDDMAG"
BASE COUNT          578 a          504 c          286 g          824 t
ORIGIN      
        1 cctccagtag tgccaacctt acttctctca tggctgcatc tacatctttt tctgaaagct
       61 tcccctttcc tgttaatttt cctaaagcgt tttgtatttt ttcggataga ctttcaaaca
      121 ccatagtttt agccccctag ctattcttta atatttttgc caccttattt tcaattagat
      181 ttattttatc tattactaaa gaatttgcct ccttcttcgc aagggccttt atttcttcag
      241 taatctttaa tatttcttcc atttgattat ggttctttcg aaatttgtta accaggccta
      301 acctgtcctc atactggtat agtttgatct ccgccctttt aagagcatcg taaactccct
      361 gtcttgtaat tcctagctct tcacctattt ctgctagaga taagtcatgg atgtaaaata
      421 agtctaccac caagtattgt cttttactta gaagtttacc ataaaaatca aacaatatac
      481 cgacttccac caatttctca accattatat caccattaaa atagtactgt caaggattat
      541 ccttgacagt actattttaa tttattaatt accttttgtc aatcccttta atcaaatatt
      601 gcatctacaa aagcatctcc attaaagggc tgtaaatcat caattccttc cccaatccct
      661 atcaacttta tgggaagggc caattcagat tgtaaggcta ctaccactcc accctttgca
      721 gttccatcta acttagttag ggctactcct gtaatatctg ccacttcttt aaattcctta
      781 gcctgactaa ttgcattttg tcctgtagta gcatctaaaa ctaataatac ctctaaagaa
      841 gcttcaggaa attcatttcc tataaccctg aaaattttat ttaattcatt cattagattg
      901 gatttattgt gcaagcgtcc tgctgtatca cagattaaaa tatcagtttt tctggcctta
      961 gcggcttgga tactatcgta aataactgcg gcagggtctg ccccttcgct gtgagcaact
     1021 atatctactc ctgccctgtt tccccattct tccagttgtt ctatagctgc agcacggaag
     1081 gtatcccctg cggctattag gacctttttg cctgcctttt taaaattgtg ggccattttc
     1141 cctatggtgg ttgtctttcc taccccgttt acacctacca ctaggattac tgcaggagct
     1201 ggctccacat ttagagtatt gtcttcaata gagctattca tcagattttt gatctcatct
     1261 tttaacattt cttttacctg ggccggttct gtaacttttt ctgatttaac cttctccctt
     1321 agattatcta ttaatttcat agttgtattg actcccacat cagcagttac caatacctct
     1381 tccaaatctt caaatagctc ctcatctatt ctagtgtaga aggataggat actgtctatt
     1441 ttattagaca tttcttttct agttttagtt agaccagtaa caagcttatc taaaaaactt
     1501 tgctttttct cccctgtttc ttctattaaa tctttacggg tagttgactc tttttccttt
     1561 tcatcttctt ttttaaacca tttaaacata attaacctcc taacctgcca tatcatcaat
     1621 atagtctttt aattctacag aaattagttt actaacacct tcttctgcca tagtaacacc
     1681 gtataatata tcagcaatct ccattgtagt cttcctatgg gtaatcaaaa caaattgtac
     1741 atcctggttg aaacttttta aatagtctgt atacctgtta atattggctt catctaaggc
     1801 tgcatctatc tcatctaaaa tgcagaaagg agagggcctg gtctttaaaa tagcaaaaag
     1861 taaggctact gcagttaagg ttttttctcc acctgatagt aggtttatgt tttgcaacct
     1921 ttttcccgga ggttgagcca ctatttcaat ccctgctcct aatatatctt cctcatctag
     1981 ccttaacatg gccttgccgc caccaaagag aatagaaaat atttctctaa aattttcatt
     2041 aatctcctta aattgcttta caaagattgt tctcatcttt ttttccatat caataattat
     2101 ctcttctaac ctttccttgc tactggttag atcctctttt tgctcaacta aaaagtctat
     2161 cctttccttg accttatcat actcatctaa gg
//
LOCUS       DATGSI010000111         1160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_481179, whole genome shotgun sequence.
ACCESSION   DATGSI010000111 DATGSI010000000
VERSION     DATGSI010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481179"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VK031_01650"
     CDS             <1..628
                     /locus_tag="VK031_01650"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HLS52653.1"
                     /translation="MLIHYIIAILVMGLSLFFNLSRIEFLILLFATTLVVVTELINTA
                     IEKTVDMITKDFHPVAKIAKDIAAGAVLVAAINAVMVGYLIFYDRLNDFTKLTLVKIK
                     NSPIHLTFVALILTTLLTIGLKAKFYTGRGTHFQGGAVSGHSALAFCIATIIGSLGRN
                     VLIISLSYLLAGLVAESRIEGKIHTPIEVVLGAMLGLIIGILIFQVIG"
     gene            659..1069
                     /locus_tag="VK031_01655"
     CDS             659..1069
                     /locus_tag="VK031_01655"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HLS52654.1"
                     /translation="MKKKDLIKKAIEAKKKAYTPYSNFQVGAAVLTEDGKIYTGCNIE
                     IASYSPTICAERTAIFKAVSEGSKKIQAIAVTGDASPTYPCGVCRQVIREFGKDAVII
                     IADSEDKFKEYSLDELLPFSFGPKDLNKEEKKGV"
     gene            1062..>1160
                     /locus_tag="VK031_01660"
     CDS             1062..>1160
                     /locus_tag="VK031_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HLS52655.1"
                     /translation="MYKSGFISIVGRPNVGKSTLLNQVIGEKISIIS"
BASE COUNT          380 a          172 c          241 g          367 t
ORIGIN      
        1 tatgttaatc cattatatta ttgctatttt ggttatgggc ctaagcctat tttttaatct
       61 ttctagaata gaatttttaa tccttttatt tgctactacc ttggtagtgg tgacagaatt
      121 aataaatacg gccatagaaa aaacagtaga catgataact aaagattttc atccagtggc
      181 taaaatagct aaagatattg cagcaggagc tgttttggtg gcagcaataa atgctgttat
      241 ggtaggatat ttaatctttt atgataggtt aaatgatttt accaagttaa ccctggttaa
      301 gataaagaac tctcctatcc atctaacctt tgtagccctt attttaacaa ccctactgac
      361 cataggctta aaggccaaat tttacactgg acggggtaca cactttcaag gcggggcagt
      421 tagtggacat tctgctttag ctttttgtat agcaactatt atagggtctt taggcaggaa
      481 tgttttaatc ataagcttat cctacctttt agcaggcctt gtggcagaaa gcagaataga
      541 gggaaaaatt catacaccta tagaggttgt tttgggagcc atgctaggtt tgattatagg
      601 tatattaata tttcaagtaa ttggttaatt aaattagaag gctacttggg aggttaaaat
      661 gaagaaaaaa gatttaatta aaaaggctat agaagcaaaa aagaaggcct acactccata
      721 ttctaatttt caagtagggg ctgcagtttt aactgaagat gggaaaatct ataccggttg
      781 taatattgag atagcttcct attctcctac tatttgtgct gaaaggacgg ccatatttaa
      841 ggcagtatct gaaggaagca aaaaaattca agccattgct gtgacagggg atgcaagccc
      901 tacctatccc tgtggtgtat gcaggcaggt tattagagaa tttggtaagg atgcagtcat
      961 tattatagct gattctgaag ataagtttaa agagtatagc ctggatgagc ttttaccctt
     1021 tagctttgga cctaaagatt taaacaagga ggaaaagaaa ggtgtataaa tcaggattca
     1081 ttagcatagt tggaaggcct aatgtgggaa aatccacatt acttaatcag gtaatagggg
     1141 aaaaaatctc aattatttca
//
LOCUS       DATGSI010000112         1788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_575849, whole genome shotgun sequence.
ACCESSION   DATGSI010000112 DATGSI010000000
VERSION     DATGSI010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575849"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VK031_01665"
     CDS             complement(<1..380)
                     /locus_tag="VK031_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HLS52656.1"
                     /translation="MKKANSFTIRKMVLVGMLGAISIVLGMTPLGFIPIGPTRATIMH
                     IPVIIGAITAGPLVGGLVGLIFGLFSIFQAITNPTPVSFVFLNPLVSVLPRILIGLVT
                     YYIYEGLKRLGDKNTKLLLYLIWI"
     gene            complement(502..1470)
                     /locus_tag="VK031_01670"
     CDS             complement(502..1470)
                     /locus_tag="VK031_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HLS52657.1"
                     /translation="MHKGYITDIEGLKVGHSQCEQGLTGCTVIIAEEGATGGVDVRGA
                     APGTRETDLLSSEKMIEQIHAVVLAGGSAFGLEASSGVMNYLEESNVGFDVDVTRVPI
                     VTSAVIFDLNIGDPKIRPDYKMGYEAAKTASSKERRQGNIGCGMGATVGKILGPDGAM
                     KSGLGSATVTAGELKVSALVVVNSFGDIFDYRNNKQIAGVYDYGSKKLLNTYQIMKEE
                     NKALKFSMTNTTIGLVATNAVLSKAEANKIAQMGHNGFARSIDPIHTMLDGDTIFTMA
                     TNKIKADINLVGSLAAEAVSQAISNGIYNAEGIEGLYAFRDIKGLD"
     gene            complement(1564..>1788)
                     /locus_tag="VK031_01675"
     CDS             complement(1564..>1788)
                     /locus_tag="VK031_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HLS52658.1"
                     /translation="LIKDNMDILERIAQALLEKETLDAKEFEEVFLGRKLEEGEYTEY
                     DRISDGIDSNETTSNNTVDNTDENEKNKED"
BASE COUNT          505 a          411 c          255 g          617 t
ORIGIN      
        1 cttatccaaa ttagatataa taaaagctta gtatttttgt ctcccaatct ttttaatccc
       61 tcatatatat aataggttac caggcctatt aatatccttg gtaaaactga taccaaagga
      121 ttcaaaaaaa caaaagaaac aggagttgga tttgttatgg cctgaaatat gctaaatagg
      181 ccaaatatta aacctaccag gcctcctaca agaggaccag cagtaatagc tcctataata
      241 actggtatat gcataatagt agctctagta ggtcctatgg gaataaaccc taagggtgtc
      301 atccctaata caatggatat ggcccctaac ataccaacca agaccatctt tctgatggta
      361 aaactatttg cttttttcat tttatccctc cgctctagtt cctatccagg atactagtcg
      421 atattttaat ttaactctgt tagtccagct cccttggata ctggctactt tgacattacc
      481 attataaaat catatataag gctagtcaag accctttata tctctaaagg catatagccc
      541 ttctataccc tcagcattat atataccatt ggaaattgcc tggcttacgg cttctgcagc
      601 taggcttcct accaagttta tatctgcctt tatcttattg gtggccatgg taaagatagt
      661 atctccatct aacatggtgt gtattggatc tatggaacgg gcaaatccat tgtgccccat
      721 ctgtgcaatt ttattggcct cagccttact taatacggcg ttggttgcca ccagcccaat
      781 ggttgtgttg gtcatggaaa attttaaggc cttattttct tccttcatta tttggtaggt
      841 attaagaagt ttcttgcttc cataatcata tacccctgct atttgcttat tattcctata
      901 atcaaatata tccccaaaac tatttactac tacaagggct gataccttta attctccagc
      961 tgtaactgtt gcactgccta aacctgattt catagcacca tcaggtccta atattttccc
     1021 cactgtagcc cccataccgc agccaatatt gccctgtctc ctctccttac tagaggcagt
     1081 ctttgctgct tcatagccca ttttataatc tggtctaatt ttgggatctc ctatatttaa
     1141 atcaaatata accgctgatg taactattgg cacccttgta acatctacat caaagcctac
     1201 attgctttcc tctaaataat tcattacacc agatgaggct tctaatccaa aggcagaacc
     1261 tccagccaag actacagcat gaatttgttc tatcattttt tcagaactta atagatctgt
     1321 ctcccttgta ccaggggctg cccctcttac atctacacct ccagtggctc cctcctcagc
     1381 aataataact gtacaaccag ttaatccttg ttcacattgg gaatggccta cctttaggcc
     1441 ttcaatatct gttatataac ccttatgcat taaaagcacc tctttcctaa tatactccta
     1501 gggtaaagtt ttaatgaagt tacatttagt aattaaaaaa aggtaggata cacctacctt
     1561 cttttagtct tctttgtttt tctcattttc atcagtatta tcaactgtat tattggaagt
     1621 tgtttcattg gaatctatac catcagatat tctatcatat tctgtatatt ctccttcttc
     1681 caactttcta cctaggaata cttcttcaaa ttctttagca tccaaggttt ctttttctag
     1741 taaggcctga gctatcctct ctaatatatc catattatct ttaattaa
//
LOCUS       DATGSI010000113         1696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_151332, whole genome shotgun sequence.
ACCESSION   DATGSI010000113 DATGSI010000000
VERSION     DATGSI010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151332"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VK031_01680"
     CDS             <1..1458
                     /locus_tag="VK031_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52659.1"
                     /translation="KAWLTGDMAKYLIGTAFHKLGWKTSKDIEEFVKAFGLTGMSQSV
                     DRSNLVRGAIMEAVDRRNIFTKSVGKATEFTRTIGHDAGEKFNLLGHYAAVWSKYKRR
                     GDDLTDPYVLSKLHSEARAISQDYNVAGDFAYTQNILSVPLQFAQVLHKFLLTPFNRR
                     YSRAERWRMVAGDLILWGIPGTYTVSELIGFDILPEDPEWRERFAEGVTSWGINNLLN
                     AVFKDDPAVSLESTAPYNIGSFIELMKSSITDGWGEMIFNTPSGQLLFKSEGRVGMAF
                     RSLFRHFAPISEGMESPETILDTVNQFAKISSGWSNITKAWAQAQTGIAVDRLNLPTD
                     TNVKLSEAIAQAFGFPTKTTRDYYRTIERTINRKEEVEKVARDIANSIFEMYSVAYSG
                     DSAVDPEYTTRLTGSILHLAKKNPLLAARVYDLIKMRLNDPEDSTMHNILKAINMPTV
                     ENLERDIRDSGLPEDKKKQLLDILATRNRIINEDE"
     gene            1459..>1696
                     /locus_tag="VK031_01685"
     CDS             1459..>1696
                     /locus_tag="VK031_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52660.1"
                     /translation="MATTFGVDATSLQEPREAGAQVIEGVRRAPSQYSLPTGLLDIGV
                     QVVRDVHQREQKELQAAAEQRDRQLIADYTRAQTR"
BASE COUNT          552 a          312 c          389 g          443 t
ORIGIN      
        1 aaggcatggc ttacagggga tatggctaaa tatcttatag gtactgcctt ccataaatta
       61 ggctggaaaa catccaaaga tatagaggag tttgttaaag cctttggatt gactggtatg
      121 tctcaatcag tggataggtc taacctcgtc agaggcgcta taatggaggc cgtagaccga
      181 agaaacatat tcactaagtc tgttgggaaa gctacagagt ttactagaac tattggccac
      241 gatgctgggg aaaagtttaa cttgttgggt cactatgccg ctgtatggag caagtataaa
      301 agaagagggg acgatctaac tgacccttat gttttatcta aacttcatag cgaagcaaga
      361 gctatatctc aagactataa cgtagcaggg gattttgcat acactcagaa tattttatct
      421 gttcctctcc agtttgcaca ggttcttcat aaattcttat taactccctt taacaggaga
      481 tattctagag cagagagatg gaggatggta gctggggatc taatcttatg gggtatacca
      541 ggaacttaca ctgtctctga gttgataggg tttgacatac ttccagaaga tcctgagtgg
      601 agagagagat tcgcagaggg cgtaacttct tggggtatta acaacctact gaacgctgtg
      661 tttaaagacg accctgctgt atctctggaa tccacagccc catataatat cggaagtttt
      721 atagaactaa tgaagtcatc cattacagat ggttggggag agatgatatt taacactcct
      781 tctggacagc tgttgtttaa atctgaagga agagtaggta tggctttccg aagtttattt
      841 aggcatttcg cccccatatc agaagggatg gagtccccag aaactatttt agacaccgtt
      901 aaccagtttg ctaaaatctc gtccggttgg agtaacataa ctaaggcttg ggctcaagct
      961 cagacaggaa tagcagtgga taggttaaac ctccccacag atactaatgt aaagctatcc
     1021 gaagcaatcg cacaagcgtt tggatttcct accaaaacca caagagatta ttaccgaact
     1081 atagaaagaa ctattaacag gaaagaagaa gtagagaagg ttgctagaga tattgctaac
     1141 agtatctttg aaatgtactc tgtggcatat tcaggagaca gtgcggtaga tccagaatac
     1201 actacaagac tgacagggtc aatacttcat ttagctaaaa agaatccttt actcgcagca
     1261 agagtatatg atttaataaa aatgaggctc aatgacccag aagactctac tatgcataat
     1321 attcttaagg ctataaatat gcctactgtt gagaatttag aaagagatat tagagattca
     1381 ggcttaccag aggataagaa gaaacagctt ctggatatct tagctactag aaatagaatt
     1441 attaatgagg acgaataatt ggctactact tttggagtag atgccacatc tttacaagag
     1501 cctagagagg caggggcaca agtaatcgaa ggagttcgaa gggctccttc tcaatactcc
     1561 ctgcctaccg gcctattaga tataggtgta caggttgtac gggatgtgca tcaaagagag
     1621 caaaaagaac tacaggcagc ggctgagcaa agagacagac aactaatagc agactacact
     1681 agggcccaaa ccagag
//
LOCUS       DATGSI010000114         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_431775, whole genome shotgun sequence.
ACCESSION   DATGSI010000114 DATGSI010000000
VERSION     DATGSI010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431775"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VK031_01690"
     CDS             <1..245
                     /locus_tag="VK031_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HLS52661.1"
                     /translation="LILIVAKTPQIILKLVEGGVPIDKVNIGNMHFSEGKKQITSTVS
                     IDEEDAKTFRRLDELGVKLEIRRVPSERATNILEFI"
     gene            299..1066
                     /gene="agaW"
                     /locus_tag="VK031_01695"
     CDS             299..1066
                     /gene="agaW"
                     /locus_tag="VK031_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HLS52662.1"
                     /translation="MFLKALLVAIWAGLAGVDMFDGLTHFHRPVFTGMVVGLILGDVK
                     TGLITGATLEFVWMGAVPLAGAQPPNVVIGGIIGSAFAILANQDPQVAVGIAVPFAVA
                     VQGAITLFFTIFSPVMHKADEYARDADTKAIERINYLAMGILFIFYAIVAFLPVYFGA
                     ETAQGIVESMPAWLISGLEVAGGMMPAIGFAMLLNIMLKKEYVAFLIIGFVLVAYFEL
                     PILGVSLIAVAIAMYDYYSGQRRQVQTSRGGGRTDGI"
     gene            1056..>1549
                     /locus_tag="VK031_01700"
     CDS             1056..>1549
                     /locus_tag="VK031_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HLS52663.1"
                     /translation="MESNVGYVDQTAEKVITQRELNRMTWRSLFLQASFNYERMQACG
                     WLFGLIPGLKKIHKDKDDLGQSMSMHMEFFNTHPFLVTFIMGIVIAMEENKEHPDAIR
                     AIKVATMGPLGGIGDALFWMTLLPISAGIGASMAMEGNIAGPFVFLAIFNVVHFALRF
                     GLMN"
BASE COUNT          475 a          239 c          381 g          454 t
ORIGIN      
        1 aattgatttt aatagttgcg aaaactccac aaattatatt gaaactggtt gaaggtgggg
       61 taccaattga caaggtaaat attggaaaca tgcatttcag tgaggggaaa aagcaaataa
      121 ccagtacagt ttccatagat gaggaagatg caaaaacatt taggaggctg gatgagttag
      181 gtgtgaagtt ggagatcaga agggtaccta gcgaaagggc tacaaatatt ttagaattta
      241 tttaggtcag taaatcttgc aatgcaagat tattaaataa acaaaggggg taaaatatat
      301 gtttcttaaa gctttgctcg tagcaatatg ggccggcttg gcaggagttg atatgtttga
      361 tggattaact cacttccata ggccagtatt tacaggaatg gtagtagggc taatcctagg
      421 agatgtaaag actggtctta ttacaggggc cacattagaa tttgtatgga tgggggcagt
      481 gcccttagca ggtgcgcagc caccgaatgt ggttataggt ggaattatag gatcggcttt
      541 tgccattcta gcaaaccaag atccgcaagt ggcagtggga atagcggtac cttttgcggt
      601 ggcagtacaa ggtgctataa ccttattctt tacaatattt tcaccagtta tgcacaaggc
      661 tgatgaatat gcaagggatg ctgatacgaa ggctattgaa agaatcaact atttagcaat
      721 gggaatattg ttcatatttt atgcaatagt agctttttta ccagtatact ttggtgccga
      781 gactgcacaa ggaatagtag agtccatgcc tgcatggtta atctcaggat tagaggtagc
      841 tggcggtatg atgcctgcca tagggtttgc aatgttgttg aacattatgt taaagaagga
      901 atatgtggct tttctaatca ttggatttgt attagtagca tattttgaac taccaatctt
      961 aggtgtatca ttaatagctg ttgcaatagc catgtatgac tactatagtg gtcagagaag
     1021 gcaagttcaa acttcaaggg gagggggacg aactgatgga atctaatgtt ggttatgtag
     1081 atcaaactgc tgagaaagtg ataacccaga gagagctcaa tcggatgaca tggagatctt
     1141 tatttctgca ggcttccttc aactatgaaa gaatgcaggc ttgtggatgg cttttcggac
     1201 taattccagg tcttaagaaa atccataagg ataaagatga tttgggacag tctatgtcta
     1261 tgcatatgga attttttaac actcacccat tcttggtaac gtttatcatg ggtatagtaa
     1321 tagccatgga agagaataag gaacacccag acgccataag agccataaag gttgcaacca
     1381 tgggaccttt aggcggaata ggagatgcac tattctggat gaccttacta cctatcagtg
     1441 ccggaatagg agcctctatg gcaatggaag gaaatattgc tggtcccttt gtattcctag
     1501 ctatatttaa tgtggttcat tttgcactaa gatttggtct tatgaacta
//
LOCUS       DATGSI010000115         1103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_290191, whole genome shotgun sequence.
ACCESSION   DATGSI010000115 DATGSI010000000
VERSION     DATGSI010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290191"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VK031_01705"
     CDS             <1..747
                     /locus_tag="VK031_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52664.1"
                     /translation="ISNNYSVKQYNIPRERERVTTVSRHKNNNKRLKTSTLDDLIAGV
                     DFREKVVVDLGMKVKVAKVGVNALKVPVGSRNSSLFFMSCQIKALNEWAQFEDIIPYM
                     REVNRKGMRVPVSESELIKIVNNALDKYSRGDLKIVYNSSRRFLFNPEFELTAREKRS
                     IVMKIINETRKANTKRRVYRYIRREKTTHKKLISERLKLSLKTTAEYVNKYLIEEMFK
                     KEGEVCYKIVAERVGIKEHTAKNYIKELGL"
     gene            791..1066
                     /locus_tag="VK031_01710"
     CDS             791..1066
                     /locus_tag="VK031_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52665.1"
                     /translation="MKKKVNKTGDWGKFHDPSTLVEFIHMSSTAGLVMSSYFNDKGDL
                     HPELLKYLGVKSGSEGEKAFYEVIKQLQEETAKVMVTGFQIVPPSFE"
BASE COUNT          426 a          121 c          250 g          306 t
ORIGIN      
        1 atttctaata attatagcgt aaaacagtat aacataccta gagagaggga gagggttacg
       61 actgtgtcac gtcataaaaa taataataag agattgaaaa ctagtacttt agatgacttg
      121 atagcaggag tagattttag agaaaaggtc gttgtagacc ttgggatgaa ggttaaggta
      181 gctaaagtgg gtgtgaatgc gcttaaagtt ccagtaggta gtaggaatag ctcattattt
      241 ttcatgagtt gtcaaataaa agctttaaac gaatgggcgc aattcgagga tataatacca
      301 tacatgaggg aggttaatag aaaaggaatg agggtaccag tatcggaatc agagttaatt
      361 aaaattgtta ataacgcttt agataaatat agtagaggag atttgaaaat agtttataat
      421 tcttccagaa ggtttttatt taatccagag tttgaattaa cagctagaga aaaaaggtca
      481 atagtaatga agataattaa cgagactaga aaggctaata ctaagagaag agtttacagg
      541 tatatcagga gagagaaaac aacacataag aagttaatat ccgaaaggct taaactatcc
      601 cttaaaacca ccgctgaata cgtaaataag tatttaatag aagagatgtt taaaaaagaa
      661 ggagaggtgt gttataaaat tgttgcagaa agggttggta taaaagagca tactgctaag
      721 aattatataa aagaacttgg tttgtaaaca aaaaagtgtt atatttgtgg aataatataa
      781 agaattagat atgaaaaaga aggttaataa aacaggtgat tggggtaagt ttcacgaccc
      841 atctacttta gttgagttta tacatatgtc atccacagca ggattggtga tgtcgtctta
      901 ttttaacgat aagggagact tacaccctga actattaaag tacttaggag ttaagtctgg
      961 ctctgagggt gaaaaggcat tctatgaagt tataaagcaa cttcaagaag agactgctaa
     1021 ggtgatggta acaggatttc agatagtacc tccttcgttc gaatgattag gaaatataag
     1081 aggaagggtg gtaatactaa agc
//
LOCUS       DATGSI010000116         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_622637, whole genome shotgun sequence.
ACCESSION   DATGSI010000116 DATGSI010000000
VERSION     DATGSI010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622637"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..985
                     /locus_tag="VK031_01715"
     CDS             239..985
                     /locus_tag="VK031_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HLS52666.1"
                     /translation="MDVVKYLKRIGVDKDIKVDLESLAYLQNQHLLTVPFENLDVINK
                     VHIPLDVKTYYEKIVNNNRGGFCYELNGLFHWLLGKLGFSSYLVAATLYRGEGNWGRS
                     NSHASLIVDLNRPYLVDVGFGNSARKPLPLNGGERKDVSGLYRVIPAKEGYYYKQRKE
                     GGNWRTLYRFLVEERKLSDFEKQCHFVQTSPTSHFTQRRLVTIATEKGRKTLSKDKLT
                     IVENGERREIEVKDWKLASLLAKDFGILDQ"
     gene            complement(1232..1648)
                     /locus_tag="VK031_01720"
     CDS             complement(1232..1648)
                     /locus_tag="VK031_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52667.1"
                     /translation="MNIKKEQTTHIILLVTGIFILIGGPLIVKMAQLDTFLIGGVSGF
                     GGAWTVNSAIKLYQIRKKPEEYEKQVIEQYDERNIAIRGYAGYATFITTLLTISVMSV
                     IFMVLDYTRPLLIGGILLLIHIVSFLVYFRYYSKKL"
     gene            complement(1645..1848)
                     /locus_tag="VK031_01725"
     CDS             complement(1645..1848)
                     /locus_tag="VK031_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLS52668.1"
                     /translation="MKNNLEKLRREKGISQEELANNLKVSRQTISSIENGRYNPSIIL
                     AFKIARYFNMKIEDIYIYEGEER"
     gene            2107..2462
                     /locus_tag="VK031_01730"
                     /pseudo
     CDS             2107..2462
                     /locus_tag="VK031_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          856 a          408 c          391 g          807 t
ORIGIN      
        1 tagatctaaa gcttcagcag ctccactaat agtttctacc ttatcaattg tttgattgat
       61 aactctaagt ctgttaattt ctttttgagt catattatat atttcctttc tcatactgac
      121 attttatcag aataatttta atatgacatt atcatagaat aaacacacac ttaacggttc
      181 ttgacatttg ggctattctt gctagactaa tgtttaaact ttaaaaaggg gtgtcaaaat
      241 ggatgtagtt aagtatttaa aacgtatagg cgtagataaa gatattaagg tagatttaga
      301 atctttagcc tatttacaga accaacattt attaactgta ccctttgaaa acttagatgt
      361 tataaacaag gtccatatac cactagatgt gaaaacctac tatgaaaaga ttgttaataa
      421 caaccggggt ggcttttgtt atgaactaaa tggacttttt cattggttgc taggtaaact
      481 tggtttttcc tcctaccttg ttgcagctac cctctatagg ggagaaggta attggggcag
      541 atctaatagt catgccagtc taatagtaga tttaaatagg ccttatttag tagatgtagg
      601 ctttgggaat tctgctagaa agcccctccc cttaaacgga ggtgaaagaa aggatgtaag
      661 tggcctgtat agggtaattc cagcaaaaga aggctactat tacaagcaaa gaaaagaggg
      721 tggaaactgg agaacccttt atagattttt agttgaggaa agaaaactaa gtgattttga
      781 aaaacaatgt cactttgttc aaacctctcc cacatcccat tttacacaaa gaagattggt
      841 tactattgca acagaaaagg ggcgtaaaac cttatcaaaa gataaattaa ccatagtaga
      901 aaatggggaa aggcgagaga ttgaggttaa agattggaag ctggcatctt tgttggctaa
      961 agattttggg attcttgatc aataatctgt ataaatatat ttttatccac attaatttta
     1021 aagttatagt taacatatcc acaattaatc caggtttttt aacttaagta ctattactgt
     1081 caacatcctt ataatttact actgataggg taacaagaac ctttcatttg ccttaaaggt
     1141 aaaagatggc aaagcttaag tccatctaac ctaaacttta ccatctttta tcttattatt
     1201 ttaccttatt ttatcatagg ggatctacac tctacagttt tttgctgtaa tatctaaaat
     1261 aaactaaaaa actaacaata tgaatcagta atagtatacc tccgattaaa agaggtcgtg
     1321 tatagtctaa aaccataaat ataacagaca taacacttat tgttaataat gtagtaataa
     1381 atgtagcata tccagcataa cccctgattg ctatattccg ttcatcatat tgttctatta
     1441 cttgtttctc atattcttct ggtttttttc taatttggta tagcttaata gcactgttta
     1501 cagtccatgc accaccaaaa ccacttactc cccctatcaa aaaagtatct agctgtgcca
     1561 tctttactat aagaggtcct cctatcaata tgaaaatacc ggttactagt agaataatat
     1621 gggtggtttg ctcttttttt atattcatct ttcctccccc tcataaatat atatatcctc
     1681 aatcttcata ttaaagtacc ttgcaatttt aaaagctaga attattgacg ggttatatcg
     1741 gccattttcg attgaactaa tagtttgccg tgaaaccttt aaattatttg ccaactcttc
     1801 ttggctaatt cccttctccc ttcttagctt ttctaaatta tttttcacaa tataccccac
     1861 ctaacatttg taaagttcac tttacatcta tataaattat aactacctat tagccttatg
     1921 tcaagtaagc tttacatctt ttgtaaacaa aaacctactt ttttaataat aagaaagctc
     1981 tggtaaggcc ttgtggctac aaattataat taacataaaa aggactctaa attatattgg
     2041 ataggttaca ctaaatagga cagatcttta cttaacatga tagaataaaa tcaggaggag
     2101 attttaatga ccagaaagcg atatgatgaa gattttaaaa aacagatagt aaagatctat
     2161 aaccaaggga atcacagtta cagaagccta gcaggtgaat atggcgtagc tgcccctaca
     2221 attagaacct gggtattaag atataataat tctaattcct ttgatgtaga agataataga
     2281 actgaagagg aaaaagaatt aattgagctt agaaaaaaag taaagcaact tgaaatggag
     2341 aatgatattt gccctaaagg ataagctcca ctaaggcaat aaattgccaa gtgagtcgct
     2401 taaaagcagg cagcactact actaggcaaa aagtagacct tatcatttct aatagagaga
     2461 aa
//
LOCUS       DATGSI010000117         9269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_151504, whole genome shotgun sequence.
ACCESSION   DATGSI010000117 DATGSI010000000
VERSION     DATGSI010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151504"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VK031_01735"
     CDS             complement(<1..325)
                     /locus_tag="VK031_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HLS52669.1"
                     /translation="MDKNLFHWSDNKIYLIITGILVLIIGIYQPVLGMIGALVFGYLI
                     YYTFIETQNKNKEVDKYIEALSGEFESATKHAIFNMPFPLVIIDEQGTITWYNTPFLN
                     MVVEEE"
     gene            complement(335..1273)
                     /locus_tag="VK031_01740"
     CDS             complement(335..1273)
                     /locus_tag="VK031_01740"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HLS52670.1"
                     /translation="MRNNQINRKIKESLILITGITFLIVLGIAYTPLVLLFPAVYSIF
                     ALRNGINLGITNLIITGILTGIMVGVELAVSLLIFVFPMTFVMTYLIKKRRKSIEVLG
                     ISTVTFFILVILLVKLMEIKGINFIAILEESFSQAIMTQLELYQDMGLTSLELLERRD
                     LLEGAYKQVLLIMPSIILIVSLVINYINYLLTTYVLDKMDIKLHKVPKFSRFKLPNNI
                     ILGILAIFIGFYASKYIGFQYYEETFINLNVLIGVAFFIQGLAVIQYTFNRRKINHLI
                     RIILYVIFLFIAPVITIIGIIDTIFDIRKLRGSRSL"
     gene            complement(1291..1608)
                     /locus_tag="VK031_01745"
     CDS             complement(1291..1608)
                     /locus_tag="VK031_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HLS52671.1"
                     /translation="MEVNKNIDIIKNMKTVEWLKAELLTNIAYLHKVLVNNEDNTRED
                     LEDIISNIILETYILGKRLGLEYENIDQALKENIKINLIQEHKIEKWYGDLSKLLNHI
                     DSR"
     gene            complement(1709..1933)
                     /gene="rpsR"
                     /locus_tag="VK031_01750"
     CDS             complement(1709..1933)
                     /gene="rpsR"
                     /locus_tag="VK031_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HLS52672.1"
                     /translation="MQRRFRPRKRVCSFCAEKSERIDYKDINKLKKYVTDRGKILPRR
                     ISGNCSKHQRELTTAIKRARQVALLPYSAD"
     gene            complement(1949..2383)
                     /locus_tag="VK031_01755"
     CDS             complement(1949..2383)
                     /locus_tag="VK031_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HLS52673.1"
                     /translation="MNNVVLIGRLVADPDLRYIPSGNAVSRFTLAVDKQLSREKKQEL
                     EARNQPTADFIRIVVWGKQAENCANYLAKGRLVAVQGRIQTGSYEDKDGKTVYTTDVV
                     ANNVQFLEWGDPRSNDAGFEAQDSDFPGIDGLHPVDNDDIPF"
     gene            complement(2406..2687)
                     /gene="rpsF"
                     /locus_tag="VK031_01760"
     CDS             complement(2406..2687)
                     /gene="rpsF"
                     /locus_tag="VK031_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HLS52674.1"
                     /translation="MRKYEAVFIFLPNEEEESRNQLLERFKSIIKEDGEVTNVEEWGV
                     RKLAYLINDIGEGYYVIVNFQGTPETVNELDRIAKISDSIMRHMIIRED"
     gene            complement(2839..3312)
                     /locus_tag="VK031_01765"
     CDS             complement(2839..3312)
                     /locus_tag="VK031_01765"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HLS52675.1"
                     /translation="MKIFSYILKKDPQDYNTRAQIMLAGSLAHSGLLGLGREEDWASH
                     FIVHEIGALYDETHGITLSIVFPVWMKYVYKTNLDRFVQFAKRVFQVSTDGKTREDIA
                     LEGIYRFEGFLKELGLPTSFKEAGLPTDKIHLMAEKVTENGPLGRFKKTGKRGCN"
     gene            complement(3430..3654)
                     /locus_tag="VK031_01770"
     CDS             complement(3430..3654)
                     /locus_tag="VK031_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004822154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HLS52676.1"
                     /translation="MLHYKVGDIITLKKGHPCGENKWEILRTGVDIKLKCLGCDRQIW
                     LTRINFEKRVRKILVDEKWISIVHHKPEES"
     gene            complement(3654..4508)
                     /locus_tag="VK031_01775"
     CDS             complement(3654..4508)
                     /locus_tag="VK031_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HLS52677.1"
                     /translation="MENINYLVETYLKDNIGNLNILGRLIKIILIFVVIWLVNRIANK
                     VIDKTLSTRKTKSTTIDDKRANTLALVLKKLVYYILTFIGIMMVLDMFNVPTTSILAT
                     AGIGGLAIGFGAQSLVKDIITGFFILLEDQYSVGDLIETGDFEGVVEELGLRVTKLRA
                     FSGELHIIPNSSIQTVTNKTRGAMRALVQVTIAYEENIDRAMEVLNKACERLKKENKA
                     IVEGPVALGVAELAEYGFVINIIARTKPMEQWAVERAIRKMVKETLDKEGIEIPYPKR
                     IIIGGEKE"
     gene            complement(4524..4790)
                     /locus_tag="VK031_01780"
     CDS             complement(4524..4790)
                     /locus_tag="VK031_01780"
                     /inference="COORDINATES: protein motif:HMM:NF023251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HLS52678.1"
                     /translation="MKREEYGLITFKTTHFALEAERLFKNEHITCKTIPTPREVSHSC
                     GLSLLFMMDDLARVQAIIDKGQITIDGLFGYTKVNHKNLFERLI"
     gene            complement(4803..5390)
                     /gene="yedF"
                     /locus_tag="VK031_01785"
     CDS             complement(4803..5390)
                     /gene="yedF"
                     /locus_tag="VK031_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HLS52679.1"
                     /translation="MIEIDARGQACPKPVIMTKQKLDEINQGLVVTIVDNEVAKENVS
                     KLAQGMGLSSIVEKKGNDYYISIEKGDREAGVETNIGYELEDITIGISSNTMGKGDER
                     LGEILIKSFIYTLSETKPYPSTLVFYNGGVRLTTEGSEVLDDLKKLEDAGVEIISCGT
                     CLDFLNIKDKLEVGSISNMYTIYERLRESEKNIII"
     gene            5653..6021
                     /locus_tag="VK031_01790"
     CDS             5653..6021
                     /locus_tag="VK031_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HLS52680.1"
                     /translation="MNAKVIGKCPVCNHNLKVTRLSCNNCHTSIEGNFDLCKFSRLSK
                     EQRAFLEVFVKNRGNIRDIERELNISYPTVKSRLDNLIESLSYNPKDSEPAISRRDVL
                     KRLENGQITPKEAVKLLKGE"
     gene            6042..6407
                     /locus_tag="VK031_01795"
     CDS             6042..6407
                     /locus_tag="VK031_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52681.1"
                     /translation="MPEKLQILNMVSEGKISAEEGMELLSALDEPKQDGRDRQKRRSL
                     RVKIHGPSEDAKVNATIPISLIRLGFKLASKLSPEFREAGLAEIDLEEIIEAIDDGAV
                     GTIVEVDADDGTKIEVIAE"
     gene            6503..7957
                     /locus_tag="VK031_01800"
     CDS             6503..7957
                     /locus_tag="VK031_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HLS52682.1"
                     /translation="MLKLSVDKDSTTPLYVQIYDSIKQLIEEGKIKGDKLPSIRSLAK
                     SLNVNNVTIVNAYKLLEQNDYVYSIKGSGTYIKRTLNPEEFSYLEEGDMELMVSGVLP
                     ISDNSINFASVSPTPDLFPINEFKQALVQVLDRDGGLAFLYPEVTGYEPLRESISKFL
                     FINYKTRVNKDRILITSGGQQGLDIISKTLINQGDLILVENPTYLGALAAFESRGARV
                     VGIPMMEDGIDVDLLESYVKKHNPKFLYMMTNYQSPTTYSYSQEKKIKVIELANRYNF
                     YIIEDDFLTDLSFNCEMKTPLKSIDKNDKVIFIKSFSKIFMPGIRIAFVTVPNILFRD
                     VIKAKHTTDVSSSGFLQRAFDQYLRTGYWKQHINKVKKVYKERYELITREIDRLKVYG
                     INYLPPKGGLSLWLSLPKHIDAIQLYQQCNEENLALVPGKVFFINDDLNTNHIRLSFG
                     NVSNEEIIKGISILEDIIRKQSSSHTTSKYLPFV"
     gene            complement(7975..8994)
                     /locus_tag="VK031_01805"
     CDS             complement(7975..8994)
                     /locus_tag="VK031_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52683.1"
                     /translation="MNRNWIKIASIYIGTVIGAGFASGREILDFFAIYGAKGIWGIYI
                     AGLLFAAVGSLLLYKIHKGQVKNFDQLNQDVFGKKLAPLVDALTILSLYTGFSVMVAG
                     SGAVFKEQLGLPFYMGVVVMLVCCFIVFLFNLEGLSLINTILVPLLIIGILFTSHYFS
                     SKTGYKFPYLDGISFTLKGNFISSAILYFGSNSLLIIVVFSSLGDLIDSRRTAILGGL
                     TGGFILYILGMAILYSILLHFIEIQYVDVPMLKISNYISKDYGKIYSLILWIAMFTTA
                     LANGFGFVNRLGRSRNRIIVIVIFSISTIPLARLGFAKLIGIIYPVFGLIGSIMIFRI
                     IISNK"
     gene            complement(9132..9248)
                     /gene="rrf"
                     /locus_tag="VK031_01810"
     rRNA            complement(9132..9248)
                     /gene="rrf"
                     /locus_tag="VK031_01810"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2945 a         1753 c         1389 g         3182 t
ORIGIN      
        1 tttcttcctc tactaccata tttaaaaagg gtgtattata ccaggtaata gtaccttgtt
       61 catctattat gaccagcgga aaaggcatgt taaaaatagc atgcttagta gcagactcaa
      121 actctccaga taaggcttca atgtatttgt caacttcttt atttttattt tgggtctcta
      181 taaaagtata ataaattaag tatccaaata caagggcccc aatcatacct aaaactggtt
      241 ggtaaatccc tattataagc accaatattc ctgttataat caaatatatt ttattatctg
      301 accaatgaaa taaattttta tccataatat cctcctacaa ggatctagag ccccttagct
      361 ttctaatatc aaatatggta tctataatac caatgatagt aatcaccggt gctataaaca
      421 aaaatataac atataggatt attctaatta aatgattaat tttccttcta ttaaaagtat
      481 attgaattac tgctagtccc tgaataaaaa aggcaactcc tattaagaca tttagattta
      541 taaaggtctc ttcataatat tgaaaaccta tatatttaga tgcgtaaaat cctataaata
      601 tggcaagtat gcccagtata atattgttag gtaacttaaa tcttgaaaac ttaggcacct
      661 tgtgtagctt tatatccatt ttatccaaca cataggtggt taaaaggtag tttatataat
      721 ttattactaa agaaacaatt aagattattg agggcataat taacaagact tgtttatagg
      781 ccccttctag caggtctctt ctttccaata attctaagga tgtcagcccc atatcctgat
      841 atagctccaa ttgggtcatg attgcctggc tgaaggattc ttctaatatg gctataaaat
      901 tgatgccctt tatttccata agctttacta ataatattac caagataaaa aatgtaacag
      961 tggatattcc taatacctct atacttttcc ttctcttctt aattaaatag gtcataacaa
     1021 aggtcatggg aaaaacgaat attaataaag atacggctaa ttccacgcct accataattc
     1081 ctgtaagtat tccagttata ataagatttg taattcctaa attaattcca ttacgtaatg
     1141 caaatatgga gtatacagct ggaaataata ataccagggg agtataggct atacctaaaa
     1201 ctattagaaa ggttattccc gtaattaata taagtgattc tttaattttt ctatttatct
     1261 gattgttgcg caaataagtt cacctctagt ctatctggaa tctatatggt ttaacagttt
     1321 acttaggtca ccataccatt tttcaatctt atgttcttga attaaattta ttttaatatt
     1381 ttccttcaac gcttgatcta tattttcata ttctaaccct aatcttttcc ctaaaatata
     1441 tgtttctagt attatattgg aaataatatc ctccagatct tctcttgtat tatcttcatt
     1501 attaacaaga accttatgta aatatgctat attagtcaat aattctgcct ttagccattc
     1561 tacagtcttc atattcttaa ttatatctat gtttttatta acctccatat tacccatcct
     1621 tttttctatt tctttcaatg tccttatcta gataatacag agttaaggat aataagtcaa
     1681 atgaaaaaaa gagaggttat ttcctctctt agtctgcact gtaaggtagt aaagctacct
     1741 gtctggctct tttgatggca gtagttaatt ccctttgatg tttagaacaa ttacctgaaa
     1801 ttcttctagg aagaattttt cctctatcag taacatattt ttttaattta tttatatctt
     1861 tatagtctat tctttctgat ttttccgcac agaaactaca gactcttttt cttggtctaa
     1921 atcttctttg cattattttc ccctccttct agaacggaat atcatcgtta tctactggat
     1981 gcaaaccatc tatacctggg aaatctgaat cttgggcttc aaagcctgca tcattacttc
     2041 taggatctcc ccattctaga aattgtacgt tatttgctac tacatctgta gtatatacag
     2101 ttttaccgtc cttatcctca tagctgcctg tttggattct gccttgaaca gccacaagcc
     2161 tacctttggc taagtaattg gcacaatttt cagcctgttt cccccaaacc acaatcctaa
     2221 taaagtcagc tgtcggttgg tttcttgctt ccagttcctg ttttttttct ctagacagtt
     2281 gcttatcgac tgccaatgta aacctagata ctgcatttcc agatgggata tatcttaaat
     2341 ctggatctgc aactagccta cctattaata caacattatt catttacttc accatccttt
     2401 gactattagt cttctctgat gatcatgtgt ctcataatgc tgtcagaaat ctttgcaatc
     2461 ctatctaatt cgttaactgt ctctggagtt ccctggaaat tgactattac gtagtagcct
     2521 tctcctatat cattgattaa ataggcaagt tttctaactc cccactcttc tacatttgta
     2581 acttctccat cttccttgat aatggattta aacctttcaa gcaattggtt tctactttct
     2641 tcttcctcat ttggtaagaa gataaaaaca gcttcatact ttctcatttg cttcacctcc
     2701 ttatggacta tcggccctat ttagtatagg gcaaggaata ccctagtatt atatcattat
     2761 tgaataagct ttacaagctt aatgtaaata cattacaact atatactgca gatctatttt
     2821 tcagcaagtt ggtaaatttt aattacatcc tcttttccca gtttttttaa acctgcctaa
     2881 aggaccattt tccgttacct tttctgccat caaatggatc ttatctgtag gcaagcctgc
     2941 ctctttaaag ctggtaggta gacctagctc tttaaggaag ccttcgaatc tatatatacc
     3001 ttctaaagca atatcctccc tagtttttcc atctgttgat acctgaaaaa ctcgtttggc
     3061 aaactgaacg aatctatcta aatttgtctt atatacatat ttcatccaga caggaaaaac
     3121 tatggatagg gttataccat gggtttcatc atatagggcc cctatttcat gaactataaa
     3181 atggctggcc caatcctctt ctcttcctag tcccaacagg ccactgtggg caaggctccc
     3241 agccagcata atctgggccc tagtattata atcttgtggg tcttttttta aaatgtaaga
     3301 gaatattttt atactttcct cctattagtt tccctaactc ataaactgca tcttgtccaa
     3361 ataccaactg ggtctggcac tgatagctga agctttccaa agatatccct ctcttcatat
     3421 atacttgttc taactttctt ctggcttatg gtggacaatg gaaatccact tttcgtctac
     3481 caagatcttt ctaactctct tttcaaaatt tatcctggta agccaaatct gcctatcaca
     3541 ccctaagcac tttagcttta tatctacccc tgtccttaat atttcccact tgttctctcc
     3601 acaggggtgt cccttcttaa gggttataat gtctccaact ttatagtgta aaatcattct
     3661 ttctctcctc caataataat tctttttgga taaggaatct ctattccttc tttgtctaag
     3721 gtttccttta ccattttcct tattgccctt tcaacagccc actgttccat aggctttgtt
     3781 ctggctatta tattaattac aaatccatat tcagccaatt cagcaactcc caaggccacc
     3841 ggaccttcta ctatggcctt attttccttt ttaagccttt cgcaggcctt atttaggacc
     3901 tccatagccc tatcaatgtt ttcttcatag gctatagtta cctgaaccag ggctctcatg
     3961 gcccctctag ttttgtttgt aaccgtctgt atgctgctgt taggtataat gtggagttct
     4021 ccggagaagg cccttaattt agtaacccta agtcctaatt cttctaccac tccttcaaag
     4081 tctcctgttt caatcagatc acctactgaa tattggtcct ctaatagtat aaaaaaaccg
     4141 gtaattatat ccttaactaa ggactgagca ccaaatccta tggccaggcc tcctatcccg
     4201 gctgtggcca aaatagaagt ggtaggaaca ttaaacatat ctagcaccat cattatgcct
     4261 ataaatgtta agatatagta taccagcttt tttaggacca gtgccaatgt attagccctt
     4321 ttgtcgtcta ttgtagtgct tttagtcttt ctggtagata atgttttatc aatcaccttg
     4381 tttgctatcc tgttaactaa ccaaataact acaaatatta atattatctt tattaatctt
     4441 ccaagtatat ttaaattacc tatattatct ttcagatagg tctctactaa ataattaata
     4501 ttttccatat ttccccctaa actttatatc agcctttcaa ataaattttt atggtttacc
     4561 ttggtatagc caaataatcc atctattgtt atttgaccct tatctattat agcctgaacc
     4621 ctggccagat catccatcat aaacagcaat gataggccac agctatggct aacctccctt
     4681 ggtgtcggta tagtcttaca ggttatatgt tcatttttga atagtctttc tgcctccaag
     4741 gcaaaatggg tagttttaaa agttattaga ccatattctt ctcttttcat atttaactcc
     4801 tatcatatta ttatattttt ttcactttcc cttaatcttt catatatggt atacatatta
     4861 gaaatagaac ctacttctaa tttatcctta atatttaaaa agtccaggca ggttccacag
     4921 gagataattt ccactcctgc atcctctaac ttctttaaat catctaaaac ctctgatcct
     4981 tctgtagtta gtctaactcc cccattataa aatactaggg ttgagggata aggtttagtt
     5041 tcagaaagag tatagatgaa acttttgatt agaatttctc ctagcctttc atcgccttta
     5101 cccatagtgt ttgaggatat accaatggtt atatcttcaa gctcataacc tatatttgtc
     5161 tctaccccag cttctctatc tcctttttct atacttatat aataatcatt cccctttttt
     5221 tcgacaatac tacttagtcc cattccctgg gccagtttag atacattttc ctttgcaacc
     5281 tcattatcta caattgtaac aaccagtcct tggttaatct catccaattt ctgcttagtc
     5341 ataataactg gttttggaca ggcctggccc ctggcatcta tttctatcat tttatcaagc
     5401 tcctttgttc aaaaatatat actaacacat taattgtata ataaaattaa tttattttcc
     5461 acaggaatgg gataatattg gattttaaga taaatttcaa gttaccttaa aaatattaat
     5521 caatttatta actttattga tatatgtatt gacattttta aggtatggct ttattatata
     5581 attaagaaca gctttaaaac aatggatgca ttataaaaat tattacattc cagttaaggg
     5641 gaagtgagaa aaatgaatgc taaagtaatt ggaaaatgtc cagtctgtaa ccataatctt
     5701 aaagtaacta gattaagttg taacaattgt catactagca ttgaaggaaa ttttgactta
     5761 tgtaagttta gtagattgtc caaggagcaa agggcctttc tagaagtttt tgtaaaaaac
     5821 aggggaaata taagggatat tgaaagagag ttaaatattt cttatccaac agttaaaagt
     5881 cgattggata atttaataga aagcctgagc tacaatccta aggacagtga acctgctatt
     5941 agcagaagag atgtccttaa aaggttagaa aatggccaaa taactcctaa agaagctgtc
     6001 aagcttctta aaggtgaata atttataaat ggggtgagga aatgcctgaa aaattacaaa
     6061 tattaaatat ggtaagtgaa gggaaaatat ctgctgagga aggaatggag ttgttatcag
     6121 ctctggatga accaaaacag gatggcaggg atagacagaa gagaagatcc ttaagggtta
     6181 agatccatgg ccctagtgag gatgctaagg taaatgcaac tataccaata tcacttatta
     6241 ggctaggatt taaacttgca tctaagcttt ctcctgagtt tagagaggct ggtctagctg
     6301 agattgatct tgaagagatc attgaagcaa ttgatgatgg ggcagttgga actattgttg
     6361 aagtagatgc tgatgacgga accaagattg aggtaatagc tgaatagttt taaaaatgga
     6421 tactaaaatt ttagtatcca tttttattta agaggcttat ttatggtata ataaatgtac
     6481 ccatacaata aggaggatcc atatgcttaa attatctgta gataaagatt caactacacc
     6541 cttatatgtt cagatctatg attctataaa acaattaata gaggagggca aaattaaggg
     6601 ggataaactt ccttccatca gatccttggc caaatcctta aatgtaaata atgtgaccat
     6661 tgtcaatgcc tacaagttac ttgaacaaaa tgattatgta tactctatta aaggtagtgg
     6721 aacctatatt aaaagaaccc tcaatcctga agaattctct tatcttgaag agggtgatat
     6781 ggagctgatg gtatcaggag tcctgcccat atctgataat agcattaatt ttgcatctgt
     6841 atctcctaca cctgatttat ttcctattaa tgaatttaaa caagccctag tccaggtttt
     6901 agatagggac ggtggccttg cctttttata tcctgaagtt actggatatg aacctttaag
     6961 ggaatctata tctaaatttc tatttattaa ttataagaca agggttaata aagataggat
     7021 acttattact tccggtggcc agcagggttt agatataatt tcaaaaacat taattaacca
     7081 gggagaccta attttagtgg aaaacccaac ctatttgggg gctttggcag cctttgaatc
     7141 caggggggct agagttgtag gcatacctat gatggaagat gggattgatg tagatttatt
     7201 agaaagctat gttaaaaagc ataatccaaa attcctctat atgatgacta actatcaaag
     7261 ccctaccact tattcctata gtcaagaaaa aaagataaag gttattgaac tggccaatag
     7321 gtataatttt tatataatag aggatgattt cttaacagac ctatccttta actgcgagat
     7381 gaaaactcct ctcaaatcca tagacaaaaa tgataaggtt atttttataa agtccttttc
     7441 aaagattttt atgccaggga ttaggatagc ctttgttact gtgccaaaca ttttatttag
     7501 ggatgttatt aaggctaagc ataccaccga tgtttcttcc tctggatttt tacaaagggc
     7561 ctttgaccaa tatttaagga caggttattg gaaacaacat attaataagg taaagaaagt
     7621 ttacaaggaa agatatgaat taataactag ggaaatagat agattaaagg tatatggcat
     7681 aaattatctt cctcccaagg gtggtcttag cctgtggctt agcctgccaa aacatattga
     7741 tgccattcag ctataccagc agtgtaatga agaaaattta gccctggtac ctggaaaggt
     7801 cttttttatt aacgatgatt taaacacaaa ccatattaga ttaagctttg gcaatgtgag
     7861 taatgaagaa attattaagg gcataagcat attggaggat ataattagaa aacaatcttc
     7921 tagtcacacc actagcaaat atttaccctt tgtataagat tatgggatgg ttagttattt
     7981 attagagata attattcgga aaatcattat agagcctatt agaccgaaaa cagggtatat
     8041 aataccaatt aattttgcaa atcctaacct ggccaaaggg attgtggata tagaaaatat
     8101 aactattaca attattctat tcctacttct tcccaaccta tttacaaagc cgaatccatt
     8161 ggccaaggca gttgtaaaca tggctatcca aaggattaga gaatatattt tcccataatc
     8221 cttgctaata taattactaa tttttaacat aggtacatct acatattgga tctctataaa
     8281 atgtaaaagt atagagtata atatggccat acctaaaata taaaggataa atcctcctgt
     8341 taatccacct aaaatcgccg tccgcctact atctattaga tcacctaaag aggaaaatac
     8401 aactattatc aataaagaat ttgatccaaa atacagaata gccgaactta taaaattgcc
     8461 ctttagggta aagcttatcc catctaaata gggaaattta taccctgtct tgctactaaa
     8521 ataatgacta gtaaataaaa tacctattat aagtagggga accagtatag tgttaattaa
     8581 agataggccc tctaaattaa ataaaaatac aataaaacaa cagaccagca taactacgac
     8641 tcccatataa aagggcagtc ctagttgttc tttgaaaact gctccacttc ctgccaccat
     8701 cactgaaaac cctgtataaa gggatagtat tgttaaggca tctaccagtg gggcaagctt
     8761 ttttccaaag acatcttgat ttaattgatc aaaattttta acctgccctt tatgtatttt
     8821 atataatagg aggctaccta cagctgcaaa taaaagtcca gctatataaa tgccccatat
     8881 acctttagct ccgtagatag caaaaaagtc taatatctct ctgcctgaag caaaacctgc
     8941 tcctattact gttcctatat atattgatgc tatttttatc caatttctat tcataaacac
     9001 cctcctaaca ataaatcata ttattagtag ggcttattct ataactgaaa actaaattta
     9061 aagcctgtct gtttaaaaat ctaatactcc aatataaaaa caccccattc taaatagaat
     9121 ggggttaatt aatctggcag cttcctactc tcccaggacg tctccgtcca agtaccatcg
     9181 gcgttaagag gcttaacttc tgtgttcgag atgggaacag gtgtgaccct cttgctatcg
     9241 ccaccagata aactaaaaaa gcaatatat
//
LOCUS       DATGSI010000118         1069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_432056, whole genome shotgun sequence.
ACCESSION   DATGSI010000118 DATGSI010000000
VERSION     DATGSI010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432056"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..162)
                     /locus_tag="VK031_01815"
     tRNA            complement(92..162)
                     /locus_tag="VK031_01815"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(128..130),aa:Pro,seq:tgg)
     gene            complement(347..430)
                     /locus_tag="VK031_01820"
     tRNA            complement(347..430)
                     /locus_tag="VK031_01820"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(394..396),aa:Tyr,seq:gta)
     gene            complement(443..513)
                     /locus_tag="VK031_01825"
     tRNA            complement(443..513)
                     /locus_tag="VK031_01825"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(479..481),aa:Arg,seq:tct)
     gene            complement(526..596)
                     /locus_tag="VK031_01830"
     tRNA            complement(526..596)
                     /locus_tag="VK031_01830"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(562..564),aa:Trp,seq:cca)
     gene            complement(608..679)
                     /locus_tag="VK031_01835"
     tRNA            complement(608..679)
                     /locus_tag="VK031_01835"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(644..646),aa:Glu,seq:ttc)
BASE COUNT          383 a          236 c          152 g          298 t
ORIGIN      
        1 ataacacacc ctcggatccc cccactgccg ccccacctaa cttaacatgc ccaacgagcc
       61 ctatataccc aaaggaatag gtatatagga ctcgggatgg ctggaatcga accaacgcca
      121 attaatccca aattaacgat actaccacta tacgacatcc cgccgctttt aaaataatcg
      181 gggaaggatt tatcaaagta aggctagaac cggtctcgaa ccgatattaa ataatttgca
      241 atcattcaca ttacctatta tgttatctag ccttttctta tcctatcctg ccttactcta
      301 atctttagcc cctgcccgat tattttaaaa taaaaatata ataaattgag aagagcagga
      361 ttcgaaccta cgaaggaaaa gctccactaa atttacagtc tagcaccttt ggccactcag
      421 tcatcttccc aaactaaatt aaaggatgca ttaggaatcg aacccaaatc aatagattag
      481 aagtctacag ctttcccatt aagctatgca tcctttaatt tagttcagga atggaaggag
      541 tcgaaccctc aacgctggtt ttggaaacca atattatacc atttaactac actcctggga
      601 tgtaatatat cccctggggg aattgaaccc ccattttcag agtgaaaatc tgaggtccta
      661 accattagac gaaggggaca cacccctccc cgcaagtata taaatctatg ataccatttt
      721 tttataaatt gtatgtttca ttaataaaat ccaaatttcc cataaaggtt aacaaagtaa
      781 cttcagaata acagaattaa aatatcccca agagcctacg tagttgtcct taaaatcctg
      841 aaatttaatt attaaatgaa ctcttaaaaa aatgaaaaat tccgaattat ccttaaatta
      901 ttacttcaat ttttcaaaaa taaccggata taaactatct aaatttaaac caaaggggta
      961 aggtatcctg gaaccaaaac ctacgcgcga attacaattt acccaaataa aaaatttatt
     1021 ataatttatc aaagcatgag ctggacgaat aaacgcaaat ggtttcttt
//
LOCUS       DATGSI010000119         1345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_528760, whole genome shotgun sequence.
ACCESSION   DATGSI010000119 DATGSI010000000
VERSION     DATGSI010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528760"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VK031_01840"
     CDS             <1..358
                     /locus_tag="VK031_01840"
                     /inference="COORDINATES: protein motif:HMM:NF015728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HLS52684.1"
                     /translation="AVDKKKIDPSEVKQALTHVRNFTSIEADGSHKNGSLRQMRVIKK
                     GLNFEVEINYERDLKDKELGLLAASLLQLRHIGTMRTRGKGEVRCSLHVLENGSYRDI
                     TADYFNLFLKEVNYNE"
     gene            351..>1345
                     /locus_tag="VK031_01845"
     CDS             351..>1345
                     /locus_tag="VK031_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52685.1"
                     /translation="MSKYLVFNIESLENLKFSSTNVQSDSEDTNEYIPGTSIRGAYIY
                     RYMIKKDIKDINQGEHRDKLLNGKIKFLNAYPIYANKRGMPLPKVYFARKDELKSEQN
                     KIDMYPGLGQDLGAGYGKVRACEFVGWEKDSFYRIKVDKISNLHINKMNNKEPNKLFM
                     YEAIKAGQSFQGIIKVEDDEYIEEVREIFENTIVYLGGSKGSGYGKCQIGNISIMEEN
                     PELEYFDQSLKGQEIYLLALSDIIYRNEYGEYKTYIEEKDLEKELGLEKVEFIDSSIE
                     TKAITNFNNKWNCSTPIINGIKAGSVLKYRITEELNLDSIKKFMDKGIGERRIDG"
BASE COUNT          541 a          147 c          291 g          366 t
ORIGIN      
        1 tgctgtggat aagaagaaga tagatccttc tgaggtgaag caagcgttaa ctcatgtaag
       61 gaattttact agcattgaag ctgatggatc gcataaaaat ggttcgttaa gacaaatgag
      121 agtaatcaaa aagggattaa attttgaggt agaaataaat tatgaacggg atctaaagga
      181 caaagagtta ggtttattag ccgcatcctt gcttcaacta cgacatattg gaaccatgag
      241 aaccaggggc aaaggagaag tgaggtgttc cttacatgtg ctggaaaatg gttcttacag
      301 ggatattaca gcagactatt tcaatttatt tctaaaagag gtgaattata atgagtaagt
      361 atctagtatt taatatagag tctttggaga atctaaaatt ttcgagtaca aatgtacaat
      421 cagactctga ggatactaat gaatatatac caggcacttc cataagagga gcctacatat
      481 atagatatat gataaaaaaa gacataaaag atataaacca aggtgaacat agagataaac
      541 tgctaaatgg aaagataaag tttctaaatg catatccaat atatgcaaat aagcgtggta
      601 tgcctttgcc taaggtctat tttgctagga aagatgaact taaatctgaa caaaataaaa
      661 tagatatgta tccaggatta ggtcaagact taggtgcagg gtatggaaag gtaagagcct
      721 gtgaatttgt tggctgggaa aaggattctt tttatcggat aaaagtggac aagatatcaa
      781 atctgcatat aaataagatg aataacaaag agcctaataa attatttatg tatgaggcta
      841 tcaaagccgg tcaaagtttt caagggatta taaaagtaga agatgatgag tatatagaag
      901 aagtaaggga aatatttgaa aatactatag tatacttagg aggatctaaa ggtagcggtt
      961 atggaaaatg tcaaattggg aacataagta ttatggaaga aaatccagaa cttgaatact
     1021 tcgaccaaag tttaaaggga caggagatat atttgttagc tctatcagat attatttaca
     1081 ggaatgaata tggagagtat aaaacttata tagaggagaa agatttagaa aaagaactgg
     1141 gattagaaaa agtagaattc atagatagtt caatagagac caaggcaata actaatttca
     1201 ataacaaatg gaattgctca actcctatta taaatggaat taaagcaggt tcagttttaa
     1261 agtatagaat aacagaagaa ttaaatttgg acagtataaa aaaatttatg gacaagggaa
     1321 taggggaacg aaggatagat ggatt
//
LOCUS       DATGSI010000120         1111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_243118, whole genome shotgun sequence.
ACCESSION   DATGSI010000120 DATGSI010000000
VERSION     DATGSI010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243118"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1111
                     /locus_tag="VK031_01850"
     CDS             <1..>1111
                     /locus_tag="VK031_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52686.1"
                     /translation="VGHNFYGFDLIALKKIFPDINIRATIIDSLLISYYIEPNRMVHG
                     LEKYGEDFGVPKPEVTEEQWKGIGMTKEEHYELMKHRVSEDVKINTKLWLHQLRKYRS
                     IYDGDDDKIKNHINFLNVKGKMYHDMYLNPLKIDVKKAEEHRDILQNKFDSLTSELSK
                     VMPKIPSYVVRKKPKNLYRKDGTLSVAGERWKKVTSEHGVDLEYEGDLKELMSLEEPN
                     PNSTQQVKDWLFHLGWKPELFKETVNKAGEKNKVPQLKDMDKNLCKTILKLEEKEPKV
                     RLLEDLSVLGHRLSLVKGFLRDCDENGNIVAKMGGLANSLRIKHRTIVNLPSVSSQFG
                     EYIRSLIIPQEGFKFIGSDISGLENQTKLNFIVDID"
BASE COUNT          429 a          143 c          236 g          303 t
ORIGIN      
        1 tgtaggacac aatttttacg gatttgacct tattgcttta aagaagatat tcccagatat
       61 aaacataaga gctacaataa tagacagtct tcttatttct tactatattg agccaaacag
      121 aatggttcac ggattagaga aatacggaga agattttgga gtacctaagc cagaagttac
      181 agaagaacaa tggaaaggta taggaatgac taaggaagag cattatgaac ttatgaagca
      241 tagggtttca gaggacgtaa agataaacac aaagttgtgg ttacatcaac taagaaagta
      301 caggtctata tacgatgggg atgatgataa gattaagaac cacataaatt tccttaacgt
      361 aaaaggaaag atgtaccacg atatgtacct aaatcctctt aaaatagatg taaagaaggc
      421 agaagaacac agagacattt tacaaaataa gtttgacagc ttaacctctg aattgtctaa
      481 ggtaatgcca aagattccgt catacgtagt tcgaaagaaa cctaaaaatc tttacaggaa
      541 agatggtact ttgtctgtgg caggggaaag gtggaagaaa gttacatcag aacatggagt
      601 tgatttggaa tacgaagggg atttaaaaga gcttatgagt ttagaagaac ctaatccaaa
      661 cagtactcaa caagttaaag attggctatt ccatcttgga tggaaacccg agttatttaa
      721 agaaactgtg aataaagcag gagagaagaa taaagtacca caattaaaag atatggacaa
      781 aaatctttgt aaaactattt taaagttaga ggagaaagag ccaaaagtca ggttattaga
      841 ggatttgtct gttttaggtc acagactaag tcttgtaaaa ggatttttaa gagattgtga
      901 tgaaaatggg aatatagttg caaaaatggg gggtcttgca aattctctta ggataaaaca
      961 tagaactatt gtgaacctcc cttccgtgtc ttcacaattt ggagagtata taaggagttt
     1021 aataattcct caagaagggt ttaagtttat aggttcagat atttcagggt tagaaaatca
     1081 gacaaagtta aattttatag tagatataga c
//
LOCUS       DATGSI010000121         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_432309, whole genome shotgun sequence.
ACCESSION   DATGSI010000121 DATGSI010000000
VERSION     DATGSI010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432309"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VK031_01855"
     CDS             complement(<1..729)
                     /locus_tag="VK031_01855"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HLS52687.1"
                     /translation="MAAWFNILIPSLLPFLFFSEILILLGFVDILGELLEPVIRPFFN
                     ISGQGAFPFLMSIISGYPVGAKLTSSLRKKNMISLVEANRLIAFASTSGPLFILGAVA
                     IGMLNNPNLASLMLMPHYLAALSLGFIFSFYQSNESTRFHRKKKKDYGFSHLFQAFKY
                     SQDRPLGVIISKSIQDSTTSIILIGGYVIIYSVIIDLILSSSMTISILSTLSNYFNLS
                     YDLLEALVAGLIEISTACFKVSSLN"
     gene            641..823
                     /locus_tag="VK031_01860"
     CDS             641..823
                     /locus_tag="VK031_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52688.1"
                     /translation="MSTNPNKINISEKNKKGSKEGINILNQAANPLLALLIVRVGFFI
                     IFIIKKDKASKINKFE"
     gene            complement(914..2164)
                     /locus_tag="VK031_01865"
     CDS             complement(914..2164)
                     /locus_tag="VK031_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HLS52689.1"
                     /translation="MKTVALITEYNPFHYGHLYHLKESIKKTQATHSIAIMSSSFMQR
                     GEPALIDKWTRTKMAINNGVDLVIELPFVYACQSAELFASGAIKILDALKVVNFLSFG
                     SEEGRLKNLNTLADILVKEPLAYKNRLRYYLNQGLSFPVSRSKAIEDYLKTKTNDISI
                     YRQVLKRSNNILAIEYLKVLKRIDSSIKPVTVKRIGASYKEEKLHSKFASATGIRNLL
                     LSSNFKEVKNYLPKASYELLGEYRSQYGNYNSLENYRDIIKYILTLDPKEKLKNILDN
                     EPGLENRIVEIGLLEHKIGDIVKNISTKRHPETRVQRILIHLLNELYEPDVKELLEYE
                     PQYIRVLGFNNKGIELLREIKAKTNLDIITKFANYPRLNNPIINKFLSYEERATNLYF
                     LGVNFDSPLRDMDYLTSPYINKKI"
     gene            2315..>2897
                     /locus_tag="VK031_01870"
     CDS             2315..>2897
                     /locus_tag="VK031_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HLS52690.1"
                     /translation="MNILVINCGSSSLKYQLIDMDGEHVLGKGLVERIGIEGSIIKHK
                     TTGKDEVEIKEDMKDHKRALELVLDVIVGEENGAIGSMDEISAVGHRVVHGGEEFSES
                     AIINDQVMETIEECIALAPLHNPPNIMGIEACKELMPNVPMVAVFDTAFHQTIPMENY
                     IYPLPYEFYEKHQIRRYGFHGTSHKYVSLRAAEI"
BASE COUNT          956 a          452 c          515 g          974 t
ORIGIN      
        1 atttaaggat gaaactttaa aacaggcagt actaatctct attaggccag ccacaagagc
       61 ctccagtagg tcatagctta gattaaagta attggataaa gttgatagaa tagagatggt
      121 catactagaa cttaggatta gatcaataat aactgagtat ataataacat aaccacctat
      181 taggataatt gaagtagtgg aatcctgaat acttttagat atgataacac ctagtggtct
      241 atcttggcta tatttaaagg cttgaaaaag atgagaaaac ccatagtctt tctttttttt
      301 cctgtggaat ctagtagatt cattgctctg gtaaaaacta aatatgaagc ctaggctaag
      361 ggctgccaga taatgaggca taagcatcaa agaagctaga ttagggttat ttaacatgcc
      421 tatggcaacg gcaccaagaa taaagagtgg gccagaggtg gaggcaaaag ctattagcct
      481 gttggcttct actagactta tcatgttctt tttacgtaaa ctagatgtta atttggcgcc
      541 aacaggatag ccagaaatta tactcattag aaaaggaaag gccccttggc cagatatatt
      601 aaaaaagggt cgaataacag gctccaatag ctcgcccaaa atgtctacaa atcctaacaa
      661 aatcaatatt tccgaaaaga acaaaaaagg cagtaaagaa ggtattaata tattaaacca
      721 ggcagccaac cctcttttag cactattaat tgttagggta ggcttcttta taatatttat
      781 tataaagaag gataaggcta gcaaaataaa caaatttgaa taaaagtttt tcccttttcc
      841 ttgtctcata aaaatcactc cctataaatt atatttttaa aaaatatatt tatgagggag
      901 tgatttaatt acttcaaatt tttttgttta tatagggact agtaagatag tccatatccc
      961 ttaaaggaga atcaaagtta actcctaaaa aatataaatt ggtggccctt tcctcgtaag
     1021 ataaaaattt gtttataata ggattgttaa gcctaggata atttgcaaat tttgttatta
     1081 tgtctagatt ggtttttgcc tttatttccc taagtaattc aatgccttta ttattaaagc
     1141 ctaagaccct tatatattga ggctcatatt ccaataattc cttaacatca ggttcataca
     1201 attcatttaa taagtgaatc aagattcttt gaaccctggt ttctgggtga cgtttagtac
     1261 ttatattttt tacaatatcg cctatcttat gctctaaaag ccctatttca actattctgt
     1321 tttctaaacc aggttcatta tctagtatat tttttaattt ttccttagga tctaaggtga
     1381 gtatatattt tataatgtcc ctatagtttt ccaatgagtt ataattccca tactgggatc
     1441 tatattcacc taaaagctca tagctggcct tgggaaggta attttttacc tccttaaagt
     1501 tactggataa aagtaggttt ctaatacctg ttgcagaagc aaatttacta tgaagcttct
     1561 cctccttata actggcgcca attcttttta cagtaactgg ctttatggag gaatcaattc
     1621 tttttaagac cttaagatat tcaatggcta aaatattgtt agatcttttt aatacttgtc
     1681 tgtagatgga aatatcattt gtctttgttt ttaaataatc ttctatggcc ttgcttctgg
     1741 aaactggaaa gctcaggccc tggttgagat agtatcgtag cctattttta taggctaggg
     1801 gctcttttac taatatatct gccaaagtat ttaaattttt caatctacct tcttcgctac
     1861 caaaggataa aaagtttact acctttaaag catctaatat ttttattgca cctgaagcaa
     1921 acaattcagc actttgacag gcatagacaa agggcagttc tattactaaa tctaccccat
     1981 tattgatggc cattttagtt cgggtccact tatctattag ggctggctca cccctttgca
     2041 taaatgaact gctcataata gctattgaat gggtggcctg agtcttttta atagattctt
     2101 ttaagtgata taggtgtccg taatgaaatg gattatattc agtaattagg gctactgttt
     2161 tcatatctaa ctccttttta atgatagttt cattataaca tatcttttgc ttttaggact
     2221 acttgctaaa tttaattata agaagattgt gttgattttt taacgataat ataatactat
     2281 tgttatagtg aaagaagata taggaggaat taagatgaat attttagtaa ttaactgtgg
     2341 cagttcttca ttaaaatatc agcttataga tatggatggg gaacatgttt taggaaaagg
     2401 tttagtagaa aggataggta ttgaaggctc aataataaag cataagacta ctggaaaaga
     2461 tgaagtagaa attaaagagg atatgaaaga tcataaaaga gccttagaat tggtacttga
     2521 tgttattgta ggtgaggaaa acggagctat cggctctatg gatgagatca gtgctgtagg
     2581 ccatagagtg gttcatggtg gcgaggaatt ttctgaatct gctattataa atgatcaggt
     2641 tatggaaaca atagaagagt gtatagcttt agccccacta cataatccac caaatattat
     2701 ggggattgag gcttgtaagg aattaatgcc aaatgtacct atggtggcag tctttgatac
     2761 agccttccat cagactatac caatggaaaa ttatatttat ccgttgccct atgaatttta
     2821 cgaaaagcat caaatcagac gctacggctt ccacggtaca tctcacaaat atgtatcctt
     2881 aagagctgca gaaattt
//
LOCUS       DATGSI010000122         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_338108, whole genome shotgun sequence.
ACCESSION   DATGSI010000122 DATGSI010000000
VERSION     DATGSI010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338108"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VK031_01875"
     CDS             complement(<1..350)
                     /locus_tag="VK031_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLS52691.1"
                     /translation="MKEKVLETIRNYNLIRPKENILVGVSGGPDSMVLLYLLMELKDD
                     LDFNILVAHVNHGVRGKEARRDQEFVKEQAKKLGLPYFSKKVDMIAYAKAKGISEEEA
                     GRKLRYGFFREVLA"
     gene            complement(445..>2155)
                     /locus_tag="VK031_01880"
     CDS             complement(445..>2155)
                     /locus_tag="VK031_01880"
                     /inference="COORDINATES: protein motif:HMM:NF039380.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HLS52692.1"
                     /translation="YTEGALMGTGLGLTIGMVSYMSHPEMPFIIALLTAGGLLAGLFR
                     ELGKAGSALGFVLGNGIISFYINRFGISFFAYREILIAIPAFFLLSKYLKLDLKAYLA
                     GNQQVKDEYKDKRDRLTITRLNNTGTLFNNLSQILEKSIEKERNYSTSQVYNLIDKIS
                     NESCKSCIKYQSCWKEDYYITYYNIFNLIGNVESKLKGKRLQTFKRKCINYNALKENT
                     LKIYEDYKRDHSWNMKLLEQRQLLAEQVENLGQVVNQLAREIDKRPIFNEQLENLLIK
                     ELKNKKIHVVELLVVELADGMEIFVEVDTSQNPNSPYLSKLEELISNALGFPVVAATS
                     YGNILDDIKSYRFIRANRYSTLTKVLSQSNSENLISGDSYTYGQYDNVSFMAISDGMG
                     IGKKANEESQIAIDLLEKLMETRMDKSIIIKTINSILKGRTDNEIFTTLDLAFIDLYT
                     GKLQSIKNGSPTTFIKRADGVSLINNRSLPLGILENVDLDVYEEELEDGDIVIMMSDG
                     ILESNRNVKDSESWMKDIIASIDSWNPQAIAKEIMEIAEFVSGNGIRDDMTIMATKIW
                     RNL"
BASE COUNT          647 a          456 c          288 g          764 t
ORIGIN      
        1 ttagctaaaa cttctctaaa aaagccatac cttaactttc tacctgcctc ttcctcgctg
       61 atgcctttgg ctttagcata ggctatcata tctacctttt ttgaaaaata gggtaagccc
      121 agcttctttg cctgctcttt aacaaattcc tgatcccttc ttgcttcttt acccctaacc
      181 ccatgattta catgggccac taatatatta aaatccaaat catctttaag ctccattaac
      241 aaatatagta ggaccataga atcaggtcct ccagataccc ctactaaaat attttccttg
      301 ggccttatta aattgtagtt cctaatagtt tccaaaactt tttccttcac taaagatcac
      361 cactatactt tctatatata atatacccta ttacttgata aaaacaaagg ggtaactata
      421 gttaaaaagg agaaatctat tctcctatag atttctccaa atttttgtag ccataatagt
      481 catatcgtcc ctgataccat tgccacttac aaattctgct atctccatta tttctttggc
      541 tatggcttgg ggattccaag aatcaatact cgcaattata tctttcatcc aggattctga
      601 atctttcaca ttcctattgc tctctaaaat cccatctgac atcataatta ctatatcccc
      661 atcttccaac tcctcttcat atacatctaa gtctacattt tctaaaatac ctaagggcaa
      721 ggacctatta tttattaggc tgactccatc cgcccttttt ataaaggttg ttggagatcc
      781 atttttgata ctctgtaatt ttccagtata tagatcaatg aaggccaaat ctaaggtagt
      841 aaaaatttca ttatctgttc tccccttcaa tatggaattt atggttttta taataataga
      901 tttatccatc cttgtttcca ttaacttctc caatagatca atggcaattt ggctttcctc
      961 attggccttt tttccaatac ccatgccatc acttatggcc ataaaggata cattatcata
     1021 ctggccatag gtgtaactat ctcctgatat taagttttca gaattagatt ggcttaaaac
     1081 cttagtcaag gttgaatacc tattggctct aataaatcta taacttttaa tatcatccag
     1141 tatattgcca taggatgtgg ctgccacaac tggaaaacct aaagcattag aaattagctc
     1201 ctcaagctta cttaggtaag gactgttagg attttgagat gtatccactt ctacaaaaat
     1261 ttccattcca tcagctaact ctacaaccag caactctact acatgaattt ttttattctt
     1321 taattcctta attaataaat tctcaagctg ttcgttgaaa ataggtcttt tatctatttc
     1381 cctggctaac tggttaacaa cttgtcccaa gttttccacc tgctctgcta aaagttgtct
     1441 ttgttccaat aatttcatat tccatgagtg atccctttta tagtcttcat atatcttgag
     1501 agtattttcc tttaatgcat tataatttat acactttctt ttaaatgttt gaagcctctt
     1561 tcccttaagc ttggattcca cgttacctat tagattgaaa atattatagt aggtaatata
     1621 ataatcctcc ttccaacagg attggtactt tatacaggat ttacagcttt cattagagat
     1681 tttatctatt aaattatata cctgggaggt tgaataattt ctttcctttt caatagattt
     1741 ttctaggatt tgacttaaat tattaaaaag ggtgccagtg ttatttagcc tggtaatagt
     1801 taatctatcc cttttatctt tatattcatc tttcacctgt tggttgcctg ctaaataagc
     1861 ttttaaatcc agtttaaggt atttagatag gaggaaaaag gcaggaatag ctattaaaat
     1921 ctccctatag gcaaaaaagc ttattccaaa tcgattaata taaaagctaa taatcccatt
     1981 gcctaagaca aaccctaagg cagaaccagc cttgcctagc tccctaaaca agccagccag
     2041 taatcctcca gctgtcaata gggcaattat aaaaggcatt tcaggatggg acatatagga
     2101 taccatacct atggtaaggc ctaggccagt tcccataagg gccccttctg tatag
//
LOCUS       DATGSI010000123         1039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_104371, whole genome shotgun sequence.
ACCESSION   DATGSI010000123 DATGSI010000000
VERSION     DATGSI010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104371"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VK031_01885"
     CDS             <1..322
                     /locus_tag="VK031_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HLS52693.1"
                     /translation="NQKIHTIWGQAPEDLPDKEFNKCFIGGSRGQLAGIFSYLSSNLL
                     EGGIVCANFITLDNLNEFNKLLLKYNYKDIEINLIQASQVNRLGMLKGQNPIFIIKGV
                     KSNA"
     gene            315..>1039
                     /gene="cobM"
                     /locus_tag="VK031_01890"
     CDS             315..>1039
                     /gene="cobM"
                     /locus_tag="VK031_01890"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HLS52694.1"
                     /translation="MLSFVGAGPGHVDLITVKGRQLLEEADIVIYAGSLVSNEHLKFC
                     KKGAELYNSASKTLEEVIEIIEDGVAAGKKIVRLHTGDPTIYGAIREQMDELERLNIA
                     YKIVPGVSSFTAACASIEREFTLPGVSQTVILTRIEGRTPVPEGEDLEELAKHQASMA
                     IFLSVQNIDRVVEKLAKGYGSYNIPVAVVYKASWQDEEIIKGTLKDIAGKVKERGINK
                     MAQILVGNFIEGEYERSKLYDAS"
BASE COUNT          371 a          158 c          250 g          260 t
ORIGIN      
        1 taatcaaaag atccatacca tatgggggca ggccccagag gatttaccgg ataaagaatt
       61 taataagtgt ttcatagggg gaagtagagg acagttggca ggaatttttt catatcttag
      121 ctcaaactta ctagaaggag gaatagtttg tgccaacttt ataactctag ataatttaaa
      181 tgagtttaat aaacttttat taaaatacaa ctataaagat atagagatca acctaataca
      241 ggcctctcaa gtaaataggc taggcatgtt aaaagggcag aatcctatat ttattataaa
      301 aggggtgaaa agcaatgctt agttttgtag gggcagggcc aggtcatgta gatttaataa
      361 cagtaaaagg taggcaacta ttagaagaag cagatattgt tatctatgca ggcagcctag
      421 tttcaaatga acatttaaag ttttgtaaaa agggggcaga gctttacaac agtgcctcta
      481 agaccttgga agaggtaata gaaataatag aggatggggt ggcagctggc aaaaaaatag
      541 taaggcttca tacaggagat cctaccattt atggcgccat aagggagcag atggatgaat
      601 tagaaaggct taatatagcc tataagatag taccgggagt cagctccttt acagcggcct
      661 gtgcttcaat agaaagggag tttaccctgc caggtgtaag ccagacagtt atcttaacta
      721 ggatagaggg caggacacct gtacctgaag gagaagattt agaagaactg gccaagcacc
      781 aggcctccat ggccatattc ttatcagttc aaaatataga cagggtagtt gaaaaattag
      841 ccaagggcta tggaagctac aatattccag tggcagtagt ctataaggcc agttggcaag
      901 atgaggaaat aatcaaaggc acccttaaag acatagctgg aaaggtaaag gaaaggggca
      961 ttaataagat ggcccaaatc ctggtaggta attttataga aggagaatat gaaagatcta
     1021 aactgtatga tgccagttt
//
LOCUS       DATGSI010000124         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_10040, whole genome shotgun sequence.
ACCESSION   DATGSI010000124 DATGSI010000000
VERSION     DATGSI010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10040"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1090
                     /gene="fliG"
                     /locus_tag="VK031_01895"
     CDS             83..1090
                     /gene="fliG"
                     /locus_tag="VK031_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HLS52695.1"
                     /translation="MRKRSNSVRKAAILLIALGPEISSQILKRLPDNFIQKVTYEIAN
                     IDYVEPGERDQVIEDFVDMVSAREYMLDGGIDYARDLLNKALGTQRAKEILDILSQLQ
                     QQERPFAIARKADPHQLTNLLVNEHPQTIALIMCYMQPDKAAMVLSQFPEELQIEIAE
                     RIGTINRTSPAVIKRIEEIMENKFSSLVESDTETVGGVRTLVNILNSVDRSTEKNIIG
                     DLEETQPELAEMVKANLFIFEDIVTLDRSSIQRILREVSNEDLALALKGASEEVSGII
                     FSNMSKRAAEMLKEDIKFMGPVRISAVEEAQHKIVGIIRRLDEAGEIVIARGEQDGII
                     L"
     gene            1074..1820
                     /locus_tag="VK031_01900"
     CDS             1074..1820
                     /locus_tag="VK031_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HLS52696.1"
                     /translation="MESSYRIIKNRNIANDKKAVSVINTKAVESYKKQETKPQKPEKA
                     EEIEKGPTEAEIRREIMMKIEEERISILKNAKEQADAVIAQAKKRGYSEGYETGYREG
                     LEEVRKEAAQIKEQALGLIRQAEEDVASYLEENRENIIRLSAQMAEAIIHQKIDSSDE
                     DLLLLIKPILERYEANGNIVITCHPSRVETMKEKASQLQLADGDARFIVLSDENMEEY
                     SCIIENQYQLIDLQIKKQLEAIVEEINKME"
     gene            1928..2588
                     /locus_tag="VK031_01905"
                     /pseudo
     CDS             1928..2588
                     /locus_tag="VK031_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
BASE COUNT          943 a          397 c          643 g          605 t
ORIGIN      
        1 aggctaaaga atttgccgaa gaaaatccag atgtggtggc agatctaatt agggcctgga
       61 taaaagacta gtaggtgaaa caatgagaaa gagatctaat agtgtaagaa aagcggctat
      121 tttattaata gccctaggac cagaaatatc gtcacaaatt ttaaaaagat tgccagacaa
      181 ttttattcaa aaggtgacct atgaaattgc caacattgat tatgtggagc ctggtgaaag
      241 ggatcaggtt atagaggatt ttgtggatat ggtctcagct agggaatata tgttggatgg
      301 tggcattgat tatgccaggg acctattaaa caaggcctta ggcacccaga gggccaaaga
      361 aattttagat atattaagtc aactgcaaca gcaggagagg ccctttgcta ttgccagaaa
      421 ggcagatcca catcagctaa caaatctgct tgtaaatgaa catcctcaga ccatagccct
      481 aataatgtgc tatatgcagc cagataaggc cgccatggta ttgtcccaat ttcctgagga
      541 gttgcagata gaaattgcag aaaggatagg gacaattaat aggacttcac cagcagttat
      601 aaaaaggata gaggagatta tggaaaataa gttttccagc ctggttgaaa gcgatacaga
      661 aactgttgga ggagtaagga ctttagtaaa catcttaaac tctgtagata gaagtactga
      721 gaaaaacatt attggggatt tggaagaaac acagccagag ctggctgaaa tggttaaggc
      781 aaacctattt atctttgaag atatagtaac cttagataga tcttccatcc agaggatatt
      841 aagagaagtt agcaatgaag atttagcctt agccttaaag ggtgcttctg aagaagtatc
      901 aggtattatc tttagtaata tgtctaagcg ggctgctgaa atgctgaaag aggacattaa
      961 atttatggga ccagtcagaa tctcagcagt agaggaggca cagcataaga tagtaggaat
     1021 aataaggcgg ctagatgaag ctggagaaat agtaatagcc agaggtgagc aggatggaat
     1081 catcctatag gattataaaa aatagaaata ttgccaatga caaaaaggct gtgtctgtaa
     1141 tcaataccaa ggcagtagaa agttacaaaa aacaggagac aaaacctcaa aagcctgaaa
     1201 aagctgaaga aatagaaaag ggaccaacag aggcagaaat cagaagagaa atcatgatga
     1261 aaatagagga agaaaggata agtattttaa aaaatgctaa ggagcaggca gatgctgtta
     1321 tagcccaggc caagaagaga ggttacagtg agggctacga aacaggctat agggaaggcc
     1381 tggaggaggt aaggaaagag gctgctcaaa taaaggaaca ggctttagga ctgataaggc
     1441 aggcagaaga agatgtggcc agttatttgg aggaaaacag ggaaaatatt attaggctat
     1501 ctgcccaaat ggcagaggca attatccatc aaaaaataga cagctctgat gaagatttgc
     1561 tacttttaat aaaacctatt ttagaaaggt atgaggccaa tggaaatata gtaattacct
     1621 gccatccatc tagggtggaa acaatgaagg aaaaggcctc ccagctacag ctggcagatg
     1681 gggatgccag atttatagtt ttaagcgatg aaaacatgga ggaatatagt tgtattatag
     1741 aaaaccaata ccagttgata gatctacaaa taaagaaaca gctggaagcc attgtagaag
     1801 aaataaataa gatggagtga aatctttgct aaatctacct tttgaagaat acggccaaat
     1861 aattaaaaga aaaaaatact atacaaccct aggtaaggta acccaggtta tagggctaat
     1921 aatccaggtg gaaggcctgg aaaccttcat aggagaggtt tgcgaagtct atataaaatc
     1981 ctctggtcca gagaaaactg tacttacaga agtggtaggt tttaaagata ctacagttct
     2041 tttgatgccc ctagatgaca ttaagggtat aggaccaggt tgtttagtaa aacctacagg
     2101 ccggtcccta aaggtaaaca taagtgagga actactaggc aaaactgtgg acgggctagg
     2161 tagaccagta gataaaaatg gtctaatcaa aggcgaagcc tatgatattg accgggagcc
     2221 accaagcccc tttgaaagaa gaaggattga aaaggtaatg agcacaggag tcaaggctat
     2281 agatgggctt ttaacctgtg gagaaggtca aaggataggc atctttgctg gttctggtgt
     2341 agggaaaagt acccttctag gtatgttggc tagacactgc gatgcagatg taaatgttat
     2401 aggcctgata ggtgagaggg ggcgggaggt tatagaattc atagaaaact ccctaggtga
     2461 ggaaggctat aaaaaatcta gtagtttgtg ccacctcaga tcaaccacct ttagttagac
     2521 taaagggagc ctttgtggcc acagccattg cagaatactt tagagaccag ggtaaaaagg
     2581 ttatgctc
//
LOCUS       DATGSI010000125         4309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_387612, whole genome shotgun sequence.
ACCESSION   DATGSI010000125 DATGSI010000000
VERSION     DATGSI010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387612"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1383)
                     /locus_tag="VK031_01910"
     CDS             complement(214..1383)
                     /locus_tag="VK031_01910"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HLS52697.1"
                     /translation="MILLDLKELKNKFIGLYGKGENIKTFFSPSRVNIIGEHIDYNGG
                     KVLPGAIGLGTYGIVRKREDNKVFLASENVDLRVEVNIDNLKFEKEHGWGNYPKGVLY
                     FIREVGYKIGGMDILVKGNIPYGAGLSSSASLELLIGEMVNILFNHGSIPTVELVKIC
                     QRAENEFVGVKCGIMDQFAIGMGKKEKVILLDTNNLQYEYIDMDLGDHILVIMNTNKQ
                     RELSDSKYNERREECEKGLDLIREYKDIEKLCDLSIEEFKELEEFIIPNDIKNRVEHV
                     VYENHRVNEAVTALKQGDILKIGQLLIESHNSLRDLYEVTGKELDSIVSSANKFPDCL
                     GARMVGAGFGGCAIAIVKKDSLDEFIDYVSKRYKENTGQDGEFYVTNISDGVREI"
     gene            complement(1380..2363)
                     /gene="galT"
                     /locus_tag="VK031_01915"
     CDS             complement(1380..2363)
                     /gene="galT"
                     /locus_tag="VK031_01915"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HLS52698.1"
                     /translation="MAELRWNPLLKDWTMVAANRQNRPDMPEDFCPFCPGSGKVPEDY
                     DVYKYDNDFPVLSQSPPKPDNVGNDFFKVREAYGKCEVILYSPDHNASLWELSVEHIK
                     KLVDLWIERFEELSKDKKIRYILIFENRGREVGVTMPHPHGQIYGYPFIPLKIQTELN
                     SSKEYFNENGQCLICKINEEEIKFKDRVIIENDDFIAYLPFFTDYPYGMYISSKNHIP
                     NLLYFTKKNRVNFAKILKDVTGTFDSLFNKEFPYMMVIHQSPVNTKEYKNHEEYYHFH
                     VEFYPPLRGENSIKYNASSETGAWAAANPRSLEETAVELKLAYERFKKDRR"
     gene            complement(2384..3364)
                     /gene="galE"
                     /locus_tag="VK031_01920"
     CDS             complement(2384..3364)
                     /gene="galE"
                     /locus_tag="VK031_01920"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HLS52699.1"
                     /translation="MAVLITGGAGYIGSHTVKYFSNKNEEIIIVDNLQSGYRKSIDNN
                     TFYNIDIRDRVEMDKVFKKHNIDGVIHFAANSLVGESMEKPYEYYHNNVYGMLCLLDT
                     MRNNKVDKIVFSSTAAVYGEPRNIPIKEDDMTLPTNPYGETKLAMENMMKWFDKAYGI
                     KYISLRYFNAAGAYEDGSIGEVHNPETHLIPLILQVPLGQREKIYIFGDDYPTEDGTC
                     IRDYIHVMDLASAHYLAFKYLKNGNKSDIFNLGNGNGYSVKEVVETARRVTGHPIPAE
                     VAERRPGDPAVLVASSKRIKEVLNWRPKFESLEKIIEDAWRFHRKNPTGY"
     gene            complement(3383..4258)
                     /gene="galU"
                     /locus_tag="VK031_01925"
     CDS             complement(3383..4258)
                     /gene="galU"
                     /locus_tag="VK031_01925"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HLS52700.1"
                     /translation="MKIRKAVIPAAGLGTRFLPATKAQPKEMLPIVNKPALQYIVEEI
                     VDAGIEEILIITGRNKYSIENHFDRAMELEWELEKKRDLELLKDIKRISNMAKIHYIR
                     QREPLGLGHAIYCAKTFIGDEVFAVLLGDDIIHSQRPCIGQLIEVYEKYKGSILGVKK
                     MNKDVLHKYGNLLGRELEEGVYRVKKLVEKPKLEEICSNVSVLGRYIITPSIFNILKK
                     LSPGIDGEIQLTDGLNKLAQMEPVYAYEFEGKRYDIGDRQGFLKANIEFALRDESLKD
                     GLIKYLSDILDSYHI"
BASE COUNT         1270 a          900 c          496 g         1643 t
ORIGIN      
        1 gaagagtgga aaattgtctt aacctttgct tttattccac tgattattgg agaattatat
       61 aaatttgtac caaacaaata aagggcttgc cctttatttg tttttgtcat cttcctagac
      121 acatattttt tactgccagc aagtaaactc ttatttcaca gaactccaaa ttattttaca
      181 ttaccaaaga aatatagagt ctaggcctag actctatatt tctctcactc catcactgat
      241 attagttaca taaaactctc catcctgtcc tgtgttttct ttatatcttt tacttacata
      301 atcaatgaat tcatctaagc tatccttctt aactatggca atagcacatc caccaaaacc
      361 tgcccctacc attctagctc ccaaacaatc agggaattta ttggcactag atactatact
      421 atctaattct ttgccggtaa cctcatataa gtctcttaat gagttatgag attctataag
      481 taattgtcct atttttaaaa tatccccctg ttttaaagcc gttacagctt catttactct
      541 gtgattttca tatactacat gttctaccct atttttaata tcattgggaa taataaattc
      601 ttctagttcc ttaaattctt ctatacttaa atcacaaagc ttttctatat ctttgtattc
      661 tcttattaag tcaagtccct tttcacattc ttctcgtctt tcgttatatt ttgaatcact
      721 taactcccgt tgcttattag tattcataat tactaaaata tgatctccta aatccatatc
      781 tatatattcg tattgaagat tattagtatc taataagatt actttctcct ttttacccat
      841 acctatggca aattgatcca ttattccaca cttaactcca acaaattcat tctctgccct
      901 ttgacatatt ttaactaatt caacggtagg aatacttcca tgattaaata aaatatttac
      961 catttctcca attaataatt ctaaagaagc cgacgaagat aggcctgccc catagggtat
     1021 attccctttc actaatatat ccataccgcc tattttgtat ccaacttccc ttataaaata
     1081 taatacgccc tttggataat ttccccaacc atgttctttt tcaaacttta gattgtctat
     1141 attaacttct actcttaagt ctacattctc tgaggctaaa aagactttat tatcctctct
     1201 ttttcttact atgccataag ttcctaatcc aatagcccct ggtaaaactt tccctccatt
     1261 ataatcgata tgctctccta ttatattgac tctacttgga gaaaaaaagg tctttatatt
     1321 ttcgcctttt ccatataatc caataaactt atttttcaac tcctttaaat ccaataaaat
     1381 catctcctat cctttttaaa tcgttcatat gctagtttaa gttctactgc agtctcttct
     1441 aagcttctag gatttgctgc cgcccaagct cctgtttctg atgatgcatt atattttatg
     1501 ctattttccc ctcgtaaagg tggataaaat tctacatgga aatgataata ctcttcatga
     1561 tttttatatt ctttagtatt tacgggactt tggtgaataa ccatcatata aggaaattcc
     1621 ttattaaata aagagtcaaa ggtgccagtt acatccttta agattttagc aaaattaact
     1681 ctatttttct tagtaaaata tagaagatta ggaatatggt ttttagaaga tatatacatg
     1741 ccataaggat agtcagtgaa aaagggtaaa taagctataa aatcatcatt ttctattatt
     1801 actctatcct taaacttaat ttcttcttcg ttaattttgc aaattaaaca ctgtccattt
     1861 tcattaaagt attcctttga tgagtttaat tctgtctgga tttttaaagg tataaaggga
     1921 tatccatata tttgtccatg aggatggggc atagttactc ctacttccct acctctattt
     1981 tcaaaaataa gaatatatct tatcttttta tcctttgata attcttcaaa tctctctatc
     2041 cataaatcta ccagcttctt tatatgctct acacttaatt cccataagga agcattatgg
     2101 tctggtgaat ataatattac ttcacatttg ccataggctt ctcttacttt gaaaaagtca
     2161 ttgcctacat tatctggttt tggtggactt tgggacaaaa cgggaaaatc attatcatat
     2221 ttatatacat cataatcttc tggaacctta cctgaacctg ggcaaaatgg acaaaaatcc
     2281 tcaggcatat ctggcctatt ttgcctatta gctgccacca tagtccaatc ctttaataag
     2341 gggttccatc ttaattctgc cattaatctt acctcccttt atgttagtat cctgtaggat
     2401 ttttcctatg aaatctccat gcatcttcta ttattttctc taaagattca aatttaggtc
     2461 gccaatttag aacttcctta atccttttag atgatgctac caaaacggcc ggatctccag
     2521 gtcttctttc tgctacttcc gcaggtatcg gatgtcctgt tactctccta gcagtttcta
     2581 ctacctcttt tactgaataa ccattgccat ttccaaggtt aaatatatcg cttttatttc
     2641 cattcttcaa atatttaaag gctaaataat gggctgaagc caaatccata acatgaatat
     2701 aatctctgat acaagtacca tcctctgttg gataatcatc accaaatata tatatctttt
     2761 ccctttgtcc taaaggtact tgaagtatta aaggaattaa atgggtctct ggattatgga
     2821 cttctcctat acttccgtct tcatatgctc cagctgcatt gaagtatcta agggaaatat
     2881 atttgatacc ataggcctta tcaaaccatt tcatcatatt ttccatagct aattttgttt
     2941 ccccatatgg attagtaggc aaagtcatat catcttcctt tatagggata tttctaggct
     3001 ctccatatac tgctgctgta gatgaaaata ctattttgtc caccttatta tttctcattg
     3061 tatctaataa gcataacatc ccatatacat tattatgata atattcatag ggcttttcca
     3121 tactctcacc aactaaagaa ttagcagcaa aatggataac accatctata ttatgttttt
     3181 taaatacctt atccatttct accctatctc taatatctat attataaaaa gtattattat
     3241 ctatggattt tctatagcca ctttgtagat tgtctactat tattatttcc tcgtttttgt
     3301 ttgaaaaata ctttaccgta tgggatccta tatatccagc tccccctgtt attaaaaccg
     3361 ccatagtctt ccctcctaat aattaaatat gatatgaatc caaaatatca cttaaatatt
     3421 taattaatcc gtcttttaag ctctcatccc ttagcgcaaa ttctatattt gccttcaaaa
     3481 atccctgcct atctccaata tcataccttt tgccttcaaa ctcataagca taaacaggct
     3541 ccatttgtgc cagcttattt aatccgtctg ttaattgtat ttctccatct ataccaggtg
     3601 acaatttttt taatatatta aatatactgg gggtaataat ataccgacct aaaactgaaa
     3661 cattggaaca gatctcctct aatttaggct tttctactaa ctttttaacc ctatataccc
     3721 cttcttccaa ttctctccca aggagattac catatttatg caatacatct ttattcattt
     3781 tttttactcc taagatgctt cctttatatt tttcatacac ttctattaat tgtccaatac
     3841 atggcctttg agaatgtata atatcgtccc ctaaaagaac agcaaagacc tcatctccaa
     3901 taaatgtttt tgcacaatat atggcatgtc ccaatcctaa aggctccctt tgtctgatat
     3961 agtgaatttt agccatattg gatattcttt ttatatcttt caataattct agatcccttt
     4021 tcttttcaag ttcccattct aattccatag ccctatcaaa atgattttct atggagtatt
     4081 tatttctacc tgttataatt aaaatttctt ctataccagc atctactatt tcctccacta
     4141 tatactgtaa ggctggctta tttactatgg gtaacatttc tttcggttgt gctttggtgg
     4201 cgggcaagaa tctagttcct agtcctgcag ctggaataac cgcctttctt attttcatta
     4261 catccacctc ttacttagat ctacattatc atgtatattg attatatca
//
LOCUS       DATGSI010000126         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_104489, whole genome shotgun sequence.
ACCESSION   DATGSI010000126 DATGSI010000000
VERSION     DATGSI010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104489"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VK031_01930"
     CDS             <1..192
                     /locus_tag="VK031_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52701.1"
                     /translation="FKMYNSQINTVNKFSFAGIVEFHRKRGASDAQKEVRDLADMMID
                     VVRNIEGNPFKYSLEVYGF"
     gene            204..470
                     /locus_tag="VK031_01935"
     CDS             204..470
                     /locus_tag="VK031_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52702.1"
                     /translation="MFKVALKIPVYLEKEGSQELAKLGIGVEGLGDIDYVYVWTLLEC
                     FDHELDGVTGTLFTTGSGGGLFSPLKIDNFIKMVDDHIEKYGRG"
     gene            470..778
                     /locus_tag="VK031_01940"
     CDS             470..778
                     /locus_tag="VK031_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52703.1"
                     /translation="MKRLDVEIKMRGFTYKLLRRGERVALYSQFCDDSGKLVGYEMFE
                     IPTRSPETFKGKEYPAREVYPPTSRWGRNAFTLSKFSKIEKVLKRFEEYERDIAQTDN
                     "
     gene            835..1026
                     /locus_tag="VK031_01945"
     CDS             835..1026
                     /locus_tag="VK031_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52704.1"
                     /translation="MYDKIVKERYESQTKWWDDEMLEEYGDYILEDAREYAEGYMDGY
                     KDALREEDRKKHETEIKDV"
     gene            1001..1414
                     /locus_tag="VK031_01950"
     CDS             1001..1414
                     /locus_tag="VK031_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HLS52705.1"
                     /translation="MKQKLKTCSNCGNDFPRLWKAKTRDHGAMCKDCWNGYKAGEVSI
                     DKSKRKAPKRIKPISDKMAKKLVEYRKLRDKYLKENPNCEICGSSGVELHHKRPRAYH
                     LTNVDVFMSVCRKCHERIEREDKWARGNGYKIDDL"
     gene            1584..1895
                     /locus_tag="VK031_01955"
     CDS             1584..1895
                     /locus_tag="VK031_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52706.1"
                     /translation="MTILLITVFIVATVLLAWSLLKDMFKSSKIEQLSIHARHDDKGE
                     PVDEQELKIAMLIQIATELYDRDYIDIEIKRSGETGWIIESDVLLPNITFTQIKRATD
                     K"
     gene            1940..2101
                     /locus_tag="VK031_01960"
     CDS             1940..2101
                     /locus_tag="VK031_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52707.1"
                     /translation="MAKSCGFADFLDLITKASKGEISEYDLRVAFERLAEDVKVETEF
                     EYKMEEYGI"
     gene            2091..2453
                     /locus_tag="VK031_01965"
     CDS             2091..2453
                     /locus_tag="VK031_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52708.1"
                     /translation="MEFKGELEGFPKEVVERMLFWQENQTGERDISVFEEGVSTHSCR
                     GGFNWEDTIEGYEFWEDVICNKNFGRFYEITGGEVKEITKEPMRCYIDLGGGNKVEFL
                     IDGLDINFDKGFIRIKAK"
BASE COUNT          989 a          308 c          630 g          683 t
ORIGIN      
        1 tttaaaatgt acaactctca aattaacact gtaaataagt tttcattcgc aggtattgtg
       61 gagtttcaca ggaagagagg tgcatcagat gctcaaaagg aggttagaga tttagccgac
      121 atgatgattg atgtagtaag gaatatagag ggtaatcctt ttaagtattc tttagaggta
      181 tatggatttt aaaaataaaa gatatgttta aggtagcttt aaaaatacca gtgtatctgg
      241 aaaaggaagg ttcacaagaa ttagcgaaac taggtatagg agtagaagga cttggagata
      301 tagactatgt gtacgtatgg accttgttag aatgttttga ccatgaatta gacggtgtga
      361 caggaactct attcacaact ggaagtggag gaggattatt cagtcctcta aaaatagaca
      421 actttataaa aatggtagat gaccatatag aaaaatacgg aaggggataa tgaaaaggtt
      481 agatgtagag ataaaaatga gagggtttac ttataagctt ctgagaagag gtgagagagt
      541 agccctctac tcccagttct gtgatgattc tggaaaacta gtagggtacg aaatgtttga
      601 gattccgaca aggtcaccag aaacatttaa aggaaaggaa tatcctgcta gagaggtgta
      661 ccctccaaca agcaggtggg gaagaaacgc attcactctt tccaagttct ctaagattga
      721 aaaagtatta aagagatttg aggagtatga acgagatatt gcacaaactg acaattaatt
      781 aaaatattac atattatgag aatagatgaa tttttaaaag tacaagagga atccatgtac
      841 gacaaaatag ttaaagagag atatgaatcg caaaccaagt ggtgggatga tgaaatgctt
      901 gaggagtacg gagactacat attagaagat gccagagagt atgccgaagg atatatggat
      961 ggatataagg atgcactaag agaagaggat aggaagaaac atgaaacaga aattaaagac
     1021 gtgtagtaac tgcggtaacg acttccctag gttgtggaaa gctaagacta gagaccacgg
     1081 agctatgtgt aaggactgtt ggaatgggta taaagcagga gaggtaagta tagacaaatc
     1141 aaaaagaaaa gctcctaaga gaattaaacc tatatccgac aaaatggcta agaaactagt
     1201 ggaatacaga aagcttagag ataaatacct aaaggaaaat cctaattgtg agatatgtgg
     1261 ttcttcgggc gtagaattac accacaagag accaagagca taccatttaa caaacgtaga
     1321 tgtatttatg agtgtgtgca ggaaatgtca cgagagaatt gagagagaag ataaatgggc
     1381 aagaggtaac ggatataaaa tagacgacct ttaactcatg acaaataaat gacagagttt
     1441 aaataaaaag agtttatatt tgtagtataa attgattaat taaaaaggat tggttgaggg
     1501 gttttaacac caaagtgaac agaatggttg gaagagtata gaaagtgtct aaaatcccac
     1561 acctttccac aaataaagaa gatatgacaa tattattgat aacggtattt atagtagcaa
     1621 cagtattact cgcatggagt ctgttaaaag atatgtttaa gagttcaaaa attgagcagt
     1681 taagtatcca tgctaggcac gatgataaag gtgagcctgt agacgagcaa gaactgaaga
     1741 tagctatgtt aattcaaatt gccactgagc tatatgacag agactatata gacatagaaa
     1801 ttaaaaggag cggtgagact ggttggatta ttgaatcaga cgttctactc cctaatataa
     1861 catttactca aataaaaaga gcaactgata aataaaaaga gatatgaatt attttgataa
     1921 cttagaatta tttgattcca tggctaagag ttgtggattt gcagacttcc ttgatttaat
     1981 tacgaaggct tctaaaggag aaataagtga atatgattta agggtagcgt tcgagagatt
     2041 agcggaggac gtcaaagtag aaaccgagtt tgagtacaaa atggaagaat atggaattta
     2101 aaggtgagtt agaaggattc cctaaggaag ttgtagagag gatgcttttc tggcaagaga
     2161 accagacagg agaaagagac ataagtgtgt ttgaggaggg tgtatctacc cactcctgta
     2221 gaggagggtt taattgggaa gatactattg agggttatga gttttgggaa gatgttatat
     2281 gtaacaaaaa ctttggtagg ttctatgaga ttacaggggg tgaagtcaag gaaataacta
     2341 aagagccaat gaggtgctat atagacttag ggggtggcaa caaggtagag ttcttaatag
     2401 atggcttaga cattaacttc gataaaggtt ttattaggat aaaagctaag tgattatgga
     2461 agaattaaga aagtatattg gagataaaga aaaagcgtat atcaggacta gaactcaatt
     2521 tgaagctgtc agactcttaa ccaatttaga tacactagga tacagttgga ttgatggaga
     2581 tactatagat gttgaagata caggttgggt
//
LOCUS       DATGSI010000127         4619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_152226, whole genome shotgun sequence.
ACCESSION   DATGSI010000127 DATGSI010000000
VERSION     DATGSI010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152226"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VK031_01970"
     CDS             <1..916
                     /locus_tag="VK031_01970"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HLS52709.1"
                     /translation="YVDAIRHSYWVHDEFDYTSDIQDMMINLSEEDKSIVTRAMLAIS
                     QIENTVKMFWGNVYHHIPKPEIASVGATFSDSEARHQDAYSHLLELLGLQKEFESLLD
                     VPVMADRVRYLTEANEKSNSTDPKEYFESLILFSMMIENVSLFSQFFIIMSYNKHANM
                     LKGMSNVIEATSKEEACHFQFGADLINIIKKENPQWWTEDLKQRVLDFVSRAGYAEGQ
                     VVDWIYEKGDSSIIPKSQVHDYIGYKVAQSLESIGIPCEDKFFEDELSNFKWFEEEIV
                     LTKNHDFFNKRSTAYNKMSQTITADSIF"
     gene            925..1671
                     /locus_tag="VK031_01975"
     CDS             925..1671
                     /locus_tag="VK031_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52710.1"
                     /translation="MFEWLNKDSRKFLARGYLKNDQLPEERIKEITETFRGYIGSKEI
                     AYKFYKYLGKGYYSLSSPVWSNYGTNRGLPVSCFGSYIEDSTASILSAHSEIGIMNKM
                     GGGAAAYFGNVRGRGSPIGQDKGESFGSVHFMEMFDNLVKVISQGKVRRGAFSPYLPI
                     EHPDALEFIRIGEEGHPIQGLTHGVTVTNEFIEKLYSGDLEARTKWAEVIKARGERGY
                     PYIIFIDNINDNTVGVYKDKNRKIVASNLC"
     gene            1778..2440
                     /locus_tag="VK031_01980"
     CDS             1778..2440
                     /locus_tag="VK031_01980"
                     /inference="COORDINATES: protein motif:HMM:NF015141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52711.1"
                     /translation="MDKKQLVKLVSSFSMADGGLYKRSPNGNAYFMMNMLTEHMDYIE
                     WVQDTLEHLTKTSVSKIKNPYKNPQTRICSRTLPFFTKIHGRVYKGNYKSLDPHALKM
                     LDWEMLSIFHMADGCLYVEQPNPKKGLVNPSPNISLNMKRLSYGDIWFLKKTIKDRLG
                     IEYNIGQQNYRGKTYYFLRLRNKDVKRFSEGVLPYTCPSFYYKVYDSVRKAPVQQDDD
                     IV"
     gene            2472..3431
                     /locus_tag="VK031_01985"
     CDS             2472..3431
                     /locus_tag="VK031_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52712.1"
                     /translation="MILPIPSIVTKLSEIALPSSPTESFVCVLASMNLLHYEEWKDTD
                     AVRVLTYFLDTVVTDFLESLDRLHKTDRDAALYMHRAYNFAKKHRALGLGALGWHSYL
                     QSNHLSFESQEAAKKNLEIHKHIEEHSWNASRELSQLFGEPELLAGYGRRNTTLTAIA
                     PTKSSSFILGQVSPSIEPYVSNYFVDDKAKIKVTFKNPYLKSALESVYGKDTEEVWES
                     IRKHDGSVQHLDFLTDYDKEVFKTYGEINQEAIINQAAVRQDYIDQSQSLNLMIGPTM
                     SAKEINRLILHAHKLGIKSLYYQHSVSAAQELVRNTSCVACEG"
     gene            3433..3663
                     /locus_tag="VK031_01990"
     CDS             3433..3663
                     /locus_tag="VK031_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52713.1"
                     /translation="MNTIDKEAILEIADSQKDMIRKQYRETLIDRIIDRAALGYYHLR
                     MEPLPGWAQRDLEVSGFTVVVQPREATIWWDE"
     gene            3660..4121
                     /locus_tag="VK031_01995"
     CDS             3660..4121
                     /locus_tag="VK031_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52714.1"
                     /translation="MSIKPDQLRKYVIQPALRYIGAYSEDAEELLILTAAQETHLGQD
                     LHQVNGPALGIFQMEPATAQDIYDNFLIYRPHLLTLVSDLAIGQHVEDELCGNLYYAA
                     AMARCTYLRHSEPLPDKNNLRGMAEYYKKYYNTVKGAAKLEDIFENYHRYT"
     gene            complement(4206..>4619)
                     /locus_tag="VK031_02000"
     CDS             complement(4206..>4619)
                     /locus_tag="VK031_02000"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLS52715.1"
                     /translation="MVGTESAFGTSNAMSRAGAVGVTQLMPDTARDMGLHVPKEAGTN
                     SNPNLNYIPGPDDERMDNEKALEASAKYVKGLLKHYNNDPVLAYASYNWGQGNMDKYL
                     AGSLNTIPDETEEYIKKIFHTSLSQYIQSKGLRKQ"
BASE COUNT         1474 a          822 c         1042 g         1281 t
ORIGIN      
        1 atatgtagat gctattaggc attcttattg ggtacatgat gaatttgatt atacttctga
       61 cattcaggat atgatgatca atctatcaga ggaagataag tctattgtta cccgtgccat
      121 gcttgctatt tcacagatag agaatactgt caagatgttc tggggaaatg tgtatcacca
      181 tatacctaaa ccagagattg ctagtgtagg cgctacattc tcagactcag aggcgaggca
      241 ccaagatgca tattcccacc tgttagagtt gttaggtctt cagaaggagt ttgagagctt
      301 gctggatgtt cctgttatgg cggacagggt gaggtatttg acagaggcta acgagaagtc
      361 taactctact gacccaaagg aatactttga atcactcatt ctgttctcta tgatgataga
      421 aaacgtttcc ttatttagtc agttctttat cattatgtcg tataacaaac acgccaacat
      481 gttgaaggga atgagtaatg ttattgaggc gacatccaag gaggaagcgt gccatttcca
      541 gtttggtgct gatttaatta acatcattaa gaaggagaac ccccagtggt ggacagagga
      601 cttgaagcag cgtgtcttag attttgtgtc tagggctggc tacgcagagg gacaggtagt
      661 agactggata tatgagaaag gggattcttc tataatccct aaaagtcagg tgcatgatta
      721 tatagggtat aaggttgcac aatctttaga aagcataggc ataccctgcg aggacaaatt
      781 ctttgaagat gaactaagca acttcaagtg gttcgaggag gagatagtcc tcaccaagaa
      841 tcacgacttc tttaataaga gaagtaccgc ctacaacaag atgtcccaaa ctatcacagc
      901 agacagtatc ttttaggagt atcaatgttt gaatggctta ataaggattc ccgaaagttt
      961 ttggctaggg ggtacttgaa gaacgaccag ttgcctgaag aaagaatcaa agagataact
     1021 gaaaccttcc ggggatacat aggaagtaaa gagatagctt ataagttcta taaatactta
     1081 ggtaaaggat attacagctt gtcctccccc gtgtggtcta attatgggac aaacaggggg
     1141 ctaccggtgt catgttttgg aagctatata gaagatagca cagcctccat cctgtctgct
     1201 catagtgaaa taggtatcat gaataagatg gggggagggg cagcggccta cttcggaaat
     1261 gttagaggaa gaggaagccc tataggacaa gacaagggag aatccttcgg atcagttcac
     1321 ttcatggaaa tgtttgacaa tctagtcaaa gtaattagtc agggaaaagt taggagggga
     1381 gcattcagtc catatcttcc tatagaacat cctgatgctc tggagtttat cagaatagga
     1441 gaggaggggc atcccattca gggattaact cacggagtaa cagttactaa cgagtttata
     1501 gagaagctat actctgggga tttagaggcc agaaccaaat gggcagaagt aattaaggct
     1561 agaggagaaa gagggtatcc ttatatcata ttcattgata atatcaatga taatactgta
     1621 ggtgtgtaca aggataagaa taggaagatt gtagcaagca atttgtgtta actagtagca
     1681 cctttcgtaa gtaattacga tagaaaactt tcctaaaaac ggggaaactc cttaagggac
     1741 aatcccgtgc caaaccgata tggtaggtgt aacgactatg gataaaaaac aacttgtaaa
     1801 attagtatct tctttcagca tggctgatgg aggactgtat aaaagaagcc ccaacggtaa
     1861 tgcatacttt atgatgaata tgcttactga acatatggat tatatagaat gggttcagga
     1921 cactttagaa cacttaacta agacttcagt ttctaaaata aaaaatccct ataagaaccc
     1981 tcaaactaga atttgctcca gaacccttcc gttcttcacc aaaatacatg gaagggttta
     2041 taaaggaaac tataaaagcc tagaccctca tgcactaaaa atgcttgact gggaaatgct
     2101 ttctatattt catatggccg acgggtgtct ctatgtggaa caacctaatc ctaaaaaggg
     2161 attagtaaac ccctctccta atatatctct taatatgaaa agattgagtt atggagatat
     2221 ttggttccta aagaagacaa ttaaagatcg gctggggatt gaatataaca taggacagca
     2281 gaactacaga ggtaagactt attacttcct caggttacgt aacaaagacg ttaaaaggtt
     2341 ttctgaggga gtactacctt acacctgccc tagcttttat tacaaggttt atgattccgt
     2401 acggaaagct cctgtacagc aggatgatga tatagtctga gctgcatggt aacatgcaga
     2461 ggaggacaga aatgatcctc cctataccta gtatagtaac aaaactgtca gagatagccc
     2521 tgccttcttc tcctacagag tcctttgtgt gcgtattggc atcgatgaat cttctgcatt
     2581 atgaggagtg gaaagataca gatgcggtaa gggttcttac ttatttcctt gatactgtag
     2641 ttacagattt ccttgagtca ttagacagac tgcacaagac agatagagat gcagcactat
     2701 acatgcaccg agcgtataac tttgctaaga aacatagagc attaggtctg ggggcgttag
     2761 gatggcatag ttatctacaa tctaatcatc tttcatttga atcgcaagag gctgctaaga
     2821 agaatcttga aatacataag cacattgaag aacattcttg gaatgctagt agagagttgt
     2881 cgcagctctt tggagagcca gagctactgg ctgggtatgg cagacggaat actactctca
     2941 ctgctattgc tcctactaag tcaagcagtt ttattttagg gcaagttagt ccttctatcg
     3001 aaccgtatgt aagtaattac tttgtggatg ataaggctaa gattaaagta accttcaaga
     3061 atccttacct gaagtctgca ttggaatcag tatacggtaa agacacagag gaagtgtggg
     3121 agtctatcag gaagcatgat ggttcagtac agcacttgga ttttctgaca gactatgata
     3181 aggaagtatt caagacttat ggagagatca atcaggaagc aataattaat caggcagcag
     3241 taagacagga ttatatagac cagtcacaga gcttgaatct tatgattggt cctactatga
     3301 gtgctaaaga gatcaatagg ttaattcttc atgctcataa gctggggatt aaatcattgt
     3361 attatcaaca ctcggtatct gctgctcagg aattagttag aaatacttct tgtgtagcat
     3421 gtgaaggatg atatgaatac aattgacaag gaagctatac tagagatagc agattcccaa
     3481 aaggatatga taaggaaaca gtatagggag acactgatag acaggatcat agacagggca
     3541 gctttaggat attatcatct gagaatggaa cctttgcccg ggtgggcaca aagagatttg
     3601 gaggttagcg gatttaccgt ggtggtacag cctagagaag caacgatatg gtgggatgaa
     3661 tgagtattaa gccagaccaa ctaaggaaat atgtaataca gcccgctttg agatacatag
     3721 gggcgtattc tgaagatgca gaggaacttc ttatactaac agcagcgcag gaaactcatc
     3781 taggtcagga tctacatcaa gtaaatggac cggcattggg tatattccaa atggaacctg
     3841 ctaccgcaca ggatatctat gataatttcc ttatctatag accgcatctc ctgacattgg
     3901 tgagtgatct agctatagga caacatgtag aggacgaact gtgcggtaat ttatattatg
     3961 ctgccgctat ggcacgatgc acatatttaa gacactcgga acccctacct gataaaaata
     4021 atctcagagg aatggcagag tactataaaa aatattataa cacagtgaaa ggagcagcta
     4081 agttagaaga tatatttgag aattatcata ggtatactta gtaacctatt actgtagaca
     4141 aaaagaaacc ccgtagggaa ttaaccttac ggggtatttt aatggttatt atttttatgt
     4201 tagtattatt gtttccgcag tcccttgctt tggatatatt gagataagct agtatggaag
     4261 atcttcttaa tgtactcctc agtctcgtct ggtatagtat tcaaagaacc tgctagatac
     4321 ttatccatgt taccttgccc ccaattataa gaggcatagg ctagtaccgg atcgttatta
     4381 taatgcttca gcaatccctt aacatactta gcggaggctt ctagggcttt ttcattgtcc
     4441 atcctttcat catctggtcc gggtatatag ttgagattag gattactatt agttcctgct
     4501 tccttgggaa catgaagccc catatcccta gctgtatctg gcatcaattg agtaacacct
     4561 actgcccctg ccctactcat agcattagaa gtaccgaagg cagactcagt acctaccat
//
LOCUS       DATGSI010000128         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_387775, whole genome shotgun sequence.
ACCESSION   DATGSI010000128 DATGSI010000000
VERSION     DATGSI010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387775"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VK031_02005"
     CDS             <1..1680
                     /locus_tag="VK031_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HLS52716.1"
                     /translation="EEEILNERIYGVVPNFDLEKLLDSKEREPIPIKYDDEHYLVFPN
                     FVDNKKIQSIRNKIIILYWVNNTDFVKLKQLYKDETAILSLVYIDNYDDVKNSTPDIN
                     RPILLAEIDKRITEYFSEYNGVVRKFENDKYLVIIDNKALEDIQEKRFDLLDNIRELD
                     YGNKIPITLSIGVAATEGNLLESHNNARAAIDIALGRGGDQAVLNRNGTLDFYGGKSK
                     ALEKRNKVRSRVIGYALRQLIDQADNVFVMGHKNPDMDSFGSALGVLRGVQNRKKDGY
                     IVLGKETTSIKNIMARLEQEQPELLDKIITAEEAEELIKENSLMVLVDNHKPSFTEAP
                     ELLDLIDSVVIIDHHRRGAEFVKDPVLIYLEPYASSTSELVTEILSYMSDNTNLTKFE
                     AEALLAGITVDTKNFSFQTGVRTFEAASILKRAGADTTAVRQLFREDHDTFIYKASVI
                     KSSKVIRDTIVVGRLEEELDNGVLIAAQAANELLDINGIEVSFVLTYANDRVHISGRS
                     LGNISVQLILENLGGGGHLTSAGVQLTGINLDDAEIKLMEAIDKYFGEGES"
     gene            1680..2126
                     /gene="rplI"
                     /locus_tag="VK031_02010"
     CDS             1680..2126
                     /gene="rplI"
                     /locus_tag="VK031_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HLS52717.1"
                     /translation="MKIILLEDVKGLGKAGELVNAKAGYARNFLFPKNLAIEATPANL
                     KKWKEEMKQKEAREEEERQEALKLKERIEKETVTLKAKGGEGGRLFGSITSKDIADAL
                     KKQHKIDIDRRKIELKDNIKEAGITNVDVRVYPEITASLKVNVIIE"
     gene            2141..>2381
                     /locus_tag="VK031_02015"
     CDS             2141..>2381
                     /locus_tag="VK031_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HLS52718.1"
                     /translation="MEAQAIGRIPPHSIEAEQSVLGAMIKDKLAINTAVEIIRPEDFY
                     REANGEIYEAILDLFNKGEPVDLITLSEELKRRGTL"
BASE COUNT          890 a          313 c          530 g          648 t
ORIGIN      
        1 gaggaagaaa tattaaacga aagaatctat ggtgtggtac ctaattttga tttagaaaag
       61 ctacttgatt caaaggaaag ggagccaatt cctattaaat atgatgatga acattatctg
      121 gtgtttccaa attttgtaga caataaaaag atacaaagta ttagaaataa aataatcata
      181 ttatattggg taaataatac tgattttgta aaacttaagc agttatataa ggatgaaact
      241 gccatattat ctctagttta tatagataac tatgacgatg taaaaaatag tactccagat
      301 ataaataggc ctattttatt ggcagaaata gataagagaa ttacagaata tttttcagaa
      361 tataatgggg tagtaaggaa atttgaaaat gataaatacc tggtaatcat agataataag
      421 gctttagaag atatccagga gaaaagattt gatcttttag ataatataag agagttagat
      481 tatggaaata agattcctat aaccttaagt ataggggttg cagctacaga aggaaaccta
      541 ttggaatccc ataataatgc aagggctgcc atagatattg ccctaggtag aggtggagac
      601 caagcagttt taaataggaa tggtacatta gatttctatg gtggaaaatc aaaggccttg
      661 gaaaaaagaa acaaggttag atccagggta ataggttatg ctcttagaca attaatagat
      721 caggccgata atgtttttgt tatgggtcac aaaaatccag atatggattc ctttgggtct
      781 gccctaggag tattaagagg tgtacaaaac cgaaaaaaag atggttatat agttttaggt
      841 aaagaaacca catctattaa aaatattatg gccagattgg agcaggagca accggaacta
      901 ttagataaaa taattacagc agaggaagca gaggaattaa taaaggaaaa ttccctaatg
      961 gttttggtag acaatcataa gcctagcttt acagaagcac cagaactatt agacttaata
     1021 gatagtgtgg taataataga tcaccataga agaggggctg aatttgttaa agatccagtg
     1081 cttatttacc tggagcctta tgcttcgtct accagtgaac tggttactga aattttaagc
     1141 tatatgagtg ataatacaaa cctaaccaag tttgaagcag aagccctatt ggcaggtatt
     1201 actgtagata caaagaattt tagtttccaa actggtgtta ggacctttga ggctgcttct
     1261 attttaaaaa gagcaggagc agatactact gctgtaagac aattatttag ggaagatcat
     1321 gataccttca tatataaggc cagcgttata aaatcatcta aggtaatccg ggataccata
     1381 gtggtaggta gattggaaga agagttggat aatggagtat taattgcagc tcaggctgcc
     1441 aatgaacttc tagatataaa tggtatagag gtgtcctttg tacttaccta tgccaatgat
     1501 agggttcaca tttctggcag gtctttaggc aatatttcag tccaactaat attagaaaac
     1561 ttaggcggtg gaggacacct aaccagtgca ggtgtccaat taacaggcat taatcttgat
     1621 gatgcagaga taaaacttat ggaagcaata gataaatatt ttggggaagg tgaatcataa
     1681 tgaaaattat cttattagaa gatgtaaaag gtttaggcaa ggctggagaa ttggtaaatg
     1741 ctaaggcagg ttatgctagg aattttctat ttcctaaaaa tcttgccata gaggctactc
     1801 cggctaactt gaaaaagtgg aaggaagaaa tgaagcagaa ggaagctaga gaagaagagg
     1861 aaaggcaaga agccttgaaa ttaaaggaaa ggatagagaa agagactgta accttgaagg
     1921 ctaaaggtgg agaaggtggt aggctatttg gctccattac ctccaaggac attgctgatg
     1981 ctttaaagaa acaacataag atagatatag atagaagaaa aatagaatta aaagacaata
     2041 taaaagaagc tggaataacc aatgtggatg taagagtata tcctgaaata actgccagct
     2101 taaaggttaa tgtaattata gaatagttgg tgataaaggt atggaagccc aagcaatagg
     2161 acgcattcca ccccatagta tagaagctga gcaatctgtc ttaggggcaa tgataaagga
     2221 taaactggcc attaatacag ctgtagaaat cataaggcct gaggactttt acagagaggc
     2281 caatggtgag atttacgagg ccatattaga cttatttaat aagggtgagc cggtggattt
     2341 aattacccta tctgaggaat taaaaagaag gggcacccta g
//
LOCUS       DATGSI010000129         1099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_104898, whole genome shotgun sequence.
ACCESSION   DATGSI010000129 DATGSI010000000
VERSION     DATGSI010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104898"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1099
                     /gene="glmM"
                     /locus_tag="VK031_02020"
     CDS             <1..>1099
                     /gene="glmM"
                     /locus_tag="VK031_02020"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HLS52719.1"
                     /translation="VLLGIIPTPAVAYLTRKYKALGGVVISASHNPGEYNGIKFFNNE
                     GLKLSDEAENKIEESIKNIDKIDIRPVGEKLGKKYKEENGAKDYKDFLKSAIDIDLTG
                     MKIVMDCGHGALYKIGPEIIRELNGQVIVVNDKPNGMNINDNCGSTNPQLTQNLVVEE
                     NADIGISFDGDGDRIIAIDDKGNIVDGDSILAICASHLKEDSKLPKDTIVGTIMTNMG
                     LDIYLKERGMEIVKTDVGDRYVLEEMLSSGYVLGGEQSGHIIFLEHNTTGDGLATGLH
                     LLEVMKSTGKPLSQLNSLMKAYPQVLENAQVKNELKYKYLENQEIKEEIDRIEKLFEG
                     KGRVVIRPSGTEPLVRVMIEGEDQEKISQIAK"
BASE COUNT          438 a          131 c          246 g          284 t
ORIGIN      
        1 ggtgctttta ggaattatcc caactccagc agttgcatat ttaacaagaa aatataaggc
       61 tttaggcggt gtggttattt ctgcttctca taatccaggg gaatataatg gaattaaatt
      121 ctttaataat gaaggattaa aattatcaga tgaggcagaa aataagatag aagaaagtat
      181 aaaaaatata gataagatag atataaggcc tgttggtgaa aaattaggga aaaaatataa
      241 agaagaaaat ggggctaaag actataagga ctttttaaaa tcagccattg atatagatct
      301 aactggaatg aagatagtta tggattgtgg tcatggagcc ttatataaaa taggaccaga
      361 gataattagg gaattaaatg gccaagtaat agtggtcaat gataaaccaa atggtatgaa
      421 tattaacgat aattgtggct ccactaaccc tcaactaacc caaaacctag tggtggaaga
      481 aaatgcagat ataggtatct cctttgatgg agatggagat aggataatag ccatagatga
      541 taagggaaat atagtggatg gagatagtat actagccata tgtgccagcc atcttaaaga
      601 agatagcaag ttgccaaaag atacaatagt tggtacaata atgacaaata tggggctaga
      661 tatatatttg aaagaaagag gaatggaaat tgtaaaaaca gatgtaggcg atagatatgt
      721 attagaggag atgttaagtt caggatacgt attaggtggg gaacaatctg gccatataat
      781 ctttctagaa cacaatacta caggtgatgg cttagccaca ggcctacact tattggaagt
      841 aatgaaatca actggtaaac ctttatctca actaaactct ctaatgaagg cttatccaca
      901 ggtcttggaa aatgctcagg taaaaaatga gttaaaatat aaatatctgg aaaatcaaga
      961 aataaaagaa gaaattgata ggatagagaa actgtttgag ggcaaaggta gggtagttat
     1021 aagaccttct gggacagaac ctttagttag ggtgatgata gaaggagaag accaggaaaa
     1081 aattagtcaa atagccaaa
//
LOCUS       DATGSI010000130         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_244013, whole genome shotgun sequence.
ACCESSION   DATGSI010000130 DATGSI010000000
VERSION     DATGSI010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244013"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /locus_tag="VK031_02025"
     CDS             <1..1778
                     /locus_tag="VK031_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS52720.1"
                     /translation="KAVLFMESFYRLKDFFKDNKWSYLFGLFWLFLVDSVQLLVPRIL
                     GNLTNDLQNNILNMEGIIKYSLYIIITGLIIGFGRYFWRIYIIGNSRKLEYYLRKELF
                     DHLLNLSPNFFNTHKTGDLMSHATNDINAIRMALGFGVIMMIDSLFIIILALFMMVRI
                     TNLKLTAIAVFNLPIIVILTRRFGALIYNRSRDAQNALANLTEITQESFAGVRIIKSF
                     VQEEEVSNNFYQVNQNNFQKNLDLVKVSGSFRPLLQFVFSISLLITIFYAGKEVVYGS
                     ISLGSFIAFTSYLGLLNWPTRALGQVINVLQRGTASMDRINILLDEEPEITDSPTLVD
                     IEKVEGKIEFENVSFQYPNSNHKALKNINFTLKQGETLAIIGRTGSGKTTIANLLLRL
                     YNLQEGQIYIDDIEIEKIPLKTLRENIGYVPQDNFLFSDTLHNNIAFAFDQGVSKDRV
                     YKAAKMAEIYDNIISFPEQFETSLGERGVTLSGGQRQRTSIARALIKDSPILILDDSL
                     SAVDTETEENILNNLRSLTKNTTTIIISHRISTIQGADKILFLDEGRIVERGNHEELL
                     ALNGLYKDLYEKQLLEEKIKEKEDF"
     gene            1778..>2936
                     /locus_tag="VK031_02030"
     CDS             1778..>2936
                     /locus_tag="VK031_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS52721.1"
                     /translation="MSTPNEEINSRERVYDIRLFKRLLGYALPYWKFLLLSLILLIVI
                     TGLELLNPYLLKISIDEYINGNQKPIYQIDIDSPIEGIEFNNYKYVRKDNLSKEEKLK
                     LKDQPVKKIVKRNDVPYLVDFYNEDEKVALDQDTYSRFREEDIQGINRVSFYFFITIL
                     FTFLFNYLQIYLLNYTGQRIIFNIREEVFNHILNLSVAYFDKNPIGRLVTRVTNDTEA
                     LNEMYTSVLVNLFKDVFTLVGIIVIMLKMDHKLALLSFLLLPLILTVAIIFRRKIRVV
                     YRLARAQLAMINTNLNEYISGMRTIQIFKKEKKISNKFDYINQKYLDTSRREVQIYAT
                     LRPSIEIIRSLGIAALIYFGGGKVISSRMEFGMLYIFVDYLQRFFNPILDLT"
BASE COUNT         1002 a          444 c          508 g          982 t
ORIGIN      
        1 gaaaggcggt attatttatg gaatccttct acagattaaa agattttttt aaagataata
       61 aatggtccta tctatttggt ttattttggc tttttctagt tgactcggtc caattattag
      121 tcccaaggat tttaggtaac cttacaaatg atttgcaaaa taatatatta aacatggaag
      181 gaattataaa atatagtctt tatataatca taactggttt gattataggt ttcggtagat
      241 atttttggcg gatttatata attggaaact ctagaaagct tgaatattac ttaagaaagg
      301 aactttttga tcacctgtta aatctgtctc ctaacttttt caatacccat aaaactggtg
      361 atttaatgtc tcatgctacc aatgatatta atgctattag aatggcccta ggttttggtg
      421 ttattatgat gatagattct ctatttatta ttattttagc cttatttatg atggtaagga
      481 tcactaacct aaagctaact gccattgctg tttttaacct gcccataata gttattttaa
      541 ctagaaggtt tggagccctt atatataata ggtctaggga tgcccaaaat gcattggcaa
      601 atttaactga gattacacaa gagagttttg ccggtgtgag gattattaag tcctttgttc
      661 aggaagaaga agtatcaaat aacttttacc aggttaatca aaataatttt caaaagaatt
      721 tagacctggt caaggtatct ggttcctttc gccccctgct acaatttgtt ttttctataa
      781 gcttattaat tacaatcttt tatgcaggta aagaggttgt atatggaagt atttcattag
      841 gaagttttat agccttcacc tcctatttag gccttttaaa ttggcctact agggccttag
      901 gtcaggtgat taacgtttta caaaggggaa cagcctctat ggatagaata aatattttac
      961 tagatgaaga accagaaatt actgattcac ctactcttgt tgatatagaa aaagttgaag
     1021 gaaaaattga atttgaaaat gtgtctttcc aataccctaa tagtaatcac aaggccttaa
     1081 agaatataaa tttcacctta aaacaaggag aaaccctggc cattataggc cgtacaggta
     1141 gtgggaaaac taccatagcc aacctgttac ttagattgta taacctacag gaaggccaga
     1201 tctatatcga tgatatagag attgaaaaga ttcctttgaa aactttaagg gaaaatattg
     1261 gctatgttcc tcaagataat tttcttttct ctgatactct gcataataat atagccttcg
     1321 cctttgatca aggtgtttca aaagataggg tctacaaggc agcaaaaatg gctgaaatat
     1381 atgataatat catatccttc ccagaacaat ttgaaacaag tttaggagaa aggggagtca
     1441 ccctttccgg cggccaaaga caaagaacat ccattgccag ggccctgata aaagattctc
     1501 ctattttgat tttagatgat agtttgtctg ctgttgatac agaaactgaa gaaaatattt
     1561 taaacaattt aagatcactg actaaaaata ctactactat tattattagt cataggattt
     1621 ccaccatcca aggggcagat aagattcttt tcttagatga gggaagaatt gtagaaagag
     1681 gtaaccatga agaattacta gcccttaatg gcctatataa ggatttatat gaaaaacaat
     1741 tgttagagga aaaaattaaa gaaaaggagg acttttaatg agcacaccta atgaagaaat
     1801 taattccaga gaaagagtat acgatattag attgtttaaa agattactag gctatgcctt
     1861 gccttactgg aaattcctcc tattatcttt aattttactg attgttatta cgggtttaga
     1921 actacttaat ccttacctgc tgaagatttc catagatgag tatataaacg gtaaccaaaa
     1981 gcccatttac caaatagata tagattctcc tattgaaggc atagagttta acaactataa
     2041 atatgttcga aaagataatt taagcaaaga ggaaaagtta aaactaaagg accagccggt
     2101 taaaaaaatt gtaaaaagaa atgatgtccc atatttagta gacttttata atgaggatga
     2161 gaaagtagca cttgaccagg atacctattc tagatttaga gaagaagaca ttcaaggtat
     2221 aaatcgggtt tctttttatt tcttcataac catccttttt acctttttat ttaattattt
     2281 acaaatttat ttattgaact atacgggcca aagaatcatt ttcaatattc gggaggaagt
     2341 ctttaatcac atactaaatc tgtcagtggc ctatttcgat aaaaatccaa ttggtaggct
     2401 ggttactagg gttaccaatg atacagaagc cttaaatgaa atgtatacct ctgttttggt
     2461 aaatctgttt aaagatgtct ttaccttggt gggcataatt gtaattatgc ttaaaatgga
     2521 tcataaatta gccttattat cctttcttct tctgccctta atcctaactg ttgccattat
     2581 atttagaagg aaaattaggg tagtttacag attggccaga gctcaactgg ccatgattaa
     2641 cacaaatcta aatgaatata tttctggcat gagaactatt caaatcttta aaaaggagaa
     2701 aaaaatatct aataaattcg attatattaa tcaaaaatat ttggatacca gcagaagaga
     2761 ggttcaaatc tatgccactc ttagaccttc catagaaata atcagatcct taggtattgc
     2821 cgccctcata tattttggcg gtggtaaggt tataagtagc aggatggaat ttggtatgct
     2881 ttacattttt gttgattatt tacaaaggtt tttcaatcct attttagatt taaccg
//
LOCUS       DATGSI010000131         1569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_200624, whole genome shotgun sequence.
ACCESSION   DATGSI010000131 DATGSI010000000
VERSION     DATGSI010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200624"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VK031_02035"
     CDS             <1..210
                     /locus_tag="VK031_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HLS52722.1"
                     /translation="TIGTSQEGADKQTIRNIALYSKMAGADIHHLGDAGMTVGMATPE
                     NIMAYSIAIRGKRHTYRRMARSVNR"
     gene            225..704
                     /locus_tag="VK031_02040"
     CDS             225..704
                     /locus_tag="VK031_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HLS52723.1"
                     /translation="MLKLFSKRKKHKLLAPITGEIIELSQVPDEVFANKMVGDGIAIR
                     PTENEVLSPCDGKVVQLFPTNHAIGIETKEGIEVLIHIGIDTVSLKGNGFKSFVTQGD
                     HVRTGDKLIEVDLAYIEKNATSTITPIIITNMDKIKEFKNFNGYVKKGIDKIMELKL"
     gene            911..>1569
                     /locus_tag="VK031_02045"
     CDS             911..>1569
                     /locus_tag="VK031_02045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HLS52724.1"
                     /translation="MINFVHTGDLHLGLQFRQVSFDKKRAKERRRELWTSFQRIVDYC
                     VEKKKDFLFIAGDLFEHKYFTLGDIKRVRDILAGAAEVNVVIAAGNHDYLNENSLYNQ
                     VDWTCNVHIFGSTGLEKKVYDDLNTVVYGYSWNRMEIGENSLFDDFRPVDVDYKKILL
                     IHGDVGYKSNYLPLDLNMLKSMDMDYIALGHIHKPEIFTEKIAYCGCPEPLNFGETGD
                     RG"
BASE COUNT          578 a          194 c          346 g          451 t
ORIGIN      
        1 accataggga catctcagga aggggcagat aaacaaacaa taagaaatat tgccctatat
       61 tcaaagatgg caggtgctga tatacatcat ttaggggatg ctggaatgac agttggtatg
      121 gctaccccag aaaatataat ggcctattcc attgctataa gaggtaagag acatacctat
      181 agaaggatgg ctaggtcagt aaatagatag gagaaatgca acaaatgtta aaattattca
      241 gcaaacgcaa aaaacacaaa ctattggcac caataactgg agagattata gaattatcac
      301 aagttcctga tgaagtattt gccaataaaa tggtaggtga tggtatagca ataagaccaa
      361 cagaaaatga ggtactatca ccctgtgatg gaaaggttgt tcagctattt ccaacaaatc
      421 atgcaattgg aatagaaacc aaggagggaa tagaagtatt aattcatata ggaattgata
      481 cagtaagctt aaaagggaat gggtttaaaa gttttgttac ccagggggat catgtacgaa
      541 ctggtgataa actaatagag gtagacttgg cttatataga aaaaaacgcc acgtctacca
      601 taacccctat aataatcacc aatatggata aaataaaaga atttaaaaat tttaatgggt
      661 atgtaaaaaa gggaatagat aagattatgg aacttaagct gtaagttatt agtaatatgc
      721 tgtattcgat ttttaaatta agggttccaa ttggcaggta tactgccgca atgtataaat
      781 ggggaaatat ctcttttccg gtaagaaaca acttactgga aaggagattt ttattatatt
      841 gaaccttaat actggcagtt gagatttacc ccttacttta tggtataatt ttaaaagggg
      901 gtggaataaa ttgataaatt ttgtacatac aggagattta catttaggcc tacagtttag
      961 acaggtatcc tttgataaaa aaagagctaa agagcgaaga agagagttat ggacaagttt
     1021 ccaaaggatt gttgattatt gtgttgagaa gaagaaagac ttcttattta ttgcaggaga
     1081 tctatttgaa cataaatatt ttaccttagg ggatataaaa agagtcagag atatattagc
     1141 aggggcagca gaggtaaatg tggtgatagc tgctggaaat catgattatt taaatgaaaa
     1201 ctccttatat aatcaggtgg attggacttg taatgtgcat atatttggta gtactggttt
     1261 agaaaagaag gtatatgatg acttaaatac tgtagtgtat ggctattcct ggaataggat
     1321 ggagataggg gaaaatagct tgtttgatga ttttaggccg gtagatgtgg attacaaaaa
     1381 aatactgctt attcatgggg atgtaggata taaatccaac tatttgccat tagacttaaa
     1441 tatgcttaaa agtatggata tggattatat agccttggga catatccata aaccagaaat
     1501 atttacagaa aagattgcct attgtggttg tcctgagccc ttgaattttg gagaaacagg
     1561 agacagagg
//
LOCUS       DATGSI010000132         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_339117, whole genome shotgun sequence.
ACCESSION   DATGSI010000132 DATGSI010000000
VERSION     DATGSI010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339117"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1900)
                     /locus_tag="VK031_02050"
     CDS             complement(230..1900)
                     /locus_tag="VK031_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS52725.1"
                     /translation="MFFAFLVSVFDLVFPIFTRTMINDIIPNGRMDLLLRWSMIMMFL
                     FCIRYISNYIVSYWGHVLGVRIEHDMRSDIFKHLQTLSFSYFDNTKTGHIMSRIVNDL
                     RDITELAHHGPEDLFISFVMLLGSFIILMTIEWRLTLIIFASVPVMIIFAIRKRKKME
                     ESFKEVRKKIADVNSQLENSISGIRVAKSFTNESYEIEKFEQGNLAFSEARQQSYKIM
                     AEFVSGIGIISNVLNLLVISVGGLFVYQNVITVADLFAYTLYVNFFMQPIRRLTDFTQ
                     QLQDGMTGFERFLEVMKIEPDIVDREDAIEFEQVKGRIDFKDVSFSYNNGEDRVLEDI
                     NLSIEEGKTVALVGPSGAGKTTLCHLIPRFYEIDKGSILIDNKDIRDIKLSSLRSAIG
                     MVQQDVFLFTGTIRENILYGNPDANEDEIIEAAKKASIHDFIMSLPEGYDTYIGEKGV
                     MLSGGQKQRISIARLFLKNPPILILDEATSALDNETEVMIQASLEELAKGRTTLVIAH
                     RLSTIKNADEIIVLTEEGIVEQGNHDRLMAKKGIYYSLYNSQFHKLAV"
     gene            complement(2127..3023)
                     /locus_tag="VK031_02055"
     CDS             complement(2127..3023)
                     /locus_tag="VK031_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLS52726.1"
                     /translation="MKKTIGIDLGGTSILAGVIDKRGNILKQARRDSSKAQGREEVLD
                     HMADLINELLDEDIIGVGIGSPGFIDSDQGKVLKIGGNIKGWANTDVKREILLRIPKL
                     PLFIENDANVAALCEGWIGAAKNFKNFLMLTLGTGVGGAIFTEETKILHGHRYQGAEL
                     GHMIMYPKGRACGCGQRGCVEQYISGSAVERRYKELSGQGKKGKDIFKDSKEDKWAGQ
                     TVDEFAEHLSISLVSLKNIFDPEAIIIGGGVINSKKYWWDLVLDYYKQHSNDPEGMEI
                     LPASYLNDAGMIGAGRLVFDKV"
     gene            complement(3107..>3475)
                     /locus_tag="VK031_02060"
     CDS             complement(3107..>3475)
                     /locus_tag="VK031_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS52727.1"
                     /translation="RAQVLNLLTKLQKRKGLTYLFIAHDLSVVRYITDRVAIIYKGKI
                     VELAETEELFKNPLHPYTRALISAIPLPDPRLEKNKKITLYDPSVHNYKLDPPKWVEI
                     RPNHFILANSEEVKRYEKLI"
BASE COUNT         1046 a          741 c          484 g         1204 t
ORIGIN      
        1 tgcccccaac tatcattata gcaagactta aaagtagcag ggacatgata taccagccct
       61 tttttctttt cattatgttc accccatagt aatgttgtca ttactataat agtattatac
      121 aatcaaattt tgatagttac aatcatatta atattaagtt acaattagtt tacagtttgt
      181 ccaaaaaaac aataggctgt agcctattgt ttaaacatta gatattttct tataccgcca
      241 gtttatgaaa ttgagaatta tacaggctat aataaatgcc tttcttagcc attagcctgt
      301 catggttgcc ctgttctaca atcccttcct cagtcaaaac tataatctca tctgcatttt
      361 ttatagtaga cagcctatgg gcaataacca atgtggtcct acccttggcc aattcctcca
      421 gggaggcttg gatcataact tctgtttcgt tatctaaagc agacgttgcc tcatctagta
      481 ttaagatagg aggatttttt aaaaacagcc tggctataga aatcctttgt ttttgtcctc
      541 cagataacat aactcccttc tctccaatat aggtatcgta tccttctggc agggacatta
      601 taaagtcatg aatacttgcc tttttagccg cctcaataat ttcatcttca ttggcatctg
      661 gattgccata caaaatattt tccctaatgg ttccagtaaa caagaataca tcctgttgaa
      721 ccatacctat ggcacttctt aaggagctta attttatatc cctaatatcc ttattatcta
      781 taagtatgct tcccttatct atttcataaa atctaggaat taaatggcac aaggttgtct
      841 ttccagcacc tgagggtccc actagggcaa cagtttttcc ttcttcaata cttagattaa
      901 tatcctcaag aaccctatct tctccattat tatagctaaa ggatacatct ttaaagtcga
      961 tccttccttt tacctgctca aactctatgg catcttccct atctacaata tcaggctcaa
     1021 tcttcataac ttccaagaat ctttcaaagc cagtcatgcc atcctgtaat tgctgggtaa
     1081 aatcagttag ccttctaata ggttgcataa agaaatttac ataaagggtg taggcaaata
     1141 ggtcggctac agttattaca ttttgataga caaataaccc acctacagat ataactagca
     1201 gatttaatac attagatata attccgatac ctgatacaaa ctctgccatt attttgtaag
     1261 attgttgcct agcttcggaa aatgccaagt tcccctgttc aaacttttca atttcataac
     1321 tttcattggt gaaggacttt gccactctta tacctgatat actattttcc aattgggagt
     1381 tgacgtctgc tatctttttt ctcacttctt taaaagattc ttccatcttc tttctttttc
     1441 ttatggcaaa tataatcata actggtacag aggcaaatat tatcaaggtt agcctccatt
     1501 caatagtcat tagaattata aaggaaccaa gtaacataac aaaggatata aacaaatctt
     1561 caggtccatg atgggccaat tctgtaatat ctcttaaatc attgacaatc ctagacataa
     1621 tgtgaccagt ttttgtatta tcaaaataac taaaagataa agtttgtaaa tgtttgaaga
     1681 tatcacttct catatcatgc tcaattctaa cacctaggac atgtccccag taagatacaa
     1741 tataattgct aatgtatctt atacagaata aaaacatcat tatcatgctc catcgtagta
     1801 aaagatccat tcggccatta ggtataatgt cattgatcat ggtccttgta aaaattggaa
     1861 ataccaaatc aaatactgaa actaagaatg caaaaaacat atctataaaa aataatcttt
     1921 tatgtggttt ataatatgat ataaattctt taagcaataa ttccactcct ttagtttaca
     1981 aatcaataaa taaatctttc gacacacgaa tattatatat gttttggttg gtcttttcaa
     2041 tattaattaa tatttatata cccttcttaa gtaagcttag taaatcttga tgcaatttaa
     2101 aaaagcccca aaagggactt ttatctttaa actttgtcaa aaaccagccg tcctgcacct
     2161 atcattcctg catcatttaa atagctggct ggtaaaattt ccattccttc cggatcattg
     2221 gaatgttgtt tataatagtc caacaccaga tcccaccagt atttcttaga gttaattact
     2281 cctccaccta ttatgatggc ttcaggatca aaaatatttt ttaaacttac caagcttatg
     2341 gataaatgtt cggcaaattc atccactgtc tgtccagccc atttatcctc ctttgaatct
     2401 ttaaatatat ctttgccttt tttgccttgg cctgataatt ccttatatcg cctttctacg
     2461 gcactaccag agatatattg ctccacacag cctctttgtc cacaaccaca ggctctaccc
     2521 tttggataca taatcatatg acctaattct gctccctggt atctatggcc atgtaaaatt
     2581 ttagtctctt cagtgaatat ggcaccaccg acgccagttc ccagagtgag cattaaaaag
     2641 tttttaaaat tcttggcagc acctatccac ccctcacaga gagctgccac atttgcatca
     2701 ttttctataa ataatggaag tttaggaatc cttaataata tttctctctt tacgtctgta
     2761 ttggcccatc ccttaatatt accacctatt tttagcacct tgccctggtc tgaatctata
     2821 aaccctgggg aaccaatacc tactccaatt atatcctcat ctaaaagttc gttgattaag
     2881 tcggccatat gatctagaac ttcttcccta ccttgagctt tagaactatc tcttctggcc
     2941 tgttttaaaa tattacctct cttatctatt accccagcca ggatactggt gcctcccagg
     3001 tctattccta ttgttttttt catcccttcc acctctttcc ttatccttta ttttattata
     3061 ttattttccc cttgacaact aatataagga attgtcctag taaattttaa attagttttt
     3121 catacctttt aacctcttca gaattggcta agatgaagtg attgggacgt atttccaccc
     3181 atttaggtgg atcaagctta taattgtgga cactagggtc atatagggta atttttttgt
     3241 ttttctccag cctaggatcc ggcaggggaa tggcagaaat taaggctctt gtatagggat
     3301 gtaatgggtt tttaaatagt tcctccgtct cagccagctc cactattttc cccttatata
     3361 ttatggccac cctatctgta atatagcgaa ccactgacaa atcatgggct ataaataagt
     3421 aagtcaaacc cttccttttt tgtagcttag taagcaaatt taagacctgg gccct
//
LOCUS       DATGSI010000133         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_577592, whole genome shotgun sequence.
ACCESSION   DATGSI010000133 DATGSI010000000
VERSION     DATGSI010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577592"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..782)
                     /locus_tag="VK031_02065"
     CDS             complement(279..782)
                     /locus_tag="VK031_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52728.1"
                     /translation="MITNGVLDIVELWKSTVERLNQQNSCSCCWEFYAPLTEVKLNIV
                     RNKGNCCTHVFLLRNKPNDFTSALTYNNGYLNTEVNTENYDVLFLISSKTGLNNYNEL
                     AHHPIEESRYATIFKPLRECIKSDIITALCEHAQVTSWSGRYVYDYQDENYYGLRISI
                     SQRYERH"
     gene            complement(772..1230)
                     /locus_tag="VK031_02070"
     CDS             complement(772..1230)
                     /locus_tag="VK031_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52729.1"
                     /translation="MIWKILDENIERSNKLGKWISPDVIDYTYWEVRYILPDKLSDIT
                     ELINYICDKHKINKDKLSQAEIISFFLFMKDSLVRIAEVEQGNLQPRVVNKSKFKPSP
                     RAKDYPAIIELNRLSGGCPIKAKEIKEMKYSEVFETLLALTIESEGTYDN"
     gene            complement(1227..2549)
                     /locus_tag="VK031_02075"
     CDS             complement(1227..2549)
                     /locus_tag="VK031_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HLS52730.1"
                     /translation="MERQGREQEQYQPKSRPTNIRLKDKRQVMPEAKFITTTASIKLS
                     KMREKYRIIPGGTSAGKTFNIIPIIYSRALLMPNKVFSITSETMPHLRKGAMRDFKNI
                     LRMTGRWDRSKWHGTDSIYSLDNGSVIEFFSLDDESKARGPRRDELYVNEADAIKFEI
                     FRQLQIRTRGVVWIDFNPHTEFWVHENLKDHPRAEWLTLTYKDNEALDESLVKEIEHA
                     LELGFHDPTLIGDDLFQEENIKNKYWSNWWKVYGLGQLGEIDELILRDWEQVDSIPIN
                     AKYIGSGLDFGFNPDPSTLVDMYLYNGQKYFKEIIYERNLGVAELANKIKQDGLTRNI
                     VADRSAPLIISDLNTYGLNVVAYDATDGMNTIESGISILRSDKFHVTSDSLNLIKELR
                     RYIRIDGRIPNNAEDHAIDAMRYIGSTKRNTRSPVRSRGRRPQRQRLR"
     gene            complement(2465..2851)
                     /locus_tag="VK031_02080"
     CDS             complement(2465..2851)
                     /locus_tag="VK031_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52731.1"
                     /translation="MVGRPKKNLDILPEEWYLHVLELYSEGASDVEIKAYIYNELGTF
                     SNDLWDRWLKEEPEFSETIKKGRMLSEAWWSRSGRTNLENKDFNYTGWYMNMKNRFGW
                     SDRVESKSNTNLKVDQPIFDLKINDK"
BASE COUNT          976 a          710 c          539 g         1361 t
ORIGIN      
        1 cctgatgcgt tatgccctcg ctcttcaaaa tctggaataa caaatttgtt aataacctct
       61 aatgttatct gtcttatttc ctcttctgat atcacttcct ccatgatttg tatagtatta
      121 cagtaaagga tataataaaa gtcattagtg ctagtgtacc tacagcaata acagccgaaa
      181 acacatataa ccaagttaac ccaaagatta gcttaattat aagattaaat atagtgatat
      241 agcttactat tgcaatcatc cattgccata cgtttaattt agtgcctctc atatctttgg
      301 cttattgaaa ttcgtaatcc gtaataattt tcatcttgat agtcgtatac gtaacgtcca
      361 ctccagcttg ttacctgtgc atgttcgcat agtgctgtta taatgtctga ctttatacat
      421 tctcttaatg gtttaaatat agtagcgtat ctactctctt ctattgggtg atgcgctaac
      481 tcattgtaat tatttaatcc agtcttagat gagattagaa ataatacatc gtagttttcg
      541 gtgtttactt ctgtattgag atatccatta ttatacgtta atgcgcttgt aaagtcgtta
      601 ggtttattcc taagtaagaa tacatgagta cagcaattgc ctttattcct aacaatattt
      661 agcttaactt ctgttagtgg tgcgtagaat tcccagcagc aactacatga gttctgctga
      721 ttaagtcttt ctactgtaga cttccatagt tcgactatat ctaatactcc gttagttatc
      781 ataagtcccc tcgctttcaa ttgttaacgc caataaggtt tcaaatactt cgctatattt
      841 catttctttt atttcctttg cctttattgg acacccgccg cttaacctgt taagctctat
      901 tattgcagga taatctttag ctcttggtga tggtttaaat ttactcttat ttaccactct
      961 tggctgtaag ttaccttgct ctacttctgc aatcctaact aagctgtctt tcataaatag
     1021 aaagaagcta attatctctg cttggcttaa cttatcctta ttgattttat gtttatcaca
     1081 aatataatta attaattctg ttatatcgct aagcttatca ggtaatatat acctaacttc
     1141 ccaatatgta tagtctatta catctggact tatccacttt cctagcttat tagatcgctc
     1201 tatattttcg tcaagtatct tccaaatcat ctgagtcgct gtctttgtgg tcgccttcca
     1261 cgtgagcgaa ctggtgacct agtgtttctt ttagttgaac caatataacg catagcatct
     1321 atagcatggt cttctgcatt gtttggtata cgcccatcta ttcgtatgta acgccttagt
     1381 tctttaatta agttaaggga atcgcttgta acatggaatt tatcacttct taaaatagag
     1441 ataccacttt ctattgtgtt catgccatca gtagcatcat aagctactac atttaatcca
     1501 taggtattca agtccgatat aattaaaggt gcgcttctat ctgctactat atttcttgtt
     1561 aatccgtcct gcttaatctt attagctaat tctgcaactc ctaaattacg ttcatatatt
     1621 atttctttga agtatttttg cccattgtat aaatacatat ccaccaatgt ggatgggtca
     1681 gggttgaatc caaagtctaa tcctgaaccg atgtacttgg cgttgattgg tatcgaatct
     1741 acttgctccc aatctctaag tataagctcg tctatctctc caagctgtcc aagtccatat
     1801 accttccacc aattactcca gtatttattc tttatatttt cctcttgaaa taagtcatca
     1861 ccgattaatg tggggtcatg aaatcctaac tctaaagcat gttctatttc cttgactagt
     1921 gattcgtcta gcgcttcatt atccttatag gttaacgtca gccattcggc tctaggatgg
     1981 tcttttagat tctcatgcac ccaaaactct gtatgtgggt taaagtcaat ccaaactaca
     2041 ccccttgttc taatctgtaa ttgcctgaat atttcaaact taattgcatc ggcttcgtta
     2101 acgtatagtt catcacgcct tggtcctctt gctttacttt cgtcgtctag agaaaagaat
     2161 tcaataacac taccattgtc tagcgaataa atagaatcag taccgtgcca tttagatctg
     2221 tcccatcttc cagtcatgcg aagtatattc ttaaaatctc gcattgcacc ttttcttaag
     2281 tgtggcattg tctcagatgt tatagagaat accttattcg gcattagcaa agctcttgaa
     2341 taaataatag gtataatatt gaaagtcttt ccagcagaag taccccctgg tattatcctg
     2401 tatttctcac gcatctttga tagctttata gatgctgtag ttgttataaa ctttgcctct
     2461 ggcattactt gtcgtttatc tttaagtcga atattggttg gtctactttt aggttggtat
     2521 tgctcttgct ctctaccctg tcgctccatc caaagcgatt cttcatattc atataccatc
     2581 cagtataatt gaagtcttta ttctctaggt tagtacgtcc tgacctactc caccaagcct
     2641 cagaaagcat cctacctttt tttatggttt ccgaaaattc tggctcttcc tttagccatc
     2701 tatcccataa gtcattagag aatgttccaa gttcgttata aatatatgct ttaatctcaa
     2761 catcagaagc accctcagaa tacaattcta atacgtgtaa gtaccattcc tctggtaata
     2821 tatctaagtt ctttttaggt cttcctacca tattattata ttattagcct tatagcaaat
     2881 ataagtaata taaacaataa aagccactat tttatgtagt ggctttggtt tagggttaaa
     2941 ttaggggttt aatcaatctt ctcacatttg ataatgtaat aatcttctct ttgctttaga
     3001 tatctatcta tatatttttg agtgttacag atagtgtatc tatttgtctt tgggctgtat
     3061 ataaattctt tctctataca ctcccagcaa tatgtaactt catcagatct tatacctgag
     3121 ggactatcat ctggactaca agcgaatgtt aatagtccga ttgttaaaat tgttagtgtc
     3181 tttttcatat ctataagttt ttaattgttc ctgttgatga aacgtttggt ttacccagtt
     3241 tcgattctaa ttgtttaatc ctttctaaag cttctaggct tagagcgtct cctctaaact
     3301 tcagcttgtt ttttagtgtt gtgttgttca tggtttgtca ggtttaagaa ttgataatat
     3361 aatctaattg ttttttcagc ttagaatgaa gttctttaaa gtaaatatgt tcctcgctat
     3421 ttacttcggt aaatttaact tttccagtta ctaaaaacat taatgttatt aagtcgttaa
     3481 ttgtcatatc catatctatt ttattttaaa gttagttgca agggcaggac tcgaacctgc
     3541 atgacggctt tcaatctacg ggcagtagcc taactaactt cccctt
//
LOCUS       DATGSI010000134         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_577616, whole genome shotgun sequence.
ACCESSION   DATGSI010000134 DATGSI010000000
VERSION     DATGSI010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577616"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VK031_02085"
     CDS             <1..333
                     /locus_tag="VK031_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HLS52732.1"
                     /translation="TLRASEKVDWTGWKDLKVQLPDNKALYPLKVKNIYIKLPKNSQF
                     EATLIFDNLHLYDDYFTYKVKAGDTIFDISLKFYGTSKYMNEILRLNNMNITDILPVG
                     KLLKLRVP"
     gene            348..797
                     /gene="comGD"
                     /locus_tag="VK031_02090"
     CDS             348..797
                     /gene="comGD"
                     /locus_tag="VK031_02090"
                     /inference="COORDINATES: protein motif:HMM:NF040982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HLS52733.1"
                     /translation="MKIDNRVAYTLMELLLVLAILSIISLLTIPNTKFFLNMREQQEL
                     NELKKDLLYARNRAIVEGRSYFVYFDRERNQYIIRYSETSPPVKVKEFKHGIKIWKDN
                     STSILRFNANGRPGESGSIYLVDRKNNRYKLSLTPATGRIELGILDK"
     gene            809..1192
                     /locus_tag="VK031_02095"
     CDS             809..1192
                     /locus_tag="VK031_02095"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLS52734.1"
                     /translation="MKRKAYSLVEILVGLFILGLITTTVMPIIHTSFNSLCRAGEKME
                     MLHLAEEVAENLRGKPENYQALLEELEEKEKVTFTDLSEKYREKYGCQLIDMGSSDKF
                     WKLKILVDKRKGSSYVELELKLPKT"
     gene            1158..1748
                     /locus_tag="VK031_02100"
     CDS             1158..1748
                     /locus_tag="VK031_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLS52735.1"
                     /translation="MWNLNLSFQRLKGFSLIELILWLALFSVLMTASISLLINVNGLI
                     NRGHNQDTMLLTGQYALDYIKKEILGADKIIPSSKITNLDRIYPNNIGFVILNIKAED
                     NEFTGKKIYTYNYSTYYLKNDQLIRIATNRNDPNYPPAKEFSGYNQLCKGVAKLWKTR
                     LDMVNKMIHLNLTISVANAQEQNFKSTIKLNCPIDY"
     gene            1763..>2301
                     /locus_tag="VK031_02105"
     CDS             1763..>2301
                     /locus_tag="VK031_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52736.1"
                     /translation="MKRSGSITVIVIYISFIIFLGAMSLVYYASLQALISNNLIDFSQ
                     SKYILEDKLYELIYDGQAFEKYFLGNIFTKFRGGFLPPDGIWKLNLEGSLKDHVTRAE
                     YSIEKIDEKMNLILQVEIRYKKVRRALKSYISLVNNFFENGESYIFKEDLSNNDEKIL
                     LKLFEVIQDELIDFNKEATG"
BASE COUNT          856 a          293 c          444 g          708 t
ORIGIN      
        1 actttaaggg ccagtgaaaa agtagattgg actggatgga aagatttaaa agttcagctg
       61 cctgataata aggccctata tcccttgaag gtgaaaaata tatatattaa attaccaaag
      121 aatagccagt ttgaagctac cttgattttt gataatctac atttgtatga tgattatttt
      181 acttacaagg ttaaagctgg tgatacaata tttgatatat ctctaaaatt ttatggaaca
      241 tctaagtata tgaatgaaat tcttagacta aacaatatga acataacaga tattcttcct
      301 gtaggaaagc tattaaaact aagagtacca taaaagagag gttaaggatg aaaatagata
      361 atagagttgc ttatacactg atggagttat tgttggtgtt ggccatatta tctattattt
      421 ctttattgac aattcctaat actaaattct tcttaaatat gagggagcaa caagagctta
      481 atgaattgaa aaaagactta ttgtatgcaa gaaatagggc catagtagag ggtagatcat
      541 attttgtata ctttgatagg gaacggaatc agtatattat caggtattca gaaacaagcc
      601 cacctgttaa ggtaaaagaa ttcaagcatg gaataaaaat atggaaagat aattcaacat
      661 cgattcttcg atttaatgca aatggtagac ctggagaaag tggcagcatt tatctagtgg
      721 accgtaaaaa taatagatat aaattaagcc taacaccagc aacaggcagg atagaattag
      781 gaatattaga taaatagggg aaatgcatat gaagagaaaa gcttatagtt tggttgaaat
      841 tttggttgga ttatttattt taggtttaat aactactact gtcatgccca taatccatac
      901 tagtttcaat agtttgtgcc gggcagggga aaaaatggaa atgctacacc tggcagaaga
      961 ggtggccgaa aacttaagag gaaaacctga aaattatcag gctttattgg aggagttgga
     1021 ggaaaaagaa aaagtgacct ttacagattt aagtgagaaa tacagggaaa aatatggctg
     1081 tcaacttatt gatatgggca gtagtgataa gttttggaag ctgaaaattc tagtagataa
     1141 aagaaagggt tctagttatg tggaacttga acttaagctt ccaaagactt aaaggtttta
     1201 gcctaattga actgattctt tggctagccc tgttttctgt gttgatgaca gccagcataa
     1261 gcctattaat caatgttaat ggactaataa ataggggtca caatcaagat actatgttac
     1321 ttactggtca atatgcccta gattatataa aaaaggaaat cctaggtgca gataagatca
     1381 taccatcttc taaaattact aacttagata ggatctatcc taacaatata ggttttgtga
     1441 ttttaaatat taaggcagaa gacaatgaat ttacaggtaa aaaaatttat acctataatt
     1501 actctaccta ctatttaaaa aatgatcagc taattaggat agctacaaac aggaatgatc
     1561 caaactatcc accggccaag gaatttagtg gatacaatca attatgtaag ggagtggcca
     1621 agctttggaa gactagatta gatatggtta ataaaatgat acaccttaat ttaaccatat
     1681 cagtagctaa tgcacaggaa caaaacttta agagtactat taaactaaat tgtcctatag
     1741 actattaact tgtggaggta atatgaaaag aagtgggtct attactgtaa tagtcattta
     1801 tataagtttt attattttcc taggagccat gtctttagtt tattatgcaa gtctacaggc
     1861 cttaattagc aataacttaa ttgatttttc ccagtcaaaa tatatactgg aggataagct
     1921 ttatgagcta atttatgacg gacaagcctt tgaaaaatat tttttaggca acatttttac
     1981 taaatttaga ggaggatttc tgcctccaga tggaatttgg aaactcaatc tggagggtag
     2041 cttaaaggac catgttacca gggcagaata tagcatagaa aaaattgatg aaaagatgaa
     2101 cctgatttta caggtggaaa tcaggtataa aaaagtccga agggctttaa agtcctatat
     2161 atccttagta aataattttt ttgaaaatgg agaatcctat atatttaagg aggatttatc
     2221 taataatgat gaaaaaatat tattaaagtt gtttgaagtc attcaagatg agttaataga
     2281 ttttaataag gaggctactg g
//
LOCUS       DATGSI010000135         6828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_200710, whole genome shotgun sequence.
ACCESSION   DATGSI010000135 DATGSI010000000
VERSION     DATGSI010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200710"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VK031_02110"
     CDS             <1..900
                     /locus_tag="VK031_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HLS52737.1"
                     /translation="GQMGERQLEQVMLDFIDKKQDVLVCTTIIETGLDIPNVNTIIVY
                     NADKMGLSQLYQLRGRVGRSDRIAYGYFTYEKDKVLTEVAERRLRAIKEFTEFGSGFK
                     IAMRDLEIRGAGNLLGVEQHGHIEAIGYDLYVKFLGEAIRRLKGEKVVEAIDTTIDIN
                     IDGYIPVNYIEDEELKIEVYKKIASISDLEEYRELIDELIDRFGDIPIEVQNLIDISY
                     IKALASRNQIKNISQTNNEIKLDLVSNDKLPVELIHLLSEKYGKAMSFDLSSNPYLKL
                     KSSKKLIVALRELVELIDDFHKN"
     gene            1049..1597
                     /gene="spoVT"
                     /locus_tag="VK031_02115"
     CDS             1049..1597
                     /gene="spoVT"
                     /locus_tag="VK031_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HLS52738.1"
                     /translation="MKATGIVRRIDDLGRVVVPKEIRRTLRIREGDPLEIFTDREGQV
                     ILKKYSPIGELNEFAGEYCESLYDNTGHTAIISDRDNIIAISGGSRKEYLEKRISPDL
                     EKIVESRSTFTTNDNSPIKIFYEDESSDDYVAQVITPIVMQGDPIGSIILLSKEAGTK
                     MGDLEVKLLETAAGFLSKQMEH"
     gene            1749..3368
                     /locus_tag="VK031_02120"
     CDS             1749..3368
                     /locus_tag="VK031_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HLS52739.1"
                     /translation="MILLSKNNSFIKGAAILSIAGIIVKILGAIYRIPLANIIKDEGM
                     GYYQTAYPFYVLLLTISTSGFPVAIAKLVSEKRALGDHRSAYRVFKVALTGLTIGGLL
                     SSLFVLFMAEDIVRSIDNINAYYSLVALVPALFFVPIMAAFRGFFQGRKNMVPTALSQ
                     IGEQFLRVVVGLSLTYYLLDRGIPVAAGGASFGGSAGAILGTVIIFIIFLLHKESIER
                     EVRQTRIQTDYSVGEIIKDLLTIAVPITIGAAIIPTMDTIDAGLVIRRLQSIGYSKET
                     ANELYGQLKGFAQTLINLPQVFSVAIGMSLVPAIADANARRNKREIRQIIGSGIRMTL
                     LIGLPCAFGLFVLAKPIINLLYFKNTIETINSSGEILAYLSFGVIFLTLVQALTAMLQ
                     GLGKPLIPVINLFIGALLKIILTYILTAIPGINIKGAAISTVVAYATAAILDLILLSK
                     VAKIKFNYRDIFIKPLISALGMAIIVRFSYGFLLKIIGGRLSTILSIGIGGLAYVILL
                     IVTGTITEEDIKFIPKGERLGRYLDKFKLLK"
     gene            3393..4844
                     /gene="mazG"
                     /locus_tag="VK031_02125"
     CDS             3393..4844
                     /gene="mazG"
                     /locus_tag="VK031_02125"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HLS52740.1"
                     /translation="MGKIYIIGLGPGSVDHLTLGAIERIHSGHKNFLRTEKHPTVSYF
                     LKNNIDFESFDYLYDKEEDFEGLYNNIADSLIKEARERKVINYFVPGNPLVAEKTVEI
                     LLQKDIETEIISGMSFIEPMVEIVGRDPINGLKIVDGSDFDSLMIDINVDTIITQVYN
                     RRIMSDIKLILAEIYGDEYNIWVINGAGIEEEEKVHNIPIYNLDRIDKIGYLTSVYIP
                     SVNNEREGIYDFNNIVKILRILRGENGCPWDIKQDHKSIRNCVIEEAYEVVDAIDSGD
                     IDNFIEELGDLLLQVIFHGEIANDEGEFNLIDITSKLAEKLIYRHPHIFLVKEVENTQ
                     EVGYNWNELKDSQRGLSSVVEKMTSLPALPALMKSYKIQDRAAQVGFDWDDIRGPLDK
                     IMEEYEEVLEAIETKDKDKIEEELGDLLFAVVNLARFLKVDPEVALNGTNNKFIKRFA
                     IMEKEATKAGKKLEHMSLSQMDLLWDKAKEDKS"
     gene            4915..5193
                     /locus_tag="VK031_02130"
     CDS             4915..5193
                     /locus_tag="VK031_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HLS52741.1"
                     /translation="MNKAELIASMAEKSNLTKKDAEAALNAFLQTVEEALVSGDKVQL
                     VGFGTFEVRARKAREGRNPRNPEEVIQIPASNAPVFKAGKNLKEAVNK"
     gene            5251..5493
                     /locus_tag="VK031_02135"
     CDS             5251..5493
                     /locus_tag="VK031_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HLS52742.1"
                     /translation="MRVDKFLKNSRIIKRRTVAREACLQGRVLINDKLAKPGDNLKVG
                     DVIKINFGNRTRVVEVLDIRDNVRKAEATTLYKNLD"
     gene            5557..5835
                     /gene="yabP"
                     /locus_tag="VK031_02140"
     CDS             5557..5835
                     /gene="yabP"
                     /locus_tag="VK031_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HLS52743.1"
                     /translation="MENTRKTFIKHNIVIEDRNRVKISGVEHVDSYNDTSISLFTIKG
                     KLSIKGEELNISKLNLDDGSIRISGTIDSLIYTGKEGEPRNLLGKIFK"
     gene            5848..6318
                     /gene="yabQ"
                     /locus_tag="VK031_02145"
     CDS             5848..6318
                     /gene="yabQ"
                     /locus_tag="VK031_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HLS52744.1"
                     /translation="MFELDLIGEFYIFLISISHGLVIGLIYDLHRALRYFSKPKKVRA
                     YIEDLLLWIIITALFFIFLLENTAGIVRGFVIIGFIVGFTFYLKTISRYSLPLIIKIF
                     KLILRLFNEIMKLITYPFRGVNVFVKKRTRRFYLLLKGVFKDMKKYRRIISRKK"
     gene            6340..6669
                     /locus_tag="VK031_02150"
     CDS             6340..6669
                     /locus_tag="VK031_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HLS52745.1"
                     /translation="MTRRRKKRRKKRFRLIHLILLVLCLYVFSTIINQRKLMKTMEHK
                     RASLQSDIKALEKEIDELNKEIENSDSLEFIEKVAREEMGMVKPREIIYIDKNKFKNS
                     ILNFFRK"
     gene            6741..>6828
                     /locus_tag="VK031_02155"
     CDS             6741..>6828
                     /locus_tag="VK031_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HLS52746.1"
                     /translation="MPVSVAEVVEGTVTGITNFGAFVQLSEGK"
BASE COUNT         2442 a          850 c         1437 g         2099 t
ORIGIN      
        1 gggcaaatgg gggaaaggca attggagcag gttatgttag attttattga taaaaagcag
       61 gatgtattgg tctgtactac cattattgaa acagggctag atatacctaa tgtaaatact
      121 attattgttt ataatgcaga taaaatgggc ctgtctcaac tttatcagtt aagaggcagg
      181 gtaggcaggt ctgacaggat tgcctatggg tattttacct atgaaaagga taaggttttg
      241 actgaagtgg cggaaaggag acttagggca attaaggagt ttacagaatt tggttctggt
      301 tttaagattg ccatgaggga tttagaaatc agaggtgcag gtaacctttt aggtgtagag
      361 caacatggcc atattgaagc aataggttat gacttgtatg tgaagttttt aggagaggct
      421 attagaaggc taaaaggtga aaaagttgta gaagcaatag acactacaat agatataaac
      481 attgatggtt atataccagt gaactatata gaagatgagg agctaaagat tgaagtatat
      541 aaaaagatag catccatatc tgatttggag gagtataggg aattaataga tgaattaatt
      601 gataggtttg gagatattcc tatagaggtt caaaacctaa tagatatttc ctatataaag
      661 gctttggcca gtagaaatca gattaaaaac ataagtcaaa ctaataatga aataaaatta
      721 gatttagtat ccaatgacaa acttccagtg gaattaatcc atcttttatc tgaaaaatac
      781 ggcaaggcca tgtcctttga tctatcctcc aatccctatt taaaattaaa aagtagcaaa
      841 aagctaatag ttgccctaag ggagctggtg gaacttattg atgattttca taaaaactaa
      901 aaagtaatat aaaatcctgg ctaaaaggtg aactatcgct aaactaatta ctttaagttt
      961 aacattaaca taatttttgt aatatgtata aaaatttcca cttggttgaa taataatcat
     1021 actacactta gtacgaggag gttaaatttt gaaagctaca gggatagttc gaagaataga
     1081 tgatttaggt agagttgttg ttccaaaaga aataagaaga accctgagaa ttagggaagg
     1141 ggatccttta gaaatattta cagatagaga aggccaggta atacttaaga aatattctcc
     1201 tataggtgaa ttaaatgaat ttgccggtga atactgtgaa tctctttatg ataatacagg
     1261 ccatacagct attatttctg atagagacaa tattattgcc atttctggtg ggtctagaaa
     1321 ggaatatttg gagaaaagaa ttagtcctga cctggaaaaa atagtagaat caagatcaac
     1381 ctttaccaca aatgacaaca gtcctattaa aatattttat gaagatgaaa gttccgatga
     1441 ctatgtggct caggtcatca ctcccattgt tatgcaggga gatccgatag gttctattat
     1501 cctcttaagt aaggaggctg gaactaagat gggggatttg gaggttaagc ttctggagac
     1561 agcggcagga tttttatcta aacagatgga acactaatac ctacattttt gtaggtattt
     1621 ttttattttt acatttatgc tatactctag caatgttata aaaaatttat atattaagga
     1681 ataaggacac atattataga ttaaggttca tattagatat gatataatat gagagataaa
     1741 ttattgaggt gatcctgttg tcgaagaata atagttttat taaaggtgca gctattctga
     1801 gcattgctgg aattatagtg aagattttag gtgccatata tagaatacct ttagccaata
     1861 taattaaaga tgagggtatg ggctattatc aaacagccta tcctttttat gttctgctac
     1921 ttactatctc aacttctggc tttccagtgg ccatagccaa gttagtttct gaaaaaaggg
     1981 ctctaggaga ccataggtct gcctataggg tatttaaggt ggccttaaca ggcttaacca
     2041 ttggtggcct gttaagctcc ctatttgtcc tatttatggc ggaggatata gtaagatcaa
     2101 tagacaatat aaatgcttat tactccttag ttgccttggt acctgcccta ttttttgttc
     2161 ctataatggc agcttttaga ggtttttttc aaggtagaaa aaacatggta cctactgccc
     2221 tgtcccagat aggtgaacaa tttttacggg tggtagttgg cttatccctt acttattatc
     2281 ttttagatag ggggattccg gtggcagctg gaggggcatc ttttggaggc tctgccggtg
     2341 caattttagg cacggtgata atttttataa tatttttatt acataaggag tccatagaaa
     2401 gggaagtcag acaaaccagg atacagacgg actactctgt tggagaaatt attaaggatc
     2461 ttttgaccat agccgttccc ataactattg gggcagccat tatacctact atggatacca
     2521 tagatgcagg cctggtaatt agaaggctac aatctattgg ttattctaaa gagacagcca
     2581 atgagttgta cggccagtta aaggggtttg ctcaaacctt aataaatcta cctcaggtat
     2641 tctctgtagc tataggtatg agcctggtac cggccatagc agatgctaat gctagaagaa
     2701 acaaaagaga aattaggcaa attataggat ctggcattag gatgacccta ttaattggtc
     2761 taccctgtgc ctttggctta tttgtgttag caaaacctat tataaatctt ttgtacttta
     2821 aaaatactat tgaaaccata aatagttctg gagaaatttt agcctactta tcctttggtg
     2881 taatattttt aactttggtt caggccctaa cggctatgct acagggactt ggtaagcctt
     2941 tgattcctgt aattaatcta tttataggag ctttactaaa aataattttg acttatatac
     3001 taactgctat tccgggcatt aatattaagg gggctgccat aagcactgta gtcgcatatg
     3061 caactgctgc tattttagac ttgatactct taagtaaggt tgcaaaaatc aaatttaatt
     3121 acagggatat atttattaaa cctttaattt cagccttagg catggccata attgttagat
     3181 tctcctatgg atttttatta aaaataattg gaggaaggct atcaactatc ttatccattg
     3241 gcattggagg gctggcttat gttatattat taatagtcac aggaactatt acagaggaag
     3301 atataaagtt tattcctaaa ggagaaaggc taggtaggta cctggataaa tttaaacttt
     3361 taaaataaaa acaggtaagg agataaaaag aaatggggaa gatttatata ataggtttag
     3421 gcccaggtag tgttgatcat ttaactctag gagccataga aagaattcac agtgggcata
     3481 aaaattttct aagaacagaa aaacatccta cagttagtta ttttttgaaa aacaatatag
     3541 actttgaatc ctttgattat ttatatgaca aggaggaaga ctttgaaggc ttatataata
     3601 atatagctga cagccttata aaagaggcca gggagagaaa ggttattaac tactttgtac
     3661 cgggcaaccc attggttgca gaaaagacag tagagatact tttacaaaaa gatatagaaa
     3721 cagagattat ctctggtatg agctttatag agcctatggt ggaaattgta ggaagagatc
     3781 ctataaatgg tctaaagata gtggacggat ctgattttga tagcctgatg attgatataa
     3841 atgtggatac aataataacc caggtataca atagaaggat tatgagcgat atcaaattga
     3901 tcctggctga aatttatggt gatgagtata atatttgggt gattaatggt gcaggcatag
     3961 aagaagagga aaaggtgcat aatattccta tatataacct ggatagaata gataaaattg
     4021 ggtatctaac cagtgtatac attcctagtg taaataatga aagggaaggt atatatgatt
     4081 tcaataatat agtaaaaatc ctaagaatcc taagaggaga aaatggttgt ccttgggata
     4141 taaagcagga tcacaaatcc ataaggaatt gtgtaattga agaagcctat gaggttgtag
     4201 atgccataga tagtggggat atagataact ttatagaaga gttgggagat ttacttcttc
     4261 aggttatttt tcatggtgag attgcaaatg atgaaggaga atttaactta atagatataa
     4321 catctaagtt ggcagaaaag ttaatttata ggcatccaca catttttttg gtaaaagagg
     4381 tagaaaatac ccaagaagta ggatataatt ggaatgagct taaggattct caacggggtc
     4441 ttagtagtgt agtagagaag atgacaagtc ttccagccct acctgcctta atgaagtcat
     4501 ataaaattca agatagggca gcccaggtag gatttgattg ggatgatata aggggaccat
     4561 tagataaaat tatggaagaa tatgaggaag tgcttgaagc cattgaaact aaggataagg
     4621 acaaaataga agaagagctt ggagatttat tatttgcagt tgtaaatctt gcaagatttc
     4681 taaaggtgga ccctgaagtg gccttaaatg gaaccaataa caaatttata aaaagatttg
     4741 ctattatgga aaaagaggca actaaggctg gcaaaaaatt agagcatatg tccttaagcc
     4801 agatggacct tctttgggat aaggccaagg aagataaaag ttagttataa aaagtttaac
     4861 tttttattaa aacttaatat attttggaaa ctattattta gggaggaatt tataatgaac
     4921 aaagctgaac taatagctag catggctgag aaaagtaact taactaagaa agatgcagag
     4981 gcagccttaa atgctttctt acaaactgtt gaggaggcat tagttagtgg agataaggtg
     5041 cagttagtag gatttggaac atttgaagtt agagctagaa aggctaggga aggtagaaac
     5101 ccaagaaatc ctgaagaagt tattcaaata cctgcatcaa atgcaccagt atttaaagct
     5161 ggtaaaaact taaaggaagc tgttaataaa taggagttaa aaagtcaggt cttggacctg
     5221 acttttttag cataattaag gagtggtagt attagagtag ataagtttct caaaaattcc
     5281 aggattatta aaagaagaac agtagctaga gaggcttgcc ttcagggacg ggtacttatt
     5341 aacgataaac tggcaaaacc cggagataat ttgaaggtag gggatgtaat aaagattaat
     5401 tttggaaata ggacaagggt agtggaggtt ttggatataa gggataatgt aaggaaggct
     5461 gaagctacaa ctttatataa gaatttagat tagggctttt gtaataaaag ccctttttta
     5521 ttcataaata ttattaaaga tagggagggg gaataaatgg agaatactag aaaaactttt
     5581 ataaaacata atattgttat tgaagacaga aaccgggtaa agatctcagg ggtagagcat
     5641 gtagatagtt ataatgatac aagtataagt ctttttacaa taaaaggtaa gctaagtatt
     5701 aagggagaag aacttaatat aagtaaactg aatttagatg atggatctat tagaatatca
     5761 gggacaatag acagccttat ttatactggc aaagaaggag aaccaagaaa tttattaggc
     5821 aagatattta aataagctgg tgctattatg tttgaactag atttaatagg tgaattttat
     5881 atttttttaa tatctattag ccatgggcta gtaatagggc ttatttatga cttgcatagg
     5941 gcattaaggt attttagtaa acctaaaaag gtcagggctt atattgagga cctactgtta
     6001 tggattataa taacagccct attttttata tttttattgg aaaatacagc aggtatagta
     6061 aggggatttg taataatagg tttcatagtt ggctttacct tttatttaaa aacaatatct
     6121 agatatagtt tgccactaat aataaaaatt tttaagttga tattaaggct atttaatgag
     6181 ataatgaaat taataactta tccttttaga ggagtaaatg tatttgttaa gaaaagaaca
     6241 agaaggtttt acctcctatt aaaaggggta tttaaagata tgaaaaaata cagaaggatc
     6301 atatctagga aaaagtgact tgatactggt ggtgaaacta tgactaggag aagaaaaaaa
     6361 agaaggaaga aaagatttag gttaatacac cttatattac tagtcttatg tctttatgta
     6421 ttttcaacca taattaacca aaggaaacta atgaaaacaa tggagcataa aagggccagt
     6481 cttcaatctg atataaaagc tttggaaaag gaaattgatg agctaaataa agaaattgaa
     6541 aatagtgatt ccttagaatt catagaaaaa gtagccaggg aagagatggg aatggtaaaa
     6601 cctagagaaa tcatatatat tgataagaat aaatttaaaa attctatttt aaacttcttt
     6661 agaaagtaga ttcattgaca tatttaaaat tctaatatat actaaaatta agaatatttt
     6721 taaggaggaa ttatttattt atgcctgtat ctgttgcaga agtagttgag ggcactgtaa
     6781 ctggcattac taattttggt gcatttgttc aattgtcaga aggtaaaa
//
LOCUS       DATGSI010000136         1813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_200727, whole genome shotgun sequence.
ACCESSION   DATGSI010000136 DATGSI010000000
VERSION     DATGSI010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200727"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1280)
                     /locus_tag="VK031_02160"
     CDS             complement(102..1280)
                     /locus_tag="VK031_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLS52747.1"
                     /translation="MKRNFLIVLSLILVLSLVAGCTSSPASNGGGKDGDTIRIGLNFA
                     ASGFAATYGQGLTKGIELAFEEINEAGGLFDKKFEAVKFDNKSDDTEAANISTRLATR
                     ENVVAMLGSDISGTTKAAIPAAMENKIPLISGSATADDVTIDNNGNVREYVFKTCFND
                     SFQGVMMAEFAYHELGFLKAAILSDTTSDYAKGLSKSFRETFEGLGGQVLVEEFYQEN
                     DTDFRAVLTNINGKDPEVLFVPGYYEEVGLIIRQARELGLDVPILGADGFESPKLVEI
                     AGEGVLNDVFYSSHYSPMDDAEEVVKFREAYKEKYGEDPDAFNALGYDLAYLLKDALE
                     RAGEVDSEKLKDALATTENFTGVTGTLSMDEFHNPVKSVTINELKNGEVTFYKKIDPQ
                     "
     misc_binding    complement(1349..1606)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          533 a          387 c          258 g          635 t
ORIGIN      
        1 tatgccatta attaattgtt gtataatctg ttccatcttt aacttccttt cattagaata
       61 atgtctttaa agaaaacctt taaagacatt attcttataa actattgagg atctattttc
      121 ttatagaagg tcacttctcc gttcttcagt tcatttatag ttacagattt tacaggattg
      181 tggaactcat ccatagataa ggtaccagtt actcctgtaa aattttcagt agttgccaat
      241 gcatccttta atttttcgga atctacttct cctgcccttt ccaaggcatc cttcaatagg
      301 taggctaagt catatcctag ggcattaaat gcatctggat cctctccata cttttcttta
      361 tatgcttctc tgaattttac tacctcttct gcatcatcca ttggagaata atggcttgaa
      421 tagaatacat catttaatac accttcacct gcaatttcaa ctagcttagg agattcaaat
      481 ccatctgccc ctagaatagg tacatctaaa cctaactctc tggcctgcct aatgattaac
      541 ccaacctctt cataatagcc tggtacaaac aatacctctg gatcttttcc atttatattg
      601 gttagtacag ctctaaaatc tgtgtcattt tcttgataga attcttctac tagtacctgg
      661 ccacctaggc cttcaaaggt ttctcggaaa ctctttgata atcctttggc ataatcggaa
      721 gtagtatcac ttaatattgc tgcctttaga aagcctaatt catggtaggc gaattctgcc
      781 atcataacac cttggaaaga atcattaaag caggttttaa atacatattc cctaacattt
      841 ccattattat ctatagttac atcatctgca gtggctgatc ctgagattag agggattttg
      901 ttctccatag ctgcaggaat agccgccttt gtagttccag aaatatcaga gcctagcatg
      961 gctaccacat tttctctagt agctaatctt gtggaaatat tagcagcctc tgtatcatca
     1021 gacttattat caaatttaac tgcttcaaat ttcttatcaa ataggccacc agcttcatta
     1081 atctcctcaa aggccaattc tatacctttg gtcaatcctt gaccataagt agcagcaaag
     1141 cctgaagctg caaagtttaa acctattcta atggtgtcac catccttgcc tccaccatta
     1201 ctggctggtg aagatgtaca tcctgccaca agacttaaga ctaaaatcaa agataatact
     1261 attaagaaat tccttttcat ctataacacc tctcctattt tcattactaa attgggtatt
     1321 aaaaaagccc cacgtgtatt taggtacacg tagaggccat taagcgatac cactctacct
     1381 tataattatc tcgcgataat tatctcacta ggttccatag atatggctag ccacttccat
     1441 aatctattaa accttgacct gataacgggg tctaccggta gggcctactt ttttcagcca
     1501 tacaactccg aagtgatagt tccctattaa accaaggttg gctttcacct gatccaactc
     1561 tctgtacttg gtatgataat agttcactct tcatcctggt cttttacctg tttaactact
     1621 ttggattgca atccgttttt gatatttaat gaataatata gcatgttaaa gtactactgt
     1681 caagagtttt ttgtaaaatt tagaaaatat tttaataatt tgctttttta tcagtttaaa
     1741 tcgttaaata tatgaaattc tatcttttgc aatgttagta aacctttaaa aattaaaatt
     1801 ttctccctca tag
//
LOCUS       DATGSI010000137         4874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_244175, whole genome shotgun sequence.
ACCESSION   DATGSI010000137 DATGSI010000000
VERSION     DATGSI010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244175"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VK031_02165"
     CDS             <1..235
                     /locus_tag="VK031_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HLS52748.1"
                     /translation="SIPALLAKEDDVEGDHAVTAGQLDESKLFYLMSRGLDEQAAKKL
                     IVESSFWPIIEKIPSTSLRQLVTEEVERRLING"
     gene            228..1463
                     /locus_tag="VK031_02170"
     CDS             228..1463
                     /locus_tag="VK031_02170"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HLS52749.1"
                     /translation="MDKLNPEKLRADFPILDQKVHGKRLIYLDNAATTHKPQAVIEAM
                     AEYSRKKHGNPHRGAHYLSVAATEEYEKSKEVVKKFINAQSSEEIIFTRNTTESINLI
                     AQSYGMAFVNEGDEILLAISEHHSNILPWQRVAQVKGATLKYMYLDQDGRLPACEFEK
                     ITSRTKILSIGQMSNVLGVIHPIKKLIEKAHKLGAVVVIDGAQSAPHMQVDVQDLDAD
                     FFVFSAHKMLGPMGIGVLYGKKELLETMPPYLLGGDMIEYVYEQESTFAPLPYKFEAG
                     TQNVEASIGLREGIKYLEAIGMEKIYEHELRLTEYCLAKLSELPYVQIQGPRDLSLRG
                     GIISFTIDDVHPHDVATILDSYGIAIRAGHHCAQPLMRYMDLPATSRASFYFYNTEDD
                     IDIFIEGLKNVRKWLGYGS"
     gene            1453..1875
                     /locus_tag="VK031_02175"
     CDS             1453..1875
                     /locus_tag="VK031_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HLS52750.1"
                     /translation="MDLNSIYTQVIMEHNRSGHNKRSLDNPDRIERGHNPSCGDDIAL
                     EVKLNGDIIEDASFTGVGCAISQASTSIMVDLIKGKSKDQAMDLADTFLKMIKKEIKD
                     EKELEKLGDAVILENISNMPARVKCAVLSWHTLKEALS"
     gene            complement(1915..2148)
                     /locus_tag="VK031_02180"
     CDS             complement(1915..2148)
                     /locus_tag="VK031_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004836994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HLS52751.1"
                     /translation="MKKLKLFYFDECPYCQKVIGYMKENNIEVEMANIQTDQKNREEL
                     IEIGGKVQVPMLSIDGEALYESDDIIEWFKNNI"
     gene            complement(2204..2410)
                     /locus_tag="VK031_02185"
     CDS             complement(2204..2410)
                     /locus_tag="VK031_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52752.1"
                     /translation="MFKIMFFPLIILGKLVKIFMKIAARLLGLSLGLVFFFVGLICTF
                     TIVGAVIGIPLIILGLWILFKSIF"
     gene            complement(2462..3811)
                     /locus_tag="VK031_02190"
     CDS             complement(2462..3811)
                     /locus_tag="VK031_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HLS52753.1"
                     /translation="MKFWKDKVFLKSMIVIAIPIALQNLITSSLNMVDTLMITSLGDA
                     SIAAVGLANQVFFFYALINFGIHTGSSIFISQYWGKKDIINIRKVLGLAITLSAITGV
                     IFTVAAFFFPELIMSILIKDAEVIQLGVDYLRIVSLSYIITGIAFAYSIALRSTGRPN
                     VPMMVSAISFVTNTVFNYLLIFGKFGFPELGVKGAALGTLIARIVEIIFVLYVVYSNV
                     GPLSATLAELFDWSREFVNRYIKTIAPVILNEAFWSLGQIMYAIAYARIGKEATAAIQ
                     LTTNIQNIFYVLVRGLANACAVMVGNKIGAQDEEGAYDYAIKFLSISTTIGLVLGVIM
                     VLTPDLTLKIFGDLSPELYMSARNLLIIMGFAFFIRAYNSTAIVGVLRGGGDTKFSMF
                     LEMGAVWLVGVPLAFIGALVLKIPVYYVFLLVSLEEVVKFLVSIPRILSKKWIQDLT"
     gene            complement(3896..4429)
                     /locus_tag="VK031_02195"
     CDS             complement(3896..4429)
                     /locus_tag="VK031_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLS52754.1"
                     /translation="MKFQRKGITLVELLVTLAIVSMAIMLVGSFYFTGSKSFERSRDS
                     SLAQGDARFVTDYIKRELKTAKNVYLKEENIEELSEGYYTLKIDKRRNLEITHSEDAN
                     KNKRFNIPVESIEFKLTKDGKNINRDRLKLMVVANEDGYERKYKSDIYFENGNVIYGS
                     HDELARIETIYYTRYDD"
     gene            complement(4426..4800)
                     /locus_tag="VK031_02200"
     CDS             complement(4426..4800)
                     /locus_tag="VK031_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLS52755.1"
                     /translation="MKKRKGLTLIETILSLAILGIIGVFILTVFGFSLNNIRRAGKRT
                     EDVFQLGDEIDKIIFKSTDEAEDGIVRKISQDEKTLVHIPGIGDKEVRGMKVTVELKD
                     KNRTRITTFVPYEEEEKGRFDR"
BASE COUNT         1571 a          934 c          823 g         1546 t
ORIGIN      
        1 ctctatacct gcccttttgg ctaaggaaga tgacgtggaa ggggatcatg ctgtaacagc
       61 aggtcaactg gatgaaagta agttgtttta cctgatgagc agaggcttag acgagcaggc
      121 agcaaaaaaa ctaattgtcg aatcttcatt ttggcccata attgagaaaa tcccttcaac
      181 aagtctacgc caattagtaa ctgaggaagt tgaaaggagg ctcattaatg gataagttaa
      241 atcctgagaa acttagagcc gactttccca tattggacca aaaagtccac ggcaaaagat
      301 taatatattt agataatgca gccaccaccc ataagccaca ggctgtaatt gaggctatgg
      361 ctgaatacag tagaaaaaaa catgggaacc ctcatagagg ggcccattat ttaagtgtgg
      421 ctgccactga agaatatgaa aaatcaaagg aagtagtaaa aaaatttatc aatgcccaaa
      481 gcagtgaaga gattatattc accagaaata ctactgaatc tatcaactta attgcccaat
      541 cttatgggat ggcttttgta aatgaaggag atgaaatcct gctggccatt tctgaacatc
      601 atagcaacat cttaccttgg caaagggtgg cccaagtaaa aggtgccact ttaaaatata
      661 tgtatttaga tcaagatggg aggctgccgg cctgtgaatt tgaaaagatt acttccagaa
      721 ctaaaatttt aagtataggg caaatgtcta atgttttagg ggttatccat cctattaaaa
      781 aactaataga aaaggctcat aagctagggg ctgtagttgt aatagatggg gcccaaagtg
      841 ccccccacat gcaggtggat gttcaagatt tagatgcaga ttttttcgtt ttctcagccc
      901 ataaaatgtt aggacctatg ggcattggag tactatacgg caaaaaggaa cttttagaaa
      961 ctatgcctcc ctatctatta ggtggagata tgattgaata tgtctatgaa caagaatcca
     1021 cctttgcccc cctaccttat aaatttgaag caggaacaca aaatgttgag gcttctatag
     1081 gacttaggga gggaataaag tacttagagg ccatagggat ggaaaaaatc tatgaacatg
     1141 agttaaggtt gacagagtat tgtttagcaa agctttcaga attaccctat gtgcaaattc
     1201 aaggcccaag agacttaagc ttaaggggag gcataatttc ctttaccata gacgatgtcc
     1261 atccacatga tgtggctacc atattggatt cctatggaat cgccattagg gcaggccatc
     1321 attgtgctca acctttaatg agatatatgg atctaccagc cacatctagg gctagttttt
     1381 atttctacaa taccgaggat gatatagata tttttataga aggattaaag aatgtaagga
     1441 agtggttagg ttatggatct taattcaata tatacacaag tgattatgga acataataga
     1501 agcggtcaca ataaaaggtc attagataat ccagatagga ttgaaagagg ccataatcct
     1561 agttgtggag atgatatagc cttagaggta aagttaaatg gagatattat agaagatgct
     1621 tcctttactg gagtaggctg tgccatatcc caggcttcca cttccataat ggttgactta
     1681 atcaagggta agagtaaaga ccaggctatg gacctagccg ataccttcct taaaatgata
     1741 aaaaaggaaa taaaagatga gaaggaattg gaaaagctgg gagatgcagt tattctagaa
     1801 aacatatcta atatgccagc cagggtaaaa tgtgctgttt tatcttggca caccttgaag
     1861 gaagctttat cttaacaaat aatcccctgg gaagaaatcc caggggatta tttgttatat
     1921 attattttta aaccattcta taatatcgtc tgactcatat agggcctcac catcaattga
     1981 taacatgggc acctgtactt ttccacctat ttcaattaat tcttccctat tcttttgatc
     2041 tgtttgaata ttggccattt ctacctctat gttattttcc ttcatatagc ctataacttt
     2101 ttggcaatag ggacactcat caaagtaaaa caactttaat tttttcattc taatctctcc
     2161 ttcaaccttt atctatccta taatataccc agcctacctt agtttaaaag attgatttaa
     2221 ataaaatcca aagccctaat ataatcaatg gtatcccaat tacagctcca actatggtaa
     2281 aggtacatat gaggcctacg aagaaaaaca caagcccaag gcttaggcca agtaatctgg
     2341 ctgctatttt cataaaaatt ttaactagct tgcctaatat aattagggga aaaaacataa
     2401 ttttaaacat gccgaccatc caatctaaat ataagctaca tacttaaagt tttcttctta
     2461 cttatgttag gtcctgtatc catttcttag ataaaatcct gggaatggaa actaaaaatt
     2521 taactacttc ttccaaggat actagaagaa atacataata aactgggatt ttaagcacca
     2581 aggcaccaat aaaggctaaa ggaactccca caagccacac cgcccccatt tctaaaaaca
     2641 tggaaaactt agtatctcca ccacctctta ggacaccaac tattgccgtt gagttataag
     2701 ccctgataaa gaaggcaaat cccattatta ttagtagatt cctagcagac atataaagct
     2761 ccggacttaa atctccaaat atctttaatg ttaaatctgg tgtcagaacc atgattacac
     2821 ctaagactag tcctatagtt gttgaaatgc taagaaattt aatagcataa tcataggctc
     2881 cttcctcgtc ttgagctcct atcttatttc ctaccattac agcacaagca ttggccaggc
     2941 cccttactag aacatagaat atgttttgta tattggttgt aagctgaata gctgcagtgg
     3001 cctccttacc aattctagca taggctatgg catacattat ttggcccaag gaccagaagg
     3061 cttcatttaa aattacaggt gcaatagttt ttatatacct gttaacaaac tctctactcc
     3121 aatcaaacaa ttcagctaag gtggctgata agggccctac atttgagtaa accacatata
     3181 gtacaaatat gatttctact atcctggcta tcaaggtccc cagagctgcc cctttaactc
     3241 ctaattctgg aaagccaaat ttaccaaata ttaataggta attaaatact gtatttgtaa
     3301 caaaggagat ggcagacacc atcataggta cgtttggcct gcctgtactt cttaaggcta
     3361 tggagtaggc aaaagctatt cctgttatta tataggatag ggatactatc cttaagtaat
     3421 ccacacccag ttgtattact tctgcatcct taatcaatat actcatgatc agctctggaa
     3481 agaagaaggc tgccacagta aatattactc ctgttattgc acttaaagta attgccagcc
     3541 ccagcacttt tcttatattt attatatctt tcttccccca atattgggat ataaaaattg
     3601 atgatcctgt atggatccca aaattaatta gagcataaaa aaagaatacc tggtttgcca
     3661 agcctacagc tgctatactg gcatcaccta gacttgtaat catcagggta tccaccatgt
     3721 ttaatgatga tgtaattaga ttttgtagtg caataggtat tgcaataaca atcattgatt
     3781 ttaaaaaaac tttatccttc caaaatttca tataagtcct cctgaaataa taaacgttct
     3841 acatgcaata aaaggcataa tagaacgtca gttttgtatt tgtttgtggt taaatttaat
     3901 catcatatct agtataatat attgtttcaa ttcttgcaag ttcatcatgg cttccgtaaa
     3961 taacattacc attttcaaag tatatatctg acttatattt tctttcatat ccatcttcat
     4021 tggccactac cattaacttg agcctatctc tatttatgtt ttttccatcc ttagtcaatt
     4081 taaattctat agattctaca ggaatattaa atcttttatt tttattagca tcttctgaat
     4141 gtgttatttc taggttcctt cttttatcta tttttaaggt atagtagcct tctgatagtt
     4201 cttctatatt ttcttcctta aggtagacat ttttagcagt tttcaattct ctttttatat
     4261 agtcagttac aaatctggca tctccttgag ccaaacttga atccctgctt ctttcaaagg
     4321 atttagatcc ggtaaaataa aaggagccta ctagcattat tgccatagat actatggcca
     4381 aggttacaag tagctcaact agggttatcc cttttctttg aaatttcatc tatcaaacct
     4441 acctttctcc tcttcttcat agggtacaaa ggtagttatt ctagttctgt ttttatcctt
     4501 aagttctacg gttactttca tgcctctaac ttctttatca cctattcctg gtatgtgaac
     4561 aagcgttttt tcatcttggc tgatctttct tactatccca tcctctgctt catctgttga
     4621 tttaaaaata atcttgtcta tctcgtcccc taattggaat acatcctctg tccttttccc
     4681 agccctcctt atattgttta agctgaaacc aaatactgtc aaaataaata cacctattat
     4741 tcctaaaatg gccagggaca aaattgtttc tattaaggta agtccttttc tttttttcat
     4801 aaaatcacct ttatctataa taaccaggag taaaattaat gctatctctt gttattggac
     4861 taattacagg tgga
//
LOCUS       DATGSI010000138         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_577781, whole genome shotgun sequence.
ACCESSION   DATGSI010000138 DATGSI010000000
VERSION     DATGSI010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577781"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..443
                     /locus_tag="VK031_02205"
     CDS             177..443
                     /locus_tag="VK031_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52756.1"
                     /translation="MITAKDCKNCGEDKLKALLCKISRELSIISKKRYNNDRFNLGRD
                     IDERRFKALLNYKNILYKKAFDSTYTGANTNYIISKIKSYIYAR"
     gene            433..1470
                     /locus_tag="VK031_02210"
     CDS             433..1470
                     /locus_tag="VK031_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52757.1"
                     /translation="MQGNCCDDNKCGGFVYSSECVVYEGDLGPEVQGNKGDSLTSIID
                     NIITNIGYTETGNINVEDLKLSTCQIIKDKTGGSKDLDKVLQALIDSHCELKTYVENR
                     TKEDIPSYDLKCLSPASNSEEDIIQSIINQTCSTKDTVTNLENTVEENTTEIDSIATK
                     VAAIMGDLNSQDNFSFTFSQLAPPYVPMPYIGDLDNFDGNGKGRESKGFKNVYIMDGR
                     NGTPDWRGYSPVGATTTGYTSSLDSRVTSLNLALSQKKGEVNHKLTSGEMPKHNHGIT
                     DLGHTHLYHGHKSTSARAKAGGGGMDEFHTKSAYTAPSKTGITINKTGGGNTHNNIQP
                     SVGVIWIIKLP"
BASE COUNT          587 a          197 c          287 g          439 t
ORIGIN      
        1 agattgtagt gaacaaaatt gtcactcaga ggaagagtta gataaagctt atatgtattt
       61 acaaggagca aaggcaaatg caagtgtttg taattatagg ctggctaaca agctttataa
      121 aaaatctaat aaaatattat catctatatt aggagatagc aataattgtt aatattatga
      181 taacagcaaa agactgtaaa aactgtggag aggataagct gaaggctctc ttatgtaaaa
      241 taagtagaga attgagtatt atatcaaaga aaagatataa caatgacaga tttaacttag
      301 gtagggatat agatgagaga aggtttaaag ctttactaaa ttataaaaat attttatata
      361 aaaaagcgtt tgatagtact tatactggtg ctaatactaa ttatattatt tcaaaaataa
      421 aaagttatat atatgcaagg taattgctgt gatgataata aatgtggtgg ctttgtttac
      481 tcctcagagt gtgtagtata tgagggagat ttaggaccag aagtacaagg gaacaaagga
      541 gactctttaa cctctataat agacaatatt attaccaaca ttgggtacac agagacaggt
      601 aatattaatg ttgaagattt gaaactttct acttgtcaga taataaaaga caagacagga
      661 ggaagcaaag atcttgataa agtactacag gctttaatag actctcattg tgaattaaaa
      721 acttatgtag agaatagaac aaaagaagat atcccctctt atgatttaaa atgtttatct
      781 cctgctagta actcggaaga ggatattata cagagcatta taaatcaaac atgtagtact
      841 aaagatacag tcactaattt agagaacact gtagaggaaa atactactga aatagacagt
      901 attgcaacca aggttgcagc aataatgggt gacttaaatt cccaagataa cttctctttt
      961 acattttctc aattagcacc tccttatgtc ccaatgcctt atattggtga tttagataac
     1021 tttgatggaa atggtaaagg aagagagagt aaaggattta agaatgtata tatcatggat
     1081 ggaagaaatg gtactccaga ttggaggggc tattctccag taggtgctac tacaacagga
     1141 tatacttcct ccttagatag cagggtgact agcttgaatt tagctctttc tcagaagaag
     1201 ggagaagtga accacaaatt aacttcagga gaaatgccta agcacaatca tggtattaca
     1261 gatttaggcc atactcattt atatcatgga cataaaagta cttcagcaag agcgaaagca
     1321 ggcggaggag gaatggatga gtttcatact aaatcagcat acactgctcc ctctaaaaca
     1381 ggaataacaa ttaataaaac aggtggaggg aatacacata acaatattca accttctgtt
     1441 ggtgttattt ggattatcaa attaccttaa atattaaaaa atataataat tatgaaaagt
     1501 gatattaata
//
LOCUS       DATGSI010000139         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_153239, whole genome shotgun sequence.
ACCESSION   DATGSI010000139 DATGSI010000000
VERSION     DATGSI010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153239"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VK031_02215"
     CDS             <1..214
                     /locus_tag="VK031_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52758.1"
                     /translation="YVIDVINVAVENFAPKVHTHTIEQVTGLQGELNGKANSLHAHSI
                     SDISGLEERLEAIESRLDALEEPEEG"
     gene            264..644
                     /locus_tag="VK031_02220"
     CDS             264..644
                     /locus_tag="VK031_02220"
                     /inference="COORDINATES: protein motif:HMM:NF017205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52759.1"
                     /translation="MRSKFEKEVAKYLKQNKVKFEYEPKDKIVNYQIPVTNHKYTPDF
                     VIDGVMYETKGYIRSVAERRKYVYVAEQNPDLKLVMVFQNPNLPIRKGSKTTYKTWFE
                     KNGIQTMSIKEFKKKYENKTKSTK"
     gene            616..1320
                     /locus_tag="VK031_02225"
     CDS             616..1320
                     /locus_tag="VK031_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52760.1"
                     /translation="MKTKLRVLSESDGYQIKLVDDTGFQNQYNLEGYKETPEMVEYVF
                     EVFDEVRRELFTFNETDVEPYMNRVVKTLEMDKLFKDSLYKITMYSVFDIGGVGDGFE
                     GLSFISNVSGAESIYNNYTAIKAGDYVYQIVGRSGDVLLLDRDIVEDFEEFMLVIHSS
                     DKVALLSEIEDSIMGVIRTLGSCKCKGKVDKVAELQLYKHGIELAVESSNYGLAEELL
                     GFMTDVSGQLKGCSRC"
     gene            1314..1496
                     /locus_tag="VK031_02230"
     CDS             1314..1496
                     /locus_tag="VK031_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52761.1"
                     /translation="MLSKDLREGLLDRIKRMKVGRFNSLKLFVLVSAKEMVDNSNVKY
                     LDNTVDRLLKKEIKNV"
BASE COUNT          605 a          203 c          378 g          430 t
ORIGIN      
        1 gtatgtaata gatgtaataa atgtcgctgt agaaaacttt gcacctaagg ttcatacgca
       61 tacaatagag caagtgacag gactacaggg agagctgaat ggtaaagcca attctttaca
      121 tgcacacagc atctctgaca ttagtggact agaagaaaga ttagaagcta tcgaatccag
      181 attagatgct ttagaagaac ccgaagaagg gtagtcaacc cctaacagat ggttaaggta
      241 atattctgta tatttgtaac attatgagga gtaagtttga gaaggaggtg gctaaatacc
      301 ttaaacagaa taaagtaaag tttgagtatg agcctaagga taagatagtt aattaccaga
      361 taccagtcac taatcacaag tacacaccag actttgtgat tgatggggtt atgtatgaga
      421 ctaagggata cattaggagt gtggcagaaa gacgtaaata cgtttatgta gccgagcaga
      481 atccagactt aaaattggta atggtatttc aaaaccctaa tctccccata agaaagggaa
      541 gtaagactac ttataaaacg tggtttgaaa agaacggaat acagactatg agtattaaag
      601 aatttaaaaa gaagtatgaa aacaaaacta agagtactaa gtgagtcgga cggctaccag
      661 attaagctag tagacgacac aggttttcaa aaccaataca acttagaagg atataaggaa
      721 acccctgaaa tggtagagta cgtctttgaa gtgttcgatg aagtaaggag ggagcttttt
      781 acatttaatg aaacagacgt agagccttac atgaataggg tggttaagac gttagagatg
      841 gataagctgt ttaaggattc gttgtacaaa ataaccatgt actctgtatt cgacattggt
      901 ggtgtaggag atgggtttga aggactttct tttatatcta acgtttcagg agcagagtct
      961 atttataata actacaccgc tattaaggca ggtgattacg tgtatcagat agtcggtaga
     1021 agcggagacg ttttactatt agacagagat attgtagagg attttgaaga gtttatgtta
     1081 gttattcatt cctcagataa ggtagctcta ttaagcgaga tagaagatag tataatggga
     1141 gtcattagga cacttggtag ttgtaaatgt aagggtaaag tagataaggt agctgaatta
     1201 cagttataca aacatggaat agagcttgca gtagaaagct ccaactacgg actagcggaa
     1261 gaactgttag ggtttatgac agatgttagc ggacaattaa aagggtgttc gagatgttaa
     1321 gtaaagattt aagggaagga ctgctagaca gaataaaaag gatgaaagtc gggaggttta
     1381 atagcttaaa gttatttgta ctagtttcag ctaaggaaat ggtagacaac agtaacgtta
     1441 aataccttga taacacggta gatagattac ttaaaaagga aattaagaat gtctaagaaa
     1501 agagataata aaagacctaa ttttacccta aatcaaagaa ttgttgaggg aaaagagtct
     1561 gacgtaattt ggagaaagtc taataaaata gtaaaagaga aaatagttta tgaaaa
//
LOCUS       DATGSI010000140         1085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_200894, whole genome shotgun sequence.
ACCESSION   DATGSI010000140 DATGSI010000000
VERSION     DATGSI010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200894"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VK031_02235"
     CDS             <1..250
                     /locus_tag="VK031_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HLS52762.1"
                     /translation="FDDGDIIVSVSTDKDMVNFMERASAIITEHGGLTSHGAIVGLNL
                     NKPTIVGAENATNILKDGDIITVDSSTGQIYKGEARVL"
     gene            492..>1085
                     /gene="dusA"
                     /locus_tag="VK031_02240"
     CDS             492..>1085
                     /gene="dusA"
                     /locus_tag="VK031_02240"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HLS52763.1"
                     /translation="MTRISIAPMVDVTDRHFRYFCRLLTKYSILYTEMITSSAIIHGD
                     RNKLLDFNPIEKPLSLQIAGHDREEVKEAVRIAEAWDYDEINLNVGCPSDRVSGNHMG
                     ASLMAYPDLVAELVEAMREETEKPITIKHRIGIDGRNVLPDSLDKLVIDEYDDLKKFV
                     EKIKRAGVNHFVIHARIAILEGLSPRQNREIPPINYDM"
BASE COUNT          398 a          133 c          240 g          314 t
ORIGIN      
        1 atttgatgat ggagatatta tagttagtgt aagtacagat aaagacatgg taaacttcat
       61 ggaaagggcc tcagctatta taacagagca tggaggatta acatcccacg gagccattgt
      121 aggactaaac cttaataaac ctaccatagt aggtgcagaa aatgcaacta atattttaaa
      181 agatggtgac attattacag tggatagctc aactgggcaa atctacaagg gagaagctag
      241 agttttataa tattttagat aaagggcgag gttactcgcc ttttttattt aagtattcaa
      301 gatgcattta taggaaatac tataaaaaaa gaaggagtag aattaggata taatctagat
      361 tatggataat aagttgaagt taataattga ccataatatg gcttaagcag tcttaaaaaa
      421 attatctggt ataagttaat atttgtggta aaataataaa ttagatttat aattttaagg
      481 ttgtgatgaa gatgactaga attagtatag cacctatggt ggacgtaaca gacaggcatt
      541 ttagatattt ttgtaggctg cttaccaaat actctatctt gtatacagaa atgatcacta
      601 gcagcgcaat aatccatgga gatagaaaca agttattaga ttttaatcct atagaaaagc
      661 ctttaagttt acagattgct gggcatgata gggaagaagt gaaagaggct gtaaggattg
      721 cagaagcttg ggattatgat gagataaact taaatgtggg ttgtccctca gatagggtct
      781 ccggtaatca tatgggagcc agtttgatgg cttaccctga cctggtggca gagctggtgg
      841 aagccatgag ggaggagacg gaaaagccta taactattaa acatagaatt ggaattgatg
      901 gaagaaatgt tttaccagac tctttagaca aactagttat agatgagtat gatgatttaa
      961 agaaatttgt agaaaagatt aaaagggcag gagtaaacca ttttgtaatc catgcaagaa
     1021 tagccatatt agaaggttta agtcctaggc aaaataggga aattcctcca ataaactatg
     1081 atatg
//
LOCUS       DATGSI010000141         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_672048, whole genome shotgun sequence.
ACCESSION   DATGSI010000141 DATGSI010000000
VERSION     DATGSI010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672048"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /gene="prxU"
                     /locus_tag="VK031_02245"
     CDS             complement(<1..664)
                     /gene="prxU"
                     /locus_tag="VK031_02245"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083760882.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(419..421),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HLS52764.1"
                     /translation="MTKKMEIGCARPKISKSSEEVVTEEKTIIKKEEQNMIKVGKPAP
                     QFTAPAYHNGEFTEVSLEDFKGQWVLLCFYPGDFTFVUATEVAAVAEKHDEFKDLGVQ
                     VLSVSVDSVFVHKIWNDHELSKMVNKNIPFPMLSDQDGSIGKMYGIYDEDSGVETRGR
                     FIIDPDGIVQGYEVLTPPVGRNVAETLRQIKAYQFVREAQETEVTPAGWQPGKTTLKP
                     GPD"
     gene            complement(844..>2397)
                     /locus_tag="VK031_02250"
     CDS             complement(844..>2397)
                     /locus_tag="VK031_02250"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HLS52765.1"
                     /translation="AGLRGKIGAGTNRMNKYTVALATQGLAQTIINKGQEAMDKGVAI
                     AYDVRRYSDKFAEIAARVLAANGIKVYLFDGIRTTPLLSYTVRRLKTISGIVITASHN
                     PKDYNGYKVYWEEGSQILDEIGDEILAEIERIRDYEDIKLMDLAEAKQKNLISYIGKE
                     IDDEYNETRLKEAINEDIEKDIKIVYTPLNGTGNIPVRRILKDRGFTNIYVVPEQELP
                     DSNFTTVEYPNPEDPVAFEYAIRLGKKKDADLLIATDPDCDRIAIMVKNKAGEFTFLN
                     GNQTGALLVHYILSSRKRNNNLPANGAMVKSIVTSDLGTAIAEDHGVKMFETLTGFKF
                     ICSKANEWESTGEYDYIFGYEESIGYTYGTLVRDKDAIVSAMLIAEMAAYYKKKGKDL
                     LKVLDDIYKKYGYYKERLISIVLEGMEGSRRIKRIMEEFRQAPIKTIGDMKLVKVIDY
                     LHDETGLAKSNVLKYFLDDGSWYAIRPSGTEPKIKLYAYSKDKKEEKAEKKVKLIEKT
                     VREKMDKVK"
BASE COUNT          625 a          529 c          327 g          916 t
ORIGIN      
        1 gatctggtcc tggttttagt gtagtctttc ctggttgcca gccagcagga gttacttctg
       61 tttcctgagc ctctcttaca aactggtaag ccttaatttg tctaagagtt tcagctacat
      121 ttcttcctac tggaggtgta agtacttcgt acccttgtac tattccatct gggtcgatga
      181 taaatcttcc cctagtttct actccactat cttcatcata gatgccatac attttaccta
      241 tgctaccatc ctgatctgac agcatgggga agggtatgtt tttgttaacc atttttgata
      301 gttcatggtc attccaaatc ttgtgaacaa atacagagtc tacacttact gataatacct
      361 gtactcctaa atccttaaat tcgtcgtgtt tttcagcaac tgctgcaact tcagtggctc
      421 agacgaaggt gaaatctcct ggatagaagc ataataatac ccattgtcct ttaaaatctt
      481 ctaaacttac ttctgtaaac tcaccattat gataagctgg tgctgtaaac tgcggtgcag
      541 gtttacctac tttaatcatg ttttgttcct cctttttgat aattgttttt tcttctgtaa
      601 ctacttcttc acttgattta ctaatcttag gcctggcaca acctatctcc atctttttag
      661 tcataggacc ctccttctct attttaagtt aatatttgta atcgttacat atatgatacc
      721 atatcatatt ataaagtcaa cagctagtta ggttttgtta aatatttaac catccatctt
      781 cagtatatca gataatccat tcttttccag tccatataaa aaagacaggt ttaaacctgt
      841 cttttatttt accttatcca ttttttccct aactgttttt tctattagtt ttaccttctt
      901 ttcagccttc tcttctttct tatctttaga gtaggcatat aattttatct tcggttcggt
      961 gccggaaggc cttatggcat accaggaacc atcatctaaa aagtatttta aaacattgct
     1021 tttggccagc cctgtttcat catgtaagta gtctataact tttactagct tcatatctcc
     1081 aatagtcttt atgggagcct gtctaaattc ctccattatt ctcttaatcc ttctgcttcc
     1141 ttccatacct tctaaaacta tagatattaa tctttcctta tagtatccat attttttata
     1201 tatatcatct aaaaccttca gcaaatcttt accctttttc ttataatagg ctgccatttc
     1261 tgctattaac atagcactta ctatagcatc cttatcccta accaaggttc cataagtata
     1321 tcctatgctt tcttcatatc caaatatata atcatattca ccagtactct cccattcatt
     1381 ggccttggaa catataaatt taaagcctgt taaggtttca aacattttaa caccatgatc
     1441 ctcggctata gcagttccta aatcactggt tactatactt tttaccattg ctccattggc
     1501 cggtagatta ttatttctct ttcttgatga tagtatatag tgaaccagta gggctcctgt
     1561 ttggttacca tttaaaaagg taaattctcc tgccttgttt ttaaccataa tggctatcct
     1621 atcacaatct ggatctgtag ctattaatag atctgcatct ttcttttttc ccaacctaat
     1681 ggcatattca aaagctactg ggtcttcagg gttagggtat tctacagtgg taaaattaga
     1741 atcaggtagc tcttgttctg gcaccacata tatatttgta aaacctctgt cttttaagat
     1801 cctccttact ggaatattgc cagttccatt taaaggagta taaacaattt ttatatcctt
     1861 ttctatatct tcattgatgg cttccttaag ccttgtttca ttatattcat catctatctc
     1921 cttccctata tagcttataa gatttttctg tttagcttca gccagatcca taagctttat
     1981 atcctcataa tctctaattc tttctatttc tgctaagatc tcatcaccaa tttcatctaa
     2041 aatctgtgag ccttcttccc aataaacctt atatccgtta taatctttag gattatggct
     2101 agcagtaata actatacctg atatggtctt tagtcttctt actgtatagg aaagtagagg
     2161 agttgttcta ataccatcaa ataggtaaac ctttattcca ttggctgcca aaactctggc
     2221 tgctatctct gcaaatttgt ctgaatacct tcttacatca taggctatgg ctacaccctt
     2281 atccatggct tcttgcccct tatttattat ggtttgagca aggccttgag ttgccagggc
     2341 cacagtatat ttattcatcc tattagtacc agcccctatc ttacctctta gaccagc
//
LOCUS       DATGSI010000142         4185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_388777, whole genome shotgun sequence.
ACCESSION   DATGSI010000142 DATGSI010000000
VERSION     DATGSI010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388777"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2057
                     /locus_tag="VK031_02255"
     CDS             <1..2057
                     /locus_tag="VK031_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52766.1"
                     /translation="RGRSIEQIRAQYRKEAEEGIKAYHHNPKREQQAVNLYFNYDDNI
                     YAIDSNGNYVTLNPNVATEQDIQNSGVIKKQFYNEYLTNLQGVEKNTTFEEVYNPDVF
                     IGTEEQTPTQNIINNYYYNSEDTKEDKKETTEVEEAKKILDERQFLVGLIQAGAFDLQ
                     NPEYKRVPTMQDGGQIPTSSQGLYEYPNQTVKVPTKDGRITMKNIPYPVVGISAETGE
                     QILMFPEQEYQFKNTKNVIEIPLSQYSTNKNMQEGGNISYGTIVPILQADGSIKYENV
                     RGQGYTFANGGYINRFEDGGGIAPKRKGTFDKDAFKRGIAYVESRNFENPYEAIPIHK
                     GELLSSAAGKYQFLYNNIKDLPQMRGVSKEDFLNSPELQEMIMDLAIEGKLPDRPGYY
                     KNANDLTNSYAPLYGENWRYRPDEVAALTHYLGRKGARDYMRAQLEGRDYTVSGKTNM
                     DVEDYLRTYNEGAGVEYIPKTQSQTSQQPQDPYEERVSFTSGASYQQDPRVIRDMQSI
                     DRMNPNNYRRNIRLPNNMGRGEFPTYIPPPGGMESIEEPIGDYSSDPRPQSQISMTES
                     QTTKPQAQLTGTDFIQQYIQTNRDKFAYGGYVNGFEDGGEINHDGCGGPGQPPCEENS
                     FKIDYKPFTKYPKSALGNNGMFDMSNPNIYKSLVPAAIGAGALSQLEQKREDFKKALG
                     EK"
     gene            2116..>4185
                     /locus_tag="VK031_02260"
     CDS             2116..>4185
                     /locus_tag="VK031_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52767.1"
                     /translation="MAANNKQIETRVKQLMAMGIAYPQAVAIANSEIPTMLTGGGIDP
                     NYLQNLEAESYQLDKDYKDLQMQRELEWAQGLDAWNDNTMDEQLTKEFREHEKRLDQQ
                     DGLNLNTEELKKLFNPYAGYSMEDALMGMGRFVGGDRSVQNAMGFIKAPLAGARSFMS
                     GQGLARTEKYAQDEYDRRQREGLVGNYKQEGGRVTPEEIEKITGIRFSEEAANNLFER
                     VTQNPKDAVKRQIELGGYKGRNFFNIQDQGSNVRFVPTKENPQNYQGYKRQLEYIQKL
                     NPDIKIDNGAYLHFRQRQPYGMQDGGEVKTHTFEGTMGYARADEGIEETEEEILFRRF
                     MEYLENNKENLPTINRKTEDITPEEMQRIEEEKIIREHFYQTGGQLTFPERMTGEYIQ
                     GIPEDSPEEPIVELEDGEHILQPDGVMSEVKGKKHSQGGEKLTSDQIEEGTIVVSDHL
                     KIGKDNAKYFKDKYNIKVKASDTYATVVDKIKSKTGLTKLNKEQEKIIKEIQKQEKST
                     KDVATKNINLEFLSEKINELEEEKEIIEEVVREALIDVYNVQESEKPVEEQEPVVDTD
                     ILNQVAQQYDIPEEQAMGIVQEYLKGGKIPKMQGGSIYDIYGKDMIKTGQGIVQKFPE
                     EMPKKRARLTTHNPEHLMEMNLLLNPNISEQERERLLRTIDKNNNRRVLTNIQEEGTT
                     DTTQTTGT"
BASE COUNT         1733 a          635 c          857 g          960 t
ORIGIN      
        1 ctaggggtag gagcatagaa caaataagag cacagtatag aaaggaagct gaagaaggca
       61 taaaagcata tcaccataat cctaaaagag aacaacaggc tgtaaacctt tactttaatt
      121 acgatgacaa tatatatgca atagactcaa acgggaatta cgtaacactc aaccctaatg
      181 ttgcaacaga acaagatata caaaatagtg gagtaataaa aaaacaattt tacaacgaat
      241 acttaacaaa cttgcaagga gtagaaaaaa acactacttt tgaggaagta tataatcctg
      301 atgtatttat aggaaccgag gaacaaacac ctacacaaaa tattattaat aactactact
      361 acaacagtga agacacgaaa gaagataaga aagaaaccac agaagtggaa gaagcaaaaa
      421 aaattttaga cgaaaggcag tttttagtag gtcttataca agcaggagcc tttgatttac
      481 agaatcctga atataaaaga gtacctacta tgcaagatgg agggcaaata cctacatcaa
      541 gtcaaggact ctacgaatac ccaaaccaaa ctgtaaaagt tcctacaaaa gacggaagaa
      601 taacaatgaa aaacattcct tatccagtag taggaatatc cgcagagaca ggagaacaaa
      661 tattaatgtt ccctgaacaa gagtatcaat ttaaaaacac caaaaacgta atagaaatac
      721 cgttaagcca atatagcaca aacaaaaaca tgcaggaagg gggaaatata tcttacggga
      781 caattgttcc aatattacag gctgacggga gtataaaata tgaaaatgtt agaggacaag
      841 gttatacatt tgccaatggt ggctatataa ataggtttga ggatggaggt ggaatagctc
      901 ctaagcgcaa aggtactttt gacaaagatg cctttaaaag gggtattgca tatgttgaaa
      961 gtagaaattt tgaaaatcct tatgaggcta taccaatcca caagggtgag ctcttatcat
     1021 cagctgctgg taaatatcaa ttcctttata ataatataaa agatttacct caaatgagag
     1081 gtgtcagtaa ggaagatttc ttaaatagtc ctgaactaca agaaatgatt atggaccttg
     1141 caattgaagg caagcttcct gatagacctg gttattataa aaatgcaaat gacctcacta
     1201 attcctatgc accattatac ggtgagaact ggagatatag gcctgatgag gtagcagctc
     1261 ttacacacta tttaggtaga aagggtgcaa gggattatat gagagcccaa ttagaaggta
     1321 gggattatac tgtttcggga aaaaccaata tggatgttga ggattattta agaacctaca
     1381 acgagggtgc aggggtggaa tatattccta aaacacaatc tcaaacatcc caacaaccac
     1441 aagacccata tgaggaaaga gtaagtttta catcaggtgc ttcttatcaa caagatccta
     1501 gagttataag ggacatgcaa tctattgata ggatgaatcc caataattat aggcggaata
     1561 ttagattacc aaataacatg ggtaggggtg aatttcctac ctacatccct cctcctggtg
     1621 gtatggaaag tattgaagaa cctattgggg attattcaag tgaccctaga ccccaatccc
     1681 aaataagtat gactgagtca caaactacca agcctcaagc acagcttact ggtactgatt
     1741 ttatacaaca atacattcag accaatagag ataagtttgc ttatggggga tatgtaaatg
     1801 ggtttgagga tggtggggaa ataaatcatg atgggtgtgg aggtcctgga caaccacctt
     1861 gtgaagaaaa ctcctttaaa atagattata aacctttcac taaatatcca aaatcagcat
     1921 taggtaataa tggaatgttt gatatgtcta atcctaatat atacaaatca ttagtaccag
     1981 ctgcaatagg tgcaggagca ttatcacaat tagaacaaaa gagagaagat tttaaaaaag
     2041 cattaggcga aaaataacat attaaaaaat aaaactatga actcatagga atattatttg
     2101 aataaataaa ataaaatggc agcaaacaat aaacaaatag aaaccagagt aaaacaatta
     2161 atggctatgg gtatagctta cccccaagca gtggcaatag ctaactctga aataccaaca
     2221 atgctaactg gtggaggtat agatcctaac tatttacaaa acttagaggc tgaatcttat
     2281 caattagaca aggattataa agacctacaa atgcaacgag aattagagtg ggctcaaggt
     2341 ttggatgcgt ggaatgataa taccatggat gaacagttaa ctaaggaatt tagagagcat
     2401 gagaaaagac tcgaccaaca agacgggctc aatttaaaca cagaagaatt aaagaaactt
     2461 tttaatccct atgctggata cagtatggag gatgctttaa tgggtatggg caggtttgta
     2521 ggtggagaca ggtctgttca aaatgccatg gggttcataa aagccccttt ggctggagca
     2581 cggagcttca tgtctggaca agggttagct agaacagaaa aatacgcaca agatgaatat
     2641 gacagacgac aaagagaagg acttgttgga aattacaagc aagaaggggg aagagtgacc
     2701 ccggaagaaa tagaaaaaat aactggaata agattttcag aagaagcggc caataacctt
     2761 tttgaaagag taacacaaaa tccaaaggat gctgtaaaaa gacaaataga gttaggtgga
     2821 tacaagggca gaaacttttt caatattcaa gaccaaggat caaacgttag atttgtaccc
     2881 actaaagaga acccacaaaa ttatcaagga tataagagac aacttgaata catacagaaa
     2941 cttaaccctg atattaaaat agataatggg gcttatttac attttagaca aagacaacct
     3001 tatggtatgc aagatggggg agaagtaaag acccacactt ttgaaggtac tatggggtac
     3061 gcaagagctg atgaaggcat tgaggagacg gaagaggaaa ttctttttag gagattcatg
     3121 gaatacttag aaaataataa agagaattta cccactataa acaggaagac ggaagatata
     3181 acccccgaag aaatgcagag aatagaagaa gagaaaataa taagagaaca tttctatcaa
     3241 acagggggac aattaacttt tccagaaaga atgacagggg agtatataca aggaatacca
     3301 gaagacagtc cagaagaacc tattgtagaa cttgaagatg gggagcatat actacaacct
     3361 gacggagtta tgtcagaagt aaaaggaaag aaacacagtc aaggagggga aaagctaaca
     3421 agtgaccaaa tagaagaggg tacgattgta gtatctgacc acttgaaaat tggaaaagat
     3481 aatgctaaat attttaaaga taagtataac ataaaagtaa aagcaagcga cacttacgca
     3541 acagtagttg acaagataaa aagtaaaact ggacttacta aacttaataa agaacaagag
     3601 aaaatcatta aagaaataca gaagcaggaa aaaagcacta aagatgtagc aactaaaaat
     3661 ataaatttag aattcttgtc tgaaaaaata aatgagttag aagaggaaaa agaaataata
     3721 gaggaagtcg taagagaagc tttaatagat gtttataatg tacaggaatc agaaaaaccc
     3781 gtagaagaac aagaaccagt tgtagacaca gacattttaa accaagttgc acaacaatac
     3841 gatattccag aagagcaagc aatggggata gttcaagagt atttgaaagg aggtaaaata
     3901 cctaaaatgc aaggtggaag tatatatgat atatatggta aagatatgat aaagactggc
     3961 cagggaatag tacaaaaatt tccagaagaa atgccaaaaa agagagcaag gttaaccaca
     4021 cataacccag aacatcttat ggagatgaat ttgttactga accctaatat atctgaacaa
     4081 gaaagagaaa ggctattaag aaccatagat aaaaataata atagaagagt actaactaat
     4141 atacaagaag agggtacaac tgataccaca caaacaactg gtaca
//
LOCUS       DATGSI010000143         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_434067, whole genome shotgun sequence.
ACCESSION   DATGSI010000143 DATGSI010000000
VERSION     DATGSI010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434067"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VK031_02265"
     CDS             complement(<1..1286)
                     /locus_tag="VK031_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004050440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLS52768.1"
                     /translation="MPALKYHSPFQAVYFFFLNHNEHGNHHEHHEHNQHHEHHGHHGD
                     HEHHGGHDHHHHGNFKELFLKSLPLAIPILILSPMMGLTLPFQFTFQYSDILVGILST
                     ILLIYGGKPFYQGAIDEFKQKSPGMMALVSLGISVSYLYSIYAVIIRYTSGEHVMDFF
                     FEFASLVLIMLLGHWIEMKAVGDAGDAQKSLAELLPKDAHVVLEDDSIEIRSVTELVV
                     GDIVRVQAGENVPADGVIVRGESRVNEALLTGESKPVEKNIGDIVIGGSTNGEGILYI
                     KVQETGDKSFISQVQTLISQAQNQPSRAEGLANKVAGLLFYIAVIVAAIAFVIWLIIT
                     DLQTAIIFTVTTLVIACPHALGLAIPLVVARSTSLGASRGLLVKDREALELANKADVM
                     VLDKTGTLTTGEFKVLALDVISNKYSKDEVTALIAG"
     gene            1276..2163
                     /locus_tag="VK031_02270"
                     /pseudo
     CDS             1276..2163
                     /locus_tag="VK031_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611149.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2171..>2730)
                     /locus_tag="VK031_02275"
     CDS             complement(2171..>2730)
                     /locus_tag="VK031_02275"
                     /inference="COORDINATES: protein motif:HMM:NF021033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TM1802 family CRISPR-associated protein"
                     /protein_id="HLS52769.1"
                     /translation="RKEVLDRVQKGFEDYLREEKGLSASDIGLYTILIDNEPLSDLRE
                     YQNAVIESKQPKSKRRKKFRGVCSICGATENVSPDMTETKIKFYTTNQIIFGSDLSKN
                     NYYKNMQMCMDCLFRYLAGENYIMNKLQTKLADFNVYIIPQFVYGQPLNERDLDIATT
                     HIVDSFNMDWQPLFRQIYSEQEYTF"
BASE COUNT          933 a          455 c          455 g          887 t
ORIGIN      
        1 atgccagcga ttagtgcagt tacttcatct ttggaatatt tgttgctaat aacatcaaga
       61 gccaatactt taaattcacc agtggttaat gttccagttt tatctaagac cataacatca
      121 gctttatttg ctaattctaa agcttctctg tcttttacta ataagccacg acttgctcct
      181 aaacttgtac tacgtgctac tactagtgga atagctaaac ctaaggcatg tggacatgca
      241 ataactaaag ttgtcactgt aaaaatgatt gccgtctgta aatcagtaat aattagccaa
      301 ataacaaagg ctatcgcagc aactattact gctatataga ataacaagcc tgctacttta
      361 ttagcaagac cttctgctcg agatggttga ttttgtgctt gactgattaa tgtttgaact
      421 tgggatataa aagatttatc acctgtttct tgaaccttta tatataagat tccttcacca
      481 ttggttgatc caccaattac aatatcacca atattttttt caacaggttt ggattcacca
      541 gttaaaagtg cttcatttac acgtgattca cctcttacta taactccatc agctggcaca
      601 ttttctccag cttgaactct tacaatatct cctactacta actcagtaac tgaccgtatt
      661 tcgattgaat catcttctaa aacaacatga gcatccttag gaagtaattc tgctaatgac
      721 ttctgtgcat cacctgcatc tccgactgct ttcatttcaa tccaatgacc tagtagcata
      781 attaaaacca aagaagcaaa ttcaaagaaa aagtccataa catgttcccc tgatgtatat
      841 ctgataatta ctgcataaat actatataaa taagatactg atatacctaa agaaactaaa
      901 gccatcattc ccggggattt ttgtttgaat tcatctatag ctccctgata gaatggcttg
      961 ccaccgtaaa tcagcagtat tgttgataaa attcctacaa gaatatctga gtattggaat
     1021 gtaaactgaa atggcagtgt aagacccatc atcggagata atattaggat cggtatagcc
     1081 aatggtaatg attttaagaa tagctcttta aaattgccat gatgatggtg atcatgtcct
     1141 ccatggtgtt catggtctcc atgatgtcca tgatgctcat ggtgttgatt gtgttcgtga
     1201 tgttcatgat gattaccatg ttcattatga tttaagaaaa agaaataaac agcttgaaat
     1261 ggagaatgat attttaaagc aggcagcact actactaggc aaaaagtaga ccttatcatt
     1321 tctaatagag agaaatagag tattagtgca atgtgcagct tattaggggt ctcaagaagt
     1381 ttaatatatt atcatctaaa taaggaagaa gctgatgctt cagatgaaga atctaaaata
     1441 gaaaaacata taatagatat ctttcataaa agtagaaata actatgggac tagaaaaata
     1501 aaagttgagc ttgataaact aggttatcag gtttctagaa gaaggatagc cagaatcatg
     1561 aaagaaaatg ccttggtatc taactacaca gtagctcaat ataaagttca tagtaagggc
     1621 tgtaatgaat ctaatgtatc taatatagta gatagaaaat ttgatgatag agataggcta
     1681 gaggtagctg tgagcgacct tacctatgta atggtaggta ataaatggaa ttatgtatgc
     1741 acattggtag atctatctaa tagagaggta ataggctact cagctgggcc caataaggat
     1801 gctagtctaa tagaaaaagc actgttaagg tgcagatatt cactaaaaga tataaaggta
     1861 tttcactcag atagaggtag agagtatgac aatataaaaa tagataatat tttagaagcc
     1921 tttaatatag aactctcttt aagtagaaag ggaaatcctt atgacaatgc tgtaagtgaa
     1981 gcaatgaata aagttttaaa ggcagaattt atatatcaaa ataaatttga aagcttagat
     2041 gagctaaaat tacagcttgc agaatatatt tattggtata acaatataag aatccatggt
     2101 tctttaggtt atatatcccc agtagaatat agatatagag agagcttaga attagtagca
     2161 taagtgatat ttagaaagta tattcttgtt cggaataaat ttgtctaaaa aggggttgcc
     2221 aatccatatt aaacgaatca actatatgtg tagtagctat gtccaagtct ctttcattta
     2281 atggttgtcc ataaacaaat tggggaatta tataaacatt aaaatctgcc aatttagtct
     2341 gcaacttgtt cataatataa ttttcccccg cgagatacct gaataagcag tccatacaca
     2401 tttgcatatt tttgtaataa ttatttttag ataagtcgct cccaaatatt atctggtttg
     2461 ttgtataaaa ttttatttta gtttcagtca tatctggcga aacattctct gtagccccgc
     2521 aaatactaca tactcctcta aatttttttc ttctttttga cttaggttgc ttggactcaa
     2581 ttacagcatt ctgatactct ctaagatctg ataaaggttc attatcgatt aatattgtat
     2641 acagccctat atcagatgct gacaacccct tttcctctct caagtaatct tcaaatcctt
     2701 tttgtaccct atccaacact tcttttctta
//
LOCUS       DATGSI010000144         3030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_153500, whole genome shotgun sequence.
ACCESSION   DATGSI010000144 DATGSI010000000
VERSION     DATGSI010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153500"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /gene="rpsU"
                     /locus_tag="VK031_02280"
     CDS             <1..108
                     /gene="rpsU"
                     /locus_tag="VK031_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HLS52770.1"
                     /translation="ARSGIIGEARKRKYYEKPSVRRKKKAEAARKKRYR"
     gene            124..567
                     /locus_tag="VK031_02285"
     CDS             124..567
                     /locus_tag="VK031_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HLS52771.1"
                     /translation="MSLKNKLMEDLKLSMRNKDTIRKNTITMVRAAVKQKEVDERKEI
                     TDDEIIDIISKQFKEKRAAIDEFRKGNRNDLIEQTEKEMDILLEYLPEQLGEEEVEEI
                     VRETIEEVNATSMKDMGLVMKTVMPKVKGRTDGNIVSNIVKKILK"
     gene            691..1974
                     /locus_tag="VK031_02290"
     CDS             691..1974
                     /locus_tag="VK031_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HLS52772.1"
                     /translation="MKKSRLFLLLVIIFLLFQSFSIAESNEDVYVVPIKGEINRATKN
                     FVRDIVEEVDNKDAAAIIFEIDTNGGLIEEAIDIRDIILSTNVPTISYVNNKATSAGV
                     LITIASQHVVMAPNATIGSAEPIPNTEKILSFWRSILRDTAQYRDRNTDIIEAMADKD
                     IVVENVNKKGKLVNLTSKEAYDFKIADYVSDDYTDMLAYFGFDRANIIEKKEGLQIKL
                     AKHIANPYLSSILLTVAFIGMVVEILTPGFGIGGTVSIIGFGLYFGGNILAGNSHWTS
                     LGLFVTGLILLVIEGIVPGFGLPGISGIIFVLVGIILAMDSFQVALVSLSIAIIITTI
                     VTIILVKLGYRSKLFQSIILSTEHRGDKGYVSAKPMEEYEGKIGKAITGLRPSGFVEI
                     DGKRIDAISYGEYITKGESIKVVKVEGTKIFVRRV"
     gene            1978..2958
                     /gene="floA"
                     /locus_tag="VK031_02295"
     CDS             1978..2958
                     /gene="floA"
                     /locus_tag="VK031_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345632.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HLS52773.1"
                     /translation="MEFLFLGGVVLLIIIFLMLFFTFIPVGLWITAIFSGVKIGISNL
                     IGMRLRRVAPNRIVSPMIKATKAGLSVGVDQLEAHFLAGGNVNSVVDALIAAQRANIE
                     LEFERAAAIDLAGRNVLEAVQVSVNPKVIETPQISAVAKDGIEVIVKARVTVRANIER
                     LVGGAGEETIIARVGEGIVTTVGSADSHKDVLENPDSISKTVLDKGLDSGTAFEILSI
                     DIADVDVARNIGARLQMDQAEADKRIAEAKAEERRAMAVAKEQEMKADVQAMRARVVE
                     AEAEVPLALAAALKEGKMGVMDYYNMKNVLADTDMRSSISRLTEDDKKEK"
BASE COUNT         1073 a          359 c          707 g          891 t
ORIGIN      
        1 gcacgttctg gcattatagg cgaagctaga aaaagaaaat attacgaaaa gcctagtgtt
       61 agaagaaaga aaaaagcaga agcagcaagg aaaaaaagat atagataatt aagaggtgaa
      121 ggtatgtccc tgaaaaacaa attaatggaa gatttaaaac tctccatgag aaataaggat
      181 actattagga aaaatacaat aacaatggtt agggctgctg taaaacaaaa ggaagtagat
      241 gaaagaaaag aaatcactga tgatgaaata atagatatta tctccaagca atttaaggaa
      301 aaaagggctg ctattgatga gtttagaaaa ggcaaccgaa atgatttaat tgaacagact
      361 gaaaaagaaa tggatatcct tttggaatat cttcctgagc aattaggcga ggaagaagtg
      421 gaggaaattg ttcgtgaaac tattgaagaa gtaaatgcca cctccatgaa ggatatgggt
      481 cttgttatga aaactgttat gcctaaggta aaaggcagga cagatggaaa tatagtaagt
      541 aatatagtta agaaaatttt aaagtaacct ggtaagatcc aggttatttt tattctttaa
      601 ttgaaaaggg tattatatta atatagaata gaattaatta aggggctata taatattagt
      661 aaattgtagt tttgaaatgg aggtggcagt gtgaaaaagt ctagattatt tttattgttg
      721 gtaatcattt ttttattgtt tcaaagtttc agtattgcag agtctaatga agatgtgtat
      781 gttgtgccta ttaagggtga gattaatagg gccactaaaa actttgtaag agatatagtt
      841 gaggaagtag acaacaaaga tgcagcggct attatatttg aaatagacac taatggaggc
      901 ctgatagagg aagccattga tataagggat ataatcttaa gtacaaatgt acctaccata
      961 tcttatgtaa ataacaaggc aacttctgct ggagtcttaa taactatagc cagccagcat
     1021 gtggtaatgg ctccaaatgc taccattggt tcggcagaac ctatccctaa tactgagaag
     1081 attctatctt tttggagatc tattttaaga gatacagccc aatatagaga taggaataca
     1141 gatataatag aggctatggc agacaaggat atagttgtag aaaatgttaa taaaaagggt
     1201 aagctagtaa acttaacttc taaagaagcc tatgatttta aaattgccga ttatgtgtca
     1261 gatgattaca cagatatgtt ggcttatttt ggatttgata gggctaatat aattgaaaaa
     1321 aaggagggcc tacagataaa actggccaag catatagcca atccttattt aagcagtatc
     1381 ttattgactg tagcctttat aggaatggtt gttgaaattc taacaccagg ttttggtata
     1441 ggtggaactg taagtattat aggattcggc ctttactttg gtggaaacat tttggcaggt
     1501 aactctcact ggacctcttt aggtctattt gtaacgggtc tgattttatt ggttatagaa
     1561 ggtattgttc cagggtttgg tttacctgga atcagtggta ttatttttgt tttagttggg
     1621 attattcttg ctatggattc tttccaggta gctttggttt cattgagtat agccattatt
     1681 attaccacaa ttgtaacaat tatattggtt aaactaggtt atagaagcaa gctatttcaa
     1741 tctattatct tgtctacaga acatagggga gataagggtt atgtaagtgc aaagcctatg
     1801 gaggaatatg aaggtaaaat aggaaaagct attactggat tgcgaccttc tggctttgta
     1861 gaaatagatg gaaaaaggat agatgctatc tcctatgggg aatatattac caagggggaa
     1921 tctatcaagg tggtaaaagt agaaggaaca aaaatatttg taaggagggt ataatttatg
     1981 gaatttttat ttttaggtgg ggtagtgtta ttaattataa tatttctcat gctattcttt
     2041 acctttattc cagtaggcct atggattaca gccatatttt ctggggttaa aataggtata
     2101 tcaaacttaa taggaatgag gttaagaagg gttgctccta atagaatagt tagcccaatg
     2161 attaaggcaa caaaggcagg tttaagtgta ggggtagatc agttagaagc acatttttta
     2221 gctggaggta atgtaaattc ggtagttgat gctttaattg cagcccaaag agctaatatt
     2281 gagctagaat ttgagagagc agctgccatt gatttagcag gaagaaatgt attagaggct
     2341 gtacaggtaa gtgttaatcc taaagtaatt gaaacgccac agatatcagc agtggcaaag
     2401 gatggtattg aagttattgt aaaggctagg gtaactgtaa gagctaatat tgaaaggtta
     2461 gtaggagggg ctggtgagga gacaattata gccagggtag gagaaggtat tgtaaccaca
     2521 gtaggtagtg cagactctca caaggatgtc ttagagaatc cagatagtat atctaaaact
     2581 gtattagata aggggttaga ttctggaaca gcctttgaaa tcttatcaat agatatagct
     2641 gatgtagatg tggctagaaa tattggagct aggctacaaa tggatcaggc agaagcagat
     2701 aaacgtatag ctgaagctaa agcggaagaa agaagagcca tggcagtagc caaggaacaa
     2761 gaaatgaaag cagacgtaca ggcaatgagg gccagagttg tggaggcaga ggcagaggta
     2821 cctttagcat tggcagctgc cttaaaggaa gggaaaatgg gtgttatgga ttactataac
     2881 atgaaaaatg ttttggcaga tactgatatg agatcatcca tttctagatt gactgaagac
     2941 gacaagaaag aaaaatagga tgtgattagg tgccaacaat tatatattta attgcagtga
     3001 tttttttaaa tcttgtacta aaaagtatta
//
LOCUS       DATGSI010000145         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_291626, whole genome shotgun sequence.
ACCESSION   DATGSI010000145 DATGSI010000000
VERSION     DATGSI010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291626"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VK031_02300"
     CDS             <1..429
                     /locus_tag="VK031_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52774.1"
                     /translation="IYLIAVIFLNLVLKSIKDKKEIEKERRKRIEELRDTAKERQETY
                     TVLHRKEKKQERIYDVPIEEYKIDEEKTSYEESPISSVEPEKIVYLHDQAEKEEVKPR
                     QEVKKDQVKLDDLKKDLIRGIIVSEILSEPKSIKNIKRSM"
     gene            501..779
                     /gene="yqfC"
                     /locus_tag="VK031_02305"
     CDS             501..779
                     /gene="yqfC"
                     /locus_tag="VK031_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfC"
                     /protein_id="HLS52775.1"
                     /translation="MKKNVDNIKKTLTDALELPMDIALDLPKLVLMGDVRVDISNHKG
                     IIEYTSQLIRINTNIGVIKITGDSMEIKNILMEEISISGNIEKVEIHG"
     gene            790..1983
                     /gene="yqfD"
                     /locus_tag="VK031_02310"
     CDS             790..1983
                     /gene="yqfD"
                     /locus_tag="VK031_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HLS52776.1"
                     /translation="MLAIKIWNYLKGYVIIRIKGLSLERLLNLALVKDIYLWDVKRVS
                     NYEIEATVSLVGVKALDKLVERLGCQVKIVERRGLPFLLDRLRKRKMLGLGVIVFIIL
                     IGLLSSLVWQIEIIGTEQIPQMEILKLLEENNIKTGRFKKDIDKDQVERLILDKFQYI
                     SFLDIRKTGVKLIIELKEQDMPPEKADKSYPANVVAKKKGVILKIVAIKGKAVVNKGD
                     VVNPGDILISGIMDSELSDEVYLVHAEGEILAQARYTATVESPIIKYEERETGKTYKQ
                     RGIKFKDKGIKFFAGEIPFENYKEEVKEISLLRFDRLNIKLPIKLIYYTYRETELEEI
                     KQNIDFLKKSTQLEAIEKINKELSEESEILSKDSIFNIEDNILKTQVIIETIEDIGKV
                     QIISQ"
     gene            1996..2967
                     /locus_tag="VK031_02315"
     CDS             1996..2967
                     /locus_tag="VK031_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HLS52777.1"
                     /translation="MANRIGERSIGIENSQIVTEIFGKLDENINIIEKELNIDIVMRD
                     GSIKLIGEDANIELGERVIYHLRDVIKAEKVLTKQDVRYAIQMVAQGEEEKIKKLLND
                     VICITATGKGIKPKTLGQKRYIDSIRENDIVFGIGPAGTGKTYLAMAMAVQAFKNKEV
                     NRIILTRPAVEAGEHLGFLPGDLQEKVDPYLRPIYDALFEILGAENYIRYMEKGLIEV
                     APLAYMRGRTLDSAYVILDEAQNTTNEQMKMFLTRLGFGSKAIITGDITQVDLPRGKD
                     SGLKTVTKILENIDNIGIVQLSRLDIVRHPLVQKIIEAYEKFEEGNN"
     gene            3008..>3390
                     /gene="ybeY"
                     /locus_tag="VK031_02320"
     CDS             3008..>3390
                     /gene="ybeY"
                     /locus_tag="VK031_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HLS52778.1"
                     /translation="MIKIYFDNRQSIIDIDQEILNLLELVVEKSLEIEKYKGQYEVSI
                     SLVDNKEIKGLNQQYRGIDRETDVLSFPMGESFDLPIIPLGDIIISVEQAVKQAKEYN
                     HSLNRELAYLTGHSMFHLMGYDHEDV"
BASE COUNT         1302 a          411 c          733 g          944 t
ORIGIN      
        1 atatatttaa ttgcagtgat ttttttgaac cttgtactaa aaagtattaa ggataagaaa
       61 gaaattgaaa aggaaagaag gaaaagaata gaggagctta gagatactgc taaagaaaga
      121 caagaaacat atactgtctt gcataggaag gagaaaaagc aagagagaat ctatgatgtg
      181 cccatagaag aatacaaaat agatgaagaa aaaacttcct atgaggaaag tcctataagc
      241 tctgtagaac cggagaaaat agtatattta cacgatcaag cagaaaaaga agaggtaaaa
      301 cccaggcagg aagttaaaaa ggaccaagta aagctagatg atttaaagaa ggatttaatt
      361 agaggtatta ttgtttcaga aatcctatca gaaccaaaaa gtataaaaaa cattaaaaga
      421 agcatgtagc cttaggccat gcttctttcc ctttgtaata gtaattttat tttcagcata
      481 ttattagtag gagggatttc atgaaaaaaa atgtagataa tataaagaaa actcttactg
      541 atgccttaga attaccgatg gatattgccc tagatctgcc taagctggta cttatggggg
      601 atgttagggt tgatatttct aaccataagg gaataattga atacactagc caactaatta
      661 ggataaatac caatattggt gtgattaaga taaccggcga ttctatggaa attaaaaata
      721 ttcttatgga ggaaattagt atcagcggaa acattgaaaa agttgaaatt cacggctagg
      781 ggtgttaata tgctggcaat aaaaatatgg aattatttaa agggatatgt tattattagg
      841 ataaaaggtt taagtctaga aaggttgtta aatctagcct tagttaagga tatttacttg
      901 tgggacgtta agagggtaag taattatgaa attgaggcta ctgtatcttt agtgggggtg
      961 aaggccttag acaaactagt tgagaggcta ggctgtcagg ttaaaatagt tgaaagaagg
     1021 ggccttccat ttttgctaga taggttaagg aaaagaaaga tgctaggctt aggtgttata
     1081 gttttcatca tcctgatagg ccttctttca tccttggttt ggcagataga aatcataggt
     1141 acagagcaga ttccccaaat ggaaatctta aaacttttag aagagaacaa tataaaaaca
     1201 ggtaggttta aaaaagatat agacaaggac caggttgaga ggctgatttt agataaattt
     1261 caatatattt cttttttgga tattagaaaa actggagtta aactaataat tgaattaaag
     1321 gaacaggata tgccacctga aaaggcggat aaaagttacc ctgccaatgt ggtggccaag
     1381 aaaaaaggag taatcctaaa gattgttgct attaaaggta aggcagttgt caataaggga
     1441 gatgtggtta atccaggaga tattttaatt tctggaatta tggacagtga gctaagtgat
     1501 gaggtatact tagtccatgc agagggagaa attttagccc aagccaggta tactgccact
     1561 gtggaaagtc ccataattaa atatgaggaa agagagacag gtaagaccta taagcaaagg
     1621 ggcattaaat ttaaggataa ggggattaag ttttttgctg gagaaattcc ctttgaaaat
     1681 tataaagaag aagtaaagga gataagttta ctaaggtttg atagactaaa tattaaatta
     1741 cctataaaac ttatttatta tacatatagg gaaacagagt tggaagagat aaaacaaaat
     1801 atagactttt taaaaaagtc tacccaactg gaggctattg aaaagataaa caaggagcta
     1861 tctgaagaaa gtgagattct ttctaaggat tctattttta atatagaaga taatatatta
     1921 aagacccagg ttattataga aaccattgaa gatataggta aggttcaaat aatttcacaa
     1981 taggaggctt agaatttggc aaacagaatt ggagaaagaa gtattggcat agaaaacagt
     2041 caaattgtga ctgagatctt tggaaaatta gatgaaaata taaatattat agaaaaagag
     2101 ctaaatatag atattgtaat gagggatggc agtataaaat taattggtga agatgccaat
     2161 attgagctgg gagaaagagt aatttaccac ttaagggatg taattaaggc tgaaaaagtt
     2221 ttaactaaac aggatgtaag atatgctatc caaatggtgg cccagggaga agaagaaaaa
     2281 attaaaaagt tactaaatga tgttatttgt ataactgcca caggcaaagg tatcaaacct
     2341 aaaaccctag gccaaaaaag atatatagac tctatcagag aaaatgacat agtatttggt
     2401 ataggacctg caggtaccgg aaagacctat ctggccatgg ctatggctgt gcaggctttt
     2461 aaaaataagg aggtcaatag gatcattcta acaagaccag ctgttgaggc tggagaacat
     2521 ttaggtttct tacctggtga tctccaggaa aaggtagatc cttacctaag gcctatttat
     2581 gatgccttat ttgaaatatt aggtgcagaa aactatatta gatatatgga aaagggttta
     2641 attgaagtgg cccctttagc atacatgagg ggaagaacct tagattcagc ctatgtaatt
     2701 ttagatgagg ctcaaaatac gaccaatgaa cagatgaaaa tgtttttgac caggctaggc
     2761 tttgggtcaa aggctataat aacaggggac ataacccagg tagacctacc gcgaggtaaa
     2821 gattctggct taaaaactgt taccaaaata ttagaaaata ttgataatat aggcatagtt
     2881 caattatcta gactggatat agtaaggcac cctttagtac aaaaaataat tgaggcctat
     2941 gaaaagtttg aggaggggaa taattaacct cctttaaatt cctttataaa aaatatggag
     3001 agataaaatg attaagattt attttgataa taggcaatca ataattgata tagaccaaga
     3061 aatattaaat cttttagagc tggtggtaga aaagtccttg gaaattgaaa aatacaaagg
     3121 acaatacgag gtgtctatat cactggtaga caataaagaa ataaaaggtc taaaccagca
     3181 gtataggggt atagataggg aaacagatgt cttatctttt cctatgggtg agtcctttga
     3241 cctgcccata attcctttgg gagatattat tatctctgta gagcaagctg taaaacaggc
     3301 taaggaatat aatcatagcc taaatagaga attggcctat ttaacaggtc atagtatgtt
     3361 tcatttaatg ggatatgatc atgaggatgt
//
LOCUS       DATGSI010000146         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_291633, whole genome shotgun sequence.
ACCESSION   DATGSI010000146 DATGSI010000000
VERSION     DATGSI010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291633"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VK031_02325"
     CDS             complement(<1..915)
                     /locus_tag="VK031_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS52779.1"
                     /translation="MEKYIIEMKNITKTFPGVIANDNVTLQLKKGEIHALLGENGAGK
                     STLMSILFGLYQPDSGEIRKNGKPVQINNPNDANDLGIGMVHQHFQLVEIFTVLENII
                     LGVEPNTMGFLKRDEARDRVIELSERYGLRVDPDALIQDITVGMQQRVEILKMLYRDN
                     EILIFDEPTAVLTPQEIKELLQIMREFASEGKSILFITHKLNEIMEVADRCTVLQKGK
                     YMGTVHIKDTSKEELSRLMVGRDISFAVDKEEKKPGDVVLSVRNLTVKSKQHRAEVVK
                     NVSFDVRAGEIVCLAGIDGNGQKELVEAL"
     gene            complement(1079..2209)
                     /locus_tag="VK031_02330"
     CDS             complement(1079..2209)
                     /locus_tag="VK031_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HLS52780.1"
                     /translation="MKRFFSLMLVIALATVTLAGCGSQEYEIALITDKGNIDDKSFNQ
                     GSWEGVVEFAEAENISHNYIKPEEANDAGYLAAIDLAVEGGAKVVVTPGFLFEVAIHE
                     AQEKYPDVKFILLDGVPHAADSHEPDIAENTASVLYADEEAGYLAGYAAVKDGFTHLG
                     FMGGMAVPAVQAFGYGYLQGAEDAAAEMGLEDGAIKVIYHYTGDFDENDTNKATASAM
                     YQEGAEVIFGCGGAVGKSVMSAAAEADAKFIGVDVDQRYDSETTITSATKGLAASVKA
                     VLEAIYKTDSWEDYSGKTTVFNASNDGVGLPTTVIGDEDADAFDRFNTFDREQYDEVY
                     GRLKAGEIPVEREIDVADPSGNATAEELTTQLNLKKVVVTTR"
BASE COUNT          619 a          540 c          338 g          790 t
ORIGIN      
        1 caaggcctcc accaattcct tctgtccatt tccatcaatg cctgccaggc atactatttc
       61 tccagccctt acatcaaagg atacattttt tactacttct gccctgtgtt gtttagactt
      121 tacagtcagg tttctcactg acaataccac atcaccaggt tttttctcct ctttatctac
      181 tgcaaagcta atatcccggc ctaccattag ccttgaaagt tcttccttac tggtatcctt
      241 tatatggaca gtacccatat atttgccttt ttgaagtaca gtacacctat ctgccacttc
      301 cataatttca ttaagtttat gggtgataaa aaggatagat tttccttcac tggcaaattc
      361 cctcataatt tggagtagtt ccttaatttc ctgtggagtt aaaactgcag ttggttcatc
      421 aaatattaaa atctcattgt ccctataaag catctttaaa atttctaccc tttgctgcat
      481 acctacagtt atatcctgaa ttaaggcatc agggtctacc cgcagcccat acctttcact
      541 taattctatt accctatctc tagcctcatc cctttttaag aatcccatgg tattaggttc
      601 tactcctagg atgatatttt caagcacagt aaagatttct accagctgga aatgttggtg
      661 gaccattcca atacctaaat cattggcatc atttggatta ttgatttgaa caggtttacc
      721 gtttttccgt atttctccag aatcaggttg atataggccg aagagtatgc tcatcagggt
      781 agatttacct gctccgtttt cacctaataa agcatgaatt tctccttttt tcagttgtag
      841 ggtgacatta tcattggcaa tgacacccgg aaacgttttt gttatatttt tcatttcaat
      901 aatatatttt tccactttaa ccccatcttt tcattatttt tattaaacac agacattagc
      961 cttcacaaga atataaaaac tttgtgaaaa gttctaagtt aaaaaaatag gggaatttaa
     1021 gccttcccct atttttataa tcttacttac ataaggctta gcccaatgtt ggtaataatt
     1081 atctggtagt tactacaacc tttttaagat ttagttgtgt tgttaactct tcagcagttg
     1141 cattacctga aggatctgca acgtctattt ctctttcaac tgggatttct cctgccttaa
     1201 gtcttccata tacttcgtca tattgttctc tatcaaaagt attaaatcta tcgaaggcat
     1261 ctgcatcttc atcacctatt acagtggttg gaagtcctac accgtcatta ctagcattga
     1321 atactgtagt ctttccactg tagtcttccc agctatctgt tttatatatg gcttcaagta
     1381 ctgcttttac agatgcagcc aaccccttag ttgctgatgt aattgtagtc tcactgtcat
     1441 atctttggtc tacgtctaca ccaataaatt tagcatcagc ttctgctgct gcagacataa
     1501 cagacttacc tactgctcca ccacaaccaa agataacctc tgcaccttct tgatacatgg
     1561 cactggccgt agccttgttt gtatcatttt cgtcaaagtc accagtatag tggtaaataa
     1621 ccttaatagc tccatcttct aaacccattt ctgctgccgc atcttcagca ccttgtagat
     1681 agccatatcc aaaggcttga actgctggta cagccatacc acccataaaa cctaggtggg
     1741 tgaaaccgtc tttcacagct gcatatcccg ccaaatatcc tgcctcttca tctgcatata
     1801 aaacactggc tgtgttttct gcaatatcag gctcatgact atctgcagca tgaggtactc
     1861 catctaatag aataaacttc acatctgggt atttttcctg tgcctcatga atcgctactt
     1921 caaataggaa acctggtgta actactactt tagctccacc ttctactgcc aggtctatag
     1981 ctgccaagta ccctgcatca ttggcttcct caggcttaat gtagttatga gaaatgtttt
     2041 ctgcttcagc aaattctact acaccttccc atgaaccttg gttaaaagac ttgtcatcaa
     2101 tgtttccctt gtctgttatt aatgctattt catattcttg ggaaccacaa ccggccaagg
     2161 ttacagtagc caaagctatg accagcatta acgagaaaaa tcttttcata ttagaacccc
     2221 ctagtttatt tattcaattt aattatatgc tatttatctg ttaatttcaa tacaaagacg
     2281 cttccca
//
LOCUS       DATGSI010000147         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_153780, whole genome shotgun sequence.
ACCESSION   DATGSI010000147 DATGSI010000000
VERSION     DATGSI010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153780"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VK031_02335"
     CDS             <1..212
                     /locus_tag="VK031_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HLS52781.1"
                     /translation="LKKDDDLGIDPDPEKPGDEDSKPEPEKPSKDEDRKLPATGEKRS
                     LLPLISIPLFVVAALIFLKRKRINS"
     gene            295..867
                     /locus_tag="VK031_02340"
     CDS             295..867
                     /locus_tag="VK031_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52782.1"
                     /translation="MIRIKKIYMIIAFIILITFFKGCTEPADTNIPERIYSTDESKKT
                     NDGSKTDPVEEDCEDNQIGTVRNTREGEVLFNQDDIIITLESINLDKMYLPFVQLNIE
                     NISDKRILVEIEDVSINGVMVTNDPKFACEVTPSDIMEDSIMFSEQELEYAEISTVQE
                     IEFSFRITDVETSETIAKSDSIMLEVAENP"
     gene            928..1242
                     /locus_tag="VK031_02345"
     CDS             928..1242
                     /locus_tag="VK031_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52783.1"
                     /translation="MMKREFTYEVPWKSDIYMYIENNSQEHIIINVENIAVNEFIISS
                     DFATNVLPGKKRYESFKFTKNDFEDHTIESIKNMTFNLKISKDTSSDTILSISKIKVD
                     FE"
     gene            1425..2063
                     /locus_tag="VK031_02350"
     CDS             1425..2063
                     /locus_tag="VK031_02350"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HLS52784.1"
                     /translation="MKIIDLSQTFENGMPQFPNTKPIKIEQIAQIREGGFRVTDLQST
                     VHNGTHCDAPAHCIEGGKTIDQLSLDTFVGEAIMLDADVEEKREISTEILEGVEIQPK
                     DIVIIRTGYSKYWGQERYVYDAPYLSEELAQSLSNLDIKSIGIDFISPDKVDSIRSSV
                     HNILMKENIPFIENLNNLDKIKKERFFFSAAPLLIYKSDGGLARAFAVIFDE"
     gene            complement(2113..>2837)
                     /locus_tag="VK031_02355"
     CDS             complement(2113..>2837)
                     /locus_tag="VK031_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HLS52785.1"
                     /translation="ILLIIMASKKHTIRLDLAKGFRFNRAIIENLIRIGGPAAIEQVS
                     FRVGVVFFIKIVSGLGTVVYATHQIALNILSLSFAPGQAFGIAASTLVGRSLGEERID
                     RADAFIKESNRLAIFSALFFGAIFFFFGPRLTALYTEDTSVIEESVNIMRIMAFIQPF
                     QGSAFAFSGGLRGAGDTVSTLIVTMLGVVVVRLSVAYILINIVGIGLIGAWIAMFLDQ
                     FVRWLGIVIRYRTGKWKYIKLK"
BASE COUNT         1072 a          447 c          525 g          793 t
ORIGIN      
        1 tattaaagaa agatgacgat ctaggaattg atccagaccc agaaaaacca ggagacgaag
       61 actctaaacc agaacctgag aaaccaagca aggacgaaga tagaaaacta ccagcaacgg
      121 gagaaaaacg tagcctattg ccactaattt ctataccatt atttgtcgta gcagcactaa
      181 tattcttaaa aagaaaaaga ataaatagtt agaatttaga tcctccaaag cctgcttttc
      241 ataaggcagg ctttgggttt attatgtaag ttttggaaat tagagggtga tagattgatt
      301 agaatcaaaa agatatatat gataatagct tttattatat tgataacatt tttcaaagga
      361 tgcactgagc cggcagatac aaatattcca gagagaatat atagcacaga tgaatctaaa
      421 aaaactaatg acggtagtaa gacagatcct gtagaagagg actgcgaaga caatcaaata
      481 ggtacggtaa ggaacaccag agagggtgaa gttttattta atcaggatga tataatcatt
      541 actcttgaat ctataaattt agataaaatg tatcttccct tcgtacaact aaatatagaa
      601 aatatctctg ataaaagaat attggtagag atagaggatg tatctattaa cggggtaatg
      661 gttactaacg acccgaaatt tgcttgcgag gtaactccaa gtgatataat ggaagatagc
      721 ataatgtttt cggaacaaga attagagtat gcggaaatat caactgtaca agaaatcgag
      781 ttttcatttc gtataacaga tgtagagact agtgaaacaa tcgctaaatc agactctatt
      841 atgcttgagg tagcagaaaa tccttgatat atacaaaact atgatgatag tgggattgaa
      901 gttttcaatc aagaaggtat tagaattgtg atgaagaggg aattcacata tgaagtacct
      961 tggaagtcag atatttacat gtatatagaa aataacagtc aagaacatat aattattaat
     1021 gtagaaaata tagcagttaa tgaatttatc attagttcgg attttgcaac aaatgtctta
     1081 ccaggtaaga agagatatga gagtttcaaa tttacaaaaa atgattttga ggatcacaca
     1141 attgaatcga taaaaaatat gacatttaat ctgaaaatat caaaagatac aagctcagat
     1201 actattttat ctatatccaa gataaaggtg gatttcgaat aactttgttt ttggggaaag
     1261 gagaatttgg ttaaaaatcc aagaaaattt atattagatt attacacaac cgaacgggct
     1321 cttcaaagaa ggttgtggca taaactctga tattcaacaa ttagtgtttg tagatttata
     1381 aagggattag gtacaattac attaaagtgt gaaaggggga tattatgaaa attatagacc
     1441 tatcacaaac tttcgaaaac ggcatgccac aatttccaaa cacaaaacct attaaaattg
     1501 agcagatagc tcaaataagg gagggaggat ttagggttac agatctgcaa tccacagtcc
     1561 ataatgggac ccattgcgat gcaccggccc attgtattga gggaggaaaa actattgatc
     1621 aactatcttt agacaccttt gtaggagaag ctatcatgct agacgcagat gtggaggaga
     1681 aaagagaaat ttctacagaa attctggaag gcgtagagat tcagcctaaa gatatagtaa
     1741 taataagaac aggatattct aaatactggg gtcaagaaag atatgtatat gatgctccat
     1801 acctatcaga agaactggcc caaagtttaa gtaacttaga tataaagtct ataggtatag
     1861 attttatatc tccagataag gtagatagca taagatcatc tgttcacaac atacttatga
     1921 aggaaaatat tccctttata gaaaacttaa ataatttaga taagataaaa aaggaaagat
     1981 tctttttttc tgcagcaccc ttattaattt acaaatcaga tgggggactg gccagggcct
     2041 ttgcagttat atttgacgaa taaaagttag aaaaagtgga ggttaaattc ctccactttt
     2101 tattttgata agctatttca actttatata cttccattta ccagttctat accttataac
     2161 tatacctaac catctgacaa actggtctaa aaacatggca atccaggccc ctattaagcc
     2221 gattcctact atattgatta aaatatatgc tacactaagc ctaactacta ccacaccaag
     2281 catggtaaca ataagggtag aaactgtatc tccagctccc cttaggccac cagaaaaggc
     2341 aaaggcagag ccctggaagg gctggataaa ggccatgatt ctcataatat taacagattc
     2401 ctctataaca gatgtatctt ctgtataaag agctgttaac cttgggccaa agaaaaagaa
     2461 aatagccccg aaaaataaag cagaaaaaat agccaatcta ttagattctt ttataaaggc
     2521 atcggcccta tctatccgtt cttcacctaa ggatctacca actagggtag atgctgctat
     2581 accaaaggcc tgaccaggag caaaggatag gctaaggata tttagggcaa tttggtgggt
     2641 tgcataaaca actgtaccta gacctgatac aatttttata aaaaatacaa ctcctaccct
     2701 aaaagaaact tgctcaatgg cagctggtcc acctattcta attaaattct caataatagc
     2761 cctgttaaat ctaaaacctt tggcaaggtc taatctaatg gtatgctttt tacttgccat
     2821 gattattaaa agtatta
//
LOCUS       DATGSI010000148         1181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_56945, whole genome shotgun sequence.
ACCESSION   DATGSI010000148 DATGSI010000000
VERSION     DATGSI010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56945"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1181)
                     /locus_tag="VK031_02360"
     CDS             complement(<1..>1181)
                     /locus_tag="VK031_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HLS52786.1"
                     /translation="STFVAFLFAYADAFVNIRFKKLFNMVSILPIISPPFALGMSFIM
                     LFGQRGIITHKLLGMQNSNIYGFRGLLAVQVLAFFPTAYLLLLGVLKQIDPSIDEASR
                     NLGASRWEVFKTVVLPLSKPGLLNAFLLVFIQSVADFGNVMVIGGNYNTLSAQVYMQS
                     MGNYDLKTGTALASVLLSISITMFIIQKYWLGSKSYITVTGKPSRKRVPIDDKHIKYP
                     IQILCTLIASFIIVLYALIPIGSIVRVWGVDYSLTLDHYIYIYKMGSKFIIDTTKISL
                     IATPITGILAMIISFLIVRKEFIGKKAIEFISLLAMAIPGTVVGMGYVLAFNTYPIML
                     TGTATIIILTFIVRNMPTGIRSGIVSLQQIDPAIEEAAQDLGASSFKVFTSITIPLIK
                     S"
BASE COUNT          438 a          205 c          168 g          370 t
ORIGIN      
        1 agactttatc aagggtatag ttatagaagt aaaaacctta aaagaactcg cccccaaatc
       61 ctgtgctgcc tcttctatag caggatctat ttgctgtaaa gatacaattc ccgatctaat
      121 tccagtgggc atatttctta caatgaaagt caatattatt atagttgctg tccctgtcaa
      181 cataatggga taagtattaa atgccaatac atatcccatt cctacaacag ttcccggtat
      241 tgccattgcc aacaaagata taaattctat agcctttttc cctataaatt cttttctaac
      301 aattaaaaaa gaaataatca tagccaatat cccggtaatt ggagtagcta ttaaggatat
      361 tttagtagta tctattataa acttggaacc cattttgtat atatatatat agtgatcgag
      421 agttaaggaa taatctacac cccatactct aactattgag cctataggaa ttaatgcata
      481 aagcactatt ataaaagatg caattaaagt acaaagtatt tgtataggat atttaatatg
      541 tttatcatct attggtactc ttttcctaga aggcttacca gttacagtaa tataggattt
      601 ggatcccagc caatattttt gaataataaa catagttata gatatgctta aaagtacaga
      661 tgctaatgca gtacctgtct ttaagtcata gttccccatg gattgcatat atacctgtgc
      721 agataaggta ttgtaatttc caccaattac cataacatta ccaaaatctg caacactttg
      781 aataaatacc agtaaaaagg catttaataa tccaggcttt gaaaggggaa ggactacagt
      841 tttaaaaact tcccatctag aagcacctaa atttctagat gcttcatcta ttgaaggatc
      901 tatttgtttt aaaacaccta gtaataataa atatgccgta gggaaaaatg ccaaaacttg
      961 aacagccaac aatcccctaa atccatatat attggagttt tgcatcccca ataacttatg
     1021 ggtaataatt cctctttgtc caaataacat aataaatgac atacctaagg caaaaggtgg
     1081 agatataata ggtaaaatag acaccatatt aaataatttt ttaaatctta tatttacaaa
     1141 agcatctgca taagcaaata aaaaggctac aaaagtagat a
//
LOCUS       DATGSI010000149         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_57084, whole genome shotgun sequence.
ACCESSION   DATGSI010000149 DATGSI010000000
VERSION     DATGSI010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57084"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1021)
                     /locus_tag="VK031_02365"
     CDS             complement(65..1021)
                     /locus_tag="VK031_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLS52787.1"
                     /translation="MDRNKKTIISIIGLIIFYLLVKVLINVGIIDSYIQLNLVIIGVN
                     IILAIGLNLITGFTGQFSLGHAAFMSIGAYTSAIITAKLGYPFIVALLVSGLMAALAG
                     ILIGIPTLRLKGDYLAIATLGFGEIIRIVALNTEYIGGAVGFNDIPRYTNWEWLYIMT
                     VITILLAYRFVNSSHGRACIAISQDELAAEAMGVNTTYYKVLIFAIGAFFAGIAGSLH
                     ANYFHFIKPDSFGFMKSVDILVIVVFGGLGSIAGSVVGAIALSIISLFLQGIPELRMV
                     FYAIILFLIMVYRPSGLLGKEEFKLFKKGGSLIDNFRGKKSV"
     gene            complement(1033..>1517)
                     /locus_tag="VK031_02370"
     CDS             complement(1033..>1517)
                     /locus_tag="VK031_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLS52788.1"
                     /translation="EKVFLDMKNIYIVLITLSLMIILQFVIQRTKIGKAMRAVSLDKE
                     AAELMGINVDRTISYTFAIGSALAGAAGVLVGIYYNTINPLMGSVPGLKAFIAAVLGG
                     IGIIPGAVFGGFFLGMTETVVSAYGGSVFKDAVAFSILIVVLLVKPNGLLGKTVREKV
                     "
BASE COUNT          505 a          334 c          215 g          463 t
ORIGIN      
        1 ggctatttca ccttctttta tacttaggtt tatatttctt actgccttaa ttccaccaaa
       61 attcttagac agatttctta cctctaaaat tgtcaattaa gctccctccc tttttgaata
      121 atttaaactc ttcttttcct aaaagaccag acggcctata caccataatt aagaataaga
      181 ttattgcata aaataccatt ctaagttcag gaatcccctg taaaaacagt gatataatcg
      241 atagggctat ggctcctaca acagaaccag ctatactacc taaccctcca aaaactacta
      301 tcaccaaaat atcaactgat ttcataaatc caaaggaatc tggttttata aaatgaaaat
      361 aatttgcatg taaggaacct gcaatgccag caaaaaaagc acctatggca aagattaata
      421 ccttataata ggtggtatta acacccatgg cctctgctgc caattcatcc tggcttatgg
      481 ctatacaagc cctgccatgg gaagaattaa caaatctata ggccagcagt atagtaatta
      541 cagtcattat atacagccat tcccagttag tatatcttgg gatgtcgtta aagcctacag
      601 caccacctat atactcagta tttaaggcaa ctattctgat tatttctcca aatcctaggg
      661 ttgctatagc tagataatct ccctttaacc ttaatgtagg aatccctatt aaaattcctg
      721 ccaaggctgc cattaaaccg gaaactagga gggccacaat aaagggatag cctaatttag
      781 cagttataat agctgaggta taggctccta tggacataaa agctgcgtgc cctagggaaa
      841 attgtccagt aaagcctgtt atcaggttta atcctatggc taaaattatg ttaaccccaa
      901 taattactag atttaattgt atatatgaat ctattattcc aacattgata agtaccttta
      961 caagcaggta aaatataatt aatcctatta tacttataat tgttttttta tttctatcca
     1021 taaataacac ctctacacct tttcccttac agttttccct aacagtccat ttggcttaac
     1081 taataaaact actattaaaa tagaaaaggc tactgcatcc ttgaataccg aaccaccata
     1141 ggcactaact acagtttcag tcatacctag gaaaaaaccg ccaaatactg ctcctggtat
     1201 aattccaatc cctcccagta ctgcagcaat aaaggcctta aggcctggta cagagcccat
     1261 caagggattt atagtattat aatagattcc taccaaaact cctgctgccc cagccagtgc
     1321 tgaacctatg gcaaaggtat aagagatggt cctatctaca ttaattccca ttagttcggc
     1381 agcctcctta tccaaagaaa ctgccctcat ggccttgcct atttttgttc tttggatcac
     1441 aaattgcagt attatcatta gacttaaagt aatcaggaca atataaatgt ttttcatatc
     1501 taaaaagact ttttcac
//
LOCUS       DATGSI010000150         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_106389, whole genome shotgun sequence.
ACCESSION   DATGSI010000150 DATGSI010000000
VERSION     DATGSI010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106389"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VK031_02375"
     CDS             <1..569
                     /locus_tag="VK031_02375"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HLS52789.1"
                     /translation="IFASLTNTEKYAMLLWSEKLKVIHASTHVSLRNACDLVKKDRIV
                     DTIRLANESMEKLGIKKPRIAVAGLNPHAGESGIFGDEEIKEIEPAIVETKKLGIDVE
                     GPIAPDTVFLRAYRGQYDIVVAMYHDQGHIPMKIIDFDSGVNITVGLPIIRTSVDHGT
                     AFGKAGKGTASELSMISAIKVADMYKIK"
     gene            686..1180
                     /locus_tag="VK031_02380"
     CDS             686..1180
                     /locus_tag="VK031_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HLS52790.1"
                     /translation="MSQENLNKTTTINRELVFVGLEAKDREEVLVKMGNNLEEQGFVH
                     DSYTEAIIKREKEFPTAIPTASMGIAIPHTDAEHVKKAGISIATLKEPVTFKNMGTGE
                     DMDVEIIFMLAVKDPNEQLNMLQRLMTIFQDESVLEKIKDAEKDEILDIVKSLLGDAV
                     VVDI"
     gene            complement(1221..>1717)
                     /locus_tag="VK031_02385"
     CDS             complement(1221..>1717)
                     /locus_tag="VK031_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLS52791.1"
                     /translation="YSPYYKVLDLDGNVAPGEQTCMGLGVMGAVGAKLAFPEKNVVCI
                     TGDGAFQMYNQDVPTAVQYDTPITWVILNSFSLEWPKIGQKELGGRYIATDFKAQPDF
                     AQLAQSYGCYGEQVESLSNLRPALKRALDANKSGKSAILDIIIDPSELPKGFLEYRLA
                     RSKD"
BASE COUNT          600 a          270 c          356 g          491 t
ORIGIN      
        1 aaatatttgc aagcttaacc aataccgaaa agtatgcaat gttactttgg agtgaaaaat
       61 taaaggtgat tcatgcatca acccatgttt ccttaagaaa tgcctgtgac ctggtcaaaa
      121 aagacaggat agtggatact ataaggttgg ccaatgaaag tatggaaaaa ctaggtataa
      181 agaaacctag aattgctgtg gctggactaa accctcatgc aggagaatca ggtatatttg
      241 gtgatgaaga aataaaagaa atagaacctg ctatagtaga gacaaaaaaa ttgggtatag
      301 atgtggaggg acctattgca ccagatacag tatttttaag agcctatagg ggccagtatg
      361 atattgttgt tgccatgtat catgaccagg gccatattcc gatgaagatc atagattttg
      421 attcaggggt aaatataact gtaggcttac ctatcataag gacttctgtt gatcatggaa
      481 cagcctttgg aaaagctggc aagggtacag ccagtgagtt aagtatgatt agcgccatta
      541 aggtggcaga tatgtataaa ataaaataat ctattttata taaaagtctt gtaaaaaata
      601 gattgaaata taattgttta ttatctagac ggtgtattta ataggtagtt tatgtactaa
      661 aactaaaaat taaggaggca tatagatgag ccaagaaaac ttaaataaaa ctacaactat
      721 aaatagagaa ttagtctttg taggtcttga ggctaaggac agggaagaag ttttagtaaa
      781 gatgggaaat aatcttgagg aacaaggctt tgtacatgat agctatacag aagccattat
      841 aaaaagagaa aaagagtttc cgacagccat ccctacagct agcatgggga ttgcaattcc
      901 tcacacagat gcagaacatg ttaaaaaggc aggaatctct attgcaactt tgaaggagcc
      961 tgttacattt aagaatatgg gaacaggtga agatatggat gtggagatca tatttatgtt
     1021 ggcagttaag gatcctaatg aacagttaaa tatgctgcaa agattgatga ccatattcca
     1081 agatgaaagt gttttagaaa agattaaaga tgcagaaaaa gatgaaatac tggatatagt
     1141 gaaatctcta ttgggagatg cagtagtagt agatatataa aaataaaaag aacatgccgg
     1201 aagggcatgt tctttttatt ttagtctttt gatctggcca gcctatattc taaaaatccc
     1261 ttaggaagct cactaggatc aataattata tccaatatgg cagattttcc ggacttgtta
     1321 gcatctaaag ctctttttaa agcaggtctt aaattactca gactttcaac ctgctcacca
     1381 tagcaaccat aggactgggc cagttgggca aaatcaggtt gggccttaaa atctgttgca
     1441 atatatcttc cgcccagttc cttttggcca attttaggcc attctaggct aaaggagttt
     1501 agtattaccc aggtaatagg tgtatcatat tgaacagcag taggtacatc ctgattatac
     1561 atttggaagg ccccatctcc tgttatacat actacattct tttcaggaaa ggccaattta
     1621 gcccctactg ctcccattac acctaacccc atacaagttt gttcgcctgg tgctacattg
     1681 ccatctaaat ctaaaacctt ataataaggt gagtagt
//
LOCUS       DATGSI010000151         4678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_11141, whole genome shotgun sequence.
ACCESSION   DATGSI010000151 DATGSI010000000
VERSION     DATGSI010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11141"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1515)
                     /gene="spoIVA"
                     /locus_tag="VK031_02390"
     CDS             complement(37..1515)
                     /gene="spoIVA"
                     /locus_tag="VK031_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HLS52792.1"
                     /translation="MQYFDIYKDIAERTDGDIYAGIVGPVRTGKSTFIKRFMELLVLP
                     NIEDYHKRERTKDELPLSGSGKTITTTEPKFVPNEAVELVLKDNVKFKVRMVDCVGYL
                     VEGALGHEEDETPRMVNTPWFDEAIPFQEAAEIGTEKVIKDHSTIGFVVTTDGSITDI
                     DRANYIQAEERVINELKELGKAFIIILNTKHPYLDSTLALKESLEEKYGVSVVVMDCL
                     NMNVGDVEKIFEKLLYEFPVKEITITLPGWVEGLARDHWIRNEIMDVLKPAIMDLQKL
                     NQVDDCLYPLEELDSVKDIRIDEINLGEGVVHTELITEEELFYKILNEITGYEIQGDY
                     QILSMINNLAEVKKEYDKIEKALIEAREIGYGLVSPSLEELELEEPEIYKQGNRYGVK
                     LRAKAPSLHVLRADITTEVAPLIGSEKQSEELVQYFLSEFEGEPAKIWQSNLFGKSLY
                     DLVSEQLNGKLTTMPEDARQKLRRALEKILNDGGGGLICIII"
     gene            complement(1621..2649)
                     /locus_tag="VK031_02395"
     CDS             complement(1621..2649)
                     /locus_tag="VK031_02395"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HLS52793.1"
                     /translation="MKAKIGIIGGGSWGTALGILLANKNHKVKMWVRNQGQLKEMQEK
                     SENVRYLPEVQLPKELKLSNNIEEVLSDKDIVLLATPSHSIRQILKEYGSHIKREQII
                     VNVAKGIENDTLMRVSQIVQEFLPDNKYVMLSGPSHAEEVARGIPTTVVSASTDRDAA
                     QYIQDVFMTVDFRVYTNLDVVGVELAGALKNVIALAAGISDGSGYGDNTKAALMTRGI
                     FEMARLGEKMGASPSTFSGLAGIGDLIVTCTSMLSRNRQAGILIGKGASVEESIREIG
                     MVVEGIKTTKSTYMLAKKYDVDMPITEELYKVLYEGKDVKQSVPNLMDRDKKEEMENM
                     VFFKGINW"
     gene            complement(2651..3241)
                     /gene="plsY"
                     /locus_tag="VK031_02400"
     CDS             complement(2651..3241)
                     /gene="plsY"
                     /locus_tag="VK031_02400"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HLS52794.1"
                     /translation="MKLLQIILISYLIGCFSSAYFIGKIFKDIDIRSHGSGNVGATNA
                     LRVMGLRMGILTFVFDILKGLISVIIGRSLMGYDGGLIAGLFAVVGHNWPIFIGFRGG
                     KGVATSLGVLLMTDWRLALVGVVLGLAVVLMTRYVSLGSVLFLSSQPFLYFLLDKTSN
                     RWALSLTILLALLAIIRHRSNFKRLAEGTENKIGGW"
     gene            complement(3241..4560)
                     /gene="der"
                     /locus_tag="VK031_02405"
     CDS             complement(3241..4560)
                     /gene="der"
                     /locus_tag="VK031_02405"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HLS52795.1"
                     /translation="MRKAVVCIVGRPNVGKSTLFNKLVGRRIAITEDTPGVTRDRIYA
                     EGEWLNHHFTVIDTGGLEPDNEDQIMSNIKRQAQMAIDTADVILFVVDGREGLTSTDK
                     EIGNLLRRSGKNVILVCNKLDSHRHWDRLYEFYELGFGEPMGISSEQSLGIGDLLDEV
                     IKLFPETVDTSLDEDQMRVAFIGKPNVGKSSLINYILGEERTIVTDIPGTTRDAIDSK
                     YEFEGNKYVFIDTAGLRRKRSIYEKVERYSVVRTLTAIDRSDLCVLVIDATEGVTEQD
                     TKIAGYAHENGKAIIIAVNKWDLVEKETMTYREFEIDVRTKLSFIHYAPLVFISALTG
                     QRVKKLLELINIVDNNYNLRISTGVLNDILNQAILMNQPPSDKGRRVKIYYGTQVSVR
                     PPKFVIFVRNKELMHFSYERYLENQIRGHFGFAGTPILFEFREKGAQ"
BASE COUNT         1338 a         1085 c          627 g         1628 t
ORIGIN      
        1 ctccctttta actaaatact tatttaaaaa ctttttttaa attattatac agatcaatcc
       61 accgccacca tcatttaaaa tcttttctaa ggcccttctt agcttctgcc tggcgtcttc
      121 aggcatagta gttagcttac catttaactg ttcgcttacc aggtcatata aggatttacc
      181 aaacaagttt gattgccaga tttttgcggg ctctccctca aattctgaaa ggaaatattg
      241 aaccagttct tcagattgtt tttcagaacc aataagagga gccacttctg tggtaatatc
      301 ggcccttaaa acatgcagag atggggcttt ggctctaagt ttaactccgt atctgtttcc
      361 ttgtttatat atctctggct cttccaattc taactcttct aaactagggc ttactaaacc
      421 atagcctatt tctcttgctt caatcaatgc cttttctatc ttatcatatt cctttttaac
      481 ctcagctaga ttatttatca tgcttaatat ttgatagtcg ccttgaattt cataacctgt
      541 aatctcattt aaaatcttat aaaataattc ttcctcagtt attagctctg tgtgtactac
      601 accttctcct aaatttatct catctatcct tatatcctta actgaatcta attcttctaa
      661 aggatacaaa caatcatcta cctggttcaa tttttggaga tccataattg ctggctttaa
      721 aacatccatt atttcattcc taatccaatg gtctcttgcc aatccctcaa cccaacctgg
      781 taaggttata gttatttctt taactgggaa ctcgtatagc aatttttcaa atattttttc
      841 tacgtctcct acattcatat ttaagcaatc cattaccact acagataccc catatttttc
      901 ctctaagctt tcctttaggg ctagggttga atccaagtat ggatgtttgg tatttaggat
      961 tattataaag gcctttccta gttcctttag ttcattaatt accctttctt cagcctgaat
     1021 ataatttgct ctatctatat ctgttattga accgtctgtg gtgacaacaa aaccaatggt
     1081 tgaatgatcc tttattacct tttccgttcc aatttcagcc gcctcttgga agggaatagc
     1141 ctcatcaaac cagggagtat taaccattct aggtgtttcg tcttcctcat ggcctagggc
     1201 accctcaact aagtagccta cacaatctac cattctaacc ttaaatttta cattatcctt
     1261 taaaaccaat tccactgcct cattgggaac aaatttaggc tcggtagttg taattgtttt
     1321 tcctgagcca cttaaaggta actcatcctt agttctttct cttttatgat aatcctcaat
     1381 attgggtaac acaagtaatt ccataaatct ttttataaag gttgacttac cagttctaac
     1441 tggtcctact ataccggcgt aaatatctcc atcagttctc tcagctatat ccttatatat
     1501 atcgaaatat tgcacctttt tcccccctta ataatttaat acaaacctta acaatatata
     1561 tatacgcggc ttaaattaaa ttattacaga caagaaaaaa taacctgcaa taaatgcagg
     1621 ttaccagttt atccccttaa aaaataccat attttccatt tcctcttttt tatccctgtc
     1681 catcaagtta ggaacagact gtttcacatc tttcccttca taaagaacct tatatagttc
     1741 ctctgtaata ggcatatcta catcgtattt ttttgccaac atataggttg atttggtggt
     1801 tttaatcccc tcaactacca ttcctatttc tctaattgat tcctccacag aggcaccctt
     1861 tcctattaag attcctgcct gtctgtttct gcttaacata ctggtacagg tcactatcag
     1921 atctcctatg ccagctaaac cagaaaaagt gctaggtgat gctcccattt tttcccctaa
     1981 cctggccatt tcaaaaattc ctctcgtcat caaagccgcc ttagtattgt ctccatatcc
     2041 tgacccgtct gatattcctg cggccaaggc aattacattt ttcaatgcac cagccaattc
     2101 tacacctact acatccaggt ttgtataaac tctaaaatct acagtcataa aaacgtcttg
     2161 aatatactgg gctgcatccc tatctgttga tgcggatacc acagttgttg gaatgcctct
     2221 tgcaacctct tctgcatggg aagggcccga aagcattaca tatttattat caggtaaaaa
     2281 ttcttgaaca atctgtgaaa ctctcattaa agtatcattt tctatgcctt tggccacatt
     2341 gacaatgatt tgttccctct ttatatggct accgtattct tttaagatct gccgtatact
     2401 atgggaggga gttgccaaaa gcacaatatc tttatcagaa agaacttctt ctatattgtt
     2461 acttaatttt agttccttag gcagttggac ttccggtaaa tatctgacat tttcgctctt
     2521 ttcttgcatt tcttttaact gcccctgatt tctaacccac atctttacct tatggttttt
     2581 attggccagt aaaataccta gggcagtccc ccaactacct ccacctatga ttcctatttt
     2641 tgctttcatt ttaccaccca cctattttat tctcagttcc ctcagctagc cttttgaaat
     2701 tagatctatg tcttattatg gcaagtagag ctaggagtat agttagactc aaggcccacc
     2761 tgttggaggt tttatccagt aggaaatata aaaaaggctg ggacgataaa aataaaacgg
     2821 agcctaaaga tacatacctg gtcattaaaa ccaccgcaag accaagaact acacctacaa
     2881 gtgcaagtct ccaatctgtc ataagtaaca cacctaagga ggtggccacc ccttttccgc
     2941 ccctaaaccc tataaagatt ggccaattat gtcctaccac agcaaacagg ccggcaatta
     3001 gtccaccatc atatcccata agtgaccttc ctattataac ggaaatcagc ccttttaaaa
     3061 tatcaaaaac aaaggttaag atccccatcc taagacccat caccctaagg gcattagtag
     3121 ccccaacatt cccgctgcca tggcttctga tatctatatc cttgaatatt tttcctataa
     3181 aataggcaga tgaaaaacat cctattaaat aagatatgag aataatctgc aataacttca
     3241 ttattgagct cctttttccc taaattcaaa caatattggt gtacctgcaa atccaaaatg
     3301 accccttatt tgattctcca gatatctttc atatgagaaa tgcattaatt ccttatttct
     3361 aacaaagatt acaaatttag gtggcctaac agatacctgg gttccataat aaattttaac
     3421 cctcctcccc ttatcagagg gtggttgatt cataagtata gcttgattta gaatatcatt
     3481 taaaacacca gtgctaatcc ttagattgta gttattgtca acaatattga ttaattctaa
     3541 taatttttta accctttgac ctgtaagagc agatataaat actaatgggg catagtgtat
     3601 gaagctaagt ttggttctta catctatttc aaattcccta taggtcatgg tttccttttc
     3661 caccagatcc cacttgttta cggctattat tattgccttg ccattttcat gggcatagcc
     3721 ggcaatctta gtgtcctgtt ctgtaactcc ctctgtagca tctattacta agacacataa
     3781 atcagatcta tcaatggccg ttagggttct gactacagaa tatctttcaa ctttttcata
     3841 aatacttctc ttccttctca gaccagcagt atcaataaat acatatttat ttccttcaaa
     3901 ttcatatttg ctatcaatgg catctctggt ggttccaggt atatctgtta ctatggttct
     3961 ttcttctccc aatatatagt ttataagaga tgatttacct acattaggct ttcctataaa
     4021 ggctaccctc atttggtctt catctaaact tgtgtccaca gtttcaggga aaagctttat
     4081 aacttcatca agcaaatcac caatacctaa actttgttcg ctagaaatcc ccataggttc
     4141 accgaaacct agttcataaa attcatataa cctatcccag tgcctatgtg agtctagttt
     4201 attacatact agaataacat tttttccaga tcttctaagc aaattaccta tttccttatc
     4261 tgtactagtc agcccttccc ttccatctac cacaaacaaa ataacatccg cagtatctat
     4321 tgccatttgg gcctgccttt ttatattaga cataatttgg tcttcattat ccggttcaag
     4381 tcctcctgta tctattacag taaaatgatg gttaagccat tctccttcag cataaatcct
     4441 gtctctagtt actcctggag tatcttctgt aattgctatc cgtcttccta ccagcttatt
     4501 aaacaaggtg gatttaccta catttggtct acctactata caaacaactg cctttctcat
     4561 ttgctcacct caatcctcag tatatcaata aaatcttttc ctcttactgg agaaactata
     4621 atatgtcttt ctaacttttt tgccacatct tcaagatcca cactatctaa gaaaatat
//
LOCUS       DATGSI010000152         1468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_578693, whole genome shotgun sequence.
ACCESSION   DATGSI010000152 DATGSI010000000
VERSION     DATGSI010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578693"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..618
                     /locus_tag="VK031_02410"
                     /pseudo
     CDS             439..618
                     /locus_tag="VK031_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            628..1014
                     /locus_tag="VK031_02415"
     CDS             628..1014
                     /locus_tag="VK031_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLS52796.1"
                     /translation="MFSFFDTDKTADTRVVRSEERIRIINGRSNCDAVELGYDLLSPD
                     LKGAIQLCEMTLGPYKYSSKKLDVHDAEEVAVCIKASIELHLEDEIITLNKGDSVRIE
                     KGTPHRWKNPTEKQCAIIFAMTPPLF"
     gene            1283..>1468
                     /locus_tag="VK031_02420"
     CDS             1283..>1468
                     /locus_tag="VK031_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HLS52797.1"
                     /translation="MRKVEKWVNKVPILDKVIKEEPTVWINPGKVKNEDIYEEIDITI
                     KDIIDADRRLRRFAPLLM"
BASE COUNT          531 a          203 c          293 g          441 t
ORIGIN      
        1 ggattgctta aattagagat tgcgagtata tttactggta taatcatata agaattcatg
       61 gaactttagg gtgcatgaca tcaattgaat atagaaatag aggtagcttt catttagtag
      121 cttaggcagt acttagaaag tacattcttg taaaacgcaa gtctctgagc agtttttctg
      181 cctatggcag atagccttga cagccggaac aaatttgtct aaaaaaggtt gtttttctaa
      241 gtattgctga gctttcctct ttgacaaaat aattatttgg aagctagagg aaatcacagc
      301 attattcaga gaatcttttt caaaaggttg tgtcacatat gtttggctac attagaacat
      361 tgattatact tagacatggc atcatttgta ttttatgaac cagtatgcta gaattatata
      421 gaatttttaa tatatggggt gattaatata gatataaaat ctttgggaga caaaattaag
      481 tctgttaggg tcgataaagg gatttcgatc aaggaattag cagaacaaat agacgtatcc
      541 tcatcattat tgagccaaat agagagaggg ctggcaaacc cctcgttaaa taccttgagg
      601 ctaatttcta cacatctaaa tataccaatg tttagctttt ttgatactga taagacggca
      661 gataccagag ttgtaaggag cgaggagcgt ataagaatta taaatgggcg ttcaaattgt
      721 gatgcagtag aattaggata cgacttatta agtcctgatt taaagggagc tatccaacta
      781 tgtgagatga ctttaggtcc ctataagtat agctcaaaaa aattagatgt acatgatgca
      841 gaggaggtcg cagtctgtat aaaagcttcc atagaactac atctggagga tgaaataatt
      901 acgctaaata aaggggatag tgtcaggatt gagaagggaa ctccacatcg ttggaagaat
      961 cctaccgaaa aacaatgtgc tattatattt gcgatgacgc ctccgctctt ctaaagttaa
     1021 ggtaaatcct aaaatataca agtatataag ggatcaaaac cagggcatgt taacatgccc
     1081 tggttttgtt tgttgttatt attagattat tataataaaa ctgaacttaa agcccgatat
     1141 aagaacttct atataggaaa tgtactctac catactgccg cacttctatc acaaaattaa
     1201 atataattaa atttattaaa tagtattgca ttaacttaag taatagttta tactaataaa
     1261 tgtaaagaag gaggtgatgg acttgaggaa agtagaaaaa tgggtaaata aagtcccaat
     1321 attagacaag gtgataaaag aggaaccaac ggtttggatt aaccccggta aagtcaagaa
     1381 tgaagacata tacgaagaaa ttgatataac aataaaggat atcatagatg ctgatagaag
     1441 actaagaaga tttgcacctt tattaatg
//
LOCUS       DATGSI010000153         7796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_673129, whole genome shotgun sequence.
ACCESSION   DATGSI010000153 DATGSI010000000
VERSION     DATGSI010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673129"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VK031_02425"
     CDS             <1..1502
                     /locus_tag="VK031_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HLS52798.1"
                     /translation="VKRNNKEYRQEFKKGDPVSGLEVIGESTDTGTSIHFKPDAQVFD
                     TIEFSFETLEHRFREMAFLNKGIKIVIEDKRKDVKKEFHYEGGIKSFVEYINRNKNPI
                     QKDIMYVEAERDGTLVEVAMQYTDGYSENVFTFANNINTEEGGTHLSGFRTALTRVMN
                     DYGRKYNHIKERDENLTGDDVREGLTAILSVKLPEPQFEGQTKAKLGNSETRGIVETI
                     IYDFLNTYLEENPRVGRTILEKAISSQRARHAARKARELTRKRSIFDNTTLPGKLADC
                     QSNDLDITEIYLVEGDSAGGSAKQGRDREFQAILPLRGKILNVEKARLDRILSSDEIR
                     TLITAFGTGIGEDFDIEKLRYGKIIIMTDADVDGAHIRTLLLTFFYRYMKELLDNGHI
                     YIAQPPLYKVTKGRQEHYLYSDEELEDLLNEIGRTNYSIQRYKGLGEMNPEQLWETTM
                     DPESRILLRVSTEDAVAADEIFTTLMGDKVEPRRRFIQENSRLVTNLDV"
     gene            1513..3936
                     /gene="gyrA"
                     /locus_tag="VK031_02430"
     CDS             1513..3936
                     /gene="gyrA"
                     /locus_tag="VK031_02430"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HLS52799.1"
                     /translation="MDKEIGGKIIDINIEKEMKKSYLDYAMSVIVSRALPDVRDGLKP
                     VHRRILYAMNELGMTPDKAYRKSARVVGDVLGKYHPHGDSSVYDAMVRLAQDFSTRYP
                     LVDGHGNFGSIDGDSPAAMRYTEVRMAPMAIEMLRDINKETIDYGLNFDETLKEPEVL
                     PSRFPNLLVNGSSGIAVGMATNIPPHNLNEIIDGIVMLIDNPDTTIEELMEVIKGPDF
                     PTGAFILGTKGIKSAYKTGRGRLRLRAVAEIEELPRGRRRILVTELPYQVNKANLIER
                     IVDHVREKRIEGISDLRDEADRDGMRIVIELKRDANPNIVLNNLYKYTQMQVTFGTIM
                     LALVDGEPKILNLKEMLNHYIEHQKDVITRRTQFELDKAEARAHIVEGLRIALDHIDE
                     VIKIIRGSKSDADAKENLMGKFGLTDIQAQAILDMRLRRLTGLERDKLDEEYKNLIIE
                     INRLKDIIANERLLFNIIKEEILEIKEKYGDRRRTRIVPASGEIDIEDMIAEEDIVIT
                     LTHYGYIKRMPQSTYRTQRRGGRGIMGLTTRDEDFVEDLFITSTHDTILFFTNKGKVF
                     SLKGYEIPEAGRQARGTAIINILNLTGDERVSAIIPISSYEPGNNLVLATKEGIIKKT
                     KLDSFVNIRRNGLIAITLKDDDELISVKKTDGKQELILVTKKGMSIRFDEEDVRTMGR
                     SAMGVKAISLDEGDQVVAMDLVEEDKYLLVISEQGFGKKTPLAEYRIQNRGGKGLITY
                     NIKEKTGDIVSAKVIDDNDEIMMISFNGTIIRLRSEDISVMGRNTQGVTLMKIKDDKV
                     VAVAKYVED"
     gene            4031..4360
                     /locus_tag="VK031_02435"
     CDS             4031..4360
                     /locus_tag="VK031_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HLS52800.1"
                     /translation="MSLEIRIDFDDRENMWLVKPKGDLDIYTSNKFREDVLSSFEEKE
                     TNILVDGEGLEYVDSTGLGALISILKVIREKDKEMHITNIKPNIRKIFDITELDKLFV
                     IRGEKDE"
     gene            4353..4718
                     /locus_tag="VK031_02440"
     CDS             4353..4718
                     /locus_tag="VK031_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLS52801.1"
                     /translation="MSSENIYLKIPSKADYISLARLTSSAIAHNMNLNIEEIEDIKVC
                     VGEACVNILSLTEVEEISITFNISDDNLAIFVKDVVERIPEGLDDFREGELGLLIIRS
                     LMDEVEFVDGGIKMIKYTK"
     gene            4732..5526
                     /locus_tag="VK031_02445"
     CDS             4732..5526
                     /locus_tag="VK031_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HLS52802.1"
                     /translation="MSNRNHYNKEKEEKMSREEIRDLFRKLKETGDKKIREILIEEHL
                     YIAEILSKRYSNRGIDYDDIYQVASLGLIYSIDRYDIDKGYEFSSFATPTIIGEIKKY
                     FRDKGWTIRVPRRIQELSKKINNSKIHLSQTLQRTPTIKDIAEYLKCTEEEVLEAMEA
                     SKVYTPQSLDISFDSNDDDKNINLADLIGEEDLYYSKVENSDFLRKTIKVLNDVERKI
                     LIERYFNRKTQVSIAKKLDISQMTVSRIEKRVIEKLKKEAEKNMIT"
     gene            5561..6223
                     /locus_tag="VK031_02450"
     CDS             5561..6223
                     /locus_tag="VK031_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HLS52803.1"
                     /translation="MKKRIIEKLTNTALFLEILLTVFLAIGIAIGLVDIVKYFVHISK
                     ASAVDSYGVFKQFLAHILLIVVGVEFMLMLISHSINSMLELILFVIARKMLIYADTML
                     DLVLGTIAIAILFIILKYLPVRSGDDYGDDGGAIYSASTTISRLIEQTGIKLPKDKGR
                     TLGGLTFNLAEESTKPITEGSEFILGNIKLKVLKVTDGLIEKVAIEEISDDKDAKIEV
                     DS"
     gene            6476..7357
                     /locus_tag="VK031_02455"
     CDS             6476..7357
                     /locus_tag="VK031_02455"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006157.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HLS52804.1"
                     /translation="MNQKVKVAILGPGNIGTDLMYKIMRSKNLEMSAMLGIVESEGLK
                     RAGKLGFITSTKGIKYLEENPEVADIVFDATTAKAHSDIHAPILERLGKIAIDLTPAA
                     VGKMVSPMVNMGDALKVKNVNLITCGGQATTPIIYSIGKNVDIEYAEVITSVASKSAG
                     PGTRKNIDEYTRTTARAIEKLTGTARCKVMTIFNPAVPEVAMRNTIYIIPKGDVDIEK
                     IARDVENMVEKIQRYVPGYELTLAPKLDKGLIVTTVTVNGAGDFLPPYAGNLDIETAS
                     AVEIAELYAENLLKGEI"
     gene            7359..>7796
                     /locus_tag="VK031_02460"
     CDS             7359..>7796
                     /locus_tag="VK031_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="catalyzes the formation of pyruvate and
                     acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved
                     in the degradation of phenylpropionate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HLS52805.1"
                     /translation="MKKIRLIDTTLRDGSHAMNHQYTVEQIANICEGLEKAGVYAAEV
                     GHGAGIGGSILNYGFAKHTDKEMLTAAKSKLKNTKLGTLLVPGLGTMEDLKAAKDNGL
                     DMVRIAVHCTEVDVAEQHINLAKKLGLETIAFFMMSHMISAKEL"
BASE COUNT         2927 a         1031 c         1728 g         2110 t
ORIGIN      
        1 aggtaaagcg aaacaataag gaatacaggc aggaatttaa aaagggtgat ccagtttctg
       61 gtttggaagt aataggagaa agcacagata caggaacctc tatccatttt aagccagatg
      121 cccaggtgtt tgatactata gaatttagtt ttgaaacctt ggagcatagg tttagagaaa
      181 tggccttttt aaacaaggga ataaaaattg ttatagaaga caaaagaaag gacgtaaaaa
      241 aggaatttca ttatgaaggc ggtattaaat cctttgtaga gtatattaat agaaataaaa
      301 atcctatcca aaaggatatt atgtatgttg aagctgaaag ggatggaacc ttggtagagg
      361 tggccatgca gtatactgat ggttattctg aaaatgtatt tacctttgcc aataatataa
      421 atactgagga aggtggaacc cacctgtcag gctttagaac tgcattaacc agagttatga
      481 atgactatgg aagaaaatat aaccatatta aggaaaggga cgaaaatcta acaggggatg
      541 atgtaaggga aggattgaca gccattttgt cagtcaaatt accggaacct caatttgagg
      601 ggcaaaccaa ggctaaatta ggtaatagtg aaactagagg gatagtagag accataattt
      661 atgacttttt aaatacctat ttagaagaaa atcctagagt aggtagaacc atcttagaaa
      721 aggcaatcag ttcccaaagg gcaagacatg cagcaagaaa ggctagagaa ttaaccagaa
      781 agagaagtat atttgataac acaacattac ctgggaaatt ggccgattgt caatctaacg
      841 atttagatat taccgaaatc tacctggtgg agggagattc tgctggtggt agtgctaagc
      901 agggcagaga tagagaattt caggccatcc ttcctttgag aggaaaaata ttaaatgtgg
      961 aaaaggctag attagataga attctcagtt cagatgaaat taggactttg attacagcct
     1021 ttggtactgg tataggtgaa gactttgata tagaaaagtt aaggtatgga aaaatcatta
     1081 taatgacaga cgcagacgtg gatggagccc atattaggac cttactctta acatttttct
     1141 ataggtatat gaaggagtta ttggacaatg gtcatattta tatagctcaa cctcctctat
     1201 acaaggtaac taaggggcga caagaacatt acttatatag tgatgaagaa ttagaagact
     1261 tgctaaatga gataggaaga acaaattact ctattcaaag atataagggt ctaggagaaa
     1321 tgaatccaga acaactatgg gaaactacca tggatcctga atcaagaatc ctattaagag
     1381 taagtacaga ggatgcagta gcagccgatg agatttttac aaccttaatg ggagataagg
     1441 tagaacctag aagaagattt atacaggaaa actctaggct agttacaaat ctagatgtat
     1501 aggaggcaat atatggataa agaaattggt ggtaaaataa tagatattaa tatagagaaa
     1561 gagatgaaaa aatcctacct agattatgca atgagtgtta tagtaagcag ggccctacca
     1621 gatgttaggg atgggttaaa acctgtacac aggcggattc tttatgcgat gaatgaatta
     1681 gggatgacac cggataaggc ttataggaaa tctgccaggg tagttgggga tgttttaggt
     1741 aagtatcacc cccacggtga cagctctgtc tatgatgcta tggtaagatt ggcccaagac
     1801 tttagtacca gataccctct ggtagatgga catggtaact ttggatccat agatggagac
     1861 agtccggcgg ccatgcgtta tacagaggtt aggatggccc caatggccat tgagatgctt
     1921 agagacataa ataaggaaac catcgattat ggtcttaact tcgatgaaac cttaaaggag
     1981 cctgaagtat tacctagtag gttccctaat cttttagtca atggttcctc tggtatagca
     2041 gtaggtatgg ctacgaatat tccacctcat aatctaaatg aaattataga tggaatagtt
     2101 atgttgattg ataacccaga tactacaata gaagaattaa tggaagttat aaaggggcca
     2161 gatttcccaa cgggggcctt cattttagga accaagggaa taaaatctgc ctacaagacg
     2221 ggaagaggta ggttaaggtt aagggctgtg gctgagattg aagaattgcc aaggggcagg
     2281 agaagaatct tagttacaga gttaccctat caggtaaata aggctaattt aatagaaaga
     2341 attgtagatc atgtaaggga gaaaaggatt gagggaatat ctgacttaag agatgaggcg
     2401 gatagggatg gaatgaggat tgttatagaa cttaaaaggg atgccaatcc taatatagtc
     2461 cttaataatc tctataaata cacccaaatg caggtaactt ttggtaccat aatgttggcc
     2521 ttagttgatg gggaaccaaa gatattaaac cttaaggaga tgctcaatca ctatatagag
     2581 caccaaaagg atgtaattac aagaagaacc cagtttgaac tagacaaggc agaggccagg
     2641 gcccatatag tggaagggct aaggattgct ctagaccata tagatgaagt tattaaaatt
     2701 attagaggat ccaaaagtga tgctgatgcc aaggaaaacc tgatgggtaa gtttggctta
     2761 acagatattc aggcccaggc tattttagat atgagattaa ggcgcctaac tggcctggaa
     2821 agagataagc tggatgaaga atataaaaac cttattattg aaataaatag gctaaaggat
     2881 attatagcca atgaaagatt actatttaat ataatcaaag aggaaattct agaaattaaa
     2941 gaaaagtacg gtgatagaag aaggacaagg atagttccag catcaggtga gattgatatt
     3001 gaggatatga tagcagaaga agatattgta attactttaa ctcattatgg atatataaaa
     3061 agaatgcccc aaagtaccta cagaacccaa agaaggggag gtaggggaat tatgggatta
     3121 actaccaggg atgaagactt tgtggaggac ttatttatca cttcaaccca tgacacaatc
     3181 ctattcttta ccaataaagg aaaggtattt tccttaaaag gatatgaaat cccggaggca
     3241 ggaaggcagg ctagaggaac tgctattata aatattttaa acctgacagg ggatgaaagg
     3301 gtatcagcaa ttattccgat ttcatcttat gagcctggta ataatctagt tttggctacc
     3361 aaagaaggaa ttattaagaa aacaaaacta gacagctttg taaatatcag gagaaatggg
     3421 ctcattgcta taacccttaa ggatgatgat gagttgatat cagttaaaaa gactgatgga
     3481 aaacaagagt taatcctagt aactaaaaag ggaatgtcta ttagatttga tgaagaagat
     3541 gtaagaacca tgggaaggag tgccatggga gttaaggcaa taagccttga tgaaggagac
     3601 caggtggtgg ccatggactt ggtagaggaa gataaatatc ttttagttat ttctgaacaa
     3661 ggttttggca aaaagactcc tttagctgag tataggatac aaaatagggg aggcaagggg
     3721 ctgattacct ataacatcaa ggaaaaaact ggagatattg tatctgccaa ggtaatagac
     3781 gacaatgatg aaataatgat gatatccttt aatggaacta taatcaggct aagatcagaa
     3841 gatatatcag tcatgggaag aaacacacaa ggagtaactc ttatgaagat aaaagatgat
     3901 aaagtagtag cagtagctaa atatgttgaa gactagtggg aacctaaatt taggttcctt
     3961 cttttaactt tactttatat ccaatatgtt ataaaattgg cttataagac taagtaatag
     4021 gagggttacc atgtctcttg agattaggat tgattttgat gatagggaaa acatgtggct
     4081 tgtaaaacct aaaggcgatc tagatatata cacatctaat aaatttagag aagatgtact
     4141 aagctctttt gaggaaaagg aaactaacat tttagttgat ggagagggct tagaatatgt
     4201 ggatagtaca gggctaggag ccctgatcag tatattgaag gttattagag aaaaggacaa
     4261 ggaaatgcat ataactaata taaagccaaa tataaggaaa atttttgata tcaccgaatt
     4321 ggacaagtta tttgttatta gaggtgaaaa ggatgagtag tgaaaatatt tacctgaaga
     4381 ttcccagtaa ggcagattat ataagcttgg ccagactaac atcatctgcc atagcccata
     4441 atatgaattt aaatattgag gaaatagaag atattaaggt atgtgtcggt gaggcttgtg
     4501 taaatatttt aagtttaaca gaggtagaag agatttctat tacatttaat atatcagatg
     4561 ataatctggc aatatttgta aaagatgtgg tagagagaat tccagaaggg ttagatgatt
     4621 ttagagaagg tgaattaggc ctattgatta ttagatcctt aatggatgaa gttgagtttg
     4681 tagacggtgg tataaaaatg atcaaatata ctaaataagg gttggtagtt aatgtcaaat
     4741 agaaaccact ataataaaga aaaagaagag aagatgagca gggaagaaat tagagactta
     4801 tttagaaaac taaaggaaac tggagataaa aaaataagag aaatactaat agaggagcac
     4861 ttatatatag cagaaatcct gtctaaaaga tactccaata gaggtattga ctacgatgat
     4921 atataccagg ttgcttccct aggtcttatt tattcaatag ataggtatga tattgataag
     4981 ggatatgagt tttctagctt tgcaacacct accataatag gtgagataaa aaaatacttt
     5041 agggacaagg gttggaccat cagggtccct agaaggattc aagagctctc caaaaaaata
     5101 aataattcta aaatccactt aagtcaaaca ctacaaagaa ctcctactat aaaagatata
     5161 gcagagtatt taaaatgtac tgaagaagag gttttagagg caatggaagc tagcaaggta
     5221 tacacacctc agtcgctaga tatttctttt gattcaaatg atgatgataa aaatataaac
     5281 ctggctgact taattggaga agaagaccta tactatagta aggtggaaaa cagtgatttt
     5341 ctaagaaaaa ccattaaggt cttaaatgat gtggagagaa aaatcttaat tgaaagatat
     5401 tttaatagaa agactcaagt gtccattgct aaaaagttag atatttcaca gatgactgtt
     5461 tctcgtatag agaaaagagt aatagagaag ttaaaaaaag aagcagaaaa aaatatgata
     5521 acttaaaata agtggattcc aatagtacgg aggtttaaaa ttgaaaaaga gaattataga
     5581 aaagcttacc aatacagcct tgtttttaga aattttacta acagtctttc ttgccattgg
     5641 gatagccatt ggtttagtgg atatagttaa atattttgta cacatatcta aggctagtgc
     5701 agtggattca tatggagtgt ttaaacaatt cctggcccat atacttttaa tagtagtagg
     5761 ggtagaattt atgcttatgc taatatctca ttccattaat tctatgctgg aattaatcct
     5821 ttttgtaatt gccagaaaga tgctaatata tgctgacacc atgttagatc ttgttttggg
     5881 tacaatagcc attgcaattt tatttataat tttgaaatat ttacctgtta gaagtggaga
     5941 cgactacgga gatgatggag gagcaatata ttctgcatca acaaccataa gtaggcttat
     6001 agagcaaact gggataaaat tacccaaaga taaggggagg accttaggag gtctaacctt
     6061 taacttagca gaggaatcaa caaagccaat tactgaagga agtgagttta tcttaggtaa
     6121 tataaagctt aaggttctta aggtaactga cggccttata gaaaaggtgg ctatagaaga
     6181 aatttccgat gataaggatg cgaaaataga agtagattca taaaaggtgc tggaccaaga
     6241 cttggtccag caccttttta attacatatt aaacctatag tataaataaa aaatatttta
     6301 agctgaatgt ttcaagcaag gcttggcatt cagctttttt agaattttag aggtatatat
     6361 tttgattgta aaaaataatt gaaaaaaatt taacaaaaaa ggattgacat atttataatt
     6421 tataatataa tatcaaccac aggaactaag ttcagtataa tgaaaggaga tcaatatgaa
     6481 tcaaaaagtt aaggtagcaa ttttaggacc aggaaatatt ggaacagatt taatgtacaa
     6541 aatcatgaga agtaagaatc tggaaatgtc tgcaatgtta ggtattgtag aatcagaagg
     6601 tttaaaaaga gcaggcaaac taggatttat tacatctaca aaaggaataa aatacctgga
     6661 ggaaaatcca gaagttgctg atatagtatt tgatgcgaca acagctaagg cacattcaga
     6721 tattcacgca cctattttag agagattagg caagatagcc atagacttaa ctccagccgc
     6781 agttgggaaa atggtttcac ctatggttaa tatgggcgat gccttaaaag taaaaaatgt
     6841 taatttaata acatgtggtg gacaggctac cactccaata atttattcca ttggaaaaaa
     6901 tgtggatata gagtatgcag aggtaataac ctcagtagcc agtaaaagtg ctgggccagg
     6961 tactaggaaa aatatagatg aatatacaag aactactgct agagccattg aaaaactaac
     7021 tggaactgca agatgtaagg ttatgaccat atttaatcca gctgtaccag aagtagcaat
     7081 gagaaataca atctatatta ttccaaaggg agatgttgat atagagaaaa ttgcaagaga
     7141 tgtggaaaat atggttgaaa aaatacaaag atatgtacca ggatatgagc ttacactggc
     7201 accaaaatta gacaaagggc taatagtaac cacagttact gttaacggag caggagattt
     7261 cctaccacct tatgcaggaa atttagatat agaaactgct tcagcagtag agatagcaga
     7321 attatatgcc gaaaatttac tgaaaggaga aatatagaat gaagaaaatt aggttaattg
     7381 ataccacatt aagggatggt agccatgcta tgaatcacca atatactgtg gagcaaatag
     7441 caaatatttg tgaaggctta gaaaaagccg gcgtatatgc ggcagaagta ggtcatgggg
     7501 caggtatagg tggatcaatt ttaaactatg gatttgccaa gcatacagac aaggaaatgt
     7561 tgacagctgc taagtctaaa ttaaagaata caaaattagg gacattatta gttccaggtc
     7621 taggaactat ggaggactta aaggctgcta aggataatgg cctggatatg gtaaggattg
     7681 cagttcattg cactgaggta gatgtggctg aacagcatat aaatctggct aaaaagttag
     7741 gattagaaac catagccttc tttatgatga gtcatatgat ttcggctaaa gaattg
//
LOCUS       DATGSI010000154         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_57697, whole genome shotgun sequence.
ACCESSION   DATGSI010000154 DATGSI010000000
VERSION     DATGSI010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57697"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VK031_02465"
     CDS             <1..304
                     /locus_tag="VK031_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52806.1"
                     /translation="FLGKIVFNTMEGHEITYSLDQFYLDMNNYLFRLRKSRIDPKRLV
                     IKDYILPDVFSFEENQVFFHSLLNHCKVIKKEVRNNQLTSLIQTKTGMYTFKRDLI"
     gene            complement(301..510)
                     /locus_tag="VK031_02470"
     CDS             complement(301..510)
                     /locus_tag="VK031_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52807.1"
                     /translation="MQDFDKISIQEMSKNDMLLIIQALEYTGVNTKIDDFIDLKNSFI
                     KELSYLAEIPESQFIEYLQKETQSL"
     gene            654..2420
                     /locus_tag="VK031_02475"
     CDS             654..2420
                     /locus_tag="VK031_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HLS52808.1"
                     /translation="MKTILTTLNSKYIHSNLAIRYLSKYVEDIKDVALVEFTINQDRD
                     FITAELHKMEADLIGFSTYIWNLNETLEVCQVLKIANPNLKILLGGPEVTFDGEKILK
                     NHPYIDFIIYGEGEESFRDFLSTLDNNRDLSSVKGLIYREGERIIKNQPRPLLADLNQ
                     IPSPYEQVGKEFKNRIVYYESSRGCPFNCQFCLSSTIRGVRYFNIERVKKDLSNLIEG
                     QVRQVKFIDRTFNANKRYAMEIMQFIMDKDPENINFHFEVTAHLIDHELLDFISQAKE
                     GLFQFEIGVQSTKDKTIEAIGRTTDLDKLKTVVRKIKSFGNIHQHLDLIAGLPYESYE
                     RFKKSFNEVYDLKVEKVQLGFLKLLKGSGLRNNQKKYGYKYLNKPPYEIISNNYISYS
                     QIIRLKGIEDLVEKYYNGGYFEHTLEFLIRNFYQGAFDFYEDLLDYWEEKSYHKLSHS
                     RKNLYKHLKNFIKHKGYKDSKILFNLLKYDFLGNNPRTNLPSFLETRSPALSRRTVHQ
                     ILKEEGLLNKYLPEHKNMATKKLINQVQLVSFDINILELIDNGYNPYKKGEETFILLK
                     YMDGQINRYQAWDITELVKELI"
     gene            2420..>2861
                     /locus_tag="VK031_02480"
     CDS             2420..>2861
                     /locus_tag="VK031_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52809.1"
                     /translation="MDYIKDLIYVNKTAISRTYASIKSHWPIIFTGIAYTLLNGVIYY
                     ILSLLLQGPLYLLSGIIMAIITSSFISNYLYLLLGIINYDRLTMQDFKDGFAHFLGKI
                     YGVLFIGYLGSLLLSLVTPILGNKSFVLNMIVSISVFILLNALPE"
BASE COUNT         1060 a          435 c          485 g          881 t
ORIGIN      
        1 ctttttagga aaaatagtct ttaacactat ggaaggacat gagataacat atagcctaga
       61 tcaattctat cttgatatga ataattattt attcagactg agaaaaagca ggatagatcc
      121 taaaagacta gtaattaaag actatatact gccagatgta tttagctttg aggaaaacca
      181 ggtttttttc cattccctat taaaccattg caaggtaata aaaaaagaag ttaggaataa
      241 tcagctaact tctttgattc aaaccaaaac tggtatgtat acatttaaaa gagacctaat
      301 ctagagagat tgggtttctt tttgtaagta ttctataaac tggctttctg gaatctctgc
      361 caggtaagat agttccttaa taaaactgtt ttttaaatcg ataaaatcat caatctttgt
      421 attgactcca gtatattcca atgcttgaat tattagaagc atatcatttt tagacatttc
      481 ctgaatacta attttgtcaa aatcctgcat cctacaaccc ctctccacat attatatatt
      541 ctattctaca tcttttgacc taaacaatca ataggacaaa gtctaagaag ttgaaagaaa
      601 atactacata tggaattaaa atgttataat agaaaaagaa agtaggtgca aagttgaaaa
      661 ccatactaac cacattaaat tcaaaatata tccattcaaa cctagccata agatatttgt
      721 ctaaatatgt agaagatata aaggacgtag cccttgtaga gtttaccata aatcaggaca
      781 gggattttat aacagcagaa ctacataaga tggaggcaga tttaataggt ttttccactt
      841 atatttggaa cttaaacgaa acattagagg tttgccaggt tttaaagata gcaaatccaa
      901 atttaaaaat ccttttaggc gggcctgagg taacctttga cggagaaaag atattaaaaa
      961 accacccata tatagatttc ataatctatg gagaagggga agaaagtttt agggattttc
     1021 tatccacctt ggacaacaat cgggacctat catcagttaa gggtttaatt tatagggaag
     1081 gagaaaggat aattaaaaac caaccaagac ccttgctggc tgacttaaac caaatacctt
     1141 ctccctacga acaggtaggt aaggaattta aaaataggat cgtctactat gaaagctcaa
     1201 ggggctgtcc ctttaattgc caattttgtt tatcttccac cattagaggg gtaagatact
     1261 ttaatataga gagagtcaaa aaagatttgt ccaacctgat tgaaggccag gtaaggcagg
     1321 tgaaatttat agacaggacc tttaatgcca acaaaagata tgccatggag attatgcagt
     1381 ttattatgga taaggatcca gaaaatatca attttcattt tgaagtaact gcccacttaa
     1441 tagaccatga gctcttagat tttatatctc aggccaagga aggtttattt caatttgaaa
     1501 taggggtaca atcaaccaag gataaaacta ttgaggccat aggcagaact acagacttgg
     1561 acaaattaaa gacagtagtt aggaaaataa aatcctttgg aaatatccac caacatttgg
     1621 atctaatagc agggctgcct tatgaatcct atgaaagatt taaaaaatcc ttcaatgaag
     1681 tatatgattt aaaggtagag aaagtacaat taggcttttt aaaactatta aaaggatctg
     1741 gtctaagaaa taatcaaaaa aagtatggct ataaatatct gaataagcca ccctatgaga
     1801 ttatatcaaa taattacatt agctatagcc aaataatcag gctgaagggt atagaggatc
     1861 tggtggaaaa gtattacaat ggaggctact ttgaacatac cttagaattt ttaataagaa
     1921 atttttacca gggagccttt gacttttatg aagatttact agactattgg gaggaaaaat
     1981 cctaccacaa gttatctcat agccggaaaa atctatacaa gcatttaaaa aactttatta
     2041 agcacaaagg atataaggac agcaagattt tatttaacct gttaaaatat gactttttag
     2101 gtaacaatcc tagaacaaat ttaccttcct ttcttgaaac taggtcacct gctttatcaa
     2161 gaaggactgt tcatcaaatc ttaaaggagg agggcctatt aaataagtat ttacctgaac
     2221 acaaaaacat ggccaccaag aaattaatca accaggtaca actggtaagt tttgatataa
     2281 atatattaga actaattgat aatggttata atccttataa gaaaggagaa gagaccttta
     2341 ttttattaaa gtatatggat ggacaaataa ataggtatca agcctgggac ataacagaat
     2401 tagttaagga gctgatatag tggattacat taaagactta atttatgtga ataagactgc
     2461 catcagtagg acctatgcca gtataaaaag ccactggcca ataatattta caggaatagc
     2521 ctataccctc ttaaatgggg taatatatta tattctatcc ctactattac aaggcccatt
     2581 gtatctttta tctggaataa taatggccat tatcaccagt agctttatat ctaattactt
     2641 atacttgcta cttggtataa ttaattatga taggttgacc atgcaggatt tcaaagatgg
     2701 ttttgcccac tttttaggta agatttatgg ggttttattt attggatatt taggaagttt
     2761 attgttatca cttgtaaccc ctattttagg aaataaaagt tttgttttaa acatgatagt
     2821 atctataagt gtatttatac ttttaaatgc cctgccagaa a
//
LOCUS       DATGSI010000155         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_57792, whole genome shotgun sequence.
ACCESSION   DATGSI010000155 DATGSI010000000
VERSION     DATGSI010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57792"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VK031_02485"
     CDS             complement(<1..758)
                     /locus_tag="VK031_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLS52810.1"
                     /translation="MNLLIKDARLIDDKQDLVADLYIEKGKIRKIGKALSYNCKTIDG
                     KDLCLLPAFVDIHAHFRDPGYTYKEDIETGSLSALRGGYTFVNLMGNTDPVCSSMEIV
                     DDVLKRAKNLDLEDIHQSVSITKNFDGESLEHLDLLDERVKVITDDGMGIKSNIVMYK
                     AFKKAKEKGLLILSHAEDEDLTPIDYRVSENLISFRDIYLSQVLETRLHLTHVSTKEV
                     IEAIRRAKGKGNKYLTCDVTPHHISLWDSNEKVNP"
     gene            complement(760..1188)
                     /locus_tag="VK031_02490"
     CDS             complement(760..1188)
                     /locus_tag="VK031_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HLS52811.1"
                     /translation="MLNINSINQGIVIDHIKAGWGLKIFEYLNLDEADFSVALIMNAV
                     SKRHGRKDIIKIANEIDIDLTMLGFIDPNITVNIIENEKITEKINLSLPEKVKGIIKC
                     KNPRCITRDEKNIDHKFVLIDKDNGIYKCDYCDHIYSWEG"
     gene            complement(1182..2111)
                     /gene="pyrB"
                     /locus_tag="VK031_02495"
     CDS             complement(1182..2111)
                     /gene="pyrB"
                     /locus_tag="VK031_02495"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HLS52812.1"
                     /translation="MLKGRHLIDPEDLHLQELEEIFQLTNLIIKNQEDFHNLCKGKIL
                     GTLFYEPSTRTRFSFEAAMLRLGGKVIGFSEPGSSSVMKGESLPDTIRTVAAYTDIIA
                     MRHPKEGAPKYASLYSQVPIINAGDGGHQHPTQTLADLFTILKSKGRLNNLAIGFCGD
                     LKFGRTVHSLIKAITRYQNITLYLISPEELQIPAYIRKEVLEDKNVDFIEVERLEEVL
                     DKLDVLYMTRVQKERFFNEADYIRLRDSYILDLKNLAKASKDLSILHPLPRLKEISTE
                     VDQDPRALYFQQVKNGMYIRMALIMKLLGVDKC"
     misc_binding    2533..2770
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2883..3863
                     /locus_tag="VK031_02500"
     CDS             2883..3863
                     /locus_tag="VK031_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLS52813.1"
                     /translation="MKKGKVVGVSLLLLVLVLALTACTSSPATEDGEEFVIGISQFAE
                     HSALDDARLGFEEGLKELGIDAKIVYQNAQADVATTNTIAQKFVKDKVDLIYAIATPA
                     AQSAKQATHQIPVLFSAVTDPVKSEIVADWEDVGGNVTGTSDMADVNAQLKLFKEIDP
                     KIETIGIIYNTSEPNSEIQIEVVKQLAPGLDLAVETVGVSNINEVPQGLDSLLKKVDA
                     IYILSDNLIASSIELVTNKLIENQVVSVSAEESQVQGGILITHGFSYYELGKQTAQMA
                     KEILVDGKDVSNIPVGLPEKTQVIVNKSTLKALGLDEDLTIFQEAVTVEN"
BASE COUNT         1174 a          743 c          680 g         1334 t
ORIGIN      
        1 ggatttacct tctcattact atcccataaa cttatatgat gaggcgttac atcgcaggta
       61 agatatttat ttcctttacc tttggccctt cttattgcct ctattacctc cttggtactt
      121 acatgggtta ggtgaagtct agtttctagt acctgggata ggtatatatc tctaaaactt
      181 attagatttt ctgaaactcg ataatcaata ggagtaagat cttcatcttc tgcgtgagat
      241 aaaattaaaa gccccttctc cttagctttt ttaaaggcct tatacataac aatgttggac
      301 ttaatcccca taccatcatc tgtaataacc ttaactcttt catctaaaag gtctaaatgt
      361 tccaggcttt ctccatcaaa attcttagtt atagatacag attgatggat atcttccagg
      421 tctaagtttt tagccctttt tagtacatca tctactattt ccattgaaga gcagacagga
      481 tctgtgttcc ccattagatt tacaaaggta tatcctcccc ttaaggccga taggctacct
      541 gtttctatat cctccttgta ggtgtagcct gggtctctaa agtgggcgtg aatatctaca
      601 aaggcaggta gcaagcacaa atcctttccg tctatagtct tgcagttgta ggataaagcc
      661 tttcctatct ttcttatctt tcctttttca atatagagat cagccacaag atcctgttta
      721 tcatcgatta acctggcatc ctttatcaac agattcattt tatccctccc atgaatagat
      781 atgatcacaa tagtcacatt tgtatatgcc attgtccttg tctattaata caaacttatg
      841 gtctatattt ttttcatctc ttgttatgca acgaggattt ttgcatttaa ttatcccctt
      901 aaccttttcc ggcaaggata ggtttatctt ttcagtaatt ttttcatttt cgattatatt
      961 tactgtaatg tttggatcta tgaagcctaa catggttagg tctatgtcta tttcattggc
     1021 aatttttatt atatcttttc ttccatgcct cttacttact gcattcataa tcagggcaac
     1081 tgaaaagtct gcttcatcta ggttcaaata ctcaaaaatc tttaagcccc atcctgcttt
     1141 tatatggtca attactattc cctggttaat gctgtttata tttaacattt atccaccccc
     1201 aaaagtttca taatcagggc cattcttatg tacattccat ttttaacctg ctggaagtaa
     1261 agagcccttg gatcctggtc cacctctgtg ctaatctcct ttagccttgg taaaggatgt
     1321 agaatggata aatccttact ggccttagcc agatttttta gatctaatat ataactatcc
     1381 cttaacctta tataatcagc ctcattaaaa aacctttcct tttgaaccct ggtcatatat
     1441 agaacatcca gcttgtctag aacttcttcc agcctttcaa cttcaataaa gtctacattt
     1501 ttatcttcca aaacttcttt tcgaatatag gctggaatct gcaattcttc tggtgaaatc
     1561 aagtatagtg taatattttg gtatctggta atcgctttga ttagggaatg aactgtacgg
     1621 ccaaatttca aatctccaca aaagcctata gccaaattat ttagtctgcc tttagacttt
     1681 aatatggtga aaagatctgc caaggtttgg gtaggatgtt ggtggccccc atctcctgca
     1741 tttataatag ggacctggct ataaagagaa gcgtattttg gtgccccttc cttaggatgc
     1801 ctcatggcta ttatatctgt ataggctgcc acggttctga tagtatctgg taagctttct
     1861 cccttcatta cagaacttga gcctggctct gagaaaccaa ttactttgcc acctaatctt
     1921 aacatggcgg cttcaaagct aaatctggtc ctagtgctag gctcataaaa tagggtacca
     1981 aggattttcc ccttacatag attatgaaaa tcctcttggt ttttaattat taagttggtt
     2041 aattggaaga tttcctccaa ttcctgaaga tgtaaatctt caggatcaat taagtgtctg
     2101 cctttcagca tggtttttcc ccctttggtt attatacata accttcaagg aatagcgtta
     2161 aaaaaagcct tccaaaaagg aaggcataaa taggcatagc tatacttatg tctcacgctt
     2221 tcctttctaa tctcccggat tagtttaaag gtcttattga tttcattaaa agttatcata
     2281 tgtagtatta aatgtcaatg aaaatttata aaaaaattac tactagatag tggattgctc
     2341 cattaattct accaattact ggcctttata agccataaat ataatcctta taagaaagtt
     2401 atttgagtcc tttctcatac tgacatttta tcagaataat tttaatatga cattatcata
     2461 gagtaaacac acattttcta aaaaagggct tgacattatc acagtaaagc attattatat
     2521 agtttaaata atttaaatgc tatgatggag agaaagatat cactagttat ttacagagag
     2581 ttgggtcagg tgaaagccaa caataacagg atatgtaata ttcactccta agcagatttg
     2641 cttaaaggta acaagtaggc aaatccgaag gcccatcgtt acaagggata ggactgttag
     2701 gtctgattga agtgatggct ttttgccata actagggtgg taccgcggat ataaatcttt
     2761 cgtccctata aatacatcta tgtatgtgtt tatagaggcg aaagattttt ttattaccct
     2821 tttttcctag gaattcaatc aaactaatta tcctaaatat tatattcaag gaggataatg
     2881 gaatgaaaaa aggtaaagtt gttggtgtaa gtcttctttt attagtttta gtattggctt
     2941 tgactgcctg tacctctagt cctgctacag aggatggtga agaatttgtt attggaatat
     3001 cccaatttgc agagcattct gccttagatg atgccagatt aggttttgaa gaaggactta
     3061 aggagttagg catagatgct aaaattgttt accaaaatgc tcaagctgat gtagctacta
     3121 ccaataccat tgcacaaaaa tttgttaaag ataaggttga tctaatttat gccatcgcaa
     3181 ctccagcagc ccaaagtgct aaacaggcta cccaccagat acctgtttta ttcagtgcag
     3241 ttactgatcc agtcaaatct gaaatagtgg ctgattggga agatgtgggt ggcaatgtta
     3301 ctggtacatc tgatatggca gatgtaaatg cccaacttaa actgtttaaa gaaatagatc
     3361 ctaaaattga aaccataggc attatttata atacaagtga gcctaattct gaaattcaaa
     3421 tagaggttgt taaacagctg gcaccaggcc tggacctagc tgtggaaact gttggagtat
     3481 caaatataaa tgaggttcca caggggctgg attccttact aaaaaaggta gatgccatat
     3541 atattctcag tgataaccta atagcttctt ctatagaatt ggttaccaac aagttaattg
     3601 agaatcaggt ggtgtctgtt tctgcagaag aatctcaggt tcaaggtggt attttaataa
     3661 cccacggatt tagctactat gaattaggta agcagacagc ccagatggcc aaagaaattt
     3721 tagtagatgg aaaagatgta tctaacatcc ctgtggggct tccggaaaag acccaggtca
     3781 ttgttaataa gtccactctt aaagccttag ggctagatga ggacctaact atttttcagg
     3841 aggccgtgac agtagagaat taacaagtta ataatttact aaaccagaag gattatatcc
     3901 tccccctagt tctctccttt ttaacagtct a
//
LOCUS       DATGSI010000156         3432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_673328, whole genome shotgun sequence.
ACCESSION   DATGSI010000156 DATGSI010000000
VERSION     DATGSI010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673328"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VK031_02505"
     CDS             complement(<1..1254)
                     /locus_tag="VK031_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52814.1"
                     /translation="MKIHKIAKAGLKGRKKDSFLIILVITLAFIFIVSSTIFQSSIEK
                     TKKEQAFDLYGEWQAAYLKADKEVLDRINKEQDIDLIGKSMIIGKSEGLGLVGTFNQE
                     LLDIGRFSLYKGRFPTKEDEIVLELNQMSLMGLDLEVGQTISVEVTIPKYIDVEENFI
                     KRSQEMINSTEKITNGNYKETRDRLIDLVKELDDIIRLDVNERNYQDIYYNLFENYKR
                     FKVIDHELLGDTRLIFQSILNSFGDLLDDRPKNLEDFLIIRKKLGNQLNRLESFVFYE
                     FRPPINTYKYSEVPFEVVDDVTVVVKNSYIHYYNQGEKVNPEIIRDKGVLTEQSISLK
                     KDFIITGILDTYSDKWDLGDYKAVNAFITEEAGKSFINALYKNTIEDFSNHKFEYNLF
                     LTSTSLQEKLYEGLKDRYPNREEKSV"
     gene            complement(1299..1973)
                     /locus_tag="VK031_02510"
     CDS             complement(1299..1973)
                     /locus_tag="VK031_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS52815.1"
                     /translation="MEIILKAKEIKKTYGQGQLKVEALKPLNLEIEKGFLYAIIGKSG
                     SGKSTLLHILGCLDKPTSGKVYLEGRNVFELKDEELARLRRRRIGFVFQSFNLLEEHT
                     VKENILMPIHLDRGQVDQEYFNRLVKTLAIDDKLNFYPGELSGGQRQRVAIARALISK
                     PAIVLADEPTGNLDEATSKEVMCLIKELAKKFNQTIIIVTHDLEIAEEADKVISLVDG
                     QISSVV"
     gene            complement(2098..2307)
                     /locus_tag="VK031_02515"
     CDS             complement(2098..2307)
                     /locus_tag="VK031_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52816.1"
                     /translation="MLEQLKNKNHKFNNEDENFIDDKKNDFSSRLENEEDKLEQNDII
                     ALIISSILVFGPIFIILGLILKLLI"
     gene            complement(2355..>3432)
                     /locus_tag="VK031_02520"
     CDS             complement(2355..>3432)
                     /locus_tag="VK031_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HLS52817.1"
                     /translation="SKSPEEVSKALLEEHNIARESFKLRRKIEDQNLLIEGKKLSLEE
                     EGLGKEEIEKQLSSQKEKLEEWEKELEKLDEKYQVIGEKIEAMRKKCKNHPDFENYEK
                     HRDKGINLAKLREDEIRVLRKDLQLIFQDPYSSLNPRLTVGQIISEGLHAHNMFAPND
                     ERLQDYILKVMEDCGLAPYFIHRYPHQFSGGQRQRIGIARAVALKPKFIVCDEAVSAL
                     DVSIQSQIINLLLDLKEQEDLTYLFISHDLSVIKYISDRVGVMYLGNIVELAETEELY
                     KRPMHPYTEALLSAIPTTDPDHEREAIILEGDIPSPIDPPTGCKFHTRCKYATDICKK
                     VVPEFEEARPGHFVACHHKLNVDE"
BASE COUNT         1013 a          678 c          408 g         1333 t
ORIGIN      
        1 tacagatttt tcttctctat tggggtatct atcttttagt ccttcatata gtttttcttg
       61 taaagatgta gaagttaaaa atagattata ttcaaacttg tgattggaaa aatcctctat
      121 agtattttta tacagtgcat taataaacga ctttccagct tcttcagtta tgaaagcatt
      181 aactgcttta taatctccca gatcccattt atcactataa gtatctaaaa tccccgtaat
      241 tataaaatcc ttttttaaac ttatagattg ttctgtcaat acgcctttat cccttattat
      301 ttctggattt accttttctc cctgattata ataatgtata taagaatttt ttactacaac
      361 agttacatca tctacaacct caaatggaac ttcactatac ttataagtat taataggtgg
      421 tctaaattca tagaatacaa aagactctaa tctgtttagt tggttaccta gcttttttct
      481 tattattaaa aaatcttcta gattcttagg cctatcatct aataaatcac caaatgaatt
      541 tagaatagac tgaaaaataa gccttgtatc gcctaataat tcatgatcaa taactttaaa
      601 cctcttataa ttttcaaata aattataata gatatcttga taatttcttt catttacatc
      661 caacctaata atatcatcaa gttcttttac caggtcaatt aacctatctc tagtctcctt
      721 gtaattacca tttgtaattt tttctgtact attaatcatt tcttggcttc tctttataaa
      781 attttcttcc acatctatat attttggaat agtaacttct acagatatag tttgtcctac
      841 ctctaaatct aatcccatta ggctcatttg gtttaactct aaaacaattt catcttcttt
      901 agtaggaaat ctacccttat ataggctaaa tctacctata tccaataatt cttggttaaa
      961 tgtccccaca agaccaagcc cctctgattt acctataatc atacttttcc ctattagatc
     1021 tatatcttgt tccttgttta tcctatctaa aacctcttta tctgccttta agtaggctgc
     1081 ctgccactca ccatataaat caaaagcctg ctccttttta gttttttcaa tgctagattg
     1141 gaaaatagtg gatgaaacta taaatataaa ggccaaagtt attaccaaaa taatcaggaa
     1201 ggaatctttt tttcttccct tcagtccagc ctttgcaatt ttatggattt tcactataat
     1261 ccccctttcc taaatttatt ataaaattaa ttcatttttt ataccactga agaaatttgt
     1321 ccatctacta gactaataac cttgtcagcc tcttctgcaa tttctaaatc atgggtaact
     1381 atgattatgg tttggttaaa tttttttgct agctctttaa taagacacat tacttcttta
     1441 cttgtagcct catctaagtt tcctgtaggt tcatctgcta atactatagc tggtttagat
     1501 attaaagctc tagcaatagc caccctttgc ctttgacctc ctgataattc acctggatag
     1561 aaatttagct tatcatctat cgctagagtt tttacaagcc tattaaagta ttcttggtca
     1621 acctgacctc tatctaaatg tataggcatt aatatattct ctttaacagt atgctcctct
     1681 aataggttaa aggattgaaa cacaaaacca attcgtcgtc tacgtaatct tgccaattct
     1741 tcatctttta attcaaaaac attcctacct tccaaataaa cttttccact tgttggttta
     1801 tctaagcatc ctagtatatg taaaagagta gattttcctg agccactttt tcctatgatt
     1861 gcatacaaaa atcctttttc tatttctaag tttagtggct tcagtgcttc aacttttaat
     1921 tgcccctgtc cataggtctt tttaatttct ttagctttta agataatttc cataaaaccc
     1981 ctccaaaaag ttataaaata agcgcattat agtatttatc cataatattc taagctttgt
     2041 aagttatcta tagaaccatt atagaaattt aaatagttta ctacaatgcg cctttcatta
     2101 tattaacaat ttcaaaatta atcctaatat tataaaaatc ggcccaaaaa ccaatatgga
     2161 agagatgatc aaggctatga tatcattttg ttccaatttg tcttcttcat tttctaatct
     2221 tgaagaaaaa tcattttttt tatcatctat aaaattttca tcctcattat taaacttatg
     2281 atttttattt ttcaactgct ccagcattgc ttctattttt tttctagaaa acattattat
     2341 ctaaaccttt ccagttactc atccacattt agcttatgat gacatgcaac aaaatgtcct
     2401 ggtcttgcct cctcaaattc tggtaccacc tttttacaaa tatctgtagc atatttgcat
     2461 ctagtatgga acttacatcc agtaggtgga tctataggac ttggaatgtc tccttccaat
     2521 ataatagctt ctctttcatg gtctggatct gtggtcggta tggctgacaa aagagcctct
     2581 gtataagggt gcataggtct tttgtataat tcttctgttt ctgccaattc tacaatattt
     2641 cctagataca taactcctac cctatcactg atatatttaa ttacacttaa atcatgtgat
     2701 ataaatagat aggttaagtc ttcctgctcc ttaagatcca aaagaagatt aattatttga
     2761 gattgtatag atacgtctag agcagataca gcctcatcac agacaataaa ttttggcttc
     2821 aatgctacag ctctagctat ccctatacgt tgcctttgcc caccagagaa ttggtgtgga
     2881 tatctatgaa taaagtaagg agccaatcca caatcctcca ttaccttcag gatataatcc
     2941 tgtaatcttt catcattagg tgcaaacata ttatgagcat ggagtccctc tgatatgatt
     3001 tggcctacag ttaacctagg gtttaaggat gaataaggat cttggaatat tagctgtaag
     3061 tctttcctta gcactcttat ttcatcttct cttagtttag ctaagtttat tcctttatcc
     3121 ctatgttttt catagttttc aaaatctgga tggtttttac acttcttcct catggcttca
     3181 attttttcgc caataacctg gtatttttca tctagctttt ccagttcttt ttcccattcc
     3241 tctaattttt ccttctgact acttaattgt ttttctatct cttccttgcc taatccctct
     3301 tcttcaaggg ataacttttt accctctatt aaaagatttt gatcttctat ttttcttctc
     3361 agcttaaagg attccctggc aatattgtgt tcttccaata aggctttaga tacttcttct
     3421 ggcgacttgg ac
//
LOCUS       DATGSI010000157         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_390050, whole genome shotgun sequence.
ACCESSION   DATGSI010000157 DATGSI010000000
VERSION     DATGSI010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390050"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VK031_02525"
     CDS             <1..631
                     /locus_tag="VK031_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLS52818.1"
                     /translation="IKEYQNKGIIKEIGITGHSHELLDIAIDTGEFATIQYPYNPVER
                     QGEPVFKKAKENNIGVIVMKPLAGGAIPKGDLCLRFILENENVSVAIPGMDSIEQVLE
                     NARAGIDRRDLTGEEREGLMKEAEALGQEFCRRCGYCTPCSVGIDIPGQFILEGYLTR
                     YNLQDWAINRYNSQDLNASDCTACGDCEPKCPYNLPIINMMQRVAENFG"
     gene            656..1876
                     /gene="dpaL"
                     /locus_tag="VK031_02530"
     CDS             656..1876
                     /gene="dpaL"
                     /locus_tag="VK031_02530"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HLS52819.1"
                     /translation="MIKKSSLIHSHLVDVRRSKEPKVDLDYLSEKNIKKVRNFHEKFN
                     QYSITPLHKLENLAKHIGIKNIFVKDESYRFGLNAFKVLGGAYAIGKYLADKLEVDIS
                     EVDFEYLQSPEVKEKLGEITFVTATDGNHGRGVAWAANQLGQKSVVYMPKGSSQVRLD
                     NIRKEGADASIIDGNYDDAVRLSNEMAEKHGWVVIQDTAWEGYTDIPTWIMQGYGTII
                     DEAMDQLNKEGIEKPSHVFLQAGVGSFAGSIQGYLASKFGDDRPVTIIVEPDDANCLY
                     KSSLSGKREIVSGHMPTIMAGLACGEPNTISYEILRDYADGYFSCPDYVAARGMRIMA
                     APLQGDPQIVSGESGAVGIGVTSLLMEREEYKSLKEDIGLNEDSIVLIISTEGDTDPQ
                     KYKDIVWDGDCRSI"
     gene            2023..3003
                     /locus_tag="VK031_02535"
     CDS             2023..3003
                     /locus_tag="VK031_02535"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HLS52820.1"
                     /translation="MKKVLVLLLVVGLTFSLVACTSDGGEEVDTSDYPSKNIEIIVAY
                     KAGGGTDVGARILASEAEKEIGQPLVIINREGSDGELGYTQLAQAKPDGYTIGFINLP
                     TFVSLTLERDTKYGIDDVEPILNHVYDSGVVVVQSSSELDTIEDFIEYAKKNPGDLTI
                     ANNGTGASNHIGAAHLAHEADIEVTHVPFGGSTDMLAALRGGHVKAAVAKISEVAGLV
                     DSGELKILASFTEERLENFPDVPTLAEKGYKILFGSARALVAPKGTPKEIIDYLHDKF
                     KAAMESTEHMEKAKNADLPIMYMGPEELGEYIKNEEVYLKEIGEILDLTN"
     gene            3070..>3300
                     /locus_tag="VK031_02540"
     CDS             3070..>3300
                     /locus_tag="VK031_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52821.1"
                     /translation="MRKNDFILGFILITICGVFYFMISQLPEEGIIYPIFVTTILLGL
                     TILHLLITYFKTEDVESEEFKNIEKKQLLFVVG"
BASE COUNT         1190 a          470 c          737 g          903 t
ORIGIN      
        1 tataaaagaa tatcaaaata aaggcatcat aaaagaaata gggattacag gtcacagcca
       61 tgaactattg gatattgcca tagatacagg agaatttgcc actatacaat acccttataa
      121 tccggtggaa agacaagggg agccagtatt taaaaaggct aaagaaaata atataggtgt
      181 aatagttatg aagcccctgg ccggtggagc cataccaaag ggagacttat gcttacgttt
      241 tattttggaa aatgaaaatg tatctgtggc cataccaggt atggattcta tagagcaggt
      301 cctggaaaat gctagggcag gaatagatag aagagacctg actggagagg agagggaagg
      361 ccttatgaag gaagcggagg ccttgggaca ggagttttgt agaagatgtg gctattgcac
      421 accttgttca gttggaatag atatacctgg acaatttatt ctagaagggt acctaacccg
      481 atataatcta caggattggg caataaatag atataattct caagacctaa atgccagtga
      541 ttgtacagcc tgtggagatt gtgagccaaa atgtccttac aatctaccta ttataaatat
      601 gatgcagagg gtggcagaaa actttgggta aatccatata aacaaggagg taatgatgat
      661 taaaaaaagt agcttaattc attctcattt agtggatgta agaagaagca aggagcctaa
      721 ggtagacctg gactaccttt cagaaaaaaa tattaagaag gttagaaact tccatgaaaa
      781 atttaaccag tattctataa ctccattaca taaactggag aatttagcta agcatattgg
      841 aattaaaaac atatttgtca aagacgaatc ctataggttt gggttaaatg cttttaaggt
      901 tcttggaggg gcctatgcca ttgggaaata cttggcagat aagttagagg tagatatttc
      961 agaagtggat tttgaatatc ttcaatctcc agaagtaaag gaaaaattag gagaaatcac
     1021 ctttgtaaca gcaaccgacg gtaatcatgg tagaggggta gcttgggcgg ccaatcaatt
     1081 aggacaaaaa tctgtagtat atatgccaaa gggttcatca caggtaaggt tagataacat
     1141 aagaaaggaa ggcgctgatg ccagcattat agatggtaac tatgatgatg cagttagact
     1201 atctaatgaa atggcagaaa aacatggttg ggtagttata caagatacag cctgggaagg
     1261 ttatacagat atacctacct ggataatgca aggatatgga accataatag atgaggccat
     1321 ggaccagtta aacaaggaag gtatagaaaa gccaagccat gtatttttac aagcaggagt
     1381 aggttccttt gctggatcta ttcaaggata tttagcctct aaatttggag atgataggcc
     1441 agttaccata atagtagagc cagatgatgc aaattgctta tataaatcat ctttaagtgg
     1501 gaaaagagaa atagtatcag gacatatgcc aacaattatg gcagggcttg cttgtggaga
     1561 gccaaacact attagctatg aaattttaag agactatgct gatggctatt tctcctgtcc
     1621 agactatgtg gcagctagag gaatgagaat aatggcagcc ccgctacagg gagatccaca
     1681 aatagtatca ggggaatcag gagcagtagg tattggagta accagtctac taatggaaag
     1741 agaggaatac aaaagcttaa aagaagacat aggccttaat gaagactcaa tagttttaat
     1801 cataagtaca gagggggaca cagatccaca aaaatacaaa gacatagtat gggatgggga
     1861 ttgtaggtct atataatata aattttaaaa ccaggtatct aagatacttg gttttatttt
     1921 ttttgcttgc atttacaaaa acatatggtt tatttaacaa taaactaact aaatacttac
     1981 ataaaggtta atactacata aagaaaagaa aggaagagtt taatgaaaaa agtattagta
     2041 ttattattag ttgttggatt aacgttttcc cttgtcgcct gcactagtga tggcggtgaa
     2101 gaggttgata ctagcgacta cccctcaaaa aacatagaaa ttatagtggc atacaaggct
     2161 ggtggaggta cagacgtagg agctaggatt ttagcatctg aagctgaaaa agaaattgga
     2221 caacctctag ttattataaa tagagagggt tcagatggtg agttaggata tactcaatta
     2281 gctcaggcga aaccagatgg atacactata ggatttatta atttaccaac ctttgtaagc
     2341 ttaactttag aaagagacac taaatacggt atagacgatg tagagcccat actcaatcat
     2401 gtttatgatt ccggtgtagt agtagttcaa tcttcatcag aactggatac catagaggac
     2461 tttattgaat atgccaagaa aaatcctgga gacttgacta ttgcaaacaa tggtacagga
     2521 gcatccaacc atataggagc agctcacctg gctcatgagg ctgatataga agtaacacat
     2581 gttccctttg gtgggtcaac agatatgttg gcggctctga gaggtggaca tgttaaagcg
     2641 gcagtagcta agatttcaga agtagctgga ttagttgatt ctggtgagtt gaaaatttta
     2701 gcatcattta ctgaggaaag gctagagaac ttcccagatg ttccaacact ggctgaaaaa
     2761 ggatataaaa tattatttgg ttctgcacga gcactagttg ctccaaaagg tacaccaaaa
     2821 gaaattatag actatttaca cgacaagttt aaggcagcta tggaaagtac tgaacatatg
     2881 gagaaggcaa aaaatgcaga tctgccaatt atgtatatgg gaccagagga gctgggcgaa
     2941 tacattaaga atgaagaagt atacttaaag gaaataggtg aaattttaga tctaactaat
     3001 taaaaggaaa aaaattaatt gggagagaaa atatttcttt cccaattaca tatttaatgg
     3061 gggtgtatca tgagaaaaaa tgatttcata ctaggtttca tactgattac tatttgtgga
     3121 gttttttatt ttatgatttc gcagttacca gaggaaggaa ttatatatcc tatttttgta
     3181 actactatct tattaggatt aactatttta catttattaa ttacctattt taagacagaa
     3241 gatgtagaat cggaggagtt taaaaatata gaaaaaaaac aattgttatt tgtagtagga
//
LOCUS       DATGSI010000158         1105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_390092, whole genome shotgun sequence.
ACCESSION   DATGSI010000158 DATGSI010000000
VERSION     DATGSI010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390092"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..>1105
                     /locus_tag="VK031_02545"
     CDS             119..>1105
                     /locus_tag="VK031_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HLS52822.1"
                     /translation="MGRRNKSTYLIIALTCLILLNLGSMLIVFTKNIDNFKGGEVRLL
                     GKLKKALRVDNIVQEVMKFPFPKKDDKKDPSDNENELEIYDDFIITDALEEYENLIIV
                     KDSSGKDSIENIPEPLNIKKIEVDKDKPYIYIYHTHATETFSPYKDNNYHTTDNKKNM
                     VNIGEILTKVLEAGGHKVIHEKTHHDIPSYNQSYSRSLNSLERVMKKEKNLKIFLDLH
                     RDGIEMDKPNYKKRLATFKTEIEGKPTATFSLVVGPHSPNYEQVLNFAKYIKAVSDVM
                     YPDLCTGIIVKKVGKFNQFVSDYAALLEIGSNGNTLEEVEECIKLVGDVLDVA"
BASE COUNT          416 a          143 c          214 g          332 t
ORIGIN      
        1 tcctggaata gatttaaagg atataaatag atatataagc tagttttaaa acatcatata
       61 ttttacttcc aatgaatata tattttatat tggaagctaa accagggggt aaaataatat
      121 gggaagaaga aataaaagca cttacctaat tatagcactg acatgcctta tcttattaaa
      181 tttagggtcc atgttgattg tgtttactaa aaacatagat aattttaaag gaggagaagt
      241 aaggctatta ggtaaactaa agaaggcctt aagggtggat aatattgttc aggaggtaat
      301 gaagtttccc tttccaaaaa aggatgacaa aaaagatccc tctgacaatg aaaatgagtt
      361 ggaaatatat gatgatttta ttattacaga tgctttggaa gagtatgaga atttaataat
      421 agttaaagat tcatctggaa aagattctat tgaaaatata ccggagcctt tgaacattaa
      481 aaaaatagaa gtagataagg ataaacccta catctatata taccataccc atgcaacgga
      541 aacctttagc ccttataagg ataataatta ccatactact gacaacaaga agaatatggt
      601 aaatattggg gagattttaa ccaaggtttt agaggctggt ggacataagg ttatccatga
      661 gaaaacccac catgatattc cttcttataa tcagtcctat tccagatctt taaactcttt
      721 agagagggta atgaaaaagg aaaagaattt aaaaatattt ctagatcttc atagggatgg
      781 tatagaaatg gataagccaa attataaaaa acgtttagcc accttcaaga cagagataga
      841 ggggaagcct acagctacct tttctttggt ggtagggccc cattccccta attacgaaca
      901 ggtgctaaac tttgctaaat atataaaggc tgtatcagat gttatgtatc ctgatttatg
      961 tacaggtata atagttaaaa aggtgggaaa atttaaccaa tttgtttcag attatgctgc
     1021 tcttttggaa ataggtagta atggaaatac tttagaggaa gtagaagagt gtataaaatt
     1081 ggttggagat gttttagacg tggcc
//
LOCUS       DATGSI010000159         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_154554, whole genome shotgun sequence.
ACCESSION   DATGSI010000159 DATGSI010000000
VERSION     DATGSI010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154554"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VK031_02550"
     CDS             <1..1202
                     /locus_tag="VK031_02550"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HLS52823.1"
                     /translation="TAVFRFFYFNYMGISPLISKEICFNVNIDMDRSIGSLNKEDIDA
                     LYYHFSELVEKIKEGDFTPVYIKDDQGKILAFYSFNLNQFGKANKHFSDSISNILDLV
                     YRKKDINDRLIQRAGSLRRIVQTQLDRALTKYGRQKDELIFSKDRDKFKVYADLISAY
                     LYKMPERAKEVKLENFYDENMATIVVPLDEKLTAVENAQKYYKKYSKLKAAEKQLKRL
                     IYLTRQEVDYLENVLFSIDNSTSLEDLNEIRDELVAEGYIRSSKKTKKKSKTKASKPY
                     HYLSQDNFNIYVGRNNRQNEHLTFRFSHREDLWLHVQNMPGSHVIIKRNNQEIPDSTL
                     EEAALLAAYFSKGRQDSKVAVDYTERKNVKKMPGGKLGMVIYENFKTIFVSPTKEAVK
                     KIKKVET"
     gene            complement(1205..1741)
                     /gene="pyrR"
                     /locus_tag="VK031_02555"
     CDS             complement(1205..1741)
                     /gene="pyrR"
                     /locus_tag="VK031_02555"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HLS52824.1"
                     /translation="MKKKVMIMDDKAIFRATTRIANEIIERNKGIQDLVLVGVKTRGV
                     PFAQRLASKIQEIEGKPVKVYDLDITLYRDDLSEIDYFPVVNEKFEGDITDKIVVLVD
                     DVIYTGRTVRAALDALVEKGRPQKVQLAVLIDRGHRELPIRPDYVGKNVPTSKKEIVD
                     VKFKEVDKKDEVLIKDKR"
     gene            complement(1750..2655)
                     /locus_tag="VK031_02560"
     CDS             complement(1750..2655)
                     /locus_tag="VK031_02560"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLS52825.1"
                     /translation="MELIDFTVEKDENVRLDYYLAQRIDRVSRTYIQNLIKKGLVLVN
                     QKAEKPRYLIKKGDHISISLPKPKEIEVLPEDIPIDIVYQDDDIAIINKKQDMVVHPA
                     PGNYEGTLVNALLFNLDSLSSVNDPIRPGIVHRLDKDTSGLLIVAKNNHAHNFLVNEL
                     KERKIIRQYLALVHGVIKKDKGIIDLPIGRDPKNRKRMAVVYENSKNALTKFKVLKRF
                     ANYSLVEAQLETGRTHQLRVHFAHIGHPIVADPVYSRRKNRFNLKGQLLHAKKLCFIH
                     PSTGEYLEFETEMPNRFKKILKKIK"
     gene            complement(2664..3119)
                     /gene="lspA"
                     /locus_tag="VK031_02565"
     CDS             complement(2664..3119)
                     /gene="lspA"
                     /locus_tag="VK031_02565"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HLS52826.1"
                     /translation="MVLILSLAIIILDQVSKHLAIKYLKNKKPYVIVENFFQLSYVEN
                     RGAAFGILQHKRSLFIIFTIIIILILSLYLFRNYNLLNKFTKLAFALLIGGAIGNLID
                     RIRFGYVVDFISFRLFNRYDFPVFNVADISIVASTFIIIYLVLFDQIEI"
BASE COUNT         1103 a          597 c          508 g         1172 t
ORIGIN      
        1 agacggcagt ttttcgtttc ttttacttta actatatggg tattagccca ttaattagca
       61 aggagatctg cttcaatgtt aatattgata tggatcgaag tataggaagt ttaaataaag
      121 aagatataga cgccttatac tatcattttt ctgagctggt tgaaaaaatt aaagaaggag
      181 atttcacccc tgtatatata aaagatgacc aaggaaaaat cctggctttt tattctttta
      241 atctaaatca atttggtaaa gctaataagc atttttcaga ttcgatttct aatattttgg
      301 atcttgtgta taggaaaaag gatattaatg ataggcttat acaaagggcc ggctcattaa
      361 gaaggattgt tcagacccaa ttggaccggg ccttaaccaa gtatggaaga cagaaagatg
      421 agttaatctt cagtaaggat agagacaagt ttaaagtcta cgccgatctt atatctgcct
      481 acttgtataa gatgcctgaa agggccaaag aagtgaaatt ggaaaacttt tatgatgaaa
      541 atatggccac tatagttgtt cctttagatg aaaaacttac tgctgtggaa aatgctcaaa
      601 aatactacaa aaaatattca aaactaaagg ctgctgaaaa gcaattaaaa agattgatct
      661 atctaacccg gcaggaagtg gattatttag aaaatgtatt gtttagtata gataattcca
      721 cttctcttga agatttgaat gaaatcagag atgagttagt tgctgaaggc tacattaggt
      781 cttcaaaaaa aacaaagaag aaaagtaaaa ctaaggcctc taagccctat cattaccttt
      841 cccaggataa ttttaatatt tatgtaggga gaaacaatag gcagaatgag caccttactt
      901 ttagattttc tcaccgggaa gacttgtggc ttcatgtgca aaatatgcca ggtagccatg
      961 taataattaa aagaaataat caagaaatcc ctgattccac tttggaagaa gctgcccttt
     1021 tagcagccta ctttagcaaa ggcagacaag attccaaggt ggctgttgac tatactgaaa
     1081 ggaagaatgt caagaaaatg cctggtggaa aattaggtat ggtaatatac gaaaatttta
     1141 aaacaatttt tgtatcacct accaaagaag ctgtcaaaaa gattaaaaag gtagaaactt
     1201 aattctacct tttatcttta atcagtacct catctttttt atctacctct ttaaacttta
     1261 catctacaat ttctttcttg gatgtaggta catttttccc cacatagtca ggcctaatag
     1321 gcagttctct atgacctcta tctattagta ctgccaactg aaccttttgg ggcctgccct
     1381 tttcaactaa agcatctaaa gcggctctga cagtccttcc tgtatagatt acatcatcta
     1441 ctaggacaac aattttatca gtaatatctc cttcaaattt ttcattaact actggaaaat
     1501 aatctatctc tgataaatca tccctataaa gcgttatgtc taagtcatat acttttacag
     1561 gtttaccttc tatttcctga atcttgctgg ccaacctttg ggcaaagggc actcccctgg
     1621 ttttaactcc tactaaaaca agatcttgaa tccccttgtt tctttcaata atttcattgg
     1681 caatcctagt ggtagctcta aaaatagcct tatcatccat aatcataacc tttttcttca
     1741 taaaatcacc tactttattt tctttaaaat tttcttaaat ctattgggca tttcagtttc
     1801 aaattctaaa tattccccag tactagggtg gataaagcat aatttttttg catgtaatag
     1861 ttgtcccttt aaattaaacc tgttttttct tctagaataa accggatctg ccacaatagg
     1921 atgtccaata tgggcaaaat gaacccgcaa ttgatgggtc ctgccagttt ccaattgggc
     1981 ttcaaccagg ctgtaattgg caaatctttt tagaacctta aatttagtaa gtgcattttt
     2041 gctattttca tatactactg ccattctttt tctattttta ggatccctgc caataggtaa
     2101 gtcaattatg cccttatctt tttttattac tccatgtact agagcaagat attgccttat
     2161 aatttttctt tcttttagtt catttactag aaaattatgg gcatgattat ttttcgctac
     2221 tattagtaaa ccagatgtat ccttgtctaa tctatgtact attcctggtc taataggatc
     2281 atttaccgaa gacaggctgt ctaagttaaa taataatgca ttaactaaag tcccctcata
     2341 gttcccaggc gctggatgga ctaccatgtc ttgttttttg tttataattg caatatcatc
     2401 atcctgatat actatatcta taggaatatc ttcaggtaaa acttcaattt ctttaggctt
     2461 aggcaggcta atagaaatat ggtctccctt tttaattaag tacctaggtt tttcagcctt
     2521 ttggttgact aaaaccaggc ctttttttat taaattttgg atatacgttc tggaaaccct
     2581 gtctattctt tgggccagat aatagtctaa tcttacattt tcatcctttt ctactgtaaa
     2641 atctattagt tccattaaat cacctatatt tcaatctgat caaatagcac caaatatatt
     2701 atgataaatg tgctagctac aatactaata tctgccacat taaatacagg aaagtcatat
     2761 ctattaaata gtctaaaact aatgaaatct acaacatagc caaatctaat tctatcaatt
     2821 aaatttccta tggccccacc tattaagagg gcaaaggcta gtttggtaaa tttattaagt
     2881 aagttataat tcctaaacaa atacagtgac aagattaaaa taataattat agtaaaaata
     2941 ataaataaac tacgtttgtg ttgtaatatt ccaaatgcag cgcccctatt ttccacatag
     3001 cttagctgaa aaaagttttc aactattaca taaggtttct tgttttttaa atatttaata
     3061 gccagatgtt tgctgacttg atctagtatg attattgcaa gggatagaat taataccatc
     3121 ttcttcctcc atttatataa atttatattc tattttatag taataaactg ttaatatcaa
     3181 taattaaata aacttctccc ataaccttgg agaagtttat ttaagtgtat cattataata
     3241 ctcctgggat atattctcaa tcttttgact ctgcccctta tttgtttcat ccataatccc
     3301 catattgtca cctgtactat aggaaggatc atttggaaca atgttaaatt tagccacatc
     3361 ttgataagca tcttccctat
//
LOCUS       DATGSI010000160         1417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_11681, whole genome shotgun sequence.
ACCESSION   DATGSI010000160 DATGSI010000000
VERSION     DATGSI010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11681"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VK031_02570"
     CDS             complement(<1..872)
                     /locus_tag="VK031_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLS52827.1"
                     /translation="MNMEQLTINTIRILSADAIERANSGHPGLPLGAAPMAYTLWAKN
                     MNHNPKKPDWINRDRFILSAGHGSAMLYSLLHLFDYGVSIDDLKKFRQLGSNTPGHPE
                     YGYTVGVETTTGPLGQGISTAVGMAMAEAHLSSKFNRDDYNIVDHYTYVIAGDGDMME
                     GITYEATSLAGTLGLGKLIVLYDSNSITIEGGTDLAFREDVTKRFEALGWETLEVEDG
                     NDIEAIYEKIEQAKKNLSQPSLIKITTEIGYGSPTKQGKSSVHGAPLGVEELKGMKEY
                     FNWKEEDFVVPDQV"
     gene            complement(901..>1417)
                     /locus_tag="VK031_02575"
     CDS             complement(901..>1417)
                     /locus_tag="VK031_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLS52828.1"
                     /translation="HFVHDNQIYGLTKGQGSPTTAKGQVTTLQFDGVKTEPLKPLALS
                     LTMGAGFVARGFSGDIPHLVELMKEAILYKGYAHLDIFQPCVVWNKVNTFGWYKQRVY
                     QLPEDYDYTNYEEAYKKALEFGDKIPIGILYKVEKPTYEDKFEFVKNGPPLVDVELNP
                     LDAEKLMEEFR"
BASE COUNT          377 a          308 c          252 g          480 t
ORIGIN      
        1 tttacttggt caggaacaac gaagtcttct tccttccagt taaagtattc tttcatacct
       61 tttaactctt ctactcccaa gggagcaccg tgcactgagc ttttaccctg ctttgtagga
      121 ctgccatagc ctatttctgt agtgatttta attaggcttg gctgggataa gtttttctta
      181 gcctgttcaa ttttttcata aatagcttca atatcattgc catcttcaac ttctagggtt
      241 tcccatccta aagcttcaaa tcttttagtt acatcttctc tgaaggctaa atctgtccca
      301 ccttctatgg tgatggagtt agaatcatac aggacaatta attttcctaa gcctaaggtt
      361 ccagccaggg aggtagcctc ataagtaatc ccctccatca tatctccatc tccagctatt
      421 acataggtgt agtgatcaac tatattatag tcatccctgt tgaatttgct ggacaaatgg
      481 gcctctgcca ttgccatgcc aacggccgta gatatacctt gacctaaggg tccagtagtt
      541 gtctctactc caacagtgta gccatattca ggatggcctg gagtgttgct tcctagctgt
      601 ctaaattttt tcaagtcatc aatgcttact ccatagtcaa aaaggtgaag tagtgagtaa
      661 agcatggctg agccatggcc tgcagacagg ataaatctgt ccctatttat ccaatctggc
      721 tttttggggt tatggttcat attttttgcc cataatgtat aagccatagg tgcagcacct
      781 aagggtaaac cagggtgtcc tgagttggcc ctttctatgg catcagctga caaaattcta
      841 atggtattaa tagttaattg ttccatattc atatagcctc tccttacctt tatgttaatt
      901 ttatctaaat tcctccatca gcttttcagc atctaaagga tttagttcaa catcaactaa
      961 aggtggacca ttttttacaa attcaaattt atcttcatag gtaggctttt caaccttata
     1021 taggatgcca attggaattt tatctccaaa ttctaaggcc tttttataag cttcttcata
     1081 gtttgtatag tcatagtctt caggtagctg ataaaccctt tgcttatacc agccaaaggt
     1141 gttcacctta ttccaaacta cacaaggctg gaaaatatct aagtgggcat aacccttata
     1201 taagattgct tccttcatta gctctactaa atggggtata tctcctgaaa atcctctggc
     1261 cacaaagcca gcccccatgg ttagagatag ggctaaaggt ttaagcggtt ctgtctttac
     1321 accatcaaat tgtaaggtag ttacctgccc cttagctgtt gtaggtgagc cttgaccttt
     1381 tgttagacca taaatttgat tatcatgtac aaaatgt
//
LOCUS       DATGSI010000161         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_390319, whole genome shotgun sequence.
ACCESSION   DATGSI010000161 DATGSI010000000
VERSION     DATGSI010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390319"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VK031_02580"
     CDS             complement(<1..646)
                     /locus_tag="VK031_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLS52829.1"
                     /translation="MGETIIYEDYIKELLELTKDPTKEDVSRVLNKAKDNIGLSYKDI
                     ALLLQVDDKEVMKEVYKTAGEIKRKIYGNRIVIFAPLYVSNFCINNCLYCGYKKENQI
                     PRRRLTIEEIQKEVTQLEKMGHKRLALEAGEDPSNCHIDYIVDAIKAIYDTYDENGNI
                     RRVNVNIAATTVDNYKKLKDAGIGTYILFQETYHRATYEAMHLSGPKKDYDYHLT"
     gene            complement(631..1698)
                     /gene="hydE"
                     /locus_tag="VK031_02585"
     CDS             complement(631..1698)
                     /gene="hydE"
                     /locus_tag="VK031_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HLS52830.1"
                     /translation="MVNEKYLFKYLINKLHKENFLNEEELIFILDNVNIYGRDYLMEK
                     ADETRKKFYGNRVYLRGLVEISNYCKRKCKYCGINALNKNIHRYRLSKDRILKSCEDG
                     YNLGFRTFVLQGGEDNYFTDELLVGIIMELKERFPDAAVTLSLGERDHESYERLYLAG
                     ADRYLLRHETINPKLFEAIHINSHYDERIESLWNLKRIGYQVGAGFMVGIPNQTTEDL
                     ARELLFLKELEPEMVGIGPFTPHRDTVYKDELAGTLEDTITILALIRLFLPKALIPAT
                     TALASIHRDGRELGLKAGANVVMPNLSPVDVRKDYSLYDGKVSTGYEAAEYRKQLEEE
                     IRRIGYIVDMSRGDYIRWEKQ"
     gene            complement(1800..>2703)
                     /locus_tag="VK031_02590"
     CDS             complement(1800..>2703)
                     /locus_tag="VK031_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HLS52831.1"
                     /translation="TAFAADMTVIEESQEFIERLKSNEDLPLFTSCCPAWVKFAENRY
                     PELRDNISSCKSPQQMFGAVIKEYYKKKDQEDGKETIMISVMPCTAKKAEAAREEFEN
                     DGIRDVDLVITTQELARLIVEMGIVFHELEDESLDMPFGLASGAGIIFGATGGVTEAV
                     VMRLLKDKPDHSEKEVIFKEVRGLNNIKEASFTVDDREVKMAIVHGLKNADELIKKIQ
                     SGEKAYDFIEVMACPGGCVSGGGQPAPVDIKMRAQRASGLYKIDRKSFIKSSEHNAFV
                     TDLFEELLKDKKHLIHVHKKANTI"
BASE COUNT          724 a          606 c          388 g          985 t
ORIGIN      
        1 cagttaaatg ataatcatag tccttcttag gtccacttaa gtgcatagcc tcataggtgg
       61 ccctatgata ggtctcttgg aataatatat aggtgcctat acctgcatct ttaagctttt
      121 tataattatc taccgttgta gcagctatat taacattaac tctacgtata ttgccatttt
      181 catcatatgt atcatatatg gcctttatgg catctactat atagtctata tggcagttgc
      241 taggatcttc accggcctcc agtgccaaac gcttatggcc catcttttcc aactgggtga
      301 cctctttttg aatctcctct atagtaagcc ttcttctagg tatttggttt tcttttttat
      361 agccacagta tagacaattg tttatgcaaa aattgcttac ataaagcgga gcaaatatga
      421 ctattcgatt tccatagatc tttctcttaa tttcacctgc tgttttataa acttccttca
      481 taacttcctt atcatctact tgcaataata gggcaatgtc tttatagctt aagcctatat
      541 tgtccttagc tttatttaag accctactta catcctcttt agtaggatcc ttggtaagct
      601 caagtaattc ctttatatag tcctcataaa ttattgtttc tcccatctta tataatcacc
      661 tcttgacata tctactatat atcctattct tctgatttct tcctccaact gctttctata
      721 ttctgctgct tcatatcctg tactgacctt tccatcatat agggaatagt cctttctcac
      781 atctactggg gatagattgg gcattaccac attggctcct gcttttaaac ctaattccct
      841 tccatcccta tgtatacttg ctaaggctgt tgtggctggt attagggcct taggcaggaa
      901 taatctaatc aaggctaata tggtaatggt atcctctagg gttccagcta gttcatcttt
      961 atagaccgta tccctatggg gtgtaaatgg gcctatgcct accatctccg gttccaattc
     1021 tttaagaaag agtaattccc tagctaaatc ctctgtagtc tgattaggga ttccaaccat
     1081 aaatcctgca cctacttgat agcctatcct ctttaaattc catagggact ctatcctttc
     1141 atcatagtgg gaatttatat gtatagcttc aaacagcttt ggatttatag tttcatgacg
     1201 gagtaaatac ctatctgctc cagctaaata caacctttca taagattcat ggtccctttc
     1261 tcccagggat agggttactg ctgcatctgg aaatctttcc ttaagctcca ttatgatccc
     1321 tacaagtagt tcatcggtaa aataattatc ttctccaccc tgtagtacga aagttctaaa
     1381 accaagatta tatccatctt cacaggattt taatattcgg tccttactta acctgtatct
     1441 atggatattt ttatttaacg catttattcc acagtattta cactttcgtt tacaatagtt
     1501 gctaatctct actaagcccc ttaaatagac cctattgccg taaaatttct tcctagtttc
     1561 atcggccttt tccattaaat aatcccttcc atagatattc acattatcta ggatgaagat
     1621 caattcctct tcatttaaaa agttttcctt atgtaatttg ttgattaaat atttaaataa
     1681 atatttttca ttgaccataa tatacctcac attttattaa tactatggct aatccaaaat
     1741 ctttcctaga aaaggaggat ccttttctag gaatagatta aggagaatat attgaagatt
     1801 tatatagtgt tagctttttt atgaacatga ataagatgtt ttttatcctt caatagctct
     1861 tcaaataggt ctgtaacaaa tgcattatgt tctgagctct taataaagga ttttctatcg
     1921 atcttatata gcccactggc cctttgtgcc ctcattttaa tatctactgg agccggttga
     1981 cctccaccgg atacacaacc acctggacat gccataactt ctatgaaatc ataagccttc
     2041 tctccggatt ggatcttttt aatcagttca tcggcatttt taagcccatg gactatggcc
     2101 atctttactt ctctatcatc tacagtaaat gaagcttcct taatattatt taatcctctg
     2161 acttctttaa atatgacctc tttttcacta tgatctggct tatcttttaa caatctcatt
     2221 actaccgctt cagtaactcc tccagtagca ccgaatatta ttccagcacc actggccaat
     2281 ccaaagggca tatctaggga ttcatcttcc aactcatgga atactattcc catttccaca
     2341 attaaccttg ccaactcctg ggtagttatg actaggtcca catcccttat gccatcattt
     2401 tcaaactctt ccctggctgc ttctgccttc ttagccgtac atggcattac ggatatcatt
     2461 atggtctctt ttccatcttc ctgatccttc ttcttataat attccttgat caccgctcca
     2521 aacatctgtt gaggtgattt acaggaagat atattatccc ttaattcagg gtatctattt
     2581 tctgcaaact ttacccatgc agggcagcag gatgtaaata gtggtagatc ctcattggat
     2641 ttaagccttt ctataaattc ttggctttct tcaatgacgg tcatatctgc agcaaaggcc
     2701 gta
//
LOCUS       DATGSI010000162         1286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_390439, whole genome shotgun sequence.
ACCESSION   DATGSI010000162 DATGSI010000000
VERSION     DATGSI010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390439"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1286
                     /locus_tag="VK031_02595"
     CDS             <1..>1286
                     /locus_tag="VK031_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52832.1"
                     /translation="EDGKVTQYKTDVEDGYGDYSRDNLLNTFSFKGGVTEEEWFNRVF
                     IPHNDIRFSTVIKGIKDKDSSDIIYYRQRFNSGGQQYSVSNLDDNNLRAFNPFNEVNT
                     SSNVSHNLISVRSKNVNGKTVKYIDFEEDIRSRLKIEINVTSVSGIYTASDMFYVANL
                     KVNPEASGTVNEYDISDIVGEVKELEKLNGGRTETFTIDVENDTVFSGGLIIGAKHAL
                     DGVPATDIRYIINSLTVTVYGSEDDPSTVGIKYNEEFNVFDSIPKEISIRDFLKSVML
                     TSNLFLTVDKEDSTKISLYTYDEFYDKVLNFDRKSDVDWSKYNISSNISLPRGYDFLY
                     KEDKDIMNTTYTRLWNEVYGTKRKIDSSGLTEVKKVELMFSPTIPLRSVGDFELPVIY
                     EAEGIGIGEKKQMKSNLRLLMFSGVVNTSTFVPDTT"
BASE COUNT          455 a          151 c          261 g          419 t
ORIGIN      
        1 agaagatggt aaagttactc aatataagac agatgttgaa gatggttatg gtgactattc
       61 cagagataac ttattaaaca ccttctcttt caaaggaggg gttacagagg aagaatggtt
      121 caatagagtt tttattcctc acaatgacat taggttttct acagtaatta aggggattaa
      181 agataaagat agttctgata ttatttatta tagacagagg tttaatagtg gaggtcagca
      241 gtattcagtt tctaatttag atgataataa cttacgtgcg tttaatcctt ttaatgaagt
      301 aaatacttct tctaatgttt ctcataatct tatttcagtg aggagtaaga atgttaacgg
      361 taagacagtt aaatacatag actttgaaga ggatataaga agtaggttga aaatagagat
      421 taatgtaact tctgtatcgg gaatttacac tgcttcggat atgttctatg tagctaattt
      481 aaaagttaat ccagaagctt ctggaactgt aaatgaatat gatatttcag acatagttgg
      541 agaggtcaaa gaattagaaa aattaaacgg aggaagaact gaaacattta caatagatgt
      601 tgaaaatgac actgttttta gtggagggct tatcataggt gctaaacacg ctttagatgg
      661 agttcctgct actgatataa gatatattat aaacagcctt actgtaacgg tctatggtag
      721 tgaggatgac ccttctactg taggtattaa atataatgag gagtttaacg tattcgattc
      781 tattcccaaa gagatttcta taagagattt tcttaaaagt gttatgctaa cttctaactt
      841 atttctaaca gtagataagg aagatagtac taaaatttcc ttatacactt atgatgagtt
      901 ttatgataag gtgttaaact ttgatagaaa gtcggatgta gattggagta agtataacat
      961 ctcttctaat atatctcttc ctagaggtta tgatttctta tataaagaag ataaggatat
     1021 tatgaacact acttatacga gactatggaa tgaggtttac ggaactaaga gaaaaataga
     1081 tagtagcgga ttaacagaag ttaaaaaagt ggaacttatg tttagtccta ctattccttt
     1141 aaggtctgta ggagactttg aattaccagt tatatacgaa gcagaaggta taggaatagg
     1201 ggaaaagaag cagatgaaat ctaatcttag gttacttatg ttcagtggag ttgtaaatac
     1261 gagtacattt gtgccagata ctacca
//
LOCUS       DATGSI010000163         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_390500, whole genome shotgun sequence.
ACCESSION   DATGSI010000163 DATGSI010000000
VERSION     DATGSI010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390500"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VK031_02600"
     CDS             <1..1398
                     /locus_tag="VK031_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HLS52833.1"
                     /translation="HKTEQGGEFVVNRLRSGYHSHKLKPYRQLARAEKKTVKAEVNYL
                     YKKSLGDNPKPTTNPISRWQQKQAIKKEYIAKRYGKSAKTTKETVTNTKKTVGKLADT
                     TEKITKFAVKNHKAILIILGIGAALLFLMSAISSCSVILEGGLQSVVATSYTSEDEDI
                     IAVDEDYTSLEQALQVRIDNIESEYPGYDEYQYHLDEIGHNPFTLASYLTAKLQVYTR
                     SEVESELKALFDEQYTLTTHEEIQVRYRTETRTDTWTDSDGNTHSDTYTVEVPYDYYI
                     LHVTLKNKDISLIASNNLTIDQYEMFGVYMETQGNKPYLFAGNIYAPNKGEYTDYDIP
                     PDALTDPDFEALIKEAEKYLGYPYVWGGSSPSTSFDCSGFVCWVLRESGVYNIGRTTA
                     QGIFNECAKIPPSESRPGDIIFFTGTYASSGAVSHVGIYVGNGMMIHCGNPIQYASVN
                     SPYWTEHFYAYGRLN"
BASE COUNT          578 a          262 c          320 g          426 t
ORIGIN      
        1 cataaaactg aacaaggtgg agaatttgtt gttaataggc taagaagcgg ttatcacagc
       61 cataagctaa aaccttatag acagcttgca agggcagaaa aaaagacagt taaggctgag
      121 gtaaattacc tttataaaaa gagtttagga gacaatccaa agcctactac taatccaatt
      181 tcccgttggc aacaaaaaca ggcaattaaa aaggaataca ttgcaaaaag gtatgggaaa
      241 agtgcaaaaa caactaaaga aacagttaca aacactaaaa aaacagtggg aaagctagca
      301 gatacaacag agaaaataac aaagtttgct gttaaaaacc ataaggccat cttgattatt
      361 cttggaattg gagcagcgct tttattttta atgagtgcta tttcatcttg ttctgttatt
      421 ttggaaggtg gacttcaaag tgtagtagct acttcctata catcagaaga tgaggatatt
      481 attgctgtag atgaagatta cacctcattg gaacaggcac tacaggtacg gattgataat
      541 atagaaagtg aatatccagg ctatgatgaa taccaatatc atttagatga aatagggcat
      601 aatcctttta cccttgcctc atatctaacg gcaaagctac aggtttatac tagatctgaa
      661 gtagagtcag agttaaaagc cttgtttgat gagcaatata ccttgactac ccatgaggaa
      721 atacaggtgc gttatcgaac ggaaacaagg actgatacat ggacagatag tgatggcaat
      781 acccatagtg atacctacac agtagaagtc ccttacgatt attatattct tcatgtaacc
      841 ttgaaaaaca aggatattag tcttattgcc tcaaataatt taaccataga ccaatatgaa
      901 atgtttgggg tctatatgga aacacagggc aataagccct acctatttgc aggaaatatc
      961 tatgctccta ataagggtga atatacagac tatgatatac caccagatgc actaactgac
     1021 cctgactttg aagcccttat aaaagaggca gaaaaatatt tgggataccc ctatgtatgg
     1081 ggcggaagtt caccctctac tagttttgat tgctcaggct ttgtatgttg ggtactaagg
     1141 gaatcaggag tttataatat tggaagaaca acagcacaag gtattttcaa tgagtgtgca
     1201 aaaattccac caagcgaatc aagaccggga gatattatct tctttacagg cacttatgca
     1261 agtagtggtg cagtatccca tgttggtata tatgtaggaa acggcatgat gatccattgt
     1321 ggcaatccta tccaatatgc ctcggttaat agcccttact ggacagagca tttctatgcc
     1381 tatggacgat taaattaagg agggatttta tgagtaagaa aaaacaaaaa gtgatttcag
     1441 atattaagaa gacaaaggaa aaaatatctg atttacaaag taagcttagg caacttgaaa
     1501 atcagaaaac agaacttgaa aacacggaaa ttgtggaact tgtccgtggt acaaaaatga
     1561 ataccagtga acttgctata ttttta
//
LOCUS       DATGSI010000164         1804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_107444, whole genome shotgun sequence.
ACCESSION   DATGSI010000164 DATGSI010000000
VERSION     DATGSI010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107444"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VK031_02605"
     CDS             complement(<1..273)
                     /locus_tag="VK031_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HLS52834.1"
                     /translation="MGLNIVLVEPEIPHNTGSIARTCALTNTCLHLVKPLGFSVEDKY
                     LKRAGLDYWDLVEIYYYDSFSEILDKYPKNDFYYASTKSKKYYTEFN"
     gene            complement(274..1101)
                     /locus_tag="VK031_02610"
     CDS             complement(274..1101)
                     /locus_tag="VK031_02610"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HLS52835.1"
                     /translation="MDYKLVAIDMDGTLLNNENDISERNRRAIEKAGDKGVYIVLATG
                     RILKSALSYSRRLNLNKPIISCNGAVISDADGKIIYESLLDRETTRKLIEIGNKNNIY
                     YHIYTEDAFYSNVYVEEVLKFYQIGDSDNSQIDYHLFEDPEEIINGDLNIYKFFFLDQ
                     DEKKLEILRNELLELDNISISSSWSNNVEVMGKNTSKGHSLKYLCERLNISQEEVIAI
                     GDNENDLPMINYAGMGVAMDNAIDKVKAVADIKTLKNDEDGVARIIEKYILGEGDEI"
     gene            complement(1257..>1804)
                     /locus_tag="VK031_02615"
     CDS             complement(1257..>1804)
                     /locus_tag="VK031_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS52836.1"
                     /translation="VPQDTVIDYDFTVEEIVMMGRYPYKGRFERESDKDRSITKEVLE
                     LTNTYYLRKRPITEISGGERQRVIIARALAQEPSIILLDEPTSNLDINHQIEILNLLK
                     DLNRNKKTTVVLVIHDINLAARYSDEIILLHEGRVLGQGSPEKIITKSKIEKVYKMEV
                     AIEKNKFTKAIYVTPLGRNSD"
BASE COUNT          540 a          370 c          217 g          677 t
ORIGIN      
        1 gttaaattct gtatagtatt tttttgattt tgtgctggca taataaaagt catttttagg
       61 atatttatct aaaatttcag aaaagctatc ataataataa atctccacca agtcccaata
      121 atcaaggcct gcccttttta aatacttatc ttccaccgaa aaacctaaag gcttcaccag
      181 atggaggcaa gtattggtta aggcacatgt cctggcaatg cttcctgtat tatggggtat
      241 ttctggttca actaatacaa tatttaggcc catttaaatt tcatcacctt cgcctaagat
      301 atacttttct attatcctgg ctacaccatc ttcatcgttt tttaaggttt ttatatctgc
      361 cacagccttt accttatcaa tagcattgtc catggccact cccataccag cataattaat
      421 cataggtaga tcattttcat tatctcctat tgctattacc tcttcctgag agatatttaa
      481 tctttcacaa agatatttta aactatgccc cttagatgta ttcttaccca taacttccac
      541 attattactc caggaactgc ttatagagat attatccaat tctaataatt catttcttaa
      601 aatttctaat ttcttttcat cctgatctaa aaagaaaaat ttataaatat ttaaatctcc
      661 atttattatt tcttctggat cttcaaatag gtggtaatca atctgagaat tgtcagagtc
      721 ccctatttga taaaatttta aaacttcctc tacatataca ttagagtaaa atgcatcttc
      781 tgtataaata tgataatata tattattttt gttgcctatt tctattagct tccttgtggt
      841 ttctctatcc agcaggcttt catatataat tttgccatct gcatcactta taactgcacc
      901 attacatgag ataattggct tatttagatt taacctacgt gaataactta aagcagactt
      961 taaaattctc ccagtggcca agacaatgta aactccctta tctccggcct tttcaatagc
     1021 ccttctgttt ctttcactta tatcattttc attgttcaaa agggtcccat ccatatctat
     1081 tgcaaccaac ttataatcca aaaaaataca tcctcctttc tatatgtaca agcataatat
     1141 atcatattat tagcaaaatg aaaatacctt tttaagcctt gtaataatgt aaaatacaca
     1201 tttaaggcag aaaacgattt ccctttagtt tacaaaaaaa gaacccaaac ttttccctag
     1261 tctgagttcc tgccaagtgg agtgacgtat atggccttgg taaatttgtt tttctcaatg
     1321 gccacttcca ttttatatac cttttctatt tttgatttag taataatctt ttccggacta
     1381 ccttgtccta atactctgcc ctcatgcaag agaattatct catctgaata cctggctgcc
     1441 aaattaatat catgaataac taatactact gtagtttttt tatttctatt taaatctttc
     1501 aaaaggttta aaatttctat ctgatggttt atatccaaat ttgaagtagg ctcatctaac
     1561 aatataattg atggctcctg ggccagggct cttgcaataa ttaccctctg cctttctcca
     1621 ccagaaattt ctgtaattgg cctttttctt aaatagtagg tattggtcaa ttctaggacc
     1681 tcttttgtta ttgacctatc cttatcactt tccctttcaa atcttccttt atatggatat
     1741 cgccccatca tgactatctc ttccacagta aaatcatagt ctattacagt atcctggggt
     1801 acta
//
LOCUS       DATGSI010000165         4309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_202557, whole genome shotgun sequence.
ACCESSION   DATGSI010000165 DATGSI010000000
VERSION     DATGSI010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202557"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VK031_02620"
     CDS             <1..662
                     /locus_tag="VK031_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HLS52837.1"
                     /translation="PDKLPTTIATVMGKIITDTPIEPREGILQHHEGVDLLPANIELS
                     GMEVSLVNAMSRETILKQYLDTMKKQYDFILLDCMPSLGMLTINALAASDSVIIPVQA
                     DYLPAKGLEQLLQTIGKVRRQINPKLKIDGILLTMVDGRTNFAKDISSLVRETYEGSI
                     KVFKADIPRSVKAAEISAEGKSIFAHDPKGKVAEGYGNLTKEVLKIEKLRQKHKAGIS
                     R"
     gene            619..1557
                     /locus_tag="VK031_02625"
     CDS             619..1557
                     /locus_tag="VK031_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HLS52838.1"
                     /translation="MKSSAKNIKLASVDDLFSTEESRADETREKIIEIPLTELHPFKD
                     HPFKVIDNEAMFDTTESVKQYGVLVPAIARPRDEGGYELVAGHRRKRACELAELETMP
                     VIVRSLDDDAAIIIMVDSNLQRENILPSERAFAYKMKLEAMKRQAGRPSKENGSQVGN
                     NLLGKKSSDILAEQIGESKNQIYRYIRLTHLIPELLSMVDEKKIAFNPAVELSFLKNE
                     EQADLLEAMDMEQATPSLSQAQRLKKFSNEGKLSLEVMSAIMSEEKKGDLDKVTLTGD
                     KLKRYFPKSYTPKQMEETIIKLLEGWHKKRTRDQER"
     gene            1590..2534
                     /locus_tag="VK031_02630"
     CDS             1590..2534
                     /locus_tag="VK031_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HLS52839.1"
                     /translation="MAVFRVKKTRDYTVMSNHHLRNTDLSLKAKGLLSLMLSLPDNWD
                     YTTKGLACICKDGIDSINSGVKELEANGYVIRRRLRNEKGQLTTTEYTIFEQPQTLDM
                     TDTPPKGENPILDNPILDNQAQEKPILENPILGKPKQAEPILGNPHQLSTNILNTNSL
                     NTDLLNMEVSNPYPSNLYQSNKEPQEKKMRYDEIGCDSPAEIKEMVLENIEYRYIKDN
                     HNRERLDEIVDLMVETLCSTKDTINVAGDDYPAQLVKEKLLRINCLHIDYVFECLDKT
                     TTYIRNIRRYFLATLFNAPSTIDSYYSALVNHDLNRNS"
     gene            2610..3086
                     /locus_tag="VK031_02635"
     CDS             2610..3086
                     /locus_tag="VK031_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HLS52840.1"
                     /translation="MQEEVENRTVALVISGTKLTARSLKAAILKYLAWRKDKKKYPEI
                     PQGKQSLKDLAKQNAGMTNIEITDKNIKSFERSARKYGVDFAIKKDKSINPPKYLVFF
                     KGRDADAITAAFTDFTSKMVKRAERPSVLSQLKKFTQLVKNTVTDRVKSKDREQSL"
     gene            3083..>4309
                     /locus_tag="VK031_02640"
     CDS             3083..>4309
                     /locus_tag="VK031_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HLS52841.1"
                     /translation="MNTRLKKILILNIPYLFLALFFTKVPQAWRLAFGYDIGQKILNL
                     MNGFRLAFDNPLPSLNPKDLLFAIAIAVIIRLTVYLKGKNAKKYRKGIEYGSARWGNR
                     EDIRPFQDPVFQNNVILTETERLMMSNRPKNPKNARNKNVLIVGGSGSGKTRFWLKPN
                     LMQCHSSYVVTDPKGSIVVECGKLLQRKGYKIKILNTINFKKSMHYNPFAYIKSEKDI
                     LKLVTTLIANTKGDGKGGDDFWVKAETLLYTALIGYIYFEAPEHEQNFSTLIEFINAS
                     EVREDDEDFKNPVDLMFDALEEKDPEHFAVRQYKKYKLAAGKTAKSILISCGARLAPF
                     DIRELREITAYDEMELDTLGDRKTALFIIISDTDDTFNFLVSMAYTQLFNLLCDKADD
                     VYGGRLPIHVRCLIDEA"
BASE COUNT         1573 a          702 c          867 g         1167 t
ORIGIN      
        1 agccggataa gcttccaaca accattgcaa cagtaatggg aaagattatt accgatacac
       61 ccattgagcc tagagaaggc attttacaac atcatgaggg ggtggactta cttccagcaa
      121 acattgaatt atctggtatg gaagtatccc ttgttaatgc tatgagcagg gaaactatac
      181 ttaaacaata ccttgatacg atgaagaagc agtatgattt tatactttta gattgtatgc
      241 cctctttagg tatgttaacg attaatgccc ttgcagcatc tgatagtgta attattcctg
      301 tacaagctga ctatctacct gcaaagggat tagaacagct tttacagacc attggaaagg
      361 tacgcagaca gataaaccct aaactaaaaa ttgatggtat tttgctaact atggtagatg
      421 ggcgtactaa ttttgcaaag gacattagca gtttggtaag agaaacttac gaaggcagta
      481 ttaaagtatt taaagcagat ataccccgct cagtaaaggc agcagaaatc agtgcagagg
      541 gaaaaagtat ttttgcccat gacccaaaag gtaaagttgc agaagggtat ggaaatttaa
      601 ccaaggaggt gttgaaaatt gaaaagctcc gccaaaaaca taaagctggc atcagtagat
      661 gatttattct ccacagagga aagtagggca gatgaaacaa gggaaaagat aattgaaatt
      721 cctttaacag aattacatcc ctttaaagat cacccattta aggtaattga caatgaggct
      781 atgtttgaca caacagaaag tgttaaacaa tatggagttt tagttccagc cattgcaaga
      841 ccccgtgatg agggtggata cgaacttgtg gcgggacacc gaagaaaaag agcctgtgaa
      901 cttgcagaac tagaaacgat gcctgtcata gtccgtagcc ttgacgatga tgccgctatt
      961 attatcatgg ttgacagtaa cttgcaaaga gaaaatattt taccaagtga aagagccttt
     1021 gcatacaaaa tgaaattaga agctatgaaa agacaagctg gtagaccatc aaaagaaaat
     1081 ggttcccaag ttgggaataa tttattaggg aagaaatcaa gcgacatatt agccgagcaa
     1141 attggcgaaa gtaaaaatca aatttatcgt tacatccgtc ttacccatct tatccccgaa
     1201 ctgctttcaa tggtagatga aaagaaaata gcctttaatc ctgctgtgga actatccttc
     1261 cttaaaaatg aggaacaagc tgatttactt gaagctatgg atatggaaca ggcaacacct
     1321 tcactttcac aggcacagag attaaagaaa tttagtaatg aggggaagtt aagccttgaa
     1381 gttatgagtg ccataatgag tgaggaaaag aagggagact tagacaaggt aacccttaca
     1441 ggagataagt taaagaggta ttttccaaag tcatatacac caaaacaaat ggaagaaacg
     1501 attattaaat tacttgaagg ttggcataaa aaacgaacta gggatcagga aagataaaca
     1561 agatataagc aggaaaagag gtgataatta tggcagtttt tagggtaaaa aaaacacggg
     1621 attatacagt aatgtcaaac catcatttaa gaaatacaga cctttcccta aaagcaaaag
     1681 gcttgttatc ccttatgctt tctttaccag acaactggga ttatacgact aagggacttg
     1741 cctgtatatg taaggatggc atagatagta ttaacagtgg ggtaaaggaa ctggaggcta
     1801 atggttatgt aattcgtagg cggttaagaa atgaaaaagg gcaactaact actacagaat
     1861 atactatttt tgaacagcct caaacccttg atatgactga tacaccacct aaaggggaaa
     1921 atccaatact ggataatcct atcttggaca accaagcaca ggagaaacct atattggaaa
     1981 atccaatctt gggaaaacct aagcaggctg agcctatctt gggaaacccc caccaattaa
     2041 gtactaatat attaaatact aattcattaa atactgattt attaaatatg gaggtatcaa
     2101 atccttatcc atcaaatctg tatcaatcaa ataaagagcc gcaagaaaaa aagatgagat
     2161 atgatgagat tggatgtgat agcccggcag aaataaaaga aatggtttta gaaaatattg
     2221 aatataggta tattaaagat aatcataatc gtgagcgttt agatgaaatc gtagacctaa
     2281 tggtagaaac tctatgttct actaaagata ccatcaatgt tgcaggggat gattaccctg
     2341 cacagcttgt aaaagaaaag ctactaagga taaattgtct gcatattgac tatgtctttg
     2401 aatgccttga taagaccacc acctatatcc gtaacatcag gaggtatttt ctggctacct
     2461 tatttaatgc accatccacc atagatagtt attactcggc attggtaaat catgatttaa
     2521 atagaaactc ctaaaggcat tttgtacctg atacaagagg attacaaaat acctttttct
     2581 atggcaaaaa cagaagggag gagcttccta tgcaggaaga agtagaaaat agaacagtag
     2641 cacttgtaat aagcggtaca aagcttacag caagaagttt aaaggcagct attttaaaat
     2701 atctagcatg gcgtaaggat aagaaaaaat accctgaaat tcctcaaggc aagcaaagtc
     2761 ttaaagacct tgccaaacaa aatgcaggaa tgaccaatat tgaaattaca gataaaaata
     2821 ttaaaagctt tgaaagatca gcaagaaaat atggtgtaga ttttgccatt aagaaagata
     2881 aaagtataaa cccacctaaa tatctggtct tttttaaagg aagggatgca gatgccatta
     2941 ctgctgcatt tacggatttt acatctaaaa tggtaaaaag agcagaaagg ccttccgtac
     3001 tttcacagct taaaaagttt acccagcttg tgaaaaacac agttacagat agggtgaaaa
     3061 gtaaagatag ggagcaaagc ctatgaatac aagactaaaa aaaatattaa ttctaaatat
     3121 cccttatcta tttttagccc tattttttac caaggtacca caggcatgga gacttgcctt
     3181 tggatatgat attgggcaga aaattttaaa cctaatgaat ggctttagat tagcatttga
     3241 taacccactt ccaagtttaa acccaaaaga tttactattt gccatagcta tagcggttat
     3301 tattcgcctt acagtttatc taaagggtaa gaatgctaag aaatatagaa aggggataga
     3361 atacggctct gcccgttggg gcaatagaga ggatatccgc ccttttcaag accccgtatt
     3421 ccaaaataac gtgatactaa ctgaaacaga aaggcttatg atgtctaacc gtcctaagaa
     3481 tcctaaaaat gctagaaata aaaatgtgct aatcgtaggt ggttctggct caggaaagac
     3541 aaggttttgg ctaaagccaa atctgatgca atgtcatagt tcatatgtgg ttacagatcc
     3601 aaagggcagt attgttgtgg agtgcgggaa attattacag cgtaagggat ataaaattaa
     3661 gattcttaac accattaatt ttaaaaagtc tatgcactat aatccatttg cctatatcaa
     3721 aagcgaaaag gatattttaa aactggttac tactttgatt gccaatacca aaggtgatgg
     3781 taagggaggt gatgattttt gggtaaaggc agaaaccctg ctttataccg cactcattgg
     3841 ctatatctat tttgaggctc ctgaacatga acaaaacttt tctaccctga ttgaatttat
     3901 caatgcttca gaggtgcgtg aagatgatga ggattttaaa aatcctgtag atttaatgtt
     3961 tgatgcctta gaagaaaaag acccagaaca ttttgcagta aggcagtaca agaaatacaa
     4021 attagcggca ggtaaaactg ctaaatctat tcttatatct tgtggagcaa gactagctcc
     4081 ctttgatatt agggaattaa gagaaattac tgcctatgat gaaatggaac tagatacctt
     4141 gggagataga aaaacagcct tgtttattat cattagtgat acagatgata cctttaactt
     4201 tttagtatct atggcatata cccaactttt taatttactt tgtgataagg cagatgatgt
     4261 atatgggggt agattaccca ttcatgtaag gtgtctaatt gatgaagct
//
LOCUS       DATGSI010000166         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_58510, whole genome shotgun sequence.
ACCESSION   DATGSI010000166 DATGSI010000000
VERSION     DATGSI010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58510"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VK031_02645"
     CDS             complement(<1..544)
                     /locus_tag="VK031_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HLS52842.1"
                     /translation="MRKFQFVSLLLIISLIFTACGQKEEAIDTWKELKDRGTIIVGLD
                     DTFVPMGFRDKDGKLTGFDIDLAREAIERLGLELELQAIDWNLKEQELISGNIDLIWN
                     AYTITDSRLEKVNFTKPYLDNKQLIVVLSDSDIDSKDDLKDKIVCTQNSSSALDAIEK
                     SGAKEDFKDGDLVLFDTYTEA"
     misc_binding    complement(589..838)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            981..1886
                     /locus_tag="VK031_02650"
     CDS             981..1886
                     /locus_tag="VK031_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLS52843.1"
                     /translation="MEKSSNRQVYIGAFIYALIMGHSFLFTKIALESANPMDILAYRF
                     TAAFIGIMIPRISGWIKVKYTRESIRRIIPIAILYPLLFFGFQTTGLQYTTSSEAGIL
                     SAAAPIFTMILASYFLDEKTTTLQRLSIIVSVLGVVYVSVMKGASLDINNILGIVLLI
                     LSTISLAGYSVMGRVLTRDFSNIELSYLMITISFIVYNLIAILNHAINGSVKNFFIPL
                     SSMGFVISILYLGVLSSLVTSLLTNFILSRIEASRMSVFANLGTVITIIAGAVYLKEK
                     IYYYHIIGSILIISGVIGTNYFKEE"
     gene            2065..>2537
                     /locus_tag="VK031_02655"
     CDS             2065..>2537
                     /locus_tag="VK031_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52844.1"
                     /translation="MGSIRKQMLIYLLIGSIVIFSLLFLVFNLKLKSLPEHSREQYEE
                     IVKARADEVSKELKLAIGQICVISNSPIIQSMDLARIKEYLPNLILNSNYKSLTIAYP
                     DGQAWGTKDKEFNIKDQEQYRKIFLENKEYHISQPFRSPFAYEDENEIIVVAHRIV"
BASE COUNT          821 a          440 c          427 g          849 t
ORIGIN      
        1 aggcctcagt ataggtatcg aatagaacca gatcaccatc tttaaaatct tccttggctc
       61 ccgatttttc aatggcatcc aaggcactgg aggagttttg ggtacaaact attttatcct
      121 ttaagtcatc cttgctatct atatcagaat cacttagaac tacaattaat tgcttgttgt
      181 ctaggtaagg ttttgtaaag tttaccttct ccagcctgct gtcagtaata gtatacgcat
      241 tccatattag atcaatatta cctgagataa gttcctgttc cttaagattc caatctatgg
      301 cctgtaattc taattcaagg cctaaccttt caatggcctc cctggccaaa tcaatatcaa
      361 agcctgttaa cttaccatct ttatctctaa aacccatagg gacaaaggta tcatcaagcc
      421 ccactataat ggtgccccta tccttaagct ccttccaagt atctattgct tcttcctttt
      481 gtccacaagc tgtaaaaatc aggctaataa ttaataacaa actaacaaat tgaaactttc
      541 tcattttcaa tacctccaaa atttttattt aaaaaagctt tcgtccaaga ataggacgaa
      601 agctttcgct tacgtggttc cacctaattt tactggtacc tcacgatacc agcctcagta
      661 agtatgctaa cacatactaa tcatggtaac ggatgattcc cccgagaaaa cctactggat
      721 agctttcagt tttctagctc aggaatgtgt tctaaatggc ttttatgtac cttctcacca
      781 aaagggtact ctctgtaata aaagtgacat ttatactatt ttccttcatc gcctacttta
      841 ctatgatact ataataaagt atatttcatt aaatgtcaat agttatttta atttctttcc
      901 aacagaccag gcttaattaa agcacaaaga cttatttgtg atataatgct aattgtaaat
      961 atgaaaggta gttgattata atggaaaaat caagtaacag gcaagtatac ataggagctt
     1021 ttatatatgc cttgataatg ggacattctt ttctctttac aaaaatcgct ttggaaagtg
     1081 ctaaccctat ggatatattg gcctataggt tcacagcggc ctttatcgga ataatgattc
     1141 caagaatctc tggttggatc aaggttaaat atactaggga aagtattagg cgaattatac
     1201 ctatagcaat tttgtatccc ttattattct ttggattcca gactacaggc ctacaataca
     1261 caacctcatc agaggcaggg atcctgtctg cggcagcccc catatttaca atgattttag
     1321 cctcttattt cctagatgaa aaaaccacaa cccttcaaag attatctata atcgtatcag
     1381 ttctgggtgt agtgtatgta agtgtaatga aaggggcaag tctagatatt aataatattt
     1441 taggcatagt tttattgatt ttgtccacca tatccttagc tggttatagt gtgatggggc
     1501 gagttttgac cagggatttt tccaatatag agcttagtta tttaatgatt accataagct
     1561 ttattgtcta taacctgatt gccatattga accacgccat aaatgggagc gttaaaaatt
     1621 tctttattcc attatcaagt atgggatttg ttatttccat actgtatttg ggagttttat
     1681 cttccctggt tacctctcta ttaaccaatt ttattttatc taggattgaa gcctctagaa
     1741 tgagcgtatt tgctaattta ggtactgtaa taactattat tgcaggggca gtttatctaa
     1801 aagaaaaaat ttactactat catataatag gctccatatt aataataagt ggagtaatag
     1861 gaaccaacta ttttaaggaa gaataaactt gtggctaggc ccaatatttt aggaggcatg
     1921 gtggtggatt tgttaaaaaa gtagcaaaag actataaaag attgctacca tctgtcgata
     1981 tacagtatta gaattataaa ttatgattta aattaatttt ctacataagc agggccaaca
     2041 aatgacaaat aaggtgggat taatatggga tccatcagga agcaaatgtt aatttatctt
     2101 ttaataggta gtatagttat atttagcctt ctttttctag tttttaattt gaagttaaaa
     2161 agcttacctg aacacagtag agaacaatat gaagaaattg ttaaagcaag ggccgatgaa
     2221 gtgagtaagg aacttaagtt agctattgga caaatatgtg ttatttcaaa ttcacctata
     2281 attcagtcta tggatttagc taggattaag gaatatctac ctaatttaat actaaatagc
     2341 aattataaaa gcttaacaat agcttatcct gatggtcaag cctggggaac taaagataag
     2401 gagtttaata ttaaagacca ggaacagtat aggaagattt ttcttgaaaa taaggagtat
     2461 catatatccc aaccctttcg tagtcccttt gcttacgaag atgaaaatga aattattgtg
     2521 gttgctcaca ggattgt
//
LOCUS       DATGSI010000167         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_202940, whole genome shotgun sequence.
ACCESSION   DATGSI010000167 DATGSI010000000
VERSION     DATGSI010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202940"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..388
                     /locus_tag="VK031_02660"
     CDS             173..388
                     /locus_tag="VK031_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52845.1"
                     /translation="MTDNDRIIRDLLIELGKRREEDEQHIKNRIEELKNKIIESKPYN
                     IDLEFTLDLLIDYSKVLGVYESSSGER"
     gene            498..890
                     /locus_tag="VK031_02665"
     CDS             498..890
                     /locus_tag="VK031_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52846.1"
                     /translation="MEDLDKVTKLKETVRRLLNPLLGELGKVRKYDSGGAIVRGEPRI
                     YVNKTTYTLGEISVIKEEVSGKVSLYRTITVLVKGYQYINIGSENPQYLKLEGVFEDL
                     EHKYSEQLEEIPESTPEDIMERYLTHLK"
BASE COUNT          428 a          117 c          232 g          277 t
ORIGIN      
        1 tgataaagga attaggataa gagatgttaa gctgactttg aaagggatgc ataacaaaag
       61 tctttttata gcaggtgacg atgtaaatgg tataacagac aattacttag aagagtacga
      121 agagtatata gactacttag tagaaggtat aacattttaa aacccagtaa agatgacaga
      181 caacgataga ataatacggg atttacttat tgaacttggt aagagacgtg aagaggatga
      241 gcaacatata aaaaacagaa tagaggaact taagaataag ataatagaga gtaaacctta
      301 caacatagac ttagagttca cactagacct tttaatagat tattccaagg tattaggggt
      361 gtacgagagt tcttcgggag agcgttaaag gtagtattac tactttaata ccgataaatt
      421 taaacaaatg agaataatat gacaaaagac ggctgtaaaa tagctacctt tgttttagaa
      481 aaaaaaataa gaaagatatg gaagatttag ataaagtaac gaaattaaaa gagacggttc
      541 gtagattgct gaatccgtta ttaggagagt tggggaaagt aagaaagtat gattcagggg
      601 gcgctattgt acgtggagag cctagaatat atgtcaacaa aacaacctat acactaggag
      661 agattagtgt cattaaggaa gaggtttcag ggaaggttag tctctataga acaataacgg
      721 tattagttaa aggataccag tatatcaata tagggtcaga aaacccacaa tatctgaaac
      781 tagaaggggt ttttgaagac cttgaacata agtattcgga acaattggag gagataccag
      841 aaagtacacc agaggatata atggagaggt atctaactca tcttaaataa aaatagcgtt
      901 taaaatatgt taaaaatgaa aaaactatta aaaaatatta aatatctgtt caaacctaag
      961 ttttggatga tgttataccc atacagcagg gcatgggata ttaagttaaa tgaaatgctt
     1021 gatagagaga atagtgttga ggttgtaagt gagt
//
LOCUS       DATGSI010000168         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_246393, whole genome shotgun sequence.
ACCESSION   DATGSI010000168 DATGSI010000000
VERSION     DATGSI010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246393"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..953
                     /locus_tag="VK031_02670"
     CDS             165..953
                     /locus_tag="VK031_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52847.1"
                     /translation="MATKTLLDIIQNVMIAMGDDPINSISDSPESEDLDVMAKGVFEE
                     IITWDEWPDTFKIASLESVSDYNCPVALRINDNYRYIESVEYFIEDRNTFRTLDYYTP
                     PRFLDATNWYFNDTHHIKVKGYGIDSSFVPVLNNKDPQYYTTFDDKHIITNSYDYERE
                     SNLQESNCRVWVCVVPDFKIEDNFILNYQQDLINLFISELKVAASYYMNQQDNPRDRK
                     QTLRILNKLRRYKGKVINEQEESWPNGVYGRNGRSKNIRKMGRR"
     gene            895..1167
                     /locus_tag="VK031_02675"
     CDS             895..1167
                     /locus_tag="VK031_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52848.1"
                     /translation="MVYTDETEDQRTSEKWVAGKTKSGKDLVVKPDGRGLFYVEQIGG
                     GKKAKEFSGKFTGYMSAQKAVDAYRIKTNYAYRMKKTEKKEKKVDA"
     gene            1160..>3250
                     /locus_tag="VK031_02680"
     CDS             1160..>3250
                     /locus_tag="VK031_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52849.1"
                     /translation="MPRARQEVTSATFAKGIITETGPLGYPDNAVKDMNNLVLERNGS
                     VHVRNGIVLDNFGAIQDINKGDTYSIHYWKDPAGDGENFIVLQIGHFIKCLSTRGDHI
                     GKKQYTASTLSNGQEVTDLTDIFNFGGQIHDLDSVQSKNRLYMTSKETSCFYLEYLPE
                     GRLNSDSYSDYPGATADPTVQITSIDLKYRDLYGIHDGFKVDERPTSPDNAHRYNLLN
                     SGWKKEHINKFYAGRTGHDEPDWPGGKYPANADYYWFGWGINPYGEGDVADHFLPGRY
                     YEYPISTTPAPKGKFILNVLNPDREKVSGVGNLPKRGKAAGPTSIGFYAGRVWYAGFS
                     GGGFDDNILFSNVLPFDDLVEYKDDQFYDVYAGQCYQKNDPSARDVNDLVDDDGGIIP
                     IPGLGKVQGITSLSSSLLVISDQGAWAISGGIDPFSANNYSVNKITDIGCVSPTSITE
                     MGGSIAYWGLGSIVVIGQNQMGSTQAESATYQTIESIYSRIPLDEKRTAHGTYDDISK
                     KVYWTYSQNKCLIFDVQLGAFSRYTFTDEIEGIVLLPTINDTVQHLHNNVVLHGSQVT
                     LNGEDVGFEEEIPVDADTRVLPIIKKDNYIIPCIFAPEDSGIYYDLLPPEYGSLGYDL
                     YPVHAFLEVGATNFGDIMRRKQSYRALCYFESMESLAIPTDYSVANGEMSADNLNFIQ
                     ADESGCWVQARWDWS"
BASE COUNT         1035 a          581 c          727 g          907 t
ORIGIN      
        1 caggtgaaat ctgacagcgg aacatggcaa gatatgaaag gaagctcttt tatttctcta
       61 tttaatctat ccggaggaga aggcatcgaa gtcagagatg cccatgaagg cggtgtgact
      121 cagatatctc ctgatctcag aataggaata gtacgagtaa tgtaatggct actaaaactc
      181 tactggatat tattcagaat gtaatgatag ctatgggaga cgatcctata aatagtatat
      241 ctgacagtcc cgaatcagaa gacctcgacg ttatggccaa gggagtcttt gaagaaataa
      301 ttacatggga cgaatggcct gatacattta aaatagcatc attagaatct gtatctgatt
      361 acaactgccc cgtagccctt aggattaacg ataattacag atacatagaa tcagttgaat
      421 actttatcga agatagaaat accttcagaa ctctggatta ttacactcct cctagatttt
      481 tagatgccac taactggtat ttcaatgaca ctcatcatat aaaagtaaag ggatatggta
      541 tagatagttc ttttgttccg gttctgaata ataaagatcc ccagtattat actacctttg
      601 acgataaaca tattataact aattcttatg attatgaaag ggagtctaat ctccaagaaa
      661 gtaattgcag ggtatgggtt tgtgttgtac ctgacttcaa gatagaggat aacttcattc
      721 ttaactatca acaggacttg ataaacttat tcatatcaga gttgaaagtg gctgcgtcct
      781 attatatgaa ccagcaagac aatcctaggg atagaaagca gactctaagg attcttaata
      841 agctacgtag atataaagga aaagtaatta atgagcaaga agaaagctgg ccgaatggtg
      901 tatacggacg aaacggaaga tcaaagaaca tcagaaaaat gggtcgcagg taaaactaaa
      961 tccggaaaag accttgtagt taagccagat ggtaggggtc tattctatgt agaacaaatt
     1021 ggtggtggca agaaagccaa agaattctct ggtaaattta ctggatacat gtcagcccag
     1081 aaagctgtag atgcctacag gataaaaacc aattatgcct accgtatgaa aaagacagaa
     1141 aagaaagaga agaaggtaga tgcctagagc aagacaagaa gttactagtg caacctttgc
     1201 taaagggatt atcacagaga caggtcctct gggatatcct gataatgcag taaaggacat
     1261 gaacaattta gtgctagagc gtaatggttc ggtgcatgtt cgtaatggta ttgtgctgga
     1321 taactttggt gctattcagg atataaataa aggagataca tactctatcc attactggaa
     1381 agacccagca ggagatggtg agaactttat tgttttacag ataggacact tcattaagtg
     1441 tctatctact agaggagacc atataggtaa gaagcagtac actgccagta ctctaagcaa
     1501 cggccaagaa gtaactgatc ttactgatat atttaacttc ggcggccaaa tacatgacct
     1561 tgattctgta cagtccaaga atagattgta catgaccagt aaagaaacta gctgtttcta
     1621 tctggaatat ttgcctgaag gcagattaaa ctctgatagt tattctgatt atccgggagc
     1681 tactgctgac cctactgtac agattacttc tattgattta aagtatagag acttatatgg
     1741 tatacatgat ggattcaaag tagacgaacg tcctactagt ccagacaatg ctcacagata
     1801 caatcttcta aactctggat ggaagaaaga acacatcaat aaattctatg ccggtaggac
     1861 tgggcacgac gaacccgact ggcccggtgg gaagtatccc gctaatgccg actattactg
     1921 gtttggctgg ggtattaatc cttatgggga gggagatgtc gcagaccatt ttcttccggg
     1981 aaggtactat gaatatccaa tcagcactac tcctgctcct aaaggaaagt tcattcttaa
     2041 tgtgcttaat ccagatagag agaaagtatc gggagtgggc aatcttccca agagaggaaa
     2101 ggctgccggt cctacttcaa taggattcta cgctggtagg gtgtggtatg ccggattctc
     2161 cggaggaggt tttgatgata atattctatt cagtaatgta cttccttttg atgacttagt
     2221 agagtataag gacgaccagt tctatgatgt gtacgctggt cagtgctacc aaaagaacga
     2281 tccctccgct agagatgtaa atgatctagt agacgatgat gggggaatta ttcctattcc
     2341 cggactaggt aaagtacagg gcattacgtc tctttcaagt tctttgcttg ttattagtga
     2401 tcagggggcg tgggcgattt ctggaggcat agatcccttt agtgctaata attattctgt
     2461 taataagatt acggatatag gctgtgtatc tcctacttcg attaccgaga tgggagggag
     2521 cattgcttat tggggcctag gtagtatagt cgttattggt caaaaccaaa tggggtctac
     2581 tcaagcagag agtgctactt atcaaaccat tgagtctatc tattctcgta tacctttaga
     2641 tgaaaagaga actgcacacg gaacttatga tgatatctct aaaaaggtgt actggacata
     2701 tagccaaaat aaatgtttaa tctttgatgt tcagcttggg gcattctcaa gatatacttt
     2761 cacagatgag atagaaggaa ttgtacttct tcctactatc aatgatactg tacaacatct
     2821 ccataacaat gtagttctac acggaagtca agttacactc aatggggaag acgttggatt
     2881 cgaggaggag attccagtag atgcggatac tagggttcta cctattatca aaaaggataa
     2941 ctatattatc ccatgtatat ttgcccctga agatagcggc atctattatg atttacttcc
     3001 gccagagtat gggtctctag gatatgatct ataccctgtt cacgcattcc ttgaggtagg
     3061 cgcaactaac ttcggggata tcatgagaag gaagcaatcc taccgagcgc tgtgctattt
     3121 cgaatccatg gagtctcttg ctattcccac ggattactca gtagctaatg gagaaatgtc
     3181 agccgacaac cttaatttca tacaagcaga cgaatcagga tgttgggtac aggctagatg
     3241 ggattggtcc
//
LOCUS       DATGSI010000169         1104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_532400, whole genome shotgun sequence.
ACCESSION   DATGSI010000169 DATGSI010000000
VERSION     DATGSI010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532400"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1104
                     /gene="dnaE"
                     /locus_tag="VK031_02685"
     CDS             <1..>1104
                     /gene="dnaE"
                     /locus_tag="VK031_02685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HLS52850.1"
                     /translation="GYQNLMKLVSEAYINGFYYKPRVDHNLLAKYSEGIIALSGCLSG
                     EVQRFLLDDNYEKAREVALKYKSIFGKDNFFLEVQDHGLKEQVKINQGLVKLSQELDL
                     SLVATNDVHYLNQEDSVIHDVLLCIQTGKTIDDTDRMKFPTDQFYFKSAEEMAEIFRN
                     YQGALENTVKIAERCNVELDFDTLHLPEYKVPEGYTNESYLKELTLSGLKERYGSLSQ
                     EVMDRFQYEFGTITDMGYTDYFLIVWDFIKFAKDNDIMVGPGRGSAAGSIISYGLGII
                     DIDPLKYGLIFERFLNPERVSMPDIDIDFCYERREEVIEYVIKKYGADRVAQIVTFGT
                     MAARGAIRDVGRAINMPYKDVDYVAKQIPTDLG"
BASE COUNT          369 a          152 c          248 g          335 t
ORIGIN      
        1 aaggttacca aaacttaatg aagctagttt ctgaagcata tattaatgga ttttattata
       61 agcctagggt agatcataat ctattggcta aatacagtga gggaattata gccttgtctg
      121 gttgcctttc tggcgaagtc caacgatttt tgctagatga taattacgag aaagccaggg
      181 aagtggccct gaagtataaa agtatatttg gtaaagacaa ttttttccta gaagttcagg
      241 accatggcct aaaagagcag gttaaaatca atcagggctt ggtgaaacta agtcaagagc
      301 tggatttatc tttggtagcc accaatgatg tccattatct gaatcaagaa gattctgtaa
      361 ttcatgatgt tttattatgt atccagactg gtaaaactat agatgatact gatagaatga
      421 agtttcctac agaccaattt tattttaaaa gtgctgagga aatggcagaa atatttagaa
      481 actatcaggg agcattggaa aacacagtca aaattgcaga aagatgtaat gtggaattgg
      541 actttgacac cttacaccta cctgaataca aggttccaga aggttatacc aatgaatctt
      601 atctaaaaga attaaccctt tcagggctta aggaaaggta tggaagttta agccaggaag
      661 taatggatag gttccaatat gaatttggaa ctattactga tatggggtat actgactact
      721 ttcttatagt ctgggatttt attaaatttg ctaaagataa tgatataatg gtagggccag
      781 gtagagggtc tgcagccggt agcatcatat cctatggttt aggaattata gacattgatc
      841 ctttaaagta tggattaatc tttgaaagat ttttaaatcc ggaacgggtt tccatgcctg
      901 atatagatat agatttttgt tatgaaagac gggaagaagt cattgaatat gtaataaaaa
      961 aatatggggc tgatagggta gcccaaatag ttacctttgg tactatggct gccagggggg
     1021 ctattaggga tgtgggaagg gctattaata tgccttacaa agatgtggac tatgtggcca
     1081 aacaaattcc tacagattta ggta
//
LOCUS       DATGSI010000170         3668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_485711, whole genome shotgun sequence.
ACCESSION   DATGSI010000170 DATGSI010000000
VERSION     DATGSI010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485711"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VK031_02690"
     CDS             complement(<1..406)
                     /locus_tag="VK031_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52851.1"
                     /translation="MIESRDKRFPSPYVSNEVKSTKEYGRQVFEAVMANSGSYKNSIL
                     KKKGEARRYSRGEQPLQRLLEEINIEGNSQVINVSYKPTKILQNFERVVVDDYAQLKE
                     SIGVEATSSLIKIRKEKKKSDLKFNFEYKELLQ"
     gene            complement(476..2611)
                     /locus_tag="VK031_02695"
     CDS             complement(476..2611)
                     /locus_tag="VK031_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52852.1"
                     /translation="MSNKDYVISKEQQKLDRIINKNSVQLGIWDKGNLLIEGLKYEFP
                     KKPNKKDFINYGKPKSEQYWQRSTEYEKWDWNLDDSKGQPLWYDAPAEGQLEWYLEEI
                     DRFVNGVWILIDGEEVYFNKYTYFFLQWFLLEDGTYPNFRDSILYYYRFIEICERSPV
                     CIGHTLLKSRRMGATSMCLSALLLLAITTDNSNYGILSNKGDNAKKAFQRAVKAFGRI
                     PPFLRPVQEGTTAPKKILSLREQAKRITKSTSRGEAQEGLNNELSWENTDLNSYDSYA
                     LAAVLADELSKYPREVPVNEYLDVLKECCKKGTRIVGKILGPTTVNPPDKGGSNYKIV
                     WDGSDQTSKGAETGTQSGLYRIMIPSYIGLEGYVTKYGQSIVDKPTKKQTELLKEVGC
                     PNPNIGARQFLLDDRAKSENNTDSLQAKIRQYPFNAQEVFDASNSGCIFPNREQLIER
                     RKVLEDKLAELGLTSDKDELGRRGWFHRVNGVIQFIDDSKNGLWYVDKLLPDNQANKY
                     SYDRSNRKKPENEEWGAGGADPIASGDAPLDGGSDACLIIRSRFSSLDPDNTGYPVAM
                     FLGRMDNPNKMHEQWYNALEYYGVRMLAERAPQNWIDYAEAHKLQNYLYGTVRSDGKV
                     IKGVNSQGEAIKQEHAETQVLQALSDVDKVPFVTLIRQRLAFNVRNRTDYDTCMADGY
                     AGMALKIPFKTSKKKEGKKVKFFRRGRIL"
     gene            complement(2613..3059)
                     /locus_tag="VK031_02700"
     CDS             complement(2613..3059)
                     /locus_tag="VK031_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52853.1"
                     /translation="MEKIRIDRTIRSGDDKIDKYIEDLEDYLLSLGATGTNKLILKLE
                     EVSSSITDDLDMILAGNDGDFIENSKGDLVWKSALKILNDSNTSKTFDRVMALYSKIG
                     DIKAVSEAVRDLVPEEKVEGEDDKVAIKSSSPYEEALRIAKGGKKK"
     gene            complement(3133..3435)
                     /locus_tag="VK031_02705"
     CDS             complement(3133..3435)
                     /locus_tag="VK031_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52854.1"
                     /translation="MDVINSNLGFRPIGQRLLVKKPVLKKEETTTSGIVIPQTAKSVS
                     DHMTIAEVLATGDEVTNVRVGDIIKFENPHPVKFKDEEYFLINERNVLLILDEPLT"
     gene            complement(3440..>3668)
                     /locus_tag="VK031_02710"
     CDS             complement(3440..>3668)
                     /locus_tag="VK031_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52855.1"
                     /translation="RDIVKVINPCPDTKVKKGDYLLTQLGGDYEFYNGRELLISVDEY
                     FDDYYAIVDSEHWYDGGDIRRSVNLNEQISF"
BASE COUNT          882 a          843 c          569 g         1374 t
ORIGIN      
        1 cttgtagtag ctctttatat tcaaaattga attttaggtc ggacttcttc ttctctttac
       61 gtatctttat taatgaggaa gtagcttcta cgcctatact ctcttttaat tgagcataat
      121 cgtccaccac aactctctcg aagttttgaa gtatcttagt tggcttatag ctaacattaa
      181 ttacctgact attaccttcg atatttatct cctctaataa tctttgtaaa ggttgctctc
      241 ctcgtgagta ccttctagct tcacccttct tttttagtat ggagttttta taactccccg
      301 agttagccat tactgcttca aatacctgtc tcccgtattc ttttgtactc tttacttcat
      361 tactaacata tggtgaagga aatctcttat ctctactttc aatcattctt tatctctaat
      421 tattcgtcta tgtttacaaa gttatggttt attattagcc cattacgtta agggtctata
      481 gtatcctccc tcgtctaaag aatttaacct ttttaccctc tttcttctta ctagtcttaa
      541 atggaatctt taatgccata cctgcatatc cgtctgccat gcacgtatca tagtctgttc
      601 tgttcctgac attgaaagct aatctctgtc tgattaatgt aacaaatggt actttgtcta
      661 catctgataa tgcctgcaac acttgcgttt cagcgtgttc ttgtttaata gcttcccctt
      721 gagagttaac tcctttaatt acttttccat ctgacctaac tgttccgtat aaatagttct
      781 gaagtttatg agcttctgcg tagtctatcc agttttgtgg cgcacgctct gctaacattc
      841 tgactccata atactctaat gcgttatacc attgctcgtg catcttatta gggttgtcca
      901 tacgacctag gaacatggct actgggtatc ctgtattgtc tgggtctaac gaactaaatc
      961 tgcttctaat aattaaacaa gcgtctgaac ctccatctaa aggtgcgtct cctgaagcga
     1021 tagggtctgc tcctcctgct ccccactcct cgttctctgg cttcttccta ttgcttctgt
     1081 catatgagta tttattggct tggttgtctg gaagtagttt gtctacgtac cataacccat
     1141 tcttagagtc atctataaac tgaataactc cgtttactct atgaaaccac cctcttcttc
     1201 ctaattcatc tttatctgaa gttaacccta attcagctag tttatcctct agtaccttac
     1261 gtctttctat aagttgctcc ctgttaggga atatacatcc actgttagat gcatcaaata
     1321 cttcctgagc attaaacgga tattgtctta tcttagcctg caagctgtct gtattgtttt
     1381 ctgacttagc tctatcgtct agtaagaatt gtctagctcc tatattagga ttagggcaac
     1441 ctacctcttt aagtaattcg gtttgttttt tagtaggctt gtctactatt gattgcccgt
     1501 atttggtcac atacccttca agacctatat aagaaggaat cattattctg tatagtcccg
     1561 actgagtgcc tgtttcagcc cccttactag tctggtctga accgtcccaa actatcttgt
     1621 aattagaccc tcctttatct ggggggttta ctgtggttgg ccctaatatc ttcccaacaa
     1681 ttcttgtacc ctttttacaa cactctttta gtacgtctaa gtattcgtta actggcactt
     1741 ctcttggata cttagaaagc tcatccgcta acactgcggc caaagcatat gagtcataag
     1801 agtttaggtc tgtgttctcc caacttagct cgttgttcag tccttcctgt gcttcacctc
     1861 tgctagtgga ttttgtaata cgtttcgctt gttctcttaa agataaaatc ttctttggtg
     1921 ctgttgttcc ttcctgtact ggtcttagga atgggggaat cctaccaaac gcctttactg
     1981 ctctttggaa agccttctta gcgttatccc ccttgttgct taatatgccg tagttggagt
     2041 tatctgttgt gattgcaagt aagagtagtg ctgataaaca catagaggtg gcccccatac
     2101 gtcttgactt tagtaacgta tgtccgatgc atactggact tctctcgcat atctcaataa
     2161 acctatagta gtatagtata gaatccctga agttagggta agtcccatcc tctaatagaa
     2221 accattgtaa gaaaaagtag gtgtacttat taaagtaaac ctcttcgcca tctataagta
     2281 tccatactcc gttgacaaat cggtctatct cctctaggta ccactccaac tgcccttccg
     2341 caggtgcgtc ataccataat ggttgcccct tagagtcgtc taagttccag tcccatttct
     2401 cgtattcggt actcctttgc caatattgtt cggatttagg ttttccgtaa tttataaagt
     2461 ccttcttgtt gggcttctta gggaactcat atttaaggcc ttctattaat agattcccct
     2521 tatcccatat tcctaactgt acggagttct tatttattat tctgtctaac ttctgttgct
     2581 ctttagagat tacgtaatct ttgttactca tattacttct tcttaccccc tttagctatc
     2641 cgtaaagctt cctcgtaagg actgctcgac ttaatggcta ctttatcatc ctctccctct
     2701 actttctctt caggtactaa atcccttact gcttcagata ctgctttaat gtcgcctatc
     2761 ttagagtata aggccattac cctgtcaaat gttttagagg tgttagagtc attaagtatt
     2821 ttaagtgcgc tcttccaaac caaatctcct tttgagttct ctataaagtc tccatcgtta
     2881 cctgctagaa tcatgtctaa gtcatccgtt atactactag atacttcttc aagtttaagt
     2941 attagtttgt ttgtacctgt agctcctaga cttaataagt agtcttctag gtcttcaatg
     3001 tacttatcta tcttatcatc tccgcttcta atcgttctgt ctattcttat tttctccatc
     3061 tgccctaaaa ataaaaaacc gaacacagac tattccatgc tcggtacaaa actactaaaa
     3121 gctttctatt gtttacgtta agggttcatc aaggattaaa agtacgtttc tttcattaat
     3181 tagaaagtac tcttcgtcct taaatttaac tgggtgtgga ttctcgaatt tgattatgtc
     3241 tcccaccctt acgtttgtta cttcatcccc tgttgctaga acttcagcga ttgtcatatg
     3301 gtcgcttacg ctcttggctg tttgaggaat tactattcca cttgttgttg tttcttcttt
     3361 cttcagaact ggctttttta ctagaagcct ttgaccaata ggtctgaatc ctaaattact
     3421 atttattacg tccatatcct taaaaactta tctgctcatt taaattaaca cttctcctaa
     3481 tatctcctcc gtcataccaa tgttcgctat ctactattgc ataatagtca tcgaaatatt
     3541 catctacgga tattaataac tcccttccat tataaaattc atagtcacct ccaagttggg
     3601 taagtaggta atctcccttt tttacctttg tgtctggaca tgggtttata accttcacta
     3661 tatccctt
//
LOCUS       DATGSI010000171         3333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_391468, whole genome shotgun sequence.
ACCESSION   DATGSI010000171 DATGSI010000000
VERSION     DATGSI010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391468"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VK031_02715"
     CDS             complement(<1..287)
                     /locus_tag="VK031_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52856.1"
                     /translation="MSKPLQLGVHFKRDREAEFISQTWFNFNSQRQAKLEEWKEVRNY
                     VFATDTSTTSNASLPWKNKTTLPKLCQIRDNLYSNYISALFPNDDWLKWEA"
     gene            complement(290..1897)
                     /locus_tag="VK031_02720"
     CDS             complement(290..1897)
                     /locus_tag="VK031_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52857.1"
                     /translation="MRNEWIRKATDRLSRMPEEAKEVRETALTDLYFFARLVNPGYVY
                     GEIHKEVFKWISEYSLFGQGEGLSSNKLIMLPRAHLKSHCVATWVAWIITRHPEVTIL
                     YVSATSELAETQLFSIQNILASDVYQRYFPEYINPQEGKRERWSQRRFSIDHAKRRQE
                     GIRDPTVATAGLTTNTTGWHADIVVADDLVVPENAYTQEGRDSVMKKSSQFSSIRNAG
                     GFTLACGTRYHPSDVYALWKEQAYDIYDESGETVDRVPVWEVKEHTVETDGVFLWPRT
                     VRASDGKFFGFDRQTLSRIEAEYEDRTQFFAQYYNNPNESGSNRISPSCFQYYTPKFI
                     KRKSGNWYFKDKRLNVYASIDFAFSLRKRADYSAIVVIGIDPESNIYVLDIDRFKTDK
                     ISEYYDRIVKLHSRWDFKKLRAETTAAQVIIVRDLKDRMRDRGLSLPIEEYRPNRSEG
                     SKEERIAAALEPKYEDMKMWHFEGGLTPLLEEELLLSRPPHDDMKDALAAVVSIAVRP
                     RDRRSMDGARDNRVVFNSRFGGVAFRG"
     gene            complement(1901..2428)
                     /locus_tag="VK031_02725"
     CDS             complement(1901..2428)
                     /locus_tag="VK031_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52858.1"
                     /translation="MSVTKKQEKLLLPTKDQMLDTSGRPLTQSLFLEFSYSPFAIYTL
                     KDQDHEYNGKIYPSIKKLYMEEDDPTEYRFANKYFLNMRHWLRICNNKLMTDHIEEWR
                     YELELKLRSEGISQMVKAARRGSQGAARFLTDRGWEVRKAGRPSKEELEREAKIQNNI
                     KDEYADDLRRLVAIK"
     gene            complement(2385..2837)
                     /locus_tag="VK031_02730"
     CDS             complement(2385..2837)
                     /locus_tag="VK031_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52859.1"
                     /translation="MSFWIKLGAITAIIITLLGLHTYYKRKAVKTAVNALKIEYKEEK
                     ALAEEKANRITKELLSSAMSAINKKDEEIKEINSALSYLSDRLRERPSREDSSRDTSS
                     TFSCTGAELSREDGEFLAREAARADRILKERDYYYERYEEARKALAAH"
     gene            complement(2816..3265)
                     /locus_tag="VK031_02735"
     CDS             complement(2816..3265)
                     /locus_tag="VK031_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HLS52860.1"
                     /translation="MMKRAIAASAIAISAIAGYEGLSLVAYKDPVGIPTICYGYTKDV
                     FMGMTKTVQECQYLLESEVEKFSKGVLDLYSGPITQGELDAYTSLAYNIGLDAFSRST
                     LLKKLKSGDRKGACNELRRWVYAGGKVLRGLEARRKAEERMCLSGLN"
BASE COUNT          904 a          727 c          544 g         1158 t
ORIGIN      
        1 aatgcctccc acttcaacca atcatcattg gggaacaaag cactgatata gttggaataa
       61 agattgtctc gtatctgaca aagcttagga agagttgttt tatttttcca tggtaatgaa
      121 gcattactgg tggtagaagt atctgtagca aatacatagt ttctcacctc tttccattct
      181 tctaatttag cttgcctctg agagttgaaa ttaaaccaag tttgggatat aaattcggcc
      241 tctctgtctc ttttaaaatg tacacctaat tgtaatggtt tactcataat taacctctaa
      301 aggccactcc tccaaatcta gagttaaaaa caactctgtt gtctctggct ccatccatac
      361 ttctcctatc tcttggtcta actgcaatag atactacagc ggctaatgcg tccttcatat
      421 cgtcgtgagg aggcctagac aataataact cttcttctaa caaaggggtt agtcctccct
      481 cgaaatgcca catcttcata tcttcgtact taggttctaa cgcagccgca atacgctctt
      541 ctttacttcc ttcggatcta ttaggtctgt attcttcgat aggaagacta agccctctat
      601 ctctcattct atctttaaga tctctaacta ttataacttg tgcagcagtt gtttctgctc
      661 ttaatttctt aaaatcccac ctagaatgta gttttacaat ccgatcataa tattctgaga
      721 tcttatctgt tttaaatcta tctatatcta gtacatagat attactctcg gggtctatcc
      781 ctattactac aatagctgaa tagtctgccc tttttcttaa agagaaagca aagtctatgg
      841 aagcgtatac gtttaatctt ttatctttaa aataccagtt tccagacttt ctttttataa
      901 atttaggggt gtagtattgg aagcaactag ggcttattct gttagatccg ctttcgttag
      961 ggttgttgta atattgggca aaaaattgag ttctatcttc atattctgcc tcaatcctag
     1021 acaacgtttg tctgtcaaac ccgaagaatt ttccatcaga agctctcaca gtcctgggcc
     1081 ataagaatac cccatctgtt tccactgtat gctctttaac ttcccataca ggaactctgt
     1141 ctacagtttc tccggattca tcgtatatat cgtaagcttg ttctttccat agagcataaa
     1201 catctgaagg gtggtatcta gttccacaag ccagggtgaa gcctcctgca ttacgaatgg
     1261 aggaaaactg agaactcttc ttcataacag agtctctacc ttcttgtgta taagcattct
     1321 ctggaactac taagtcgtct gccacaacta tatctgcatg ccatccagta gtgttagtag
     1381 tgaggcctgc tgtagctacg gttgggtccc taataccttc ttgtcttctt tttgcatgat
     1441 ctatagagaa tcgcctttgg ctccatcttt cccttttccc ttcctgaggg tttatatatt
     1501 ctggaaagta cctttgatac acatcactag ctaatatatt ttggatagag aataattgtg
     1561 tttctgcaag ctctgaagta gcagatacat ataagatagt tacttcagga tgcctagtaa
     1621 taatccaggc tacccaagtt gccacgcagt gcgatttgag atgtgctcta ggaagcataa
     1681 taagcttgtt agaggatagt ccttctcctt gcccaaataa agaatattct gaaatccatt
     1741 taaaaacttc tttatgtatt tccccatata catatcctgg gtttactaat ctagcaaaga
     1801 aatataaatc tgttaaggca gtttcccgta cctctttagc ctcttctggc atcctagaga
     1861 gcctatctgt tgcttttcta atccattcat ttctcatatc ttattttata gcgactaatc
     1921 gtctcaaatc gtcagcatat tcgtccttaa tattattctg gattttagcc tctctttcta
     1981 attcttcttt agaaggtctt cctgcttttc gtacttccca acccctatct gttaaaaacc
     2041 tagctgcccc ttgagaacct ctcctagcag ctttgaccat ctgactaatt ccttctgatc
     2101 tgagttttaa ttctaattca tatctccact cctcgatatg atcggtcatc aatttgttat
     2161 tacaaatacg caaccaatgt ctcatgttga ggaaatactt attagcaaat ctgtattcag
     2221 ttgggtcgtc ctcttccata tacagtttct taatagaagg ataaatctta ccattatatt
     2281 catgatcctg gtctttaaga gtgtatatgg caaaaggaga gtatgaaaac tctaagaaca
     2341 acgattgggt taaaggtctg ccagaagtat ctagcatttg gtctttagtg ggcagcaaga
     2401 gcttttcttg cttcttcgta acgctcataa taataatccc tttcttttaa tattctatct
     2461 gctctggcag cctcccttgc aagaaactct ccatcctctc tagaaagctc ggctcctgtg
     2521 caggagaagg tagaggaggt atctctggaa ctgtcttccc tacttggacg ctcccgcaac
     2581 ctatcagaaa gataggaaag agcagaatta atttctttaa tttcttcatc ttttttattt
     2641 atggcggaca tagccgaaga taataattcc ttagttattc tattagcctt ttcttctgct
     2701 agagccttct cctctttata ttcgatttta agggcgttta cagccgtttt aacagctttt
     2761 cttttataat aggtatgaag acctagaaga gttattataa tagctgtaat cgctcctaat
     2821 ttaatccaga aagacacatc ctctcctctg cctttcttct agcttctagt cctctaagaa
     2881 cttttcctcc tgcatacacc catcttctta actcattaca agctcctttt ctatccccag
     2941 attttaattt ctttaataaa gtgcttctgg aaaaagcatc tagacctata ttgtaagcta
     3001 aggaggtgta tgcatcaagc tctccttgtg tgatggggcc ggaatagaga tctaaaaccc
     3061 ctttagagaa tttctctact tcagattcta gaagatattg gcactcttgt acagttttag
     3121 tcatccccat aaatacgtcc ttagtgtatc cataacatat tgtaggaata cctaccggat
     3181 ctttataggc aactaagctg agaccttcgt atccggctat tgccgaaatt gcaatagcag
     3241 aagcagcaat agcccttttc atcattttct ttttcctgtt atggttttta taaatttagg
     3301 ggcaattaat attaggttta taaaaatata aac
//
LOCUS       DATGSI010000172         1257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_342145, whole genome shotgun sequence.
ACCESSION   DATGSI010000172 DATGSI010000000
VERSION     DATGSI010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342145"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..949
                     /gene="trxB"
                     /locus_tag="VK031_02740"
     CDS             14..949
                     /gene="trxB"
                     /locus_tag="VK031_02740"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HLS52861.1"
                     /translation="MIYDVVIIGGGPAGLSAGLYAARARLKTLIIEKEAYGGQIATTE
                     DVENYPGSITEPTGPELVERMIEQVEDFGAERKTDEITEVDFSGKLKLVKGKEEYKAK
                     TVIIATGAVPRPIGCPGEEEFTGRGVSYCATCDGPFFEGLEVFVVGGGDSAVEEAIFL
                     TRFAKKVTIIHRRDELRAAKSLQERAFKNPKIDFMWDTIVTEMRGEGMLGSIVVKNVK
                     TGEEKEIFPDENDGAFGVFVFVGNLPATEIFDGIVDMEQGYIVTDADMKTNVAGVFAA
                     GDNRVKSLRQVVTATADGAIAAVQAEKYIEENFEE"
     gene            960..>1257
                     /locus_tag="VK031_02745"
     CDS             960..>1257
                     /locus_tag="VK031_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLS52862.1"
                     /translation="MLKLDKDNFEKEVLQEKGYVLVDFWSPTCQPCKALMPHVEKLSE
                     EYGDKIKFTALDTSKARRVAIGQKVMGLPALVIYKNGEKVESLIGQAATEEAVEE"
BASE COUNT          443 a          169 c          343 g          302 t
ORIGIN      
        1 aggaggataa aagatgatat atgatgttgt aattataggt ggaggaccgg caggcctatc
       61 tgcaggttta tatgcggcta gagcccggtt aaaaacatta attattgaaa aggaggccta
      121 tggtgggcag attgccacca cagaagatgt ggagaattat ccaggttcta ttacagaacc
      181 gacagggcct gaattggttg aaagaatgat tgagcaagta gaggattttg gtgcagaaag
      241 aaaaacagat gagattacag aggtagattt tagtggaaaa ctaaaattag taaaggggaa
      301 ggaagaatat aaagcaaaaa cggtaattat tgcaacaggg gcagtgccta gaccaatagg
      361 ttgtccagga gaggaagaat ttacagggag aggagtttcc tactgtgcca cttgcgatgg
      421 accttttttt gaaggactgg aagtttttgt ggtaggaggc ggagactctg cagtagaaga
      481 agccatcttc ctaacaaggt ttgctaagaa ggtaaccatt atccatagaa gggatgagct
      541 aagggcagcc aaatctttac aagaaagagc ctttaaaaat cctaaaatag attttatgtg
      601 ggatacaata gttacagaaa tgaggggcga aggcatgcta ggctcaatag tggttaaaaa
      661 tgtaaagact ggggaggaga aagagatttt cccagatgaa aatgatggag cctttggagt
      721 atttgtgttt gtagggaatt tgccagccac agaaatattt gatggtatag tagatatgga
      781 gcaaggctat attgtaaccg atgcagatat gaaaactaat gtagcaggtg tctttgcagc
      841 tggagacaat agggtaaaat ccttaaggca ggttgttaca gccacagctg acggggctat
      901 agctgctgtt caagctgaga aatatataga ggaaaacttt gaagaataga ggtggtaaaa
      961 tgcttaaatt agataaggat aattttgaaa aggaagtttt acaggaaaaa ggatatgtgc
     1021 tggtagactt ttggagtcca acctgccaac cttgtaaggc cctgatgccg catgtagaaa
     1081 aactatcaga agaatatgga gacaagataa aatttacagc cttagataca tcaaaggcaa
     1141 gaagggtggc cattggacaa aaagttatgg gtttaccggc cctagtaata tataaaaacg
     1201 gagaaaaggt agaatcctta atagggcagg cagctacaga ggaagcagtg gaggaaa
//
LOCUS       DATGSI010000173         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_13220, whole genome shotgun sequence.
ACCESSION   DATGSI010000173 DATGSI010000000
VERSION     DATGSI010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13220"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..>1023)
                     /gene="metK"
                     /locus_tag="VK031_02750"
     CDS             complement(224..>1023)
                     /gene="metK"
                     /locus_tag="VK031_02750"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HLS52863.1"
                     /translation="DQGMVFGYACDETEEYMPLTLSLAHKLSRRLSVVRKEGILDYLR
                     PDGKSQVTVEYIDGVPKRVDAIVVSSQHDSEVTTEQLRTDIKKYVIDEIIPAELMDEK
                     TRIFINPTGRFEIGGPMGDAGLTGRKIIVDTYGGFGRHGGGAFSGKDATKVDRSAAYM
                     ARYIAKNLVASGLANKAEVGLSYAIGVAKPVAIYVDTFGTGKISDEEIAKIVEEKFDT
                     RPAAIIDKLQLRKPVYKQVAAYGHFGRNDLDLSWEKLDMVDELKKYL"
BASE COUNT          296 a          203 c          112 g          412 t
ORIGIN      
        1 ttataactac acctttactt cttgtatata cgagtacaga agctgttcct aaatctatag
       61 caatatttct tctaaattta gccatcaaaa tatacttcct ctcactgtta tttactacaa
      121 attatcaact tataatatat cataagtatt taaatttata aatagtactt ttttataaaa
      181 aaataaagcc gaggtaattt cctcggcttt taatttcttt tatttaaaga tattttttta
      241 attcatctac catatctaat ttttcccaag ataaatctag atcatttctt ccaaaatgtc
      301 cataagctgc cacttgttta tatactggtt ttcttaattg taatttatct ataattgctg
      361 ctggtcttgt atcaaatttt tcttctacta tttttgctat ttcttcatct gatattttac
      421 ctgtgccaaa agtatctaca tatatagcta ctggtttagc aacacctatt gcataagata
      481 aacctacttc agctttattt gcaagtcctg aagcaactaa gtttttagct atatatctag
      541 ccatataagc agcagatcta tctactttag ttgcatcttt acctgaaaat gctccaccgc
      601 catgtctacc aaatccacca taagtgtcta ctataatttt tcttcctgta agacctgcat
      661 ctcccatagg tccacctatc tcaaatctac ctgttgggtt tataaagatt ctagtttttt
      721 catccattaa ttctgctgga attatttcat ctattacata tttctttata tcagttctca
      781 attgttctgt agtaacttcg ctatcgtgtt gagatgatac tactattgcg tctactcttt
      841 ttggaacacc atctatatat tctactgtta cttggctttt accgtctggt cttagatagt
      901 ctaatatacc ttcttttctg actacagaaa gtcttcttga taatttatgt gcaagactta
      961 aagttaatgg catatattct tcagtttcat cacaagcata tccaaatacc ataccttggt
     1021 ctc
//
LOCUS       DATGSI010000174         2998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_247284, whole genome shotgun sequence.
ACCESSION   DATGSI010000174 DATGSI010000000
VERSION     DATGSI010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247284"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..327)
                     /locus_tag="VK031_02755"
     CDS             complement(43..327)
                     /locus_tag="VK031_02755"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HLS52864.1"
                     /translation="MAKILVACGAGIATSTMASNKLKEGLKERGKLQGVTIDQCKVAE
                     VASKAQGYDLIVSTTQVPSRVEKPVVNGIPFLTGVGLDDAIEEVIEKLDL"
     gene            complement(446..1339)
                     /locus_tag="VK031_02760"
     CDS             complement(446..1339)
                     /locus_tag="VK031_02760"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HLS52865.1"
                     /translation="MRVAKRDPVYNVHSPLNKPNLSVKQGETFKVETELNGGPWLKSI
                     EDRWDPSKSRGPNLTTVVEVLNSKKGEVLAVEILDIEVDNIGYTGFAGWRLPLSQEIL
                     PNDWDVVTKTVAIEDGYVLWDEDLKLPIDPMIGTIGVAPAEGEISNAIAYRHGGNFDV
                     QEIRKGTTLYLPVNVDGALLHIGDVHAIMGDGEINNGGGIECRGEVTLRVKSIEKPAS
                     MDWFRAESHEHIMTIACEKDVKKSFTSATRELILWMRDDYGFSEKEAYLLLGQVMEAR
                     CTVFVNDTYSYICKINKKYLT"
     gene            1940..>2998
                     /locus_tag="VK031_02765"
     CDS             1940..>2998
                     /locus_tag="VK031_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLS52866.1"
                     /translation="MLKSTGWEAVVAALKEEGIKYVFGMPSSTQDLYDALYDEPTIEA
                     IQVRHEVAGGFMAMAYSLLTGEPAICFASQGPGITNLTSAMLESLATCAPVIAICPGT
                     DGTKDGKGAFQETDQMGIMKPITKWAYRVEQAEQIPWAISRAFYLATNGKPGPIYIEI
                     PTNIGRGEYQIPKYVSPPRRLRYAADSKSIKEAIDLLLEAERPLIISGGGARHSRAHK
                     EVRRLSEILGAPIMTTPSGRGIVTEDYPLYFGQVGLYRNEIGIDINQETDLIITIGSR
                     NEEFQTGGWRFLPKNCKLIQIDIDAFEINRNWIPDVPILGDAKLVLKDLINKLDGEPR
                     DKWAERAKKYADIKLAFDR"
BASE COUNT          940 a          600 c          486 g          972 t
ORIGIN      
        1 tcaaatactt ctagaattac catttataag ccccctcttc tcttataagt caagtttttc
       61 aattacttct tcaatagcat catctaaacc tactcctgtt aaaaaaggaa tgccatttac
      121 aacaggcttt tccactcttg aaggtacctg ggttgtagat acaatcagat catagccttg
      181 agcctttgaa gcaacctcag ccaccttaca ctgatcaatc gtaactcctt gtaacttgcc
      241 tctttccttt aaaccttcct ttagcttgtt tgatgccata gtggaagtag caattcctgc
      301 tccacaagcc actaatattt ttgccatatt ttcacctccc tattcagaat atacacttat
      361 gacaccctta atcaaggtca atgcctagat tgtttttata ttagaatccc tgtaaacagg
      421 gtaattccct atgttagaaa aactattagg taagatactt tttgttaatc ttgcaaatat
      481 aagagtaagt atcattgaca aatacagtac agcgggcctc cattacctga cctaacaaca
      541 aataggcttc cttttcacta aatccataat catcacgcat ccaaaggatt aattctctag
      601 tagcagaagt aaaggatttt tttacatcct tttcacaggc aatagtcatt atatgttcat
      661 gggactcagc ccgaaaccaa tccatgctgg ccggtttttc tatactcttt acccttaagg
      721 ttacctctcc cctacactca atacctccac cattgtttat ttcgccatct cccataatag
      781 catgaacatc acctatatgt aataaggctc catccacatt tactggcaga taaagggtgg
      841 ttccttttcg gatttcttga acatcaaaat tgccaccatg tctataggca atagcattag
      901 atatttcccc ttcagcagga gccacaccta tggttcctat cataggatca ataggtagtt
      961 ttaaatcctc atcccacagc acatatccat cttctatggc cactgttttt gtaaccacat
     1021 cccaatcatt aggtaggatt tcctgagaca ggggcaatct ccaacctgca aaaccagtat
     1081 atcctatatt atctacctct atgtcaagta tttcaacagc taaaacttct ccctttttag
     1141 agtttaaaac ttctacaaca gtagttaagt taggacccct gcttttactt ggatcccacc
     1201 tatcttctat acttttaagc cagggaccac cattaagttc ggtctctacc ttgaatgtct
     1261 ccccttgttt tactgataag tttggtttat ttaaagggct atgcacatta taaacaggat
     1321 cccttttagc aactctcatt tatatatcac cttatcctat attttaagat ttatacctgt
     1381 aaggttaata ttattagaac ttttatttta taataaaact acttcatttt gcaatatttt
     1441 tcgtatatta gtatccttgg caagtgtttt cctagttata agaaaaaaat agctctttta
     1501 cacttcaacc ttttcatttc tctttagata agctatttta aaaaatccat catggaaaat
     1561 ttctgtaatt gtatattatt aagtatttgc aagatttaca agtttaatct gcttacaatt
     1621 tgtttaatta taccataatg ttaagcagta tcaatattta actaatgata atttttgaat
     1681 tattttttac aaatggtata atcatattat agcacatttt ttataaaaat gctgggaaaa
     1741 atatattttt ttaaaattat tatatataaa ttgtccctta gactaaattt accccctggc
     1801 tacatatata aactattaaa gaaaatttat ataatattaa tatattaggg taaaacgtta
     1861 ccagatcagg ttttaattgt ggtctttaaa cacttcattt atgtgtttga aatatactta
     1921 tgaaaaggag ctgattaaaa tgttaaaatc tactggttgg gaagctgttg tagctgcctt
     1981 gaaagaggaa ggtataaaat acgtttttgg catgcctagc agcacccaag acctatacga
     2041 tgccctttat gatgaaccaa caatagaggc catccaagtc agacatgagg tggcaggtgg
     2101 ctttatggcc atggcctact ctcttttaac tggagaacca gcaatctgct ttgctagcca
     2161 gggtccggga attacaaatt taacatcagc tatgttagag tctttagcca cctgtgcacc
     2221 tgttattgcc atttgtccag gcaccgatgg gaccaaggat ggtaaaggtg cctttcaaga
     2281 aacagaccaa atgggaatca tgaagcctat taccaaatgg gcctataggg ttgagcaggc
     2341 ggaacaaatt ccttgggcca ttagccgagc cttttattta gccaccaatg gtaaaccagg
     2401 ccctatttat attgaaatac ctacaaatat tggaagaggt gagtaccaaa tccctaaata
     2461 tgtatctcct ccacgccgtc taaggtatgc agcagattca aaaagtatta aagaagccat
     2521 agatctttta ctggaggcag aaagaccttt aattatatct ggtggaggtg ctagacattc
     2581 tcgggcccat aaagaggtta gaaggctgtc agaaattttg ggggccccta ttatgaccac
     2641 tccttcaggc aggggaatag ttacagagga ctatccctta tacttcggtc aagtaggtct
     2701 atacagaaat gaaatcggca ttgatataaa tcaagaaact gaccttataa tcaccatagg
     2761 ctctcgtaat gaagaatttc agactggagg ttggagattt cttccaaaga attgtaaatt
     2821 aatacaaata gatatagatg cctttgaaat taacagaaat tggatcccag atgttcctat
     2881 cttaggagat gctaagcttg ttttgaagga tttaatcaat aaattagatg gtgagcctag
     2941 ggacaagtgg gcagaaaggg caaaaaaata cgcagatata aaattagcct ttgatagg
//
LOCUS       DATGSI010000175         1989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_392246, whole genome shotgun sequence.
ACCESSION   DATGSI010000175 DATGSI010000000
VERSION     DATGSI010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392246"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VK031_02770"
     CDS             <1..183
                     /locus_tag="VK031_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52867.1"
                     /translation="LGDITTIIIAQRISSIQEADTIIVMHEGEIESMGDHETLLEISP
                     IYKEIYESQEKGVIKE"
     gene            180..>1989
                     /locus_tag="VK031_02775"
     CDS             180..>1989
                     /locus_tag="VK031_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS52868.1"
                     /translation="MTSNNRRKQLRPENPMRTIRRLFGYFKHNKLLLFGGIFFIILGA
                     LGQISVNAMLSPVIDTLVENKPLSLFIRYLIIMGIIVIFLALGEYLGNLFMVRLAQRT
                     VHKIREDMFSNMETLPITFFDQHSHGELMSTFTNDVDMLNQALEQNVSQIIRTVITFF
                     GTLFMMLYLSPSLTLVVIGMLLIMFFTIKVIGSKSSENFRIQQYALADMNGYIEEIMS
                     GQKVVKVFNYEDRAIEEFVGANEELRQASTLAATYGIMLMPVMGSLSYVLYAFVAIIG
                     AFLTIFGSLTIGNIASFLQYTRTISRPITQVSNQLNTLFAALAGAERIFAILDEEPEV
                     DDGDVELVMCKSRRDCYWNVPQEDGTHKRIPLRGHIKFENVDFGYAEDKLVLKDINLY
                     AKPGQKIAFVGSTGAGKTTITNLINRFYEINEGRILFDGIDIRRIKKFDLRSTMSIVL
                     QDVHLFKGTVADNIRYGRLDATDEEVVEAAKLANAHYFISHLPKGYDTMLTTDGQNLS
                     QGERQLLSIARAAIADPTILILDEATSSVDTRTEKLIEKGMDKLMEGRTTFVIAHRLS
                     TVRHSDAIMVLEQGEIIERGSHQELLEQKGRYYQLST"
BASE COUNT          647 a          339 c          416 g          587 t
ORIGIN      
        1 ctgggagata taaccaccat aatcattgca caaagaattt cgtctatcca agaggcggat
       61 actataatag ttatgcatga aggtgaaatt gaatctatgg gtgatcatga aactttatta
      121 gaaatatcac caatttataa ggaaatatat gagtcccagg aaaaaggggt gatcaaggaa
      181 tgaccagcaa caataggaga aaacaactaa gaccagaaaa tccaatgagg actataagga
      241 gactgtttgg ctactttaaa cataataaac tcttgttatt tggcggaata tttttcataa
      301 tccttggggc cttaggacaa atatctgtaa atgctatgtt aagtcctgta atagatactc
      361 tagtggaaaa taaaccttta tccctcttca ttagatacct gattataatg gggataatag
      421 ttatattcct agcattaggt gaatatcttg gcaacctatt tatggtaagg ctagcccaaa
      481 gaactgttca caaaatccgg gaagatatgt tttctaatat ggaaaccttg cctattacct
      541 tctttgacca gcattcccat ggagaattaa tgtctacatt tacaaacgat gtggatatgt
      601 taaaccaggc tttagagcaa aatgtttcac aaatcattag aactgttata accttctttg
      661 gaaccctgtt tatgatgcta tatttaagtc cctcactcac tctggttgtg ataggtatgc
      721 tgttaatcat gttttttacc ataaaggtaa ttggttctaa gtcatcggag aactttagaa
      781 tccagcaata tgccttggca gatatgaacg gttatattga ggagattatg tctggccaaa
      841 aggtagtaaa ggtatttaac tatgaggata gggccataga agagtttgta ggagccaatg
      901 aggagttaag gcaagccagc accttggctg ccacctatgg gattatgctt atgcctgtaa
      961 tgggtagcct gtcctatgtt ttatatgctt ttgtggccat aattggtgca tttttaacca
     1021 tattcggttc cttaaccatt ggaaacatag cctccttttt acaatataca aggaccatat
     1081 ctcgaccaat aactcaggtt tccaaccagc taaatacctt gtttgcagcc ctggctggtg
     1141 ctgaaaggat ctttgccatc ctagatgagg agcctgaagt tgatgatggt gatgtggaac
     1201 ttgttatgtg taaaagtaga agggattgtt attggaatgt gcctcaagaa gatggaaccc
     1261 ataagagaat ccctcttagg ggccatatta aatttgaaaa tgttgacttc ggctatgctg
     1321 aggataaact agtattaaaa gatataaacc tttatgcaaa acctggtcaa aaaattgctt
     1381 ttgtaggttc cactggagca ggaaagacta ctattaccaa cctaattaat aggttctatg
     1441 aaataaatga aggtagaatc ctgtttgatg gcattgatat tagaaggatt aaaaagtttg
     1501 atctaagaag tactatgagt atagttctac aggatgtaca tctatttaag ggtacagttg
     1561 cagataacat tagatatggt agactagatg ccactgatga agaggttgta gaggctgcca
     1621 aattagccaa tgcccactac tttatcagcc atctacctaa gggctacgat accatgctta
     1681 caactgatgg acaaaactta tctcaaggtg aaagacaatt gctttctata gctagggcgg
     1741 ccatagctga cccaactatt ttaatcctgg atgaagctac ttcttctgtt gatacaagaa
     1801 cagaaaagct aatagaaaag ggtatggata aacttatgga aggcaggacc acctttgtaa
     1861 tagcccaccg cctatctact gtaaggcatt ctgatgctat tatggtattg gagcagggag
     1921 aaattattga aagaggtagt caccaagaac tactggagca aaaaggccgt tattaccagt
     1981 taagcactg
//
LOCUS       DATGSI010000176         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_294613, whole genome shotgun sequence.
ACCESSION   DATGSI010000176 DATGSI010000000
VERSION     DATGSI010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294613"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VK031_02780"
     CDS             <1..421
                     /locus_tag="VK031_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLS52869.1"
                     /translation="ALLFFGLGKAPGVIATTIYAIVPIIRLTNHGIRQIDEEVVEASL
                     AFGSTWIQSLIKVQIPQALPTIMTGVNQTLMMAISMVVTTSMIGVRGLGMEVIDGVNK
                     IEIARGLLSGISVVIMAVILDRLTQGLVQRSEVRSDG"
     gene            414..1595
                     /locus_tag="VK031_02785"
     CDS             414..1595
                     /locus_tag="VK031_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HLS52870.1"
                     /translation="MDNNDTILRVENLCKLYGPEKSRAIKMKEAGASKDEIYEKTKVT
                     VALWDASFEVKEGETFVVIGLSGSGKSTLVRCFNRLNKPTRGKVFYKGQDIGKFNKEK
                     LNDYRRNKISMVFQQFGLMSHRSVLGNVEYGLEIKGMSKDERQRIAKEMISMVGLEGQ
                     EDEPIFSLSGGMKQRVGIARALANDPDILLMDEPFSALDPLVRKDMQFELLTIQKKLN
                     KTIIFITHDINEAFKIGDRVAIMRDGEVIQIATPEEMSENPADDYVEQFIDSADKTQV
                     IHVKNVMITPNSIVRLRDSLNFAIKTMRSNGVSSAYVVGEKMKLQGVVTIDDALAARK
                     ENLSLADILIRDINTTSPDALLSDIMSMATETPFPIAVVDEDNALKGIVSKVHVLSSM
                     L"
     gene            complement(1653..>3247)
                     /locus_tag="VK031_02790"
     CDS             complement(1653..>3247)
                     /locus_tag="VK031_02790"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HLS52871.1"
                     /translation="AKYLARTYSYGGGCGLTLSKLRPKGAKVNNAANTTTGAVSFMDL
                     YSLVTGLIGMRGRRGALMLNLDVNHPDIVDFINVKNDLDKVTYANISVNIYDDFMEAV
                     ENDEDYELYFKVEATGEEIRKVVKAKELFRLIARNNWNMAEPGILYQDRINSWHLMSE
                     DETFEFAGVNPCAEETLPAFGSCNLSSINLSEFVKNPFTEYALFEFEKFGEMVRSGVI
                     YLNEVLDENMNLHPLPQQREMSKELRQIGLGIMGVADMFIKLGIRYGSPESISLIHQI
                     GRTMINEALRQSALLAKEEGPFPRYRKEAVLKSPFLQSNADDDVLELIEKYGLRNSQL
                     LTIPPTGSISTLVGCSNGLEPIFQVSYTRKSESLFEEDTYYKVFTPIVKEYMDKNNIT
                     REEDLPEFIVTTSNLDYRERIEVQATWQQYIDASISSTVNVPNEFTVEEVEDLYMYAW
                     KMGLKGVTIYRDGCARSGILITEKSKSRVDRINELQEELNTLAAEELMENPDVCPMCG
                     GELIHSGGCDECQDCGYSPCSI"
BASE COUNT         1023 a          604 c          595 g         1025 t
ORIGIN      
        1 tgccctttta ttctttggac taggtaaggc accaggggtt atagccacta ccatttatgc
       61 catagttccc ataatcaggt taactaatca cggaatcagg caaattgatg aagaagtggt
      121 agaagcctcc ttggccttcg gttccacatg gatccaatcc ttgattaagg ttcagattcc
      181 ccaggcatta cctactatta tgaccggtgt aaatcaaacc ttaatgatgg caatctccat
      241 ggtagtgacc acctccatga ttggagttag gggattaggt atggaagtta ttgatggagt
      301 aaataaaata gaaatagctc gtggattatt gtctggtatt tctgtagtaa ttatggcagt
      361 tatattagac agattaaccc aggggttagt ccaacgtagc gaggtgagat cagatggata
      421 ataatgatac tatattaaga gtagaaaatt tgtgtaaact atatggccca gaaaaatcaa
      481 gggcaataaa gatgaaggaa gctggagcta gcaaagatga gatctatgag aaaactaagg
      541 ttacagtggc cctatgggat gctagttttg aggttaagga aggagaaacc ttcgttgtta
      601 taggtttatc tggctcaggt aaatcaacct tagttagatg ttttaaccgc cttaataagc
      661 ctaccagggg taaagttttt tacaagggcc aggacatagg taaatttaat aaggaaaaat
      721 taaatgatta tagaagaaat aaaatttcca tggtctttca acaatttggg ctcatgtccc
      781 acagaagtgt attaggtaat gtggaatatg gtttagaaat taagggaatg tccaaagatg
      841 aaagacaaag aatagcaaag gaaatgatat ctatggtagg tttggaaggg caagaagatg
      901 aacctatctt tagtctttct ggaggtatga agcaaagggt tgggattgct agggccttgg
      961 ccaatgatcc tgacattctt ctaatggatg agcctttctc agccctggat ccattggtta
     1021 gaaaagatat gcaatttgaa ctactaacta tacaaaagaa gttaaataaa actattatat
     1081 ttattacaca tgatattaat gaagccttta agattggaga cagggtagcc attatgagag
     1141 atggtgaggt tatacaaata gctacaccag aagaaatgag tgaaaatcct gccgatgatt
     1201 atgtagaaca atttatagac agtgcagata agacccaagt aatccatgtt aaaaatgtaa
     1261 tgattacccc taatagtata gttaggctaa gggacagctt gaattttgca atcaagacca
     1321 tgagaagcaa tggagtgtcc agtgcctatg ttgtagggga aaaaatgaaa ctccaagggg
     1381 ttgtaactat agatgatgcc ctggctgcaa ggaaggagaa tttaagcctg gcagatattt
     1441 taataagaga tataaacact acaagcccag atgctttact atcagatata atgagtatgg
     1501 caactgaaac tcctttccca atagctgtag tagatgagga taatgcttta aagggtattg
     1561 tatctaaggt tcatgtacta tcgtctatgt tatagtaaat ttaaagggac atgtaaagtt
     1621 ttacatgtcc ctttttatat ccatcttatc tattaaattg agcaagggct atatccacaa
     1681 tcctgacact catcacagcc acctgaatgg attaattctc ctccacacat agggcaaaca
     1741 tcaggatttt ccattaattc ttctgcagct aaggtattta actcttcctg cagttcatta
     1801 atcctatcta ccctgctttt agatttctct gtaattaaga ttccacttct ggcacaacca
     1861 tctctgtata tggttacacc tttcaggccc attttccatg catacatata tagatcttct
     1921 acttcttcta cagtaaactc attaggtaca ttcacagtag atgaaatact tgcatctata
     1981 tattgttgcc aggttgcctg tacttctatc ctttccctat aatctagatt tgaggtggtt
     2041 actataaatt caggcaggtc ctcttctcta gttatattgt tcttgtccat atattccttt
     2101 actataggtg taaatacctt atagtaggta tcctcttcaa ataaggattc agattttcta
     2161 gtataggaaa cttggaatat tggctccaaa ccattactgc aacctaccaa ggttgatata
     2221 ctaccagttg gtggaatagt taacagctgg ctatttctta gtccatattt ttctattaat
     2281 tctaatacat catcatcggc atttgactgt aaaaatggtg acttaagcac agcctctttc
     2341 ctatatcgtg ggaaaggtcc ttcctccttt gccagtagag cagattgtct tagggcttca
     2401 tttatcatgg tcctacctat ttggtgaatt aggcttatag actcagggct tccatatcta
     2461 attcccagct taataaacat atctgcaact cccataatcc ctagccctat ttgtcttagc
     2521 tctttagaca tctccctttg ttgtggtaga gggtgcaaat tcatattttc atctaaaact
     2581 tcatttaaat atataactcc acttcttacc atttcaccaa acttttcaaa ttcaaacaag
     2641 gcatactcag tgaaaggatt cttaacaaat tcacttaaat taatggaaga taaattacaa
     2701 ctaccaaagg ctggtaaggt ctcctctgca caaggattta ctcctgcaaa ttcaaaggtt
     2761 tcatcttcac tcatcaagtg ccaggaattt atcctatcct gataaagaat ccctggttct
     2821 gccatattcc agttgtttct agctattaat ctaaataatt ccttagcctt tacaaccttt
     2881 cttatttctt ctccagttgc ttctacctta aagtaaagct cataatcttc atcattttca
     2941 acagcttcca taaaatcatc atatatatta acggaaatat tagcataggt taccttatca
     3001 agatcatttt taacattgat aaagtctaca atatctggat ggtttacatc aagatttaac
     3061 attaaggccc ctcttcggcc tctcatacca attaatccag tgactaagga atatagatcc
     3121 ataaaggata cagcacctgt agtagtatta gctgcattgt ttaccttggc tccctttggc
     3181 cttaatttag ataaagtcaa tccacaccca ccaccatagg aataagtcct ggctaagtac
     3241 ttggctg
//
LOCUS       DATGSI010000177         1076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_59799, whole genome shotgun sequence.
ACCESSION   DATGSI010000177 DATGSI010000000
VERSION     DATGSI010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59799"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VK031_02795"
     CDS             complement(<1..792)
                     /locus_tag="VK031_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLS52872.1"
                     /translation="MKNPNGYGSVYKLSGNRRRPWIARVTTGWSDEGKQQYYTVGYFR
                     TRREGMEALAKYHKNPIAERGDITLKELYKEWSKGRYPKLAQKTVESYEVAWNHLSTL
                     KDEKLKDIKTSHIQKIINDMNKKGLSRSSCHKVKVLAGLIYKHALADDIVDRNYAEMV
                     ELPPKQSRKKEIFTDLEIKAIEELAKIDPWANTILILIYTGMRVGELLSLTKFNISID
                     DMVIVGGIKTDAGKDRIIPIHSKIQEHIKYWYNTNSDYLINREGKK"
     gene            complement(805..>1076)
                     /locus_tag="VK031_02800"
     CDS             complement(805..>1076)
                     /locus_tag="VK031_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HLS52873.1"
                     /translation="CLKVKGDSMIGARINDGDIVFIREQPCVENGEIAAVMIDNEVTL
                     KRFYKNGGLVMLRSENPKYQPLMYTKEDFKQVRILGKAIFFQSKL"
BASE COUNT          284 a          226 c          131 g          435 t
ORIGIN      
        1 ttttttacct tctctattta ttaaataatc actatttgta ttgtaccaat attttatatg
       61 ttcttgtatc ttagagtgta tgggaattat tctatctttg cctgcatctg tttttatacc
      121 gcctactatc accatatcat ctatacttat attaaattta gttagactca ataactcccc
      181 taccctcatt ccggtgtaga ttagaattaa gattgtatta gcccaaggat ctatcttagc
      241 taattcctct atagccttaa tttctaagtc tgtaaagatt tctttcttcc tagattgttt
      301 aggtggtagt tctaccattt cagcataatt cctatctaca atatcatctg ctaaagcatg
      361 tttatagatt aatcctgcaa gaactttaac cttatgacat gatgatctac ttagtccttt
      421 tttattcata tcgtttatta ttttttgtat gtgtgaagtt tttatatctt taagtttttc
      481 atcttttaat gtagatagat ggttccaagc cacctcatag ctttctacag ttttttgggc
      541 cagctttggg taccttccct tggaccattc tttatataat tccttaagag ttatatctcc
      601 cctttcagca ataggatttt tatggtactt agctagagcc tccattccct ctctacgtgt
      661 tctaaaatat cccacagtat aatactgttg cttgccttcg tcactccagc ctgtggtcac
      721 tctggctatc cagggacgtc ttctattgcc agatagcttg tatacacttc cataaccatt
      781 aggatttttc attttatcac cttcctacaa cttgctttga aaaaagatag cctttcctaa
      841 tattctcact tgcttaaaat cttctttagt atacattaag ggttggtatt tagggttttc
      901 tgatcttagc attaccaaac caccattttt ataaaatctt tttagagtaa cttcattatc
      961 aatcattacc gctgcaatct caccattttc tacacaaggt tgttctctaa taaatactat
     1021 atctccatca tttattcttg cacctatcat agaatcaccc ttaactttca aacaaa
//
LOCUS       DATGSI010000178         1185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_59800, whole genome shotgun sequence.
ACCESSION   DATGSI010000178 DATGSI010000000
VERSION     DATGSI010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59800"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VK031_02805"
     CDS             <1..422
                     /locus_tag="VK031_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52874.1"
                     /translation="YHITTEGVKRKNFLMITYFQELIDVYFLVGQSMEFFFRDLENRG
                     IEKVLLYGAGKVAETILMVISGRTNKPLKVLAIIDDDPDLLGKDIQGYKIISKNQIPN
                     YPHDSIIITSYTYEDTIMKKLTDIKYPNEKIERLFTI"
     gene            440..>1185
                     /locus_tag="VK031_02810"
     CDS             440..>1185
                     /locus_tag="VK031_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLS52875.1"
                     /translation="MPTKAKGKNSRIYLSSPHMSDEGYELKYIQEAFDSNWIAPLGEN
                     VNMFEKELAEYVGSSNAAALSSGTAAIHLALKAAGVGKGDIVFCQDLTFAASCNPIIY
                     ENAIPVFIDSDYESWNMDPDLLEEAFEKYPDVKAVIVVHLYGLSANMDRIVELCQKHN
                     VTLIEDAAESLGTYYKGKHTGTFGQYGIFSFNGNKIITTSGGGMLVSEDAERIEKTRF
                     WATQARDKARHYQHSEIGYNYRMSNICAGIG"
BASE COUNT          442 a          183 c          246 g          314 t
ORIGIN      
        1 cctatcatat tactacagaa ggggtaaaga gaaaaaattt tttgatgatt acatacttcc
       61 aagaactaat agatgtatat tttttagttg gacaaagtat ggaattcttt tttagggatt
      121 tagaaaacag gggaatagag aaagtattat tgtatggagc agggaaagta gcagaaacca
      181 ttttaatggt aataagtggc aggacaaata aacctttaaa ggtattagca atcatagacg
      241 atgatcctga tttattaggt aaggacatcc aaggttataa aatcatttct aaaaatcaaa
      301 tacctaatta cccgcatgac agtattatta tcacatcata cacatacgaa gacaccatca
      361 tgaaaaaact tactgacata aaatacccca atgaaaaaat agaaagatta tttacaatat
      421 agtgtaggga ggttcaaata tgccaacaaa agctaagggc aaaaatagca ggatctatct
      481 ttcatcgcca catatgagtg atgaaggcta tgaattaaaa tacatacagg aggcttttga
      541 cagcaattgg atagcacctc taggggaaaa tgtaaatatg tttgaaaaag agctggctga
      601 gtatgtaggt agctcaaatg ctgcagccct atcatcaggg acagctgcca tacatctagc
      661 cctaaaggca gcaggagtag gcaagggaga tatagtcttt tgccaagact taacctttgc
      721 agcctcttgt aatccaatta tatatgaaaa tgcaattcca gtatttatag acagtgatta
      781 tgaaagctgg aatatggatc cagatttact agaagaggcc tttgaaaaat atcctgacgt
      841 aaaggcagtt atagtggtcc acctatatgg cctatctgcc aatatggata ggatagttga
      901 actatgccaa aaacataatg taactttaat agaagatgca gcagaaagtc taggtacata
      961 ctataaaggc aagcatactg gaacttttgg ccaatatggg atcttttcct ttaatggaaa
     1021 taaaataatc accacatcag gtggaggtat gctggtatca gaggatgcag aaagaattga
     1081 aaagaccagg ttttgggcaa cccaggcaag agataaggcc agacactacc aacatagtga
     1141 aatcggctat aattatagga tgagtaatat ttgtgcaggg attgg
//
LOCUS       DATGSI010000179         1924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_59817, whole genome shotgun sequence.
ACCESSION   DATGSI010000179 DATGSI010000000
VERSION     DATGSI010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59817"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VK031_02815"
     CDS             complement(<1..861)
                     /locus_tag="VK031_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HLS52876.1"
                     /translation="MPSFMKDIFNFVNKYIEIAIAFTIVAVIGIIIIPMPSAVLDFLL
                     VINISLGVIILLLTLFTRHVLEFSTFPTLLLITTMFRLALNISSTRLILSYGDAGKVI
                     DSFANFVTGNNYIVGGIIFVIIVIVQMVVVTNGASRVSEVAARFTLDAMPGKQMAIDA
                     DLNTGIITEEEAKKRRQDLQREADFYGAMDGASKFVKGDAIAGIIITIVNLLGGILLF
                     SVQGGLGVMEALDKFGKLTIGDGLVSQVPALLISIASGILVTRSEDEHGFGETVTEEL
                     FGFYRVIMVGA"
     gene            complement(882..>1924)
                     /gene="flhB"
                     /locus_tag="VK031_02820"
     CDS             complement(882..>1924)
                     /gene="flhB"
                     /locus_tag="VK031_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HLS52877.1"
                     /translation="EATPKRLREAREKGQVAKSADLNAAASFFIFTILAGLLSQYIYV
                     NGLTYLKNSLDTNLSLDISKNLSRLLLDHLIMFGLFLLPFLIIVIVLGVAINLAQIGF
                     IFTMEPLKPDLKRLNPIEGFKNIFSKKAFFTLIKNILKLVLIIYLTFKSLKKTISAVV
                     NTSRLGSAKLFPFFVDFAKDIGMTIAVVMLLLGIVDFIMERREYKKNLRMSVREVRDE
                     LKEMEGSPEIKSARQQRQRELAMTRMMANIESGTVVITNPTHIAVVLRYDSKIDKAPI
                     IIGKGVDYLAEKIKERAKEHKIPILEDKALARTIYDQVDIGEYIPIELYQAIAEILAL
                     VYQMEERNKGKI"
BASE COUNT          576 a          420 c          303 g          625 t
ORIGIN      
        1 agcccctacc ataatcaccc tgtaaaaacc aaataactct tctgttacag tttcaccaaa
       61 gccatgctca tcttctgatc tggtaaccaa aattcctgag gcaatagata ttagaagggc
      121 aggcacctgg ctgactaaac catcacctat ggttagtttc ccaaacttat ctaaggcctc
      181 cataaccccc aatccacctt ggactgaaaa gagcaagata cctcctagta aatttactat
      241 ggtgatgata ataccagcaa tggcatcccc cttaacaaac ttactggctc cgtccatggc
      301 tccgtaaaag tcggcctccc tttgtaaatc ctgtcgcctt tttttggcct cttcttcagt
      361 aattattcct gtatttaaat ctgcatcaat ggccatctgc tttcctggca tagcatctag
      421 ggtaaatctg gccgccactt cagaaacccg gctggcacca ttggttacta caaccatctg
      481 cactattact ataattacga aaataattcc acctactata taattattgc cagttacaaa
      541 gttggcaaag gaatctatta ccttacctgc atctccatag cttaaaatca gcctagttga
      601 agaaatattt aaggccagcc taaacatggt ggtaattaag agtagggtgg gaaaggtgga
      661 aaattccaat acatgcctag taaataaggt taaaagcagt attataacac ctaggcttat
      721 atttattacc agcaaaaagt ctaagacagc actgggcata ggtattatga ttataccgat
      781 tactgccaca atggtaaaag ctatggctat ttctatgtat ttattaacaa agttgaaaat
      841 atctttcata aagctaggca tcttcatact ccttcttgtc cttaaatttt gcccttattt
      901 ctttcttcca tctgatatac aagggctaat atttctgcaa tagcctggta taactctata
      961 ggaatatatt ctccaatatc tacctgatcg tatatggttc tggccaaggc cttgtcctcc
     1021 aaaataggga ttttgtgttc cttagccctt tctttaattt tttctgccaa ataatctacg
     1081 cccttgccta tgattattgg ggccttatca attttactat cgtatcttaa gactacagct
     1141 atgtgggttg gattggtgat aactactgtt ccactttcta tattggccat catcctagtc
     1201 atggccaatt ccctttgtct ttgctgcctg gctgatttga tttctggact accttccatc
     1261 tccttcagct cgtctcttac ttccctaact gacattctta aattcttttt atattctctt
     1321 ctttccatta taaaatctac tatacctaaa agtagcataa ccactgcaat agtcatacct
     1381 atatccttgg caaagtctac aaaaaaggga aacaatttgg cactacctaa cctgctagta
     1441 tttactactg cacttatggt ctttttcagg ctcttaaaag ttagatagat aattaggact
     1501 aactttaaaa tattttttat caaggtaaag aaggcctttt tggaaaatat atttttaaaa
     1561 ccttctatag gatttagcct ctttaggtct ggttttaaag gctccatggt aaatataaaa
     1621 cctatttggg ccagatttat agccacgcct agaactataa ctataattaa aaagggtaac
     1681 aaaaataagc caaacataat tagatgatca agtaaaagcc tgcttaaatt tttgcttata
     1741 tccaggctaa gattagtatc taaggagttt tttaaataag ttaaaccatt gacataaata
     1801 tactgggaca atagccctgc caaaatagta aagataaaga aggaagctgc cgcatttaga
     1861 tcagcgcttt tggctacctg ccccttttcc ctagcttctc tcagccgttt gggggtagct
     1921 tctt
//
LOCUS       DATGSI010000180         1252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_437361, whole genome shotgun sequence.
ACCESSION   DATGSI010000180 DATGSI010000000
VERSION     DATGSI010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437361"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          388 a          272 c          183 g          409 t
ORIGIN      
        1 catcctttct ttcttatgta ttctttcttt tcctaaaaga tatattcctt tatggacgga
       61 ctcatgtccg tctatgtaat aattattata taatatgtac atatacaaga gagcctctaa
      121 gctctctcta tacttattca tataagtacc gtaggatatc atatcctact tacgagagac
      181 ctcaaagtct ctcttggatt ctatcgaaca tactgaacgg atattcatat atcctaaatt
      241 atccttcata gtataatttg tatattcgat agatgcaatt tccccctaag ttgtttcctc
      301 ggttcaagtt taacgagtgt ctcctataga gtcttttccc gttaagccta agccgaatca
      361 tcaacttcga gagaattctt attctcaaga tatgcaacgt gtattatcag ggctgctgtt
      421 gttaaggtca gtgcaggaaa ctgcttcgta ctctagtgaa atctatctgt tcacacagta
      481 gtctaatact accaactgct agagccttat aggtcagact atcttcatag cctcttgaac
      541 ctgaggtatc ttccctgttt tgctccctag agctgcatac tgcccattgg gaagtgccgg
      601 ggaatggaaa ctaagcatca ccaaagtatc ccaaaagagc acccacaagg caattaattt
      661 ttattgccta tactatatta gacaacaatg tctaagggaa gttccctaaa ttttaaagaa
      721 tgtctaagag aaaaatgtaa gttactgtta ttaaaggaaa gaaaatttca actattttta
      781 cccatattgg ttattttttg accaactcct attggagggc ggtgaaattt gagaatttat
      841 tgtagatatt tttgagttgc ttatatacca tacggaagac ccccatgtcc ccctgtccgg
      901 ccctatgatg attgatgaca ctcgattaaa tcattcaccc ccctacccct tacaatattc
      961 catcataagg tactctaatc atgagattag aataccgata gatggactat ctcatgatag
     1021 tcaatcatac ggaaaattat tccatccgta ttaatatatg tatattattc catcacgtga
     1081 aatattattc catggatgta aattagttga taaagtcaac catacatacg gccattcatt
     1141 ccttctctta tccatcaata cttacactat atacccatac ccttgataca ttcaaacaac
     1201 cattcaacta taccacgtat acatatacac acaagacaca accgcctaac ta
//
LOCUS       DATGSI010000181         3469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_486852, whole genome shotgun sequence.
ACCESSION   DATGSI010000181 DATGSI010000000
VERSION     DATGSI010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486852"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..654
                     /locus_tag="VK031_02825"
     CDS             265..654
                     /locus_tag="VK031_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HLS52878.1"
                     /translation="MDNNERDRNRNVDRDDHFSIGEMILRVLVSSIVIAVAAFFTPGF
                     SVDGLGSILLAAVVIGVMDYLIQRFTGIDASPFGRGIVGFLVAAIILYVTKFIVPGFH
                     ISMWGAIIGALVIGVIDALIPGKGTLH"
     gene            1144..2538
                     /locus_tag="VK031_02830"
     CDS             1144..2538
                     /locus_tag="VK031_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HLS52879.1"
                     /translation="MIKVENVKYTTVKEPLYDLPKRQPELPDKVFEHRLDKVLKSMDS
                     RGLDYILIYADREDYGNFEYLTGFEPRFEEAILVLNKSGKAHLLLGNECIGMAKYSRI
                     KAEGVLYQTFSLPNQPMDENRDLKDILTDIGLNDTHKVGIVGWKLIYPVYGTEDMFDV
                     PALMVDGVKAVAKEENIVNATDLFIHPDYGVRVINTADEIAYFEFGAAYASDAVQKML
                     LNLRTGLTEVELSQYTTAGSLPTSCFPKVISGDRTEINMVSPTTNEIKLGDRFQVTVG
                     LIGGQSNRRGFAAYSEEDLPEGAKDWLEKHTKPYFAVAANWYEQIGIGVTGAEIYDMV
                     EENYPKSKYNWFLNPGHLIATEEWMSSPIYEGSDITIKSGMCFQMDIIPFVEPPYVAP
                     DFEDGIAIADEELRQELKEKYPEVYERIEKRREFMREVLNINIKPEVLPLSNIAGLFR
                     PFMLNKDKALVVDR"
     gene            2734..>3469
                     /locus_tag="VK031_02835"
     CDS             2734..>3469
                     /locus_tag="VK031_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HLS52880.1"
                     /translation="MSEIVKHDQGLMRKSNKILILNLIREKRPISRAELAKITGMSPT
                     SVSRIVSKLMEFGLIEETKLTSNGVGRKASLLDTSELSACSISIYLDLDSCIIGIVDF
                     DGNLLKEERLTYDTENLIWTDIMNRVCETVKEMVKATAADHIKIVGLGVAVPGIVDTH
                     RGVVVFSPQLGWTGVNLKKYLEEELGYRVVIENAIKSKALAEGIYGGAKDSSSAVYIN
                     FGSGVGSALITEDEIFRGTTNSAGEVG"
BASE COUNT         1269 a          476 c          710 g         1014 t
ORIGIN      
        1 gattagacct ccttttaatt catactaata tactagcata tatttaatta tttttctaca
       61 atatattaga tatttttatt tgacctgtct ataagttatc ctaataaata ttctaacaaa
      121 ttttctatta tatactaaag aatctggctg gtaactttta tataaattat cctttataag
      181 ctatataccc aataaatata aaaaatgatt aaattgtata atataccttc tattaggaaa
      241 atactatcct taggaggtga aaatatggat aataatgaaa gagacaggaa taggaatgta
      301 gatagggatg atcattttag tattggcgaa atgatactaa gggtattggt cagttccatt
      361 gtaattgcag tagcagcatt ttttacacca gggttttcag tagacggttt gggcagtata
      421 ctattggcag cagtagtaat aggagttatg gattatctaa tccaaagatt tacaggcata
      481 gatgcatctc cctttggtag aggcattgta ggattcctag ttgctgcaat tatactttat
      541 gttacaaaat ttatagtacc aggtttccat atatccatgt ggggtgctat tataggggcc
      601 ctggttatag gtgtaataga tgctctaatc cctggcaagg ggacattaca ctaaaatata
      661 acttaaattt taaataatcc taagacttaa gtcttaggat tatttagttt atatcttagt
      721 ggtaataaaa ataggcaatc tgaagatttg aaataatatg ggaaatatca agagaatttt
      781 caaaattata ttgacaaatt gagaaagtga ttatatactg ataattaata acaccactgg
      841 agaaattaat ctacatatta atatttcaat atatgttaat atttggccag aagatttata
      901 caagtattta tggtgattac ctaggattgg ccaattccat aaggcagcta ggatgaagga
      961 agaaggccac agcaaagtat ttatggtggc tccaggtgca tgtaacctaa gagttgagga
     1021 tatggaattt aacaatctta ttaggcctat gtatccgctg gataaataaa ttaaggcaat
     1081 ggatcatatc taagagattg taaataaaaa tactagcaat tttaatagga ggaatatatc
     1141 ttaatgatta aagttgaaaa tgtaaaatac acaactgtaa aggaaccttt atatgattta
     1201 cctaaaaggc aacctgaatt gccagataag gtgtttgagc acaggcttga taaggttcta
     1261 aagtctatgg attcaagggg cttagattat atcctaatct atgccgatag ggaagactat
     1321 ggtaactttg aatacctaac aggatttgag ccaagatttg aagaagcaat cctagtacta
     1381 aataaatcag gcaaggccca ccttttatta ggtaacgaat gtattggtat ggctaaatat
     1441 agtagaatca aggcagaagg agtcctgtat cagacctttt cactgcctaa ccagccaatg
     1501 gatgaaaaca gggacttaaa agatatactt acagatatag ggttaaatga tacccataag
     1561 gtaggtatag taggatggaa attaatctat cctgtatatg gaacagagga tatgtttgat
     1621 gtaccagccc taatggtaga tggagtaaag gcagtagcaa aagaggaaaa tatagtaaat
     1681 gctacagacc tatttatcca cccagactat ggagtaaggg ttataaatac agcagacgaa
     1741 atagcctact ttgagtttgg agcagcctat gcttctgatg ctgtacaaaa gatgctttta
     1801 aacttgcgta caggattaac agaagtagaa ctatctcagt atactacagc aggctcttta
     1861 ccaaccagct gtttccctaa ggtaatatca ggagacagga cagagataaa tatggtaagc
     1921 ccaacaacca atgaaataaa gttaggagac aggttccagg taacagtagg cctaataggt
     1981 ggacaaagta atcgtagagg atttgcagcc tattcagaag aagatctgcc agaaggagcc
     2041 aaggattggt tagaaaagca taccaaacct tactttgcag tagcggccaa ctggtatgaa
     2101 caaataggaa taggagttac aggagcagaa atctatgata tggtggaaga aaactatccc
     2161 aagtctaaat acaactggtt tttaaaccca ggtcacttaa tagcaacaga ggagtggatg
     2221 tcttcaccaa tttatgaggg ttcagacata accataaaaa gtggaatgtg cttccaaatg
     2281 gacataattc cttttgtaga accaccttat gtggctccag actttgaaga tggaatagcc
     2341 atagccgatg aagaattaag gcaagaatta aaagaaaagt atccagaagt atatgaaaga
     2401 atagaaaaaa gaagagagtt tatgcgtgaa gtattaaata taaatataaa accagaagta
     2461 ctacctttgt caaacatagc aggactcttc cgtccattta tgttaaataa ggataaggca
     2521 ctggttgtag acagataatg tgctttaata taggataagt aaaatttaca aaagataaaa
     2581 atatagcacc ttttacaggt gctatatttt tatgtcagat aatttatgat aatattatat
     2641 aagaaagaat acatcagatt atttgtatat aaattgacaa acaaatttag gttctataca
     2701 ctatattcaa atataagttg gaggattaaa gatatgtcag agattgtgaa acatgatcag
     2761 gggctgatga gaaaatctaa caagattctt atactaaatt taatcagaga aaaaaggccc
     2821 atatctagag cagaactagc taaaattaca ggtatgagtc ccacatcagt aagcaggatt
     2881 gtatccaaac ttatggaatt tggtttgatt gaggaaacaa aactaacatc caatggggta
     2941 ggaaggaagg cttcactatt agataccagt gaactttcag cctgcagcat tagcatatat
     3001 ttagatttag attcttgtat aataggcatt gttgactttg atggcaatct attaaaagaa
     3061 gagaggctca catatgatac agaaaattta atatggacag atataatgaa tagggtttgt
     3121 gaaactgtta aagaaatggt taaagccaca gctgctgacc atataaaaat agtaggtcta
     3181 ggtgtggcag tgcctggaat tgttgatacc cataggggag tggtggtctt ttcgccacaa
     3241 ttaggttgga ctggggtaaa tttaaaaaaa tatttagagg aagaattagg atacagggta
     3301 gtaattgaaa atgcaattaa gtctaaggcc ttggcagaag ggatttatgg aggagcaaag
     3361 gattctagta gtgctgttta tataaatttt ggttctggtg taggctcagc attgattact
     3421 gaggatgaaa tctttagagg aaccacaaac agtgcaggag aagttggac
//
LOCUS       DATGSI010000182         1102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_343175, whole genome shotgun sequence.
ACCESSION   DATGSI010000182 DATGSI010000000
VERSION     DATGSI010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343175"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1102
                     /locus_tag="VK031_02840"
     CDS             <1..>1102
                     /locus_tag="VK031_02840"
                     /inference="COORDINATES: protein motif:HMM:NF022985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HLS52881.1"
                     /translation="DIIVSITLRNMGVNTVSTVNIEWTDGEDTYTEEFSNLDLSTLEE
                     VELTLDSVLNYSSSGQREITVTAIDVNGEDDDNPDDNEVSDIIFVYSDGVEQKIVIEE
                     GTGTWCGWCPRGFVAMDKMYDNPDQFPNFIGIGVHNGDPMTVPEYDNGLGLTGFPGSK
                     INRVLQDVEVDFSEWAQAYAQLEDNIKPFDVSLEVDYDKPERFATINVDAKFFIGLED
                     ADIRLSIVVIEDGVTGTGVGWGQRNFYAGGAAGPMDGWENLPDPVTDFEYDRVARALI
                     GGFNGESGSIPTEIEEGETYSYEFTYTHPEGMNPQKMSFVALLIDQETGEILNAAEEP
                     IPYELGLDKNIVDNFKLYPNPASDVVNATFNET"
BASE COUNT          387 a          133 c          245 g          337 t
ORIGIN      
        1 aagatataat tgttagtatt acattaagaa atatgggggt taatactgtt tctacagtaa
       61 atatagagtg gacagatggt gaagacactt atacagaaga attttcaaat ttagatttat
      121 ctactttaga ggaagtagaa cttactttag attctgtact taattattca tcaagtgggc
      181 aaagagaaat tacagtgacc gcaatagatg ttaatggaga agatgatgac aatcctgatg
      241 ataacgaggt ttctgatatt atatttgtat atagtgatgg agtagaacaa aaaatagtta
      301 ttgaagaagg aacaggaact tggtgtggat ggtgcccaag aggctttgtt gctatggata
      361 aaatgtatga taatccagat cagtttccaa actttatagg aattggtgtt cataatggag
      421 atccaatgac agtacctgaa tatgacaatg gtttagggtt aacaggtttt ccaggcagta
      481 aaattaatcg agttttacaa gatgtggaag tagatttttc tgaatgggca caagcatatg
      541 cgcaattaga agataatata aaaccatttg atgtaagcct tgaggttgac tatgataagc
      601 cagaaagatt tgctactatt aacgtagatg caaagttttt tataggatta gaagatgctg
      661 atattcgttt atctatagta gtaattgaag atggtgtaac tggaactggc gtaggctggg
      721 gtcaaagaaa tttttatgca ggaggagcag ctggtcctat ggatggttgg gaaaatttac
      781 ctgatcctgt tactgatttt gaatatgacc gtgttgcgag agcgcttatt ggaggattta
      841 atggagaaag tggaagtatt ccaacagaaa tagaagaagg agaaacttat tcttatgagt
      901 ttacttacac acatcctgaa ggaatgaatc ctcaaaaaat gtcttttgta gctttattaa
      961 tagatcaaga aacaggagaa attttaaacg cagcagaaga gccaattcct tatgaattag
     1021 gattggataa aaatatagta gataacttta aactttatcc aaacccggct tctgatgtag
     1081 taaacgctac attcaatgaa ac
//
LOCUS       DATGSI010000183         3182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_392608, whole genome shotgun sequence.
ACCESSION   DATGSI010000183 DATGSI010000000
VERSION     DATGSI010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392608"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1791)
                     /gene="infB"
                     /locus_tag="VK031_02845"
     CDS             complement(<1..1791)
                     /gene="infB"
                     /locus_tag="VK031_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HLS52882.1"
                     /translation="MTKIRIYELAQELNMSSKDLIDKLRELDLEVSSHMNSLEADEAE
                     LIRGIYGSGDSETEFDQHGVDNTKDTKTETSETEKHIIEIEESIIVRELADELGVNPS
                     EIITKLIGMGVMANQNQSIDVKTATIIADEFGAIVVTKEVEEEKEEVFELDFEDKPED
                     LVPRPPVVTVMGHVDHGKTSLLDAIRETSVTKSEAGGITQHIGAYSVKVDDKEIVFLD
                     TPGHEAFTSMRARGAQVTDLAILVVAADDGVMPQTVEAINHAKAADVPILVAINKIDK
                     PTANIDRVMQELAENNLLPEDWGGDTITVPVSARDKTGIDDLLEMVLLLAEMQELKAN
                     PNRKPVGIIVEAQLDRGKGPLATVLVQKGTLRVGDMVVSGTSHGRVRAMLDDKGDRVE
                     EAGPSTPVVILGMSEVPNAGDLLYGVKDEKTARSYAESKKEELREAQLRASSQVSLDD
                     LFERIQEGQIKDLNIIIKADVKGSMEALAQSLEKLDTGEEVRINIIHGGVGGITESDV
                     MLASASNAIIIGFNVRPNLNAIEVSKTEKVDIRTYRVIYEAIEDIQAAVKGMLAPELV
                     EKVLGRAEVRATFRLPNGSTIAGVYILDGKV"
     gene            complement(1820..2098)
                     /locus_tag="VK031_02850"
     CDS             complement(1820..2098)
                     /locus_tag="VK031_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HLS52883.1"
                     /translation="MRKRRVPLRKCVSCGEKRAKQELIRIVKTTEGDFILDPSGRING
                     RGAYICPSVGCFEKGIESKRLSKAFREEVPKEVLDTLKIEIEEFIKGV"
     gene            complement(2112..3158)
                     /gene="nusA"
                     /locus_tag="VK031_02855"
     CDS             complement(2112..3158)
                     /gene="nusA"
                     /locus_tag="VK031_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HLS52884.1"
                     /translation="MNEDFIHALDEIEREKGISRDVIFEALESALISSYRKNFGSSQN
                     VVVEMDKVTGEANVFAIKDVVDEVEDELLEIDLEEARKLNSSFEVGDKVKLEITPKDF
                     GRIAAQTAKQVVIQRIKDAERDVIYEEFINRENEIITGMVQRISRNNVFIDLGKTEGI
                     LPPSEQIEGEIYEQSDRLKLLIHEVRKTTKGPQIILSRSHPDLIKRLFELEVPEISNG
                     VVEIYSIAREAGSRTKIAVFSKDPNVDPLGACVGFKGSRVKAIVDELTGEKIDIVIWS
                     NDIEEFIANSLSPSKVLKVEADEEEKSALVVVPDYQLSLAIGKEGQNARLAAKLTGWK
                     IDIKSESQYEEAIE"
BASE COUNT          817 a          790 c          418 g         1157 t
ORIGIN      
        1 taccttacca tctaggatat atactccagc aatggtgctt ccatttggta gcctaaaggt
       61 agcccttact tcagccctgc ctagtacctt ctccactagt tcaggtgcca acattccttt
      121 gacagcagcc tgaatatctt ctatagcttc atatattact ctataggttc tgatgtccac
      181 cttttcagtt tttgatactt caatggcatt taaattaggt cgtacattga atccaattat
      241 tatagcgttg gaagcggatg ctaacataac atcactttct gttattccac ctacgccccc
      301 gtgaataata ttgattctta cctcttctcc agtgtctaac ttttccaagg attgagctaa
      361 ggcttccatg gatcctttaa catcagcctt gattattata tttaaatcct taatttgacc
      421 ttcctgaatt ctttcgaata aatcatctaa agatacctgg ctacttgccc ttaactgtgc
      481 ttcacgcaat tcttcttttt tgctttcagc ataggaacgg gcagtttttt catccttaac
      541 tccatacaat aaatcgccag cattaggaac ttctgacata cctaaaatta ccaccggtgt
      601 agatgggcct gcctcttcta ccctgtctcc cttatcatct aacatggccc taactctacc
      661 atgggaggtt cctgaaacca ccatatctcc aacccttagg gtaccttttt gcaccaatac
      721 tgtggccaag ggccccttgc ccctatctaa ttgggcctca actataatcc caacaggttt
      781 tctattagga ttagccttta actcttgcat ttctgccaac aataatacca tttccaatag
      841 atcatctata cctgttttat ccctagcaga tactggtaca gttatagtat ctccacccca
      901 gtcttctggt agtagattat tttctgccag ttcttgcata accctatcta tatttgcagt
      961 aggtttatct atcttgttta tagcaactag tataggtaca tctgctgctt tagcatggtt
     1021 gatggcctca actgtctgtg gcattacccc atcatctgcc gcaaccacta aaatagctaa
     1081 atctgttact tgggcccctc ttgctctcat ggacgtaaaa gcctcatgac caggagtatc
     1141 taaaaatact atctccttgt catctacttt aacagaatag gctcctatat gttgggttat
     1201 accccctgct tcactttttg taacacttgt ttcacgtatt gcatccaata gggaagtttt
     1261 tccatgatcc acatgcccca taactgttac cacaggtggt ctgggcacta gatcctctgg
     1321 cttatcctca aagtctaatt caaatacttc ttccttttct tcttcaactt ctttggttac
     1381 aacaattgcc ccaaactcat cagctattat tgtagctgtt ttcacatcta tgctttgatt
     1441 ttgatttgcc ataaccccca taccaataag tttggttata atttctgatg gatttactcc
     1501 cagttcatct gccagttccc taactattat actttcttcg atttctatta tgtgtttttc
     1561 tgtttctgaa gtctcagtct tagtatcctt tgtattgtcc actccatgct gatcaaattc
     1621 agtttcagaa tcacctgaac cataaatacc tctaatcagt tctgcctcat cggcttccaa
     1681 tgaattcata tgactgctga cctccagatc taattctctt aacttgtcta ttaaatcttt
     1741 actgctcata tttagttctt gagctaattc atatattcta atttttgtca tatactgcac
     1801 ccccatttaa ttttagtgtc tatacccctt tgataaattc ttctatttct atctttaagg
     1861 tatccaacac ttccttcggg acttcttctc taaaagcctt agatagtctt ttagattcaa
     1921 ttcctttttc aaaacaccct acacttggac aaatataggc tcctcttcca ttaatcctac
     1981 ctgaaggatc aagaataaaa tcaccctctg tggtttttac tattctgatc aactcctgtt
     2041 ttgctctttt ttcaccgcag gatacacatt ttctaagtgg tactctcctc tttctcattt
     2101 tatcacctca attattcaat agcttcttca tattgagatt ctgatttaat gtctattttc
     2161 caaccagtca gcttggcagc taacctggca ttttgtcctt ctttacctat tgctaatgat
     2221 aattgatagt caggaactac cactaaagct gatttttctt cttcatcagc ttccactttt
     2281 aaaactttgg aaggacttaa actgttggct ataaattctt caatatcatt actccaaata
     2341 acaatatcta ttttctcacc tgttaattca tctacaatcg ccttcaccct gcttccctta
     2401 aaaccaacac aggcccccaa tggatctaca ttaggatcct ttgaaaatac tgctatcttt
     2461 gttctagagc ctgcctctct tgcaattgaa tatatctcca caactccatt gctgatttct
     2521 ggcacttcca gctcaaatag gcgctttatc aggtccgggt gagagcggga taaaattatt
     2581 tgtggtccct ttgtggtctt tctaacttca tgaataagta gcttcaatct atcagactgt
     2641 tcatatatct ctccttctat ctgctcagat ggtggcagaa ttccctcagt ctttcccaaa
     2701 tcaataaaaa cattatttct tgaaatcctt tgaaccatac ctgttattat ttcattttct
     2761 cggttaataa attcctcata aataacatct ctttcagcat cttttatcct ttgtataact
     2821 acttgcttag ctgtctgggc agcaattcta ccaaaatcct ttggagtaat ttctaactta
     2881 accttatcgc ccacctcgaa ggaactattt aatttacgtg cttcctctaa gtctatctct
     2941 aaaagttcat cttccacttc atctaccaca tcttttatgg caaatacatt ggcttctcct
     3001 gttactttat ccatttctac aaccacattt tgtgatgaac caaagttttt cctataacta
     3061 gaaattagtg ccgattccaa ggcttcaaat attacatccc ttgatatacc cttctccctt
     3121 tcaatttcat caagagcatg tatgaaatct tcattcataa cctaacctcc tatattttta
     3181 ta
//
LOCUS       DATGSI010000184         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_60070, whole genome shotgun sequence.
ACCESSION   DATGSI010000184 DATGSI010000000
VERSION     DATGSI010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60070"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VK031_02860"
     CDS             <1..717
                     /locus_tag="VK031_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52885.1"
                     /translation="DDKVVEDLGFAFIKEGLKSVRTGLGDDQAEAKEGYFQAANNVLV
                     MIGEKTSGNLSAEDFKETADFLASPEFAFFVKEGGLNAESAEVASRVFKQVYSSNIDG
                     QVKKFLKSELPAKVDGQTLRYSDVISIEYRDGRVRLTPKQITPDDPQGNVQEIARNIN
                     SSVRLQERAINQNVRIGAHLEGTTDYRKFWEENKDILMPYVFKDPNTEIPDQEYNGQM
                     YRYIGGPTNSRSSWEPIDAE"
     gene            707..1249
                     /locus_tag="VK031_02865"
     CDS             707..1249
                     /locus_tag="VK031_02865"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLS52886.1"
                     /translation="MLNNLPPWEKSWGRGEIEYEDPPIPPWEKEWGRKPNINVEGFSP
                     IMQEGSLRFDDVFNKLIQAESNWRHRDEEGNLITSPVGARGIAQIMPKTAKDPGYGVK
                     PLQDDSEEESLRFGRDYLSALVKHFNGDYRKAVAAYNAGPGNVKRAVRLSKKSGKDWT
                     EHLPKKEETIPYMKKILGEA"
     gene            1249..1581
                     /locus_tag="VK031_02870"
     CDS             1249..1581
                     /locus_tag="VK031_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HLS52887.1"
                     /translation="MARKGQYKPGAKKSSVRQRKYNSSPAQKKRRAQRNAARRKMERA
                     GAVRKGDGKEVDHKDKNTANNSRSNLRVRNKSANRADNRGTGGRKPATKKAAKKMAKK
                     TTKRKTTK"
     gene            1578..1874
                     /locus_tag="VK031_02875"
     CDS             1578..1874
                     /locus_tag="VK031_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52888.1"
                     /translation="MNYIDAQVKYINSVLGTEYNNLVSATKAYYEYALKEGLPGAVGP
                     EGPEGPQGSEGPEGPQGPEGPEGPQGPAGNDGFPTETQWNDLVSRVEALENPEG"
     gene            complement(1927..2076)
                     /locus_tag="VK031_02880"
     CDS             complement(1927..2076)
                     /locus_tag="VK031_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52889.1"
                     /translation="MTEELIDELAKYIVDNQYGLYMLDHVSGTYQLSYEELRLKIKEF
                     YNINK"
BASE COUNT          760 a          395 c          533 g          551 t
ORIGIN      
        1 gatgataagg tagtagaaga cctaggcttt gcctttatta aagaaggatt aaaaagtgtc
       61 agaacaggct tgggtgacga tcaagccgaa gccaaagagg gctattttca ggcggccaat
      121 aacgtattgg ttatgattgg ggaaaagact tctggaaact taagtgctga ggattttaag
      181 gagacggcag atttcctagc atctcctgag tttgcattct tcgtaaaaga gggaggatta
      241 aatgccgaat ctgcagaggt agcctctagg gtatttaagc aagtctattc atctaatatt
      301 gatgggcagg ttaagaagtt tctgaagtcg gaactacctg ctaaagtaga tggacaaacc
      361 ctgaggtatt ctgatgtaat cagtatagag tatagggacg gaagagtaag actaactcct
      421 aaacaaatta cacccgacga ccctcaagga aacgtccagg aaattgctag gaatataaat
      481 agctcggtta ggttacaaga aagagctata aaccaaaacg taagaatagg ggcgcattta
      541 gaaggcacaa cagattatag gaagttctgg gaagaaaaca aagacatatt gatgccttat
      601 gtttttaaag accctaatac agaaatccca gatcaagaat ataatggaca aatgtatagg
      661 tacattggtg ggcctactaa cagccgtagt agttgggagc ctattgatgc tgaataattt
      721 accgccatgg gaaaagtctt gggggagagg agagattgaa tatgaggacc ctcctatccc
      781 tccttgggag aaagagtggg ggaggaagcc taacataaat gtagaaggtt tttcccctat
      841 tatgcaggaa ggatctctga gatttgatga cgtatttaat aaactaatcc aggcagagag
      901 taattggagg catagagatg aggaagggaa tttaataaca tcccctgtag gggctagagg
      961 aattgcccaa atcatgccta agactgctaa agatcctggt tacggggtta aacctttaca
     1021 ggacgattca gaagaagaaa gcttaagatt tggtagggac tatttatccg ccctagttaa
     1081 acattttaat ggggattata gaaaggccgt ggccgcatat aacgcagggc caggtaatgt
     1141 gaagagagct gttagattgt ctaagaaatc tggtaaagat tggaccgagc atcttccaaa
     1201 aaaagaagag acaatccctt atatgaagaa gattttagga gaagcttagt ggcaagaaaa
     1261 ggtcaatata aaccaggagc taagaagtcc agtgtgcgtc agaggaagta caacagtagc
     1321 cccgctcaaa agaagaggag agcacaaagg aacgctgcta gacgtaaaat ggaaagagct
     1381 ggagctgtta gaaagggaga tggtaaagag gttgaccata aagataagaa cactgctaat
     1441 aactctaggt ctaatttgag agtgaggaat aagtctgcca acagagcaga taatagaggc
     1501 acaggaggcc gcaagcctgc tactaagaaa gctgccaaga aaatggctaa gaagacaacc
     1561 aaaaggaaaa cgactaaatg aactatattg atgcacaagt gaagtatata aactcagtac
     1621 tggggacaga atataataat ctagtatctg ccactaaagc gtattatgaa tacgcactta
     1681 aagagggtct tccaggtgct gttggcccag agggccccga aggaccacaa ggatcagagg
     1741 gcccagaagg tcctcaaggc ccagagggcc ccgaaggacc acaaggcccc gcaggtaatg
     1801 atgggttccc tacagaaaca caatggaacg atttagtttc tagggttgaa gcgttagaaa
     1861 acccagaagg ttaaacgaca aaaaccccct tggattttta agtccttggg ggtttatttt
     1921 tatctattac ttattaatat tatagaactc ttttattttt aatcttaatt cctcatagct
     1981 tagttgataa gttccggaca catgatctag catgtatagc ccgtattgat tatccactat
     2041 atactttgct aattcatcta taagttcttc tgtcatctgc tatgcctcac aagctaaaca
     2101 atctccgcta actaccaccc cactcctact gtaaatataa tagatagata ggatgttttc
     2161 gtccataaaa gctttagaga taacttccga tattctctct tcatctccat cttctgatac
     2221 gaagaagttt agggattgt
//
LOCUS       DATGSI010000185         1264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_13815, whole genome shotgun sequence.
ACCESSION   DATGSI010000185 DATGSI010000000
VERSION     DATGSI010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13815"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..>1264
                     /locus_tag="VK031_02885"
     CDS             252..>1264
                     /locus_tag="VK031_02885"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52890.1"
                     /translation="MLYEIVEHFPNEIIYEGKAPCISLYQPTYRHKPESRQNIIRFKN
                     LIQRIQNSLLNKYSKDETNKLLKPFYTIAEDRLFWNESKDGLAILSNKDKTIVYRLNR
                     PVKEFAVVSDSFHIKPLLRIYQSADRYHLLGISRKNFSIYEGDRYGFEKIELEEDIAT
                     SIEEVLGDQYTEPHLNLGAYGGATGSYMYHGHGSRKDEIDKDTERFFRYVDKLVLDKF
                     SNPEKIPLILIGLTEHHGLFRNITSNPHLMEDGILKDYEALDIEEMRNEAWKVMEPYY
                     LNKTKELVDRFKMEQAKFLATDDLAQIARAILEGRVDTLLVEADKIYPGKINEETGEI
                     IRD"
BASE COUNT          436 a          194 c          238 g          396 t
ORIGIN      
        1 ccaattttaa ttttcttctc tactatatga tctgtttcta agtgtttttt catcagcatc
       61 cccttcctat ctacaatatg ttataagttt cttgtcatgc cttacattta tattacttgt
      121 taaaaattat aagtgaacta gattataagt taccctgaat atatttaatt aatcctaaac
      181 cagccttctt ttaaaaaaac ttagcttggg tatataatta ataatcacca ttaatatata
      241 ggagggattc tatgttatat gaaattgttg agcatttccc taatgagatt atttatgaag
      301 gaaaagctcc ctgtatttct ctttatcagc ctacatatag gcataaaccg gaaagtaggc
      361 aaaatattat tagatttaaa aatctgatcc aaagaatcca gaattcccta ctaaataaat
      421 actctaaaga tgaaacaaat aaacttctca aaccattcta taccattgca gaagataggc
      481 tattttggaa tgagtccaag gatggtttgg ctattttatc taataaggat aagactattg
      541 tatataggtt aaataggcct gttaaggaat ttgctgtggt ttctgatagt tttcatatta
      601 aaccacttct tagaatatat caatctgccg atagatatca ccttttaggt attagtagaa
      661 aaaacttttc catctatgaa ggcgatagat atgggtttga aaaaatagag ctggaagaag
      721 acatagctac cagcattgaa gaggtcttag gtgatcagta tacagaacca catttaaatt
      781 taggggccta tggtggcgct acagggtcct atatgtatca cggccatggt agcaggaagg
      841 atgaaataga caaggatact gaaaggtttt tcaggtatgt ggacaagttg gtcttagata
      901 aattttcaaa tcctgaaaag attcccctaa ttttaatagg gcttacagaa catcatggac
      961 tctttagaaa tataactagt aatccccacc taatggaaga tggaatttta aaagactacg
     1021 aggctctaga tatagaagag atgagaaatg aggcctggaa ggttatggaa ccctactatt
     1081 taaacaagac caaagaactg gtagataggt ttaaaatgga gcaagccaag tttttagcta
     1141 cagatgatct ggcccagatt gctagagcca ttcttgaagg ccgggtagat accctgctgg
     1201 tggaggcgga taagatttat cctggaaaaa ttaatgagga aactggagag attattagag
     1261 ataa
//
LOCUS       DATGSI010000186         1036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_295041, whole genome shotgun sequence.
ACCESSION   DATGSI010000186 DATGSI010000000
VERSION     DATGSI010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295041"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1036)
                     /locus_tag="VK031_02890"
     CDS             complement(<1..>1036)
                     /locus_tag="VK031_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HLS52891.1"
                     /translation="GIGQLYNIYRDPNIITGFGLGASSIALFARVGGGIYTKAADVGA
                     DLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAGMGADLFESYVGSIISAITLGLVAY
                     GESGATFALALSAVGIIASIIGVYFVRGDGDPQKSLNNGTLVSSLVTVVAGYFLSSYI
                     LDSHKPFVAILSGLAVGLIIAKITEYYTSADYEPVQRIAKESETGASTNIIGGLSVGM
                     MSTAGPIVIIAIGILIAYYVAGGSTNVSEGLYGIALAAVGMLSTAGMTIAVDAYGPIA
                     DNAGGIAEMCELPDEVREITDTLDAVGNTTAAIGKGFAIGSAALTALALFASYTQAVG
                     LTGIDLTTPTV"
BASE COUNT          310 a          252 c          172 g          302 t
ORIGIN      
        1 taactgtagg agtagtcaag tctataccag taaggcctac tgcctgagta taggatgcaa
       61 acaaggctaa agccgttaaa gctgctgaac caatggcaaa tccttttcca atggctgctg
      121 tagtatttcc tactgcgtct aaagtatctg taatttccct tacttcatct ggcagttcac
      181 acatttcagc aattccacca gcattatctg caattggtcc gtaggcatct actgctatag
      241 tcattccagc tgtggacaac atgcctactg ctgctaaagc aattccatat aaaccttcag
      301 atacatttgt gctaccacct gccacataat atgctattaa aatgcctatg gctatgataa
      361 ctatgggacc ggctgtagac atcataccta cagataaacc accaataata ttggttgacg
      421 cacctgtttc tgattctttt gcaatccttt ggactggttc gtaatctgcc gatgtatagt
      481 attctgttat tttagctata attaaaccta ctgctaaacc agataaaatg gccacaaaag
      541 gcttatgtga atctaaaata taagaactta aaaagtatcc agcaaccact gtaacaagag
      601 aacttaccaa agttccattg tttaatgatt tttggggatc tccatctcct cttacaaaat
      661 aaactcctat aatagaagct attataccta ctgctgacaa ggctaaagca aatgtggctc
      721 cactttctcc ataggctact aagcctaaag ttatggctga aataatggat cccacatagg
      781 actcaaatag gtcggcaccc atgcctgcca cgtcccccac attgtctcca acattatctg
      841 caataacggc agggtttcta gggtcatcct ctggaattcc ggcctctacc ttacctacta
      901 ggtcggcccc tacatctgct gcctttgtat atattcctcc acctacccta gcaaataagg
      961 caatggaaga agctcccaat ccaaatcctg ttataatatt tggatcccta taaatattat
     1021 aaagttggcc aattcc
//
LOCUS       DATGSI010000187         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_157260, whole genome shotgun sequence.
ACCESSION   DATGSI010000187 DATGSI010000000
VERSION     DATGSI010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157260"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VK031_02895"
     CDS             <1..302
                     /locus_tag="VK031_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HLS52892.1"
                     /translation="AIRGVPLIFGKPMIKDLFLDLLDGLGGSLRTSYDTRLEKIMKLA
                     CTNAIKSGDKIGSVEIVALYKDLLKCDNPHTCPHGRPTIIELSKKDIEKQFLRIN"
     gene            313..1254
                     /gene="miaA"
                     /locus_tag="VK031_02900"
     CDS             313..1254
                     /gene="miaA"
                     /locus_tag="VK031_02900"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HLS52893.1"
                     /translation="MKEKLFVLTGPTAVGKTSLSIALAKKLGGEIISADSMQIYKYMD
                     IGTAKVKAEEMEDVPHHLIDIVYPDEEFTVSDYQKQATKLIKELNHKGRLPIIVGGTG
                     LYINSLVYELQFAKVPPNEELRQRYEELANTYGNEYLHEKLSRIDEESSQRIHVADRK
                     RIIRALEIYEETGKTMTEYNKNFRKETNKYDLAMVCLNMDRAQLYERINKRVDLMLND
                     GLVEEVKVILKMGYDKESVALQGIGYKEIIMYLDGQISLDRAVELIKQGSRNYAKRQL
                     TWFRRDKRIHWVDVGDFDGFEELINYLTKHVNDKIKP"
     gene            1271..1489
                     /gene="hfq"
                     /locus_tag="VK031_02905"
     CDS             1271..1489
                     /gene="hfq"
                     /locus_tag="VK031_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HLS52894.1"
                     /translation="MKAIANLQDFFLNQARKERVGVTIFLVNGYQIKGYVQGFDNYTI
                     ILEGEGKQQLIYKHAISTIIPDTEIVFQ"
     gene            complement(1535..>2016)
                     /gene="lexA"
                     /locus_tag="VK031_02910"
     CDS             complement(1535..>2016)
                     /gene="lexA"
                     /locus_tag="VK031_02910"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HLS52895.1"
                     /translation="HLEKLESLGYIRKDATKPRAIEIIERDEDFLLTRKKTVDVPIIG
                     QVTAGAPILAVENIEDTYPVPADLVEGLDVFMLEIKGESMVDAGILDGDLVLVREQKN
                     ANNGDIVVALMDDEATVKRFFKEEDRIRLQPENEYMDPIYVRDVGILGKVIGLYRKL"
BASE COUNT          693 a          320 c          389 g          614 t
ORIGIN      
        1 tagctataag gggagttccc ttaatttttg gaaaacctat gatcaaagac ctgttcttag
       61 acttactaga tggtctaggg ggcagcctta gaaccagtta tgatactagg cttgaaaaaa
      121 taatgaaact tgcttgtacc aatgccataa aaagtggtga taaaattggt agcgtagaga
      181 ttgttgcttt gtataaagat ttactaaagt gtgacaatcc ccatacctgt ccccatggaa
      241 ggccaacaat tattgagtta agtaaaaaag atatagaaaa acaattttta agaattaact
      301 aaggatgata gaatgaaaga aaaattattc gtacttactg gacctacagc agtaggaaaa
      361 actagccttt ctattgcttt ggcaaaaaag ctaggtgggg agattatttc agcagactct
      421 atgcaaatat ataagtacat ggatataggg acagccaagg ttaaagctga agagatggaa
      481 gatgttcccc accacttgat agatattgtc taccctgatg aggagtttac tgtttcagat
      541 tatcaaaaac aggctacaaa gcttattaaa gagcttaatc ataagggacg gcttcctatt
      601 atagttggag gtacagggct ttatattaac tcacttgtct atgaactgca atttgctaaa
      661 gttcctccca acgaggaatt aagacaaagg tatgaggagt tggctaatac ttatgggaac
      721 gaatatttac acgaaaaatt aagcaggata gatgaagaaa gtagccaacg aatccatgtg
      781 gcagatagaa aaaggataat tagagcttta gagatatatg aagaaacagg gaaaaccatg
      841 actgagtaca ataaaaactt ccgtaaagaa actaataagt atgacctagc tatggtttgt
      901 ctcaacatgg acagggcaca attatatgaa agaataaata aaagagtaga tcttatgttg
      961 aatgatggcc tagttgaaga ggttaaggta attctgaaaa tgggatatga caaagaatca
     1021 gttgctttgc aaggaattgg gtataaagag attataatgt atcttgatgg acagataagc
     1081 cttgatagag cagttgagtt aataaaacag ggtagtagaa attatgctaa acgtcagcta
     1141 acctggttta gaagagataa gagaatacat tgggttgatg taggggattt tgatggtttt
     1201 gaggagttaa ttaattattt aacaaagcat gtaaatgata agataaaacc ataggatggg
     1261 aggagtcgtt ttgaaagcta ttgcaaattt acaggatttt ttcttgaatc aagccaggaa
     1321 ggaaagggta ggtgttacca tatttttagt caatggttat cagattaaag ggtatgttca
     1381 aggatttgat aattatacta ttattttaga aggtgaaggt aagcagcagc taatatataa
     1441 acatgccata tcaacaatta tacctgacac tgaaatagtt tttcagtaat caaagtcaaa
     1501 taaagccctc ctggagggct ttatttgact ttatttataa tttcctatat aggccgatta
     1561 ctttacctaa tatgcctaca tctcttacat atataggatc catatattca ttttccggtt
     1621 gtaatcttat cctatcttct tccttgaaaa atcttttcac agtggcttca tcgtccatta
     1681 aagccactac tatatctcca ttgttagcat ttttttgctc tctaaccaac actaaatctc
     1741 cgtccaaaat acctgcatct accatgctct caccttttat ttctagcatg aaaacatcta
     1801 aaccttccac taaatctgca ggtacagggt aagtatcttc tatattttcc actgccaaaa
     1861 taggggcccc tgccgtaact tgccctatta taggcacatc aactgttttt ttcctagtta
     1921 ataagaaatc ttcatccctt tcaatgattt caatagccct aggttttgta gcatcctttc
     1981 taatataacc tagtgattct aatttctcca gatggg
//
LOCUS       DATGSI010000188         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_437808, whole genome shotgun sequence.
ACCESSION   DATGSI010000188 DATGSI010000000
VERSION     DATGSI010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437808"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VK031_02915"
     CDS             complement(<1..616)
                     /locus_tag="VK031_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52896.1"
                     /translation="MKFKFYPKESRLYDFLLFPRLVFYIEEYKKNSKDYNFGQLISDD
                     YLDFIRKVEAELLPYKEEIEEFYYMHDYSRYDFIELVFRLNNIFGYKDEISYLDMLEG
                     LEENEIKISIVYSIMSYNEDFQLYSKEVIKHTKNIVSSEEKLISFIKDLPIDSSIKWD
                     LFVVLKSPKDHMNKYVSLMKKIFPIFNQIYISYEGKINEYGKYLT"
     gene            complement(606..1814)
                     /locus_tag="VK031_02920"
     CDS             complement(606..1814)
                     /locus_tag="VK031_02920"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLS52897.1"
                     /translation="MQQFALSLYVLAITGSATIFASMLSISILPRLLLSPIAGVFGDW
                     FDRKRTIVVLDLINSFIIGSFALIYIVRGGLSLRMIYILVILLEITEIFFDSAMSAVL
                     PSLVDKDELLEANSFNSLVMNIGNILSPVIASLLYGTFGMKVVLIINSISFLLSAISE
                     IFINIPKNHKAPEHINFKSFKKDLVEGINIIKGNKLISTMISLGTIINFCAVPLFSIG
                     LIFIIKEVLKLTDYQFGIFQMIMSIAMLIAPLVMGLIKDIKIGRLCYLSFTLVSLLIL
                     VMAIIPSSPLLNSFSSSLMPFILLSVVSFIIGVLVTVANIGIGTLFNEIVPLELMGRT
                     STVFNFAVTIFIPLGQMLFGALYDIMPASIVIGLAGVLKLIAIMKYKRTLLQYDLEET
                     DSIGGIIDEI"
     gene            complement(2185..2673)
                     /locus_tag="VK031_02925"
     CDS             complement(2185..2673)
                     /locus_tag="VK031_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HLS52898.1"
                     /translation="MRYLVRQKIFAFSDRFTIKDENQLPRYQVVGKLLTLGNKLDLYD
                     MDGNHLIYIEQKLFRFLPEYLIYKEGTVLARIKKEFTFFKPRFSIESQYGDFTLDGNI
                     LAHDFNILKNGRIVAWISKRWFSFADTYTVDIVDEEDHAFILSLAIVIDQVFHDNNHN
                     NN"
     gene            2790..3410
                     /gene="ytaF"
                     /locus_tag="VK031_02930"
     CDS             2790..3410
                     /gene="ytaF"
                     /locus_tag="VK031_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HLS52899.1"
                     /translation="MKCILLVLAISLDAFVASIAYGSKKIKIPLLSTLIINIICSLLL
                     AVSVLLGSQLKRVLPGNITALASFLILLLLGIFYLFEGLTKNYLWSRVDFNKEIELKL
                     SDINFIINIYLDQTKADYDYSKKLDYKEALYLGFALSLDSLTIGFGSGLTDINYAYLL
                     GLSLIVGLISIRAGLLIGEKLAIRSKLNLSWLAGVILIFLALSRLF"
BASE COUNT         1189 a          613 c          483 g         1221 t
ORIGIN      
        1 cagttagata ttttccatac tcattgattt ttccctcata agatatatat atctgattga
       61 aaatggggaa tatttttttc attaagctta catacttatt catatgatcc ttaggagatt
      121 tcaacactac aaacagatcc cactttatgc tagaatcaat aggcaaatct tttatgaaag
      181 aaattaattt ttcctcacta ctaactatat ttttagtatg ttttataact tcttttgagt
      241 aaagttgaaa atcttcatta tatgacataa ttgaataaac aatactaatt tttatttcat
      301 tttcttctaa cccttctagc atatctaaat aagatatttc atccttatag ccaaatatat
      361 tatttagtct aaaaaccaac tcaataaaat catacctgct ataatcatgc atatagtaaa
      421 attcttctat ttcttcttta taaggaagta gttcagcttc aaccttcctg atgaaatcaa
      481 ggtaatcatc cgatattaat tgcccaaaat tataatcctt tgagtttttt ttgtattctt
      541 ctatataaaa tactaatctt ggaaatagaa gaaaatcata tagcctactc tcctttggat
      601 aaaacttaaa tttcatctat tatacctcct atggaatcag tttcctctaa atcgtattgt
      661 aataatgttc ttttatactt cataatagca ataagcttaa ggactcctgc tagacctatt
      721 actatgcttg ctggcattat atcataaagg gctccaaata acatttgtcc cagtggaata
      781 aaaattgtaa ccgcaaaatt gaacactgtt gaagtccttc ccatcaattc caaggggaca
      841 atttcgttaa acaaggtccc tatgccaata ttggcaacag ttacaagaac acctattatg
      901 aatgagacaa ctgataatag tataaagggc atcaaactgg aactaaaact atttaataaa
      961 gggctggaag ggattattgc cataactaat attaataaag atactaaggt aaaacttaag
     1021 taacaaagtc tacctatttt tatatccttt attaaaccca taactaaagg tgcaattagc
     1081 attgcaatag acataatcat ttgaaagata ccaaattgat aatccgtcag ctttaatact
     1141 tccttaatta taaatattaa tccaatagaa aagaggggta ctgcacaaaa attgattatg
     1201 gtacctaaac ttatcatggt agaaataagc ttatttcctt taataatatt tattccttca
     1261 actaaatcct ttttaaagga cttaaaattt atatgttcag gtgccttatg gtttttcggt
     1321 atgttaataa agatttcact aatggcagaa agtagaaagc ttatggagtt tattatcaat
     1381 actaccttca tcccaaatgt cccatataaa agagaggcta taactggaga tagtatattt
     1441 cctatattca ttactaaaga gttaaaagaa ttagcctcca ataattcatc cttatctact
     1501 aagctaggta gcactgcaga catggcagaa tcaaaaaata tttcagtaat ttccagtaat
     1561 attactaata tataaatcat cctaagagat aagccacccc taactatata aattaaggcg
     1621 aagcttccta taataaatga attaattaag tctaatacta ctatggtcct tttcctatca
     1681 aaccagtctc caaatactcc tgctatagga gataaaagta gtcttggcaa aattgaaatg
     1741 gatagcatag aagcaaaaat agtggcagaa cctgtaatgg ccaaaacata tagggataga
     1801 gcaaattgct gcatattgct tcacaataaa gacacaagct ttcctaaaat cagtaaagaa
     1861 aagtcttttt gttttaaaag ttcaaaattc ataaacaatc ctccataatt tattctttac
     1921 cttattatat gtaggtatat gtctaatgtc aatgattatg tttgatgttc atctaatccc
     1981 taatatgcat ttagatttgt ttaaatagtt tcttatgaaa ttctctattt caagtgaatt
     2041 ttttatcata atatgtactt cctggtagaa acatgaaaaa accatagggt tccctaccta
     2101 ttgaacacag gcaaggggcc ctatggttta aaatattact ataagtctag attttagaat
     2161 ttacatcata ctgaaagaag taatttaatt attattatga ttgttatcat gaaacacctg
     2221 gtctattaca atggctaagg ataatataaa ggcatgatcc tcttcatcca ctatgtccac
     2281 agtataggtg tctgcaaagc taaaccatct tttacttatc caggctacta tcctgccatt
     2341 ctttaatata ttgaaatcat gggccaaaat attcccatct agggtaaagt caccatactg
     2401 gctttctata gaaaacctgg gcttaaaaaa ggtaaattcc ttttttatcc tggccaggac
     2461 agtcccctcc ttatatataa ggtactcagg taaaaatcta aataatttct gttcaatgta
     2521 tattaaatga ttcccatcca tgtcgtacag atctaatttg tttccaaggg tcagcaattt
     2581 acctaccacc tgatatcgtg gaagttgatt ttcatcctta attgtaaatc tatctgaaaa
     2641 agcaaatatt ttctgtctta ctaaatatct catgctacta cctcctatat gcttttatct
     2701 tacttcattt tgatagtcct gtgttaaaat ataacaatta tttgtctcct gaatactatg
     2761 attatatcat taaagaagga gtgtagcata tgaaatgtat cctattggtc ctagccatat
     2821 ctttagatgc attcgtagca agtattgcct atggctcaaa gaaaataaag attccccttc
     2881 tatctacctt aataattaat ataatctgct ccttgcttct tgccgtgtct gttttattag
     2941 gttcccaact taagagagta cttcctggca atattaccgc cttagctagt ttccttatac
     3001 ttttattgct aggaatattt tatttgtttg agggcctaac taaaaattat ctatggtcta
     3061 gggtagattt taataaggag atagagttaa aattgtcaga tataaatttt ataattaata
     3121 tataccttga ccaaaccaag gcagactatg actattctaa aaaattagat tataaggaag
     3181 ccctatacct tggctttgcc ctttctttag attccttaac aattggcttt ggcagtggcc
     3241 ttacagatat aaattatgcc tatcttttag gcctatcttt aattgtgggc ctaatttcta
     3301 taagggcagg gcttttaatt ggagaaaagt tggccatcag gtctaagcta aacctatcct
     3361 ggctggcggg agtgatccta atatttttag ccctatccag gttgttctaa taaactggct
     3421 tgagaacagg ccagtttatt cattctcctc taccctaata tatagcttta acacctctga
     3481 taaaattact ttctcagctg ttagct
//
LOCUS       DATGSI010000189         1158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_60447, whole genome shotgun sequence.
ACCESSION   DATGSI010000189 DATGSI010000000
VERSION     DATGSI010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60447"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VK031_02935"
     CDS             complement(<1..582)
                     /locus_tag="VK031_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLS52900.1"
                     /translation="MYDLIIIGAGVSGASIARRLSRYKLDILVLEKQIDVSMGASKAN
                     SAIVHGGYAESHDELRGRICYPGRLEFNNLEKELGFGFLENGSLVLAFDKEDEKVLEK
                     LYDQGIENGLDDMEIIGKDKLKKIEPNVSDEAISALYCKGAGVCSPYEYVIALMENAI
                     ENKVKLKLKSEVVDIKKDEDRFKVDTADGNTYEG"
     gene            complement(597..>1158)
                     /locus_tag="VK031_02940"
     CDS             complement(597..>1158)
                     /locus_tag="VK031_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HLS52901.1"
                     /translation="ACIFGALTGAHFNPALTIALALDGQVDWANVPIYAISQLLGAFC
                     GAVIVYILFKDHYEETSNEPETVRGTFCTAPSIPNTFRNLFSEIVGTFILVFAIIGFG
                     NVGGAATAGVDKIFVYGLIVSIGMSLGGLTGYAINPARDLGPRLAFAVLPFTNKADPN
                     WGYAWIPIVGPIIGGVLAVILFNFIF"
BASE COUNT          352 a          261 c          178 g          367 t
ORIGIN      
        1 tccctcataa gtgtttccat ctgctgtgtc tactttaaat ctatcctcat cttttttgat
       61 atctacaact tcacttttaa gctttaattt gaccttattt tctatggcat tttccataag
      121 ggctatgaca tattcataag gagagcatac cccagctccc ttacaataaa gtgctgatat
      181 tgcctcatct gatacatttg gctctatctt ttttagctta tcttttccaa ttatttccat
      241 atcatcaagg ccattttcta taccttggtc atatagtttt tctaaaactt tttcatcttc
      301 tttgtcaaaa gctaaaacca aggacccatt ttccaaaaat ccaaagccta gctctttttc
      361 tagattatta aactcaagcc ttccaggata gcaaatccta ccccttagct catcatggct
      421 ttctgcatag ccaccgtgga ctatggcaga gtttgccttg cttgctccca tggatacatc
      481 gatttgtttt tctaaaacta agatatcaag cttgtatctt gaaagccttc ttgctataga
      541 tgccccgctt acgccagcac ctattattat taaatcatac ataaaatcct gtctttctaa
      601 aatataaagt taaatagtat tactgcaagc acgccgccaa ttataggtcc tacaattgga
      661 atccaagcat agccccaatt tgggtcagcc ttgtttgtaa aaggaagtac agcaaaggca
      721 agccttgggc ccaaatctct tgcaggattt atggcatagc ctgtaaggcc acccaaactc
      781 atacctattg aaactataag accatataca aaaatcttat ctactccagc tgttgcagct
      841 ccaccaacat ttccaaatcc tattatagca aaaactaaaa taaaagttcc aactatttct
      901 gaaaataagt ttctaaaagt attaggaata gaaggtgctg tacaaaaagt gccccttaca
      961 gtttcaggct cattggatgt ttcttcatag tggtctttga ataaaatata gacaataaca
     1021 gccccacaaa atgctcctaa aagttgggat atagcataga ttggaacatt ggcccagtct
     1081 acttgaccgt caagggcaag ggcaatggtt aaggctgggt taaaatgtgc cccggttaag
     1141 gctccaaaaa tacaggca
//
LOCUS       DATGSI010000190         1623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_582010, whole genome shotgun sequence.
ACCESSION   DATGSI010000190 DATGSI010000000
VERSION     DATGSI010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582010"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="VK031_02945"
     CDS             complement(<1..1527)
                     /locus_tag="VK031_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HLS52902.1"
                     /translation="MGKTEFKEKKKRFNLKRFRLAGKKKASKLNLNSLGFKLIIFFSI
                     LILLLSSSIGVTILQRASNSTIAREEQYLASIAYEGAKLINSRVETQLGVLTTIAARD
                     DIQTMDWEVQQPILQEEVDRTDFMDLAIVNMNGETLYASGETTNLADMVYVIRALSGG
                     ANVSDLIIDRVSKEIVLMFAAPIEREGRVVGALIGSQDGNTLSQMVNDIGHTEDGYGY
                     MISRTGEVVAHPDKDLVLNQFNPFEEAKEDETLESLAEFFESTKNRQPGTANYTFQGD
                     ELYAGYAPVENTSWTCVYVTNKEDFLRPVAILRRSIFNIVAISLVVGVILVYMAGKYI
                     VNPVTLATKYSNKLALLDIEEDLSERLIKRKDEIGDLAKAFQNIINNLRNIIGEVSES
                     SELVSSTAEELTATSQDSASSAEEINKVVEEIAKGAAEQVSNIEEGVSKARILGEAIE
                     KDGEYLEGLNTASGKVALIVDEGLVEMDRLASIADDSDDAIERIHDVILNTNDSAIKI
                     G"
BASE COUNT          422 a          384 c          254 g          563 t
ORIGIN      
        1 acctatttta atggcactat cattggtgtt taaaattaca tcatggattc tttctatagc
       61 atcgtcacta tcatcagcaa tactagctag cctgtccatt tctaccagcc cttcatctac
      121 tattagagcc accttgcctg aagccgtgtt tagtccttct aaatattctc catccttttc
      181 tatggcctct cctaggattc tggccttgga tacaccttcc tcaatattag atacctgctc
      241 cgctgctccc ttggctattt cttctactac cttgttaatt tcctctgcag aagaagcaga
      301 atcctgggag gtggctgtaa gctcctctgc agtagaagat actaattctg aagactcact
      361 tacctcacct attatatttc ttaaattatt tattatattt tgaaaggcct tggccaggtc
      421 tccaatttcg tcttttcttt taatcagcct ttctgacaag tcttcctcta tatctaaaag
      481 ggccaactta tttgaatatt tggtagccaa agttacaggg tttactatgt atttacctgc
      541 catatatact aatatgacac ctactaccag gctgatagct actatattaa aaatgcttct
      601 acgtaagatg gctaccggcc ttaaaaagtc ctccttgttt gtaacgtaca cacaggtcca
      661 gctagtattt tcaactggtg catatcctgc atataattca tcgccctgga aggtgtagtt
      721 ggctgtgcca ggctgacgat ttttggtgga ctcaaagaat tctgccaagc tttctagagt
      781 ctcatcttcc ttagcctctt caaaaggatt aaattgatta agtaccaagt ctttatctgg
      841 atgggctaca acttcacctg ttctactgat catgtaaccg tagccatctt ctgtatggcc
      901 tatatcattt accatttggc ttaaggtgtt tccatcctgg ctacctataa gggccccaac
      961 taccctgcct tccctttcta ttggggcagc aaacattagg acaatttctt tgcttacccg
     1021 atcaataatt aaatctgaaa cattggctcc accacttaag gccctgatta cataaaccat
     1081 atcagccagg ttggtggttt caccgctggc gtataaggtc tccccattca tgtttacaat
     1141 tgccagatcc ataaagtctg tcctatctac ctcttcctgc aaaataggct gctggacttc
     1201 ccaatccata gtctgaatat catctctggc tgctattgta gttaaaacac ctagttgcgt
     1261 ctctactcta ctattaatta gcttggcccc ttcgtaggct atggaggcta ggtattgctc
     1321 ctcccttgct atagttgaat tactggccct ctgtaaaata gtaactccta ttgaacttga
     1381 tagcagtaaa attagaatag aaaaaaatat gattaattta aagcccaaac tgttgaggtt
     1441 cagtttagag gccttctttt ttccagctaa tctaaatctt tttagattga accttttttt
     1501 cttctcctta aactctgttt tccccatctt aaccctccca taaaatttct ttatctatat
     1561 caatgaagct attaagatta taaaattaaa cttcctgtta gaatcttctt tcaatttata
     1621 tcg
//
LOCUS       DATGSI010000191         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_487564, whole genome shotgun sequence.
ACCESSION   DATGSI010000191 DATGSI010000000
VERSION     DATGSI010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487564"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2078
                     /locus_tag="VK031_02950"
     CDS             <1..2078
                     /locus_tag="VK031_02950"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HLS52903.1"
                     /translation="SESQERMAVVIDRKDLNFFIKEAEEEDLEATLVAQITEEKSLKM
                     YWQGKEIVNIKREFLATNGVSKKIGAQINQPKGKGLLAEDLDKFTKLPLKEAIFKMLG
                     DLNIGSQKGMIEKFDNTAGANTVLMPYGGENQLSPSEGMVGRIPVLKGKTNTCSIMTY
                     GYEPKISQWSPFHGAYYAVIESIAKVVALGGDYRKIRFTFQEYFERLGEDENKWGKPL
                     AALLGAYLVQKKLGLASIGGKDSMSGSFEDLHVPPTLISFAITTEKIDKIISNEFKGA
                     NNKIVLLPLEMDENHLLDFEQLKATYIRIKQLIDKNIILSAQSIKYGGVARALCEMAF
                     GNKIGFDIEMDKASLFKPLFGSIVVEISADVDLEDVFSNIRYQLLGRTRPDGVVKING
                     EVMELDSMIKAWEEPLMDVFFVTDKKMKRETRVQVREPLIRLGKRIAKPRVLIPIFTG
                     SHGEYTTGQAFERAGAQVETFVFKSLNKKQLKSSYKYFAKKIRNSQIIALPHGSILAN
                     EPESGGKLIRLIFTNPYIKEEINKHLKIRDGLILGIGDGFQALLKTGLIDKGQVLDTG
                     DTTSYLASNRSGSFISTMVDVEVTSKLSPWMNEMELGHIYTTALACKEGQIKIKDEKI
                     LREGQAACRFVASNPTGSIENIEALTSPDGRVLGTISAIDRQDGALYKNIEIKGEHKI
                     FQSGVNYFK"
BASE COUNT          754 a          308 c          495 g          550 t
ORIGIN      
        1 tatctgagtc tcaagaaaga atggctgtag taatcgatag gaaggattta aacttcttta
       61 taaaagaggc ggaagaggaa gatttagagg ccaccttggt agcccaaata acagaagaaa
      121 aaagccttaa aatgtactgg cagggtaagg aaattgtaaa tataaaaaga gaatttctgg
      181 ctaccaatgg ggtaagcaaa aaaatcggag ctcagataaa tcagcctaag ggaaaggggc
      241 ttctagctga agatttagac aagtttacaa aattgccctt aaaagaggca atctttaaaa
      301 tgttaggaga tttaaatatt ggaagccaga agggtatgat agaaaaattt gataatactg
      361 caggtgcaaa tacagtccta atgccctatg gaggagagaa tcagctgagc cccagcgaag
      421 gtatggtagg cagaatacct gtattaaaag gaaagaccaa tacctgttcc ataatgacct
      481 atggatatga gcctaaaata agtcaatgga gtccctttca tggagcttat tatgcggtta
      541 ttgagtcaat cgccaaggtg gtagccctag gaggagatta cagaaaaata cggtttacct
      601 tccaagagta ctttgaaaga ttaggagaag atgaaaacaa gtggggcaag ccattggcag
      661 ccctactggg ggcctacctg gtacaaaaaa agctaggcct ggccagtata ggcggaaaag
      721 atagtatgag tggtagtttt gaagatttac atgtaccgcc taccctaata tcttttgcca
      781 taacaacaga aaaaatagac aagataataa gcaacgaatt taaaggggca aataataaga
      841 tagttcttct gcctttagag atggatgaaa accacctact agattttgaa caactaaagg
      901 ccacttatat taggattaaa caattaatag ataaaaatat tattttatct gcccaaagta
      961 ttaaatatgg aggcgtggcc agggccttat gtgaaatggc ctttggaaac aaaataggct
     1021 ttgatataga aatggataag gctagtttat ttaagccctt atttggttcc attgtggtgg
     1081 agatttcagc agatgtggat cttgaagatg tgttctcaaa cattagatat cagctgctag
     1141 gtaggactag gccagatggg gtggttaaaa ttaatggaga agtaatggaa ttagatagta
     1201 tgattaaggc ctgggaagag ccccttatgg atgtgttttt tgtgactgat aagaagatga
     1261 aaagggaaac cagggtacaa gtgagagagc ctttaattag acttggaaag agaattgcca
     1321 agccaagggt tttgattcca atattcactg gtagccatgg tgaatacact acaggccagg
     1381 cctttgaaag ggcaggggcc caggttgaaa catttgtttt taagtcctta aataaaaaac
     1441 agcttaagtc aagttataaa tactttgcta agaaaataag aaatagccaa attatagcct
     1501 taccccatgg ttcaatcttg gccaatgagc ctgaaagtgg aggtaaatta ataaggctta
     1561 tctttactaa tccctatata aaagaagaaa taaacaaaca tttaaagata agagatggtc
     1621 tgattttagg tataggagat gggttccagg ctttattgaa gacaggtttg atagacaaag
     1681 ggcaggtttt agatacagga gatactacct cctatttagc cagtaacagg tcaggtagct
     1741 ttatatctac tatggtagat gtggaagtta cgtctaagtt atctccctgg atgaatgaaa
     1801 tggagctagg ccatatttac acaaccgcct tagcttgtaa ggaggggcaa ataaagataa
     1861 aggatgaaaa gatattaagg gaaggccagg ctgcctgcag gtttgtagcc agtaatccta
     1921 caggctccat agaaaatata gaggccttaa ccagtcctga tggaagagtg ttaggcacta
     1981 tatctgccat tgatagacag gatggagcct tatataaaaa tatagagatt aaaggagaac
     2041 ataaaatatt tcagtcaggt gtaaattatt ttaaatagga ggtttaaaat gaaagtaagt
     2101 atagtaa
//
LOCUS       DATGSI010000192         1127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_295522, whole genome shotgun sequence.
ACCESSION   DATGSI010000192 DATGSI010000000
VERSION     DATGSI010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295522"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /gene="fliY"
                     /locus_tag="VK031_02955"
     CDS             <1..963
                     /gene="fliY"
                     /locus_tag="VK031_02955"
                     /inference="COORDINATES: protein motif:HMM:NF005995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HLS52904.1"
                     /translation="ATTLSSILNHKVLITTPRVSIVTFEQIVEECSIPKVVANVEFKS
                     GITGNNFLMVDVPDAAIIADLMMGGNGENAKEELTELELSAIAEAMNQMIGSASTSMA
                     TMIGEQVDINPPEISLWDREDNLETGLIDTEDYICRIAFNLTVEGLIESEIMQIFTLE
                     AVHNISKVMLGQSEEDKQAELDDTSSSQEEKVEYMEESKDVGGLGQTEESREEKIELK
                     RPEFPPLSPSSDQERPPRNLDLIMDVPLEFSVVLGKSRKTIKEVLSLSNGSIVELNKL
                     ADEPLEIYVNGKLIAEGEVVVINENFGIRITNILSKEQRVNNLK"
BASE COUNT          428 a          164 c          253 g          282 t
ORIGIN      
        1 gctacaaccc tatcttctat acttaatcat aaggttttaa taactactcc cagagtatct
       61 attgtaacct ttgaacaaat agtagaagag tgtagcattc caaaagtagt ggccaatgta
      121 gagtttaaaa gtggcattac aggtaataac ttccttatgg tggatgtacc agatgctgcc
      181 attatagcag atttgatgat gggcggtaac ggagaaaatg ccaaggaaga attaacggaa
      241 ctagaattaa gtgccatagc tgaggctatg aaccaaatga taggatctgc ctccacctcc
      301 atggctacca tgataggaga gcaggttgac ataaatccac cagagatttc cctatgggac
      361 agggaggata atctggagac aggactaatc gatacagagg attatatctg tagaatagcc
      421 tttaacctaa cagtggaagg gctaattgaa agtgagatta tgcaaatctt taccttggaa
      481 gctgttcata atatttcaaa agtaatgtta ggccagagtg aagaagataa gcaagcagag
      541 ttagatgata caagctctag tcaggaagaa aaggtggaat atatggaaga gagtaaggat
      601 gtaggcggtc taggacaaac agaagaaagc agagaagaaa aaatagaatt gaaaagacca
      661 gaattcccac cattaagccc aagttcagat caagaaaggc cacctagaaa tctagacctt
      721 attatggatg tgcccttgga attttccgtg gttctaggta aaagtaggaa gacaattaag
      781 gaggtattat ccttaagcaa cggatccata gttgaattaa ataagctggc agatgaaccc
      841 ttggagattt atgtgaacgg aaaactaata gccgagggag aagtggtggt tataaatgaa
      901 aactttggca ttaggattac aaatatatta agcaaggaac agcgggttaa taatttaaaa
      961 taaacctatt aaagaatatg aaaaagcacc gatatattta gtaataggta tattaggaga
     1021 gattttatga agattaataa gccatataaa atccagggta agtatttaga caagataaat
     1081 aaatcctctg atgaaaggga gaaaaatctt tcaaatgctc aaaatga
//
LOCUS       DATGSI010000193         3263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_438237, whole genome shotgun sequence.
ACCESSION   DATGSI010000193 DATGSI010000000
VERSION     DATGSI010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438237"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1713)
                     /locus_tag="VK031_02960"
     CDS             complement(115..1713)
                     /locus_tag="VK031_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52905.1"
                     /translation="MMKRLVHLLILFTLSLLLIGCKSLTQNPLLFKASDLEDKEDSRD
                     EEEVIDFNIEKISLSKSYQNIDPDVEIAKKGSNYKLLASLGLVESSGVKVKKVSKTGN
                     QVNIYVENRSNKDRQLVVPNIIIDLGEFDPGNPDDIKFNIVNENYKPIKIRLSANEAI
                     NRVNADCKISTNTLPHIDIERQNDKYYWKLDYYHIFDRSTVDNPIINLFVVVDADNGD
                     IITLDKDSISSYIDDGHILDFIPHKYILYKKVEERSKSDPIETLWMYNIENQEKRFLY
                     TSSEGIISAKFHPEEDKIAFLEKHKRNNQLFIIELTNERPYKVSFQLPINPRIIQWKD
                     KNQLYLVDRLENKSIIYEYSLVTDTSVKRFDVDKEILDLKVQGDYFLVLEEDLKSEET
                     KISLTKNFRRSIYNDFGTMPQFINNNQISYIKSEGKKDRLYIYDIEDWELKDPLNLNV
                     SNYFPLADDTLVVTEKNQNKKDYTLYEYNLKKDKLISIINIGDDKIILNQVDNKLYVN
                     TAIPDRDESTHIIYSVDLSKTSDH"
     gene            1847..>3263
                     /gene="uvrB"
                     /locus_tag="VK031_02965"
     CDS             1847..>3263
                     /gene="uvrB"
                     /locus_tag="VK031_02965"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HLS52906.1"
                     /translation="MNKFKIHSEFKPTGDQPKAIDQLVDGINNNLKHQVLLGVTGSGK
                     TFTMANVIEQVQKPTLVIAHNKTLAYQLASEFKEFFPENAVEYFVSYYDYYQPEAYVP
                     HTDTYIEKDASINEEIDKLRHSATAALFERRDVIIVASVSCIYGLGDPIDYENLVVSL
                     RPGMIKDRDEVMKKLVDIQYMRNDINFVRGTFRVRGDVLEIFPAASSENTIRVEFFGD
                     EIDRIVEVNHLTGEVIGIRKHVSIFPASHYATTEDKIKRALISIEEELEERLKELRDQ
                     DKLVEAQRLEQRTRYDMEMLEEMGFCSGIENYSRHLSNRPAGSRPFTLIDYFPEDFLI
                     IVDESHVTIPQIRGMYEGDRSRKTNLVEHGFRLPSALDNRPLKFHEFESLINQIVHVS
                     ATPGPYEYEHSQNTVEQIIRPTGLLDPVIEVRPTKHQIDDLISEIKKVVDRDERVLIT
                     TLTKKMAEALTKYFIEIGIKVT"
BASE COUNT         1106 a          516 c          570 g         1071 t
ORIGIN      
        1 aatcccttga taaaagaagg ataaggatag aatattttaa ttttagaaaa gggctggtga
       61 aggtagccta tgaaggggat aaaaaaacct taggaatcaa aggtcttcct aaggttaatg
      121 atcagatgtc ttagataaat ctacggaata gataatatgg gtactttcat ccctatctgg
      181 aatagcagta ttaacatata gcttattatc aacttggttt aagataattt tatcatcacc
      241 aatattaata atagatatta acttatcttt cttaaggtta tattcgtaaa gggtataatc
      301 tttcttattt tgattttttt cagtaacaac taaagtatca tctgccaaag gaaagtaatt
      361 agaaacattt aggtttagag gatcttttaa ttcccaatcc tctatatcat aaatatatag
      421 gcggtctttt ttcccttcag actttatgta tgatatttga ttattgttaa taaattgagg
      481 catggtgcca aaatcattat atatagacct tctaaagttc ttagttaagg aaattttagt
      541 ctcttcactt tttaaatctt cctctaggac caagaaataa tccccttgaa cctttaaatc
      601 tagaatctct ttatccacat caaatctttt tacactggta tcagtaacca agctatactc
      661 atatataata gatttatttt ccagcctatc aactaaatat aattggtttt tatccttcca
      721 ttgaataatt ctagggttga ttggaagttg gaaactgacc ttatagggcc tttcatttgt
      781 aagttcaatt ataaatagct ggttgtttct tttatgtttc tctaagaagg caattttatc
      841 ttcttcagga tgaaatttag cactgattat accttcactg gaggtatata aaaatctttt
      901 ctcctggttt tcaatattat acatccatag agtttctata gggtctgact ttgatcgttc
      961 ttcaaccttc ttataaagga tatatttatg tggtataaaa tctaaaatat gtccatcatc
     1021 aatatatgaa gagattgagt ctttgtccaa ggttataata tctccattat ctgcatccac
     1081 aactacgaat aaattaatta taggattatc cacagtagat ctatcaaata tgtggtaata
     1141 atctaatttc caataatact tgtcattttg tctttcaata tcaatatgtg gtaaggtatt
     1201 agtagaaatc ttacaatctg catttaccct attaattgct tcattggcac ttaatctgat
     1261 tttaataggc ttgtaatttt catttactat attaaattta atatcatcag gattacctgg
     1321 gtcaaattct cccaaatcaa ttataatatt aggtacaacc aattgtctat ctttgttact
     1381 cctattttcc acatagatat taacttgatt acctgttttg gaaacttttt taaccttaac
     1441 tccagatgat tcaactagac ctaaggaagc taatagttta tagttggaac ctttcttagc
     1501 aatctccaca tcagggtcta tattctgata ggatttagaa agactgattt tttctatatt
     1561 aaaatcgata acttcttctt catccctgct atcttccttg tcctcaagat cagatgcttt
     1621 gaaaagtaag gggttctgtg ttaagctttt gcaaccaatt aaaagtaagc ttaaggtaaa
     1681 taaaatgagc aagtgcacaa gtcgtttcat catattcact ccttaaatca tattctacaa
     1741 tataaaatac ttttctacaa gaaaatatta tataatatgt tttgacaata tatttaaata
     1801 gtatataata ttgccatgaa cagacgttca aacagaggtg agtaaagtga acaagtttaa
     1861 aatacattct gaatttaaac ctacaggcga tcagccgaaa gctattgatc aattggtaga
     1921 tggaataaat aataatctaa aacaccaagt cttattaggt gtgacaggtt ctggaaaaac
     1981 atttaccatg gccaatgtaa tagagcaggt gcaaaaacca accctggtta tagcccataa
     2041 taagacttta gcctatcaac tggccagtga atttaaggaa ttctttccag aaaatgctgt
     2101 agaatacttt gtaagttatt atgattatta ccaacctgag gcttatgtac ctcatacaga
     2161 tacttatata gagaaagatg cttcaattaa tgaggaaatt gataaacttc gccactcggc
     2221 aactgctgct ctctttgaga gaagagatgt aattatagtt gcatcagtat cctgtatata
     2281 cggactggga gatccaatag attacgaaaa cttggtagta tctcttagac caggtatgat
     2341 aaaggatagg gacgaggtta tgaaaaagtt agtagatatt cagtatatga gaaatgatat
     2401 aaattttgtt aggggaacct ttagagtaag gggagatgtg ttagaaatat tcccagctgc
     2461 atccagtgaa aacacaatta gggtggagtt ctttggagat gaaattgatc ggatagttga
     2521 ggtaaaccat ttgacagggg aagttatagg aattagaaaa catgtgtcta tctttccagc
     2581 ctcccactat gctacaacag aggacaagat aaagagagcc cttatatcta ttgaggagga
     2641 attggaagag cggttgaaag aattaaggga tcaggacaaa ttagttgagg cccaaagatt
     2701 ggagcaaagg accagatatg acatggagat gctggaagaa atgggctttt gttcaggcat
     2761 agaaaactac tcaaggcact taagtaacag accggcaggc agtaggccct ttaccttgat
     2821 agattatttc cctgaagact ttctaattat agttgatgag tcacatgtta ctataccgca
     2881 gattaggggt atgtatgagg gggaccgatc tagaaaaact aatttggtgg aacatggctt
     2941 taggctacca tcagctttag acaataggcc tttaaaattt catgagtttg aatctttaat
     3001 aaaccaaata gttcacgtgt ctgcaacgcc aggcccttat gaatacgagc atagccagaa
     3061 tactgtggaa cagattataa ggccaactgg cctactggat cccgttatag aggtaagacc
     3121 aactaagcat caaatagacg acctaatctc ggagattaaa aaggtggttg atagggatga
     3181 gagggtactt ataaccacct taactaaaaa aatggcagaa gcccttacta aatactttat
     3241 tgaaatagga attaaagtaa ctt
//
LOCUS       DATGSI010000194         1714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_110340, whole genome shotgun sequence.
ACCESSION   DATGSI010000194 DATGSI010000000
VERSION     DATGSI010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110340"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VK031_02970"
     CDS             <1..661
                     /locus_tag="VK031_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HLS52907.1"
                     /translation="GRQAYIVCPLIEENDTLDLNSAEELYQKLKSHVFKDYRLGLLHG
                     KMSAKEKDEIMKDFKDGLIDILVSTTVIEVGVDVPNANIMVIYNAERFGLAQLHQLRG
                     RVGRGQYQSYCILINYGQGKIARERVRVLESSSDGFKISEKDLELRGPGEFFGTKQHG
                     LPELRIANLFAHMDILKVAQKEAMEIVKSDPNLDKKENQPVKKKVAELFEKIEQDIVL
                     N"
     gene            731..1288
                     /gene="rsmD"
                     /locus_tag="VK031_02975"
     CDS             731..1288
                     /gene="rsmD"
                     /locus_tag="VK031_02975"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HLS52908.1"
                     /translation="MRVISGNRKGHRLKGPKNKKIRPTEDRIKESLFNILGPINPDAL
                     VLDLFAGSGSIGIEFLSRGAKKAYFVDISSASISLIKENLQNTKLMEKATVMKRDSIR
                     AIKCFKDKSTRFDYIYIDPPFREDKLIFDALEVIDNTNILADKGIIIIEHWEGLGFED
                     KYLGYKNIDCRKYGSKSITFFAPNS"
     gene            1300..>1714
                     /gene="coaD"
                     /locus_tag="VK031_02980"
     CDS             1300..>1714
                     /gene="coaD"
                     /locus_tag="VK031_02980"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HLS52909.1"
                     /translation="MKVIYPGSFDPVTYGHLDIIERCANKFDHVIIAVLNNVSKKSTF
                     SIDERIRLLEETTKHLDNIEVDSFSGLLADYAEKKKCNTMIRGLRAVSDFEYEMQMAL
                     VNRKMNENIETLFMVSSSEYVYLSSSIVKEIASYGG"
BASE COUNT          639 a          212 c          363 g          500 t
ORIGIN      
        1 tggtaggcag gcctatatag tttgtcccct gattgaggaa aatgacacct tagatttaaa
       61 ttctgctgaa gaactttatc aaaagcttaa aagccatgta ttcaaagatt atagactagg
      121 cctgttacat ggaaaaatgt cggccaaaga gaaagatgaa attatgaaag attttaagga
      181 tggcctaata gatatactag tgtctactac tgtaattgaa gtaggagtag atgtaccaaa
      241 tgcaaatatt atggtgatct ataatgcaga aaggtttggc ttagcccaac tacatcaatt
      301 aagaggtagg gtagggaggg ggcagtatca gtcctactgt atcttgatta actacggtca
      361 agggaagatt gctagagaaa gggtaagggt gttagaaagt tcatctgatg gttttaaaat
      421 ctctgagaag gatctggaac ttagagggcc aggagaattt tttggaacta agcaacatgg
      481 acttccagag ttaagaatag ccaatttgtt tgctcacatg gacatattaa aggtggctca
      541 aaaagaggct atggagatag taaaatcaga tcctaattta gataaaaaag aaaatcaacc
      601 tgtaaaaaag aaggtagctg aattatttga aaaaattgaa caagacattg tattaaatta
      661 acttatatta ctggttataa tatgataaaa taagagcaga tattttatta ttgaaataag
      721 gagttaaaca ttgagagtta tatctggaaa tagaaagggt cataggctta aaggacctaa
      781 aaacaaaaaa attaggccaa ctgaagatag gataaaagag tccttattta atatattggg
      841 ccctattaat cctgatgctt tagtactaga tctatttgct ggttcaggtt ctataggaat
      901 cgaattttta agtagagggg caaaaaaagc ttattttgtt gatatatcat cagccagcat
      961 atccttgatt aaagaaaatt tacagaacac taaattaatg gaaaaagcca ctgttatgaa
     1021 gagggatagt attagggcta ttaaatgttt taaagataag tctacaagat ttgattatat
     1081 ttatatagat cctcccttta gggaagataa gcttattttt gatgccttgg aagttataga
     1141 taatacaaat atattggctg ataaagggat cattataata gaacattggg aaggactagg
     1201 atttgaagat aagtatttag gatataaaaa tatagattgc agaaaatatg gtagcaaatc
     1261 aattacattt tttgctccaa atagctagga ggtaaaagga tgaaggtaat atacccaggc
     1321 agctttgatc cggtaacata tggccatctt gacattattg aaaggtgtgc caacaaattt
     1381 gaccatgtaa taatagcagt tttaaacaat gtttcaaaga aatctacctt tagtatagat
     1441 gaaagaatca ggttattgga ggagacaaca aaacacttgg ataatattga agtagattct
     1501 ttctctggac tgctggctga ttatgcagag aaaaagaaat gtaacaccat gattagaggg
     1561 ttaagggctg tatctgactt tgaatatgag atgcagatgg ccctagtcaa tagaaaaatg
     1621 aatgaaaaca tagagaccct atttatggta tctagcagtg aatatgttta tttaagctcc
     1681 agcatagtca aagaaatagc gtcctatgga ggca
//
LOCUS       DATGSI010000195         1233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_60933, whole genome shotgun sequence.
ACCESSION   DATGSI010000195 DATGSI010000000
VERSION     DATGSI010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60933"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..471)
                     /locus_tag="VK031_02985"
     CDS             complement(205..471)
                     /locus_tag="VK031_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52910.1"
                     /translation="MNNNKLYRRKEAYELRSVTQQDIEEYEEKGYITSHETSDNVIKV
                     LIKHSDEENGSPKIGDMIAKSTKNMLHQWLIEEESFKSGWEKVR"
     gene            complement(528..1052)
                     /locus_tag="VK031_02990"
     CDS             complement(528..1052)
                     /locus_tag="VK031_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52911.1"
                     /translation="MIYKIIAVYLISVVLPLLVIGKQKETLKLEGDYYPLVYLSFFPF
                     VNSVFLASLVVSYVCFKLSDFIVFLQTHRSNNRKLLEDLIEYVKKENISGHLDMKLDE
                     YYTTKILTVYEDGEVRLNMVNIKNSLNRRQGRKLFKEMKKGFREQEGYTEENKVREER
                     EAIEKFYKERINEN"
BASE COUNT          334 a          249 c          136 g          514 t
ORIGIN      
        1 acttcccatt aattttaggg agggggtagc aatagggagt atcccgtgta tatgtccatt
       61 taatacttta aacttataat atttttttta attttttatt ccattttcaa cctacagcct
      121 attctctgta tatgtcttct ctctattatg tattgatctt acttctctgt aaggttactc
      181 tttggttagc cgagcattta cattctacct tactttttcc catccacttt taaaggattc
      241 ttcttctatt aaccactggt gtagcatatt ctttgtactt tttgcgatca tatctcctat
      301 cttagggctt ccgttctcct cgtctgaatg ttttatcagt actttaatta cattatctga
      361 tgtctcatgt gatgttatgt atcctttttc ttcatattct tctatgtctt gttgagttac
      421 gcttcttagt tcgtatgctt cttttcttcg gtatagtttg ttattgttca tgttatttta
      481 tattaaatcc tcaaaattat tatctactac ctgctattat tactctgtta gttttcattt
      541 attctttcct tataaaactt ctctatagct tccctttctt ctctcacctt attttcttct
      601 gtatatcctt cctgctctct aaaccctttc ttcatttctt tgaacaattt ccttccctgt
      661 ctcctattca aactgttttt gatatttacc atgtttaatc tgacttctcc gtcttcataa
      721 accgttaaga ttttagtcgt gtaatattcg tccaatttca tatctaagtg tccagaaata
      781 ttttcttttt tcacatattc tatcaagtcc tctaacaatt ttctgttatt tgacctatga
      841 gtttgtaaaa acacaataaa gtcggataat ttaaagcaaa cataggaaac aacaagactt
      901 gctaaaaaca ccgagttaac gaaaggaaag aaagataagt acactaaagg gtagtaatcc
      961 ccttctaatt ttagagtttc cttctgctta ccaataacaa gtaaaggtaa gactacacta
     1021 atcaaataaa ctgctattat tttataaatc ataaccactt acttttaatt ttgaataact
     1081 ctaattctca gcccaaacac attaccctct ttatcaaccc ttataagatc ttcttttata
     1141 gctttatgta tttctacacc aataccatca caaagtcttt gaataatata ttcttctggg
     1201 atatagagac tactgtcaaa actattgtca tat
//
LOCUS       DATGSI010000196         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_158001, whole genome shotgun sequence.
ACCESSION   DATGSI010000196 DATGSI010000000
VERSION     DATGSI010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158001"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..715)
                     /locus_tag="VK031_02995"
     CDS             complement(191..715)
                     /locus_tag="VK031_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HLS52912.1"
                     /translation="MKKKKITVSVLVKAAFLTAISIVLTRSLPLITKTLRIGLGEVPL
                     MLSGFLFGPVVGGMAGFVADMVGIIINPQGPPHIGFTISSMLWGIIPGLFVSNYEKLK
                     KEANPYTGLNITLVVSLCILIISIGLNTFWLSRLYGRAFIVLLPERLLMALVNIPIQS
                     LIITKLIKHLRKLS"
     regulatory      complement(777..868)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(882..1814)
                     /locus_tag="VK031_03000"
     CDS             complement(882..1814)
                     /locus_tag="VK031_03000"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HLS52913.1"
                     /translation="MKKLWERIKSYSIKNNNKRILKNYYSKEIRRGKTYRAYLVDKIL
                     LIFLIYFGLSIYLSLKTGYIFLSFYMVGMTLFFLIYLIKIYINRRDREKIKAINENLK
                     RKKLIRDFSNLTRNGFIKFVQDLLVAHFDTEIIETDGDIDFIGTIDGDNFGVKCIKYN
                     MDDKVPLRELNNFTRELKRLNISDGILITNSYFRDDVRDEARVIIYDFDNIVEMLKSI
                     GEYPSDKDMENYIIDRFLGRRNSVRQQVRQINKKKILQLYGIFVIFYTLSFVVRFSMY
                     YRIIAVLVFVFTTIVVGYKFSEYIRLKDRYPLNK"
     gene            complement(1789..2457)
                     /locus_tag="VK031_03005"
     CDS             complement(1789..2457)
                     /locus_tag="VK031_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HLS52914.1"
                     /translation="MRKFLIFLLVVSLSLTVLAISIEKNAYDMDYYLKSYEKNKISQL
                     TGKSKGQLAEISENLVSYIKGQGGEELLEPYFNEKEILHMVDVQNLFNLARRIKYIGL
                     VLSIGIVINFLKDRKYKLLGKALLFGPFVNYFIFLILGILAYIDFNKYFTYFHLIFFT
                     NDLWILDPNKDLMIQMLPENFFMGMAKNILLSFLLYLAIIQVIGCIILRRGRIINEKV
                     VGKD"
     gene            complement(2513..>2698)
                     /locus_tag="VK031_03010"
     CDS             complement(2513..>2698)
                     /locus_tag="VK031_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52915.1"
                     /translation="RRLLMEGKENFQHILERFQEADLEQKIEIYTTVRGLSVEQYKEL
                     LRYFPIKHLGKLEQAMG"
BASE COUNT          927 a          520 c          313 g          938 t
ORIGIN      
        1 tggatacaaa ctgccccaaa ttcatattca atagcttctt ggcataatct tttaacatct
       61 tcttttcttg catcagcctt taataaggtg tgatcaatca ttttattaat ttgctccata
      121 ctaaccctcc caagctattt aaattcaaat ctaatatcaa ttatatatat gaaaaaaata
      181 aaagcaacca ttatgagagt tttcttaaat gttttattaa cttagttata attaagcttt
      241 gaattggtat attaactaag gccataagca gcctttcagg taataaaact ataaaggctc
      301 ttccatataa tctggataac caaaaggtgt taaggccgat tgaaattatt aaaatgcata
      361 ggctaactac taaggtaata ttaagtccgg tataaggatt agcctccttt ttaagctttt
      421 cataattaga aacaaataag cctggtatta taccccatag catggagctt atggtaaatc
      481 caatatgagg tggaccttga gggttaatta taataccaac catgtcggct acaaatccag
      541 ccatgcctcc tactactggt ccaaataaaa aaccagacaa cattagaggc acttcgccta
      601 accctatcct tagggtcttt gtaatcaagg gtaaggatct ggttaaaact attgatatgg
      661 ctgttaaaaa ggcagcctta accagtacac ttacagtaat cttctttttc ttcacaaaaa
      721 aactcctttc ttgttagtga caaaatactg ccacaaagaa aagaggatct ttaatggcca
      781 gcggatgcga taaaaagacg cagatttctc tttcgtttaa aggcaacctc ccatccttta
      841 acacttaacg ctttttacct actctgtccc taacatttat attatttatt taaaggatat
      901 ctatccttta atctaatata ctcagaaaat ttatacccaa caactatggt ggtaaacaca
      961 aacaccaata cagcaattat tctatagtac atagaaaacc tcaccacaaa agataacgta
     1021 taaaatataa caaagattcc atataattgc aagatttttt tcttgtttat ctgccttacc
     1081 tgttgtctta cactatttct tctacctagg aacctatcaa taatataatt ttccatatcc
     1141 ttatcactag gatactcacc tatactctta agcatttcca ctatattatc aaaatcatat
     1201 attataactc tcgcttcatc tctaacatca tccctaaagt aagagttagt aattagaata
     1261 ccatcactta tgttaagcct tttaagctct ctggtaaagt tattaagctc ccttaagggt
     1321 accttatcat ccatattata cttaatacat ttcacaccaa aattatctcc atctatagta
     1381 cctataaagt ctatatcccc atctgtctca ataatttctg tatcaaaatg cgcaaccaac
     1441 aaatcctgaa caaacttaat aaagccattt ctagttaagt tggaaaagtc tctaataagc
     1501 tttttcctct ttaagttttc atttattgcc tttatctttt ccctatccct tctattaata
     1561 tatattttta taagatagat taagaagaat aaggtcatcc ctaccatata aaaagatagg
     1621 aagatgtaac ctgttttcaa gcttaaatat atacttaaac caaaatatat cagaaatatt
     1681 aaaagaatct tatcaactag ataggcccta taagtcttgc ccctccttat ttctttggaa
     1741 taatagtttt ttaaaatcct tttattattg ttttttatag aatagctttt aatcctttcc
     1801 cacaactttt tcattaatta tcctgcccct tcttaaaata atacagccta ttacctgtat
     1861 tattgccaaa taaagcagga aagataacaa aatattttta gccattccca taaagaagtt
     1921 ttcaggtaac atttgtatca tcaaatcctt atttggatct aatatccata aatcgttggt
     1981 aaaaaagatt aagtgaaaat aagtaaaata cttgttaaag tctatgtagg ctaaaatccc
     2041 taatataagg aagataaaat aattaacaaa aggtccaaat aaaagggctt tacccagtaa
     2101 tttatacttc ctgtctttta agaagtttat tacaattcct atagataaaa caaggccaat
     2161 atatttaatc cttctggcta aattaaataa attttgcaca tcaaccatat gtaaaatttc
     2221 cttttcattg aaatatggct ccagtaactc ctcccctccc tgtcccttta tataggaaac
     2281 tagattttca cttatttctg ccagctgccc tttagattta ccagttaatt gactaatttt
     2341 atttttttca taggatttta gataatagtc catatcataa gcgttttttt caatgctaat
     2401 tgctaaaaca gtcaaagaaa gggatacaac taataaaaaa attaaaaact ttctcatttt
     2461 ttcacctacc tattttaaaa ttatataaaa ataggatgaa gtagtctcca tcctatccca
     2521 tggcctgctc taatttcccc aaatgtttta tggggaaata tctgagtagc tccttatatt
     2581 gttctacact taatcctcta acggtggtat atatttctat tttttgttct aaatcagctt
     2641 cctgaaatct ttctagaata tgttggaaat tttcttttcc ctccataagc aacctcct
//
LOCUS       DATGSI010000197         1168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_14627, whole genome shotgun sequence.
ACCESSION   DATGSI010000197 DATGSI010000000
VERSION     DATGSI010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14627"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..803
                     /gene="rpsD"
                     /locus_tag="VK031_03015"
     CDS             204..803
                     /gene="rpsD"
                     /locus_tag="VK031_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HLS52916.1"
                     /translation="MAKMMGPRFKQSRRLGLNVCGHPKAMKRATRGTSRADKKLTEYG
                     IQLLEKQRLRAYYGVMEKQFIKYVQNAKKSPEQTGHALVKSLERRLDNLVYRIGFASS
                     IRQARQMVVHGHILVNGKKVDIPSYSVNIGDEISLRERSRKVELFAENYENSFLISYP
                     YIEKDKDNFSATLVRMPDREEIPIEIDDHLVVEFYSQLM"
     gene            complement(861..>1168)
                     /locus_tag="VK031_03020"
     CDS             complement(861..>1168)
                     /locus_tag="VK031_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52917.1"
                     /translation="TLLGLLGSIPITQIGLVIDILRPLLVWDNPQRAMKQNLNVLISL
                     GVGSLFVLLLGYGSWRLLNKLSINYILSLITIILLASSILLYYLLKRLLARQFTDLE"
BASE COUNT          433 a          181 c          209 g          345 t
ORIGIN      
        1 tatcatagtt gcctccttct tttatttata ttatatcagc ctttttaaca aaaatcacca
       61 aaagctatag atgtagtcgt cacttgcctt tttaaaaagg gagattattg ataacaatta
      121 tcatttacat tttcaaaaaa atatgttata ttattacaag gcttaaatta tcgcggcaaa
      181 taatttaaga aagaaggata attatggcta aaatgatggg acctaggttc aagcaatcta
      241 ggagattggg actcaatgta tgtggtcacc ctaaagctat gaaaagggca actaggggca
      301 catcaagggc tgataagaaa ctaactgaat atggaatcca attactggaa aaacaaagac
      361 taagagcata ttatggagtt atggaaaaac aatttataaa atatgtacaa aatgctaaaa
      421 aatcaccgga acaaactggc catgccttgg tcaagtcttt ggaaagaaga ttagataacc
      481 tggtttatag aataggtttt gcctcttcca ttagacaggc taggcaaatg gtagttcatg
      541 gtcatatttt agtaaatgga aaaaaagttg atataccctc ctactcagtg aatattggag
      601 atgagattag cctaagagaa agatccagga aagtagaatt atttgctgaa aactatgaaa
      661 atagtttctt aatatcctat ccatatattg aaaaggataa ggataatttc tcagccacct
      721 tggttagaat gcctgatagg gaagaaatcc caatagagat tgatgaccac ctagtagtag
      781 aattttactc acaattaatg taaaaaataa acagacagtt caatgataaa cttatttagt
      841 tagatcattg aactgttttt ttattcaagg tctgtaaatt gccttgctaa aagtcttttt
      901 aacagatagt aaagtaggat tgaactggcc aacaaaatta tagttataag ggaaagaata
      961 tagtttatac ttagcttatt caataagcgc caactaccat accccaaaag caagacaaat
     1021 aggctaccta cacctaagga tatgagtaca tttaagtttt gtttcatggc cctttgagga
     1081 ttatcccaca caagcaaagg tcttagtata tcaatcacta ggcctatctg cgttataggt
     1141 atactgccta acaagcccag aagggtta
//
LOCUS       DATGSI010000198         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_247959, whole genome shotgun sequence.
ACCESSION   DATGSI010000198 DATGSI010000000
VERSION     DATGSI010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247959"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2128)
                     /gene="ileS"
                     /locus_tag="VK031_03025"
     CDS             complement(<1..>2128)
                     /gene="ileS"
                     /locus_tag="VK031_03025"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HLS52918.1"
                     /translation="YLIDLDDPYITLENDYIESVWWILDKFFQEGLIYEGHKILPYCS
                     RCGTGLASHEVAQGYEEIKSETVVAKFKLKERDEYFLAWTTTPWTLPSNVCLTVNPDV
                     VYIKAKQEDEIYYVAKDLANKVLGDDYEVLEELTGRDLEYIEYEQLIPFLEAGKKAFY
                     VTLGDYVTTEDGTGIVHSAPAFGEDDYETGLKYDLPVLNPVSEEGKFIDTIWKDKFVM
                     DADLDIIQWLRENDKLYKKERVEHNYPHCWRCHTPLLYYAKPSWYIEMTKLKDQLIEN
                     NNKVNWYPAYVGERRFGNWLENLNDWAISRSRYWGTPFPVWRCDDCGHVDSVGSRKEL
                     VERAIEDIDMDIELHRPYVDKVHLKCDKCDGQMTREKDVIDVWFDSGAMPFAQHHYPF
                     ENKDIFFDELFPADFINEGIDQTRGWFYSLLAISTFVTGKSPFKNVLVNDLILDKEGK
                     KMSKSRGNTVDPFELFDKYGADVLRWYLVHVSPPWTPTRFDEDGLREVESKFFRSIRN
                     VYNFFSLYANTDDVDPREFFVEYEDRPEIDRWILSKYNSLLKKAKENMDSFEVTRVVR
                     DIQEFVIEDLSNWYIRRNRRRFWSTELDKDKKAVYNTSYEILVGISKIIAPFVPYTAE
                     ELYQNLVGGESVHLEYYPKANEELIDKDLEEKMDLVRDLVGLGRASRENVRIKVRQPL
                     NEVLIDGKYEEMISDLVPLIKEELNVK"
BASE COUNT          586 a          476 c          302 g          764 t
ORIGIN      
        1 ttttgacatt taattcttcc ttaatcagtg gaactaggtc tgatatcatt tcttcatact
       61 tgccatctat taacacttca tttaatggct gtctcacctt gattcttaca ttttccctgg
      121 aggccctacc aagtccaacc aggtcacgaa ccaggtccat tttctcttct aaatccttat
      181 ctattagttc ttcattggcc tttggataat actctaaatg gactgattct ccacctacca
      241 ggttttgata cagttcttct gcagtgtagg gtacaaaagg tgctattatc ttactgatcc
      301 ctactagaat ttcatagctt gtattatata ctgccttttt atccttatcc aattctgtag
      361 accagaatct tcttctattt cttctaatat accagttgga taaatcctct ataacaaatt
      421 cttgaatatc tcttactacc ctggttactt cgaagctatc catattttct ttagcctttt
      481 ttagtagtga gttatatttt gataatatcc atctatcaat ctctggccta tcttcatatt
      541 ctacaaagaa ctcccttggg tccacatcgt ctgtattggc atatagggag aagaaattat
      601 atacatttct tatggatcta aagaatttag attcaacttc ccttaagcca tcttcatcaa
      661 atctagttgg agtccagggc ggtgatacat gtactaggta ccatcttaaa acatcggcac
      721 catatttatc aaacagttca aagggatcca ccgtgttccc tctagactta gacattttct
      781 tgccttcttt gtccaggatt aaatcattta ccaatacatt tttaaaaggc gatttacctg
      841 taacaaaggt ggatatggcc agcaatgagt agaaccagcc tctagtttga tctattcctt
      901 cattgataaa gtctgctggg aataactcat caaagaatat atccttattt tcaaagggat
      961 agtgatgctg ggcaaagggc atagctccac tatcgaacca tacatcaatt acatcctttt
     1021 ccctagtcat ttgcccatcg catttatcac attttagatg aaccttatct acataaggcc
     1081 tatgcagctc aatatccata tctatatctt ctatggctct ttctaccaat tcttttctag
     1141 aaccaacact atctacatga ccacagtcat cacatctcca aacaggaaag ggagttcccc
     1201 aatatctact tcttgagatg gcccaatcat ttagattttc taaccagtta ccaaaccttc
     1261 tttcacctac ataggctggg taccagttta ccttgttatt gttttctatc aattgatctt
     1321 tcagcttggt catttctata taccaggatg gcttagcata gtataataag ggtgtatggc
     1381 atctccagca atgtgggtaa ttatgctcta ccctttcttt tttataaagc ttatcatttt
     1441 cccttaacca ctggataata tctaaatctg catccattac aaatttatct ttccagatgg
     1501 tgtctataaa ttttccttct tcagatactg ggtttaaaac aggtaaatca tattttagtc
     1561 cagtctcata gtcatcttca ccaaaggcag gagctgaatg aactattcct gttccatctt
     1621 ctgtggttac ataatctccc aatgttacat aaaatgcctt tttacctgct tctaaaaatg
     1681 ggattagttg ctcatattct atatactcca gatcccttcc tgttagttct tctaagacct
     1741 cataatcatc gcctaatacc ttatttgcca agtcttttgc cacataataa atttcatctt
     1801 cctgctttgc ttttatataa accacatctg gatttactgt taagcacacg ttggaaggca
     1861 gggtccatgg tgtagtggtc catgctaaaa agtattcgtc cctttccttt agcttaaatt
     1921 tagctactac tgtttctgat tttatctcct catagccttg agctacctca tgggaggcca
     1981 gccctgttcc acatcgtgaa caatatggta atattttatg gccttcataa attaaccctt
     2041 cttggaaaaa cttatctaat atccaccata ctgattcaat atagtcattt tccagtgtta
     2101 tatatggatc atctaaatct attagata
//
LOCUS       DATGSI010000199         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_534921, whole genome shotgun sequence.
ACCESSION   DATGSI010000199 DATGSI010000000
VERSION     DATGSI010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534921"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /gene="tyrS"
                     /locus_tag="VK031_03030"
     CDS             complement(<1..537)
                     /gene="tyrS"
                     /locus_tag="VK031_03030"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HLS52919.1"
                     /translation="MKNVFDLLSERGYIDQVTFEDELRNLLATEPVTFYIGFDATADS
                     LTLGHFVQIMVMMHMQKAGHRPIALLGGGTTMIGDPSGKAEMRQLMDKKTIDYNAGKF
                     KEQLSRFLDFGQDGALMLNNADWLLDLNFLEFMREVGVHFSVNRMLTLDSYKNRLEKG
                     LTFFEFSYLLMQSYDFLHL"
     gene            complement(851..1219)
                     /locus_tag="VK031_03035"
     CDS             complement(851..1219)
                     /locus_tag="VK031_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HLS52920.1"
                     /translation="MGDFYQSGDWKGEKHVPVIHAPEKVKAGEPVEVKVLIGEEIPHP
                     NTLEHYISWIKLYFHAEGAKFPVEIGTYNFSAHGEGDLFTEPHVVTRFTAEKSGTLLA
                     TSYCNIHGLWENSVEVKVEG"
     gene            1359..2504
                     /locus_tag="VK031_03040"
     CDS             1359..2504
                     /locus_tag="VK031_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HLS52921.1"
                     /translation="MSLIDMIATTSFGLEAVARRELEDLGYEDIRVENGRVNFSGTEK
                     DIARTNLWLRTADRILVKLGEFKALSFEELFEGTKALAWEEWIPKDGNFIVQGRSVNS
                     KLYSISDCQSIVEKAIVERLKTKYPVDWFDKTGPRYKVEVSLLKDIATLTIDTSGEGL
                     HKRGYRERAGDAPIKETLAAAMILLSYWNKDRTLLDPFCGSGTIPIEAAMIGRKIAPG
                     LDRSFDAEKWPRIKEKYWQDARTEAFAKIDHKSKLDILACDIDRRSILRARDNAANLG
                     LEEDIAFFMKDMRQVDIQDEYGVIITNPPYGERIGEKEEIHQLYKDFAKKYGKLDTWS
                     IYVVTSEKKFENLYGRRADRRRKLYNGRIEVNYYQYYGPRPPRKGGK"
     gene            2506..>2860
                     /locus_tag="VK031_03045"
     CDS             2506..>2860
                     /locus_tag="VK031_03045"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HLS52922.1"
                     /translation="MTIYTKTGDSGDTSLFDNVRVSKDDIRVESYGTVDELVSWLGLA
                     KNYVEDRDMYKWLEEIQHKLFTVASILATEDPKNIQHYIKEADINNLEKIVDFYMEKF
                     ESKEGFIIPGSGKGSA"
BASE COUNT          965 a          491 c          578 g          826 t
ORIGIN      
        1 caggtgtaaa aaatcgtaag actgcattaa aagataacta aactcaaaga aggtaagtcc
       61 cttttctagc ctgtttttat aactatccag ggtaagcatt ctatttacag aaaaatgaac
      121 tcccacttct ctcataaatt ctaagaaatt taaatccaaa agccagtcag cattgttaag
      181 cattagggcc ccgtcctggc caaagtctag aaatctactt aattgctctt taaacttgcc
      241 agcattgtag tcgatggttt ttttatccat caattgtctc atttcagctt taccagatgg
      301 atctccaatc atggtggttc ctccacctag cagggcaatt ggtctatgac ctgccttttg
      361 catgtgcatc ataaccatga tttggacaaa gtgacctaag gttaaactat ctgcggtagc
      421 atcaaagcca atataaaagg ttactggttc agtggctagt aaatttctta attcatcttc
      481 aaaggttacc tggtctatat aacccctttc agataaaaga tcaaatacat ttttcacatt
      541 atcacctcaa taatcttttg taattagaat aaaaaagggt atcttcttcg caaggacgag
      601 aagatacccg tggtaccacc ttaattcgcc agtatggcct caaaccctgt aacgtagggg
      661 gacgcaatag tttcctattg aaactccaga tccgtaattc gctcaacttt gaagttacag
      721 gcctctcacc aatgacctat tctctgtatt taactaagtg gagctactta atccttcatt
      781 gttaactatt aaatttatat gtatattatt atattaccca gttcttgtca agggtaaaag
      841 agggctaaaa ttagccctcc accttcactt ctacactatt ttcccataaa ccgtggatat
      901 tgcagtagct ggttgccaaa agagtacctg atttttcagc agtaaatcta gtaaccacat
      961 gaggctcagt aaacaaatct ccttctccat gggctgagaa gttataagta ccaatttcta
     1021 ccgggaattt agctccttct gcatggaagt aaagcttaat ccagctaata taatgttcta
     1081 atgtgttagg atgtggtatt tcctcaccta ttaaaacttt aacctctaca ggctctccag
     1141 ccttgacttt ttctggagca tggattacag gtacatgttt ttcacctttc caatctccac
     1201 tttggtaaaa atcccccatt tttttcaatg ttaactcccc tttctattat aaatattcac
     1261 aatatacata tacccagcct gattttaaat acccaaatat attactatat tccacattat
     1321 aagatataat ataagtaagc atagaaggag agatgataat gagtctgatt gatatgattg
     1381 caactactag ttttggtctg gaagctgttg caagaaggga actagaggat ttaggatatg
     1441 aagatataag agtagaaaat ggaagggtca attttagcgg gacagaaaaa gacatagcca
     1501 ggaccaattt atggcttagg actgcagata gaattttagt aaaattaggt gaatttaagg
     1561 ccttaagttt tgaggaattg tttgaaggga ctaaggcctt agcctgggag gaatggatac
     1621 ctaaggatgg taattttata gtacagggta gaagtgtaaa ctctaaatta tatagtattt
     1681 cagattgcca aagtatagtg gaaaaggcca tagttgaaag attaaaaaca aaatatcctg
     1741 tagactggtt tgataaaaca gggcctaggt ataaggtgga ggtgtccctt ttaaaagata
     1801 tagctacctt aacaatagat acttcaggag aaggactaca caaaaggggc tatagagaga
     1861 gggctggaga tgctcccatt aaagagactt tggctgctgc catgatacta ttaagctatt
     1921 ggaataagga taggaccctt ttggatccct tctgtgggtc aggaactatt cccatagaag
     1981 ccgccatgat aggaagaaag atagctcctg gtcttgatag aagctttgac gcagaaaaat
     2041 ggcctagaat aaaagaaaag tattggcagg atgcaagaac agaagccttt gccaaaatcg
     2101 atcataaaag caagctagat attttggcct gtgatataga tagaagatca attttaaggg
     2161 ccagagataa tgcggccaac ttggggctgg aagaagatat agccttcttt atgaaagata
     2221 tgagacaggt ggatatacag gatgagtatg gggttattat taccaaccca ccctatggtg
     2281 aaagaatagg tgaaaaagaa gagatccacc aattatataa ggattttgca aaaaaatatg
     2341 gaaaattgga tacctggtct atatatgtgg ttacttctga aaaaaaattc gaaaaccttt
     2401 atggaagaag ggcagacagg agaagaaagt tatataatgg aaggattgag gtgaattatt
     2461 accagtatta tgggcctagg ccacctagaa aaggaggaaa ataggatgac aatctataca
     2521 aaaactggtg acagcggtga tacttcctta tttgacaacg ttagggtatc aaaagatgat
     2581 attagggttg aaagttatgg caccgtagat gaattggttt cctggctagg attagccaaa
     2641 aactatgtgg aagataggga tatgtataag tggttggaag aaatccagca caaactcttt
     2701 actgttgcat caattttagc aacagaagac cctaagaata tacagcatta tatcaaggaa
     2761 gctgacataa acaatttaga gaaaatagta gatttctata tggaaaagtt tgaaagcaaa
     2821 gaaggcttta taattcctgg ctcaggcaag ggatcagcct
//
LOCUS       DATGSI010000200         1002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_110943, whole genome shotgun sequence.
ACCESSION   DATGSI010000200 DATGSI010000000
VERSION     DATGSI010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110943"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /gene="nagB"
                     /locus_tag="VK031_03050"
     CDS             complement(<1..556)
                     /gene="nagB"
                     /locus_tag="VK031_03050"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HLS52923.1"
                     /translation="MEVIVKKDYESLSKQVAEILAEEIRKKPDIKLGLATGSTPVGTY
                     EELIKLHKQKGLDFSKVKTFNLDEYIGLSGDHPNSYRYFMNDILFDHINIKKENTFIP
                     NGKARDPKKECKAYDKLIEEAGGIDIQILGIGTNGHIAFNEPAEELSAGTSVVDLTEN
                     TIKDNSRFFDSIDEVPKTAITMGIV"
     gene            complement(561..>1002)
                     /locus_tag="VK031_03055"
     CDS             complement(561..>1002)
                     /locus_tag="VK031_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLS52924.1"
                     /translation="DYWWQSLKQINDLGLETLFFIQNNSYRYKDTVIYGTRGWISRDS
                     EDFNENDEKVFRRELLRLEMSLESQKQAKKKIVMIHHPPFNMDLSPNEFVTIMKDYGV
                     DICVYGHLHSDGHRFAVEGEIEGILFHCISCDYIDFKPKIILGE"
BASE COUNT          289 a          217 c          130 g          366 t
ORIGIN      
        1 tgactatacc catggttatg gcagttttag gtacctcatc tatagaatca aaaaatcttg
       61 agttatcctt gatcgtattt tccgtcagat ccacaacaga agtacctgct gataattctt
      121 cagctggttc attaaaggca atatggccat ttgtaccaat tcctaagatt tgtatatcaa
      181 tgcctccagc ctcctcaatt aacttatcat aggccttaca ctcctttttt ggatcccttg
      241 ccttaccatt tggtataaag gtgttttctt ttttaatatt aatatgatca aataaaatat
      301 cattcataaa gtatctataa ctatttggat gatctccact tagccctata tattcatcca
      361 ggttaaaggt tttaacctta gaaaaatcta accctttttg cttatggagc ttgattaatt
      421 cttcatatgt tcctactggc gtgctacctg tggccaagcc taatttaata tctggctttt
      481 ttcttatttc ctcagccaag atttcagcca cctgcttgct taaactttca tagtccttct
      541 taacaattac ttccacacta tcactctcct aaaataattt tcggtttgaa gtctatataa
      601 tcacatgaga tacagtggaa taaaatgcct tctatttccc cttccactgc aaatctatgt
      661 ccatctgaat gcaagtgtcc atacacacat atatctaccc cataatcttt cataatagtc
      721 acaaattcat tgggagatag gtccatatta aagggaggat gatggatcat aacaattttt
      781 ttcttagcct gtttttgact ttcaagtgac atctccagcc ttaaaagttc ccttctaaaa
      841 actttttcgt cattttcatt aaaatcttcg ctatctctag aaatccagcc tctagttccg
      901 tatattacgg tgtccttata cctgtatgaa ttattttgaa taaaaaataa ggtttccaac
      961 cccagatcat ttatttgttt taaactttgc caccaataat cg
//
LOCUS       DATGSI010000201         1011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_535290, whole genome shotgun sequence.
ACCESSION   DATGSI010000201 DATGSI010000000
VERSION     DATGSI010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535290"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="VK031_03060"
     CDS             complement(<1..124)
                     /locus_tag="VK031_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLS52925.1"
                     /translation="MSKEVFERTKPHVNIGTIGHVDHGKTTLTAAITMVLNKRLG"
     gene            complement(194..269)
                     /locus_tag="VK031_03065"
     tRNA            complement(194..269)
                     /locus_tag="VK031_03065"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Thr,seq:ggt)
     gene            complement(326..913)
                     /gene="sigH"
                     /locus_tag="VK031_03070"
     CDS             complement(326..913)
                     /gene="sigH"
                     /locus_tag="VK031_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HLS52926.1"
                     /translation="MEEEDIVDAARMGNSLAQEYLINKYKGFVHGKARTYFLIGADRD
                     DLVQEGMIGLYKAIRDYDRDKLTSFKCFAEICITRQIITAIKTATRQKHIPLNSYISL
                     NKPMYDEDSERTLLDIINSNKGNDPMDVFIGRENMNNMEARMSEVLSKLEWDVFQRYI
                     SGKSYQEIAKSLGRNVKSVDNALQRVKRKMEKVLL"
BASE COUNT          281 a          219 c          137 g          374 t
ORIGIN      
        1 cacctaatct cttgtttaat accatggtga ttgctgctgt taatgtggtt ttaccatggt
       61 ctacgtgtcc tattgtccct atatttacgt ggggtttggt tctttcaaat acttcttttg
      121 acatttgatt ctcctcctta atcttctcag tttcaaggaa acttatctgt attttcgcaa
      181 taaaatatat taatggagcc cacgaccgga tttgaaccgg tgacctcttg cttaccatgc
      241 aagtgctcta cctcctgagc tacgtgggcc agctgttaaa cagtttacta cttaatatta
      301 accatgtcaa tagtaaatta aaaaattata aaaggacttt ctccatcttc ctttttaccc
      361 tctgcaaggc attatcaacg cttttcacat ttctacctaa agacttagct atctcctggt
      421 aagatttacc tgatatatat ctttggaata catcccactc cagcttacta agcacttcag
      481 acatcctagc ttccatatta ttcatatttt ccctacctat gaacacatcc ataggatcat
      541 ttcccttatt actatttatt atatctaata aagtcctttc agaatcctca tcatacatag
      601 gtttgtttaa tgatatatag gaattaaggg gtatatgctt ttgtctggta gctgttttaa
      661 tggccgtaat tatttgtctc gttatgcaga tttctgcaaa acatttaaag gaggttagct
      721 tgtccctatc atagtccctt atggccttat ataagcctat cattccttct tgaactaaat
      781 cgtccctatc agcccctatt aaaaaatagg tccttgcttt cccatgtaca aaacccttgt
      841 atttgtttat taaatattct tgagccagac tattacccat tcgtgcagca tcaactatat
      901 cttcttcctc catatcttca taaatcaagg tgcaagagtt atcgtatagt ttgaatacca
      961 caaaacatct ctccttattg aaagcaatta atttaattat aaataatttt g
//
LOCUS       DATGSI010000202         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_535313, whole genome shotgun sequence.
ACCESSION   DATGSI010000202 DATGSI010000000
VERSION     DATGSI010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535313"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="VK031_03075"
     CDS             complement(<1..124)
                     /locus_tag="VK031_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLS52927.1"
                     /translation="MSKEVFERTKPHVNIGTIGHVDHGKTTLTAAITMVLNKRLG"
     gene            complement(148..2217)
                     /gene="fusA"
                     /locus_tag="VK031_03080"
     CDS             complement(148..2217)
                     /gene="fusA"
                     /locus_tag="VK031_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HLS52928.1"
                     /translation="MPRQVSLENTRNIGIMAHIDAGKTTTTERILFYTGKIHKVGETH
                     EGAAEMDWMEQEQERGITITSAATTCMWRDHRVNVIDTPGHVDFTVEVERSLRVLDGA
                     VAVFCAKGGVEPQSETVWRQADKYGVPRLAFVNKMDIVGADFYRAVGMIEDRLRANPV
                     PIQLPIGKEDEFIGVVDLVNMNARIYKDDLGEDIEIAPIPEDMVDIADEYREKLVEAV
                     AENDEELMMKYLEGEELTTEEIVQGIRNATIQVEIIPVLCGSSYKNKGVQPLLDAIID
                     YLPSPVDIPPVKGIDASTEEESERKSSDDEPFSALAFKIVTDPYVGKLAYFRVYSGTL
                     KSGSYVYNSSKGKRERIGRILLMHANKREEVDRVYAGDIAAAVGLRDTGTGDTLSSED
                     EPIILETMEFPEPVIHVAIEPKTKASQDKLSEALTKLAEEDPTFKTYTDEETGQTIIA
                     GMGELHLEIIVDRLLREFKVEANVGNPQVAYKESITGTAEADTKYARQSGGRGQYGHV
                     KIKIEPQEPGEGYEFVNKIVGGVVPREYIGAVDAGIQEALQSGILGGYPVLDVKVTLF
                     DGSYHEVDSSEMAFKIAGSMAVKEALSKANPVLLEPMMKVEVIIPEEYMGDVMGDINS
                     RRGRIEGMEMRSGAQVVNAYVPLSEMFGYATDLRSNTQGRGVYSMQFDHYEPVPANIA
                     EEILGKD"
     gene            complement(2272..>2559)
                     /gene="rpsG"
                     /locus_tag="VK031_03085"
     CDS             complement(2272..>2559)
                     /gene="rpsG"
                     /locus_tag="VK031_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HLS52929.1"
                     /translation="FHRALNNIMPVIEVKARRIGGATYQVPMEVRPERRETLGLRWLV
                     TYSRNRGEKTMIERLAKEIIDASNGVGGSVKRREDVHKMAEANKAFAHYRW"
BASE COUNT          639 a          632 c          384 g          904 t
ORIGIN      
        1 cacctaatct cttgtttaat accatggtga ttgctgctgt taatgtggtt ttaccatggt
       61 ctacgtgtcc tattgtccct atatttacgt ggggtttggt tctttcaaat acttcttttg
      121 acatttgatt ctcctcctta acaataacta gtctttacct aaaatttctt cagctatatt
      181 ggctggcact ggctcataat ggtcaaactg catggaatat actcctctac cttgagtgtt
      241 agaccttaag tctgtggcat atccaaacat ctcagataaa ggtacgtaag cattgactac
      301 ttgagcacca cttctcattt ccattccttc tattcttcct cttcgagagt taatatctcc
      361 cataacatca cccatgtatt cttctggaat aattacttcc accttcatca taggttctag
      421 aaggactgga ttagccttgc ttaaggcttc ctttactgcc atggaaccgg caatcttaaa
      481 ggccatctct gatgagtcaa cttcgtggta ggaaccatca aataaggtta cctttacatc
      541 tagtactgga tacccaccaa gaattcctga ttgtagggct tcttgaattc cagcatctac
      601 agcacctata tattctcttg gtactacacc accaacaatc ttgttgacaa attcgtagcc
      661 ttcacctggc tcctgtggct caattttgat cttaacatga ccatattgtc cacgcccacc
      721 agattgtctc gcatatttag tatctgcttc agcagtacct gtaattgatt ctttataagc
      781 aacctgtggg ttaccaacat tagcttcaac tttaaattct cttaagagtc tgtctactat
      841 aatctctaga tgtaattctc ccattccagc gattattgtt tgaccagtct cttcgtcagt
      901 atatgttttg aaggttggat cctcttcagc caactttgtt aaagcctcac ttagcttatc
      961 ctgactagcc ttagtcttag gctctatagc tacgtgaatt actggctcag ggaattccat
     1021 agtctctagg attattggtt catcttcaga agatagggta tctcctgttc ctgtatccct
     1081 taacccaact gctgcagcta tatctcctgc ataaactcga tccacttctt ccctcttatt
     1141 ggcatgcatc agtaatattc taccaattct ctctcttttc cctttagaag aattatagac
     1201 ataactacct gattttaaag ttcctgaata aactctgaaa tatgcaagtt tacctacata
     1261 aggatctgtc actattttga aagctaaggc tgaaaacggt tcatcatcac tagactttct
     1321 ttctgactct tcttctgtag atgcatctat cccctttaca ggtggaatat ctaccggtga
     1381 tggtaaataa tcaattatag catccaatag tggttgaacg cctttgtttt tataggaaga
     1441 accacatagt acaggaatta tttctacttg aatagtagca tttctgattc cttgaactat
     1501 ttcctcagtt gttaattcct ccccttccaa atacttcatc attaattctt catcattttc
     1561 agctacagcc tctactagct tttccctgta ttcatcagct atatctacca tatcttcagg
     1621 gattggggct atttcaatat cttcccctaa atcatccttg tatattctgg cattcatatt
     1681 aactaaatca actacaccaa taaactcatc ttctttgcct attggtaatt gtattggaac
     1741 cggattggcc cttagcctat cctcaatcat tccaactgct ctataaaagt ctgcaccaac
     1801 aatatccatc ttatttacaa aagccagacg gggcactcca tacttatctg cctgtctcca
     1861 tactgtctca gactggggct ctaccccacc ctttgcacag aatacagcaa ctgcaccgtc
     1921 tagtactcta agggaccttt caacttctac tgtgaaatcc acatggccag gcgtatcaat
     1981 aacattaacc cgatgatctc gccacataca ggtggtagca gctgaagtaa ttgttatacc
     2041 tctttcttgt tcctgctcca tccagtccat ttcggcagca ccctcatggg tttcgcctac
     2101 cttgtgtatt ttccccgtgt agaatagtat tctctctgtg gtggttgtct ttcctgcatc
     2161 aatatgagcc attatgccta tatttcttgt attttctaat gaaacttgtc ttggcacaat
     2221 ttttcctcct tctaatttct taagtccccc tccacacatt taaaaagatt attaccatct
     2281 atagtgtgcg aaagccttgt tagcttcggc catcttgtgt acatcttctc ttctttttac
     2341 acttccacct actccatttg aggcgtctat aatctctttt gctaatcttt caatcatagt
     2401 tttctcgcct ctgtttctag aataggttac tagccatctt aagcctaaag tttctcttct
     2461 ttcaggtctt acttccattg gtacctgata tgttgcccca cctatccttc tagctttaac
     2521 ttctataaca ggcataatat tgtttagggc cctgtggaa
//
LOCUS       DATGSI010000203         1056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_296426, whole genome shotgun sequence.
ACCESSION   DATGSI010000203 DATGSI010000000
VERSION     DATGSI010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296426"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VK031_03090"
     CDS             complement(<1..1043)
                     /locus_tag="VK031_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLS52930.1"
                     /translation="MGFLDPPKETARPAIKALHSRGVDVKILTGDNDAVTKKIAADLD
                     IPTENVVLGSDIENISDEELYKLASRTSILAKLSPVQKERVISILQSNNHVVGFLGDG
                     INDAQGLKQADVGISVDTAVDIAKESADIIMLEKDLNVLEEGVVEGRRVFGNIIKYIK
                     MTASSNFGNVFSVLVASAFLPFLPMLPIQLLVQNLLYSISQISIPWDNMDEEYIKQPQ
                     QWNAGEIGKFMIYIGPISSIFDIVTFWVMWSVFRANTRELMPLFQSGWFVVGLLTQTL
                     IVHMIRTRKIPFIQSRASTPVMVMTFAIMAVGLVIPFTPFGSYIGLVPLPANYFIWLF
                     GILLSYSLLTEVV"
BASE COUNT          344 a          225 c          176 g          311 t
ORIGIN      
        1 ttaactactt cagttaatag ggaataagac agcaggattc caaataacca gataaagtag
       61 ttggctggta aaggcactaa ccctatataa ctaccaaagg gagtaaaggg tattacaagt
      121 cctacagcca taatagcaaa ggtcatgacc ataacaggag tagatgccct actctgaata
      181 aatggtattt ttctagtcct aatcatatga actattaagg tctgggttag caggccaact
      241 acaaaccaac ctgactggaa taaaggcatt aattctctag tattggctct aaacacagac
      301 cacatgaccc aaaaggttac tatatcaaat atagaactta taggccctat ataaatcata
      361 aactttccaa tctcaccggc attccattgt tgaggctgtt tgatatattc ctcatccatg
      421 ttgtcccaag gtatagaaat ttgtgaaatg ctatatagca aattctgcac cagtaactgg
      481 ataggtagca taggcaggaa gggcaaaaag gcactggcaa ctaaaacact aaatacatta
      541 ccaaaattag agctggcagt catcttaata tatttaataa tattaccaaa gactctccta
      601 ccttcaacta caccttcttc taaaacattt aaatccttct ccaacatgat tatatctgcc
      661 gattcttttg ctatatcaac tgctgtatcc acagagattc ccacatcagc ctgcttaagg
      721 ccttgtgcat cattaatacc atcaccaaga aagcctacca catggttatt ggactggagg
      781 atggatatta ccctttcttt ttgaactggt gatagtttgg ctagaatact ggttcttgag
      841 gccagcttat acaattcttc atctgaaata ttctctatat ccgatcctag tacaacattt
      901 tcggtaggaa tgtctaaatc tgcagcaatt tttttagtaa ctgcatcatt atctccagtt
      961 aatatcttta catctactcc ccgactatga agggccttaa tggcaggcct ggcagtttcc
     1021 ttaggtggat ctaaaaagcc catatatcca attagg
//
LOCUS       DATGSI010000204         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_438957, whole genome shotgun sequence.
ACCESSION   DATGSI010000204 DATGSI010000000
VERSION     DATGSI010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438957"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..134
                     /locus_tag="VK031_03095"
     CDS             3..134
                     /locus_tag="VK031_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HLS52931.1"
                     /translation="MTDFKDIMKYGVMTTPALVVDEEVVSSGKVLKSKEIVKLLKKV"
     gene            253..1089
                     /locus_tag="VK031_03100"
     CDS             253..1089
                     /locus_tag="VK031_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HLS52932.1"
                     /translation="MIRLGEVQELEIKRFASVGAFLNAEEELERDVLLPKSQVPENAK
                     VGDKVKVFIYNDSRDRIIATTTMPKASLGQLGYLTVVDATRIGYFLDWGLDKDLFLPF
                     GETVGTVEKGKSYLVGVYIDKSNRLCATMKIRDMLSTDSPFKENDLVVGTIYSINRDI
                     GAFVAVEDKYDGLIPKKELLGVYELGQRVEVRISRVLDDGKLDLSLRDRAYIQMETDA
                     QTIMERLKENKGILNLNDNSPAQLIREELEMSKSGFKRAVGRLLREEMIEFTKNGIRL
                     KE"
     gene            1210..>2248
                     /locus_tag="VK031_03105"
     CDS             1210..>2248
                     /locus_tag="VK031_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52933.1"
                     /translation="MKRKRKGSIMALSIILFAILSGLGTVGLGMSINENKRAIRHQAK
                     TQAYYIARSGAEVVEAAILNLESEEEKEGLLKNLPKTINVKGLEFGFDGLGHISLEGN
                     RDKLIIVSEGKVENVIETVEKIINVEINKYEEDIISNIDTTIFSNNNMIFKNNTQNNV
                     NGAIGTNSIISGSLKFDSGFNDKITIKIPVGADSSSIIDKPSYDRSIPKVEYIEKRIY
                     SSPILPAYPENLPERGIIETSYHNDNPIISRNGYYDRLTIKSNRSVTIDTRKGDVDLR
                     IREFDVQRGDIMVIGEGKVNIFIEEFKNFNGNINGEGNSKKFRLYYNGSEPIIIAGSD
                     KINGSLYIEQAD"
BASE COUNT          865 a          260 c          489 g          634 t
ORIGIN      
        1 aggtgacaga ctttaaggat attatgaagt atggagtaat gaccacacct gccctagttg
       61 tggatgaaga agtagtttca tcaggcaagg ttttaaaatc caaggagata gtaaaactac
      121 taaaaaaagt ttaagatata attttctccc aatggtaaat aatatagcca tataggcaaa
      181 attggcatcc ttattgtaaa tccctatttt ctttgataga atgtatctag caaatataaa
      241 agaaggtgta ttatgataag attaggtgaa gttcaggagt tagaaataaa gaggtttgca
      301 tcagtagggg cttttttaaa tgctgaggag gagcttgaga gagatgtatt acttccaaag
      361 agtcaggtac ctgaaaatgc aaaggtagga gataaggtta aggtatttat atataatgac
      421 tcaagagata ggattattgc cactactact atgcctaagg caagcttagg ccaattagga
      481 tatttaacag ttgtggatgc aactaggata ggttattttc ttgattgggg cttagacaaa
      541 gatttatttt taccctttgg agaaacagtt ggaactgttg agaaggggaa atcttatctg
      601 gtgggggtct atatagataa atctaatagg ctatgtgcaa ctatgaaaat aagagacatg
      661 ctaagtacag attcaccatt taaggaaaat gatctggtgg taggtaccat ctatagtata
      721 aatagagata taggagcctt tgtggcagta gaggataaat atgatggatt gattccaaag
      781 aaagaacttt taggggtcta tgagttagga caaagggttg aagttaggat aagcagggtc
      841 ttagatgatg gaaaattaga cttaagtcta agggataggg cctatatcca gatggaaaca
      901 gatgctcaaa ccataatgga gagattaaag gaaaacaagg gtatacttaa tttaaatgat
      961 aatagtccgg cccagcttat cagggaagaa ttggaaatga gcaagtcagg ttttaaaagg
     1021 gctgtaggta gacttttaag ggaagagatg attgaattta ctaagaatgg aataagactg
     1081 aaggaataaa tataaaagcc gaagtttaaa cttcggcttt tgtaattccc tttgcaaaag
     1141 ctgtattatc gtatagaatg taggtaaaga agatggaaat gggtaataga aaataaaaag
     1201 gagatttaaa tgaaaagaaa acgaaagggc tccataatgg ccttatccat aatattattt
     1261 gccatattaa gtggtttggg aacagtaggg ctaggtatgt ctattaatga aaataagagg
     1321 gctataagac atcaagctaa aacccaagct tattatatag ccagatcagg ggcagaagta
     1381 gttgaagcgg ccatattaaa tctagaatca gaagaggaaa aagaagggct ccttaaaaac
     1441 ttgccaaaaa ctattaatgt aaagggtctg gaatttggtt ttgatggttt aggacatata
     1501 agtttagaag gaaatagaga taagctaatt attgtttcag aaggtaaggt agaaaatgta
     1561 attgaaacag ttgaaaagat aatcaatgtt gagataaata aatatgagga agatataatt
     1621 tccaatattg atacaactat atttagtaac aataatatga tatttaaaaa taacacacaa
     1681 aacaatgtga atggagctat aggaactaat tcaatcattt ccggctcact aaaattcgat
     1741 tctggattca atgataaaat tacaataaaa atcccagtag gagcagattc tagtagtatc
     1801 atagataagc catcatatga tcgtagcata cccaaagtag agtatattga aaaacgaata
     1861 tattcttctc ccatacttcc agcttaccca gaaaatttac ctgaaagagg aattatagaa
     1921 acaagctatc ataatgacaa tccaataatt agtagaaatg gatattatga tagattaact
     1981 ataaaatcaa accgttctgt aacaattgat acaagaaagg gagatgtaga tttaaggata
     2041 agggaatttg atgttcaacg tggagatatt atggtgattg gagagggtaa agtaaatata
     2101 tttatagaag aatttaagaa ctttaatggt aatataaatg gtgaagggaa tagtaaaaaa
     2161 tttcgtcttt actacaatgg ttcagagcca ataataattg ctggtagtga caaaatcaac
     2221 gggtctctgt atatagaaca agctgact
//
LOCUS       DATGSI010000205         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_439002, whole genome shotgun sequence.
ACCESSION   DATGSI010000205 DATGSI010000000
VERSION     DATGSI010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439002"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VK031_03110"
     CDS             complement(<1..929)
                     /locus_tag="VK031_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HLS52934.1"
                     /translation="MEKKRMGLATRVLIGLALGVVVGIIVYGFPDGTLKDAFLINGLF
                     QLVGQIFLRSIMMLVVPLVFISLVNGAASMGDIKKLGRVGVKTISFYLVTTAIAIVIS
                     LVIAFLINPGVGLDMGAIDAVDVSESETTPLVQILYEMVPTNPVASLSEGSMLQIIVF
                     AILSGIALSIIGEKGTKVLELFEQLNELVMELVRMVMVLAPVGVFSLVARTFATVGVA
                     ALVPILKVVLGVYIGLVIHATLVYGGLLKAFTGLSIFKFYKKFYPAMAIAFSTASSNA
                     ALPVSMDLAENQIGVSKEIAAFALPLGATINMDG"
     gene            complement(1059..>2265)
                     /gene="gatB"
                     /locus_tag="VK031_03115"
     CDS             complement(1059..>2265)
                     /gene="gatB"
                     /locus_tag="VK031_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HLS52935.1"
                     /translation="TRIGKQDRKHYFYPDLPNSYQISQDDLPLCKGGYLDIEVGGRTK
                     RIGITRIHIEADAGKLIHKDGVGSLVDYNRAGVPLIEIVSEPDLRSAEETRAFLQKLR
                     AIMLYLNISDCKMNEGSFRCDVNLSVREKGHKEYGTRTEMKNLNSFQSIMRSIEYEAQ
                     RQIQEIKEGREIIQETRRFDQNTGRTFSMRSKEDSQDYRYMPDPDLMPIVIDEERVKK
                     LEASLPELPDVRKKTYMEKYGLNSYDAEQLISSKDVANYFEEAAKSSQDAKILANLMI
                     TEVFRITDLDDFKVPFEPKDLAELVNLLAEGRINTSTSKKVLDEMVKSKKSPAIIVKE
                     QDLEQINDRQTLLPIVEEAIKLSERAVKEYKEGKGKALQAIIGKVMGKTRGKANPELV
                     IKLLKEKLG"
BASE COUNT          611 a          505 c          365 g          784 t
ORIGIN      
        1 ccgtccatgt taatagtagc tcctagaggt agtgcaaaag ctgctatctc tttagagact
       61 ccaatttggt tttcagctaa gtccatgctt actggtaagg ctgcattact actggctgtg
      121 gagaaagcaa tggccatggc aggataaaac ttcttataga atttaaaaat agatagacct
      181 gtaaacgctt ttaatagccc cccgtaaacc agtgtagcat gtattaccaa gcctatataa
      241 acacctaata caacctttaa gattggtact agggctgcca ctcctacagt agcaaaggtt
      301 ctagctacta agctaaagac acctactggt gctaagacca taaccattct taccaattcc
      361 attaccagct catttaactg ttcaaataat tctaatacct ttgtgccttt ttctcctata
      421 atggataggg caattccaga taagattgca aatactataa tttgtagcat gctgccttca
      481 cttaaagatg caacagggtt tgttggtacc atttcatata aaatttgcac caatggtgta
      541 gtttcacttt cggaaacatc aaccgcgtct atagccccca tatctagacc tacgcctgga
      601 ttaattaaga atgctataac taatgatatt acaattgcaa tggcagtagt cactagataa
      661 aaggatatgg ttttgacgcc tacccttcct aattttttga tgtcacccat gctggctgca
      721 ccatttacca aggaaataaa taccaatggt actactagca tcataattga cctgaggaaa
      781 atctggccca ccagttggaa gagtccatta attaaaaagg catctttcaa ggtaccatct
      841 gggaatccat acactataat tcctacaaca acacccagtg ccaatcctat caggaccctt
      901 gtagctagcc ccattctctt tttctccata aaaaccctcc ttatatttta tatttctcct
      961 atctttatta tagaatagga ccatttgttt gtcaatttag aattgtctga aatatgtgtt
     1021 aactactaag aactatcttg gaagagtata atttaaaatt atcctaattt ttctttcaaa
     1081 agcttgatta ctaattcagg attagccttg cccctggttt ttcccataac cttgcctatt
     1141 atggcctgta gggccttacc tttaccttcc ttgtattcct tcactgctct ttcacttaat
     1201 tttatggcct cttctactat aggaagcagg gtttgcctat catttatttg ctctaagtct
     1261 tgttctttta caattattgc aggagatttt ttactcttta ccatttcatc taatactttc
     1321 ttgctggtac tagtatttat tcttccttct gccaataagt tcaccagctc tgccaaatcc
     1381 ttaggttcaa agggcacctt aaagtcatct agatctgtta ttctaaacac ttctgtgatc
     1441 ataagattgg ccaaaatttt tgcatcctgg cttgatttgg ctgcctcttc aaagtagttt
     1501 gccacatctt tagatgaaat aagctgctct gcatcatagc tatttagccc atacttttcc
     1561 atataggttt tcttacgcac atctggcagc tcaggtaggg aggcttctaa ttttttaacc
     1621 ctttcctcat ctatcacaat aggcatcagg tctggatctg gcatgtagcg gtagtcttga
     1681 gaatcctcct tactccgcat agaaaaggtc ctacctgtat tttggtcaaa ccttctagtt
     1741 tcttgtatta tctctcttcc ttccttaatt tcttgtattt gtcgttgggc ttcatattct
     1801 atggacctca taatagattg gaaggaattt aaatttttca tctcagttct agttccatac
     1861 tctttatggc ctttttccct gacagatagg tttacatcac atctaaagga gccttcattc
     1921 atcttgcaat cagatatatt aaggtaaagc ataatggccc ttaatttctg taggaaggct
     1981 ctggtttcct ctgctgagcg caagtctggt tcggatacaa tttcaattaa gggaactcca
     2041 gccctattgt agtctactag gctacctact ccatccttat gaattaactt tccagcatct
     2101 gcctctatat gaattcttgt aatccctatc ctttttgttc tgcctcctac ttctatatcc
     2161 aggtagcccc ctttacatag gggtaaatca tcctgagata tttgatagga gttaggcagg
     2221 tctggataaa agtaatgttt cctgtcctgt tttcctatcc tggtt
//
LOCUS       DATGSI010000206         3836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_61953, whole genome shotgun sequence.
ACCESSION   DATGSI010000206 DATGSI010000000
VERSION     DATGSI010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61953"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1102
                     /gene="spoIIIAA"
                     /locus_tag="VK031_03120"
     CDS             128..1102
                     /gene="spoIIIAA"
                     /locus_tag="VK031_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HLS52936.1"
                     /translation="MPREINTFENAIKYICTDIRSLLLKLPDSYKRNIEEVRLRNGQP
                     LNIYAQGRDFFISKKGTICQSKDGFVVDHKHIKNSFQLITNHSVYAYTEEIRKGFITI
                     SGGHRVGIGGKVIYGEKGVESISDLSSLNIRIAREKKGISDHILPYLIGKDGEFVNTL
                     IVSAPQCGKTTLLRDLIRNLSNGIAGINNGFKIGLVDERSEVAGIYNGQAQKDVGVRT
                     DILDGCFKADGIMMLIRSMSPEIIAVDEIGGKKDIKAIDEGLRAGIRFIATIHGFDLA
                     DIKSRSNLDRLINEKVFKRYIILDKSKGVGTISSIIEGENFKKIFPKW"
     gene            1109..1633
                     /locus_tag="VK031_03125"
     CDS             1109..1633
                     /locus_tag="VK031_03125"
                     /inference="COORDINATES: protein motif:HMM:NF021090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HLS52937.1"
                     /translation="MNTIKLFFSLAILTSSSLLGFLYGGTYGKRAKSLSDLEYCLRVL
                     ESQVVIGNTPLPTALENTYKQGKGDIRLIFKRVKDNLLLEKQEDIYFSFLSLSEELRN
                     KYLLKDADIEVILFLGKILGKSNREDQKKNFQFILGEINEIYKDASKEKDVYEKLYRS
                     LGILLGIGIIIIII"
     gene            1646..1843
                     /gene="spoIIIAC"
                     /locus_tag="VK031_03130"
     CDS             1646..1843
                     /gene="spoIIIAC"
                     /locus_tag="VK031_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HLS52938.1"
                     /translation="MDVDLIFKIAGIGMVTAVLHTVLAKSGKEEYAYLATLVGIVVVL
                     GVVINLVSKLFDNVKVLFKLY"
     gene            1853..2239
                     /gene="spoIIIAD"
                     /locus_tag="VK031_03135"
     CDS             1853..2239
                     /gene="spoIIIAD"
                     /locus_tag="VK031_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HLS52939.1"
                     /translation="MEIFQVVGLGLVATTLIIVLRDHRPEFTIYISIVTGLIIFSMIM
                     PHLSYVIDIMQDLSRKADIEIIYFSTILKIIGMAYVVEFGSQICRDAGENSIAMKIEL
                     AGKITIMILAIPILMALVDLIIKILP"
     gene            2251..3399
                     /gene="spoIIIAE"
                     /locus_tag="VK031_03140"
     CDS             2251..3399
                     /gene="spoIIIAE"
                     /locus_tag="VK031_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HLS52940.1"
                     /translation="MKRNYIVFIFFSIILFISNPLYAEGLDQNQLSIDTGEIDDFVRE
                     IFKENHMDLGGDLKELATKIVKGEKILPRENIMKSISSVFFSELKLSLSLLSKILVVT
                     IASTILTNLQTSFERSSIAQLANYITYILIAIMVITSFTQIMEMARTSIGKMTSLMQV
                     LLPVLLTLLVIAGGPNTKIVFHPMILAAVNVLGIMIKNIIFPLIYFSFIISILSNLSE
                     KTELGKLSEIGRQIATFIISAAFTIFIGILTIYGLSTKIDGISIRTAKFAVDTFVPIV
                     GGFLSDAVDAVIGSTTILKNGIGIVGLLVLILIILTPIIKLTVLLLVFLVTGAVIEPI
                     ASKNITDFFGDISKTVLLILVSLLSIGIMFFITITMIVDAGNSLLMLR"
     gene            3408..>3836
                     /gene="spoIIIAF"
                     /locus_tag="VK031_03145"
     CDS             3408..>3836
                     /gene="spoIIIAF"
                     /locus_tag="VK031_03145"
                     /inference="COORDINATES: protein motif:HMM:NF021121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HLS52941.1"
                     /translation="MGISQFIYSWLENIVVVFVIITLFNLVMPKGNLKRLVNLVIGLL
                     VIYVVISPFLQLSNLELQLEQQVFNHVQTYSSQLDSQFIEGQNKEIENLYKEKIADQI
                     ALTVESNSTYKVQQVEISIYDDEEKYGHLKKIDLYLEEEKD"
BASE COUNT         1342 a          497 c          750 g         1247 t
ORIGIN      
        1 aaaaagagtt taaagcttaa gctttaaact ctttttttat gcctaattat atatttcctt
       61 ttgaaatata taattaggca tatttaatta cttaattcat agtatttata taagggaggg
      121 acatgttatg cctagggaga taaatacttt tgagaatgcg attaaataca tatgcacaga
      181 cattagaagc ctccttttaa agctacctga tagttataaa agaaatatag aagaagtaag
      241 attaagaaat gggcagccac taaacatata tgcccaaggt agggattttt ttataagtaa
      301 aaaagggaca atttgtcaga gcaaagatgg ttttgtggtg gaccataagc atattaaaaa
      361 tagtttccaa ttgattacta atcattctgt atatgcctat actgaggaga tcaggaaggg
      421 atttataact attagcggtg ggcacagggt tggcataggc ggtaaagtaa tttatggtga
      481 aaaaggagta gaaagcattt cagatctatc ctccttaaat attaggattg caagggaaaa
      541 gaaggggatt tcagaccata ttttgcccta cttaattgga aaggacggag aatttgttaa
      601 caccttaata gtatcggctc cccaatgcgg aaaaaccacc ttattaaggg atttaattag
      661 aaatctaagt aatgggattg caggaataaa taatgggttt aaaataggtt tggtagatga
      721 aagatcagag gttgctggta tctacaatgg ccaggcccaa aaggatgtag gtgttaggac
      781 tgatatttta gatggttgct ttaaggctga cggcataatg atgctaatcc gctccatgtc
      841 gcctgaaata atagctgttg atgaaatagg tggtaaaaaa gatataaaag caatagacga
      901 aggactaagg gctggtatca ggtttatagc aactattcat ggatttgatt tagcagatat
      961 taaaagcagg tctaatttag acaggttaat taatgaaaaa gtatttaaaa gatatattat
     1021 cctagataaa agtaagggag taggaaccat aagttcaata atagagggag aaaattttaa
     1081 gaaaatattt ccaaaatggt gagtgtttat gaatacaata aaattatttt ttagtttagc
     1141 tattttaaca agctcctcct tgttaggctt tctttatggg ggaacctatg gaaaaagggc
     1201 caagtcattg tcagatttag aatactgctt gagagtattg gaaagccaag ttgtaatagg
     1261 aaatactccc ctgcctacag ctttggaaaa tacatataaa cagggcaagg gagatataag
     1321 attaatcttt aaaagggtaa aagacaatct tttattagaa aaacaggaag atatatattt
     1381 tagtttttta tcattaagtg aggaattaag gaataaatat ttattaaaag atgcagatat
     1441 tgaggttata ttatttcttg ggaagatact gggaaaatca aacagagagg atcaaaaaaa
     1501 gaactttcaa tttatcttag gtgagatcaa tgaaatatat aaggatgcca gcaaagaaaa
     1561 ggatgtatat gaaaaactgt atcgttcctt aggaattttg ctaggaatag gtataataat
     1621 tatcattatt taggagagat gaactatgga tgtagattta atatttaaaa tagcaggaat
     1681 tggaatggtc actgctgtac tccatacagt tctagctaag tcaggcaagg aggaatatgc
     1741 atatttggcc acattagtag gaattgttgt tgtattaggt gtggtaatta atttggttag
     1801 caagctattt gataatgtta aggtcctgtt taagctttat tgaggtgatg gtatggagat
     1861 ttttcaagtg gtaggcttag gcttggtggc tacaacatta ataattgttt taagagatca
     1921 taggccagaa tttaccatat atatttctat agttacaggc ctgattatat ttagcatgat
     1981 tatgcctcac ctatcctatg taattgatat tatgcaggat ttatctagaa aggcagatat
     2041 agaaattata tatttttcca ccatattaaa aatcatagga atggcctatg ttgttgaatt
     2101 tggatcccaa atatgtaggg atgcaggtga aaattcaata gccatgaaaa ttgagctggc
     2161 agggaaaatc accataatga tcctggcaat tcccatactg atggccttgg tagacttaat
     2221 aataaaaata ttaccttaag gatgaagaaa atgaagagga attatatagt atttatattt
     2281 ttttctatta ttttatttat ttcaaatccc ctgtatgctg aaggattgga tcaaaaccaa
     2341 ttatctattg atacaggaga aatagatgat tttgtaagag aaatatttaa ggaaaatcat
     2401 atggatcttg ggggagatct aaaagagctg gctacaaaaa ttgttaaagg agaaaagatt
     2461 cttcctagag aaaatataat gaaaagtata agctctgttt ttttcagtga attgaagcta
     2521 tctttatccc ttttatctaa aattttagtt gttaccatag catctaccat attaacaaat
     2581 ctacaaacaa gttttgaacg atcttccata gctcaattgg ccaactacat tacctatatt
     2641 ttgatagcca ttatggtgat aactagcttt acccagataa tggaaatggc aaggacatcc
     2701 attggtaaga tgacaagctt gatgcaggtt ctattaccag ttctattaac ccttttagtt
     2761 attgctggcg gtcccaatac aaaaatagtt tttcatccta tgatattggc tgctgttaat
     2821 gtgttaggta ttatgataaa aaatataata tttcctttga tttatttttc cttcatcata
     2881 agcatattat ctaatttatc tgagaaaaca gaactaggta aactatctga aataggtaga
     2941 caaatagcca cctttatcat atcagccgcc tttacaattt ttatagggat tttaaccata
     3001 tatggcttat ctactaagat agatggaata agtattagga ctgccaaatt tgcagtggat
     3061 acctttgtcc ccattgttgg tggatttttg tctgatgctg ttgatgcagt tattggatca
     3121 acaaccatat taaaaaatgg aattggaata gtaggcttat tggtactaat cttaattata
     3181 ttaactccaa tcattaaact tacggtccta ttgctagtct ttctggtaac aggtgcagta
     3241 atagaaccta tagccagtaa aaatataact gatttctttg gggatatttc aaaaacagtt
     3301 cttcttatct tggtcagcct attgtccata ggtattatgt ttttcattac tataaccatg
     3361 atagtggatg caggtaatag cctgcttatg ttaaggtagg tggtttcatg ggcataagcc
     3421 aatttatata ttcttggtta gaaaatatag tagttgtatt tgtcataata accttattta
     3481 acctagttat gcctaaaggg aatttaaaaa ggctggttaa cctggttata gggttattgg
     3541 taatttatgt agtaatttct ccatttttgc aactgtctaa tttagagctt cagctggagc
     3601 agcaggtctt caaccatgtt caaacctact catctcaact agattctcaa tttatagagg
     3661 gacaaaataa ggaaattgag aatttgtata aggaaaagat agcagatcaa atagctctaa
     3721 cagttgaaag taattccacc tataaagtgc aacaagtgga gatttctata tatgatgatg
     3781 aggaaaaata tggccactta aaaaagatag acctatacct ggaggaagaa aaagat
//
LOCUS       DATGSI010000207         1483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_248375, whole genome shotgun sequence.
ACCESSION   DATGSI010000207 DATGSI010000000
VERSION     DATGSI010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248375"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VK031_03150"
     CDS             <1..455
                     /locus_tag="VK031_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52942.1"
                     /translation="RMGDSWTYEPYERFDVVFPDGSKTEYGRLNRTGVTWDNEFQVFT
                     VNSDIEESATRSEDISMDYDFFHSELLPLSCGNDYTVKIIPKDINIWLSRLFLGDADG
                     FSILYYQDVDSLVHWSNEAAYRWKLRGIAIWSLGQEDMRIWEALPKQI"
     gene            540..947
                     /locus_tag="VK031_03155"
     CDS             540..947
                     /locus_tag="VK031_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HLS52943.1"
                     /translation="MKEIWNWVQIALTALGGFLGWFLGGFDGFLYALVALMVADYITG
                     VMCAIVDKTLSSEVGFRGIFKKVLIFVLVGIGHIIDTNLIGDGNVLRTAIIFFYCSNE
                     GVSMLENASRLGLPIPEKLKDVLAQLHNKGGNN"
     gene            947..>1483
                     /locus_tag="VK031_03160"
     CDS             947..>1483
                     /locus_tag="VK031_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HLS52944.1"
                     /translation="MNLRKLIFTNNACYKAGRKIKPKGIMVHSTGANNPSLRRYVGPD
                     DGLLGKNQYNNHWNQDRPDGRQVCVHAFIGKLKDGSIATYQTLPWDHRGWHAGGDANN
                     THIGFEICEDNHTDATYFNKVYKEAVELCVHLCKLYNLTEKDIIGHYEGHQKGIASNH
                     SDPRNWFPKHGKSMDTFRA"
BASE COUNT          457 a          224 c          335 g          467 t
ORIGIN      
        1 taagaatggg cgattcatgg acttatgagc catatgaaag atttgatgtg gtatttccag
       61 atgggagtaa aactgagtat ggaagactta atagaactgg tgtaacttgg gataatgagt
      121 ttcaagtttt tactgttaat agtgatatag aagaatctgc aacgagaagt gaagatattt
      181 ctatggacta tgactttttc cattcagaac ttttacctct tagctgtgga aatgattata
      241 ccgtcaagat tattccaaag gatatcaata tttggttatc aagacttttc cttggtgatg
      301 ctgatggctt ttctattctc tattatcaag atgtagatag tcttgttcac tggtcaaatg
      361 aagcggctta tagatggaaa cttaggggaa ttgccatctg gtctttaggt caagaagata
      421 tgaggatttg ggaggcactc cctaaacaaa tataacaata aatatctatt caggagtatt
      481 tgcaagtttt gcagatgctc cttttttata tacaaattaa atttgaggag gtcctatgta
      541 tgaaggaaat ttggaactgg gtacaaattg ctctcacagc cctcggtggc tttttaggtt
      601 ggtttctcgg aggttttgat gggttcttat atgctttagt tgctttgatg gtggcggatt
      661 atatcactgg ggttatgtgt gccattgtag ataaaactct atccagcgaa gttggattca
      721 gagggatatt taaaaaggtg ctgatctttg tattagttgg gattggccat atcatcgata
      781 caaatcttat cggggacgga aatgtgctta ggactgctat tatctttttc tactgctcta
      841 atgagggggt ttccatgctt gaaaatgcat caagacttgg acttccaata ccagagaaat
      901 taaaggatgt cttagctcag ctacacaata aagggggaaa taattaatga atttaagaaa
      961 gcttattttt actaataatg cttgttataa ggctggaaga aagattaagc ctaagggaat
     1021 tatggtacac tcaactggtg caaacaatcc tagccttaga aggtatgtcg gtccagatga
     1081 tggactactt ggtaaaaatc aatataataa tcattggaat caagatagac cagatggcag
     1141 gcaagtctgc gttcatgcct ttattggcaa gcttaaggat gggtctatcg ccacttatca
     1201 aaccctgcct tgggatcata ggggatggca tgcaggtggt gatgctaaca atacgcatat
     1261 aggttttgaa atctgtgaag ataatcatac tgatgctacc tactttaata aggtttataa
     1321 agaagctgtc gaactatgtg tgcatctttg caagctctat aatcttactg aaaaagatat
     1381 cattggacac tatgaaggac atcaaaaagg aatagccagt aaccacagcg accctagaaa
     1441 ttggtttcca aagcacggta aaagtatgga tacttttagg gca
//
LOCUS       DATGSI010000208         1559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_535973, whole genome shotgun sequence.
ACCESSION   DATGSI010000208 DATGSI010000000
VERSION     DATGSI010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535973"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1172)
                     /locus_tag="VK031_03165"
     CDS             complement(318..1172)
                     /locus_tag="VK031_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLS52945.1"
                     /translation="MKAQSTSFEADEGYELGEIIGQNGWGGFSPLSESYLVSDDMATD
                     GDYSLKIGLDFLGIIPSGSVIGPTRDISQQVPNAPARYEVSADLYITSSEEGGEIDFS
                     VYGAGGTESLPAATIALLDGRVLIIELSDFSVGADIDVPTETFIRLSMLFDFENEETL
                     YFIDEDLVYTGNLNLSRVTGYGFLSTGKAIGYVDNITATDNPTLGVTDVESSIFSHYV
                     DNGKLYLKSHSIIEEVSIYNLNGQKVLAEAFHLTDGIVELDALTSGVYLVRLKLENES
                     RTFKFFKQ"
BASE COUNT          508 a          298 c          250 g          503 t
ORIGIN      
        1 actgacttgg tagatataca aatcacttta cagccattgg cggtggttaa agcataggta
       61 attcaggata taaatgaaga gaattatatt tatgctataa aattatagtc tgtatctagg
      121 taaccaaagc attgggatat aggcgctgat gcttttcaac ttaaagtgat agtaataagt
      181 ataaaagaat tcttaattat tttactctgt agcttttact aggctaaaga ataaattaaa
      241 cccgaaaaca gcattaatag acttggataa tgtcattttc gggtttttgt attctccttt
      301 aataaagaga gtatggatta ctgcttaaaa aacttaaaag ttctactttc gttttcgagt
      361 tttaaacgaa ctaagtaaac acctgaggtt agggcatcta actcaactat tccatcagtg
      421 agatgaaaag cttcagctaa tactttttgt ccatttaggt tgtatatgct cacctcttct
      481 attatagaat ggcttttaag ataaagtttt ccgttatcta cataatgaga gaatatactt
      541 gattctacat cagtaacacc taaagttgga ttgtcggtag cagttatatt atcaacataa
      601 ccaatcgctt ttccagtgga aagaaaacca tatcctgtaa ctcgagaaag atttaaattt
      661 ccagtatata ctaaatcctc gtcgataaag taaagtgttt cttcattttc aaaatcaaac
      721 aacatggata aacgaataaa agtttcagta ggaacatcta tgtcggctcc taccgaaaaa
      781 tcagatagct caataattag cacccttcca tcgagcaagg ctatggtagc agcgggtaaa
      841 gactcggttc cacctgctcc atatacacta aagtctattt cccctccttc ttctgacgag
      901 gtgatataaa gatcggcaga tacctcgtag cgagctggtg cattaggtac ctgttgactt
      961 atatctctgg tagggccaat taccgatcca ctaggaatga ttcctaaaaa atccaatcca
     1021 atttttaatg aataatcgcc atcggttgcc atgtcatcgg ataccaaata actttcggat
     1081 aaagggctga atcctcccca accattttgc ccgataattt cgcctaattc atagccttca
     1141 tccgcttcaa aagaagtgct ttgagccttc aattagccaa taataagtaa tgtggataat
     1201 acaaataaaa gttttttcat agatataaat ttttggccaa aaataattaa actagcttag
     1261 aaagaatatt ctatacctgc tattcttctt attctattaa gaaaaccaag accacctcat
     1321 tttttcattc tatttcactc ctaatttaaa ccagactggt tgaatgctaa atagctttct
     1381 agcaaatatt ttattctgtt catcgaagat taggtcgcga ccatttaggc ttttctccta
     1441 aagatttaaa ctgagagtcc ttagcttcca cagtaaccgg ctgcttagct ttctcgaaag
     1501 gcttttgagg ttttaacccc aattctttaa acagctccat atcctcctca atatcaggg
//
LOCUS       DATGSI010000209         4040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_296945, whole genome shotgun sequence.
ACCESSION   DATGSI010000209 DATGSI010000000
VERSION     DATGSI010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296945"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..271)
                     /gene="rpmD"
                     /locus_tag="VK031_03170"
     CDS             complement(95..271)
                     /gene="rpmD"
                     /locus_tag="VK031_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HLS52946.1"
                     /translation="MAKIQIKLVRSPIGKPENHKRIVKALGFRKVGQIIEKNDTPQIR
                     GMINKINHMVEVVE"
     gene            complement(283..789)
                     /gene="rpsE"
                     /locus_tag="VK031_03175"
     CDS             complement(283..789)
                     /gene="rpsE"
                     /locus_tag="VK031_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HLS52947.1"
                     /translation="MERSFINANELDLKERVVNINRVTKVVKGGRNFRFSALVVVGDE
                     KGHVGVGMAKALEVPEAIRKAIQDAKKHIVKVPLIGTTIPHEIIGNYGAGKVLLKPSK
                     EGTGVIAGGAVRAVCELAGIRDIRTKSLGSSNPRNIVNATMDALQNLKTAEDVARLRG
                     KTVEEILG"
     gene            complement(806..1168)
                     /gene="rplR"
                     /locus_tag="VK031_03180"
     CDS             complement(806..1168)
                     /gene="rplR"
                     /locus_tag="VK031_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HLS52948.1"
                     /translation="MLKKTDRNLNRKARHRRVRKKIFGTPERPRLNVFRSSKHIYAQV
                     IDDTTGSTLASASTLDKSLNLTSTNNKEAARAVGELVAKRALEKGIEEIVFDRGGYIY
                     HGRVKELAEGAREAGLRF"
     gene            complement(1185..1724)
                     /gene="rplF"
                     /locus_tag="VK031_03185"
     CDS             complement(1185..1724)
                     /gene="rplF"
                     /locus_tag="VK031_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HLS52949.1"
                     /translation="MSRIGLRPINIPSGVEINIKENNVVEVKGPKGQLTQQIDPAMKL
                     KIEDNILTIERPTETKRHKSLHGLSRTLIANMIEGVTEGYSKELEIIGTGYRAQKQGK
                     KLILGLGFSHPIELEDPEGIEVEVPAANKIIVRGIDKQKVGNYAAVIREFRKPEPYKG
                     KGIRYADEQVRRKVGKTGK"
     gene            complement(1761..2156)
                     /gene="rpsH"
                     /locus_tag="VK031_03190"
     CDS             complement(1761..2156)
                     /gene="rpsH"
                     /locus_tag="VK031_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HLS52950.1"
                     /translation="MMTDPIADMLTRIRNGNDARHKSVDIPASNIKKQVAQILLDEGY
                     IKGFNVIDDGKQGIIRIDLKYGNNNEKVISGIKRISKPGLRVYVKNNEVPRVLGGLGI
                     AILSTSSGVMTDKSARKHGVGGEVLCYVW"
     gene            complement(2186..2371)
                     /locus_tag="VK031_03195"
     CDS             complement(2186..2371)
                     /locus_tag="VK031_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HLS52951.1"
                     /translation="MAKKSMIAKQKRKQKFSSREYNRCKICGRPHGYLRKYGVCRICF
                     RELAYKGQIPGVRKASW"
     gene            complement(2388..2930)
                     /gene="rplE"
                     /locus_tag="VK031_03200"
     CDS             complement(2388..2930)
                     /gene="rplE"
                     /locus_tag="VK031_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HLS52952.1"
                     /translation="MASRLKEKYENEVVKKLMEKFEYKNVMEVPRLEKIVVNMGVGEA
                     KENPKLLESAVRELAAITGQSPVVTQAKKSIANFKLREGMNVGTKVTLRGEKMYDFLD
                     KLMNVALPRVRDFRGVSPTSFDGRGNYALGIREQLIFPEIEYDEIEAIRGLDIVIVTT
                     AETDEEARVFLQEMGMPFRK"
     gene            complement(2951..3265)
                     /gene="rplX"
                     /locus_tag="VK031_03205"
     CDS             complement(2951..3265)
                     /gene="rplX"
                     /locus_tag="VK031_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HLS52953.1"
                     /translation="MHVKSGDKVVVISGKDVGKIGKVLKAFPNENRVIVEGVNMLTKH
                     KKPMGPTEPGGIVKYEGPIHASKVMLYCDNDEQGVRFGYNILKDGSKVRVCKKCGEVL
                     DK"
     gene            complement(3338..3706)
                     /gene="rplN"
                     /locus_tag="VK031_03210"
     CDS             complement(3338..3706)
                     /gene="rplN"
                     /locus_tag="VK031_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HLS52954.1"
                     /translation="MIQVESRVRVADNSGAREVLVIRVLGGTNRKYAGIGDVVVCSVK
                     SATPGGVVKKGEVVKAVVVRTKDGVRRDDGSYIKFDDNAAVIIKEDRSPVGTRIFGPV
                     TRELRRENFMRIISLAPEVL"
     gene            complement(3734..3988)
                     /gene="rpsQ"
                     /locus_tag="VK031_03215"
     CDS             complement(3734..3988)
                     /gene="rpsQ"
                     /locus_tag="VK031_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HLS52955.1"
                     /translation="MERGNRKVRIGTVVSDKMDKTVVVAVETFVTHPLYKKQIRRTSK
                     FKAHDENNECGIGDVVRIMETRPLSKHKNWRVVNIIEKAK"
BASE COUNT          982 a          997 c          479 g         1582 t
ORIGIN      
        1 atccagcacc tcttcctacc ctttttcttg gcttgctacc tccgccttcg ttcggtctta
       61 attcatgtaa tttcatgagt gcacctcctt acacttattc taccacttct accatatggt
      121 ttatcttgtt tatcattcct ctaatttgag gggtatcatt tttttctatt atctggccta
      181 cttttctaaa tccaagggcc tttacaattc ttttatggtt ttcaggtttt cctattggac
      241 ttctaaccag cttaatttga atcttagcca tttaaatccc ccctaaccta atatttcttc
      301 tactgttttt cctcttaatc tagctacatc ttctgctgtc tttaagtttt gtaaggcatc
      361 catagttgca tttactatat ttcttggatt actacttcca agggattttg tcctaatatc
      421 tcttatgcct gccaattcac atacagcacg cactgctcca ccagctataa ctcctgtacc
      481 ctcttttgat ggtttaagca ataccttacc tgcaccatag ttacctatta tttcgtgagg
      541 gatagtagtt cctatcagcg gaacctttac aatatgcttt ttagcatctt ggattgcctt
      601 tctaatagcc tcaggtacct ccagtgcctt agccattcct actcctacat gccctttttc
      661 atctccaaca actaccaggg cgctgaatct aaagtttcta ccacctttaa caactttagt
      721 tacacgattt atattaacta ctctctcttt taaatctaac tcatttgcat taataaatga
      781 acgctccatc atttccctcc ttcagttaga atctaagacc tgcctccctt gctccttcgg
      841 caagttcctt tactcttcca tgatagatgt atccacctct atcaaatact atctcttcta
      901 tacctttttc cagtgccctt ttagcaacca attctcctac tgccctagcc gcttctttat
      961 tgttagttga agtaaggttt agtgacttat ctaaagtaga agctgatgct aatgtagagc
     1021 ctgttgtatc atctataacc tgtgcataaa tatgtttaga acttctgaaa acatttagtc
     1081 ttggtctttc aggcgttcca aagatttttt ttcttactct acgatgtctt gcctttctgt
     1141 ttaagtttct atcagtcttt tttagcatta agtttcactc ccttctactt accagtttta
     1201 ccaaccttac gtcttacctg ttcgtctgca tatcttatac ccttaccctt gtaaggttca
     1261 ggctttctaa attccctaat tactgcagcg tagttaccta ccttctgctt atctattcct
     1321 cttacaataa tcttgtttgc agctggcact tctacttcaa ttccttctgg atcttctagc
     1381 tcaattggat gggaaaagcc taatcctaaa atcaactttt taccttgttt ttgtgcccta
     1441 tatcctgttc caatgatttc taattcctta gaatatcctt cagtaactcc ttctatcata
     1501 ttagctatta atgttcttga taacccatgt aaagatttgt gtcttttagt ttctgttggt
     1561 ctttctatag ttaaaatatt gtcctctatt ttaagcttca tagctggatc aatttgctga
     1621 gttagctgcc ccttgggccc ctttacctca acaacattat tttctttaat atttatttcc
     1681 actccacttg gaatgttaat tggtcttaat cctattcttg acatgagtgc acctcctgtt
     1741 cctctagtct tctatatttt ttaccataca taacaaagta cttctccacc tacaccgtgt
     1801 tttctagcac ttttgtctgt cataacccca cttgatgtgg atagaatagc tatacctaag
     1861 ccacctagta cccttgggac ctcgttattc ttaacatata ctcttaaacc aggctttgag
     1921 atcctcttta taccactaat aactttttcg ttattgttgc cgtactttag gtctatccta
     1981 ataattcctt gcttgccatc atctattaca ttaaagccct ttatatatcc ctcgtctaaa
     2041 agaatttggg ctacttgctt ttttatattt gaagctggaa tgtcaactga tttatgtctc
     2101 gcatcattac catttcttat cctagttagc atatctgcaa ttgggtctgt catcatttta
     2161 gctacctcct ttcataagct taaaactacc aacttgcttt tcttactcca gggatctgcc
     2221 ctttatatgc tagttctcta aagcatatac ggcatactcc atacttcctt aaataaccat
     2281 gtggtctacc acatatttta catctattgt attctctgga gctaaacttt tgttttcttt
     2341 tttgcttagc tatcattgac tttttagcca ttctttatcc ctcctcatta ttttctaaag
     2401 ggcattccca tttcttgtaa gaatactcta gcttcttcat cagtttcagc cgttgtaaca
     2461 attactatat ccaaacctct tatagcctct atctcatcgt attcaatttc tgggaaaatt
     2521 agctgttccc taatacctaa ggcatagttg cctcttccat cgaaacttgt tggacttaca
     2581 cctctaaagt ccctaaccct aggtaaagca acattcatta acttatctag gaagtcatac
     2641 attttttccc ctcttaaggt aactttagtt ccaacattca ttccttctct taatttaaag
     2701 tttgctattg atttctttgc ctgggtcact actggacttt gacctgtaat agcagctaat
     2761 tctcttacag ctgattcaag tagttttgga ttctcttttg cttctccaac tcccatgttt
     2821 acaacaattt tttcaagtct cggtacttcc ataacatttt tatattcaaa cttttccatt
     2881 agctttttta ctacttcatt ttcatatttt tcctttaatc tggaagccat attaattacc
     2941 tcccctctga ctatttatct agaacttctc cacatttttt acatactctt accttgctac
     3001 catctttcag tatattataa ccaaacctaa ctccctgctc gtcattgtcg caatataaca
     3061 taactttgga agcatgaata ggcccttcat attttactat tccacctggt tctgttggtc
     3121 ccattggttt cttatgcttt gtaagcatgt ttacgccttc cactattact ctgttttcat
     3181 ttgggaatgc ttttaaaact tttccgatct tcccaacatc tttgcctgaa ataactacta
     3241 ccttatctcc actttttaca tgcatacttt acacctccta acaagtacta tgggcttttg
     3301 gcttatgcat aattaattta aaatttataa tctattatta tagaacctct ggcgccagtg
     3361 atattatcct cataaagttt tctcttctta actctcttgt tacaggacca aatatacggg
     3421 ttcctaccgg acttctatct tccttgataa taactgctgc attatcatca aatttaatat
     3481 aggaaccatc atctcttctc acaccgtctt tagtccttac aactactgct tttactacct
     3541 ctcctttttt aacaactcca ccaggcgttg cacttttcac agaacaaact actacatctc
     3601 caatacctgc gtatttcctg tttgttcccc caagcactct tataactaaa acctctctgg
     3661 ctcctgagtt atcagctacc ctaacacgac tttctacttg gatcattaga atacctcctt
     3721 tcatgtataa ggactatttt gccttttcta ttatatttac aactctccag tttttatgtt
     3781 tgctcagtgg cctagtttcc ataatcctta ccacatcacc tataccacat tcattgtttt
     3841 catcatgagc tttgaacttt gaagtccttc ttatctgctt cttatataaa ggatgtgtaa
     3901 caaaggtttc aacagctaca acaaccgttt tatccatctt atcacttact actgtaccta
     3961 ttctaacctt acgattacct ctttccattt gagattaaac ctcctttcct atacctaatt
     4021 ctcgttctcg aataatagtt
//
LOCUS       DATGSI010000210         4207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_158933, whole genome shotgun sequence.
ACCESSION   DATGSI010000210 DATGSI010000000
VERSION     DATGSI010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158933"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VK031_03220"
     CDS             <1..781
                     /locus_tag="VK031_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52956.1"
                     /translation="YLDGQILGGVVEENENAQVEIKVIGDSAIDRIDVLKDNIVADTY
                     IHSGKWERRPIDGKFRLKVQTEFGWGPDMRIFTDIYKKNWDVKMTTTGKIISVQNNWN
                     SMGQEVTYIGDKEFKCSLTTFKATDTGKWMGTSHVKQEGFTFELEGSQEDRVTFYVDD
                     KVYEYPYKELLATSHLHGDLDASHKLIEERFGKITSHRNDSWWHNAYKFKVHQAVPKA
                     AYSCRWETKISTKSAKNVRVKVYQKNGAIAFVSPVFIERKR"
     gene            784..2163
                     /locus_tag="VK031_03225"
     CDS             784..2163
                     /locus_tag="VK031_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HLS52957.1"
                     /translation="MKMNIIYIHTHDTGKVISPYGYNVISPNIEEFFQDATIFYNAFS
                     AAPTCSPSRAALTSGCYPHQVGMLGLAQRGFEMDTDKHMAKLLRDNGYHTVLSGVQHE
                     KEYYLDHDTVYKDLGYLEDVTEEHRHYKEEDLVFWDEENSYLLKKWIKNYKGDKPFFI
                     SYGLHGTHRKYLEEISENVTVDSSVPPPYIYNNPASREDFARYKTSLEITDRNIGRVL
                     KALKEAGYYENSIIFITTDHGVAYPFSKCTLKDRGIAVFLSMRVPSGGDRLKSYDGLV
                     SQIDILPTLLDLVAIEKPAYIEGRSFAKLFTEKAEKTYKVRDEIFAEINFHTSYEPAR
                     CIRTKRYKYIKFFDEYLKVNYSNIDASPIKEFLNQHDLKDMSKPRESLYDLYYDPIEE
                     NNLINDPRYREVLNRLRRRMKEIMRKTKDPLLDGNIKIKEEWKVNKQDAYHASSKDPD
                     DYISLGRKK"
     gene            2163..2633
                     /locus_tag="VK031_03230"
     CDS             2163..2633
                     /locus_tag="VK031_03230"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HLS52958.1"
                     /translation="MEIKNVRIDYRGIHGQVSVSWVSNLKINRIMVIDDKAVKNHMQK
                     SMLKMACPKNVKLSIISTDRAKLRLNEPERYEGENILILILQVETLKTLANKGITFPE
                     VTFGNVSNRPNTSQVSKTVYLTDEEKEIVKNLQEQGTDFYYQMVYGDNKEEIKV"
     gene            2648..3469
                     /locus_tag="VK031_03235"
     CDS             2648..3469
                     /locus_tag="VK031_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HLS52959.1"
                     /translation="MQLWQAILIGLFAYLGRNQVPWLFGTTGGFYAVGRPLVAGLIVG
                     LILGNVEAGILAGIAVQAIFIGQIVPGGAMPSDLNLAAYVGIPLAIVAGGDANTAVGL
                     AVPIGILGVALHNLTMTVNAIWVHRADKYAAEGNARQVRISNILGTLIPFIERFGIVT
                     LTLYFGAGFAESLIDNLPQLVLDFLAVAGRLLPALGFAVLLNQMVAERWTISLFILGW
                     VVMTALEITITSLLLIAIAIALLFAMAADRSGSFNLAGNEDVIIDGGDDDDEYEE"
     gene            3450..>4207
                     /locus_tag="VK031_03240"
     CDS             3450..>4207
                     /locus_tag="VK031_03240"
                     /inference="COORDINATES: protein motif:HMM:NF015570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HLS52960.1"
                     /translation="MTNTKNRDGKSLSKRDVSIAAWRWMFFHHCAQNFERMQGAAYAH
                     TLSGSLEKLYKDDKEGLAEALTRNLVFFNTEPQLGAVIPGISLALEEDYALNPENNDP
                     EIMVNTKNALMGPFAGLGDPLLVGTLNPILLSIGIGMSQGGSVMGAIFFLVAWLAIVV
                     PMKYFLFIRGYGLGIDAIKLISNERLKEMITSILTIVGLIVIGGVASTTVAASIKLTF
                     ESGEMIISIQEILDRVMPKILPLIITLTSFHLIK"
BASE COUNT         1436 a          653 c          934 g         1184 t
ORIGIN      
        1 ttacttagat gggcaaatac ttgggggagt ggttgaagaa aatgaaaatg cccaggtaga
       61 aattaaggtc ataggtgata gtgcaataga taggatagat gtgctaaagg ataacatagt
      121 tgcagatact tatatccact caggcaaatg ggaaagaagg cccatagatg gtaaattcag
      181 gctaaaggtg caaacagaat ttggctgggg acctgacatg agaatattta cagatattta
      241 taagaaaaat tgggatgtaa aaatgacaac cacaggtaaa ataatatctg tccaaaacaa
      301 ctggaatagt atgggccaag aagttaccta tataggagat aaggagttta aatgtagctt
      361 aacaaccttt aaggccacag atactggaaa atggatggga acatcccatg tgaagcagga
      421 aggctttacc tttgagttag aaggaagcca ggaggatagg gtaacctttt atgtggatga
      481 taaggtgtat gaatatccct ataaagaact tcttgccaca tcacacctac atggagactt
      541 ggatgcttcc cacaagctaa tagaggaaag gtttggcaag attacctccc ataggaacga
      601 ttcctggtgg cataatgctt ataaatttaa agtccatcag gcggtgccta aagcagctta
      661 ttcttgtaga tgggaaacta aaatatcaac caaatctgct aaaaatgtaa gggtcaaagt
      721 ttatcagaaa aatggtgcaa tagcttttgt atctcctgta tttattgaaa ggaagaggta
      781 attatgaaga tgaatataat ttacattcat acccatgaca caggcaaggt tatatctccc
      841 tatggataca atgttatctc cccaaatatt gaggaattct ttcaagatgc caccatattt
      901 tataatgcct tcagtgctgc acctacctgt tctccaagta gagcagcctt aaccagcggc
      961 tgttatcccc atcaggtagg catgctaggc ttggctcaaa gagggtttga aatggacaca
     1021 gataagcata tggccaaact tctaagggat aatggctatc atacagtcct atctggtgtc
     1081 cagcacgaaa aagaatatta tcttgatcac gacactgtat ataaagattt aggctacttg
     1141 gaggatgtta ctgaagaaca taggcattac aaggaagagg acctggtgtt ttgggatgaa
     1201 gaaaatagtt acttgttaaa aaaatggata aaaaactata agggagataa gccctttttt
     1261 atatcctatg gtctacatgg gacccataga aaatatctag aagaaatatc tgaaaatgtg
     1321 actgtagata gctcagttcc ccctccttat atttataata atccagccag cagggaggat
     1381 tttgccagat ataaaaccag tttagaaatt acggatagga atataggtag ggttttaaag
     1441 gctttaaagg aagctggcta ctatgaaaac tccattatat ttataactac cgaccatgga
     1501 gtggcctacc cctttagtaa gtgtaccctt aaggatcgtg gtattgccgt atttctatca
     1561 atgagggtgc caagtggtgg agacagactt aaaagttatg atgggctagt ttctcaaatc
     1621 gatattttgc caaccttatt ggatctggta gccattgaaa aaccagccta tattgagggg
     1681 agaagttttg ccaagctttt cactgaaaaa gctgaaaaaa catataaggt aagggatgaa
     1741 atatttgcag aaataaactt tcacacctcc tatgaaccag ctaggtgcat aagaactaaa
     1801 aggtataaat atattaaatt ctttgatgaa tatttaaagg ttaattattc caatatagat
     1861 gcatcgccta ttaaagagtt tttaaaccaa catgacctaa aggatatgtc aaaaccgaga
     1921 gaaagcctat atgacctata ttatgatcca attgaggaga ataatcttat taatgatcca
     1981 aggtatagag aggtgttgaa taggcttagg agaaggatga aagaaataat gagaaaaacc
     2041 aaggatccat tattggatgg aaatattaaa attaaggaag aatggaaggt aaacaaacaa
     2101 gatgcatatc atgccagctc aaaggatcca gatgattata ttagcttagg gaggaagaag
     2161 taatggaaat taaaaatgta aggatagatt ataggggtat tcacggacag gtgtcagtct
     2221 cctgggtttc aaatttaaaa ataaacagaa tcatggtaat agatgataag gcagtaaaaa
     2281 accatatgca aaaatccatg ttaaagatgg cctgtcctaa aaatgtaaag ctatctatca
     2341 tctccactga tagggctaag ttaagattaa atgagcctga aaggtatgag ggagaaaata
     2401 tattaatctt gatcttacag gtggagactt tgaaaacttt ggccaataag ggaataacct
     2461 tccctgaggt aacctttgga aatgtaagta acaggccaaa cactagtcag gtaagtaaga
     2521 ctgtttattt aacagatgag gaaaaagaaa tagttaaaaa cttacaagaa caaggcacag
     2581 atttttatta tcaaatggtt tatggggata ataaggaaga gataaaagta tagaaaggag
     2641 aatttatatg caattatggc aggcaatctt aatcggtcta tttgcatatt tagggagaaa
     2701 ccaggtaccc tggttatttg gaactactgg tggtttttat gctgtaggca gacccctggt
     2761 cgcaggcttg atagtaggcc tgattttagg caatgtggaa gctggaatac tggcaggtat
     2821 agcagttcag gccatattta taggtcagat tgttccaggt ggggccatgc cctccgattt
     2881 aaatctggct gcttatgtag gtattcctct ggcaatagtg gcaggtggag atgctaatac
     2941 agcagtagga ttagcagtgc ctattggaat tttaggagta gccctgcata acctaactat
     3001 gactgtaaat gctatttggg tccacagggc agataaatat gctgcagaag gaaatgctag
     3061 acaggttagg atctcaaata tattggggac attaatccct tttatagaaa ggtttggcat
     3121 tgtaacacta actttatatt ttggggcagg ctttgctgaa agtttaattg ataacttacc
     3181 acagttagta ttagactttt tggctgtagc tggaaggcta ttacctgccc taggttttgc
     3241 agtgttatta aatcaaatgg tagcagaaag atggacaatt tcacttttta tcctagggtg
     3301 ggttgtcatg acagccctag agataaccat aacatccctg cttttaatag ctatagccat
     3361 agccctctta tttgctatgg cagcagatag gtcaggtagc tttaacctag caggaaatga
     3421 agatgtgatt atagatggcg gtgatgacga tgacgaatac gaagaataga gatgggaaaa
     3481 gcttaagtaa aagggatgta tcaatagcag cctggcgctg gatgtttttc catcattgtg
     3541 ctcaaaactt tgagaggatg cagggagcag cctatgccca caccctatca ggcagtttag
     3601 aaaaacttta taaggatgat aaggaaggat tggcagaagc cttaacaaga aatctagtgt
     3661 tttttaatac agagcctcaa ttgggggcag taataccggg tatttcattg gctttggaag
     3721 aggattatgc tttaaatcct gaaaacaatg atccggaaat tatggtaaat actaaaaatg
     3781 cactaatggg gccttttgcc ggcctagggg atcctctatt agtaggaact cttaatccaa
     3841 tacttttaag tataggaata gggatgagcc aaggtggctc agttatgggg gcaatatttt
     3901 tcctagtagc ctggttagct attgtagttc ctatgaaata ttttctattt attaggggct
     3961 atggccttgg tatagatgct attaaattaa tcagcaatga aagattaaaa gaaatgataa
     4021 catctatcct tacaattgta ggtttaatag taattggtgg tgtggcctcc acaactgtag
     4081 ccgcctctat taaattaacc tttgaatctg gagaaatgat tatatctata caagaaatac
     4141 tagatagggt aatgcctaag atattgcctt taataatcac cctaacctcc tttcatctaa
     4201 ttaaaaa
//
LOCUS       DATGSI010000211         1416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_248398, whole genome shotgun sequence.
ACCESSION   DATGSI010000211 DATGSI010000000
VERSION     DATGSI010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248398"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VK031_03245"
     CDS             <1..608
                     /locus_tag="VK031_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52961.1"
                     /translation="RMYTIEMRIRANRVRVYSGASNILRFTANLSGFTGGYAGVRSDG
                     RILSELLRMGDSWTYEPYERFDVVFPDGSKTEYGRLNRTGVTWDNEFQVFTVNSDVEE
                     SATRSEDISMDYDFFHSDLLPLSCGNDYTVKVIPKDINIWLSRLFLGDADGFSILYYQ
                     DVDSLVHWSNEAAYRWKLRGIAIWSLGQEDMRIWEALPKQI"
     gene            693..1100
                     /locus_tag="VK031_03250"
     CDS             693..1100
                     /locus_tag="VK031_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HLS52962.1"
                     /translation="MKEIWNWVQVALTALGGFLGWFLGGFDGFLYALVALMVADYITG
                     VMCAIVDKTLSSEVGFRGIFKKVLIFVLVGIGHIIDTNLIGDGNVLRTAIIFFYCSNE
                     GVSMLENASRLGLPIPEKLKDVLAQLHNKGGNN"
     gene            1100..>1416
                     /locus_tag="VK031_03255"
     CDS             1100..>1416
                     /locus_tag="VK031_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HLS52963.1"
                     /translation="MNLKKLIFTNNACYKAGRKIKPKGIMVHSTGANNPNLRRYVGPD
                     DGLLGKNQYNNHWNQDRPDGRQVCVHAFIGKLKDGSIATYQTLPWDHRGWHAGGDANN
                     THIG"
BASE COUNT          418 a          210 c          330 g          458 t
ORIGIN      
        1 caaggatgta taccatcgaa atgaggatac gagctaatag agttagagtt tattcaggag
       61 caagtaatat cctccgtttc acagcaaatc ttagtggttt tactggaggt tatgctggag
      121 ttcgatctga tggtcgaatt ctttctgaac tattaagaat gggcgattca tggacttatg
      181 agccatatga aagatttgat gtggtatttc cagatgggag caaaactgag tatggaagac
      241 ttaatagaac tggtgtaact tgggataatg agtttcaagt ttttactgtt aatagtgatg
      301 tagaagaatc tgcaacgaga agtgaagata tttctatgga ctacgacttc ttccattcag
      361 atcttttacc ccttagctgt ggaaatgatt atacagtaaa ggttattcca aaggatatca
      421 atatttggtt atcaagactt ttccttggtg atgctgatgg cttttctatt ctctattatc
      481 aagatgtaga tagtcttgtt cactggtcaa acgaagcggc ttataggtgg aaacttagag
      541 gaattgccat ctggtcttta ggtcaagagg atatgaggat ttgggaggca ctccctaaac
      601 aaatataaca ataaatatat atttaggagt atttgaaggt tctgcagatg ctcctttttt
      661 atgtgcaaaa ttatttttag gaggtcgtat gtatgaagga aatttggaac tgggtacaag
      721 ttgctctcac agccctcggt ggctttttag gctggtttct cggaggtttt gatgggtttt
      781 tatatgcttt agttgctttg atggtggctg attatatcac tggggttatg tgtgccattg
      841 tagataaaac tctatccagc gaagttggat ttagagggat atttaagaag gtgctcatct
      901 ttgttttagt tgggattggc catatcatcg atacaaacct tatcggagac ggaaatgtgc
      961 ttaggactgc tattatcttt ttctactgct ctaatgaagg agtttccatg cttgaaaatg
     1021 catcaagact tggacttcca ataccagaga aattaaagga tgtcttagct cagctacaca
     1081 ataaaggggg aaataattaa tgaatttaaa aaaacttatt tttactaata atgcttgtta
     1141 taaggctgga agaaagatta agcctaaggg aattatggtt cattctactg gtgcaaacaa
     1201 tcctaatctt agaaggtatg tgggtccaga tgatggactc cttggtaaaa atcaatataa
     1261 caatcactgg aatcaagata gaccagatgg caggcaagtc tgcgttcatg cttttattgg
     1321 caagcttaag gatgggtcta ttgccactta tcaaaccctg ccttgggatc ataggggatg
     1381 gcatgcaggt ggtgatgcta acaatacgca tatagg
//
LOCUS       DATGSI010000212         2934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_536158, whole genome shotgun sequence.
ACCESSION   DATGSI010000212 DATGSI010000000
VERSION     DATGSI010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536158"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /gene="aspS"
                     /locus_tag="VK031_03260"
     CDS             complement(<1..851)
                     /gene="aspS"
                     /locus_tag="VK031_03260"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HLS52964.1"
                     /translation="MTNKLKRSHYCGAISEANIKEEATVMGWVQRIRDMGGIIFVDLR
                     DKAGILQVVFDVQYVGEKTFNKVEELRNEFVIAVSGKIRPRDEETYNPNIPTGTVELR
                     AENLEILSRSKPLPFPVEDNINVREEVRFTHRYLDLRRPKLYKNLALRHETVKAIRYY
                     LESNDFMEVETPILTKSTPEGARDYLVPSRIHEGKFYALPQSPQIFKQLLMVSGIDKY
                     YQLAKCFRDEDLRADRQPEFTQIDIELSFVDQEDVLNYMESLFKYVFSQVMMIEYKKD
                     FPRISYQ"
     misc_binding    complement(950..1196)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1287..2270)
                     /locus_tag="VK031_03265"
     CDS             complement(1287..2270)
                     /locus_tag="VK031_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HLS52965.1"
                     /translation="MDKKVLMIFTGGTIAMKVDEKLDAAVPGVESNELVNMVKDVNSF
                     TDIETIDFANIPSPHMTPGLMLDLGKLVLKNIVREDITGIVITHGTDTLEETAYILDL
                     MVESEKPIILVGSMRNNSELGYDGAANIWSAIFTACSEEAKGKGVLVVMDDEIHAARY
                     VTKTNTVSTDTFKSPELGPIGVLSNGKTLFYMESIKRQHIDTDTIEEKVALIKAVAGM
                     DGDIIDFYVDKGYKGLVIEALGCGNLPPAMVASVKRAIKKDIPVVLVSRCINGAVEPV
                     YGYEGGGSQLKDMGVIFGGNQSGQKTRIKLMQLLGKTKDINEIREAFLKLV"
     gene            complement(2307..>2934)
                     /locus_tag="VK031_03270"
     CDS             complement(2307..>2934)
                     /locus_tag="VK031_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52966.1"
                     /translation="DLMRQLNLDKIPTIVIESKSYVPNKAESLRENKFLIRANKDYDN
                     IVVAKSLIEAAKDLELAYEYDFDTLDRGGEDFIQASKEFGEKISRLGNKFKKAHRAEE
                     KVNILADLALLVSQDGAGLSFMSNKLQKDVGSAGMLKGTSAVISLLVTEEYIRNYKIP
                     YITETDTKNYKVIIKKAIMLLHQNLEKAEKELREKFDFKPEAYSYLFK"
BASE COUNT          777 a          674 c          425 g         1058 t
ORIGIN      
        1 tcctgatagc ttattctagg gaagtctttc ttatattcaa tcatcattac ctgagagaag
       61 acatatttaa ataaggattc catatagttt aatacatctt cttgatctac aaaggataat
      121 tcaatatcaa tttgggtaaa ttcaggttgc ctgtctgccc ttaagtcttc atccctgaaa
      181 cattttgcca gctgataata tttatctatt cctgatacca tcagtaattg tttaaaaatc
      241 tgtggtgact ggggtagggc ataaaatttc ccttcatgga tcctgctggg aaccagataa
      301 tccctggccc cttctggagt acttttagtt aatatggggg tttctacctc catgaaatca
      361 ttgctttcta aataatatct tatggccttt acagtttcat gccttagggc taggttttta
      421 taaagttttg gccttcgtaa gtccaaatac ctatgggtaa accttacttc ctctcttaca
      481 tttatattat cctccaccgg gaaaggtagt ggtttagatc tggataagat ctctagattc
      541 tctgccctta attctacagt ccctgtggga atattagggt tataggtttc ttcatctcta
      601 ggccttattt tcccacttac agctattaca aactcgtttc ttaactcctc taccttatta
      661 aaggtttttt cacctacata ttggacatca aagaccacct gcaatatacc ggccttatct
      721 cttagatcta caaagattat tcctcccata tctctgattc tttggaccca gcccattacc
      781 gttgcttctt ctttaatatt ggcttcactg atagctccac aataatggct tctttttagc
      841 ttattagtca ttactcttcc tcctatctaa cctttaagtt aagtttgttt acctttactg
      901 gaacaaaatt actggtttgg ataatccaaa taaaaaagcc cttcatccca ctagggacga
      961 aaggctagtt tcgcggtacc acccaagttg gctatctata agataacccc ctcttttata
     1021 tggctatttc catatattct gctgtaatgt agcagctcca tctaagccta ctggccttgg
     1081 ctttcggtta gcgactccca gatgttcttc aattacaatc tctgtgccag tctctcacct
     1141 tacactggct ctctgtgacg aggattataa tctactcttc tgttcatagt ctttttatct
     1201 ttattcaagt taaattatta tatattatca tacagaaaaa tacaatccct gtcaacatat
     1261 tgtatcctaa agcttacctt aatattctaa actaatttaa gaaaggcttc tctaatctca
     1321 ttaatgtctt tagtctttcc cagtagttgc attagcttta tcctggtttt ttggccagat
     1381 tgattaccac caaagataac ccccatgtct tttaattggc tgcctccccc ttcataacca
     1441 tagactggtt ctacagcccc atttatacat ctggatacta gaactactgg aatatctttt
     1501 ttaatggctc tttttacact ggcaaccatg gcaggtggta ggttaccgca gcctaaggcc
     1561 tcaattacta agcccttgta acccttgtcc acataaaaat ctataatgtc tccatccatg
     1621 cctgctacag ccttaattag ggctaccttt tcctctattg tatccgtatc tatatgttgc
     1681 ctttttatgc tttccatata aaataaggtt ttgccattgc ttaatactcc tataggcccc
     1741 agctcaggag acttaaaagt atctgtagat actgtattag tcttggttac atatctggca
     1801 gcatggattt catcatccat taccactaaa acaccctttc ctttagcctc ttcagaacag
     1861 gctgtgaata tggctgacca aatattggcc gccccatcat aacctaattc agagttgttt
     1921 ctcatagagc ctactagaat gataggtttt tcactttcaa ccattagatc taaaatgtag
     1981 gctgtctctt ctaaggtatc agttccatgg gttattacaa ttcctgtaat atcttccctt
     2041 actatatttt taaggaccag cttacccaaa tccagcatta ggccaggtgt catatgtgga
     2101 ctaggtatat tagcaaaatc aattgtttca atatctgtaa agctattaac atcctttacc
     2161 atattaacca gttcattaga ttccacccct ggcactgccg catccagttt ttcatctacc
     2221 ttcattgcaa tggttccacc tgtaaaaatc attaaaacct tcttgtccat ataatcccct
     2281 tccttcactt accttagtta atttccctat ttaaataaat atgaataagc ctcaggctta
     2341 aaatcaaatt tttccctaag ttctttttca gctttctcta aattttggtg caaaagcata
     2401 atagcctttt tgataatcac cttataattt ttagtatctg tctctgtaat ataaggaatc
     2461 ttgtaatttc ttatatattc ttctgtaacc aacaaggata ttactgcaga tgtccctttc
     2521 agcataccgg cgctacctac atctttttgc aacttattgg acataaagct aagacctgcc
     2581 ccgtcctggc taactaagag ggccaagtca gccaatatat ttaccttttc ctctgcccga
     2641 tgggccttct taaatttatt ccctagcctg cttatttttt ccccaaactc ttttgatgcc
     2701 tgaataaagt cctctcctcc tctatccagt gtatcgaagt cgtattcata ggctagttct
     2761 aaatccttgg ccgcctcaat gagggatttg gctactacta tattatcata gtccttgttt
     2821 gctctaatta aaaatttgtt ttcccttagg ctttcggcct tatttggaac ataactttta
     2881 ctttctatta ctattgtagg gattttgtct aaattaagct gcctcattaa atct
//
LOCUS       DATGSI010000213         1000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_583944, whole genome shotgun sequence.
ACCESSION   DATGSI010000213 DATGSI010000000
VERSION     DATGSI010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583944"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VK031_03275"
     CDS             complement(<1..260)
                     /locus_tag="VK031_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52967.1"
                     /translation="MKRLKSCWVCILVLLPLFIFISSFSSSHAPNQPQSKSNIVDKLY
                     VGYFDYSVGGGLYITVKVYVNPSNHSEIISGSDSFSQDFSATG"
     gene            complement(340..>1000)
                     /locus_tag="VK031_03280"
     CDS             complement(340..>1000)
                     /locus_tag="VK031_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52968.1"
                     /translation="KDWIDQYNAEQDAKAKEEQQEYLDANDSHTDEDPAYPYSWTPME
                     ILPDSLYELTIDGKEVSMPFDLTVPANERLAYADLDIYTGRFPKGHNYVLPFTITESS
                     IDISNWKHLGLWFISSPFSGTYTDYSVKSGGLAPDLTGATISLSTIDQHTVEANGFLN
                     YYSGSTTYHFLGDGSVEVTANSGGDLGAEVKESYSDPVTGEFYVKYEAAGAVIEETYK
                     R"
BASE COUNT          320 a          215 c          155 g          310 t
ORIGIN      
        1 ccagtcgcag aaaaatcctg actaaagcta tcgcttccac taattatttc agaatggttt
       61 gacggattaa catatacctt tacggtgata tataatccac ctccaactga ataatcaaaa
      121 tatcccacat aaagtttatc aacaatattc gatttagatt gtggttggtt tggagcatgt
      181 gaacttgaaa aactggaaat aaaaataaat aatggtaata aaaccaaaat gcatacccag
      241 caagatttta accttttcat gatgtataat ttaagtgata aattaataat tgccaaaaac
      301 gcttaaaaac cctacataat ctccttatta cttccctttc tatcttttat aggtttcttc
      361 aataacagct ccagctgctt catatttaac ataaaactct cccgtaacag gatcagaata
      421 gctttctttt acctctgctc ctaagtctcc accgctattt gcagtaactt ctacactacc
      481 atctcccaga aaatgatagg tcgtacttcc actataatag ttcaggaagc catttgcttc
      541 tacagtatgc tgatcaatag tagaaagaga gattgtcgcc cctgttaaat caggggcaag
      601 cccaccactt tttacactat agtcagtata ggttcctgaa aacggtgaag agataaacca
      661 caagcctaaa tgtttccaat tgctaatgtc aatagagctt tctgtaatag taaaaggcaa
      721 cacataatta tgccccttag gaaaccggcc ggtatatata tctaaatcag cgtaagccag
      781 tctttcattt gccggaacgg tcaaatcaaa gggcatactc acctctttac cgtcaattgt
      841 caattcatat aagctatccg gtaaaatttc catgggagtc cagctatatg gataggctgg
      901 atcttcatca gtatgtgagt cattcgcatc taaatattcc tgctgttcct ctttggcttt
      961 ggcatcctgt tcggcattat attggtcaat ccaatcttta
//
LOCUS       DATGSI010000214         1462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_62404, whole genome shotgun sequence.
ACCESSION   DATGSI010000214 DATGSI010000000
VERSION     DATGSI010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62404"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>1462
                     /locus_tag="VK031_03285"
     CDS             18..>1462
                     /locus_tag="VK031_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52969.1"
                     /translation="MHKVLFGDPYQLKEKDILKRKKTSLSPNSTTPISPVYDQNMRKK
                     GNILSDGYELKEGDLGFVEYSDHAETSTVADIVTKSKISSIEVPNTYKNKEQLDHLLK
                     EYGAGKIEVTDGSAYGNMNFFKELMGKQGNWSKEMDILFENDTALARYEGSLENNYTY
                     SDIELRERDREYWSEFNKNGARHISEATLSIIKPIASGAKNNSSFIDAVVDKYSIFPL
                     TYETARKYPDLKKLYYKMNTQRDSGKGYTDYVIFASGRKVGTDTVDPLYNSDGKFNEE
                     PFTSDVHISHDSLCIIAEVAFKGKDKKTLGTQLRNLGPIDLFSNGNPTGNTEEERNRV
                     KEKVDKYQDSHKQLVIQGFNRLLVEWGVDTINPDSVNVDDKYEIRDKGVISELLQSEM
                     LRRLLPDNVKDAVKTYIDEDGNEQFIAPPEATPAYKSIVNIIMSKVDKAFKQPKVSGT
                     DVVQASVGGFSDGITKDENGNLVSDQLKFYEP"
BASE COUNT          560 a          191 c          292 g          419 t
ORIGIN      
        1 ttattgctaa tatagagatg cataaagtat tatttggaga tccttatcaa ttaaaggaaa
       61 aggacattct aaaaagaaag aaaacctctc tatctcccaa tagcacaaca ccaatctctc
      121 ctgtttatga tcaaaatatg aggaaaaaag gaaacatcct atctgatggt tatgaattaa
      181 aagagggaga tctaggtttt gtagagtata gtgatcatgc tgaaacatca acagtagcag
      241 atatagttac taaaagcaaa atatcctcta tcgaagtacc aaatacctac aagaataaag
      301 agcaattaga tcatctttta aaagagtatg gtgcaggaaa aattgaggtt actgatggtt
      361 ctgcttatgg aaacatgaac ttctttaaag aactaatggg taaacaagga aattggagca
      421 aagagatgga tatactattt gagaatgata cagccttggc aagatatgaa gggagtttag
      481 agaataatta tacttatagt gatatagaac ttagagaaag agatagagaa tactggagtg
      541 aatttaataa aaatggagcc agacatatct cagaggctac attaagtatt attaaaccaa
      601 tagcatctgg tgctaaaaat aattcgtctt ttattgatgc tgttgtggat aaatattcca
      661 ttttcccatt aacttatgaa acagccagga aatatcctga tcttaaaaag ctgtattaca
      721 aaatgaatac ccaaagagat agtggaaagg ggtacacaga ctatgtaata tttgcttcag
      781 gaagaaaagt agggacggat acagtagatc ctctatataa tagtgatggt aagtttaatg
      841 aggagccttt tacttctgat gtgcatatat ctcatgattc cttatgtatt attgccgagg
      901 tagcattcaa agggaaagat aaaaagactt tgggtacaca gttaagaaat cttggtccta
      961 ttgatttgtt ctctaatggt aaccctacag gtaatacaga agaggaaaga aacagggtga
     1021 aagaaaaggt tgacaagtat caagattcac ataaacaatt agtcatacaa ggatttaata
     1081 gattattagt agagtggggg gtagatacta taaaccctga cagtgtaaat gttgatgata
     1141 aatatgaaat aagagacaaa ggagtaatat ctgaattact tcaatctgaa atgttgagga
     1201 gattattacc cgataatgtt aaagatgcag taaaaactta tatagatgaa gatggtaatg
     1261 aacaatttat tgctccacct gaggctacgc cggcttacaa atcaatagta aatattatta
     1321 tgtctaaggt tgataaagca tttaaacaac ctaaagtaag tggtactgat gtagttcaag
     1381 cctctgtagg agggttttct gatggaatta ctaaagatga aaatggtaat ttagtttctg
     1441 accaactaaa gttctatgaa cc
//
LOCUS       DATGSI010000215         1333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_297193, whole genome shotgun sequence.
ACCESSION   DATGSI010000215 DATGSI010000000
VERSION     DATGSI010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297193"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VK031_03290"
     CDS             complement(<1..505)
                     /locus_tag="VK031_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HLS52970.1"
                     /translation="MKLRVKFNKKNYLKYISHLDLIRLFQRSFNRAQIPVKYSEGFNP
                     QPRFSIAHPLSLGIESCEEYMDIELEEKIDPLDFMERINQILPKDIQILDCIYPKDKR
                     PIAAIISLAHYEISFGLVEDMDEVKGQIDRWLEKEKIIITRIRKRRGRQIKREQNIRP
                     LIKKLAFT"
     gene            complement(502..>1333)
                     /locus_tag="VK031_03295"
     CDS             complement(502..>1333)
                     /locus_tag="VK031_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLS52971.1"
                     /translation="GVSLPSLRVDSVSIDILKEIERVRKTGLTFAPEAGSQRLRNVIN
                     KGVSEEDLTTAVSYAFKEGWSRIKLYFMIGLPTETDEDVLGIKDLAYKVKDLFFNRPR
                     EEKKGSFKLTVSASCFIPKPFTPFQWVGQNSIEEFKRKAYLINDNIRDRRISFNYHSP
                     EVSYIEAIISRGDRRVGRAIYKAWQLGCRFDGWSEHFDFDKWLQALDEANIDGDFYAL
                     RNRSFEEILPWDFIDPGVSKKYLFREFKKSLKEIVTRDCRLGCTACGIKDCAMRGVFN
                     "
BASE COUNT          340 a          293 c          221 g          479 t
ORIGIN      
        1 ttgtaaaggc cagtttcttt atcagtggcc ttatattttg ctcccttttt atctgtcttc
       61 ctcgcctttt tctaatcctg gttataataa ttttttcctt ttctagccac ctatctattt
      121 ggccctttac ctcatccata tcttccacta ggccaaagct tatttcataa tgggctagac
      181 ttataatggc tgctataggc cttttatcct taggataaat acagtctagg atttgtatat
      241 ctttgggtaa gatttggttt atcctttcca taaagtctaa aggatctatt ttttcttcta
      301 attctatgtc catatattct tcacaacttt caatccctaa ggatagggga tgggctatgg
      361 agaacctagg ttggggatta aagccttctg aatatttaac aggtatttgg gccctattaa
      421 agcttctttg aaagagcctt attagatcca ggtgggatat atattttaaa tagtttttct
      481 tattaaattt cacccttagt ttcaattaaa tacacctctc atggcacagt ctttgattcc
      541 gcaggctgta caacctagcc tacagtctct agttactatt tcttttaagg actttttaaa
      601 ctccctaaat aaatattttt tgctaactcc tggatctata aagtcccaag gtagaatctc
      661 ttcaaaggat ctatttctta aggcataaaa atctccgtct atatttgctt catctaaggc
      721 ctgtagccat ttatcaaagt caaagtgttc agaccaacca tcaaacctac agcctaactg
      781 ccaggccttg taaattgccc ggcctaccct tctgtctccc ctggagatta tagcctctat
      841 atagctaacc tctgggctat ggtagttgaa gctaatccgc ctgtccctta tattatcatt
      901 gattaagtag gcctttcttt taaattcttc aatggaattt tgtcctaccc attggaaggg
      961 ggtaaagggt tttgggataa agcaggaggc actaactgtt agcttgaagc tacccttttt
     1021 ttcctccctg ggcctgttga agaaaagatc cttcacctta taggccaggt ctttaatacc
     1081 tagtacatct tcatcagttt ctgtaggtag cccaatcata aagtaaagtt taatcctaga
     1141 ccagccctcc ttaaaggcgt aggatacggc tgtagttaaa tcttcttcac tgaccccttt
     1201 attgattaca tttctcaacc tttggctgcc tgcctctggt gcaaaggtta ggccagtttt
     1261 tctaaccctt tctatttcct ttaatatatc tatgcttaca gaatctaccc ttagggaagg
     1321 tagggatact cct
//
LOCUS       DATGSI010000216         4091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_248624, whole genome shotgun sequence.
ACCESSION   DATGSI010000216 DATGSI010000000
VERSION     DATGSI010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248624"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VK031_03300"
     CDS             <1..209
                     /locus_tag="VK031_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pantothenate ECF transporter"
                     /protein_id="HLS52972.1"
                     /translation="GTLTNTVGVLLMIYLLYGERFVLSQGLNPLHARKIILGIAFTNG
                     IPEAIIAIIVVVSVVNILQAKESE"
     gene            212..994
                     /locus_tag="VK031_03305"
     CDS             212..994
                     /locus_tag="VK031_03305"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HLS52973.1"
                     /translation="MEDLLLVIDVGNTNIVFGVYQGKSLLYNWRIATEKNRTSDEYGL
                     LFEQIFRYHKLNIRSVTDIIISSVVPPLMNTLAVMGKKYFGLEPIIVGPGIRTGINIK
                     YDNPKEVGADRIVNAVGGYNKYRGPLILVDFGTAITFDAISETGDYLGGAITPGITIS
                     MEALFMRTAKLPKVEVERPDNVIGKNTVESIQSGIVYGYVGLVDYIIEKMAKEMADDV
                     DKVKVVGTGGFAQLIAKESKYIKKTDKLLTLEGLKIIYERNR"
     gene            1024..1986
                     /gene="dusB"
                     /locus_tag="VK031_03310"
     CDS             1024..1986
                     /gene="dusB"
                     /locus_tag="VK031_03310"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HLS52974.1"
                     /translation="MVKIKIGNIDIKGKAFLAPMAGVTDMTFRRICKIMGAGLVVTEM
                     VSAKSLYYNDKKAKSLTKAHKDESPIALQIFGTDAELLAKVIKERINSRKDIDIIDIN
                     MGCPVPKLVKKGAGSALMREPKKVRYILNQLVQVSEKPITVKIRIGWDKASLNAVEIA
                     KIAEEAGVDGITVHGRTRDMLYSGRADWTYIAKIKESVSIPVVGNGDVFTPDDGIRLL
                     KETGCDAVAIGRGALGNPWIFKGINSILEGKSYYQPTEREIIDMAIKHLNMACRDKGE
                     RIAIRQMKKHLLAYVDRLEAGDRIRARVSRGESKREIENIFKAY"
     gene            2065..2976
                     /locus_tag="VK031_03315"
     CDS             2065..2976
                     /locus_tag="VK031_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52975.1"
                     /translation="MIYVLDYRNYKKELEDGFYKYLFKFVTPMFMPKKIDTMCFNKDK
                     RVHIVGINLRKVNYQEEEILNEFISKLLKIKSQNQGYLYLEDMDGMDKKILKVIEKET
                     GMKIPTGLDIKIFNIPLILEEISNLSKRNIRDSEVLIIGENKDQLLNTINQVAHLLRF
                     ISLIGIDQAGQDEIYQEVLETTGLSILQPKKYESDLKKYDVIINLSDKQLVDVSRIKR
                     ESIVFDLSLGKNLTSIKNPLVISDISIACKGETVGSGHPIANHISSGPYQFISNGRKE
                     KFHRVYIKDRYYDLKEFFKINRPKGTF"
     gene            3121..3597
                     /gene="greA"
                     /locus_tag="VK031_03320"
     CDS             3121..3597
                     /gene="greA"
                     /locus_tag="VK031_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HLS52976.1"
                     /translation="MAEKQVFLTMEGLKKLEEELDELKSVQRKEVAERIKQALSFGDI
                     SENSEYDQAKNDQAQLEERIIKLEMILRNARIIDEDEITTDIVSIGSKVLVKDLEYDE
                     EMEYLIVGSAEADPYDGKISNESPLGSALLGRKKGDVVEVEVPDGTISYEILDITR"
     gene            3627..>4091
                     /locus_tag="VK031_03325"
     CDS             3627..>4091
                     /locus_tag="VK031_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HLS52977.1"
                     /translation="MSEKPQDLNEMLMIKRQKLEELIEKGKDPFLIEEYDVTHKSKEI
                     KENFEELEEERVAVAGRIMSRRGHGKVSFIDIQDSEGRIQIFVQFDQVGEETYKDLSL
                     LDIGDIIGVSGEVFRTRAGEISIRAKELTLLSKSLQILPEEFHGLKDQDLRYR"
BASE COUNT         1565 a          494 c          915 g         1117 t
ORIGIN      
        1 caggaaccct taccaatact gttggagttc ttttgatgat ctatttattg tatggagaaa
       61 ggtttgtttt aagccaggga ttgaaccctt tacatgctag aaagattatt ttaggaatag
      121 cttttaccaa tggaattcca gaggctataa tagctataat tgtggttgta agtgtagtta
      181 atatattaca agctaaggaa agtgaataga tatggaagat ttattattag taatagatgt
      241 gggaaacact aatatagtgt ttggagttta tcaagggaaa agtctacttt ataattggag
      301 aatagctaca gaaaaaaata gaacgtcaga tgaatatgga ctattgtttg agcagatatt
      361 tagatatcat aagctgaata taaggtctgt aactgatata attatttcct cagtggttcc
      421 gccattaatg aataccttgg cagtcatggg gaaaaaatat tttggattag aaccaattat
      481 tgttggacca ggtataagga ctgggataaa tattaaatac gataacccta aagaggtagg
      541 ggccgatagg attgtaaatg ctgtaggtgg ttacaacaaa tataggggac ctttaatatt
      601 agttgacttt ggtactgcca ttaccttcga tgccatatca gagacaggag actatctggg
      661 aggggccata acaccaggta ttaccatatc tatggaggcc ctatttatga gaacagccaa
      721 gttgccaaag gtggaagttg aaagaccaga caatgtaatt ggaaaaaata ctgtagaaag
      781 tatccaatcg ggaatagtat acggatatgt aggactggta gactatatta tagaaaagat
      841 ggccaaggaa atggcagatg atgtagacaa ggtaaaggtt gttgggactg gtggatttgc
      901 ccaactgatt gccaaggaaa gtaaatatat aaagaagaca gataaacttt taaccttaga
      961 aggtttaaaa attatatatg agaggaatag gtaagtagct agctacttac ctattatttt
     1021 gaggtggtta agataaaaat agggaatata gatataaaag gtaaagcatt tttagcgcct
     1081 atggccggtg taactgatat gacctttaga aggatttgta agataatggg ggctggcctt
     1141 gtggttacag aaatggtaag tgcaaagagc ctatattata atgataaaaa agctaaaagc
     1201 ttaactaagg cccacaagga tgaatcacct atagccctac aaatatttgg aacagatgca
     1261 gaacttttgg ccaaggttat aaaagaaagg attaattcta gaaaagatat agatattata
     1321 gatattaata tgggttgccc tgtacccaag ctggtgaaaa aaggtgctgg cagcgccttg
     1381 atgagggaac ctaaaaaagt aaggtatata ttaaatcagt tggttcaggt atcggaaaaa
     1441 cctattactg taaagattag aataggctgg gataaagcta gtctaaatgc tgtagaaata
     1501 gccaaaatag ctgaagaggc aggagtagat gggataacag ttcatggaag gactagggat
     1561 atgctatatt cgggaagggc agattggaca tatattgcta aaataaagga aagtgtatcc
     1621 ataccagttg ttggtaatgg agatgtattt actccagatg atgggattag acttttgaaa
     1681 gaaactggct gtgacgctgt agctataggc agaggtgcct taggaaatcc ttggattttt
     1741 aaagggatta atagtatttt agaaggaaaa agttattatc agcctacaga aagagagatt
     1801 attgatatgg ccataaagca tttaaatatg gcttgtaggg acaagggaga aaggatagct
     1861 ataagacaga tgaaaaagca cctacttgca tatgtagata gattggaagc tggagacaga
     1921 attagagcaa gggtaagccg gggagaaagt aagagagaaa tagaaaatat atttaaagct
     1981 tactaaataa gactttttct taatgaataa gaagtagttc cctatcatat aatggtaaaa
     2041 cattatgcta gggatgtgat ctgtttgata tatgtattag attacaggaa ctataagaaa
     2101 gaactggagg atggatttta taaatattta tttaaatttg tcacccccat gtttatgcct
     2161 aaaaaaatag ataccatgtg ttttaataag gacaaaaggg tccatatagt aggtataaat
     2221 ttaaggaagg taaattatca agaagaggaa attctaaatg agtttatctc taagctttta
     2281 aaaataaaat ctcaaaatca aggttacctg tacttggaag atatggatgg tatggacaag
     2341 aaaatattaa aggtaataga aaaagagacc gggatgaaaa tacctacagg cctagatata
     2401 aaaatattta atatacccct aatattggag gaaatatcta atctatctaa aagaaatata
     2461 agggatagtg aagtgttaat tataggtgaa aacaaggatc agcttctaaa tacaataaac
     2521 caggttgctc atctattaag atttatttcc ttaataggta tagatcaggc tggccaagat
     2581 gaaatttacc aggaggtttt ggaaaccacc gggctatcta ttttgcaacc taaaaaatac
     2641 gaaagtgatt tgaaaaaata tgacgtgatt attaatcttt ctgacaagca actagtcgat
     2701 gtaagcagga taaagagaga gtcaatagta tttgaccttt ccttaggtaa aaatctaaca
     2761 agcattaaaa atcctttagt gatttcagat atcagtatag cctgtaaagg ggaaactgta
     2821 ggctcaggcc atcctatagc taatcatatt tcctctggcc catatcaatt tatatcaaat
     2881 ggaagaaagg aaaaattcca tcgagtatat attaaagaca ggtattatga tttaaaggaa
     2941 ttttttaaaa tcaacaggcc taaaggcact ttttaaatac ttgacatcgg cctatacctt
     3001 gattataata ttttccatat aatatggcta gtaaattatt ttttgacata aagttaaaat
     3061 aaaagattta aaagaaattc aggctttgcc tagagggtat aatgaaaggg agagagttta
     3121 atggctgaaa agcaagtatt cctaactatg gaaggactaa aaaaattaga agaagaatta
     3181 gatgaattaa aatcggtaca gagaaaagaa gtggcagaaa gaatcaaaca ggcattgagt
     3241 tttggagaca ttagcgaaaa ttctgagtac gatcaggcta aaaacgacca ggcccaattg
     3301 gaagagagaa taatcaaact tgaaatgata ttgagaaatg ctagaataat agatgaagat
     3361 gaaataacaa cagatatagt aagtataggg tcaaaggttt tggtaaaaga cttagaatat
     3421 gatgaagaaa tggagtactt aatagtaggg tcagcagagg ccgatcctta tgatggaaaa
     3481 atatcaaatg aatcaccgct aggaagtgcc ctattagggc gtaaaaaggg agatgtagta
     3541 gaagtggaag tgccagatgg aactataagc tatgaaatcc ttgatataac cagataaggg
     3601 acggggcaaa ctaaggagga ttaaaaatgt cggaaaaacc acaagattta aatgaaatgt
     3661 taatgattaa aagacagaag ttggaggaac ttatagaaaa aggtaaggat cccttcttaa
     3721 tagaagagta tgatgttacc cataaaagca aggaaataaa agaaaatttt gaggaacttg
     3781 aagaagaaag ggtagctgtg gccggtagga taatgtctag aagaggccat ggaaaggtta
     3841 gttttattga tattcaagac agtgaaggca ggatccagat ctttgtacaa tttgatcaag
     3901 taggtgaaga aacatataag gacctatcct tattagatat aggagatata ataggggtta
     3961 gtggagaagt atttaggact agagcaggag aaatatccat cagggcaaaa gagcttacac
     4021 tactaagtaa atcattacag atattaccag aagaattcca cggcctaaag gatcaagatt
     4081 taagatatag g
//
LOCUS       DATGSI010000217         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_112255, whole genome shotgun sequence.
ACCESSION   DATGSI010000217 DATGSI010000000
VERSION     DATGSI010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112255"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..423
                     /locus_tag="VK031_03330"
     CDS             160..423
                     /locus_tag="VK031_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HLS52978.1"
                     /translation="MYKENITIQNETGLHARPASLFVKEASKYSSDIKVIKDGQEYNA
                     KSIMGILSMGAAKGDVLTIQAEGADAEEAVKNLVDLVENKLED"
     gene            436..2142
                     /gene="ptsP"
                     /locus_tag="VK031_03335"
     CDS             436..2142
                     /gene="ptsP"
                     /locus_tag="VK031_03335"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HLS52979.1"
                     /translation="MKGLGTAPGIGIGKAFVFKNPEIIVEKKQVEDLDKEIKRLKEAV
                     KIAGNQIDTIYESAIDEIGEEEAEIFSAHKMMLEDPEFLEDIEQRITNEKINAEWAVN
                     ETANKMINLFENMEAEYFRERAADLRDVSNRLLRVLLGIETADLMAINQESILIAEDL
                     TPSDTAEMNKRMVVGIVTDLGGRTSHTSIIARTLGIPAIAGIKNADEVINHGDLMIID
                     GDTGNIIVNPSQEELAKYRKKQEEINKFRAKMQEMIGKETVSKDGHKVELAGNIGSVE
                     DLEQVLKNDGEGVGLFRTEFLYMDNDRLPTEEEQFVVYKEVAEKLEGKPLVIRTLDVG
                     GDKEIPYLELPKELNPFLGYRAIRLCLDRPDIFKTQLRAIYRASNYGNIKIMFPMISN
                     MDELRAAKAIAQEVREELKNENIPFSQDVEIGIMVEIPAVAIHSEVFAKEVDFFSIGT
                     NDLIQYTVAVDRGNQQIEHLYNQYHPSVLKLIQMTIENGHKAGIWVGMCGEVAGDEKL
                     IPVLLAMGLDEFSMNPGSILRARYQIRNSSKKEVEKQLDTLLGLATAQEVEDFIDEKI
                     LK"
     gene            2231..2953
                     /locus_tag="VK031_03340"
     CDS             2231..2953
                     /locus_tag="VK031_03340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HLS52980.1"
                     /translation="MYIRLFIIAITPGIIGLFSIYLTDRVDREPAKLLFITFILGALA
                     VIPSIIVEELLVRLNVFSGVYGAFFNAYIVAGLTEEYFKRLVVLKVPFRTSYFNEKLD
                     GIVYCVYSALGFATVENIIYVVFRYTNNPFIGLYRGIFSVPAHGIFGVTMGYYMSLSK
                     FNTDEQGKVSNMKKSLIMPIILHGTFDFILMANIPELSILFVPYVVFLWWLNQRKLEA
                     FVFDSRSRISHDSSENINRREE"
BASE COUNT         1221 a          427 c          676 g          874 t
ORIGIN      
        1 ctatattatt gggggaaaat gttttcagaa caattagaaa atcaatataa gtaatcaaaa
       61 ttatatttaa aataaattat ataaagcaaa ggattgtgtt aatatagtaa tatgtttata
      121 aaataaaata aatttattat aaaatgtagg aggagaaaga tgtataagga aaatattact
      181 attcaaaatg aaactggtct tcatgccaga cctgcatctt tatttgtaaa ggaggcatcc
      241 aaatattcat cagatattaa agtaataaaa gatggacaag agtataatgc caagagtatt
      301 atgggtatat taagtatggg agcagctaag ggagatgtat taactattca agccgaggga
      361 gcagacgcag aggaagcggt gaaaaactta gttgacttgg tagagaataa gctagaagac
      421 taggaggagg atttaatgaa aggcttagga acagccccag gaattgggat tggaaaagct
      481 tttgttttca aaaacccaga aataatagta gaaaaaaagc aagtagaaga cctagataaa
      541 gaaataaaaa gacttaaaga agctgtaaaa atagcaggaa atcagataga cacaatatat
      601 gaatcagcta tagatgaaat tggggaagaa gaggcagaaa tattttcagc acacaagatg
      661 atgttagaag atcctgaatt tttggaggat atagagcaaa gaatcaccaa tgaaaagatt
      721 aatgcagaat gggcggtaaa tgaaacagct aataagatga taaatttatt tgaaaatatg
      781 gaagcagaat atttcaggga aagggcagca gacttaagag acgtatcaaa cagactactg
      841 agagtcctac tggggataga gactgcagat ctgatggcca tcaatcaaga atccatctta
      901 attgcagaag atttaacgcc ttctgataca gctgaaatga ataaaagaat ggttgtaggt
      961 atagtaacag acctaggtgg tagaacatct catacctcaa tcatagccag aacactgggt
     1021 atacctgcca ttgctggaat aaagaatgca gatgaagtta taaaccatgg agatttaatg
     1081 attatcgatg gagatacagg aaatataata gttaatccta gccaagaaga attggccaaa
     1141 tataggaaga aacaagaaga gataaataaa tttagggcca agatgcagga aatgattgga
     1201 aaagaaactg tatccaaaga tggccacaaa gtagagttgg ctggaaatat tggatctgta
     1261 gaagacttgg agcaggttct taaaaatgat ggggagggtg taggtctatt tagaacagaa
     1321 ttcctatata tggataatga tagattgcct acagaagaag aacaatttgt agtatacaag
     1381 gaagtagctg aaaaacttga aggaaagcct ttggtaataa ggaccttaga tgtaggcgga
     1441 gataaggaaa tcccttattt agaactgcca aaagaattaa acccattcct aggctatagg
     1501 gccataagac tatgtctaga caggccagat atattcaaga cccagctaag ggccatatac
     1561 agggccagta attatggcaa tataaaaatc atgtttccaa tgatatctaa catggatgaa
     1621 ctaagggcag ccaaggccat agcccaagaa gtaagggaag agttaaagaa cgaaaatatt
     1681 cccttcagcc aggatgtaga gataggtata atggttgaaa taccggcagt agccatccat
     1741 tctgaagtgt ttgctaagga ggtagacttc ttcagcatag gaaccaacga cctaatccaa
     1801 tatacagttg cagtagatag gggtaaccaa cagattgaac atttatacaa tcaataccat
     1861 ccttcagtat taaaattaat acaaatgacc atagaaaatg gccataaggc tggtatatgg
     1921 gtaggaatgt gtggggaagt agcaggggat gaaaagttaa tcccggtgct attggccatg
     1981 ggattggacg aatttagtat gaatccaggc tccatcctaa gagcaaggta tcagataaga
     2041 aactcttcta aaaaagaagt ggaaaaacaa ttagataccc tattaggatt agccactgca
     2101 caggaagtgg aagactttat agatgaaaaa atattaaaat aaatttttaa taaaccctaa
     2161 gaagattctt agggtttatt tttatcctgt aatggcataa taaatactaa ccaccaaagg
     2221 aggaaaagac ttgtacatca gattatttat tatagccatt acaccaggaa taataggctt
     2281 gttttccata tatttaactg atagggtaga tagggagcct gccaaactat tatttataac
     2341 ctttatctta ggagccttgg cagtaatccc atccataata gtggaagaat tgttggtaag
     2401 attaaatgta ttttctggag tctatggggc attttttaat gcctatatag tagcagggtt
     2461 gacagaagaa tattttaaac gtttagtcgt tttaaaggtt ccttttagaa ccagctactt
     2521 taatgaaaaa ttagatggaa ttgtatattg tgtatattca gccctaggtt ttgccacagt
     2581 agaaaatata atttatgtgg tctttaggta tactaataat cccttcatag gcctatatag
     2641 ggggatcttt tcagttccgg cccatggaat ttttggagta actatgggtt attatatgtc
     2701 tttgtctaaa tttaacacag atgagcaagg aaaggtatct aatatgaaaa aatcacttat
     2761 tatgcctatc atccttcatg gtacctttga ttttatactg atggccaata taccagagtt
     2821 aagcatactc tttgtcccct atgtagtatt tttatggtgg cttaaccaaa ggaagttgga
     2881 agcctttgta tttgactcta gaagcaggat ttcacatgat agttctgaaa atatcaatag
     2941 aagggaagaa taattatccc ttctattgaa cttaggctaa ggaaggtata gaatacatat
     3001 aagaaaaatt tggacatgca ttaattagtt aggagtagat ataatgaata gaaatcctag
     3061 aatggaaaga aatagaagga gaaaattaag gcgaaaaagg ataatcataa atagaataat
     3121 tttgctttta ataataattt cactagcctt tatcttggtt aagggcaagg gaaaaataca
     3181 gaatttaact tataatat
//
LOCUS       DATGSI010000218         4342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_206785, whole genome shotgun sequence.
ACCESSION   DATGSI010000218 DATGSI010000000
VERSION     DATGSI010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206785"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     misc_binding    117..353
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            462..1430
                     /locus_tag="VK031_03345"
     CDS             462..1430
                     /locus_tag="VK031_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLS52981.1"
                     /translation="MKKGRFSKLIVLVILSMLVLTACDSAGKNQFTIGISQFAEHSAL
                     DDARLGFEEGLKELGIDAKIVYQNAQADVATTNTIAEKFVKDKVDLIYAIATPAAQSA
                     KRVTEDIPVLFSAITDPVDAELVESMEAPGGNITGTSDEAPMKEQLSLFKKIDENIET
                     IGIIYNTGEPNSEIQINKAHKLAPNLGLEIFPMGISNINDMPQAMDSLVKKVDAIYTI
                     TDNMVASAINIIAEKAIENKMITVGAEDAHVAGGILISDSLSYFELGKQTAHMAKEIL
                     IDGKSPAHIPAERAENTKLIFNNETLKSLGLDGNKQVFKEAKKLSN"
     gene            1449..2336
                     /locus_tag="VK031_03350"
     CDS             1449..2336
                     /locus_tag="VK031_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLS52982.1"
                     /translation="MNSLIITSIEQGLIFAVLAMGVFITYKILDIADLSVEGSFPFGA
                     FVFAKLVSIGFHPVPATITAFFVGTLAGLLTAMLFTKVKIKPILSGILTMTILYSVNL
                     RTNGKANVPLFNYGSIFDLGPKLVILTLIVLLIKICLDLFLKTETGYLLIATGDNEAL
                     VRSLGTDSNKYKLIGLMIANGLVALSGALMAQYQGFADMTMGNSIIVIALASIIIGDT
                     IKRESNLIKNTTRAIVGAIIYKIIGGVAIDLGLNPQDLRAVNALIVIIFISYNNFAVD
                     FFKSFKKGGQKLARNSQHV"
     gene            2311..3078
                     /locus_tag="VK031_03355"
     CDS             2311..3078
                     /locus_tag="VK031_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS52983.1"
                     /translation="MLEIVNMSKSFNKDSENEIKVFEDFNIKIAANKCTSIIGANGCG
                     KSTLLNIIAGSVPSDNGKIILKGRNIEQLREEERALHMGRVYQDPSMGVSPSLTILEN
                     LSLADKKGEKFTLRRLVKRDKIHKYIELLKDLDLGLENKLNTKVKYLSGGQRQSLSLI
                     MATMKHPDLLLLDEHTAALDPKTSHVVMEKTRELINKYKITTIMISHNMKDAIDYSDR
                     IIMLDRGKVVLDKASKDVTEKELIDIYRAKLMDEIAS"
     gene            complement(3108..3302)
                     /locus_tag="VK031_03360"
     CDS             complement(3108..3302)
                     /locus_tag="VK031_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52984.1"
                     /translation="MDRAAVVGGDLSAKQVLENDLIKSTKAYENDRIIYLDAHVWYVS
                     SGGLTGTMKMVEEIEKALDN"
     gene            complement(3312..4055)
                     /locus_tag="VK031_03365"
     CDS             complement(3312..4055)
                     /locus_tag="VK031_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLS52985.1"
                     /translation="MKNIRKSILFVGLIILSLIFVSCDFQPSDTGKENPEEMITIKHE
                     LGEEKVKKNPERIIVFDYGILDALDNIGIDVIGVAKQSLPAYLDKYKADEYVDIGSLK
                     EPNFETIYEINPGLIIISGRQGDIYDELRKIAPTVYLNIDGGDYLNSFKSNMEILGKM
                     FDREDLLKEKVEEITEAIDKLHEKAQSIDKNGLFIMANDGNLSAYGLGSRFGVLHKEF
                     GVKAADENIDASTHGQKITFEYIVETDPD"
     gene            complement(4080..>4342)
                     /locus_tag="VK031_03370"
     CDS             complement(4080..>4342)
                     /locus_tag="VK031_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="siderophore ABC transporter ATP-binding protein"
                     /protein_id="HLS52986.1"
                     /translation="LDMKHSVEIMKILRSLVDDLGKTIAIVIHDINFASCYSDYIVAL
                     KDGKIAEVGNTDKIINKNSLGRIFDMDFTVKDIENMRLCVYY"
BASE COUNT         1433 a          782 c          732 g         1395 t
ORIGIN      
        1 ctctcctttt taacagtcta gatagataaa ttattctata taataattaa gaccagttaa
       61 tttcaaaaaa tacttgacat tatcacagta aagcattatt atatagttta aataatttaa
      121 atgctatgat ggagagaaag atatcactag ttatttacag agagttgggt caggtgaaag
      181 ccaacaataa caggatatgt aatatacact cctaagcaga tttgcttaaa ggtaacaagt
      241 aggcaaatcc gaaggcccat cgttacaagg gataggactg ttaggtctga ttgaagtgat
      301 ggctttttgc cataactagg gtggtaccgc ggatataatc tttcgtccct attttgtagt
      361 cttaactaca gataggaatg aaagattttt ttattaccct ttttccatgg aattcaatca
      421 agctaattat cctaaatatt aaattcaggg aggataatag gatgaaaaaa ggaaggttta
      481 gtaaattaat tgtattggtt attttaagta tgttagtatt aactgcttgt gattcagctg
      541 gcaaaaatca atttaccatt ggaatatccc agtttgcaga gcattctgcc ttagatgatg
      601 ctagattagg ttttgaagaa ggcctcaagg aactaggtat agatgctaaa attgtttacc
      661 aaaatgctca agctgatgta gccactacca atactattgc tgaaaagttt gttaaagata
      721 aggtagattt aatctatgct attgcaactc cagcagccca gtcagccaaa agagttactg
      781 aagatatacc agttctattt agtgccataa cagatcctgt tgatgcagaa ttggttgaaa
      841 gtatggaggc tcccggtggc aatataactg gaacctctga tgaagcacct atgaaggaac
      901 agctatcttt gtttaaaaag atagatgaga atatagaaac tatagggatt atctataata
      961 caggtgaacc taattctgag atacaaataa ataaggctca caagctggct ccaaacctgg
     1021 gcctagaaat atttcccatg ggaatttcta atataaatga tatgccccag gctatggatt
     1081 ccttagtaaa gaaagtagat gccatctata ctattacaga taatatggta gcatcagcca
     1141 taaatataat tgcagaaaag gccatagaaa acaaaatgat tactgttgga gccgaagatg
     1201 cccatgtggc aggtggcata cttatttccg atagtttaag ttattttgaa cttggaaaac
     1261 aaactgccca tatggccaag gagattttaa ttgatggcaa gtctccagct catattcctg
     1321 ctgaaagggc agaaaatact aaattaatat ttaataatga aactttaaaa tccttagggc
     1381 ttgatggaaa taagcaagtt tttaaggagg ctaagaagtt atccaattaa taaatggagg
     1441 tctaggtaat gaattcttta attataacat ctatagaaca ggggctgatt tttgcagtcc
     1501 tggctatggg cgtatttatc acatacaaaa tattagatat agcagattta tcagtagagg
     1561 gtagtttccc ctttggcgcc tttgtctttg ctaaattagt atccataggc tttcacccag
     1621 taccagctac tattactgcc ttctttgtag gaacattggc aggattactg acagctatgc
     1681 tttttaccaa ggttaaaata aaacctattt tgtccggtat ccttaccatg accatcctat
     1741 attcagttaa ccttaggacc aatggtaagg caaatgtacc cttgtttaac tatggttcta
     1801 tttttgactt aggtcctaaa ctggtgattt tgacccttat tgtacttttg atcaaaatct
     1861 gcctagacct atttttaaaa actgaaaccg gctatctgct tatagccact ggcgataacg
     1921 aggccttagt tagatcccta ggtactgaca gtaataaata taaattaata ggccttatga
     1981 ttgccaacgg gctggtagct ttaagtggcg ccttaatggc ccagtatcag ggatttgcag
     2041 atatgaccat ggggaattcc ataattgtta ttgccctggc ctccattatc ataggtgata
     2101 ctattaaaag ggaatcaaac cttattaaaa ataccactag agctattgtt ggagccatta
     2161 tctataagat aataggaggc gttgcaatag atttaggcct taatcctcaa gatctaaggg
     2221 cagtcaatgc cttaattgtg ataattttta tttcctacaa caattttgcg gtggattttt
     2281 tcaaatcctt taaaaaagga ggtcaaaaac ttgctagaaa tagtcaacat gtctaagagt
     2341 ttcaacaagg atagtgaaaa tgaaattaag gtctttgaag attttaatat aaaaatagcg
     2401 gcaaacaaat gcacctccat tatcggtgcc aatggatgtg gtaaaagtac tcttttaaat
     2461 ataatcgctg gtagcgtccc atctgataat gggaaaataa tattaaaggg cagaaacata
     2521 gaacagttga gagaggaaga aagagccctt catatgggta gggtatatca ggacccatcc
     2581 atgggcgtat ccccctccct taccatacta gaaaacctat ccctggccga caaaaaaggt
     2641 gaaaaattca ctttaagaag gctggttaaa agggataaaa tccataaata tatagaactc
     2701 ttaaaagact tagatctagg tcttgaaaac aaacttaata ctaaggttaa gtatttatca
     2761 ggtggccaaa ggcaatcctt gtctttgatt atggctacca tgaagcatcc agatcttcta
     2821 cttttagatg agcatacggc tgccctagat ccaaaaacat cccatgtggt catggaaaaa
     2881 accagggaat taataaataa gtataagatt acaaccataa tgatctctca caatatgaag
     2941 gacgccattg attattcaga caggattatt atgctagata gaggtaaagt tgtattagat
     3001 aaggcaagta aagatgtaac agaaaaagag ctaattgata tatacagggc caagctaatg
     3061 gatgaaattg cttcctaaat aaaaactcag ggcaaatagc cctgagttta attatctaaa
     3121 gctttttcta tttcttctac cattttcata gtaccagtta agccaccgga ggatacatac
     3181 catacatgtg catctaaata aattatccta tcattttcat aggctttggt tgatttaatc
     3241 aaatcatttt ctaaaacctg ctttgcagat agatctcccc ctaccacagc agccctatcc
     3301 attacaaata actaatctgg atctgtttct actatatatt cgaaggtaat cttttgtcca
     3361 tgggtgctgg catcaatatt ttcatcagcc gccttcactc caaactcttt gtgcagaact
     3421 ccaaatctag atcccagtcc ataggcactt aagtttccat cattggccat aataaataat
     3481 ccatttttat ctatggattg ggccttttca tgtaatttat ctatggcctc agttatctcc
     3541 tccacctttt ccttcagtaa atcttcccta tcaaacattt tacctaaaat ttccatatta
     3601 cttttaaatg aatttaagta atctccacca tcaatattta aataaactgt aggtgctatt
     3661 tttctaagtt catcatagat atcaccttgc cttcctgaaa taataattag acctgggttt
     3721 atctcatata tggtttcaaa atttggctcc tttaaagaac ctatatcaac atattcatct
     3781 gctttatatt tatctaaata tgcaggtaga ctctgtttgg ccacccctat tacatctata
     3841 cctatattat ctaaagcatc taaaattcca taatcaaaga ctataattct ttctggattc
     3901 ttttttactt tttcttcacc taattcatgt ttgatggtga tcatctcttc tggattttct
     3961 ttgcctgtat cactgggctg aaaatcacat gatacaaaaa ttaacgacaa tataatcaac
     4021 ccaacaaata atatgctttt tcttatattt ttcacttcta ttctccctct ttcttaattt
     4081 taatagtata cacatagcct catattttct atatccttta ctgtaaagtc catatcaaat
     4141 attcttccta aagaattttt attaataatt ttatcagtat tgcctacctc cgctatcttt
     4201 ccatccttta aggccacaat atagtcagag taacaggagg caaagtttat atcatggata
     4261 actatggcaa tggtctttcc tagatcatct actagagacc ttaaaatctt cattatttcc
     4321 accgaatgtt tcatatctaa at
//
LOCUS       DATGSI010000219         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_489520, whole genome shotgun sequence.
ACCESSION   DATGSI010000219 DATGSI010000000
VERSION     DATGSI010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489520"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VK031_03375"
     CDS             <1..687
                     /locus_tag="VK031_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLS52987.1"
                     /translation="SYGYDPIEKKPLYRFRPGSMIFSIGSYGCNLACKFCQNWRIAHK
                     DPRTVKISDEDMINLAKAKGSIGIAYTYNEPTIWYEYVLHLSKLIKDEGLVNVLVTNG
                     FINKKPFEELLPYIDAINIDLKSMSNDFYKDICKGRSSPVLKTIERAADATHVEVTSL
                     LIDGLNTEPNKIEALATTIAEIDEEIPLHLSRYFPAYKMTRPATRVSTVIKAKEIAEK
                     YLRHVYLGNI"
     gene            complement(726..2162)
                     /locus_tag="VK031_03380"
     CDS             complement(726..2162)
                     /locus_tag="VK031_03380"
                     /inference="COORDINATES: protein motif:HMM:NF018240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrpR N-terminal domain-containing protein"
                     /protein_id="HLS52988.1"
                     /translation="MLNDNSEIYTTYSHEGVDTTLELLKIKDVDLILADTVNYVEARK
                     RGLRAILITSGEESVNIAFNEAISIGKVYRELQYENSLQKALQEMEDHIYCILNSNKD
                     LIYKSIPHIDEKVFQTLKEYTDKVKLSQEKKFFFYIHPSHYHCKIKCAYLSGEVLYLC
                     QIKKSEFSSKIYDVEMVDRRQALEDYLDSSFNITSSISGMEKQIQLASSSDKVVLLVG
                     EIGTGKTEVAKRIYSISEYNNNPLYIIDLTNMDTKQQRDLFHNPESPIFEVQSSFFLK
                     IDRNTEPAVVNDLIALIKNSRFSQNNKCIISYKLDFRKEDSDMLNLLMNSINTLPIYF
                     SPLREYRSDIRSLASIYLNEKNSSLGKHILGYDEKALNLLEQYSWPQNFIQFWRVLDQ
                     IIEICDQDYISVSLTREIIETEDNIFYYYEKNNAHGVNLDLNRPLEEIIRDIILIALE
                     NNNNNQTKTAEQLGVSRSTIWRYLKRRF"
BASE COUNT          703 a          364 c          360 g          776 t
ORIGIN      
        1 tcctatggct atgatccaat agagaaaaag cccctatata ggttcaggcc aggtagtatg
       61 atcttttcca ttggctccta cggttgcaat ttggcctgta aattttgtca aaattggaga
      121 atcgcccata aggatccaag gactgtaaaa atatctgatg aagatatgat aaatttagcc
      181 aaggccaagg gttctatagg aattgcctac acctacaatg agcctacaat atggtatgaa
      241 tatgttttac acctgtccaa actaataaag gatgaaggtc tagtaaatgt attggttacc
      301 aatggcttta ttaataaaaa accttttgaa gaacttttgc cctatattga tgccattaat
      361 atagatttaa aatccatgtc aaacgatttc tataaagata tttgtaaagg caggtcatcc
      421 cctgtactta aaactataga aagggcagca gatgccaccc atgtggaggt aactagcctg
      481 ctaatagacg ggcttaatac agagcctaat aagatagagg ctttggccac aaccattgct
      541 gaaatagatg aggagattcc acttcatcta tctagatact ttcctgccta taagatgaca
      601 agacctgcca ccagggtttc tacagttatt aaggccaaag aaatagcaga aaagtattta
      661 agacatgttt atctaggaaa tatttaataa taggcttata gattgttatt gatttttata
      721 ctaaattaaa atcttcgctt taaatacctc cagattgtgg atctggatac acctagttgt
      781 tcagcagttt tagtctggtt attattatta ttttccaagg ctattaggat gatatctctg
      841 atgatttctt ccagtggacg gttaaggtct agattaactc catgggcatt gtttttctca
      901 taataataga aaatattatc ttctgtttca ataatttctc tagttaagct tacagaaata
      961 taatcctggt cacagatttc tattatctga tctaagactc gccagaattg aataaaattt
     1021 tgtggccagg aatattgttc tagcagattt aatgcttttt catcatatcc caaaatatgt
     1081 ttacctaaag aactattttt ttcatttaaa taaatactag ctaaggatct aatgtcactt
     1141 ctatattccc ttaagggtga aaaatatata ggaagtgtat taatagaatt cattaataaa
     1201 tttaacatat ctgaatcttc tttccgaaaa tcaagtttat agcttataat acacttatta
     1261 ttctgggaaa atctactatt ttttattaag gctatcagat cattgacaac agctggctca
     1321 gtatttctat caatttttag aaaaaaactt gattggactt caaaaatagg agattcagga
     1381 ttgtgaaata aatccctttg ttgttttgtg tccatatttg ttaaatctat gatatacaaa
     1441 ggattgttgt tatattcgga gattgagtat atacgtttag caacttcagt ttttccagta
     1501 cctatttctc caactagaag aaccacttta tcactagagc tggccagttg gatttgtttt
     1561 tccatgccac taatggagct agttatgtta aaggaggagt ctaaataatc ctccaaggcc
     1621 tgtcttctat ctaccatttc tacatcgtaa attttactgg agaattctga ttttttaatt
     1681 tggcataaat ataaaacttc cccagacaga taggcacatt ttattttaca atggtagtgg
     1741 cttggatgaa tataaaagaa aaactttttc tcttgggaga gcttaacctt atctgtgtat
     1801 tctttcaatg tttgaaatac cttttcatca atatggggta tggatttgta tattagatcc
     1861 ttattcgaat tcaaaatgca atagatgtga tcttccattt cctgtaatgc tttttgtaaa
     1921 gaattttcat attggagctc tctatatacc ttaccaatgc ttatggcttc attaaaagca
     1981 atattaacac tttcctctcc agatgtaatt aatatggctc tcaaaccacg ttttcttgcc
     2041 tcaacatagt ttactgtatc ggccaagatt aggtctacat cctttatttt taatagttct
     2101 aaagttgtat ctacaccttc atgactataa gtggtgtaga tttcactatt atcattaagt
     2161 atagaacata attacttggc agtattggta agattaggat acc
//
LOCUS       DATGSI010000220         3656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_16308, whole genome shotgun sequence.
ACCESSION   DATGSI010000220 DATGSI010000000
VERSION     DATGSI010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16308"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2113)
                     /gene="lon"
                     /locus_tag="VK031_03385"
     CDS             complement(<1..2113)
                     /gene="lon"
                     /locus_tag="VK031_03385"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HLS52989.1"
                     /translation="MTHQYEVKKTKLPLIPLRGISIFPHMVIHFDVGRERSINALEQA
                     MLDDSYILLCTQKDPKIDDPDIDEFYHVGTVSKVKQMLKLPGGSIRVLVEGINRGKVT
                     DIVKKQPYYEVEVDELVYDPDEIEVDKIMEAGMRLVITDLEEYINLNSKVSSEVLMAV
                     SDIDDPGRLVDVIASYVFLKLEDDQKILETFDFYERLEVLHGILQKEIELLKIEDKIN
                     KRVKKQINKVQKEYYLKEQIKAIQKELGEDGDITGEAEEYKRKIQQIEMPKEAKEKVL
                     KEADRLTKMSPHSPETGVIRTYLDWIIDLPWNKESETSIDIKKSRKILDEDHYGLEDV
                     KERILEFIAIRKLTNSMKGPILCLVGPPGVGKTSIARSIARSLDREFVRMSLGGVRDE
                     AEIRGHRRTYVGSMPGRIIGLIRKAGTKNPVFLFDEIDKLSSDYRGDPASGLLEVLDP
                     EQNSTFTDHFLDIPFDLSKVLFITTANTTATIPAPLLDRMEVINISSYTGEEKLQIAE
                     KYLIPKQIKEHGLKEGNLQISEPAIRSVIDNYTREAGVRNLERNIANICRKAAKRIVE
                     ENLDTVRVNVGNISNYLGIPKYRYDLVEDKNQIGVANGLAWTQVGGETLSIGVNIMKG
                     KGKIQLTGKLGEVMKESAMAGISYIRANSDLLDIEGDFYKEMDIHIHVPEGAIPKDGP
                     SAGITITTAVVSALSNRPVYSN"
     gene            complement(2170..3450)
                     /gene="clpX"
                     /locus_tag="VK031_03390"
     CDS             complement(2170..3450)
                     /gene="clpX"
                     /locus_tag="VK031_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HLS52990.1"
                     /translation="MAKYEDKQLRCSFCGKPQDQVRRLIAGPNVYICNECIELCQEII
                     EEEFIDSYDYGMDEVPIPAEIKDTLDEYVIQQERAKRTLAVAVYNHYKRINKRVSKNK
                     DVELQKSNIVMIGPTGSGKTLLAQTLARILDVPFAIADATSLTEAGYVGEDVENIILK
                     LIQAADYDIERAEKGIIYIDEIDKIARKTENPSITRDVSGEGVQQALLKILEGTIANV
                     PPQGGRKHPHQEFIQVDTSNILFIVAGAFDGLDKIIQNRIGKNTIGFGAEIQTQEEKK
                     IGEILKNIQPEDLLKFGLIPEFVGRLPVVVTLEELDENALVKILTEPKNALVKQYKEL
                     FAMENVDLEFEEEALRLIAQKAIKRKTGARGLRSIIEETMLDIMYEIPSRTDVEKCLI
                     TKETIEQKGKPTLVLSEKTKRGRTEQSDKKESAS"
     gene            complement(3469..>3656)
                     /locus_tag="VK031_03395"
     CDS             complement(3469..>3656)
                     /locus_tag="VK031_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HLS52991.1"
                     /translation="EDIRIQAEKILEIRERINKILSERTGQPLEKIAKDTDRDYHLTP
                     EEAVEYGIIDEVIISGK"
BASE COUNT          907 a          802 c          543 g         1404 t
ORIGIN      
        1 cattgctata aacaggccta ttggataagg ctgagactac tgcagtggtt atggtaatcc
       61 ccgctgatgg cccatctttc ggtatggccc cttctggtac atgtatatga atgtccatct
      121 ctttatagaa gtctccctct atgtctaata gatcagagtt ggccctaatg taggaaattc
      181 ctgccatagc cgattccttc ataacttctc ctaactttcc tgtaagctgg attttgcctt
      241 tccccttcat aatattaacc ccaatggata gggtctctcc tcctacctgt gtccaagcca
      301 agccattggc aacacctatt tggtttttat cctcaaccaa atcataccta tatttgggaa
      361 tacctaaata attggaaata ttgcctacat ttaccctgac cgtatctaaa ttttcttcaa
      421 ctattctctt ggctgccttc ctgcagatat tggctatgtt tctttctaag ttccttactc
      481 ctgcttccct ggtataatta tctattacac ttcttatggc aggttctgaa atctgaagat
      541 ttccttcctt taatccatgt tcttttatct gctttggaat taaatatttt tcggctattt
      601 gtaacttttc ctcgcctgta tagctactaa tattaataac ttccatcctg tctaaaagtg
      661 gagcaggtat ggttgctgta gtgtttgctg tagttataaa aagcactttt gataggtcaa
      721 agggaatatc taggaagtga tcggtaaagg ttgagttttg ttctggatcc aacacttcca
      781 ataagccact ggctgggtct cccctatagt cactacttag tttatctatc tcatcaaata
      841 aaaatactgg atttttagtt ccggcctttc tgattaatcc tatgattcta cctggcatac
      901 tgcctacata ggtccttctg tgtcccctaa tttctgcctc atccctaact ccaccaaggg
      961 acattcttac aaattctcta tctaatgacc tggctataga tctggctata gaggtttttc
     1021 ccactcctgg tggacctact aggcaaagga ttggtccttt cattgaattg gtaagttttc
     1081 ttatggcaat aaactctaga atcctttctt ttacatcttc cagaccataa tggtcctcgt
     1141 ctaggatctt tctagatttt ttaatgtcta ttgaagtttc agactcctta ttccacggta
     1201 gatcaattat ccagtctaaa tatgttctaa taactccagt ttctggagaa tggggagaca
     1261 ttttagttag cctatctgct tctttcagca ccttttcctt agcttccttg ggcatttcta
     1321 tttgctggat ttttctttta tattcttctg cctctccagt aatatctccg tcctcaccta
     1381 attctttttg gatggccttt atctgttctt ttagataata ttctttttgg accttattga
     1441 tttgcttttt taccctttta tttatcttat cttctatttt taatagctct atttcttttt
     1501 gcagtatacc atgaaggacc tctaaccttt cataaaagtc aaaagtctcc aggatctttt
     1561 ggtcatcctc aagttttaag aatacataag aagctataac atccaccagc ctacctggat
     1621 catctatatc cgatactgcc attagaacct cagaagatac cttgctgttt aggttaatat
     1681 attcctccag gtctgttatt accaacctca ttccagcttc catgatttta tccacttcta
     1741 tttcatctgg atcatatacc agttcatcta cttctacttc atagtaaggc tgttttttga
     1801 ctatatcagt taccttgccc ctgtttatac cttccaccag gaccctgata ctgcctcccg
     1861 gtaacttcaa catttgcttt accttggaga ctgtacctac atgataaaat tcatctatat
     1921 ctggatcatc aattttagga tctttttgag tgcataataa tatataagag tcatctagca
     1981 tggcctgttc tagggcattt atagatcttt ctctgcctac atcaaagtgt attaccatat
     2041 gggggaaaat agatatgcca cgtaatggta ttagtggtag tttagttttc tttacttcgt
     2101 attgatgtgt caccttttat ccctccctag attaatgaaa gaaaaagacc cacagaaatt
     2161 tgtgagcctt taagatgcag attctttctt gtctgattgc tctgttctac ctctcttggt
     2221 cttctctgat agcactaagg taggtttgcc tttttgttca atagtttctt tagtaatcaa
     2281 acacttctcc acatcagttc tggatggtat ttcatacatg atatctaaca ttgtttcttc
     2341 tataatactt cttaggcctc tagcccctgt ttttctttta attgcctttt gggctattaa
     2401 ccttagagct tcttcctcaa attctaaatc tacattttcc attgcaaaca gttctttata
     2461 ctgttttacc agggcatttt ttggttctgt taagatttta actaatgcat tctcatctag
     2521 ctcttctaag gttactacca caggtaatct acctacaaat tcagggatca aaccaaattt
     2581 caataggtct tcaggttgga tattttttaa gatttctcct atttttttct cctcctgagt
     2641 ttggatttct gccccaaatc ctatggtatt tttccctatc ctattttgaa taatcttatc
     2701 taaaccatca aaggcacctg caactataaa aaggatattt gatgtgtcta cctggataaa
     2761 ttcttgatga ggatgtttcc gtccaccttg aggtggtaca ttggctattg tgccttccaa
     2821 aatctttagt aaagcttgct gaactccttc tccactaaca tccctagtaa tagatgggtt
     2881 ttctgtcttt cttgcaattt tatctatttc atctatataa attataccct tttctgctct
     2941 ctctatatca taatctgcag cttgaatcag ttttagaatt atattttcaa catcttcgcc
     3001 tacgtaacct gcttctgtta gagaggtagc atctgcaatg gcaaagggta catcaagtat
     3061 tcttgccaaa gtttgagcta gtaatgtttt tccagaacct gtaggtccaa tcattactat
     3121 attacttttt tgtagctcca catctttatt tttagaaacc cttttattaa ttcgtttata
     3181 atggttataa actgccacag ctagggttct ctttgctctt tcttgctgga taacatactc
     3241 atctaaggta tccttgattt ctgctggtat aggaacttca tccataccat aatcatagct
     3301 atctataaat tcttcttcta tgatttcttg acacagttct atacattcat tacatatata
     3361 tacatttggc cctgcaataa gcctacgaac ctgatcttga ggcttcccac aaaaagagca
     3421 tcgtagctgt ttatcttcat atttggccat aattacacct cttctgattt attttcctga
     3481 aataataacc tcatctatta taccatattc tacggcttct tctggtgtta aatgataatc
     3541 tctatctgtg tcttttgcaa tcttttctag aggttgacct gttctttcgc ttaggatctt
     3601 attgatcctt tctcttattt caagaatttt ctctgcctga atcctaatgt cttcag
//
LOCUS       DATGSI010000221         1002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_584619, whole genome shotgun sequence.
ACCESSION   DATGSI010000221 DATGSI010000000
VERSION     DATGSI010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584619"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VK031_03400"
     CDS             complement(<1..404)
                     /locus_tag="VK031_03400"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLS52992.1"
                     /translation="MKICIDAGHGINTAGKRTPPFKDGSFMKEFEFNVKVAEYLAEEL
                     RKYKNVEIMFTHDHPSGKRDVPLKERTDKANRWKADLFVSLHANAYGDGKSFNSAKGT
                     STFIHHIVPQRTIDIAQAIHRQLIKDLGRRDRG"
     gene            complement(405..659)
                     /locus_tag="VK031_03405"
     CDS             complement(405..659)
                     /locus_tag="VK031_03405"
                     /inference="COORDINATES: protein motif:HMM:NF027405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HLS52993.1"
                     /translation="MDIGGYLIEEALIMIPVLYILGNFLKSLEGFKDKYIPIILLVVS
                     IFFTPLVIGKFNADNVVQGILVAGTTVFANQVYKQSRRDE"
BASE COUNT          289 a          195 c          159 g          359 t
ORIGIN      
        1 cctctatccc ttctaccaag atctttaatt agctgcctat gaatggcctg ggctatatct
       61 atggttcttt gaggaactat atggtggatg aaggtgcttg tacccttggc agagttgaag
      121 cttttgccat ctccataggc attggcatgg agggatacaa ataagtctgc cttccaccta
      181 ttggccttat cggtcctttc ttttaggggc acatctcttt tgcctgaagg atgatcatgg
      241 gtaaacatga tttccacatt tttatatttt ctcagctctt ctgccaagta ttccgctacc
      301 ttcacattaa attcaaattc cttcataaaa ctaccatcct tgaaaggtgg agtccttttt
      361 ccagctgtgt ttattccatg gcctgcatca atacatattt tcatttattc atccctccta
      421 gactgcttgt atacctgatt ggcaaatact gtagtaccag ctaccaatat gccttgtacc
      481 acattgtctg cgttaaattt acctattact agaggggtga aaaatatact gactaccaat
      541 aggattattg gtatatattt atccttaaat ccttccaaac tttttaaaaa gtttcctaat
      601 atatataaga caggaatcat aatcaaagct tcttctatta aatagcctcc tatatccata
      661 aaatacctcc taatcttctt tattaccata atatgacttg tctatatttt tggttcttaa
      721 aataaaacca cctccaaggt ataagttctt tgaaggtggt tttattacta tttttaaaat
      781 tataggagct atctttatca taaggtggcc gtacgtgtca acattacttt ttgtacctat
      841 gtataggata taagtggatt ttaaagacta tctaagcatt aagttatttg ataactacct
      901 gtttagtttt aacctttaca gagttagaca aaaaatgaca tacatcagca gctatgctaa
      961 ttaatgcggg attgaatata ggggcattat ctgcaagatt ag
//
LOCUS       DATGSI010000222         1175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_16361, whole genome shotgun sequence.
ACCESSION   DATGSI010000222 DATGSI010000000
VERSION     DATGSI010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16361"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1175)
                     /locus_tag="VK031_03410"
     CDS             complement(<1..>1175)
                     /locus_tag="VK031_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52994.1"
                     /translation="NDKDYGSGYLVKKSSFHNELNIDPDIYPDVGIINPIQVSNPKQE
                     FFFSQFSYIFYPFYSNEKISFENYKQGIGVPNSAIQRLKDNSDIYKITTLQNLYNTAG
                     NETYVEFSKAVTYNSYNDNLDNLFIGGSKVNYEEIPYVALLDMYGAAVPGEGNPMSPF
                     GWLWDISYNTVSNMIYGIGLYEGKLVDQRKNVNNKLLELQELYKEFNASLIKKGLCSP
                     SPVMYGEFGYYESKERYPCNKEIWGDLAGKPIRHHRFPKRDIHDNMGNIYTLGIRFDL
                     DEIKDIIKNSTKLTQEEKDDIVGFKILRGNRRNNKSVIAKGLINNVGVYSDEKGRTRY
                     YPNYPYNDLRADPFLSIGQTEDDSGKNIGLQLNGFTNPDEYQRRFTFHSPDTSFYQP"
BASE COUNT          373 a          207 c          142 g          453 t
ORIGIN      
        1 agggctgata aaatgatgta tcaggtgagt ggaacgtaaa ccttctttgg tactcatctg
       61 ggttagtaaa tccatttaat tgtaaaccga tattctttcc actatcatcc tctgtttgtc
      121 ctatagataa aaaaggatct gcccttaaat cattatatgg gtaattagga taataccttg
      181 tccttccttt ttcatcacta taaactccta cattattaat taaacctttg gcgataaccg
      241 atttattatt tcttctattc cctcttaata ttttaaaccc tacaatatca tctttttcct
      301 cctgtgttaa ttttgtggaa ttttttatta tatcttttat ctcatctaaa tcaaacctta
      361 ttcctaaagt gtatatatta cccatattat catggatatc tcttttaggg aatctatgat
      421 gtctaatagg tttacctgct aaatctcccc aaatctcttt attacaagga tatctttctt
      481 tactttcata atacccaaac tctccataca taacaggaga aggagaacat agtcctttct
      541 ttattagaga ggcattaaac tccttatata attcttgtaa ttctaataat ttgttattaa
      601 cattttttct ttgatctacc aattttcctt catataatcc aataccataa atcatatttg
      661 atactgtatt atatgatata tcccatagcc acccaaatgg tgacatagga tttccttcac
      721 caggaactgc tgcaccatac atatctaata atgcaacgta aggaatttct tcatagttta
      781 ctttacttcc acctataaaa agattatcaa gattatcatt gtatgaatta taagttaccg
      841 cttttgaaaa ttctacataa gtttcatttc ctgcggtatt atacaaattt tgcaatgtag
      901 tgattttata tatatctgag ttatccttta acctttgtat tgcagaatta ggaactccta
      961 tgccctgttt atagttttca aagcttattt tctcattaga ataaaaggga taaaaaatat
     1021 aagaaaattg tgaaaaaaag aactcttgct taggattaga tacttggata ggattgataa
     1081 tacctacatc aggatatata tctggatcta tatttagttc attatgaaaa ctggatttct
     1141 taactaagta tcctgatcca taatctttat cattg
//
LOCUS       DATGSI010000223         1663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_346136, whole genome shotgun sequence.
ACCESSION   DATGSI010000223 DATGSI010000000
VERSION     DATGSI010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346136"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..479
                     /locus_tag="VK031_03415"
     CDS             144..479
                     /locus_tag="VK031_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HLS52995.1"
                     /translation="MANRKRNVQLHFMVTEHERSLIDEKMAQLGTKNMGAYLRKMAID
                     GYIIHLDLSDIRELVTLLRRTSNSLNQLTKRVHQTGNIYGEDIEALRESYDRLWDMAD
                     EILLRLSTI"
     gene            1128..>1663
                     /gene="rhuM"
                     /locus_tag="VK031_03420"
     CDS             1128..>1663
                     /gene="rhuM"
                     /locus_tag="VK031_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HLS52996.1"
                     /translation="MQIRNSTVDFMVFTKDAGSDSIEVRVQDGDVWLTQKAISMLFDV
                     DRTVVTKHLKNIFETGELEEDSVCAKFAHTADDGKTYQYKFYSLSAIIAVGYRTNSER
                     ALQFRQWATKVLDTFTKQGYVLDKNRLINGQIFDEDYFEHLISEIQEIRASERRFYQK
                     ITDIYATAVDYSKDSKVT"
BASE COUNT          578 a          245 c          330 g          510 t
ORIGIN      
        1 atagaagtat caggctcgca agaacaggca caaaaggaga aatctaaacc tgaacgtccg
       61 tctgtattaa atagcttaaa acaatcccca caattaaaca gagaatttaa agttaaacaa
      121 gataaatgtg cggaggtgag gtgatggcaa atcgtaaaag aaatgtccaa ctacatttta
      181 tggtaacaga gcatgaacgc agtttaatag atgaaaagat ggctcaactt ggtacaaaga
      241 acatgggagc ctatctccgt aaaatggcta tagacggcta tattatccac ctagacctat
      301 cagatatacg agagttagta acactccttc gtcgtactag caacagctta aatcagctta
      361 caaagcgtgt ccatcagaca ggaaatatat atggtgagga tatagaggct cttagagaaa
      421 gttatgacag gctttgggat atggcagatg aaatactgtt gagactttct accatttaaa
      481 attcataaga tatacaatat cctttttatt tggtatgttt tttatataag atgtattaag
      541 ggaaaagggg gatttcatta tgcgaatact acttaaaata ttattatttc caattactct
      601 aatattgacc attattctgc tcatctgtga gtttatatgt atgtttggaa caatgctact
      661 ttccatccta gccttgctcc tgttcgtcct agcactagct actatgattt tcttgaaaga
      721 tgtgcaggat ggattaaaag caatggtact tgcctatctt attagtccct atggaatacc
      781 cttatttgct acttggttgt taggaaagat tggggatata aatcaaagac tgaaatctat
      841 ttaattatta aagagggcga aagcattttt atgctttcac ccttaattga cacacagcag
      901 gagtgccagc ggaaagcaag ggcggcgtag ccgttcatct cgacccttgc tttttgatgg
      961 tacacctgct attttttctg tgtttgatat ttgatgggca aaggattata atttaatata
     1021 ataggtaaat aggatattaa ataatagatg atataaaaat tcattataca tacgattggt
     1081 ataaaatatc gaaattagaa aggaatataa atatggaagg taagaatatg caaatacgca
     1141 atagtacagt agactttatg gtctttacta aagatgcagg tagtgattct atagaagtcc
     1201 gtgtacaaga tggtgatgtg tggttaaccc aaaaagccat atctatgcta tttgatgtgg
     1261 atagaactgt ggtgacaaag catttgaaaa atatatttga aacaggtgaa ctagaagaag
     1321 actcagtatg tgcaaaattt gcacatactg cagatgatgg taaaacctat caatataaat
     1381 tctattcctt gtcagccata attgccgttg gttaccgtac aaattctgaa agagcattac
     1441 agtttaggca gtgggcaact aaagtcttag atacttttac aaagcaggga tatgttttag
     1501 ataaaaatag attaattaat ggacaaattt ttgatgaaga ttattttgaa catttgattt
     1561 ctgaaattca ggaaataagg gctagtgaaa gacggtttta tcagaagatc actgatatat
     1621 atgctacagc ggttgattat tctaaagaca gtaaagttac cag
//
LOCUS       DATGSI010000224         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_248890, whole genome shotgun sequence.
ACCESSION   DATGSI010000224 DATGSI010000000
VERSION     DATGSI010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248890"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VK031_03425"
     CDS             <1..1278
                     /locus_tag="VK031_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52997.1"
                     /translation="PELESIGNGEKILHEGKVRTIESDTLFIDVTEEGGLKVEAEQEG
                     IERYIVNNLYQGSEERGTLSKPFKTIQTALNKLIGNGTKSEPENIGAVIVVQQGNTYN
                     FTGTLDIRGLKMVIEENTRINSNPNSGWFIDTTAINESKKTDIEVTIRSGARIDLKKN
                     GIRTRGVATGSDGYKRVTLKGEGVIVNATNNTQYKVIDCNSEDNSNYETGIEGGDIHI
                     QGPTIWSHRGKTIEIGGSKNTIYITNSVVRAGTSDATTASVTPFIIRKGSLYISNSDI
                     YFASQDTRNIGIEMKNKEGRLQIINSVIQPQNKVKKLVYDNSLETSTPRIIIGYTYTR
                     YGEIEDIIDINTPKIKNDLRLDVYYNNFHTGRIREEVDLTNWNKKSTTNIIKGIVIDN
                     LIRFQDKSTAQSSGYPKGTKFINNQTNTVDMIT"
     gene            1382..2056
                     /locus_tag="VK031_03430"
     CDS             1382..2056
                     /locus_tag="VK031_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS52998.1"
                     /translation="MKTIRDLTDSIITATRQNYRDRKMSRRYIISVYKRIMKDLIAQK
                     IRDRSLYREENLYVDIDCYEFEEIENKKCDIIEFRMCESIMKGRKRLPELIYTRYGDS
                     IRMVTNLDGSVKINKTTPSKYIRDRTRRSYRAEPQYYVRDGYPYLLNTKMEAGTISVL
                     TLEIEEAEKARCGDIDECKSSLDYPLIGSDKLINTAYQQTLQEILRAYIQVRPDENPD
                     NNENNI"
BASE COUNT          956 a          296 c          429 g          542 t
ORIGIN      
        1 ccagaactag aaagtatagg gaatggagaa aagatacttc acgaaggtaa ggtaaggact
       61 atagaatcag acacgttatt catagacgtt actgaagaag gtgggcttaa ggtagaggca
      121 gagcaagaag gtatagaaag gtatatagtc aataacctgt accaaggatc tgaagaaaga
      181 gggaccttaa gtaaaccttt taagactatt cagacagctt taaataaatt gatagggaac
      241 ggaactaaat ctgaacctga aaacatagga gctgttattg tagtacagca aggaaacacc
      301 tataatttta caggtacttt agacataaga gggttaaaaa tggtaataga ggaaaacaca
      361 agaatcaatt ctaaccctaa ttctggatgg tttatagaca ctactgctat aaacgaatcc
      421 aagaagacag acatagaggt gacaataaga tctggagcta gaatagattt aaaaaagaat
      481 ggaattagga ctagaggagt ggctactgga agtgatggat ataaaagggt tacattaaaa
      541 ggggaaggag taatagtaaa tgccactaac aatactcaat ataaagtcat agattgtaat
      601 tctgaagata acagtaatta tgaaacagga attgaaggag gggatataca tatacaaggg
      661 cctactatat ggagccatag aggtaagaca atagagatag gagggtccaa aaatacaatc
      721 tacataacaa atagtgtagt aagagctgga acatctgatg caaccactgc ttcagtaact
      781 cctttcatta tcagaaaagg cagtctatat attagtaaca gtgacattta tttcgcctca
      841 caagatacta gaaatatagg aatagaaatg aaaaacaaag agggcaggtt acaaataata
      901 aactctgtta tacaacctca aaacaaggtc aagaaactgg tatacgacaa ctcattagaa
      961 actagtacac ctagaataat aataggatat acttatacta gatatggaga aatagaagac
     1021 ataatagata taaatactcc taagataaaa aatgatctta gactagatgt atactataac
     1081 aacttccata caggaagaat aagagaagaa gtagatttaa ctaattggaa taaaaagtca
     1141 actacaaaca taattaaggg tattgtaata gacaatctta taagattcca agataaaagt
     1201 acagcacaat cttcagggta ccctaaaggc actaaattca taaacaatca aacaaacact
     1261 gtagacatga taacataaaa ggttttattt tggttttcct tttatgttat agggagggat
     1321 tgaaccctcc cttattttta acaatttatg taaataactt aaaaagtagt atatttgaac
     1381 aatgaaaact ataagagatt taacagatag tatcataaca gctactagac aaaactacag
     1441 ggataggaaa atgtctagaa gatatataat atctgtatac aaaaggatta tgaaagatct
     1501 tattgcacaa aagataagag ataggtcttt atatagagaa gaaaatcttt atgtagatat
     1561 tgattgttat gagtttgaag aaatagagaa caaaaaatgt gatattatag aattcagaat
     1621 gtgtgaaagt ataatgaaag gaagaaaaag attgcctgaa cttatttaca ctagatatgg
     1681 agattctata agaatggtaa ctaatttaga cggaagtgtt aaaataaata agactacccc
     1741 atctaagtat ataagagaca gaactagaag aagttatagg gcagagccac agtattatgt
     1801 aagagatgga tacccttatt tattgaacac taagatggag gcaggaacca taagcgtgtt
     1861 aactttagaa atagaagaag cagaaaaggc caggtgtgga gacatagacg aatgtaagag
     1921 ttcgttagat tatcctttaa tagggagtga taaattaata aacacagctt atcaacaaac
     1981 tctacaagag attttaagag cttacataca agttaggcca gacgagaatc cagacaacaa
     2041 tgagaataat atatgaaaga atacaaatac aagcataacc caaacttcac taaaacaaag
     2101 aaaaaaagga cagtagaact agagaaaata ccctaccata aaagaaagcc caaaagctgg
     2161 acactaaaca tgttgaaacc aggcgaccaa aaagggtatt ggttttacag gaacaattca
     2221 aaa
//
LOCUS       DATGSI010000225         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_297874, whole genome shotgun sequence.
ACCESSION   DATGSI010000225 DATGSI010000000
VERSION     DATGSI010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297874"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VK031_03435"
     CDS             complement(<1..1314)
                     /locus_tag="VK031_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HLS52999.1"
                     /translation="MLKIEGIIEEIIFRNESNGYTVAKLNTSDGVITIVGNAAIINLE
                     EMVEVEGDWVYHNTFGEQFNFTSIKTTVPSTLKGIENYLSSGLLPYVGPKTAKNIVDR
                     FGLDSLDIIQYNPERLKEIPGIGEKKLEKIMEAYEEQRELRDIMVYLQQYDITVNYGI
                     KIYKEYGRDTIRVISENPYRLSEDIYGIGFKTADKIARKMGIDPNSPYRIEAGLKYIM
                     MDSAGEGHCYLPKEELLDRAGQLLNVEEDLVEAAIRDLAVKNSFHIVTENNESVVFYA
                     PYHGAENNVARKIVELSRVEFDDLSIDVDKKILEIEKKEKIKFGNKQKLAIKEAINNG
                     VMIITGGPGTGKTTTINAIIQVYESLDLKVLLAAPTGRAAKRMTETTGRESKTIHRLL
                     EFSYLEDDMAFSRDEDSPIEADAIIIDEASMIDILLMNSLLKAIHP"
     gene            complement(1410..1553)
                     /locus_tag="VK031_03440"
     CDS             complement(1410..1553)
                     /locus_tag="VK031_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HLS53000.1"
                     /translation="MEELYSSIANLGFPIVISIYLLVRIEGKLDQLTASINELTKVIS
                     SIR"
     gene            complement(1612..1830)
                     /locus_tag="VK031_03445"
     CDS             complement(1612..1830)
                     /locus_tag="VK031_03445"
                     /inference="COORDINATES: protein motif:HMM:NF022597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HLS53001.1"
                     /translation="MERRKLELDFLDVEGRRFRLSIDDPREDLETAEIEAAMEDIIDY
                     NIFQSNYGDLISPNMARIVTTTVEEIKF"
     gene            complement(1860..2081)
                     /locus_tag="VK031_03450"
     CDS             complement(1860..2081)
                     /locus_tag="VK031_03450"
                     /inference="COORDINATES: protein motif:HMM:NF019487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HLS53002.1"
                     /translation="MAVVGEKEKVTLRLGLDAGLVDGQQKIQTKSFTRIKTTAENEDL
                     YNTALVLAGLQSKDLLEVKKVEESILTSQ"
     gene            complement(2219..>2975)
                     /gene="metK"
                     /locus_tag="VK031_03455"
     CDS             complement(2219..>2975)
                     /gene="metK"
                     /locus_tag="VK031_03455"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009344844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HLS53003.1"
                     /translation="FMPMSIFLAHKLAKRLAYVRKANILDYLRPDGKTQVTVEYQGGK
                     PVRIENVVVSTQHKPETELEQIRQDIIKHVILEIIPEDMIDENTKYYVNPTGRFVIGG
                     PMGDAGLTGRKIIVDTYGGFGRHGGGAFSGKDPTKVDRSASYAARYIAKNIVAAGLAD
                     RCEIGLSYAIGVAKPLSIYVDTFGTGRVSDGKIIELINENFDLRPAAIIRDLDLKRPI
                     YRQIAAYGHFGREDLDLPWERLDKVDNLKKILD"
BASE COUNT          794 a          680 c          418 g         1083 t
ORIGIN      
        1 tggatgaata gcttttaaga gactattcat aagcaggata tctatcatgg atgcctcatc
       61 aattataata gcatctgcct ctatgggact atcttcatcc ctgctaaagg ccatatcatc
      121 ttcaaggtag gaaaactcca ataacctatg aatagtttta ctttccctac ctgtagtttc
      181 tgtcatcctt ttggctgccc ttcccgtagg tgcagccaaa agcaccttta aatctaaact
      241 ttcatatacc tggataattg catttatggt ggtagtctta cctgttccag gtccacctgt
      301 aattatcata accccattat ttatagcttc tttaatggcc agcttttgct tatttccaaa
      361 ttttatcttt tctttttttt ctatctctaa aatcttctta tccacgtcta tagaaagatc
      421 atcaaattct accctggata attccactat ctttctggcc acattatttt cagccccatg
      481 gtagggggca taaaaaacta cactttcatt gttttcagta actatgtgaa agctattttt
      541 tactgccaaa tctcttatag cagcctccac caagtcttct tccacattta atagctgacc
      601 tgccctatct aacagctctt ctttaggcaa ataacaatga ccctctcctg cactatccat
      661 cataatatat tttaggcctg cctctattct atagggagag ttaggatcaa tgcccatttt
      721 cctggctatt ttgtctgccg ttttaaaccc gataccataa atatcctctg ataagcggta
      781 agggttttca cttattaccc taattgtatc cctgccatat tctttgtata ttttaattcc
      841 ataattaact gtaatatcat attgctgcaa ataaaccata atgtccctta attccctctg
      901 ttcttcatag gcctccatta ttttttctaa tttcttttct ccaattccag gtatttcctt
      961 taatctttct ggattatatt gaataatgtc aagagaatca agaccaaatc tatccactat
     1021 gttttttgct gttttaggtc ccacataggg taataggccg gaggataagt agttttctat
     1081 tccctttaag gtggaaggaa cagttgtctt tatactggtg aaattaaact gttctccaaa
     1141 ggtattatgg taaacccagt ctccttccac ctccaccatt tcctccaaat taattattgc
     1201 agcatttcct actatagtta ttactccgtc agaggtattt aattttgcca ctgtgtatcc
     1261 attgctttca tttctaaata tgatttcctc aataatccct tcaattttta acaaatcaat
     1321 acccccagtt ctatgtggat attataccat atactgaatt attttttcca tttatggccg
     1381 gtctcatcca atagcttaaa tctaaaagat tatcttatac tggatattac cttggtcaat
     1441 tcattgatgc tggctgttag ttgatctaac tttccttcta ttctaacaag caaatatatg
     1501 gaaatgacaa tagggaagcc taagttggct atactagaat acaactcttc cattgttctt
     1561 acctccttgt atctattata tattttaaaa gggccctggt gggccctttt cttaaaattt
     1621 tatttcttca actgtagttg ttactattct agccatattt ggactgatta ggtcaccata
     1681 attagattgg aaaatgttgt aatcaataat atcttccatg gcagcctcaa tttctgccgt
     1741 ttccagatcc tccctgggat catctatgct taatctaaat cgccttcctt ccacatctaa
     1801 aaagtctaat tctaatttcc ttctttccac cttaaaaccc cctttccttt aattggctac
     1861 tactgactgg ttaatatact ttcttcaacc tttttaacct ctaataggtc cttgctttgt
     1921 aggccagcca aaactaatgc cgtattgtac aggtcttcat tttcagcagt agttttaatc
     1981 ctggtaaagg atttagtttg tattttttgt tgcccatcaa caaggccagc atctaggcct
     2041 aaccttaaag ttaccttttc tttttcacct acaactgcca cattttcacc cccttctttt
     2101 aggctagttt aaaggcccta ccagaccctt caccttatat atggaaggag gtttaatatt
     2161 ttaccatcaa aaaagaccct tacatttcta taagggtctt ttaagcttct attttgtttt
     2221 agtcaagaat ctttttcaaa ttatcaacct tatcaagtct ttcccaaggt aaatctaaat
     2281 cttctctacc aaagtgacca taggctgcaa tctgtctgta tataggtctt tttaagtcta
     2341 agtcccttat aatggctgca ggtcttaggt caaagttttc attgattaat tcaatgattt
     2401 taccatctga aaccctacct gtaccaaagg tatccacata tatagacaaa ggcttcgcca
     2461 ctcctatggc gtaggatagg cctatttcac acctgtccgc caatccagca gctactatat
     2521 ttttcgcaat gtatctagct gcgtaggaag cagatctatc tacctttgtt gggtccttgc
     2581 ctgaaaaggc cccaccaccg tgcctgccaa agccaccata ggtgtctact attatctttc
     2641 taccagtcag gcctgcatcg cccataggcc cgcctattac aaatctgcct gttggattta
     2701 cataatactt tgtattttca tctatcatat cttcaggtat aatttccagg attacatgtt
     2761 taattatatc ctgcctaatt tgctctaatt ctgtctctgg cttatgttga gtagatacaa
     2821 ccacattttc aatcctaact ggtttgcccc cctgatactc tactgttact tgagtttttc
     2881 catctggtct taaataatct aagatattgg cctttctaac ataggccaat cttttagcca
     2941 gcttgtgggc caagaatata gacattggca taaat
//
LOCUS       DATGSI010000226         1774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_584949, whole genome shotgun sequence.
ACCESSION   DATGSI010000226 DATGSI010000000
VERSION     DATGSI010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584949"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1333)
                     /locus_tag="VK031_03460"
     CDS             complement(161..1333)
                     /locus_tag="VK031_03460"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HLS53004.1"
                     /translation="MRYNFDEIIDRRNSDSIKWGQLEELYGDEDVLPLWIADMDFRSA
                     DEIVEAIKERADHGVFGYIYMKDSFYQSIIDWVKRRFNWEIKKEWILFTPGVVMGFNI
                     GVRELVDKGDKVLVQPPVYPPFYRVLDNNGRVPLENPLKHDGERYVMDFEDLEEKAAD
                     SSLIMLCNPHNPVGRVWTREELDRLGQICVENDITIISDEIHCDFTLKGVEHTPMASL
                     SKELEQQTITLMAPSKTFNIAGLTTSVAIIPNEELREKYTKALEVMEVGNPTIFGALA
                     LETAYKHGEEWLDEVMVYVEDNIDYAIQYIKENIPEIKVDRPDGTYLLWLDFRSLNKS
                     NDEIEKALIEEGKVALNDGRPYGTGGDGFFRLNIGCPRSILKDGLQRIEKAVKGMK"
     gene            complement(1497..>1774)
                     /locus_tag="VK031_03465"
     CDS             complement(1497..>1774)
                     /locus_tag="VK031_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HLS53005.1"
                     /translation="LSMEPDVMLFDEPTSALDPEMVGEVLKVMKDLASTGLTMLVVTH
                     EMEFAKDVSDRVVFMDNGVIAEEGSPEEIFNNPKKARTREFLGRLLN"
BASE COUNT          534 a          398 c          222 g          620 t
ORIGIN      
        1 aaaacaaata cttttttcaa tcttaacaac tcctaatttt actttgtatt cttatattct
       61 aacacataaa gtagaaaaat tatattatag cccctaattt ctatctatct aatcttataa
      121 atttaaggct aagaaataac cttcttagcc ttaataatct ctacttcata ccctttactg
      181 ctttttcaat cctttgtagt ccatccttta atatagatct gggacaacct atatttaatc
      241 taaagaaacc atctcctcct gttccatagg gtcttccgtc attaagagca accttgcctt
      301 cttctattaa ggctttttca atttcatcat tagatttatt taaacttcta aagtctagcc
      361 ataataaata agttccatca ggcctgtcta cctttatttc aggaatattt tcctttatat
      421 attggatagc ataatcaata ttatcttcca catataccat tacttcatct agccattctt
      481 ctccatgctt gtaggctgtc tcaagggcta gggccccaaa gatagttggg ttcccaactt
      541 ccataacctc taaagccttt gtatactttt ctcttagctc ctcatttgga attatagcaa
      601 cagaagtcgt aaggcctgct atgttaaagg ttttactagg tgccataagg gttatagttt
      661 gctgttccaa ttctttagat agtgaagcca ttggagtatg ttccacacct tttagggtga
      721 agtcacagtg gatctcatca gatataatag taatatcgtt ttccacacaa atttggccta
      781 gcctgtccaa ttcttcccta gtccaaaccc tgcctactgg gttatgaggg ttgcataaca
      841 taattaggct agaatcagct gccttctctt ctagatcttc aaaatccatt acataccttt
      901 ccccatcatg cttcaaaggg ttttccagcg gcactctacc attattgtct aacaccctat
      961 aaaatggtgg gtatactgga ggctgaacta aaaccttatc tcccttatct accagttctc
     1021 taactcctat attaaatccc attaccactc ccggagtaaa aaggatccat tctttcttta
     1081 tttcccaatt aaaccttctt tttacccaat caataataga ttgatagaag gaatccttca
     1141 tatatatata accgaaaact ccatggtctg ccctctcttt tatggcttca acaatttcgt
     1201 ctgcagatct aaaatccata tcggcaatcc ataaaggtag aacatcttcg tctccgtaaa
     1261 gttcttccag ttgaccccat ttaatagaat cagaatttct tctatctatt atttcatcaa
     1321 aattatatct catattgctt caactccttt aatgtttatt atttgtacca aataacaatt
     1381 ctatcaaatt gaaaatattt tacaatgaga aagagacagt tattttatac cctttgtaaa
     1441 caaaaaatcc ctccatttta taaatagtta taaaatggaa ggaataagtt ttaatcttaa
     1501 tttaacaatc tgcccaaaaa ctctctagtt ctagcctttt taggattgtt aaatatttct
     1561 tcaggacttc cttcttcagc aattacacca ttatccataa atactaccct atctgataca
     1621 tctttagcaa attccatctc atgggttacc accagcatgg tcaaccctgt actggctaaa
     1681 tccttcataa ccttaagaac ttcaccaacc atttcaggat ccagggctga tgttggctca
     1741 tcaaatagca ttacatcagg ctccatggat aggg
//
LOCUS       DATGSI010000227         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_440549, whole genome shotgun sequence.
ACCESSION   DATGSI010000227 DATGSI010000000
VERSION     DATGSI010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440549"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="VK031_03470"
     CDS             complement(<1..1468)
                     /locus_tag="VK031_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53006.1"
                     /translation="MDNKNKKFNDTNTVTVSIKLADIPQPVQRGKGGRLGDEVYTVYG
                     NNNLYPNYLLSLYNNSSIHKSIIDSKVNYILGDGIIDPDSTEELDFDINDKENIEDLL
                     RKVTKDFLIFNYFAIEVVYAKDGSIYELNHLEGSKIRTNRTHTHFWYSEDWSNPRIET
                     LDFNKWSKKIDGDLESKVFFYSGYTPSVNSTYPPPEYSGAIKSIEIDKAIKDFHLNNI
                     SNSFSGNTIISFFRGEPTPEIKDEIVESITGSYSGTKGDKVIFQFLEKDEKEPEITQL
                     SAGDWNEAYLSLRKDTIEDIIISHSVTSPMLCGIKTEGQLGGATELETAYQIFNKLWV
                     SVTRREVIGALNSLLEEQYGVIDIKDNGKLFPVELSDTLREKVYQIDEIRALEGLPEL
                     PNGEGKRLISDPIVVKEEAVREAESMGFREDKNLKEGINPYKELSEEEELEFSSIGID
                     RKDAVFLESHKFSKEKEDKLNISFSDDSLSEHLIKNRLT"
     gene            complement(1479..1880)
                     /locus_tag="VK031_03475"
     CDS             complement(1479..1880)
                     /locus_tag="VK031_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53007.1"
                     /translation="MMIVLNKSKESQFIRIVWDFYPNSDDTQIMVLYDRYSKKEYTVD
                     LGFNNSNSTYRYGRYIINKEDIEELLEGDYLYRIYNQSNINMCEVLSPLSRGILRVEL
                     SLSDDKVVERQPIVLDSEESSGFIVFDDSNL"
BASE COUNT          670 a          428 c          252 g          775 t
ORIGIN      
        1 cagttaatct atttttaata agatgttctg aaagactgtc atcagaaaaa gaaatattta
       61 acttatcttc tttctctttg ctaaacttat gagattctaa aaataccgca tcttttctat
      121 caatacctat agagctaaat tctaactctt cttcttccga taattcctta taagggttga
      181 ttccttcttt aagattttta tcttctctga accccataga ttcagcttct cttacagctt
      241 cttctttgac tactataggg tctgaaatta atctcttacc ttctccatta ggaagttctg
      301 gaagtccttc taaagctctt atttcgtcta tctgataaac cttctctcta agagtatcac
      361 ttaactctac agggaatagt ttaccgttat ctttaatatc tataactcca tactgttctt
      421 ctaataggct gtttaaagcc cctataacct ctcttctggt tacgcttacc caaagcttat
      481 taaatatttg ataagctgtc tctaactctg tagcaccccc taactgacct tctgtcttaa
      541 taccacataa catagggctg gttacactat gagagattat aatatcttct atagtatctt
      601 tacgaagaga tagataagct tcattccaat ctcctgcact taattgagtg atttctggtt
      661 ctttctcatc tttctctaag aattggaaaa taactttatc tcccttagtt ccagagtaac
      721 ttccagtgat agattctaca atctcatctt taatctctgg tgtaggctct cccctaaaaa
      781 aggatataat ggtattacca ctaaaagagt tactaatgtt gtttaaatgg aaatctttaa
      841 tagctttatc aatctcaata gacttaatag ccccactata ctctggaggt gggtaagtac
      901 tattaacaga aggggtataa ccactataaa agaaaacctt agattctaag tctccatcta
      961 tcttcttact ccacttatta aagtctaaag tctctattct aggattactc caatcttcac
     1021 tataccaaaa atgagtatga gtcctattag tcctaatctt agatccttct aaatgattta
     1081 attcatatat tgaaccatcc ttagcataca caacttcaat agcaaaatag ttgaatatta
     1141 agaagtcttt agttactttc cttagtaagt cctctatatt ctccttatca tttatatcaa
     1201 agtccaactc ttcagtacta tctgggtcta taataccgtc tcctaaaata tagttaacct
     1261 tagaatctat aatagattta tgaatagaac tattgttgta taagcttagc aaataattag
     1321 ggtataagtt attatttcca tatactgtat aaacctcgtc tcctaatctt cctccctttc
     1381 ctctttgaac tggttgggga atatctgcta acttaataga taccgtaact gtattggtat
     1441 cattgaactt tttattttta ttatccattt taatttactt ataaattact gtcgtcaaac
     1501 actataaatc cagaggactc ttcactatct aatactattg gctgtctttc tactacctta
     1561 tcatcagaaa gacttaattc cactcttaaa atacccctag acaaaggaga taatacttca
     1621 cacatattaa tgttagactg attatatatc ctatacagat agtctccctc taacaactct
     1681 tcaatatctt ctttgtttat tatatatctt ccatacctgt aagtagagtt agagttatta
     1741 aatcctaaat ctactgtgta ctccttttta ctatatctgt cataaagaac cattatttga
     1801 gtatcatcag aattagggta gaaatcccac actattctta tgaactggct ctcttttgac
     1861 ttatttaaca ctatcatcat tgctaatttc ctcccgttaa tgcttactta gttaatgtat
     1921 atagaaataa aaaaccccct tcgataaaga agagggataa cctaaataat ccaaaagaaa
     1981 aatgaattaa gaaaacaaaa ttttaaattc cagagttagg ttgctctatt aaatccataa
     2041 taagactatc atctactagt ctcaatagtt cgttctcaac accttcaaaa gtgatggtat
     2101 atccattaag gtctccacct tctgt
//
LOCUS       DATGSI010000228         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_207293, whole genome shotgun sequence.
ACCESSION   DATGSI010000228 DATGSI010000000
VERSION     DATGSI010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207293"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VK031_03480"
     CDS             <1..221
                     /locus_tag="VK031_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HLS53008.1"
                     /translation="KLIKVLNQLVEGGNTVIVIEHNLDVIKTADHIVDLGPEGGDKGG
                     MVVGIGTPEEIAKMEESYTGEFLKRVLD"
     gene            286..1242
                     /locus_tag="VK031_03485"
     CDS             286..1242
                     /locus_tag="VK031_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HLS53009.1"
                     /translation="MKITVLEPLGVSEERLREIASPLTEAGHQLEIFNEWTPDIHKRK
                     ERVKDTDILVIANSPLEGDVIRAAKNLKMISVAFTGVDHVDLEACKERGILVSNSAGY
                     STPAVVELAFGLMISLLRNMVPLDQVTRNGGTMAGYSQRELRGRTIGVVGTGAIGSGV
                     AEVALAFGCDVLAYNRSEKAHLKAKGVTYVDLDTIFEESDIVTIHLPLIDETEGLVDE
                     TRLRMMKKDAILINVARGPIVDNEALAKVLNEGVIAGAGIDVFDMEPPIPEDYSLLKA
                     RNTLLTPHIGFASEEAMLRRADIVFKNIECWLKDKPQNVIKF"
     gene            complement(1285..>1550)
                     /locus_tag="VK031_03490"
     CDS             complement(1285..>1550)
                     /locus_tag="VK031_03490"
                     /inference="COORDINATES: protein motif:HMM:NF022969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HLS53010.1"
                     /translation="MNYLPEEDLVLVPGGTLLTDTPEEGRALAMLMARHTIHNIQPDL
                     DILMCGRRIYSVDPDSLMFASQVVAIEFQTIAAANNYWRDCYY"
BASE COUNT          525 a          240 c          384 g          401 t
ORIGIN      
        1 ataagttgat caaggtatta aatcaactgg tggagggcgg aaacacagtc atagtcattg
       61 agcataacct agatgtaatt aagactgctg atcatatagt ggaccttgga cctgaaggtg
      121 gagacaaggg aggaatggtg gtaggcatag gtacgcctga ggaaatagca aagatggaag
      181 aatcatatac aggagaattt ttgaaaaggg tccttgatta agcaggttta agtataaaat
      241 ttgttaggtt aataaagtca aataagttta atggggggat tcaatttgaa aataacagtc
      301 ttggagccat tgggagtcag tgaggaaagg ttaagggaaa ttgccagccc tttgacagag
      361 gcagggcatc agttggaaat ttttaatgaa tggactccag atatccacaa gcggaaggaa
      421 agggttaagg acacagatat cttagttata gccaactcac ctttagaagg ggatgttata
      481 agggctgcaa aaaacctaaa aatgatatct gtagccttta caggggtaga tcatgtagat
      541 ttagaggcct gtaaggaaag agggatcctg gtgtctaatt ctgcagggta tagtactcct
      601 gcagtagtag aattagcctt tggtttaatg atttccctgc tgagaaatat ggtacccttg
      661 gaccaggtaa caaggaatgg ggggaccatg gcaggttata gccaaagaga actaagggga
      721 agaaccatag gagttgtcgg aacaggggct ataggaagtg gagtggctga agtggcccta
      781 gcctttggat gtgacgtact tgcatacaat agaagtgaaa aggcgcatct taaggctaaa
      841 ggtgttacct atgtagattt agataccata tttgaagaaa gtgatatagt aacaatccat
      901 ctacccttaa tagatgagac tgaaggattg gtggatgaaa ccagattaag aatgatgaaa
      961 aaggatgcta ttttaataaa tgtggccaga ggacctatag tagataatga agctttagcc
     1021 aaggtattaa atgaaggcgt gatagcagga gcaggtattg atgtgtttga tatggagcca
     1081 ccaataccgg aagactattc cctgcttaag gcaagaaata cattattaac tccccacata
     1141 ggatttgcat cggaggaggc catgctaagg agagcagaca tagtatttaa aaatatagaa
     1201 tgttggctaa aagataagcc acaaaatgta ataaagtttt gataaagaaa ccacctttac
     1261 ccacatggat aaaggtggtt tttattaata ataacaatct ctccaataat tgttagcagc
     1321 ggctatagtt tgaaactcaa tagccactac ctgactggca aacattaggc tatctggatc
     1381 aacgctatag atcctgcgtc cacacattaa aatatctaaa tcaggctgaa tattgtggat
     1441 agtatgtctg gccataagca tagccaatgc tcttccctct tctggtgtat cagtaagcag
     1501 ggtaccgcct gggactagga ctaaatcctc ttcaggtaaa taattcataa
//
LOCUS       DATGSI010000229         5621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_207304, whole genome shotgun sequence.
ACCESSION   DATGSI010000229 DATGSI010000000
VERSION     DATGSI010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207304"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..788)
                     /locus_tag="VK031_03495"
     CDS             complement(177..788)
                     /locus_tag="VK031_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HLS53011.1"
                     /translation="MRLGRDFFTRDTARVAKELLGKVIVLNKDGRTFKGRIVETEAYT
                     GDNDDAAHFHKGITERTRVIDEVGGHIYIYNIYGIYQCFNIVAEKRKVHGGALIRGIE
                     PIEGIEEMYKNRYKKDYLNTSKRELINLTNGPAKFVMAFGITKDEFYGRDLVTDPEIW
                     VENRPSLAEEEIVTTTRVNIDYAKSRDYLLRFYIKDNPYVSKK"
     gene            937..1638
                     /gene="thyX"
                     /locus_tag="VK031_03500"
     CDS             937..1638
                     /gene="thyX"
                     /locus_tag="VK031_03500"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HLS53012.1"
                     /translation="MKIKKVLDKGYVRLVDSLGSDLTIVNAARVSYAKESSQLDDKDI
                     RLIKFLAREDHTSPFRHAMLQFEIYAPLMVARQWWKYIVGSAHQEAIGDSLNAWNESS
                     RRYITEDAEFYYPTEEEWRSKPDHSKQGSGEPVEWELGKEYTEKLMEYAEEGLKLYNQ
                     AMEDGICAEQARLFLPAYGLYVRWYWTASLQSVAHFIQQRIEHDAQKEIQVYAQAVLD
                     LAKEIFPISLQALFD"
     gene            1681..2013
                     /locus_tag="VK031_03505"
     CDS             1681..2013
                     /locus_tag="VK031_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HLS53013.1"
                     /translation="MKDLLSKQCIPCSIDSEPLTIEEVNLYLEKIDQDWKLAEDGRIE
                     KNFKFKNFKEALNFTNLVGELADQEGHHPSLILAWGLVRVRIWTNNIQGLHENDFILA
                     AKIDQLKR"
     gene            2099..3664
                     /locus_tag="VK031_03510"
     CDS             2099..3664
                     /locus_tag="VK031_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HLS53014.1"
                     /translation="MVIWGDFLSRNKFIQGSIIMILINIFTRTIGFAYEVLLSKHLGA
                     EAMGWFQMVMSVLMVFLVFTISGIPSSITKLVAEEHFKGNRYNVEKIYKSAIFFNLIL
                     ANLLCLLLLNSASFISEKVLGKEDLLLGIYLLIPAILIISLSNILRAYFYGIQNMIIP
                     SVSQTIEHFSRFVLVIGAILYFSPIKPIYGTMLAILGISIGEFFDLAWSLIHKRKLTK
                     IPPAFNPNGWGYGKTLKKLLLLSLPLTLSSFFNVGLNFLNSILIPSRLVSAGYTNSLA
                     MASFGRITGMAMPLINLPFLVTSSLVVNIIPSLSEQLSLNKRQNMKSDIYLAVKITLL
                     ISIPLTFLYVMLAEPISIFLYKDLLAANYIKIMGYGTSFLALQHTFSGILFGLNKQNQ
                     ATSYRMLAMLIRVFLIYTLVGNPRVEIYGYFIAFYISNILIITLDFFTLKRTIEINFN
                     YRDLILKPVLASLFMTVYLKLTVFDLDNLKYVDPLAFAFSLFVAFLSYVFILIVTKAL
                     PKDGLKEIFNKQL"
     gene            complement(3787..4122)
                     /locus_tag="VK031_03515"
     CDS             complement(3787..4122)
                     /locus_tag="VK031_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLS53015.1"
                     /translation="MERQKALFADLALLLVAKIWGSGFIVNKNTLDYLSPIYIIILRF
                     SLASFLLGLIFYKRLITITGADLKAGVIIGILLFCAFLFQTIGLQYTTVSKSAFITAS
                     NVVMVPFIR"
     gene            complement(4123..4320)
                     /locus_tag="VK031_03520"
     CDS             complement(4123..4320)
                     /locus_tag="VK031_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HLS53016.1"
                     /translation="MEKTICPLCGGDNNCENRNACWCYKVVVPKELLELVPDDKRGKS
                     CICKSCIDKYNRKKTIKGDDS"
     gene            4459..>5621
                     /locus_tag="VK031_03525"
     CDS             4459..>5621
                     /locus_tag="VK031_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HLS53017.1"
                     /translation="MIVGLDMGGTNIDGVIIQDGKIIRTVKNPTDRDNLFESIWNSLE
                     ELLADIEKEKINRINLSTTVSTNAIVENKTCPVGMIIQSGPGIPDSFLACGNSNVFVS
                     GYIDHRGKVIERYKKAEIRRAISNFKEANVDSVAIVTKFSTRNPDLEKEIKNLLEKDF
                     SPITMGHTVSGKLNFPRRVFTSYLNSAVYNVFNNFSNNIKKSLESKGITAPVYLLKAD
                     GGTMNIKTAEEKPVETILSGPAASFMGINAMLPVDKDAILLDIGGTTTDIFFLADGVP
                     LFEPLGIKIDKYKTLVRSIYSQSIGLGGDSAVKLMEGRIKIGPERKGNPYAFGGPVAT
                     PTDAMIVLKQIDKDQILKYISNNQSGIKYGQVEKRAYKAMKELGKDIKLSAKET"
BASE COUNT         1810 a         1028 c          926 g         1857 t
ORIGIN      
        1 agaaacaata aatatataaa ctgctatggt tgtatattta atattccact ttatcctcat
       61 aggcccactc cctctaattt aattcctttc aatctacctc taatattaat gttatcaaaa
      121 aaagtagtgt ctaaccacta ctttccataa ttatcacata tttcctaatc aaatctctat
      181 ttcttggaaa cataaggatt atcttttata taaaacctta acaaatagtc cctacttttt
      241 gcataatcaa tgttaaccct ggttgttgtt acaatttctt cctcagccag agaaggtcta
      301 ttctctaccc aaatttcagg atctgtaacc aagtccctac cataaaactc atctttcgta
      361 atgccaaaag ccattacaaa tttggccggt ccgttagtta aatttattag ctctctttta
      421 ctagtattta aataatcctt tttataacga tttttgtaca tttcttctat tccttctata
      481 ggctctatgc ctcttattaa tgctcctccg tggacctttc ttttttctgc cactatattg
      541 aagcattgat atataccgta tatattgtaa atataaatat gcccacctac ttcatctatt
      601 accctggttc tttccgtaat ccccttatga aaatgggccg catcatcatt atcaccagta
      661 tatgcctcag tttccacaat tcttccctta aaggttctac catctttatt aagtactatt
      721 accttgccca gtaattcttt agctaccctt gcagtatctc tggtaaaaaa gtctctgcct
      781 agtctcataa acatccccta ttctttttta atgcttattt aataatatac ctctttttct
      841 aggattatag tctagttagt ctaaatttca aataattatt attaaaaatt atgggtatca
      901 gagattatga atatgtaatt ttaaggaggt taaaatatga agataaagaa ggtattagat
      961 aaaggttatg taaggttggt tgattctcta ggatctgatt taaccatagt aaatgccgcc
     1021 agggtttcct atgccaagga atcaagccag ttagatgata aagatatacg attaattaag
     1081 tttctagcca gggaggacca tacttccccc tttagacatg ccatgttgca atttgaaata
     1141 tatgctcccc taatggttgc taggcaatgg tggaagtata tagttggttc agcccatcaa
     1201 gaggctattg gagattcttt aaatgcctgg aatgaatcta gcagaagata tataactgaa
     1261 gatgcagaat tttattatcc tactgaagaa gaatggagga gtaaacctga tcactccaag
     1321 caaggtagtg gtgagccagt tgaatgggaa ctgggcaaag aatatactga aaaacttatg
     1381 gagtacgctg aagaaggctt aaaattatat aatcaggcaa tggaagatgg catatgtgct
     1441 gaacaggcca gactatttct accggcctat ggcctgtatg ttagatggta ttggacagcc
     1501 tcccttcagt cagtggccca ttttatccag caaagaatag aacatgatgc acaaaaagag
     1561 atccaagttt atgctcaggc tgtcttggat ctagccaaag aaattttccc aatctctctt
     1621 caggcattat ttgattgatt tatgactaat tataagtaaa tctagtctgg aggtgttagt
     1681 ttgaaagact tactatcaaa gcaatgtatt ccctgtagta tagattcaga accattaaca
     1741 atagaagagg taaacttata tttagagaag attgatcagg attggaagct agcagaagat
     1801 ggtagaatcg aaaaaaactt caaattcaaa aactttaagg aggccctaaa ctttacaaat
     1861 ttggtgggag aattagcaga ccaggagggc caccacccta gcctaatctt ggcctggggt
     1921 cttgttaggg taagaatctg gaccaataat attcaaggat tacacgaaaa cgactttatc
     1981 ctggcagcaa aaattgatca gttaaaaaga taagcatcct gtaaggatgc ttattttcat
     2041 cccgttacca taaataaatt atagaatgat atatttttct cctcatataa attaataaat
     2101 ggtaatttgg ggtgattttt tgtccagaaa caaatttatt caaggctcaa ttataatgat
     2161 attaataaat atatttacca gaactattgg atttgcctat gaagttcttt tatctaaaca
     2221 tttaggggcc gaggccatgg gctggtttca aatggttatg tctgtcctta tggttttttt
     2281 agtctttaca atatcaggta ttcctagctc tattactaag ctggtggctg aggaacactt
     2341 taaaggaaac aggtataatg tggaaaaaat ctataaatct gctatttttt ttaaccttat
     2401 tttagcaaat cttttatgtc tcctcttatt gaattctgcc agctttatat ccgaaaaagt
     2461 tttaggcaag gaggatcttt tattgggcat ttatctatta atcccagcca tacttatcat
     2521 atctctaagc aatattttaa gggcctattt ttatggtata caaaatatga ttatacctag
     2581 tgtttctcaa acaatagaac actttagccg ttttgtcctt gttattggtg ctatccttta
     2641 cttttccccc ataaagccca tctatgggac aatgttggct attttaggta tcagtattgg
     2701 agaattcttt gatctggcct ggtccttgat tcataaaaga aagttaacca aaattccccc
     2761 ggcctttaat cctaatgggt ggggatatgg caaaacctta aaaaagttgt tacttctttc
     2821 tttgccttta accctttcat ccttttttaa tgtgggctta aactttttaa actctatttt
     2881 gataccttcc aggcttgtat cagctggcta tacaaacagc cttgccatgg ccagttttgg
     2941 tagaattaca ggtatggcca tgcccctaat taacctaccc tttttggtta cctccagcct
     3001 ggtggtcaac atcattccca gcctttcaga gcaactgagt cttaataaaa ggcagaatat
     3061 gaagtcagat atatatttgg ccgtcaaaat taccctgctt atatctattc ctttaacctt
     3121 tttgtatgta atgctggcag agcccatatc aatattttta tataaggatc ttctagctgc
     3181 caattacata aaaattatgg gttatggcac aagcttttta gccttacaac ataccttctc
     3241 aggtatcttg tttggcctaa ataaacaaaa tcaggcaacc agctatagga tgttggccat
     3301 gctgattagg gtctttctta tctataccct ggttggaaat cctagggttg aaatttatgg
     3361 ctactttata gccttttata tctcaaatat tttaataatt acattagact ttttcacctt
     3421 aaagaggacc attgaaatta actttaatta cagggacctt atcttaaaac ctgtcctagc
     3481 ctcccttttt atgactgttt acctaaaatt aactgttttt gatttagaca atttaaaata
     3541 cgtagatcct ttagcctttg ccttttcact ttttgtagct tttttatctt atgtttttat
     3601 cttaatagtt acgaaagcac tgccaaagga tggtttaaaa gaaatattta acaagcagtt
     3661 ataatgaact cagtttatcc tatttaagag gtaactttcc tctttccaag agctaagcct
     3721 acttctgcta aaaatatggc cacaagtatt attcctgaac ccaagatcag cctatttgta
     3781 ataggctcac ctaataaaag gtaccatcac cacattactg gctgttataa aggccgattt
     3841 actaacggta gtatactgta gacctatggt ctgaaaaaga aaggcacaaa ataataagat
     3901 tcctataatt accccagctt ttaaatctgc ccctgtaatt gttataagtc ttttatagaa
     3961 aatcaggccc aacaaaaaac ttgctaagga gaaacgtaaa attattatat aaatagggct
     4021 taaataatcc aaggtatttt tatttactat aaatccactt ccccaaattt tggcaactaa
     4081 caatagggcc aaatctgcaa ataaggcctt ttgcctttcc atctagctgt catccccttt
     4141 aatagttttt ttcctattat acttatctat gcaggatttg caaatacaac ttttacccct
     4201 tttatcatct ggtacaagtt ctaaaagttc ctttggaacc accaccttat aacaccaaca
     4261 agcattccta ttttcacaat tattgtcccc tccacatagg ggacatattg ttttttccac
     4321 agataattcc tcctaatcca ttagtctttc ctagtatcac attataccat atgactaatc
     4381 agctctttct aaaacttgat aaattttccc atctctagat ataatgtaaa tagatagtaa
     4441 gcaaagggag tgttttaagt gatagtaggt ctagatatgg gtggtactaa tattgacgga
     4501 gtcattatcc aagatggtaa aataataaga actgttaaaa acccaactga tagggataat
     4561 ttgtttgaat ccatatggaa cagcctagaa gaattacttg cagacattga aaaagaaaaa
     4621 atcaacagaa ttaacctaag taccacagta tccaccaatg caattgttga gaataaaacc
     4681 tgtccagtag gtatgattat tcaatctggt cctggaatac cagatagttt tttagcctgt
     4741 ggcaattcca atgtatttgt ttctggatat attgatcaca ggggtaaagt tatagaaagg
     4801 tacaaaaaag ctgagataag aagagccata agtaatttta aagaagctaa tgttgactca
     4861 gttgctatag taactaaatt ttctaccagg aatccagacc tggaaaaaga aattaaaaac
     4921 cttttggaaa aagatttttc tcctattaca atgggtcata ctgtatctgg taaattaaac
     4981 tttcctcgca gagtttttac atcttatttg aattcagctg tctataatgt ttttaataac
     5041 ttctcaaata atataaaaaa atctttggag agcaaaggga taactgctcc agtttacctg
     5101 cttaaggcag atggcggaac catgaatatt aaaacagcgg aagaaaaacc tgttgaaaca
     5161 attttatcag ggccagctgc cagctttatg gggatcaatg ccatgctccc agtagataag
     5221 gatgcaatcc ttctagatat tggaggcact actacagata tatttttcct agcagacgga
     5281 gttcccctat ttgaaccctt aggaattaaa atagataaat ataaaacctt agttagatct
     5341 atctatagtc aatccatagg ccttggagga gatagtgctg ttaaattaat ggagggcagg
     5401 attaaaatag gtcctgaaag aaagggaaat ccctatgctt ttggtgggcc tgtggcaact
     5461 cctacagatg ccatgatagt gctaaagcaa atagataagg accaaatatt aaagtatatc
     5521 agcaataatc aatcaggtat taaatatggc caagttgaaa aacgggcata taaggctatg
     5581 aaagaactag gaaaagatat aaaactttca gccaaggaga c
//
LOCUS       DATGSI010000230         1178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_585060, whole genome shotgun sequence.
ACCESSION   DATGSI010000230 DATGSI010000000
VERSION     DATGSI010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585060"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VK031_03530"
     CDS             complement(<1..210)
                     /locus_tag="VK031_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53018.1"
                     /translation="MYEIIKALDRLDKVKTGRRRCYEDKTGDTIIHIGGDSIELVHKL
                     FSEESLEITKDFRGVSITVDNPEFLA"
     gene            complement(251..628)
                     /locus_tag="VK031_03535"
     CDS             complement(251..628)
                     /locus_tag="VK031_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53019.1"
                     /translation="MKDKRAYVELQRYKKATEFLINREAELVTREEFEKRFSDQYSKS
                     FESLIDLLQVVKDKTDTESGNFWFTNLVEIKGIFPLIDNLISDNTRLKGSFQNLYKLY
                     REQIDLNIELQSKIDRIVTEELK"
     gene            complement(618..1094)
                     /locus_tag="VK031_03540"
     CDS             complement(618..1094)
                     /locus_tag="VK031_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53020.1"
                     /translation="MKELEIKNILPYLPYEVEVYFEGEEFPHEIVGLDMSSRPLHILS
                     PWGQFGMSSLEGAKLVLRPLEDIFKEINHRGKIVTPHREISKILNGGKYSDSFNIIEY
                     NEYGSLRINFGDHTTGYSINLETIPVLEKLYELHFDVRGLIDQGLACNINDFLYEG"
BASE COUNT          341 a          231 c          149 g          457 t
ORIGIN      
        1 cgcaagaaac tctgggttat ctaccgtgat agaaactcct ctgaaatctt ttgttatttc
       61 taaactctct tctgaaaaaa gtttgtgaac taattctatt gaatctcccc ctatgtgtat
      121 tatagtatct cctgttttgt cctcgtaaca cctgcgccta cctgttttaa ctttgtctaa
      181 cctgtctaaa gcttttatta tttcgtacat atctaattat tttttttgtt tacactacaa
      241 atgtaataac ttattttaac tcttctgtca caattctgtc aatcttagat tgaagttcta
      301 tgttcaaatc tatttgttct ctgtaaagct tatataagtt ctgaaaactg ccttttaatc
      361 gagtattatc tgaaataaga ttatctataa gagggaaaat tccttttatc tctactaagt
      421 ttgtaaacca gaagttacca gattcagtat ctgttttgtc tttcactacc tgtaataagt
      481 ctattaagga ttcaaagctt ttactgtact ggtctgaaaa ccttttctca aactcttctc
      541 tcgttacaag ttctgcttct ctgttgatta aaaactctgt tgctttcttg tatctctgta
      601 gctctacata agctctttta tccttcatat aaaaaatcat taatattgca agctaatcct
      661 tgatcaatta atcctcttac gtcaaaatgt agctcgtaca atttctccaa cacaggaata
      721 gtttcaaggt ttatagaata accagtagta tgatctccaa aattaattcg taagcttcca
      781 tattcattat actctataat attaaaactg tcagagtact ttcctccatt taatatctta
      841 cttatctctc tgtgcggagt tactattttc cccctgtgat ttatttcttt gaaaatatct
      901 tctagaggtc tgaggactaa tttagcgcct tctaaagaac tcataccgaa ttgtccccat
      961 ggagataata tatgcaaagg tctggaagac atatccaatc ctactatctc atgagggaac
     1021 tcttcccctt caaaatacac ctctacctcg taaggtaggt atggcaatat attctttatt
     1081 tctaattctt tcataactat ttacttttaa aaaggtgcat cattaagtcc aaattctaag
     1141 tttggtttaa tagctgattc actatcattt tgtttgaa
//
LOCUS       DATGSI010000231         1814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_678526, whole genome shotgun sequence.
ACCESSION   DATGSI010000231 DATGSI010000000
VERSION     DATGSI010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678526"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VK031_03545"
     CDS             <1..638
                     /locus_tag="VK031_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53021.1"
                     /translation="WTPLFEIIGSLILGAIIGFLLSYIAPRTRNRDELLSVVLAAIFL
                     GMGMANQIGLSPLLTCMMIGAVLINLMQNANRVFNLITDFTPPIYLLFFTIAGASLEL
                     GVLTKVGLLGIGYIIARAIGKIVGATWGAKRVNSDQKVVKYLGMSILTQGGISIGLSM
                     IVARELPQFSESIITVILFSVLIFEIAGPILAKIAIQKAGEEDGILREKGK"
     gene            671..1012
                     /locus_tag="VK031_03550"
     CDS             671..1012
                     /locus_tag="VK031_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53022.1"
                     /translation="MNVLIVVLNKIEYLDDILDGFIDVGVSGATIIDSQGMGSAITNG
                     GMGHEPFFGAIRSFLDNSRPYNKTIFTVIEDEEVLDKAIKMVKDILGDMEKPGVGMMF
                     TMPVGQVHGIN"
     gene            1079..1369
                     /locus_tag="VK031_03555"
     CDS             1079..1369
                     /locus_tag="VK031_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53023.1"
                     /translation="MFEDFMTAEILTTFAGLTTAVIVIVQFTKPFIKEKLGDSFVRLY
                     TFAIALILTFIFARQGSKMEGIIVTIINAMMITIASMGGYEMIADPMAQKKR"
     gene            1432..>1814
                     /locus_tag="VK031_03560"
     CDS             1432..>1814
                     /locus_tag="VK031_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HLS53024.1"
                     /translation="MQKVIGFIGYGNMARAMVRGLISSEIISAENIIASRKHISQKGR
                     QKDELNIYITDNNLEVARKSDYIILATKPNIYKQILKKIKNEIKDNAVVIGIGAGISS
                     KFLAKNLKPGTKYVRAMPNTPAMVG"
BASE COUNT          646 a          242 c          403 g          523 t
ORIGIN      
        1 tatggacacc tttatttgaa ataataggct ctcttatttt aggtgccata ataggatttc
       61 tccttagcta tatagcacca agaactagaa atagggatga attattatct gtggttttag
      121 ctgctatttt tctagggatg ggcatggcaa accaaatagg cttgtcacca ttgttaactt
      181 gcatgatgat tggagcagta ttaattaatc taatgcagaa tgcaaatagg gtattcaatc
      241 taattacaga ttttacccca cctatatatt tgttattttt tacaatagca ggagccagct
      301 tagaattagg tgttttaaca aaggtaggcc tattaggtat aggttatata atagctagag
      361 cgatagggaa gattgtaggt gcaacttggg gagcaaaacg tgtaaatagt gaccaaaagg
      421 ttgtaaaata tttgggtatg agtatattga cacaaggtgg tatttccatt ggtctatcta
      481 tgattgtagc cagagaatta ccacaattta gtgaatccat cataacggtt attctgttta
      541 gtgtgcttat atttgaaatt gccggcccaa tattggcaaa gatagccatt caaaaagccg
      601 gtgaagaaga tggaatatta agggaaaagg gaaagtaaag tagctagtta ctaaaaagga
      661 tggtgataaa atgaatgtgt taattgtggt tcttaataag atagaatatc tagatgatat
      721 actagatggt tttattgacg taggagtaag tggggccacc ataatagata gccagggaat
      781 gggctcagcc ataaccaatg gtggaatggg tcatgaacct ttttttgggg ccatcagatc
      841 tttcttagat aactcaaggc cctataacaa aactatattt acagtaatag aggacgaaga
      901 ggttttagat aaggctataa agatggttaa ggacatactt ggagatatgg aaaagccagg
      961 agtgggaatg atgtttacaa tgccagtagg gcaggtccat gggataaact aacaatcatc
     1021 ataatccccc tgattaaggc ataatatcat agtagatatt ataagggggg gatgatttat
     1081 gtttgaagat tttatgacgg cagaaatatt aactacattt gcagggctta caacggccgt
     1141 aatagtgatt gtgcagttta ccaaaccttt tataaaggaa aagctaggag attcctttgt
     1201 tagactttat acatttgcta ttgccttaat attaactttt atttttgcaa ggcagggtag
     1261 caaaatggaa ggcataattg ttacaataat taatgcaatg atgataacta tagctagcat
     1321 gggtggttat gaaatgatag ctgatcctat ggcccagaaa aaaagatgaa agattctgtt
     1381 acaagataga cctcctatga tataattatt taaaataggg ggcgggtaaa aatgcaaaag
     1441 gtaataggat ttatagggta cggaaatatg gctagggcca tggtgagagg cctaataagc
     1501 tctgaaataa tttcggcaga aaatattatt gcatctagaa agcatatatc tcaaaaagga
     1561 agacaaaaag atgaattaaa catatatata acagataata atctggaggt agcccgaaaa
     1621 tcagattaca taatattggc tacaaagcct aacatataca agcaaatact gaaaaagata
     1681 aaaaatgaga taaaggacaa tgctgtggta atagggatag gtgcagggat tagctctaag
     1741 tttttagcta aaaatctaaa accaggaacc aaatatgtaa gggctatgcc aaatacacca
     1801 gccatggtag ggga
//
LOCUS       DATGSI010000232         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_440633, whole genome shotgun sequence.
ACCESSION   DATGSI010000232 DATGSI010000000
VERSION     DATGSI010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440633"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VK031_03565"
     CDS             <1..644
                     /locus_tag="VK031_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HLS53025.1"
                     /translation="VNPTNIMGASKRICEMIIQSINTHSETEFVAVRFGNVLDSNGSV
                     VPLFKKQIEEGGPVTVTHEDVIRYFMTIPEAVQLVIQAGAMAKGGEIFILDMGEPVKI
                     LDLAKDLIRFSGFEPEVDIPIEIIGLRPGEKLYEELLLAEEGVLETKHDKIFVGQPTF
                     SDYRLLLKSLDQVQELIEKGTNEELIEYIKAMVPTFKKPAEINEPALKESTHI"
     gene            662..1336
                     /locus_tag="VK031_03570"
     CDS             662..1336
                     /locus_tag="VK031_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HLS53026.1"
                     /translation="MEEISLQELFSILRKWSWLILTLLIVSIALSGIVSIFILKKQYE
                     ANTTLMVGKPRDYHGEGNIDYNELVLNQKLVSTYGELIKTRRVTDSVIRNLRLPISNK
                     DFISKVSVSLVKDTEIIKIQVTDEDPKLAARMADETARVFIEAVKDIWKVENVQIIDK
                     AQVPKHHVRPRVKLNIAISAVLGIMIGVFVAFLIEFLDNTIKTSEDVEKYLGIGVIGE
                     IPEYEN"
     gene            1326..>1705
                     /locus_tag="VK031_03575"
     CDS             1326..>1705
                     /locus_tag="VK031_03575"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HLS53027.1"
                     /translation="MKIRNIWGETVRNIGLITQTNPKSPMAEAFRTLRTNIQFANIDE
                     EIESMVITSGQPSEGKSTVAINLALTIGQGGKRVLLIDCDMRKPTIHKTFRISNLFGL
                     TNILMGTKTLEEVLYSQEERLGSV"
BASE COUNT          600 a          237 c          381 g          487 t
ORIGIN      
        1 cagttaatcc tactaatatt atgggagcca gcaagagaat atgtgaaatg ataatccaat
       61 caattaatac acattctgaa acagaatttg ttgcagttag atttggaaat gttttagaca
      121 gcaacggtag cgtggttccc ttatttaaaa agcaaataga agagggtggg cctgttactg
      181 taactcatga agatgtaata aggtatttta tgaccattcc ggaagcagtc cagctggtta
      241 ttcaggctgg agccatggct aaaggaggag aaatcttcat attagacatg ggtgagccag
      301 ttaaaatctt ggatttggcc aaggatctaa ttagattttc aggttttgag cctgaggttg
      361 atataccaat tgaaattatt ggcctaaggc ctggagaaaa actgtatgaa gagctattat
      421 tggcagaaga gggggtcttg gagaccaagc atgataagat ttttgtagga caacctacct
      481 tttcagatta taggctgtta ttgaaatctc tagatcaggt acaggaactg atagaaaagg
      541 ggacaaatga ggaattgatt gaatatatta aggctatggt gccaaccttt aagaaacctg
      601 cagagattaa tgaaccggcc ttaaaagaat caacacatat ttaatatagg gggtattaaa
      661 tttggaagaa attagtctac aggagttatt ttctattttg agaaagtggt cctggttaat
      721 attaacttta ttaatcgttt ctatagcctt aagtggcata gttagtatat ttattcttaa
      781 aaaacaatat gaagccaaca caaccctaat ggttggaaaa cctagggact atcatgggga
      841 aggaaatatt gattataatg aactggtgct aaatcaaaag ttggtttcca catatgggga
      901 acttattaag actagacggg tcaccgacag tgttataagg aatttaagac tccccatatc
      961 caataaggac tttatatcaa aggtatcagt cagtttggta aaagataccg agataataaa
     1021 aatccaggtt acagatgagg atcccaaatt ggcagccagg atggcagatg agacagccag
     1081 agtatttatt gaggctgtaa aagatatttg gaaggttgaa aatgtgcaaa ttatagacaa
     1141 ggcccaggta cctaaacacc atgtaagacc aagggtgaaa ctaaatattg ctatttcagc
     1201 tgttctagga ataatgatag gagtatttgt ggcatttcta atagagtttt tagataatac
     1261 tataaagact tctgaagatg tggaaaaata tttaggtatt ggtgtaattg gagaaatacc
     1321 agaatatgaa aattaggaat atatggggtg aaactgtgag aaatatagga ctcattactc
     1381 aaactaatcc taagtctccc atggcggagg cttttaggac tttaagaact aatatacaat
     1441 ttgccaatat tgatgaggag atagaaagta tggtaattac ctccggtcaa ccaagtgagg
     1501 gtaaaagtac tgtggctata aatcttgcct taacaatagg acaaggaggt aaaagggtac
     1561 tgctaattga ttgtgatatg agaaagccaa ccatacataa aacctttaga atatctaatc
     1621 ttttcggtct tacaaacata ttaatgggca ctaaaacctt ggaggaggtg ctctatagtc
     1681 aggaggaaag attaggaagt gttta
//
LOCUS       DATGSI010000233         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_440634, whole genome shotgun sequence.
ACCESSION   DATGSI010000233 DATGSI010000000
VERSION     DATGSI010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440634"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      <1..95
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            203..874
                     /locus_tag="VK031_03580"
     CDS             203..874
                     /locus_tag="VK031_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HLS53028.1"
                     /translation="MKILYGIGTGPGDAELLTLKAVKTIERSDVIFAPNNKGKNMALD
                     TAKEYVKDKRIVLIDFPMGAVTKKDYEEAARIINREIPKDGQGAFLTIGDPMIYSTFI
                     YLMEEIERKSLGIQVNIIPGIPSFVAAAAAAKLPLTVKGDNFLLCDEFNGDLLKNINS
                     ISILKSLRGKEEILNKLEKEKFDYTYVRRISLEEEGIMKDREKILSDRNYMSLILARK
                     TKEEE"
     gene            875..1837
                     /locus_tag="VK031_03585"
     CDS             875..1837
                     /locus_tag="VK031_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLS53029.1"
                     /translation="MKKRLISIVLIFVLVSSILSACSKETLKEAEEANKDGESITVTD
                     DLGREVTIEKNPEKVVVLFSSFIDIWVKSGGKLVGMTEDQLVHDVKGLDGVEGVGRTG
                     GLSLEKILSLEPELVILSVGTTEQRELVPILEENNVKVLALEYILKDDYFRIAKLFSE
                     INDSKDLYEENVATVEKQIEEVIERVPDEEIRVFIMFASSKSISARGSDSTLGEMLAD
                     LKTINIADEASELLNDKNFSLEKVIEEDPDFIFVQTRGSDMEAVNERIKKDVESNPAW
                     ASLSAVKNDRYIMLPKDLYLYKANDRYGEAYENLAKILYPDIFN"
     gene            1852..>2338
                     /locus_tag="VK031_03590"
     CDS             1852..>2338
                     /locus_tag="VK031_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HLS53030.1"
                     /translation="MGKKLTQSSKYNFLVILILIIVFITSFFLSVGNGAVKLGPREIV
                     KSIFLRQENTNYQIIWNVRLPRTLVASLVGICLSLSGAMLQGIMKNSLAGPNIIGVSS
                     GAGLFGLIILILYPEYYYLVPLGAFFGALIATLLIYILAWKEGVQPSRLILAGVAVSS
                     IF"
BASE COUNT          829 a          316 c          520 g          673 t
ORIGIN      
        1 acgaaattag cgtataccac tgggtaaaac cgggaaggtg ctaaaagtag ggtgattcac
       61 aagtcaggag acctgcctaa tagctgtaag tagtaagtaa ccttcggagg gaagggaagg
      121 cttgtttttt tatgcgtaga agtatatcta tccctaatct ttgaagatta gggatttttt
      181 atttactaaa aggagggaaa aaatgaaaat tttatatgga atagggacag gaccagggga
      241 tgctgaactg ttaaccttaa aagcagtaaa aaccattgaa aggtcagatg taatatttgc
      301 accaaataat aagggaaaaa atatggcctt ggatactgca aaagaatatg taaaggacaa
      361 gaggatagtc ttaatagact ttcccatggg agcagtgact aaaaaagact atgaagaagc
      421 tgcaaggata atcaataggg agattccaaa agatggacaa ggggcctttt taaccatagg
      481 agatcctatg atatatagca cctttatcta tcttatggag gaaatagaga ggaaaagctt
      541 aggtatacaa gtaaatataa ttccaggtat accctctttt gtagcagcag ctgcagcagc
      601 taaattaccc ttaactgtaa agggagacaa tttccttttg tgcgatgaat ttaatggaga
      661 cttactaaaa aatattaact ccatatccat cctaaaaagt ttaagaggaa aagaagaaat
      721 cctaaacaaa ctggagaaag aaaagtttga ttatacatat gtaagaagga ttagcctgga
      781 ggaagagggc attatgaagg atagagaaaa aattttatca gatagaaatt acatgtccct
      841 aatattagca agaaagacta aggaggaaga ataaatgaaa aaaagattaa tatcaattgt
      901 acttatattt gtgttagtgt catctatact gtccgcctgt agtaaggaaa cgttgaagga
      961 agcagaagaa gcaaataaag atggggaatc cataacagtt acagatgatt tggggaggga
     1021 agttactata gaaaagaacc cagaaaaggt agttgttctc ttttcctctt ttattgacat
     1081 atgggtcaaa agtggtggaa aactggtagg aatgacagag gaccaactgg ttcatgatgt
     1141 gaaagggcta gatggggtag agggagtagg aaggactgga ggcttaagcc tggaaaagat
     1201 tttgtccctg gaaccggaac tagttatttt aagtgttggt acaacagaac agagagagtt
     1261 ggttcccatt ttagaagaga ataatgtaaa ggttttggcc cttgaatata ttttaaaaga
     1321 tgattacttt agaatagcca aattattttc agagataaat gacagcaaag atttatatga
     1381 agagaatgtg gctacagtgg aaaaacaaat agaggaagta atagaaagag tacctgatga
     1441 agaaataagg gtattcatca tgtttgcaag ttctaagtct atttcagcta gaggatctga
     1501 ttctacttta ggtgaaatgt tagcggacct aaagactatt aatatagcag atgaggctag
     1561 tgagctttta aatgataaaa actttagctt ggaaaaggta atagaagagg atccagattt
     1621 tatatttgtt caaactagag gcagtgatat ggaagcggtg aatgaaagga taaagaagga
     1681 tgtggaatca aatcctgctt gggcttcttt gtctgctgta aaaaatgata gatatattat
     1741 gttacctaag gatttatatt tatataaggc caatgataga tacggtgagg cctatgaaaa
     1801 tctagcaaaa atactttatc ctgatatatt taattaaatc tggtggatat aatgggaaaa
     1861 aagttgacac aatcttcaaa atataatttt ttagtaattc ttattctcat aattgttttt
     1921 ataactagct tttttctaag tgttggcaat ggagcagtta aattagggcc tagggagatt
     1981 gtaaaatcta tcttcttaag acaagaaaac accaattatc aaattatatg gaatgtacgc
     2041 cttcctagaa ccttagttgc ctctttagta ggcatttgtc tgtcattatc tggagccatg
     2101 ctccaaggga taatgaaaaa ctccctagct ggtcccaata ttataggtgt atcctcaggg
     2161 gcaggcctct ttgggttgat aatattaata ctttaccccg agtattatta tctggtacca
     2221 ttaggcgcct tctttggagc tcttattgcc acactgctta tatatatatt agcttggaaa
     2281 gagggagtac agccatctcg tttaatttta gcaggggtgg cagtctcctc catctttt
//
LOCUS       DATGSI010000234         1411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_113112, whole genome shotgun sequence.
ACCESSION   DATGSI010000234 DATGSI010000000
VERSION     DATGSI010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113112"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1411)
                     /locus_tag="VK031_03595"
     CDS             complement(<1..>1411)
                     /locus_tag="VK031_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53031.1"
                     /translation="AQGSGEYTVQDSEVVIKDKFGNIIEQDSITAEGSKEFTVNTELP
                     VKNTIGQDVGTEIDGEIIVGDSNITLKDSEDNILSSFDVPAENNEEKVIDDSTAIIKD
                     SEGNILKSENILAEGSKDITITDSTSTIKDSSGNTLYSENILAEGTKETTIADSTYVI
                     KNQDGDIIAQGSILAEGSVEIVVVGYRPLILTYNTNLYTDAIIELPNVGTDPKDIIVR
                     DSLGVVVKTLVGNTDDIVMITDLPAGIYDVEVSMEETSRMIFNPGGRERVQATLVELK
                     QWGDVVWGSNLNYLFMGCENLMITADDLMKMSTVGVTDRVMTQAFERIGGITNMEWVE
                     ALPFYTMTNIFGLFHQADGVIDVVLDFNNASYLTVVGQMFRDGDFKTITINNMGSTLT
                     ATGQILYTTGVPNLEKVLFNDLQLSQNISGWRNGTAQDYITFFENIGDRSGKDQRTIT
                     IRTTEWDKLDANQKDYVDNI"
BASE COUNT          416 a          308 c          161 g          526 t
ORIGIN      
        1 aaatattatc cacataatct ttttgattag catccaattt atcccattct gttgttctaa
       61 ttgttatagt tctttggtct ttgccacttc tatctcctat attttcaaag aatgtaatat
      121 aatcttgagc agttccattt ctccaacctg aaatgttttg acttaattgt aaatcgttga
      181 ataacacttt ctcaagattt ggaacacctg tagtgtaaag aatttgacct gttgctgtta
      241 aagtgctacc catattatta attgtaatag tcttaaaatc tccatcccta aacatctgcc
      301 caacaacagt taaatatgaa gcattgttaa agtctaacac cacatctata acaccatctg
      361 cttggtgaaa taaaccaaaa atatttgtca ttgtataaaa cggtaaagct tctacccact
      421 ccatatttgt tattcctcca atcctttcaa aggcttgggt cataacccta tctgtaacac
      481 caactgttga cattttcatt aagtcatctg ctgttatcat tagattctca catcccataa
      541 ataagtagtt taagttactt ccccaaacaa catctcccca ctgttttaac tctactaaag
      601 ttgcttgaac tctttctcta ccacctgggt taaaaatcat tctgcttgtt tcttccatag
      661 atacctcaac atcataaatt cctgcaggta aatctgttat cattacgatg tcatctgtat
      721 ttcctactaa ggttttaact accacaccta aactatccct aacaattata tccttagggt
      781 ctgtccctac attaggcaat tcaattattg catctgtgta taggttggta ttatatgtta
      841 atattagtgg tctataccct accacaacaa tttccacact accctctgct aaaatagaac
      901 cctgtgctat aatatctcca tcttgatttt taataacata agtagaatct gcaatagttg
      961 tttccttagt accttctgct aatatatttt cagaatataa agtattacct gaactatctt
     1021 taatagttga tgtagaatct gtaatagtaa tatctttaga accctccgct aatatatttt
     1081 cagatttaag gatattaccc tcgctatctt taataatagc ggtactgtca tctattacct
     1141 tttcttcgtt gttttcagct ggtacatcaa aactagataa tatattatct tcactatcct
     1201 ttagagtaat attactatct cctacaataa tctcaccatc aatttcagtt cctacatctt
     1261 gtcctatagt attttttaca ggtaattctg tgttcactgt aaattcttta ctaccctccg
     1321 ctgtaatgct atcttgctct attatattac cgaacttgtc tttaataact acttcgctat
     1381 cctgaactgt atactcccct gaaccttgcg c
//
LOCUS       DATGSI010000235         4776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_113134, whole genome shotgun sequence.
ACCESSION   DATGSI010000235 DATGSI010000000
VERSION     DATGSI010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113134"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VK031_03600"
     CDS             complement(<1..321)
                     /locus_tag="VK031_03600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HLS53032.1"
                     /translation="MHRFFVNSEQIRDSTISIIGKDVNHIKNVLRLSPGDKLEVVAHN
                     IVYLCSLKDFLDEELVLKIISKAPGTNEPDVELVLYQALAKGSKMDLIIQKGTEIGVR
                     DFYGV"
     gene            complement(330..1268)
                     /gene="prmA"
                     /locus_tag="VK031_03605"
     CDS             complement(330..1268)
                     /gene="prmA"
                     /locus_tag="VK031_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HLS53033.1"
                     /translation="MKWTEVIVKTASQYEDIVSHILYEAGAQGLAIEDPNDVLELSQM
                     ESDWYFVDINLMDFPKEGILIKAYYPEERDIEAIVAFIREEIEIKPLENEGLSLGKVM
                     LNQVDDEDWAESWKRFYKPTKVGENIVIKPSWEKYKAGSNELVVELDPGMAFGTGTHE
                     TTNLCIEALEAYVKEGNLIYDIGCGSGILSIVAAKLGAGQVIGIDLDATCVKVSKENI
                     RLNKLEDKIDIKEGNLLDVVEGKADLIVANIIAEIIKDMVGDLPSYLNKGGHFISSGI
                     ILDKVDLVKEALLKEGFEIKEIKTLGEWACIIAQLN"
     gene            complement(1370..2491)
                     /gene="dnaJ"
                     /locus_tag="VK031_03610"
     CDS             complement(1370..2491)
                     /gene="dnaJ"
                     /locus_tag="VK031_03610"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HLS53034.1"
                     /translation="MRDFYEILNVDKNASDDEIRRAYRQLAKKYHPDLNPDNEEAEQK
                     FKEATAAYEVLSDPDKRARYDRFGHAGVDPQAGGQDFGGFGDIFDDIFDIFGGGFGGF
                     SQERRRRPGPTRGADLRYDLNIDFKEAVFGTEKEIQIRRTENCSTCQGEGAKPGTGKE
                     TCSTCNGTGEVRYAQQTAFGQFVRVGTCDSCKGEGEIIREKCTSCHGTGKESKTRRIK
                     VKVPAGVDNGSIISIRGEGEGGDRGGPAGDLYIYISVKEDEIFKRRGNNIYLEVPITF
                     TEAALGAEIEVPTLEGIEKFTIPAGTQTGTEFRLKNKGVPNLRSAGRGDIYFKAVVQV
                     PTKLTEKQKELLLEFSKASGEEYKKDKKGFFNKVRDAFQ"
     gene            complement(2569..4398)
                     /gene="dnaK"
                     /locus_tag="VK031_03615"
     CDS             complement(2569..4398)
                     /gene="dnaK"
                     /locus_tag="VK031_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HLS53035.1"
                     /translation="MGKIIGIDLGTTNSAVAVMEGGEPIIISNVEGNRTTPSVVAFTK
                     EDERLVGETAKRQAITNPERTIQSIKRKMGSDHTENIDGKDFSPQEISAMILQKIKAD
                     AESYLGEEVTEAVITVPAYFTDSQRQATKDAGRIAGLEVKRIINEPTAAALAYGMDKG
                     EGEHKIMVFDLGGGTFDVSILSLADGVFEVLATRGNNHLGGDDFDNVLIDYINDEFKK
                     EHGIDLKADKMSLQRLKEAAEKAKKELSTTMSTNINLPFITATQSGPLHLNMDITRAK
                     FEELTNHLVEKTMEPVRAALKDAGLAANEVEKVLLVGGSTRIPAVQEAVKRLVGREPQ
                     KDINPDECVALGAAIQGGILAGEFGGDILLLDVTPLSLGIETLGNVTTKLIERNTTIP
                     TKKSQIFTTAADNQTSVDIHVLQGERELAKDNITLGRFQLTGIAPAPRGVPQIEVTFD
                     IDANGIVNVSAKDLGTGKEQSITITASTNLTEEEIEEKVREAEKYAEEDRKKKEEIEV
                     RNNADSMVYQTEKTLKDLEGKISDDEKTQVEARLEELKKALEGDDIEDIKNKTEALTQ
                     EFYKISEKLYQNMDPDAAGGAAGAPGDDDDDIVDGDYEVVDED"
     gene            complement(4417..>4776)
                     /locus_tag="VK031_03620"
     CDS             complement(4417..>4776)
                     /locus_tag="VK031_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HLS53036.1"
                     /translation="KKRTEKEKEGLVAFGIETLACGLFPILDDFERALESQTDKVGSF
                     YEGISMIYEQLLKLLDKNEIKEIDALHQPFDPNFHNAMSMEESEEYEEGTIIQVYQKG
                     YMLKDKVIRPAAVIVAK"
BASE COUNT         1265 a         1151 c          645 g         1715 t
ORIGIN      
        1 aactccataa aagtctctaa cccctatttc agtacccttc tgaattatta aatccatttt
       61 acttccctta gccagggcct gatataaaac aagttccaca tcaggttcat tggtgccggg
      121 agcctttgaa ataatcttaa gtacaagctc ctcatctaga aaatctttta aactacacaa
      181 atatactata ttatgagcca ccacctccag cttatcacct ggagaaagcc tcaaaacatt
      241 tttaatatga ttaacatctt tacctataat tgaaattgtg ctatctctaa tttgttctga
      301 atttacaaaa aatctatgca ttcaatcacc taatttaact gggctattat acaggcccat
      361 tcacctaaag tttttatttc cttgatttca aacccttcct ttaaaagggc ttccttaacc
      421 aaatctacct tatctaaaat tattccagaa gaaataaaat ggccaccttt atttaagtag
      481 cttggaaggt ctcctaccat atcctttata atctcagcta taatattggc tactataaga
      541 tctgcctttc cttcaaccac atccaatagg ttaccttctt taatatctat tttgtcttcc
      601 agcttattaa gccttatatt ttccttagag acctttacac aggtagcatc caggtcaatg
      661 cctattacct gtcctgctcc taatttggca gctacaatac ttaaaatccc actaccacag
      721 cctatatcat atattaggtt gccttccttt acataagctt ctagggcctc aatacacaag
      781 ttagtagttt catgagtacc agtaccgaag gccatacctg gatccaattc cactacaagc
      841 tcattagatc ctgccttata tttttcccag ctaggcttta ttacaatatt ttctcctacc
      901 tttgtaggct tataaaatct tttccaagac tctgcccaat cctcatcatc tacctggttt
      961 aacatgactt ttcccaagga tagaccctca ttttctaagg gtttgatctc aatttcttcc
     1021 ctgataaaag ctacaatagc ctcaatatcc ctctcctcag gataataggc cttgattaat
     1081 ataccctctt tagggaaatc cattaagtta atatccacaa agtaccagtc gctttccatt
     1141 tgagataatt ctaaaacgtc attaggatcc tctatggcca gcccctgggc cccagcctcg
     1201 tacaaaatat gagagactat atcttcatat tgggaggcag tctttactat tacctcagtc
     1261 catttcatcg gcttacacat cctttaattg gtcttttata attataacct aacctttatt
     1321 tgaataccag tgtagtcttt aaaaaaacaa tcaccacagg atgattgttt tactggaagg
     1381 catctcttac tttgttaaaa aaacccttct tatctttttt atattcttcc ccagaggcct
     1441 tggaaaattc taataacaac tccttctgtt tttctgttag cttggttggt acctgtacaa
     1501 ctgccttaaa atatatgtct cccctacctg cagatcttaa attaggcact cccttgtttt
     1561 ttaacctaaa ttctgttcct gtttgtgttc ctgccggtat ggtaaacttc tctatgcctt
     1621 ctaaagttgg tacctctatt tctgcaccta aggctgcctc agtaaaagta atgggcacct
     1681 ctaaataaat attatttcct cttcttttaa atatttcatc ttcttttaca ctaatataaa
     1741 tatataggtc tccggccgga ccacctctgt ccccaccttc tccttcacct ctaatggata
     1801 ttatggatcc attgtcaacc ccagcaggga cctttacctt aatccttcta gttttacttt
     1861 ccttgccagt tccgtgacaa ctggtacatt tttctctaat tatttctcct tcacccttac
     1921 aactgtcaca ggtacctacc cttacaaact gtccaaaggc agtttgctgg gcatatctta
     1981 cctctcctgt accattacaa gtggagcagg tttctttacc tgtgcctggt ttagctcctt
     2041 ctccctggca agtagaacag ttttcagttc gcctaatttg tatctccttt tcagtaccaa
     2101 atactgcctc tttaaagtct atatttaaat cataccttaa atcagcacct ctagtaggcc
     2161 caggccttct ccttctttcc tgggaaaaac caccaaatcc cccaccgaaa atatcaaata
     2221 tatcgtcaaa tatatctcca aaaccaccaa aatcctgacc accagcctga ggatccaccc
     2281 ctgcatgccc aaacctgtcg tatctagccc ttttatcagg gtcacttaaa acttcatagg
     2341 ctgcagtggc ttccttaaat ttttgttctg cctcttcatt gtcaggattt aagtcaggat
     2401 gatatttctt tgccaattgc ctatatgccc ttcttatttc atcatctgag gcatttttat
     2461 ctacattcaa gatttcatag aaatccctca atccatctca ccaactttca aataaataga
     2521 atacaaaatt caaagctaaa tcttaagatt tagctttgaa tttaattact aatcttcatc
     2581 tactacttca taatctccat caactatatc gtcgtcatca tcgcctggag caccagctgc
     2641 tcctcctgct gcatctggat ccatattttg atatagtttt tcagagatct tgtaaaactc
     2701 ctgagttaaa gcctcagtct tgtttttaat atcttctata tcatcgccct ctaaggcctt
     2761 tttcaattcc tctagtctag cttcaacctg agttttctca tcatcagaaa ttttgccttc
     2821 caggtctttt aaagttttct cagtttgata aaccatagag tctgcattgt ttctaacttc
     2881 aatttcttct tttttcttcc tgtcttcttc tgcgtatttt tcagcttccc ttactttctc
     2941 ttcaatctct tcttcagtta aattagtaga tgcagtaata gttatgcttt gctccttgcc
     3001 tgtacctaaa tctttggcac ttacatttac tataccatta gcatctatat caaaggttac
     3061 ctcaatttgt ggtactcctc ttggagctgg tgctatacct gttaattgga acctacctag
     3121 agtaatattg tctttggcca gctccctttc accttgtagt acatgtatat caacagatgt
     3181 ttgattgtct gctgcagtag taaatatctg tgatttctta gttggtattg tagtgtttct
     3241 ttcaattagc tttgtagtta cgttacctaa ggtttcaata cctagtgaaa gtggagttac
     3301 atccagtagt aaaatatctc caccgaattc tccagccaag ataccacctt gaattgctgc
     3361 tcctaaggcc acacattcgt ctgggttaat atccttttgt ggctccctgc ctaccagcct
     3421 ttttactgct tcttgtactg ctggtattct agtagaacct cccaccagta ggactttttc
     3481 cacctcattg gctgccaaac ctgcgtcttt taaggctgct cttacaggtt ccattgtttt
     3541 ttcaaccaga tggttggtta attcttcaaa ctttgccctg gtaatatcca tatttaaatg
     3601 aagtggtcca gactgggttg cagtaataaa tggtaaattt atatttgtac tcatggtagt
     3661 agataactct tttttggcct tttcagcagc ctcttttaat ctttgtaaac tcatcttgtc
     3721 tgccttaagg tctataccat gttccttttt aaattcatca tttatataat cgattaaaac
     3781 attgtcaaag tcatctccac ctaagtggtt atttcccctt gtggccaaaa cttcaaatac
     3841 tccatcagct aaggatagga tggaaacgtc aaaggtacct ccacctaggt caaacaccat
     3901 aatcttgtgc tctccttctc ccttatccat accataggct aaggctgctg cagtaggctc
     3961 atttataatt ctttttacct ctaaacctgc tattctacca gcatcctttg tagcctgtct
     4021 ctggctatct gtaaaatatg caggtactgt aattacagct tccgttactt cctcccctag
     4081 gtaactttca gcatctgcct taattttttg tagaatcata gcagatattt cttgaggaga
     4141 gaaatctttc ccatctatat tttctgtatg gtcactaccc atttttcttt taatagactg
     4201 tatagtcctt tcagggtttg ttattgcctg tcttttggct gtttcaccta ccaaacgttc
     4261 atcttcttta gtaaaggcaa caacagatgg ggtagttcta ttaccttcca catttgatat
     4321 aattattggt tctccacctt ccattacagc tacggcagag ttagtagttc ctaaatcaat
     4381 tcctattatt tttcccatat caagtcctcc ttaaacttat ttagctacta taactgctgc
     4441 aggccttata accttatcct ttaacatata tcctttttga taaacctgaa ttatagtacc
     4501 ttcttcatat tcctctgatt cctccataga catagcatta tggaagttag gatcaaaggg
     4561 ttgatgcaaa gcatctattt ctttaatttc atttttatct agtaatttta gcaactgttc
     4621 atatatcatg ctaatacctt cataaaagct tcctacttta tcagtttggc tctctaaagc
     4681 cctttcaaaa tcatctagaa taggaaatag cccacaagca agagtttcaa taccgaaagc
     4741 aactaatcct tccttttcct tttcggttct tttctt
//
LOCUS       DATGSI010000236         1520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_489873, whole genome shotgun sequence.
ACCESSION   DATGSI010000236 DATGSI010000000
VERSION     DATGSI010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489873"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VK031_03625"
     CDS             <1..469
                     /locus_tag="VK031_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HLS53037.1"
                     /translation="QARWDIPYYNFTKKELLEAARKDYLDRTPQILEFSRHFKLGYDI
                     AEDELDSFLVDIVNMINNKYSINDIIKMLEEHFEIVDFEQMHTISILVMEINNNTKMW
                     ILKGNSPNDLFGSQDLSSAGSGSTIVRTRRKIGRNEPCPCGSGKKYKRCCGRN"
     gene            complement(625..939)
                     /locus_tag="VK031_03630"
     CDS             complement(625..939)
                     /locus_tag="VK031_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLS53038.1"
                     /translation="MDKKSLRTIRLSKNLLQVDLAHRAGISPSYYSLIESGHRNPSLK
                     TASRLCRCLGISLDVFYTALQTIQKNDNSEEIYQLKGSSSCIYSICNSNGLCRRNQTR
                     QI"
     gene            1129..>1520
                     /locus_tag="VK031_03635"
     CDS             1129..>1520
                     /locus_tag="VK031_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HLS53039.1"
                     /translation="MPMDNNMLKDENFTPVNFELETTSIWSFPQRGNWATHNGSYRGN
                     WSPYVPRNIILRYSNRGDLVLDQFVGSGTTMIETFLLGRKGIGVDINKNSLKIAKRNI
                     LKSRYGKGNIPRLHLGDARRLNFIKDGS"
BASE COUNT          528 a          211 c          292 g          489 t
ORIGIN      
        1 acaggctaga tgggatatac cctattataa ctttacgaag aaggagctat tagaagccgc
       61 aagaaaagat tatctggata gaacaccaca aattttagag ttttcccgac attttaagtt
      121 aggctatgat atagctgagg atgagcttga ttctttttta gtagatatag ttaatatgat
      181 taataataag tattccatca atgacataat aaaaatgtta gaggaacact ttgaaatagt
      241 ggattttgaa caaatgcata caatttccat attggtaatg gaaattaaca ataatacaaa
      301 aatgtggatc ttaaagggta actcccctaa tgacttattt ggtagtcagg atttgtcatc
      361 ggcaggtagt gggtctacaa ttgtgagaac tagaagaaaa attggtagaa atgaaccttg
      421 cccttgtggt agtgggaaaa agtataaaag atgttgtgga agaaactaga gactttgcat
      481 catataaaaa tatttgaatt catataaacc atggccataa aatattttgg gttcatggtt
      541 tatatttgtt ttaagctgat atacaaagag atccgtctta gtttaatttg aaaaccagct
      601 taggaaaaat taatacaagt atggttaaat ttgtctagtt tgattcctcc tgcataatcc
      661 attcgaatta catatgctat agatgcaaga acttgaacct ttcaactgat aaatctcttc
      721 actattatca tttttttgaa tcgtctgtag tgcagtataa aacacatcta ggctaattcc
      781 caaacatctg caaagcctgc tggctgtttt taatgatgga tttctatgac ctgattcaat
      841 taaagaataa taggatggac tgataccagc cctatgggcc aagtcaactt gtaaaaggtt
      901 tttgctaagt ctaatagttc ttaaactttt cttgtccatt tacatcccct cttttaccct
      961 tttagttaag atatagattt aaggaaaaaa atttactagg gaatagattt taaaagttta
     1021 aataagatgt aaatgtctct tggaagtgtt attttcatgt tgcttgacaa ctgaacataa
     1081 gtttcataaa ataatattgt gtaacatatt ttcatgaggt gattttcgat gcctatggat
     1141 aataatatgt taaaggatga gaattttact ccagtaaact ttgaattgga gactaccagt
     1201 atatggagct tccctcagag gggaaattgg gctacccata atggaagcta cagggggaat
     1261 tggtcacctt atgtaccgag aaatattatc ttaagatact ccaatagggg agatctagta
     1321 ttagatcaat tcgttggtag cggaaccacc atgattgaaa ccttcctttt aggaaggaaa
     1381 ggtatagggg tggacattaa taaaaattcc ctaaaaatag ctaagagaaa catattaaag
     1441 agtaggtatg gaaagggcaa tattcctaga ttacatctag gggatgcaag gcgactgaat
     1501 tttattaaag atggtagtat
//
LOCUS       DATGSI010000237         1260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_207460, whole genome shotgun sequence.
ACCESSION   DATGSI010000237 DATGSI010000000
VERSION     DATGSI010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207460"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VK031_03640"
     CDS             <1..556
                     /locus_tag="VK031_03640"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HLS53040.1"
                     /translation="GITLGTLTANIISSDTELILNSIVGVGIWGGLVLIITWLSKKSS
                     ILKVIFQGKPIIIIKQGKLLAESLRTAKLNIDDLLMMLRNKNIFSVRDVEYAILEPNG
                     ELTLLERPELLPVTRSDMRLDVNDYKNLPASIIIKGKMIEKNLEELGLDPAWIYQELE
                     RQEVKNIEEVFYAEITEEGKLYIN"
     gene            complement(702..1151)
                     /gene="msrB"
                     /locus_tag="VK031_03645"
     CDS             complement(702..1151)
                     /gene="msrB"
                     /locus_tag="VK031_03645"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HLS53041.1"
                     /translation="MRKKYKKPSQEEIKRKLTDIEYRVTQKDATERPFSHEYDDHFEE
                     GIYVDIVSGEPLFSSKDKYDAGCGWPSFTKPLEKEHIINKKDTSHGMIRTEVRSKHAD
                     SHLGHVFTDGPQDKGGLRYCINGAALKFIRKEDLEEEGYGEYLSLFE"
BASE COUNT          424 a          217 c          215 g          404 t
ORIGIN      
        1 tggaattact ttaggcaccc taactgccaa tataattagc tctgatacag aattaatatt
       61 aaatagtata gttggagtag gtatttgggg aggcctagtt ttaattatta cctggttatc
      121 caagaaatct tccatcttaa aagttatctt tcagggcaaa ccaatcataa ttattaagca
      181 gggaaaattg ttggctgaat ctcttaggac tgccaagctt aatatagacg acttgctgat
      241 gatgctgcgg aataaaaata ttttttcagt aagagatgtg gaatatgcaa tcctagagcc
      301 caatggtgaa cttaccttat tagaaagacc ggagctttta ccagttacaa gaagtgatat
      361 gaggctagat gtaaatgatt ataaaaatct acctgcttcc atcataatta aaggtaaaat
      421 gatagagaaa aacctagaag aattaggtct agatccggct tggatttatc aggagttaga
      481 gaggcaagaa gttaaaaata ttgaagaggt tttctatgcg gaaataacag aggagggaaa
      541 gttatatata aattaagctg aaaagcttag caaataatac taccccctaa gcctgaatat
      601 agagttaggg ggtaatatat ttgtaggcct ttaaattata ggtagttggg aatatttaaa
      661 ggcctataaa ataactggac taaaatcttg ggaatggctc cttattcaaa caaacttaaa
      721 tattccccat aaccttcttc ttccaaatcc tctttcctga taaatttaag ggcagcacca
      781 ttgatacaat atcttaagcc acccttatcc tgaggtccat cagtaaagac atggcccagg
      841 tgagaatctg catgcttact tcttacttca gttctaatca taccatggga agtatccttc
      901 ttatttatta tatgttcttt ttctaaaggc ttagtaaagc taggccagcc acaacctgca
      961 tcatatttat ccttggaact aaacaagggc tcaccactta ctatatccac atagatacct
     1021 tcttcaaagt gatcatcata ctcatgagaa aagggtcttt cggtagcatc tttttgagtt
     1081 accctatatt ctatatcagt tagcttcctt tttatttctt cttgactagg ttttttatac
     1141 ttcttcctca tttaatcagc ctcctagaaa tatattaccc atagtaaact agatatataa
     1201 taagaatata taaacttgtt atagttttaa aagacaaagt ccatagccat aaattatggt
//
LOCUS       DATGSI010000238         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_585303, whole genome shotgun sequence.
ACCESSION   DATGSI010000238 DATGSI010000000
VERSION     DATGSI010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585303"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VK031_03650"
     CDS             <1..246
                     /locus_tag="VK031_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53042.1"
                     /translation="NLDIGHAKLGKVPLEEWISQLNDYISYIHIHSNDGLYDRHLELT
                     DKEIERLYLLLDKYKLNPILSLEYKIDNLTKEIKRYK"
     gene            311..1312
                     /locus_tag="VK031_03655"
     CDS             311..1312
                     /locus_tag="VK031_03655"
                     /inference="COORDINATES: protein motif:HMM:NF025393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="questin oxidase family protein"
                     /protein_id="HLS53043.1"
                     /translation="MSIGKIINHYGRIQSPYARGLVNHLPMAQVALYKMDGDLKKIKN
                     YSQIYIKESKISPVKTKYEKFINFKEHLGFRERYESCLELINHRLEEEDIHNLIGELL
                     NTYELGMSSGIFHTIIRLAYAVEGYSMDNILSEEIARALAYYITAYREAKILKRPIKW
                     KQIQEEMIKLSKDKEMEEILNQAETLGQGLKGLYNSQAYLEKGFVLEGSENEKIKGLL
                     SLVIPTYYKSGNIVVLHSITGLHALIVLKDYFKDFNKALDILTSSIITHLLAVGSLDY
                     QAEINPDTELSWDCLKAKASESTDVHALKLTYTAYELDKKYDIPGLKDIALKRIKNK"
     gene            1416..2189
                     /gene="pgeF"
                     /locus_tag="VK031_03660"
     CDS             1416..2189
                     /gene="pgeF"
                     /locus_tag="VK031_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HLS53044.1"
                     /translation="MAYKIVRYNEKTYITVPKLTKLGLKHCFTSKDMDLGTKTNPSTK
                     RIKENLKDIYNFLNISPEGLFSGIQTHSKNIVTVKDTESGNQDPFGRSFPNTDGLITD
                     KKNIALITRYADCIPILIYDPKRQVQANIHSGWKGTWQEIVVNGINIMREEYESQPED
                     LIVVMGPSIGKDDFEVDLDVMEKFKKKFDFHSEVIARKNNKKYLIDLQGINRRRLLES
                     GVKEENIIAIDISTYQSPLLHSYRRDGKDFGLMGAISCM"
     gene            complement(2221..>2542)
                     /locus_tag="VK031_03665"
     CDS             complement(2221..>2542)
                     /locus_tag="VK031_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLS53045.1"
                     /translation="PISNAPLAMVILADAEITDTWIEDAAIMATIVQVTAHSLGLGSC
                     WVQVRERFTTDGQSVDSFVKDLLNIPDKYQVSAIISIGYPAEDKDAYTKDKLDYNKVH
                     HNKF"
BASE COUNT          939 a          391 c          480 g          732 t
ORIGIN      
        1 aatctagata ttggacatgc aaagcttggg aaagttcctt tagaagaatg gatttcccaa
       61 ttaaatgatt atatttctta tatccatatt catagtaatg atggcctata tgataggcat
      121 cttgagctaa cagacaagga aattgaacgc ctttacttgt tattggataa atataaatta
      181 aatcctatcc tgtccttaga gtataaaatt gataatttga ccaaggaaat taaaagatac
      241 aaatagttat tagtagtttt taaagggtat aagtaaataa gaagtcaaat agtttaggaa
      301 gaggtatatt atgtctatcg gaaaaattat taatcactat ggcaggattc aatccccata
      361 tgcaagaggg ttagtgaacc atttacctat ggcacaagtg gctttataca agatggatgg
      421 agatttaaaa aagataaaaa actactccca gatttatatt aaggaaagca aaataagtcc
      481 agtgaaaact aaatatgaaa aatttataaa ctttaaagaa catctaggat ttagagaacg
      541 gtacgaatca tgtttagaac ttataaacca taggttagaa gaagaagata ttcataatct
      601 aattggggag ctattaaaca cttatgaact aggtatgtct tcaggtattt tccataccat
      661 aattagattg gcctatgctg tggaaggata ttcaatggac aatatcctat ctgaagaaat
      721 agctagagct ttggcctatt atattacagc ctacagagag gctaagattt taaaaaggcc
      781 tatcaagtgg aagcagattc aggaagagat gatcaaatta tccaaggata aagagatgga
      841 agagatacta aatcaagcgg aaaccctagg ccagggactt aaggggcttt ataatagtca
      901 ggcctatcta gaaaaaggtt ttgtcctgga aggtagtgaa aatgaaaaga taaaaggact
      961 tctaagcctt gtaattccta cctattataa gtcaggcaat attgtggttt tacattctat
     1021 aacaggccta catgccctaa tagtactgaa agattacttt aaagatttta ataaggccct
     1081 agatatatta accagcagta taataaccca tttgttggct gtaggttcct tagattatca
     1141 ggcagaaata aacccggata cagaactatc ctgggactgt ttaaaggcca aggcatcaga
     1201 atctactgac gtccacgccc ttaaattaac ctacactgcc tatgaactag ataaaaagta
     1261 tgacatacct ggtttgaagg atattgcttt aaagaggatt aagaacaaat agaagaactc
     1321 cctacttaac tagggatttc ttgtattttt aaattaatat tggcaattct taaatcttat
     1381 ccatattatt atataaagaa taaaaggagc agattatggc atataaaata gtaaggtata
     1441 atgaaaaaac ttatataaca gttcccaaat taaccaagct aggcttaaaa cattgtttta
     1501 ccagcaaaga tatggattta ggtacaaaga ctaacccttc aacaaagaga atcaaagaaa
     1561 atttaaagga tatttacaac tttttaaata taagccctga aggattattt tcaggtattc
     1621 agacccattc gaaaaatata gtcacagtta aagacacaga atcaggaaac caggatcctt
     1681 ttggaagaag ttttcctaat acagatggct taattacaga taagaaaaat atagccttaa
     1741 taaccagata tgctgattgc atacctattt taatctatga tcctaaaaga caggtccagg
     1801 ccaacataca ttctggctgg aagggaacct ggcaggagat agttgtaaat gggattaata
     1861 ttatgaggga agaatatgaa tcccagcctg aagatttaat agtagtaatg ggtccttcca
     1921 ttggcaagga cgattttgag gtagacctgg atgtgatgga gaagtttaag aaaaaatttg
     1981 actttcactc tgaagttata gcaagaaaga ataataaaaa atatctaata gatctacagg
     2041 gaataaacag aagaaggcta ctggaatcag gagtcaagga ggaaaatata atagcaatag
     2101 acatatcaac ctatcaaagt cccctgcttc attcatatag gagggatgga aaggactttg
     2161 gcctaatggg tgccatcagc tgtatgtaaa acttcctccc taggctttgg gaggaagctg
     2221 ttaaaattta ttatgatgaa ccttgttata atctaattta tccttggtgt aggcatcttt
     2281 atcttctgct ggatagccta tagatataat ggctgatacc tgatatttat caggaatatt
     2341 taataaatct tttacaaagg aatctacact ttggccatca gttgtaaatc tttctctcac
     2401 ctgcacccag catgaaccaa ggcctaagga atgggcagta acttgaacaa tagttgccat
     2461 tattgcagca tcctctatcc aagtgtcggt aatttcagca tcagccagaa taaccatggc
     2521 caagggagca ttagatatag gt
//
LOCUS       DATGSI010000239         4913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_537472, whole genome shotgun sequence.
ACCESSION   DATGSI010000239 DATGSI010000000
VERSION     DATGSI010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537472"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /gene="ruvB"
                     /locus_tag="VK031_03670"
     CDS             complement(<1..883)
                     /gene="ruvB"
                     /locus_tag="VK031_03670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HLS53046.1"
                     /translation="MGDNKNRIISRNLKEEDLDSEVTLRPKWLTEYIGQDKAKEKLNI
                     FIQAAQGRKEALDHVLIYGPPGLGKTTLANIIANEMGVNIRITSGPAIERPGDLASIL
                     TNLSDDDVLFIDEIHRINRTVEEILYPAMEDFALDIIIGKGPSARSVRLDLSRFTLIG
                     ATTRAGLLTSPLRDRFGVMLNLDLYDTDSLKKIIQRSARILNIEIDDKGAMEIAKRSR
                     GTPRIANRLLKRVRDYAQVMEDGVISYEVAKKGLEILEVDPLGLDAIDRKLILALIEN
                     FNGGPVGIDTLSATTGEE"
     gene            complement(898..1482)
                     /gene="ruvA"
                     /locus_tag="VK031_03675"
     CDS             complement(898..1482)
                     /gene="ruvA"
                     /locus_tag="VK031_03675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HLS53047.1"
                     /translation="MINFIIGNVVRIEDEYVVIQNNGIGYKVFTSANSKMDLEIGKEN
                     QMLHTQMFVRDDGIYLYGFTSLEEMDMFNLLLRVSKVGPKIGIGVLSTLSPRQIQKAI
                     LTGDVPTLTKAPGIGKKTAERIIVELKDRIDIPLIETEEVEVKSLEYNQAIDGLLSLG
                     YSRFEVEKAIKSLDYRKMTVEDIIREALKKLSSK"
     gene            complement(1500..1994)
                     /gene="ruvC"
                     /locus_tag="VK031_03680"
     CDS             complement(1500..1994)
                     /gene="ruvC"
                     /locus_tag="VK031_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HLS53048.1"
                     /translation="MIILGIDPGIAIVGYGLIECRGNSFKAIEYGTIVTDSKLLFPDR
                     LKIVYDKLGEIIDEYKPNDMAIEELFFNKNIKTAIKVGQARGVEILAGVNKGLDIYEY
                     TPLQIKQAIVGYGRAEKRQVQEMVKLLLNLKEIPKPDDAADALAVAICHGSSLKFKED
                     FRMK"
     gene            complement(2072..2644)
                     /locus_tag="VK031_03685"
     CDS             complement(2072..2644)
                     /locus_tag="VK031_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53049.1"
                     /translation="MKNRRFITVFLFSILLFTVSFSLGYRLMEGRFGKDKLETDPNDQ
                     GLKIVKEENRIGPNTLIEERTHYKECGDLIENTNKVKEKLVNMDKGEYETYLRKNHPD
                     FRLISFSNHKIIIWREKDHLCKNHFIIGEEGGYIAIFIVDDNGNRVVNNVFEEYPISL
                     LVEIDQEKIKNGIVVDSKEELYDILENFIT"
     gene            complement(2707..3441)
                     /locus_tag="VK031_03690"
     CDS             complement(2707..3441)
                     /locus_tag="VK031_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53050.1"
                     /translation="MALSNSKAHNLYEKLKYNFLFNNDINCIHILLNLYELENNINNI
                     FPKYVSLNYLRRQIRRLLKDRRGNHLIAYNLGQLIHEDINRLELFMYLEGYKQGYLNN
                     RIANSLESKVIKHFSISELYNMKYLVHFDSSIVEINKLKAKIFADLRKEEKKNKQLEN
                     IISNYTSNVIKSKVLSLNKYLDKQLAIDYDSQSPNIREDEALLTLDELKNIYKEVEKI
                     ILRNGKKLYYDAYWNGLNDRVLERYK"
     gene            complement(3746..4381)
                     /locus_tag="VK031_03695"
     CDS             complement(3746..4381)
                     /locus_tag="VK031_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HLS53051.1"
                     /translation="MFADTHKIIANQIYNDIYRIYDVKLNKRKLIQGSLIPDIFPKYR
                     LIRHYKKESIDYIVDEIIKVIYIGRYLEFNKVLDPIAINLLSKKIGIISHYLSDFVCL
                     PHARRWTFFNSMVKHIKYETALDSFAKGHSFKRNVIDSSDVEIIDRDISKLNGQIKDY
                     IESVVDEYSLKESFENDLNFALELSLKITYFILDTIKIYGKELHKELIFEI"
     gene            4742..4818
                     /locus_tag="VK031_03700"
     tRNA            4742..4818
                     /locus_tag="VK031_03700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4776..4778,aa:Arg,seq:acg)
BASE COUNT         1482 a          954 c          617 g         1860 t
ORIGIN      
        1 tttcctcacc tgtagttgca gacaaggtat ctatacctac aggcccacca ttgaagtttt
       61 caatcagagc caggattaat tttctatcta tggcatctaa ccctaaagga tctacttcaa
      121 ggatctccag ccccttctta gccacctcat aacttattac tccatcttcc attacctggg
      181 catagtctcg cacccttttt aataacctat ttgctatcct tggtgttcct ctggagcgtt
      241 ttgctatttc catagccccc ttatcgtcaa tttctatatt taggatcctg gcagaccttt
      301 gaatgatttt ttttagacta tcagtatcat acagatctaa gtttaacatt acaccaaatc
      361 tatcccttaa aggtgatgta agtaatccag ccctggtggt tgctccaatc agggtaaatc
      421 ttgataagtc taaccttact gagcgggcag aaggcccttt gccaattata atatctaggg
      481 caaagtcctc catggccgga taaagaattt cctccacagt cctatttatt ctatgaattt
      541 catctataaa taaaacatca tcatcactaa ggttggttaa tatactggct aagtcacctg
      601 gcctttctat ggccggccct gatgttattc taatattaac ccccatttca ttggctataa
      661 tatttgctaa ggttgtttta cctaagcctg gcggtccata tattaacaca tgatccaagg
      721 cttcttttct accttgggca gcttgaataa agatatttag cttttcctta gctttatcct
      781 gtcctatata ctcagttagc catttaggtc ttaatgtcac ttcactgtcc aggtcttctt
      841 ctttaagatt tctagatata attctgtttt tattgtctcc catttatatt gaccccttta
      901 tttacttgac aattttttta aggcttccct tattatatct tccacagtca tttttctgta
      961 gtctaaggat tttattgcct tttcaacttc aaatcttgaa tatcctaatg ataataagcc
     1021 atctatggcc tgattatatt ctaagctttt aacttctact tcttcagtct caataagcgg
     1081 tatatcaatt ctatctttca actcaacaat tattctttcc gcagttttct ttcctattcc
     1141 tggagccttt gttaaagttg gaacatctcc agttagtatg gctttttgta tttgtcttgg
     1201 tgatagggtg gataagactc ctataccaat tttaggccct acttttgata ctcttaacaa
     1261 gagattaaac atatccattt cttccaagct ggtaaagcca tataagtaaa taccatcatc
     1321 cctaacaaac atttgagtat gtaacatctg gttttcttta cctatttcca aatccatttt
     1381 tgaattggca gatgtaaaaa ccttatatcc aattccgttg ttttgtatta ccacatattc
     1441 atcctcaatc cttacaacat tgccaattat aaaatttatc atttaaccac tcctggattt
     1501 tatttcattc taaaatcttc cttgaatttt aaagaactac catggcaaat ggcaacagcc
     1561 aaagcatctg ctgcatcatc tggcttaggt atttccttta aatttaataa gagctttacc
     1621 atttcctgaa cctgacgttt ctctgcccta ccatatccta ctattgcctg tttaatttgt
     1681 aggggagtat attcgtaaat gtctaaccct ttatttaccc cagctagaat ttctacccct
     1741 cttgcttgtc caacttttat ggcagtcttt atattcttat taaaaaaaag ttcctctatg
     1801 gccatatcat taggtttata ctcatcaata atttcaccta acttgtcgta gacaattttt
     1861 agcctgtcgg gaaacagtaa ttttgaatcg gttacaatgg taccatactc aatagcctta
     1921 aaactattac ctctacattc aattagtcca taccctacta tggctattcc agggtctatt
     1981 cctaaaatga tcataattgt ccccctaaaa agattatata atagattgta tcatacttcc
     2041 ataaaaaaac taagttagca aaccaactta gttaagttat aaaattctcc aatatatcgt
     2101 atagttcttc tttactatct actactatac catttttaat tttttcctga tctatctcta
     2161 ccaacaaact gataggatac tcctcgaata cattatttac aaccctatta ccattatcat
     2221 ctactataaa tatggctata tatcctcctt cttctcctat aataaaatga tttttacata
     2281 ggtggtcttt ttctctccaa attataattt tgtgattgga aaaggatatt aacctaaaat
     2341 caggatggtt ttttctaagg taagtttcat attccccttt gtccatatta accagctttt
     2401 cttttacctt atttgtattt tctattagat caccgcattc cttatagtga gtcctttctt
     2461 ctattaaggt attgggccct atcctattct cctctttcac aatttttaag ccctggtcat
     2521 tagggtcggt ttctagttta tcttttccaa aacgtccctc cataagccta tatccaaggg
     2581 agaagcttac tgtaaataaa agaatggaaa aaagaaaaac tgtaataaat ctacgatttt
     2641 tcattaatat ccctccatat tgtaatctag aaggatatta ttaccatatt tgaaaaatat
     2701 attcagttat ttatatcttt ctaaaactct atcatttaaa ccattccaat aggcatcata
     2761 ataaagcttt ttcccatttc taaggattat tttttccact tccttatata tattctttag
     2821 ctcgtctaat gttaggagag cttcatcctc tcttatgtta ggggattggg aatcgtaatc
     2881 tatggctaat tgcttatcta agtatttgtt taaactaagc accttagatt taataacatt
     2941 agaggtgtag ttactaatta tattttccaa ttgtttgttt ttcttttctt cctttcttaa
     3001 gtcagcaaat atttttgctt ttaacttatt aatctcaact atagatgagt cgaaatgtac
     3061 caaatatttc atattatata attccgatat ggaaaaatgt tttattacct tactttccaa
     3121 actatttgct atcctattat tcaaatatcc ctgcttgtac ccttccaagt acataaatag
     3181 ctctagtcga tttatatctt catgtataag ctgtcctaaa ttataggcaa ttaaatggtt
     3241 gccccttcta tcctttagca accttctaat ttgtcttctt aaataattta gtgacacata
     3301 tttaggaaat atattgttta tattgttttc taactcataa aggtttagga gaatatgtat
     3361 acaattaatg tcattgttaa atagaaaatt atactttaat ttttcatata gattatgtgc
     3421 tttactattt gacaatgcca tattatcccc tcctccctta caattcttac caggtcaatc
     3481 cttttttaaa cttatttata ttaaactacc aaaaaataaa gatatgtaac tttctgcaaa
     3541 tatggtttaa acttttttaa ttaacaatca atcttataaa aaaagtgaca gcatgtccta
     3601 ttatactaca aatattccta cagttgtagc ctaaatatat tatttcccaa atctttctta
     3661 agttaatttt aagttccatg gagaagaaaa aagtatatct aaagatatac ttttttaact
     3721 ttccatttat taaaataagg aaatattata tttcaaaaat cagttctttg tgaagttctt
     3781 taccataaat ctttatagta tctaatataa agtaagttat ctttaaactt aattccaggg
     3841 caaaatttaa atcattttca aaactttctt ttagtgaata ctcatccaca accgactcaa
     3901 tatagtcctt tatttgccca tttaacttac ttatatctct gtctattatc tctacatcac
     3961 tgctatctat aacatttctc ttaaatgagt ggcccttggc aaaactatct aaggccgtct
     4021 catattttat atgtttaacc atggagttga agaaggtcca ccttctagca tgtggtaggc
     4081 aaacaaaatc agataagtaa tgcgagatta tacctatttt tttactgagc aagttaatag
     4141 caatagggtc aagaacctta ttaaactcta aatatcttcc aatgtatata acctttataa
     4201 tttcatccac aatataatct atactttcct ttttataatg tctaatcaat ctatattttg
     4261 gaaatatatc tggaatcagt gaaccttgga tcaatttcct tttgtttagt tttacatcat
     4321 agatcctgta tatatcatta tagatctggt ttgctatgat tttatgagtg tctgcaaaaa
     4381 tttaaaacat ctcctcttaa aaaattaatg agcctccttg atattataat aaaaagctaa
     4441 attagtcaac aaatttagta aattctataa ctcttttatc ttatccaagg ctatacctac
     4501 agccttccta actagatagc cataagctat tgtagaactg gcaggagaaa ataagtaagg
     4561 attgcacacc cactcctcta gcgtctaata gagcattgtt ggcagcatgg gcttttgtat
     4621 aataattatg tctccaactt aaattacata tgtaaatagg caatgattag agaactttaa
     4681 tggatgcagc ttgatttttc cttgtatatt gtatataata taatggtcga tcatatatta
     4741 tgtaccctta gctcagctgg atagagtgtc tgactacgaa tcagaaggtc acaggttcga
     4801 atcctgtagg gtacgccatc tataacaggc tatattactt aggtatagcc tgtttgttta
     4861 tttttgtaaa aacaaatttg ttagttactt gttagttcta aaaattacca gcc
//
LOCUS       DATGSI010000240         1698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_207592, whole genome shotgun sequence.
ACCESSION   DATGSI010000240 DATGSI010000000
VERSION     DATGSI010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207592"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="VK031_03705"
     CDS             <1..161
                     /locus_tag="VK031_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HLS53052.1"
                     /translation="EFIYQNKFETLEELELQLAEYIYWYNHIRIHGSLGYISPVEYRY
                     RENLKLVA"
     gene            complement(471..>1698)
                     /locus_tag="VK031_03710"
     CDS             complement(471..>1698)
                     /locus_tag="VK031_03710"
                     /inference="COORDINATES: protein motif:HMM:NF019192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HLS53053.1"
                     /translation="KDAIKELVIISELNELMDLEFNPDNYTKQSWQNYIDSLDNAKKV
                     IKDGTQEEVNEATEILRNAIESLVIYEDADIRDVIEEMKLIREEDYTEKSYRELQDAI
                     EKAIKDLELKNPEINRSNIQEMLNKRNGLVNVRSLKEMIHKAEKYDSELYTRDSYNKL
                     EQSLLEANDNIINGSKESVEKALENLETAINGLVQNADELEDYRNTIVLKNPEIYTEE
                     TYKAYLEAYEAIMELEIGNTPKQEFQKLKEEFEIARDSLIIKRADYSRLEETLSKVPV
                     DLSIYTNESVAKLEKLLDSIDYDLDITQQDKLDKLVNEIDKAISDLVAIPLDDDKDNG
                     KDTEKPKEPETPKEPTKPGEPEEPTDVEPEQDKDKLPKTGETLFNMASVGIGLLLIAL
                     IMVKNRKRADYKNMDK"
BASE COUNT          448 a          304 c          233 g          713 t
ORIGIN      
        1 tagagtttat atatcaaaat aaatttgaga ccctagagga actagaatta caattagcag
       61 aatatattta ctggtataat catataagga ttcatggctc cttgggctat atttcaccag
      121 tagaatatag atatagagag aacttaaaat tagtagcata agtgatattg agaaagtata
      181 ttcttgttcg gagtgaattt gtctaaaaag gggttgccaa tccaaattga atatttgtac
      241 atcagtctag aaatgattaa ttacatctaa aacataagac tgtttatatt ctcgcatatc
      301 ggaactattt caaatttaag gaataattac attttaattt tgtcttatga gcaaccctgt
      361 tagaaatttt cacattagat aaatcatact aaaacaggtc aaaggattat aatcccttga
      421 cctgttttta tagggctgct tatcatcttg aaactgtccc atttaatata ttatttatcc
      481 atatttttat agtctgccct ctttctattt ttaaccatta ttaacgctat tagtagtagt
      541 ccgattccta cagaggccat attaaataac gtttctcctg tttttggtaa tttatcttta
      601 tcctgctccg gctctacgtc tgttggttct tcaggttctc ctggctttgt tggctctttt
      661 ggtgtttcag gttctttagg tttttcagta tcttttccat tatctttgtc atcatccaac
      721 ggtattgcca ctaaatcgct tatagcttta tctatttcat ttactaattt atctaatttg
      781 tcctgttgtg ttatatctaa atcataatct attgaatcca ataatttctc taatttagct
      841 acagattcat tcgtgtatat acttaggtct acaggtacct tcgatagtgt ttcctccagc
      901 ctagaatagt ctgctctttt tattattaaa gaatctcttg ctatttcgaa ttcttcttta
      961 agcttttgga attcttgttt tggtgtatta cctatttcta gctccataat tgcttcgtat
     1021 gcttctaaat aagctttata tgtttcttcc gtgtaaattt ctggattctt taagactatg
     1081 gtatttctat aatcttctaa ttcgtcagca ttctgtacta atccgtttat agcagtttca
     1141 aggttctcca atgctttttc aacagattct ttacttccat tgattatatt atcattagct
     1201 tccaataatg attgttccaa tttattgtaa ctgtcccttg tatagagttc tgaatcatat
     1261 ttttcggcct tatgaatcat ttccttaagt gaccttacgt tgactagtcc atttctcttg
     1321 ttaagcattt cctggatgtt tgagcgattt atttccggat tctttaattc aagatcttta
     1381 attgcttttt ctatagcatc ctgcagttct ctatagcttt tctctgtata atcttcttct
     1441 cttattaatt tcatttcctc aattacatct cttatgtctg cgtcttcata tataactaga
     1501 ctttcaatcg catttctaag tatttctgta gcctcattga cttcttcttg cgtcccgtcc
     1561 ttgattacct tttttgcgtt atctagtgaa tcaatatagt tttgccagct ttgtttagta
     1621 tagttatctg ggttaaattc caaatccata agctcattca attctgatat tattactaat
     1681 tccttaatag catctttg
//
LOCUS       DATGSI010000241         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_537522, whole genome shotgun sequence.
ACCESSION   DATGSI010000241 DATGSI010000000
VERSION     DATGSI010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537522"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VK031_03715"
     CDS             complement(<1..305)
                     /locus_tag="VK031_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HLS53054.1"
                     /translation="MIYSVEYADKEIEFKLIRKSVKNINLRVKADMTVVVSANHTVPL
                     GVIMDFVKEKSPWILKHLEKFEKVQPISKSKKEYVSGESFRYLGRQYRLKVFESDTE"
     gene            complement(319..>2494)
                     /locus_tag="VK031_03720"
     CDS             complement(319..>2494)
                     /locus_tag="VK031_03720"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HLS53055.1"
                     /translation="GSGKSLTMTMFSRYVLSELRESSPKLVVVTDRINLDKQIHQTFN
                     HTRLKASRAATGRHLIELINDSGTDIITTLVHKFDTAAEHQKPVLSKDIFVLVDESHR
                     SQYGEMHLKMKKVFPNACYLGFTGTPLMKEEKNTMIRFGELIHTYTIADAVKDKTIVP
                     LLYEGKMIAQTVNRKAIDRRLEMITRKLNDKQKEEVMKKWSRFEKIASSDQRLGLIAF
                     DINEHFLKNYKTQGSQFKAMLATNSKVEAIRYYDEFQSLGDLNAAVVISPPDQREGHS
                     EVDEISKDMVTRFWTKMMDKYGNEENYEETIKNEFVAGDEVDILIVVDKLLTGFDAPR
                     ATVMYIDKPMKEHTLLQAIARVNRLYEGKDYGIIIDYRGLLDKLDEAMEMYSGAGLEK
                     FDPEDLKGALYDVISIVGSLRSYYTELTNIFIRIKNKDDMEEYQVLLEDEELREHFYN
                     TLSSFGRNLSIALESERIYEALSQDEIDRYKKDFRFYQELRKAVKLRYSDTIDHKEYE
                     AKMQNLMDNYIAAEDVLRITNPVDILDEKGFERELERLGTPRGKADAIRTRLTRSIQG
                     KWEENPAYYKKFSERIEETLEAYKERRISEKEYFERMKKLKKDYQDGDSGIEYPETIK
                     YDTGAQPYYGTAMDMFNESNESYEGTEDTAQLAIEIKKIIEKHVQVDWQDNVDIHNRI
                     RQELDDLIFDFKDQKRLNLNIDQIDRMIEEIIKISLNRYGKY"
BASE COUNT          662 a          562 c          332 g          938 t
ORIGIN      
        1 tattcagtat ctgattcaaa aactttcaac ctatactgtc tgcctaaata tctaaaactt
       61 tctccgctta catattcctt tttactttta cttataggct ggactttttc aaatttttcc
      121 aaatgtttta agatccaagg agacttttcc tttacaaaat ccataattac tcctaagggt
      181 acagtgtgat tggcagatac aaccactgtc atatctgcct tcacccttag gtttatattt
      241 tttactgact ttcttattag cttaaactct atctccttat ctgcatactc tacgctgtat
      301 atcatatctt gtctcctact aatacttgcc atatctattt aaagatattt taataatttc
      361 ctctatcatt ctatctattt ggtctatatt caaatttaat cttttttgat ccttaaaatc
      421 aaagattaaa tcatctagct cctgccttat cctgttatga atatcaacat tatcctgcca
      481 atctacctgc acatgttttt ctataatttt cttaatttca atggccagtt gggctgtatc
      541 ttctgtcccc tcataggatt cattactttc attaaacata tccatggcag ttccataata
      601 gggttgggcc cctgtatcat actttatggt ttctggatat tctatacccg agtccccatc
      661 ctgataatcc tttttcagtt tcttcattct ttcaaaatat tccttttcac taatccttct
      721 ttccttatag gcctccaggg tttcctctat tctttcggaa aactttttat aataggcagg
      781 attttcctcc catttccctt gtattgatct ggttagtcta gttcttatgg catcagcctt
      841 gccccttggt gttcctagcc tttctagctc tctttcaaag cccttctcat ccagaatatc
      901 tactggatta gtaatcctta atacatcttc tgcagcaata taattatcca ttaaattttg
      961 catcttggct tcatattcct tatggtctat ggtgtcggaa taccttagtt taactgcctt
     1021 tctaagctct tgataaaatc taaaatcctt tttataccta tctatttcat cttgggacag
     1081 ggcctcataa atcctttctg attctagggc aatgcttaga ttcctaccaa aactacttag
     1141 ggtattataa aaatgctctc tcaactcttc atcttcaagt aacacttgat actcttccat
     1201 gtcatcttta tttttaatcc taatgaaaat attggtaagc tcagtataat aacttcttaa
     1261 agaacctact atgctgatta catcgtaaag agctcccttt aaatcttctg gatcaaactt
     1321 ttcaagacca gcgccagaat acatttccat ggcctcatct aacttatcca aaagtcctct
     1381 ataatctata ataataccat aatccttacc ttcataaagc ctatttaccc tggcaatggc
     1441 ctgtagcaag gtatgctcct tcataggctt atctatatac ataactgtag cccttggagc
     1501 atcaaaacct gtcaatagct tatctactac tattaatata tctacttcat ctccagccac
     1561 aaattcattt ttaatagttt cctcatagtt ttcttcatta ccatatttgt ccatcatttt
     1621 tgtccaaaat ctggtgacca tatccttgga aatttcatca acctcactat ggccttccct
     1681 ctgatctggt ggagatatga ccactgccgc atttaagtct cccaaagact gaaactcatc
     1741 ataatacctt atggcctcaa ccttactatt ggtagccagc atggccttaa attgagaacc
     1801 ttgagtcttg taatttttga gaaaatgttc attaatatca aaggcaatta gccccaatct
     1861 ttgatctgaa gaagctattt tttcaaagcg gctccatttt ttcattacct cttctttttg
     1921 tttatcattt aactttctag ttatcatctc tagccttcta tctatggcct ttctgtttac
     1981 agtttgggct atcatctttc cctcataaag caggggaact atggtcttat cctttaccgc
     2041 atcggctatg gtataggtat ggataagctc tccaaacctt atcatagtat ttttttcttc
     2101 cttcatcaaa ggagttccag taaagcctaa ataacaggca ttgggaaata cctttttcat
     2161 ttttaaatgc atctcaccat attggcttct atgggattca tctactaaaa caaatatgtc
     2221 ttttgaaagg acaggttttt ggtgttctgc tgctgtatca aacttgtgga ctagggtggt
     2281 tataatatct gtccctgaat catttataag ttctatcagg tgcctgcctg ttgcagcccg
     2341 gctagccttt agcctagtat gattaaaggt ctggtgaatt tgcttgtcta aattaatcct
     2401 atctgttacc acaactagct tgggacttga ttctcttaac tccgatagga catacctgga
     2461 aaacatagtc atggtcaaag attttccaga acct
//
LOCUS       DATGSI010000242         1112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_585554, whole genome shotgun sequence.
ACCESSION   DATGSI010000242 DATGSI010000000
VERSION     DATGSI010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585554"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VK031_03725"
     CDS             <1..347
                     /locus_tag="VK031_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53056.1"
                     /translation="AELLGSNRMKQFLQKAKEKFDMIVIDAPPVGLFTDGAILSTLVD
                     GVIFVAAAGEADIETTQRAKERLDKVNANILGVVLNKVEMDSRAQSGYVHYGQASFHN
                     KSAAKRKKRRRK"
     gene            344..>1112
                     /locus_tag="VK031_03730"
     CDS             344..>1112
                     /locus_tag="VK031_03730"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HLS53057.1"
                     /translation="MIDIHSHILPAVDDGAVDLDMALDMASLYVENGFKQVICTPHFM
                     EDLDNFLPEENQKILNKLNEEISKAGLDLNLYLGNEVYISLSLLEDLHKGKFSTLNNS
                     KYILIELPMFNIPLYTEDILYELQVRGYIPIIAHPERNSKIIKDPNILYNFVKDGALA
                     QLNLPSLKGTYGDEIKDTTKVLLQHKLFHFVGTDAHSNGRLSPKVASVLKNLKDLVDE
                     DYFYQLTKLNAKLLLENKDIIKSEPRKIEKKQGFFRRL"
BASE COUNT          393 a          160 c          235 g          324 t
ORIGIN      
        1 cagctgagtt attgggctca aataggatga agcagtttct gcaaaaagca aaggaaaaat
       61 tcgatatgat agtaatagat gcacctcctg taggcttgtt tacagatggg gccattttgt
      121 ctactttagt tgacggagta atttttgtgg ctgctgccgg agaagcagat attgaaacaa
      181 cgcagagagc taaggaaagg ttggacaagg ttaatgcaaa tatattaggt gtggtcttaa
      241 acaaggtgga aatggatagt agggcccaat ctggctatgt tcactatgga caggcaagtt
      301 ttcataataa atcagctgct aaaagaaaga aaaggagacg gaaatgatag atatacattc
      361 ccatatacta ccagctgtag atgatggggc agtagatcta gatatggcct tagatatggc
      421 cagcttatat gtggaaaatg gttttaaaca ggttatatgt actcctcatt ttatggaaga
      481 tttggacaat tttttacctg aagaaaacca gaagatttta aacaagctaa atgaagagat
      541 atctaaggca ggtttagatt taaatctata tttaggcaat gaagtatata tatctctaag
      601 tcttttagaa gacttgcata aggggaagtt ttccacactt aacaatagta aatatatact
      661 gatagagttg ccaatgttta atattcccct atatacagaa gatatattat atgagttgca
      721 ggtaagaggc tatataccaa ttattgccca tcctgaaagg aattcaaaaa taattaagga
      781 tccaaatatt ctatataatt ttgtaaagga tggagccttg gctcagttaa atctgcctag
      841 cttgaaagga acctatggag atgaaataaa ggatacgaca aaggtacttc tccagcataa
      901 gctatttcat tttgtaggga ccgacgccca ttccaatggt agactttcgc caaaggttgc
      961 ctctgtccta aaaaatttaa aagacttggt ggatgaagat tatttctatc aattgactaa
     1021 gctaaatgct aagcttcttt tagaaaataa ggacataata aaaagtgaac ctaggaaaat
     1081 agaaaagaag caaggttttt tccgtagatt at
//
LOCUS       DATGSI010000243         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_113459, whole genome shotgun sequence.
ACCESSION   DATGSI010000243 DATGSI010000000
VERSION     DATGSI010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113459"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VK031_03735"
     CDS             complement(<1..223)
                     /locus_tag="VK031_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HLS53058.1"
                     /translation="MTIHADSNKLNHFIEVGVDSKGNKYLVIHSGSRNLGKQVAEIYQ
                     RLAVDLCSGKEEYFIKRDQIIERFKAEGRR"
     gene            complement(368..856)
                     /locus_tag="VK031_03740"
     CDS             complement(368..856)
                     /locus_tag="VK031_03740"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HLS53059.1"
                     /translation="MKAIYKEFYWKSFLTTRRRSTQFKSNFKTIIWSCYKREFRERLV
                     ELGMSKENKSITADIPNCKYNIPAFWRGFLDGDGSIGMTRNNEPFISVTIRSGKLAKK
                     YLEMLRERFNIIKILNPNKRDNVNNIVVKNEDAISLGSYIYRDSGIHLERKYKKFKEI
                     KK"
     gene            complement(1251..1679)
                     /locus_tag="VK031_03745"
     CDS             complement(1251..1679)
                     /locus_tag="VK031_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HLS53060.1"
                     /translation="MELHGKFGTAKVFTENLEDGAKDQIITLLNQDFIEGSKVRIMPD
                     VHQGMGCVIGFTADMGDKVIPNIVGVDIGCGMLTVELGNIDIDLPRLDKIIHNKIPSG
                     RHVHPGRKYRYEKLQDLHCIREIKNTRRIERSIGTLGGGK"
     gene            complement(1774..>2202)
                     /locus_tag="VK031_03750"
     CDS             complement(1774..>2202)
                     /locus_tag="VK031_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLS53061.1"
                     /translation="LETLFGLKRIGEEGEFIRLKSSASIGNTIDIRTILRGKGINSVG
                     TVHHIAWRAADEDELKKWQSFIKEQGFTVTSVRDRNYFKSIYFREKGGILFEIATDGP
                     GFAIDEDIDSLGEQLMLPSQYENLRQQLEQSLTPIHINKQ"
BASE COUNT          571 a          506 c          284 g          841 t
ORIGIN      
        1 ttcttctacc ttctgccttg aatctttcta ttatttgatc cctctttatg aaatattctt
       61 ctttcccact gcataaatct acagccaagc gttggtatat ttctgccacc tgtttaccaa
      121 gatttcgact tcctgaatgt attaccaagt atttgttacc cttgctatcc actccaactt
      181 caatgaagtg attcaatttg ttactatctg catggattgt cacattgctt taatctccag
      241 agtcccattt ttacagactt ttctgtcctg tctaataatc tcatactatc ttcaacacta
      301 tggttaatat aatggcatct tcctcaggtg tccatgttct tgcattgatt ttaggctttg
      361 ttcttttcta cttctttatt tctttaaact tcttatactt tctttcaaga tgtatgcctg
      421 agtctctata gatgtaacta cctagactaa ttgcgtcttc attctttacc actatattat
      481 ttacattatc ccttttattg ggatttaata tttttataat attaaatctt tcccttagca
      541 tttctaaata cttcttagct aattttccac ttcttattgt cacacttata aaaggttcat
      601 tgtttctagt catgcctatg cttccatctc cgtctaagaa tccgcgccag aatgctggta
      661 tattatactt acagttgggg atatctgcgg taattgactt attttccttt gacatcccta
      721 attcaactag tctctcccta aattctcttt tataacagga ccatattatt gttttaaagt
      781 tacttttaaa ttgtgtagat ctccttctag tagttaaaaa ggatttccaa taaaattctt
      841 tgtaaatagc cttcaaaaca tccttatcct tgtaatttaa ctctatttga accttcccct
      901 gtttcttgta ttaaaatata aatgtccatc tgctagtata aatccaaata aataactatc
      961 aaactccctt gttaaatcca ataatatacc aactcgtaac ttccaaatct ccatacagat
     1021 ggataggtca tttctgccta tctctcacct tttaatcaaa gttggatgat ttatatggtg
     1081 agttcagact gtccctttac ccacatcact atagctgttt gggtagccta ttacagtcgt
     1141 tgcacctccc tataaattat ataggtttgg ctcaggattt ccctttcatc ctaaggggtt
     1201 cccctgaatt taaggctttt attttgacat gtttccatga aaggaggcaa tcacttacct
     1261 ccgccaaggg taccaatact tctttcgatt ctcctggtat tctttatttc tctaatacag
     1321 tgtagatcct gcaatttctc gtatctatat tttcttcctg gatggacatg cctgcctgaa
     1381 ggaattttat tgtgaattat tttatccagt cttggcagat caatatctat attccccagc
     1441 tcaactgtta acataccaca tcctatatcc acgcctacaa tatttggtat taccttgtcc
     1501 cccatatctg ccgtaaagcc gattacacag cccattcctt ggtggacgtc aggcattatt
     1561 cttaccttag atccttctat gaaatcctga tttaaaaggg taataatctg atctttggcc
     1621 ccgtcctcaa gattttctgt aaagaccttg gctgtaccaa actttccatg tagctccaat
     1681 ctatctcccc tttcataaat aatctcactt atttacacct atattttatc acagttgttc
     1741 cttagtgcaa aattaggacc ttcataagcc ctattactgc ttatttatat gaataggtgt
     1801 tagactttgc tctaactgct gccttaaatt ttcatactgg cttggaagca ttaattgttc
     1861 tcctaaagag tctatgtcct catctattgc aaatccaggt ccgtcagtgg caatttcaaa
     1921 aaggattcca cccttttccc taaaatatat agacttgaaa tagtttctat ctcttacgct
     1981 ggtaacagta aaaccctgct ccttaataaa actttgccac tttttcaatt catcctcatc
     2041 tgctgccctc caggctatgt ggtgcactgt tcccacgcta tttattcctt tgcctcttaa
     2101 tatagttctt atatctatgg tatttccaat ggaagcagag gatttaagcc taataaactc
     2161 tccctcttcc cctattcttt ttagtccaaa caaggtctct aa
//
LOCUS       DATGSI010000244         1450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_490078, whole genome shotgun sequence.
ACCESSION   DATGSI010000244 DATGSI010000000
VERSION     DATGSI010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490078"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VK031_03755"
     CDS             <1..800
                     /locus_tag="VK031_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HLS53062.1"
                     /translation="TFGGINLEDISAPRCFEIEQKLKEKLDIPIFHDDQHGTAVVVSA
                     GLINALKIVDKKMEDIKVVVNGSGAAGVAIVKMLLALGVDDIIMCDSRGIIYKDSPTN
                     NWIKEEMAEVTNKEDIRGDLKDAMVDADVFIGVSIANIVDEEMVKSMKEDPIIFAMAN
                     PVPEIFPEDAKRAGAKVVGTGRSDFPNQINNVLAFPGIFRGALDARASDINDEMNVAA
                     AYAIAGMVKESDLTADFVIPDALDKAAADAVAEAVKKAAIESGVSKL"
     misc_binding    848..1042
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1086..>1450
                     /locus_tag="VK031_03760"
     CDS             1086..>1450
                     /locus_tag="VK031_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HLS53063.1"
                     /translation="MAETMGSLRRSNMCGELRAKDIGKEVVVMGWVQKERNLGSLIFA
                     DLRDITGIVQVVFDETLPEEIFKKAEKIRTEYVLAVKGMVREREAENKNIPTGQVEIL
                     AQDVKILDKAETPPIYIRD"
BASE COUNT          516 a          199 c          353 g          382 t
ORIGIN      
        1 caacctttgg aggaataaat ctggaagata tttctgctcc tagatgcttt gaaatagaac
       61 agaaattaaa agaaaaatta gatataccca tattccatga tgatcagcat ggaactgctg
      121 ttgtggttag tgcaggccta ataaatgcct taaagattgt cgataagaaa atggaggaca
      181 taaaggtcgt tgtaaacggt agtggggcag caggagttgc aattgtaaaa atgttattgg
      241 ccctaggtgt agacgatatt attatgtgtg atagtagagg aattatatat aaggattctc
      301 caactaataa ctggattaaa gaagagatgg ctgaagtaac caataaggaa gatattaggg
      361 gagacttaaa ggatgccatg gtagatgcgg acgtatttat aggtgtctcc attgctaata
      421 tagtagatga agaaatggtt aaatctatga aagaggatcc aattatcttt gctatggcta
      481 acccagtacc tgaaatattc ccagaggatg ctaaaagagc tggggctaag gttgtaggta
      541 caggaagatc agattttcca aatcagatca ataacgtgct ggccttccct ggaatcttta
      601 gaggagcctt agatgcaagg gctagtgata taaatgatga aatgaatgtg gctgccgcct
      661 atgctatagc aggaatggtt aaggaatcag acctaacggc agattttgta ataccagatg
      721 cactagataa ggctgctgca gatgcggttg cagaagctgt taagaaggcc gccatagaat
      781 caggagtttc aaaattataa tcaatattga cagcaaaaat aatttataat ataattaatt
      841 aaattaataa tttgcaatga taaggagaag tattaagtca cttagtccca gagagttggc
      901 actggtggaa agccagcact aatacttaat atagacacct tggagcaaac aatttgaaaa
      961 aattgttcgc caagtgcgtt aaacttttaa gtgaggatta cctaattagg gtggtaccgc
     1021 gagataacct ctcgtccctg aatatcagag atgagaggtt aaatttatgt aggaggtaaa
     1081 ctgaaatggc ggagacaatg ggaagtttaa gaagaagcaa catgtgtggt gagctaaggg
     1141 ctaaagatat aggaaaagag gtagttgtaa tgggctgggt ccaaaaagaa aggaacttag
     1201 gctcattaat atttgctgat cttagagata taacaggaat tgttcaggta gtatttgatg
     1261 aaaccttgcc tgaagaaatc tttaagaagg ctgaaaaaat aaggacagaa tatgttttag
     1321 ctgttaaagg aatggtaagg gaaagggagg cggaaaataa aaacatccct actggacagg
     1381 ttgagatcct agcccaggat gtaaagatct tagataaggc tgaaactcca cctatctata
     1441 ttagagatga
//
LOCUS       DATGSI010000245         3204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_585854, whole genome shotgun sequence.
ACCESSION   DATGSI010000245 DATGSI010000000
VERSION     DATGSI010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585854"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1809)
                     /locus_tag="VK031_03765"
     CDS             complement(<1..1809)
                     /locus_tag="VK031_03765"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HLS53064.1"
                     /translation="MLEKLIHTVEQYNPQADLNLIIEAFTYGEAAHEGQFRNSGEKYF
                     IHPFNVAMILADLNMDTSTIVAGLLHDVIEDTEVTYEDVVDKFGKEIADLVDGVTKLK
                     KLKYKTKQESQAENLRKMVLAMAKDIRVIIVKLADRLHNMRTLEYMTDRKRKEKAIET
                     LEIYAPIAHRLGMSRIKWELEDLSLRFLEPEKYYELVEKVSSKRKEREARIQEIINTL
                     KDKLEEMGIECQISGRPKSFYSIYKKMEIQGKSFEQIFDLTAIRVLVNNIKDCYGVLG
                     IVHTLWKPLPGRFKDYIAMPKPNMYQSLHTTVIGFKGEIFEVQIRTYDMHKTAEYGIA
                     AHWKYKEGIKKAENFDKKLTWLRQLLEWQNDLKDPKDFMETLKIDFFTDEVFVFTPMG
                     DVINLPEGSTPIDFAYRVHTDIGNSCVGAKVDGRIVPLNYRLKNGNIVEIITSPNSTG
                     PSRDWLKIVKSTQAKSRIRQWFKLKDRDTNIVKGKEALEKELKRQGYNLNDILREDWL
                     NQISSKLSLNSIDDLYASVGYGNIRLNQIITRLREFYNKEFEEEHREHYVKEKMKQPA
                     RKPRPRSSQGVSIRGVDNIKVRFAKCCNPVPGDEIIG"
     gene            complement(1845..>3204)
                     /gene="recJ"
                     /locus_tag="VK031_03770"
     CDS             complement(1845..>3204)
                     /gene="recJ"
                     /locus_tag="VK031_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HLS53065.1"
                     /translation="TIITCDNGIAAIEAIDHAKKVGIDVIVTDHHNLSFVEEDGEKRC
                     IVPAAVSIVNPKQPDCPYPFKGLCGAGVVYKLVEYLFEIYNLPKEKSYKLLEYVAIAT
                     ICDVVDLVDENRIIVKHGLKQINATKNLGFKTLVDVLGIKNEIGTYHIGFIIGPTLNA
                     SGRLDTAYLALELLLTEDKNKAIGLAKELKALNEDRKAITEKGIKLVVEKIENTGIKN
                     DKVLIIYEPKIHESVAGIVAGRVKEIYNKPTIVLTEGLDSIKGSGRSIEEYDMFKELV
                     RCKDILDKFGGHPMAAGLSLKADNIELLRTRLNKNTDLTENDLIKKIYIDMPLPIEYI
                     NYKLINQINGLEPFGKGNPRPLFGDRGLKIKQAFKLGANKRTIKLILQSKKGISIEGL
                     LFNGADKFEETIITKYGRKELENMYEGYSNNIILDILYYPSINEYMNRRSIQVIIQNY
                     RV"
BASE COUNT          883 a          695 c          412 g         1214 t
ORIGIN      
        1 gcctataatt tcatcacctg gcaccggatt acaacatttg gcaaacctta cctttatatt
       61 atctactcct ctaatagata cgccctggct acttcttggt ctaggctttc tggcaggttg
      121 tttcatcttt tccttcacat aatgctctct gtgttcttct tcaaattcct tattataaaa
      181 ttcccttaac ctggttatta tttgattaag cctgatattc ccatagccaa ctgatgcgta
      241 caaatcatca attgaattta agcttaattt agaggaaatt tggtttagcc aatcttctct
      301 taatatatca tttagattat aaccctgtct cttaagttcc ttctctaggg cctctttacc
      361 ttttacaata ttagtatccc tatcctttaa tttaaaccat tgcctgatac gtgatttagc
      421 ctgggtactt tttactattt ttaaccaatc tctactaggc cctgtactat ttggagaagt
      481 gattatttct actatattac catttttaag tctatagttt aagggtacaa tcctaccatc
      541 aaccttggca cctacacaac tatttcctat atctgtatgg accctataag caaaatcgat
      601 aggtgtcgat ccttcaggca agtttattac atcacccatc ggtgtaaaaa caaatacttc
      661 atctgtaaag aaatctatct ttaaagtttc cataaagtcc ttaggatcct ttaggtcatt
      721 ttgccactct aataactgtc ttaaccatgt tagtttttta tcaaagttct cagccttttt
      781 gatcccttcc ttatatttcc aatgggctgc aataccatac tctgctgttt tgtgcatgtc
      841 gtaggttcta atttgcactt caaatatttc acccttaaag cctattaccg ttgtatgaag
      901 agattgatac atgttaggtt taggcatggc tatataatct ttaaacctgc caggtaatgg
      961 tttccaaagt gtatggacta tgcctaatac tccataacaa tcctttatat tattaaccaa
     1021 cacccttatg gcagttaaat caaatatctg ttcaaaagac ttgccttgta tttccatttt
     1081 cttatagatg ctataaaagc tttttggacg gccactgatt tggcattcaa ttcccatctc
     1141 ttctagtttg tcctttaagg tattaataat ttcctgtata cgggcctctc tttcttttct
     1201 tttgctagaa accttctcta ccagctcgta atacttttct ggctctagaa atctcaggga
     1261 caaatcctcc agctcccact tgattctaga cattcccagt ctatgggcta taggtgcata
     1321 tatttccaag gtttcaattg ccttttcttt tctcttcctg tcagtcatat attctaaggt
     1381 tctcatatta tgcaacctat cagctaattt aacaattata acccgtatat ctttagccat
     1441 tgctaagacc attttcctca aattttcagc ctggctctcc tgttttgtct tgtactttaa
     1501 tttctttaac ttggttactc catcaactag gtctgctatt tctttgccaa acttatctac
     1561 cacatcttcg tatgttacct ctgtatcttc tataacgtca tggagaagac ctgcaactat
     1621 tgtggaagta tccatattta aatctgccaa tatcatggct acgttaaagg gatggataaa
     1681 gtatttctct ccagaatttc taaattgtcc ttcatgtgca gcctccccat aggtaaatgc
     1741 ttctataatt aagttcagat ctgcttgagg attatattgt tctactgtat gaatcaattt
     1801 ttccaacatg gccatcacct ctttctatct atgataagta ataattaaac cctataattt
     1861 tgtataatta cctgtatgct ccttctattc atatattcat ttatggaagg ataatataaa
     1921 atatctaata taatattatt actataccct tcatacatat tttctaattc cttcctacca
     1981 tattttgtaa ttatggtttc ctcaaactta tcagcaccat taaataatag tccttctatc
     2041 gaaataccct ttttagactg gagtatgagc ttgattgtcc ttttatttgc acctaactta
     2101 aatgcctgct ttatttttag ccccctatcc ccaaacaaag gcctaggatt acccttacca
     2161 aagggctcca aaccatttat ctggttaata agcttgtaat taatatattc aataggaagg
     2221 ggcatgtcaa tataaatctt tttaatcaaa tcattttcag ttaaatctgt atttttattt
     2281 aaccttgtcc ttaaaagctc tatattatct gcctttaaag aaagtccagc cgccatagga
     2341 tgtccaccaa atttgtctaa aatatctttg cacctgacta attctttaaa catatcatat
     2401 tcctctatgg acctacctga acccttaatg gagtctaacc cttcagttaa tactatagtc
     2461 ggcttattat atatttcttt aactctaccg gccactatac ctgccacact ttcatgtatt
     2521 ttaggttcat atattattaa taccttatca ttttttatcc ccgtattctc tatcttttca
     2581 actaccagct taattccctt ttctgtaata gcttttctat cctcatttag agcttttaat
     2641 tcttttgcca gccctattgc cttattctta tcttcagtaa gtaaaagttc caaggccaaa
     2701 taggctgtat ccaatctacc tgaggcattt aaggttggtc ctattataaa gcctatatga
     2761 taggttccta tttcattttt tatacccaat acatcaacaa gagtcttaaa tcctagattt
     2821 tttgtggcat ttatctgttt caagccatgt tttacaataa ttctattttc gtcaaccaaa
     2881 tctactacat cacatatggt ggctatggcc acatattcta acagtttgta gcttttctcc
     2941 ttaggaagat tatatatttc aaataaatac tctacaagtt tatataccac acctgcccca
     3001 cataagcctt taaatggata gggacagtca ggttgtttag gatttacaat actaactgca
     3061 gcgggaacaa tacatctttt ttctccatcc tcttctacaa aagataggtt gtggtgatct
     3121 gttacaatca catctatacc taccttttta gcatgatcta tagcctctat tgctgcaatg
     3181 ccattatcac aggttattat ggta
//
LOCUS       DATGSI010000246         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_113585, whole genome shotgun sequence.
ACCESSION   DATGSI010000246 DATGSI010000000
VERSION     DATGSI010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113585"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VK031_03775"
     CDS             <1..605
                     /locus_tag="VK031_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLS53066.1"
                     /translation="ENFLASVSLLSDVDKTIDEDNLITLMTVHSAKGLEFKVVFLVGM
                     EEGLFPISRSLDSDEDLEEERRLCYVAITRAEEQLFITSARRRILYGRWEYSLPSRFI
                     TELGDTIKRKVPTVEKSRLVRTIDRTAPVMPNILGANPVKRNKKDVDINIGDKVKHKK
                     FGQGMVVQIKDKDKDKEITVAFDNQGIKRLLLSVAPIEIL"
     gene            618..2606
                     /gene="ligA"
                     /locus_tag="VK031_03780"
     CDS             618..2606
                     /gene="ligA"
                     /locus_tag="VK031_03780"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HLS53067.1"
                     /translation="MDKKLRIQELIEIINDLNYYYYTLDSPKVSDKEYDQLYDELVSL
                     EKETGIVYDHSPTQRVGGQILDKFEKHTHLARLWSLDKSQSFDELRNWDRRVRRLIQE
                     HNNNREDKLPSPEYILEYKFDGLTINLTYRDGKLIQGATRGNGTVGEAILEQIKTIRS
                     IPLQIDFKGTIEIQGEGLMPLSALEKYNEKAEEPLKNARNAAAGALRNLDTKVTAERN
                     LTAYFYNIGYIEGKEFKKHMEVLDFLRANRLPVFPYAKAFDHMEDLIREIDRLKEDRS
                     KLDLLTDGLVIKINDIKTREILGFTNRFPRWAMAYKFEAEEVSTRLLKVQWNVGRTSK
                     VTPTAILEPVEIGGVTVRRATLNNYDDIVRKRLRLNSRVLLRRSNDVIPEILGTLETE
                     EETFEIEKPVHCPACHSELYQDGVHIFCPNSLSCKPQLVARLVHFASRDAMNIEGFSE
                     KTAEQLLEELNIRDLPEIYEIEFEDLIKLDGFQERKSNNLLNAIERSKNVSLASFIYA
                     LGINNVGIKTATDLADYYKSIDALKEASYDELVQIGDIGPIIAESILEFFQDEEILKA
                     IDKLLSEGVNPTYEEIEEEEGLFTGKTLVITGTIEGYTRKELRERVEAMGGRVTGSVS
                     GRTDYLIAGENPGSKYSRAVELGVEIINEERLKEILED"
BASE COUNT         1025 a          384 c          645 g          692 t
ORIGIN      
        1 tagaaaactt cttagcatca gtatctttgc taagcgatgt ggataagact atagatgaag
       61 acaatctgat tactttaatg actgttcata gtgcaaaagg attggaattt aaagtagtat
      121 ttttagtagg catggaggag gggctattcc ctatttccag atcccttgac agcgatgagg
      181 atttggagga agaaagaagg ctttgttatg tggccataac cagggcggag gaacaactat
      241 ttattactag cgccagaagg agaatcctat atggaagatg ggagtattct ctgccttcca
      301 gatttataac tgaattagga gatacaatta aaagaaaggt accaactgta gagaaaagtc
      361 gtctagttag aaccatagac aggactgccc cggttatgcc taacatttta ggagccaacc
      421 ctgtaaaaag aaacaaaaaa gatgtggata taaatatagg agataaggtg aaacataaaa
      481 agtttggaca gggtatggtg gttcagatta aggataagga taaagataag gaaataactg
      541 tagcctttga taatcaaggg attaagcgat tactgttatc ggtggcacct atagaaattc
      601 tataagaggt gaatttattg gataaaaagt taagaataca agagctaata gaaataataa
      661 atgatcttaa ttattattat tataccttag acagccctaa ggttagcgat aaggagtatg
      721 accaattata tgatgaattg gtaagccttg agaaggaaac aggtatagta tatgaccatt
      781 caccgactca aagggtaggg ggacaaattt tagataagtt tgaaaaacat acccacctgg
      841 ctagactttg gagtctggat aaaagtcaaa gctttgatga gttaagaaat tgggatagaa
      901 gggtcagacg actaatccaa gagcataata acaataggga agataagcta cctagtccgg
      961 aatatatact ggagtataag tttgatggct tgacaataaa cttaacctat agggatggaa
     1021 aactaatcca aggtgccacc aggggaaatg gaacagtagg agaggctatc ctggaacaaa
     1081 taaaaaccat aaggtctata cccctacaaa tcgactttaa gggtacaata gaaatccagg
     1141 gagaaggact tatgccttta tcagccttag aaaaatataa tgaaaaggca gaagaacctt
     1201 taaaaaatgc tagaaatgca gcggcaggag ccctaagaaa tctagataca aaggtaacag
     1261 cagaaagaaa cctaacggct tacttctata atatcggcta tattgaggga aaagaattta
     1321 agaaacatat ggaagttcta gactttttaa gagccaatag attgccagta tttccctatg
     1381 ccaaggcctt tgaccatatg gaagacttga ttagggaaat agataggtta aaggaagaca
     1441 gaagtaagct ggacctatta acagatggac tagtaataaa aataaatgat ataaaaacca
     1501 gggaaatttt aggctttacc aataggttcc ccagatgggc aatggcctat aaatttgagg
     1561 cagaagaggt aagcaccaga ttattaaagg ttcaatggaa tgtgggaagg acctctaagg
     1621 taacgccgac tgctatttta gaaccagtgg aaattggagg ggtgacagta aggagggcaa
     1681 ccttaaataa ctatgatgat atagtgagga aaaggttaag gctaaattcc agagttctat
     1741 taagaagatc caatgatgta ataccagaaa tcctagggac cttggaaacg gaagaggaaa
     1801 cctttgaaat tgaaaagcca gttcactgcc ccgcttgcca tagtgaattg tatcaggatg
     1861 gggtccacat attttgtcca aattccctat cctgcaaacc acagctggtg gcccggttag
     1921 tccattttgc cagcagggat gctatgaata tagaaggctt tagtgaaaaa actgcagagc
     1981 agttgctgga agagttaaac ataagagatc tacctgaaat atatgagata gagtttgagg
     2041 atttaataaa gcttgatggt tttcaggaaa ggaaaagtaa taatttacta aatgccatag
     2101 aaagaagcaa aaatgtatcc ttggcatcct tcatttatgc cttaggtatt aataatgtgg
     2161 gaattaaaac agcaactgac ctggctgact attataagtc catagatgct ttaaaagagg
     2221 caagctacga cgaattggta caaatagggg atatagggcc tattatagct gaaagcatat
     2281 tggaattttt ccaggatgaa gaaattttaa aagctataga taaactttta agtgaaggtg
     2341 taaatcctac ttatgaagaa attgaggagg aagaaggttt atttactggc aagaccctag
     2401 taattactgg tactatagaa ggctacacca ggaaggagtt aagagaaaga gtagaggcca
     2461 tgggtggcag ggtaactggc tcagtaagtg gaagaactga ttacctgata gcaggagaaa
     2521 acccagggag taaatacagc agggcagtgg aactgggagt agaaataata aatgaagaaa
     2581 gattaaagga aatattagaa gattaaggtt taaattccta atatagaagg agtgttttta
     2641 tgataaacag ggaaaaaatc ttacaattgg cagatttatg caagttggat gtggaaaaag
     2701 aggtggaaat ctatagggag gaaatatcta atttactaga gacctt
//
LOCUS       DATGSI010000247         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_113603, whole genome shotgun sequence.
ACCESSION   DATGSI010000247 DATGSI010000000
VERSION     DATGSI010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113603"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2031)
                     /locus_tag="VK031_03785"
     CDS             complement(<1..>2031)
                     /locus_tag="VK031_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HLS53068.1"
                     /translation="RRYLAQRAELLGAIRLPNNTFKNAGAEVTSDIIFLQKRDRPIDM
                     DRDWIHLDTDEDGITLNSYFVDNTEMILGKMEMKSGPFGMESTCTPLPDADLSEQLKV
                     AIQNIEGSISEIDLPDMELDTDTSIPADPKVKNFSYTLVDGEIYYRENSRMIKPNTNE
                     RAKERIKGMVELRECVDNLIRYQLEDYSEETIKEEQETLNRLYDSFTAKYGLINSRGN
                     SLAFSDDNSYYLLCSLEDVDENGNLKAKADMFTKRTIKKRTSVHSVDTASEALAVSIA
                     EKARVDMAYMGRLTGLNEEELANDLRGVIFPLPTPLDNDSPTNYVTADEYLSGNVREK
                     LQTAKLALQTSDIYRSNVEALESAQPKDLDASEIDVRLGATWIDKEYIQQFMYELFDT
                     PYRYQGQIEVKYAPFTAEWNISNKNAVGYNNVAAYVTYGTDRANAYKILEDSLNLRDV
                     RIYDTITDADGKEKRVLNKDATTLAQQKQQAIKDEFQDWIWKDADRRQTLVKKYNKKF
                     NSIRPREYDGKHINFVGMNPEITLRPHQVNAIAHILYGDNVLLAHEVGAGKTFEMVAA
                     AMESKRLGLCQKPLFAVPNHLTEQWAGEFLRLYPAANILVATKKGFETRNRKKFCARI
                     ATGEYDAVIIGHSQFERIPMSIERQERLLREQIDEILEGIKEVKASGGERFTV"
BASE COUNT          560 a          443 c          340 g          688 t
ORIGIN      
        1 acagtaaatc tttcaccacc acttgccttt acttctttaa ttccttccaa gatttcatca
       61 atctgttcac gaagaagtct ctcctgcctt tcaatagaca taggaatacg ctcaaactga
      121 ctatgaccta taataactgc atcatattcc cctgtagcta ttcttgcaca aaacttcttt
      181 ctatttcttg tttcaaaacc cttctttgtg gctactaaaa tattagcggc agggtaaagc
      241 cttaaaaact cacctgccca ttgctctgtt aaatgattag gtacagcaaa gagtggtttt
      301 tggcaaagtc ctaacctttt actttccata gcagcagcta ccatttcaaa agtctttcct
      361 gcacccactt catgggcgag aagaacatta tccccataaa ggatatgggc aatggcattg
      421 acttggtggg gtcttaatgt gatttcagga ttcataccca caaagtttat atgctttcca
      481 tcgtattcac gggggcggat ggaattaaat ttcttattat atttcttaac taaggtctgc
      541 cttctgtctg catccttcca aatccaatcc tgaaattcat ccttaatggc ttgttgtttt
      601 tgctgtgcca aagtagttgc atccttattt agtacacgct tttccttgcc atcagcatcg
      661 gttatagtgt cataaatcct aacatcacga agatttagac tatcttctaa aattttataa
      721 gcattggctc tatctgtacc ataggtaaca tatgcagcta cattgttata tccaacggca
      781 tttttattgc taatattcca ttccgcagta aatggggcat atttaacttc aatttgcccc
      841 tgataacggt aaggagtatc aaataattca tacataaact gctgaatata ttccttatca
      901 atccaagtag caccaagacg gacatctatt tcagaggcat ctaaatcctt tggctgtgcc
      961 gattctaaag cttccacatt gcttctataa atatctgatg tttgtagggc aagtttagct
     1021 gtttgaagct tttcccttac attaccagaa agatattcat ctgctgtaac ataatttgta
     1081 ggactatcat tatcaagggg agtaggtaga gggaagataa cacctcttaa atcattggca
     1141 agctcttctt cattaagtcc tgttagccta cccatatatg ccatatctac ccttgctttt
     1201 tcagctatag agactgctaa tgcctcgctt gcagtatcaa ctgaatggac agatgtacgc
     1261 tttttaatgg tacgcttagt aaacatatct gccttggctt ttagattgcc attttcgtct
     1321 acatcttcaa gggaacaaag gagataatag gagttatcat cactaaaggc aaggctatta
     1381 ccacggctgt taatgagtcc gtatttagca gtgaagctat cataaagccg attaagggtt
     1441 tcctgttcct ctttaatagt ttcctcactg taatcctcca actggtagcg tataagatta
     1501 tctacgcact ccctcagctc caccattccc ttaattcttt cctttgccct ctcattggta
     1561 tttggcttta tcatgcgaga gttttcacgg tagtatattt caccgtctac aagggtatag
     1621 gaaaagttct taacttttgg gtcagcaggg atggatgtat cagtatctaa ttccatatca
     1681 ggtaaatcta tttctgatat actaccttca atgttttgaa tagccacctt tagctgttct
     1741 gaaaggtcag catctggaag gggtgtacaa gtactttcca ttccaaaggg tccgcttttc
     1801 atctccattt ttcctagaat catctcagta ttatctacaa agtaactatt caaggtaatg
     1861 ccatcttcat cggtatcaag atgaatccaa tccctatcca tatcaatagg tctatctctc
     1921 ttttgtagaa agataatatc acttgtaacc tctgcacctg catttttaaa ggtgttgtta
     1981 ggtagtctaa ttgcacctaa taattcagcc ctttgtgcaa ggtatctgcg t
//
LOCUS       DATGSI010000248         1231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_538189, whole genome shotgun sequence.
ACCESSION   DATGSI010000248 DATGSI010000000
VERSION     DATGSI010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538189"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VK031_03790"
     CDS             <1..277
                     /locus_tag="VK031_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLS53069.1"
                     /translation="VVSRKDIIRAIMGGTDLFKVPVGIIMTRMPNIVCVKPEDSILIA
                     ANKLIEHEIDSIPVVEYKSDDSKDLIVTGRITKTSITRIFVELGNNI"
     gene            289..1122
                     /locus_tag="VK031_03795"
     CDS             289..1122
                     /locus_tag="VK031_03795"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HLS53070.1"
                     /translation="MNKEISIYVISDSIGETGELIARAAVKQFVSEKDYDIRRYAYIE
                     TEEQVEEIFKEAQEETRSLVIYTTVSERTRKYITKKGIELNIPTIDVMTPPLQALESV
                     LGFPPKRESGIIRRLDENYFNKVAAIEFAVKYDDGKDPRGIKKAEICLVGVSRTSKTP
                     LSMYLAHKYFKVANVPLVPEVPPPRELFEKDRRRVIGLVANPLKLNEVRQERLKSLGL
                     RYNANYADINRINEELEYSRNIMKKLGCIVIDVSNKAIEETAGIVIDHMIDKFGQKSL
                     L"
BASE COUNT          448 a          157 c          273 g          353 t
ORIGIN      
        1 agttgtatca aggaaggata tcataagggc cattatgggc ggtacagacc ttttcaaagt
       61 tccagtaggt ataattatga ctcggatgcc aaatattgta tgtgttaaac cagaagatag
      121 catcttaatt gctgcaaaca aattaatcga acatgaaatt gatagtattc cagttgtaga
      181 atataaaagt gatgattcaa aagatttgat agtaaccgga agaataacta agacaagcat
      241 aacaaggatc tttgtggaac tgggtaacaa tatctagggg gtggttctat gaataaggaa
      301 atcagcatat atgtgatttc tgactcaata ggtgaaacag gcgaattaat tgccagggct
      361 gcagttaaac agtttgtttc tgaaaaggat tatgatatta gaagatatgc ctatattgag
      421 acagaagagc aggtggaaga aatttttaaa gaggctcaag aagaaacccg tagcctggtt
      481 atatatacta cagtatctga gaggacaagg aagtatataa caaaaaaggg aatagagctt
      541 aatatcccta caattgatgt aatgacacca cccttacagg cattggaaag tgtattgggc
      601 tttcctccta agagagaatc tggtataata agaaggctgg atgaaaatta tttcaataag
      661 gtggcagcaa ttgagtttgc tgtaaaatat gatgatggta aggaccctag gggaattaag
      721 aaggcggaaa tctgcttggt tggagtatct agaacttcta aaacgccttt aagcatgtat
      781 ctggcacata aatactttaa ggtagccaat gttcccttag tacctgaggt accaccacct
      841 agggaattgt ttgaaaaaga tagaagaaga gtaattggat tggtggccaa tccgttaaaa
      901 ttaaatgagg tcaggcagga gcgtttgaaa tctctaggtt taagatataa tgccaactat
      961 gcagatataa ataggataaa tgaggaattg gaatattcta gaaatataat gaaaaaatta
     1021 ggttgtatag ttatagatgt atcaaacaag gccatagagg aaactgctgg aattgttatt
     1081 gaccatatga tagacaagtt tggtcagaaa agcttattat agaagtgttg gattcaacac
     1141 ttctttttaa gtctagaaaa atagaataat ttttcaatat aaagaaggat tttttgcttt
     1201 cctgttgaat atatacaatg tattaggtac t
//
LOCUS       DATGSI010000249         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_490343, whole genome shotgun sequence.
ACCESSION   DATGSI010000249 DATGSI010000000
VERSION     DATGSI010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490343"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..526
                     /locus_tag="VK031_03800"
     CDS             170..526
                     /locus_tag="VK031_03800"
                     /inference="COORDINATES: protein motif:HMM:NF015729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HLS53071.1"
                     /translation="MNRLSQLGLVLAILFLSEILQSYFALPIPATIIGMLLLLILLSF
                     KIVRLSWISRISKTLLDNLSIMFVPAGVAIMNELDIFKGHMLAISITIFATTIMVIVV
                     TGYVVQALAEARKGRR"
     gene            529..1224
                     /locus_tag="VK031_03805"
     CDS             529..1224
                     /locus_tag="VK031_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HLS53072.1"
                     /translation="MIDYVNTPLFGVGLTIIVFSIFNYLSKKIKLSLFNPIGLSIISI
                     IYILRYNNIDYELYNRGGSIISFFLGPATVVLAVPLYRRIKLLKDNFFPIMVGILAGS
                     VTGIISVIFFCKFFKLDRLITLSMIPKSTTSAIAVDISRAIGGNPAITIALVTATGIL
                     GNIVGPLIFRIFRIDNQLAKGISLGTASHVVGTAKAMEIGETVGAMSSLAIGLAGLVT
                     VFLAPLILKIINI"
     gene            1244..1720
                     /gene="msrA"
                     /locus_tag="VK031_03810"
     CDS             1244..1720
                     /gene="msrA"
                     /locus_tag="VK031_03810"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HLS53073.1"
                     /translation="MKEIYLAGGCFWGVEEYMSRIAGVVDTEVGYANGHTKNPSYEEV
                     CTGTTGHVEATYVKFDENIISLEELLYKFWNIIDPTVLNRQGPDIGSQYRTGIYYKDK
                     SDLEVINKTLKVVQEKYQEPIVTEIKELKSFYKAEEYHQDYLKKNPDGYCHIDLSK"
     gene            1790..>2473
                     /locus_tag="VK031_03815"
     CDS             1790..>2473
                     /locus_tag="VK031_03815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HLS53074.1"
                     /translation="MVDVKADLEDLQMEIIKQLPDLIENSKKKALEFMNNGKVDFDRE
                     IILKGNIKGLNKLYTSDNIYAMKDLLDRGYKGKIDLIYIDPPFFTMANYWHRISLGEK
                     NIRYLAYNDIWKDGLLEYLEMLTVRLYLMKELLSSRGTIYVHLDYRTVHYVKIIMDCI
                     FGRERFLNEIIWAYRSGGASRRHFARKHDTILAYTKTEEYIFNPQKEKSYNRGLKPYR
                     FKGVKEYQDE"
BASE COUNT          875 a          309 c          514 g          775 t
ORIGIN      
        1 agaaaggcca ggaagaaatg acttttgaac tggagaatca agaaataaaa ttaaaacttc
       61 tttttaatga tatatcaggc agggttaatg aaagagggaa gttgcttata gaacatataa
      121 gttatatatt atttattaag attaaataat ataaggaagg aagataaggg tgaatagatt
      181 aagccaattg ggactggtgt tagcaatatt atttttaagt gaaattcttc agagctactt
      241 tgctttgcca ataccagcca caataatagg catgttactt ttattaattc tactaagttt
      301 taaaatagtc aggttatcct ggatatccag gatatccaaa accttgctgg acaacctatc
      361 tataatgttt gtacctgcgg gagtggctat tatgaatgaa ttggatatat ttaaaggtca
      421 tatgctggct atttccataa ccatatttgc tacaacaatt atggtaattg tagtgacagg
      481 atacgtggta caagctctgg cggaagcaag gaaaggtagg aggtaagaat gatagattat
      541 gttaatactc ccttatttgg tgtagggctg acaattatag ttttttcaat ctttaattat
      601 ctaagcaaaa aaattaagct ttcacttttt aaccctatag gcttaagtat tataagtatt
      661 atttatatat taaggtataa taatattgat tatgaattat acaatagggg aggcagtata
      721 attagctttt tcttagggcc tgcaacagtg gtattggcgg tgcccttata tagaaggata
      781 aaattattaa aagataactt tttccccata atggtaggaa tccttgctgg ctctgtaact
      841 gggattatat ctgtaatctt tttttgtaag ttctttaagt tagatagact tataaccttg
      901 tccatgatcc caaagtctac aacctctgcc attgcagtag atatttccag ggccataggt
      961 ggaaacccag ccataaccat tgccttggta acagctacag gcatactggg aaatattgta
     1021 ggacctttga tttttcgtat atttaggata gataatcagt tggctaaagg tatatcttta
     1081 ggaacagcct cccatgtggt aggtacagct aaggccatgg aaatagggga gacagtagga
     1141 gccatgagct ctctagccat agggctagca gggttggtta ctgttttttt ggcaccttta
     1201 atattaaaga ttattaatat ataaaaaaga gaggatgaaa agaatgaagg aaatttactt
     1261 ggcaggagga tgtttttggg gagtggaaga atatatgtct aggatagctg gcgttgtaga
     1321 tacagaggta ggatacgcca atgggcatac taaaaatcct agttatgaag aggtatgtac
     1381 aggtactaca ggacatgtgg aggccactta tgtaaagttt gatgaaaata ttatctcctt
     1441 agaagagctt ttatataaat tttggaatat tattgatcct actgttttaa ataggcaggg
     1501 acctgatata ggaagccaat atagaacagg gatctattat aaggataaat cagacttgga
     1561 agttataaat aaaactttaa aagttgtaca agaaaagtat caagagccta ttgtaacaga
     1621 aattaaagag ttaaagtcct tttataaggc tgaggagtac catcaggatt atctaaaaaa
     1681 gaatccagat ggttattgcc atatagattt aagcaagtaa gaacctaaat aataggttct
     1741 ttttttaatg gaggccttat ttatgtaaaa ggtatgaaag aatgatataa tggtggatgt
     1801 aaaagcagac ttggaggatt tacaaatgga gattataaaa cagcttcctg atttaataga
     1861 aaatagtaag aaaaaggccc tagaatttat gaataatggg aaagtagact ttgatagaga
     1921 aataattctt aaggggaata ttaaagggct gaacaaactt tatactagcg acaacatata
     1981 cgccatgaaa gatttgttgg ataggggcta caagggtaaa atcgatttaa tttatataga
     2041 tccccctttt tttaccatgg ccaattattg gcatagaatt tcacttggtg aaaaaaatat
     2101 aaggtattta gcctataatg atatctggaa agatggatta ttagaatact tggaaatgtt
     2161 aactgtaagg ttatatttga tgaaagaatt attatcatcc agaggtacta tttatgtcca
     2221 tctagattat aggacagttc actatgtaaa aattataatg gattgtattt ttgggagaga
     2281 aagattttta aatgaaatta tctgggccta taggtctggt ggagccagta gaaggcattt
     2341 tgctagaaaa catgatacta ttcttgcata tacaaagact gaagaataca tatttaatcc
     2401 ccagaaagaa aagtcttata atagaggctt gaaaccttat aggtttaaag gggtaaaaga
     2461 ataccaagat gaa
//
LOCUS       DATGSI010000250         1956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_538362, whole genome shotgun sequence.
ACCESSION   DATGSI010000250 DATGSI010000000
VERSION     DATGSI010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538362"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="murI"
                     /locus_tag="VK031_03820"
     CDS             complement(<1..663)
                     /gene="murI"
                     /locus_tag="VK031_03820"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HLS53075.1"
                     /translation="MIDTRPIGVLDSGVGGLTVAKELKKLLPGENIIYFGDNANVPYG
                     NKSEKEIYRLTKKMVDFLIEKDAKMIAIACNTISSLLDKYFKDYQVPIVSIIKPAANY
                     VVKNNITKVGVIATKFTIESGAYQNLIKAGNKGTKVVAEPSPDLAAVVDSANFTDKQV
                     DQIVDLHISNMFKEEELSHIILGCTHYPIVLENFQKRAPKTEFINPAYEQVLFMKDLM
                     DKE"
     gene            complement(769..>1956)
                     /locus_tag="VK031_03825"
     CDS             complement(769..>1956)
                     /locus_tag="VK031_03825"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HLS53076.1"
                     /translation="LIPYFIFVILIGFTGVIGEMAFGRAMEAGPLGAFKKALERRGKK
                     HGDLIGLIPVIGSLGIAIGYSVVVGWILRFTVGALSGSLTGAADSGAYFGAIAGKFGS
                     LGWHMAALVLTFTVMVAGISAGIEKINKFMMPAFFSMFLILAVRVFFLPGAEIGYSYL
                     FKPEWAALRDPKTWVFALGQAFFSLSLAGSGTLVYGSYLKKTEDIVNSALMVSIFDTM
                     AAMLAALVIIPSVFAYNLNPAAGPPLMFITMPEIFKQMPAGRLFASIFFIAVLFAGLT
                     SLVNLFEAPIESLQDRFKFSRAKAVATVGIIGAAVGIFIEDGDVLGAWMDVVSIYVIP
                     LGAMLAGIMFYWVCGPKFAREQVQLGRDKELGPWFEPMSRYVFVGLTIIVYILGIFYG
                     GIG"
BASE COUNT          588 a          473 c          301 g          594 t
ORIGIN      
        1 ttccttgtcc atcaaatctt tcataaaaag aacctgctca taggctggat ttataaattc
       61 tgtctttgga gctctttttt ggaagttttc taaaactatg ggatagtggg tgcatcctaa
      121 gataatatga cttaattctt cttctttaaa catattgctt atatgtagat ccacaatctg
      181 gtccacctgt ttatcagtaa agttggcact atctaccact gccgccagat ctggacttgg
      241 ctctgccacc acctttgtac ccttattacc tgcctttata agattttgat aagccccaga
      301 ttctatggta aactttgtgg ctataactcc taccttggta atattatttt taaccacata
      361 atttgcagct ggcttaataa tacttactat aggcacttga tagtccttaa agtacttatc
      421 taaaagtgag gatatggtgt tgcaggcaat ggcaatcatc tttgcatcct tttctattaa
      481 gaaatctacc atttttttag ttaacctata aatttctttt tcacttttat tgccataggg
      541 tacattggca ttgtcaccaa agtaaataat attttcccca ggcaataact tttttaactc
      601 cttggctaca gtaagacctc ctacccccga atccaaaact cctataggcc ttgtatctat
      661 catatatatt accccctttt ctttttctta cctccatttt aatacaagtt aagatcttat
      721 gcaattatat aaaaaggcta ctgggctctt tcccagtagc catattttct atcctatacc
      781 accatagaaa attcctagta tataaactat gattgtaagc cctacaaaca catatcggct
      841 cattggttca aaccaaggac ccagctcctt gtccctacct agttgtacct gctcccgagc
      901 aaactttggt ccacataccc aataaaacat aattcctgcc aacatggctc ccaatggaat
      961 cacatatata ctgaccacat ccatccaagc tcctaagacg tccccatcct caataaagat
     1021 acctacagct gccccgatta taccaactgt agccactgcc ttagccctgg agaatttaaa
     1081 cctatcctga agagactcta ttggggcctc aaataagtta accaaggatg ttaagccagc
     1141 gaagagaacc gctataaaaa atattgaggc aaataacctg cctgctggca tctgtttaaa
     1201 gatctccggc atggttataa acattaaggg tggtccagct gctgggttta ggttgtaggc
     1261 aaatactgat ggaattatta ccaaggctgc taacatagct gccattgtat caaatatgga
     1321 taccataaga gctgaattta ctatatcctc tgtctttttt aggtaactac catacactaa
     1381 agtaccagaa cctgccaagg atagggagaa aaatgcttgc cccagagcaa atacccaggt
     1441 tttcggatct cttaatgctg cccattctgg cttaaataaa tagctgtagc ctatctctgc
     1501 tcctggtaga aaaaacaccc ttacggccaa aattaaaaac atactaaaaa aggcaggcat
     1561 cataaattta tttatctttt caatacctgc agatattcca gctaccataa ctgtaaaggt
     1621 aaggaccagg gctgccatat gccagcctag actaccaaat ttacctgcta tggctccaaa
     1681 ataagccccg ctgtctgctg cacctgttag gctaccagat aaggctccca cagtaaatct
     1741 tagtatccag cctactacca ctgaatatcc aatagctatg cctaaagatc ctataaccgg
     1801 tataagtcca attaaatctc catgtttttt cccccgcctt tctaaggctt tcttgaaggc
     1861 tcccaagggt ccagcctcca tggccctacc aaaggccatt tccccaatta cccctgtaaa
     1921 tcctatcaat ataacaaata taaagtaagg tattaa
//
LOCUS       DATGSI010000251         1332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_64264, whole genome shotgun sequence.
ACCESSION   DATGSI010000251 DATGSI010000000
VERSION     DATGSI010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64264"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VK031_03830"
     CDS             <1..206
                     /locus_tag="VK031_03830"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HLS53077.1"
                     /translation="EDNENLKVVYDPEHPDADEDGYVFMPNVNLVDEMIALLNTQRTY
                     EANVTALNTSKSILKKALEISKD"
     gene            242..571
                     /gene="fliE"
                     /locus_tag="VK031_03835"
     CDS             242..571
                     /gene="fliE"
                     /locus_tag="VK031_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HLS53078.1"
                     /translation="MNILSLYNNNFLKTNQAIKNQTSQLGPVNRQAKEEGETSFKDLV
                     GRELERLNNQQIKADEMIKDFISGQADDLHTVLIATEEARLTLELAVQIRNKCIEAYR
                     EINNMQL"
     gene            597..>1332
                     /gene="fliF"
                     /locus_tag="VK031_03840"
     CDS             597..>1332
                     /gene="fliF"
                     /locus_tag="VK031_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HLS53079.1"
                     /translation="METFSKIVEGTKSGWNSMDRKKKIIMVGLVVGIILIALLLSYFS
                     RQVTYATLFTNLEPEDAGTIVEDLKSKNMKYKLENGGRDILIDEKHVDSYRLQLAMEG
                     NMPEKSSGFEIFDNMGLMVTDEDRKIMYQRALAGELQRSIMSLDAVNYAKVHLVMSEK
                     SIFETEEKEASASVFLDLNPGKKITDNMIRGIAALVSGAVDNLPEENIKIIDSKGNLL
                     SNILEEGDDLGAINALDQYQKARESFQ"
BASE COUNT          502 a          215 c          282 g          333 t
ORIGIN      
        1 cagaagataa tgaaaactta aaggtagtat acgatccaga acatccagat gctgatgagg
       61 atggttatgt atttatgcca aatgtaaacc tggtagatga aatgattgcc cttttaaata
      121 cccaaagaac ctatgaggca aatgtaactg cactaaacac cagcaaaagc atactaaaaa
      181 aggccttaga aatatctaag gattaaaata tggaatttaa tcctaggaaa tgagggataa
      241 aatgaatatt ctatcacttt ataataataa ctttttaaaa actaaccaag ccataaagaa
      301 tcaaacttct cagctgggcc ctgtaaatag gcaagccaaa gaagaagggg aaacatcctt
      361 taaggacctg gtaggcaggg aattggaaag gctaaacaac caacaaatta aggcagatga
      421 gatgatcaaa gactttataa gtggacaagc tgatgaccta catactgtcc tcatagccac
      481 agaggaagcc aggttgaccc tagaactagc tgtccaaata aggaataaat gcatcgaagc
      541 ctatagagaa atcaacaata tgcagttata gtaattaaaa ggagttatgc tatattatgg
      601 aaactttttc aaaaatcgta gaaggaacaa aaagtggctg gaatagcatg gacagaaaaa
      661 agaaaataat tatggtgggc ctggttgtag gtattatact aattgccctt ttactttcct
      721 actttagcag acaggtgact tatgcaacct tgtttaccaa cttggagcct gaagatgcag
      781 gcactattgt tgaggatttg aaatctaaaa atatgaaata caagctggaa aacggtggcc
      841 gagatatttt aattgacgaa aagcatgtgg acagctacag gctacaattg gccatggaag
      901 gtaatatgcc tgaaaaatcc tctgggtttg aaatctttga caatatgggt ttaatggtaa
      961 cagacgaaga cagaaagatt atgtatcagc gggctctcgc aggagaactt caaagatcca
     1021 tcatgtcttt agatgcagta aattatgcca aagtacactt ggttatgtct gaaaaaagta
     1081 tatttgaaac tgaagaaaag gaggcctctg cttctgtctt tttagatcta aatccaggca
     1141 aaaagataac agacaatatg ataaggggaa tagcagccct agtttctgga gcagtagaca
     1201 atctaccaga agaaaatatt aagattatag actctaaggg taacctttta agtaatatct
     1261 tggaagaagg agatgaccta ggggctataa atgctttgga ccaataccaa aaggcccggg
     1321 aaagcttcca gg
//
LOCUS       DATGSI010000252         4450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_490535, whole genome shotgun sequence.
ACCESSION   DATGSI010000252 DATGSI010000000
VERSION     DATGSI010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490535"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1404
                     /locus_tag="VK031_03845"
     CDS             28..1404
                     /locus_tag="VK031_03845"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HLS53080.1"
                     /translation="MRKEIYNMTQKEINRLRVINQTIDKVETISGAAEALDLSERQVS
                     RLKKGVMEEGPSFIIHGNRGRKPKHATANETKDLIIKLKKTKYKEANFLHFQELLEEH
                     ENIKVSYPTVYRILTEAGIKSPKKHRKRKTYHRRKRKPQRGMLVQIDASPHTWIIGEK
                     AFDLHGAIDDATGEILALHFTPNECMEGYFEIVRQIVSNHGIPTSFYCDRHTIFISPN
                     DGKLSIEDQLKGKQVNLTQFGRAMDELGINIIKAYSPQAKGRIERLWGTLQSRLPVEF
                     KIHGITTIEDANAFLVKFIEKYNKKFSVEPEDFKSAFRDLDSNINLDHILCIKDERTI
                     IEGSAFSYGGQYYQLIQNGKKVASMPKAKLTVLTSSRIGIKATYSGVVYDTKTLDERP
                     KKAVTNQSKSKKSQNNKTKPAKDHPWRQPINKKPIQSYGDDLLYNEYDAKIAEILKDL
                     FNSTRAWA"
     gene            complement(1754..2413)
                     /locus_tag="VK031_03850"
     CDS             complement(1754..2413)
                     /locus_tag="VK031_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLS53081.1"
                     /translation="MSRENIKLLIIEDQLLIMEGLCKICEETPDIKVVGQANNGKYAI
                     DLLKEKNPDVILLDMDMPVINGLETLRMIKKQGAKGKVIMLSDHNSKNHVIEAMEIGA
                     KGYISKNASSKELIKTIRYVHSGNTYIQPNIKNDSQGADDNKKEDEVSDKIKQLSKRE
                     YEILYLLASGYKNKEIGDRLFISEKTVKNHITSIYKKLGVEGRVQAVIFAYTHQIKTL
                     S"
     gene            complement(2430..3542)
                     /locus_tag="VK031_03855"
     CDS             complement(2430..3542)
                     /locus_tag="VK031_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HLS53082.1"
                     /translation="MTYEFLRAFMLVFIAELGDKSQIIAMTFATKYVIKDVIWGIFFG
                     IFLNHGIAILLGKYLAAVVPDHIVALVAGLMFVFFAYTSLENEEVEKLEERERLNPVI
                     TVAIAFFIGELGDKTQLTAITLATESNYPLLILMGTILGMLVSSGLGILVGMKIGDQI
                     SELSIKISSSLMFLTFGCIKLYGVTGNYKRIYVYLIFVSLLLIELVLILDFVQKYRKR
                     GKGSALKQAASNLYQQTAELKLALDSICIGENKCGQCRGADCLVGYIRHCLSNAREKD
                     QYYKEYYLDVDRLIKKGYDKEKVMQALELIIQDYQEYGWVDHSRFVCNRIRTVLEYIL
                     LEQAIEGITNVDTYIDNVKKINHHLGKRLENRVKTY"
     gene            complement(3612..>4450)
                     /locus_tag="VK031_03860"
     CDS             complement(3612..>4450)
                     /locus_tag="VK031_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HLS53083.1"
                     /translation="GIIDAYDKKIGQVRKHEGQVNTAANLRLLLKGSNMITRQGEARV
                     QDAYSLRCVPQVHGASKDTFKYVQEKIEIEMNSATDNPLIFTDSGEVISGGNFHGQPL
                     ALPFDFLGIAVAELANISERRIERLVNPTLNNGLPAFLIGNAGLNSGFMITQYVAASL
                     VSENKVLSHPASVDSIPSSANQEDHVSMGTIAARKAAEIIDNAYKVIAIEAIAACQAI
                     DLRAGKELGLGTQVAYSMVREGIKTVKEDKIMYVDICDCEEMLRKGGLVGKVEKIVGQ
                     LW"
BASE COUNT         1420 a          880 c          662 g         1488 t
ORIGIN      
        1 ttaaaattat tctgataaaa tgtcagtatg agaaaggaaa tatataatat gactcaaaaa
       61 gaaattaaca gacttagagt tatcaatcaa acaattgata aggtagaaac tattagtgga
      121 gctgctgaag ctttagatct aagtgaacga caagtttcaa ggttaaagaa aggagttatg
      181 gaagaaggac cttcgttcat tatccacgga aatagaggtc gtaagcccaa gcatgctaca
      241 gctaatgaaa ctaaagactt aattattaag cttaaaaaga caaaatataa ggaggccaat
      301 tttctacact ttcaggaatt attagaagag catgaaaaca taaaggttag ttatcccact
      361 gtgtatagaa tcctaactga agctggtata aagagcccta aaaaacatcg taagcgaaag
      421 acttatcacc gtagaaaaag aaaaccgcaa agaggcatgt tagtgcaaat tgatgcttca
      481 ccacatactt ggattattgg cgagaaagct tttgatcttc atggtgctat cgatgatgct
      541 acaggtgaaa ttcttgccct tcattttacc cccaatgagt gcatggaggg gtattttgaa
      601 atcgtcaggc aaattgtttc caatcacggt attccaacca gtttttactg cgatcgccat
      661 accattttca tttcccccaa tgatggtaaa ttatctattg aagaccaatt aaaagggaag
      721 caagtaaatt taacccaatt tggtagggct atggatgagt tagggattaa tattatcaaa
      781 gcatattcgc cacaggccaa gggccgaatt gaaaggctat ggggtacctt acaaagtaga
      841 ctacctgttg aattcaaaat tcacggaatt accaccatag aggatgcaaa tgctttctta
      901 gttaagttca tagaaaagta taataaaaag tttagtgttg aacctgagga ttttaaatct
      961 gcctttagag atctagattc aaatattaat cttgaccata tactttgtat aaaggatgaa
     1021 cgtacaatta ttgagggttc agcattttca tatggtgggc agtattatca gcttattcaa
     1081 aacggaaaga aggttgctag tatgccaaag gctaaactta ccgttttaac tagctctaga
     1141 attggtataa aggctactta ttctggtgta gtttacgata ccaagacatt agatgagcgt
     1201 cctaaaaaag cagttactaa tcaatctaag agtaagaaat cacaaaacaa caaaaccaag
     1261 ccagctaaag atcatccttg gagacaacca ataaataaaa agcccataca atcgtatgga
     1321 gatgatcttt tatataacga gtacgatgcg aaaatcgctg aaatattaaa agacttgttc
     1381 aactccacaa gagcatgggc ttaagagagg ttaaacctct gtaaatataa tatctttttt
     1441 ccagtccttt ttcaagggct tatttcctat acccttttat cgagatattg taacgtttat
     1501 gcctcgaaag cctattgatg tgggagaatg ccccatgtca ataggatcat agaataagtt
     1561 cggtgtttaa tcttattatt ttttatttta aagatgacat tttcattgaa taaaattaga
     1621 tatttttaca tgacattttc actgaatatt gacataactt ttttcatcat tagaacaatt
     1681 tataaaaact tctctagggc tacttattat atattgattc ttgtaaaaat atacatctta
     1741 ccactagctc tttctaactt aatgttttaa tctgatgggt atatgcaaaa attactgctt
     1801 gtaccctacc ctctactccc aactttttat atatactagt aatatggttt ttgacagttt
     1861 tctcacttat aaacaatcta tctcctatct ccttattttt atatcctgat gctaacaggt
     1921 aaagtatttc atattctctt tttgatagtt gtttaatttt atccgaaact tcatcttctt
     1981 ttttgttatc atcagccccc tgggagtcat tttttatatt tggttgtata taggtattgc
     2041 cagagtgaac atatcttatt gtttttataa gttcttttga actggcattt ttgctaatat
     2101 atcccttggc cccaatttcc atagcctcaa taacatgatt tttactatta tgatcagaaa
     2161 gcatgattac ttttcctttt gccccttgtt tttttatcat tctaagagtt tccaaaccat
     2221 taattacagg catgtccata tctaaaagta ttacatctgg atttttttct ttaagcaagt
     2281 caatagcata tttcccatta ttggcctggc ctacaacctt aatgtccgga gtttcttcac
     2341 atattttaca taaaccctcc ataattaata attggtcctc tataattaat aattttatgt
     2401 tttctctaga catagttcca cccctaacct tagtaagtct ttaccctatt ttccaatctc
     2461 tttcccaagt gatgatttat ttttttaaca ttatcaatat atgtatcaac attagtgatc
     2521 ccttcaatag cttgttcaag taaaatatat tccaaaactg tcctaattct attacagaca
     2581 aatcgagagt ggtctaccca tccatactct tgatagtctt ggataattag ctccagagcc
     2641 tgcatcacct tttctttatc atatcctttc ttaattaatc tatctacatc taaataatat
     2701 tctttatagt actgatcctt ttccctagca ttacttaaac aatgtcttat atatcctact
     2761 aaacaatcag cccccctaca ttggccacac ttgttttcac ctatacaaat actatctagt
     2821 gccaacttta actctgctgt ttgctgatac aggttggatg ctgcctgttt taaggcagag
     2881 ccttttcctc tttttctgta tttttgaaca aaatccaata ttaatactag ctctattaaa
     2941 agtaaagaga caaaaattaa atagacataa atccttttat aatttcctgt tacaccatat
     3001 aacttaatac aaccaaacgt tagaaacata agcgatgagg aaatttttat agacaattca
     3061 gagatctgat ctccgatttt catgcctacc agtataccca agccactact aactaacata
     3121 cctaatatgg tacccatcaa tattaataag ggatagttgc tttcggtggc cagagttatg
     3181 gcagttaact gagtcttgtc tcccaattcc cctataaaaa aggctatggc cacagttata
     3241 acaggattta accgttccct ttcctctagt ttttcaactt cctcattctc cagggaggta
     3301 taagcaaaga agacaaacat taggcctgcc actaaagcca caatatgatc aggtaccact
     3361 gctgccaaat acttgcccag taatatggct atgccatgat ttaaaaaaat accaaaaaat
     3421 attccccaaa ttacatcctt aatcacatac ttggttgcaa aggtcatagc tataatctgg
     3481 gatttgtctc ctaattcagc tataaatact aacataaatg ctcttaaaaa ttcataagtc
     3541 ataaaagatc tacctcttat ttaaatgttt aattcctgtc tatgatgtca agaatataac
     3601 catttttaaa gctaccacaa ttgtcctact attttttcaa cctttcctac aagccctccc
     3661 ttcctaagca tctcttcaca atcacatata tcaacataca tgattttatc ctcttttaca
     3721 gtttttattc cttccctaac catactatat gccacttgtg tgcctaaccc taactccttg
     3781 cctgccctta aatcaatagc ttggcaagct gctatggctt ctatagctat aaccttatag
     3841 gcattatcta ttatctcagc tgccttcctg gctgcaatgg tccccataga cacatgatct
     3901 tcctggttag cagaagaagg tattgaatca acactggcag gatgtgacaa aaccttattt
     3961 tcagatacta aggatgctgc aacatattgt gtaatcataa agcctgaatt caggccagca
     4021 tttcctatta agaaggctgg taaaccattg tttaatgtgg gatttaccaa tctttcaatt
     4081 ctcctttcag agatattagc caactcggcc acggcaatac ctaaaaaatc aaaaggtagg
     4141 gctaaaggtt gaccatggaa atttcctcct gaaatgacct ctccactatc tgtaaagata
     4201 agtggattgt ctgtagccga gttcatttca atttctattt tttcctggac atatttaaaa
     4261 gtatctttac tggccccatg gacctgagga acacacctta aggaataggc atcctgtact
     4321 ctagcctccc cttgcctggt tatcatatta cttcccttaa gcagtagtct aaggtttgct
     4381 gctgtattta cctgtccttc atgctttcta acttgcccaa tttttttatc atatgcatca
     4441 attataccat
//
LOCUS       DATGSI010000253         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_632811, whole genome shotgun sequence.
ACCESSION   DATGSI010000253 DATGSI010000000
VERSION     DATGSI010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632811"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VK031_03865"
     CDS             complement(<1..328)
                     /locus_tag="VK031_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53084.1"
                     /translation="MNRPFVLLLFPLTFGILFNHFFQISLFTVLFTLILVLVYIFYNI
                     IKGHLNQSIFILLIFILGILVSIEKDKSLMEEYIDTSPNIVACVDQVILQSDNNSKYV
                     IQVKEVD"
     gene            441..>1161
                     /locus_tag="VK031_03870"
     CDS             441..>1161
                     /locus_tag="VK031_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HLS53085.1"
                     /translation="MSLLTQKIFLDNSYAKEVNAEIISKEFKNNKYLIKLNRTIFYPH
                     LAGGQPRDRGTIEGIQVLDVYEEGDDIIHVVNKDINKRYVQLKIQWDHRFDLMQQHTG
                     QHLLSSSFYRLFNVRTLGFHIGRDYIYIDIEKSNLLEDEVRHVEYLSNKIIHSNFPIK
                     TYFINQDRVDSIPLRGDVPKVENIRIVEIDNIDYAACCGTHLSSTGEIGLIKIRRWER
                     YKGNTRVEFVCGNRALDDYNWK"
BASE COUNT          430 a          162 c          198 g          371 t
ORIGIN      
        1 catctacttc tttaacctgt ataacatatt ttgaattatt gtctgactgt aaaataacct
       61 ggtctacaca ggccacaata ttcgggcttg tgtctatgta ctcctccatt aaacttttat
      121 ccttttcaat acttacaagg attcctaata taaagattaa aaggatgaaa atgctttgat
      181 ttaaatggcc ttttattata ttataaaata tgtatactag caccagtatt aatgtaaata
      241 ggactgtaaa taaactaatt tggaagaaat gattaaacaa tataccaaat gttagtggaa
      301 ataataacaa aacaaatggt ctattcataa tccactccta aagtagctta tttatattaa
      361 tatataaatt ataacatatt gaaagcatga aaatatttaa tatttttaat agatatcttt
      421 attaatttgg gaaaggttcg gtgagcttgt tgacacagaa gatatttttg gacaactctt
      481 atgcaaagga agtcaatgca gaaattatta gcaaggaatt taaaaataac aaatacctta
      541 taaaattgaa taggaccata ttttatcccc atcttgcagg tggacaacct agagacagag
      601 gtactattga aggaatccaa gttctagatg tttatgaaga aggagatgac ataatccatg
      661 tggtcaacaa agatataaat aaaagatatg tccagcttaa gatccaatgg gaccataggt
      721 ttgatttaat gcaacaacat acaggccagc accttttgtc atcttccttt tataggttgt
      781 ttaatgtaag aaccctaggc ttccatatag gaagagacta tatttatata gatattgaaa
      841 aatcaaacct tctagaagat gaggtaagac atgtagaata tttatctaat aaaataatcc
      901 actctaactt tccaataaaa acttatttta tcaaccagga tagggtagat tctatacctt
      961 taagaggtga tgttcccaag gttgaaaata tacgtatagt tgaaatagat aatattgact
     1021 atgctgcctg ctgtggcacc catcttagtt caactgggga gataggatta attaagatta
     1081 gaaggtggga aaggtacaag ggcaatacta gggtagaatt tgtatgcggt aatagggctc
     1141 ttgatgatta taattggaag a
//
LOCUS       DATGSI010000254         1442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_538465, whole genome shotgun sequence.
ACCESSION   DATGSI010000254 DATGSI010000000
VERSION     DATGSI010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538465"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VK031_03875"
     CDS             <1..194
                     /locus_tag="VK031_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53086.1"
                     /translation="TKLNVWQWMIAIVSYITIINLIIKIIFGLTWLYVFSAVIAVGTL
                     SLITFIISFAIILYKSWRK"
     gene            182..670
                     /locus_tag="VK031_03880"
     CDS             182..670
                     /locus_tag="VK031_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53087.1"
                     /translation="MEEVISEEEIRQITLEVINKFVIPDFEERGHNASGNWIESIDVR
                     AEHNKGIITGPAYTGALTDGRGPNKNQDPEAIANWAWWYGGNVFKPWAESKGLDLNPY
                     AVAYTIAKRGTKIYREGGSDFLEILETPEVKDYIIQRISFMATANVKLMLKESLQKLK
                     NS"
     gene            736..>1442
                     /locus_tag="VK031_03885"
     CDS             736..>1442
                     /locus_tag="VK031_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53088.1"
                     /translation="MNYRLWINLNVQGEQYIFSMTPMAGVVKFDLAKIVLGLVGNVSN
                     KTNLVIKHSPVSSKWSVDGAYNVSIYFTDTNIYSVSSRGMIINKTFVIGGVSEYKSNV
                     PVGNTLSINNNKWDGYPTFDFKLNQGNIIGTSVSQVNRRKRIKCNDAYILFRNRLGGF
                     SGYLFEDFEITENARNKGYYITDNNIVDQGTTLEKSISLQTKLKRRDYEVARHLIDSR
                     EVYLYRDINKLTRLVGG"
BASE COUNT          534 a          188 c          301 g          419 t
ORIGIN      
        1 gcactaaatt aaacgtatgg caatggatga ttgcaatagt aagctacatt actataatta
       61 atcttataat taagataatc tttgggttaa cttggttgta tgtgttttcg gctgttattg
      121 ctgtaggcac actatcactg ataactttta ttatatcctt tgctataatc ctatacaaat
      181 catggaggaa gtaatatcag aagaggaaat aagacagata acattagagg ttattaataa
      241 atttgttatt ccagattttg aagagcgagg gcataacgca tcaggcaatt ggattgagtc
      301 gatagatgta agagctgagc ataacaaagg gattattact ggacctgctt atacaggtgc
      361 gttaaccgat ggtagaggac ccaacaaaaa ccaagaccca gaagccattg cgaattgggc
      421 atggtggtat ggtggaaatg tattcaagcc atgggcagaa agtaagggat tagatttaaa
      481 cccttatgca gtagcttata caattgcgaa aagaggaaca aagatatatc gagagggggg
      541 cagtgatttc cttgaaatat tagaaacgcc ggaagtaaag gattatatta tacaaagaat
      601 tagctttatg gctacagcta acgtaaaact catgcttaaa gaaagcttac aaaaactaaa
      661 gaactcatga taataaaagg attagagcat aatgcgtatt acttgtataa tcctatatgg
      721 gtagaggtaa gtgatttgaa ttatagactt tggataaact taaatgtaca aggagaacaa
      781 tacatattct ctatgacacc gatggcaggt gtagttaagt ttgacttagc taagatagtg
      841 ttaggattgg taggtaatgt aagtaataag acaaatttgg ttataaagca ttccccagta
      901 tcatccaaat ggtcagtgga tggagcttac aatgtatcta tatactttac agacactaac
      961 atatattcgg taagttcaag gggtatgata ataaataaaa cttttgtaat tggtggggtt
     1021 tctgaataca aaagcaatgt accagtaggc aacacgttat caataaataa taataaatgg
     1081 gatggctatc caacgtttga ctttaagctt aatcagggga atattatagg cacatctgta
     1141 agtcaagtca ataggcgtaa acgaatcaaa tgtaacgatg cctacatatt atttagaaat
     1201 aggctaggtg gcttttcagg ttatttattt gaagattttg aaataacaga aaacgctagg
     1261 aataaagggt actatataac agataataat atagtagatc aaggcactac actcgaaaag
     1321 tccatttcat tacaaacaaa attaaaacga agagattatg aagttgcaag acatttaatt
     1381 gactctaggg aagtgtattt atatagagat ataaataagt taacaaggtt agttggtggt
     1441 aa
//
LOCUS       DATGSI010000255         1911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_679564, whole genome shotgun sequence.
ACCESSION   DATGSI010000255 DATGSI010000000
VERSION     DATGSI010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679564"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VK031_03890"
     CDS             complement(<1..1041)
                     /locus_tag="VK031_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLS53089.1"
                     /translation="MHILIYFDNAATSFPKPNIVYEQTMEAMKEYGANPGRSGHKLAL
                     ESNRAIFDARDLLAQFFNANKATEIVFTSNCTESLNLAIKGLLKPKDHVITTSMEHNS
                     VIRPLKRLEEIGVKTTIVRANREGLIDPADIESAINENTKLIVTTHISNLTGSILPIE
                     EIGHIARKHNIYYLVDAAQSAGVYKIDVEKMNIDMLAFPGHKGLLGPQGTGGLYIREG
                     LDLKEMLQGGTGSSSESLVQPSLRPDKYESGTQNGSGIVGLGAGIRFINKKGIDKIRI
                     HEEELTEHFIKEAKKIGRVKLYGPLDIKKQGAVVALNIESMDSSELSYILDERYNIAV
                     RPGLHCTPLGHET"
     gene            complement(1207..1845)
                     /locus_tag="VK031_03895"
     CDS             complement(1207..1845)
                     /locus_tag="VK031_03895"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HLS53090.1"
                     /translation="MLIKKSLQDYISQTASGSPTPGGGSVSALAASLGSALTNMVGNL
                     TFGKKDYEALDQEVKEKMKRNFDKLEEKTKILQAIVDEDSKAFSQVIKAFQLPNHSEE
                     EKRLRSQAIQAGYKKALEVPLKCAEECYQVLKLQEIFAQYGNTNAITDVGVGALLAYS
                     GLEGSLLNVRINLSSIRDEKYKKDMEEKINILLQNGKRLKETISKQVYDKLK"
BASE COUNT          517 a          402 c          270 g          722 t
ORIGIN      
        1 agtttcatgg cctaaaggtg tacagtgtaa ccctggtcta actgcaatat tatacctttc
       61 atctaatata tatgatagtt ctgatgagtc catactttct atatttaaag ctacaactgc
      121 cccttgtttt tttatatcta gggggccgta taattttacc ctcccgattt ttttagcctc
      181 cttgataaaa tgttccgtta attcttcctc atgaatccgt atcttatcta tacctttttt
      241 attaataaat cttattcctg ccccaagtcc tacaatacca gatccatttt gagtacctga
      301 ttcatactta tctggtctta gactaggttg gaccaaggac tccgaagagc ttcctgtacc
      361 tccctgtagc atttccttaa gatccagccc ctccctgatg tatagacctc ctgttccctg
      421 tggcccaagt aggcccttgt gtccaggaaa ggccaacata tcaatattca ttttttctac
      481 gtctatttta taaactcctg ctgattgggc agcatctacc aaataatata tattatgttt
      541 tcttgctata tgccctattt cttcaatagg taaaatactg cctgttaaat tagatatatg
      601 agttgtaact ataagttttg tattttcatt tatagcagat tctatatctg caggatctat
      661 taaaccttcc ctattagccc taactattgt cgtctttaca cctatttctt ctagcctttt
      721 taaaggccta attacagagt tgtgttccat ggatgtggta attacatgat ccttaggctt
      781 aagcaaacct tttatagcaa gatttaagct ttctgtacaa ttagatgtaa atactatttc
      841 cgtagcctta tttgcattaa aaaattgggc caaaagatct ctagcatcaa atatggccct
      901 attagattca agtgccagtt tatggccaga cctgcctgga tttgcaccgt attccttcat
      961 ggcctccata gtctgttcat atactatatt tggcttagga aaggaagtag ctgcattatc
     1021 aaaatatatc aaaatatgca cctccacttt aaacattata acatattctt tgccttttct
     1081 ttatatattt atcttatttt tgacttgcta ttttgttaat ctttaaacaa gaaaattaat
     1141 atatttttta ctatacagta tataaaaata aaactaggct tctttaaaag cctagtttag
     1201 tccttcctac tttaatttat cgtatacctg ttttgaaatt gtttctttta gtctttttcc
     1261 attttgtaaa agtatgttaa ttttctcttc catatccttt ttatattttt catccctaat
     1321 acttgaaaga ttaatcctaa catttaatag agaaccttcc aagccactat aggccaataa
     1381 ggcacctact cccacatctg ttatggcatt agtatttcca tattgagcaa atatttcctg
     1441 tagctttaat acctggtaac attcttctgc acattttaag ggaacttcta gagccttctt
     1501 ataaccagcc tggatagcct ggcttctcaa tcttttttcc tcttcactat gattaggtaa
     1561 ttgaaaggcc ttaattactt ggctgaaggc ttttgaatct tcgtctacaa tagcctgtaa
     1621 gatcttggtc ttttcttcaa gtttgtcaaa gtttcttttc atcttttctt ttacttcttg
     1681 gtctaaagcc tcataatcct ttttcccaaa ggttagattt cccaccatgt tggtaagggc
     1741 agatcctaaa ctggctgcta aagcagatac actaccacct ccaggtgtgg gactaccgct
     1801 ggccgtctgg ctaatatagt cctgtaaact cttcttaatt agcattggct tccctcctgt
     1861 ttattaaatg gacaaaattc catataaaaa tgttccacgt ggaacatttt t
//
LOCUS       DATGSI010000256         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_299351, whole genome shotgun sequence.
ACCESSION   DATGSI010000256 DATGSI010000000
VERSION     DATGSI010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299351"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1002)
                     /locus_tag="VK031_03900"
     CDS             complement(34..1002)
                     /locus_tag="VK031_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HLS53091.1"
                     /translation="MRKILVTGALGQIGSELTLKLREIYGDKNVVATARRKKEGHEEL
                     IETGPFEILDINDGNRALDIVRKYRINTIINLAAILSAVGEKDPLTCWDINMNGLINI
                     LEIGRSENCQIFTPSSIAVFGEDTPADNTPQTTIQRPSTIYGVTKVAGELICDYYYKK
                     YKVDTRGVRFPGLISYKTLPGGGTTDYAVHIYYDAIKYERYTSFIDKGTKMDMMYMPD
                     ALDAIINLMEADGDRLLHRNAYNVTAMSLDPESIGQSIKKIIPNFQLDYKVDPLRQQI
                     AESWPNSLDDSAARDEWGWKPKYDLDSMTKDMLEKLSEKIGIKSLG"
     gene            complement(1173..1997)
                     /locus_tag="VK031_03905"
     CDS             complement(1173..1997)
                     /locus_tag="VK031_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HLS53092.1"
                     /translation="MSKIKIVTDSTSYISKDYIEKEDVAVVPLNYVFDGETYLEGFKG
                     EFDDFFRKLENSNLFPTTSQPSVGDFHQVFKDALKEYDEIIAIVLSSKLSGTYNSAVL
                     AKDMLEDERVTIIDSETSASNLRFLVEDAVEMAKANKSSEEIIKHINNKKNSMKVFLT
                     AATLEYLKRGGRLSALQSTIGNLLNIKPIIELKDGQLQLLEKVRGKNRATSKIISYIH
                     ENVEKISICHILNLEEAEKFKSTLEEKFPKASITIDDLGPVVGSHLGPKAIGICFY"
     gene            complement(2065..>2718)
                     /locus_tag="VK031_03910"
     CDS             complement(2065..>2718)
                     /locus_tag="VK031_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLS53093.1"
                     /translation="GNVVNPDDIVNEFGADTLRSYEMFIGDFEKSAPWLESGVRGCRR
                     FLDRVWRLQEIVVEGNEYTKELETSIHKAIKKVSEDFETLKFNTGIATLMSLSNEFND
                     HGKITKKDFKTFITLLNPVAPHITEELWQIAGFDGYLHEGSWPEYDEEKIKEDTIEMP
                     VQVNGRVRGTIEVSLDDSQETVKEKALNDENIKRFVQDKTIIKEIFVKGKIYNIVVK"
BASE COUNT          796 a          552 c          344 g         1026 t
ORIGIN      
        1 atgtaaaaaa aagattagga aatcctaatc tttttaacct aaagacttta ttccgatttt
       61 ttcagataac ttttccaaca tatccttagt catagagtcc agatcatatt ttggcttcca
      121 accccattca tccctagcag cactatcgtc cagagaatta ggccaagact ctgcaatttg
      181 ctgtcttaaa ggatctactt tataatctag ttggaaattg gggattatct ttttgataga
      241 ctggcctatt gactccggat ccaggctcat ggctgtaaca ttataagcat ttctatgtaa
      301 taatctatcg ccatcagctt ccataagatt tataattgca tctaaagcgt ctggcatata
      361 catcatgtcc atttttgtac ccttgtctat aaaagatgtg tatctttcat atttaatagc
      421 atcgtagtag atatgtactg catagtcagt ggtgcctcca cctggtaaag ttttataaga
      481 gattaagcct gggaatctaa cccctcttgt atctacctta tattttttat aataataatc
      541 gcatattaac tcgccggcca cctttgttac accataaata gtgcttggcc tttgaatggt
      601 agtctgtggt gtattatctg ccggtgtatc ctctccaaat actgcaatgg aacttggggt
      661 aaatatttgg caattttcac ttctacctat ttctaaaata ttgattaaac cattcatatt
      721 tatatcccaa caggtaagtg gatccttttc tcccacagca gataaaatag cagctaggtt
      781 tataatagta tttatcctat attttcttac tatatctaag gctctatttc catcatttat
      841 atctagtatc tcaaagggac cagtttctat tagctcttca tggccttcct tttttcgcct
      901 agcagttgcc actacattct tatctccata tatttcccta agttttaagg ttagttcaga
      961 tcctatttgt cctaaggctc ctgttactaa aatttttctc actttcccac ctcctaagct
     1021 tgtagattct aggtattatt atatcatatt ttcccccata atctttaaca agtatttatt
     1081 ttccaaagag aaacaggcta ggattttctc cctagcctga atttaacagt actattatag
     1141 ctaatagctt ttaaaattat atccttgtta atttaataaa aacatatgcc tattgcctta
     1201 ggtcctaggt gagaacctac tacaggtcct agatcatcaa ttgttattga cgcctttgga
     1261 aacttttctt ccaaagtaga tttaaacttt tctgcctcct ctagatttag tatatggcaa
     1321 atactaattt tttctacatt ctcatgaata taagaaataa tttttgatgt tgccctattt
     1381 ttacctctta ccttttctaa cagttgtagt tgaccgtcct ttaattcaat aattggctta
     1441 atatttaaaa gattccctat ggtagattgt agggctgaca acctgccacc tctttttaaa
     1501 tactctaaag ttgccgcagt taaaaaaacc ttcatagaat ttttcttatt atttatgtgt
     1561 tttatgattt cttctgaact tttattggcc tttgccattt ctacagcatc ttcaactaaa
     1621 aacctaaggt tagatgcaga agtttctgag tcaataatag taactctttc atcctctaac
     1681 atatctttag caagtacagc gctgttatat gttccactta atttagaaga caaaacaata
     1741 gctattattt cgtcatactc ctttaatgca tctttaaata cttgatgaaa gtcacctact
     1801 gacggttgtg aggtggttgg aaataaatta gaattctcta atttacgaaa gaagtcatca
     1861 aactcacctt taaaaccctc caggtatgtt tctccatcaa atacatagtt taatggaact
     1921 actgccacat cttccttttc aatataatct tttgaaatat aactagtact atcggttaca
     1981 atctttatct ttgacattta aaaacctccc taaaaataat aatagtactg ttaaatttaa
     2041 cagtactatt atacatattt ataattattt aacaactata ttatatattt ttcctttaac
     2101 aaatatttct ttaataatag ttttatcttg aacaaacctt ttaatattct catcatttaa
     2161 tgctttttct tttacagttt cctggctatc atccaggctt acctcaatag ttccccttac
     2221 cctaccattt acctgtactg gcatttcaat agtatcttct tttatctttt cttcatcata
     2281 ctctggccaa ctaccttcat gtaaatagcc atcaaatcca gcaatttgcc ataattcttc
     2341 tgtaatatgt ggtgccactg gatttagtaa ggtgataaag gttttaaagt cttttttcgt
     2401 tattttcccg tgatcattaa attcgtttga taaagacatt aaagtggcta ttccagtatt
     2461 aaactttaaa gtctcaaagt cctcagatac ttttttaatg gccttatgga tgcttgtttc
     2521 caattcttta gtatattcat tgccttcaac cactatttcc tgtaatctcc ataccctatc
     2581 tagaaacctt ctacatcccc taactccact ttccaaccat ggagcagatt tttcaaagtc
     2641 acctataaac atttcataac ttcttaaggt gtctgcccca aattcattta ctatatcatc
     2701 tggattaaca acattacc
//
LOCUS       DATGSI010000257         1674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_161638, whole genome shotgun sequence.
ACCESSION   DATGSI010000257 DATGSI010000000
VERSION     DATGSI010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161638"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VK031_03915"
     CDS             complement(<1..113)
                     /locus_tag="VK031_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HLS53094.1"
                     /translation="MLTQFKVVKRLVLSALLGGVIGYEREINNRPAGLRTH"
     gene            complement(143..1639)
                     /locus_tag="VK031_03920"
     CDS             complement(143..1639)
                     /locus_tag="VK031_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HLS53095.1"
                     /translation="MENIIAGFINAFQFVNIIAALISVTIGIIVGSLPGLSAAMGVAL
                     LIPITFGMPAETALIALAGVYCGAMYGGSISAILLHTPGTSSAAATAIDGYPMTLKGE
                     AGKALATASVASFIGGIISSIALYTLSPLLASLALKFGPIEYFWLSIFGLTIIAGVSS
                     DSMLKGLISGALGLVLSTIGMDPMLGIPRFTFGNNALLEGLPFTATLIGLFSMSQVLL
                     LAEKKLKDMRKAKKVEDKVLLNKDELKRITPVSVWSAIIGTVVGILPGAGGTIASFIG
                     YNEAKRLSDNRDKFGTGVIEGVAGPEAANNAVTGGSLIPTLTLGIPGNSVTAILLGGL
                     VIQGLRPGPDLFTIHGNITYTFFAGFVIVNIFMLLLGLIGVRLFAQVSRINDSILIPL
                     IFTLSVVGSYSITNNFMDVWVMFIFGIIGYFVKKFNLNSAAIVLALILGPIGETGLRR
                     TLIMHQGDYTVLFSSVISWVLIGISILSLSTPFVMSYLEKKRLMKEEA"
BASE COUNT          536 a          359 c          243 g          536 t
ORIGIN      
        1 atatgggttc ttagaccagc tggtctattg tttatttctc tttcgtatcc gattactcct
       61 cccagtaatg cggataaaac aagtcttttc acaactttaa actgtgttaa catgataaaa
      121 cccctatttt tatcatttct tattaagctt cttctttcat aagtcttttc ttttctagat
      181 aagacattac aaatggcgtg gataaagata atatggaaat tcctatcaat acccaggata
      241 ttacgctact aaataaaact gtataatctc cttgatgcat aattaatgtt cttcttaatc
      301 cagtttcccc aataggtcct aggattaaag ctagaactat agctgctgag tttaaattaa
      361 actttttcac aaaatatcct attattccaa agataaacat gacccataca tccataaagt
      421 tattagtaat ggaatatgaa cctactactg atagggtaaa tatcaatgga attagaatag
      481 aatcatttat cctagaaact tgtgcgaaca atcttacacc aattaatccc agtaacaaca
      541 taaatatatt tactattacg aacccagcaa aaaatgtata ggttatatta ccatgaattg
      601 taaacaagtc tggaccaggc cttaatccct gaatcactag tccacctaac aaaatagctg
      661 taacgctgtt tcctggtata cctagtgtta aggtcggtat taatgaaccg ccagttacag
      721 cattgttagc agcttctggt cctgctacac cttcaattac tcccgttcca aatttatctc
      781 tattatcgga taatcgtttt gcctcattgt atccgataaa acttgctata gttcctcctg
      841 caccaggcaa tattcctact actgttccta taattgctga ccaaacactt acaggtgtaa
      901 tcctttttag ttcatcttta tttaataaaa ccttatcctc aaccttctta gcctttctca
      961 tatcctttag ctttttctct gccaatagta gaacctgtga catagaaaat aaacctatta
     1021 aagtagctgt aaatggtaat ccctctagca atgcattatt tccaaatgta aatcttggta
     1081 ttcccaacat aggatccatc cctatggtgg ataaaactaa accaagggcc cctgaaatta
     1141 gtccttttaa catcgagtct gagcttacac cagctataat ggttagccca aaaatagata
     1201 gccaaaaata ctctattggt ccaaatttta atgccaaaga tgctaataat ggcgaaaggg
     1261 tataaagggc aattgagcta attattccac ctataaagga tgctactgat gcggttgcta
     1321 aagctttccc cgcctctcct ttcaaggtca ttggataccc atcaatagca gttgctgccg
     1381 ctgatgatgt tcctggagta tgaagaagga ttgcagaaat cgacccccca tacattgcac
     1441 cacaatatac tcctgccaag gctatcaagg ctgtttctgc tggcatacca aaagttattg
     1501 gtatcaaaag ggcaactccc attgccgcag ataaaccagg taaggatcct actattatac
     1561 ctattgttac acttattaaa gctgcaatta tatttacaaa ttgaaaggca ttaataaagc
     1621 ctgctataat attttccata atttcacccc gcaattaaat tattaaccct tttg
//
LOCUS       DATGSI010000258         1203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_250367, whole genome shotgun sequence.
ACCESSION   DATGSI010000258 DATGSI010000000
VERSION     DATGSI010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250367"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1203
                     /locus_tag="VK031_03925"
     CDS             <1..>1203
                     /locus_tag="VK031_03925"
                     /inference="COORDINATES: protein motif:HMM:NF027582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HLS53096.1"
                     /translation="KVHNIYPNSIYEKDKSILFITKHKGSKFLIILGKSQLLNEFKGR
                     SFPSGQGKICDLTYDNSQILGKYFPFILPKSHKGYDTSIGLGDRLGLASIGHLKLIKD
                     YKAFPVLAQQSIRELNLTDRTYKDVLASASWAVFQEGYTSGFGADGDHLKTAEEVAMA
                     LDLGFTMITLDCSDHIDNSIKDLGKDMVEAEYSKIDKKKRENLEDKYLNKSFSLTDDI
                     EISFDESNFKRIILIYLKTIEFTEKIYRTLIKPLSRKVDFELSIDETSFTTSTEAHFL
                     IGAELRDRKVEIASMAPRFPGEFQKGIDYIGDTEKFSRDFQLHHLIAEHFGYKISVHS
                     GSDKFTVFPIIGEVTKGHYHLKTAGTNWLEALRVIALKDANLFRHIFQFALDNLEKAR
                     AYYHISGDP"
BASE COUNT          420 a          204 c          216 g          363 t
ORIGIN      
        1 aaagtacaca atatttatcc taactctatt tatgaaaagg ataaatctat tttatttatt
       61 acaaaacata aaggttcaaa atttcttatt atcttaggta aaagtcaact cttaaatgag
      121 tttaaaggaa ggtcctttcc atctggccag gggaaaatat gcgatctcac ttatgataat
      181 agtcagatac ttggaaagta tttccccttc atcctaccta aatcccataa aggctatgat
      241 acctccatcg gtctaggtga taggctagga ttagcatcaa taggtcatct taaattaata
      301 aaagattata aggcctttcc cgtattagcc caacaatcta tacgggaatt aaacttaaca
      361 gacaggacct ataaggacgt tttagcatca gccagctggg ctgttttcca agaaggttat
      421 acatctggtt ttggtgctga tggcgatcac ttaaaaacag ctgaagaggt agctatggct
      481 cttgatttag gttttaccat gattacactg gactgctcag accatattga taactccata
      541 aaggatcttg gcaaggatat ggtggaggca gaatattcaa aaatagataa aaagaaacgt
      601 gaaaacttag aagataaata tctaaataaa agtttttctt tgactgatga catagaaata
      661 tcttttgatg agtccaactt caaaagaatt atcctaattt atttgaaaac cattgaattt
      721 acagaaaaaa tctataggac ccttattaaa cctctttcaa gaaaagttga ctttgaactt
      781 tctatagatg aaaccagctt taccacctcc acagaagctc actttttaat aggggcagaa
      841 ttaagagata ggaaagttga aattgccagc atggctccaa gatttcccgg tgaatttcaa
      901 aaaggcatag attatattgg tgatactgaa aaattttcca gggacttcca actacatcat
      961 ttaatagcgg aacattttgg ttataaaata agtgtccatt ctggtagtga taagtttaca
     1021 gtatttccaa taataggtga agttacaaaa gggcactatc acctaaaaac tgcgggcact
     1081 aattggctgg aggccttaag agttatagcc ctaaaggatg caaatttatt taggcatatc
     1141 ttccaatttg ccctagataa tttagagaag gctagggcct attatcatat cagtggagat
     1201 cca
//
LOCUS       DATGSI010000259         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_208777, whole genome shotgun sequence.
ACCESSION   DATGSI010000259 DATGSI010000000
VERSION     DATGSI010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208777"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VK031_03930"
     CDS             <1..1077
                     /locus_tag="VK031_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HLS53097.1"
                     /translation="IIGALIYEGKAKTEDEAVGLVESGEIEFAPCNEHGAVGPMAGIV
                     SPSMPVHIVHNKTYGNYGYCTVNEGLGKVLRYGAFDDEVINRLTWIEEEFAPIMREAI
                     KGCEGGIDIKALISQAVHMGDECHNRNKAATSLFFREIATHVMDVDADEDAKRRVLDF
                     IKANEHYFLNLSMPACKVATDAAHGIENSTIVTTMARNGVDFGIRVSGLGKDQWFTAP
                     ANMIKGLMFPGYTEEDANPDIGDSAITETMGIGGFAMGGSPAIVQFVGGSVEDAINYS
                     ESMYEITTSENSNYSIPTLNFRGSAIGIDILKVVEKGILPIINTGMAHKVAGIGQVGA
                     GLVTPPMECFEKALLAFKENSKRI"
     gene            1087..1998
                     /gene="arcC"
                     /locus_tag="VK031_03935"
     CDS             1087..1998
                     /gene="arcC"
                     /locus_tag="VK031_03935"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HLS53098.1"
                     /translation="MSRILIALGGNALGETPDEQKRLVENTAKNLADLVEKGHEIIIV
                     HGNGPQVGMIEEALNHMPMPFPECTAMSQGYIGYHLQNSIYNELARKNINKNVITTIT
                     QVVVDEKDHAFQNPTKPIGGFYSKEEAKTLEKEKGYTMVEDSGRGYRRVVASPEPIDI
                     VEKESIKHLVDGGNVVIAAGGGGIPVVKKEFGYEGVSAVVDKDLASGKMAELLDADYL
                     FILTAVDKIAINFGKANQENLDKISVSEAYKYMEEGQFGVGSMLPKVMAAVTFVKSKA
                     RRQSIIASLEKAGEALVGESGTRIYNK"
     gene            2201..>2566
                     /locus_tag="VK031_03940"
     CDS             2201..>2566
                     /locus_tag="VK031_03940"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HLS53099.1"
                     /translation="MTTKKYLDFVISKLEEVKKTQITKIEKASDLIVESCIRDGRFYI
                     FGSGHSHMIAEELYIRAGGLAYVKAILPPELMLHEMPNKSTHLERMDGYAKSMLELHK
                     VDSNDTLMVISNSGRNSVPV"
BASE COUNT          893 a          380 c          609 g          684 t
ORIGIN      
        1 ataatagggg ctctaatata tgaaggtaag gcaaaaacag aggatgaggc agtaggcctg
       61 gtggaatctg gtgaaataga gtttgcaccc tgtaatgaac atggggctgt aggacctatg
      121 gctgggatag tatccccctc catgcctgtg catattgttc ataataaaac atatggtaat
      181 tatggatatt gtactgtaaa tgaaggctta ggaaaggtct taagatacgg agcctttgat
      241 gatgaggtta tcaatagact aacttggata gaagaagagt ttgcacctat tatgagagag
      301 gctattaaag gctgtgaagg cggcatagac ataaaggctt taatttccca agctgttcat
      361 atgggagatg aatgccacaa tagaaataag gcggctacca gtctcttctt tagggagatt
      421 gctacccatg taatggatgt ggatgcagac gaagatgcca aaaggagagt tttagatttc
      481 atcaaggcca atgagcacta ctttttaaac ctatctatgc cagcttgtaa ggtggcaaca
      541 gatgcagccc atggtattga aaattcaact attgtaacca ccatggctag gaatggagta
      601 gacttcggta ttagagtaag cggactaggt aaggaccagt ggtttactgc acctgccaat
      661 atgattaagg gcctcatgtt tccaggctat actgaggagg acgccaaccc agatatagga
      721 gacagtgcta ttactgaaac catgggtatt ggaggttttg ctatgggagg ctctcctgca
      781 attgtccaat ttgtcggagg tagtgtagaa gatgctatta attatagtga atctatgtat
      841 gaaataacta ccagcgaaaa ttcaaattat tctataccaa ccttaaattt taggggatct
      901 gctataggaa ttgacatatt aaaggtagtg gagaagggaa tactacctat tataaataca
      961 ggtatggccc ataaggttgc aggtataggc caggtaggtg ctggattagt aactccacca
     1021 atggaatgtt ttgaaaaagc tcttcttgcc tttaaagaaa atagcaaaag gatttaggag
     1081 gttaatatga gtagaatatt aatagcacta gggggcaatg ccctaggtga aacgccagat
     1141 gaacaaaaaa gactggtaga aaacacggct aaaaatttag cagatttagt ggaaaaaggc
     1201 catgaaataa tcatagtaca tggtaatgga ccacaggtgg gaatgataga agaagcctta
     1261 aatcacatgc ccatgccctt cccggagtgt acagccatga gtcaaggata cattggctat
     1321 catttacaaa atagtattta caatgaactg gctaggaaaa atataaataa gaatgtaata
     1381 actaccataa cccaagtggt agtagatgaa aaagaccatg cctttcaaaa cccaaccaaa
     1441 cctattggtg gattttactc taaagaggaa gctaaaactt tagaaaaaga aaagggctat
     1501 acaatggtgg aagactcagg aagaggatat agaagggtag tagcttcccc tgaacctata
     1561 gatatagttg aaaaggagag tataaagcac ttggtagatg gtggcaatgt ggttatagct
     1621 gccggaggtg gaggaatccc tgtagttaag aaagaatttg gctatgaagg agtaagcgca
     1681 gtggtagaca aagatttggc cagtggcaag atggctgaac ttctagatgc agattatcta
     1741 tttatcttaa ctgcagtaga taaaattgcc attaattttg gcaaggcaaa ccaggagaat
     1801 ttagataaaa tatctgttag tgaggcctat aagtatatgg aagagggcca atttggtgta
     1861 ggcagtatgc tacctaaggt tatggctgct gtgacttttg taaaatctaa agcacgaaga
     1921 caatcgatta tagcctcctt agaaaaggca ggagaggcct tggtaggaga aagtggtacc
     1981 agaatatata ataagtaata aaaggaagct ctagctaggg cttcttttta tgatatttgt
     2041 ggaaaactgc aaaaaaattt gatatattga ttataaactt aagatttaag atagggtaag
     2101 ttcttgtccc atttattgta gagttttata aacagttaca cttagtcaaa ttgggtatag
     2161 actagattga aaggatcatc tatataaggg ggctgtagat ttgacaacta aaaaatattt
     2221 agactttgtc atatcgaaat tagaggaagt gaaaaagact caaataacaa agatagaaaa
     2281 agcttcagat ctgattgtag aatcttgcat aagggatgga agattctata tatttgggtc
     2341 aggtcactca catatgatcg ctgaagagct gtatataaga gctggagggc tagcttatgt
     2401 aaaggcaata ctacctccag aattaatgct acatgaaatg ccaaataaaa gtacacatct
     2461 tgaaagaatg gatggctacg caaagtctat gctagaattg cataaggtag attctaatga
     2521 taccttgatg gtaatatcaa actcaggaag aaatagcgta ccagtt
//
LOCUS       DATGSI010000260         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_64611, whole genome shotgun sequence.
ACCESSION   DATGSI010000260 DATGSI010000000
VERSION     DATGSI010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64611"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VK031_03945"
     CDS             <1..510
                     /locus_tag="VK031_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53100.1"
                     /translation="WRHQYLEYLQNKGIDGPIPNYLSLAPMSNGEIFNRPIQIRKTKD
                     DIPITKIRNSPLRVITVGKTFVDMLRDPNMCGGEDHVLDVFINEAPRFQNEIIRYVDR
                     NGRAIDKSRIGFIFDKILGIKGPKIEQWKQEQVRGGSKKLSPNFPFSSIFDEGWCLSI
                     NIESAQQYI"
     gene            514..>1015
                     /locus_tag="VK031_03950"
     CDS             514..>1015
                     /locus_tag="VK031_03950"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLS53101.1"
                     /translation="MFTENKLINIEDWVDSGGPIRDVDYRACVHIILTGISINHRLAD
                     SMIMKGGSLLALRYKSKRHTTDVDFSTRLKLEEQEITSLLSELSNSLERARVALGYNQ
                     IRCAVQSHQIKPNKDASFPTLQIRVGYARADTRNEVKRLEAGESRRVVRIDYSFNEKT
                     YNTEKIL"
BASE COUNT          339 a          173 c          232 g          271 t
ORIGIN      
        1 tggcggcacc agtatttaga gtatttgcaa aataaaggaa tcgatggccc catccctaat
       61 tacctctcct tagcccctat gagtaatggc gaaatattta ataggccaat acagatacga
      121 aaaacaaaag atgacatacc catcactaaa ataaggaata gtcctcttag agtaatcact
      181 gttggcaaga cctttgtgga tatgcttaga gatcccaaca tgtgtggtgg agaggatcat
      241 gttttagatg tatttattaa tgaagcgccg cgattccaaa atgaaataat taggtatgta
      301 gatcgaaatg gtcgagcaat agataagtcc cgtattggat tcatattcga caaaatcctt
      361 ggtataaaag ggcccaaaat agaacagtgg aaacaagagc aagtaagagg aggatcaaaa
      421 aagcttagcc ctaactttcc attttcatct atttttgatg aggggtggtg cctatccata
      481 aacatagagt cagcacaaca atatatttaa attatgttta ctgagaacaa actaataaat
      541 atagaagact gggtagatag tggtggccct attagagatg ttgattatag agcgtgtgta
      601 catattattt tgacggggat atctataaac catcgactgg cagatagtat gattatgaaa
      661 ggaggatctc tgctggcgct tagatacaag agcaagcggc acacaacgga cgtggatttt
      721 tctaccagac tcaagttaga agagcaggag attacttctc ttttatctga attatccaat
      781 agtttggaaa gggctagagt ggcgcttggg tataaccaga ttcgatgtgc ggtacagtcc
      841 catcagatta aacctaataa ggacgctagt ttcccgacgc tgcaaattcg agtgggctac
      901 gctagagctg atacacggaa tgaggtaaaa cgtcttgagg caggggagtc taggcgggtt
      961 gttcgcatag actatagctt taacgaaaaa acgtataata ctgagaaaat acttt
//
LOCUS       DATGSI010000261         5474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_586764, whole genome shotgun sequence.
ACCESSION   DATGSI010000261 DATGSI010000000
VERSION     DATGSI010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586764"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VK031_03955"
     CDS             complement(<1..184)
                     /locus_tag="VK031_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HLS53102.1"
                     /translation="MEFKDYYKILGIDKNASDAEIKRAYRDLAKKYHPDLHPNDKNAQ
                     AKFQEINEAYQVLGDKE"
     gene            complement(327..488)
                     /locus_tag="VK031_03960"
     CDS             complement(327..488)
                     /locus_tag="VK031_03960"
                     /inference="COORDINATES: protein motif:HMM:NF020945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HLS53103.1"
                     /translation="MMEKINTLKRDLNNALEAKEPTSQEVLRISEELDLLILQYYQEH
                     GKKNGSKNI"
     gene            763..1176
                     /locus_tag="VK031_03965"
     CDS             763..1176
                     /locus_tag="VK031_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLS53104.1"
                     /translation="MNNNRIDPIESSLDLINIGQRIRIEREKLGITRERFAEIVNLSP
                     FYIGQIERGERSMSLDTLDKISKALKISLDYIITGNVPYHNHGLVLESIEDYYGRENS
                     EELEELLDLLFKLPQDKIALINDIIKILLPHLNMQ"
     gene            complement(1235..1504)
                     /locus_tag="VK031_03970"
     CDS             complement(1235..1504)
                     /locus_tag="VK031_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HLS53105.1"
                     /translation="MMEAKKRAANKLKTARGQIDGIIRMVEEDRYCVDISTQILSVIG
                     LLKKANIDVLNSHIRSCVANAILEGESQGEEKIEEIIHVIDRYIK"
     gene            complement(1519..1725)
                     /locus_tag="VK031_03975"
     CDS             complement(1519..1725)
                     /locus_tag="VK031_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HLS53106.1"
                     /translation="MKKILTVEGMSCVNCENAVKKVLNELEEVTGVEVDLGKKLVEVE
                     GNNLDDDKLKAVIDEAGYDLIKID"
     gene            complement(1747..4119)
                     /locus_tag="VK031_03980"
                     /pseudo
     CDS             complement(1747..4119)
                     /locus_tag="VK031_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485948.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            4319..4543
                     /locus_tag="VK031_03985"
     CDS             4319..4543
                     /locus_tag="VK031_03985"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HLS53107.1"
                     /translation="MDRSPLTSLEPNEVGIIMEICGGCRARRRLYELGLNKGTKVRMV
                     KNDIGPVILNLYGHKLALGKGLANKILVEK"
     gene            4585..>5474
                     /locus_tag="VK031_03990"
     CDS             4585..>5474
                     /locus_tag="VK031_03990"
                     /inference="COORDINATES: protein motif:HMM:NF020990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HLS53108.1"
                     /translation="MNTIALVGNPNSGKTTLFNALTGSNQHVGNWPGVTVEKKEGKIE
                     YKGKEYKIVDLPGTYSLGAHSEDEVIARDFILKGNPDIVINVVDATNIERNLYLTTQL
                     MEMDVKVVLALNMMDEAKSHNIQIDINKLSHALGIPIVATVASKKIGIEELIENSIRA
                     IEDSKPREQISYYDGDIAEKINNVKSYLKGAVLDYPTDWVAIKLLEGDNEIINHVREH
                     SPDKLENKVLEISGDLEECELQIISNRYDFISNIVKESIVKPSEYKETLTDKIDKIVT
                     HKIWGLPIFAVIMFVIFQLT"
BASE COUNT         1611 a         1100 c          858 g         1905 t
ORIGIN      
        1 tttccttatc tcctaatacc tgataggctt cattaatctc ctgaaatttg gcctgggcgt
       61 tcttatcatt tggatgtagg tctggatgat atttttttgc caagtcccta taggctcttt
      121 ttatttcagc atcactggca tttttatcaa tacctaggat tttataatag tccttaaatt
      181 ccattaaatc atctccccaa ttagagataa gtatttgacc ttatttgacc ttatctgacc
      241 ttgcctttat aattatactt gttttatcta taaatttcaa tgaaaaaagc aacctaactt
      301 aggttgccat ctgtaccttc aaggacttaa atatttttag aaccattttt ttttccatgc
      361 tcttgataat attgaagtat cagtaaatcc aattcttcgc taattctcaa tacctcctgg
      421 ctcgttggct ccttagcctc caaggcatta tttaaatccc ttttaagtgt gttaatcttt
      481 tccatcatac taagccacct cagggaattg actaatattt gttttctttt attaacatct
      541 tcatctaatc ataaaaaata gtgtatcact ttttcttaaa attagctagg tataaattta
      601 actgtaacct aagcctatta ttttacccta taatataata tactaatctt ctatagattt
      661 caacactttt atatgcttat tgccaatctt ttttgtatgc aactagcccc tttcaaatga
      721 cacaattagc ctttacagtt aaaataaggg ggaattatac cagtgaataa taatagaata
      781 gatccaatag aaagttcact agacttgatc aatattggcc aaagaataag gattgaaaga
      841 gaaaaattag gaataactag agaaaggttt gcggaaatag ttaatttgtc tcccttttac
      901 ataggacaaa tagagcgcgg ggaaaggagt atgagtttag atactttaga caaaatttcc
      961 aaggccctaa aaatatcact agattacatt ataacgggaa atgttccgta tcataatcat
     1021 ggattagttc ttgagtctat tgaagattat tatggtaggg aaaattcaga agaacttgaa
     1081 gaactactgg acctgctctt taaattgcct caggataaaa tagctttaat aaatgatatt
     1141 ataaaaatat tacttcccca tttaaacatg caatagttct tttacctaaa taacccagcc
     1201 ctggtcctag gataagggct gggttatttc tcttttattt aatatatcta tcgattacat
     1261 ggataatctc ttcgatcttt tcttctcctt ggctttctcc ttctaaaatt gcattggcta
     1321 cacaggatcg aatgtgacta tttaacacat ctatatttgc cttttttaat agacctatga
     1381 ctgataaaat ttgggtagaa atatctacac agtatctatc ttcttccacc attctaatga
     1441 tgccgtctat ttgtcctctt gcagtcttta gtttgtttgc agctcttttt tttgcttcca
     1501 tcataaaatc accaatttct aatcaatttt tattagatca tatcctgctt catctattac
     1561 cgcttttagt ttatcatcat ctaaattatt tccttcaact tctaccaatt ttttacccaa
     1621 atccacttct actccagtaa cttcttccaa ttcatttaag actttcttaa ctgcattttc
     1681 acagtttaca catgacatgc cctcaactgt taaaatcttt ttcatttaaa tctacctcct
     1741 atatttttat ccttcaaacc tttttaatct cagtgagttg gttactacag atactgaact
     1801 gaaagccata gctgctcctg ctaccattgg gtttaaaaat cctaaggctg agaaggggat
     1861 tcctactgta ttgtagaaaa aggcccagaa taggttttgt tttatggttc tcatggtcct
     1921 atggcttagt ctaatcgctg ttacaactcc tgttaaatct cctctcatta gggtaatatc
     1981 agctgcttcc atagccacat ctgtacctgt acctatggca aagccaacat ctgacgcggc
     2041 caaggctggg gcatcattta taccatctcc caccatacca acatgcttgc cttcacttct
     2101 caatttctcc actgcttctg ccttattttc cggtagtaca tcagccaata cattggtaat
     2161 tccaatctgc cttgcaatag cctgggctgt cctttcattg tctccagtta tcatatatac
     2221 ctccaagccc atatccttta aatcccttat ggcctgtagg gaggtatctt tcatttggtc
     2281 tgccacagct attataccag ttattttgcc ttctatggca actaacatgg cagttttacc
     2341 ttcctcctcc agcttcaata gatcattttc cttatcctct atctgaacct ggttttctct
     2401 cattagcctt ctgttaccta ttaaaatttt ctgtccttca aatactgctc ttaaaccttt
     2461 acccggtatt gcttcaaagt cctttggtgc cactatatct aggccctctt cttcagcctt
     2521 tcttactatg gcctggccta aggggtgttc tgaggccttc tccacagatg ccgcaattct
     2581 taatatttct tcattaccat aatctttaaa agccattata tctgtaactt caggctctcc
     2641 tcttgttatg gttcctgttt tatcgaaaat tattgtatcc atcttgtgag ctctttctag
     2701 gtgttcccca gatttaatta aaataccatt ttctgcaccc ttgcctgttc ctaccattat
     2761 ggctgtaggt gttgctaagc ctagggcaca ggggcatgca attactaata ctgccactgc
     2821 attaattatt cctgtattaa aatcgccgta aaggtaaaac cctatcactg ttattagggc
     2881 tataagcaca actactggca caaatatacc ggaaatttta tctgccagcc tttgtactgg
     2941 tgccttagat ccttgagcat cttcaactaa ctttataatc tgagaaagta ctgtgtcctt
     3001 acctattttc gttgccctga atttaaagga tccaaaccta tttatggttg cacctataac
     3061 ttcatcatct actgtcttgt ctataggtat actttctcct gtaatcatag actcatctag
     3121 ggctgaattt ccttccacaa taataccatc cactggaacc ctttcaccag gtcttacaac
     3181 tactatatca cctatttgta actcttctat agggatttct acctccctgc catccttaat
     3241 tattctagca gtttttggtt gcagtcccat caacttcttt atagcttcag aggttctgcc
     3301 ttttgccaca gcttcaaagg tctttcctag aagtattaaa gtaataatta ctgcagaagc
     3361 ctcaaaatag aattcaggta tatccattaa taggttatag atactgtaaa agtaggctgc
     3421 tgaagttccc atagctacca gcacatccat atttgctcct ccacctctta gggcattaaa
     3481 ggccccttta taaaacctat aacctataat aaattgtact ggagtggcca aggccaactg
     3541 gaaccagcca ttgcctaaaa tattgtctat tccagccatg tggaagaaca tggcagaaaa
     3601 taaaggaaag cttaagatgg cagatataat aaagtctctt ttcagagttt ttatctcctg
     3661 ctctcttagt ttttgttccc tgtctaagtc ctgctctatt tctaattctg cttcatatcc
     3721 tgctttcttt acagtttcaa ttaaggtttc ttcatctact gttcctgaag cgtactccac
     3781 caatgcctta ttggttgata aattcacatt tgcccttact actccttcta acttatttaa
     3841 agccttctcc actctggtgg aacaggctgc acaggtcatt ccctgaatta agagcgtatt
     3901 ttggaccaga ggtaccttaa aacctgtctt ttctattact tccactaagt cctttggttt
     3961 aataacttct tcatcatatt ctattgttgc tttttcacct aaaaggttaa ctacagagtc
     4021 taaaactcct tctgtcttgt tgagtgcccg ctcaactcta ctggaacatg aggctcatgt
     4081 catgccagaa actattatat caattttttt acttgccatg atatcatcat cctttcttac
     4141 ccccctccct gggggtgtgt gtattatatt agcacctttc ttttattttg tcaaatattg
     4201 tctaacttaa ttctaaacga ttgtcccttg attttccttg gaaaatagta tattatacta
     4261 ttgataatga ttatcattca caagtagtta gtttatacat attgaaagga agaatgctat
     4321 ggatagatcc cctttaactt cattggaacc taatgaagtg ggaattatta tggaaatatg
     4381 tggaggttgt agggccagaa gaagattata tgagttaggc ctaaataaag ggactaaagt
     4441 aaggatggtt aaaaacgata ttgggcctgt aatccttaac ctttatggtc acaagcttgc
     4501 cttaggtaaa ggcttggcca ataagatact ggtggaaaaa taaatacatt taaaagactg
     4561 caatcaattg agaggaatga tttaatgaac acaattgcat tggtaggcaa ccctaatagt
     4621 gggaagacta ctttatttaa tgccttaacc gggtctaacc aacatgttgg aaattggccc
     4681 ggtgttactg ttgaaaaaaa ggaaggaaaa attgaataca agggtaagga atacaaaatt
     4741 gtggacctac ctggtacata tagtttaggg gcccattcag aggatgaagt aatagctaga
     4801 gattttattt taaaaggtaa ccctgatatt gtaattaatg tagtagatgc aactaatatt
     4861 gaaagaaatc tatatcttac aactcaactg atggagatgg atgtaaaggt tgttttagct
     4921 ttaaatatga tggacgaggc caaatctcat aatatccaga ttgatattaa caagctatct
     4981 catgccttag gaattccaat tgtagccact gttgcatcaa aaaagatagg tatcgaagag
     5041 ctaattgaaa actccattag ggccattgaa gattctaaac caagagaaca gatctcctat
     5101 tatgatggag acatagcaga aaaaatcaat aatgttaagt cttatttaaa gggagcagtt
     5161 cttgattatc ctacagattg ggtggcaatt aagcttttag aaggtgacaa tgaaataatc
     5221 aaccatgtga gggaacatag tcctgataag ttggaaaata aggttcttga aattagtggg
     5281 gatctagaag aatgtgaact tcaaataatt agtaaccggt atgattttat atcaaatatt
     5341 gttaaggaaa gtatagttaa gccatccgaa tataaggaaa ctttaactga taagatagat
     5401 aagatagtaa cccataaaat ttggggacta cctatatttg cagttattat gtttgtaatt
     5461 ttccaattaa catt
//
LOCUS       DATGSI010000262         5153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_347473, whole genome shotgun sequence.
ACCESSION   DATGSI010000262 DATGSI010000000
VERSION     DATGSI010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347473"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="VK031_03995"
     CDS             complement(<1..1345)
                     /locus_tag="VK031_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53109.1"
                     /translation="MGGLHEQSESTGRGNDTGGAYIQLTLFPTEQEQIQRIAGKERIN
                     KPFAYSAFSMPQNQMDSILRTGNNKDDGLLRIISFYQKNKSLEEKADFLQKEYQGGKG
                     LYIENQKVSLWFYDDGIHIAKGDTALYSNSKQVISWIEVAERIEELLRDGQYVPQDIL
                     DKVGAYERQELAQTLWYLHQDFSDKARKQFFNEEIFKGGFPDSTKHIAQLLDSPKERE
                     IIIESLQQFNFAYEEDPKLLRFHFHKPQKLLSDIKDLQLPRREFNANITEISDYKMFI
                     TQDEVDKELTSGSGIQGGKFRIYEFFTEPHTPKEKEDFLKQEYGTGGRSHALSGADNS
                     TQMHDAKGIVYSRRDGDDKLHLNWSKVTKRVDYLISTDRYITAEEKVRLEGIQRDKAG
                     LTESPPIPEITEPMEETSQEMTQDTTEEIIPIPYSKGDTIYLENGTPFLIEEITYY"
     gene            complement(1273..1632)
                     /locus_tag="VK031_04000"
     CDS             complement(1273..1632)
                     /locus_tag="VK031_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HLS53110.1"
                     /translation="MKEITYRRNGDYLIPNIELTGQKESKDQSPLGKYGRMRKQYLQE
                     HRKPLFHSLLLSEKLLAHLLEIDKTANRRMGQIMIDLQKVQTPPDKMTDQMEWVGYMN
                     NLKAQAEEMILAELIYN"
     gene            complement(2530..2991)
                     /locus_tag="VK031_04005"
     CDS             complement(2530..2991)
                     /locus_tag="VK031_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53111.1"
                     /translation="MRKTLKQKIDEADKRDINIPDDLLADENIVGVYGFFAIKRDEEY
                     CFYIGKATNVAYRLLGSSNGHVYLYLKNDYSKLVPQKINEYLNDKYKIEVRILDKIDY
                     HDTSFSKAAHRLALAELQRIVQYQEQGQCEFQVPEGSGTYERKYWEKHYKK"
     gene            complement(3058..3276)
                     /locus_tag="VK031_04010"
     CDS             complement(3058..3276)
                     /locus_tag="VK031_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HLS53112.1"
                     /translation="MDKFTVEETNLICIYNTGTRESLLEELSEMQTHLEQDETELIEL
                     TKSVINKISAMSDGDFEDVIVELVADFN"
     gene            complement(3279..4454)
                     /gene="radC"
                     /locus_tag="VK031_04015"
     CDS             complement(3279..4454)
                     /gene="radC"
                     /locus_tag="VK031_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HLS53113.1"
                     /translation="MRDTDELRYIQAFARIIGVKEDAAIEYAERKGLNALVDNATQLL
                     ATPVQQEKHQAFLDLYRMSSTINTRNPIINSPEKASSYFHSVMDEIYDKEAFVVAFLN
                     TKNRVMDHEVVSVGTINSSLVHPREVFRNAIINKANSVILCHNHPSGDLTPSTEDINI
                     TRRLKETGNLLGIKVLDHLIISGINQNEVYSFQAKGVLEVVAAYGINSSQSVGEIKSD
                     YMMPMQEHSYPMPDPNMSISDRNSHGYLDDEMLPLSKGRATELFKENLPVFLLYEDNS
                     EAIAFDLKDIDNHNGIFGIERISWIDQKNYLETAEKSVEQNYNQIDGIINNKPTVEEL
                     EQTVNAGGNISLLDLANSVKAEREEKKTSIVKQLKAKPAIKKEKQKRLPQIGTEMER"
     gene            complement(4455..4625)
                     /locus_tag="VK031_04020"
     CDS             complement(4455..4625)
                     /locus_tag="VK031_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53114.1"
                     /translation="MSIKNLIKTLLDIEVEADNLLELRDNPSKYVTKKEDIEKLNDLF
                     LLIDLLDKQEVE"
     gene            complement(4630..>5153)
                     /locus_tag="VK031_04025"
     CDS             complement(4630..>5153)
                     /locus_tag="VK031_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HLS53115.1"
                     /translation="HFIPTHKGISLITILPEIIQSPILTAEWEEKLKQIEQGSLSPDE
                     FLQEINEMIESLVRTYEVIKGTDNLFPSNKEGIGKCPRCGGNVIENRKVFCCRNKDCA
                     FALWKENKFFTMKKKTLTKEIAIKLLKEGRVKLTGCYSEKTGKTYDAIVILDDNGGKY
                     INFKIEFPAKKGR"
BASE COUNT         1420 a         1037 c          751 g         1945 t
ORIGIN      
        1 tataatacgt aatttcttca attaaaaaag gtgtaccatt ttctaaatat atggtatcac
       61 ctttactata gggaatagga ataatttctt ctgtagtgtc ctgtgtcatt tcttggcttg
      121 tttcttccat aggctctgtt atctcaggta tgggtggtga ctctgttaac cctgccttat
      181 ctctttgtat tccttcaagc cttacctttt cttcggcagt tatataacgg tcagtggata
      241 taaggtaatc aactcgcttg gtaactttac tccagtttaa atgtaatttg tcatccccat
      301 ccctacggct atatacaatg ccttttgcat cgtgcatttg agtgctatta tcagctccag
      361 aaagggcatg acttctgccc cctgtaccat attcttgctt taaaaaatct tctttttctt
      421 taggagtgtg tggctctgtg aaaaattcat atatacgaaa tttaccgcct tggataccac
      481 ttccgctagt aagttcctta tctacttcat cctgtgtgat aaacatctta taatcagaaa
      541 tctcagtaat atttgcatta aattcccgtc taggtagttg taagtcttta atatcagaaa
      601 gaagcttttg aggtttatgg aaatggaaac gcaaaagttt agggtcttct tcataggcaa
      661 aattaaactg ttgtaatgat tcaataatta tttccctttc ctttgggcta tctagtaact
      721 gtgctatatg tttcgttgaa tcaggaaatc cccctttaaa tatttcttca ttaaaaaatt
      781 gctttcttgc cttatcagaa aaatcctgat gcagatacca tagtgtttgt gcaagttctt
      841 gtctttcata ggctcctacc ttatctaata tatcctgcgg tacatattgt ccatctctta
      901 aaagttcttc aatcctttca gcaacttcta tccagctaat tacttgcttt gaattagaat
      961 agagggcagt atctccctta gctatatgaa taccatcatc ataaaaccat aaagatactt
     1021 tctgattttc aatataaagt ccttttccac cctgatattc cttttgtaaa aaatctgcct
     1081 tttcttctaa tgatttattc ttttgataaa atgaaataat tcttagtaag ccatcatcct
     1141 tgttattccc tgtccttaat atggagtcca tctgattttg tggcatagaa aaagcggagt
     1201 aggcaaaagg tttattaatc ctttccttcc ccgctatcct ttgtatctgt tcttgttccg
     1261 taggaaacag agttaattgt atataagctc cgccagtatc atttcctctg cctgtgcttt
     1321 cagattgttc atgtaaccca cccattccat ctggtctgtc atcttgtccg gtggggtctg
     1381 tactttctgc aaatctatca ttatttgccc catccgcctg ttcgctgtct tgtctatctc
     1441 caacaggtgg gctaacagtt tctccgatag gagtagggag tggaataatg gtttcctgtg
     1501 ttcctgaaga tattgttttc tcatcctgcc gtatttgccc agtggactct ggtctttgct
     1561 ctccttctga cctgtcaatt ctatgttggg tatcagataa tctccgttcc ttctgtaagt
     1621 tatttctttc attttcctcg ctcctctcac gctctgtttt aataatagta atagctattt
     1681 ctctaaatat ttcctctgat tgctcactaa ttgctgtacc taataaggcg atggtatcag
     1741 cagtgttaaa attaaatacg ggtagaaaat cctcatgtcc aaaatatcca tctgtatcta
     1801 agccacatct tttcattagg gtgtatgctg tactgacagt tgctgcatct tcaaaagcct
     1861 tttgtaggtt atcctcatca tattcctcta aagagctatt ttctataaga taacccatat
     1921 cccttttatg attattataa tactccctgg acatatttct tgcaatctta taaaaggcat
     1981 caaaaagatt attttcctct acattatact tatcctttaa catatctaaa atcggctctt
     2041 tgtgatcttc tttcatatcc caaagataag gataacggga attttccctg ccccctgtat
     2101 cagaaacatc aaaaacatat cttagccttg gaatatcagc tgtcgtatca agcaatgcaa
     2161 ttccctttga gccacggcga acatacctat tcatagtgtt attccaaagg tcatattctg
     2221 cacaagccct agcatcaggt ctttgtgcat aaatcattag ctgttcatga taagggtatt
     2281 tatatagcct tccaacagta ttaagaaaac ctgtccaatc ttcaaggctc ctagttaagc
     2341 ctttggctgt atcttctgcc aattgtgcat aggtttgtat tttagttgcc atatattaat
     2401 cctcctttat ttttgcacag aaaaaagcaa ctcacctgct aaaaagggag ttgcttttat
     2461 gtctatgtta atagtgaatt agctaacaaa ttggatttca tttaaaacaa tcaatcttta
     2521 ttcatttcac tattttttat aatgtttttc ccaatatttt ctttcatacg tccctgaacc
     2581 ttctggcact tgaaattcgc actgtccttg ttcttgatat tgaactattc tttgaagttc
     2641 cgctagagca agacgatgag cagcttttga aaaagaagta tcatggtaat caatcttatc
     2701 aagtattctt acttcaattt tgtatttatc attaagatat tcattaattt tttgtggaac
     2761 taattttgaa taatcatttt ttaaatataa ataaacatgc ccattactag agcctaacag
     2821 tctatatgct acgttagtcg cttttcctat atagaaacaa tattcttcat ctcttttaat
     2881 tgcaaaaaat ccatatacac cgacaatatt ttcatcagcc aataaatcat caggaatatt
     2941 gatatctcgt ttgtctgctt catctatctt ttgttttaat gtctttctca taaactactc
     3001 cctaaattcc tatttgtttg tatcttacta cgaaatctaa cttatcacaa caaatagcta
     3061 attaaaatca gccactaatt ctactataac atcctcaaaa tcaccatcac tcatagcact
     3121 tatcttattt ataactgatt ttgtaagttc tataagttcc gtttcatcct gttctaagtg
     3181 ggtctgcatt tcagatagtt cttcaagcaa gctttccctt gtgcctgtat tgtagataca
     3241 tattaaattt gtttcctcta ctgtaaattt atccataatc atcgctccat ttctgtacct
     3301 atttgaggta atcttttctg cttttccttt tttatagctg gttttgcttt aagttgcttt
     3361 actatagagg ttttcttttc ttctctttct gcctttactg aattggcaag atctaataat
     3421 gaaatattac cacctgcatt tacagtctgc tccaattcct ccactgtagg cttgttatta
     3481 attattccat ctatttgatt atagttttgc tccactgatt tctcagcagt ttctaaatag
     3541 tttttttggt ctatccagct aatgcgctca ataccgaaga taccattatg gttatctata
     3601 tcctttaggt caaaagcaat tgcctcagaa ttatcttcat agagtaagaa tacaggtaaa
     3661 ttctctttaa ataattctgt agctcgtcct tttgatagtg gtagcatttc atcatctaag
     3721 tatccgtggg agtttctatc tgaaatactc atattaggat caggcatggg atagctatgt
     3781 tcttgcatag gcatcatata atcagatttt atttccccta ctgattgtga tgaattaata
     3841 ccataggctg ctactacttc tagtaccccc ttagcttgga aggaatatac ttcattttga
     3901 ttaatgccac taataatcaa atgatctaaa accttaatac ccaacagatt acctgtttct
     3961 tttagccttc ttgtaatatt aatatcttct gtacttggtg ttaaatcccc tgatggatgg
     4021 ttatgacaaa gaatcacaga gttagccttg ttgattatag catttctaaa gacttccctt
     4081 gggtgtacca gtgagctatt aatagttcca acggatacaa cctcatggtc cataacacga
     4141 tttttagtgt ttaaaaaggc aactacaaag gcttccttat catatatttc atccattact
     4201 gaatgaaaat aagatgaagc cttttccggg gaattgatga taggatttct agtattaatg
     4261 gtactactca ttctatataa atcaagaaaa gcctgatgtt tctcctgctg aactggggta
     4321 gcaaggagtt gggtagcatt gtctaccaat gcatttaatc cttttctctc agcatattct
     4381 atagcagcat cttcttttac accgataatt cttgcaaagg cctgaatata acgaagttca
     4441 tcagtgtccc tcatttattc cacctcctgc ttatctagca aatctatgag taaaaataag
     4501 tcatttagct tttctatatc ctctttttta gtaacatatt tacttggatt atcacgtagt
     4561 tccagtaagt tatcagcttc tacttctata tctaaaagag tcttaattaa atttttaata
     4621 ctcatgcttt taccttcctt tctttgcagg aaactctatt ttaaagttta tatacttacc
     4681 accattatca tcaaggataa caatagcatc ataggtttta cctgtctttt ctgaataaca
     4741 gcctgtaagt tttaccctgc cttcttttaa aagtttgatt gctatttctt ttgtcaaagt
     4801 ctttttcttc attgtaaaaa atttattttc tttccaaagt gcaaaggcac aatccttgtt
     4861 tctacagcaa aatactttcc tgttttcaat tacatttccg ccacatctgg ggcatttacc
     4921 gataccttct ttattagatg gaaatagatt atctgtcccc tttattactt cataggttct
     4981 tacaaggctt tctatcattt cattgatttc ctgtaaaaat tcatctggag aaagactacc
     5041 ctgttcaatc tgttttagct tttcttccca ctcggcagta agtataggtg attggatgat
     5101 ttctggcaaa atggtaataa gggaaatacc tttatgtgta ggaataaaat gtt
//
LOCUS       DATGSI010000263         1114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_586779, whole genome shotgun sequence.
ACCESSION   DATGSI010000263 DATGSI010000000
VERSION     DATGSI010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586779"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..495
                     /locus_tag="VK031_04030"
     CDS             49..495
                     /locus_tag="VK031_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLS53116.1"
                     /translation="MSKKVVKDIKEIEKYLRKIDYIIRVEGRAIINQLNITIPQFTAL
                     QILINHGNMTIGQLSQRMALACSTVTDLVDRMEKNQLVVRKKDKNDGRLVIVEPLPKG
                     HEILEQVLIKRREFLGSMLVKLDKEEVDQLRHGLESLYKIMDISEG"
     gene            601..>1114
                     /locus_tag="VK031_04035"
     CDS             601..>1114
                     /locus_tag="VK031_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLS53117.1"
                     /translation="MAVTIKDVAKMAGVSISTVSRVINDSKPVSPESRRKVLHAIETL
                     GYEPNEIARSLVTKRSNLIGVIVDDIGNYYISQIVRGIEEIGRMYNYDIVLCSSYGDA
                     ETELKFLQLLRSKQVEGVIIASEIINEELIDLVDKLRIEIMYLNRYYSIPELPTITID
                     TEKAINLMMDH"
BASE COUNT          430 a          120 c          243 g          321 t
ORIGIN      
        1 acctagggga atgaaaatat ttatctaata tttaagggga gtatttaaat gtctaagaag
       61 gtagtgaagg atattaagga aattgaaaaa tatttaagaa agattgatta tattatccga
      121 gtagaaggac gggcaataat aaaccaacta aatataacta ttccccaatt cacagccctg
      181 caaatcctaa taaatcatgg aaatatgaca ataggccaat taagtcagag aatggctcta
      241 gcatgtagta cagtaacaga tctggtagat aggatggaaa aaaaccaatt ggttgttaga
      301 aaaaaggata aaaatgatgg ccggttggtt attgtggagc ctttgcctaa gggacatgaa
      361 atattggagc aggtgttaat aaagcgaagg gaatttcttg gaagtatgtt ggtaaaacta
      421 gataaagaag aagtggatca gttaagacat ggcttagagt ccttgtataa aataatggat
      481 atatctgaag gttagttttt ataataaaat gcgaggaata gctttactaa ttaatatgtg
      541 ataatatatt taaagataac agtggtaatg atatggattt aactggaggg gagtgataaa
      601 atggctgtaa ctattaaaga tgtggcaaaa atggctggtg tttccatatc cacagtatct
      661 agagtgataa atgattcgaa accagtaagc cctgaatcta ggagaaaagt acttcatgcc
      721 atagagactc ttggttatga acctaatgaa attgccagaa gtctggtgac aaaaaggtct
      781 aatttgatag gagtaattgt tgatgatata ggcaattact atatatcgca aatagtgaga
      841 ggtatagaag aaataggacg aatgtacaat tatgatatag tcttatgtag tagttatggt
      901 gatgcagaaa ctgaattgaa atttttacaa cttctaagga gcaagcaggt tgagggtgtt
      961 ataatagcat cagaaataat taatgaggaa ttaatagatt tggttgacaa actaaggatt
     1021 gagataatgt atttaaaccg atattattct atacctgaat tgccaacaat aacaatagac
     1081 actgaaaaag caattaattt aatgatggat catt
//
LOCUS       DATGSI010000264         1322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_539091, whole genome shotgun sequence.
ACCESSION   DATGSI010000264 DATGSI010000000
VERSION     DATGSI010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539091"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VK031_04040"
     CDS             <1..560
                     /locus_tag="VK031_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLS53118.1"
                     /translation="FLYNTLNTIIGLSYKDGEKTREALGNLAIYLRGKLNVHSDQYLV
                     PLKEELELVKAYLEIEKLRYGSKLQVEYDMDQNIFVLIPSLTIQPLVENSIRHGLRMK
                     KEDWQIRISTRRVGEKIYITVEDNGIGMSKEKKDQLLAGNSEGVGFKNVLKKIQRIKN
                     ASIELESRESEGTRVIIIMPGGEIL"
     gene            680..>1322
                     /locus_tag="VK031_04045"
     CDS             680..>1322
                     /locus_tag="VK031_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLS53119.1"
                     /translation="MKIKDPDVLFLDIEMGPINGLEISEYFLQEKESLEIIFVTAYPQ
                     YAVGAFEIDAIDYLLKPVQKSRLDMAIKRLKERLSTSAYSSSKKINRDLRINSFGSFQ
                     VLDRENKPLVWRTKKGKELFAYLWAQEGQPATRDQVMEHVFSHIDNNRGSALLHTTIY
                     QIRKGLSNLGYLDGIIYTNDGYKLNLPITSDLDDLTTILKKEKYNRDDIIEILE"
BASE COUNT          506 a          175 c          287 g          354 t
ORIGIN      
        1 actttttata taacacctta aatactatca taggccttag ttacaaagat ggtgaaaaaa
       61 ctagggaggc cttgggcaac ttggccatat atttaagagg aaaattaaat gtacacagtg
      121 atcaatatct agttccttta aaggaggaac tggagttggt aaaggcctac ttggaaattg
      181 aaaaattaag gtatgggtct aaactacagg ttgaatatga tatggaccaa aacatatttg
      241 tcctaatacc atcgttaact attcagcctt tggtggaaaa ttcaattaga catggcttaa
      301 ggatgaaaaa agaagattgg caaataagaa taagtactcg aagggtaggg gaaaaaattt
      361 atattaccgt tgaagacaat ggaatcggaa tgtccaaaga gaaaaaagat caactgctag
      421 cagggaatag tgaaggtgta ggatttaaaa atgttttaaa gaagatccaa agaataaaaa
      481 atgcctccat agagttagaa agtagggagt ctgaaggaac aagagtgata atcataatgc
      541 cggggggaga gatattatga aggtaatttt aatagacgat gaggccattg ccttagaggt
      601 attaatctat gctttataaa actataaggg aattgagata gttggctcat atacagaccc
      661 ctttcaagca ttagaagaat tgaaaattaa agatccagat gtgctctttt tagatataga
      721 aatggggcct ataaatggat tagaaatatc tgagtacttt ttacaagaaa aggagtcatt
      781 ggagataatc tttgttacag cctatcccca atatgcagtt ggggcctttg aaattgatgc
      841 catagattat ctattaaaac ctgtccaaaa aagtagatta gatatggcaa taaaaaggct
      901 taaagaaagg ctgagtacaa gtgcctactc atctagcaaa aagataaata gagatttaag
      961 aataaatagt tttggaagct ttcaagtatt agacagggag aataaacccc tagtctggag
     1021 gacgaaaaag ggaaaggagt tatttgccta tttatgggca caagagggac aaccggcgac
     1081 cagagatcag gttatggagc atgtattttc ccatatagac aataacaggg gaagtgctct
     1141 attgcataca actatctacc aaatacggaa gggcttaagc aacctgggtt atttagacgg
     1201 gattatatat accaatgatg gttataaatt aaacttgcca attaccagtg acttagatga
     1261 tttaactact atattaaaga aggaaaaata caatagggat gatataattg aaatactgga
     1321 aa
//
LOCUS       DATGSI010000265         1751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_396687, whole genome shotgun sequence.
ACCESSION   DATGSI010000265 DATGSI010000000
VERSION     DATGSI010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396687"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1751)
                     /locus_tag="VK031_04050"
     CDS             complement(<1..>1751)
                     /locus_tag="VK031_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HLS53120.1"
                     /translation="QVRLPNENTNPKLFNYRLSLKADNVKVTITTREHAISKLYKDRK
                     LKYFLKDKFVKYVENIFSSTLNEENSSLITSIILGKSSYMEEESLDFYREIGLAHVLA
                     VSGLHIGVISSFLIFLLSRLGIKRRTNVIITLSIIWLYGYMIDYPASILRANIMFTII
                     LYSQLIHRPFDSINNLALAAFILLVINPYYLFDVGFQLSFLATFSLLSFRPKIAHLFY
                     PYDNRLTNSLASILAVHIGLFPIQAYYFNSFNPLSLLANLVVVPLLSLSLIIGMMVLS
                     LYFWMGRFSLFFAYLLNLLLSLQFKLLRLLDSLPISLVKLKSPEMFSIILFYISLFII
                     FKMIKIDKIDKRIIRVIVVYLIILLVWDFFLIIRDESMEIHFIDVGQGDAILISTKYH
                     NYMMDTGGSLFGNFDIGKNITLPYLEKLGIRRLDAVFISHFHEDHCQALPTLMDQLRI
                     DNIVSGYAPEKGEVAEAIRESKIPFTIVNRGDRVYLDKNIVLEVLWPKEDVAYLSENN
                     KSLVALLRYRNFNILFTGDLEKEGEGILAKEMEEVDIIKIPHHGSKTSSTEGFLEKTR
                     PQYGVISVGRNNFYNHP"
BASE COUNT          598 a          342 c          260 g          551 t
ORIGIN      
        1 ctaggatgat tgtagaagtt gtttcgccct acagaaataa caccatactg gggcctggtc
       61 ttttctagaa atccttcagt agaagaggtc ttgctaccat gatgaggtat cttaattata
      121 tctacctcct ccatttcctt ggccaaaatc ccctcccctt ctttttcaag atctcctgta
      181 aacaaaatat taaaattcct ataccttaaa agagccacta aggatttatt attttcactt
      241 aagtaagcca catcttcctt tggccataaa acttccagca caatattttt atctaaatac
      301 accctatctc ccctatttac aatggtaaaa ggaatcttgc tttccctaat agcctccgcc
      361 acttctccct tttcaggagc atagccagat acaatattgt ctattcttaa ttggtccata
      421 agggttggca gggcttgaca atggtcctca tgaaagtggc ttataaaaac agcatctaat
      481 cttcttatgc ctaacttttc tagatagggt aaggttatgt tttttcctat atcaaaatta
      541 ccaaaaagac ttcctcctgt atccatcata taattgtggt atttggtact gattaatatg
      601 gcatcgccct ggcccacatc aataaaatga atctccattg attcatccct tataatcagg
      661 aaaaaatccc acacaagcag gataattaag taaacaacta ttacccttat tatcctttta
      721 tctattttat ctatctttat cattttaaat ataataaata gcgaaatata aaacaatata
      781 attgaaaaca tttctggcga tttaagcttg accaagctta tgggaaggct atctaaaagc
      841 cttagaagtt taaactgtaa actcaataaa agatttaaca aatatgcaaa aaataaacta
      901 aaccttccca tccaaaaata caggcttaaa accatcatac ctatgattaa agataaagat
      961 agcaagggca ccaccacaag gtttgccagc aaacttaagg gattaaagct gttaaagtaa
     1021 taggcctgga ttggaaatag tcctatatgg actgctaata tggaggccag gctatttgtc
     1081 agcctattat cgtaggggta aaataaatgg gctattttag gcctaaagct taacagtgaa
     1141 aatgtggcta aaaatgataa ttgaaagcct acatcaaaca aataataggg atttatcaca
     1201 agcaaaataa aggcagccaa ggctagatta ttaatggaat caaaaggcct atgaattagc
     1261 tgactataaa gaattatagt aaacattata ttggccctta gtatagaagc tggataatct
     1321 atcatgtatc cgtatagcca gattatagat agggttatta ttacatttgt tctccttttg
     1381 atacctaacc ttgataatag aaatattaaa aagctagaga taactccaat atgtaaacct
     1441 gacacagcca acacatgggc cagccctatc tccctataaa aatctaagct ctcttcttcc
     1501 atataggagg actttcccaa aattatacta gtaatcaagg agctgttttc ctcatttaat
     1561 gtgcttgaaa aaatattttc aacatacttt acaaatttat ccttcaagaa atacttaagt
     1621 tttctgtcct tatataactt tgaaatagca tgctccctgg tggtaatggt aacttttaca
     1681 ttgtcagcct ttagacttaa cctataatta aacagcttag gatttgtatt ttcattaggg
     1741 agcctcacct g
//
LOCUS       DATGSI010000266         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_633448, whole genome shotgun sequence.
ACCESSION   DATGSI010000266 DATGSI010000000
VERSION     DATGSI010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633448"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VK031_04055"
     CDS             <1..779
                     /locus_tag="VK031_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HLS53121.1"
                     /translation="QAVGTTGSGRHLAALIMGADIVKNEITCHAIAALDYNRQVKTVI
                     ETGGQDSKIIIIRDQVVVDFGMNTVCAAGTGSFLDRQALRLGIDISEFGGRALNSKSP
                     VRIAGRCAVFAESDMIHKQQLGHNQEDIIRGLCEALVRTYLSNVGKGKEILGPILFQG
                     GVAANQGIKEAFERELKEKVIIPPNYDVMGAIGAAIQGKEEAKSQGYTNFKGIDIYKS
                     DYEVNSIECNGCSNICEVLEIREREEVLARYGDKCGRWSF"
     gene            831..2033
                     /gene="thrC"
                     /locus_tag="VK031_04060"
     CDS             831..2033
                     /gene="thrC"
                     /locus_tag="VK031_04060"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HLS53122.1"
                     /translation="MEDLLICTNCKEGYNKETVDFRCQLCGEPLDVKEIKEGTIKEGN
                     PLKQSILDRYQDFYPYLSKANTLSLGEGFTPLVSSQSLAKEYGVSGLYFKDESSNPTW
                     SFKDRGTITGIIRAKDLGYFKIGTVSTGNMATSVAAYGARAGLETYILVKKDMAEEKL
                     KPISIYNPHLIRVDGDYGKLYYESLKLGHENQIYFINSDSPFRVEGYKTIAFEIAEQL
                     DFMPPDYLIVPTSAGGNFRGIEKGFRELKNVGIIDRLPKMVAVQATGCCPIYTAYKAG
                     KEKITRFERPKTIAQAIENPYPPSGNQVLRLIKDGRGLALSVTDEEIIRAQARLASIG
                     IFGQVASATSLAAVEKLRENNILKATDKVVAIITASGLKYTAALDKHSLKMETCKMED
                     LGQYIKSR"
     gene            complement(2061..>2325)
                     /locus_tag="VK031_04065"
     CDS             complement(2061..>2325)
                     /locus_tag="VK031_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53123.1"
                     /translation="SHHITANVIIALCASCEKKIIVPVQLCVLSTGFPELAPSVSPIC
                     STFPSLFPQQIDEGSLADSEGCGHQAYHSPAETPFSFSLHEEE"
BASE COUNT          785 a          395 c          558 g          587 t
ORIGIN      
        1 tacaggcagt aggcaccaca ggcagtggta ggcacctggc agccctaata atgggggctg
       61 acatagtaaa aaatgaaatc acctgccatg caatagctgc cctagactat aataggcagg
      121 tgaaaactgt aatagaaact ggaggacagg attcaaagat aatcataatc agggaccagg
      181 tggtggtaga ctttggtatg aatacagtat gtgctgcagg aacaggctcc tttttagaca
      241 ggcaggcctt aaggctagga atagatattt cagaatttgg aggcagggcc ctaaactcta
      301 aaagtccagt tagaatagca ggtagatgtg cagtgtttgc agaatcagac atgatacata
      361 agcaacagct aggccataac caggaagata ttataagagg cttatgtgaa gccttagtta
      421 ggacttattt aagcaatgtg ggtaaaggaa aagaaatttt aggccccatt ctttttcaag
      481 gaggggtggc agccaatcaa gggattaagg aggcctttga aagggaatta aaggaaaagg
      541 taataatccc gccaaactat gatgtaatgg gagctatagg ggccgccatc caaggtaaag
      601 aagaggccaa aagccaaggt tatacaaatt ttaagggaat tgatatatat aaaagcgatt
      661 atgaggtaaa ttccatagaa tgtaatggtt gttctaatat ctgtgaagtc ttagagataa
      721 gggaaaggga ggaagtttta gccaggtatg gagataaatg tggtagatgg tctttctaat
      781 ccttaagatc aatgatatag ttagattaaa agcaaataaa gggggatctt atggaagatt
      841 tacttatttg tactaactgt aaggaaggct ataataagga aactgtagac tttagatgtc
      901 agctatgtgg agagccctta gatgtaaagg agataaagga aggaaccata aaagagggta
      961 atcccttaaa acaatcaatc ctggataggt accaggactt ttacccctac ttaagtaagg
     1021 ctaatacctt atccctagga gaaggcttta cacctttagt tagctcccaa tccctggcca
     1081 aggagtatgg agtttccggc ctgtatttta aggatgaatc ctctaaccct acctggtcct
     1141 ttaaggatag gggaaccatt actggcataa ttcgagccaa ggatttaggt tactttaaaa
     1201 taggcactgt atctacagga aatatggcca catctgtagc tgcctatggg gccagggcag
     1261 gtttagaaac ctatatttta gttaaaaagg atatggcaga ggaaaagtta aagcctattt
     1321 ccatatataa tccccattta ataagagtag atggagatta tggaaaactt tattatgaaa
     1381 gcttaaagct aggccatgaa aatcaaattt actttattaa ttcagactcg cccttcaggg
     1441 tagaaggtta caaaactata gcctttgaaa tagcggaaca actggacttt atgccaccag
     1501 actatctaat agtacctacc agcgccggag gcaactttag gggaatagaa aaaggcttta
     1561 gggagcttaa aaatgtaggc ataatagaca gactacctaa aatggtggca gtccaagcta
     1621 caggctgttg cccaatttat actgcttaca aggcaggtaa ggaaaagata accagatttg
     1681 aaaggccaaa aaccattgcc caggcaatag aaaatcctta ccctcccagt ggcaaccagg
     1741 tcctgagatt aataaaagat ggtagaggtt tagccttatc tgtaacagat gaagaaataa
     1801 taagggcaca ggcaagatta gcctccatag gaatctttgg gcaggtagct tcagccacct
     1861 ctctggctgc agtagaaaaa ttaagggaaa ataatatatt aaaagccact gataaggtgg
     1921 tagctataat tacagcatca ggcttaaagt acacagccgc cttagataag catagtttaa
     1981 agatggagac ttgcaagatg gaggatttag gccaatatat aaaaagtagg tagcattaca
     2041 agctacctac tttttatcat ctactcttct tcatgaagag agaagctaaa gggagtttct
     2101 gctgggctat gataggcctg atggccacat ccttctgaat ctgctaggct tccttcatca
     2161 atttgctgcg ggaataggct tgggaaagta gagcatattg gggaaacgga aggtgcaagc
     2221 tctgggaatc cagtagagag gacgcatagt tgcacaggaa ctatgatctt cttctcacag
     2281 ctggcacaca gggcaatgat tacatttgct gttatgtggt gggag
//
LOCUS       DATGSI010000267         1851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_161962, whole genome shotgun sequence.
ACCESSION   DATGSI010000267 DATGSI010000000
VERSION     DATGSI010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161962"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1170)
                     /gene="hemZ"
                     /locus_tag="VK031_04070"
     CDS             complement(193..1170)
                     /gene="hemZ"
                     /locus_tag="VK031_04070"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HLS53124.1"
                     /translation="MYPLDQNRYSLYIGIPFCPTRCSYCSFPSYALAGKTYKAQPYID
                     TLIYEINSIKDLMAGKKINTVYIGGGTPTAVSRKQLESVIESVQTGFNGEIKEFTVEA
                     GRPDTIDRDMLKMLKDKGVTRISINPQTMNGDTLKLIGRNHSPKSIIRAYNLAKSVGF
                     DIINMDIILGLPGETPEDLQYTLERIEELDPENLTIHILSLKRGSKLYNAGYKHIGKV
                     NAEIEEMLDLTKEFTYKMNLLPYYLYRQKQILGNLENVGYAKRGMECVYNIAMMEEKE
                     TVIGLGLGSVSKIFYPKENRIQRVPNFKGLDDYIRRVDELIARKKHYII"
     gene            complement(1158..1655)
                     /locus_tag="VK031_04075"
     CDS             complement(1158..1655)
                     /locus_tag="VK031_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53125.1"
                     /translation="MIQVYLEGHAYKHDLFELIRVFFPGRQINFVEKEKRGKCKYLIE
                     SILTEKSGKFFKITNLYKDSQLIKTIREEINDKMYKGLDMESLIKLRIKRGVYLILSS
                     SLDIDLPWGILTGIRPVKLVHDFYEKEMENKIRYILEKEYKLDETRVELLLQVAAIQN
                     NTFTL"
BASE COUNT          530 a          366 c          241 g          714 t
ORIGIN      
        1 aggctctgct actccgggag tgtaagctat acttaggtca tccttagttt caaccttgag
       61 ctttgatatc acttcaattt tcccctggtt ttcctcatga gcctttaaac ttaattctgt
      121 gtaatccata aagtttccac cctttctttt ggttaaaata tgttttcacc aaatacatat
      181 aatatattta gatcaaatta tataatgctt ctttctggct ataagctcgt ctactctcct
      241 tatataatca tctaagcctt taaagtttgg gacacgttgg attctgtttt cctttggata
      301 aaaaatttta gatactgagc ctagtcctag accaataact gtttcttttt cttccatcat
      361 agctatatta tatacgcact ccatacccct tttagcatag ccaacgtttt caaggttgcc
      421 taaaatttgt ttctgcctgt ataaataata gggtagcaga ttcattttat atgtaaattc
      481 cttggttaaa tctaacattt cttcaatctc tgcatttact ttaccaatat gtttataacc
      541 agcattgtaa agcttggatc ctctttttaa agataatata tggattgtta gattttcagg
      601 atctaattct tctattcttt ccaatgtata ttgtaaatct tcaggggtct cacctggtaa
      661 ccccaatatt atatccatat ttattatatc aaagcctaca cttttggcaa ggttataggc
      721 tctaataatc gattttggac tatgattcct accaataagt tttaatgtat ctccattcat
      781 ggtttgagga ttaatactaa ttctagtaac tcccttatcc ttcagcatct tcagcatatc
      841 acgatcaatt gtatccggcc ttcccgcctc tacagtaaat tctttaattt ctccgttaaa
      901 cccagtttgt acagattcta taacactttc caattgtttt cgagatactg cagttggagt
      961 ccctcctcct atgtagacag tgtttatctt tttacccgcc atcaaatcct taatactatt
     1021 tatttcataa attagtgtat ctatataagg ttgggcctta tatgtctttc ctgctaatgc
     1081 ataggaggga aatgaacaat atgagcatct ggttggacaa aatgggatgc ctatataaag
     1141 gctataccta ttctggtcta aagggtaaat gtgttgtttt gaattgctgc cacttgtaag
     1201 agtagttcaa cccttgtttc atctaattta tattcttttt ctaaaatata tcttatttta
     1261 ttttccattt ccttttcata aaaatcatga accaatttaa caggccttat accagttaat
     1321 attccccaag gcaagtctat atctaatgaa gaactaagga ttaaatacac tcccctttta
     1381 attcttagct ttattaaaga ctccatatct agccccttgt acattttatc attaatttct
     1441 tccctgatag tttttataag ttgggaatct ttataaagat tagttatttt aaaaaatttt
     1501 cctgactttt ctgttaaaat actctcaatt aaatacttac attttcctct tttctccttt
     1561 tctacaaagt ttatctgcct acctgggaaa aaaactctaa tcaattcaaa taaatcatgt
     1621 ttataagcat gtccttctag atatacctgt atcattctat caccacctat taaaaggcca
     1681 cctttctaaa tagaaaagta gcctcttctt aatttaaata tggattatat agtttttctt
     1741 cattaatggt agacgcatct ccgtggcctg gccaaacttt catatgccca tccaggacta
     1801 gtagcttgtt ttttatggag ttaattaagg taacatgatc tcctccatat a
//
LOCUS       DATGSI010000268         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_161969, whole genome shotgun sequence.
ACCESSION   DATGSI010000268 DATGSI010000000
VERSION     DATGSI010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161969"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VK031_04080"
     CDS             <1..266
                     /locus_tag="VK031_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLS53126.1"
                     /translation="LDGVLEEIDKKIGLDRLKAVHLNDSKVEFASNKDRHEVIGGGTI
                     GLKAIVNMINHPLLKDLPFNLETPNELEGYKEEIELLRKEYIW"
     gene            260..1285
                     /gene="queG"
                     /locus_tag="VK031_04085"
     CDS             260..1285
                     /gene="queG"
                     /locus_tag="VK031_04085"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HLS53127.1"
                     /translation="MVNIRDYIEKRAQDIGIDIIGFTDGKPLSRIKPYLEFRIENRLI
                     TEFEEANISKRIDPKTTLPNCKSIIVIGISYNVDFKIKRGEKPVGILSKSSWGLDYHK
                     LLKEKMEKLIEEIKKKVDFKYKAYVDTGPLIDRELAHKAGIGYYGKNCSIINKEYGSF
                     IFLGYILTDLGIKTYSIRLESECGSCDLCIRACPTGALEGSYRLNPKKCISYLTQTRE
                     VIEEGLRGKMANKIYGCDTCQQVCPKNKGVKLSRHKEFMPNVTKGVVDLGELFSMSNR
                     EFRQKYGHMAGSWRGRNLLRRNGIINLVNMKEDLSLIEELDQENPLYKNYIDWARKKT
                     SKANWNK"
     gene            1345..2532
                     /locus_tag="VK031_04090"
     CDS             1345..2532
                     /locus_tag="VK031_04090"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HLS53128.1"
                     /translation="MSSVHDLDFLKEKIKQLKDEGVYRQLPVLETANEAEVVLNGRSV
                     INLSSNNYLGFSNHPRLKKAAIEAVEKYGVGAGAVRTIIGNMDIHEELESLLADFKRE
                     EAAFIYQSGFNCNAGTIQAITDRGDLIISDELNHASIIDGARLSRADKAIYKHSDMDS
                     LEKVLKENRDKYKNMLIITDGVFSMDGDIANLPEIVELAEKYEAMTYVDDAHGSGVLG
                     ESGRGTVDHFNLHGRVDFVMGTLSKAIGVIGGYVAGSQTMYDWLNHRARPVLFSTSLP
                     PAVIGSITEAIKMLMESTEYTDRLWDKARYFKERLAKLGYDTGNSQTPITPVIIGDEA
                     KTMDFSKKLLEEGVFASGIVFPTVPKGTARVRCMVTAAHSKEQLDRAIEVFEDVGKKM
                     NII"
     gene            2656..2946
                     /locus_tag="VK031_04095"
     CDS             2656..2946
                     /locus_tag="VK031_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HLS53129.1"
                     /translation="MSILIIGTCSLKLMIYGSQSLKDKRHTVKSIIGRLKSRFNISIS
                     EIDLNDNWQMAEIGLACVTNDTGHANEIISKVIKFIDNDSRVEILDYNIEIL"
BASE COUNT         1149 a          428 c          702 g          839 t
ORIGIN      
        1 atctggacgg agtattagaa gaaatagata aaaagattgg gcttgatagg cttaaagcag
       61 tccacttaaa tgatagcaag gttgaatttg cctccaataa ggatagacat gaagttatag
      121 gaggagggac cataggattg aaggcgattg taaatatgat aaaccatccc ttacttaaag
      181 atttaccctt caatctggaa actcccaatg aattagaggg ctacaaggaa gagatagaat
      241 tattaagaaa ggaatatata tggtaaatat tagagactat attgaaaaaa gggctcaaga
      301 tataggtata gatataatag gctttactga tggaaagcct ctctctagga taaagccata
      361 cttagaattt aggatagaaa ataggttaat aacagaattt gaggaggcga atataagtaa
      421 aaggattgat cctaagacta ccttacctaa ctgtaaaagc ataatagtaa taggtatctc
      481 ctataatgtg gatttcaaaa ttaaaagggg ggaaaagcct gtaggaattt tatctaaatc
      541 ctcctgggga cttgattacc ataagctttt aaaagaaaaa atggaaaaat taatagaaga
      601 aataaaaaag aaggtagatt tcaaatataa ggcttatgta gacacagggc ccctaataga
      661 tagagaattg gcccataagg caggcatagg atattatgga aaaaactgct ccataataaa
      721 caaagaatac ggctccttca tttttttagg ctatatctta acagatttgg gtataaagac
      781 ctactccatt agattagaat cagagtgtgg atcctgtgat ttatgtatca gggcttgtcc
      841 cacaggggcc ttggaaggtt cttataggtt aaatcctaaa aaatgcatat cttacctgac
      901 ccagaccagg gaggtaatag aggaaggcct aagagggaaa atggccaaca aaatatatgg
      961 ttgtgatact tgccagcagg tttgtccaaa aaacaagggg gtaaaactaa gtagacataa
     1021 ggaatttatg cccaatgtaa ctaagggagt tgtggattta ggagagcttt tttccatgtc
     1081 caacagagaa tttaggcaga aatatgggca tatggcaggt agttggagag gaaggaacct
     1141 gctaagaaga aatggaatta taaatctggt taacatgaaa gaagacttaa gcttaataga
     1201 ggaattagat caggagaatc cgctttataa gaattatata gattgggcca ggaagaaaac
     1261 ttcaaaggcc aactggaata aatagccata agggaatagc aattgtgata taatatattt
     1321 aataaataac tgggagatga tcgcatgtct agtgtccatg atttagactt tttaaaagaa
     1381 aaaataaaac agttgaagga tgagggagta tacagacagt tgcctgtttt agaaactgcc
     1441 aatgaggctg aggtagttct aaacggtaga tcagtaataa acctttcttc caacaactac
     1501 ttaggttttt ctaatcaccc tagattaaaa aaggcagcca ttgaggctgt ggaaaaatat
     1561 ggagttggag ctggggctgt aagaaccata ataggcaata tggatatcca tgaagagttg
     1621 gaaagtttat tggcagattt caaaagagag gaagcagcat ttatttacca atctggtttt
     1681 aactgtaatg ctggaactat tcaggccata acagataggg gagaccttat aatatctgat
     1741 gaattaaacc atgcctccat aatagatggg gccagattat ctagggctga taaggctatt
     1801 tacaaacatt ctgatatgga tagcttagaa aaggttttaa aggaaaatag ggataagtat
     1861 aaaaatatgc taataattac tgacggtgta tttagtatgg atggggatat tgccaactta
     1921 ccggaaatag tagagttggc tgaaaaatat gaggccatga cctatgtaga tgatgcccat
     1981 gggtcaggtg ttttaggcga aagtggccga ggtacggtgg atcattttaa cctacatggg
     2041 cgggtagact tcgttatggg taccttatct aaggccatag gtgtaatagg tggatatgtg
     2101 gccgggtcac agaccatgta tgattggcta aatcataggg ctaggccagt attattcagc
     2161 acatccttgc caccggcagt tattggatca ataacagaag ctataaagat gttgatggag
     2221 tctactgaat acacagatag gctatgggac aaggctagat attttaaaga gaggctagct
     2281 aagctaggct atgatacagg gaatagccaa acacctataa ctcctgtaat cataggagat
     2341 gaggctaaga ccatggactt tagcaaaaag ttattagaag agggagtatt tgcttcagga
     2401 atagtctttc caacagttcc caagggaaca gccagagtta gatgtatggt aactgcagcc
     2461 catagcaaag aacagctaga tagggctatt gaagtatttg aagatgtagg aaagaaaatg
     2521 aatataattt aggacaaaaa gggatccttt caagaagtat ttaaagggcc gttcttttag
     2581 ataaatatct tgagaaggat ctctaatttt ttagaaaata tatggaacaa attgccatat
     2641 actatataat atgaggtgag cattttgata ataggaacct gctctttgaa attgatgata
     2701 tatggttccc aatctctaaa ggataagaga catacagtca aaagtataat aggaagatta
     2761 aaatcaaggt ttaatatttc tatttcagaa atagatttaa atgacaactg gcagatggca
     2821 gaaataggtt tggcctgtgt aaccaatgac actggccatg ccaacgaaat tatatcaaaa
     2881 gtaataaaat ttattgataa tgatagccgg gtggaaatcc tggattacaa tatagaaata
     2941 ttataattat ctataacaaa gaaggtaggg aagttatatg aaaaagaagg tgctattaat
     3001 cctaatcccc ttaatcgtac tattaagtgg ggtggccagt gccagctatt atatttacaa
     3061 taatacagtt aatgtggaca caatatacac aggaatacaa atagatgaaa aaaatgtg
//
LOCUS       DATGSI010000269         1305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_161974, whole genome shotgun sequence.
ACCESSION   DATGSI010000269 DATGSI010000000
VERSION     DATGSI010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161974"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="VK031_04100"
     CDS             complement(<1..160)
                     /locus_tag="VK031_04100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLS53130.1"
                     /translation="MKYLISGGGTGGHIYPALAIAREIERRDQEAKILYVGTKTGLEA
                     KLVPREGLD"
     gene            complement(162..1286)
                     /gene="ftsW"
                     /locus_tag="VK031_04105"
     CDS             complement(162..1286)
                     /gene="ftsW"
                     /locus_tag="VK031_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HLS53131.1"
                     /translation="MARNKKTKRRAVDFTLLLTIIILVFIGIIMVFSASWPEAMAKHN
                     NDGYYFLKRQLSYAILGFFGLIIMMNFDYRHLKRLAPLIYILSIISGLLLFTSLGKEL
                     KGATRWLDLKIITIMPSDIIKVGSIIFFASFLSNKREKITSLVHGTIPALIYIAISCG
                     IIVTQDLGTAVTLAGTMFAMFFVAGMKFTHLIPLGLGAGGLLWYAIHDSDHPHRIKRL
                     TAFRDPFADKLGDGWQVVQSLYALGSGGLFGLGLGKSRQKFFYISEAYNDFIFAIIGE
                     ELGLLGTLLVLILFMIVIWRGIIIALKVEDVFACFLATGITALIAVQSMIHIAVVTSS
                     IPTTGITLPFISYGGTSLVIYMAAVGILLNISRHANLGRN"
BASE COUNT          433 a          275 c          206 g          391 t
ORIGIN      
        1 aatctaggcc ctccctaggt actagctttg cttctaagcc tgttttagta cctacataga
       61 ggatttttgc ctcctgatcc cttctttcta tttctctggc aatggccaag gctggatata
      121 tatgcccccc tgtcccacca ccagatatta aatacttcat attaattcct cccaagattt
      181 gcgtgacgtg atatgttaag taatatgccg actgctgcca tatatataac taaagatgta
      241 cctccataac ttataaaggg taaggtaatt cctgtggtag gaattgagga tgtgaccact
      301 gctatatgga tcatagattg gacggctatt aaagcagtta taccagtggc taaaaaacag
      361 gcaaaaacat cttcaacctt caaggcaata attatacccc tccagattac aatcataaat
      421 aatataagta ctaagagggt acctaataaa cctaattctt cacctattat tgcaaagata
      481 aagtcgttat aagcctctga tatatagaaa aacttctgcc tgcttttacc taggcctaat
      541 ccaaataaac ctccagagcc taaagcatat aaagattgaa ccacctgcca gccatcacct
      601 aatttatcag caaaaggatc tctaaaagct gtaagtcttt taatcctatg gggatgatca
      661 gaatcgtgaa tagcatacca tagtagtccg cctgccccca atcccaaagg aatcaggtgg
      721 gtaaatttca tccctgcaac aaaaaacatg gcaaacatgg tcccggcaag ggtaacagct
      781 gttcctaaat cctgagttac aataatccca caggagatag ctatataaat tagggccggt
      841 atggtgccgt gaaccaggct tgttattttt tcccttttat tagataaaaa ggatgcaaag
      901 aatatgatag aacctacttt tataatatct gatggcatta tggttataat ctttaaatct
      961 aaccatctgg tggcaccctt taattcctta cctagactag taaataaaag caatccagat
     1021 ataatagata aaatatatat taaaggtgcc agccttttta gatgtctata gtcaaaattc
     1081 atcataatta taagtccaaa gaaacctaat atagcataac taagttgcct ttttaaaaaa
     1141 taatatccat cattattatg ttttgccatg gcctctggcc agctggcact aaagaccatt
     1201 ataatcccta taaaaactag aataattatt gttaacaaca aggtgaaatc tacagctctt
     1261 ctcttggtct ttttatttcg agccatctta ttcctccctt aaaag
//
LOCUS       DATGSI010000270         1688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_396841, whole genome shotgun sequence.
ACCESSION   DATGSI010000270 DATGSI010000000
VERSION     DATGSI010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396841"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VK031_04110"
     CDS             <1..321
                     /locus_tag="VK031_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HLS53132.1"
                     /translation="LYNILSAMLEDIMKRGKTEVGSKTMVDALAPAVEEYKAQMDKEA
                     DDKELLEAVAQAAKEGAEATRDMEAIRGRASYQANKGVGHLDPGAVTMAYQIEILCNY
                     ILQL"
     gene            332..1351
                     /locus_tag="VK031_04115"
     CDS             332..1351
                     /locus_tag="VK031_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HLS53133.1"
                     /translation="MSINKRELLRKAFDNEEVDRVPVGFWHHFVLGEDQFIGLEDKSI
                     LDKVIQGHVDYFNTTNPDMMKLMNEGFMGYPPIMGNKFETAEDLKKVKSIGPDHPWIT
                     EQIKHVKKLVSLFGDKVMTFYNVFAPLQVMRIRFDFLDLDFDRFVYLAENFPEEFHQA
                     GMEIQKDIKILVEKLLTETDLDGIYYCVQNIQSEKYDLETYNKYIRPTEIEVLDVANK
                     HSDYNILHICGYANYKNDLSFYKDYEAKVYNWATFTEEVSIKEGREIFKDKAILGGFD
                     NNPGTLIDTGSPEELEAFVKELIDENGYKGFIIGADCSIPNDIDDRQVRYISDASHKF
                     IKGAE"
     gene            1353..>1688
                     /locus_tag="VK031_04120"
     CDS             1353..>1688
                     /locus_tag="VK031_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53134.1"
                     /translation="MDRRQVMDAFLDNKTPDRIPSAFWYHFVSFHDHYNAHIPEVYET
                     VITEQKRYIDEVRPDLIKIMSDGFFGHPSVTRKLIETVDDIKGIESVGQDHQWMKHQI
                     EYVKEVCDHA"
BASE COUNT          629 a          246 c          376 g          437 t
ORIGIN      
        1 ctttataata ttttatcagc catgttggaa gacataatga aaagaggaaa gacagaggta
       61 ggatcaaaaa ccatggtaga tgctttagcc cctgcagtgg aggaatataa agcgcagatg
      121 gataaggaag cagatgataa agaattactg gaggctgtgg cccaggcagc caaagaggga
      181 gcagaagcta ccagggacat ggaggctatc agaggaaggg ccagctatca ggctaacaaa
      241 ggtgtagggc atctagatcc tggtgcagtg accatggctt atcaaataga aatattatgt
      301 aactatatct tacaattata ggagagattt tatgagcata aacaagaggg aacttttgag
      361 aaaagcattt gacaatgagg aagtagatag agtaccagtt ggattttggc atcactttgt
      421 cctaggtgaa gaccaattta tagggttgga agacaaatca atcctggata aggttatcca
      481 aggacatgtg gattacttta atacaactaa tccagacatg atgaaactga tgaatgaagg
      541 atttatgggt taccctccta ttatgggaaa taaatttgaa acagcagagg atttaaaaaa
      601 agtaaaaagc ataggaccag atcacccttg gattacagaa cagattaaac atgtaaaaaa
      661 gttagtcagc ctatttgggg acaaggtaat gaccttctat aatgtatttg ccccgcttca
      721 ggtaatgaga attaggtttg actttttaga cctagacttt gataggtttg tgtatctggc
      781 agaaaacttc ccagaggagt tccaccaggc aggtatggag attcaaaaag acataaaaat
      841 cctagtagaa aaactattaa ctgaaacaga tctagatgga atctactact gtgtacaaaa
      901 cattcaaagt gaaaagtatg atttagaaac ttataataaa tatataagac ctacagaaat
      961 agaagtatta gatgtggcta acaaacatag cgattataat attcttcaca tctgtggcta
     1021 tgccaattac aaaaatgatc taagcttcta taaggattat gaggctaaag tttataactg
     1081 ggccaccttt acagaagaag taagcataaa agaaggcagg gaaatcttta aagataaggc
     1141 aatactggga ggatttgaca ataatccagg caccttaata gatacaggta gtccagagga
     1201 gctagaagcc tttgttaagg aattaataga tgaaaatggt tacaaagggt ttattattgg
     1261 ggcagactgt tccataccca acgacataga tgataggcag gttagatata tttctgatgc
     1321 cagtcacaaa tttataaagg gagctgaata aaatggatag aagacaggta atggatgcat
     1381 ttttagataa taaaacacca gataggattc caagtgcttt ttggtatcac tttgtttcct
     1441 tccatgatca ctataatgcc catattccag aggtatatga aacagtgatt acagaacaaa
     1501 aaagatatat agatgaggta aggccagatc tgattaagat tatgtcagat ggcttctttg
     1561 gacacccttc agttacaagg aaattaatag aaacagtaga tgacataaaa ggaatcgaaa
     1621 gtgtaggaca ggatcatcag tggatgaaac accaaattga atatgtaaaa gaagtttgtg
     1681 accatgcg
//
LOCUS       DATGSI010000271         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_115026, whole genome shotgun sequence.
ACCESSION   DATGSI010000271 DATGSI010000000
VERSION     DATGSI010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115026"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..795)
                     /locus_tag="VK031_04125"
     CDS             complement(43..795)
                     /locus_tag="VK031_04125"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53135.1"
                     /translation="MDIKIVSYNPGSKSAKKLAEALGVFRLRHKGRRIVLNRNRLINW
                     GCSELPERIVPNNYVYNKPEHIRKASDKLQAFFALSPSVPMPEWTIDQQEALEWLREG
                     SMVVCRTKLRGHSADGLVLADKEEDLVYAPLYTKYEKKIHEYRVHVFQGEVIFVQRKA
                     RKREVPDEEVNWQVRNMKNGFIFANKDVEIPKNVQESCVQAVDILCLDFGAVDVLVNK
                     DNEHFIIEVNTAPGLAGTTLEKYTEAFRRTLL"
     gene            complement(785..1729)
                     /locus_tag="VK031_04130"
     CDS             complement(785..1729)
                     /locus_tag="VK031_04130"
                     /inference="COORDINATES: protein motif:HMM:NF023645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HLS53136.1"
                     /translation="MILRDLLGKNRAPTGDLGLEIEMEGILLPPSVDGWNYVPDGSLR
                     DGGVEYVLSKPESLEDTKRLISELTEALLDNGKNPVFSFRTSVHVHVNCMRMTYHQIL
                     NYILLYYLLEQGLTEYCGEGRKANRFCLRLFDAEGIVFNAEQLFRTSRPQRAIASLSA
                     DASKYAALNLSSLRTFGSLEFRSMRGTLDPDVLFPWIETLLHMREYSLKFKVPMEILD
                     RIDEIGEEEFIKEALGDNYSLFIDRSLQEIALSRSLTVDLPIQVKKEYKEKELPLELE
                     VDIGFGPITIQEIIDDYEGNVPPVIMRAIDNYLEENGY"
     gene            complement(1719..>2284)
                     /locus_tag="VK031_04135"
     CDS             complement(1719..>2284)
                     /locus_tag="VK031_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53137.1"
                     /translation="TTMYSRSVVLYQGEPIYIRYITGALMCIYTEMSTGYIKATRWAQ
                     DKFKNPAIRLGYINLGQGCAYLYRKPVRQYRVGLSSENTSVRSVDMGLSDHDKDLLQS
                     IRRDWNCKNIYDTLTGVYPSFEDAYREAMDIEGARAFDRQFAVDWGGNIYYKSELVGN
                     AEDGVIVFLDSKDYLRNNLPEGVRNDS"
BASE COUNT          648 a          508 c          361 g          767 t
ORIGIN      
        1 tatatttctt agccttctta ggggtagtgg catggaataa tctcacagta aggttctcct
       61 aaaagcctca gtgtattttt ctaaagtagt tcctgctaat ccaggggcag tgttcacctc
      121 gattataaaa tgttcattat ctttattaac aagtacatct acagccccaa agtcaagaca
      181 taatatatct actgcttgaa cacagctttc ttgaacattt ttaggaattt ccacatcttt
      241 attggcaaat ataaatccat tcttcatatt tctcacttgc caatttactt cttcgtctgg
      301 aacctctctt tttctagcct tacgttgaac aaagataacc tctccttgaa acacatgaac
      361 tctatattca tgaatctttt tttcatattt tgtatacaaa ggtgcgtata ctaaatcctc
      421 ttctttatct gctaaaacca acccatcggc cgaatgtcct ctgagtttag ttcggcaaac
      481 aaccatacta ccttctctaa gccattctaa ggcctcctgt tggtcgatcg tccattcagg
      541 cataggaaca ctaggggata gggcaaaaaa cgcttgtagc ttgtctgaag ccttcctgat
      601 atgctctggc ttattataaa cataattgtt aggaacaatt ctctcaggca gttcggagca
      661 tccccaatta ataagtctat ttctattcaa tacaatcctg cgtcctttat gcctgagtct
      721 aaatactcca agagcttccg ccagtttctt ggccgactta cttccagggt tgtatgaaac
      781 aattttaata tccattttct tccagataat tatcaattgc cctcatgatt acaggaggaa
      841 catttccctc gtaatcatct ataatttctt ggatggttat agggccaaat cctatatcaa
      901 cttctaattc taaaggtaat tccttttcct tatattcctt ttttacttga atgggcaaat
      961 ctacagttaa ggaacgagac agagcgattt cttgtagcga ccgatcaata aataaactgt
     1021 agttgtcccc caaagcttct ttgataaatt cttcttctcc tatttcatca attctgtcta
     1081 agatttccat aggaaccttg aatttaagag aatactctct catatgtaac agtgtttcta
     1141 tccaaggaaa caatacatca ggatcaagag ttcccctcat agacctgaac tctagagaac
     1201 caaaagttcg aagagaactt aggtttaacg cagcatactt agaagcgtct gctgacaaag
     1261 aggcaatagc tctttgaggt ctagaggttc taaacaactg ttctgcattg aatactattc
     1321 cttctgcatc aaataatctt aggcagaacc tgttagcttt tctgccttct ccgcaatatt
     1381 ctgttaaacc ttgctctaat agatagtata agagaatata gtttaagatc tgatggtaag
     1441 tcatcctcat acagtttaca tggacgtgta cactggttcg gaaagagaat acagggtttt
     1501 tgccgttatc tagcaatgct tcagtcagtt cgcttattaa tctcttagtg tcttctaggc
     1561 tctctggttt agataataca tactctaccc ctccgtccct tagagaacca tctggtacgt
     1621 aattccaacc atctactgaa ggagggagaa gaatcccttc catttctatt tctaatccca
     1681 ggtctcctgt gggggctctg ttttttccta gtaaatctct aagaatcatt tctaactcct
     1741 tctggaagat tatttctaag atagtcttta ctatctaaaa atactattac tccgtcctcc
     1801 gcgttcccta ctaactcaga cttgtaatag atgtttcctc cccaatccac agcaaactgc
     1861 ctatcaaaag ccctggctcc ctctatgtcc atggcctctc tataagcatc ttcaaatgaa
     1921 ggataaactc ctgttaaggt atcgtatatg tttttacagt tccaatctct tctaatagat
     1981 tgtaataaat ctttatcgtg gtcggacagt cccatatcta ctgacctaac agaggtattt
     2041 tcagaagaca atcctactcg atattgccta actggttttc tatatagata agcacaacct
     2101 tgtcctaagt ttatatatcc caatcttatt gctgggttct taaacttatc ttgagcccat
     2161 ctggtagctt tgatgtatcc tgtagacatt tctgtataga tgcacataag agcgcctgta
     2221 atatatctaa tatagattgg ctctccttgg tataaaacaa cgctacggga atacatagtt
     2281 gtaa
//
LOCUS       DATGSI010000272         1146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_17574, whole genome shotgun sequence.
ACCESSION   DATGSI010000272 DATGSI010000000
VERSION     DATGSI010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17574"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1146
                     /locus_tag="VK031_04140"
     CDS             <1..>1146
                     /locus_tag="VK031_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53138.1"
                     /translation="FLGQPTQEYISDLNQFISDYNKFDSFKDVLYPIPRMSNNDTTTS
                     LLQMVGALYGRTLSQSEIDSLGSYVDLSLLKTENPYSNLTPLKDLGFLGVFNKKSPAS
                     DVFSQETFSEEIEDLLSLDSFYSTYEEIPVEKQKKKVVAYKEISTDNKEYFDNYSFTL
                     SYSFLLNSWVSYHSYLPLYYIEANNHFYSGVNDEIWEHTNNIKYNNFYGKIFPYIIEF
                     PYFYKYNDEILQYIEDYTKVYKPLSKSDNDFIKVEDKYFDKAVIFNDVQNSGYLNLKK
                     KPDHDMSAYLTYPKYNNDSKDILYTLSNTFYKYNTFWNALKDPNMPQFKKGLTLNIPE
                     KLDNSNIDYTQRDFKKESIRSKDIKIRHILDSTDEYKLVSQFLLTQT"
BASE COUNT          428 a          158 c          170 g          390 t
ORIGIN      
        1 gttttttagg gcagcctact caagaatata tttctgatct aaatcaattt atatctgatt
       61 ataataagtt tgacagtttt aaggatgtat tgtatcctat acctaggatg agcaataatg
      121 acactaccac ctctctacta caaatggttg gtgcgttata tgggagaact ttatcacaat
      181 cagagataga ttcattagga agttacgtag atttatcatt gttgaaaaca gaaaatccct
      241 atagtaactt aacaccttta aaagacttag gatttcttgg ggtattcaat aaaaaatctc
      301 ctgcatcaga tgtattttct caagaaactt tctctgaaga aatagaagat ttattatctt
      361 tagattcttt ttattctact tatgaggaga taccagttga aaaacaaaaa aagaaggtag
      421 ttgcttataa agagataagt acggacaata aagaatactt tgataattat tcttttactc
      481 tgtcttattc tttcttatta aattcttggg tatcttatca tagttactta cctttatact
      541 atatagaagc taataatcat ttttatagtg gagttaatga tgaaatatgg gagcatacca
      601 ataatattaa gtataataac ttctatggca aaatcttccc ttatataata gagttcccat
      661 atttttataa atataatgat gaaatattac agtatattga ggattatacc aaagtgtata
      721 aaccattaag caaatcagat aatgatttta taaaagtaga ggataagtac tttgataaag
      781 ctgtcatatt taatgatgta caaaattctg gctacttaaa cctaaagaag aagccagatc
      841 atgatatgag tgcctatctt acttatccta agtataataa tgacagtaag gatatccttt
      901 acactttatc taataccttc tacaaataca atactttttg gaacgcattg aaggatccaa
      961 atatgcctca atttaagaaa gggttgactt taaatattcc agagaaatta gataatagca
     1021 atatagatta tactcagagg gatttcaaaa aagagtctat aagatctaaa gatataaaga
     1081 ttaggcatat cttagatagt acagatgagt ataaactagt atctcagttc ctactaacac
     1141 agacta
//
LOCUS       DATGSI010000273         1851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_17590, whole genome shotgun sequence.
ACCESSION   DATGSI010000273 DATGSI010000000
VERSION     DATGSI010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17590"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /gene="metG"
                     /locus_tag="VK031_04145"
     CDS             <1..1471
                     /gene="metG"
                     /locus_tag="VK031_04145"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HLS53139.1"
                     /translation="FFRLSKYRDRLLKLYEDNPNFIQPESRKNEMINNFLKEGLEDLC
                     VSRTSFDWGIEVPFDPEHVIYVWVDALSCYLTALGYGSEDDENYKKFWPANVHLVGKE
                     IVRFHTIIWPALLMALDIELPEQVFGHGWILFDDDKMSKSKGNIIYPEPIIELYGIDA
                     YKYFLLREFSFGQDGSFTREKFLQRLNSDLANDLGNLISRTVTMVEKYNEGVIPAVSK
                     EEEIDDSLRKLAESTADKVEAHMDRLDFSHGLEEIWKLVRRTNKYVDETMPWILAREE
                     NKERLDTVLYNLSESIRIISILIKPFMETTSHEIRRQLGIEDEIKWEDTKQWGLLKSG
                     SKVNKGDIIFPRLDIEKELVRLDEANEKLIELRTKNRVKEDKKVEKDQLITIDDFSKV
                     KMKLAEVIKCEDHPQADKLLVLTLKLGEETRQVVSGIKEFYQAEDLVGKKVVLVTNLK
                     PVKLRGIESQGMILAAEDSKGDLSLLTTLEDLQDGATVS"
     gene            1481..>1851
                     /locus_tag="VK031_04150"
     CDS             1481..>1851
                     /locus_tag="VK031_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HLS53140.1"
                     /translation="MLIDSHVHLDDKRFDKDRHELIQSLKDNGVELVINIGADLRSSI
                     NSVRLAEKYENIYATVGVHPHSAKDVDTETIKKLKELTKEKKVVAIGEIGLDFYYDNS
                     PRELQRKWFKEQLKLAKEVDLP"
BASE COUNT          688 a          247 c          417 g          499 t
ORIGIN      
        1 cttttttagg ctttctaagt atagggatag gctactaaag ctatatgaag ataatcctaa
       61 ctttatccaa ccagaatcca ggaaaaatga aatgattaat aacttcttaa aggaaggctt
      121 agaagattta tgtgtgtcta gaactagttt tgactgggga attgaagtac cctttgatcc
      181 agaacatgta atatatgttt gggtagatgc cctatcctgt tatttaacgg ccttaggata
      241 tggcagtgaa gatgatgaaa attataaaaa gttttggcca gccaatgtac atctggtagg
      301 gaaagaaatt gtaagattcc atacaattat ttggccagca cttttgatgg ctctagatat
      361 agaattgcct gagcaagtat ttggtcatgg ttggattctg tttgacgatg ataaaatgtc
      421 taaatctaag ggaaatatta tttatccaga accaataata gagttatatg gaatagatgc
      481 ctataaatat ttccttttaa gggagttttc ctttgggcag gatggttctt ttaccaggga
      541 gaagttttta caaagattaa attcagacct ggccaatgat ttaggtaacc ttataagtag
      601 gactgttacc atggtggaga aatataatga aggagttata ccggctgtct ccaaggaaga
      661 ggaaatagat gactccttaa ggaaattggc agaatctaca gcagataagg tagaggccca
      721 tatggatagg ttagactttt cccatggctt ggaagagatc tggaaattgg ttagaagaac
      781 caataagtat gtggatgaaa ccatgccctg gatcctggcc agggaagaaa acaaggaaag
      841 attagacacg gttttatata atttatctga atctattagg atcatctcaa ttttaataaa
      901 gccgtttatg gaaactacct cccatgaaat aagaaggcag ctaggtattg aagatgagat
      961 aaaatgggag gatacgaaac aatggggact actaaagtca ggaagtaagg ttaataaggg
     1021 agatataatc ttcccaagac tagatattga aaaagaatta gtaagattag acgaggcaaa
     1081 cgaaaaatta atagagcttc gtacaaaaaa tagggtaaag gaagataaga aagtggaaaa
     1141 agatcaatta ataactatag atgattttag caaggtaaaa atgaaactgg cagaagtaat
     1201 aaaatgtgaa gaccatcctc aggctgacaa attattggtt ttaacattga aattaggtga
     1261 ggaaactagg caggtagtat caggaataaa ggaattttac caagcagaag acctggtagg
     1321 taaaaaggta gtcctagtta caaacctaaa acctgtaaag cttaggggta tagaatcaca
     1381 aggaatgatt ttagcagcag aggatagtaa gggagattta tccctattaa ctaccttaga
     1441 ggacttgcag gatggtgcca ccgtatcata ggaggtatat atgctaatag atagtcatgt
     1501 acacttagat gacaaaaggt ttgacaaaga taggcatgag cttatccaat ctttaaaaga
     1561 caatggagta gaattggtaa ttaatatagg ggctgattta agaagcagta ttaattcagt
     1621 aagattggca gaaaaatatg aaaatattta tgctactgta ggtgtccatc cccattcggc
     1681 caaggatgtg gacacagaaa ctattaaaaa attaaaagag ttaacaaaag aaaaaaaggt
     1741 agtagccata ggagagatag gcttagactt ttactatgac aactcgccta gggaactgca
     1801 aagaaaatgg tttaaggaac aactaaagct ggcaaaagag gtagacttac c
//
LOCUS       DATGSI010000274         1286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_115208, whole genome shotgun sequence.
ACCESSION   DATGSI010000274 DATGSI010000000
VERSION     DATGSI010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115208"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VK031_04155"
     CDS             <1..233
                     /locus_tag="VK031_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HLS53141.1"
                     /translation="EYINRVISFLQYLDPSIVVQRIIGRAPEEDSLFVNWDQSWWKIR
                     DEIVEKMERHGFSQGDKFNYLNGKALEKFKSQ"
     gene            285..737
                     /locus_tag="VK031_04160"
     CDS             285..737
                     /locus_tag="VK031_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53142.1"
                     /translation="MVKFILGEKGSGKTKWLIDNANKDMREGNGNIAFVDVDDDHIFS
                     LDYNVRLINAMEFNILNIESFYGFLCGLLSMDYDMEKVYVDSIYKVIDLEISDLENLL
                     ERLTIIGEKMGTEFFINVDYVLDDIPEEMKSYCLELEGIENNVYRVKA"
     gene            complement(785..949)
                     /locus_tag="VK031_04165"
     CDS             complement(785..949)
                     /locus_tag="VK031_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HLS53143.1"
                     /translation="MHTQPEIIEGVKCVVSTCHYHHPGNLCSAGTIEIKPRDATTVEE
                     TDCGTFTPLK"
     gene            1061..>1286
                     /locus_tag="VK031_04170"
     CDS             1061..>1286
                     /locus_tag="VK031_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HLS53144.1"
                     /translation="MSKKQRLDKVLANMGYGSRREVRSIIKSGLVSVNNKVILENGYK
                     VDPYQDLIYCKGEKILYRKHIYLMMNKPKGL"
BASE COUNT          456 a          141 c          273 g          416 t
ORIGIN      
        1 atgaatacat aaacagggta ataagctttc ttcagtacct agatccttca atagtagttc
       61 aaagaataat aggcagggca cctgaagaag actctttgtt tgttaattgg gaccagtctt
      121 ggtggaaaat aagggatgag attgttgaaa aaatggaaag acatggattc tctcaaggcg
      181 ataagtttaa ttatctaaat ggtaaggcat tggaaaaatt taaaagccaa taaattagta
      241 tggtaagtta atattttaaa atatagaaag gctaggtggt taaaatggtt aaatttattc
      301 tgggggagaa gggttcaggt aaaactaaat ggttaattga caatgccaac aaggatatga
      361 gagagggcaa tggaaatatt gcctttgtgg atgtggacga tgaccatatc ttttcccttg
      421 attataatgt acgactaatt aatgccatgg agttcaatat tttaaatatt gaatctttct
      481 atggcttttt atgtgggctc ctttctatgg attatgatat ggaaaaggta tatgttgata
      541 gtatatataa ggttatagac ttggaaatta gtgatctgga gaatttattg gaaagattga
      601 ctataatagg agagaaaatg gggacagagt tttttataaa tgtagactat gtcttagatg
      661 atatacctga agaaatgaaa agctattgct tagaattgga aggtatagaa aataatgtgt
      721 atagggttaa agcataaaaa gatttgtaat taaggcaggg aaatcctgcc ttaattatga
      781 catattattt cagtggtgta aaagttccac aatctgtttc ttctacagtt gtagcatccc
      841 taggtttgat ttcaatggtt cctgctgaac atagattgcc tggatggtga taatgacaag
      901 tacttactac acattttact ccttcaatta tttctggttg ggtgtgcata gtaatctcct
      961 ttcctgaaaa tttatttcag aaatagtttt cctactttaa aaatttatat acaaagcttt
     1021 ttttggcaaa ggcaaattta agatagggtg ataagatata atgagtaaaa aacaaaggtt
     1081 agataaggtt ttggctaaca tgggttatgg tagcagaagg gaagtaagat caataattaa
     1141 aagtggatta gtaagcgtaa ataataaagt tatcttagaa aatggttaca aggtagatcc
     1201 atatcaggat cttatttatt gcaagggtga aaagatatta tacagaaaac acatctacct
     1261 tatgatgaat aaacctaaag gcttgg
//
LOCUS       DATGSI010000275         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_115213, whole genome shotgun sequence.
ACCESSION   DATGSI010000275 DATGSI010000000
VERSION     DATGSI010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115213"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1231
                     /locus_tag="VK031_04175"
     CDS             134..1231
                     /locus_tag="VK031_04175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLS53145.1"
                     /translation="MEKIKVMHVVSDMNFGGAGRYLLEICKYINRERFHLIVVIPIGS
                     ILKEYIGVYDHVEIIEIYGVDTSSFNIKGVKELLSITREVGADLVHSHGCLSARLAAR
                     LLGVKTVYTRHSLVNMEERSKKTLSKLINKFLSSKAIAVSQAIYNNLIENGESVENIY
                     LIYNGVQMPDKEYNQEKLRQKYEIKKDYVITLIGSLEKVKGQEHMLQIVKLLKEKKPN
                     IQILFAGEGSNRSPLERKIITNNLPVELLGHVKDIDEVISLSDVVVNTSNSEAISFAL
                     LEALSHKKPVVAFRIDGIKEVVEDGVNGYLVEFMDYEDFAQKLILLLEDKKLRQKLGQ
                     AGFEKVNKKFQTTDMVKAIEKLYGEIVNEMD"
     gene            1218..1694
                     /locus_tag="VK031_04180"
     CDS             1218..1694
                     /locus_tag="VK031_04180"
                     /inference="COORDINATES: protein motif:HMM:NF025582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HLS53146.1"
                     /translation="MKWIKKLNLLDILIISFFLILLLISLKHLLSFSRRKGTTEPIIY
                     TIQLDKVEESAKEQLQAGKEIFDGNKNYKMGKIKEIQVLSYREEHKNFEEGTIELIEI
                     PDYFKVFLTVKAEANKIGNVFSIKGEEIKVGKDLLIKGKSFIFHGLITSIERLDYE"
     gene            1687..2157
                     /locus_tag="VK031_04185"
     CDS             1687..2157
                     /locus_tag="VK031_04185"
                     /inference="COORDINATES: protein motif:HMM:NF025582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 family protein"
                     /protein_id="HLS53147.1"
                     /translation="MNKKFNWVDYLIIFISILILLLFIYRFYYRDKNQLVNNKDYFKK
                     VVLLKLEEAPEFYKEVFEEGDKIYLTSTDEFVGQIKRLEISRAHTNLIRNDGSLVQAE
                     IPEKYNIYLYVESNLLEREAGYYIAGVTEVLVNNSENFYTDRAIFLATIKAILE"
BASE COUNT          886 a          273 c          499 g          667 t
ORIGIN      
        1 atattaggtt ctttaaccca ggccctggta gatcatattt ggcataatta taaaattatg
       61 cttatgtttt tcttgcaaat ggggctaggg gtcagttctg ttatcttgat gaaaaaggag
      121 caagaaaata aagatggaaa aaattaaagt aatgcatgta gtctctgata tgaattttgg
      181 aggagcggga aggtacttat tagaaatatg taaatatata aacagagaaa gatttcactt
      241 aatagttgta attcccatag gcagtatatt aaaagaatat attggggtct atgatcatgt
      301 ggaaattata gaaatatatg gggttgatac cagttccttt aatattaaag gagtaaaaga
      361 gctattatcc attacaagag aggttggggc agatttggtt cacagtcatg gctgcttgtc
      421 agccagattg gctgccaggc ttttaggggt aaagacagtt tacaccaggc atagcctggt
      481 gaatatggaa gaaagatcta aaaagacctt atctaagctt ataaataagt ttctatccag
      541 taaagcaata gctgtatctc aggcgattta taacaatcta atagaaaatg gggaaagtgt
      601 agaaaatatt tacttgatct ataacggggt acagatgcca gataaggagt ataaccagga
      661 aaaattaaga caaaaatatg aaataaagaa ggattatgtg attaccttaa taggtagtct
      721 agaaaaggta aagggtcagg agcatatgtt gcagattgta aagctcctaa aggaaaaaaa
      781 gcctaatata cagattctgt ttgcgggaga gggttcaaat aggagcccgt tggagagaaa
      841 aataataacc aataatctac cagtagaatt attaggccat gtaaaagata tagacgaggt
      901 tatatcctta tcagatgtgg tagtaaatac ctcaaattct gaggccattt cttttgccct
      961 tttggaggcc ctatctcaca agaagcctgt agtggccttt aggatagatg ggataaaaga
     1021 agttgtagag gatggagtta atgggtattt agtagaattt atggattatg aggattttgc
     1081 tcaaaagctg atcttacttt tagaagataa gaaacttaga caaaaattag ggcaggctgg
     1141 ttttgaaaag gtaaataaaa agtttcaaac aactgatatg gtaaaggcta ttgaaaagct
     1201 atatggggag atagtaaatg aaatggatta aaaagcttaa cctacttgat attttaatta
     1261 tatctttttt cttgatattg cttttgatta gccttaagca tctattgagc ttttcccgta
     1321 ggaaaggtac tacagagcct attatttata caattcagct ggataaggta gaagaatcag
     1381 caaaggaaca actacaggca ggaaaagaaa tctttgatgg aaacaaaaat tataaaatgg
     1441 ggaagataaa agaaattcaa gtcctatctt atagggaaga acacaaaaat tttgaagagg
     1501 gaaccataga gcttatagaa ataccagatt actttaaagt ctttttaact gtaaaagcag
     1561 aagcaaataa aattggaaat gtatttagta taaaaggaga agaaataaag gtaggaaagg
     1621 atctattaat aaagggaaaa agctttatat tccacggtct tattacatct atagaaaggc
     1681 tagactatga ataaaaaatt taactgggta gattatctaa tcatatttat atcaatacta
     1741 atactattat tgtttatata tagattttat tacagggaca agaatcaatt ggtaaataat
     1801 aaggactact ttaaaaaggt tgtacttcta aaactagagg aagctcctga attttataaa
     1861 gaggtctttg aagaaggaga taagatttac ttaactagta cagatgaatt tgttggtcag
     1921 ataaaaaggc ttgaaataag cagggctcat acaaatttaa taagaaatga tggaagccta
     1981 gtccaggcag aaatacctga aaaatacaat atttatttat atgtggaaag taatcttcta
     2041 gaaagggagg ccggctatta tatagcagga gtaacggagg ttttagtaaa taattctgaa
     2101 aacttttata cagatagggc tatattttta gccactataa aagcaatact ggagtagaga
     2161 aaatggaaga aaaaattata ctacaagtct taatgggact agatataggt ggggcggaga
     2221 cacatgtggt ggagctggcc atagaactga aaaaaagagg ttatagggtc atagtagcct
     2281 ccagcggtgg cctatatgag tcaaagttaa aagagaataa tatag
//
LOCUS       DATGSI010000276         1104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_587428, whole genome shotgun sequence.
ACCESSION   DATGSI010000276 DATGSI010000000
VERSION     DATGSI010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587428"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VK031_04190"
     CDS             <1..981
                     /locus_tag="VK031_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53148.1"
                     /translation="LTVDECFMSTENNIYNVDLVKKQQQAIDDSGFYGIPTILKYDGT
                     KVVHGPTDKVPISSYPTGHNENLDAPPVIWEHPISDTPPFGLYCVGVDPYRQGKAEYS
                     DSLGAVYVFKRMHDIYSEKFQDCFVASYVARPDSIIKWQETARWLIRYYNARTLVEND
                     ELSFINYMVYKGDEGYLESKPEWLKSLSSSSTNTNRDFGISTTNPKIRDHVFTKLKTY
                     SEESIDKEVAEDGTIISETFGMSKIPDRVLLEEMKKYDGKINVDRIIAASLAVTLASH
                     LDPIVGKIGGTEEDPRLKGYFNRNKGIKSKNTPSPIITNRYKRNSINIFK"
BASE COUNT          423 a          151 c          210 g          320 t
ORIGIN      
        1 ctcacggttg atgaatgctt tatgtctaca gagaataata tttataatgt agatcttgta
       61 aagaagcaac aacaagctat agatgatagt ggtttttatg gaatacctac aatattaaag
      121 tatgatggaa caaaagttgt acatggtcct acagataaag ttccaatatc ctcatatcct
      181 acaggacata acgagaattt agacgcccca cctgtgattt gggagcatcc tatatcagat
      241 acacctcctt ttggtctata ttgtgttgga gtcgatcctt atcgacaagg gaaggcagaa
      301 tattccgatt ctctgggagc agtatatgta tttaaaagaa tgcatgatat ttattcagag
      361 aagtttcaag attgttttgt ggcctcttat gttgctagac cagacagcat aatcaaatgg
      421 caagaaactg cgagatggtt aataagatat tataatgcaa gaacactagt ggagaatgat
      481 gaactctcat ttattaacta tatggtatac aaaggggatg aaggttactt agagagcaaa
      541 ccagaatggc ttaaatcctt atcaagttct tcaacaaata caaacagaga ttttgggatt
      601 tcaactacta atcctaaaat aagagaccat gtatttacta aacttaaaac atatagtgag
      661 gagagtattg ataaagaagt agcagaggat ggtactatta ttagtgaaac atttggcatg
      721 tcaaagatac ctgatagagt tcttttagaa gagatgaaaa aatatgacgg gaagattaat
      781 gtggatagaa taatagcggc atctttggcg gtaactttag catctcatct tgatccaata
      841 gtaggaaaaa taggaggcac agaggaagat ccaagattaa agggttactt taataggaat
      901 aaaggtataa aatctaaaaa tactccttca cctataataa caaatagata taaaagaaat
      961 tctataaata tatttaaatg attataaaaa atttaaaaca atacgaaaat attattgatt
     1021 ttcctgatca ctttaaatcc gagaaaacaa aaacagagga taaaggatat gtaaaaagga
     1081 caatggattt ttatgcctct aggg
//
LOCUS       DATGSI010000277         3715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_115794, whole genome shotgun sequence.
ACCESSION   DATGSI010000277 DATGSI010000000
VERSION     DATGSI010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115794"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..991
                     /locus_tag="VK031_04195"
     CDS             236..991
                     /locus_tag="VK031_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HLS53149.1"
                     /translation="MTILVFIYGLIIGSFLNVCIYRIPRDESICFPASHCPKCGLKLN
                     WYDNIPMLSYMILKGRCRKCDNFISLQYPIVELVNGLTYVWLYLYFGFTIDFVFYSLI
                     SSVLIIIAFIDLLHMVIPDSLIFVILILNTLQKIILFLFEKIDMNLLDSLLGLLLSGG
                     LFLIIYILSQGGMGDGDVTLIASLGFVLGLKKVILNILLSFFIGALISILLLVTKVKS
                     RKDPIPFGPFMVISFFITLFYGSNIIIWYLNFF"
     gene            1014..2072
                     /gene="pilM"
                     /locus_tag="VK031_04200"
     CDS             1014..2072
                     /gene="pilM"
                     /locus_tag="VK031_04200"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HLS53150.1"
                     /translation="MNIFKGRQILSIDFGSSIIKLVEGKFSPKGVLIDKSFSIDLPEG
                     IYEDGEILDIHRMGSILEENLKENKISASRVNAIINSSQIITRQITMPKVSFDEIQSI
                     LSYQIEDYIPIRPEDYIVQCIALEDVYIDGVQQYKLLLVGIPKDMVESHLNLLRNIQL
                     KPNVLDFQGNATAKLLGQNKLLNDRYKLDEKTIASLDLGYKGTSITIIRDGKIEVARL
                     LDLGVHSLILDIENKTNMGRSNIKDILTSISINEEYEIASKKFQVRSILDQYLSTLLD
                     NIDMVFRYYRTRDAKNRVDLLVLQGGIAEISNLEARFESYFGIESARLLTLDQLDFNQ
                     ALYKYSNAIGGLIRMDGV"
     gene            2075..2677
                     /locus_tag="VK031_04205"
     CDS             2075..2677
                     /locus_tag="VK031_04205"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HLS53151.1"
                     /translation="MKDLNFFDPYIEKGFKISKQFFFNIFVLLSILFLIGYSIFNQLK
                     INKLESRVEEKRKIAEDPKIVKKVEEIKEQEDELKRFEEEVENIRQLNEYIDKTDVVN
                     TSFLQTITSKIPEDMFLIRLNMSYQTVEISGISKDKLNVAQFKKGLEALEPIEDIFIS
                     HITREEDHYTFGMNIHLKEVDLGGEDESEVKESQDSEATD"
     gene            2631..>3715
                     /locus_tag="VK031_04210"
     CDS             2631..>3715
                     /locus_tag="VK031_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53152.1"
                     /translation="MKVKSKKAKILRPLTKKEKILLTILGALIIYFIFYRFVFQRQSD
                     KIESLEEASYQLDQKIAENNRILRNELQIKEDWKNLREERDSILSNYFPVIDQSQIIY
                     LLEELLVDEAIDVSDFRFSRPDLISVADREIAKMDISLPFEGEYNDIIKALQAIQMSP
                     KKILFSSLQFTRDTNNILTGEMILNVISLEGLVAADGQVIYVETIDGNMDNPFLPYGD
                     YREPVRQEDVEDKGEEDENKPEEDIEEALPSELDNKKDESKLEMGSLLYSFQNKEYEF
                     LTNETSIEGLVSPSKMSRLGDTALRLDYQVDKAKDDSFGYIDLDKEHITVKSPVDFLN
                     MGIYSYGANPSQIGLTLMDKNDKSMTL"
BASE COUNT         1309 a          505 c          733 g         1168 t
ORIGIN      
        1 aactacacac tagcaaaaca taaaggtatt tggagagcag cagtagaact tgaagatggc
       61 catcccttcg atgaagaata tgaaggcact tatatacttg gcgaaggaga ccacggatta
      121 gataaggttg atgaataata aacacaatca tcattatttt aaagacaggg aaggtaaact
      181 tccctgtcca tataaattat ttaggttaat ttaagtactt gatacaggtg aaaatatgac
      241 aatattagtc tttatatatg ggcttattat aggttcattt ttaaatgtgt gtatatatag
      301 aatacctagg gatgaaagta tttgttttcc tgcttcccac tgccctaagt gtggacttaa
      361 attaaattgg tatgacaata tccctatgct atcttatatg atcctaaagg gcaggtgcag
      421 gaagtgtgat aattttattt ctcttcaata ccctattgta gaattggtaa atggacttac
      481 ttatgtttgg ttatacttat attttggttt tacaatagat tttgttttct atagcctgat
      541 ttcatcagtt ttgattataa ttgcttttat tgatctattg catatggtaa taccggatag
      601 cttaatattt gttattttaa tattaaatac tttacaaaaa atcatccttt ttctctttga
      661 aaaaatagat atgaatcttt tagatagcct attggggctc ttgctttcag gaggcttatt
      721 tttaataata tatattctat cacagggtgg tatgggagat ggagatgtga cccttattgc
      781 atccttaggt tttgttctag gccttaaaaa ggttatttta aacattctac tgtccttttt
      841 tataggggct ttaatatcca tacttttact ggtaaccaaa gttaagtcaa ggaaggatcc
      901 aatccccttt ggacccttta tggtcatatc ttttttcata acactatttt acggctcaaa
      961 tattataatt tggtatctaa acttttttta aatttctttg aaggcgggat aatatgaata
     1021 tttttaaagg cagacagatc ttgtccattg attttggatc ttccataata aagctggtgg
     1081 aagggaagtt ttctccaaaa ggagtcctaa tagataaaag tttttctata gacttacctg
     1141 aagggatata tgaagatggg gaaatattag atatccatag aatgggaagt attttagagg
     1201 aaaatctaaa ggaaaacaag atttctgcca gtagagtcaa tgcaattata aattcttccc
     1261 aaataattac caggcaaatt actatgccaa aagtatcttt tgatgaaatc caatcaattt
     1321 taagctatca gattgaggat tatattccta ttaggccgga ggactatata gttcaatgta
     1381 ttgccctgga ggatgtatat atagatggag tccagcaata caagctattg ttagttggaa
     1441 tccctaaaga tatggtagag tcccatttaa atcttttaag aaatatccag ctgaaaccca
     1501 atgttttaga ctttcaagga aatgcaacgg caaagctact tgggcaaaac aagctgctta
     1561 atgatcgtta caagcttgat gaaaaaacaa ttgcttcttt agatttaggt tataaaggta
     1621 ccagcatcac aattataagg gatggaaaga ttgaagtggc caggttattg gatcttggag
     1681 ttcatagctt aatcttagat atagaaaata agactaatat gggaaggtca aatattaagg
     1741 atatattgac cagtatatct ataaatgaag aatacgaaat agctagtaaa aagtttcagg
     1801 taagaagtat tctcgaccaa tatttgtcta ctcttctaga caatattgat atggtattta
     1861 ggtactacag aactagggat gcaaaaaata gggttgacct acttgtacta cagggaggta
     1921 ttgctgagat ttccaattta gaagccaggt ttgaaagcta ttttggcata gaaagtgcaa
     1981 ggcttttaac cttagatcag ctggatttta atcaagcctt gtacaagtat tctaatgcta
     2041 ttggtggcct aataaggatg gatggggtgt agttatgaaa gatttaaact tttttgatcc
     2101 atatattgaa aaaggattta aaataagcaa acaatttttc ttcaacatct ttgtgctcct
     2161 aagtatactg ttcttaatag gctacagtat atttaatcaa ttgaagataa acaaattaga
     2221 gtctagggtg gaagaaaaaa gaaaaattgc agaggatcct aaaatcgtaa aaaaagtaga
     2281 ggaaataaaa gaacaagaag atgaattaaa aaggtttgaa gaagaggtag aaaacattag
     2341 acaactaaat gaatatatag acaaaactga tgtggtaaat actagtttcc ttcagactat
     2401 taccagcaaa ataccagagg atatgttttt aattcggcta aatatgtctt atcaaacagt
     2461 ggaaatttca ggtatatcca aggataaatt gaatgtggcc caatttaaaa aaggtttaga
     2521 agctcttgag cctatagagg atatttttat ctcccacata accagggaag aagatcacta
     2581 cacatttggc atgaatattc atttaaagga ggtggacctg ggtggagagg atgaaagtga
     2641 agtcaaagaa agccaagatt ctgaggccac tgactaagaa agaaaaaata ttgttaacta
     2701 tcctaggggc cttgattatt tactttattt tttatagatt tgtttttcaa aggcaatcag
     2761 ataaaattga aagtctggaa gaggcaagtt accagctgga tcaaaaaatt gcagaaaata
     2821 atcgtatcct aagaaatgaa ttgcaaatca aggaagattg gaaaaatctt agggaagaaa
     2881 gggatagtat cttgtccaac tattttccgg taattgatca atctcaaatt atctatcttt
     2941 tggaggagtt actagtagat gaggcaatag atgtatcaga ttttagattc tctaggccag
     3001 atctaattag tgtagccgat agagagatag ctaaaatgga tattagtcta ccctttgaag
     3061 gggaatataa tgatataatc aaggctttac aagccataca gatgagtcct aaaaagattt
     3121 tatttagttc cttacaattc accagggata caaacaatat acttacaggt gagatgattt
     3181 taaatgtaat tagcctggag ggacttgtgg ctgctgacgg gcaagtaata tatgtggaaa
     3241 ctattgatgg gaatatggac aatcccttct tgccatatgg tgactataga gaacctgtaa
     3301 gacaggagga tgtggaagac aaaggagagg aagatgaaaa taagcctgag gaagatattg
     3361 aggaagctct tccttcagaa ttagataata aaaaggatga aagtaaactg gaaatgggaa
     3421 gtctcctata ctcctttcaa aacaaggaat atgaattttt aaccaatgaa acctccatag
     3481 aaggactagt atctccttct aaaatgagta ggctaggaga tactgcctta agattggact
     3541 accaggtaga taaggccaaa gatgatagct ttggatatat tgatttagat aaggagcata
     3601 taacagttaa aagtccagtt gactttttaa atatggggat atattcttat ggggcaaacc
     3661 ctagccagat aggcttaact ttaatggata aaaatgataa aagcatgact ttaag
//
LOCUS       DATGSI010000278         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_540370, whole genome shotgun sequence.
ACCESSION   DATGSI010000278 DATGSI010000000
VERSION     DATGSI010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540370"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1063)
                     /locus_tag="VK031_04215"
     CDS             complement(59..1063)
                     /locus_tag="VK031_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004829864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS53153.1"
                     /translation="MALLEVKNLHTYFETKRGTVKAVNGVSYSVEAGKTLGVVGESGS
                     GKSVSAMSIIKLLDGNGYIDQGEIIFDGKDLVKVPINEMQHIRGNDISVIFQEPMTSL
                     NPVFTVERQISEPFIIHQGMSKKEASKQVVKMLSLVRIPNPEAVAKQYPHQLSGGMRQ
                     RVMIAMALACKPKLLIADEPTTALDVTIQAQILKLMNELKEETNTSILFITHDLGVIN
                     EMADDVAVMYCGQVVEKAPAKDIFGDKSKYSHPYTEGLMYSIPRLDTPADTRLEAIPG
                     AVPHPLNLPKGCKFAPRCKYATDKCKQVEPELLEIEEGHEIRCHYPEKGVRSNGKKRE
                     "
     gene            complement(1076..>2544)
                     /locus_tag="VK031_04220"
     CDS             complement(1076..>2544)
                     /locus_tag="VK031_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLS53154.1"
                     /translation="KTRITATTNLRNFPTDPDTNQKLDMGKVVKVAAGFDHALALNEE
                     GEVFGWGSDRHKQARVPNEIKRLNNIKDIYAGYQCSLVLTEDGHIHYFGNKNLMDYNE
                     FHEYQGQIDKLTLTQDAVLGLTFDGEVVYLGTEQNSYARIPENMGKITDISSTSANIA
                     AVNEDGEIFVWGNISSQSRENEVPEFDSKIVAIEGGRRHYTALTESGKLYSWGSDFYK
                     ESVIPKKIQDKEFENIYVNYYQNYAVDSEGKVATWGLKGYLMGTDNLGRDIFTRLLNG
                     GRMSMTIGGVAVIISTIIGVIVGSLSGYFGGTIDIILQRLAEIVESLPFLPFAMILSS
                     LIGNSMNTNQKIVLIMVILGLLSWTGLQRLIRAQILSVREQEYVVAARALGIKESGII
                     FRHILPNVISVIIVSATLSFATSMLTEASLSYLGFGVQPPQPTWGNMLYGANNSQVIQ
                     NYWWRWVFASALLGICVICINLIGDGLRDAIDPKSQER"
BASE COUNT          687 a          572 c          404 g          881 t
ORIGIN      
        1 gtccttctta actgggaaat atttcttcag gttctccata cgaagcagaa cttttctatt
       61 attctctttt tttgccattt gatctcaccc ctttttctgg ataatgacat cttatttcat
      121 gcccttcttc gatttctagt aactctggct ctacctgctt acatttatca gttgcatact
      181 tacatctagg ggcaaatttg caacccttag gtaagtttaa gggatgaggc acagctcctg
      241 gtatggcctc cagccttgtg tctgcaggcg tatccagtct tggtattgaa tacattaggc
      301 cttctgtgta ggggtgggag tacttactct tatcaccaaa tatatcctta gctggtgcct
      361 tctccaccac ctgtccacaa tacattactg ccacatcatc tgccatctca tttataaccc
      421 ctagatcatg ggtaataaac agtatggagg tattggtctc ctcctttagt tcattcatca
      481 attttaaaat ctgagcctga atagtaacat ctaaggctgt ggttggctca tcggctatga
      541 gtaatttggg cttacaggcc aaggccatgg caatcataac cctttgcctc attccaccag
      601 ataattggtg gggatattgt ttggccactg cctcaggatt tggaatcctg actaaggata
      661 gcatcttaac tacctgtttg ctggcctctt ttttactcat accctgatga attataaagg
      721 gttctgaaat ctgtctttcc actgtaaaga ctggattcaa tgaggtcatt ggttcttgga
      781 atatgacgga tatatcatta cctcttatat gttgcatttc atttataggg acctttacca
      841 aatcttttcc atcaaaaatg atttctccct gatctatata accattgccg tccaagagtt
      901 ttataatact catggctgac acgctttttc cacttccact ttcacctact acacctaggg
      961 tcttaccagc ttcaacagaa taggatacac catttactgc ctttactgtt cctctcttgg
     1021 tttcaaaata ggtgtgcaag tttttcactt ctaatagtgc catctcatct tcctcctaac
     1081 gttcttgaga ttttggatct atagcatctc ttagtccatc ccctattaag tttatgcaaa
     1141 ttacacaaat acccaatagg gctgaagcaa agacccatct ccaccagtag ttttgtataa
     1201 cctgactatt attggctcca tataacatgt tgccccaggt tggttgcgga ggttgtacac
     1261 caaatcctag ataggacagg ctagcctctg taagcatact ggtagcaaag gataaagtgg
     1321 ccgatacaat aattacagaa attacatttg gcagtatatg cctaaatatt attccacttt
     1381 ctttgattcc aagagctcta gcagcaacca catactcttg ttctctaact gacaaaattt
     1441 gagcccttat tagtctctgc aatccagtcc aagatagtaa ccctaagatt accataatca
     1501 atactatttt ttgatttgta ttcatactat ttccgattaa ggaagataat atcatagcaa
     1561 atggtaggaa tggtaaagat tctacaatct ctgccagtct ttgtaaaata atgtctatgg
     1621 ttcctccaaa gtaacctgag aggctaccta ctataactcc tattattgtt gagattataa
     1681 cagcaactcc acctatggtc atagacattc gtccaccatt taacaatcta gtaaatatat
     1741 ctctacctag gttatcggtt cccattagat accctttaag cccccaagta gctacctttc
     1801 cttcactgtc tactgcataa ttttgatagt aatttacata tatattttca aactctttat
     1861 cttgaatttt cttaggtata actgattctt tatagaagtc agaaccccaa gaatataatt
     1921 ttccactttc tgttaaggca gtataatgtc ttctaccacc ttctatagca acaatcttgc
     1981 tatcaaattc tggtacttca ttctccctgc tttgggaaga tatattaccc caaacaaata
     2041 tttccccatc ttcatttact gctgctatat tggcagaggt ggaagaaata tcggtgattt
     2101 tacccatatt ttcaggtatc cttgcatagg agttttgttc tgtaccaaga tatactactt
     2161 ctccatcaaa ggttaaccct aatacagcat cttgagttaa ggttaacttg tctatttgcc
     2221 cctgatactc atgaaactca ttataatcca ttaagttttt gttaccaaaa tagtgtatat
     2281 ggccatcttc tgtcagtacc aatgaacatt gataccctgc atatatatcc ttaatgttgt
     2341 ttaatctctt tatttcattg ggtactctgg cttgtttatg tctatcacta ccccagccaa
     2401 agacttcccc ttcttcattt aaggctagag catggtcaaa gcctgctgca acctttacta
     2461 ccttacccat atccagcttt tgattagtat ctggatctgt tgggaaattc ctaagattgg
     2521 tggtagcagt aattcttgtt ttac
//
LOCUS       DATGSI010000279         5016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_443314, whole genome shotgun sequence.
ACCESSION   DATGSI010000279 DATGSI010000000
VERSION     DATGSI010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443314"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VK031_04225"
     CDS             complement(<1..743)
                     /locus_tag="VK031_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HLS53155.1"
                     /translation="MKVVGITTQQEVFVGSKERNFRINEFLIIEDPFQGELLGEVVEA
                     QTFNRYIPLNIYGDFVDNSVLESLKALGYDIDEDTIYISKIRLLNEALYPIQTGSDVR
                     LPEFIEVKDIMIKGDKEDGLVLGIIKNTDDMYEEMDDELKDLLYIFEEGRLKPQNEIP
                     YIMDLKSMHHYPHIGVFGGSGSGKSFGLRVLLEELMEQQVPTIVLDPHYEMDFGEGAH
                     YLEETIDFKDKFKCLQVGTHVGVRFEDLSA"
     gene            complement(762..1748)
                     /locus_tag="VK031_04230"
     CDS             complement(762..1748)
                     /locus_tag="VK031_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HLS53156.1"
                     /translation="MYSLSDKLKERVTDLNQVLKTKYDQVLNLNKSNLRRFIKENIGT
                     IKSMEKLDNKHLALYEAKGGIVGVDGSNNRIGGAYPHFVEAYQGLAKSTLHKNEPLIL
                     ADIYSPLNPEQEENPLEDDENIVEETRNIKLATIEVEVALASIERFRPYAIMMDGGLI
                     RYNIYAYDRWMELRNKCEDEGILLIGVIKDIKTSIIGDKMRELNPNMEEYFYDKELLF
                     GQLSYGELILIDDQVNKKSPEGYASAFLRSSLAPNVIGMDILDSQKQHMEEMARLVFT
                     LTPENSRGVPLWLDMIDKEVKISDEIMKALMERYMDRGIYERFFVSERDRRN"
     gene            complement(1773..2597)
                     /gene="rsmI"
                     /locus_tag="VK031_04235"
     CDS             complement(1773..2597)
                     /gene="rsmI"
                     /locus_tag="VK031_04235"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HLS53157.1"
                     /translation="MKTANLFVCPTPIGNLEDISLRTLKVLEKVDLIAAEDTRHTRKL
                     LNHYNIKNSLTSYHEHNEREKGEKLIEKLKEGKNIALVSDAGMPGISDPGEKLIRLAI
                     EADIKVVVLPGPTAAITALVASGLATDKFAFEGFLSSRKGDRRKELEKIKNEERTLIF
                     YESPYRLLDSLKDMLNILGDRQVAIARELTKLYEEFFRGQLSQAIKKFQADQIRGEFV
                     IVVEGTSKEEKDIVIDIEKKLKKYLDKGYTKKDSIRLVAEKYGLPRNQVYKESLKI"
     gene            complement(2598..3332)
                     /locus_tag="VK031_04240"
     CDS             complement(2598..3332)
                     /locus_tag="VK031_04240"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HLS53158.1"
                     /translation="MENKNLRIDWVPGTRYKIIQNREAFSYGMDAILISNFSKATGKV
                     MDLGTGSGIIPIRLADEKRVKKIYGVEIQKYVAQLAQASVELNGLEEKIEILHMDLRK
                     LTERFGKSTFDTIISNPPYMRAGSAIVNREENFALARHEITCNFEDIIRIADYLLKPL
                     GKVYIVHRPDRLTDIFYTMRKYKIEPKYIRFVQAKLNRRPNLILLEGIKHGKPDLKFF
                     HPLIVYKDDGTYTDEIYEIYGMDRVR"
     gene            complement(3438..4310)
                     /locus_tag="VK031_04245"
     CDS             complement(3438..4310)
                     /locus_tag="VK031_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HLS53159.1"
                     /translation="MIDVVGVRFKKAGKIYYFDPDNIDVGFHDFVIVETARGIEFGHV
                     VIGPKKVAEEEIVSPLKKVLRVATAKDFEIHRINIEKAKKAMTICQEKVEEHGLGMKI
                     IDVEYTFDNNKIIFYFTADGRVDFRELVKDLAAIFRTRIELRQIGVRDEAKMIGGLGP
                     CGKPCCCAQFLGEFEPVSIKMAKEQSLSLNPSKISGLCGRLMCCLKFEQEAYEEILEK
                     MPEVGTMAITPVGRGTIIETYTLLETVKVRVRLEDGVEDVVQYHIGDIVITDEIHPDY
                     KKEEVAYSERKDAQ"
     gene            complement(4321..>5016)
                     /locus_tag="VK031_04250"
     CDS             complement(4321..>5016)
                     /locus_tag="VK031_04250"
                     /inference="COORDINATES: protein motif:HMM:NF020680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HLS53160.1"
                     /translation="IEGLIEEMSTAPFESGRKVFIIDDSHKMNKESENALLKTLEEPP
                     GFVIIILITSKPDSLLTTILSRVQTIKFFPLKVDKIANLLIDKYKKSEDEAYYIAEFS
                     KGALGKSIRLAEDMDFFQRREEVLALIAALLKGDKSKIFSSTNFFKDNREHIDEILDI
                     FTYWFRDLIIYKETRRKDFLINRDKVEDLSKQANMNLDKINDIINSIMETRTNINRNI
                     NYQLAIENMLLNI"
BASE COUNT         1379 a         1140 c          654 g         1843 t
ORIGIN      
        1 gccgataagt cttcaaacct tactccaaca tgggtaccta cctggagaca tttaaactta
       61 tccttaaagt caatggtctc ctccagataa tgggcacctt caccaaaatc catttcataa
      121 tgaggatcta gtacaatggt aggcacttgt tgttccatta actcctctaa aagcacccta
      181 agtccaaaag atttccctga accggaacct ccaaaaacac ctatatgggg ataatggtgc
      241 atagacttta ggtccattat ataaggaatt tcattttgcg gcttaagcct accttcttca
      301 aatatatata acaaatcctt tagctcatcg tccatttctt catacatatc atctgtattt
      361 ttaattatac ctaaaaccaa gccatcttcc ttatctccct ttatcattat atcctttacc
      421 tctataaact ccggtaatct tacatcagag cctgtttgta taggatataa ggcctcgttt
      481 aatagcctta ttttagaaat atatattgta tcttcatcta tgtcgtagcc taaggcttta
      541 agggattcta atacagaatt gtctacaaaa tctccgtata tatttaaggg aatatatctg
      601 ttaaaggttt gagcctccac cacttctcct aataattctc cctggaaggg atcctctatt
      661 attaaaaatt catttatcct aaagttccgt tcctttgaac ctacaaatac ttcctgctgg
      721 gttgtaattc ctactacctt catggtccac caccttcttt actaatttct cctatctctt
      781 tctgatacaa aaaacctttc atatattcct ctatccatat acctttccat caaagccttc
      841 ataatctcat cagatatttt tacctcttta tctatcatat ccagccataa ggggactccc
      901 ctactatttt ctggagtcag ggtaaatact agcctggcca tctcctccat atgttgtttt
      961 tgactatcta atatatccat tcctattaca ttaggagcca gcgaggatct taaaaaagct
     1021 gatgcgtagc cttccggtga ctttttgtta acctgatcat ctattagaat caactctcca
     1081 taggataatt ggccaaataa caattcttta tcataaaaat actcctccat atttggatta
     1141 agctctctca ttttgtcacc tattatggaa gtttttatat ccttaatcac gcctattaga
     1201 agaattccct catcttcaca tttatttctc aactccatcc acctgtcata ggcataaata
     1261 ttatatctta ttaggccccc atccatcatt atggcataag gcctaaatct ttctatgctg
     1321 gccagggcca cctccacctc tatggtggcc agcttaatat ttctagtttc ctctactata
     1381 ttttcatcat cctccaaagg attttcttcc tgttctgggt ttagaggact atatatatct
     1441 gctaaaatta ggggttcatt tttatgaagg gttgacttgg ccaagccctg gtaggcctct
     1501 acaaaatgag gataagcacc tcctatccta ttatttgacc catctacccc tactatgcca
     1561 cccttagcct catataaggc caaatgttta ttatctagtt tttccataga ctttatggtc
     1621 cctatatttt cttttataaa cctacgtaaa ttagatttat ttaaatttaa gacctgatca
     1681 tacttagttt ttaaaacctg atttaaatct gtaacccttt cctttaattt atcactaagg
     1741 ctatacattc tgccaccccc tcatgttgct agttaaattt ttagactttc tttatatacc
     1801 tgatttctag gcaagccata tttttctgcc accagcctaa tactatcttt ctttgtatat
     1861 cccttgtcca aatatttttt tagtttcttt tctatatcta ttactatatc cttttcctct
     1921 ttactagttc cctccaccac tattacaaac tctcctctta tttggtctgc ctgaaacttt
     1981 tttattgctt gggatagctg acctctaaag aattcttcat aaagcttagt caattccctg
     2041 gctatggcca cctgcctatc tcccaatata tttaacatat ccttcagact atctaagagt
     2101 ctatagggtg actcataaaa aattagagtt ctttcttcat tttttatctt ttctaattcc
     2161 ttccttctat caccttttct agaggaaaga aagccttcaa aagcaaattt atctgtagcc
     2221 agtccagatg ctaccagggc tgtaatagct gctgttggtc ctggtaatac taccaccttt
     2281 atatctgcct ctatggccaa tcttatcagt ttctctcctg gatcagaaat ccctggcata
     2341 cctgcatcag aaactagggc tatatttttt ccttccttta atttctctat aagcttttct
     2401 cccttttccc tttcattatg ttcatgatag ctggtaaggg aatttttaat attgtagtga
     2461 tttaacagtt ttcttgtgtg tcttgtatct tctgcagcta ttaaatctac tttttctaga
     2521 accttaagcg tcctcaagct tatatcctct aaatttccaa tgggcgtggg acatacaaat
     2581 aggttagctg ttttcatcta tctcacccta tccattccat aaatttcata tatttcatct
     2641 gtatatgtgc catcatcttt gtacactatt aagggatgga aaaatttcaa atctggtttt
     2701 ccatgcttta ttccctccaa taagattaaa ttaggccttc tatttaactt agcctggaca
     2761 aacctaatat attttggctc tattttatat tttctcatgg tgtaaaatat atctgtcagc
     2821 ctatctggcc tatgtactat atagaccttg cccaaaggct ttaacaaata gtcagcaatc
     2881 ctgattatat cttcaaaatt acatgtaatt tcatgtcgtg ccagggcaaa gttctcttcc
     2941 ctgttgacta tggcagatcc agccctcata taaggtggat tggatatgat ggtatcaaag
     3001 gtagattttc cgaatctttc agttagtttt ctaagatcca tatgtaagat ttctatcttt
     3061 tcttccaagc catttaactc aacacttgcc tgggccagct gggccacata tttttgaatt
     3121 tctaccccat atattttttt aacccttttt tcatctgcca gcctaatagg tatgatccct
     3181 gaacctgtcc ccagatccat caccttacct gtagccttag aaaaatttga aataaggatg
     3241 gcatccatcc cataggagaa ggcttccctg ttttgaatga ttttgtatct tgttcccggt
     3301 acccagtcta ttcttaaatt tttattttcc aaattaccac cccatttact aactaataat
     3361 atcaaaatcc cggccaaatg tatatatgtt tgctataagc ttaaagattg tataagaaaa
     3421 aagaccctaa gggtctttta ttgtgcatct tttctttcac tataagctac ttcctctttt
     3481 ttataatctg gatgtatttc atcagtgata actatatctc ctatatgata ttgtactaca
     3541 tcctctactc catcctctag tcttactctt accttgacag tttcaagcag agtataggtc
     3601 tctataatag ttcctctccc aactggagta atggccatgg tcccaacctc aggcattttt
     3661 tccaatattt cctcgtaggc ctcctgttca aattttaggc aacacattag cctaccacat
     3721 aggccagata ttttggaagg atttaaggat aggctttgct ccttggccat tttaatagat
     3781 acaggttcaa actctcccaa aaattgggca cagcaacaag gcttaccaca gggccccagc
     3841 cctcctatca tttttgcctc atctcttact cctatctgcc ttagttctat cctggttcta
     3901 aatatggctg ccaaatcttt aaccaattct ctaaaatcta ctctaccatc tgctgtgaaa
     3961 taaaatatta ttttattgtt gtcaaaggta tactccacat ctattatttt catgcctagt
     4021 ccatgttcct caaccttttc ttggcaaatg gtcatggctt tctttgcctt ttctatatta
     4081 atcctatgta tttcaaagtc tttggctgta gccaccctaa gcaccttctt taaaggtgat
     4141 acaatttctt cttctgccac cttttttggt cctattacta catgtccaaa ttcaattccc
     4201 ctggccgtct caactattac aaaatcgtga aatcctacat ctatattatc agggtcaaaa
     4261 tagtaaattt tccctgcctt cttaaaacgt actcccacca catcaatcat gtataccctc
     4321 ctatatatta agtagcatat tttctatggc taactgataa ttaatatttc tattaatatt
     4381 tgttctagtt tccattatac tatttattat atcattaatc ttatctaaat tcatattagc
     4441 ctgtttagat aaatcttcta ctttatccct attgattaag aaatccttcc ttctagtttc
     4501 tttataaatg ataaggtccc taaaccaata ggtaaatatg tctaaaattt catctatatg
     4561 ttctctatta tctttaaaaa aattggtgct actaaatatt ttacttttat ctcccttaag
     4621 cagagcagct ataagggcta gaacttcttc ccttctttgg aaaaagtcca tatcttccgc
     4681 cagtctaata gatttaccta aggctccctt agaaaactca gcaatataat aggcctcatc
     4741 ctcacttttt ttatatttat ctattaaaag attggctatt ttatcgactt taagtgggaa
     4801 aaatttaata gtttgtaccc ttgacaagat agttgtcaat aagctatccg gcttagatgt
     4861 aattaggata ataataacaa agcctggtgg ttcctccaag gtctttaaaa gggcattttc
     4921 actttccttg ttcattttat ggctatcatc aattatgaaa accttcctgc cagactcaaa
     4981 aggggctgtg gacatctctt caattagacc ttctat
//
LOCUS       DATGSI010000280         1683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_251714, whole genome shotgun sequence.
ACCESSION   DATGSI010000280 DATGSI010000000
VERSION     DATGSI010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251714"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..357
                     /locus_tag="VK031_04255"
     CDS             109..357
                     /locus_tag="VK031_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53161.1"
                     /translation="MDVNRKMIELWGDVVDIKHLIYSILISAISTMGAYFFAPANNNT
                     KGLFYGLMGAVLGFIISTFIFKPKRIIKFQTDIINDKE"
     gene            362..>1683
                     /locus_tag="VK031_04260"
     CDS             362..>1683
                     /locus_tag="VK031_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HLS53162.1"
                     /translation="MENLPLLQMTLAALGAVILYIIIGFIPGTDETSVLMPITLTVVL
                     AGVEPIIVLTFFIAAIVTLNLTNAIPVALVGLPGGVMSSPMMEHSLYLKERGLSAMVI
                     KKMAVGSLIGTVVSIPVALILSNILAPYASTIQNFAPLLFFVGAIFLSLIGKNKILAL
                     LSIVPVSVLFMGLRHLYWGIGLVPQDKNITTSFFLGITVGPLIVSLLSLLNRDKLKEV
                     VINEKKNITIPANVLENKTLNPFKILTKEEMGKAILPALISNFLFVLSPVGLTILFGE
                     AMASREEDPIKRATTAITTMSALGQSTYLSGIIIPLIALGIPLSPTAIGPGGALFNAP
                     PIFSVDNNIHHLLNKGEFIAAILIGALVAVAINYYVINRFAGQISTFVLKKIPHEAII
                     GLFISLILLLSYMDAGLANVFGVLLIGITCGTLNKMGVNYGVQFMTLYA"
BASE COUNT          533 a          291 c          321 g          538 t
ORIGIN      
        1 atctagattg attcccttaa ttttaagagg accatataaa agaaaaattg actatatatc
       61 tataatttca gttgaaataa aacactttat tattaaggag gtttgttaat ggatgttaat
      121 agaaagatga ttgagctttg gggagatgtg gtagatataa aacatctaat atactccata
      181 ttaatctcag ccatttctac catgggagcc tacttctttg ctccagcaaa taataacacc
      241 aaagggttat tttatggttt aatgggggca gttttaggct ttataatatc tacctttata
      301 ttcaagccta aaaggattat caaatttcaa acagatataa taaatgataa ggagtaacat
      361 tatggaaaac ttaccattat tgcaaatgac attagcagcc ttaggggctg tgattttata
      421 tataataata ggttttatac caggaacaga cgaaacatcg gttttaatgc ccattacatt
      481 gacagtggtg ctagcaggtg tggagccgat aatagtttta acctttttca tagcagccat
      541 agtaacctta aacttaacca atgccatacc tgtggccttg gtaggcttgc ctggtggtgt
      601 aatgtcaagt cctatgatgg agcattcact ttacttaaag gaaaggggcc tatctgccat
      661 ggtaataaag aagatggcag ttggatcctt aatagggaca gtagtatcta tacctgtggc
      721 cttaatacta tcaaatatac tggcacccta tgccagtact attcaaaact ttgctccctt
      781 attattcttt gtaggggcaa tctttctatc gctaattggt aaaaataaga tattggccct
      841 attaagcata gttccagtgt cagtcctttt tatgggctta aggcacctat actggggaat
      901 tggcttagta ccacaggata agaatattac cacttccttc tttttgggta taactgtagg
      961 acccttaata gtttccttat tatcgctttt aaacagggac aagctgaagg aagttgttat
     1021 aaatgaaaag aaaaacatta ctataccggc caatgtgtta gaaaataaga ccttaaatcc
     1081 ttttaaaata ttaaccaaag aagaaatggg taaggctatc ttgccggctt tgatttcaaa
     1141 ctttttattt gtcttaagtc cagttggatt aaccatactt tttggtgaag ccatggcatc
     1201 tagggaggaa gacccaataa aaagggctac aacagctatt acaaccatga gtgctttggg
     1261 gcaatcaact tatctatcag gaataattat tcctctgata gctctaggga tcccactgtc
     1321 tcctacggcc atagggccag gaggagcctt atttaatgct ccgcctattt tttcagtaga
     1381 taataatatt catcatctac taaataaggg agaattcata gcggccatcc taatcggagc
     1441 cttagtggca gtagccataa actactatgt aataaataga tttgccggcc agatatccac
     1501 ctttgtatta aaaaagatcc cccatgaggc tataattgga ctatttattt ctctgatact
     1561 tttactatcc tatatggatg cagggttggc taatgtattt ggggtcctgt taataggaat
     1621 tacctgtggt acactaaata agatgggagt aaattatggt gtacaattta tgaccctata
     1681 tgc
//
LOCUS       DATGSI010000281         1272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_634822, whole genome shotgun sequence.
ACCESSION   DATGSI010000281 DATGSI010000000
VERSION     DATGSI010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634822"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VK031_04265"
     CDS             <1..368
                     /locus_tag="VK031_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLS53163.1"
                     /translation="LEKIFNVKISSIEDINKYGKKIIEMGAENAIVSYGKKGSMFFSK
                     DGKSYIAKEIDLDKVSNTIACRDAMIGGFIGTLVKTSDTLKAFKLAVAAATATARVVD
                     LPSRSEIEKYIDEVDIKEL"
     gene            420..>1272
                     /locus_tag="VK031_04270"
     CDS             420..>1272
                     /locus_tag="VK031_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53164.1"
                     /translation="MNKKTVSIMLAYVGVLTGAGLASGQEIMQYFISFGKIGLLGLVG
                     IGILHVLIGGVVLQLGSHYLAKSHIDVLEEVSEIKYVTKFMDFALIVNCFLMGFVMIA
                     GAGSNLEQQFGFKPWVGALICTLLIIFIGMLDFDKVTSVIGAFTPLIIIFTIIGAIYT
                     FVNVDPDWELLDKLGKSLINPLPNIGLSTINYFGLCMMSGLSMAFVLGGSNEDSAEAG
                     IGGMMGGALVAVLTGFVGMTLFLSLETAGGSDIPMQIVLRNVHPYLGLIMSLIIFGMI
                     FNTSISLF"
BASE COUNT          418 a          189 c          260 g          405 t
ORIGIN      
        1 acttagagaa aatttttaat gttaaaatct cttcaattga agatataaac aagtatggga
       61 aaaaaattat tgaaatggga gctgaaaatg ccatagtatc ctatggtaaa aaaggatcaa
      121 tgtttttttc aaaagatggt aaatcctata tagccaaaga aatagaccta gacaaggttt
      181 caaacaccat agcctgtagg gatgctatga ttggtggatt tataggaact ttagttaaaa
      241 caagcgatac cctaaaagcc ttcaaacttg cagtagcagc agcaactgca acagcaagag
      301 tggttgacct acctagtaga agtgagatag aaaaatatat agatgaggtt gatataaaag
      361 agctttaata gtttatttct agttttgtaa gtgtttttta tagaaattaa ggagaaatta
      421 tgaataaaaa gacagtaagc atcatgcttg cttatgtagg tgtgcttacc ggagctggcc
      481 ttgcaagcgg ccaggaaatc atgcaatatt tcatatcctt tggaaagata ggtcttctag
      541 gccttgtagg aataggaata ttacatgtac ttattggagg agtagtactc caacttggat
      601 ctcactatct tgccaaaagt catatagacg ttttggaaga agtatcagag ataaaatatg
      661 tgactaaatt tatggacttt gccctaatag taaactgctt tttgatgggc tttgtaatga
      721 tagcaggagc aggatcaaat cttgagcaac aatttggctt taagccatgg gttggagcct
      781 tgatatgtac acttttaatc atatttatag gtatgctaga ttttgacaag gtaacaagcg
      841 taattggtgc ctttacccca cttattatta tctttaccat aataggtgca atctatacct
      901 ttgtaaatgt ggatccagac tgggaacttt tagataaact tggcaaaagt ttaataaacc
      961 ccctaccaaa tatagggctt tctaccataa attattttgg cctatgcatg atgagtggtc
     1021 tttccatggc ctttgttttg ggtgggtcaa atgaagattc agctgaagca ggaattggtg
     1081 gaatgatggg aggagcactt gtagctgtac ttacaggctt tgtaggaatg accttatttt
     1141 taagtcttga aactgcaggt ggatctgata taccaatgca aatagtactt agaaatgtcc
     1201 atccatatct aggtcttatt atgtcactta ttatatttgg aatgatattt aacacatcca
     1261 tatccctatt tt
//
LOCUS       DATGSI010000282         5226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_210418, whole genome shotgun sequence.
ACCESSION   DATGSI010000282 DATGSI010000000
VERSION     DATGSI010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210418"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VK031_04275"
     CDS             complement(<1..1305)
                     /locus_tag="VK031_04275"
                     /inference="COORDINATES: protein motif:HMM:NF015746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HLS53165.1"
                     /translation="MTAKAKDQNKGFLAWVERVGNRIPHSFILFVWLIVIIAVISFIA
                     SKVGLQVENPTSGEIVEARNLISGEGINFALSSAVSNFTGFAPLGLVLTMALGIGLAE
                     NVGLMSSFMRKTILGASPKLVTFMIMLIGINGNIASDAAIVIIPAISAMIFLSLGRNP
                     LAGIALGYAATTAGFSANFFIAGTDGLLSGITNEASQIVGAPEIPVTANWYFMATSTV
                     ILAIVGTLISDRIIEPRLGEYTGNKQIEDQEVSELESKGLKAAGIGLAIYLAALIILA
                     APKSSFLRNPETGSLISGSPLLAGIIPLLLILFLVTSIPYGKVVGKIKSSGDIPKYMT
                     MAIKDMAPFIVLAFIIGQFIAYFNWTNLGLILAVSGANLLKALNFTGIPLFIGFILLC
                     TFVNLFIGSGSAKWAILAPIFVPMMALLDYHPALTQMLYRIGD"
     gene            1651..2343
                     /locus_tag="VK031_04280"
     CDS             1651..2343
                     /locus_tag="VK031_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLS53166.1"
                     /translation="MAKLLLVEDDTTLAMGIEYSLEKEGYHIDIGRDFSQGKKLVDEN
                     QYDLIILDIGLPDGSGFDLCKYIRRDKTTPIIFLTAKDEEVNVVMGLDIGADDYITKP
                     FRIRELLSRIKAVLRRYDRVNQGNILSSQDLKVHLLESKVMVRGEHIYLTPSEYKLLI
                     IFLNHPKQVLARRSILENLWDIEGDFIDDNSLSVYIKRLREKIELDTSQPEYIKTIRG
                     IGYIWDKDVRGD"
     gene            2336..3568
                     /locus_tag="VK031_04285"
     CDS             2336..3568
                     /locus_tag="VK031_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLS53167.1"
                     /translation="MTSLLRNEEFRSVIVKLIILQVTFALIGFVLVNLFIDDVNKKII
                     ERDMGLVGNIVRDYPELEEEIIPHITKGSTDEDKALGQAILKDYGYSPRIGKNKQPIL
                     KNTSPNIQIFTALLILSSLLPLILVIRGEYKKIYGKINKVSTVAEKVVEGDFSICLNE
                     EGDGDFYILNHQINQMANRLENTLARLNEDKIFLKDTISDISHQLKTPLSSLIMLNEL
                     LMEDEKMEDKTRKKFLEKTKAQLDRMEWLIVNLLKLARLEAGAIEFKREKVFFRDMVD
                     TSMNLLEPQLEGQNVKVGGNHEAFFYGDRDWTEEALINILKNATEYSKGEIEIKLEDT
                     PIFSSIKIRDNGPGINEKDLPHIFERFYKSSSQLKPDSIGIGLNLAKLIVESQEGSLS
                     VRSKKDMGTEFTLTFLKR"
     gene            3693..4385
                     /locus_tag="VK031_04290"
     CDS             3693..4385
                     /locus_tag="VK031_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS53168.1"
                     /translation="MEVVKVENLCKSYGKGNTKVEALKDVNLSINEGEFVAVVGPSGS
                     GKSTLLHLLGGVDRPSSGKVYVDGIDIYNLSEKELAIFRRRKVGFIFQFYNLVPVLTA
                     EENMMLPLLLDQKKVNKNHFEELVHILGLDDRRDHLPNQLSGGQQQRVSIGRALAYNP
                     SIVLADEPTGNLDSKNSKEIVDLLKISVKKYNQTLILITHDLNIAAQADRIISLEDGR
                     VVQDEVMGNENL"
     gene            4372..>5226
                     /locus_tag="VK031_04295"
     CDS             4372..>5226
                     /locus_tag="VK031_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLS53169.1"
                     /translation="MKTYKNITYRYLKGQRNRTLLTIIGIILSVAMVSAIGTIIISAR
                     GALLKEAIRDNGAYHGEFMGIDMEEINKLKNHVDVDKLGIVKNVAAAPVAETTEEERL
                     NFGRDISHRYIDIRAYDENAKDLLPIDLKEGRLPESEDEIVIEYWMRRYLGEDIKVGD
                     KLSLKLGNWIVEDETEAHPDAILKERFEKTGEKEFTIVGFLNRPYVQTGLLVTKGITG
                     LDSKDKLTNQYGAYFTIKDVKNAQDTIENIGKDIGINKAQIKSNFQVLRLYAQSGNPV
                     LDSSLMGIV"
BASE COUNT         1875 a          813 c         1087 g         1451 t
ORIGIN      
        1 atctcctatt ctgtatagca tttgtgttaa agcagggtga taatctaata aggccatcat
       61 aggtacaaat atgggagcca gaattgccca cttggcagat ccactaccga taaataggtt
      121 tacaaaggta catagtagta tgaaacctat aaatagtgga atccctgtaa agtttaaggc
      181 cttaagtaaa ttagcaccag atactgctaa gattaaacct agattagtcc agttaaagta
      241 ggctataaat tggcctataa taaaggctaa gactataaaa ggtgccatat ctttaatggc
      301 catggtcata tattttggta tatctccact acttttaatc tttcctacaa ccttgccata
      361 gggtatggag gtaactaaaa ataggattaa tagtagaggt ataattccag ccaataaggg
      421 tgaaccacta attaaggaac cagtttctgg atttcttaag aagctagact ttggagcagc
      481 tagaattata agtgctgcta aatatatggc tagaccaata ccagcagctt ttaaaccctt
      541 actttctaac tctgaaactt cttgatcttc aatttgtttg ttgccagtat attcacctaa
      601 tctaggttct attatcctat cggaaataag agtaccaaca atggctagaa taactgttga
      661 agttgccatg aaataccagt tggctgttac tggaatttca ggggcaccta caatttgaga
      721 tgcttcgtta gtaattccag ataataagcc atcagtacca gctataaaga agttggcact
      781 aaagcctgca gtagtagctg catagcctaa ggcaatacca gccaaaggat tcctgcctaa
      841 agataaaaat atcatggctg atatggctgg aataataact attgctgcat cagaagcaat
      901 atttccatta ataccaatta acataatcat aaaggtaact aactttggag atgctcctag
      961 tatggtcttt ctcataaatg aggacataag cccaacattt tctgcaagac ctatacctaa
     1021 agccatagta agtaccaagc ctaaaggtgc aaatccagta aagtttgaaa cagcactgga
     1081 cagggcaaaa ttaattcctt ctccagagat taaattcctg gcttcaacta tttcgccact
     1141 ggtagggttc tctacctgaa gtcccacttt actagcaata aaagatatga cagcgatgat
     1201 gacgattaac caaacgaata atataaaaga atgtggaatt ctatttccaa ccctttctac
     1261 ccaggcaaga aagcctttgt tttgatcctt tgctttagca gtcataatta tcctcctttt
     1321 tcatttaatt aagagctaaa gaacaaaatt cacaataatc taacaactgc aactaagttg
     1381 cactcacctc ccttatttta taaatgtaat ccatatatat gttaattaat tgattgttat
     1441 ccttcattat atatgagaaa gaaagggcct tcaacaagaa aaagtgtcga ataatgtcga
     1501 aggccttgtt ttataattat acaatttgat agtgagaaat gcaagaaatc tttttaaact
     1561 ttcatatata ttacaaatta gtaatcttat aagaatagag ataaaggaaa attttatatg
     1621 ttataatgat tttaattagg agggatggct atggcaaaat tacttttggt agaggacgat
     1681 actaccctgg ctatgggtat agagtattct ttagaaaagg aaggttatca tatagatatt
     1741 ggccgggact tttcccaggg gaaaaagcta gtagatgaaa accagtatga tttaataata
     1801 ctagatattg ggctgccaga tggcagtggt tttgatctgt gcaaatatat aagaagagat
     1861 aaaactacac ctataatatt tttaactgcc aaggacgaag aggtaaatgt ggttatgggg
     1921 ctagatattg gggcagatga ttatataaca aagcctttta gaatcaggga actcctatcg
     1981 agaataaagg ctgtccttag aagatacgac agggtcaatc aaggaaatat tttaagttca
     2041 caggatttaa aggttcacct attagagtct aaggtcatgg taagaggaga gcacatatac
     2101 ctgacccctt ccgagtacaa actacttatc atcttcctta atcatccaaa acaagtatta
     2161 gctagaagaa gtattttgga aaacctctgg gacatagagg gagactttat agatgataac
     2221 tctctgtctg tttatataaa aaggttaagg gaaaagattg agttggacac atcccagcct
     2281 gaatatataa aaactataag aggtatagga tatatttggg ataaggatgt aaggggtgac
     2341 tagcttgtta agaaatgaag aatttagatc agtaatagta aagctaatta tcttacaggt
     2401 aacttttgcc cttattggtt ttgttttggt aaatctcttt atagatgatg taaataaaaa
     2461 gataatagaa agggatatgg gcctggtagg caatatagta agggattatc ctgagttaga
     2521 ggaagagata attccccata taactaaagg aagtacagat gaggataagg ccctgggcca
     2581 ggccatattg aaagactacg gctatagtcc tagaatagga aaaaacaaac aacctatttt
     2641 gaaaaataca agtcctaata tccaaatatt tactgccctt ctaatcctat catccctgct
     2701 tcccttaata cttgtaatca gaggagagta taaaaaaatt tatgggaaaa taaacaaggt
     2761 ttccactgta gcggaaaagg tggtggaagg ggatttttct atttgtttaa acgaagaagg
     2821 ggatggagat ttttatatcc ttaaccatca aataaatcaa atggccaata ggctggaaaa
     2881 taccttggca agattaaatg aagataaaat ttttctaaag gacaccatat ctgatatatc
     2941 tcaccaatta aaaacaccct tgtcttcctt aataatgtta aatgaattat tgatggaaga
     3001 tgaaaagatg gaagacaaga cccggaaaaa gtttttggag aaaactaagg cccagctaga
     3061 taggatggaa tggctgatag tcaatttact aaagcttgcc aggctggagg caggagccat
     3121 tgaatttaag agggagaagg tcttttttag agatatggtg gatacttcta tgaacctatt
     3181 ggaaccccag ttggaaggac agaatgtaaa ggttggcggt aaccatgagg cctttttcta
     3241 tggagatagg gactggactg aagaggccct tataaacatc cttaaaaatg caacagaata
     3301 cagcaaggga gaaatagaaa ttaaattgga agacactcct atctttagca gtattaaaat
     3361 aagagataat ggtcctggca taaatgaaaa ggacctaccc catatatttg aaagatttta
     3421 taaaagttcc agccaactga agccagatag tataggcata gggctgaact tagctaaatt
     3481 aatagtagaa tcccaggaag gcagcctaag tgtaagaagt aaaaaagata tgggcacaga
     3541 atttactcta accttcctta aaagatagaa agttataggt caaagggata aagaaatttc
     3601 tactagctta caataatgta atctttatat tcacctgatt gtaagtttca tcctgtaata
     3661 tatacttaga atgaaattca ggaggaagaa atatggaagt tgtaaaagta gaaaatttat
     3721 gtaaatcata tggaaagggc aacaccaagg tagaggccct aaaggatgtt aatctaagta
     3781 taaatgaagg tgaatttgtg gctgtagtag ggcctagtgg ctcaggaaag tctaccctat
     3841 tacatctatt aggtggagta gataggccta gcagtggaaa ggtctatgta gatggaatag
     3901 acatatacaa cctttctgag aaggagctgg ccatctttag aagaaggaag gtaggtttta
     3961 tctttcaatt ctataattta gttccagttt taactgccga agaaaatatg atgcttccct
     4021 tacttttgga ccagaaaaag gtaaacaaga accactttga agaattggtc catatcctgg
     4081 gcttggatga cagaagggat catctaccaa atcaactttc cggtggacag caacaaaggg
     4141 tatccatagg tagggccctg gcctataatc catctatagt cctggccgat gaacctacag
     4201 gaaatttaga cagcaaaaat agcaaggaaa tagtggattt attaaaaata tctgtaaaaa
     4261 agtataatca gaccttgatc cttataaccc atgatttaaa tattgctgcc caggcagata
     4321 ggattataag cctggaagat ggaagagttg tacaggatga ggtgatgggt aatgaaaact
     4381 tataaaaata ttacctatag gtatctaaag gggcaaagaa acaggaccct gcttaccata
     4441 ataggaatta tcctatcagt ggccatggta tcagccatag gcaccattat aatatctgct
     4501 aggggagcct tgttgaagga agccataaga gataatgggg cttaccatgg agaatttatg
     4561 ggaatagata tggaagagat aaataagctt aaaaaccatg tggacgtgga taagcttggg
     4621 atagtgaaaa atgtggcagc tgctcctgtg gcagagacca ctgaagagga aaggttaaat
     4681 tttgggaggg atatttccca taggtatata gacattaggg cctacgatga aaatgctaag
     4741 gacctacttc ccatagacct aaaggaaggt aggctacctg aaagtgaaga tgagatagta
     4801 atagaatatt ggatgaggag atatttagga gaggatataa aagtaggaga taagctaagc
     4861 ttaaaactgg gaaattggat agtggaggat gaaactgagg cccacccaga tgctatcctt
     4921 aaggaaaggt ttgaaaaaac aggggaaaaa gaatttacca tagtaggatt tttaaatagg
     4981 ccttatgtac aaacaggatt attggtgacc aagggaatta caggcttgga ttcaaaggat
     5041 aaacttacca atcaatatgg ggcttatttt actataaaag acgtaaaaaa tgcccaggat
     5101 actatagaga atattggaaa agacatagga ataaataagg ctcagatcaa aagcaatttc
     5161 caggtactaa gactctatgc ccaaagtgga aatccagttc ttgatagctc tcttatgggg
     5221 attgta
//
LOCUS       DATGSI010000283         4380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_443593, whole genome shotgun sequence.
ACCESSION   DATGSI010000283 DATGSI010000000
VERSION     DATGSI010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443593"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VK031_04300"
     CDS             <1..249
                     /locus_tag="VK031_04300"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HLS53170.1"
                     /translation="IEGAYNDIVTKVGISRQHAKNMVENQEVLMSQLEQRRESTSGVS
                     IDEEVTNLIKFQTAYDANARVISVLSEMLDVLINRTGV"
     gene            253..1152
                     /gene="flgL"
                     /locus_tag="VK031_04305"
     CDS             253..1152
                     /gene="flgL"
                     /locus_tag="VK031_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HLS53171.1"
                     /translation="MRITNKTLTNTFLRNLSRNLEQMQKYQDQLSSGKEVSRPSDNPM
                     LVSKIMFLKNNILQNKQYNSNINDTIGWVETQDTALNDMTATLNRIRDLIIYGANGSL
                     SDTDRAAIKDEVDMQIDQLADILNTNFDGRYIFAGQETLTRPFEKGENGLEYLGDDSN
                     IFREISHGVEIGLITSGSSLTYTEDANTEDLGELLGKIVEALDTGDMESLAGELLSDI
                     DKHIDTVIRVRSSIGAIHNRLEAAKDRNESENMNLTKLLSDREDIDVAEKYMEYSVMA
                     VVYQASLSVGTKILQPSLLDYLN"
     gene            1166..1612
                     /gene="fliW"
                     /locus_tag="VK031_04310"
     CDS             1166..1612
                     /gene="fliW"
                     /locus_tag="VK031_04310"
                     /inference="COORDINATES: protein motif:HMM:NF009793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HLS53172.1"
                     /translation="MIIKTKYLGEVEIDKEKIISFPKGILGFHNHKEFVILDIQKNNY
                     FKFLQDIHNQYVAFLIINPWSFYKDYDIEVADEKLMDIKINPKEDNQIAVYNIVTLGS
                     SLSNSTVNLLAPIVINIKDRLGKQFILDDSPYSTKHRLPIAGEDTC"
     gene            1606..1836
                     /gene="csrA"
                     /locus_tag="VK031_04315"
     CDS             1606..1836
                     /gene="csrA"
                     /locus_tag="VK031_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HLS53173.1"
                     /translation="MLILNRKANESIMLDDKIEIRILEVQEGKVKLGIEAPKEVTILR
                     KEVYDEVIEANKKSLDIGQDKNILEELNKFNK"
     gene            1986..2849
                     /gene="hag"
                     /locus_tag="VK031_04320"
     CDS             1986..2849
                     /gene="hag"
                     /locus_tag="VK031_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin Hag"
                     /protein_id="HLS53174.1"
                     /translation="MRINQNISAMNTYSRLTAANASKSKSLEKLSSGLRINRAGDDAA
                     GLAISEKMRGQVGGLKQAVRNAQDGISLIQTAEGALTETHSMLQRMRELAVQAANETN
                     TGEDIQQIQDEFNQLAVEITRVATDTQFNTKAVFGGVVDGEVVGDVSDDAKAEFTFQI
                     GANDNQTMKIEIGRMDAIALAVGEEAEGELTAIDVTGENILSIIDEAIDKVSSQRASL
                     GAAQNRLEHTINNLTTTAENLSAAESRIRDVDMAEEMMEFTKNNILSQAATAMLAQAN
                     QMPMSVLQLLQ"
     gene            2914..3282
                     /locus_tag="VK031_04325"
     CDS             2914..3282
                     /locus_tag="VK031_04325"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HLS53175.1"
                     /translation="MIYITRPVTETHKTVPKTTDKSLDNNLRERRPMSNIGRDMGTKE
                     RDDYHKDELEKAIEETNKIIFRDNSRFEFKLHEGTGRMMVKLIDKETDEIIKEIPPEK
                     ILDLVASIWDLVGILVDERG"
     gene            3285..>4380
                     /gene="fliD"
                     /locus_tag="VK031_04330"
     CDS             3285..>4380
                     /gene="fliD"
                     /locus_tag="VK031_04330"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HLS53176.1"
                     /translation="MAPISFIGSYSGIDQNTIEQLLEVERLPLKQLSNKKDNITEKQN
                     AWRDINTRLNSLFEKIKALQNQDTFLSKIATSSNEDYVTMSPSKNAASGIYRISVGQL
                     ATNTNIIGGKVLQVGQTTTDQLGLKGSFTMANEEGKEVMVEVNETDSLRDIVNKINKS
                     LYFENEDSKGEDIGIKASIIDGRIVLTDEKSGNRNISLIDGGEGLLSDLALDENNRKV
                     TEGQQAQFTINGVQVESDTNSVTDVLDGVTINLNKTHEEGQYDTVTIGIDEEKAVKAL
                     KEFVDQYNSTMNFIEDKLKAGDPNSPGSRGPLAGDSSLMRLQSSLRQMVTSSLGGLDT
                     KITDISQLGVKTQDRFGQLTFDENKFKQALR"
BASE COUNT         1693 a          687 c          916 g         1084 t
ORIGIN      
        1 atagaaggcg cctacaatga tatagtaacc aaggtaggta taagtaggca acacgctaaa
       61 aacatggtgg aaaaccaaga agttcttatg agccagctgg aacaaaggag agaatcaacc
      121 tcaggtgtat ccatagatga agaggtaacc aacctgatta agtttcaaac tgcttatgat
      181 gccaatgcta gagttatatc agtattatca gaaatgttag atgttctaat aaataggact
      241 ggggtgtagt ttatgagaat cacaaataaa accttaacca atactttttt aaggaatctt
      301 tcaaggaatc tagaacaaat gcaaaaatac caggaccagc tatcctctgg taaggaagta
      361 tctagacctt cagataatcc tatgctggta tcaaagatta tgtttcttaa aaacaatatt
      421 ttgcaaaaca aacaatacaa ttcaaatata aatgatacca taggttgggt agaaacccag
      481 gatacagccc taaatgatat gacagccact ttaaatagga taagggactt aataatctac
      541 ggtgccaatg gttccctatc tgatactgat agggcagcca taaaggacga agtagatatg
      601 caaatagatc aattggcaga tatcctaaat acaaactttg acggtagata tatctttgca
      661 ggccaagaaa ccttaaccag gccctttgaa aagggggaaa atggattaga atatctgggt
      721 gatgatagta atatatttag agaaatctcc catggtgtag aaataggcct aataaccagt
      781 ggtagcagcc tgacctatac agaagatgcc aatacagagg atttaggaga acttttagga
      841 aaaattgtag aggctctaga tacaggtgat atggaaagcc tggccggtga gcttctttct
      901 gatatagaca aacacataga tacagtaatc agagtaaggt ctagtatagg ggccatccac
      961 aataggctgg aagcagccaa ggatagaaat gaatcagaga atatgaatct aacaaaacta
     1021 ttatctgaca gagaagatat agatgtggca gaaaagtata tggaatatag tgtaatggca
     1081 gtagtatatc aagcctccct atctgttggt acaaaaatcc tacagccaag cctattggat
     1141 tatttaaact aatgggaggt ttaaaatgat aataaaaaca aagtatctag gtgaagtgga
     1201 aatagataag gaaaaaatta taagttttcc taaaggtatt ttaggttttc ataaccacaa
     1261 ggaatttgta atcctagata tccaaaaaaa taactatttt aagtttttac aagatataca
     1321 caatcaatat gtggcctttt taattataaa tccatggagc ttttacaagg actacgacat
     1381 agaagtggca gatgaaaaac taatggatat aaaaataaat cctaaggaag ataaccaaat
     1441 agcagtatat aatattgtca ctctagggag tagcttaagt aatagtacag tcaatcttct
     1501 ggcccccata gttataaata taaaagatag gttaggtaaa caatttatcc tggatgattc
     1561 accttattca accaagcata ggttgcctat agcaggtgaa gatacatgct aattttaaat
     1621 agaaaggcca atgaaagcat tatgctagat gacaagatag aaatccgcat actagaagtc
     1681 caggagggta aagtaaagtt aggcatagaa gccccaaagg aagtaaccat actaagaaaa
     1741 gaagtctacg atgaagtaat tgaagccaac aaaaaatctt tagacatagg tcaagataaa
     1801 aatatattag aagaactaaa taaattcaac aaataagaga gcctaggctc tcttatttat
     1861 ctttcaaaaa acttttaaaa cactattaaa ctttttctta tccttccgat aaagataata
     1921 agaagtcata tggccatact tcttaaggca aggatgccaa ataaaaaatt caaggaggaa
     1981 aagatatgcg tattaatcaa aatatttcag ccatgaacac ttactcaaga ctgacagcag
     2041 caaatgcgtc aaagagtaaa tctttagaaa aattatcatc aggattaaga attaacaggg
     2101 caggagatga cgcagcagga ctagccatct ctgaaaagat gagaggacaa gttggaggac
     2161 ttaagcaagc tgtaagaaat gcccaagacg gtatttccct aatccaaact gcagaagggg
     2221 ccctaacaga aactcactcc atgttacaaa gaatgaggga gttagcagtt caagctgcaa
     2281 acgaaacaaa tactggtgag gatattcaac aaattcagga cgagtttaac caattagcag
     2341 tagagataac aagagttgct acagataccc aatttaatac aaaagccgta tttggtggag
     2401 ttgtagatgg tgaagtagtt ggagatgtaa gtgatgatgc taaagctgaa ttcaccttcc
     2461 aaataggtgc caatgataat caaactatga agattgagat tggaagaatg gatgcaattg
     2521 cattggctgt tggagaagaa gctgaaggag aactaactgc aattgatgta actggagaaa
     2581 atatactcag cataatagat gaagccatcg ataaagtatc cagccaaaga gcatctttag
     2641 gtgctgctca aaatagacta gagcatacaa tcaacaattt aactaccaca gctgagaacc
     2701 taagtgcagc tgaatctaga atcagagacg tagacatggc agaggaaatg atggagttta
     2761 ctaaaaacaa catcttaagc caggcagcta cagccatgct agctcaagca aaccaaatgc
     2821 caatgtcagt tttacaacta ttacagtaaa atttataatg gccggtgggg taaccgccgg
     2881 ccatttttac aaataatctt ggggtgatgg tagatgattt atattacaag accagtaact
     2941 gaaacccaca aaactgtacc aaaaactaca gataaatcct tagataataa cctgcgggaa
     3001 agaaggccta tgtcaaatat aggcagggac atggggacaa aggaaagaga tgattatcat
     3061 aaggatgagt tggaaaaggc catagaagaa accaacaaaa taatttttag agataatagc
     3121 cgttttgaat ttaagctaca tgaaggcaca ggccgcatga tggtaaagct aatagataag
     3181 gaaacagatg aaatcattaa ggaaatacca ccagagaaaa tactagacct agtggccagt
     3241 atttgggatc tggtaggtat cttggtagat gaaaggggat aaaaatggca ccaattagtt
     3301 tcataggttc ctattcagga atcgatcaaa acacaattga acaattatta gaagtagaaa
     3361 gacttccttt aaaacaatta tcgaataaga aagacaacat aaccgagaaa caaaatgcct
     3421 ggagggatat aaatactagg ctaaatagtc tttttgaaaa gataaaggca ctacaaaatc
     3481 aagatacctt cttaagtaaa attgccacat cttccaatga agattatgtt actatgtctc
     3541 catcaaagaa tgcagcctct ggaatctata gaatcagtgt tgggcagttg gctaccaaca
     3601 ccaacataat cggtggaaaa gttttacaag taggacaaac aactaccgac cagctaggcc
     3661 ttaaaggctc ctttaccatg gccaatgaag aaggaaaaga ggttatggtt gaagttaatg
     3721 aaactgatag cttaagagac atagtaaata agattaataa aagcctttat tttgagaatg
     3781 aggattcaaa gggtgaagac ataggtataa aggccagcat aatagatgga agaattgtcc
     3841 ttactgatga aaagtctggt aatagaaata taagcctaat agatggagga gaggggcttt
     3901 tatcagatct agccctggat gaaaataata gaaaggttac tgaaggccag caggctcagt
     3961 ttaccataaa tggggtacag gtagaaagtg atacaaattc tgttacagat gttttagatg
     4021 gggtaactat taatttaaac aagacccatg aagaggggca gtatgatact gttaccatag
     4081 gcatagatga ggaaaaggca gttaaggccc tgaaagaatt tgtagatcaa tataattcta
     4141 ccatgaactt tattgaagat aagctaaagg caggagatcc taatagccct ggtagcaggg
     4201 gacctttggc aggagacagt agtttaatga ggctacagtc aagtttaaga caaatggtaa
     4261 cttctagcct gggagggctg gatacaaaaa ttaccgatat atcccagtta ggggtaaaga
     4321 ctcaagatag gtttggccaa cttacctttg atgaaaataa gtttaagcag gccctaaggg
//
LOCUS       DATGSI010000284         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_301231, whole genome shotgun sequence.
ACCESSION   DATGSI010000284 DATGSI010000000
VERSION     DATGSI010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301231"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VK031_04335"
     CDS             complement(<1..228)
                     /locus_tag="VK031_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HLS53177.1"
                     /translation="MTFKLTDKVTWVGKIDWELKTFHGEEYSTDRGSSYNAYLIRDEK
                     TVLIDTVWEPFAKEWLEKLKEEIDLNEIDYIV"
     gene            complement(315..875)
                     /locus_tag="VK031_04340"
     CDS             complement(315..875)
                     /locus_tag="VK031_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HLS53178.1"
                     /translation="MEAIRIMVEEHENIRRMLKVIRRVSYEIMTLGDFDIEDVGKIID
                     FVRNYADGHHHAKEEDILFDTMDKSLEKLSKSGAIKGMYIEHDLGRLYMGSLEKAIDS
                     FKAGDDEARLDIIANAISYSDLLDRHIQKENTALYKFAENMLDESSKEYIEAKCQDVE
                     KRAEEKGIQDKYLNLLEELEEKYKIN"
     gene            complement(890..1570)
                     /locus_tag="VK031_04345"
     CDS             complement(890..1570)
                     /locus_tag="VK031_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLS53179.1"
                     /translation="MNCNCHNRDGRNCIELVPIFSNLNEEEMLEVARITTDRKFHKGQ
                     MIYMAGDKGEKLYVIHKGKVKITRYTETGKEQVIRILGPGEFMGELSLFSSTPLKDNG
                     EALEETTMCIIDGNQFKILMKKYPDIGFKIMKELSHRLEKAESLIENISLHSVEKRLA
                     TTLLNMANRDGEVKLKMSKRDLASHIGMSQETLSRKLSAFQELGIIRLIGHRRIIILN
                     RAALEEIE"
     gene            complement(1739..2764)
                     /locus_tag="VK031_04350"
     CDS             complement(1739..2764)
                     /locus_tag="VK031_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HLS53180.1"
                     /translation="MKMFKSDNASGIHPKILQAIIDANQGHEHPYGNDRLTKRAKEEM
                     SKLFGKDVDTYFVTTGTAANVIGLASLLRPYEAIVCTDTAHINTDEGGAFELFSGSKI
                     LYTANKNGKIDINDIKKHFVSIGDEHRSQPRIISISQTTETGSLYTVEEIKRLADLAH
                     QNNMLLHVDGARIANACVALNVSIKEMITDTGVDLLSFGGTKNGMMVGEAIVSFNKDL
                     SKNLKFYRKQGMQLLSKMRFISAQFLAYLEDGLWLENAENANKMGQYLKNELSKLPGI
                     KVKDDMKTNIIFAHVGKYIIDELQDKFGFYLFDQETNLIRLVTSFDTKKEDVDSLLEA
                     IKKLEER"
BASE COUNT          843 a          639 c          421 g         1185 t
ORIGIN      
        1 tactatatag tctatttcat ttaggtctat ctcttccttt aatttttcca accattcctt
       61 agcaaagggc tcccatactg tatcaataag tactgttttt tcatccctga ttaagtaggc
      121 attatatgag gaacctctat cagttgaata ttcttctcca tggaaggttt taagctccca
      181 gtcaattttt cctacccagg ttaccttatc tgtcagttta aaagtcattc tattacctcc
      241 ttttaatttt gaaagatact tatcctataa tttaccccta agttggtaaa ttataggata
      301 attgtcggaa tattttaatt aattttatat ttttcttcca attcttccag tagatttaaa
      361 tatttatcct gaattccttt ttcttctgcc cttttttcca cgtcctggca cttggcttct
      421 atatattcct tgctactttc atccaacata ttttcggcaa atttataaag tgctgtgttt
      481 tctttttgga tatgcctatc cagtaggtca ctataggaaa tggcattggc tattatatct
      541 agcctggcct catcatcccc tgccttaaag ctatctattg ctttttctaa actccccata
      601 tacaatctgc ctaaatcatg ctctatatac atgcctttaa tggccccaga cttggatagt
      661 ttttctaaac ttttatccat ggtatcaaat aaaatgtctt cttccttggc atgatggtgt
      721 ccatcagcat agttcctaac aaagtctata atcttaccta catcttctat atcaaaatct
      781 cccagggtca tgatttcata agataccctt ctgattacct ttaacatcct tctaatattt
      841 tcatgttctt caaccataat tctaatggct tccattttat caccccattt tattctattt
      901 cttccaaggc tgccctattt aatattatga tccttctatg acctattaac ctaataattc
      961 ctaattcctg aaaggccgat agcttcctgc ttaatgtttc ctgactcata cctatatggg
     1021 aggccaaatc ccttttactc atttttagct taacctctcc gtccctgttg gccatattaa
     1081 gcaaggttgt ggccagtctt ttttccactg aatgtaggct aatattttct attaggcttt
     1141 ctgccttttc taacctatgg cttaattcct tcattatttt aaaacctata tctggatatt
     1201 ttttcattag tattttaaac tggttaccgt caataataca catggtagtt tcctccaagg
     1261 cctctccatt atctttcaag ggtgttgagc taaataaaga cagttccccc ataaactctc
     1321 caggtcctaa aatcctaatt acctgctcct tgcctgtctc tgtatatctg gtaatcttta
     1381 ccttgccctt atgaattaca tatagctttt ctcccttgtc tccagccata tatatcattt
     1441 ggcccttgtg aaattttcta tctgtggtaa tccgggccac ttctaacatt tcttcttcat
     1501 tcaaattact aaatatgggg accaattcta tacaatttcg cccatcccta ttatggcaat
     1561 tacagttcat ttttatccct ccctgcctat atccttatat ggttattata cccataggcc
     1621 ctggatccac actgcctatg gaagtagtgt caacctatat aatatatttt ataattatgg
     1681 tctattaaaa actttgacct aggtcaaaaa tataaaaaaa agaagggtaa tctttaaatt
     1741 acctttcttc cagttttttg atggcctcta aaagactatc tacatcttcc ttttttgtat
     1801 caaaggaggt tacaagcctt attaaatttg tctcctgatc gaaaagataa aagccaaatt
     1861 tatcctgtag ttcatcaatt atatattttc ccacatgagc aaatataata tttgttttca
     1921 tatcatcttt aaccttaatt cctggtaatt tagatagctc attttttaag tactggccca
     1981 ttttattagc attctctgca ttttccagcc aaagcccatc ttctaagtag gccaaaaatt
     2041 gggcagatat aaacctcatt ttagataaaa gttgcatgcc ctgctttcta taaaacttta
     2101 agtttttact taagtcctta ttaaaagata caatggcttc ccctaccatc atgccatttt
     2161 tagtgccacc gaaggataat aaatctactc ctgtatcagt gatcatttct ttaatactaa
     2221 catttaaagc cacacaagca ttggctattc tggccccatc cacatgtaat aacatattat
     2281 tttgatgggc caagtctgcc aaccttttta tctcctctac tgtatagagg ctacctgttt
     2341 ctgttgtttg tgatatggaa attatcctag gctggctcct atgctcatct ccaatggaga
     2401 caaaatgttt tttaatatca tttatatcta ttttgccatt tttattagct gtgtataaga
     2461 tttttgaacc actaaataat tcaaaggccc caccttcatc tgtatttata tgggctgtat
     2521 ctgtacatac aatggcctca tagggtctta ataggctggc tagtccaatt acattggctg
     2581 ctgtgcctgt tgttacaaaa taggtgtcca catccttacc aaaaagtttt gacatttctt
     2641 ccttggccct tttagttagt ctgtcatttc cataaggatg ttcatgacct tgatttgcat
     2701 ctattatggc ctgtaatatt tttggatgta tgcctgatgc attgtctgat ttaaacattt
     2761 tcatcttgtc catctctcct tttcatagct atattatata ataaatttag gtagatttgc
     2821 atggtttatg ggtaatttat agtcaagtcg cctttagttt tatcaagtct gactcaggcc
     2881 atggcagggt tctaaccttt taaattttaa aataaaaata taaataggaa agcttttgtg
     2941 ttataataaa ttaactaatt tagtgtgtaa aggagtaggc ttatggcaaa atatttgctt
     3001 attataataa ttggctattt gtttggatgt ttacaatggt cctatatctt aagtaagact
     3061 ttaaagaagc aggatattag aaagctag
//
LOCUS       DATGSI010000285         1767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_398221, whole genome shotgun sequence.
ACCESSION   DATGSI010000285 DATGSI010000000
VERSION     DATGSI010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398221"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1767
                     /locus_tag="VK031_04355"
     CDS             <1..>1767
                     /locus_tag="VK031_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53181.1"
                     /translation="EATNKRLKRLSSTGISQVGKSFQGLRLRMDKVNKSFARAPFSSV
                     TPKIGRFTNALGQSRTSLSRFSKSSGTANFAVLGLSQGLQDLPFGFIGIQNNIPMVIR
                     SFSQLRQETGSTKAAFKGLGNALLGSGGLFLGTSLLISGITLLTQKYGSLGNAWDVLT
                     GKASALYNKQKQLNKAFENASGLKSYLETSIRLGVELDSVSSGVVSQSKFLKDYNKII
                     KDSSEKFDNFNAAQQDFLNNNDNIIKAYVKQATSQQLIQKAAGLTTKLIQSQNKEGKK
                     FVGFWQSSWINIKSQISSLFNNPVGYSDAYKRFNKSFKKAGISNKKEEISDFKEAIKD
                     AMKDAGDSYKKFLKLLKENNLYKYFFGGDTNGGGGNPITNNIKTISDIIADMQKEMQQ
                     LSARAKVFGKSISQVKIEKIGILKEYMKTLANSLLPKSIEKAKELGNEISKIRSSLNF
                     EGVSPIEGKLPFAQTPIDQNKINEATSKVRGTARINKLEKATKDWGNTLSIVNGLFTD
                     IFQNAAGGQSVFEALARAAERMAVKLAAAAASAALISAISGGATSFIGGLGKILGFAN
                     GGFVSSPTLAVVGDDNTGGEWI"
BASE COUNT          621 a          249 c          390 g          507 t
ORIGIN      
        1 tcgaagccac aaataaaaga ctaaagagat tatctagtac gggaattagt caggtaggga
       61 agtccttcca agggcttagg ttgcgtatgg ataaggtaaa taagtctttt gcaagagcac
      121 ccttctctag cgttactcct aagataggtc gcttcactaa cgcgctcgga caatccagga
      181 catcattaag tagattttca aagtctagcg gtactgccaa tttcgctgtt ttaggattat
      241 cacaaggact acaggattta ccctttggat ttataggtat tcagaataat atacctatgg
      301 tgatacgttc tttttctcaa ttaaggcagg aaacaggctc cactaaagcg gcatttaagg
      361 ggttaggtaa cgctttactg ggttcagggg ggttgttttt gggtacatcg ttacttatct
      421 cgggtattac attattgacg caaaaatacg ggagtctagg gaatgcgtgg gatgttttaa
      481 cagggaaagc cagtgcttta tataataaac aaaaacagct aaataaagca ttcgagaatg
      541 cgagtggatt aaaatcatac ctagaaacat ctattagatt gggggtggag ttagattctg
      601 ttagtagtgg agttgtttct caaagtaaat ttctaaagga ttataataaa ataattaaag
      661 attcctctga aaagtttgat aatttcaatg cagcccaaca agacttcctt aataataatg
      721 ataatattat taaagcttat gtaaaacagg caacatctca acaattaata cagaaggcgg
      781 ctggattaac gaccaagtta atacaatctc aaaataagga gggtaagaaa tttgttggat
      841 tctggcaaag tagctggatt aacataaagt cccaaatttc atcattattc aataaccctg
      901 ttggatatag tgatgcatac aaaagattta ataagtcttt taaaaaagca gggatatcta
      961 ataagaagga ggagatttct gactttaagg aggcaataaa ggatgcaatg aaagatgcag
     1021 gggattcata taaaaagttc ttgaagctat taaaagaaaa taacctatat aaatattttt
     1081 tcggtgggga tactaatgga ggaggcggta atcctataac aaataatata aagactattt
     1141 ctgatattat tgcagatatg caaaaggaga tgcagcagtt atctgcaaga gctaaagtat
     1201 ttggtaaatc tatttcacag gttaagatag agaaaatagg gatattaaaa gaatatatga
     1261 agactttagc taatagtctt ttaccaaagt ctattgagaa agctaaagaa ttgggcaatg
     1321 aaattagcaa gatacgctca agtttaaatt tcgagggagt atcacctata gaaggcaaat
     1381 taccattcgc gcaaacacct attgaccaaa ataaaattaa tgaagcaaca tctaaagtta
     1441 gaggtactgc taggattaat aaactggaaa aagcgacgaa ggactgggga aacactttaa
     1501 gtattgttaa tggtcttttt actgatatat ttcaaaatgc agctgggggg caaagtgttt
     1561 ttgaggcttt agctagagcg gcagaaagaa tggcggtaaa attagctgct gctgctgctt
     1621 ctgctgcttt aattagcgca atctctgggg gagcaacatc ctttatagga ggattaggta
     1681 aaatattagg ttttgcaaat gggggatttg tatcaagtcc tacattagca gtagtggggg
     1741 atgataatac gggaggagaa tggatat
//
LOCUS       DATGSI010000286         1711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_66502, whole genome shotgun sequence.
ACCESSION   DATGSI010000286 DATGSI010000000
VERSION     DATGSI010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66502"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /gene="pepD"
                     /locus_tag="VK031_04360"
     CDS             <1..1004
                     /gene="pepD"
                     /locus_tag="VK031_04360"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HLS53182.1"
                     /translation="ETGMDGAYALTGENLSGKILLNLDSEEEGVFLASCAGGADISTS
                     FKVEKEDGAGVGLEIMVSGLKGGHSGMEIIKQRGNAIKILARLLYQCKTEENIRLAHM
                     AGGTKSNAIPAEARAKIWVEDIKGVKELISKLAAEIKAEYMVEEEELVIKTREIDIEK
                     VYTKDLSDNIIDYLMMVPDGVQYMSKDIEDLVLTSLNNAIIKEEGSYIRIESSLRSSM
                     ASSLEEIANRLMVMAKRSNATAKLKGQYPAWQYEEESKIRDICVNLYEEMFNERATVT
                     AIHAGLECGILKKILPDVDMISFGPNLYDVHTEKEHLSIPSVNRMWKFLVRLLEELK"
     gene            1056..1403
                     /locus_tag="VK031_04365"
     CDS             1056..1403
                     /locus_tag="VK031_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtrH family sporulation protein"
                     /protein_id="HLS53183.1"
                     /translation="MFVLSNFFVSAIQNFFIAFGVVVGASLFAGLGAVITGQPPLRTM
                     TQLADSMKIWGMAVALGGTFSSFAAINKGLFQGEIKTIIKQVIYIFVALLGSNAASTF
                     IQLIQRCGQIWSK"
     gene            1391..>1711
                     /locus_tag="VK031_04370"
     CDS             1391..>1711
                     /locus_tag="VK031_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53184.1"
                     /translation="MVKIKREKASHILYLITGLISGIIIGSLSLLLMVSYRLEEYHKE
                     IVSLENKIEESRAKLEKLEEAGEKPYLSLQDIKVHLDFQGDEIAKIHIEKAIKEKYIS
                     LLGKD"
BASE COUNT          613 a          245 c          390 g          463 t
ORIGIN      
        1 aggaaacagg catggatggg gcctatgcct taactggaga gaacctatca ggaaaaatac
       61 ttttaaacct agattctgaa gaggaaggag tatttttggc cagttgtgct gggggagcag
      121 atataagcac aagttttaaa gtagaaaagg aagatggggc tggagtgggc ctagaaatta
      181 tggtttcagg cttgaaaggt ggccactctg gtatggaaat tattaagcag aggggtaatg
      241 caattaagat tttagctagg ctcctttacc agtgtaagac agaggaaaac ataagattag
      301 cccatatggc aggaggtact aaatccaatg caatacctgc tgaagcccgg gcaaaaatat
      361 gggttgaaga cataaaaggc gtaaaagagc taatttccaa gctggcagca gaaatcaaag
      421 ctgaatatat ggtggaagaa gaggaacttg ttattaagac tagagaaata gatatagaaa
      481 aggtttatac aaaggactta agcgacaata taatagacta cttaatgatg gtgccagatg
      541 gagtccaata tatgtctaag gatatagagg atttagtatt gaccagctta aacaatgcca
      601 ttataaaaga agaaggttct tatataagga tagaaagctc tcttagatct tccatggcca
      661 gttcactgga ggaaatagcc aataggttga tggtaatggc taaaaggtct aatgctactg
      721 caaaacttaa aggacaatat ccggcctggc aatatgaaga agaatctaaa attagagata
      781 tatgtgtaaa cctatatgag gaaatgttta atgaaagggc tactgttact gccattcatg
      841 cagggttaga atgtggtata ttaaagaaga ttttaccaga tgtggatatg ataagttttg
      901 gaccaaactt gtatgatgtg catacagaaa aggagcacct aagtattcct tctgtaaata
      961 ggatgtggaa gtttctagta agattattag aagaattaaa ataggtaaac ataaagacaa
     1021 tacaaaaatc atatcttgta aaaagaagat aggatgtgtt tgtattgtct aatttttttg
     1081 tttcggctat ccaaaacttc ttcattgctt tcggtgtagt agtaggggcc agcctatttg
     1141 caggccttgg tgccgtaata acaggacagc ctcccctaag gacaatgaca cagttggcag
     1201 actcaatgaa aatttggggg atggcagtag ccctaggcgg taccttttct tcatttgcag
     1261 ctataaataa ggggcttttc cagggagaga taaaaacaat tataaagcaa gttatctata
     1321 tttttgtggc tctactaggc tctaatgctg cctctacctt tatccaatta atccaaagat
     1381 gtggtcaaat atggtcaaaa taaaaaggga aaaagcatcc catattttgt atcttattac
     1441 aggcttaata tcagggatta taataggcag cctatcctta ttacttatgg ttagctatag
     1501 attagaagag tatcacaagg aaattgttag ccttgaaaat aaaatagaag aaagcagggc
     1561 aaagttggaa aagttagaag aggctggaga aaagccttat ctgtccttac aagatataaa
     1621 agtccaccta gactttcagg gagatgaaat agctaaaatt catatagaaa aggctattaa
     1681 agaaaaatat atatccctgc tgggaaaaga t
//
LOCUS       DATGSI010000287         1026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_635385, whole genome shotgun sequence.
ACCESSION   DATGSI010000287 DATGSI010000000
VERSION     DATGSI010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635385"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VK031_04375"
     CDS             complement(<1..519)
                     /locus_tag="VK031_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLS53185.1"
                     /translation="MAKTRILVVDDEKNITELIRMNLEAEGYEIDLAYTGMEAIIKTD
                     SFKPDLILLDLMLPDIDGLQVCQMIRLNEKTQNIPIIMISARSEESNKVEGLSVGADD
                     YITKPFGLKELEARIRTVLRRVDGIFDDSRMSIDTNIVKYRDMVMDINKYQLRKENTL
                     IDMTPTEFKIMKL"
     gene            complement(699..>1026)
                     /locus_tag="VK031_04380"
     CDS             complement(699..>1026)
                     /locus_tag="VK031_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53186.1"
                     /translation="TNVFSRRTEENRSLSFNILSDSECGFMEQIEKICQDHSLYIKDI
                     HIFCAEHDSDRRKSGKLGVKVSVRSSKKKSIEDFNLVLEEISEIDGVLEVYVNNTNIY
                     SEKETI"
BASE COUNT          289 a          226 c          116 g          395 t
ORIGIN      
        1 taatttcatt atcttaaact cggtgggcgt catgtctatt aatgtgtttt cttttctcaa
       61 ttggtattta tttatatcca tcaccatatc tctatacttc acaatattgg tatctatact
      121 catcctgcta tcatcaaata tgccatcaac tcttcttaat actgtcctta tccttgcctc
      181 tagctccttt aatccaaaag gcttcgttat ataatcatcg gctcctacac ttagaccttc
      241 taccttattg gattcctctg atctagctga aatcattatt attggaatgt tttgggtttt
      301 ttcatttaac ctaatcattt ggcaaacctg caacccatca atatcgggaa gcattaagtc
      361 taacaaaata aggtctggtt taaatgagtc tgtttttatt atagcttcca taccagtata
      421 agctaagtct atctcatatc cttctgcctc tagattcatt cgtataagtt ctgttatatt
      481 tttttcatca tccacaacaa gtattctagt tttagccatt ataccgcccc ttccaaccaa
      541 cttgttctac tatattacat cataccattc catgttgtct aagacaataa aacgataaat
      601 cttcggtttt tttataatta aaaaggaata gccatcttgt cccagttaga atttcgaacc
      661 tatactaact ggagccttag ctattcctta ctatttattc atatggtctc tttctcacta
      721 tatatattag tattgttaac gtatacctca agtactccat ctatctcaga tatttcttcc
      781 aatactaaat taaaatcttc tatgcttttc ttcttcgacg atcttacaga aacctttact
      841 cctagtttac cagattttct cctgtctgaa tcatgttctg cacagaatat atggatgtct
      901 ttaatatata aactatgatc ctggcagatc ttctcaatct gttccataaa tccacactca
      961 ctatcagaaa gtatattaaa agagagcgac ctattttctt ctgttctcct actaaataca
     1021 ttggtc
//
LOCUS       DATGSI010000288         3624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_493063, whole genome shotgun sequence.
ACCESSION   DATGSI010000288 DATGSI010000000
VERSION     DATGSI010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493063"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VK031_04385"
     CDS             <1..1078
                     /locus_tag="VK031_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HLS53187.1"
                     /translation="VSNLWLISRLIGMILAIMVYFKLGPSWIYSEDTGGLILYDLIQG
                     LFAIFLFAGFLLPFLTDFGLLEFVGALLTPIMRPIFGLPGRASIDCIASWVGDGTIGV
                     ALTNKQYEEGYYTNKEASIIATTFSAVSITFCLVVLGQVDLAHLFGPYYLTVAVAGIV
                     AAIIVPKIPPLSRKSNDYYKGIKKDKGENAPEGYTNISWGLELALKKADESFKPREFI
                     ANGTKSVLDLWLGVLPIIMAFGTIALIIAEFTPVFQWLGMPFIPILKLLRVPYAVEAS
                     QTIMVGFADMFLPSVIGASIPSEMTRFIIASLSVTQLIYLSEAGAVMLGAKMDISLKD
                     LFIIFIERTLITLPIIVLAANIIF"
     gene            complement(1110..2126)
                     /gene="namA"
                     /locus_tag="VK031_04390"
     CDS             complement(1110..2126)
                     /gene="namA"
                     /locus_tag="VK031_04390"
                     /EC_number="1.6.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF010047.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH dehydrogenase NamA"
                     /protein_id="HLS53188.1"
                     /translation="MKFATKFKIKDIELKNRIVMPPMCMGVSDDKGMVKDFHKLHYAT
                     RAVGGVGLIISESIAIEPNGRISNKDLGIWSDDHIAGLKDLVDKVKSYGSKTAIQLSH
                     AGRKCSVAGESLVAPSVLRHSDKYEKPKEMSQDDIRNMVKSFKEAARRANEAGFDIVE
                     IHAAHGYLIHQFLSPITNKRTDEYGGSLEGRVRFLKEILEEISKVWPKDKPIFIRVSA
                     TDYVDGGMDEKEMVKIINQVKDYIDVVDVSTGGLVNVKIKSYPGYQAPYAEIIKDKCN
                     IPTITAGQIRNFEQIEEILSNGRADLVALGRKLLNEPYFVLNIAHENKLNIDYPKPYM
                     AVYH"
     gene            complement(2182..3198)
                     /gene="namA"
                     /locus_tag="VK031_04395"
     CDS             complement(2182..3198)
                     /gene="namA"
                     /locus_tag="VK031_04395"
                     /EC_number="1.6.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF010047.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH dehydrogenase NamA"
                     /protein_id="HLS53189.1"
                     /translation="MKTLSKYKIKDVELKNRIVMPPMCMYTADEDGMVNHFHQAHYIS
                     RAIGGVGLIIVEATAVVPNGRITNQDLGIWSDDHVPGLRSLVENMHRYGSKVAIQLAH
                     AGRKCESGDEYIVAPSAIRYSDRYRMPKELTLEEIKDLVLSFKEAARRAYEAGFDIIE
                     IHAAHGYLINEFLSPVTNKRTDRYGGSLENRVRFLKEILEEVRKVWPEEKPILLRVSG
                     TEYVEEGIDENEMVKIINMLKDQIDMVHVSSGGLVSVKIPVYPGYQVKLAETIKAECN
                     MPTIAVGLIYKFEQIEEILSNNRADLVAIGRGLLRDPNFVLNMAYENKLDMDYPRQYE
                     RGYR"
     gene            complement(3263..>3624)
                     /locus_tag="VK031_04400"
     CDS             complement(3263..>3624)
                     /locus_tag="VK031_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLS53190.1"
                     /translation="SAKDFVVENINVAGQLIDGKLSKLPDQIKIKPGEGQTFKYKGER
                     IGAFRDDRGKLHFVDTTCTHLGCELNWNSAERSWDCPCHGSRFTYEGKIIQGPAVKDL
                     SFDKDTNILTKLIKEDF"
BASE COUNT         1037 a          786 c          605 g         1196 t
ORIGIN      
        1 tgtatcaaat ctttggctaa tttcaaggtt aataggaatg attctagcta taatggtata
       61 ttttaaatta ggtccatcgt ggatttattc tgaagataca ggtggattaa tcttatatga
      121 tttaatccag ggtctttttg ccatattttt atttgcagga tttttactac cctttttaac
      181 agactttggc ctactggaat ttgtaggtgc cctactaact cctataatga ggcctatctt
      241 tggcctacca ggcagggcat ccatagactg tatagcttct tgggtagggg atggaaccat
      301 aggggtggcc ttaacaaaca agcaatatga ggagggctat tatactaata aggaagcctc
      361 aattattgct acaacttttt cggccgtatc cattaccttt tgcctggtag tattaggcca
      421 ggtggatctg gcccacttat ttggccccta ctacctaaca gtggcagtgg caggcatagt
      481 ggcagccatc atagttccaa agattccgcc cttatctaga aagtccaatg attattataa
      541 aggcataaaa aaggacaaag gtgaaaatgc accagagggc tatacaaata taagttgggg
      601 tttagagttg gccctaaaaa aggctgatga aagttttaag cctagagaat ttatagctaa
      661 tggtacaaaa tctgtattag atctttggct aggtgtatta ccgattatca tggcctttgg
      721 taccatagct cttataattg cagaatttac acctgttttt caatggttag gtatgccctt
      781 tattcctata ttaaaactat taagggtccc ctatgcagtg gaggcctccc aaacaattat
      841 ggtcggcttt gcagatatgt tcctaccgtc tgttataggg gcaagtattc ccagtgaaat
      901 gaccaggttt attatagcaa gcctatctgt aactcagtta atttatttat ctgaggcagg
      961 ggctgtaatg ctaggagcaa aaatggatat tagtttaaag gatttattta ttatctttat
     1021 agaaagaacc ttaattacct tgccaattat agtattggca gccaatataa tattttaaat
     1081 aaaaaaccca ggcttaagcc tgggttttat taatgatata ctgccatata gggttttgga
     1141 tagtctatgt tcaacttatt ttcatgggct atatttaaga caaagtaagg ctcatttaaa
     1201 agttttctgc caagggccac taaatctgct cgaccattgg ataagatttc ctcaatctgt
     1261 tcaaagtttc taatttgtcc agcagtaatc gtaggaatat tacatttatc tttaataatt
     1321 tctgcataag gcgcctgata acctggatag gacttgatct tgacatttac caggccgccg
     1381 gtgcttacat ccaccacatc tatataatct ttaacctgat taattatttt taccatctct
     1441 ttttcatcca taccaccatc tacataatcg gtggcagaca ctctaataaa tataggctta
     1501 tctttaggcc agaccttact tatttcctca agtatttcct ttaaaaacct gacccggcct
     1561 tccaaacttc ccccatattc atctgtcctt ttattggtaa taggggaaag aaattgatgg
     1621 atcaaataac catgggcagc atggatttca actatatcaa agcctgcctc attagctctt
     1681 ctggcagctt ctttaaagga cttaaccata ttccttatat catcctggga catttctttg
     1741 ggtttttcat atttgtcact gtgtcttagg acagatgggg ccacaaggct ttcgcctgca
     1801 actgagcact tccttccagc atgggacagt tggatggctg ttttactgcc gtagcttttt
     1861 accttgtcca ctaggtcctt aaggccagct atatgatcat cactccaaat ccctaaatcc
     1921 ttattagata tcctgccatt aggttctatg gcaatagatt cactaataat aagacctaca
     1981 ccacctactg ccctggtggc atagtggagt ttatgaaaat ccttaaccat acccttatca
     2041 tcacttactc ccatgcacat gggaggcatg acgattctat ttttaagttc tatgtcctta
     2101 atcttgaact tggttgcaaa tttcaaatta tcaccatcct tctataaaga ggtttacatg
     2161 gaaaaatata tcttaagtct actaacgata gcctcgctca tattgtctag gataatccat
     2221 atctagctta ttttcatagg ccatatttaa aacaaaatta ggatccctta aaagccctct
     2281 acctatggcc actagatctg ccctattatt agataaaatt tcttctatct gctcaaactt
     2341 atatataaga cctacagcaa tagtaggcat attacattca gccttaattg tttctgccaa
     2401 ttttacctgg taacctggat aaactggaat cttcacactg actaatccac cactacttac
     2461 atgtaccata tcaatttggt ccttaagcat gttgataatt ttaaccattt cattttcgtc
     2521 aatgccctct tccacatatt ctgtaccaga tacccgaagg agtataggct tttcttcagg
     2581 ccaaaccttt cttacctctt caaggatttc tttcaaaaat ctaaccctgt tttccaagct
     2641 acctccatat ctatctgtcc ttttattggt aacaggagac agaaactcat taatcaaata
     2701 accatgagca gcatggattt caattatatc aaagcctgcc tcataagccc ttctggcagc
     2761 ttctttaaag gacaagacca agtctttgat ctcctccagg gtcaactcct taggcatcct
     2821 atacctatcg ctatatctaa tggctgaagg agccactata tattcatcac cggattcaca
     2881 tttccttcca gcatgggcca attggatggc caccttgcta ccgtaccggt gcatattttc
     2941 caccaaggat cttaaaccag gcacatggtc atcactccat attcctagat cctggttggt
     3001 aattctgccg ttaggcacca ctgctgtagc ttccacaata attaaaccta cgccacctat
     3061 ggccctagat atataatggg cctgatggaa atgattgacc ataccatcct catcagctgt
     3121 atacatacac atgggaggca tgactattct atttttaagc tctacatcct taatcttata
     3181 tttgcttaat gttttcatta aatcaccacc ctaaaaatta tataccctaa tctaataaat
     3241 aaaacaccag gctttttgcc ttttaaaaat cctctttaat taatttagtc aatatatttg
     3301 tgtctttatc aaagcttaaa tctttcactg caggaccttg gattatttta ccttcataag
     3361 taaatctaga accatgacaa gggcagtccc aagacctttc tgccgaattc cagtttaact
     3421 cacagcctag atgggtgcag gtggtatcta caaaatgaag cttacccctg tcatctctga
     3481 aggcaccgat tctttctcct ttgtacttaa aagtttgacc ttcccctggc ttgattttta
     3541 tttgatcagg cagtttggat aacttgccat ctattaattg gcctgccaca tttatatttt
     3601 caactacaaa atccttggct gaag
//
LOCUS       DATGSI010000289         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_301479, whole genome shotgun sequence.
ACCESSION   DATGSI010000289 DATGSI010000000
VERSION     DATGSI010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301479"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VK031_04405"
     CDS             <1..972
                     /locus_tag="VK031_04405"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HLS53191.1"
                     /translation="QSSNDTFPTAMSISAIMEIEKDLLPAIEKLENTFKRLEKENEGI
                     IKTGRTHLQDAVPIAFSQEISGWTEMLKKGKRHILDSMESLRELALGGTAVGTGLNAH
                     KDFGKIVAEEIEKLTGTRFVTAENKFYSLTGKSDLANAHGALKALAADLMKIANDIRW
                     LASGPRCGIGEIFIPENEPGSSIMPGKINPTQAEALTMVTAQVMGNDVAIGVGASQGN
                     FQLNVYMPMMIYNFLQSVRLLSDGINSFNTHCAEGIKPNKDKMKENLDKSLMLVTALN
                     PHIGYEKSAKIANLAHDEGLSLKEAAVKLGYLTETEFDNLVKPEEMV"
     gene            1134..>1516
                     /locus_tag="VK031_04410"
     CDS             1134..>1516
                     /locus_tag="VK031_04410"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HLS53192.1"
                     /translation="MTLLSPSILSADFSDLKGEIQKVEEGGADYIHLDVMDGHYVPNI
                     TFGAPVIKKLRKVTDLPFDVHLMIDRPERYIEDFVDAGADIITVHQESTTHLHRAIQL
                     IKNLGVKAGVSLNPSTPLDSIEYIL"
BASE COUNT          551 a          233 c          340 g          392 t
ORIGIN      
        1 caaagttcta acgatacttt cccaacggcc atgagtatat ctgccataat ggaaatagag
       61 aaggacctat tacctgccat cgaaaaattg gaaaatacat ttaaaaggtt ggagaaggaa
      121 aatgaaggaa ttataaaaac aggcaggacc catctgcaag atgcggtacc aatagctttt
      181 tcccaggaaa taagtggttg gacagagatg cttaagaagg gaaaaagaca tattctagat
      241 tcaatggaat ctttaaggga attggcccta ggaggtacag cagtaggtac tggcttaaat
      301 gcacacaagg attttggaaa gattgtggca gaagaaattg aaaaattaac tggaaccagg
      361 tttgtgacag cagagaataa gttctattcg ctaacaggta aaagtgattt ggccaatgcc
      421 cacggggcct tgaaggcttt ggctgcagac ttaatgaaga tagccaatga tattaggtgg
      481 ttggccagtg gacctagatg cggtataggt gaaatattta taccagaaaa tgaaccaggt
      541 agctccataa tgcctggaaa aatcaatcct actcaggcag aagctctaac catggtaaca
      601 gcccaagtaa tggggaatga tgtggccata ggtgtaggag catcccaggg gaatttccag
      661 ttaaatgttt atatgccaat gatgatttac aacttcctac aatcagtaag attgttaagt
      721 gatgggataa attcctttaa cacccactgt gcggaaggaa ttaagcctaa caaggataag
      781 atgaaggaaa acttagataa atctctcatg ctagtaactg cattaaatcc acatatagga
      841 tatgaaaaat cggctaagat agctaatttg gctcacgatg aaggcctatc attaaaagag
      901 gcagcggtta aactaggcta tttaaccgag acagaattcg ataacctggt taaaccagag
      961 gaaatggttt aagagaaaac tagaatccta tttagggttc tagtttttta tcttataaaa
     1021 taaggcatta ggataataat taagagtaaa tattaaaaaa gaagagaaac tatgatatca
     1081 tagtgagtat ataagtaata gaggtaagta aagaattact aaggaggata aacatgacat
     1141 tactttcgcc ttcaatatta tcagcagatt ttagtgactt aaaaggagaa atacagaaag
     1201 tggaagaggg aggggcagac tatatacatc tggatgtaat ggatggccat tatgtgccaa
     1261 atattacctt tggggcacct gtaataaaaa aattgagaaa ggttactgac ctaccctttg
     1321 atgtgcattt gatgatcgat aggcctgaaa gatatataga agattttgta gatgcagggg
     1381 cagatattat tactgtccac caggaatcaa ccacccattt gcatagggcc atccagctga
     1441 taaaaaattt aggtgtaaag gcaggggttt cacttaatcc atctacacct ttagacagta
     1501 tagaatatat attaga
//
LOCUS       DATGSI010000290         1183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_493321, whole genome shotgun sequence.
ACCESSION   DATGSI010000290 DATGSI010000000
VERSION     DATGSI010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493321"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1183
                     /locus_tag="VK031_04415"
     CDS             <1..>1183
                     /locus_tag="VK031_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HLS53193.1"
                     /translation="HIEEYESEARDTKLGPEEITRDIPNVGEDMLRDLDERGIVRIGA
                     EVKAGDILVGKVTPKGETELTAEERLLRAIFGEKAREVRDTSLRVPHGESGIVVDVKI
                     YSREDGDELLPGVNQSVKVYVATKRKISVGDKMCGRHGNKGVISRILPEEDMPYLPDG
                     TPIEIVLNPLGVPSRMNLGQVLEVHLGLAAKKLGWHIATPVFDGANEYDIMEALKKAD
                     YPEDGKIMLRDGRTGEYFNSPVTVGYMYMLKLHHLVDDKIHARSTGPYSLVTQQPLGG
                     KAQFGGQRFGEMEVWALEAYGASHTLQELLTVKSDDIVGRVKTYEAIVKGENIPEPGV
                     PESFKVLIKELQSLALDVKVLSEDDEIEIKESDDDLTDLDMMGEELSAEDKDQDDDKP
                     KA"
BASE COUNT          418 a          178 c          301 g          286 t
ORIGIN      
        1 ttcatattga agaatatgaa tctgaagcaa gagatacaaa actaggtcca gaagaaatta
       61 ctagagatat acctaatgtg ggagaagata tgttaagaga cctggacgaa agaggaattg
      121 tcagaattgg ggcagaggta aaggcaggag atatattagt aggtaaggta acccctaaag
      181 gagaaacaga attaacagca gaagaaagac tattaagggc catttttggg gaaaaggcta
      241 gggaagtcag ggacacctcc ttaagagttc cccacggaga aagtggaatt gtagtagatg
      301 tgaaaattta ttccagagaa gatggggatg aactattgcc aggtgttaat caatctgtca
      361 aggtatatgt ggccaccaag cgtaagataa gcgttggaga taaaatgtgt ggtcgtcatg
      421 gtaataaggg cgtaatttcc agaatcttac cagaagaaga catgccttat ctaccagatg
      481 gtacgcctat agaaatagtc ttaaatccat tgggagtacc ttcaaggatg aacctagggc
      541 aggtgctaga agttcaccta ggcctggcag cgaaaaaatt aggttggcat atagctacac
      601 cagtctttga tggtgccaac gaatacgaca ttatggaggc cctaaagaag gctgattatc
      661 cagaggacgg aaagataatg ttaagggatg gaagaactgg agaatatttt aatagtccag
      721 ttactgtagg ctatatgtat atgctcaagc tccatcactt agtagacgat aagatacatg
      781 ccagatctac aggaccatat tctttggtta cccaacaacc attaggcggt aaggcacagt
      841 ttggaggcca aagatttgga gaaatggagg tttgggcctt agaggcatat ggtgcttcac
      901 atactttaca agaattgtta acagttaaat ctgatgatat agtaggtaga gttaaaactt
      961 atgaagctat tgtaaagggc gagaatatac ctgaaccagg agtaccagaa tccttcaagg
     1021 tattaatcaa agaattacaa agcttggcat tagatgtaaa agtcttaagc gaagatgacg
     1081 agatagaaat taaagaatca gacgatgatt tgacagacct tgatatgatg ggcgaagaat
     1141 tgtcagccga ggataaggat caggatgatg ataagcctaa ggc
//
LOCUS       DATGSI010000291         4432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_210910, whole genome shotgun sequence.
ACCESSION   DATGSI010000291 DATGSI010000000
VERSION     DATGSI010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210910"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..876
                     /gene="sigF"
                     /locus_tag="VK031_04420"
     CDS             118..876
                     /gene="sigF"
                     /locus_tag="VK031_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HLS53194.1"
                     /translation="MTRGKRKRELLSHEETMELIKKAQKGDQKSREKLVESNIGLVRS
                     VLKGFVNRGHDVEDLFQIGSIGLLKAIDKFDHSFKVKFSTYAVPMIMGEIKRFLRDDG
                     LIKVSRSLKQVAGQVKRAGERLFKENGREPTIEEIAEEIGIDSEEIVMAIESSYQPEY
                     LHSVIHHNDGSPLYLIDKVGMDTFADEDMIDSIILKDILSQLKPRDRQVIILRYFKDQ
                     TQTEVAKKLGISQVQVSRIEKRIIGEIRNLMLKA"
     gene            943..1104
                     /locus_tag="VK031_04425"
     CDS             943..1104
                     /locus_tag="VK031_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53195.1"
                     /translation="MRLINRKSFLITIVFLIISFALLLYKYLYVFTKDAEPPKRATYV
                     KVEIEGSCI"
     gene            1101..1727
                     /locus_tag="VK031_04430"
     CDS             1101..1727
                     /locus_tag="VK031_04430"
                     /inference="COORDINATES: protein motif:HMM:NF023586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AA"
                     /protein_id="HLS53196.1"
                     /translation="MSKETVYIALRDRMIINTNVLLTIKDLGQVFCNNKAIEDYIGDI
                     EIYKSPAEENWDYIDADYIINKAVGNNPSVDINMIGATEVLLEIKSREKERKIFQFIK
                     VTFILIVLFFGSGIAIINFHEDVNMTQSLEKIYYSFTGVKKTRPLIMTIPYSIGLGVG
                     IIGFFNRVVSSSHRRKKEPGPMELESYLYDRDMDQYILDDLKKKDDKT"
     gene            1745..2155
                     /locus_tag="VK031_04435"
     CDS             1745..2155
                     /locus_tag="VK031_04435"
                     /inference="COORDINATES: protein motif:HMM:NF025155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AB"
                     /protein_id="HLS53197.1"
                     /translation="MFIKLVVVLIALGGGLVVGGALAAFLTLIKLVPRLVQLTETRKS
                     IKLYEYSLSIGSLIFIIFYFSELSLKFSNILTSIFSLMMGIFVGLFSSALAEVLNVIP
                     VLTKKLKVEDEKQYIIYTLTLGKVAGSLYFWLYY"
     gene            2172..2615
                     /gene="spoVAC"
                     /locus_tag="VK031_04440"
     CDS             2172..2615
                     /gene="spoVAC"
                     /locus_tag="VK031_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HLS53198.1"
                     /translation="MEKLNKQEYQKYIKDKVPKPTYLRNLIFAFLVGGLICTFGEFIN
                     NYLANLGLNEESIATGTSIAMVFLGSLLTGLGIYDRIGKIGGAGASIPITGFANSIVA
                     PAMEFKREGFIFGVAAKMFIIAGPVLVYGIGGSVLVGILKILLKR"
     gene            2625..3644
                     /gene="spoVAD"
                     /locus_tag="VK031_04445"
     CDS             2625..3644
                     /gene="spoVAD"
                     /locus_tag="VK031_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HLS53199.1"
                     /translation="MTSKRIGNQTVKLSNPPSIVGYYSIVGPKEGDGPLKKYFDEILE
                     DDLWGQDSWEKAESKIQEATIRAAIEKSESTLNDIDILISGDLLNQIVASGYSARQLA
                     LPYFGLYGACSTMTESLSLGAMLIDGGFANKIVCATSSHFSSAERQFRFPLEYAAQRV
                     FSSQWTVTGSGATVLSNQGTGPYIKYVTPGRIIDPGVTDVSNMGAAMAPAAMDTLLHH
                     FWDTGFGPEDYDIIITGDLGRVGKAIVLDVMKKEGFDLSKVYTDCGVEIFDHEKQDTH
                     AGGSGCACSAVVFNGYILKEMKKGKYKRVLFAATGALHSTTITQQGESIPSICHAVTV
                     DISKE"
     gene            3656..4012
                     /gene="spoVAE"
                     /locus_tag="VK031_04450"
     CDS             3656..4012
                     /gene="spoVAE"
                     /locus_tag="VK031_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HLS53200.1"
                     /translation="MEYFKAFFVGGTICLIGQIILDRTKLTSAHILVTFLMAGVVLGG
                     LGLYEPIVKFAGAGATVPISGFGYALSKGAIKGAKTKGILGAFTGGLEATAGGIAAAV
                     LFGYIMAILFNPKTKD"
     gene            complement(4038..>4432)
                     /locus_tag="VK031_04455"
     CDS             complement(4038..>4432)
                     /locus_tag="VK031_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HLS53201.1"
                     /translation="VHVEVAICKVTGKVATEFCPEDEVEMKVFVKRTPPYDPSENNNI
                     KPDDYGYQVPTKECDIHDESSLPPEEDDSKDEDEQNGNGGENGENGNGEGQDSNDKNG
                     DNGDKNNQDNNGQTNGNGPVEGEPGNKD"
BASE COUNT         1431 a          648 c          957 g         1396 t
ORIGIN      
        1 ctttacagtg atggaaactt ttatggacag cctacaggtg gaaagcaatg agggcaaggg
       61 gacaattgta aaaatgacca aaaaaattga aaatctctct gataaggaga ggtaaagatg
      121 acaaggggta aaagaaaaag ggagctgtta tctcatgaag agaccatgga acttattaaa
      181 aaggctcaaa aaggagatca aaagtctaga gagaaattag tagagtctaa tataggttta
      241 gtaaggtcag tcttaaaggg ctttgtaaac aggggtcatg atgtggaaga cttatttcaa
      301 ataggcagta ttggtttatt aaaggccata gataaatttg atcattcctt taaggtgaag
      361 ttttccacct atgctgtacc tatgattatg ggggaaatta aaaggtttct tagggatgat
      421 ggtttaatta aggttagtag atcattaaaa caagttgccg gacaggtaaa aagggccggg
      481 gaaaggctgt ttaaggaaaa tggcagggaa cctaccatag aggaaatagc agaggagata
      541 ggcatagata gcgaagaaat agtaatggct atagaatcca gctatcaacc agagtatctt
      601 cactcagtaa tccatcataa tgatgggtca ccgctttatc taatagacaa ggtaggtatg
      661 gatacctttg cagatgaaga tatgatagat agtattattt taaaagatat tttaagccag
      721 ttaaaaccta gggacaggca ggtgattata cttaggtatt ttaaggatca aacgcaaaca
      781 gaagtagcta aaaaactagg aatatcacag gttcaggtat ccagaatcga aaagagaata
      841 atcggagaaa ttagaaatct catgttaaag gcataatata tgcctttttt ttgtattaaa
      901 ttttctatcc tgtaaataat aaagttagaa tggagggata atttgaggct aataaatcgt
      961 aaaagttttc ttataactat tgtgttttta ataatatctt ttgctttatt attatataag
     1021 tacctttatg tttttactaa ggatgcagaa ccacctaaaa gggctaccta tgtaaaagta
     1081 gagatagaag gtagttgcat atgagtaagg aaactgttta tatagcctta agggatagga
     1141 tgattattaa tacaaatgtg cttttgacta taaaagactt aggacaggta ttttgcaata
     1201 ataaggcaat tgaagattat ataggtgata ttgaaattta taagtcgcca gctgaagaaa
     1261 attgggatta tatagatgct gattatatta taaacaaagc agtaggcaat aatccctctg
     1321 ttgatataaa tatgataggg gcaacggaag ttttgcttga gattaaatct agagaaaagg
     1381 agagaaaaat ttttcaattt attaaggtaa cctttatact gattgttttg ttttttgggt
     1441 ctggtattgc aataatcaat ttccacgagg atgtgaatat gactcagtct ttagagaaaa
     1501 tatattatag ctttacagga gtgaaaaaaa caagaccatt aattatgacc atcccctatt
     1561 ccatagggct aggtgtaggg attattggtt tcttcaatag ggtggttagc tccagccata
     1621 ggagaaagaa ggagcccggt ccgatggagc tagaatcata tttatacgat agggatatgg
     1681 atcaatatat actagatgat ttaaaaaaga aagatgataa aacctagcaa ttaggggtga
     1741 gtatatgttc atcaagttgg tagtagtgct tattgccctt ggaggtgggt tggtagtggg
     1801 aggggccttg gcggcctttt taaccctaat aaaattggtt ccaagattgg tacagcttac
     1861 ggaaactagg aaaagtataa agctatatga gtatagttta tctattggca gtttaatttt
     1921 tataattttt tattttagtg aattaagtct taagttttct aatattctta cctctatatt
     1981 tagcctaatg atgggaatct ttgttggact tttttcatct gctttggctg aggtcttaaa
     2041 cgttattcca gttttaacta agaagttaaa agtagaggat gaaaaacagt atataattta
     2101 tactttaact ctagggaaag tagctggatc cctatatttt tggttgtatt actaaaactg
     2161 ggaggtttaa tatggaaaag ctaaataaac aggaatacca aaaatatata aaggataagg
     2221 taccaaagcc tacctattta agaaacttaa tattcgcatt tctagtaggt ggattaattt
     2281 gtacctttgg tgaatttatt aataattatt tagccaattt aggcttaaat gaagagtcaa
     2341 ttgctactgg aacttcaata gccatggtgt ttcttggttc actattaaca ggattaggca
     2401 tatatgacag gataggtaaa attggagggg caggagcctc catacctata acaggttttg
     2461 ccaactcaat agtagctcct gccatggaat ttaaaaggga aggctttatc tttggagtgg
     2521 cagctaaaat gtttattata gctggacctg ttttagtcta cggtataggt ggttcagttc
     2581 tagttgggat tttaaaaata ttgctaaaaa ggtagggaaa aataatgact agcaaaagaa
     2641 taggaaatca gactgtaaag ctatcaaatc ctccctccat agttgggtat tatagtattg
     2701 ttgggccaaa ggagggggat ggtcctttaa aaaaatattt tgatgaaatt ttagaagatg
     2761 atctatgggg ccaggactct tgggaaaagg ctgaaagtaa aatacaggag gctaccataa
     2821 gggctgccat agaaaaaagt gaatctactt taaatgatat agatatttta atttctggtg
     2881 acttgttaaa ccaaattgtg gcctctggat attctgcaag acaattggcc ttgccttact
     2941 ttggcttata tggagcatgt tctaccatga cagaatcctt atctttagga gccatgttaa
     3001 tagatggagg ttttgccaat aaaattgtat gtgcaacttc cagccatttt agctctgctg
     3061 aaagacaatt tagatttcca ttggaatatg cagcccaaag ggttttttct agtcaatgga
     3121 cagttacagg ctctggggct actgtacttt caaatcaggg cacaggtcca tatataaaat
     3181 atgtgactcc aggcaggatt attgaccctg gggtaactga tgtatcaaat atgggagctg
     3241 ccatggcacc agctgccatg gacacacttt tacatcattt ttgggatact gggtttggac
     3301 cagaggatta tgacataatt attacaggtg atttaggacg ggtggggaag gctatagtcc
     3361 tagatgtaat gaagaaggaa ggttttgatt tatccaaggt atatacagat tgtggagttg
     3421 agatatttga tcatgaaaaa caggatactc acgctggtgg cagtggttgt gcctgctctg
     3481 ctgttgtttt taatggttat atattgaaag aaatgaaaaa aggaaaatat aagagggtcc
     3541 tatttgccgc cacaggagcc ttgcattcta ctaccataac ccagcaaggt gaatctatac
     3601 cgtctatttg ccatgcagta actgtagata ttagtaaaga atagggatga taaatatgga
     3661 atatttcaaa gcattctttg taggaggaac aatctgccta atagggcaga taatactgga
     3721 tagaactaaa ctaacctcgg cccacatatt ggttaccttt ttaatggcag gagttgtttt
     3781 aggtgggcta ggattatatg aacctatagt taagtttgct ggagcaggtg ctacagttcc
     3841 catctctggg tttggatatg ccctatctaa gggggccata aagggagcta aaacaaaagg
     3901 aatccttgga gcctttacag gtggactgga ggctacagct ggaggtatag ctgctgctgt
     3961 tttgtttgga tatataatgg caattttatt taacccaaaa acaaaggact agattttcat
     4021 ctagtccttt ctttatatca atccttattc ccaggttcgc cctctacggg gccattacca
     4081 tttgtttgac cattgttgtc ttgattattt ttgtctccat tgtctccatt tttatcattg
     4141 gaatcttgcc cttcaccatt accattttcg ccattttctc caccatttcc attttgctca
     4201 tcttcatcct ttgaatcatc ttcttctggt ggaaggctac tttcatcatg gatatcacat
     4261 tcttttgtag gaacttgata gccatagtca tcaggcttaa tattattatt ctctgatgga
     4321 tcatatggtg gcgtacgttt aacaaatact ttcatttcca cctcatcttc aggacaaaat
     4381 tctgtagcta cctttccagt taccttacat atggcaacct ccacgtgcac at
//
LOCUS       DATGSI010000292         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_66888, whole genome shotgun sequence.
ACCESSION   DATGSI010000292 DATGSI010000000
VERSION     DATGSI010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66888"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1374
                     /locus_tag="VK031_04460"
     CDS             160..1374
                     /locus_tag="VK031_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HLS53202.1"
                     /translation="METAEGNTIEVILNRPHEGQQTFLDSPKRFKVLMCGRRWGKSDI
                     SKNLSIDYMLNSKSVAYITPSYQLAKVFYEDVLLILPNELIKSKNKSDLVIELITGGS
                     LSFFTGEKPDALRGRKFHLVIIDEASYVKDLKNAWNNAIRPTLTDYRGDAIFISTPRG
                     KDFFNSLFDRGQNKEEKEWESFRFTSYENPYIDPAEIEMAKMELPENAFRQEYLAIPS
                     ENVANPFGMDNIQKNVIKKLSDKPSVIYGIDLAKTTDWSVIIGLDGDGNMSHYDRFQL
                     DWTATKDKIRALPRSVVKHIDSTGVGDVIVEELQKEMSNIEGFKFTGSSKPQLILSLI
                     KSIEEGKVKYTEDLVDELSVFEYKLGVSGHVKYEAMSGYHDDKVIALALANYLWKAKQ
                     QTGNWSIVVRRK"
     gene            1400..1990
                     /locus_tag="VK031_04465"
     CDS             1400..1990
                     /locus_tag="VK031_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53203.1"
                     /translation="MNKNSRKTTVTVEGFEIPTSWKDITYNQYMRMVKLELLPETDGV
                     SKINNSISIVALILGISKKEIENLKASSFMEIQQHLEFLSTDIPKTRKTKWKFTPVRK
                     LTMDKWLAYEKYSVDVEENLLKILSLMQEEYTEEEIGNMEVGEVLHGFFTLERNSNLS
                     IRRSLLSLNLKMIKWALNQKIQNLKKRLRIRRRGQR"
     gene            2008..2193
                     /locus_tag="VK031_04470"
     CDS             2008..2193
                     /locus_tag="VK031_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53204.1"
                     /translation="MNNEFQSKWGWLDLCRKVAEYLHTDFFNILENRSVVEVFTITTM
                     MMDDLDRKSIKSTKVGY"
BASE COUNT          890 a          303 c          522 g          702 t
ORIGIN      
        1 tagaagaatt gttactacag cttaagccta atgaaagagc taagttctta acagacttat
       61 tgaagtatgc tttacctgct ttaagttcag tagatatgac agctactgta gataatagaa
      121 taaccagtat tgaagtaaag tataatacta aagagaataa tggagacagc agagggtaat
      181 acaatagaag taatacttaa tagacctcac gaaggacagc agacattctt agatagtcct
      241 aagaggttta aggtacttat gtgtggtagg agatggggta agtcagatat atctaaaaac
      301 ctatctatag actatatgct taactctaag agtgtagctt atataactcc ttcctatcag
      361 ttagctaagg tattttatga agatgtatta ttaatacttc ctaatgagtt aataaagagt
      421 aagaataaat cagacttagt tatagagctt attactggag gtagtctatc tttctttact
      481 ggagagaagc cagatgcttt aagagggcgt aagtttcatt tagttattat agatgaagct
      541 tcctatgtaa aggatttaaa gaatgcgtgg aataacgcta ttagacctac tcttacagac
      601 tatagaggag atgctatatt cattagtaca cccagaggta aggatttctt caatagtcta
      661 tttgacagag gtcaaaataa ggaagagaag gagtgggaat cttttaggtt cacttcttat
      721 gagaatcctt atatagaccc tgctgagata gagatggcta agatggaact acctgagaat
      781 gcttttagac aggagtattt agctatccct agtgagaacg tagctaaccc ttttggtatg
      841 gataatatac agaagaacgt tattaagaag ctatctgata aaccttctgt tatctatgga
      901 atagacttag ctaagactac tgactggagt gttattatag gcttagatgg agatggtaat
      961 atgagccatt atgacagatt ccaattagat tggactgcca ctaaagataa gataagagca
     1021 ttacctagaa gtgtagttaa gcatatagat agtacaggag tgggagatgt aattgtagaa
     1081 gagttacaga aagaaatgag taacatagaa ggatttaagt ttacaggttc ttctaaaccg
     1141 caacttatcc tatctcttat taaatctata gaagagggaa aggtaaaata tacagaggac
     1201 ttagtagatg aactctctgt atttgaatat aaactaggcg tttcaggaca cgttaagtat
     1261 gaagcaatgt ctgggtatca cgatgacaaa gtaatcgctc tggcgttagc taattacctc
     1321 tggaaggcta aacagcagac gggtaattgg agtatagtag taagaagaaa ataatacaac
     1381 attaataatg atgacaaaga tgaataagaa cagtagaaaa acaacagtaa cagtagaagg
     1441 gtttgagatt cctaccagtt ggaaggatat tacttataac cagtatatga gaatggttaa
     1501 gttagaatta cttccagaga cagatggagt atctaagatt aataacagca tcagcatagt
     1561 agctttgata ttaggtataa gtaagaaaga gatagagaat cttaaagcta gtagttttat
     1621 ggagatacaa cagcatttag agttcttatc tacggacata cctaagacac gtaaaactaa
     1681 gtggaagttt actcctgtta gaaagttgac tatggataag tggttagctt atgagaagta
     1741 ttcagtagat gtagaggaga atcttttaaa gatattatcc cttatgcagg aagaatatac
     1801 ggaagaggaa ataggtaata tggaagtcgg ggaggtgctt cacggttttt ttacgttaga
     1861 aaggaactcc aatctctcta taaggcgttc cctgctatct ttaaatctaa agatgataaa
     1921 gtgggcgttg aatcagaaga tacagaatct aaagaagaga ctgagaataa ggagaagagg
     1981 tcagcgttag aagaagagca ggattttatg aataatgagt ttcaatctaa atggggttgg
     2041 ttagacttat gtaggaaagt agctgaatac ttacatacgg acttctttaa catattagaa
     2101 aatagaagcg tagtagaggt gtttaccata actactatga tgatggatga cttagataga
     2161 aagagtatta aaagtactaa agtaggttat taaatatata tttgtagtaa tttctatgag
     2221 taagccctat atagatacaa aacatactct caaaaaatac tacatttaaa aatagaacaa
     2281 aatggcatta cacgatttag ctaaattaga agctttaaat gatattggag aagatggaag
     2341 ttcttttgat agagacttag taggagcaga gaaagcctgt ggagactttg ttaaaagagt
     2401 tgctactaat ataatag
//
LOCUS       DATGSI010000293         1307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_116893, whole genome shotgun sequence.
ACCESSION   DATGSI010000293 DATGSI010000000
VERSION     DATGSI010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116893"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..>1307)
                     /gene="phnE"
                     /locus_tag="VK031_04475"
     CDS             complement(91..>1307)
                     /gene="phnE"
                     /locus_tag="VK031_04475"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HLS53205.1"
                     /translation="IWAALLVSVFSIGKFSGIVALTIIAFLMSLKLFREYIEAINENQ
                     LNSIRSVGASSLQVLRYCVLPHILDLSISVFFLTFETNIRSATILGLVGAGGIGQIMW
                     RDLNHLRYDNLSTLILILFFTILSIDFLSLYIRRLLNKTTIKFRTIKNYQRFKLAKIV
                     YTPLLIIGVLGFIAQSFNIGYDRFLLGLNQGATIIFRMIRIDISYWPNLVKGIKESFF
                     IAVFATISGALASLFLSYFVAYNTSPRSWLSLLSKGIINILRTFPPIITAIIFFRGVG
                     PGPLAGAMALSVYTTGVLTKMYSELLESTEENIQNSIISTGASKFQSYRHGLIPHTFP
                     TFISLTLYRLESNIRSSTILGLIGAGGIGSILSMNINWRNWEKVGLLLLGISLMVILI
                     DILSNYLRKLIS"
BASE COUNT          440 a          238 c          212 g          417 t
ORIGIN      
        1 agcaaatacc ggctttaaaa taatcataac ttcctcccat aacttttata gctagtattt
       61 gcattcttcc atatatttaa cctaaagtat ttatgagatt agttttctca aataattact
      121 taaaatatct atcagtatta ccattaagga tatgcctaaa agaagtaatc ccaccttttc
      181 ccaatttctc cagtttatat tcattgaaag aatggaacct attcctccag ctccaattag
      241 acctaaaatt gtggagcttc ttatattgga ttccagccta tacaaggtta gacttataaa
      301 ggttggaaaa gtatggggga taagcccatg tctataactt tgaaattttg aggcccctgt
      361 agatataatg ctattttgta tattctcctc agtactttct aatagttcac tatacatctt
      421 ggttaaaaca cctgttgtat aaacacttag ggccatggct ccagccaagg ggccaggtcc
      481 tacgcctcta aagaagataa tggctgttat aataggggga aaggtcctta gtatatttat
      541 tattccctta gatagcaggc tgagccagga tctaggtgat gtattatagg ccacaaaata
      601 agataaaaac aaggaggcta gtgcccctga aatagtggca aatactgcaa taaaaaagct
      661 ttcttttata cctttgacca ggttgggcca ataggatata tctatcctta tcattctgaa
      721 tataattgtt gctccttggt ttaagcctag taaaaatcta tcataaccaa tattaaaaga
      781 ttgggctata aatcctaaga caccgattat taaaaggggt gtgtagacta tttttgccag
      841 cttaaatctt tgataatttt taattgttct aaatttaatt gtagttttat ttaacagcct
      901 tctaatatag aggcttagaa agtctataga aagaattgta aagaataaga tcaaaatcaa
      961 ggtagacaaa ttatcatacc ttaaatggtt taaatcccgc cacataatct ggcctatacc
     1021 tccagccccc accaagccta atatggtggc acttcttatg tttgtttcaa aggttaagaa
     1081 aaagactgat atggataagt ccaatatgtg gggcaacaca caatacctta aaacctgtag
     1141 agagcttgcc cctacagatc ggatagaatt aagctggttt tcatttatgg cctcaatata
     1201 ttctctaaaa agttttaaac tcatcaagaa agctataatt gttagggcaa ctattcctga
     1261 aaatttccct atactaaata cactaactaa aagggcagcc catatta
//
LOCUS       DATGSI010000294         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_681788, whole genome shotgun sequence.
ACCESSION   DATGSI010000294 DATGSI010000000
VERSION     DATGSI010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681788"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /gene="tsf"
                     /locus_tag="VK031_04480"
     CDS             <1..635
                     /gene="tsf"
                     /locus_tag="VK031_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HLS53206.1"
                     /translation="SLVKELREKTGAGMLDCRNALVETEGDIEKAVDLLREKGLASAS
                     KKAGRIASEGLVDSYIHGGRIGVLVEVNSETDFVAKTDEFKEFVKDIAMQVAAANPKY
                     VSREDVPEEEVEREKSILVQQAINEGKPEHIVEKIVEGRMEKYFEEICLLDQAFIKDP
                     DVKINDLLKEKIAKIGENIIIRRFSRFEVGEGLEKREEDFAEEVAKQMGN"
     gene            700..1413
                     /gene="pyrH"
                     /locus_tag="VK031_04485"
     CDS             700..1413
                     /gene="pyrH"
                     /locus_tag="VK031_04485"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HLS53207.1"
                     /translation="MNPVFKRIVLKLSGEALAGQAGTGIDDDTIKRIATEIKEVHQLG
                     VEISIVVGGGNFWRGKTAKGMDRTTSDHIGMLGTVMNALALQDALENIGVITRVQSAI
                     DMKQVAEPYIRRRAIRHLEKGRVVIFAAGSGNPYFSTDTAAALRAAEVEAEVILVAKK
                     GVDGVYDSDPKFNVDAKKFDTLNYIDILNLGLGIMDSTATSLCMDNKIPLIVFGIDEP
                     NTIFDVVIGKRIGTHVKED"
     gene            1416..1973
                     /gene="frr"
                     /locus_tag="VK031_04490"
     CDS             1416..1973
                     /gene="frr"
                     /locus_tag="VK031_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HLS53208.1"
                     /translation="MYLDVHKQAEARMKKTISVYREELQSIRAGRANPALLDKISVEY
                     YGQMTPLNQVASISAPEPRLLAIQPWDVSLISEIEKQILKSDLGINPSNDGKIIRLQI
                     PRLTEERRVELTRLVRKNAEEAKIAVRNIRRESIDQIRKMEKGKELTEDERIQAENKM
                     QEITDKSIEAIDSITKDKEDELMEI"
     gene            2080..>2506
                     /gene="uppS"
                     /locus_tag="VK031_04495"
     CDS             2080..>2506
                     /gene="uppS"
                     /locus_tag="VK031_04495"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HLS53209.1"
                     /translation="MVVKIQMKKKNPSAEELKKLIDMERLPKHIAIIMDGNGRWAQQR
                     FRPRNFGHQEGMKRVVEIVEVASKLGIKHLTLYAFSTENWKRPKKEIDGLMKILVTYI
                     RRELEKLCRNNVKLQVLGDISKLPTVPREEVERAVKETED"
BASE COUNT          946 a          327 c          588 g          645 t
ORIGIN      
        1 cttcattagt taaggaactt agagaaaaaa ctggagcagg aatgcttgat tgtagaaatg
       61 ccttagtgga aactgaagga gatattgaaa aagctgttga tcttttgagg gaaaaagggt
      121 tagcatctgc ctctaagaaa gctggaagga ttgcttctga aggtttagta gattcatata
      181 tacatggtgg aagaattgga gttttagtag aggttaactc agaaaccgac tttgtggcaa
      241 aaactgatga gtttaaagaa tttgttaaag acattgcaat gcaagtagct gctgcaaatc
      301 ctaaatatgt aagcagggaa gatgtgcctg aagaagaggt agaaagagag aaaagcattc
      361 tggttcagca ggcaattaat gaaggaaaac ctgagcacat tgttgaaaaa atagtagaag
      421 gtagaatgga gaagtatttc gaggaaatct gtttattaga ccaggccttt attaaagatc
      481 ctgatgttaa aattaacgac ttattaaaag agaaaatagc taaaataggt gaaaatataa
      541 tcataagaag attttccaga tttgaagttg gagaaggatt ggaaaaaaga gaagaggatt
      601 ttgctgagga agtagcaaag caaatgggta actagtaaat aggggaacac cgagtgttct
      661 cctattaata atgacttgat acataaggga gtgtattata tgaaccctgt atttaaaagg
      721 atcgtgctaa aacttagtgg ggaagcttta gcaggtcagg caggcactgg aatcgatgac
      781 gatactatta aacgtatagc aacagaaata aaggaagttc accagctagg ggtggaaata
      841 tccattgtag ttggaggagg taacttttgg cgaggtaaaa cagccaaggg tatggataga
      901 accacttcag accatatagg aatgttgggt acagttatga atgccttagc tttacaggat
      961 gccttggaaa acattggcgt cattactcgg gttcaatcag ctatagatat gaagcaggta
     1021 gcagaaccct atataagaag aagggctatt aggcatttgg agaaaggacg agttgtaata
     1081 tttgcggcag gatcaggtaa tccatatttt tcaactgata cagctgctgc cctaagggct
     1141 gcggaagtag aggccgaggt aatattggtt gccaaaaaag gcgtagatgg tgtatatgat
     1201 tcagatccta aatttaatgt agatgcaaag aagtttgata ccttaaatta tattgatatt
     1261 ttaaatttag gattaggaat tatggattct actgccacct cattatgcat ggataacaag
     1321 attcccctaa tagtatttgg gatagatgaa cctaatacta ttttcgatgt ggtaattgga
     1381 aaaaggatag gtacacatgt gaaggaggat taaatatgta tttagatgtt cataaacaag
     1441 cagaagctag aatgaaaaag actatttctg tttacagaga agaacttcaa agtattaggg
     1501 caggcagggc aaaccctgca cttttagaca aaataagtgt tgaatattat gggcaaatga
     1561 cacctttaaa ccaggttgcc agtatatcag ctcctgaacc aagactattg gcaattcagc
     1621 cttgggatgt aagtctaatt tctgaaattg aaaagcagat actaaaatca gatctaggca
     1681 tcaatcctag taatgatggc aaaatcatcc gactgcagat acctagatta acagaagaaa
     1741 gaagagttga gctaacaaga ttagtaagaa aaaatgccga ggaagccaaa attgctgtta
     1801 gaaatattcg tagagaatca attgatcaaa ttaggaaaat ggaaaaaggt aaagaactaa
     1861 ctgaagatga aagaattcag gcagaaaaca agatgcaaga aataacagat aaaagcatag
     1921 aggcaattga ttcaattact aaggataaag aggacgaatt aatggaaatt taatggtttc
     1981 agtggggata tggctaaagt gctttatgaa actagtactt aagataagta aatttaaact
     2041 ggaaatttta aaaccccttc tttcaaagaa ggggtttata tggtggtgaa gatacaaatg
     2101 aaaaagaaaa atccttctgc tgaggagttg aaaaaactaa tagacatgga acgcctaccc
     2161 aaacatatag ctattataat ggatggcaat ggcagatggg cacaacaacg ttttaggccg
     2221 agaaattttg ggcatcaaga aggaatgaaa agggttgttg aaattgttga agttgcaagc
     2281 aaactaggga taaaacattt gaccctttat gccttttcaa cggaaaattg gaaaagacct
     2341 aaaaaagaaa ttgatgggct aatgaaaata ctagttactt atattagaag agaactggaa
     2401 aagctatgca gaaacaatgt aaaacttcag gtcctaggtg atatttccaa gctaccaaca
     2461 gtgccacggg aggaagtgga aagggcagtt aaagaaactg aagata
//
LOCUS       DATGSI010000295         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_252503, whole genome shotgun sequence.
ACCESSION   DATGSI010000295 DATGSI010000000
VERSION     DATGSI010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252503"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1170
                     /locus_tag="VK031_04500"
     CDS             <1..>1170
                     /locus_tag="VK031_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53210.1"
                     /translation="MVVPEDETAYDTGQEITSNNALEITTNLFYELNGFLGDKKTVKS
                     LRYIAKSNEGSWVIIKEIDIDDIKDLPSSIDIENEIEESFNPSTYDYKFIFRNTGLYP
                     FEDEVEAENLYDAIPNRAEAVEEVNGTVLAIGSPEEGYDRPDIETTHAVTYYDPELGS
                     GSSGGGDNFKVSRIRWESHQIIVRRLYVYFSGDPSEGDKVIMRFFPRGNASAVQEEDY
                     TVTISDEIAGLTTVLNNTVNKFNSLGFSGVSFVRQDNYILVVISTNTHSHGIQTIFVD
                     NAGTSTNITTKSVNSLKNNSSYQYSINYYDEYGRPFPIVTGDKFVFETDPLSNTAGLL
                     PQASITIESDPPEGASYYRIGLTENKTTDKYITLVGKVASTTNDDYIIIDLKSLE"
BASE COUNT          442 a          182 c          236 g          310 t
ORIGIN      
        1 catggtagtt ccagaggacg agacagctta cgacacagga caggaaataa catctaataa
       61 cgcattagag ataactacta atctatttta cgaactaaac ggatttttgg gagacaagaa
      121 aacggtaaag agtttaagat atatagctaa atcaaatgaa ggaagctggg tcatcataaa
      181 agaaatagat atagatgaca ttaaagattt accaagcagt atagatatag agaatgaaat
      241 agaagagtct tttaacccat caacttacga ttataaattt atattcagga acacaggtct
      301 ataccccttt gaagatgaag ttgaagctga aaacttatac gatgctatac ctaacagagc
      361 agaagctgta gaagaggtaa atggaacagt attagctata ggaagtccag aagaaggtta
      421 tgacagacca gatatagaaa ctacccatgc agttacttat tatgatccag agttaggtag
      481 cgggtcttct gggggaggag ataattttaa ggtaagtagg atacggtggg agtcgcatca
      541 aattatagtt aggaggttat atgtatattt ctcgggagac ccctctgaag gagataaagt
      601 tattatgaga ttctttccga gagggaatgc tagcgctgta caggaggaag actatactgt
      661 tactataagt gatgagatag ctggactaac tactgtatta aataacaccg taaataagtt
      721 caatagttta gggtttagcg gagtttcctt tgttagacaa gacaattaca tattagtagt
      781 aatatcgact aacactcaca gccatggaat acagacaata tttgtagata atgcaggaac
      841 atcaactaac ataactacta aatctgtaaa cagtcttaaa aataacagta gttaccaata
      901 cagtataaac tactatgatg agtatggaag accttttcca atagttacag gagataagtt
      961 tgtatttgaa accgatccat tatctaacac agcagggcta ctgccccaag cttctataac
     1021 aatagagtca gaccctccag aaggagcctc ttattataga ataggactta cagaaaataa
     1081 aactactgat aagtacatca cgttagtggg aaaagtagct tccacaacta atgacgacta
     1141 tataatcata gacttaaaat ctttagagta
//
LOCUS       DATGSI010000296         4552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_681889, whole genome shotgun sequence.
ACCESSION   DATGSI010000296 DATGSI010000000
VERSION     DATGSI010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681889"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1006
                     /locus_tag="VK031_04505"
     CDS             206..1006
                     /locus_tag="VK031_04505"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HLS53211.1"
                     /translation="MVTVEELLNLPVLRRLKLLAGKKGLNRRVSCLTVMEVPDVIRWL
                     RGNDFLITSLYALKDDVNKQLELIDKLADCNCSCLAVKLGQYVKELDPSILKKADERG
                     LVLVEIPRDMTYIEIIVNGMEKIIHNRDIVYIVEKYMKDIILDNYKDDNIMIERGELL
                     GFKINEGYSLAMTLLDQNSDLEKINKLRRLAKEVAKKSDVILKFTYNPVVTIDGKSTI
                     LLFSNKKAEISNNLDKIIDIALDYLNREGFKDVRLGIGSLDQGLEGIK"
     gene            1036..1374
                     /locus_tag="VK031_04510"
     CDS             1036..1374
                     /locus_tag="VK031_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLS53212.1"
                     /translation="MSNILQQKTNVYHYEDLKMYLLLNEVFKNKDYEIFDDIEDKLGQ
                     DLIMTLEKFYESNMDIRQTANNLYVHQNTVRYRLKKVFELTGYDINKFEDNFKLYLFL
                     FYKRVKGLRG"
     gene            1536..2033
                     /locus_tag="VK031_04515"
     CDS             1536..2033
                     /locus_tag="VK031_04515"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HLS53213.1"
                     /translation="MTFEKYLEYLKYIMDRFHPIGSNEEGGVTRLGYSKEEDLMHWEF
                     MRIAKEEGFQVETDQVGNSYASLGKYDEYHLVGSHLDSVINGGRYDGVIGVAVGLLLL
                     KIVKEKALDIPLKVVAFRCEESSNFMKSTIGSGLIVGENYEKEFDTLVSRSGEGLREI
                     FAKRG"
     gene            2049..2741
                     /locus_tag="VK031_04520"
                     /pseudo
     CDS             2049..2741
                     /locus_tag="VK031_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
     gene            3228..>4552
                     /locus_tag="VK031_04525"
     CDS             3228..>4552
                     /locus_tag="VK031_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HLS53214.1"
                     /translation="MKKILNYVPGFRSNKMWKKILASIYYLFSFFAASADVGVFLFLL
                     SMPFFIFSLAEVIRSRKKRNINKLTVITLTLSLVLLITGAANIESVEDDLSNGDKAQA
                     EKTELSEETATQDKSEQDVEDKDLEDSKDVEEGLNEEDLIEEDKDGEKPSQQTEEKPK
                     ATGSLKVHYINVGQGDSIFIQLPNGETGLIDGGTRGSGSTVINYLNSQGVKKLDYIIA
                     THPHEDHIGGLVAVIDRFNIGKVYMPEKTHTTKVFEDLLLAIERKNKKITRARAGDTI
                     LNGNGINMRILAPGSNASGSNLNNYSVVIKLDFKNNSFMFTGDAESQTENYLVNSGQN
                     LKTDVLKVAHHGGNTSSSAGFLNKAKPKYAIISCGKNNQYGHPHREVVNRLESIDATI
                     YRTDLDGTIVATSNGNTISFNKKASVSKGDSVASQPGSKSSQKPKPQPKP"
BASE COUNT         1696 a          547 c          978 g         1331 t
ORIGIN      
        1 aaaacaattg ttattaatac cggatatagg ccagatatga atggaatgcc ttaagatatt
       61 acaaggttag ttatgaatta taggaaattt caaagctaca gaatcttctg tagttttttg
      121 tatttatttt aaattgagca aatatttcta aagttatagc aattatcatt gcggatggtt
      181 ttcaacctat tgagaggggg ataaaatggt tactgtagag gaacttttga atttacctgt
      241 acttagaaga ttaaaattat tagctggcaa aaaggggtta aataggaggg tctcttgttt
      301 aactgttatg gaggttccag atgttataag atggttaaga ggcaatgatt ttttaattac
      361 cagtctctat gcattaaagg atgatgtgaa taagcagtta gaattaatag ataaattagc
      421 agattgtaat tgttcctgct tggctgtcaa actaggtcaa tatgtcaaag aattggatcc
      481 atcaattctg aaaaaggctg acgagcgggg cttggtatta gtagagatac caagggatat
      541 gacatatata gagattatag taaacggcat ggaaaaaatt atacataata gagacatagt
      601 atatatagtt gaaaagtata tgaaggacat aatcttggac aattataagg atgacaatat
      661 aatgatcgag agaggggaac tattaggctt taaaattaat gagggatatt cgttggcaat
      721 gaccttatta gatcaaaata gtgacctaga gaaaattaac aaacttagga gattagccaa
      781 ggaagtggct aagaaaagcg atgtaatttt gaagtttacc tataatccag tggttactat
      841 cgatggaaag tccactatac ttcttttttc aaacaaaaaa gcggaaattt ccaataattt
      901 agataagatt attgatatag ccttagatta tctaaataga gaagggttta aagatgtaag
      961 gctgggaata ggttccttag atcaaggttt agaagggatt aaataagcta ttatagttca
     1021 ttagaagtgt taaggttgtc aaatatatta caacagaaaa caaatgtata tcattatgaa
     1081 gatttaaaaa tgtatctttt actaaatgag gtattcaaaa ataaggacta tgaaattttt
     1141 gatgatatag aagacaagct aggacaagat ctaattatga ctctggaaaa attttatgaa
     1201 tccaatatgg atattaggca aacagcaaat aatctgtatg ttcatcaaaa tactgttcga
     1261 tacaggttaa agaaggtttt tgaacttaca ggttatgata taaacaaatt tgaagataat
     1321 tttaaacttt acctattttt attttataaa agggttaagg gattaagggg atgattcatc
     1381 ctagtcaaca attactcact tttctaagaa acagaaggtt tacaaaaaag ggctaagatt
     1441 tttgtagcct cctacatatg taatttcaga taattatgat aatataaaaa tgaactggaa
     1501 aacattttcc cgaataaggt tacagaggtg gagcaatgac atttgaaaaa tatttggagt
     1561 atttaaaata cataatggat aggtttcatc ctatcggctc aaatgaagaa ggtggggtaa
     1621 ctagattagg atatagcaag gaagaagacc tgatgcactg ggaatttatg aggatagcaa
     1681 aggaagaagg atttcaggtg gaaacagatc aggtaggcaa ttcatatgct agtctaggta
     1741 aatatgatga gtaccatttg gtaggttccc atttggattc ggtgataaat ggcggaagat
     1801 atgacggtgt tataggtgta gctgttggct tattattact aaagatagtc aaggagaagg
     1861 ctttagatat acctttaaaa gtagttgctt ttagatgtga agaatccagt aattttatga
     1921 aaagcaccat aggtagtggc ttaattgttg gggaaaatta tgagaaagaa ttcgacactt
     1981 tagtttcccg ttcaggggaa ggtttaagag aaatatttgc taagagagga taaagccaaa
     2041 gacctaaaaa aataagggga gttaaatctt ttctagaact tcatattgaa caaggaagaa
     2101 tattggaaga agaaggtcta actttaggaa ttgttaacgc aatagcagga aatattaggt
     2161 taaaagttaa tatagaaggt ttagcagagc attctggcgc tacccctatg gatttaagaa
     2221 aggatgcttt atgttgtgct gcagaaatca ttttaggcct agagtcatta gctaagtcag
     2281 ataaagagag tagtgccgtg attacagtgg gaagcatata caatctacca aactcggtaa
     2341 acgtgatacc aggaaaggta gaattttcag tggacataag ggataagtca aatgagaata
     2401 tgcaggaatt aaagagaaag gtgaaaaaat tagtaaaatc tatcagcact tttcgaggct
     2461 tggactttag tatagagacg atatccaatg ctgaggctgt agtgctagat agtgatttaa
     2521 ataaggagtt agtaaagata gcagaaaacc ttaatatcaa aaacaaggtt atgcccagtg
     2581 gtgcaggcca cgatgctatg aagatggcca agataactag gacaggactt attttcattc
     2641 catgtaaaga aggaattagc cataatccta gggaaagtgc aagattagaa gatgctgtat
     2701 acggtggtat ggtaattttg gattatttaa gaagtagtta aagattttaa tctttatgta
     2761 gaggtaggaa aatactaaat agtaattaga taactggtat aaatcatatt gaatgagaat
     2821 taaaattaat gatataattg taataaggag attggtaata gtcttgtaag acagttagaa
     2881 attaaggtaa atacagttaa tacatatgat gagatgttgg aaaaggaagg tagctgataa
     2941 gggtaaatta tattttcttc aatttgataa accattgcat aatattttgg tctaattgag
     3001 tataatatta tccccaatga agaactaaaa aggtatatga aaacctgtga aaaactccct
     3061 gtaagaagga tatttaagaa taagttagaa aagaatgtag tatttaattc tgtggtagga
     3121 tttgatgata taagaagtta tatataagga tctggagcca gaggctcctt ttttattttg
     3181 cttttttgca agaaggactt aaattgaaag tatgagagga agattaaatg aaaaagattt
     3241 taaactatgt tccaggattt agaagcaata agatgtggaa gaagatttta gcaagtattt
     3301 actacttatt ttcctttttt gctgcttcag cagatgtggg agtattttta tttttactat
     3361 ccatgccttt ctttatattt tcattggctg aagtaattag atcaagaaag aaaaggaata
     3421 taaacaagtt aactgtaatt accttaacct tatccttagt tcttttaatt actggggcag
     3481 caaatattga aagtgtggaa gatgatttat ccaatgggga taaagcccag gcagaaaaga
     3541 cagaattaag tgaagaaact gctacccaag ataagtcgga acaggatgta gaagataaag
     3601 atctggaaga ttcaaaagat gtggaggaag gcttaaatga agaggattta attgaagagg
     3661 ataaagatgg tgagaaaccc agtcagcaaa cagaagaaaa gccaaaggct acaggtagct
     3721 taaaggttca ctacataaat gtaggccaag gtgattcaat cttcattcaa ttgcctaatg
     3781 gagagactgg cctaatagat ggtggtacta gagggagtgg ctctactgta atcaattatt
     3841 taaatagcca gggagttaaa aaactggact atataatagc cacccacccc cacgaggacc
     3901 acataggtgg cctggtggca gtaattgata gatttaacat aggtaaggta tatatgcctg
     3961 aaaaaaccca taccactaaa gtatttgaag atttactttt ggcgattgaa aggaagaata
     4021 aaaagattac cagggctagg gctggggaca ctatccttaa tggaaatgga attaatatga
     4081 ggatcctagc gccaggctcc aatgccagtg ggtcaaattt aaataattat agtgtggtta
     4141 tcaaactaga ctttaaaaat aatagcttta tgtttactgg agatgcagaa agccaaactg
     4201 aaaattactt ggttaatagt ggacaaaatt taaagacaga tgttttaaag gtggcccacc
     4261 atggtggaaa cacatcttca agtgctggct ttttgaataa ggccaagccc aagtatgcaa
     4321 taataagctg tgggaaaaac aatcaatatg gccatcctca tagggaagtg gtcaatagat
     4381 tagaaagcat tgatgcaacc atatatagaa ctgatttaga tggaacaatt gttgccacat
     4441 caaatggaaa tactataagt tttaataaaa aagccagtgt aagcaaaggc gattctgtgg
     4501 ctagccagcc aggatcaaag tcaagtcaaa aacctaaacc acaacctaaa cc
//
LOCUS       DATGSI010000297         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_349786, whole genome shotgun sequence.
ACCESSION   DATGSI010000297 DATGSI010000000
VERSION     DATGSI010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349786"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VK031_04530"
     CDS             <1..586
                     /locus_tag="VK031_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HLS53215.1"
                     /translation="KPEALLKLIILSSSNEDSIVADFFAGSGTTAAVAEKHKRNWVIS
                     DLEDIAVATIRKRLSQVGKSSYQVLSKSQAKSKINFEIEREAKNQKEYLKFIFKEYKM
                     DWEGVELKEEDREIIEEILKKDSLSLIDYIGIGYKNNKGQNIIKKEIIRRRDDISSHY
                     HVKYLLDEEFKGQVYIKFIDIFGRNFYEIRKINK"
     gene            672..1025
                     /locus_tag="VK031_04535"
     CDS             672..1025
                     /locus_tag="VK031_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HLS53216.1"
                     /translation="MKEMENRVETEDREEGLGIEEYDHEVDPDIIYLYFDDDVEVECV
                     VLGVFEVEDKEYIALLPESEEEILLYEYRENSEDFELIPIKDEEELQSVYEAYYVLFE
                     EEADELDEEDDLDIE"
     gene            1087..1920
                     /locus_tag="VK031_04540"
     CDS             1087..1920
                     /locus_tag="VK031_04540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HLS53217.1"
                     /translation="MYKLIALDLDGTLLNDEKNIAEENIRLLNQLSKNGIKILIATGR
                     GYYSAKQLTKNIKGDLIFVANNGGIVRQRENDQVLFSQFLDEAHCKLVLKEGLKRDLH
                     PIIHIDYFLDGYDIILSNKFIKRYKDSAYVKKVTRYKLMDKVLDEKIDRILALVYPGG
                     KDKLNNFNLYINENYPDRFSSHIMENVEIDEGILELMNPSVNKWEGIKKYARSQGIKE
                     EEIVVIGDDNNDIDMIRNAGLGIAMKNGTALARSIADTISEKDNNQAGVAFELKRVLN
                     I"
     gene            1942..>2592
                     /locus_tag="VK031_04545"
     CDS             1942..>2592
                     /locus_tag="VK031_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589008.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HLS53218.1"
                     /translation="MSDITVYRTYSSYLKKRFGERIYKLPVNLPLTCPNRDGYLGRGG
                     CTFCGEEGGAFGELPRFTPVQDQLKRNKAYIGKRYKANKFIAYFQNFSNTYLPLDKFK
                     AYMKEAIMEDVVGISVSTRPDCINDLYLDFLQNLKEKYNLEITVELGLQTVNYHSLKQ
                     VNRGHGLAEFIDAVLRIKKHKLRTCTHLILNLPWDTMDDTIENAKVLSSLGVEEVKL"
BASE COUNT         1023 a          281 c          543 g          745 t
ORIGIN      
        1 aaagccagag gccctattaa agcttattat cctatcttca tccaatgaag actcaatagt
       61 ggcagatttt ttcgcaggga gtggaaccac agcagcagtg gcagaaaagc ataagagaaa
      121 ctgggtaata tcagatcttg aagatattgc tgtagcaacc attagaaaaa gactatccca
      181 ggttggaaaa tcatcctacc aggtgttgag taaaagccag gctaaaagca aaataaattt
      241 tgaaatagaa agggaagcta aaaatcaaaa ggagtattta aaatttattt ttaaggaata
      301 taagatggat tgggaaggtg tagaacttaa ggaggaagat agagaaataa ttgaagaaat
      361 tttaaaaaaa gattccttat ctttaataga ttatattggg atagggtata aaaataacaa
      421 gggtcaaaac attataaaaa aagaaataat tagaagaaga gatgatataa gtagccatta
      481 ccatgtaaaa tatctactag atgaagaatt taaaggccaa gtatatataa aatttattga
      541 tatatttggt agaaactttt acgaaataag aaaaataaat aaatagaatg gtatttaatc
      601 aaatgtataa taataaagta taaaggagag tagttagtag ataagaacag gaaattagaa
      661 ggaggatggc catgaaagag atggaaaata gagtagagac tgaagataga gaagaaggtt
      721 tagggataga agaatatgat catgaagtgg atccagatat tatttacctt tactttgatg
      781 atgatgttga agtagaatgt gttgttttag gagtatttga ggttgaagat aaggaatata
      841 tagccttgct tccagaaagt gaagaggaga ttttacttta tgaatatagg gaaaatagtg
      901 aggattttga attaattcct attaaggatg aagaggaact acagtcagtt tatgaggcct
      961 attatgtact gtttgaggaa gaggctgatg aattagatga ggaagatgat ttagatatag
     1021 aataaacaaa aataaagtac tttgcgcctg caaggtgctt tatttctaat aagggggaaa
     1081 agtattttgt ataaacttat agccttagac ctagatggaa ccttgttaaa tgatgaaaag
     1141 aatatagccg aggaaaatat aaggctatta aaccagctat ctaaaaatgg aattaaaatt
     1201 ttaattgcaa ctggtcgggg ttattattct gcaaaacaat tgactaagaa tattaaggga
     1261 gatctcatat ttgttgcaaa caacggtgga atagttcgtc aaagggaaaa tgatcaggtc
     1321 ttatttagcc aatttttaga tgaagcccat tgcaagctag tactaaagga aggtctaaaa
     1381 agagatttgc accctataat tcatattgac tatttcctgg atggttatga tataatatta
     1441 agcaataagt ttattaaaag atacaaagat agtgcctatg taaaaaaggt tactaggtat
     1501 aaacttatgg ataaggtttt ggatgaaaaa attgatagaa tcttggccct agtatatcct
     1561 gggggtaagg ataagctaaa taattttaat ttatatataa atgaaaatta tcctgataga
     1621 tttagtagtc atataatgga aaatgtggaa atagacgaag gaatattaga acttatgaac
     1681 ccttcagtca ataaatggga aggtataaaa aaatatgcaa ggtcccaggg aattaaagaa
     1741 gaagaaatag tagttatagg cgatgacaat aatgatatag atatgataag aaatgcaggc
     1801 cttggtatag caatgaaaaa tgggactgcc ttagctaggt ctattgcaga tacaatatcg
     1861 gaaaaagata ataatcaagc aggtgtggct tttgaattaa aaagggtatt aaatatataa
     1921 attacccatg gggtgaatta attgagtgac attacagtgt ataggactta ttcaagttat
     1981 ttaaagaaac gatttggtga gaggatatat aaattacctg tcaatctacc acttacttgt
     2041 cctaataggg atggatattt aggtaggggt gggtgtacat tttgtggaga agaaggggga
     2101 gcctttggag aactgcctag gttcacccct gttcaagatc aactaaaaag aaacaaggcc
     2161 tacattggaa aacgttataa ggccaataaa tttattgcat atttccaaaa tttttcaaac
     2221 acttacctgc ccttagataa atttaaggca tatatgaagg aagcaataat ggaagatgtg
     2281 gtaggtattt cagtatccac aaggccagat tgtattaatg acttatatct agacttcctt
     2341 caaaacttaa aggaaaaata taatttagaa attactgtag agttaggctt acaaacagtt
     2401 aattatcaca gtttaaaaca ggtcaatagg ggacatggtt tggccgaatt tatagatgca
     2461 gtgttaagaa taaaaaaaca caaattaaga acttgtaccc atttaatctt aaatttacct
     2521 tgggatacta tggatgatac aatagaaaat gctaaggtac tttcctcttt aggagtagaa
     2581 gaagtaaaat ta
//
LOCUS       DATGSI010000298         5475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_211421, whole genome shotgun sequence.
ACCESSION   DATGSI010000298 DATGSI010000000
VERSION     DATGSI010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211421"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1619
                     /locus_tag="VK031_04550"
     CDS             288..1619
                     /locus_tag="VK031_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HLS53219.1"
                     /translation="MEKDKMQGNGQFQSRWGFILACVGSAVGMANVWGFPYKLGSHGG
                     GAFLIAYLIFIWIFSYVGLSAEYAIGRRARTGTVGAYENAWTSGNKSTIGKIVGWLPL
                     AGSMCIAIGYAVIISYVLKGWTQAVTGSLMTVEPGPWFESFAFTDYSVIGFHLIVVIG
                     TLLTLTLGADSIEKTNKVMMPLFFILFVVLAIRVAFLPGAIEGYKFMFTPRWSELKDP
                     MVWIWAMGQAFFSLSVTGSGMIVYGAYLSDEEDIVDAAKSTAIFDTIAALIAALVIIP
                     ATFAYGMDVGSGPGLLFVTLPTILQNMPFGRIFAIILFTAVVFGGVSSLQNMFEVVGE
                     SLMYKFPKLKRLPMLVILAVITFGVGVNMETIEKWGPWMDLVSIYIIPIGATIGAISW
                     FWIMKKEDILDEINLGAKKKQGDLWYNIGRFVYVPFATILCLVALIKGIAF"
     gene            1744..2412
                     /locus_tag="VK031_04555"
     CDS             1744..2412
                     /locus_tag="VK031_04555"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLS53220.1"
                     /translation="MINKKTLSDQIYYILKMEILKSKIPFGSKLTNRALQKRFGVSSS
                     PVRDAINRLYQDGLVKSIDTTGATVVDFNYEFFLEVNEILLYVVNTGVKLSYEKSDTR
                     EICKHLKKYLELQEKHIGSDQFFDYDYKFHKTFIDYSNNSRLTKIFKEYNVLHEMLVR
                     NFYNPETMQIQENSVKTHREIMDAFCRGDYRLAMDLTEKHYEGAEEIFKNILQRRDKS
                     RLKK"
     gene            2550..3590
                     /gene="spoIID"
                     /locus_tag="VK031_04560"
     CDS             2550..3590
                     /gene="spoIID"
                     /locus_tag="VK031_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HLS53221.1"
                     /translation="MKKLNYYLLSVLIITILIPSVIVKAFNIVSYPSLLEGKEIEKAE
                     KPSEKKEAGPIKRGDWKIDNVKVYNPDNKKTESLQLDEYIKGVVAAEMPARFHMEALK
                     AQAVAARTYAISRSLKYKSGHKDHKEAPLCSSVHCQAYLNLKQLKEIHGEKWIKDYWG
                     KIEEAVESTAGEIIYYGDQIIEPLYHSTSGGKTEDAIHVFATDSPYLKSVDSPFEEEA
                     PKYREVITFTADEFIKTINKEYKDAKLDKKNFFEKIKLIEKTPTGRVKKLSINKVIVN
                     GRDIRELFELNSTNFTITYDKRLDLIEIETYGYGHGVGMSQWGANGMAKKGSNYIEIL
                     KHYYSGIEVGKK"
     gene            3632..4456
                     /locus_tag="VK031_04565"
     CDS             3632..4456
                     /locus_tag="VK031_04565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLS53222.1"
                     /translation="MSLEIISTRGEEMKDKLSKFLKENGFLLFLFACVCIVAVSTIFI
                     LTKEVGNPREDELVILDQKKNQMEDKSEDKKEVHQDKEEEEELPDKEKGQDMAKIEKE
                     DNPQDKLLEDQSKEVFADEGLEEEIEFIEDVVTDNVATREKFILPIDGEIITKFTDDT
                     LVYSETLSEWRAHLGIDIRGQVGSIIKAAADGVVKEVYEDPLWGNVVLIDHGNGLFTK
                     YANLGNKSMVEPGLKVRQGEEIGTIGETANIEMLMEPHLHFEAIKDGKNVDPRSIR"
     gene            4511..4765
                     /gene="spoIIID"
                     /locus_tag="VK031_04570"
     CDS             4511..4765
                     /gene="spoIIID"
                     /locus_tag="VK031_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HLS53223.1"
                     /translation="MKDYIEERTLEIAEYILSERATVRQAASVFGVSKSTVHKDVTER
                     LPKINPLIAKAVKEILEINKAERHIRGGEATKLKYKAMNE"
     gene            4881..>5475
                     /locus_tag="VK031_04575"
     CDS             4881..>5475
                     /locus_tag="VK031_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HLS53224.1"
                     /translation="MRFCLNIFGRVVFVVFRADIGIDLGTSSVLVYVKGKGIVLKEPS
                     VVAIDQYTDKFLAVGEGAREMLGRTPGNITAIKPLKNGVISDYEITQRMLKYFFQKAI
                     KRYIFKPRVVICVPSGITEVEKRAVVEASNNAGAIRTYIIEEPIAAAIGADIDITEPH
                     GNMIIDIGGGTTDIAVIALGGIVVNRSIKVAGDDFDNA"
BASE COUNT         1989 a          741 c         1193 g         1552 t
ORIGIN      
        1 ttaacaattc caagaagagt ctatacttac agacatatgg acgttgttgc agattcagta
       61 atcagactat atgaaaatag ggatcaaatt aggccattaa aatttgttta tgagccagca
      121 caattaagat tctttacagc aaagtttgat tatgcagact aattagattt acttttgtcc
      181 atatattggt ctggaccaat atatggacaa tttataagta aaatagtaag aaaattaaaa
      241 atattgggaa atacaaatcc caattagtac tttaaaagga ggcaagaatg gaaaaagata
      301 agatgcaagg taatggtcaa tttcaatcta ggtggggatt tatcctagcc tgtgtaggtt
      361 cagcagttgg gatggcaaac gtttggggct ttccctataa gttaggtagt cacggtggag
      421 gggcctttct tatagcctac ttaatattca tatggatatt tagttatgtt ggtctttctg
      481 cagagtatgc cattggtaga agggccagaa cagggacagt aggggcctat gaaaatgcat
      541 ggacatctgg aaacaaatca accataggta agatagtagg atggttgcct ttggcagggt
      601 ccatgtgtat tgctatagga tatgcggtaa ttatatcata tgtactaaaa ggttggacac
      661 aggctgtaac aggcagtctg atgacagtag agccaggtcc ttggtttgaa tcctttgcct
      721 ttacagatta ttcggtaata ggctttcatt taatagtggt aataggaacc ctattaacct
      781 tgaccttagg tgcagatagt atagaaaaga caaacaaggt tatgatgcca cttttcttta
      841 tattatttgt agtcctagca ataagggtgg cctttttacc aggagcaata gagggttaca
      901 agtttatgtt tactcctaga tggtctgagc taaaggatcc tatggtttgg atctgggcca
      961 tggggcaggc atttttctcc ttatctgtaa caggtagtgg aatgattgtt tacggtgcct
     1021 atttatctga tgaagaggat atagtggatg ctgccaaaag tacggcaata tttgatacaa
     1081 tagcagcttt aatagcagcc ttagttatta ttcctgctac ctttgcatac ggtatggatg
     1141 taggttctgg tccaggactt ttgtttgtaa ccttgcctac aattctacaa aatatgcctt
     1201 ttggtaggat ctttgccata atacttttca ctgcagtagt atttggtgga gtatcctcat
     1261 tgcagaatat gtttgaggtt gttggcgaat ccttaatgta taagtttcca aagctaaaaa
     1321 ggcttcctat gttagttata ctggcagtta ttacttttgg tgtaggagta aatatggaaa
     1381 caattgaaaa atggggcccg tggatggatt tagtatctat ttacataatc cctataggag
     1441 caactattgg agcaatttct tggttctgga taatgaaaaa agaggatatt ttggatgaaa
     1501 tcaatcttgg agccaagaaa aagcaaggag atttatggta caatatagga agatttgtct
     1561 atgtaccttt tgctacaata ttatgcttag ttgctttaat taagggaata gctttttaat
     1621 attatattaa attgagagaa aagaggtggt taaagccacc tcttttattt ttatatcttt
     1681 aatgttataa tactatagta gactaactaa ataagatata ttataacaga caggagcagt
     1741 atggtgatta ataaaaaaac tttaagtgat caaatttatt atatattgaa aatggaaata
     1801 ttaaaaagta aaattccttt cggttcaaag ctgactaatc gggctttaca aaaaagattt
     1861 ggggttagct cttcgccggt aagagatgct attaatcgtt tataccagga cggattagta
     1921 aagtcaatag atacaactgg tgctacagta gtagatttta actatgaatt cttccttgaa
     1981 gttaatgaaa ttcttctcta tgtggtaaac acaggggtta agttatccta tgaaaaatct
     2041 gatacaaggg aaatatgtaa acatttgaag aagtacttag agttgcaaga aaagcatata
     2101 ggaagcgatc agttcttcga ttatgattat aaattccata aaacatttat agactactca
     2161 aataattctc gacttacaaa aatatttaag gaatataatg ttttacacga aatgttagta
     2221 agaaatttct acaatcctga aactatgcaa atacaggaaa atagcgttaa aactcacaga
     2281 gaaataatgg atgccttttg tagaggagat tatagattgg ctatggattt gacagagaaa
     2341 cattacgaag gggcagaaga aatatttaag aatattttac agcggagaga taaaagtaga
     2401 ttaaaaaaat aaacttaaaa gttccttgga caatagtcca gggtttttta tgtatataaa
     2461 agataggaag ggagaaggtc aaaagcttgc ataatattcc ccaagcttga ataagtatat
     2521 cttatatact gaagagatga ggggaatata tgaagaagtt aaattattat ttactttcag
     2581 tcttaataat aaccatatta attccatcag ttattgttaa ggcttttaat attgtatcct
     2641 acccaagtct tcttgagggc aaagaaatag aaaaagcaga aaaaccttca gaaaaaaaag
     2701 aagcaggccc aataaaaagg ggagattgga aaatagacaa tgtgaaggta tataatcctg
     2761 ataataaaaa gacggaaagc ctccagttgg atgaatatat aaaaggtgta gtagcggcag
     2821 aaatgccagc aaggtttcat atggaggcct taaaggccca ggcagttgca gccagaacat
     2881 atgccatcag tagaagttta aaatataaat caggccataa ggatcacaaa gaagctccct
     2941 tatgtagcag tgtacattgc caagcatacc taaacttgaa acagttaaag gagatccatg
     3001 gagaaaagtg gataaaggat tactggggaa aaattgaaga ggcagttgag tctacggcag
     3061 gggaaattat ctattatgga gaccaaatca tagagccttt atatcattca accagtggcg
     3121 ggaaaactga ggatgcgata cacgtttttg caacagattc tccataccta aaatcagtag
     3181 acagcccctt tgaagaggag gcgcctaaat acagagaagt aattaccttt actgcagatg
     3241 aatttataaa aacaataaat aaggaatata aagatgcaaa gctagacaag aaaaactttt
     3301 ttgaaaagat aaaactaatt gaaaaaaccc ctactggacg tgtaaaaaaa ctttccataa
     3361 ataaggttat tgtaaatgga agggatatta gggaactatt tgaactaaac tctactaatt
     3421 ttactataac ttacgacaaa aggctagatc taatagaaat agagacctat ggctatggcc
     3481 atggagttgg catgagccaa tggggtgcca atgggatggc taaaaaagga agtaactata
     3541 tagaaatatt aaaacattat tattcaggga tagaagtagg gaaaaagtga cctaaaaagg
     3601 tcactttttt tattttccag gtattaaaaa tatgtcactg gagataatat ctactagagg
     3661 tgaggaaatg aaggataagc tatcgaaatt cttgaaagaa aatggatttt tattatttct
     3721 atttgcctgt gtatgcatag tagcagtaag taccatattt atattaacca aggaggtagg
     3781 aaatcctagg gaagatgaac tggtcatatt ggatcaaaag aagaaccaaa tggaagataa
     3841 gtctgaagat aaaaaagaag tgcatcagga taaagaagaa gaggaagagt tgcctgataa
     3901 agaaaaaggg caggatatgg ccaagattga gaaagaagat aatcctcaag ataagctact
     3961 agaggatcag tctaaagagg tatttgcaga tgaaggacta gaagaagaaa tagagtttat
     4021 tgaagatgta gtaaccgaca atgtagcaac aagagaaaaa tttatactcc ctatagacgg
     4081 ggaaataatt actaaattta cggatgatac cttagtttat tcagaaactt tatctgaatg
     4141 gagagcccat ctaggtatag atattagggg acaggtagga agtattatta aagctgcagc
     4201 tgatggtgtt gtaaaagaag tatatgagga ccctttatgg ggaaatgtag ttttaataga
     4261 ccatggtaat ggtttattca caaagtatgc caaccttgga aacaaaagta tggtagagcc
     4321 tggtttaaaa gtaagacagg gagaagaaat aggaaccata ggagaaacgg ctaatattga
     4381 aatgcttatg gaaccacact tgcactttga agccataaaa gatggaaaaa acgtggatcc
     4441 acgttctatt agataactag aaagcataag tattaattag aatacccaat agagagaagg
     4501 gggttattga gtgaaagatt atatagagga aagaacgtta gaaatagcag aatacatact
     4561 aagtgaaaga gctactgtaa ggcaagccgc cagcgtattt ggtgttagta aaagtactgt
     4621 tcacaaggat gttactgaaa gattacccaa gataaaccct cttattgcaa aagctgttaa
     4681 agaaattcta gagatcaaca aggctgaaag gcatatcaga ggtggggagg caactaaact
     4741 aaaatataaa gcaatgaacg aataagagcc caactttggg ctttttagtt ttttgtaaag
     4801 gaagaatttt ctaatatatg gaataaaaca tttgcagaat agcctaaaga gttgtaaaat
     4861 aatagtaaat gcagatacat atgcggtttt gcttaaatat atttggaagg gtggtttttg
     4921 tggttttcag agcagatata ggaatagatt taggaacatc cagtgttctg gtttatgtga
     4981 aaggaaaagg aatagtactt aaggagcctt ccgtagtagc cattgaccaa tatactgata
     5041 aatttcttgc agtaggagag ggagcaaggg aaatgctagg gagaacgcct ggcaatatta
     5101 cagccatcaa accgttgaaa aatggcgtaa tatctgacta cgaaataaca caacggatgc
     5161 taaagtattt tttccaaaag gccataaaga ggtacatatt taaacctaga gtagtaattt
     5221 gcgtccctag tggaatcact gaagtggaaa aaagggcagt tgtggaggca agtaacaatg
     5281 caggtgccat aagaacctat attatagaag aacctatagc tgctgccata ggagctgaca
     5341 ttgatattac tgagccacat ggaaatatga ttatagatat aggcggaggc accactgata
     5401 tagctgttat agccctagga ggtattgtag taaaccgttc cataaaggtg gctggagatg
     5461 atttcgataa tgcca
//
LOCUS       DATGSI010000299         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_117135, whole genome shotgun sequence.
ACCESSION   DATGSI010000299 DATGSI010000000
VERSION     DATGSI010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117135"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VK031_04580"
     CDS             <1..296
                     /locus_tag="VK031_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HLS53225.1"
                     /translation="LEETDKIMKAQMARGADFESGNILERAKSLVPLLVPLFVNAFRR
                     ADELATAMEARCYRGGENRTRLNELKLDRTDIIVFIIMLGFFSFIISTRYIGS"
     gene            298..1032
                     /gene="truA"
                     /locus_tag="VK031_04585"
     CDS             298..1032
                     /gene="truA"
                     /locus_tag="VK031_04585"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HLS53226.1"
                     /translation="MRNIKLLIQYEGTAYAGWQRQDNALGIQEVIEEAIKKVTGEEVR
                     LIGSGRTDRKVHALGQVANFHTSSQIPGHKFKYALNIKLPEDIQIIKSKEVDKNFHSR
                     YDAKGKRYKYIIYNRPLANPIYRNFAYHENRKLDINKMERAARHFIGSHDFKSFMGPR
                     TGTVNTIRTISKIDMRKENELIYITIEGNSFLRHMIRIIVGTLVYVGIGKFEEQDIPK
                     IIEARDRTKAGPTAPPQGLFLEKVYY"
     gene            1195..1623
                     /gene="rplM"
                     /locus_tag="VK031_04590"
     CDS             1195..1623
                     /gene="rplM"
                     /locus_tag="VK031_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HLS53227.1"
                     /translation="MKSYMAKKNEIERKWYVIDAENKVLGRLASEVSTLLTGKHKPIY
                     TPHVDTGDFVIIINADKVKLTGKKLNQLNHVYHTGYPGGLKEVPYRRLMEENPERIIE
                     LAVKGMLPKNKIGRQMFRKLKVYRGPEHKHEAQQPEIYEF"
     gene            1649..2041
                     /gene="rpsI"
                     /locus_tag="VK031_04595"
     CDS             1649..2041
                     /gene="rpsI"
                     /locus_tag="VK031_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HLS53228.1"
                     /translation="MAVEQYMGTGRRKTSVARVRLVPGNGKFLINNVDIEEYFDFETL
                     RSIAREPLEITETMGKFDVLVNVHGGGFTGQAGAIRHGIARALLEVDEDLRPTLKRAG
                     HLTRDPRMKERKKYGRKKARKSPQFSKR"
     gene            2243..>2610
                     /locus_tag="VK031_04600"
     CDS             2243..>2610
                     /locus_tag="VK031_04600"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLS53229.1"
                     /translation="MKVIFISKRTIILFFLVIILALLAIKSFPKKEAAPVSHIPATPV
                     LNKTIVIDPGHGGADPGALGSKDSREDELNLEISLRLKRIIEKSGGIVVMTRESSEGL
                     YTEESKTLREKKIEDLNNRK"
BASE COUNT         1029 a          353 c          557 g          671 t
ORIGIN      
        1 tattggaaga gacagacaag ataatgaagg cacaaatggc cagaggggct gattttgaat
       61 caggaaatat attggaaagg gctaaaagtt tagttcccct tttagttccc ctatttgtaa
      121 atgccttcag aagggcagat gaattggcaa cagccatgga ggccaggtgc tatagaggcg
      181 gagaaaatag gaccaggtta aatgaattga aattagatag gaccgatatt atagttttta
      241 taatcatgtt aggctttttt tcatttataa tttcaacaag gtatattggg agttagtatg
      301 agaaatataa aacttttaat acaatatgag ggaactgcct acgccggatg gcaacggcaa
      361 gacaatgcat taggaataca agaagtaata gaagaggcaa taaagaaagt aacaggagaa
      421 gaagtcagac taattggctc aggcaggaca gatagaaagg tacatgccct aggacaggtg
      481 gcaaactttc ataccagttc acaaatacca ggccataaat ttaaatatgc tttaaacata
      541 aagctgcctg aagatataca aataattaaa tcaaaggaag tagataaaaa cttccattca
      601 aggtatgatg ccaaggggaa aagatataag tatataatct acaacaggcc attggctaat
      661 ccaatatata ggaattttgc ctaccatgaa aatagaaagt tagatataaa taaaatggaa
      721 agggcagcca ggcactttat aggtagccat gattttaaaa gctttatggg gccgagaact
      781 ggaacagtca atactattag gacaatcagc aagatagata tgaggaagga aaatgagcta
      841 atatatataa caatagaagg aaacagcttc cttagacata tgattaggat aattgtaggc
      901 accttagtct atgtaggaat aggaaaattt gaagaacagg atattcctaa aataattgaa
      961 gccagagaca ggaccaaggc aggccctact gctccacccc agggtttatt tttagaaaag
     1021 gtatattatt aacatattta taagcgatta ttttaaatgg aataaagggc aaaaaaatgc
     1081 cttgacattg ataaatgcat gtattagaat atactagagt gtttaaaaaa gacccggaaa
     1141 ctttttttat acctcataat atgtaactga tatttggtat aaggagggaa atcgatgaag
     1201 agctatatgg ctaagaaaaa tgaaatagaa agaaaatggt atgtaattga tgcagaaaac
     1261 aaggtactag gtagactagc atcagaagtg tctactcttt taacagggaa acataaacct
     1321 atatatactc ctcatgtaga tactggagat tttgtgataa taattaatgc agacaaggtt
     1381 aaattaacag gtaaaaaatt aaaccagctt aaccatgtat atcacactgg atacccagga
     1441 ggtttaaagg aagttccata tagaagatta atggaagaaa accctgaaag aattatagaa
     1501 cttgcagtaa aaggtatgct tcctaaaaat aagataggta gacaaatgtt tagaaaatta
     1561 aaggtctata gagggccaga acataaacac gaagctcaac aacctgagat atatgaattt
     1621 taagatggat aagaagggag gactgtaagt ggctgtagaa caatatatgg gaacagggag
     1681 aagaaaaaca tctgtagcta gagtaagatt ggtccctggt aatggaaaat ttttaatcaa
     1741 taatgttgat atagaagaat attttgactt tgaaacatta agaagcattg caagagagcc
     1801 attggaaata acagaaacaa tgggcaagtt cgatgtacta gttaatgtac atggcggtgg
     1861 gtttacaggc caagctggag ctataagaca tggtatagct agagccttac tggaagtaga
     1921 tgaagatcta agacctactt taaaaagggc tggacatcta accagagatc ctagaatgaa
     1981 ggaaagaaag aaatacggaa gaaaaaaggc caggaaaagc ccacaattct caaagagata
     2041 atatttataa atattggaaa acagagctgc ttagaaagtg gctctttttt tatgcttaaa
     2101 taggcttgcc ttttagccta atattagata aagtaaggtt ttataggtag aaaatttaac
     2161 tataaaggcc ttaaatctga tttaaagatt agattggaat aaggacatgt ataaagcaat
     2221 atatttctaa agaggtggtc aaatgaaggt aatatttatc agtaaaagaa caattatttt
     2281 attttttcta gtaattattt tagccctttt agccattaag tctttcccaa aaaaggaagc
     2341 agctcctgta agccatatac ctgccacacc tgttttaaat aaaaccatag tcattgatcc
     2401 tggccatgga ggagcagatc ctggagcctt aggcagtaaa gacagcaggg aggacgaatt
     2461 aaacctagaa atttccctaa ggttaaaaag gataatagaa aaaagtggtg gaattgtggt
     2521 tatgaccagg gagtccagtg aaggcctata tacagaggaa tccaagacct taagggaaaa
     2581 aaagatagaa gatttaaaca ataggaaaaa
//
LOCUS       DATGSI010000300         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_211482, whole genome shotgun sequence.
ACCESSION   DATGSI010000300 DATGSI010000000
VERSION     DATGSI010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211482"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="VK031_04605"
     CDS             complement(<1..1770)
                     /locus_tag="VK031_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HLS53230.1"
                     /translation="MFQQYIVFGVIGLTLLLFIDGRIRYEFVSISGVLVLAITGVISP
                     EEVFAGFSHPAVITVASVLVISSALIKSGVIEHLVVLLNGLTDNTYLKVMGLMGVTAL
                     LSAFMNNVGALALILPVALRIAKDSKMSPSKVLMPVSFASLLGGMITVIGTPPNLIVS
                     NYRTQVGGEPFAFFEFAPIGITLTVVGIVFTVILGIRLIPTRKSRNEERLYNIGEYLS
                     EVIVTADSKMVGKRVRDFYEMFKIEVEVLSINRGTHKIIVPKANERLRAGDVLIVKSD
                     SLELTDLIKRTEMRLKGAKLDYLESVPYLKSKDFTLVEVVLRDDSLLIGRTALEVKLR
                     NRYNVNLVAVSRKGILSIGGLKSFRFKAGDILLLQAPVNILQDIYNKLSCLPLAERKV
                     DISMETKKLRQYLPLILFIGGIMLTTFGILPVQISFATVAILLALFKIITPREFYDAI
                     EWPTILLLGTLLPLGGALQSSGASDTIANLLMKLSGILSAKGILVVLMMITMVITNLI
                     SSTATAVLMGPIALSLGKFIGVSPDPFLIGVCIASSSAFLTPIGHQSNMLVMGPGGYK
                     FTDYWRLGLPLSILIIVIGTPLIL"
     gene            complement(1868..>2477)
                     /locus_tag="VK031_04610"
     CDS             complement(1868..>2477)
                     /locus_tag="VK031_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HLS53231.1"
                     /translation="IYASLGYQVLIPDAIYHGERSPLSDYGEESLTKFFWDVVLKNLE
                     ESTDLINSLVNDYEADPEKIGVTGHSMGGFTAAGVFTRQPGLKALVVLNGSCAWEDSN
                     ERFKKTLNLGDIDKRLEAKVKDMDPMNNLDLLKNRPILLLHGDKDGMVPIESQEIFYE
                     KLNRLNTCKEKLSFVKSPGLDHFVTTGMMEKSIKWFHNYVKK"
BASE COUNT          777 a          540 c          363 g          797 t
ORIGIN      
        1 caagattaaa ggagtaccta tgactataat taatatggat aaaggtaatc ccaatctcca
       61 atagtctgta aatttatatc ctccaggccc cataaccagc atgttagatt ggtgacctat
      121 tggcgtcaaa aaggcggagg aagatgcaat acagacccct attaagaaag gatctggaga
      181 gacacctata aactttccta aacttaaggc tataggcccc attagcacgg cagtggctgt
      241 actactaatc aggttagtta taaccatagt aatcatcatt aagactacta aaattccttt
      301 agcagatagg atgcctgaca gtttcattaa tagattggct atggtgtctg aggccccact
      361 ggattgcaag gcaccaccta gtggtagtaa ggttcccaac aataggattg taggccactc
      421 tatagcatca tagaactccc ttggagtaat tatcttaaag agggccaaaa gaattgccac
      481 cgtggcaaaa gatatttgca caggcaatat accaaaggtt gttagcataa tccctccaat
      541 aaagaggatt aagggtaaat attgtcttaa ttttttagtc tccatgctaa tatctacttt
      601 tctttcagct aaaggcaaac agcttaactt attgtaaata tcttgtagga tatttacagg
      661 ggcctgtaat agtagaatat cccctgcctt aaacctaaag gattttaatc ctcctataga
      721 taaaatccct tttcttgata ctgccaccag atttacatta tatctatttc ttaatttaac
      781 ttctaaggct gtcctaccta ttagtaggga atcatcccta agtactacct ccactaaggt
      841 aaaatcttta gattttaaat agggtactga ctctaagtag tctaattttg cacccttaag
      901 cctcatttct gtccttttaa tcaggtctgt taattctaga ctatcagact taacaattaa
      961 tacatcccca gcccttaacc tttcattggc cttaggcact ataattttat gggtccctct
     1021 atttatactt aatacttcca cttctatttt aaacatttca taaaaatctc taaccctttt
     1081 acctaccatt tttgaatcag cagttactat cacctctgat aggtattctc caatattata
     1141 taatctttct tcattcctac ttttccttgt aggtataagt ctaattccta aaataacagt
     1201 aaatacaatg cccaccacag tcaaggtaat acctattgga gcaaattcaa aaaatgcaaa
     1261 gggttctcct cctacctggg tcctataatt tgatactata aggtttggtg gagtacctat
     1321 tacagtaatc atcccaccta acagagaagc aaaggatact ggcataagta cctttgacgg
     1381 tgacatctta ctatccttag ctattctaag tgccacaggc aggattaaag ccagcgcccc
     1441 cacattattc ataaaggcag ataagagagc tgttactccc attaatccca ttaccttaag
     1501 gtaagtatta tctgttagac catttaatag tactaccaaa tgctctataa ctccagactt
     1561 aattaaggca ctgcttatta ctagtactga ggcaactgta ataactgcag ggtggctaaa
     1621 acctgcaaaa acctcctctg gactaataac tccagtaatt gccagtacca atactcctga
     1681 tatggagaca aactcatacc taatccttcc atcaataaac aaaagcaagg taagccctat
     1741 tactccaaaa actatatact gctgaaacat atttttcccc ctaaacaggc cttatagcct
     1801 tataaatcag taactttcat tatagcataa tttttgtccc tgtcatatac aatttaagag
     1861 actcttctta tttttttaca tagttatgga accattttat gcttttttcc atcattcctg
     1921 tagtaacaaa atgatctaga cctggagatt taacaaaact taatttttct ttgcaggtat
     1981 ttagcctatt taacttttcg taaaaaatct cctgactctc tattggtacc attccatcct
     2041 tgtcaccatg taacaaaagg ataggtctat tctttaatag gtccaaatta ttcataggat
     2101 ccatatcttt taccttggct tccaatctct tatctatatc tcctagattc aaagtctttt
     2161 taaatctttc attactatcc tcccaggcac aggaaccatt taatactact agggccttca
     2221 ggccaggttg ccttgtaaaa actccagctg cagtaaagcc tcccatggaa tgacctgtaa
     2281 ctcctatttt ttctggatca gcttcataat catttaccag gctatttatt aaatcagtag
     2341 attcttccag gttttttaac accacatccc agaaaaattt agttaagctt tcttctccat
     2401 agtcactaag tggacttctt tcaccgtgat agatagcatc aggaattaat acctgataac
     2461 ctaggctggc atatata
//
LOCUS       DATGSI010000301         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_444711, whole genome shotgun sequence.
ACCESSION   DATGSI010000301 DATGSI010000000
VERSION     DATGSI010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444711"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VK031_04615"
     CDS             <1..336
                     /locus_tag="VK031_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53232.1"
                     /translation="YLTIHFIRTEDGLDNEYIPLNQSGCMFRGHWGFTNSIKSGRWTP
                     LMQAYRYKKPVRHLQPGGEFDSGYKVVTSKNKLRGSGKAFSLYFETEPEKDCRILGWN
                     ITLTGNAVT"
     gene            336..800
                     /locus_tag="VK031_04620"
     CDS             336..800
                     /locus_tag="VK031_04620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLS53233.1"
                     /translation="MSKKYEVGLLQREDFEWCAEVAAVRMLTEEVGRPELIDIPTIYL
                     IMEKMYQDKSAVVAKVSGEHAGVIGGYLHPSIFNPEYATMAELIWYVLPEYRNTRVGA
                     MVLSEFNKISEESPAHEVTLSLLRTSPINKNTLEKRGFLMEEMAFRKVLKEF"
     gene            802..1362
                     /locus_tag="VK031_04625"
     CDS             802..1362
                     /locus_tag="VK031_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53234.1"
                     /translation="MAAISTIVAVAGLAVAAGGAYMSYRQQREAARNQRRAQEEQRKM
                     NAIQEARQAQEAARERRAQIREERVRRARILQASSNLGVGESSGELGATGALATNLGA
                     GIGHNVGVLDMASRMSAHSQRQADFLSAAAQNQARSSMWGQASSFGSQIFNQAGGFNS
                     IFSGQDVQDVSGKVHQGVRWGRSYSL"
     gene            1372..>2789
                     /locus_tag="VK031_04630"
     CDS             1372..>2789
                     /locus_tag="VK031_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53235.1"
                     /translation="MSSLEDLLGKDEVPLSRLLKQRDQEMTLPEATLRNRAAVSALQA
                     DPDEMVGTYQEMLGEGGEEAYEELSSRLDEATREMDFSTLMDMLADPELSNEVKGDLV
                     VAFQSSPRLTDKTYALADKALMAESEGENIEQEAVRLSVADEIESYMEHKSILQGLTN
                     QFLIDNKKNFLQSTVDFASIIVPGTDAYTNISVTKKIYEALGMEYSTGKTLRDALFPG
                     YSALKVRERFLSLSDQEQIETVSKILDAVKESSGIIAGDDNQVRAAMMLGRFLDGDYT
                     SKDVVLDSLFNLLDIVAIGQLAKTAFRAVRPGSYVKRVKEAEAAQETRGVRKTPEYEG
                     ATVSPPKMSSRAKEIEDLEMEYSRLLSETQSELDPGSVRNLRSELKSIDEELQSLKKA
                     GTRDRAKQPQSERVSPQEARKQAKAEIDDRVKELEGRRQAVTNRLEINARTSTAEQRL
                     GQIEKQLSAARKDTSTVPIRYNS"
BASE COUNT          945 a          486 c          694 g          664 t
ORIGIN      
        1 tatttaacta ttcattttat tcggacagaa gacggactag ataacgagta cataccctta
       61 aaccaatcag gatgtatgtt ccgaggtcat tggggattca ctaattctat aaaatctggc
      121 agatggacac cattgatgca agcatacagg tacaagaaac cagtaagaca tttgcagcca
      181 ggtggagagt tcgatagcgg atataaagta gttacctcaa agaataaatt aagagggtcg
      241 gggaaagcct tctctctgta tttcgaaaca gagccagaga aagattgtag aatattagga
      301 tggaacatta ctttaacagg taatgcagtt acataatgtc taaaaaatat gaagtaggtt
      361 tattgcaacg agaggacttc gaatggtgtg ctgaagtagc tgctgtaaga atgctaactg
      421 aagaagtagg taggccggaa ttgatagata tccctactat ttatctaata atggaaaaga
      481 tgtaccaaga taagagtgct gttgtagcca aggtaagcgg agaacatgca ggagtaattg
      541 gaggatacct acatcctagt atattcaacc cagaatatgc cacaatggca gagcttatat
      601 ggtatgttct cccagaatat aggaacactc gggtaggggc gatggtttta tctgagttta
      661 acaagatatc cgaagaatcc cctgctcacg aagttacttt atctcttctt agaaccagtc
      721 ctatcaataa aaatacttta gaaaagagag ggttcttgat ggaagaaatg gcttttagaa
      781 aagtattaaa ggagttttga tatggcggca atatctacta tagttgctgt tgctggcctt
      841 gctgtagcag ccggtggggc atatatgtct tacaggcaac aaagagaagc agccaggaat
      901 cagagacgag cccaagaaga acaaaggaaa atgaatgcca tccaagaggc tagacaggcc
      961 caggaggcag ctagagaacg acgagctcaa ataagagaag agagggtcag gagggctaga
     1021 atactccagg catcttctaa tttaggggta ggagaaagct ctggagaatt aggggccaca
     1081 ggagccttag ctacaaattt aggggccgga attgggcaca atgttggagt cctagatatg
     1141 gctagcagga tgtctgcaca tagccaaagg caggcagact tcttatcggc agctgctcaa
     1201 aaccaagctc gatcatctat gtggggacag gcttctagtt ttggcagtca aatttttaac
     1261 caggcgggag gatttaattc tatattctcc ggacaagatg tacaagatgt atctggaaag
     1321 gttcatcagg gggtacgatg gggaagaagc tatagcttat aaggatataa tgtgagttct
     1381 ttagaagatt tattaggaaa agatgaggta cctcttagta gattgttaaa acagagggac
     1441 caagaaatga cccttcctga ggccaccctt aggaacaggg ctgctgtttc cgctctacag
     1501 gcagatcctg acgagatggt aggaacatac caagaaatgc ttggagaggg cggagaagag
     1561 gcctatgaag agctctccag taggttggac gaagccacaa gggaaatgga tttctccacc
     1621 ttaatggaca tgttagcaga ccctgaacta tctaacgaag ttaagggaga tttagtagta
     1681 gcgttccaaa gctctccaag gcttactgat aaaacttatg ccttagcgga taaagctctg
     1741 atggcagaga gcgaaggtga gaacatagag caagaggctg ttaggcttag tgttgcagat
     1801 gagatagaat cctatatgga acataaatct attcttcaag ggctaaccaa ccaattccta
     1861 atagataata aaaagaactt cttgcaatct acagtagact ttgcctctat aattgttcca
     1921 gggacagatg cttacactaa catctcagta actaaaaaga tatatgaagc cttagggatg
     1981 gaatactcta caggcaagac tttaagagat gctttgttcc caggatatag tgctttaaaa
     2041 gtaagagaga ggtttttatc tctttctgat caagagcaaa tagaaactgt cagtaaaata
     2101 ctagatgccg ttaaagaaag tagcgggatt atagcgggag acgacaacca agttagagca
     2161 gccatgatgc ttggtaggtt tttagatgga gattatactt ctaaagatgt agtactagat
     2221 tctctattta acctgttaga tatagtggcc atagggcaat tagccaagac agcttttagg
     2281 gcagtaaggc caggatctta tgtaaagagg gtaaaagagg cagaggctgc ccaagaaact
     2341 aggggggtaa gaaagactcc ggaatatgaa ggcgctacgg tatctcctcc taaaatgtct
     2401 agcagggcaa aagaaataga ggatttggaa atggagtatt ccagacttct ttccgaaaca
     2461 cagtccgagt tagacccagg aagtgtaaga aacctaaggt cagaattaaa atctattgac
     2521 gaagagctgc aatctctgaa gaaggcaggg acaagagatc gagccaaaca gccacaatca
     2581 gagagagtgt cgccccaaga ggcccgtaaa caagccaaag cagaaataga tgacagggta
     2641 aaagaattag aaggcagaag acaggcagta actaacagat tagagattaa tgccagaacc
     2701 tctactgcag agcaaaggct tgggcaaata gagaaacaat tatctgcggc cagaaaagat
     2761 acctccactg ttcctattag gtacaattc
//
LOCUS       DATGSI010000302         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_302406, whole genome shotgun sequence.
ACCESSION   DATGSI010000302 DATGSI010000000
VERSION     DATGSI010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302406"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..329)
                     /locus_tag="VK031_04635"
     CDS             complement(24..329)
                     /locus_tag="VK031_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HLS53236.1"
                     /translation="MSGKVFNGFEIVQDLIKLRWVPEILKSIELGNRHYSQILRSIPH
                     LSHTELNRKLNILIKKRVIKRYENDGRIHYELKDFGKDLVHIFDHLEDLEEKYFKIS"
     gene            447..881
                     /locus_tag="VK031_04640"
     CDS             447..881
                     /locus_tag="VK031_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HLS53237.1"
                     /translation="MKNVDLVQKYLSNLAILNVKLHNIHWNVVGKKFIRVHDYTEELY
                     DELFEAFDEVAELLKMKDILPLSTMAEYLENGSIEEVKAKDFTVEESLELVKKDLETM
                     KELATEIRNTADEANDFETVAMFEDYVAFYSKHIWFLNAMLK"
     gene            complement(947..2146)
                     /locus_tag="VK031_04645"
     CDS             complement(947..2146)
                     /locus_tag="VK031_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HLS53238.1"
                     /translation="MYNVRKIFDDLYWVGVNDRRTAMFENIHPIPEGISYNSYLLLDE
                     KTVLLDTVDWSVCDTFIENISHVLDGRDLDYLVINHMEPDHAACIGVVMEKYPNLKVI
                     GNRKTFDFMEQFGYDINSNAVEIKDGDTMSFGKHELMFISAPMVHWPETMATFDKNTG
                     VLFSADAFGTFKSLDGRLYNDEVDFEREFLVPARRYYTNIVGKYGPQVQTLLKKTSDL
                     DIKYICPLHGPVWRNNLDYFIDKYDKWSKYEPEEKGVLIVYGSMYGNTEAAAEDLARR
                     LVEKGMSNISMYDASKTHTSYMIADAFKYSHLVIASATYNLNIYTPVQNFLLDMKALN
                     LQKRTVGIIENGTWAPRAGKLIKDLLGEMRDMHILENDILIKSSMDEGDIDGMEALAN
                     SIIESMK"
BASE COUNT          793 a          478 c          340 g          875 t
ORIGIN      
        1 ttttcggcct tatcccttgt ccactaagaa atcttaaagt atttttcttc taaatcttcc
       61 aaatgatcaa atatgtggac taaatcttta ccaaagtctt ttaactcata atgtatccta
      121 ccatcatttt catatctttt aattaccctt tttttaatta gaatattaag ttttctattt
      181 aattcggtat ggctaagatg gggaatactt cttaaaattt ggctataatg tctatttccc
      241 aattctatag acttaagtat ttcaggtacc caccttaatt ttattaaatc ctgtacaatc
      301 tcaaatccgt taaacacttt tccactcaca ttatcaccgt ctctaacata tttgttcctt
      361 tatgtttata caattatatc atataatgtt attaaagaaa gaaaaagggt aataatacct
      421 atagataaaa aatatggagg gattttatga aaaacgtaga tttagtacaa aagtatttat
      481 ccaatttggc cattttaaat gtgaaacttc acaacatcca ctggaacgtg gtaggtaaaa
      541 aattcatccg tgttcatgat tacacagaag aactttatga tgaattgttt gaagcctttg
      601 atgaagtagc tgaactacta aaaatgaaag atattttgcc actatccaca atggctgagt
      661 atctagaaaa tggtagcatt gaagaagtta aggctaaaga ctttacagtt gaagaatctt
      721 tagagttagt taagaaagac ctagaaacta tgaaagaatt agcaactgaa attaggaata
      781 ctgctgatga agcaaatgac ttcgaaactg tagctatgtt tgaagactat gtagccttct
      841 acagcaagca tatctggttc ttaaatgcta tgttgaagta atttaatagt aaatttaaaa
      901 aaggtataat tggatttttc caattatacc tttttttatt ctatctttac ttcatagact
      961 caataatact attggccagt gcctccattc catctatatc tccctcatcc atggaggatt
     1021 taattaagat atcattttca agtatatgca tatctctcat ctcacctaat agatctttta
     1081 ttaacttgcc tgccctaggt gcccaggttc cattttctat aattcctaca gttctttttt
     1141 gtaggttcaa agctttcata tctaatagga agttttgtac tggagtatat atatttaaat
     1201 tataggttgc agaggcaatc actaagtggc tatatttaaa agcatctgct atcatatagg
     1261 aagtatgggt ttttgatgca tcgtacatgc taatgttact catgcccttt tctactaatc
     1321 tcctagccag gtcctctgct gctgcctctg tgttaccata catggagccg taaacaatta
     1381 gaacaccttt ttcctctggc tcgtatttac tccatttatc atacttatct atgaaatagt
     1441 ctaaattatt tctccatact ggaccatgta gcggacaaat atactttata tctaaatcac
     1501 tggtcttttt cagtaaggtc tgcacctgtg gaccgtattt acctactata tttgtataat
     1561 atcttctagc aggaactaaa aactctcttt caaaatccac ctcatcatta taaagccgtc
     1621 catccagcga tttaaaggtt ccaaatgcat ctgcagaaaa taggactcct gtgttcttat
     1681 caaaggttgc catggtctct ggccagtgca ccattggtgc agaaataaac attaattcat
     1741 gtttaccaaa ggacatggta tctccatctt ttatctccac tgcattagaa ttgatatcat
     1801 agccaaattg ctccatgaaa tcaaaggtct ttctattacc tatgaccttt aaatttggat
     1861 atttttccat tacaactcca atacatgcag catgatctgg ttccatatgg tttatcacta
     1921 gatagtccaa atctctacca tctaaaacat ggctaatatt ttctatgaag gtgtcacata
     1981 ctgaccaatc cactgtatct agcaatacag tcttttcatc taaaagcagg tatgaattat
     2041 aagaaatacc ttctggtatg ggatgaatgt tttcaaacat agcagttcta cgatcgttta
     2101 ccccaaccca atacaaatca tcaaatattt ttcttacgtt gtacattaaa tccccccact
     2161 atacttaata atatataact taagccctaa taggctagtt aacaaagatt tcctgccttt
     2221 ttaaattatt aacaatctac aaataactta tacccagata gaaaaacttt tatctattta
     2281 agataaatta ccataagaaa acccatctcc acttttagaa atgagtcttt cttatggatt
     2341 aacaaatatt taaataggta taggcctgaa aatgtagctt aaggaaagat ttattcctcc
     2401 ttaaattgcc cttcctcatt ttgaccctgt atatctactg tatggtcttc ctcctccaac
     2461 ccctctaagc tttcctcttc cttttg
//
LOCUS       DATGSI010000303         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_350021, whole genome shotgun sequence.
ACCESSION   DATGSI010000303 DATGSI010000000
VERSION     DATGSI010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350021"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="VK031_04650"
     CDS             <1..1308
                     /locus_tag="VK031_04650"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HLS53239.1"
                     /translation="YNKGARIFVVNEDIPLPDDSVKIYVEDSRPILSKISANYFYHPA
                     KELTIIGITGTKGKTTTSNYIREILQSSGINTGIIGTNGIVYNQITETTLNTTPESYE
                     LQRILRSMADSGVSHVVMEVSSGGLMMDRVRDVDFDVAIFTNIYHDHIGPKEHPTFEH
                     YLETKSKLFSMAKHGIINIDAQYSDYIMENSKCSIDTFSIERTSDLQATNIELSKSLE
                     VLGSTFLCNIKGRPNKFQLCMPGIFNIYNALAAIATCLYLEVDIDSIKETLSRVKVKG
                     RVEVLPILDYATVIVDFAHNGVSLQNILTTLKEYKHNRLVCLVGSVGKRSQSRRKEIG
                     DVVAKECDVCILTSDNPDYEDPMEIIDQMARSFVGSNCLLIKEPDRKKAIRQGIQLLE
                     KKDIFLIAGRGHESYHMVNGEKEYFSDQDVAIEAVKDLIHTKK"
     gene            1465..2592
                     /locus_tag="VK031_04655"
     CDS             1465..2592
                     /locus_tag="VK031_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53240.1"
                     /translation="MKRILSLLLVMVLILTLAVGCSKPKDADGDETPDTDQTDAVEDE
                     GTEEPASGNITKVGLGHVASIAKSKDAEEDEAAVAQADLTIAAVGFDEEGKVVSVTID
                     TAQTKVEFDEDLKVASDRAERPKSKKELKEDYGMKSVSEQMGIGKEWYEQIEALEEWM
                     VGKTVEEIKNMKVVERDEAHIAVPDEPDLTSSVTITVESYIDAVAEAWENTVDVENGA
                     TVGLGLITSIGNSKDPEDGNGPVARMDTTMTATAFDEEGVVVGTIIDTAQIKVEYDAE
                     GKVISDKDAEIKTKHELKEDYGMKSVSEQMGIGKEWYEQMNALQEWMVGKTVDEIVNM
                     KVVERDEDHPAIPDEPDLTSSVTITVEAYLEVVELASKAAK"
     gene            2682..>3494
                     /locus_tag="VK031_04660"
     CDS             2682..>3494
                     /locus_tag="VK031_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HLS53241.1"
                     /translation="MKRIIVAILIILVLISVLPGCSKQTKYERYEYSFFDTFDTIIKV
                     IAYTETEEEFNAYMEKLHDRFMELHKLYDKYKNYEGVNNIKTINDNAGKKPVKVEKEI
                     IDLLQFSKEWYCKAGEETNIALGPVIEVWSKYRDKAEADPEKAELPPMNLLEEKAQYT
                     DIDKVIIDEENSTVFLEDENMRIDVGSVAKGYATELVAREIEEAGLKSAIISAGGNVR
                     TIGKPLEKDRKKWGVGIQDPDSLIFDTGSNILDVAFVNDAAVVTSGDYQRYFV"
BASE COUNT         1338 a          431 c          752 g          973 t
ORIGIN      
        1 tataataaag gagctagaat atttgtagta aatgaagata ttccactgcc tgatgatagc
       61 gtcaaaatct atgttgaaga tagtagacct attttatcaa agatttcagc aaattatttt
      121 tatcatccag caaaagagct aacaataatt ggcataactg gaactaaggg taaaacaaca
      181 accagtaact atattaggga aatattgcaa agttcaggta taaatactgg tattatagga
      241 accaatggaa ttgtatataa tcaaataact gaaactacat taaatactac gcctgaaagt
      301 tatgaacttc aaaggatttt aagaagtatg gcagattccg gagttagcca tgtggttatg
      361 gaagtatcgt ctggaggcct aatgatggac agggttaggg atgtggattt tgatgtggca
      421 atatttacaa acatatatca tgaccatata ggtcctaaag aacatcccac atttgaacat
      481 tatttagaaa ctaaatcaaa gcttttttcc atggctaaac atggaattat aaatattgat
      541 gcccaatata gtgactatat aatggagaat tctaaatgta gtattgatac cttttcaata
      601 gaaagaacat cagacttaca ggccacaaat atagagctat ccaagtcttt ggaagtttta
      661 ggctccacat ttttatgcaa tataaaagga agacctaata aattccaatt atgtatgcct
      721 ggcatcttta acatttacaa tgcattggca gcaatagcta cttgcctata tttagaagtt
      781 gacattgaca gcattaagga gacattaagc agggttaagg taaaggggag ggttgaggta
      841 cttccaatac tagattacgc tactgttatt gtggattttg cccataatgg agttagttta
      901 caaaatatat taaccacatt aaaagagtat aagcataata gattagtttg tttggtagga
      961 tctgtaggaa aaaggagtca aagtagaaga aaagaaattg gtgatgtggt ggctaaggaa
     1021 tgtgatgtat gtatattaac atcagacaat cctgactatg aagatcctat ggagataatt
     1081 gaccaaatgg cgagatcatt tgtaggttct aattgtttat taataaaaga acctgatagg
     1141 aaaaaagcta taaggcaggg tattcagtta ttggaaaaga aagatatatt tttgattgca
     1201 ggtagaggcc atgaaagcta tcatatggta aatggagaaa aagaatactt ctctgatcag
     1261 gatgtggcta tagaagctgt taaagatttg attcacacaa agaaatagtg ctgattttct
     1321 taagatagat tctggaataa attaataata tttaataatt tacaaaatag taaaaaaatt
     1381 aacaagcgta ttgtttaatt atagacaata tgctataata gatttgctgt agataaatta
     1441 aattatttag atggagggta ttaaatgaaa agaatattgt cacttttatt agtaatggtt
     1501 ttaattctaa ctctagcagt tggatgttct aaaccaaaag atgctgatgg agacgaaaca
     1561 ccagatacag atcaaacaga cgctgtagaa gatgaaggta cagaagaacc tgcatcaggt
     1621 aacataacga aagttggttt aggtcatgtg gcttcaatag ctaagtctaa ggatgcagaa
     1681 gaggatgaag cagctgttgc acaagctgat ttaacaattg ctgcagtagg atttgatgag
     1741 gaaggtaagg tagttagcgt aactatcgat actgctcaaa ctaaggttga atttgatgaa
     1801 gatttaaagg ttgcttcaga tagagcagaa agaccaaagt ctaagaaaga gctaaaagaa
     1861 gattatggta tgaaaagtgt atctgagcaa atgggtatag gaaaagaatg gtacgagcaa
     1921 attgaggctc tagaagagtg gatggtaggt aaaacagtag aagaaattaa gaatatgaag
     1981 gtagtagaaa gagatgaagc ccacatagca gtaccagatg agccagatct aacatcttca
     2041 gttactataa cagttgaaag ctacatagat gcagtagctg aagcatggga aaatacagta
     2101 gatgttgaaa atggtgctac agttggacta ggacttataa cttcaattgg aaattctaaa
     2161 gatccagaag atggcaatgg accagttgcc cgtatggata ctactatgac agctacagcc
     2221 tttgatgagg aaggtgttgt agtaggaact ataattgata ctgcacaaat aaaagtagag
     2281 tacgatgcag aaggtaaagt aatatctgat aaagatgctg aaattaaaac aaaacatgaa
     2341 ttaaaagaag attatggtat gaaaagtgta tctgagcaaa tgggtatagg aaaagaatgg
     2401 tacgagcaaa tgaatgctct acaagagtgg atggtaggta aaacagttga tgaaattgtt
     2461 aacatgaagg tagtagaaag agatgaagac cacccagcaa taccagatga gccagatcta
     2521 acatcttcag ttactataac ggtagaggct tacctagaag ttgtggaact agcttctaaa
     2581 gcagcaaaat aaattgaata aaaactctcc ctttgggaga gttttttaat ttttaattgt
     2641 tacttatatc tagttttgat atgatgaaat agggtgatta tatgaaaaga attatagtag
     2701 cgattttaat tatattagta ttaatatctg ttttaccagg atgcagcaaa caaacaaaat
     2761 atgaaagata tgaatatagc tttttcgata cctttgacac cataatcaag gtaatagcct
     2821 atactgagac agaagaagag ttcaacgcct atatggaaaa actacatgat agatttatgg
     2881 aacttcataa gttatatgat aaatataaaa actatgaagg agtaaataat ataaaaacaa
     2941 tcaatgataa tgctgggaag aaacctgtca aagtagaaaa agaaattata gatttactcc
     3001 aattttctaa ggaatggtat tgtaaggcag gggaggaaac gaatattgcc ttgggtcctg
     3061 taatagaagt atggtccaaa tatagggata aggcagaagc agacccggag aaggcagagc
     3121 tacctcctat gaatttgtta gaggagaaag cccagtatac agatattgat aaggttataa
     3181 ttgacgagga aaatagcaca gtatttttag aagatgaaaa tatgagaata gacgttggtt
     3241 ctgtagccaa aggttatgct acagaactag tggctagaga aatagaagaa gcagggttaa
     3301 aatctgccat aataagtgca ggtggaaatg ttagaacaat aggaaaacct ctggaaaaag
     3361 atagaaaaaa atggggggta ggtatccaag atcctgatag tttaatattt gatacaggtt
     3421 caaacatact agatgtagcc tttgttaatg atgctgcagt agtaaccagt ggagattacc
     3481 aaagatattt tgtg
//
LOCUS       DATGSI010000304         8620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_302539, whole genome shotgun sequence.
ACCESSION   DATGSI010000304 DATGSI010000000
VERSION     DATGSI010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302539"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VK031_04665"
     CDS             complement(<1..330)
                     /locus_tag="VK031_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53242.1"
                     /translation="MKNFFLVLKFELASFIKNKTFIISTLVICLILAIVVSIPTIKNT
                     FFDSNKEKADKQDIQDGLDLDMGDGRFGFLDETDGAVDKIRLMASFYGGELVEVDSVK
                     KLEEEVKL"
     gene            complement(330..1244)
                     /locus_tag="VK031_04670"
     CDS             complement(330..1244)
                     /locus_tag="VK031_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS53243.1"
                     /translation="MELEVREIYKSFSGKQVLHGVSFKVEPGKALGLLGRNGAGKTTT
                     IRIIMDLFKPNKGEILLNGKKFMPREHQIGYLPEERGLYPKKKVMEQLLYLGQLRGLK
                     KEEARDNANYWLERLEVIEYKDKKLETLSKGNQQKVQLAQTFLCNPNIIILDEPFSGL
                     DPVNSQLLKEIITEFINTEKLIIFSSHQMNYVEEFCDEIALIDEGNIILTGNLKDIKL
                     EYGKNRLTIGSMNLDVDQLERLIKDELGKIVSLVEKRRNYLIVELMAGMTKNNLLQAI
                     VDKEIDLERFSVYEPDLADIFVQKVGEN"
     gene            1410..3470
                     /locus_tag="VK031_04675"
     CDS             1410..3470
                     /locus_tag="VK031_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HLS53244.1"
                     /translation="MKTVFKRFIKNKTKKTKRKGIGINSIKTKLVAYFALLIIVSSMA
                     IGFVSIQHSNKSLIESAEEALSIAVEDGAQLTHSRIELQIRTLEMLAARDDIQNMDWE
                     AQAPVLAREAGRAGFLNMAVTDTSGQAQFIEGNAANLEDRVAVIRALSGESTVSNELV
                     ISRATNDIVLMYTTPIIKDGDIVGALVGDRDGNDLSQLADGTGYGESGYGYVINRNGT
                     FIGHPDRELVLNHFNPINEAENDPKLSSLASFLEEALAERNGVGQYTHDGQELYAAYA
                     PIEGTDWIFVVAADKAEVLASVQTMKKSILTLGLMIAVISLAFVLFVGNSIAAPVIEA
                     VGHSANIANLDISQDLPDKLLKRQDEIGTLSQAFQNITDNLRDIINEINSSSQHLASA
                     SQEMTATTQEAAASSEEVTKVVEEIAKGAAEQALNTEEGASKSNLLGDLIGQEVHVME
                     NLNQATIRTIEAVEKGLVEMESLSNATEKSSQAIQDIYRIILETNDSSNKIGQASELI
                     ASIAEQTNLLALNAAIEAARAGEAGRGFAVVADEIRKLAEQSANSTMDIDNMVKDLQS
                     NADAAVETMERVNQLVAEQTNRLKVSREQYLLIEDTMRAAESVAQESYKLSQQMDEAK
                     DDIIETLQNLTAIAEENSASTEEASASMEEQAASMEQISSSSEELAKLAQNLQAIINR
                     FKLQ"
     gene            complement(3655..5319)
                     /gene="hcp"
                     /locus_tag="VK031_04680"
                     /gene_synonym="priS"
     CDS             complement(3655..5319)
                     /gene="hcp"
                     /locus_tag="VK031_04680"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HLS53245.1"
                     /translation="MKMFCYQCQEASGGKGCTRVGVCGKTADLAALQDLMIYAVKGLA
                     VLGHKADQIGYKKEGLDRFMIEGLFMTITNANFDKERFFEKIKDALILRDELKEELLE
                     KGVDLGQLEDVASFTVNSRDEIEYKANSKEVGVLATEDEDIRSLRELIIYGLKGMAAY
                     AEHADNLGYRDEEIAKFIRRALAATLDDSLTADDLVALAMETGKYGVDVMALLDKANT
                     STYGNPEITEVNIGVRNNPAILISGHDLKDMEELLEQTEGTGVDVYTHGEMLPANYYP
                     AFKKYDHFVGNYGNAWWKQKEEFEKFNGVILFTTNCIVPPKASYADRVYTTGSSGYPG
                     FKHIPDRVDGQPKDFSELIEHAKRLDPPTEIEKGKIVGGFAHAQVFALADKIVEAVKT
                     GAIKKFFVMAGCDGRLRKRNYYTEFAEALPKDTVILTAGCAKYRYNKLNLGDINGIPR
                     VLDAGQCNDSYSLAVIAMKLKEIFELNDINELPIAYNIAWYEQKAVIVLLALLYLGVK
                     NIHLGPTLPAFLSENVAQVLVDKFGIGTISSVEEDIEMFMGEAQEA"
     gene            5541..6443
                     /locus_tag="VK031_04685"
     CDS             5541..6443
                     /locus_tag="VK031_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HLS53246.1"
                     /translation="MKEDNSKICILIVPYTQKVKRILIPKWLPKFSLMALSLILILSS
                     TYLVVNKRQQLKLKNQAKEYVEMVQALEKKNKEKEDRLAQLENQNKVLQAGREDVENK
                     LDEIDALQREVERLTGTKTPSRGGAHSRTIDLDSLEPEEEMEVLCDVLDDKKLELENY
                     VVDLKKQLNYLKTIPDLMPTHGRLTSRFGSRRDPFTGRIRRHTGIDIANSVGTSIRAA
                     GKGQVGFAGLKSGLGRTIIINHGNGYKTVYGHNSRLLVKVGQWVEKGEVIAKMGSTGR
                     STGSHLHFEIHKNNVPINPYDVLK"
     gene            6456..6836
                     /locus_tag="VK031_04690"
     CDS             6456..6836
                     /locus_tag="VK031_04690"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HLS53247.1"
                     /translation="MFRRKDQPTEVEDVDTLIGENIKITGKLEGVGNIRLDGSIDGDI
                     NYEGNIIIGETGRVKGNIVAESISVAGYVKGNIVSDSKLTLLPKASLIGDVEVSSFVI
                     HENARFEGNCKMMANMEEIDREDE"
     gene            complement(6870..7112)
                     /locus_tag="VK031_04695"
     CDS             complement(6870..7112)
                     /locus_tag="VK031_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53248.1"
                     /translation="MHEGCTGSFSNGKEIVDKIRMMGFNTRPMPMEVTIRCVECENDF
                     QMTHLESKCPHCNMVYGVTPCSVAYPERIQAAGIDY"
     gene            complement(7105..8115)
                     /locus_tag="VK031_04700"
     CDS             complement(7105..8115)
                     /locus_tag="VK031_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS53249.1"
                     /translation="MDRKTIGQLIKEYPFLEGYFEENKLTIQGHENKSFRDFVDNLSQ
                     EELEDLALDRVSLITNLQEYIKQMKEFLGLEEDNQVESLTILPGQDKQGQPEGFHKLV
                     IEKSQLVSIVGPTGSGKSRLLADIEWTAQNDTPTGRTILINGKPADKKWRFSSNNRLV
                     AQLSQNMNFVMDLTVKEFLELHAESRLITAVESTVDKIIKEANKLAGEAFDPDTAITS
                     LSGGQSRALMIADTAILSSAPIVLIDEIENAGIDRRKALNLLLDENKIVLMATHDPIL
                     ALMADKRIVIRNGGIAEIISTSKEEKDLLKELEEMDYFIQNMRNKLREGKKLIKGGKK
                     NA"
     gene            complement(8108..>8620)
                     /locus_tag="VK031_04705"
     CDS             complement(8108..>8620)
                     /locus_tag="VK031_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLS53250.1"
                     /translation="CPDHFFASNIEEVVQWGIKEDLDMLITESAGLCNRCSPYIKDIK
                     GICVIDNLSGINTPKKVGPMLRSADIVVITKGDIVSQAEREVFASRVSSVNPRAIVMH
                     INGLTGQGAYELSTLLYDKEEEIKSVEGRELRFPMPAALCSYCLGETRIGKDYQMGNI
                     RKMNLGDNNG"
BASE COUNT         2582 a         1711 c         1565 g         2762 t
ORIGIN      
        1 taactttacc tcttcttcca gcttctttac gctgtcaact tcgaccagct ctccgccata
       61 aaagctggcc ataagtctaa ttttatctac tgctccgtct gtttcatcta aaaaaccaaa
      121 ccttccatcc cccatatcaa gatccaaccc atcttgtatg tcctgcttat ctgctttctc
      181 tttattggaa tcaaaaaagg tatttttaat agtaggtata gaaactacta ttgccaaaat
      241 taagcaaatt actaaagtag agataataaa ggttttattt tttataaagc tggccagttc
      301 aaacttcagt accaaaaaga aatttttcat taattttcac ctaccttttg gacaaatata
      361 tctgccagat caggctcata gacagaaaat ctttccaagt ctatttcttt atctactatg
      421 gcctgaagta gattgttctt agtcatacct gccataagct ctactattag ataatttctt
      481 cttttttcta ccaggcttac tattttgcct agttcgtctt taattaatct ttctaattga
      541 tcaacatcta gattcataga gccaatggtc aatctattct ttccatattc tagttttata
      601 tcttttaagt ttcctgttaa aattatattc ccctcatcaa ttagggcaat ttcatcgcaa
      661 aattcttcca catagttcat ttgatggctg gagaaaataa ttagcttttc tgtatttata
      721 aattctgtaa ttatttcctt taaaagttgt gaatttactg gatctaagcc actaaaaggt
      781 tcatctaaaa ttataatatt aggattacaa agaaaggttt gggctaattg taccttctgc
      841 tggtttccct tggataaggt ttccagcttc ttatccttat attctataac ttctaatctt
      901 tctaaccagt agttggcatt atctctagcc tcttcttttt taagtcccct taactggcct
      961 aggtataata gttgttccat aacctttttc ttagggtaaa gccccctttc ctctggtaga
     1021 tatcctatct gatgctccct tggcataaac tttttaccat ttaaaaggat ttcaccctta
     1081 tttggtttaa aaagatccat tatgatccta atggtagtag ttttaccggc tccgtttctc
     1141 cccagtagac ctaaggcctt acctggttcc accttaaagg aaacaccatg caatacttgt
     1201 tttccagaaa agcttttata tatttctctt acctctagtt ccaaatcctt cacctccgct
     1261 ttctcttttc cccatattaa cataattaga ctttaaatac tattaataag gttgattaat
     1321 tttttttgcc actttaccta aaaagttcat cagtctatcc gatatactaa ctaacagata
     1381 atttaactac ataatattaa ggaggagcaa tgaaaacagt atttaaaaga tttataaaaa
     1441 acaaaactaa aaaaacaaag agaaaaggta ttggtattaa tagtattaaa accaaactag
     1501 tagcgtattt tgccttactg ataattgtat cctcaatggc catagggttt gtatctattc
     1561 agcattccaa taagtcactt attgaatctg ctgaagaagc cttgtccata gcagttgaag
     1621 atggggcaca attaacgcac agtagaatag agttacagat tagaacctta gaaatgttag
     1681 ctgcaagaga tgatattcaa aatatggact gggaagctca agcacctgtc ttggctagag
     1741 aggctggtcg agccggcttc cttaatatgg cagttactga taccagtggc caggcccagt
     1801 ttatagaggg aaatgcagcc aacctggaag atagggtggc agtaattagg gccttatcag
     1861 gggaaagtac tgtatccaat gaactggtaa ttagccgggc caccaatgat atagtactta
     1921 tgtataccac ccccattata aaggatgggg atattgtagg tgccctggta ggtgataggg
     1981 acggtaacga cttaagccaa ctggccgatg ggaccggcta tggagaatct ggctatggct
     2041 atgtaattaa tagaaatgga acgttcatag ggcatcctga tagagagttg gttttaaacc
     2101 attttaaccc tattaatgaa gctgaaaatg atcctaagtt aagttcctta gccagcttct
     2161 tagaagaggc tttagctgaa agaaatggtg taggtcagta tacccatgat ggccaggaac
     2221 tatatgcagc ctatgcacca atagagggta ctgactggat atttgttgtt gctgcagata
     2281 aggcagaggt tttggccagt gtgcaaacta tgaagaaaag tatcttaacc ttaggcctta
     2341 tgattgctgt aatttccctg gcctttgtat tatttgtagg taattctata gcagctccag
     2401 taattgaagc tgtaggccat tctgccaata ttgctaatct ggatatttca caggatttac
     2461 cagataagct tttaaagcgg caggacgaaa taggaacctt aagccaggcc ttccaaaata
     2521 taactgataa tttaagggat ataattaatg aaataaattc atcttcccaa catctggcct
     2581 ctgcctccca agagatgacg gccactaccc aggaagcagc cgcctcatct gaagaggtta
     2641 ccaaggttgt ggaagagata gcaaaagggg cagccgaaca ggccctgaat actgaagaag
     2701 gtgccagtaa gtctaattta ctgggagacc ttattggtca agaagttcat gttatggaga
     2761 atttaaacca ggcaactata agaactatag aggctgtaga aaaaggcttg gtagaaatgg
     2821 aaagcctgtc taatgctacg gaaaaaagta gccaggccat tcaggatatt tatagaataa
     2881 tcttagaaac caatgatagt tccaataaaa taggccaggc cagtgagctt atagcctcca
     2941 tagcagagca aactaatttg ttggcattaa atgcagccat tgaggcagcc agggcagggg
     3001 aagccggtcg aggttttgca gtggtggcag atgaaattag aaaattggca gagcagtctg
     3061 ccaactctac catggatata gataatatgg taaaggattt acaaagtaat gcagatgcag
     3121 cagtagagac tatggaaagg gtcaatcagc tggtggctga acaaaccaat aggctaaagg
     3181 ttagtaggga gcagtatcta ttaattgagg ataccatgag ggcagctgaa agtgtggccc
     3241 aggagtccta taaattaagt cagcagatgg atgaagctaa ggatgatatt atagaaacct
     3301 tacaaaattt aactgctatt gcagaggaaa attcagcttc cactgaggag gcgtcagcct
     3361 ctatggaaga gcaagctgct agtatggagc aaatctccag ctccagtgaa gaattggcaa
     3421 agttggctca aaacctacag gctataatta ataggtttaa gctgcagtaa gtcctataag
     3481 gacttagatt tcaacatgat atgacaatta tgaagaaaag ttaatatcta aggggagctt
     3541 ataatgatag actggaatgg gaatgttatt cctgatcctg aaaagaaccc ttaaaccatt
     3601 tatataggat ataaaataat ccctggtcct tcgaccaggg attatttgtt ttatctatgc
     3661 ctcttgggcc tctcccataa acatttctat atcttcttct acactggaaa tagttccaat
     3721 accaaacttg tctactaata cttgggctac attttctgat aagaaggctg gtagggttgg
     3781 ccctaggtga atatttttca ctcccaggta tagtaaagct agtagtacta ttactgcttt
     3841 ttgctcatac caggcaatat tataggctat tggtagttca tttatatcat ttagttcaaa
     3901 gatttctttt agtttcatgg ctattactgc caaggaatag gagtcattac attggccagc
     3961 atctagcact ctaggtatgc catttatatc tcctaggttt aatttattat acctatattt
     4021 tgcacaacct gctgttaaaa ttacagtatc ctttggtaat gcctctgcaa actctgtata
     4081 gtaatttctt tttcttaatc ttccgtcaca gccagccatt acaaagaact ttttaatggc
     4141 tcccgtcttt accgcctcta ctattttatc agccaaggca aagacttggg catgggcaaa
     4201 gcctccaact atctttcctt tttctatttc tgttggcggg tctagtcttt ttgcatgttc
     4261 aattagttct gaaaaatctt taggctgacc gtctaccctg tcaggaatat gtttaaatcc
     4321 tgggtaacca gaggatcctg tagtgtatac cctatctgca taggaggcct taggtggtac
     4381 tatacagttg gtagtaaaga ggataacacc attaaacttt tcaaactcct ctttttgttt
     4441 ccaccaagca ttgccatagt ttccaacaaa gtgatcatat ttcttaaatg ctggataata
     4501 attggctggt agcatttctc catgggtata tacatcaaca cctgttcctt cagtttgttc
     4561 cagtaactct tccatatcct ttaaatcatg acctgatatt agaatagctg gattattcct
     4621 gactccaata tttacttctg taatctcagg atttccataa gtagaagtgt tagccttatc
     4681 taatagggcc attacatcta caccatattt tccagtttcc atggctaaag ccaccagatc
     4741 atctgctgtt aaactatcat ctaaggtagc agctaaggcc cttcttataa atttagctat
     4801 ttcttcatct ctgtaaccta aattatcagc atgttctgca taagctgcca tgccctttag
     4861 gccatagata attaattctc ttaaggaacg gatatcttca tcttctgtag ccaaaactcc
     4921 tacttcttta gaattggcct tatattcaat ttcatccctt gaatttaccg taaagctggc
     4981 cacatcttcc agttgcccta agtctacacc tttttccaat aattcttcct tcagctcatc
     5041 ccttaggatt agggcatcct tgattttttc aaagaacctt tccttgtcaa agttagcatt
     5101 agttatggtc ataaataaac cttcaatcat gaacctatcc aggccttctt ttttatagcc
     5161 tatttggtct gccttatggc ctaaaacagc cagtcccttt actgcatata tcatcagatc
     5221 ctgtaatgca gccagatctg cagtttttcc acatactcct actcttgtac aacctttacc
     5281 accagaagct tcctggcatt ggtaacaaaa cattttcata tccacattcc ctccatatct
     5341 ataatttatt ccttttttct tactcctatt tattataaat gactgattac ttaagttctg
     5401 taacccttgt taccaaacct atagtttaaa ttttctgatt aattgtggaa ataggcccat
     5461 attattccat atactggcct attatggtat aatacaaagt tagataaatg gcttatctct
     5521 ctagaggaag gatgtgaata atgaaggaag ataattctaa aatatgcatt ttgattgtac
     5581 cctacacaca aaaggttaag agaattttaa tccctaagtg gttgcctaaa tttagcctta
     5641 tggccctttc cctaatttta atcctgtctt ctacatattt ggtagttaat aaaagacagc
     5701 agcttaagct taaaaatcag gctaaggaat acgtagaaat ggtacaagcc ctggaaaaga
     5761 aaaataagga aaaggaagat aggctagccc aattggaaaa tcagaataag gttttacagg
     5821 ccggcagaga agatgtggaa aataagttag atgaaataga tgccttacaa agggaggttg
     5881 aaagacttac tggaacaaaa acaccttcca gaggcggtgc ccatagtaga actatagact
     5941 tagactcctt ggaaccagag gaagaaatgg aagtcctttg tgatgtatta gatgataaga
     6001 agttagaatt ggaaaactat gtggttgatc taaaaaaaca acttaattat cttaagacta
     6061 taccagatct gatgcctacc catggtagat taacttccag atttggtagt aggcgggatc
     6121 ctttcactgg aaggattcga cgtcatacgg gaattgatat tgcaaattct gttggaacaa
     6181 gtattagggc agcaggcaag ggacaggtag gctttgcagg tctaaaaagt ggccttggca
     6241 gaactattat aattaatcat ggtaatggat ataaaacggt ttatggccat aatagtaggc
     6301 ttttggtcaa ggttggccag tgggtagaaa aaggggaagt tatcgcaaaa atgggttcta
     6361 ctggcagatc tacaggatcc cacctacatt ttgaaatcca taagaataat gtccccatta
     6421 atccttatga tgtattaaaa taagggggtg tttaaatgtt taggagaaag gaccaaccaa
     6481 ctgaagtgga agatgtggat accttaattg gagaaaatat aaaaatcaca ggcaagctgg
     6541 aaggtgttgg caatattcgt ctggatggta gcattgatgg agatataaat tatgaaggaa
     6601 atataataat aggggaaact ggcagagtca agggcaatat agttgcagaa tccatttctg
     6661 tagccggcta tgtaaagggc aatattgttt cagattccaa attaaccctg ctaccaaagg
     6721 ctagcctgat aggtgatgtg gaagtttcta gctttgttat tcatgaaaat gccaggtttg
     6781 agggcaattg taagatgatg gctaatatgg aggaaattga tagggaagat gaataaaaaa
     6841 acttgtagtc aaactacaag ttttttcttt tagtaatcta ttcctgctgc ctgtattctt
     6901 tctggatagg ccactgaaca aggcgttacc ccatatacca tattacagtg gggacacttg
     6961 ctctccaggt gggtcatttg aaaatcattt tcacactcta cacatcttat agttacctcc
     7021 ataggcatgg gcctagtatt gaatcccatc atcctaatct tgtctacaat ttcctttcca
     7081 ttggaaaagc taccagtaca accttcatgc atttttcttt cctcctttga tcagtttctt
     7141 tccttctctt aatttatttc tcatattttg aataaaataa tccatttctt ccaactcttt
     7201 taatagatct ttttcttcct tgctggtgct aataatctca gctataccac catttctaat
     7261 aacaatcctc ttatctgcca ttagagccaa tattggatca tgggtggcca taagaactat
     7321 cttgttctca tctaataaaa ggtttagggc ctttctccta tcaataccag cattttctat
     7381 ctcatctata agtacaatag gggctgagct tagtatggct gtatctgcaa tcatcaaggc
     7441 ccttgactga cctccactta gggaggttat ggccgtatct gggtcaaagg cctctccagc
     7501 caatttattg gcctccttaa taatcttgtc tactgttgat tctaccgctg ttatcagcct
     7561 actttctgca tgtagctcta gaaattcctt cactgttaag tccataacaa aattcatatt
     7621 ctgcgaaagc tgggccacca gcctgttgtt agatgaaaag cgccattttt tatcggcagg
     7681 ctttccatta atcaaaatag tccttccagt aggcgtatca ttttgggctg tccattctat
     7741 atctgccaac aacctagact ttccagaacc ggtaggccct actattgaca ccagctggga
     7801 tttttctata actagcttat gaaatccttc aggctgtccc tgtttatcct gtcccggcaa
     7861 gatggttaaa gactctacct gattatcttc ttcaagtcct agaaactctt tcatctgctt
     7921 tatatattcc tgaaggtttg taattaaact aaccctgtct aaggctagat cttccaactc
     7981 ttcctgactt aaattatcta caaagtccct aaaactttta ttttcatggc cttgaatcgt
     8041 aagcttattt tcttcaaagt aaccctctaa aaaaggatac tcttttataa gctggcctat
     8101 ggtttttcta tccattgtta tcacctaggt tcatctttct tatattgccc atttggtagt
     8161 ccttaccaat tctggtttcc cctagacaat aggaacatag ggctgcaggc ataggaaatc
     8221 ttaattccct accttccact gatttgattt cttcttcctt atcatataaa agggtactta
     8281 gttcataggc tccctggcct gttaaaccat ttatgtgcat tactatggct ctaggattta
     8341 cagagctaac cctggatgca aacacttctc tttctgcctg ggatactata tcacctttgg
     8401 taattactac aatatctgct gatcttaaca taggtcccac cttttttggt gtatttatgc
     8461 ctgataaatt atctatgaca catatacctt ttatatcctt tatataaggg gagcacctgt
     8521 tacataggcc ggcagattct gtaatcaaca tatccaaatc ctcttttatc ccccactgga
     8581 ccacctcttc aatattagaa gcaaaaaagt ggtcagggca
//
LOCUS       DATGSI010000305         1132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_252936, whole genome shotgun sequence.
ACCESSION   DATGSI010000305 DATGSI010000000
VERSION     DATGSI010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252936"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VK031_04710"
     CDS             <1..585
                     /locus_tag="VK031_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53251.1"
                     /translation="SIRGYYPSGRVMVAFSYIPEEEPLTFEGVEFKDTFAIIFYKDES
                     GSPVGFVLSGKSLTSESLIEANKLEVKDGGRVGKWDIGKFEGDSYTKLKTQGAFGGLD
                     SPDYVNEIDAELNPFGLLVNRTRYLNDQFPTPTEPNYWIRADISGDSVLDIKSRSNQT
                     ALKINGKIEMGNEEGFTGTINGAEFVNGICVGQA"
     gene            652..>1132
                     /locus_tag="VK031_04715"
     CDS             652..>1132
                     /locus_tag="VK031_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53252.1"
                     /translation="MANIEKPIWSKDKQTLSKVLIRNRLETYNVTDTWYDGTPMDDTK
                     TDDDLVYIKYEGQYLVRNMEEGQVLQKDTMDEMRNLSTFETLLLKMGIYKHVQLNGYY
                     EKGDTPEPINYKISDTSDDDDGGSVIETLNGDKLLHDFNGFYNAEYYGINSSISDNTD
                     "
BASE COUNT          418 a          158 c          251 g          305 t
ORIGIN      
        1 tctataagag ggtactaccc aagtggcagg gttatggttg ccttttctta tatacccgaa
       61 gaagagcctt taacttttga aggagtagag tttaaagata ctttcgccat tatattttac
      121 aaagacgagt ctgggtctcc agtggggttt gtccttagtg gcaagagcct tacttctgaa
      181 agtcttatag aagctaacaa actggaggta aaagacggag gaagggttgg taaatgggat
      241 ataggtaagt tcgaaggaga ttcttataca aaactaaaaa cacaaggagc gtttggagga
      301 ttggattctc ctgactatgt aaacgaaata gatgcagagt taaacccgtt tggattactt
      361 gtaaacagaa caaggtattt gaacgaccag tttcctacac ctactgaacc gaactactgg
      421 ataagggcag atatatcagg agattccgta ttagatatta aatcaagaag taatcagaca
      481 gctctaaaaa taaacggtaa gatagagatg ggtaatgaag aaggatttac aggaactata
      541 aatggagctg agtttgtaaa cggtatttgt gtcggacagg cttagccaat aaaaatgata
      601 aaaagattta tatttgtaat aattaagaaa taaaaagaaa gagactgaag aatggctaat
      661 atagaaaaac ctatttggag taaagataaa caaacgttat ccaaagtact tataagaaac
      721 agattggaga cttataatgt tacagataca tggtatgatg gtactcctat ggacgatact
      781 aagacagatg atgacttagt gtacattaaa tatgaaggac agtacttagt ccgtaatatg
      841 gaagaaggac aagtacttca aaaagatact atggatgaga tgcgtaacct aagcactttt
      901 gagaccttgc ttcttaaaat gggtatttat aagcatgtac agttaaatgg ctactatgag
      961 aaaggggata caccagagcc tattaactac aaaatatcgg acactagtga cgatgatgat
     1021 gggggtagtg taattgaaac cttaaacggg gataaattac ttcatgattt caacggtttt
     1081 tataatgccg aatactacgg aataaatagt agcatatctg acaatacaga cc
//
LOCUS       DATGSI010000306         1338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_252959, whole genome shotgun sequence.
ACCESSION   DATGSI010000306 DATGSI010000000
VERSION     DATGSI010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252959"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1338)
                     /locus_tag="VK031_04720"
     CDS             complement(<1..>1338)
                     /locus_tag="VK031_04720"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HLS53253.1"
                     /translation="SDYFILNLETVLEKNPKPVLEGKEYPNYDNPNRTPKVLRDLGVT
                     AVSLANNHTMDFGPKVLQRTLKRLNKANIKTFGAGKNRKEASNPLKITLKGKKNKKNV
                     YIFTGMRAGKRYQEYSFFAEHNKPGVSSLPQKGLSGQISDLRQKDPESIIIVYPHWQG
                     QDYKWASDNRRIQERCRAFIDAGANYIFGHGTHMLNEIEHYNGGTIAYSIGNFIFNSP
                     GRYKKMQAPPYSLLVNMEIQEQKDGHWTVKNKFFPIITDNKVTKYKVSPINKGKKAYK
                     YMEVKNLEQKQHRKYNLLNELENNDKLTMPIKKVKKFSSIKLLAKEFEAYNIETKVIE
                     GFLVAYINEEPIYFNESRPSTDSQIGTAFAKHKGLTKLLLEEKGISIAKGKIFEREQQ
                     QKALEYTKNFKSAVIKPFAGRKSKGVTVGITTNEEFLAAWNESLNTSKGNILI"
BASE COUNT          354 a          247 c          194 g          543 t
ORIGIN      
        1 tctataagaa tatttccttt agaagtattc agagattcat tccaagctgc aagaaattcc
       61 tcattagttg tgattcctac tgttactccc ttactttttc gaccagcaaa gggctttata
      121 actgcagatt taaagttttt agtgtattct agagcttttt gttgttgttc tctttcaaat
      181 attttaccct ttgcaatgga tatgcctttc tcttccaaca aaagttttgt gagcccttta
      241 tgtttggcaa aagctgtgcc tatctgtgaa tctgttgaag gtctactttc attaaaataa
      301 ataggttctt cattaatata agctactagg aacccttcta tgactttagt ttcaatatta
      361 taagcttcaa attctttggc taataacttt atggatgaaa attttttaac ttttttaatt
      421 ggcatagtca atttgtcatt attttctaac tcattcaaca aattatattt tctgtgctgt
      481 ttctgctcta aatttttaac ttccatatac ttatatgctt tttttccttt attaatgggg
      541 cttaccttat atttagtaac tttattatca gttataatag gaaagaactt atttttcaca
      601 gtccaatgcc catccttttg ctcttgaatt tccatattca ctaacaaact atagggcggt
      661 gcttgcattt ttttgtatct gccaggtgaa ttaaatataa agttgccaat agagtaggct
      721 attgttccac cattataatg ttcaatttca tttagcatat gtgtcccatg accaaatatg
      781 taatttgccc ctgcatctat gaaagccctg catctttctt ggattcttct attatctgaa
      841 gcccatttat agtcctgccc ttgccaatgg ggatacacta taataattga ttcaggatct
      901 ttctgtctaa gatcagatat ttgtccagat agtccctttt gtggcaagga acttactcct
      961 ggcttattat gttctgcaaa aaaactatac tcttggtaac gtttgcctgc cctcatacct
     1021 gtgaatatat agacgttctt tttgtttttc ttccccttaa gtgtaatttt aaggggatta
     1081 gaagcctctt ttctattttt tcccgctcca aaagttttaa tgtttgcctt atttaatctc
     1141 ttaagagttc tttgtaagac tttaggaccg aaatccatag tatgattgtt ggctaaactt
     1201 actgctgtta cccctaaatc ccttaatact tttggagttc tattgggatt gtcatagtta
     1261 gggtattctt ttccttctaa cactggtttt ggattcttct ctaaaactgt ttccaagttt
     1321 aaaataaaat aatcactt
//
LOCUS       DATGSI010000307        18433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_542240, whole genome shotgun sequence.
ACCESSION   DATGSI010000307 DATGSI010000000
VERSION     DATGSI010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 18433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18433
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542240"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..9171
                     /locus_tag="VK031_04725"
     CDS             13..9171
                     /locus_tag="VK031_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLS53254.1"
                     /translation="MDSIVELDANDQIKTEAIIGGSVPATDKNKAILSLRVNPKEQQA
                     PSLKVVAMNGHKTLNVNSLRVVHYDEESFEIEITRDETHWAVCASELRLHTLELEDYE
                     LTAEFVAETWDNYSYEEGDIGVYYPLAYYGAIIRETEWIVEDLRPAHSLPFLLKEGFA
                     QCGWEFSSPIFETDIGKRLWVYVLGEDYGQDNLDAWKAVATGVPGKNTVTGALKKEGD
                     DVYRSMFVGIAEYNFTLDIRDEYFSAGIGNQPAWFDLRIYRQVREEDGSTKNILIEKI
                     GMDYPNKDNSEEHSGEFIRVITWNSTLTTVPGDEIIIQVTYMTAEEVDSRSFTYLIED
                     GSSEPIINNDHIADHKYTLEVTPIRKIPVKGDTLNVADIIDKNLTLMDLLKGTSHLFG
                     RAKLYVNNTNKEIRLYPPFTAKWEDEVIEGFFQYDETFVLDDGIIKDSLEVTGTEITT
                     TRNHTIGYQKWSNPYLKNQDRHVDFDNPKFESGNTDWFNPVFYEVPSRKLVRDTNVRP
                     PMLPEVANNGIEMMYMVDNENDEWTYNIGVVPFIAYGNYYIYGEQVDDEFKYSFFRFE
                     GKWYDTPVSGQKPNFVTLENNEPVEFTPWLIYGEEPLKDLFSIFWRDLFYKESLVNTF
                     SVNVVFNSRQLNKLDYRKLYFIEYAEGFAAWLSKREFNYCTPEGTKLSFIGDVTIEPE
                     SIDEDTIPPVVDPCTKVNAPEIEWQIDEDCILSYDQVGTIESNILNEEWLVRYQGESA
                     FEPAPPSPFNPDKNLYLRYIAHTDDEDCSIPPVTVFINVCSVNVLEAEVTYNENTDCY
                     RVDTVLEGCNTYSDIVFELEIGDNREPLTVSNPSDPVEEWYVEVCEPEDDFCVYITPE
                     LEEGCDIDPTSVCVNLCPKKEDIECVFNEREGGFNFEPKFNIHKPIKKTTLEYDFDSE
                     FKAPTEIDQSDLLEMVGKIYFRVTLEFNDCPDLVVTCSYERSSQDCGLNTPDFDCEIL
                     HDGRLNIIRTGTLVTDAAVDLIRYRGIEQREWQTWDGVPLSACPVYIQRVVIYCNDCP
                     NSCTEIRLCECDISPPPTAAECDSFDVKLLRFSTLLYALPTDFRNDINYIFLKDEVVV
                     QNSSSNIYNGAIEPGSNYIVRALVGACEAQDTFFIESDCIVPEIVYDDYTEYNGEYTI
                     TFETDSEGTIAVTHEFEVDATNTQTVHKTPTISNGVVTLNTTLRPGKNTIKATIENEC
                     GADDAEVTIDVPCPEVNPGLPKLYTICEGCYGNVNLLDLVEGNPDSNGVWVDVDATGV
                     LYGGTADFSQLPVGTYEFMYVVGEGLCALTTTITIVVIPELSAGINTFRQVCHDSGTI
                     NLLDLLSGNPDEGGTWFTEDDIPDLDLDEGTFTPSIDEAEEVYTIAYSHTPKVDKLPE
                     HSECACDSFSVLTIEIEDCTPPPPCPDDSDLGITISEVTNNDPEDDQWDEMLTVSKTG
                     DYENSEIIESDEIYFSYDDGDTWILGDEVKDDDYYEQLRFGFSYGPTSSFDPSPPHYQ
                     WNNIRLTSGDDIDMVRLTSGEGTFNELHTDFTSGNNVRAQGSSFDINRQRSFHLQVRH
                     TLNSGWIIQGHYYLFSRVKVDANGIGGPGPLVDTHVRNKVSGKRKILFERRITYNIDC
                     DPTIIEGSFDPQVPDCPTLPANVGMNITDPQGGFEGHVTCRGTVPGQTASMIWQIEYD
                     VPADAINTRKWSCGNPGPFDGLISLNHEYWMVRIIEMGDYCPPFFIERNYQAPTACNV
                     TGHIICRKDEEARVAWAFRVNNPTVISYDTLQYRINTGPWLSYSNNQNIYVGDEAATV
                     YFRRISNYNNGCPQTTETCNVSFPGVPQPRLSISCSPDNKRININASNLPPGGSYRIY
                     RNGVLFRTQSVAVPVNIDQPGNYYVVYDYTYTSNTIQCGIAPCVPPTLNLQNCTEQDG
                     NIRVNFTSTNLTDTSTLVANITPSAPYDINFNSSTGSGYILVGAHPGQTTDIEVTVDN
                     GCDTATDSITCSMDCISLIIENMQCDYHEGHVTYDIRTSPASSIAPSSNGGYLSNPSH
                     GVYRFRYPSTSGTLTANHPTACETFSRSISCDCVEPNVDITVTCDEETLYIRTVATNM
                     SSLTDEFYYFFIDGIGYAIEHNNNPDFISDIDISGTSHNYTFEYYGCGQKYTQNGSIN
                     CSCADPSIVLSANCSGTTVSYSFDVDNEDNVQGNYTIKNNNQTVGTVSKTGAGTYSGT
                     FTVSAGSNNSVSIEAGNRCGGTVKSNTSSANCSCPPSSLSISNLSCNPNNQEITFNIN
                     SSNVTNKVLKVNGNTVNITSNPQTVQGTTGNNTIEVTAVDSCSNNNLSDTRNVNCQCQ
                     EPSIDLTASCNTSGVISYNFDVTNPSQAQGNFDLLINGTVRDTINNTSASNYSGTYNA
                     SPGSSQSVRIRVNNKCGSTTQSNTVNATCGCPQSSLSISDVICNPNTQMLSFNINSSN
                     VTNRVLRVNGAKHNIGSNPQNIQGQVGNNTITVTGEDSCADKDLADSKQINCACVDTP
                     TVSLGSASLSGSTITVPINTTNISSTSQITFIANGTSRSVTKVSNNSFRASFPAISGN
                     NNYNVSVTNSCGDDTDSGSYSYAPCVPPTITKVNEQFRNDGTFRVTFSTTNCESISAT
                     PVNRLINPPDCNSTYVEFNLLGGSQPFNYSIVAQGCDGLSSQITGTIDPCGNCGISIR
                     SLGCSTVARSADIVFMVDTSNSISTNRYEAIQETIRKTIDHLNDSSGSFRYAVAEYED
                     ASYLTARWITPVRSGNRFTTYNNVAQDIALGTWNRGNHGCAEWQTIWKELNKEFNAGR
                     LNRRPNTVMHIVVLHDSDNGGCSQYGPYSLRNNFLNDHPNTEISVVRFEDTNVNTNVD
                     VASNMMSAAMATVGGSWGVGSNNCAGYPNNCIDTSNTGDPQNSQLPRRMYYFLHPTET
                     TLTDLVEDISSNCRLTVDEAGCPPFHATNFNWTASNGGTILQDDGWFITTNGVGTYTV
                     SATTQHGCTLSSSYSYGLNYTKKVRTKISAPKVSEPVIKKASQYKYDYSKLTTEDNKE
                     LLSLYKDKNYAKIMAFYNKKVESEYFFSCGCDSDEVDKIVKKYLDGLGLL"
     gene            9152..9361
                     /locus_tag="VK031_04730"
     CDS             9152..9361
                     /locus_tag="VK031_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53255.1"
                     /translation="MGWDYSKLPEDKLSYVQQLVQDNNVGALLKVHNNYKLSRYTYCC
                     GTKGILLHFKRLLDERIGGEGKDNK"
     gene            9616..10443
                     /locus_tag="VK031_04735"
     CDS             9616..10443
                     /locus_tag="VK031_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HLS53256.1"
                     /translation="MGIELKLLRSEWAVNLRIGLSYLADIYSLEDRASNLIEESNKVT
                     VHYEGLRSEVYDDVNDISSTSVDGIAVVKLSGAMLLDDGLCHTGIGTFCSRLRALDEL
                     KSIKGILVEVDSGGGESQAGQLLYSTMKDLKKKTGAIVHTAASAAYMGILPADFIMAV
                     GELSLTGSIGALYSIDKEILRMLKEDYLTVYSDLSPQKNIETRELLEDVDIGIKSLLN
                     DTVEIFHDMVLNTRALTYKKKDTLEGGVFRSREAKKRGLIDHIGSYNKALTLMNKII"
     gene            10445..10885
                     /locus_tag="VK031_04740"
     CDS             10445..10885
                     /locus_tag="VK031_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53257.1"
                     /translation="MKWSEVAKRLKTMVGISSDTDIEPEQLITKLENMVQAADTVVTE
                     STDVKGDIESLREQISSVTEVLASHKETMESILQDNKEFKESVESSLQNITSAISSRK
                     EKGIALESLTVQKEDPVEKEPDKNENLKMDINLVHKANRNSWHR"
     gene            10873..12102
                     /locus_tag="VK031_04745"
     CDS             10873..12102
                     /locus_tag="VK031_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53258.1"
                     /translation="MAQIIESAYGQVHIAPGFRDDGTHTGRNLMSFQTEAVINFFRDF
                     TVIKGDIVRNEFGLYSYGSIGRNKKMRFGKFKGNNHLWQKRLNGCTWNPKGSYSHDIT
                     EVVTSPMEAQTEICPDALWESCWEKLMGTGNKKRDLYGTEEVSELMGIILDRVFNDLG
                     DGFYDVAWFGKHPLIERADEEQTYSTSSEEEWMDYKDQQDIHPGFLTYVDHYKYKEGL
                     AHFNVPINNSEVNGPKFVGDPTELFDRLYESATYNFKVANDAGTVYGAPLILVSGSIF
                     QAYEDYLVETHGDLPEMFYYRFNGEFCERLGCIGNQVPGVLRYKGRPVIRMSEWDRWA
                     HMLGVTQHRAMMVPPGIFGLGWDVDSLDQYSGMGLILDQKLSAPDLGKIYMHTMMEMA
                     TALVDTDFITSASIILE"
     gene            12102..12665
                     /locus_tag="VK031_04750"
     CDS             12102..12665
                     /locus_tag="VK031_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53259.1"
                     /translation="MSCTLISLDQSGCIKPFGGLVQSYAVSYDDIEDITFSSPNTVTG
                     ITLDGAATLAKYQYAKDDSAYYNQEGSLNGSINVFEQESMFKFLGINEENQEFVYNVT
                     GCCNLILIHVTNTGTILMQGVEYDDDTEEWRLAKNSTKFSGNINTGTSDEESAVEAIF
                     TSQAFFPSPIVDMTIDALDVLAGESGG"
     gene            12665..12949
                     /locus_tag="VK031_04755"
     CDS             12665..12949
                     /locus_tag="VK031_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53260.1"
                     /translation="MYKYRSNRKEVRFVFPDGDQINLMRAAYTLKLPATKNRPEKRVK
                     VRAATDKDFKRILEDDRYKSIHNKIYEVKDAAAKTNKEKKSDSSDPIPGV"
     gene            12885..14204
                     /locus_tag="VK031_04760"
     CDS             12885..14204
                     /locus_tag="VK031_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53261.1"
                     /translation="MRQLRLIRKRSQIVQTLSQEFDNPILGEIRDYSAVRKTFEQYPI
                     IPYATSSQHTSHKYLDLLLSLVDNNPTLNQCIASIGDYCFNSGQILYKDSKDLFVEEE
                     VLVASPEVSERYTNHLKNIKFYRYDTKVDFNHFIKEMYFERKNGNCYVELVKTNVNGE
                     VNYIYKIHPQRHCLYMLNDDDTLSIVVSKSFDYNYLRKYPAREVPLYPEVDEDDEDIR
                     TMFHFKNRTSGLYGRPDWISALLNVYNAYQDVLYRVKLASKDFTPKVLVEIEGDDPSI
                     DTDLDEDDLLLRIANNFTAGGQDPKTIMVIDRPHGASPAKIHEFQIHTREEYYKAIAE
                     INEREILKANGWSKLLLTGEVKTGFSRAPYEEDLRNREFSVLRKYQTMLSDMFKDLIE
                     PYFLDNDPDMLQYRIDFNLPYKPRDDEDDNDAKTGDRVKPNEKHLLG"
     gene            14149..14775
                     /locus_tag="VK031_04765"
     CDS             14149..14775
                     /locus_tag="VK031_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53262.1"
                     /translation="MTPRQVIELSPTKSTFSDRYIQNIGIIERNVFREHFNYELYENL
                     LDDLAYYLEDEFKLAKTYHEGDTVLYQGSYYTSLVDNNNKLPTENTWKIADKFNDPCY
                     NELWCMGLNRFLAYNIILPAIRYSTYQAGAKGVVMHAEHGDTAEKTVDHRSFEQYLRQ
                     LKSDTESMLDDINHYMDNSDCKDKFFIFDDECGKVKRPRSGLRRILMT"
     gene            14772..15056
                     /locus_tag="VK031_04770"
     CDS             14772..15056
                     /locus_tag="VK031_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53263.1"
                     /translation="MIKKAIKGVLSVKPLDNDQEVKIRYDICKQCEFYDAESDKCTIC
                     KCFMEIKTGLEVNINPIKLRKEVTHCPKGYWGDKEIANLYRERDGLDPLD"
     gene            15303..15764
                     /locus_tag="VK031_04775"
     CDS             15303..15764
                     /locus_tag="VK031_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53264.1"
                     /translation="MKYTRQVVLPYLFREAGLYADGPNTVTISSDGEEILIESEVELE
                     IEGVTASITKCNRQAVCDYCVSMGTVEDPVFSINGEDKEITADWTYGDTDVGAIQTAI
                     ETELDDAIEVIVIDDEVNSLWRVVLSYPCGTGVALNDEEVKVCHTYQDWAE"
     gene            15810..18224
                     /locus_tag="VK031_04780"
     CDS             15810..18224
                     /locus_tag="VK031_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53265.1"
                     /translation="MDNRCAPKVRRDYDTGVLFSYPDKEVFIHRNFIFYTEGQRFFVS
                     DSYGNKFNFLPSHAKLTFDEVLQKLTRCQSPYADVVTDDTLIGKGDEDSPLGVDKDKF
                     IEPGDDVSELNNDAGYLTLDTLPDINDGELDITTGDGLEGSGKFTANQAGDTVIEIAI
                     DKVTIDKINHGETAFGWGDHSVEGYLKADDITGKEDKVNKVTDLDNPNNTTYPTTKAV
                     NDAIQDVDSGIVQSDWKENDSSDRAFIRNKPTKVSDFNIDINTDDISEGNNNLYYTES
                     RVANNSDVSANSAARHTHNNKGALDKVTDSGSGDKYLADDGTYNTVIIPGDDVTNLNL
                     NIDTDDIDEGSNNKYYTENRVEGVIDNKVTKNYIDNLGIDAVTLNGKDNYILDTEKGS
                     PGGVATLNSSGKLSTSQVPAIALTETFEVNDINERDALPTGTGEGAVGIGDVVIVSDA
                     SDDPEVDEGGATYIYTGSEYKRLVTPTDDVISVNGQNGIVVLDTDDISEGSTNKYFTN
                     ARVSNNSDVSANTNARHTHSNKSALDNIINSGNGKNALMNDGTYKSVVRPGDDVTDLN
                     LNITSDNVPEGNSNRYYTNDRVSNNTDVSANTSARHTHSNKSALDKIISTGSGNKYLS
                     DDGTYKIKEDPEWGKITGDIEDQADLKNAFDNKSDKGHTHTSSEITDFQSAVTNNTDV
                     ENATTHIGRTDNPHNITAAQVGVDKNFIDGLGVDAATLNGKSDTDFATSAQGNKADTA
                     LQPGDDVSELLNNVGYVTEGGTLGLAIHGDDEDTQRPSGFSSVVWIGEVEPENANEND
                     IWVNDV"
     gene            18217..>18433
                     /locus_tag="VK031_04785"
     CDS             18217..>18433
                     /locus_tag="VK031_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53266.1"
                     /translation="MFKDVNIHIKKDGLWKPVYEDGFWYLHFPAGGPSGITSNRVEAR
                     KTSDFEIGSYPSDIEIGIRLLDEKTAYV"
BASE COUNT         6122 a         2962 c         3935 g         5414 t
ORIGIN      
        1 acttttgatc aggtggactc aattgtagag ttagatgcta atgatcagat taagactgaa
       61 gctattatag ggggttcagt tcctgctacc gataagaata aagcgattct atccttacgt
      121 gtaaacccta aagaacagca agcaccatca ttaaaagtag tggcaatgaa tgggcataag
      181 acactgaatg taaactcttt aagagttgtc cactacgatg aggaatcttt tgaaatagag
      241 ataactagag acgagacaca ctgggctgta tgtgcttcag aacttaggtt acatacttta
      301 gaacttgaag attatgaatt aactgctgag ttcgtagctg aaacttggga taattactcg
      361 tatgaagagg gtgatatagg tgtatattac cctttagctt actatggagc gataattagg
      421 gaaactgagt ggattgtaga agatctacgg ccagctcact ctttgccttt cttacttaaa
      481 gagggatttg ctcaatgtgg ttgggaattt tcttctccta tattcgaaac agatataggt
      541 aagaggttat gggtgtacgt tttaggtgaa gattatggtc aagataatct agatgcatgg
      601 aaagctgtag ccactggtgt accaggtaaa aacaccgtta ctggtgcctt aaaaaaagaa
      661 ggtgatgatg tctatagatc tatgtttgta ggtatagcag aatataactt caccttagat
      721 atacgtgatg aatactttag tgctggtata ggtaaccaac cggcttggtt cgatttacgt
      781 atatacagac aagttagaga agaggatgga agcactaaaa acatacttat agaaaagata
      841 ggtatggatt accctaataa agacaactca gaagagcata gtggtgaatt tattagagtt
      901 ataacttgga actctacact taccactgta ccaggtgatg agataattat acaagttacc
      961 tatatgactg ctgaagaagt ggacagcagg tcttttacct accttataga ggatgggtcc
     1021 tctgaaccta ttataaataa tgaccatatt gctgatcata aatatacttt agaggttaca
     1081 cctatacgta agatccctgt aaaaggtgat acccttaatg ttgctgacat tatagataag
     1141 aaccttactc ttatggactt attaaagggt acttcgcact tatttggtag agctaagctg
     1201 tatgtaaata acacgaataa agagataaga ttatacccac catttacagc taagtgggag
     1261 gatgaggtca ttgaaggatt ctttcagtat gatgagacat ttgtattaga tgacggtatt
     1321 ataaaggatt ctttagaagt tactggtaca gagataacta ctactagaaa ccatactata
     1381 gggtaccaaa aatggagtaa cccgtactta aagaatcaag ataggcatgt cgattttgat
     1441 aaccctaagt tcgagtccgg caatactgat tggtttaacc ctgtatttta tgaagttcca
     1501 agcagaaaac tggtaagaga tactaatgta cgccctccga tgctacctga agtagctaat
     1561 aacggcatcg aaatgatgta catggtagat aatgagaatg atgaatggac gtacaatatc
     1621 ggtgtagtgc ccttcatagc ttacggtaac tactatatat atggtgagca ggtagatgat
     1681 gagtttaaat actctttctt cagatttgaa ggtaaatggt acgatacccc ggtatctggg
     1741 cagaaaccta actttgttac tttagagaat aacgagcctg tagaatttac accttggttg
     1801 atttatgggg aagagccttt aaaagatttg ttttctatat tttggaggga cctattctat
     1861 aaagagtcac tagtaaacac atttagtgta aatgtagtat tcaattcaag gcagttaaat
     1921 aagctagatt acagaaaact atattttata gagtatgctg aaggttttgc agcgtggttg
     1981 agtaagagag aatttaatta ttgcacccct gaagggacta aactatcttt tataggtgat
     2041 gtaactatcg aacctgaaag catagatgaa gatactatac ccccggttgt agatccgtgt
     2101 actaaggtca atgcgcctga gatagaatgg caaattgatg aagattgtat attatcttat
     2161 gatcaagtag gcactataga aagtaatata ttgaatgaag aatggttggt tcgttatcaa
     2221 ggtgaatcag catttgagcc tgcccctccc tctcctttta accctgataa aaacctatat
     2281 ttaaggtata tagcacatac agacgatgaa gattgctcta tacctccggt tacagtgttt
     2341 attaacgtgt gttcagtaaa tgttttagag gctgaagtaa cgtataacga aaacacagat
     2401 tgttataggg tggacacggt gctggagggt tgtaacactt attcggatat agtttttgaa
     2461 ttagagatag gcgataatag agaaccatta actgtatcta acccttcaga tcccgtagag
     2521 gaatggtatg ttgaagtatg tgaacctgag gatgactttt gcgtgtatat caccccggag
     2581 ttagaggagg gttgtgatat agatcctact tctgtttgcg ttaatctatg ccctaaaaaa
     2641 gaagatatag aatgcgtatt taatgagcgt gagggcgggt ttaattttga acctaaattc
     2701 aacatacaca aacctataaa gaaaactact cttgagtatg actttgattc agaatttaag
     2761 gcacctacag aaatagatca atctgattta ctagaaatgg tgggtaagat ttactttagg
     2821 gttaccctag agtttaatga ttgccctgat ctagtggtta cctgtagcta cgaaaggagt
     2881 tcacaagatt gcggtttaaa tacaccagat tttgattgtg agattcttca cgatgggagg
     2941 ctgaatatca ttagaaccgg cacattggtt acagatgcgg ccgtagattt gataaggtat
     3001 agaggaattg agcagagaga atggcagaca tgggatggag tacctttatc tgcttgccct
     3061 gtttacatac agagggttgt aatttactgt aatgattgcc ctaatagttg tacagagata
     3121 aggttatgtg agtgtgacat ttctccacct cctacagcgg cagagtgtga ttcttttgat
     3181 gttaaattat tgagattcag tactttactg tatgcgttac caacagattt taggaatgat
     3241 attaattaca tattcctaaa ggatgaagta gtggtccaga actcttctag taatatttat
     3301 aatggtgcta tagaacctgg gagtaactac atagtaaggg ctttagtagg ggcgtgcgag
     3361 gctcaggata cattctttat agagagtgat tgtatagtac ccgaaatagt ttacgatgat
     3421 tatactgaat acaacggtga gtacactata actttcgaaa ctgatagtga aggtactatt
     3481 gctgttacgc atgaatttga agttgatgca actaataccc aaacagtaca caaaacacct
     3541 actatatcta atggtgtagt aactttaaac acaactttaa ggcctggaaa aaatacaata
     3601 aaagcgacta tagagaatga gtgtggtgca gatgatgctg aagttactat agatgtacct
     3661 tgtcctgagg tgaatccagg cttacctaaa ttatacacaa tttgtgaagg gtgctatggt
     3721 aatgtaaacc tcctagattt agttgaaggt aaccctgatt ctaatggggt gtgggtggat
     3781 gttgatgcta ctggtgtact ttatgggggt actgctgact ttagtcagct tcctgtaggt
     3841 acttatgagt ttatgtacgt agtaggtgaa ggtttgtgtg cattgactac tactattact
     3901 atagtagtta ttccagagct atcagcaggt ataaatacct ttagacaggt atgccatgat
     3961 tctggaacta ttaatttact agatttactt tcaggtaacc ctgatgaagg gggtacatgg
     4021 tttacggaag atgatatacc tgatctagac ttagatgaag gtacctttac accctctata
     4081 gacgaagctg aagaggttta tactatcgct tactcacata cccctaaggt agataagcta
     4141 cctgaacata gtgaatgtgc ttgtgattct ttttcagtat taactataga gatagaggat
     4201 tgtacccctc ctcctccttg tccagatgat tcagatttag gtattacaat atcagaagtg
     4261 actaacaatg atcctgaaga tgatcaatgg gatgagatgt taaccgtttc taagaccgga
     4321 gactatgaaa attctgagat tatagaatca gatgaaattt acttttctta tgatgatgga
     4381 gatacttgga tactagggga tgaggtgaag gacgatgact actacgaaca gctaaggttt
     4441 gggttttcct atggccctac cagctccttc gatccctccc cccctcacta tcagtggaat
     4501 aacattagat tgacttctgg agatgatatc gatatggtta ggttaacctc tggggagggt
     4561 acgtttaacg agttacatac agattttact tcgggtaaca atgtgcgtgc gcaaggatct
     4621 tcgttcgata ttaataggca acgttcattt catctgcaag ttagacacac tttaaattcc
     4681 ggatggataa tacaagggca ttactatcta tttagtagag tcaaggtaga tgctaatgga
     4741 attgggggcc ccggtccttt ggtagataca catgtaagga ataaagtgtc agggaaacgg
     4801 aaaatattgt ttgaaaggag gattacttac aatattgatt gtgaccccac tattatagaa
     4861 gggtcttttg atcctcaagt tccagattgt ccaacattac ccgccaatgt gggtatgaac
     4921 attacagacc ctcagggggg ttttgagggg catgtaactt gtagggggac agtcccggga
     4981 cagactgcca gcatgatttg gcaaatagag tacgatgtac ctgctgatgc tataaataca
     5041 cgaaagtggt cttgtggtaa tcctggtcct tttgatggct taatatccct aaaccacgaa
     5101 tattggatgg taaggattat agagatggga gattattgtc cgccattctt catagaaagg
     5161 aattatcagg ctcctacagc ttgtaatgtc actgggcata ttatctgtag gaaagacgaa
     5221 gaagctagag tagcttgggc atttagggtg aataatccaa cggtaattag ctacgatact
     5281 ttgcaataca ggataaatac tgggccttgg ttatcttata gcaacaatca aaatatttat
     5341 gttggagacg aagccgcaac agtgtatttc cgcagaatat ctaattataa taatggttgc
     5401 cctcaaacca ctgaaacttg taatgtatct ttccctggag tacctcagcc tcggctaagt
     5461 atttcatgta gccccgataa taaaagaata aatattaatg ctagtaatct acctcctggg
     5521 ggtagttata ggatatacag aaacggggtc ttatttagaa cccagtcagt agctgtaccc
     5581 gttaatatcg atcagccggg taattactat gtagtgtatg actatacata cacttctaat
     5641 actatacagt gtggtatagc tccttgcgtt cccccaactt taaatttgca gaactgtact
     5701 gagcaggatg gtaatataag agtcaatttc acatccacta acctgacaga cacatctaca
     5761 ctagttgcta atataacccc tagtgctcct tacgatatta acttcaatag tagcacaggt
     5821 agtggttaca tactggtagg agctcaccca ggtcaaacaa cggatatcga agtcactgta
     5881 gataatgggt gcgatactgc tacagactct attacatgtt ctatggattg catctcgtta
     5941 attatagaga atatgcaatg cgattaccat gaaggtcatg taacttacga tattagaaca
     6001 agcccagcta gttctatagc tcctagctct aatggtggtt atttatctaa cccttctcat
     6061 ggtgtatata gatttaggta cccttctaca tcaggcactc tgacagctaa ccaccctact
     6121 gcttgtgaaa cttttagtag atctatttct tgtgattgtg tagaacctaa tgtagatatc
     6181 acagtaacgt gcgatgaaga gaccttgtat ataaggactg tagctactaa catgagtagt
     6241 cttacagatg aattttacta tttttttata gatggtatcg gttacgctat agagcataat
     6301 aataatcctg actttatatc agacattgat atatcaggga catcacataa ctatacattt
     6361 gagtattatg gatgcggtca aaaatacact caaaatggat cgattaattg tagttgcgct
     6421 gatccttcta ttgttttaag tgctaattgt tcaggaacta cagtatctta tagttttgat
     6481 gtagataatg aagataatgt acaaggtaat tacactataa aaaataacaa tcagactgtt
     6541 ggtacagtaa gtaaaacagg tgctggaact tactcaggaa cgtttacggt tagtgctgga
     6601 tctaataatt cggtatctat agaagctggt aaccgatgcg gtggaactgt aaagtctaac
     6661 acatcttctg ctaattgctc ttgtccaccg tcaagtttaa gtataagtaa ccttagttgt
     6721 aaccctaata atcaagagat tacattcaat ataaactcta gcaatgttac taataaagta
     6781 cttaaagtta atgggaatac agtaaatatt acctccaatc ctcaaacagt tcaaggaact
     6841 acaggaaata atactattga agtgactgcg gtagattctt gtagtaataa taatttgagc
     6901 gatactagaa atgtgaattg ccaatgtcaa gagccttcta tagatttaac ggctagttgt
     6961 aatacttcag gggttatttc gtataatttt gatgtgacta accctagtca agcacaaggt
     7021 aatttcgatt tattaataaa tggtacggta agagatacta taaataacac tagtgcatct
     7081 aattactctg ggacatataa tgctagccct ggtagttctc aatcagttag gattagagtt
     7141 aataataaat gtggatcaac tactcaatct aatactgtaa atgctacttg cgggtgcccc
     7201 caatcctctt taagtatttc tgatgttata tgtaacccta acactcagat gttatcattc
     7261 aatataaact ctagcaatgt tactaataga gtgcttagag taaacggcgc taaacacaat
     7321 ataggttcta atcctcagaa tatacaaggt caagtgggga ataataccat aacagttaca
     7381 ggagaggata gctgcgcaga taaggatcta gcagattcta aacagataaa ttgcgcttgt
     7441 gtagataccc ctacagtatc tttagggtcg gcatctttat cggggtcaac tattactgta
     7501 cctataaata caactaatat atcttccact agtcagataa cttttatagc taatggcaca
     7561 agtaggtcag taactaaagt aagtaataat tcttttaggg cttcgttccc agctattagt
     7621 ggtaataaca actacaatgt aagtgtaact aatagttgcg gtgatgatac tgattcaggt
     7681 agttattctt acgctccatg tgttccacca actataacta aagttaatga acagttcagg
     7741 aatgatggga cgtttagagt aactttctct actactaact gcgaaagtat atcagccacc
     7801 cccgttaata ggttgataaa ccctcctgat tgtaactcga cctatgtaga gtttaatcta
     7861 ctaggtggta gccaaccttt caactactca atagtagctc agggttgtga cggtttaagt
     7921 agccaaatta caggaactat agatccttgt ggtaattgcg gtatatctat acgtagttta
     7981 ggttgctcta cagtagcccg tagtgctgat atagttttta tggtagatac ttcaaactca
     8041 ataagtacaa ataggtacga agctatacaa gaaacaatta gaaaaactat agaccaccta
     8101 aatgactctt caggatcttt taggtatgcc gtagcggagt atgaagatgc aagctactta
     8161 actgctagat ggataacccc tgttaggtca ggcaatcgat ttaccactta taataatgta
     8221 gctcaggata ttgctctagg tacatggaat agaggcaacc atggttgtgc agaatggcag
     8281 actatttgga aagagcttaa taaagagttt aatgcaggta ggttaaatag acgacctaat
     8341 acagttatgc atatagtagt gcttcacgat tctgataatg gtggatgctc tcaatacggg
     8401 ccctatagct tacgcaataa cttcttaaat gaccatccaa atactgaaat aagtgtcgta
     8461 aggtttgagg ataccaatgt aaacactaat gtagacgttg cttctaacat gatgtcagct
     8521 gccatggcta ctgtaggtgg gtcgtgggga gtaggtagta ataattgtgc aggataccct
     8581 aataactgta tagacaccag taatactggc gaccctcaaa atagccagtt acctagaaga
     8641 atgtactact ttttacaccc tacagaaaca acattaactg atctggtaga agatatatcc
     8701 tctaactgta gattgactgt agatgaggcc ggttgccccc ctttccatgc tacaaacttt
     8761 aattggacag cttctaacgg tggtaccata cttcaggatg acggctggtt tataaccact
     8821 aatggggtag gcacttacac tgttagcgct acaacccaac atggatgtac tttgtccagt
     8881 agttatagtt atggattaaa ttacactaaa aaagtacgga ctaagatatc agcccctaag
     8941 gtatcggaac cagtaataaa aaaagcttca caatataagt atgattattc caaacttaca
     9001 actgaagata ataaagaatt gctatcttta tataaggata aaaattacgc aaagataatg
     9061 gcgttctaca acaaaaaagt agagtcagaa tacttcttta gttgtggttg cgattctgat
     9121 gaagtagata aaatagtaaa aaaatattta gatgggttgg gactactcta aattaccaga
     9181 agataaatta tcttatgtac aacagttggt acaagataat aatgtaggcg cactgttaaa
     9241 ggtacacaac aactataaat tatctaggta cacttactgt tgcggcacta aaggaatatt
     9301 actacacttt aaaagattac ttgatgaaag aatcggcgga gaaggaaaag ataataaata
     9361 gattaataaa gagaggggta gtagatgaag gggataatac tagaaaattc cttaaagact
     9421 tgaggaaagt cactctatgc gctcctttag ttaagttaga tagagaaact aagaaattct
     9481 cactcagaca actttgtgta aggtataacc taactattat gcaagtaaga aacatattaa
     9541 aaaagtgata taatatatca cagttaatcc aattatattt catagttatg tttatttcct
     9601 ttagttttac agactatggg aatcgaatta aaattactca gatcagaatg ggctgtaaat
     9661 cttaggatag gtttatcgta cctagctgac atatactcac tggaagatag agcatccaat
     9721 ctgatagagg aaagtaataa ggtaactgta cattacgagg gtttacgatc agaggtttat
     9781 gatgatgtaa atgatatatc ctcaactagt gtcgacggta tcgccgtagt taagctatct
     9841 ggtgctatgt tacttgatga tggtttatgt cacacaggta ttggtacttt ttgtagtagg
     9901 cttagggcac tagatgaact taaatctatc aaaggtattt tagtagaggt agattctgga
     9961 ggtggtgaat ctcaggctgg tcagttgctg tatagcacta tgaaagattt gaaaaagaaa
    10021 acaggtgcta ttgtacatac agcggcctca gcagcttata tgggtatctt accagctgat
    10081 tttataatgg ctgttgggga attatccctt accgggtcta taggtgcatt gtactctata
    10141 gataaagaaa tactaagaat gttgaaagag gattacctta ctgtttatag tgatttaagt
    10201 cctcaaaaga atatcgaaac aagggagcta ttagaggatg tggatatcgg tataaagtca
    10261 ttactgaatg atactgttga gattttccat gatatggttc taaatactag agctctcaca
    10321 tacaaaaaga aagatacttt agaaggtggc gtatttaggt ctagagaagc taagaagaga
    10381 ggtcttatag atcatattgg gtcatacaac aaagcattaa ctttaatgaa taaaattatt
    10441 taatatgaaa tggtcagaag tagctaaaag gctaaaaact atggtaggca tcagttccga
    10501 taccgacata gaacctgaac aacttataac taagttggag aatatggtac aagctgctga
    10561 tactgttgta actgaatcta ctgatgttaa aggagatata gagtctctta gagagcaaat
    10621 tagtagcgtt actgaagtat tagctagcca taaggaaact atggaatcta tcttacagga
    10681 taataaggag ttcaaagaat cagtagagag ttctctacaa aatataacta gtgctataag
    10741 ctctaggaaa gagaaaggta tagcattaga atcattaaca gtacagaaag aagatccggt
    10801 agagaaggag cctgataaaa acgaaaatct taaaatggat attaatttag ttcacaaagc
    10861 aaacagaaac tcatggcaca gataattgaa tcagcatatg gtcaggttca tatagcacct
    10921 ggttttagag atgacggtac acatacaggc agaaatctga tgtcctttca aaccgaagct
    10981 gtaatcaact tctttagaga ctttacagta attaaaggag atattgtacg taacgaattt
    11041 gggctgtatt cttatgggtc tattggccgt aataagaaga tgcggttcgg taaatttaaa
    11101 ggtaataacc atttatggca gaagcggctt aacggatgta cgtggaaccc taaaggaagc
    11161 tactctcatg atatcactga agtagttact tcgcctatgg aagctcagac tgagatatgt
    11221 cctgatgctt tatgggagtc ttgctgggaa aaacttatgg gtaccggaaa taaaaagaga
    11281 gatctgtacg gtacagaaga agtttctgaa ttgatgggta ttatattaga tcgggtattt
    11341 aacgacctag gtgatgggtt ttacgatgtt gcctggtttg gtaagcaccc tctgattgag
    11401 cgtgcggacg aagagcagac ttactctact tccagtgaag aagagtggat ggactacaaa
    11461 gatcagcaag atattcaccc aggattcttg acttacgtcg accattataa gtataaagaa
    11521 gggttagctc actttaatgt ccctatcaac aatagcgagg tcaatggacc taaatttgta
    11581 ggtgacccta ctgagttgtt tgatcgattg tatgagagcg ctacctataa ctttaaagta
    11641 gctaatgacg caggtactgt ttatggagca ccattgatcc tagtatcggg atctattttc
    11701 caagcttatg aagattatct agtagaaact cacggtgatt tgcctgaaat gttctactac
    11761 cgctttaatg gggagttctg cgaacgttta gggtgtatcg gtaatcaggt accgggagta
    11821 cttagatata aaggccgacc agttattcgt atgtctgagt gggatcgttg ggcacatatg
    11881 ttgggagtta ctcaacatag agcaatgatg gtaccaccag gaattttcgg cttaggatgg
    11941 gatgtagatt cccttgacca atattccggt atgggcctga tccttgatca gaaattatca
    12001 gcaccagact taggtaaaat ctacatgcat acgatgatgg agatggctac agcgttggta
    12061 gacactgact ttattacttc agcatctata atcttagaat aatgagttgc acactaataa
    12121 gccttgatca gtcaggatgc atcaaaccgt ttggcggttt ggtgcaatcc tatgctgtat
    12181 cgtacgatga tatagaagac attaccttct cttcacctaa tacagtaaca ggtattactt
    12241 tagatggtgc ggctacctta gctaaatacc agtacgctaa agatgactct gcttactaca
    12301 atcaagaagg tagtttaaac ggtagtatta atgtttttga acaagaatca atgttcaagt
    12361 ttttgggtat caatgaagag aaccaagaat ttgtctacaa cgttacaggc tgctgtaatt
    12421 taattcttat tcatgtaaca aataccggta ctatcttgat gcagggtgta gagtatgatg
    12481 atgatacaga ggagtggcgc ttagccaaga atagtactaa attttctggt aatattaata
    12541 ccggtacatc agatgaagag agtgctgtag aagctatctt tacatctcaa gcattcttcc
    12601 cgtcccctat agtagatatg actatagatg ctttagatgt tttagccggt gaaagtggag
    12661 gataatgtat aaatacagaa gtaatagaaa ggaggtaaga tttgtattcc ctgatgggga
    12721 ccagataaat cttatgcggg cggcatacac tttgaagctg cccgcaacta aaaaccgccc
    12781 tgaaaaacgg gtgaaagtaa gggctgctac agataaagat tttaaacgta tcttagagga
    12841 cgatagatac aaatcaattc ataataaaat ctatgaagta aaagatgcgg cagctaagac
    12901 taataaggaa aagaagtcag atagttcaga ccctatccca ggagtttgat aaccctattt
    12961 taggtgagat aagagattac agtgctgtta ggaaaacttt cgaacagtac cctataatac
    13021 cttacgctac aagtagtcaa catacatctc ataagtattt agatctatta ttatctttag
    13081 tagataataa tcctacacta aaccagtgta ttgctagtat aggtgattac tgcttcaatt
    13141 caggtcagat cctatataaa gattcgaagg atttattcgt agaagaagaa gtactagtag
    13201 cttcaccgga agtgagtgag agatatacca accacctaaa aaatataaag ttttacaggt
    13261 acgatactaa agtagacttc aaccatttca taaaggaaat gtactttgaa cgtaagaatg
    13321 gtaactgtta tgtcgaactt gtaaaaacta atgtgaatgg ggaggttaac tacatttata
    13381 agatacatcc tcagagacat tgtctttata tgctaaacga cgatgacaca ttatctatag
    13441 tagttagtaa gagttttgac tataactacc ttagaaaata ccccgctaga gaagttcctt
    13501 tatatcctga agtagatgaa gatgatgagg atataaggac aatgttccat tttaaaaaca
    13561 ggactagtgg gctatatggt agacctgact ggatatcagc cctacttaat gtttataatg
    13621 cttaccagga cgttctatac agggtaaaat tagctagtaa agactttaca cctaaagtat
    13681 tagtagagat agagggggat gacccctcta tagatactga tctagatgag gatgacttac
    13741 tacttcgtat agctaataat tttacagctg gtggacaaga tcctaaaact attatggtga
    13801 ttgaccggcc tcatggagct tcaccagcca agatacatga gtttcagata catactagag
    13861 aagagtatta taaagcaatt gcagaaataa atgaaaggga gatattaaaa gctaatggat
    13921 ggtctaagtt acttttaact ggcgaagtaa aaaccggttt tagtagagct ccctatgaag
    13981 aagatctaag aaatagagag ttctcagtat taaggaagta tcagactatg ttgtcagaca
    14041 tgttcaaaga cttgatagag ccttatttct tagataacga tccagatatg ttacagtata
    14101 ggatagattt taatttacca tataaaccta gagatgatga ggacgataat gacgccaaga
    14161 caggtgatag agttaagccc aacgaaaagc accttctcgg atagatacat acaaaatata
    14221 ggtatcattg aaaggaatgt tttcagggag cattttaatt atgaattata cgaaaatctt
    14281 ttagatgact tagcttatta tctagaagat gaatttaagc ttgctaaaac ttatcacgag
    14341 ggtgatactg ttctttatca aggtagctac tatacttcgc ttgtagataa taataataag
    14401 ttacctactg aaaatacgtg gaagatagct gataaattta atgatccttg ctataacgag
    14461 ttatggtgta tgggtttaaa taggttttta gcttataaca ttatacttcc agctattagg
    14521 tactctacat accaggcagg ggctaaggga gtggttatgc acgctgaaca tggggatacc
    14581 gctgagaaaa cggttgacca tagatctttc gaacagtatt taagacaatt aaaatcagac
    14641 acagaatcta tgttagatga tatcaatcat tatatggata atagtgattg taaagataaa
    14701 ttctttatat ttgatgatga gtgtggtaaa gttaaaagac cacgatcagg attaaggaga
    14761 atattaatga catgattaaa aaggctatta aaggtgtgct tagtgtaaaa cctctagata
    14821 atgatcaaga ggtaaagata aggtatgata tttgtaaaca gtgtgaattt tatgatgctg
    14881 aatctgataa atgcactatt tgcaaatgtt ttatggagat aaaaacaggg ttagaagtaa
    14941 atataaatcc tattaaactt aggaaagaag ttactcattg ccctaaagga tattggggag
    15001 ataaggaaat cgccaatcta tacagagaaa gggatggcct agacccatta gattaatatt
    15061 atttatcaat gttacgatta cttgcaaatc cagaacagaa tacaaaaaat ttacttagag
    15121 tagttgagtc caaaaccgga tgctgcggtt ctgaaaaaac cgaatgtcag tacacttaca
    15181 acgatgattc actagttgaa gtaactaaaa tcgcttacgg tgacaaggaa gtcaatttag
    15241 gtgataagca ggagggtata gatattgaga gggggtacga aggtaaagac acctctgaaa
    15301 aagtgaagta cactcgtcaa gttgtgctgc cttatctatt ccgggaggct ggtttatatg
    15361 ctgatggtcc caacacggtt actatcagtt cagatggtga agagattctg attgagtctg
    15421 aagtagagct tgagattgaa ggagttacag ctagtataac taaatgtaac agacaagcgg
    15481 tatgcgatta ttgcgttagt atgggaactg ttgaggatcc agtattctct atcaatggtg
    15541 aagataagga aattaccgca gactggactt acggagatac tgacgttgga gctatccaaa
    15601 cagctattga gactgagtta gatgatgcta ttgaagttat tgttatagac gatgaagtaa
    15661 atagcctttg gagagttgta ttgtcttacc cttgcggtac aggggtagct cttaatgatg
    15721 aagaagttaa agtatgccat acttatcaag actgggcaga ataatttatg gcacttttag
    15781 aaataaaatg cgtttccggc aggcttcgga tggataaccg ttgtgccccc aaagttcgta
    15841 gagactacga cacaggggta ctattttctt atccagacaa agaagtattt atccatcgta
    15901 attttatttt ttataccgag ggtcagagat tttttgtctc tgactcttac ggtaataagt
    15961 ttaacttctt accctcccat gctaaactga cttttgatga agttcttcaa aaacttacta
    16021 ggtgtcaatc accttacgct gatgttgtta ccgatgacac attaataggt aaaggtgacg
    16081 aagattcacc tttaggtgta gataaagata aatttatcga acctggtgac gatgtatctg
    16141 aattaaacaa tgatgcaggt tacctgactt tagacacttt accagatatc aatgacggtg
    16201 agttagatat aactacagga gatgggttag aggggtcagg taaatttaca gctaatcagg
    16261 ctggtgacac tgttatagag atagctattg ataaagttac catagataag ataaaccatg
    16321 gtgaaaccgc tttcggatgg ggtgatcatt cagtagaggg gtatcttaaa gcagacgata
    16381 tcacaggtaa agaagataag gttaacaagg taacagacct agataaccct aacaatacta
    16441 cctaccctac aacaaaagct gttaacgatg ctatacagga tgtagattca ggtatagtcc
    16501 aaagtgattg gaaagaaaat gatagctcgg atagagcttt tataaggaat aaacccacaa
    16561 aagtatctga cttcaatatt gacatcaata ctgatgatat ttcagagggt aataataatt
    16621 tatattacac agaatctaga gtagctaata attccgatgt ttcagccaac tcagctgcaa
    16681 gacatactca caacaataag ggagctttag ataaggtaac cgattccggc tccggtgata
    16741 agtacttagc cgatgatggt acctacaata ctgttattat accgggagac gatgttacga
    16801 accttaatct taatatagat acagatgata ttgatgaggg ctccaataat aaatactata
    16861 ctgaaaatag agtagaaggg gttatagata acaaggttac taagaattat atagataatt
    16921 taggtataga cgctgtaaca cttaatggta aagataacta catcttagac actgaaaaag
    16981 ggagtcctgg aggggtggct accctaaata gtagcggtaa attatctact tcccaggttc
    17041 cggccatcgc tcttacagaa acttttgaag taaatgatat taatgagaga gatgccttac
    17101 ctactggtac aggagaaggg gctgtaggta taggtgatgt tgttattgtt tcagatgctt
    17161 cagatgatcc tgaagtagat gagggaggag ctacctatat ttacaccggt tcagaataca
    17221 aaagacttgt tacccctact gacgatgtaa taagtgttaa cggtcagaat ggtattgtag
    17281 tactagacac cgatgatata tcagagggtt caactaataa atacttcaca aatgctagag
    17341 tatctaataa ttctgatgta tctgctaata ctaatgctag acacacacac agtaacaaaa
    17401 gtgctttaga taatataata aattcaggta acggcaagaa tgctctaatg aatgacggta
    17461 cgtacaagtc tgtagtacgt cccggggatg atgtcactga tcttaatcta aatatcacct
    17521 cagataatgt tcctgaagga aatagtaata ggtattacac aaatgatagg gtatctaata
    17581 acacggatgt ttcagcaaac acgtcagcac ggcacaccca cagtaacaaa agtgctttag
    17641 ataaaataat aagtacgggt tcaggtaaca agtatttatc cgacgatggt acatataaga
    17701 taaaggaaga tcctgagtgg ggtaaaataa ccggcgacat tgaagatcaa gcggatctta
    17761 aaaatgcttt tgataacaaa tcagataaag gtcacactca cacatcttca gaaataactg
    17821 attttcaaag tgctgtaaca aataatacag atgttgagaa tgctactaca catataggta
    17881 gaaccgataa tccccataac atcacggctg cacaggttgg tgtagataag aatttcatag
    17941 atgggttggg ggttgatgct gcaaccttaa acggtaaatc agataccgat tttgctactt
    18001 ctgctcaagg gaataaagca gatacagctt tacagcctgg tgacgatgta tcagaattgt
    18061 tgaataacgt aggttatgta actgagggcg gtactttagg tttagctatc catggggatg
    18121 atgaagatac tcagagacct tccgggttca gctctgtagt ttggataggt gaagtggagc
    18181 ctgaaaatgc taatgaaaac gacatttggg taaacgatgt ttaaggatgt caacatacat
    18241 ataaaaaaag atggcttatg gaaaccggtg tatgaggatg ggttctggta tttgcatttt
    18301 ccggcaggag gacctagtgg gataacaagc aacagggtag aggcaagaaa aacatcagat
    18361 tttgaaatcg gatcctatcc ttcagacata gaaataggaa taagactgct cgacgaaaaa
    18421 actgcttacg tgc
//
LOCUS       DATGSI010000308         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_117700, whole genome shotgun sequence.
ACCESSION   DATGSI010000308 DATGSI010000000
VERSION     DATGSI010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117700"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /gene="cbiQ"
                     /locus_tag="VK031_04790"
     CDS             <1..679
                     /gene="cbiQ"
                     /locus_tag="VK031_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HLS53267.1"
                     /translation="MIDRYAYTNKLSKYNPNHKGLIAITALALTIGLNNNYINLLVFL
                     VMAILTTFIAKIPVKQYLKILAIPKGFLLISIISIVLSLSKDNNFIFSVKIYDYYLGI
                     SQENLNTSINLFTRSLGAISGSLFLGLTTPLNQLIKLLKKLKLPNIVVELIVLIYRFI
                     FMFLEEAKQIYIAQKIRFGYAGRKNSYKSLSLLIKELFLRLILGYKQMQTSLECKLYR
                     GEFKIGD"
     gene            680..1504
                     /locus_tag="VK031_04795"
     CDS             680..1504
                     /locus_tag="VK031_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS53268.1"
                     /translation="MLKINSLEYKYEDGTRALKGINMDLNRGNIIGLIGANGSGKSTL
                     FLNILGILRPSKGKIEYKGKPIKYKKKDLINYRKEVNLVFQNPEKQIFYSNIRDDIAF
                     SLRNLNYDEREVERRVVKALKKVKALDLIERPAHFLSYGQKKRIAIASILVLDTKVLL
                     LDEPTAGLDPEMTREMMEIIKSLTKDRKILIASHDMDFIYQICDYIYLLKEGEILGEG
                     RAEEVFLESKLLEEAGLDRPWLVKVHKRLGLPLYKTESEFEKNWKGDRNEKGNNSF"
     gene            1479..2261
                     /locus_tag="VK031_04800"
     CDS             1479..2261
                     /locus_tag="VK031_04800"
                     /inference="COORDINATES: protein motif:HMM:NF017944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HLS53269.1"
                     /translation="MKKGIIVSSFGTTYKETRDLCIGSIENLIKEEFEGDLVLRAFTS
                     EIVRRILKERDKLTIDNVPSALERLKEKGISNIYIQPLHIIAGIEYEKILRQVERFKE
                     RGENKDFSIKIGSPLLSGESSYERVIEALSLPEDKPGRGLVYMGHGSSHEADQSYENL
                     ERAIRKKGFENVFIGTIEGSKGIDCILKELKEKQIRQVDLKPFLLVAGDHANNDMASD
                     DKDSWKSILERNNIEVTTELKGLGQVKEIQNIFLNKLRELYQ"
     gene            2261..>3050
                     /locus_tag="VK031_04805"
     CDS             2261..>3050
                     /locus_tag="VK031_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLS53270.1"
                     /translation="MKALMITGPNSNSGKTIISLGLIRALKKLGYNISPFKTGPDFVD
                     TKYLSQAANRPAGNLDLYLMGEEGIRQALAMDRGDLALVEGAMGYFDGIHNSYENSSY
                     HISKELDIPAILVYSPKGEMFSAVTKIKGMVDFKGSKIQGVILNKVSKGMYQLLKEQI
                     ERHVGVKVLGYLPWIEEAEIKSEILGLELSKKEELLDTLADKLSQTIFLKDLIKLAQD
                     TEPASLNYPRKRDLKIALAYDKAFCFYYRENIRLLEEICQVKYFS"
BASE COUNT         1113 a          438 c          662 g          837 t
ORIGIN      
        1 aatgatagat aggtatgcct ataccaacaa gctatctaag tataatccta atcacaaggg
       61 tctaatagcc ataactgcct tggccctgac tataggactt aataacaact atataaatct
      121 tttagtattt ttagttatgg ctatactaac tacatttata gcaaaaattc cagtaaagca
      181 atatctaaaa atcctggcta tacctaaagg attcttactt ataagtataa tttccatagt
      241 cctttccctg tctaaggaca ataactttat atttagtgtt aagatatatg attattacct
      301 gggaataagc caggaaaact taaataccag tataaactta tttaccagat ccttaggagc
      361 catatctggt tccttgtttt tagggcttac tacccctcta aatcagctta taaaactact
      421 taaaaaacta aaactaccaa atatagtagt ggagctaata gttttaatat ataggtttat
      481 ctttatgttt ttagaggagg caaagcaaat ttatattgca cagaaaatta gatttggtta
      541 tgcaggaagg aaaaattctt acaaatcctt aagccttcta atcaaggagc tttttctaag
      601 gttaatccta ggatataaac agatgcaaac ctccttggaa tgtaagctgt ataggggaga
      661 atttaaaata ggtgattaaa tgttaaagat aaattcttta gagtacaaat atgaggatgg
      721 gaccagggcc ttaaaaggaa taaacatgga tctgaatagg ggaaatatta taggcctaat
      781 tggggccaat ggatctggaa aatcaactct ttttctaaat atcctaggaa ttttaaggcc
      841 tagtaaggga aaaattgaat acaagggaaa gcccatcaaa tataagaaaa aagacttaat
      901 taattatagg aaggaagtga acctggtttt tcaaaatcct gaaaaacaaa ttttctattc
      961 caatataaga gacgatatag cctttagttt aaggaattta aactatgatg aaagagaagt
     1021 agaaagaagg gtagtgaagg ccttaaaaaa ggtgaaagct ttggacttaa tagaaaggcc
     1081 tgcccatttc ctttcctatg gacaaaagaa aagaatagcc atagccagca tattggtatt
     1141 ggatactaag gttttgctct tagatgagcc tacagccggc ttggatcctg aaatgaccag
     1201 ggaaatgatg gagataatta aaagcttgac caaagataga aaaatattaa tagccagcca
     1261 cgatatggac tttatctacc agatctgtga ttacatttac cttttaaaag aaggagaaat
     1321 tctgggagag ggaagggcag aagaggtttt tcttgagagc aagttattgg aagaggcagg
     1381 gttggacagg ccttggctgg taaaggtaca taaaagatta ggtctaccct tatataagac
     1441 tgaaagtgaa tttgaaaaga attggaaagg agacagaaat gaaaaaggga ataatagttt
     1501 ctagtttcgg aaccacctat aaggaaacaa gagatttatg cattggaagt attgaaaact
     1561 tgattaagga ggagtttgaa ggagacctgg tattaagggc ctttacatct gaaattgtaa
     1621 gaaggatact gaaagaaagg gataagttga ccatagacaa tgtacccagt gccttggaaa
     1681 ggttgaaaga aaagggaatt tccaatatat atatacagcc tttacatatt atagctggga
     1741 ttgaatatga aaagatctta aggcaggtag aaaggtttaa ggaaagaggg gaaaacaaag
     1801 atttttccat taagatcggc agccccctac tgtccggtga gagctcttat gaaagggtaa
     1861 tagaagcctt gtccctgcca gaagataagc ctggaagggg actggtatat atgggacatg
     1921 gtagctccca tgaagctgac caatcctatg aaaatttgga aagggccatt aggaagaaag
     1981 gattcgaaaa tgtatttata ggaactatag aaggaagcaa agggatagat tgtattttaa
     2041 aggaacttaa ggaaaaacaa ataaggcaag tagacctaaa gccatttcta ttggtagcag
     2101 gagaccatgc caacaatgat atggcatctg atgataagga ctcttggaag agtatcttag
     2161 aaaggaacaa tatagaagta actacagaat taaagggatt aggccaggtt aaggagatac
     2221 aaaatatatt tttaaataag ttaagggagc tttatcaata atgaaggcct taatgattac
     2281 cggccccaat agtaattcgg gaaaaaccat aatcagctta ggcctaataa gggcccttaa
     2341 gaagctggga tataatatat caccctttaa aacaggacct gactttgtag atactaaata
     2401 cttatcccag gctgccaata ggccggcagg taatctagat ctttatttaa tgggagaaga
     2461 aggaatcagg caagccttgg ccatggacag gggagactta gccctggtgg aaggggccat
     2521 gggttatttt gacggcatac ataatagcta tgaaaactct agttatcata taagtaagga
     2581 gttagatata cctgccattc tggtttatag cccaaaggga gaaatgtttt ctgccgtaac
     2641 taaaatcaag ggtatggttg attttaaagg ttcaaagatc cagggtgtta ttttaaataa
     2701 ggttagcaag ggcatgtatc agcttttaaa ggagcaaata gaaaggcatg taggcgtaaa
     2761 ggtattaggg tatttacctt ggattgaaga ggcagagata aaaagtgaaa tcttaggact
     2821 agaattatct aagaaagagg aacttttaga taccttggcg gataagctta gccaaacaat
     2881 atttcttaag gacttaataa agctggctca agatactgag ccagccagtt taaactatcc
     2941 taggaaaagg gatttaaaaa ttgctctggc ctatgacaag gccttttgct tctattacag
     3001 ggaaaatata aggttgctgg aagagatttg ccaggttaaa tatttttctc
//
LOCUS       DATGSI010000309         5688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_302870, whole genome shotgun sequence.
ACCESSION   DATGSI010000309 DATGSI010000000
VERSION     DATGSI010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302870"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VK031_04810"
     CDS             <1..701
                     /locus_tag="VK031_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLS53271.1"
                     /translation="HDLGHTPFGHTGERVLNELHPRGFKHNIQSLRVVDFLEHSTKRI
                     GLNLTYEVREGIKNHSGNNKSETLEGQIVKISDRIAYINHDIDDAIRAGIISSEDLPR
                     DCIEVLGKSHGGRINRMILDIIETSLDTNNISMSKEVGEKTEKLRQFMFDHVYINSKA
                     KSEEEKSEHIIRELYNYYLNNLEKLPAEYRKEINGYDASIEDRICDYIAGMTDRYAVN
                     TYKNIFIPKPWEKY"
     gene            788..2605
                     /gene="dnaG"
                     /locus_tag="VK031_04815"
     CDS             788..2605
                     /gene="dnaG"
                     /locus_tag="VK031_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HLS53272.1"
                     /translation="MTIYIDNETIEKIKDSGDIVDIVSDYVQLKRSGANFVGLCPFHN
                     EKTPSFTVSQSKQFFHCFGCGESGDLITFVMKRENLDFTEAIKFLGERLGIPIESKEV
                     DKKYINEREEAYRINREAARFFYKNLTTNNNALKYLKKRQISAKTIRQFGLGFAQDNW
                     ESLYNYLTKRGFKAEEIEKIGLIAKGSGNNSYYDRFRNRIIFPIIDTRSRVVGFGGRV
                     LDDTMPKYLNSRDSIVFNKGRHLYGLNLVSKFSDREKILLVEGYMDVISLYSRGITYS
                     VASLGTALTEEQGKLLRRYGKEVYICFDSDTAGIKASFKAIDVLLKIGVSPRIVLLPD
                     NMDPDDYINKYGLFEFEKLINQSLHYIDYKIHVNKKKYDLNRPEDTIKFTKEIVKVIK
                     SLKSPVEQDVFIKKISQDTGISKEAIEREIREGSSTYRRTDRKRKINKDRPIIKPVQD
                     RISSGSKKAQLDLIRLAIDNREYFEVINESLTNYNIFNQNYQKIFQLIENEYEDKNSL
                     DKDALFDTLLRQDIDKKLVEAIFHGKLEYEPTDMEQVLSDLINTIILERLEEARKDII
                     KQIEDIERKKSSDQIDDGLKKLFMRLTDINERIKLLRQE"
     gene            2619..3737
                     /gene="rpoD"
                     /locus_tag="VK031_04820"
     CDS             2619..3737
                     /gene="rpoD"
                     /locus_tag="VK031_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HLS53273.1"
                     /translation="MGRKSKNTNEIENKQEIVKELIAKGKKSGVLTYKEIADALSEID
                     LSSDEMDDIYKRFEDMGIEITGDRDEEDLLDTDAELDDKDDEKDDDLSVPKGIMVDDP
                     VRMYLKEIGKIPLLTGEEEVELAKRMEAGDEMATKQLAEANLRLVVSIAKRYVGRGML
                     FLDLIQEGNLGLMKAVEKFEYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVE
                     TINKLVRVQRQLVQELGRDPTPEEIGKEMDMDVDRVREIMKIAQEPVSLETPIGEEED
                     SHLGDFIPDEDVIAPADAATFTMLREQLIDVLDTLTPREQKVLRLRFGLDDGRARTLE
                     EVGREFDVTRERIRQIEAKALRKLRHPSRSKKLKDFLE"
     gene            3794..4489
                     /locus_tag="VK031_04825"
     CDS             3794..4489
                     /locus_tag="VK031_04825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLS53274.1"
                     /translation="MNLSNRLKEIIKLVPKNSIVADIGTDHGQIPVYLIENKISKRVI
                     ATDISKPSLDKTIDLIKERKLEDKILTKLGDGLEVLRPFQVDTVIMAGMGGILITEIL
                     ESNRKVTDSISHFIFQPMVASKELRQYLVENQFKIVDESLAREGERFYEIILAKKGPS
                     TIEKDIYYELGPKLIEKNHPLLRTYIEKKIFQLNNIKNSLKKAKDAEALEKKRDIIEK
                     IKAYREVLETIES"
     gene            4479..5579
                     /locus_tag="VK031_04830"
     CDS             4479..5579
                     /locus_tag="VK031_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HLS53275.1"
                     /translation="MKAKRIIQAMDKWVPSKLIDSWDNTGFQIGDEEREVKKVLVALD
                     LDRPTLKKAIEENYQMVITHHPLIFKPLNRINSKDYTGRLIMDIIKNDIVVYNAHSNL
                     DMAIDGVNDELANIFNLKDSKILRKVYHDELVKLAVYTPKDYADQIRFALGNSGAGHI
                     GNYSHCTFNSDGIGTFLPTADAKPYIGKVGTLEKVEEVKVESILKREDLSHILAEVKK
                     AHPYEEVAYDVYPLLNEGTAYGYGRVGYIEETEAEEFIYQVKDKLKVSDLTAYGNIDG
                     TVSKVALCGGSGSNFIYDAHKAKADIYITGDLKYHDAQLAQELGMILLDAGHFHTEKI
                     ILPVIKKYLLEKLGEELSIKVLMESSLPEKTY"
BASE COUNT         2220 a          721 c         1220 g         1527 t
ORIGIN      
        1 gacatgattt aggacatacc ccttttggac atactggaga acgggtgcta aatgagttac
       61 atccaagagg ttttaaacac aacatccaaa gcctaagggt ggtagatttt ttggaacata
      121 gtaccaaaag aatagggtta aacctaacct acgaggtcag ggaaggcatc aaaaatcatt
      181 ctggcaataa taaaagtgag actttagaag ggcaaatagt aaaaatttct gacagaatcg
      241 cctatattaa ccatgacata gatgatgcca taagggctgg tattataagt agtgaagatt
      301 tacctaggga ctgtatagaa gtgttaggaa aaagccatgg tggccgaata aataggatga
      361 ttttggatat tattgagaca agtttagata ctaataatat aagcatgagc aaggaggtag
      421 gagaaaagac agaaaaatta aggcagttta tgtttgatca tgtttatatt aatagtaagg
      481 ctaaatctga ggaggaaaag tccgaacata taattaggga attatataat tattatttaa
      541 ataatttaga aaagctacct gctgaatata gaaaagagat taatggctat gatgcctcca
      601 tagaggatag gatatgcgat tatatagcag gtatgacaga caggtatgct gttaatacat
      661 acaaaaatat atttattcca aaaccctggg aaaaatatta ggaaaaaaga aggatttcct
      721 ccatttatgt cgaatagtag ttaagtgggc atctttatag gatgccggtg aatatttagg
      781 tgattatatg acaatttata tagataatga aacaattgaa aaaataaagg atagtggtga
      841 tatagttgat attgtttcag attatgtcca attaaaaaga tcaggagcca attttgttgg
      901 cctatgtccc tttcataatg aaaagacacc ttcttttacg gtctcccaat caaaacagtt
      961 tttccattgc ttcggttgtg gtgaaagtgg agatttaata acctttgtta tgaaaaggga
     1021 aaatttggac tttacagaag ctattaaatt cctaggcgaa agactgggaa ttcctataga
     1081 aagtaaagaa gttgataaaa aatatataaa tgaaagggag gaggcctata ggataaatcg
     1141 agaagcagca agattctttt ataaaaattt aactactaat aataatgcct taaaatattt
     1201 aaagaaaaga cagataagtg ctaaaaccat cagacagttt ggattaggtt ttgcacaaga
     1261 taattgggaa agtttataca attatttaac aaaaaggggg tttaaggcag aggaaataga
     1321 gaaaatagga ttgattgcta aaggaagtgg gaataatagc tattatgata ggtttcgcaa
     1381 cagaatcatc ttccctatta tagataccag atccagggtt gtaggatttg gaggaagggt
     1441 tttagatgat actatgccaa aatatttaaa ctctagagat agtattgtct ttaataaggg
     1501 acgccattta tatggcctaa atcttgtaag caagttttca gatagggaaa agattttgtt
     1561 ggtagaaggc tatatggatg ttatttcctt atattctagg ggtatcacat attccgtagc
     1621 cagtttggga actgctttaa ctgaggaaca ggggaaactt cttagaagat atggcaagga
     1681 agtttatata tgtttcgact ctgatacagc aggaatcaaa gcaagcttta aagccataga
     1741 tgtgctctta aaaataggag taagcccaag gatagtactg ctaccggata atatggaccc
     1801 agatgattat ataaataagt atggtttatt tgaatttgaa aagctaatta atcaatcctt
     1861 gcattatata gattataaga ttcatgtaaa caagaaaaaa tatgatttaa ataggcctga
     1921 agatactatt aaatttacta aggaaatagt taaggttata aaaagcctta aaagtccagt
     1981 ggaacaagat gtatttataa agaaaatttc acaggataca ggcatatcaa aagaggccat
     2041 agaaagagag ataagagagg gttcttctac ctatagaagg actgatagaa aaagaaagat
     2101 aaataaagat aggcctataa tcaagcctgt tcaagacagg atatcttcag gatctaaaaa
     2161 agcacaactt gatttaataa gattggcaat agacaatagg gaatattttg aagtgataaa
     2221 tgaaagtttg actaactata atatttttaa ccaaaactat cagaaaatat tccagctgat
     2281 tgaaaatgaa tacgaagata aaaatagctt agataaggat gcactttttg atactctttt
     2341 aagacaggat atagataaaa aactagtaga ggccatattc catggaaaat tagaatatga
     2401 gcctaccgat atggaacaag tattatctga tttaattaat actattatat tagagaggct
     2461 ggaagaagcg agaaaagaca ttattaaaca aatagaagat atagaaagga agaaaagctc
     2521 tgatcaaata gatgatggtt taaaaaaatt gtttatgaga ttaacagata taaatgaaag
     2581 gataaaattg ctaagacaag agtaaggagg tatcacaatt ggggcgaaag tctaaaaata
     2641 caaatgagat tgaaaacaag caagaaatag ttaaggaact aattgcaaag ggaaaaaagt
     2701 ctggggtatt aacatataaa gaaatagctg atgcattaag tgaaatagac ctatccagcg
     2761 atgaaatgga tgatatctat aaaagatttg aagatatggg aatagaaata acaggggata
     2821 gggatgaaga agatctactt gatactgatg ctgagctaga tgacaaggat gatgagaaag
     2881 acgatgatct aagtgtacct aaaggtatta tggttgatga ccctgtaagg atgtatctaa
     2941 aagaaatagg aaaaatacct ttattaacag gcgaggaaga agtagagctg gccaaaagaa
     3001 tggaagcagg ggacgaaatg gccaccaaac aattggctga ggccaactta agattggtag
     3061 taagtattgc caaaagatat gtaggaagag gtatgctatt tttagacctg attcaagagg
     3121 gaaatctagg tctaatgaag gctgtagaaa aatttgaata caggaaagga tttaaattta
     3181 gtacctatgc cacttggtgg attagacaag cgataaccag ggccatagca gatcaggcta
     3241 gaacaatcag aattccagtc cacatggtag aaactataaa caaattagtt agggtacaga
     3301 gacaattggt tcaggaatta ggaagagatc caacaccaga ggaaataggt aaagaaatgg
     3361 atatggatgt tgacagggtt agagaaatta tgaagatagc ccaggaacca gtgtccttag
     3421 aaacacctat aggggaagag gaagacagcc acctgggaga ctttattcca gatgaagatg
     3481 taattgctcc tgcagatgct gcaaccttta ctatgctaag agagcaacta atagatgtgt
     3541 tagatacctt aacacctaga gagcagaagg tactaaggtt aaggtttggc ctagatgatg
     3601 gtagagcaag gaccttggaa gaagtaggaa gagaatttga tgtaaccagg gaaagaatca
     3661 ggcaaataga agcaaaagcg ctaaggaagc taagacaccc tagcagaagt aaaaaattga
     3721 aagattttct agaataatta aagattcgct tctttgcgaa tctttaatct tattcttttt
     3781 aaggagaata tatatgaatt taagcaatag attaaaggaa ataataaaat tggtgccaaa
     3841 aaattctatt gttgctgata taggaacgga tcatggccaa attcctgtat atttaataga
     3901 aaataagatt agcaaacggg taatagctac agatattagc aagccttctt tagataaaac
     3961 tatagattta attaaggaaa gaaaactaga agataaaatc ttaacaaagc tgggagatgg
     4021 cttggaagtt ttaaggcctt tccaggtaga tacagtaatt atggcaggga tgggcggaat
     4081 actaataaca gaaatattag aaagcaatag aaaggttaca gactccataa gccactttat
     4141 ctttcaacct atggtagcct ctaaagaatt aagacaatac ctggtagaaa accagtttaa
     4201 aattgtagat gaaagcctag ccagagaagg ggaaagattt tatgaaataa ttctggctaa
     4261 aaaaggacca tcaaccatag aaaaagatat ttattatgaa cttggcccaa aattaataga
     4321 aaaaaaccat ccccttttaa ggacctacat tgaaaaaaag attttccaac taaacaatat
     4381 aaaaaacagc ctaaaaaagg ccaaggatgc tgaggcctta gaaaagaaaa gagatattat
     4441 agaaaaaata aaagcttata gggaggtgct agaaacaatt gaaagctaaa aggattattc
     4501 aagcaatgga taaatgggtt ccaagtaaac ttattgattc ctgggacaat acaggctttc
     4561 aaataggaga tgaagaaagg gaagttaaaa aagttttagt tgctcttgat ttagacaggc
     4621 ctaccttaaa gaaggctatt gaggagaact atcagatggt aataacccat catcctttaa
     4681 tctttaagcc tcttaatagg ataaactcta aagattacac aggaagatta atcatggaca
     4741 ttattaaaaa tgatatagta gtatataatg cccattcaaa tttggatatg gccatagatg
     4801 gggtaaatga tgaactagcc aatatattca acttaaaaga tagtaaaatt ttaagaaaag
     4861 tttatcatga tgaattggtg aaactggcag tttatacacc taaagactat gcagaccaaa
     4921 taagatttgc cctaggcaat agtggagctg gccatattgg caattatagc cattgtacct
     4981 ttaactcaga tgggataggt acctttcttc ctacagcaga tgcaaaaccc tatataggca
     5041 aggtaggtac cttggaaaag gtggaagagg taaaggttga aagtatttta aaaagggagg
     5101 acttatccca tatattggct gaagtaaaaa aggcccatcc ttatgaagaa gttgcctatg
     5161 atgtttatcc tctattaaat gaagggacag cctatggata tggtagggta ggatatattg
     5221 aagagactga ggcagaagag tttatatatc aggtgaaaga taaacttaaa gtatcagacc
     5281 taactgcata tgggaatata gatgggactg taagtaaagt agctttatgt ggaggtagtg
     5341 gtagtaactt catatacgat gcccacaagg ctaaggccga tatttatatt acaggggatc
     5401 tgaaatatca tgatgcccaa ttggcccagg aattaggaat gattttgtta gatgcaggac
     5461 atttccatac agaaaagatt attctgccgg ttataaaaaa atacctgttg gagaaattag
     5521 gggaggaact gagcattaag gttcttatgg agtcaagctt acctgaaaaa acctattaaa
     5581 ggctgggggt ctaattatga aaagtttaga ttttctttgg gcattagaag gtcacaccaa
     5641 tagtttggat gagttgaaaa aaagttatga tctattactt gaaaccat
//
LOCUS       DATGSI010000310         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_494305, whole genome shotgun sequence.
ACCESSION   DATGSI010000310 DATGSI010000000
VERSION     DATGSI010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494305"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VK031_04835"
     CDS             <1..1197
                     /locus_tag="VK031_04835"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLS53276.1"
                     /translation="EIIETFADYTSILTGVDFGTVTVVIDKNNIEITTHRIEEEYIDG
                     RKPSKVHFTNDLEGDLSRRDFTINAMAFNGEFGLIDPFKGQKDLKDKLIKTVGNPRER
                     FEEDHLRILRAIRFATQLNFTIEENTYKACKDMGKLLRSISVERIRDELFKILLSEKP
                     SYGIKLMKDLEILEVILPELLPTIGFDQRNPHHSKDVFYHTLCVVDNTPTILSVRLAA
                     LFHDLGKPHTLSIDEEGVGHFYGHEKVSEDIARKRLNGLNCSNQLIKRVTSLVREHMT
                     HHANYKDKGLKRLIRRVGKDNIFNLIDLQKADKMCSNPQADITLLNNREKQIKRILDS
                     KEPYEKKQLAIDGSDILKLGYKEGKIIGEILDYLMEKVLEEPKLNNKDDLLRLIMMEF
                     ESDEEF"
     gene            1184..1354
                     /locus_tag="VK031_04840"
     CDS             1184..1354
                     /locus_tag="VK031_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53277.1"
                     /translation="MRNFRFILLGLPLLSEKNSPLIMLTVLGTMFNSIFMMIMLRRIE
                     IKYKKDQDESKF"
     gene            1476..>1615
                     /locus_tag="VK031_04845"
     CDS             1476..>1615
                     /locus_tag="VK031_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HLS53278.1"
                     /translation="MEKLFGTDGIRGIANKDLTPELAFKVGRAGGYILSKGNKGKILV
                     GK"
BASE COUNT          641 a          198 c          333 g          443 t
ORIGIN      
        1 gagattattg aaacttttgc tgattataca agcatattaa caggagtaga ctttggtact
       61 gtaacagtgg ttattgataa gaataatata gaaataacta cacatagaat agaagaagaa
      121 tatatagacg gtagaaaacc ttcaaaagtc cattttacca atgatctaga gggggattta
      181 agtcgaaggg actttaccat aaatgccatg gcctttaatg gggaatttgg tttaatagat
      241 cccttcaaag gtcagaaaga cttaaaagac aaattaataa aaactgttgg caatcctaga
      301 gaaagatttg aagaagacca tttgagaatt ttacgggcca taagatttgc aacccagcta
      361 aatttcacca tagaagaaaa tacctataag gcctgtaagg atatgggaaa gcttttaaga
      421 agtattagtg tagaaagaat cagagatgaa ctatttaaaa tactattatc agaaaaaccc
      481 tcctatggaa taaaattaat gaaggattta gagattttgg aagtaatatt accagaatta
      541 ttaccgacca taggatttga tcaaagaaat cctcatcaca gtaaagatgt gttttatcat
      601 accttatgtg tagtagacaa tactccaacc atattaagtg tgaggctggc agccctgttt
      661 catgacctag gaaagcctca tacattaagc atagatgaag agggagtagg acatttctat
      721 gggcacgaaa aggttagcga ggacatagct agaaaaagac taaatggact gaattgttcc
      781 aatcaattga ttaaaagggt tactagtttg gttagggagc atatgaccca tcatgccaat
      841 tacaaggata aggggctcaa aagattaatc agaagagtgg gaaaggataa tatatttaat
      901 ctaatagact tgcaaaaggc agataaaatg tgctcaaacc cacaggcaga tattacactc
      961 ttaaataata gggaaaagca aattaaaaga atattagatt caaaggaacc ctatgagaaa
     1021 aaacaacttg ctattgatgg cagtgatata ttaaaattag gttacaaaga aggaaaaata
     1081 ataggagaaa ttttagacta tttgatggag aaggtattgg aagagcctaa gttaaataat
     1141 aaggatgact tattaaggct tattatgatg gagtttgagt cagatgagga attttagatt
     1201 tattcttttg ggattaccac ttttatcaga aaaaaactca ccactaataa tgttgactgt
     1261 gttaggaacc atgtttaatt ctatatttat gatgattatg ttaagaagga ttgaaattaa
     1321 atataaaaaa gatcaggatg aatccaagtt ttaatgctaa atcatcggcc aagcaaaatc
     1381 aatataagta tccaaaaggt aaaagtaggg gcttatataa acaaatttta gaatatggat
     1441 acttgattaa ttgagaaatt agagggggaa aacaaatgga aaagttattt ggtacagatg
     1501 gaatacgagg aatagcaaat aaagatttaa caccagagct ggcctttaag gttggacgag
     1561 caggtggtta catattatct aagggaaata agggaaaaat actggtaggt aagga
//
LOCUS       DATGSI010000311         1040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_67876, whole genome shotgun sequence.
ACCESSION   DATGSI010000311 DATGSI010000000
VERSION     DATGSI010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67876"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VK031_04850"
     CDS             <1..1010
                     /locus_tag="VK031_04850"
                     /inference="COORDINATES: protein motif:HMM:NF033529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HLS53279.1"
                     /translation="EEAIDSPYRKSGQKVSDSLELTGQTVMNSIRELGPVENNTVPIK
                     EKNKEVKLLFIEADEDHVSLQNGKNIEPKLVYVHEGRKKVGKNRYKLINKRVFSGVYK
                     SSEDLWLEVADYIDEAYDLDKIEKIYLSGDGASWIKTGVEWIKSSIYVLDRFHLSKYV
                     KRATAHLPHITSSLWNYINRLEKDNVKELFKIILEETESETKKESVRDCRRYILNNWE
                     GIKPQYNDDYIGCSAEGHVSHILSDRLSSRPLGWCVEGVDQMSRLRAFKSNGGNVYTL
                     FIQRRKQELKEERILKLEKRSIKKKIILTSANEVMGNIPLLQDGRNSGMRTILKSISG
                     A"
BASE COUNT          446 a          121 c          220 g          253 t
ORIGIN      
        1 tagaagaagc aatagactca ccctatagaa aaagtggaca gaaagttagt gacagcctag
       61 agttaacagg acaaacagta atgaattcaa ttagagaatt aggtccagta gaaaataata
      121 cggtaccaat aaaggaaaag aacaaagaag taaaactact tttcattgaa gcagatgaag
      181 atcatgtatc ccttcaaaat ggtaaaaata tagaacctaa acttgtatat gtgcatgaag
      241 gtagaaagaa ggtaggaaaa aacagatata aattaataaa taaaagggtg tttagtggag
      301 tatataaatc aagtgaagac ctatggttag aagtagcaga ctatatagat gaggcctatg
      361 atttagacaa aatagaaaaa atatatctat caggagatgg agcaagctgg ataaagacag
      421 gggtagaatg gataaaaagc tcaatatatg tactagacag atttcatcta tctaagtatg
      481 tcaaaagggc tacagcacat ttaccacata taactagctc attatggaat tatattaata
      541 ggttagaaaa agacaatgta aaagaactat ttaaaattat actagaggaa acggaatcag
      601 aaactaaaaa ggagtctgta agagactgta ggagatatat tttaaacaac tgggaaggta
      661 taaaaccaca atataatgat gactatatag gctgtagtgc tgagggacat gtaagtcaca
      721 tattatcaga tagattaagc tcaagacctc taggctggtg tgtagaaggc gtggaccaga
      781 tgtcaagact tagggcgttt aaatctaatg ggggaaatgt atacactcta tttatccaga
      841 gaaggaaaca agaattaaag gaagaaagaa ttttaaagtt agagaaaaga agtattaaga
      901 agaagataat attaacatca gccaatgagg taatgggaaa catacctcta ctacaagatg
      961 gaagaaatag tggtatgagg actatactta aatcaatcag tggtgcttag attaagaaga
     1021 atgcaactaa aattatattg
//
LOCUS       DATGSI010000312         5545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_253317, whole genome shotgun sequence.
ACCESSION   DATGSI010000312 DATGSI010000000
VERSION     DATGSI010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253317"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VK031_04855"
     CDS             <1..432
                     /locus_tag="VK031_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HLS53280.1"
                     /translation="PITIPGAKLGLSNIVVLVTLVIFGFKEGIVVSILKSSVLMLVTG
                     SVSSFIYSISGAIFSCIIMFIAYRFLSKIFSLIGVSILGALSHNTAQITVASLMMNNL
                     RIFTYLPFLMLTSLFTGYFVGLASRYITRNLKKNINILLEK"
     gene            444..1019
                     /locus_tag="VK031_04860"
     CDS             444..1019
                     /locus_tag="VK031_04860"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HLS53281.1"
                     /translation="MDIILASASPRRREILEKYNLKHRVKKSDIIEHIRPDESPEQIA
                     MSLAFQKAYNIALENKKSIVIGADTIVVYDNRILGKPTCKKEAKEILNLLNGQKHQVI
                     TAIALVLLDKNIKIVDYEETLVKFRDLDEDIIDRYVKTREPFDKAGAYGIQDYGALLV
                     EEIRGCYLNVVGLPLSKLNNLLNIYFNISLL"
     gene            1045..1743
                     /gene="radC"
                     /locus_tag="VK031_04865"
     CDS             1045..1743
                     /gene="radC"
                     /locus_tag="VK031_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HLS53282.1"
                     /translation="MDNFERNYTIKDLPVSERPREKLYSYGPESLSNAELLAIIIRTG
                     HRQDTALDVAQRILSMDNRGLTYLSEVSIEQLMEVKGIGKCKAAQILAAIEIGKRLSR
                     WGAHEKTRVNSPVVLANLVMDEMRYLNKEHFRIAILDTKNQILSIENISIGTLNASIV
                     HPRDVFYAAIRRNANSIILIHNHPSGDPEPSKEDISITNRLIQVGDLLGIKVLDHIII
                     GDNRYISFKEKNLI"
     gene            1765..2796
                     /locus_tag="VK031_04870"
     CDS             1765..2796
                     /locus_tag="VK031_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HLS53283.1"
                     /translation="MGFFSFFNKDMGIDLGTANTLVYVKGKDIVTREPSVVAIQTSTN
                     QVLAVGEEAKKMIGRTPGNIVAIRPLKDGVIADFDVTQSMLKHFIRKAYKRRSLFQPR
                     VVVCVPSGVTEVEKRAVEEAAIHAGARDAYLIEEPMAAAIGAGLPVHEPTGSLIVDIG
                     GGTTEVAVISLGGIVTSKSIRVGGDEFDESIVNYIKKQYNLMIGERTAEEIKITIGSA
                     DVESEVKTMDVRGRDLVSGLPKTIEVTSSEIYEAMEEPIYNIIEAIKSTLEKTPPELA
                     ADIMEQGIMLTGGGSLLEGIDRIIIKETGMPVNIAENPLDCVANGTGMALDSIEVLKK
                     SVYTSKRIG"
     gene            2840..3643
                     /gene="mreC"
                     /locus_tag="VK031_04875"
     CDS             2840..3643
                     /gene="mreC"
                     /locus_tag="VK031_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HLS53284.1"
                     /translation="MVTAVAIILIVIIGLTSKERMSLTKFENFFGNALRPIKKVGYSV
                     GNRINTSLESVLNVTRLSKENKELKIEVARLREENANLENLIGQSDILINEAQLIEET
                     KYKLLPAQVTGKQPGNWYDVFTIDKGLKDGLSKGDTIIQAIELDKDTIYEGLVGRVVD
                     VGNNWAKVVSIIDELNKMGFKIIRTQDGGILSGSVDNVLSGYLYDNEADVIVGDKLYT
                     SGIGTVFEKDIYIGEVEEVIEVEEELTKKIIVKPAVDFKKLYRVFAIIE"
     gene            3658..4158
                     /gene="mreD"
                     /locus_tag="VK031_04880"
     CDS             3658..4158
                     /gene="mreD"
                     /locus_tag="VK031_04880"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HLS53285.1"
                     /translation="MAIVTMIIIILVNLILQSTLLPFLNFLKYLPNLGLVSVVAIGLI
                     RGRYYGGFFGLALGLAQDILFGRVIGVNAFIYFMLGYLVGYVQASLNQENLAIPLTFS
                     ALGTIFYNFLYFLFMYFLSIDIEFSTALTFIFSIEILYNGLISIFVYKLFTKIIKVPS
                     LRFGRR"
     gene            4170..>5545
                     /locus_tag="VK031_04885"
     CDS             4170..>5545
                     /locus_tag="VK031_04885"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53286.1"
                     /translation="MNFLEKLKDRYTVITIVLVILISTLSFRLATLTIAQGDYYRNLS
                     DNRRVKEVYTTAPRGEIRDRYGRLLAGNKPSFTVQLFKDEINLYSREEKNEAFLTLVR
                     LLEEDGVNYIDDFPIDLNTIKYKSEKDYQEESLSPMDKVIELVLENNLLGEVLNSYYL
                     YEGYDEHFKFMTVNKAVNALKRKGMDVPIDGSFENGKPQIKFKENKNIKQWKESEGLA
                     ADDSPIKALIKLIDKDKAIIRQTIDNPIARKLTYEILKSKNLADNLILEEFSITHMEQ
                     YLDQKRSLNKLYPEVTLETSAEEDFVNIFTRSSLKNFLQVSFTIEEEGKEKVIIPAQI
                     LIDMLKEKKVEVPLKVEVAEGGNVVFYYTDAKAREEEDLVELLIEYGKENSILADFIT
                     SETIRSLAQEQLLTDGINPRISIAQDIEYVAINNLNKFYTENKLDHDLTVEEIFNKLI
                     KNYEVDEN"
BASE COUNT         2044 a          878 c         1140 g         1483 t
ORIGIN      
        1 cctataacaa taccaggggc aaagcttggt ttatccaata tagttgtgct ggtgaccttg
       61 gtaatttttg ggtttaagga aggcatagta gtatcaatac ttaaaagttc agtccttatg
      121 ctggttacag gaagtgtatc aagctttatt tatagtattt caggagccat attttcctgt
      181 ataataatgt ttatagccta taggttttta tcaaaaatat ttagcctaat aggtgtaagc
      241 atccttgggg ccctatcaca taacacggcc caaattacag tggccagcct aatgatgaat
      301 aatttaagaa tatttaccta tttgcctttt ttaatgttga ctagcctgtt tacaggctac
      361 tttgtaggcc tggcatctag atacataact agaaacctaa agaaaaatat aaatatatta
      421 ctggaaaaat aaggatgaag attatggata taatactggc atcagcttct ccaagaagaa
      481 gagaaatttt ggaaaagtat aatcttaaac atagggtcaa aaaaagtgat ataattgagc
      541 atataaggcc tgatgaaagc ccagaacaaa tagccatgag tctggccttt caaaaagcct
      601 ataatatagc tttagaaaat aaaaaaagta ttgtaatagg tgccgatact atagtagtat
      661 atgataatag gatattaggt aaaccaacct gtaaaaagga agccaaagag atcctaaatc
      721 tcttaaatgg ccaaaagcac caggttatta cagccatagc cttagtccta cttgataaaa
      781 atataaaaat agtagactat gaagaaactt tggtaaaatt tagagacctg gatgaagaca
      841 taatagatag atatgtaaaa accagagaac cctttgataa ggctggagcc tatgggatac
      901 aagactatgg agccctatta gttgaagaaa taagaggctg ctacttaaat gtggtaggct
      961 tacccctatc taaactgaat aacttactaa atatatattt taatatatct ttattataat
     1021 atataggagt aggtgaacct ttatatggat aattttgaaa gaaattatac tattaaagat
     1081 ttaccagtaa gtgaaagacc aagggaaaaa ctttacagct atggacctga atccttatcc
     1141 aatgctgaac ttttggcaat tattataaga actgggcaca ggcaggatac agccctagat
     1201 gtggcccaga gaatactaag tatggataat agaggactta catacttgtc tgaagtttcc
     1261 atagagcagt taatggaggt aaaaggaata gggaaatgta aggctgcgca aattttagca
     1321 gccatagaaa taggcaaaag gttaagcaga tggggagccc atgaaaagac tagggtaaat
     1381 tcacctgtgg tcttggcaaa tttggtcatg gatgagatga ggtatctaaa taaggagcac
     1441 tttagaatag ctatactaga tactaaaaac caaatcttat caatagaaaa tatatctatt
     1501 ggcaccctga atgcatccat agttcatcca agggatgtat tttatgcagc catcaggaga
     1561 aatgcaaatt caattatcct aatacacaat catccaagcg gtgaccctga accaagcaaa
     1621 gaagatataa gtattaccaa tagactgata caagttggag atttacttgg tattaaggta
     1681 ttggatcata taattattgg tgataataga tatataagct ttaaagagaa aaatttaatc
     1741 taaagattgg aaggagaaaa ttacatggga ttttttagct tttttaataa agatatggga
     1801 atagacttag gaacagcaaa taccttagta tatgtaaaag gaaaagatat agtaaccaga
     1861 gagccttctg tagtggccat ccaaaccagc accaaccaag tattggcagt tggagaagaa
     1921 gccaaaaaaa tgattggaag gactccagga aacattgtag ccatcaggcc cttgaaagat
     1981 ggagttatag cagactttga tgtgacccag agcatgctta aacactttat ccgcaaggca
     2041 tataaaagaa gatctctatt ccagcctagg gtagtagtat gtgtacccag tggagtaaca
     2101 gaagtagaaa aaagggctgt agaagaagca gccatccatg ctggagccag ggatgcctac
     2161 ctaatagaag aaccaatggc agcagccata ggggcaggtc ttcctgttca tgaaccaaca
     2221 ggtagcctga tagtagacat aggtggagga actacagaag tagcagtaat ctccctagga
     2281 ggcatagtaa ccagcaagtc catccgagta ggtggagatg aatttgacga atctatagtt
     2341 aactatataa agaaacaata caacctaatg attggtgaaa ggactgcaga ggaaattaaa
     2401 atcaccatag gttctgcaga tgtagaaagc gaagtaaaaa ccatggatgt aagaggtagg
     2461 gacctggtaa gtggcctacc aaaaactatt gaggttacat ccagtgaaat atatgaagcc
     2521 atggaagagc ctatctataa tataatagag gctattaaat caaccttgga aaaaacacca
     2581 ccagagttgg cagcagacat tatggaacaa gggattatgt taacaggtgg cggatcctta
     2641 ttggagggaa ttgataggat aatcattaaa gaaacaggca tgccagtaaa catagcagaa
     2701 aatcccttag actgtgtagc caatggaaca ggtatggcct tagatagtat agaggtattg
     2761 aaaaagagcg tatatactag taaaagaata ggttagtggt gataatatgt atttcttccg
     2821 caaatacaga gatagaatga tggtgacagc tgttgctatc attctaattg ttataatcgg
     2881 ccttactagt aaagaaagaa tgtctcttac aaagtttgaa aacttctttg gcaatgccct
     2941 aaggcctata aaaaaggttg gctatagtgt aggaaatagg ataaatactt ccctggaatc
     3001 tgttttaaat gttaccaggc tttcaaagga aaataaagag ttgaaaatag aagtggccag
     3061 actcagggaa gaaaatgcaa acttggaaaa tcttatagga caatcagata tccttataaa
     3121 cgaggcccaa ttgatagaag aaacaaaata caagctttta cctgcccagg ttacaggcaa
     3181 acaacctggt aattggtatg atgtttttac catagacaag ggcctaaagg atggcctatc
     3241 taagggagac acaattatcc aggccataga gctggataag gataccattt atgaaggctt
     3301 ggtaggccgg gtagtagatg taggcaataa ctgggccaag gtagtatcca taatagatga
     3361 gcttaataaa atgggcttta aaatcataag aacacaagac ggaggtatcc tatctggtag
     3421 cgtagacaat gtattaagtg gatacttgta cgataatgag gctgacgtaa tcgtaggcga
     3481 caagctttat acctcaggca ttggcactgt ctttgaaaaa gatatttata taggagaggt
     3541 agaagaagta atagaagtag aggaagagct aaccaaaaag attattgtta agccagctgt
     3601 agactttaaa aagttataca gagtatttgc cataatagaa tgatttgagg gataagattg
     3661 gcaattgtaa ccatgattat tataatttta gtaaatttaa tactgcaaag caccctatta
     3721 ccctttctta acttcctaaa gtatttacca aatctaggtc tggtttcagt agtggccata
     3781 ggcctgatta gaggtagata ctatggaggc ttctttggat tggcccttgg actagcccag
     3841 gacatcctct ttggtcgggt aataggcgta aatgctttta tctattttat gctaggatat
     3901 ttagtaggtt atgtgcaggc ttcattaaac caagaaaacc tagctattcc ccttactttt
     3961 tcagcactag gtacaatatt ctataatttc ctatattttt tattcatgta ctttctttcc
     4021 atagatatag aattttccac agcactaact ttcatatttt ccatagaaat tctatacaac
     4081 ggcttaatat caatatttgt ctataaacta tttacaaaaa taattaaggt accatcctta
     4141 cgttttggaa ggaggtagag gtgatttaaa tgaatttctt agaaaaactt aaagatagat
     4201 atacagtcat aaccatagta ttagtaatcc ttatatccac cctgtccttt aggctggcta
     4261 ccttaaccat agcccagggt gattattata ggaacctatc agacaacaga agggtaaagg
     4321 aggtctatac tacagcccct agaggggaga ttagagatag atacggtagg cttttggcag
     4381 gcaataagcc tagctttact gtccagcttt ttaaggatga aataaaccta tatagcaggg
     4441 aggaaaaaaa tgaagccttc ctgaccttgg taagactttt ggaagaagat ggggttaact
     4501 atatagatga ctttcccatt gatttaaata caatcaaata caaaagtgaa aaagactacc
     4561 aggaagaaag cctaagccct atggataagg taatagaatt agtcctagaa aacaacctgc
     4621 taggagaggt tttaaattcc tactaccttt acgaaggtta tgatgaacac tttaaattta
     4681 tgacagtaaa caaggcagtc aatgccctaa aaagaaaggg tatggatgtg cccatagacg
     4741 gtagctttga aaatggcaag ccacagatta aatttaaaga aaacaaaaac ataaaacaat
     4801 ggaaggaatc tgaaggcctg gctgccgatg attctccaat aaaagcttta atcaaactaa
     4861 tagacaagga caaggccata atcaggcaaa ccatagataa cccaatagcc aggaaattga
     4921 cctatgaaat attaaaatct aaaaacttag cagacaatct aatcctggag gaattttcca
     4981 taacccatat ggagcagtac ctggatcaga aaagaagctt aaataaacta tatccagagg
     5041 taacacttga aaccagcgca gaagaagact ttgtaaatat atttactaga agctccttaa
     5101 aaaacttcct acaggtaagt ttcaccatag aagaggaagg taaggaaaaa gtcataattc
     5161 cagcccaaat ccttatagat atgctaaagg aaaaaaaggt ggaagtaccc ttaaaggtgg
     5221 aagtggccga aggaggcaat gtggtctttt actacacaga tgccaaggcc agggaagagg
     5281 aggatctagt agaactacta atagagtatg gcaaagagaa ttctatttta gccgatttta
     5341 taactagtga aaccatcagg tccttggccc aggagcaatt actgacagat ggcattaatc
     5401 ctagaatatc catagcccag gatatagaat atgtggcaat caataatcta aacaaatttt
     5461 acacagaaaa caagctggat catgatttaa ctgtagaaga aatctttaat aaattaataa
     5521 aaaattatga agtagacgaa aactt
//
LOCUS       DATGSI010000313         1478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_637075, whole genome shotgun sequence.
ACCESSION   DATGSI010000313 DATGSI010000000
VERSION     DATGSI010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637075"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VK031_04890"
     CDS             <1..485
                     /locus_tag="VK031_04890"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HLS53287.1"
                     /translation="KLNDIGIYTIEDLLDLSEDFLIELFGKHGSEIYDRIRGIDKRKV
                     DIDRERKSLGTETTFSQATRDKGRLCTYLKSFSKEVSISLIEKELQGRTITVKVKYDD
                     FSIRTKSRTLFHNTNDEEEIFELACSLLEEIKLDKKVRLIGLTVSNLSSLDFKQLSFI
                     "
     gene            608..>1478
                     /locus_tag="VK031_04895"
     CDS             608..>1478
                     /locus_tag="VK031_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLS53288.1"
                     /translation="MERREFKAESKRLLDLMINSIYTHKEIFLRELISNSSDALDKLY
                     YKSLVDKSINLNKEDFYIKVSLDKANRSLRVYDTGIGMTKEELEENLGVIAKSGSFAF
                     KNENKDLDEVDIIGQFGVGFYSAFMVADTVTVISRAYNSPKAYKWQSSGADGYTIEEA
                     ERSNHGTEIILKLKESTDEEDYDEFLNETRIEGIIKKYSDFIRYPIKMKVTKSRLKEG
                     SKDEYESYQKEEIINSMVPIWRKNKNELTEEDYINFYQEKHFGFDEPIKYIHLSMDGL
                     ISYNSILYIPSRVP"
BASE COUNT          562 a          191 c          308 g          417 t
ORIGIN      
        1 gtaaattaaa tgatatagga atatatacca tagaggactt attagatctt tcagaagatt
       61 ttctaattga gctctttggt aaacatggta gtgaaatata tgatagaatt cgaggcatag
      121 ataagagaaa ggtagatata gatagggaga gaaagtcctt gggaacagag accaccttta
      181 gccaggcaac cagggataag ggaaggctgt gtacctactt aaaaagcttt tcaaaagagg
      241 tctcaatttc cctaatcgag aaagagttac aaggtagaac tattacagtt aaggtaaagt
      301 atgatgattt tagcataagg acaaaaagca gaaccttgtt tcacaatacc aacgatgagg
      361 aggagatatt tgaactagcc tgtagccttt tagaagagat caagctggat aaaaaggtta
      421 gattaatcgg acttactgta tccaacttat catctttgga cttcaaacaa ttatcattta
      481 tctgatattt ataagatatg gaccatttga cactaaagga aaaatatgtt atatatttca
      541 tataattagc actcattaat taagagtgct aattatattg gaaattttta caaggaaggg
      601 gaaaagtatg gagagaagag aatttaaggc agaatcaaaa agattgttag atttaatgat
      661 taactctatc tacacacata aggaaatctt tttaagagag cttatttcaa attccagtga
      721 tgcccttgat aaactttatt acaaatcgtt agttgataaa agtattaatc ttaataagga
      781 agatttctat attaaggtaa gccttgataa ggccaataga agtctaaggg tttatgacac
      841 agggattggg atgacaaaag aagagttgga agaaaatcta ggggtaattg ccaaaagtgg
      901 ctcctttgcc tttaagaatg aaaataagga cttagatgaa gtagatataa ttggacaatt
      961 tggggtaggc ttttattcag cctttatggt ggctgatact gttactgtaa taagtagggc
     1021 ctataacagc cctaaggctt ataaatggca gtccagtgga gccgatggct atactataga
     1081 agaggctgag aggagcaatc atggaaccga gattatacta aagttaaagg aaagcacaga
     1141 tgaagaagat tatgatgaat tcttaaatga aactaggata gaaggcataa ttaaaaagta
     1201 ttctgacttt atcaggtatc ctataaaaat gaaggtgacc aagtcaagat taaaggaagg
     1261 ttccaaagat gaatatgaaa gttatcagaa agaagagatc ataaatagta tggtccccat
     1321 ttggagaaag aataaaaacg agcttacaga agaggattat ataaatttct accaggaaaa
     1381 gcacttcggg tttgatgaac ctataaaata tatacattta agcatggatg gtttaatcag
     1441 ctacaattcc atcctttata taccaagcag agttccct
//
LOCUS       DATGSI010000314         1383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_68257, whole genome shotgun sequence.
ACCESSION   DATGSI010000314 DATGSI010000000
VERSION     DATGSI010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68257"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VK031_04900"
     CDS             <1..231
                     /locus_tag="VK031_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLS53289.1"
                     /translation="NQVDLRLETKKLYRCIDTVLKDRERVIVQLRYGLIDGEYRTQRE
                     IANILGISRSYVSRIESRAIKKLSKAMEKSKI"
     gene            complement(267..944)
                     /locus_tag="VK031_04905"
     CDS             complement(267..944)
                     /locus_tag="VK031_04905"
                     /inference="COORDINATES: protein motif:HMM:NF021120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhcN/YlaJ family sporulation lipoprotein"
                     /protein_id="HLS53290.1"
                     /translation="MKINKFRFIALGLVLLLGLVACAPADTGLDNRQRRLTTQTRIGD
                     GARRDSGTNTQENDYPNVGQDNMIDRDGLLGQDNNLMDNSMGLGNRRDNLNTDLNDGM
                     TRPNLDTNLTGDRGRGSEEIAKKISDLPEIDKASVVINNDTALVGCKLRGDTQGTMTT
                     TLRDKIERIVRDTDSNIKNVSITTEPDLYKRIKTMATDLDRGNPVEDFADEIRDLIRR
                     ITPNTNR"
BASE COUNT          479 a          215 c          197 g          492 t
ORIGIN      
        1 aatcaagttg acttaaggtt agagactaaa aagttatata gatgtattga tacagtttta
       61 aaagataggg aaagggtaat agtccaatta cggtatggct taatagatgg ggaatatagg
      121 actcaaagag aaattgccaa tatcttagga atttccagat cttatgtttc tagaatcgaa
      181 tcaagagcta taaaaaaatt atctaaggcc atggaaaaaa gcaagatata aaggctcgat
      241 ttaactagta aatcgagcct ttgtgtttat ctgttagtat ttggagtaat tcttcttatt
      301 aaatctctaa tttcatctgc aaaatcttcc acaggatttc ctctatctaa atctgtagcc
      361 atagttttta ttcttttata taagtcaggt tctgtagtta tggatacatt tttaatgttt
      421 gaatctgtat ccctaactat cctttcaatt ttatctctta gagtagtggt catagtacct
      481 tgtgtatctc ctcttaattt acatcctact aaagcagtat cattgttgat cactactgaa
      541 gccttatcta tttcaggtag atctgatatc tttttagcta tttcttctga acctctacct
      601 ctatcaccag ttaagttagt atctaggtta ggtctagtca ttccatcatt taaatctgta
      661 tttaagttat ctcttctatt acctaatccc atactattat ccataagatt attgtcctga
      721 cctagtaagc catccctatc tatcatattg tcttgaccta catttggata atcattttcc
      781 tgggtattag tcccactatc tcttctagca ccatctccta ttctagtttg tgtggtcagc
      841 cttctttgtc tattatctag gccagtatca gcaggagcac aagcaaccag ccctaaaaga
      901 agtactaatc ctaaagcaat aaatctaaat ttatttattt tcaaatgttc cacctcctat
      961 caatagtctg gcacagaaac ttaaatttat ttaatctaat ttttaagtta attgtgtaat
     1021 ctatccaagg attaacataa gaaatcttat cataattgat aaaaatataa attaagaaga
     1081 aactttaaaa ctttatttgg ccttataact acctttgcgg ttgaatttat gatatattgt
     1141 acttagtaca aattgtttaa caaaagaaag ttttaaaaat aattgaaaat aaagataaat
     1201 ttcaaaagag gattaataat gaaaaataaa agacacaatt taagattagg tgagttatta
     1261 ttatatacaa ataaaataaa ttataatcag ttagaaattg ccattcaaga acaaaaaacc
     1321 agtactaaaa aaattggtca aattttaatg gacaaggggt ggattactga agaggatatt
     1381 atg
//
LOCUS       DATGSI010000315         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_637383, whole genome shotgun sequence.
ACCESSION   DATGSI010000315 DATGSI010000000
VERSION     DATGSI010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637383"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VK031_04910"
     CDS             <1..225
                     /locus_tag="VK031_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HLS53291.1"
                     /translation="VNVFAKAEASPDGLVRDRRTTMLTDSDINHTRHARAVVNAVIAS
                     LVGDPMVYVSGGAEHQGPAGGGPVAVIIEK"
     gene            242..1438
                     /locus_tag="VK031_04915"
     CDS             242..1438
                     /locus_tag="VK031_04915"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLS53292.1"
                     /translation="MKYILKNAYFPFDNQIKDIYVEAGIVKDIGHGLNVESATVIDLE
                     KRMVIDGFVDGHMHLDKALIADKISNKSGTLEEAISIMSNYKPEMSKEDIENRARKVL
                     DMALAQGTRYARTHVDVDESAGLKSMEVLLKLREEYKEKINIQIVAFPQEGIVESKKS
                     YAYLDEALKLGADVVGGIPAIEKDPLKHMEMIFNLALKYDVDIDMHIDETDDPKSLTL
                     RDLAKMTIDNDYEGRVTAGHCCSLAANPKDKILPIIELVKEARIGIISLPSTNLYLQG
                     RGDSYNVRRGITPVKFLVEEGVNVLIGSDNIRDPFNPFGNSNLLEELLIAAHGVHMGG
                     EKDLNKLFDMISIVPGKVLGFDYGIREGKRANFVVVNASNKASAIIGQVGMFGYFEEG
                     KFKTNE"
     gene            1456..>2015
                     /locus_tag="VK031_04920"
     CDS             1456..>2015
                     /locus_tag="VK031_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HLS53293.1"
                     /translation="MSLFINIIPLAVILLLLFRKQHMLFAGLVGGVIAIIIGKMALPQ
                     ASGMFVDGINNMLSITIPIIYAASAAMVSEAGSIEALVELSRRGLGGRVSILAGIIVL
                     IQGFATYMAGMGAGNSMVTAPLAATAVGAVPEVIAAMAIASAVGFTTSPAPTETVLAA
                     ESAGRDVVSHATAMRPYTVLFYLLAAG"
BASE COUNT          646 a          321 c          504 g          544 t
ORIGIN      
        1 gtaaatgtct ttgccaaggc cgaggcctcg ccagatggat tggtaagaga tagaagaacc
       61 accatgttaa cagattcaga tattaaccat acccgtcatg ctagggcagt agtaaatgct
      121 gtaattgctt ccttggttgg agatccaatg gtttatgtat caggtggagc agaacaccaa
      181 ggtcctgctg gaggaggtcc tgtggcagtt attatagaaa aataagaaat ggagtgagaa
      241 gatgaaatac attttgaaaa atgcttattt tccttttgat aaccaaataa aagatattta
      301 tgtagaggca ggaatcgtta aagatatagg acacggcctt aatgtagaaa gtgcaacggt
      361 catagacttg gaaaagagaa tggtaataga cggttttgta gatggccata tgcacttaga
      421 caaggccctg attgcagata aaatttccaa caaaagtgga accttagagg aagcaatcag
      481 cattatgagc aattataagc cggaaatgtc caaggaagat atagaaaaca gggctagaaa
      541 ggtcctagat atggccttgg cccagggtac tagatatgcc aggacccatg ttgatgtaga
      601 tgagtctgca ggtcttaaat ctatggaagt tcttttaaaa cttagggaag agtataagga
      661 aaaaataaat atccaaattg ttgcctttcc ccaagaaggt atagttgaaa gtaaaaaatc
      721 ttatgcttac ctggatgagg ccttaaaatt aggggcagat gtggtaggtg ggataccagc
      781 catagaaaag gatcctctta aacatatgga aatgatcttt aacctggccc taaaatacga
      841 tgtggacata gatatgcata ttgacgagac tgatgatcct aaatccctaa ccttaaggga
      901 cttggccaag atgaccattg acaatgatta tgaaggtagg gtgactgctg gccattgctg
      961 tagtttagca gccaatccca aggataagat tttaccgatt attgagctgg taaaagaagc
     1021 aaggataggt ataatctcac tgccatcaac caacctatat ctacagggta gaggtgatag
     1081 ctacaatgtg aggcgaggta ttacgccagt taaattcctg gttgaagaag gagtaaatgt
     1141 gctgataggt tccgacaata taagggatcc tttcaatccc tttggaaata gtaatctatt
     1201 ggaggagtta ttaatagcag cccatggagt tcacatgggt ggagaaaagg atttaaataa
     1261 attatttgac atgataagca ttgtgccagg aaaagttcta ggatttgatt atggaattag
     1321 ggaaggaaaa agggcaaatt ttgtagttgt aaatgcatca aataaggcca gtgccattat
     1381 aggccaggtg ggaatgtttg gctactttga agaaggaaaa tttaaaacaa atgaataaat
     1441 gaaggaggca gtagcatgag cttatttata aacataatac cattggcggt aatattgcta
     1501 cttttattta ggaaacaaca tatgttattt gcaggactgg taggtggagt tatagcaata
     1561 attatcggga agatggcttt accacaggca tctggcatgt ttgtggatgg aataaacaat
     1621 atgttaagta taactatccc tattatttat gctgccagtg cagccatggt ttctgaggct
     1681 ggaagcattg aggccctagt agagttatcc aggagggggc taggtggaag ggttagtatt
     1741 ttagctggga ttatagtatt gatccaggga tttgctacct atatggcagg tatgggggca
     1801 ggtaattcca tggttacggc ccccttggca gctacagctg taggtgcagt tcctgaagta
     1861 attgcagcta tggccattgc atcagctgtt ggctttacca catcaccagc tccaacagag
     1921 actgtactgg ctgcagaaag tgcaggcagg gacgtggtgt ctcacgcaac agccatgaga
     1981 ccttatacag tactttttta tctattggcg gctgg
//
LOCUS       DATGSI010000316         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_590808, whole genome shotgun sequence.
ACCESSION   DATGSI010000316 DATGSI010000000
VERSION     DATGSI010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590808"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VK031_04925"
     CDS             complement(<1..361)
                     /locus_tag="VK031_04925"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HLS53294.1"
                     /translation="MDYIGITMGDPAGIGCEITLKALKNEDFREKSIIFGSRDLVEHF
                     LELLDMDEKINLISDIDEFDPASINLVNVLDIRLDDFEFGKVSALCGDAAYRYVEKAI
                     EWALRGKIKGVVTAPLNK"
     gene            complement(454..1308)
                     /locus_tag="VK031_04930"
     CDS             complement(454..1308)
                     /locus_tag="VK031_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLS53295.1"
                     /translation="MGIVLILVSSFFFTLSSMFGKIVTSTTNMSGILSAFFRFSIGAI
                     LMFAYIMFKGKSFKSDNMKPMILRGIFNSLAMITFSLGFQYTTITNTNMLHMSYPVFV
                     VLFAPYFTKEKIKKSSYLYLTIIMLGSYIVANPSFGYINIGDFFAFLSAVVGAFSILY
                     LTIASRDNESYLIVFYVMLVGTLVNLPLVLRDFATFEMAGLLPALLSGICGVLGQIFI
                     TLGYRYVDSATGSMVANSRVVMGAILGYIFLDETIDLRIIIGMVLIIGALVGLSGYFS
                     KDRKEAVE"
     gene            complement(1392..1553)
                     /locus_tag="VK031_04935"
     CDS             complement(1392..1553)
                     /locus_tag="VK031_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLS53296.1"
                     /translation="MFSIFHIKQYKDFIQAILEDKGPLVTGEEAIKSLKLIKGIYKSS
                     RLGEKVELN"
BASE COUNT          654 a          388 c          219 g          616 t
ORIGIN      
        1 ccttatttaa aggagcagta accacccctt taattttccc ccgaagagcc cattcaattg
       61 ctttttcaac atatctatag gcagcatcgc cacaaagggc agatactttc ccaaattcaa
      121 aatcatctaa tctaatatcc aatacattaa ctaaatttat ggacgctgga tcaaattcat
      181 caatatcaga tattaaattt atcttttcat ccatatccaa tagttctaaa aaatgctcca
      241 ctaaatccct gctgccaaaa attatagatt tttccctaaa gtcttcattt tttaaagcct
      301 ttaaagtaat ttcacacccg atacctgccg gatctcccat tgtaattcca atataatcca
      361 tggtctgcct ccatttcttt tgaccaaata ccttttaact agcctgattt tataaaaaac
      421 attctatcac agccaattta taagctcaat agattattca actgcctctt tcctatcttt
      481 actaaagtaa ccactcagac ctactaaggc tccaattatt aaaaccatac caataattat
      541 cctaagatct atagtctcat ctaaaaatat ataccctaaa atagctccca taacaaccct
      601 actattggcc accatagacc ctgtggcgga atcaacatac ctataaccta gggtaataaa
      661 tatttgccct aaaactccac atataccaga taatagggca ggtaataggc cagccatttc
      721 aaaggtggca aaatccctta ataccagcgg caggttaact aatgtaccta ccagcattac
      781 ataaaatact attaaatagc tttcattatc cctgctggca atagttaagt acaagatact
      841 aaaggcccct actacagccg ataggaaggc aaaaaagtct ccaatattaa tatacccaaa
      901 acttggatta gcaactatat aagatcccaa cattattatg gttagataaa gataagagct
      961 ttttttaatc ttttctttag taaaataggg tgcaaaaagg actacaaaaa caggatatga
     1021 catatgtaac atattagtat tggttatggt ggtatattga aatcccaagg aaaaggtgat
     1081 catagcaaga ctattaaaga ttccccttaa gatcatgggt ttcatattat cggacttaaa
     1141 gcttttcccc ttaaacataa tataagcaaa cattaaaata gctcctatgg aaaacctaaa
     1201 aaaggccgaa agtataccag acatattagt tgtgctggta acaattttcc caaacataga
     1261 gctaagggta aaaaagaaag aagaaacaag tattagtaca atacccacaa ataacaactc
     1321 cttattttta aaaaataaca aatctctaat ataatgatat cacataactt attacagccc
     1381 tgtttccagg attaattaag ttctaccttt tctccaagcc tagaagattt ataaattcct
     1441 tttatcagct tcaagctttt aattgcctct tcaccagtaa ctaaaggacc tttatcttct
     1501 aaaatagctt gtataaaatc cttatactgt tttatatgga aaatactaaa caactttctc
     1561 ctttgtattg ctatgtaatt ttagttatct ctttcctgac ccaaattcct ctaatatact
     1621 taagtaaaac ctaatttcag tatctctgac aacttgcaaa agagatatgt tttctagcac
     1681 cttgctggct gctattcaca ttatttctct gcaccatttt atctttctag atctagaatt
     1741 ttaattttcc tttattcatt ctaccagttt ttaaaatgat ctatgtattt aaattagcat
     1801 atattgtgat tacttctact atttgttgta taatttcacc cataaagtca tccttcattt
     1861 gtaatcttta tttgttt
//
LOCUS       DATGSI010000317         1041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_165463, whole genome shotgun sequence.
ACCESSION   DATGSI010000317 DATGSI010000000
VERSION     DATGSI010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165463"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..323)
                     /locus_tag="VK031_04940"
     CDS             complement(72..323)
                     /locus_tag="VK031_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53297.1"
                     /translation="MRADEFLGIVKDNRKEVNFKLGVVADLFGNGTAKITFDGETEAS
                     TKQYSYLSSYKPKKGDRVLLAKVGGTYVILGNLMFKEIL"
     gene            complement(320..>1041)
                     /locus_tag="VK031_04945"
     CDS             complement(320..>1041)
                     /locus_tag="VK031_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53298.1"
                     /translation="DRFDKRYVIQEKSSYLKAITDILLEAGITKINIAETDLMLRTTK
                     EFEIGTEKLRVINELLQEINYTSLWVDENGYYTAKPYILPNEREIEYTYADDKLSIMY
                     PGIEEELDLFNVPNKFIVVASNPESSPLVSRYENINPKSLTSIPSRERVIVDYREVDD
                     IANQEALDEYTKRIAYEASQVFGYVNFNTTIMPFHSYSDLLEIKNSILGIEANYVETR
                     WRMKLKAGTQMEHTARRVIQI"
BASE COUNT          279 a          219 c          117 g          426 t
ORIGIN      
        1 aaatacccgt caactaccat gttttcaaat tctttatctt ctgtttctcc agtagttcct
       61 tgtactacat cttataatat ctccttaaac atcagatttc ctaatattac atatgttcca
      121 cccacttttg ccaaaagaac cctatcacct ttttttggtt tatagctaga taaatatgaa
      181 tattgttttg tgcttgcttc tgtttcccca tcaaacgtta ttttcgcagt accattacca
      241 aataaatcag ctactacacc taatttaaaa tttacttctt tcctattgtc tttaactata
      301 cctaaaaatt catcagccct catatttgta tcactctcct agctgtatgc tccatttgcg
      361 tccctgcttt taacttcatt ctccatcttg tttctacgta attggcttct atccctagaa
      421 tagaattctt tatttctagc aaatcagaat agctgtgaaa tggcattatt gtagtattaa
      481 aattcacata tccaaacact tggcttgctt cataggctat tctctttgtg tactcgtcta
      541 atgcttcttg attagctata tcgtcaacct ctctatagtc cactatcact ctttccctac
      601 ttggtataga tgttaaactc ttggggttta tattctcata tctcgatact aaaggagagc
      661 tttcaggatt gctcgctact actatgaatt tatttgggac attaaacaaa tctaattctt
      721 cctctattcc tggatacatt atagataatt tatcatctgc gtaagtgtat tctatttctc
      781 tttcatttgg caaaatataa ggttttgcag tataataacc attttcatct acccataggg
      841 aagtataatt tatttcttgc aaaagttcgt ttataactct taatttttct gttcctattt
      901 caaattcctt tgtagttctt aacattaaat ctgtttcggc tatatttatt ttagttattc
      961 ctgcttctaa caaaatatcc gttattgcct ttaagtagct tgatttctct tgtattacat
     1021 acctcttatc aaatctatcg t
//
LOCUS       DATGSI010000318         1892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_683547, whole genome shotgun sequence.
ACCESSION   DATGSI010000318 DATGSI010000000
VERSION     DATGSI010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683547"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VK031_04950"
     CDS             <1..398
                     /locus_tag="VK031_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HLS53299.1"
                     /translation="AFSAAAELGAPIMHDFASISLSDLLTPWQVITKRIEKAAEADFV
                     LAIYNPRSRGRKDYLKKAVGIIKKYRKKDTPVGIVRNSGRQERTITLTRLDEIDYEKV
                     DMLSIVLIGNSNSFVKGNQIVTPRGYEIK"
     gene            395..1120
                     /gene="cobK"
                     /locus_tag="VK031_04955"
     CDS             395..1120
                     /gene="cobK"
                     /locus_tag="VK031_04955"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HLS53300.1"
                     /translation="MIWIIGGTTEARQLVERLKGKDFLVTVATESGKEFIETKNLRVG
                     RMDLKQMDSFVKEEKIRVIVDMSHPYAKIVSKNARTVAQANKIEYIRYVREMKEYEDV
                     IELSSYEEAYAYLKSIKGTVFFTTGSKNIKDFEKVRGNNRFIYRVLPALESIEECKNN
                     NVKLRDIIAVLGPFSEEYNRVMFKEMGADYVIMKDSGKIGGTLDKIKACHRLGIKPIV
                     ISRKKEQGLNNLSDIEKILIEKY"
     gene            1130..>1892
                     /locus_tag="VK031_04960"
     CDS             1130..>1892
                     /locus_tag="VK031_04960"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HLS53301.1"
                     /translation="MELLNETLKSIEGPNKKVKEETKLIWDNLVKPLGSLGKLEELAI
                     KIAGITGKTKNQINKKAVVVMAADNGVLEEGVSASPTVFTTLLAEAMTKKITGVATLA
                     KYVDADIVTVDIGLDSDFRHEKVINKKIARGTKNFTKEAAMSYEEAVQAIEIGIEIGD
                     KLFKEGYDILGVGELGMGNTTTSAAILSVLSGIEVEKVCGVGAGVTKEQLEKKINTIK
                     KGLELHKPKKEDPIDLISKVGGFDIGGMCGLYLSAA"
BASE COUNT          704 a          269 c          449 g          470 t
ORIGIN      
        1 cagccttttc agctgcagct gaactaggtg cccctataat gcatgacttt gcctctattt
       61 cccttagtga cttgcttacc ccctggcagg taatcactaa aaggatagaa aaggctgcag
      121 aggcagattt tgtcctggcc atctataacc caagatccag gggcaggaag gattatctaa
      181 aaaaggcagt gggaataata aaaaaatata gaaagaagga cacaccagta ggtatagtga
      241 gaaattctgg caggcaggag agaactatta ccctaacaag gctggatgaa atagactatg
      301 aaaaggtgga catgctatct atagtcttaa ttggtaatag taatagtttt gtaaagggca
      361 accaaatagt cacacctagg ggatatgaga tcaaatgatt tggataattg gagggaccac
      421 tgaggctaga cagttggttg aaaggctgaa gggtaaagat tttttagtaa cagttgctac
      481 agaaagtgga aaagaattta tagaaaccaa gaacctcagg gttggacgga tggatcttaa
      541 gcagatggac agctttgtta aagaggagaa aataagggtg atagtagata tgtcccatcc
      601 ttatgcaaaa attgtttcca aaaatgcaag gactgttgcc caagccaaca agatagaata
      661 tattagatat gtaagggaaa tgaaagaata tgaagatgta attgaactat cttcctatga
      721 agaggcctat gcctatttaa aaagtattaa ggggacagta ttttttacta caggttccaa
      781 aaacataaag gactttgaaa aggtaagggg gaacaataga tttatataca gggtcttacc
      841 agctctagaa agcatagagg aatgtaaaaa taataatgtt aaactaaggg atataatagc
      901 agttttagga ccattttctg aagaatataa tagggttatg tttaaagaaa tgggggcaga
      961 ttatgttata atgaaagata gtggaaaaat aggaggtaca ctggataaaa ttaaggcctg
     1021 ccataggcta ggcataaagc caatagtaat ttctagaaaa aaggagcaag gcctaaataa
     1081 tttatcagat atagaaaaaa ttttaataga aaaatattag gaggatatta tggaattact
     1141 taatgaaact ctcaaatcaa ttgaaggacc aaataagaag gttaaggagg aaacaaagct
     1201 aatttgggac aacctggtaa agcccttagg aagtttaggg aagctggaag agctagcaat
     1261 taaaattgca ggtattacag gtaaaaccaa aaatcaaatc aacaagaagg ctgtagtagt
     1321 aatggcggca gacaacggag tcttagaaga aggagtcagt gcttctccca cagtatttac
     1381 caccctgctt gctgaggcca tgactaaaaa aattactggt gttgccacct tagccaaata
     1441 tgtggatgca gatatagtaa ctgtagatat aggattagat tctgacttta ggcatgaaaa
     1501 ggttataaac aaaaaaatag ctagaggaac caaaaacttt accaaggaag cagccatgag
     1561 ttatgaagaa gctgtacagg ccatagaaat tggtattgaa ataggggaca agctctttaa
     1621 agaaggctat gatatattag gtgttgggga attgggtatg ggcaatacca ccaccagtgc
     1681 tgctattttg tctgtcctat cgggcataga ggtagaaaag gtatgtggag ttggggctgg
     1741 agtaaccaag gaacagctgg aaaaaaagat taacacaatc aagaagggac tagaattaca
     1801 taaaccaaag aaagaggacc ctatagacct tatctcaaag gtaggtggct ttgatatagg
     1861 aggcatgtgt ggcttgtatc tttctgctgc ta
//
LOCUS       DATGSI010000319         4530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_400235, whole genome shotgun sequence.
ACCESSION   DATGSI010000319 DATGSI010000000
VERSION     DATGSI010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400235"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VK031_04965"
     CDS             <1..680
                     /locus_tag="VK031_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLS53302.1"
                     /translation="NYRKIVAVEALLRWTHPKYKNISPLFMIRIAEESGIINDIGEWV
                     LKEACKQNKSWQDKGLAHIKVSVNVSAMQFEQEDFVATVEEVLFETGMDPKYLELEIT
                     ETNIMDNVVEKMDKMKELKDLGISISIDDFGTGYSSLAYFTRFPIDTLKIDKSFISGM
                     LKDENSETIVKTIINMGKSINLKLVAEGVETEEQLERLEEMGCDKIQGYLISKPLESK
                     KIVELFN"
     gene            713..2428
                     /locus_tag="VK031_04970"
     CDS             713..2428
                     /locus_tag="VK031_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HLS53303.1"
                     /translation="MGKQKNKITSIRTKISTGFLLAIILMIGIAITAVVGFTRTEDIY
                     DDIFNKIVPGVIESKDIQIFILEKTVALEGYLSTGNEEFLENYDYYNSLEERNINRSQ
                     EYAISDAELEHVQNLRNLSVEYNSIVSDLLELYEEGLEEEIIIKMENQIHPAMDDMRQ
                     VAQELIGLKEAHVGQFRFNLLDIQNSAKGVMTIIGVAAILASFLIAYFVIRSIIRPIR
                     AMIEATKTIAEGDLTEEITVKTRDEINILADTFNGMAKNLRQMIGEIAQISESVVTAS
                     EELSASSEQSNASSEEISTIVGEVAKAINEEASAISQSNTLVHDVQGGADEISDKIQY
                     VNRSASATLKSAEEGLNSSQEAVERINRIRDTTNETSHIVLNLNDSSKRIEGIIDVIR
                     EISEQTNLLALNAAIEAARAGETGRGFAVVADEIRKLAEESAESTEEIGQIITDIQKQ
                     IDQVVSAMEESNKEVDVGVTVINKSSNEFSNIFKEIEEVSSEIGKITDIVREMVDKTN
                     LVTSNFEHISAISEENAASAQEVSASVKEQTEAMEEITNLAENLAIIGDELNRAIEIF
                     KINKE"
     gene            2569..4242
                     /locus_tag="VK031_04975"
     CDS             2569..4242
                     /locus_tag="VK031_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HLS53304.1"
                     /translation="MYNILIGGAAGQGIDTLGILIGKILCRKGYFIHSYKDYMSRIRG
                     GHNFTQIRFGTEKIYSHWPILDVIIALDKETIEFHRERLNDGGIILCDESVEAEDEKI
                     IQVPMTRFARELKNNRVAGTVAAGVLFKLIGESLDYVEEVFEEKWEKQTAESNLEAFK
                     KGYDFGEERFEKIEPTKKDQIYINSSDAIALGALAAGVGFFASYPMSPATSIMTKLSM
                     KQNDAKIVVEQVEDEISAINMALGASYAGVRAMTATSGGGFSLMVEALSLQGITEIPI
                     VVVNAQRPGPATGLPTRTEQADLNLALYAGHGEFPRMIIAVRNPEDAFYQTARALNIA
                     DKYQTQVIILTDQFLIDATETIKPYDFSRIKIERHLMTKEELGDEEYKRYKPTESGIS
                     PRIMPGKIEGAVVLVDSDEHNEYGHITESAEVRTSMMDKRMRKQEGLVSEIQEPDYFG
                     VDNPETLIIAWGSMYGPVREAVEMLNKEDISVGGLIFGDLYPLPTRLLKKYAETSKTL
                     VNVEMNFVGQLGRLIRQETGIVMDKSILNYNGRQLDSYTVYSRIKKEVI"
     gene            4243..>4530
                     /locus_tag="VK031_04980"
     CDS             4243..>4530
                     /locus_tag="VK031_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLS53305.1"
                     /translation="MLDIKMYDSKDEIAWCPGCGNFGILSALKETLLEMELLPHEVVI
                     VSGIGQAAKTPHYINTNGFNGLHGRAAPPAQGIKIANKDLKVIIHSGDGDSY"
BASE COUNT         1637 a          632 c         1053 g         1208 t
ORIGIN      
        1 taaattatag aaaaatagtg gcagttgagg ccctcctaag atggacgcat ccaaaatata
       61 aaaatatttc acccctattt atgattagaa tagcggaaga aagtggtata atcaacgaca
      121 ttggagaatg ggtgctgaaa gaggcttgca aacaaaataa aagttggcag gataagggcc
      181 tggcccacat taaggtgtct gtaaatgtat cagcaatgca atttgaacaa gaagattttg
      241 tagccacagt ggaagaggtt ttatttgaaa cgggtatgga tcctaagtat ttagaactag
      301 aaattacaga aacaaatatt atggataatg tggttgaaaa aatggataag atgaaagaat
      361 tgaaagactt gggaatttcc atatctatag atgattttgg gacaggctat tcttcactgg
      421 cctattttac tcgattcccc atagacacct taaagattga caagtccttt atcagcggta
      481 tgttaaagga tgaaaactca gaaacaatag taaaaacaat tattaatatg gggaaatcta
      541 ttaatttaaa actagtagca gaaggggtag aaactgagga acaattggag agattggaag
      601 aaatgggttg tgacaaaatt caaggctatt tgataagcaa gcctttagag tctaaaaaga
      661 tagtagaatt atttaactaa gggttttaaa gcataattat aggaggaagg caatgggaaa
      721 acagaagaat aaaattacct caataagaac aaaaatatct actggatttc ttttggccat
      781 tatactgatg ataggaatag ctattactgc agttgttggt tttaccagga cagaagatat
      841 ctatgacgat attttcaaca aaattgtacc aggtgttata gaaagtaagg atatacaaat
      901 atttatacta gaaaaaactg tggccctgga aggttatcta tctacaggta atgaggaatt
      961 tttggaaaac tatgattatt acaatagttt ggaagaaaga aatattaata ggagtcaaga
     1021 atatgcaatc agtgatgcag aactagagca tgtccaaaat ttaagaaatt tatcagttga
     1081 atataatagt attgtatcag acctacttga attatatgag gaaggattag aagaagaaat
     1141 cataataaaa atggaaaacc aaatccatcc tgcaatggat gatatgaggc aggtggccca
     1201 ggaattaata ggcctaaagg aggcccatgt aggtcaattt agatttaacc tattagatat
     1261 tcaaaatagt gctaagggtg ttatgaccat tataggagtt gcggctatcc ttgccagttt
     1321 tctaattgct tattttgtaa tcagaagtat tataaggcct attagggcta tgatagaagc
     1381 cactaaaacc atagccgaag gtgatcttac tgaagaaatt actgtaaaaa ccagggatga
     1441 aatcaatata ctggcagata cctttaatgg catggccaag aacttaaggc aaatgatagg
     1501 ggaaattgct caaatatccg aaagtgtggt tacagcttca gaggaactat cggcctcatc
     1561 agagcagtct aatgctagtt cagaggaaat atctactata gtaggtgagg tggccaaggc
     1621 cattaatgag gaggccagtg caatttcaca gtcgaatacc ctggttcatg atgtacaggg
     1681 aggggctgat gaaatatctg ataagattca gtatgttaat aggtctgcca gtgcaacctt
     1741 aaaatcagca gaggaaggac taaattcatc tcaggaagct gtggaaagaa tcaatagaat
     1801 tagagatact accaatgaga catctcatat agttttgaat ttaaatgata gttctaaaag
     1861 gatagaaggg attatagatg tcataaggga aatatctgag caaactaatc tgttggcgtt
     1921 aaatgcagcc atagaggcag caagggccgg tgaaacaggt agagggtttg cagtggtggc
     1981 agacgaaata agaaagctgg ctgaagagtc ggctgaatct acagaagaaa ttggccaaat
     2041 tataacagac attcaaaaac agattgatca ggtagtatca gccatggaag aaagtaataa
     2101 ggaagtagat gtgggagtta cagtaataaa caaatctagc aatgaattta gcaatatatt
     2161 taaggaaata gaggaagtat catctgaaat tggaaagata acagatattg ttagagaaat
     2221 ggtggataag actaatttag ttacatctaa ctttgaacat atctccgcca tatctgaaga
     2281 aaatgctgca tcagctcaag aggtgtctgc cagcgttaaa gagcagacag aggccatgga
     2341 agagataaca aacctggcag agaatttagc cataatagga gatgagctaa accgggccat
     2401 agaaatattt aagattaaca aggaataatt taatataaga aagatagaaa gactatggat
     2461 ttgtccatag tctttttttc aacaaaaaat gaaaaaattg tagatatatg tctagattta
     2521 taaatttagg taaagctaat aaagataaat ttatttgggg gtgtgtccat gtacaatata
     2581 ttaataggcg gtgcagcagg ccaaggaata gatactttag gaatcttgat tgggaaaatc
     2641 ctatgcagga agggatattt tatccattct tataaagatt atatgtctag gattcgaggg
     2701 ggacataatt ttacccaaat cagatttgga acagagaaaa tttattccca ttggcctata
     2761 ttagatgtaa ttatagcgct agataaggaa accatagaat ttcacaggga aaggctaaac
     2821 gatggaggaa tcattttatg tgatgagtct gttgaggctg aagatgaaaa aataattcag
     2881 gtgcctatga caagatttgc ccgagaacta aaaaacaacc gggttgcagg aacagtggca
     2941 gctggagttt tatttaagct gataggggaa tcactggatt atgtagaaga ggtgtttgaa
     3001 gaaaagtggg aaaagcaaac tgcagaatcc aatctggagg cttttaaaaa gggctatgat
     3061 tttggggaag aaaggtttga aaagatagaa ccaaccaaaa aggaccaaat atatatcaat
     3121 tcaagtgatg ccatagcctt gggagccttg gctgcaggtg taggcttttt tgcctcttac
     3181 ccaatgtctc ctgcaactag tattatgact aaattatcca tgaaacaaaa tgatgctaag
     3241 atagttgtag aacaggtgga agatgagatt tctgctataa atatggcttt gggagcctct
     3301 tatgctgggg taagagctat gacagccacc tccggtggag gtttttcctt aatggtggag
     3361 gccttaagtt tacagggaat aactgagata cctatagttg tggtaaacgc tcaaagacca
     3421 gggccagcca caggcctacc tactaggact gaacaggcag atttaaacct tgccttgtat
     3481 gcaggccacg gtgagtttcc gaggatgata atagcagtta gaaatccaga agatgccttc
     3541 taccaaactg ctagagcctt aaatatagcg gataagtatc aaactcaagt gataatatta
     3601 acagatcaat ttttaataga tgctacagaa accatcaaac cctatgattt tagccggata
     3661 aagattgaaa gacatcttat gactaaagaa gaattgggag atgaggaata taaaaggtat
     3721 aagcctacag aaagtgggat ttctccaagg ataatgcctg gaaaaataga gggagcagta
     3781 gtgctggtag atagtgatga gcacaatgaa tatggccaca ttacagaatc tgctgaagtt
     3841 agaacatcca tgatggataa gaggatgaga aagcaggagg ggctggtaag tgaaattcag
     3901 gagccagatt attttggggt agataatcct gaaaccctaa taatagcttg gggctccatg
     3961 tatggaccag tgagggaggc tgtggaaatg ctgaataagg aggatatatc tgtagggggc
     4021 ttaatatttg gagatttata tcctttacct accaggcttt tgaaaaagta tgctgaaaca
     4081 tctaaaactt tggttaatgt agaaatgaat tttgtagggc aattgggcag gttgatcagg
     4141 caggagacag ggattgttat ggataaaagt atcttaaatt ataatgggcg acaattagac
     4201 tcctacactg tttattcaag gattaaaaag gaagtgatat aaatgttgga tataaaaatg
     4261 tatgattcca aggacgaaat tgcctggtgt ccaggatgtg gaaactttgg gattttatca
     4321 gccttaaagg aaactttatt ggaaatggag ttactacccc atgaagttgt tatagtatcc
     4381 gggatagggc aggctgctaa aactcctcac tatataaata caaatgggtt taatgggctt
     4441 catggaaggg cagctccccc ggctcagggg ataaagatag ccaataagga tctaaaggta
     4501 attatccact caggagatgg tgattcctat
//
LOCUS       DATGSI010000320         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_213242, whole genome shotgun sequence.
ACCESSION   DATGSI010000320 DATGSI010000000
VERSION     DATGSI010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213242"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VK031_04985"
     CDS             complement(<1..569)
                     /locus_tag="VK031_04985"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HLS53306.1"
                     /translation="MDQVEKEIYNLGYEKIAFIDEVGRGCLLGDVLACAIILPKGLRI
                     EGVKDSKKLSEKKRNLLYNIILDKSLAFGIGRVDAATIDKINIKESTRLAMKRAVEDL
                     KDKSGRKVKPDFLLIDAEKIDKEIPQKSIIKGDDLSHGIACASIIAKVYRDRLCLKWD
                     KEYPGYNIGKNKGYGTKEHREKIKELGITP"
     gene            complement(571..1425)
                     /gene="ylqF"
                     /locus_tag="VK031_04990"
     CDS             complement(571..1425)
                     /gene="ylqF"
                     /locus_tag="VK031_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HLS53307.1"
                     /translation="MNINWYPGHMKKTRETIAKSLSLVDIVFELIDARIPYSSRNPVI
                     DDIVKNKPRILILNKMDLADPKFNKIWKDHFQEQNITTILFNSLDKKNIRTLIDTANS
                     MVEKNRQTYKQKGIINKPVRAMILGIPNVGKSTLINNLSGRKGARTGNVPGVTRGNQW
                     IKTRSNMELLDTPGILWPKFEDKDVGLSLAFIGSIKDDLLDMETLALRLIERLMIKED
                     KKLEERYKISVSNKTALQVMEDIALKRGCIIRGGEIDYTKVSNIILDEFRKGTIGRIT
                     LELPETGE"
     gene            complement(1472..1804)
                     /gene="rplS"
                     /locus_tag="VK031_04995"
     CDS             complement(1472..1804)
                     /gene="rplS"
                     /locus_tag="VK031_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HLS53308.1"
                     /translation="MIEAEQLRNDIPDFNVGDTVQVHYRVVEGTRERIQVFEGTVIKR
                     QGGGIRETFTVRRLSYGVGVERTFPVHSPRIDKLVVTRKGKVKRSKLYYLRERQGKSA
                     KIKERRDF"
     gene            complement(1917..2648)
                     /gene="trmD"
                     /locus_tag="VK031_05000"
     CDS             complement(1917..2648)
                     /gene="trmD"
                     /locus_tag="VK031_05000"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HLS53309.1"
                     /translation="MKIDVLTLFPEFFSLFSHWSIIGRAVDNGIVDINYTNIRDFSKN
                     KHRKVDDYPYGGGPGMVMTPQPIYDAIEKVRDKDSKVIYLSPQGRKLNQEILINLSKE
                     THLILLCGHYEGIDNRIIEHYIDEEISIGDYVLTGGEIPAMVIMDGIIRLLPGVLTSE
                     EAYEDESHYHGLLEYPQYTRPRQFKEHKVPDILLSGNHGKIKKWRKYQSLKATILKRP
                     DLLEKIDKDEKLKELIAKIQAEINS"
     gene            complement(2645..3148)
                     /gene="rimM"
                     /locus_tag="VK031_05005"
     CDS             complement(2645..3148)
                     /gene="rimM"
                     /locus_tag="VK031_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587774.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HLS53310.1"
                     /translation="MNHIAVGEIINTHGISGEVKVLPLTHDVERFSELARVYIGKDKK
                     KANISNVKYHKGLVIIEFEEFDNINLVLPFKGQYLYIDEADRIKLPEDHFFIYEIIDC
                     QVVDLSGNKIGYIKDVLEYASNDVYVVSDYSKEKEYLIPAVKEFIKEVNILEKLVVID
                     PIEGMIE"
     gene            complement(3196..3426)
                     /locus_tag="VK031_05010"
     CDS             complement(3196..3426)
                     /locus_tag="VK031_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HLS53311.1"
                     /translation="MGELVEYIAKALVDNPDEVEVNEVEGSQSIIIELKVAQEDMGKV
                     IGKQGRIAKAIRTVVKAAAIKENKRVVVEIIQ"
     gene            complement(3448..3723)
                     /gene="rpsP"
                     /locus_tag="VK031_05015"
     CDS             complement(3448..3723)
                     /gene="rpsP"
                     /locus_tag="VK031_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HLS53312.1"
                     /translation="MAVKIRLRRMGAKKNPFYRIIVADSRAPRDGRFIEEIGYYDPLT
                     EPKTVKIDNEKAMAWLNNGAKPTDTVNRLFRENGVYNQLEDNEANEE"
     gene            complement(3764..>4684)
                     /locus_tag="VK031_05020"
     CDS             complement(3764..>4684)
                     /locus_tag="VK031_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HLS53313.1"
                     /translation="RPAAIKQLEVVGEKVDVPVFSMGSKNNPIDIAKAALEHGKRHGN
                     DVVIIDTAGRLHIDEELMEEIEGINQAVTADEVLLVLDAMTGQDAVNVAQSFDDRLGI
                     SGVVLTKLDGDARGGAALSIRAVTDKPIKFVGVGEKFDQLEAFHPDRMASRILGMGDV
                     LSLIEKAQESIDAEKAMELEKRIRSAQFTFDDFLDQLEQMKNLGPLDQLLGMLPGMNS
                     KALKGLKVDEKDLARIEAIIKSMTKKERQNPEIINSSRRKRIAAGSGTSVPEVNKLLR
                     QFRETKKMMKRFSDMEKTMKRKGRMGFPFF"
BASE COUNT         1264 a         1036 c          606 g         1778 t
ORIGIN      
        1 ggggttatgc ctaattcctt tattttctcc ctatgttcct ttgttccata acctttgttt
       61 tttccaatat tgtagcctgg gtattcctta tcccacttta aacatagcct gtccctgtag
      121 accttagcaa taattgaggc acatgctata ccatgggata aatcatcacc ttttattata
      181 cttttttgag gtatttcctt atctatcttt tcagcatcaa taagaaggaa atcaggcttt
      241 acctttctac ctgatttatc ctttaagtcc tccactgccc ttttcatagc caaacgagta
      301 ctttccttta tatttatttt gtcaatggta gctgcatcta ccctacctat tccaaaggcc
      361 aaggacttat ctagtataat attatataaa agatttctct ttttttcact aagtttttta
      421 gaatccttaa cgccctctat acgtaatcct tttggaagga ttatggcaca ggctaaaaca
      481 tcacctagca ggcaacccct tcctacttca tcaataaatg ctattttttc atagcctaag
      541 ttataaattt ctttttccac ttggtccaac ttattcacct gtttctggta attctaaagt
      601 aattcttcca atagttcctt ttctaaattc atccaggatt atattactga ctttagtata
      661 atctatctct ccccctctaa taatacatcc tctttttagg gctatgtcct ccataacctg
      721 gagggctgtt ttattagaga cagaaatttt atacctttct tctaactttt tatcttcctt
      781 tatcattaat ctttctatta gccttagagc caatgtttcc atatctagta agtcatcctt
      841 aatggaacca ataaaagcta agctaaggcc tacatcctta tcttcaaatt ttggccataa
      901 aatcccaggt gtatctaaaa gttccatgtt ggatcttgtt ttaatccatt gatttcctct
      961 tgtaacacct ggaacatttc cggttctagc tcccttcctg ccagacaagt tattaataag
     1021 ggtagattta cccacattag gaatacctag gatcatggct ctaacaggct tattaattat
     1081 tcccttctgc ttataggtct gcctattttt ttcaaccata ctattggctg tatctattaa
     1141 ggttctaatg tttttcttat ctagtgaatt aaataatatg gtagtaatat tttgctcttg
     1201 gaagtgatct ttccagattt tattaaattt aggatcggcc agatccatct tgtttaatat
     1261 aagaattctt ggcttgtttt tcacaatatc gtctataaca gggtttctac tgctataggg
     1321 aattctagca tcaattaatt caaatacaat atctactaga gataagcttt tagctatggt
     1381 ctccctagtt tttttcatat gtcctggata ccagtttata ttcattaaat caccaccata
     1441 tataaaaagg gactctataa gtcccattaa attaaaaatc tcttctttct ttaattttag
     1501 ctgatttacc ttgtctttct cttaaataat acaacttgga tcttttaacc ttaccttttc
     1561 tggttacaac cagcttatct atccttggtg aatgtacagg gaaggttctt tccactccaa
     1621 caccgtatga taatcttctt actgtgaaag tttctctaat gcctccacct tgccttttga
     1681 ttactgtacc ttcaaatact tggatccttt ctctagttcc ttctacaact ctgtaatgaa
     1741 cttgaacagt atctcccaca ttaaaatcag gaatatcatt tcttaattgt tctgcttcta
     1801 tcatttttat tatactcact gttcaaaacc tcccttctcc ttagatgttc ataccctttg
     1861 gtagaggacc atccgtaatg catacctagg tattataaca tatgggatta gtcaattcaa
     1921 ctatttattt ctgcttgaat ttttgcaatt aattctttta atttttcatc tttatctatc
     1981 ttctctaata aatctggtct tttcaaaata gttgccttta gtgattggta tttacgccat
     2041 tttttaattt taccatgatt tccagacagt aatatatccg gaactttatg ttccttaaat
     2101 tgacgaggtc tagtatattg ggggtattcc aacaaaccat ggtagtggga ctcatcttcg
     2161 taggcttcct cactagttaa aactcctggc aggagcctaa ttataccatc cataataacc
     2221 atggctggaa tttctccacc tgttagcaca taatcaccta ttgaaatctc ttcatctata
     2281 tagtgttcta taattctatt atctattcct tcatagtgac cgcataaaag aattagatga
     2341 gtttctttag atagatttat tagtatttcc tgattaagtt tccttccctg aggtgataaa
     2401 tatataacct tagaatcttt atcccttacc ttttctatgg catcataaat aggttgtgga
     2461 gtcattacca ttcctgggcc tcccccatat gggtaatcat caacttttct atgtttattc
     2521 ttagaaaagt ccctaatgtt tgtatagttt atatctacta tgccattatc tacagccctt
     2581 cctataatac tccaatggga aaataaggaa aagaattcag gaaacagtgt taatacatct
     2641 atcttcattc tatcattcct tcaatagggt ctataacaac tagcttttcc aatatattaa
     2701 cctctttgat aaattccttg actgctggaa tcaaatactc tttctccttt gaataatcac
     2761 taaccacata tacatcattg ctggcatatt ctaatacatc ctttatatag cctatcttat
     2821 tgcctgataa atctaccact tgacaatcaa ttatttcata tatgaagaag tgatcctcag
     2881 gcaattttat tctatcagcc tcgtctatat agaggtattg gcccttaaaa ggaagcacaa
     2941 gattaatatt atcaaattct tcaaattcta taattactaa gcccttgtgg tatttgacat
     3001 ttgaaatgtt ggctttcttt ttatctttac caatgtatac ccttgccagt tctgaaaatc
     3061 tttccacatc atgggtaagg gggagaacct ttacttctcc cgatatgccg tgtgtattga
     3121 ttatttcccc tactgcaata tgattcatat ctacaccctc taatagagtc cttaagggat
     3181 tagaaagcta atcccttatt gtataatttc aaccactact cttttgtttt ccttaattgc
     3241 tgcagcctta actactgtcc taatggcttt agcaattcta ccttgctttc caattacctt
     3301 tcccatatcc tcttgagcaa cctttaattc tataattata gattgggaac cttccacctc
     3361 attgacttct acttcatcag gattatctac cagtgccttt gcgatgtatt ctactaattc
     3421 acccattgtt acacctccta gaattaatta ctcctcattg gcctcattat cctctagttg
     3481 attatatact ccattttccc taaatagcct attaacagtg tcagtcggtt tagctccgtt
     3541 atttaaccaa gccattgcct tctcattatc tatcttaact gttttaggtt cagttaatgg
     3601 atcataatag cctatctctt cgatgaatct accatctctt ggtgctcgag aatcagctac
     3661 aattattcta taaaaaggat ttttcttagc acccattctt cttaatctaa tctttacagc
     3721 catttttttc acctccttta aaagtgttgg aataattatt tatttagaaa aagggaaatc
     3781 ccattctccc ttttcttttc attgtttttt ccatgtcaga gaatcttttc atcattttct
     3841 ttgtctctct aaattgtctt aaaagcttat ttacttcagg tactgaagtt ccactacctg
     3901 cagctattct tttacgtcta ctactattta ttatctcagg attttgtctt tcctttttag
     3961 tcatagactt aataatagcc tcgattctag ctagatcttt ttcatctacc tttagcccct
     4021 ttaatgcttt tgaattcatt cctggtagca taccaagtag ttgatctagt ggccctagat
     4081 ttttcatctg ttccagttgg tctaaaaaat cgtcaaaagt aaactgggca gatctaattc
     4141 ttttttctag ctccatagcc ttctctgcat ctatgctttc ctgtgctttt tcaattaggc
     4201 ttaaaacatc acccattccc aggattcttg atgccattct atcaggatgg aaggcctcca
     4261 gttggtcaaa cttttccccc acacctacaa atttaatagg cttatctgtt acagctctaa
     4321 tagataaagc tgcccctcct ctagcatcac catctaattt ggtcagcaca acacctgaaa
     4381 tccctaatct atcatcaaag gattgggcca cattaacagc atcctgacca gtcattgcat
     4441 ctaggactag taaaacttca tcagcagtta cagcctgatt aattccttct atttcctcca
     4501 ttaactcctc gtcaatgtgt agtctacctg cagtatctat aattacaaca tcattaccat
     4561 gcctcttacc atgttccaag gctgccttag caatatctat ggggttgttt ttacttccca
     4621 tggaaaacac tggtacatca actttttccc ctactacctc caattgctta atggccgccg
     4681 gtct
//
LOCUS       DATGSI010000321         1573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_591216, whole genome shotgun sequence.
ACCESSION   DATGSI010000321 DATGSI010000000
VERSION     DATGSI010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591216"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VK031_05025"
     CDS             complement(<1..1461)
                     /locus_tag="VK031_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53314.1"
                     /translation="MKRFKQIISFILIVLLLSSLLAPVLAEKEGGRTIHIKTKEDLYN
                     FAKKASFDKYSLGLTVILENDIDLKGENFTPIPIFKGTFDGRGHTIKGVKLEVEGSNQ
                     GFFRYLEEEGVIKNLTIEGSIMPKGDQKNIGGLVGFNKGTIDKAHFKGILRGKNTIGG
                     IAGYNSTSGKIINSSSDSFIYGERKVGGIAGVNAGVILRGHNKGHINTTVEETSIDLQ
                     ELVDEELSYSKIFTDATDIGGIVGANTGVIKGAENHGTIGYPSVGYNIGGIAGRQSGY
                     IDSCTNYGKVLGRKDVAGVVGQIEPNVKMTIPPNRLTDLNNELNKLQDIINKLNNNFK
                     ISSQAIGKDLKSVQEDMDKARGHIKVLIDYTEGIIDEGLEGIDEINILVVEILDRLIP
                     INESLENILDEIMEAIGPLDQLIKDLVDSLENRPDIREDMEELGELLSQLGGHTSSLR
                     KGLADLQDKLGKAIDMIQAGNIIGALELINSMDTASV"
BASE COUNT          403 a          380 c          207 g          583 t
ORIGIN      
        1 tacactggcc gtatccatag agtttataag ctccaaggcc cctataatat tgcctgcctg
       61 tatcatgtct atggcttttc ccaatttgtc ctgtaaatct gccaggccct ttcttaagct
      121 ggaagtatgt ccccctagtt gggataaaag ttctcccaat tcttccatgt cctcccttat
      181 atcaggcctg ttttccaatg aatctacaag atcctttata agctgatcca agggcccaat
      241 agcctccatt atttcatcta aaatattttc taaggactca ttaataggaa taagcctatc
      301 cagtatttct accaccagta tatttatttc atctatgccc tccaagcctt catcaattat
      361 accttcagta taatctatta aaactttaat atgacctcta gccttatcca tatcctcttg
      421 gactgacttt aaatcttttc ctatggcttg ggaacttatt ttaaagttat tgtttaactt
      481 attgattata tcttgtaact tatttaattc attatttaaa tctgtcaacc tgttaggtgg
      541 tatagtcatt ttcacatttg gctctatctg tcctacaaca cctgccacat cttttctacc
      601 taataccttt ccataattgg tacaactatc tatatagcca ctttgcctac ctgctatacc
      661 tcctatattg taaccaacac tgggataacc aatagtacca tggttttcag cccccttaat
      721 tactcctgta ttggcaccaa caattcctcc tatatctgtg gcatcggtaa atattttgga
      781 ataacttaac tcttcatcta ccagctcctg gagatcaata cttgtttcct caacagttgt
      841 atttatatgg cctttattat ggcccctaag aatcactcct gcatttactc ctgcaattcc
      901 tccaaccttt ctttcaccat aaatgaagct gtcagagcta gaatttatta tttttccact
      961 ggtactattg taacctgcta ttccgcctat agtatttttc ccacgtagta ttcccttaaa
     1021 gtgggcctta tctatggttc ctttattaaa tcctactaga ccacctatgt ttttttgatc
     1081 acctttgggc attatgctgc cttctattgt taggttttta ataacaccct cttcctccaa
     1141 gtaacggaaa aatccttggt tagaaccctc cacctctagc ttgactccct taattgtatg
     1201 gcccctgcca tcgaaagtgc ccttaaatat tgggattgga gtaaaatttt ctcctttaag
     1261 atctatatca ttttctaaaa ttacagttaa gccaagggaa tatttatcaa aggaggcctt
     1321 tttcgcaaag ttatataggt cttcttttgt ctttatatgg atagtccttc cgccttcttt
     1381 ttctgccaat actggtgcca gtaagctgga taaaagtaaa actatcagta taaaggatat
     1441 aatctgctta aatcttttca tgctctaagt cctcctttcc tttagttgga tccacttctt
     1501 cctcccaatt ttccctgctt ggctttattt ctccaataac cttaccctta attgtagcat
     1561 ctatttctcc ctt
//
LOCUS       DATGSI010000322         1980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_119081, whole genome shotgun sequence.
ACCESSION   DATGSI010000322 DATGSI010000000
VERSION     DATGSI010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119081"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..423)
                     /locus_tag="VK031_05030"
     CDS             complement(163..423)
                     /locus_tag="VK031_05030"
                     /inference="COORDINATES: protein motif:HMM:NF022165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 family protein"
                     /protein_id="HLS53315.1"
                     /translation="MKSIIEGLGRLFLKLKLKLVGRDDKKDDRDVILKSLKEAEKEWK
                     TKEEYFNHATDPDLVDFAIYDIEASRRKYSYLLKKLKKEKNV"
     gene            complement(493..1299)
                     /gene="zupT"
                     /locus_tag="VK031_05035"
     CDS             complement(493..1299)
                     /gene="zupT"
                     /locus_tag="VK031_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HLS53316.1"
                     /translation="MNFSNFFLSFVLTVIAGLSMASGSLLSFLFKKDNKKFFTISLSF
                     SAGIMIYLSFMGLLPEGIAMIEDYLSEKSYIIGLISFFAGIIGIALIERLIHRIESMD
                     KHSDKTEDDTNGQHLGRLGIASAVAIAIHNLPEGLALFLAGLKDIGLATSLALAIIIH
                     NIPLSIAISTPIYYSTKSKKKAFTYTLLVGLCQPVGALLGYLVFHNFFNDLIFGIIFA
                     VIAGIMIFISLDELLPMAQKYDDHHIAIYGAIGGMLVMAISLVVFGHHQH"
     gene            1446..1521
                     /locus_tag="VK031_05040"
     tRNA            1446..1521
                     /locus_tag="VK031_05040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1479..1481,aa:Val,seq:gac)
     gene            complement(1525..>1980)
                     /locus_tag="VK031_05045"
     CDS             complement(1525..>1980)
                     /locus_tag="VK031_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HLS53317.1"
                     /translation="TSTGGTDIVAKVINKFFHLEIGKSLFLADFLIVLSAIYVFGIEL
                     GLYALLGIVINSAVIDNLITGFNTKIKVTIISKKEKQICKYITEKLDRGVTLFYGSGG
                     FSNKDMRIINTVVSRREYIRIKDYVKEIDPQAFIWVNIVHEVLGEGFSK"
BASE COUNT          667 a          379 c          272 g          662 t
ORIGIN      
        1 ttattagaaa ttttactagt aatctcattg ggaccactaa taataggcct aatacataaa
       61 gtagaactaa tcctaacaaa tacgcaaaaa cagttcctaa tgacataagt ccgaccccct
      121 acatagcatt atactataat ataggacaag ggacaaaaaa tattacacat tcttttcttt
      181 tttcagtttc tttaatagat aggaatattt tcttctagac gcttctatat catatatggc
      241 aaaatccacc aggtctggat cggtagcatg gttaaaatat tcttccttcg tcttccattc
      301 cttttcagct tcctttaagg attttaaaat aacatccctg tcatcttttt tatcatccct
      361 acctaccagc ttcaacttta gttttaaaaa taacctacct aatccttcaa taatagattt
      421 catataggcc acctccactt ccttatttaa atcattgaca aaaggattgg agaatatact
      481 ttagcctatt ctttagtgct ggtgatgacc aaatactact aagcttatgg ccattaccaa
      541 cattcctcca atagctccat atatggctat gtgatgatca tcatattttt gggccatggg
      601 caataactcg tccaaagaga taaatatcat aattccggct attacagcaa atataatgcc
      661 aaaaattaaa tcattaaaaa agttatgaaa aaccagataa cccagtaggg ctcctactgg
      721 ttgacaaaga cctaccaata aggtataagt aaaagctttt ttcttacttt tagttgaata
      781 atatataggt gtggagatag cgatgcttaa aggtatatta tgaattataa ttgccaaggc
      841 tagtgacgta gccaaaccta tatccttaag acctgcaaga aacaaggcta gaccttcggg
      901 taaattatga atagctatag ctacagcaga agctatccct aacctaccta aatgctggcc
      961 attggtatcg tcttctgttt tatctgaatg tttgtccatg ctttcaatcc tatgtattag
     1021 tctctcaatt aatgcaatgc ctataattcc agcaaaaaaa gaaatcaaac caattatata
     1081 gcttttttca cttagataat cctcaatcat tgcaatccct tctggcaata aacccataaa
     1141 ggaaagataa atcattatac cagctgaaaa acttagagaa atggtgaaaa atttcttatt
     1201 atccttctta aataagaaag ataataggct acctgaagcc atagataaac ctgcaattac
     1261 tgttaaaaca aatgataaaa agaaatttga aaaattcata aaatatcacc ctatctaaaa
     1321 tttatggaaa ttcttagcac ctaacagtat taccctttta taaggaattt aatctatgcc
     1381 cccttcagat aggttgatta ttattgtaat ttctattatt aaatgatata atataattaa
     1441 tcttagggcg gttaactcag ctggtagagt gtcatcttga cgtggtgaaa gccgtaggtt
     1501 cgagtcctat accgcccacc atttctattt tgaaaatcct tcccccaata cctcatgtac
     1561 tatattcacc caaatgaaag cttgcgggtc tatttctttt acataatctt taattcttat
     1621 gtattctctc ctagatacta ctgtgttaat tattctcata tctttatttg agaaaccacc
     1681 ggacccataa aatagggtaa ctcccctatc taatttttca gttatatatt tacatatttg
     1741 tttctctttt ttgcttatga tggttacctt tatttttgtg ttgaagccag taattaaatt
     1801 gtctattaca gcagaattta ttactatgcc taatagggca tagaggccta gttctatccc
     1861 aaatacgtag attgcagata aaactattaa aaaatctgct aagaagagag acttacctat
     1921 ttccaaatga aaaaatttat ttatcacctt agctactata tcggtgccac ctgtggatgt
//
LOCUS       DATGSI010000323         1249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_303952, whole genome shotgun sequence.
ACCESSION   DATGSI010000323 DATGSI010000000
VERSION     DATGSI010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303952"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VK031_05050"
     CDS             <1..427
                     /locus_tag="VK031_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HLS53318.1"
                     /translation="TRHIDKLETELLLLMEEEASLEKGFRDLEVVKRNYEGQLKDKEK
                     EYERLQTLIMQKIERKAKQIYEIENKIETGILEQYKKIRQQKLAGIVPVTDYVCGGCH
                     MTIPTYLGKALKMGKEIILCDYCGRILYYVKEELDSKHD"
     gene            551..896
                     /gene="rnpB"
                     /locus_tag="VK031_05055"
     ncRNA           551..896
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VK031_05055"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1095..>1249)
                     /locus_tag="VK031_05060"
     CDS             complement(1095..>1249)
                     /locus_tag="VK031_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HLS53319.1"
                     /translation="IYQNKFETLEELELQLAEYIYWYNHIRIHGSLGYISPVEYRYRE
                     NLELVA"
BASE COUNT          451 a          189 c          254 g          355 t
ORIGIN      
        1 tactaggcat atagataagt tggaaacaga acttcttctt ctaatggagg aggaagcgag
       61 cttggagaag ggatttagag acttggaagt ggtaaaaaga aattatgaag gtcaactaaa
      121 agataaagaa aaagagtatg aaagattgca gaccttaatt atgcaaaaaa tagagagaaa
      181 agccaagcaa atatatgaaa ttgaaaataa aatcgaaact gggatattgg agcaatacaa
      241 aaaaataagg cagcaaaaat tagcaggtat agttccagta accgactatg tatgtggagg
      301 gtgccatatg accataccta cttatctagg taaagccttg aaaatgggaa aagaaattat
      361 cctatgtgac tattgtggca ggatattata ttatgtaaag gaagaattag atagtaaaca
      421 tgattaaatc tttgaaatat ctagccataa ctgtaatctc tcagctgata atataaaagt
      481 acaaatttgt ccaatggtga aaattggttt tgcaatgaag caaaaaagat gatatattat
      541 agatagactt ggagtaagtt gtttggtcgc gtgctttaag catgaggaaa gtccgtgctc
      601 catagagcag gatgctggat aacgtccagt gggggcgacc ctaaggatag tgcaacagaa
      661 agataccgcc ttatactttg gtataaggta agggtggaaa ggtgaggtaa gagctcacca
      721 gcatttaggc gactaaatgg ctctgcaaac cccatccgga gcaagaccaa aaaggagtaa
      781 aagggatagc tgctcgctag ccttccaaat ggtaggtcgc atgaacctat tggcaacgat
      841 aggtcttaga ttgatgacca agtaaacaga acacggctta tagacttact cttcaaaaat
      901 atcttaaaat acagtcttat atggactgta ttttttgctt atgtcctgct tattgcatgt
      961 agtgaaagtg agcctttaag ccactttaaa tactattact atttatttgg aacttaaata
     1021 gatgtaaatt tttgaaaggc aacccctttt tagacaaatt cactccgaac aagaatatac
     1081 tttctcaata tcacttatgc tactaattct aagttctctc tatatctata ttctactggt
     1141 gaaatatagc ccaaggagcc atgaatcctt atatgattat accagtaaat atattctgct
     1201 aattgtaatt ctagttcctc tagggtctca aatttatttt gatatataa
//
LOCUS       DATGSI010000324         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_119123, whole genome shotgun sequence.
ACCESSION   DATGSI010000324 DATGSI010000000
VERSION     DATGSI010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119123"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VK031_05065"
     CDS             complement(<1..173)
                     /locus_tag="VK031_05065"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HLS53320.1"
                     /translation="MIDQASKLRTLFENKNKDTLIKKNQPMKIYSVVSGKGGVGKTNF
                     SVNLAIKLQQAGK"
     gene            complement(166..1167)
                     /locus_tag="VK031_05070"
     CDS             complement(166..1167)
                     /locus_tag="VK031_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53321.1"
                     /translation="MIIKTYIVNDMKEALIRASYELGKEAVIISQRPIRVGKWYNPFK
                     KKKLEVTVAVESNRKKQPAEKVVVNNETRYSVHNLIEKNPVFKNISDTTKSQLLGYCK
                     LHLKEDEYLTTEEIKNFMKMAFKDNGFEKKVRTRKVNVFVGPTGVGKTTTIAKIAARE
                     SLEKKRSVGLITIDTYRIGAVEQLNTYAEILNVPCEVVQEPKDMQATIRKLRDCDTIL
                     IDTLGTSQRNKEKLDDIKKYLDNITNKTNIHLVMSISTDRETTESILENYKKLNYNAL
                     ILTKLDEISSFSNLWNIVENYSYPIEYFTHGQDVPDDIKRATLDSLIDYSEEIYQDD"
     gene            complement(1164..>2312)
                     /locus_tag="VK031_05075"
     CDS             complement(1164..>2312)
                     /locus_tag="VK031_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HLS53322.1"
                     /translation="GLVLGSISYLLMENEKAELALEMAEAEASVSPVKEREEGVMTFQ
                     VEPIALEIGYGIIPLVDEGNHDNLINHISSIRKQCANEMGIIVNPIRIRDNLQLGANE
                     YVIKIRGNEIGRGEIHLNKYLVIDPGVEDFDIEGIPTKEPAFGLDAIWVDEKDREKID
                     LLGYTIVDPATVLVTHLKEVIRNHSHELLGRQEVQQLLEGIKERYNVVVDELIPDILS
                     LGEVQKVLQNLLKEDIPINDLVTILEVLADNGTKTKDIEMLTEYVRHALNRTIVNKYV
                     NDKGVLTVITIHPDLEELIANNIQKTMSGSIPVLKPDIITRIFDNINDLNNALLSQGI
                     QGVILSSPNIRVAFRNLISFNFPGLPVLSLNDVPNDVEIEAVGMVDKV"
BASE COUNT          607 a          491 c          325 g          889 t
ORIGIN      
        1 cttttccctg cctgctgtaa ttttatggcc agattaacag aaaaattggt cttacctacc
       61 ccgcctttac cactgaccac tgagtatatt ttcataggct ggttcttttt tattaaggta
      121 tccttatttt tattttcaaa cagtgtcctt aatttgctag cctgatcaat catcctgata
      181 tatctcctca ctataatcta tcaaactatc taaagtagcc ctctttatgt catctggcac
      241 atcttgtcca tgggtaaagt attctatagg ataggaataa ttctccacaa tattccataa
      301 gtttgaaaag ctacttattt catctaattt ggtcaatatt agggcattat aatttaattt
      361 cttatagttt tctaagatag attctgtagt ctctctatct gtagaaatac tcattactaa
      421 atgtatattg gtcttattgg taatattatc taaatacttt ttaatatcat ctagcttttc
      481 cttgtttctt tgactggtgc ctaaagtatc aattaagata gtgtcacaat ctctcagctt
      541 ccttatggta gcttgcatat ccttaggttc ttgtactact tcgcagggta catttagtat
      601 ttctgcataa gtatttaact gttctacagc tccaatccta taggtatcta tggtaattag
      661 gcctacactt cttttctttt ccaaagattc tctagccgct attttagcta tagtggtggt
      721 cttacctact ccagtaggtc ctacaaaaac atttactttt ctagttctta ccttcttttc
      781 aaaaccattg tctttgaagg ccattttcat aaagttttta atttcttctg tagttaagta
      841 ttcatcctct ttcaaatgta gcttacaata gcctaaaagc tggcttttag tagtatcact
      901 tatattttta aagactggat ttttttctat taggttatgg actgagtatc tggtctcatt
      961 gttaaccaca accttttctg ccggctgctt ctttctatta gactcaacag ccactgttac
     1021 ctccagtttc ttttttttga aaggattata ccattttccc acccttatag gtcgttggct
     1081 aataattaca gcctccttac caagttcata actggctcta attaaggctt ccttcatatc
     1141 atttacaata tatgttttta tgatcatacc ttatcaacca tccccactgc ttctatttcc
     1201 acatcattgg gcacatcatt taaggataaa acaggtagac ctggaaagtt aaaggatatt
     1261 aaattcctaa aggccaccct tatatttggt gaagacaata taactccctg gataccctga
     1321 gataggaggg cgttatttaa gtcatttata ttatcaaaga ttctagttat aatatctggt
     1381 ttaagtacag gtatacttcc agacatggtc ttttgaatat tgttggctat taattcctcc
     1441 aggtctggat ggatggttat aactgtaagt acacctttgt catttacata tttattgact
     1501 atggtcctat ttagggcatg tcttacatat tcagttagca tttcaatatc ttttgttttg
     1561 gtaccattgt ctgctaaaac ttccagtatg gttaccaggt cattgattgg tatatcttcc
     1621 tttagtagat tttgtagtac tttttgtact tctcccaaac ttaatatatc tggaattagc
     1681 tcatctacca ccacattgta tctttcctta attccttcca atagttgttg tacctcttgt
     1741 cggcctaata actcatggct atggtttctt ataacttcct tcagatgagt taccaaaaca
     1801 gtggccggat caactattgt atagcctaat agatctattt tctccctatc tttttcatct
     1861 acccaaattg catccaaccc aaaggccggc tctttggtag gtataccttc tatatcaaaa
     1921 tcttccactc ctggatctat taccaaatat ttatttaaat ggatttctcc tcttccaatt
     1981 tcatttcccc taattttaat tacatattca ttggctccca gctgtagatt gtccctaatc
     2041 ctaatagggt ttacaataat ccccatttca ttggcacact gttttctaat agagctaatg
     2101 tggttaatta gattatcgtg atttccttca tccaccaaag gaattatgcc atatcctatt
     2161 tccagggcaa taggttctac ctggaaagtc ataactcctt cttccctttc ctttactggg
     2221 cttacacttg cctctgcctc tgccatttcc agggctagtt ctgccttttc attctccatt
     2281 aacagatagg aaatgctacc tagcaccaaa cc
//
LOCUS       DATGSI010000325         1558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_351918, whole genome shotgun sequence.
ACCESSION   DATGSI010000325 DATGSI010000000
VERSION     DATGSI010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351918"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1558
                     /locus_tag="VK031_05080"
     CDS             <1..>1558
                     /locus_tag="VK031_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53323.1"
                     /translation="LLSRNGLYTYRKNNLVGKSNVGQYSLSQIYHTLFQQVGSYIDKE
                     RTETTDYDTFFKQILRKGGRVPHNTIGGNISLSGKYTEDDVDLITEIINQAVGGSVDM
                     TADQFLAQLRFTEQRGPVAMTMIRAGVPLDTVVALINQPIVDKYISLLNDVGANNLLN
                     EYRAEQILEQIPASDRGEIIRVDRLWDNIKNPKGAKDQRAIFNEFLHWAVLSDQVFQV
                     QQGINYDRSKFNDPALIGVKATKRERALNNNVITNIDKILDTQFLGNISDNLDNVRGI
                     LSTFMAFESGQVREVINNIISKHTQPGQFMPQEEVQSLVFKIKSHLINYLLQTQENPT
                     NGKPPLSTRLPEMLIDSDTSVANRVYQVFKKLKYNFSELGQSLNNFAPTSTIKGAPIN
                     LALHKRGQDVNTENAITNKIRDFRDSVKFGDDLEDAINGETLYKDFIKLSLIQSGIEN
                     SPISYTSSIPIEDYVELIKPYIDQVEKMSHSDLKAFEDEFLFNQWQSDLVSKVEKAKN
                     ATKGIPKYLQN"
BASE COUNT          598 a          226 c          269 g          465 t
ORIGIN      
        1 cttcttagta gaaatgggtt atatacatat agaaagaata atctagttgg taagtcaaat
       61 gttggacaat actccctttc tcaaatttat catactcttt ttcaacaagt tgggtcttat
      121 atagacaaag aaagaaccga gacaactgat tatgatacat tttttaaaca gatactcaga
      181 aaaggaggaa gagtacctca taatactata ggaggtaata tctctctatc tgggaagtac
      241 acagaagatg atgttgactt aatcacagaa ataataaacc aagcagtagg gggttcagta
      301 gatatgactg ctgaccaatt tttagctcaa ttaaggttta cagaacagag agggcctgta
      361 gctatgacta tgataagagc aggagtgcct ttagatactg tagtagcatt aatcaatcaa
      421 cctatagtag ataagtatat ttcattactt aacgatgtag gagcaaataa tcttcttaat
      481 gaatataggg cagaacagat tttagaacag attcctgcct cagatagagg ggaaattatt
      541 agagtagata ggttatggga taacattaag aatcctaaag gtgcaaaaga tcaaagagct
      601 atttttaatg agttcttaca ttgggctgta ttatcagatc aagtatttca ggtccagcaa
      661 ggaataaact atgatagatc taagtttaat gatcctgcac ttattggtgt taaagcaact
      721 aaaagagaaa gagcattaaa taataatgta ataactaata tagataaaat attagatact
      781 caattcttag gaaatatctc agataattta gacaatgtta gaggaatttt aagtaccttt
      841 atggcatttg agtctggcca agtaagagaa gtaataaata atattatttc aaaacatact
      901 caaccaggac aatttatgcc acaagaggaa gtacaatcct tagtatttaa aattaaatca
      961 catttgataa attacttatt acaaacacaa gagaatccta caaatggcaa gccacctcta
     1021 agcaccagat taccagaaat gctcattgat tctgatacct cagttgctaa tagagtatat
     1081 caagtgttta aaaagcttaa atacaacttc tcagagttag gtcagtcatt gaataatttt
     1141 gctcctactt ctactattaa aggagctcct attaatttgg cactgcataa aagaggtcaa
     1201 gatgtaaata ctgaaaatgc tattactaat aagataagag actttagaga cagtgtaaaa
     1261 tttggggatg atttagaaga tgctataaat ggcgagactt tatataaaga ttttattaaa
     1321 ctatctctta ttcaatcagg gattgaaaac tctccaatat catatacaag ctctatacct
     1381 atagaagatt atgtagagct aattaagccc tatatagatc aagtagaaaa aatgtctcac
     1441 tcagatctaa aagcatttga ggatgagttt ttattcaatc aatggcaatc tgatcttgta
     1501 tctaaggttg aaaaagcaaa aaatgcaaca aagggcattc ctaaatatct acaaaatc
//
LOCUS       DATGSI010000326         3187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_254589, whole genome shotgun sequence.
ACCESSION   DATGSI010000326 DATGSI010000000
VERSION     DATGSI010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254589"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VK031_05085"
     CDS             <1..1121
                     /locus_tag="VK031_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53324.1"
                     /translation="KRNTLNVYRIYSEVIMDFVSAKDDLESYLFDELGEFISYLRVKN
                     SNTVKKHVSNLMNIFEYYLLDTGMLLSDLDQIDLEEFFEVAIEDGFIDSKSDLNSYIT
                     SLKKYLRFLSKKDPIYKDSYKEILTISNNRFFYMNRLSSNDSFLMDRNMQHLISLNEK
                     ALNLVDDLEKFILYIGEKNPPVTLKKRFITKDNLLEINNLLNNKHKTKKKIPNQKDFF
                     LIDFFHKLGLDLKIIKVENEKLTLADKSINFLKLSDEEKYNIVFHYAWNHRLTKDLPK
                     EKGEKTIKEARHGMVKFLSKMKAEEKFSIESMPSTSGEVFSIFMDLFWYLGLLDFNLQ
                     NPDHILVSHLGKKVFSYLYNKYENKERTKVIQLKNYKK"
     gene            1138..3132
                     /locus_tag="VK031_05090"
     CDS             1138..3132
                     /locus_tag="VK031_05090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HLS53325.1"
                     /translation="MKKLSLEDFTKYKYLSNINISPDGKRLGYLGHKVDLKNNSYIST
                     LHIMDTIYGKTSTFPLSCGYKDFLWKDDTTILAIGPSSAKDRGQKENTEPLTSFYEIS
                     LNKRKASCLFKLPLQVDSFKILDDDRLLIRGLYCPRGENYNPLMATDVASEGLYKEKD
                     YRVFNEIPFWSDQAGFSNKIRNRLYLYSISKNQLFGISDEFTNVLDYKVNEDQSKLVL
                     ITNSYTDKAYANTELEIYDLDEERILKISPFKDFKYLYANFFKDRILFIGSNMKKYGL
                     QENPHFYITDYEGYNPMQISKDSFDFGITNSIASDSRYKDLAAIRKEGEYLYFVTTEG
                     DSSFVNRIDMEGNMEKLTTKKGSIDDLDVFRGKVFFIGLRSLKLQEIYSLDEKIETQI
                     TYYNDWVIRERSLSIPEKLTFKTENETLIEGWVIKPVDFRLGKTYPAILYINDGPKAA
                     YGEVFFHEMQYLANQGYVVFFCNPRGSDGRGNKFADLRGKYGLIDYQDILDFTDAVLE
                     KYHFIDKRNLGVTGRSYGGFMVNWIIGHTSKFKAAVSQDGICNWISSFLTANDNYYFL
                     HDQIGGTPWEATERLWNHSPLKYADQVTTPTLFIHSKGDSEAYLSESSQMYVALKYHG
                     VESRLYILNDGICKIGNLDKPKCKINRLKIIISWFDKLLK"
BASE COUNT         1143 a          447 c          577 g         1020 t
ORIGIN      
        1 taaaaagaaa caccttaaat gtttatagga tttattctga agttattatg gattttgtca
       61 gtgctaagga tgacctagag tcctatctat ttgatgaact aggggaattt attagttacc
      121 taagggtcaa aaattccaat acagtaaaaa aacatgtatc taatcttatg aatatttttg
      181 aatattacct gctggataca gggatgctcc tatcagactt ggaccagatt gatttggaag
      241 agttctttga agttgccata gaagatggat ttatagattc taagtctgac ctaaactcct
      301 atattaccag cctaaagaaa tatttacgat tcttaagtaa aaaggacccc atatataagg
      361 atagctacaa agagatccta accattagca ataatcgctt tttctatatg aacagactta
      421 gctctaatga ttctttttta atggatagga atatgcaaca tcttatttcc ttaaatgaaa
      481 aagctcttaa cttagtagat gatcttgaaa aatttatttt atatattgga gagaaaaatc
      541 cgcctgtaac cttaaagaaa agatttatta caaaagataa cctactagag ataaataatt
      601 tattaaacaa taaacataaa actaaaaaga aaattccaaa tcaaaaagat ttcttcctta
      661 ttgacttttt tcataagctg ggacttgact tgaaaatcat caaggtggaa aatgaaaaat
      721 taaccttagc tgataagagt ataaacttcc taaagttaag cgatgaagag aaatacaata
      781 tagttttcca ttatgcatgg aatcataggc ttacaaaaga tttgccaaag gaaaaagggg
      841 aaaagactat aaaagaagcc aggcatggta tggtcaaatt tctctccaaa atgaaagcgg
      901 aagaaaaatt ttctattgaa tctatgcctt ctactagcgg agaagttttt tcaatcttta
      961 tggacctttt ctggtatctt ggtcttttag attttaatct tcaaaatcca gaccatatac
     1021 ttgtaagcca tttaggtaag aaggtcttta gctacctata taataaatat gaaaataagg
     1081 aaagaaccaa agtaatccag cttaaaaatt ataaaaaata gatggtggag gggtattatg
     1141 aaaaagctgt ccctagaaga ctttactaaa tataaatatc tatctaatat taatatttca
     1201 ccagacggaa aaaggctggg ttatttagga cacaaggtgg atttaaaaaa taatagttat
     1261 atatctaccc ttcatattat ggatactatt tatggtaaaa ccagtacttt tcccctatcc
     1321 tgtggatata aagattttct ttggaaggac gatactacta tccttgccat aggtcctagc
     1381 tcagctaaag ataggggcca aaaagaaaat acagagcccc ttacttcttt ctatgaaatt
     1441 agtttaaata aaagaaaggc ctcctgttta tttaaacttc cgttgcaggt ggatagcttt
     1501 aaaatccttg atgacgatag gctacttatt agaggtttat attgtcctag aggggaaaat
     1561 tataatcccc taatggctac agatgtagcc agtgaaggtc tttataagga aaaggattat
     1621 agggtcttta acgaaatccc tttttggtca gaccaggcag gtttttcaaa caagataaga
     1681 aatagattat acttatacag tatttctaaa aatcagctgt ttggtataag tgatgaattt
     1741 accaatgtcc ttgactataa agtaaatgag gatcagtcta aacttgtatt aattactaat
     1801 tcctatactg ataaggccta tgccaatact gaattagaaa tatatgattt agatgaggaa
     1861 agaatcctta aaatatctcc ttttaaagat tttaagtatt tatatgctaa tttctttaaa
     1921 gataggattt tgtttattgg atctaatatg aaaaagtatg gcttgcaaga aaatcctcac
     1981 ttttacataa ccgattatga aggctataat cctatgcaga tttccaaaga tagttttgat
     2041 tttggtataa ccaattctat agcttcagat tctaggtata aggacttagc tgccataaga
     2101 aaagagggag aatatcttta ctttgttact actgagggag attcttcttt tgttaatagg
     2161 atagatatgg aaggcaatat ggaaaaacta actaccaaga aaggatctat agatgacttg
     2221 gatgttttta gggggaaagt attttttatt gggctgagaa gcttaaaact acaagaaata
     2281 tactctctag atgaaaagat tgaaacccag attacctatt acaatgattg ggtcattagg
     2341 gaaagaagcc tatctattcc tgaaaaatta acctttaaaa ctgaaaatga aaccctaatt
     2401 gaaggctggg ttataaaacc agttgatttt agattaggta aaacatatcc tgccatttta
     2461 tatattaatg atgggcctaa agctgcctat ggagaagtgt ttttccatga aatgcaatat
     2521 ttagccaatc aagggtatgt agtatttttt tgcaatccta gaggtagcga tggccgtgga
     2581 aataagtttg cagaccttag gggcaaatat gggcttatag attatcagga tattttagac
     2641 tttacagatg cagtattaga gaagtatcat tttatagaca aaagaaatct aggtgttacc
     2701 ggcaggtcat acggtggatt tatggtcaat tggattatag gtcatactag taagtttaaa
     2761 gcagcagtat cccaggatgg aatatgcaac tggatttcta gcttcttaac tgccaatgat
     2821 aattattatt ttctccatga tcaaataggg ggaactcctt gggaagctac tgaaaggtta
     2881 tggaatcact cacctttaaa atatgctgac caggttacaa ctccaacctt gtttattcat
     2941 tcaaagggag attctgaggc ttatttgtct gaatctagtc aaatgtatgt tgccttaaag
     3001 tatcatgggg tagaatcacg cctatatata cttaatgatg ggatatgtaa aattggtaat
     3061 ttagataaac ctaagtgtaa aataaacagg ctaaagataa ttataagttg gtttgataaa
     3121 cttttaaagt aatgtgaaag aggtgtttac aattaatagt ttgctagaga aagctatgag
     3181 gctaaag
//
LOCUS       DATGSI010000327         1342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_446643, whole genome shotgun sequence.
ACCESSION   DATGSI010000327 DATGSI010000000
VERSION     DATGSI010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446643"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VK031_05095"
     CDS             <1..361
                     /locus_tag="VK031_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53326.1"
                     /translation="SMILYEIENESKGDPISSILFGVPKQAWVTKGYPLDSQTLYNRY
                     YFNQIEETTDINLITVEADVFLNEYDINTLDFRNPIYLQSPYGSAYFKLLKVEYTDSN
                     YPSTVLLQKVVLPDTNR"
     gene            578..>1342
                     /locus_tag="VK031_05100"
     CDS             578..>1342
                     /locus_tag="VK031_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53327.1"
                     /translation="MATNNNNREKEVLIKVKTDTSESSKGLDELRSKIKNTSSTAREM
                     DRLDYGKLSVKDLKKELDKAEMSMEQLAKSGNATDGALKVIGNRVDTINNSLKEIKTD
                     KAYEVFRGQVRGATGAIMAMEGAMATLGIESKSFEQTVQTIMGIRAFRRGLDDLIKYR
                     KDMLKLVGVTNEQTVASEAQATATNKATIATRAQTVASKALRVSLSALGIGLLVSALS
                     YLITNWERVRDTIFQLIPGLEDVSKWFSKLTGGGDKL"
BASE COUNT          512 a          174 c          274 g          382 t
ORIGIN      
        1 tagtatgatt ttatacgaaa tagaaaatga atctaaagga gaccctataa gctctatatt
       61 atttggagtt ccaaaacagg cgtgggtaac aaaaggatac ccgttagata gtcaaactct
      121 gtataataga tattacttca atcagataga agagactaca gatataaact taataacagt
      181 agaagctgat gtgttcttaa atgagtacga tataaacaca ttagatttta ggaatcctat
      241 atatctacaa tctccttatg gaagtgctta ctttaaacta ctaaaagtgg aatatacaga
      301 ctctaattat ccgagtacgg tacttctaca aaaggtggtt ttaccagaca caaataggta
      361 aaaagatttg tagtaataat ttgattaaga tattatagta tatagagctt actcatagaa
      421 attactacat ttataggtgt ttaaaagtat agtagtcaat gagttataaa aataaagtga
      481 tttatatcac attttagtaa aagaatagtt gtaggaaaaa attactacaa agtataaaaa
      541 tgggataatc tccattaact aataaagaga tattaaaatg gcaactaata acaataacag
      601 agaaaaagaa gttttaatta aggttaagac agatacttct gaatctagta aaggcttaga
      661 cgaattaagg tctaagatta agaatacttc tagtacagcc agagagatgg ataggttaga
      721 ctatggtaaa ctctctgtaa aagacttaaa gaaagagcta gataaggcag aaatgtctat
      781 ggagcaacta gctaagagtg gtaatgctac tgatggtgct ttaaaagtaa taggtaatag
      841 ggtagatacc ataaacaact ctctaaagga gattaaaacc gataaggctt atgaagtatt
      901 cagagggcag gtaagaggtg ctactggtgc tataatggct atggaaggag ctatggctac
      961 attaggtata gaatctaaat cctttgagca gactgtacaa actataatgg gaatcagagc
     1021 ctttagaagg ggtttagacg atttaattaa gtacaggaag gatatgctta aactagtagg
     1081 cgtaactaat gagcaaaccg tagcttcgga agcacaggct acagctacta acaaagctac
     1141 tatagctact agagctcaaa ccgtagcttc taaggcatta agagtaagtc tatcagcatt
     1201 aggtatcggt ttactggtat cagccttatc ttacctaata actaactggg agagggttag
     1261 agatactata tttcagttaa ttcctgggtt agaggatgtt agtaagtggt ttagtaagct
     1321 tactggaggg ggagataagt ta
//
LOCUS       DATGSI010000328         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_20723, whole genome shotgun sequence.
ACCESSION   DATGSI010000328 DATGSI010000000
VERSION     DATGSI010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20723"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VK031_05105"
     CDS             <1..521
                     /locus_tag="VK031_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS53328.1"
                     /translation="FQNIRLFQNMTVLENVLMGFHNGLKYSVGTALLRLPFYYKHESH
                     THKEAIELLKKMKLLDKKDELAKNLPYGDQRRLEIARALAAKPKLLLLDEPAAGMNPR
                     ETQELTELIQWIRDSFDLTIILIEHDMSLVMNICERVFVFNYGNLVADGTPKEVQSNE
                     EVIKAYLGEDVV"
     gene            521..1225
                     /locus_tag="VK031_05110"
     CDS             521..1225
                     /locus_tag="VK031_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS53329.1"
                     /translation="MLRLTDVNVYYEAIHALKDLNLYINEGEIVTLIGSNGAGKSTTL
                     KAISGLKRPKSGSIKFEDTRIEGLSAPYIVNLGISHVPEGRRIFSRMSVMENLEMGAF
                     NRKDKAGIKKDYDKVFSLFPILSDRKNQMAGTLSGGEQQMLAIGRALMSRPKLILFDE
                     PSMGLAPLIVKEIISIIQDINREGTTVLLVEQNAQTALSIADRAYIIVNGRIEIEGKA
                     QDMLRDDRVKEAYLNG"
     gene            1336..1746
                     /locus_tag="VK031_05115"
     CDS             1336..1746
                     /locus_tag="VK031_05115"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HLS53330.1"
                     /translation="MKKNKFIFIIFCVLSLVLTACTGKDKIEYRDGSFDAEAEPDENG
                     WKGQISIKVEGGKIAKVDYDEIDSAGEKKSKDTDYAKAMESSSGVTPADAFKQLEEAL
                     INSQDPDKIEAVSGATASSASFKKLAKEVLEGIK"
     gene            1939..2457
                     /locus_tag="VK031_05120"
     CDS             1939..2457
                     /locus_tag="VK031_05120"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HLS53331.1"
                     /translation="MDLKSKIRVIEDFPVEGISFKDITTLIKDKDAFKHAVELVAKDL
                     EEKEVDYIAGPEARGFLFGAAVAYSLGIGFIPVRKPGKLPGGTIGYEYDLEYGTNRLE
                     IHKDAIEKGKKVAIVDDLLATGGTMLATAKLIEELGGKVVAMEFLIELESLKGREKLK
                     NYYVNSLVKYDK"
     gene            2482..>2896
                     /locus_tag="VK031_05125"
     CDS             2482..>2896
                     /locus_tag="VK031_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HLS53332.1"
                     /translation="MSADIDKREKLNILHIDMDAFYAAVEEQDNPSLIGRPVIVGGTK
                     RHGIVTTANYEARKYGIHSAMPIYLAKAKCPHGYYLPTRMERYREVSNEVFETLYEIT
                     DQIEPVSVDEAYLDIENLGQDPVRVAKEIKRRVLKK"
BASE COUNT         1036 a          402 c          658 g          800 t
ORIGIN      
        1 ccttccaaaa tattaggtta ttccagaata tgaccgtatt agaaaatgta ttaatgggtt
       61 ttcataatgg gttaaaatat agtgtaggaa ctgccttact gagattacca ttttattaca
      121 agcatgaaag ccatacacat aaagaggcaa ttgaactttt aaagaaaatg aagttactgg
      181 ataaaaaaga tgaattggct aaaaaccttc cctatggaga ccaaaggcgg ctggaaattg
      241 ccagagcctt ggcagctaaa cctaaattac ttttactgga tgagccagct gctggtatga
      301 atcctagaga aacccaggag ctaactgaat taattcaatg gattagagat agctttgacc
      361 ttacaattat tctgatagag catgatatga gtctggttat gaatatttgc gaaagagttt
      421 ttgtttttaa ttatggcaat ttagtggctg atgggacgcc taaagaagtt caaagcaatg
      481 aggaagtaat caaggcctat ctgggggagg atgttgtgta atgttaagac ttacagatgt
      541 aaatgtttat tatgaagcaa tacatgcatt aaaagaccta aatctatata taaatgaggg
      601 agaaattgta accctaatag gatccaacgg ggcaggaaaa agcacaaccc taaaggccat
      661 ttcaggattg aaacggccta aaagtggcag cataaaattt gaagatacta gaatagaagg
      721 ccttagtgca ccttatattg taaatctagg tatctcccat gtgcccgaag gtagaaggat
      781 tttttctaga atgtcggtta tggaaaacct agaaatggga gctttcaata ggaaggataa
      841 agccggcata aagaaggatt atgataaggt attttccctc tttcctattt taagtgacag
      901 aaaaaatcaa atggccggta ccttatcagg tggagagcaa caaatgctgg ccataggcag
      961 ggccttaatg tccagaccca aactaatctt atttgatgaa ccttctatgg gattggcacc
     1021 tttaattgta aaagaaatta tatctataat ccaagatata aacagggaag gtactacagt
     1081 tttattggta gaacaaaatg cccaaactgc tttaagcatt gcagataggg cctatattat
     1141 tgtaaatggt agaattgaaa tagaaggcaa ggctcaagat atgttaaggg atgatagggt
     1201 gaaggaggcc tatttaaatg gttaaaaaag cacataggtg ctttttctat tttacaagac
     1261 tatttatggc cacttaagta tatttagact tattttgtct ataatctata ctgtataaaa
     1321 actgggaggt agggtatgaa aaagaataaa tttatcttta ttatcttctg tgtgctatct
     1381 ttagttctta cggcttgtac aggtaaggat aagatagaat acagggatgg cagttttgat
     1441 gctgaagcag aacctgatga gaatggttgg aagggacaga ttagtattaa ggtggaaggt
     1501 ggaaaaattg caaaggtaga ttatgatgaa atcgattcag ctggagagaa gaaatcaaag
     1561 gatacggatt atgctaaggc catggaaagt tcgtcaggag tgacccctgc cgatgccttt
     1621 aaacaattgg aggaggcctt aatcaatagc caggatcctg ataaaataga ggcagtatca
     1681 ggagcaacag ccagcagtgc aagctttaaa aaactggcta aggaagtttt ggaaggtata
     1741 aaatagaaag cctataccct attgtttcaa aacaataggg tataattttt ctataaaagt
     1801 agtagccttt atgatataat tttatatgaa tattagtttg tgaagtcagt gataaaacat
     1861 gaattataga tgaaaaggta tattaaatat gataaactta tatttggcaa aatagagaaa
     1921 atcatggagg gaacaaattt ggatcttaag tcaaaaatta gagtaataga ggattttcca
     1981 gtggaaggaa taagttttaa ggatataacc accttaataa aagataaaga tgcctttaag
     2041 catgccgtag aattagtagc caaggatctg gaagaaaaag aagttgatta tatagcagga
     2101 ccggaggcca gaggcttctt atttggggct gcagtggcct attctttggg aatagggttt
     2161 atacctgtaa gaaaaccggg gaaattacca ggaggcacca taggttacga atatgattta
     2221 gagtatggaa ccaatagatt agagatacat aaagatgcca ttgagaaggg gaaaaaggta
     2281 gccatagtgg atgacctttt ggccacaggt ggaaccatgt tggctacagc taagcttata
     2341 gaggagctag gtggaaaggt tgtggccatg gagtttttga ttgagttaga gtctttaaaa
     2401 ggaagagaaa agcttaaaaa ttactatgtc aactccttag taaagtatga taagtaacta
     2461 cttaggatgt gatcaattat tatgtcagca gacatagata aaagggaaaa attaaacatc
     2521 ctccatatag atatggatgc cttttatgct gctgtggagg aacaggacaa cccatcccta
     2581 ataggaagac cagttattgt aggtggaact aaaagacatg gcattgtgac tacagccaat
     2641 tatgaggcta gaaaatatgg gatacattca gccatgccca tatacttggc aaaggctaag
     2701 tgtccccatg gctattatct gccaactagg atggaaagat acagggaagt ttccaatgaa
     2761 gtatttgaaa ccctgtatga aattacagat caaatagaac ctgtttcagt tgatgaggcc
     2821 tatttagata tagaaaattt aggtcaagat cctgtaagag tagccaagga aattaaaaga
     2881 agggtgctta aaaaaa
//
LOCUS       DATGSI010000329         1847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_446743, whole genome shotgun sequence.
ACCESSION   DATGSI010000329 DATGSI010000000
VERSION     DATGSI010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446743"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /gene="amrB"
                     /locus_tag="VK031_05130"
     CDS             complement(<1..1176)
                     /gene="amrB"
                     /locus_tag="VK031_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HLS53333.1"
                     /translation="MGKILAAYLFPHPPIIIPEIGRGEERRVKKTIDGVRALSQDISV
                     KKPDTIIVITPHGPIFSDAIAISVEEKLYGDFGRFGFSRINFNFENNLNLVKEIIKRS
                     NREGIIMAELDMNFARDYNVDHEIDHGVLVPLYFVDKLYRNYKLIHITYGMLPPKDLY
                     RFGKIIQGVVKESQEQVVVLASGDLSHRLSNDGPYSYSPEGKKFDEAIVDILKRGDLE
                     DIVAFDFNLAEKAGECGLRSLMVMAGTLDRLKFDSTVLSYEGPFGVGYATAKLEVTGE
                     KKGEDLVGKIEQRDRERIKRIRQKESEYVRLARKSLEYFLKHGKYMPVPENLSPKLLR
                     EKKAVFVTLKKDGLLRGCIGTTEPREDNVALEIIRNAVSAGTEDPRFPKVEQDELDKL
                     "
     gene            complement(1249..>1847)
                     /locus_tag="VK031_05135"
     CDS             complement(1249..>1847)
                     /locus_tag="VK031_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HLS53334.1"
                     /translation="KLRVENMIPGVIYKRGEETRHIQVKDSDFRRVYRVAGTTSIIDL
                     ELDGKTHPVIIKELQSHPVKNEYLHIDFQELDMDETLRVSVPINLINRENIKVQPSVL
                     TQLLDEVEIECLPGDIPSSIDVDVSEMDFTTPIFVQDLDVAKDDKITVWTELDTVVCT
                     LTEPSMEEEEELEEDPLLEDAADVPLVSDEEEEEVEEE"
BASE COUNT          484 a          425 c          264 g          674 t
ORIGIN      
        1 taacttatcc agttcatcct gttccacctt aggaaaacgt ggatcctctg tgccggcact
       61 tactgcattt cgaattatct ctaaggccac attgtcttcc cttggttctg tagtgcctat
      121 gcagcctctt aagagaccat ccttttttaa ggtcacaaat actgcttttt tctccctcaa
      181 taatttaggt gaaagatttt cgggtactgg catatatttg ccatgcttta agaaatactc
      241 tagacttttt ctagccaatc ttacatattc actttctttt tgcctaattc ttttaatcct
      301 ttctctatcc ctttgctcaa tttttcccac cagatcctcc ccttttttct cacctgtaac
      361 ctctagttta gcggtggcat agccaacacc aaaaggtcct tcataagaaa ggacggtgga
      421 atcaaattta agcctatcca aggttcctgc cataaccatt aaagacctta acccacattc
      481 tcctgccttt tcagctagat taaaatcgaa ggctactata tcctccaggt ctcccctttt
      541 taaaatatct actatggcct catcaaattt tttgccctcc ggtgaataac tatagggccc
      601 atcattggat aaacgatggg atagatctcc actggctagg actaccacct gctcttgaga
      661 ctccttaact accccttgga ttatcttccc aaacctgtat aggtcctttg gtggcaacat
      721 gccgtaagtt atgtggataa gtttatagtt tctatatagt ttatccacaa agtataaagg
      781 taccaacacc ccatggtcta tttcatgatc aacattataa tctctggcaa agttcatgtc
      841 caattctgcc attataattc cttccctatt agatcttttt attatttctt tcaccaggtt
      901 taagttgttt tcaaagttaa aattgatcct actaaaaccg aatctaccaa aatctccata
      961 tagcttttcc tcaacagaaa tagctatggc atcggaaaaa atcggtccat gtggggtaat
     1021 tactattata gtgtcaggtt tcttaacact tatatcctga cttagtgccc taactccatc
     1081 aatagtcttt tttacccttc tttcttctcc ccttccaatc tcaggtataa taattggagg
     1141 atggggaaat aaataggctg ccagtatttt acccatctaa tccctcccaa tcttcattat
     1201 tttagataca aaaaaacaga taaggtggcc ttatctgttt tttacatctt attcttcttc
     1261 tacttcttcc tcttcttcat cggaaactag tggtacatct gctgcatcct ccagtagtgg
     1321 atcctcttct aactcttctt cttcttccat agaaggttct gtcagagtac atactactgt
     1381 atctagttcg gtccaaacag ttattttatc atcttttgct acatctaggt cttgtacaaa
     1441 tattggtgtt gtaaagtcca tttcagatac atccacatct atactacttg gtatatctcc
     1501 tggtagacac tctatttcta cttcgtccag tagctgtgtt agtactgatg gttgcacctt
     1561 tatgttttct ctgttaatta agttaattgg aactgatact cttaatgttt catccatgtc
     1621 tagctcttga aaatctatgt gaagatattc attttttact ggatgacttt gtagttcctt
     1681 tataataact ggatgtgtct taccatctaa ttctaagtca attatggatg tagtacctgc
     1741 aactctataa actcttctaa aatcggaatc tttaacctga atgtgtctag tttcttctcc
     1801 tcttttatag attacaccag gaatcatatt ttctacacga agtttat
//
LOCUS       DATGSI010000330         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_543943, whole genome shotgun sequence.
ACCESSION   DATGSI010000330 DATGSI010000000
VERSION     DATGSI010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543943"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VK031_05140"
     CDS             <1..664
                     /locus_tag="VK031_05140"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HLS53335.1"
                     /translation="PIIPSGTEIIGISIDEDTGICKVDFSAEIKNIETLKEEENLIKG
                     IVYTLTEFPAIEEVQLMVEGEIVPSLKHDIAINNPLGRENINYIGSREEGISNVVVYY
                     KGEANDEFDYFVPVTIPTMAPRPNVNTALELLLEGPPADTGLRTDLPENISLAAIDIR
                     EGTAFIDLDLEGDKLSQGEINSIIKNIGLTLSEFEEIDKVEILIDGQVVNTSVPAFAN
                     EY"
     gene            838..1572
                     /gene="rph"
                     /locus_tag="VK031_05145"
     CDS             838..1572
                     /gene="rph"
                     /locus_tag="VK031_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HLS53336.1"
                     /translation="MRANGRRFDQLREVKLTRNYLMHPYGSVLIEMGNTKVICTAMVD
                     DRVPPFLKGTNSGWITSEYSMLPGSTNHRKIRDSSRGRIDGRTQEIQRLIGRSLRTVV
                     DLSKIGERTIWIDCDVIQADGGTRTAAITGGFVALADALYRLHKEEKIDTVPISNYLA
                     AISVGIGKEGPILDLCYQEDHKALVDMNIVMTDDGKFIEIQGTGEEGSFSREELNALL
                     DLGEKGNKELIDMQKQALGPIGDLIG"
     gene            1588..2199
                     /locus_tag="VK031_05150"
     CDS             1588..2199
                     /locus_tag="VK031_05150"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HLS53337.1"
                     /translation="MGQKYLVLSTNNMHKLKEIRDILSDLPVKVLSKKALNLEEFEIK
                     EDGNSLEENSIKKAQALAKLTDHMVMADDSGLFVEALDGKPGIHSSRYAGEEGNDEKN
                     NQELLNALEGLPMEKRKAEFKTVIALVTDKRELITIEGSCSGHIAFKPKGDNDFGYDP
                     LFIPTGYDKTFAQLDPKIKNKISHRADAIRKLKEILEDLLKDD"
     gene            2202..>2539
                     /locus_tag="VK031_05155"
     CDS             2202..>2539
                     /locus_tag="VK031_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HLS53338.1"
                     /translation="MRILAVSDTHGRCEGIIKEVKNREKFDLLFHLGDYVEDGDKIAQ
                     ELDIPAVIVRGNGDFYSHYQDEELVEFSGKTIFLTHGHMYNVRFDISNLYYRGRELGA
                     DLILFGHTHVP"
BASE COUNT          941 a          372 c          571 g          655 t
ORIGIN      
        1 acctattatt ccaagtggca ctgaaattat tgggatctcc atagatgagg atacaggaat
       61 ctgcaaggtt gatttctctg cggaaataaa aaatatagaa acattaaaag aagaagaaaa
      121 cctaataaag ggtattgtct atacactaac agagtttcct gccatagaag aggttcagct
      181 tatggtggag ggagaaatag ttccaagctt aaagcatgat attgctatta ataatccctt
      241 aggtagagaa aacataaatt atataggtag tagagaggaa ggcatatcca atgtggttgt
      301 ttactataaa ggcgaggcca atgatgaatt tgattatttt gtaccggtaa ccataccaac
      361 tatggcacca agacctaatg taaatacagc cttggagctc ttgctggaag ggccaccagc
      421 tgatacaggt ttaagaacag atctacctga aaatattagc ctagctgcca tagatataag
      481 agagggcacc gcatttatag atttggacct ggaaggtgat aagttaagtc aaggagaaat
      541 aaactccata ataaaaaata tagggctgac tttaagtgag tttgaggaaa tagataaggt
      601 ggaaatatta atagacggcc aggtggtaaa tacttctgta ccagcttttg caaatgaata
      661 ctagaataat taaatccaat ctgctaaaat aacaatttag gccataaaaa ttaagggaga
      721 gctttgcttt ccttttttca ttgtcttttc ttgctaagcc aggttctagc ccttgtgata
      781 gataaaaaat aaaaaatttg ataaaataaa actaatagat aaataggaga tgtgaaaatg
      841 agagcaaatg gaagaagatt tgatcaatta agagaggtaa aattaactag aaactattta
      901 atgcatccat atggatctgt acttattgaa atgggaaata ctaaggtgat ttgtactgct
      961 atggtagatg acagggttcc gcccttttta aaaggtacta attctggctg gataaccagt
     1021 gaatattcta tgttgccagg ttcaacaaac cataggaaga taagagactc ttccagaggg
     1081 cgaatagatg gtaggacaca ggaaatccaa aggcttatag gcagatcctt aagaactgta
     1141 gtagacttat ctaaaatagg agaaagaacc atttggatag attgtgatgt aatccaggct
     1201 gatggaggca caagaactgc tgccattaca ggaggctttg tagccttggc tgacgctttg
     1261 tataggttac ataaggagga aaaaatagac acagtaccta tctctaacta cctggcagca
     1321 atcagcgtag gcattggaaa agaagggccc atactggatt tgtgttacca ggaagaccat
     1381 aaggccctgg tagacatgaa tatagtaatg actgacgatg gaaaatttat agaaatccaa
     1441 ggaactggtg aagaaggaag cttttccaga gaagaactaa atgccctgtt agatttagga
     1501 gaaaaaggga ataaagaatt aatagacatg caaaaacaag ccctaggtcc tataggggat
     1561 ctaatagggt agaaggagaa tattttcatg ggacagaagt atttggtact ttcaacaaac
     1621 aatatgcata aacttaaaga aataagagat attttaagtg atctgccagt aaaggtatta
     1681 tctaagaaag ctttaaactt ggaagaattt gaaataaaag aggacggaaa tagtttagag
     1741 gaaaatagta taaaaaaggc ccaagccttg gccaaattaa ctgatcatat ggtaatggca
     1801 gatgattcgg gcctatttgt ggaggcttta gatggtaaac ctggcataca ctcatccagg
     1861 tatgctggag aggaaggaaa tgatgagaaa aacaatcaag agcttttaaa tgccctggaa
     1921 ggtttaccta tggaaaaaag aaaggcagaa tttaaaacag taatagccct ggtaacagat
     1981 aagagggaat taataaccat agagggaagt tgttcaggcc atatagcctt caaacctaag
     2041 ggagataatg attttggtta tgatccctta tttataccaa ctggctatga caaaaccttt
     2101 gcccaactag acccaaagat aaaaaataaa ataagccaca gggctgatgc aataagaaaa
     2161 ctaaaagaaa ttttagaaga cttgctgaag gatgattaag gatgagaata ttagcagtaa
     2221 gcgatacaca tggcaggtgt gaaggcataa taaaagaagt taaaaataga gaaaaatttg
     2281 atctactctt ccatttagga gattatgtag aggatgggga taagattgcc caggagttag
     2341 atatcccagc tgtcatagta agagggaatg gagactttta tagccattac caggacgagg
     2401 aactggtaga atttagcggc aaaaccatat ttctaaccca tggacacatg tacaatgtaa
     2461 ggtttgatat atctaatttg tattacaggg gcagggagct gggagccgat ttgatcttat
     2521 ttggccacac ccatgtacc
//
LOCUS       DATGSI010000331         3404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_638394, whole genome shotgun sequence.
ACCESSION   DATGSI010000331 DATGSI010000000
VERSION     DATGSI010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638394"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VK031_05160"
     CDS             complement(<1..980)
                     /locus_tag="VK031_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53339.1"
                     /translation="MKRLGVLIVCLALILTSLPIAPIVAFGEESTNDNIKIDTSGYTV
                     IDGIEDYEVDGSKITLKLKDGNKTQISFLSHNIFRYYVDINDSEFLEYPNPRSRNHEA
                     TIVARPDSDWFDEVGVRPVVEDGDVLKISTDKVTLLIDKKTSQLTVKNKSGEIVLEEV
                     APIMYKPGQTIQSLSEGSDEYFFGGGMQNGRFTHKGESIKIENTNNWVDGGVASPNPF
                     YWSTKGYGVVRNTFQPGEYDFGNTNDGIVQTRHNENRFDAYIFVADDPVGILNEYTNL
                     TGKPAHLPDYAFYLSHLNCYNRDAWKEVPKGTSGAKHIDGKYYVETNRGG"
     gene            complement(1574..2506)
                     /gene="pfkB"
                     /locus_tag="VK031_05165"
     CDS             complement(1574..2506)
                     /gene="pfkB"
                     /locus_tag="VK031_05165"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HLS53340.1"
                     /translation="MILTVTLNPSIDRRYNVQNFEKGNIFRTNKYQYTAGGKGLNVAK
                     VIENLNERVIATGLIGGRNGEFIKEELDKRGIKYQFTPINGETRSCLAILSNDGSQTE
                     ILEGGPLISKAEMEEFLQLYDLLIEDVDIICASGSIPQGVSTDIYKDLIKRAKRKGKK
                     LILDTSGAPLKAAIEMAPSIIKPNLEELEALVGNKMTEKQDIVESGKELLERGIEIVV
                     ISLGKEGVMALNDGHLYQVTVPEIRAVNPVGSGDSLVGGLAVAIGRGYDLEQSLRLAA
                     ACGTANAMEAETGKVDLKNVEALLNKIQLKKTKI"
     gene            complement(2546..3400)
                     /gene="fba"
                     /locus_tag="VK031_05170"
     CDS             complement(2546..3400)
                     /gene="fba"
                     /locus_tag="VK031_05170"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HLS53341.1"
                     /translation="MNIVPSKDMLWDAKKNGYAIPAFNIHNLETLQAVLEGAWEMRSP
                     VMIATTPGTVNYAGVDFLVAMVKAGARMYDIPIALHLDHCTDIDFINECINAGYRSVM
                     IDASMKDFEDNIEISKSVVELAKKHNVTVEAELGRVGGEEDGIKVEKKDEALTDPDKA
                     LEFVDRTGIDSLAVAIGTAHGMYRFEPELDFERLIRINKLVDIPLVLHGGSGVPNESI
                     TTAIRHGICKVNIATELKIPFAESIREYFATNPEADDPRKYLTPGKERVKQVVIEKIK
                     LCGSGNRA"
BASE COUNT          943 a          778 c          463 g         1220 t
ORIGIN      
        1 cttccacctc tattagtttc aacataatac tttccatcta tatgcttagc accacttgta
       61 ccctttggta cctccttcca agcatctctg ttgtaacaat tcaaatgtga caaatagaaa
      121 gcatagtctg gtaggtgtgc aggttttcca gttaggttag tatattcatt taaaatacct
      181 actgggtcat ctgcaacaaa aatatatgca tcaaatctat tttcattatg tctagtttga
      241 actataccat cattggtatt gccaaaatca tattcacctg gttggaaagt gtttcttaca
      301 accccgtatc ctttggtaga ccaatagaat ggattagggc ttgccactcc cccgtcaacc
      361 cagttgttag tgttttctat ttttatactt tcgcctttat gtgtaaaacg accgttttgc
      421 atcccaccac cgaaaaaata ttcatcagaa ccttctgata aactttgaat ggtttggcct
      481 ggtttataca taataggcgc tacttcttct agtacaattt cccccgattt gttctttacg
      541 gttaattggg acgtcttttt atcaataagc aatgtaactt tatctgtcga aatttttaag
      601 acatcaccat cttcaactac tggcctaact cctacttcgt cgaaccaatc actgtctggt
      661 cttgccacga tggttgcctc atgattccta cttctgggat ttggatactc tagaaactcg
      721 gaatcgttaa tatcaacata atatctaaat atgttatgtg ataaaaatga tatttgagtt
      781 ttatttccat ctttcagttt taaagtaatt ttactcccat caacctcgta gtcctcaatc
      841 ccatcaataa cggtgtatcc actcgtatca attttaatat tgtcatttgt gctctcctca
      901 ccgaatgcaa caattggtgc gataggtaaa gatgtcaata ttaatgctaa acaaactatc
      961 aatacaccta gtcttttcaa accttttacc ccccttgaaa ttagatttag cgttcacaac
     1021 tcatttaacc ggcaaccaaa taataatgtt cactatttgg tcttaagtgt attctaatat
     1081 caatatccta cacatatatc cccccttttg taattatcac actataaaga acctcaataa
     1141 attaaagcag ccatgatgta atgttgtcat taccaggctg ctacttaact tacctttatt
     1201 tatttttaac tacttgccag tcgatttctg atattaaaat ggtatcatag ggtagctgtt
     1261 taaatgcagt aaaatccttg tatgtactta aaaatatctg acgtgatata tatatatata
     1321 tgatcattcg acagtgcttt actttgctta gtgaagggca ggtggtaatt accacctgct
     1381 tttatactat tatatttata agtgcttgtc aagcctaaaa atcatttaat ggcaatcttt
     1441 tatagaatga tcaatcattt gtgacatata cttttgaaaa atttctccta tttagattta
     1501 gcagattgat catagcattc ttttggacat aaaccttaga caattttggg taatcttaaa
     1561 atatccgctc ttgctaaatt tttgtttttt tcagttgtat cttgtttagc agtgcttcta
     1621 cgttttttaa atctacttta ccagtctcag cctccatagc atttgcagtt ccgcaggcag
     1681 ctgccaatcg caaactctgc tccaaatcat agcccctccc tatggcaaca gctaacccac
     1741 ctaccagcga atctccagaa cctactgggt taacagccct tatttcaggc acagtaactt
     1801 ggtataagtg cccgtcattc aatgccataa ctccttcctt tcctagagat attaccacaa
     1861 tttctatacc tctctctaac aactccttgc ctgactcaac tatatcttgt ttttctgtca
     1921 tcttatttcc aactagggcc tccagttctt ctagattagg cttaattata gaaggagcca
     1981 tctctatagc agcttttagg ggagcaccac tcgtatctaa tattagtttt ttccctttcc
     2041 ttttagctct ttttattaga tctttatata tatctgttga tactccctgg ggaatgcttc
     2101 ccgatgcaca tattatatct acatcttcta tcaataggtc ataaagctgt aaaaattctt
     2161 ccatttcagc cttactaatc aacggaccac cttccaatat ttcagtttga ctaccgtcat
     2221 tagacaaaat tgccaaacaa cttcttgttt ctccatttat aggtgtgaat tgatatttga
     2281 ttcccctctt atccagctct tccttgataa actccccatt tcttccacct atcaatccag
     2341 ttgctataac tctttcatta aggttttcta tcactttagc tacatttaag ccttttcccc
     2401 cagccgtata ctgatattta ttggttctaa atatgtttcc tttttcaaaa ttttgaacat
     2461 tgtaccttct atctatagaa ggatttaagg ttacagttag tatcatatat cctcctttat
     2521 atattcagta tgatattgaa aatccttacg ccctattgcc actgccacat agcttaattt
     2581 tttcaataac cacctgttta accctttctt tgccaggtgt taagtacttc ctaggatcat
     2641 ctgcctcagg gttggtggca aaatattccc ttatactttc tgcaaaagga atcttcaact
     2701 cagtagcaat gttcacctta catataccgt gcctaatagc agtagttatg ctttcatttg
     2761 gtacacctga accaccatgt aataccaagg ggatatctac cagtttgttt attcttataa
     2821 gtctttcaaa atctagctca ggttcaaacc tgtacatccc atgtgccgtc cctatggcta
     2881 ccgctaagga gtcaattcct gttctatcca cgaactccaa agccttatca ggatctgtaa
     2941 gggcctcatc ttttttttct accttaatac catcttcttc tccacctact cgccccagct
     3001 ctgcctcaac tgtcacatta tgttttttag ccaattccac cacactttta cttatttcta
     3061 tattatcttc aaaatctttc atggaagcat ctatcattac agatctatat cctgcattta
     3121 tacattcatt aataaaatct atatccgtac aatgatctaa atggagggct ataggaatat
     3181 catacatcct tgcccctgct ttaaccatag ctactaaaaa atccacacca gcatagttta
     3241 ctgttcctgg tgttgtagca atcataactg gcgaacgcat ctcccaggcc ccctccagta
     3301 cagcctggag tgtttctaaa ttgtgtatat taaatgctgg tatagcatat ccattctttt
     3361 tggcatccca aagcatatct ttacttggta ctatattcat ttac
//
LOCUS       DATGSI010000332         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_213585, whole genome shotgun sequence.
ACCESSION   DATGSI010000332 DATGSI010000000
VERSION     DATGSI010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213585"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VK031_05175"
     CDS             <1..710
                     /locus_tag="VK031_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLS53342.1"
                     /translation="YIFRNNTVIHNEALASVDIAVNDLGKSNIGIISIKTDWGVSTAE
                     IVKEIIEKDFPEAKVVAHEEVIEGSDDYTPAISKLDDAGADVVIGVGMYNLVAPVARQ
                     YKEINPDIEIVGFSNAYSQQLLELGGDAVEGVAFPVIFFSQSEDPLVSSFVEEYKERY
                     GSEPSALTAQAYDSVGIFLTAVQNVGSDDTGKIRDEIAKIGYEGVTGYTKFDENGDVE
                     KSFTKVVVKDGQFELLK"
     gene            723..1598
                     /locus_tag="VK031_05180"
     CDS             723..1598
                     /locus_tag="VK031_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLS53343.1"
                     /translation="MSIQQIFNGVTIGMSYALIAVGYSLVFGILRLINFSHGSVYAFG
                     ANIILYFISLNYGIVPALIAGTLLTGLLNIVIDKFAMEPLRKQGAEPIASLITTIGIS
                     NIITNILIVRLGSQKRQFPSLFPSKTLNIFSLDIQYTQLGMFLISLVLMAILVIIINK
                     TKIGLAMRASEQNLKAAKLMGINVNFVISFTFFLGGLCAAIAGALVSGYYGMAYPNMG
                     YMSGLKAFAAAVLGGIGSLPGSIIGGIIVGISESAAASFLGSEFRDSMAFIILVIVLI
                     IRPNGLLGKKEIEKV"
     gene            1600..>2283
                     /locus_tag="VK031_05185"
     CDS             1600..>2283
                     /locus_tag="VK031_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLS53344.1"
                     /translation="MGKLKSLKTKFEKTKPILIGISVLILFIIPFINLNSYYINVINK
                     VLIYVLMALGLNILVGYTGLVSLGQAGFVAIGAYVTTILTTKLELNFFLATFIALLVA
                     AGFGILLGLPTLRLSGPYLTIVTLGFGEIIRTIIIVWDSVTNGPLGIRNIPMPSIFGF
                     ELTVRNGGLYLLTLICTLLVMIFSVKLYKGKVGRAMRAIKVDERASIMMGINTTYYKV
                     LAFVLAAVIC"
BASE COUNT          766 a          370 c          461 g          686 t
ORIGIN      
        1 aatatatatt tagaaataat actgtgattc ataatgaagc attagctagt gtagatattg
       61 cagtaaacga tctaggaaaa tcaaatatag gtataatttc tatcaaaact gactggggtg
      121 taagtacggc agaaatagtt aaagaaatca tagaaaagga cttcccagaa gcaaaagtag
      181 ttgcccatga agaagttata gaaggttcag acgattacac accagccata tccaaattag
      241 atgatgctgg agcagacgta gttataggtg ttggaatgta taacctagtt gcacctgttg
      301 caagacaata caaagaaatt aatccagaca tagaaatagt aggtttctcc aacgcatata
      361 gccaacagct actagagtta ggtggagatg cagtagaagg agtagctttc ccagtaatct
      421 tcttctccca atctgaagat ccactggtat cttccttcgt agaagaatac aaggaaagat
      481 atggtagcga gccaagtgct ctaacagcac aagcctatga ctctgtggga attttcctaa
      541 cagcagttca aaatgttggc agcgatgata ctggcaagat aagagatgaa attgctaaga
      601 taggatatga aggtgtaaca ggatatacta agtttgacga aaatggagac gtagaaaaat
      661 cctttacaaa agttgtagtt aaagacggtc aattcgaatt attgaaataa taggaggcag
      721 aaatgagtat acaacagatt tttaatggag ttaccatagg tatgtcttat gccctaatag
      781 cagttggata ctcactggta tttggcatat taagactaat aaacttctca catggatctg
      841 tttatgcttt tggcgccaat attattttat attttatctc cttaaactat ggaattgtgc
      901 ctgctctaat agcaggcacc ttgcttacag gattattaaa catagtaata gataagtttg
      961 cgatggagcc gctccgaaaa caaggggcgg aacctatcgc atccttaatc accacaatag
     1021 ggatatcaaa tataataact aatattttaa ttgtaaggct aggcagccaa aagagacagt
     1081 tcccatcatt atttccaagt aaaacattaa atatattttc attggatatt caatataccc
     1141 agctaggaat gtttttaatt tctctagttt tgatggcaat attggtaata ataataaaca
     1201 aaacaaagat tggactggcc atgagggcct cagagcagaa tttaaaagct gctaaactaa
     1261 tgggcataaa tgtaaacttt gtaatatcat ttacattctt cctaggtgga ctttgtgcag
     1321 ccattgcagg tgccttagtc agtggctatt acggtatggc ctatccaaat atgggctata
     1381 tgtctgggct taaggccttt gcagcagcag ttcttggagg aataggtagt ctaccaggtt
     1441 ccattatagg tggaataatt gtaggtatat ctgaatcagc agcagcctca tttttaggtt
     1501 cagaattcag agacagtatg gcatttataa tacttgtaat tgtcctaata attaggccca
     1561 atggattatt aggcaaaaaa gagattgaga aggtgtagta tgggaaaatt aaaatcactt
     1621 aaaacaaaat ttgaaaaaac aaaaccaata ctaattggaa tatcagtact aattttattt
     1681 ataataccct ttataaacct aaatagttac tatataaacg taatcaacaa ggtcctaatt
     1741 tatgtgctta tggccttagg gttaaatatt ttagtaggat atacaggatt ggtatcctta
     1801 ggtcaggctg gctttgtagc cataggagct tacgtaacaa ctattctaac tactaagcta
     1861 gaattaaact tctttttagc aacctttata gccctattgg tggcagctgg ctttggaatt
     1921 cttttaggcc tgccaaccct aagattaagt ggtccttatt taaccatagt aaccctaggt
     1981 tttggagaaa ttatcagaac catcattata gtttgggact ctgtaaccaa tgggccctta
     2041 ggaattagaa atattcctat gcctagtatc tttggctttg agctaactgt acgcaacggc
     2101 ggactttacc tattaacctt gatttgcacc cttttagtca tgatatttag tgtaaaatta
     2161 tacaagggca aggtaggtag ggccatgagg gccattaagg tggacgaaag agccagtatt
     2221 atgatgggta ttaatacaac ctactataag gttttggcct ttgtattggc agcagtaatc
     2281 tgt
//
LOCUS       DATGSI010000333         1295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_69565, whole genome shotgun sequence.
ACCESSION   DATGSI010000333 DATGSI010000000
VERSION     DATGSI010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69565"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1295)
                     /locus_tag="VK031_05190"
     CDS             complement(<1..>1295)
                     /locus_tag="VK031_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53345.1"
                     /translation="GLEIISKVMEVLGDSTEEFKDISKALKEIMELIKDFDGPNFETP
                     GDTFLQAKDGLFESIEDVSNSLGQFLENMDKQGRLLMDSMEEMSNQLLRVVNLMFDIL
                     DEIFNREVQLDRIVEDVSTTRIDQVKEGKVYNTRNYGTIEADRNVGGIAGAMSIDLEL
                     DPEDDIDISGRFTANTIFESRAVIDKAENYGDIVGKKNHVGGIVGNMDLGFIRDVISS
                     AKVESTAGNYVGGIAGKSMGPIYSSYSRSSLKGGNYIGGISGLAKEIQKSYSLVDIEE
                     GKAYLGAIAGRLDETNRLKDNYFASDKLSGIDGISYENKAEPIAYDKLINIEGIPSVF
                     KKFKLSFWIEDELVDSYEFNYGDDLTDIDLPSIPEKEGYYGQWEDLDFSNLKFDQDIQ
                     AEYKPFISILESEELRDETLAKVLVEGQFVEGDSLRLKE"
BASE COUNT          367 a          324 c          153 g          451 t
ORIGIN      
        1 ctcttttaat cttaaactat ctccttctac aaattgaccc tccactaaaa ctttagctaa
       61 agtttcatct cttaattctt cactttccag gatgcttatg aaaggcttat attcagcctg
      121 aatatcttga tcaaacttta agttggaaaa atccaaatcc tcccactggc cataataacc
      181 ttccttttca ggaattgagg gaagatctat gtctgttaaa tcatctccat aattaaattc
      241 ataactatca accagttcat cttctatcca gaaagataac ttaaacttct taaatacact
      301 aggaatccct tcaatattaa ttagcttatc ataagctata ggctctgcct tattttcata
      361 actaattcca tctataccac ttagcttatc actggcaaag taattatcct ttagcctatt
      421 agtctcatct aacctacctg caattgcccc taaatatgcc ttcccttcct ctatatctac
      481 tagactatag gacttttgta tttccttggc caggcctgaa attcctccta tataattgcc
      541 accttttaga ctacttctag aataggatga ataaatgggc cccatagatt ttccagcaat
      601 gcctcccaca tagttaccag ctgtactttc taccttggct gaagaaatta catctctaat
      661 aaagcctagg tccatatttc caactatacc acctacatgg ttctttttgc ccactatgtc
      721 tccatagttt tctgccttat caataacagc ccttgattca aagattgtat tggcggtaaa
      781 ccttccactt atatctatat catcctctgg atctagttcc agatctatgg acatggcccc
      841 tgctatgcct cctacatttc tgtctgcctc tatggtgcca tagtttctag tattatatac
      901 cttaccttcc ttcacctggt caatcctggt agttgataca tcttccacta tcctgtctag
      961 ttgcacttcc ctgttaaaaa tctcatctag aatatcaaac attagattaa caactcttaa
     1021 tagctggtta ctcatctcct ccatactatc cataaggagc ctaccttgtt tatccatatt
     1081 ttctagaaac tgccccagtg aattagatac atcttctata gactcaaata gcccatcctt
     1141 agcctgcaaa aaggtatcac caggtgtttc aaagtttggc ccatcaaagt ctttaattaa
     1201 ttccattatt tcctttaaag ccttggaaat atctttaaat tcttctgtag aatctcctag
     1261 aacttccata acctttgata tgatctctaa gccct
//
LOCUS       DATGSI010000334         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_400855, whole genome shotgun sequence.
ACCESSION   DATGSI010000334 DATGSI010000000
VERSION     DATGSI010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400855"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VK031_05195"
     CDS             <1..423
                     /locus_tag="VK031_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53346.1"
                     /translation="LHTHKSHIDDVAEITEIAHKAGLLCEAYITDNDTLGVNAGTDEE
                     LVQTIRDFEAVGVDMIGLCTGQTYKGQSATGFSDDFVRRMELFVKETNVIKILEGGIK
                     QGNIEAVKDIGFDILVISTAIDNYLEDKTIEIVAELNK"
     regulatory      673..854
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            991..1851
                     /locus_tag="VK031_05200"
     CDS             991..1851
                     /locus_tag="VK031_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HLS53347.1"
                     /translation="MKRKGILSIGILLILTMVLVACGQQEAPNGSGTESDGNGDKGTF
                     VVGLEADYAPFNWTQMDDSNGAVKMDGIAEYAGGFDVEIAKRIAEGLGKELVIKKIEW
                     DGLLPAVNSGVVDAIIAGMSPTEERAQEIDFTAPYYKSDLVMVVRKGGGYENATSIQD
                     FSGAKVAAQQATFHDTVIDQIEGVIHETPMANFSAMRVALESGIIDGYVTERPEAVSA
                     AAANENFTFVEFEDGFEASDEDIAVAVGLKKGSTELVEKINKILEEITEEERQEIMDQ
                     AIKNQPASEE"
     gene            1925..>2644
                     /locus_tag="VK031_05205"
     CDS             1925..>2644
                     /locus_tag="VK031_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HLS53348.1"
                     /translation="MSLKGAAEIAIKYWPMFYRGAYMTLLISIISTILGAILGLAIGV
                     IRTIPKPKKGGKRIALSIINGLLSAYIEFFRGTPMMVQSMVIYYGIAGAFGIQLNRTF
                     AAIFIVSINTGAYMAEIVRGGIVSIDQGQFEAAHALGMNHIQTMTNVILPQVIRNILP
                     ATGNEFVVNIKDTSVLNVISVTELFFQTKSIAGNTFKFFEPYFVTCIIYFIMTFTVTR
                     ILRYVEKRLDGPKNYNIIGNQM"
BASE COUNT          938 a          352 c          573 g          781 t
ORIGIN      
        1 ttgcatacac ataaatcaca tatagatgat gtggcagaaa taactgaaat agcccacaag
       61 gcaggtctac tatgtgaagc ttatattaca gacaatgata ccttaggtgt taatgctggt
      121 acagatgaag aattagttca aaccataagg gattttgaag ctgtaggagt agacatgatt
      181 ggactgtgta caggacaaac atataaggga caaagtgcca caggatttag tgatgatttt
      241 gtaaggagaa tggagttatt tgtaaaggaa actaatgtta ttaaaatttt agaaggtgga
      301 ataaagcagg gaaatattga agccgtaaaa gatataggct ttgacatctt agtaatatct
      361 actgcaatag acaactatct agaagataaa acaatagaaa tagtagcaga attaaataaa
      421 tagactaact aagacttaat ttgctgttgc taaaatttat ataaagctta ttccgaattg
      481 gcctcggctg atttggaata agttttttta cttagatttt tgtaatcatt tatttagata
      541 agattaatat actataatag atagatcatt cattatgtga tttgggaaaa agattctaca
      601 aaaacttgac aatcaaaaga ggtaatgtta tcatgttaga caataaaagt taataaaatc
      661 ctcatcctag ccgggtgagg tagaggagcg ataactaaga gtagctatat ggaggacgga
      721 atccagtgaa gtatagtgaa aggagttgtc gccgaaatcc tagataccta agtctagggt
      781 tgggattatg tctaataggc ataagactgt catcaaagta tccgctcgat actttgatgt
      841 gtgctatctc acattgagca agaaaggaga gaggaaaaaa gctttgcttt atgtgtatac
      901 gcacctgaca gtcttcaggt gcttttttaa ttattaaaat cagatcggat agcactttaa
      961 agaaaattta aagtaaggag aagatttgga atgaaaagaa aaggtatatt atctattgga
     1021 attttgttaa tcttaactat ggtattggtg gcctgtggtc agcaagaagc accaaatggt
     1081 tctggtacag aatcagatgg aaatggtgat aaaggcacct tcgttgtagg gctagaggca
     1141 gattatgcgc cttttaactg gactcaaatg gacgattcca atggggcagt aaaaatggat
     1201 ggtattgctg aatatgcagg tgggtttgac gttgaaatag caaaaagaat tgctgaagga
     1261 ttaggaaaag aattagtaat taaaaagatt gaatgggatg gcttgttgcc agctgttaat
     1321 tcaggagttg tagatgcaat cattgcaggt atgtctccta cagaagaaag ggcacaagaa
     1381 attgatttta cagctcctta ttataagtct gatttggtaa tggttgtaag aaaaggtgga
     1441 gggtacgaaa atgcaacttc catccaagac tttagtggag ccaaggttgc tgcccagcaa
     1501 gcaacatttc atgatactgt gatagatcaa atagagggag ttattcatga aacacctatg
     1561 gccaacttct cagccatgag ggttgcttta gaatcaggaa ttatagatgg ctatgttaca
     1621 gaaaggccgg aggcagttag tgccgcagct gcaaatgaaa actttacctt tgtagaattt
     1681 gaagatggct ttgaggcatc tgatgaagac atagcagtag ctgtaggact taaaaaggga
     1741 agtacggaac tagttgaaaa aattaataaa atattagaag aaatcactga agaagaacgt
     1801 caagaaataa tggatcaagc cataaaaaat caaccagctt cagaagaata ataagaactt
     1861 acctctaata ttcttctgaa atattagagg taagatattg tagcatttaa gaaaggggaa
     1921 caatatgagt ttaaaaggag cagcagaaat agcaattaaa tattggccca tgttttatag
     1981 aggggcttat atgactctac taatatcaat tataagtact attttagggg ccatattggg
     2041 cctggctata ggagtcatac ggacaattcc caagcccaaa aaaggtggca agcgcatagc
     2101 tttaagtata attaatggac tactttctgc ttatatagaa ttttttcgag gtactcctat
     2161 gatggttcag tctatggtta tatactatgg gattgcaggt gcctttggaa tacaattaaa
     2221 taggaccttt gcagccatat ttatagtatc tataaataca ggagcctata tggcagaaat
     2281 agtacgtggt ggtatagttt ctatagatca gggccagttt gaagctgctc atgctttagg
     2341 aatgaatcat atacagacaa tgactaatgt tattttgcca caggttatta gaaatatttt
     2401 gccggccact ggaaatgagt ttgttgtaaa tattaaggat acttcagtat taaatgttat
     2461 aagtgttact gagctatttt tccagaccaa atcaattgct ggcaatacat ttaaattttt
     2521 tgagccttat tttgtaacct gtataatata ttttataatg acttttactg ttacaagaat
     2581 tttaagatat gtagaaaaaa gattagacgg accaaaaaac tacaatataa ttggtaatca
     2641 aatg
//
LOCUS       DATGSI010000335         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_166236, whole genome shotgun sequence.
ACCESSION   DATGSI010000335 DATGSI010000000
VERSION     DATGSI010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166236"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2725
                     /locus_tag="VK031_05210"
     CDS             <1..>2725
                     /locus_tag="VK031_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLS53349.1"
                     /translation="GINGVASVTEIGILEEKIEVVLDQEKIDELNNKILDKVDSELLT
                     AEQELLKAQKEIEDGKVKLDKEEKSQLGQLVKAERAIDMGRDQISEAQLQITLGISKL
                     QENKELLQGAKEFIDGNEENLESLEKLREALAAIAVQLPEKNKGPVENILEKLDEVLS
                     TGESVNIGLSEIDDTIKTLEGQREELKNKKLELLDKEEEIKKGKALLSSEITKARIEL
                     TEGEKTLNEKLTELEEAKDEAFKQVSLDGVLTREMISNILMAQNFSMPAGYIKEEGTD
                     YLVKIGDKFDNIADIEKLLLFDTGEEAIGEIYLSDVAQVEERDNAGEIYAKVNGNNAI
                     ILNLQKQSNFSTSQVSKDINKKMKAIEAEHEGLTFTNLMDQGVYIDIVVDSVLSNIIY
                     GGILAILVLIVFLKDIKPTFIIAVSIPISIVFAIAMMYFTGVTINIISLAGLALGVGM
                     LVDNSIVVIENIYRLRNEGMGLVEAAVTGAKEVSGAILASTITTACVFLPIVFTKGIS
                     RQLFTDMGLTIAYSLFASLIVALTLVPVLGARVLDKTNSNQNKTLDKITKGYEKILAW
                     GLKNRAIVMISVVVLLGISGYMAYMMGTSFIPEMESHEMSLSIELPEGSSFDEFKKLA
                     DRITEKIIDIEGIHTVGAMQGGMLGDMTMGNRGDQTLSMYLLLDEERSVGNDHIEKEL
                     ISLGEDMGVEVFVNTSNMDMSQLGGTGIELLVKGREIDKLRDLALELEEIVKGTEGTT
                     DVNTSIGEGTEEIRIRVDKDKAMAEGLTVAQIFSHVNSVLDQDKSATTLTIANKDYPV
                     IVVNDKERSMAREDLKDMKITNDQGEEIKLGDLVSINEEKGLSSIYRASQERYITVNA
                     DIDIGYNIGLVSRDLERKIRDFELPEGYSIEFEGENKLIQDSLRDIGLMMVL"
BASE COUNT         1012 a          355 c          635 g          723 t
ORIGIN      
        1 aggaattaat ggagtagctt ccgtaactga gatagggatt ctggaagaaa agatagaagt
       61 agtactagac caggagaaaa tagatgaatt aaataacaaa atattagata aggtagattc
      121 ggaactgtta actgctgaac aggagctttt aaaagcccag aaggaaattg aagatggtaa
      181 ggttaaactt gataaagaag aaaaatcaca gctggggcag ttggttaagg ctgaaagagc
      241 tatagatatg ggcagggatc aaatttcaga ggcccagctt cagattaccc tgggtatttc
      301 aaaattacag gagaataagg aacttttaca gggggccaaa gaatttattg atggaaatga
      361 ggaaaattta gaaagcttag aaaagttacg agaggcttta gcagccatag ctgtgcaact
      421 gccagaaaaa aacaaaggac cagtagaaaa tatactagaa aagcttgatg aagttcttag
      481 cacaggagaa agtgtaaata ttggattaag cgagatagat gatacaatca agaccttgga
      541 agggcaaagg gaagaactta aaaataaaaa actagaacta ctagataagg aagaggagat
      601 taaaaaaggc aaggctcttt tatcctctga aataacaaaa gccaggattg aattaacaga
      661 aggtgagaaa accctaaatg aaaaactaac ggaactggaa gaagctaagg atgaagcctt
      721 taaacaggtc tccctagacg gagttttaac cagggagatg atatccaaca tattaatggc
      781 tcaaaacttt tccatgccgg caggctatat taaggaggaa ggaacagatt atttagtaaa
      841 aataggagat aaatttgata atatagctga tattgaaaag ctattattat ttgatacagg
      901 agaagaggca attggagaaa tttacttgtc agatgttgcc caggtggagg aaagggacaa
      961 tgctggagaa atttatgcta aggtgaatgg aaacaatgcc ataatattaa accttcaaaa
     1021 acaaagcaat ttctctactt cacaagtatc taaggatata aataaaaaaa tgaaggcaat
     1081 tgaagctgag catgaaggac ttacctttac aaatttaatg gaccagggag tatatattga
     1141 tattgttgtt gattctgtcc ttagcaatat tatttatggt gggatcctgg ccatcttagt
     1201 attaatagta tttctaaagg atataaagcc tacctttata atagctgtat caatacctat
     1261 tagtatagta tttgctattg caatgatgta ttttacaggg gttacaatca atataatctc
     1321 cttggccggt ttagccctag gtgtaggtat gttggtagat aactcaattg tggttattga
     1381 gaatatatat aggttaagaa atgaaggcat ggggcttgtg gaggcagcag ttactggtgc
     1441 taaggaagta tcaggagcta ttttagcctc aaccattaca actgcttgcg tgtttttacc
     1501 tatcgtattt acaaaaggta tttcaaggca gctatttact gatatgggac tgacaattgc
     1561 atattccttg tttgcatctc tgattgtagc tttaaccttg gtaccagttt taggggccag
     1621 agttttggat aagaccaact caaaccaaaa taaaacctta gataaaatta caaagggcta
     1681 tgaaaaaata ttggcctggg gattgaaaaa tcgagcaata gtcatgattt ccgtagttgt
     1741 tctactaggc atcagtggat atatggccta tatgatggga acctcattta ttcctgaaat
     1801 ggaatcacat gaaatgtctc tatcaataga attaccagag ggaagcagtt ttgatgaatt
     1861 caaaaaactt gctgatagga ttacagaaaa gattatagat atagaaggta ttcatactgt
     1921 aggagcaatg caaggcggaa tgctggggga tatgactatg ggaaatagag gggatcaaac
     1981 cctatctatg tacctactat tagacgaaga aagaagtgta ggtaatgatc atattgaaaa
     2041 agaattaata agccttggag aagacatggg ggtggaagta tttgtaaaca cctctaatat
     2101 ggatatgagt cagttaggag gcacaggtat tgagcttctg gtaaagggcc gtgaaataga
     2161 taagttaagg gacctagcct tggagcttga ggagatagta aaaggcacag agggtacgac
     2221 agatgtaaat accagtatag gtgaaggaac agaggaaata agaatacggg tagataagga
     2281 caaggcaatg gctgaaggct taacggtagc tcaaatattt agccatgtaa actctgtgtt
     2341 agaccaagat aaatcggcta caactttaac aatagctaat aaggattatc ctgtgattgt
     2401 agtcaacgat aaagaaaggt caatggcaag agaagactta aaggatatga aaattaccaa
     2461 tgatcaaggt gaagaaataa aactaggtga tcttgtatct ataaatgaag aaaagggtct
     2521 ttcatctata tacagggcat cgcaggaaag atatattaca gtaaatgcag atattgatat
     2581 tggttacaat ataggcttgg taagcagaga tttggaaagg aaaataaggg actttgaatt
     2641 accagaagga tatagcattg aatttgaagg tgaaaataag ctgattcaag attccttaag
     2701 ggatattggt ttaatgatgg tattg
//
LOCUS       DATGSI010000336         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_638707, whole genome shotgun sequence.
ACCESSION   DATGSI010000336 DATGSI010000000
VERSION     DATGSI010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638707"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VK031_05215"
     CDS             complement(<1..388)
                     /locus_tag="VK031_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HLS53350.1"
                     /translation="MDYKIKLDGYEGPLDLLIDLIEKEEVDIYDIPINKITKQYLAYI
                     YDMEELNLEIASEFLIMAATLLQIKSKMLLPKEKKIVEGEEVEIDPREELVKRILAYK
                     QFKLAAEELKELGNLELKAYYKPMEDL"
     gene            complement(398..985)
                     /locus_tag="VK031_05220"
     CDS             complement(398..985)
                     /locus_tag="VK031_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HLS53351.1"
                     /translation="MDYIYNIPGLLVAIVFHELAHGLTAYILGDDTAKKANRLTINPI
                     KHIDPIGFISMFIFGFGWAKPVPVNPLNFKRRKRDMLLVSLAGPLSNFLIAIITIFIF
                     NHVQISSPILARVLVLLLWYNIMLGIFNLLPFPPLDGSKILASLLPGRIEFYFYKYER
                     YLRLLLILLIFTNWIDKILSPIINAILDLLFIVLV"
     gene            complement(1051..>2183)
                     /locus_tag="VK031_05225"
     CDS             complement(1051..>2183)
                     /locus_tag="VK031_05225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HLS53352.1"
                     /translation="SIITIILILLLPLSSYGSIDIDAKSALLMDYNSGEIIYALNEHE
                     KLAPASITKVMTLLLTMEALNTNKIKLTDKVNISEHASQMGGTSVYLDAGEIQTVENL
                     IKAVSIRSANDASVALAEYIAGSEEAFVQLMNGKAKELGIKNTNFTNASGLPGENHYT
                     TAYDIGLMSRQLLRHKKIVEYLTIYMDDMEVGKSKASTQTMVNTNRLIKDYEGATGIK
                     TGYTSEAKHCISASAKKGDLQLIAVIMGADSSKVRFTEAAKLLDHGFSNFESITVGLK
                     GDVFVSIPVEKGNINQVDLMLERDSFILVPKGDQIKVEKKINHPEFLTAPIEPGTPLG
                     NVEVIVDGKIIDKVNLVAKSLVERGNIVNILKKVWANYLFNQ"
BASE COUNT          696 a          431 c          304 g          752 t
ORIGIN      
        1 ttaaatcttc cataggtttg taataggcct ttaactctaa attaccaagt tcttttaatt
       61 cttcagcagc aagcttaaat tgtttgtagg ctaaaatcct ttttactaat tcctctcttg
      121 ggtcaatttc aacctcttcc ccttcaacta tctttttctc ctttggcaac aacattttag
      181 atttaatttg taacaaggtg gcagccataa ttaaaaattc actggctatc tctaaattta
      241 attcctccat atcataaata taagccaaat attgtttggt aattttattt atagggatgt
      301 catatatgtc aacttcttct ttttcaatta aatctattag tagatctaaa ggtccttcat
      361 atccatctaa ttttatttta taatccataa cctactccta tactaaaaca ataaataaca
      421 aatctaaaat tgcattaata attggactta atattttatc tatccaatta gtaaagataa
      481 gtagaattaa taataatctc aaatatcttt catacttata aaaatagaat tcaatccttc
      541 ctggtaatag gctggccaag atctttgaac catccaaagg tggaaagggc agaagattaa
      601 atattcctaa catgatattg taccacaaaa gtaatactaa aactctagcc aggatagggc
      661 tagatatttg tacatgattg aaaataaaaa tagttataat tgctattaga aaattactta
      721 agggacctgc caaggatacc agcagcatat cccttttcct tcttttaaaa tttaacggat
      781 ttacaggaac aggcttggcc catccaaaac caaaaataaa catagagata aaccctatag
      841 gatctatatg ttttataggg tttatcgtta atctatttgc ttttttagcc gtatcatcac
      901 ctagtatata tgctgtcaat ccatgggcta attcatgaaa tacaatggcc actaataagc
      961 ctggaatatt ataaatataa tccatacaac accttccata tttactacta ttatacaagc
     1021 tctttcgctt aaagtcgagg tatatctttt ctactgatta aatagataat tagcccatac
     1081 cttttttaaa atatttacaa tatttcccct ttcaaccaaa gatttggcca ctaagttcac
     1141 cttatctata atcttcccat caactataac ttccacatta cctaatgggg tccctggctc
     1201 aataggggcc gttaaaaatt caggatggtt aatctttttc tcaaccttta tttgatcccc
     1261 ctttggaacc aagataaagg aatctctctc caacattaag tcaacctggt ttatattccc
     1321 cttctccaca ggaatactta caaagacatc tcccttaagt cctacagtta ttgattcaaa
     1381 gttagaaaaa ccatgatcta ataatttagc tgcctctgta aaccgcacct tagaactatc
     1441 agctcccata ataactgcga ttaattgaag atcacctttt ttagctgaag cagatataca
     1501 atgtttagcc tcacttgtgt agccggtctt aataccagtg gcaccttcat aatccttaat
     1561 taacctattt gtattgacca tggtttgagt actggccttt gatttcccta cttccatatc
     1621 atccatatag atagtaaggt attctacgat ttttttgtgc cttagtagct gcctggacat
     1681 aaggcctata tcataggctg tagtatagtg attctctcca ggtagtccgc tggcattagt
     1741 aaaattagta ttttttatac ccagttcttt ggcctttcca ttcataagct gcacaaaggc
     1801 ttcctctgaa ccggcaatgt attctgccaa ggctacagag gcatcgttgg ctgaccttat
     1861 agatacggcc tttattaaat tttcaactgt ttggatctct ccagcatcaa gataaacaga
     1921 ggtgccaccc atttgagagg catgctcact tatatttacc ttatcagtca actttatttt
     1981 attagtattt aaggcctcca tggttagcaa aagggtcata acttttgtaa tactggctgg
     2041 tgccaatttt tcatgttcat ttaaagcata gataatttca ccagaattgt aatccataag
     2101 tagggctgat ttagcatcta tatctatact tccataacta gacaagggaa gaagtaggat
     2161 taaaataatt gttataatgg ata
//
LOCUS       DATGSI010000337         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_21153, whole genome shotgun sequence.
ACCESSION   DATGSI010000337 DATGSI010000000
VERSION     DATGSI010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21153"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..851)
                     /locus_tag="VK031_05230"
     CDS             complement(180..851)
                     /locus_tag="VK031_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HLS53353.1"
                     /translation="MRKFLSILFITVGIVLISLPFLSNKYLEYKIKANQDILEDISAE
                     ELKKNESIEAEYDYSLIDDIDPSLLFSEMEKNYGESMIGQIVIADLGINLPIMKGTTN
                     SNLLAGATTMRPDQKMGQGNYPLAGHYARNKSVLFASLLDIEKDTIVKITNKETIYEY
                     KIYDVIIVPDTSIHLIEDKLAEDRGRPVISLMTCYYTSKNGKRFFALGELVDEYDYAP
                     EKLIE"
     gene            complement(947..2053)
                     /gene="rodA"
                     /locus_tag="VK031_05235"
     CDS             complement(947..2053)
                     /gene="rodA"
                     /locus_tag="VK031_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HLS53354.1"
                     /translation="MFNIKKKSFKQFDFILFAIVLILCGFGLLMIKSATLSMGTSRLV
                     KTQAIAMILGFIGMVILALMDYQFLGKLYIPIYVVCILLLLATIKWGFGMEQWGADSW
                     LSIGGVAFQPSEFVKVGLIISLAKFIDNNKAKINEPVTLLKILAFAFLPVYLILKQPD
                     AGTAMVFVFFIAIMLFVAGIDWKYVGYAFIAGLASLPLLWLKLGTYQMNRIFDFLHPE
                     RDPTGTGYQALQGKIAIGSGKIFGRGLFNGVFTQYDYIPIKESDFIYSVIVEELGFIG
                     GIVLIILFFVFLIRLIRISKNCEDLFGATMVMGFAAMFVFHIWENIGMTMGLMPVTGI
                     PLPFISHGGTFILVSMASVGIALSVGMHREGLNF"
     gene            2163..>2513
                     /locus_tag="VK031_05240"
     CDS             2163..>2513
                     /locus_tag="VK031_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLS53355.1"
                     /translation="MLKIGSHLSISNGFYKAALDALSIGANTFQFFTRNPRGGRARDL
                     DPNDIKKLHEIMNKYKFAPLFAHGAYTMNLASDKKHTRDFAKRIFKDDLKRLEKISNA
                     YYIFHPGSHVGQGSK"
BASE COUNT          878 a          479 c          396 g          760 t
ORIGIN      
        1 agatgaaagg cttttcctaa tggggggata atcatgaagg ggaaaaagac aaatatatac
       61 ttaaccatag ccatatgttt acttattata gccatatcta gcctgtatat ttatatgata
      121 aattttcaaa ataaaaagca aaaataaacc ctaaatcctt ttggatttag ggtttttatc
      181 tattctatta acttttcagg ggcataatca tattcatcta ccaattctcc tagagcaaaa
      241 aacctttttc catttttaga agtataataa caggtcatca aagaaatcac tggccttccc
      301 ctatcttcag ccaatttgtc ctctattaaa tgtatagatg tatctggcac aataattaca
      361 tcataaatct tatattcata gatggtttcc ttgttagtta ttttaactat agtatctttt
      421 tcaatatcta ggaggctggc aaataggacg cttttgtttc tggcataatg accagccaag
      481 ggatagttac cttggcccat cttttggtct ggcctcatgg tagtagcccc ggccaataga
      541 ttagaattgg ttgtcccctt catgataggc aggtttatgc ctaggtcagc tatgacaatt
      601 tgacctatca tagattctcc atagtttttc tccatctcag agaaaaggag acttggatct
      661 atatcatcta ttaaggaata atcatattca gcttctatag attcattttt ctttaattcc
      721 tcagcagaaa tgtcttccaa tatatcttga ttagccttaa ttttatattc taaatatttg
      781 ttgctaagga aaggtagact aataagaact ataccaactg taataaataa gatggataaa
      841 aactttctca taataaatta ctcccatata tagaactaaa atttaatcta ctaaaaaatt
      901 cttttacttt ctacagaagg ccaaggaaaa ataatgtatc caggtattaa aagtttaatc
      961 cttctctatg catacctaca cttaaggcaa tacccactga ggccatactt accaaaatga
     1021 aggttcctcc gtgactaata aagggaaggg gtattcctgt aacaggcatt agccccatgg
     1081 tcatacctat attctcccat atatgaaata caaacatggc cgcaaaaccc ataaccatgg
     1141 tggcgccaaa gagatcttca cagttttttg atatcctgat taatctaatt aaaaatacaa
     1201 agaatagtat aattaaaact ataccaccga taaatcctaa ctcctccact ataactgaat
     1261 agataaagtc actttcttta attggaatat aatcatattg ggtaaatact ccattaaaaa
     1321 gaccccgtcc aaagatcttt ccagaaccaa tggcgatttt tccttgcagg gcctggtaac
     1381 cagtacctgt agggtccctt tcaggatgta aaaagtcaaa tatcctattc atttggtagg
     1441 tacctaattt aagccacaga agaggcaagg acgctaatcc ggcaataaag gcatatccaa
     1501 cgtacttcca gtcaatacct gccacaaaca acataatggc tatgaaaaag acaaaaacca
     1561 tggctgttcc agcatcaggt tgcttcaaga taaggtatac tggcaaaaag gcaaaggcca
     1621 agatctttaa taatgtgact ggttcgttga ttttagcctt attattatca ataaatttag
     1681 ccaaggaaat aatcaaacct accttaacaa attctgatgg ttggaaggca acaccaccta
     1741 tagacaacca ggaatctgcc ccccactgct ccataccaaa accccatttg atagtggcca
     1801 aaagtaaaag aatacaaacc acatatatag gtatatataa tttacccaag aactggtaat
     1861 ccattagggc caggataacc atgcctataa agcctagtat catggcaatg gcctgggttt
     1921 taaccaatct agaggtaccc atacttaagg tggccgattt aatcattaat agtccaaagc
     1981 cacacaggat gagcactata gcaaatagga taaagtcgaa ttgcttaaaa gacttcttct
     2041 taatattaaa catggaatac ttcctttctc tatactaaaa ttgattatat catatattaa
     2101 taaatggctg tagttaaaat tatttacaat gtggtatcat tatagataca aggagtgatt
     2161 taatgctaaa aataggtagc catttatcca tatcaaatgg cttttataag gcagctttag
     2221 atgccctatc tataggtgct aatacatttc aattttttac cagaaaccca agaggaggca
     2281 gggccagaga cctagatcct aatgatataa aaaaattaca tgaaattatg aataagtata
     2341 agtttgctcc cctttttgcc catggggctt atactatgaa cttggcatct gacaaaaaac
     2401 atacaagaga ctttgccaaa agaatcttca aagatgattt aaaaaggtta gaaaaaattt
     2461 ctaacgcata ttatatcttt cacccaggct ctcatgtggg gcaaggtagt aaa
//
LOCUS       DATGSI010000338         4879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_69953, whole genome shotgun sequence.
ACCESSION   DATGSI010000338 DATGSI010000000
VERSION     DATGSI010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69953"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2018
                     /locus_tag="VK031_05245"
     CDS             <1..2018
                     /locus_tag="VK031_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLS53356.1"
                     /translation="FVIMGVIFIATSLAIFQIFLTQIKRRSRKLVLLRAIGATKRQII
                     GIILYEGLYFLTRGLIIGVPIGFISVLSLLYGMNRIGGRDLKFYVDIRLLIPGLIAGI
                     LSLFLGMIIPMIYAVRIPLVGSMNRPSRSKSSYNKKRSTSYQSFNRINWEYIKANRGK
                     TLLSFGISFVTITILLSSLFLCYYSFDNYKKLVLKPGRPDYALETYYGESYRKLLEQK
                     KELLNIDGVENAYAYKVGRELHFWYEDINNNEMLKDFDKLLPADLRKVYFTKHNSNLK
                     KMEVEPFISDAFLSKIYGIDPDSDLFKKYESFLTRGNIDREKFNAGEEVIILSPMYIK
                     GEKNSIDNINLNKNTILDLTNEDNRMSWIFENTGSYKLSYDKQDSNHYIKQDFLKVGD
                     RIQLSSSKEEVTAQSYESSFVTKQFKVAGIIDYFPQGSQWPFYHSKANYIVISSIRAM
                     EDIYPFTVRGLGNFEPASMKAAVDTIYPNRYGRTLWYIDTDSKKQDVVLDSKLLAYAH
                     NNNYTIYNYKESNAKLYQESFNNALIISLLGITASAIAFIILYNILESQMEQDRNRIG
                     ILQALGVTKKEFSTYYIKLGLINGILALGLINIILMTIMLISSNKNMHLLDRIKDALI
                     YRLWLYPWLVHILLSVAFLSLTVLIYYLPARKVIENSPVENIRSLSR"
     gene            2069..2794
                     /locus_tag="VK031_05250"
     CDS             2069..2794
                     /locus_tag="VK031_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HLS53357.1"
                     /translation="MKNIAAFFDIDGTIFRNSLMIEHFQKLINFEVIDPAIWYTKIKK
                     VYSEWESRFGDFEEYLEKLAEVYIEELKGINRSYIEFIATHVIEVNGDMVYKFSREQI
                     EWHKKQNHKIFFISGSPDFLVSKMAEKYEVTEYRGTLYKVDENNKFTGEIEKMWNSEN
                     KELVINEFIEKYKVDLENSYAYGDTTGDLSMLKMVGNPVAINPNRELVDVIKADKELS
                     KKATIVVERKNVIYKLDSTVKVL"
     gene            2881..3927
                     /locus_tag="VK031_05255"
     CDS             2881..3927
                     /locus_tag="VK031_05255"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HLS53358.1"
                     /translation="MKKIILAILVALTVALFVACAPELKGEEDMDAEMDLDSVLYDYY
                     PFQENKVYEYIGIGSEFAEETIYYEFIDNNKAQLKIQNPGTTVVKIIEISQGMVREIY
                     SEGEFYHIEDILDLEGDREEVLLKEPIRLRNSWPDSEGNTREITGVDVDIETPYGSFK
                     ALEVSTDINEATVKEYYAKGIGLVAHIYKDGDFEIKSLLKSIKEGPISSPIRLYYPSE
                     EGELVFIDDKINFSTNQEVERLLEEAMKRPPKRKLRPLINEDTKINSIYLDRNLWLLK
                     VDFSQELLAEDEEKSALDDKLLLGIVNTLGYYYDVDKVAITVEGIPIKEQSHLPREDG
                     AFKVDLENIKEYKQ"
     gene            complement(4150..>4879)
                     /locus_tag="VK031_05260"
     CDS             complement(4150..>4879)
                     /locus_tag="VK031_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLS53359.1"
                     /translation="IQDIIDFMSFAQDTSNMEIYERIFYKKKGFISRRQINYAKTLNH
                     NLSVFQRIKDFPGINSFYERNLNQLDLDFKKLSKLKPKDAISFIEYDLEYDQYLRDNS
                     MKFGYTYDNLVTILYFLKMMAEKTNNLGQLISRLNHVKSICEKPPYKNKGVTLSTIHS
                     AKGLEFERVYMIDLIDGEFPNYTSIEKFLTGNPNLMEEERRLFYVGMSRAKSELNLLT
                     VKYINGRSVSPSRFLNELEKITNS"
BASE COUNT         1749 a          632 c          897 g         1601 t
ORIGIN      
        1 gttttgttat tatgggagta atttttatag ctacttctct agctattttt caaatatttt
       61 taactcagat aaaaagacga tccaggaagt tagttttgct aagagccata ggggctacta
      121 aaagacaaat tataggtata atattatatg aaggtttata ctttttaacg agaggtctaa
      181 taattggagt tcctataggg tttatatctg tattgtcctt attatatgga atgaatagga
      241 ttggaggtag agatctaaag ttttatgtag atataagact actgattcca ggattaatag
      301 ctggcatttt atctctattt ttgggtatga taattcctat gatatatgca gtaagaatac
      361 cattggtagg tagtatgaat agaccctcca gatctaagag ctcatataat aaaaaaagaa
      421 gtacatcata ccaaagcttt aacaggataa actgggaata tataaaagca aatagaggaa
      481 agactttatt aagttttggc ataagttttg taactattac catacttttg tcttcattat
      541 ttctttgtta ttattctttt gataactata aaaagctagt tttgaaacct ggtagacctg
      601 attacgcatt agaaacttat tatggagagt cttatagaaa gcttcttgaa caaaaaaaag
      661 aactattgaa catagatgga gtggagaatg cttatgctta taaggtagga agagaacttc
      721 atttttggta tgaagatatt aataacaatg aaatgttaaa ggattttgat aaattattac
      781 cagctgattt aagaaaagta tattttacta agcataattc aaacttaaaa aaaatggaag
      841 tagaaccctt tatatctgat gcttttttaa gcaaaatata tggtatagat ccagatagtg
      901 atttgtttaa aaaatatgag agcttcttaa ctagaggtaa tatagataga gaaaaattta
      961 acgcaggcga ggaagtaatt attctttcac ctatgtatat aaaaggagaa aaaaatagta
     1021 tagataatat taacttaaac aaaaacacca tactagattt aactaatgaa gataatagga
     1081 tgagttggat ttttgaaaat actggaagtt ataagttatc ctatgacaag caagatagta
     1141 atcattatat aaagcaagac tttttaaaag taggagacag aattcagctt tcttcaagta
     1201 aagaagaagt aacagcacaa agctatgaat cttcttttgt tactaagcag tttaaggttg
     1261 ctggaataat agattatttt ccacaaggta gccagtggcc cttttaccat agtaaggcaa
     1321 attatattgt catatcatct attagagcta tggaggatat ttatcctttt actgtaagag
     1381 ggcttggtaa ttttgaacca gccagcatga aggctgcagt agatacgata tatccaaata
     1441 ggtatggcag gaccttatgg tatatagata cggattcaaa aaaacaggat gtagtgttag
     1501 attcaaaatt attggcttat gcacataaca ataattatac catttacaat tataaagaaa
     1561 gtaatgcaaa attgtatcaa gaaagtttta ataatgcttt aataatatcc ttattaggaa
     1621 taacagcttc agcaattgcc tttattattc tttataatat tttagaatca caaatggagc
     1681 aggatagaaa taggataggt atacttcaag ctttaggagt taccaaaaaa gaattctcaa
     1741 cttattatat aaaattaggt ctgattaatg ggattcttgc cttaggacta attaatataa
     1801 ttttaatgac aattatgctt ataagttcta ataaaaatat gcacttatta gataggataa
     1861 aagatgctct gatctataga ttatggctat atccttggct agttcatatt ttattatcag
     1921 ttgctttttt aagtctaaca gttttaatct attatttgcc agcaagaaag gtaattgaaa
     1981 attcaccagt ggaaaatatt agaagcttaa gtagataaaa ttttaaaata gaaccaagct
     2041 ttggttttga aaaggattag gtgattatat gaaaaatata gctgcatttt ttgacattga
     2101 tggaaccatc tttagaaatt ccctaatgat tgaacacttt caaaagctta taaattttga
     2161 agtcatagat ccagctattt ggtacacaaa aataaaaaag gtatattcag aatgggaaag
     2221 taggtttggt gattttgagg agtatttgga aaaactggca gaggtttata tagaagaatt
     2281 aaagggaatt aataggtctt atatagaatt tatagctacc catgttattg aagttaacgg
     2341 agatatggtt tataaatttt ctagggagca aatagaatgg cataaaaagc aaaaccacaa
     2401 gatatttttt atatcaggta gtcctgactt tctagtatct aagatggcgg aaaaatatga
     2461 ggtaacagaa tacaggggaa ccttatataa ggtagatgaa aacaataaat ttactggcga
     2521 aattgaaaaa atgtggaact cagaaaataa agagctggtc ataaatgaat ttatagaaaa
     2581 atacaaggtg gatcttgaaa acagctatgc ctatggagat acaactgggg atttatctat
     2641 gttaaagatg gttggaaacc cggtggccat aaaccctaat cgggaactgg ttgatgtaat
     2701 taaagcagat aaggaactat caaagaaggc tactattgta gttgaaagaa aaaatgtaat
     2761 ctataaattg gattcaacag taaaagtttt ataaattact gtaatctaat cgtaaacaat
     2821 agtggtgaca tcataggtat aattaaatat agtacaattc ttgtaacaaa ggtgggaaaa
     2881 atgaaaaaaa ttatacttgc cattttagta gcactaacgg ttgccttatt tgtagcctgt
     2941 gcaccagagt taaagggaga agaagacatg gatgcggaaa tggatttaga ttcagttctt
     3001 tatgattatt atcctttcca ggaaaacaag gtctatgaat atataggcat aggcagtgaa
     3061 tttgctgaag agacaattta ttatgagttt atagataata acaaggccca actaaaaata
     3121 caaaatcctg gcactacagt tgtaaagata atagaaatca gccagggtat ggtaagggaa
     3181 atatattcag agggagagtt ttaccatata gaagatatct tggatttgga gggggataga
     3241 gaagaggttt tactaaaaga acctataagg ctgagaaatt cctggccaga ttcagaaggt
     3301 aacacccgag agatcacagg tgtggatgtg gatattgaaa ccccctatgg aagttttaag
     3361 gccttggagg taagcacaga tataaatgag gctacggtga aagaatacta tgctaaaggc
     3421 attgggctgg tggctcatat ctataaggat ggcgactttg agataaaaag cttactaaag
     3481 tctatcaagg agggccctat aagtagcccc ataagattat attatccttc agaagaagga
     3541 gaactggtct ttatagatga taagataaat ttctctacta atcaagaagt agagaggctt
     3601 ctggaagaag ctatgaaaag gcctcctaag agaaagctta gaccattgat taatgaagat
     3661 actaaaatca actcaatcta tttagataga aatctatggc tattaaaggt tgacttttca
     3721 caagaattat tagctgaaga tgaggagaaa agtgctttag atgataagct tttattagga
     3781 atagtaaata ctctaggata ttactatgat gtggataagg tggctataac agtagaaggt
     3841 atccccataa aagaacaatc ccatttgcct agggaggatg gggcctttaa agtagacttg
     3901 gaaaatatta aggaatataa acagtaaaag gacctaagcc aaacaggtcc ttttctgtct
     3961 ttcctatggt taattttatt atctttgttg tttttgttgt ctttcttgtc tttcttgttt
     4021 ttctctttct tgttgctgct gttgttgctg tttttgcttt ttacttcttt gtttttccat
     4081 ttctaccctc ctaaatgcat gttataaaag actgtgcctt aatagtattt acctttttat
     4141 attttttagt caagaattag tgattttttc taattcattt aagaaacggg agggagaaac
     4201 acttcttcca tttatatatt ttactgtcaa aagattcagc tcactttttg cccggctcat
     4261 acctacataa aatagccttc tttcttcttc cataaggtta ggattaccag ttaaaaactt
     4321 ctcgatactg gtgtagtttg gaaactcacc atcaattaaa tctatcatat agaccctttc
     4381 aaactccaat cccttggcag aatggatggt agatagggta actcctttat ttttataggg
     4441 aggtttttca caaatagact taacatggtt taggcggctg attaattggc ctaaattatt
     4501 agttttttca gccatcattt tcaaaaaata taagatagta accaagttat cataggtata
     4561 tccaaatttc atagaattat cccttaaata ctggtcatat tccaggtcat actcaataaa
     4621 agatatagca tctttaggct ttaatttgga caacttttta aagtctaaat ccagctggtt
     4681 taaatttctt tcataaaagc tattaatacc tgggaaatct tttattcttt gaaagactga
     4741 taaattatga tttaaggtct tggcatagtt aatttgccgc cttgaaataa agcccttctt
     4801 tttataaaat attctttcat atatttccat atttgatgta tcctgggcaa agctcataaa
     4861 atcaataatg tcttggatg
//
LOCUS       DATGSI010000339         4393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_304707, whole genome shotgun sequence.
ACCESSION   DATGSI010000339 DATGSI010000000
VERSION     DATGSI010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304707"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1948
                     /gene="pknB"
                     /locus_tag="VK031_05265"
     CDS             143..1948
                     /gene="pknB"
                     /locus_tag="VK031_05265"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HLS53360.1"
                     /translation="MIGLTLGDRYEILEEVGQGGMATVYKAKCKLLNRYVAIKILKEE
                     FIDDKNFMKKFKRESQAAASLSHPNILNVYDVGAETINEKNIPYIVMEFIEGKTLKQI
                     IVERGRLTVDETIYYSRQIAEAIKHAHINNVIHRDIKPQNIMITKDNRVKVTDFGIAR
                     AASSSTLTVSSNVLGSVHYFSPEQARGGYTDETSDIYSLGITMYEMVTGKLPFTGENL
                     VSVALKHVQEEAVSPRELNKDIPSQLEAIILKCIQKNQGDRYPSLSALITDLKGIELF
                     GADIPEDNLNDESPTRIIPTEEIDNLVENSEEPLGKKKKKDNNMKVTLLAIGLAFLLV
                     STLFFGYFRLKGLFKVEEVTVPFILGMEEEEARQEIEDLGLEFKVVGLVKSEEFEPGQ
                     VASQSVEAGTKVRKGFTIEVTISEGQDMVRVPSLINKSLEEAQADLAELGLSIGTVSY
                     RPSDVTPKDLIIKQEPEANTYLEPGSKINVIISEGEEIRKVIMPRLIGQNIIDAKNAL
                     LSLDLTIGDVIEQYSDSVETDKVIWQSIESGTELETKTKVDLYVSKGPEKKPDEQEPT
                     EPEDDKDKGQEEKPEEEEEKDKDQDKDKDEEGEKD"
     gene            1981..2856
                     /gene="rsgA"
                     /locus_tag="VK031_05270"
     CDS             1981..2856
                     /gene="rsgA"
                     /locus_tag="VK031_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HLS53361.1"
                     /translation="MVKGRIIKGVGGFYYVKTKDGIIESRARGVFREDKLKPLVGDRV
                     KIKVSDEDNSGYIIEIEDRQTELLRPPVSNVTQAIIVMSIKSPDINTWLLDKFLLMAD
                     YEGLDILICINKCELDEEFAIKIEKIYKEAGYKVLLTSAALGLGIEELEEVLNGSISV
                     LAGPSGVGKSSLLNRINPDFNLDTGDVSARTRRGRHTTRHVELLELKDNTFVLDSPGF
                     SSLDLKFIENESQVRNYFKEINKYGFQCAFHSCLHDREPNCAVKAYVEAGKISQERYK
                     NYLSILNEVRNIRRY"
     regulatory      2907..3007
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3065..3607
                     /gene="thiT"
                     /locus_tag="VK031_05275"
     CDS             3065..3607
                     /gene="thiT"
                     /locus_tag="VK031_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HLS53362.1"
                     /translation="MKKRWNVRMMTEGGLMIALTALLNHITIYQAPQGGSVSAGGMVP
                     LLLFAIRWGLGPGFVVGATYGLLDFILKPHFYHPVQLILDYPLGYGLLALAALAYKVK
                     KGKVQEYINLIIGIALGLGGRMICHVLSGVIFFRDAAGDQNPWIYSIIYNSTYLVPEF
                     LISVLVFLLIWKPVRNSIQR"
     gene            3619..4254
                     /locus_tag="VK031_05280"
     CDS             3619..4254
                     /locus_tag="VK031_05280"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HLS53363.1"
                     /translation="MKGLIVSSGDINDYQLLANIIKKYDYVVCADGGIRHLKNLGYIP
                     DLVVGDLDSTKRLDLTYIEENSIKIKKFPTVKDSTDTELALYHLIEVGFKEIDLIGVT
                     GSRLDHTFANLLLLRILTRNNITGKIINDTNQVYHIKDFIKLEKKDYSYVSIIPLNHD
                     GVLVSLNGFFYSGAKQLIPYGTTLGVSNKLIEDEGYIKVHKGEALIIESKD"
BASE COUNT         1607 a          586 c          966 g         1234 t
ORIGIN      
        1 gataaggaaa tatttgaaat aataaaggat gcaaaaaacg taaaagatgc ttgtgaaggc
       61 ttaattgtta aggctaaaga aaatggtggc ctagacaatg taactgtaat tatgattaaa
      121 tttaagtaaa cggggtgaaa aaatgattgg actgactttg ggcgatagat atgaaatact
      181 tgaggaagta ggccaaggtg gcatggccac agtatataag gccaaatgca aattactaaa
      241 taggtatgta gcaataaaaa tcctaaagga agagtttata gatgataaaa actttatgaa
      301 aaagtttaaa agggagtccc aagcagctgc aagtctttcc catccaaata ttttaaatgt
      361 atatgatgtg ggagcagaaa caataaatga aaaaaatatt ccctatattg ttatggaatt
      421 tatagaaggg aaaactttaa aacagatcat tgttgagaga ggtaggttaa ctgtagatga
      481 aactatatac tacagcagac agatagctga ggctataaag catgcccata taaataatgt
      541 tatacatagg gacattaagc cacaaaacat tatgattact aaggataaca gggtgaaggt
      601 gactgacttt ggaatagcca gggcagcaag ctcttctacc cttacagtat cttccaatgt
      661 attgggatct gtccattatt tctctcctga acaggcaaga ggaggatata cggatgagac
      721 ctcagatatt tattctttag gcatcaccat gtatgaaatg gttacaggaa agctaccctt
      781 tacaggtgaa aacttagttt cagttgccct taaacacgta caggaagaag cagtttctcc
      841 tagggagcta aataaggata taccctctca attggaggcc ataatattaa aatgtattca
      901 gaagaaccaa ggggatagat atcctagttt atctgcctta attactgatt taaagggaat
      961 agaattattt ggggcagata taccggaaga taacttaaat gatgagtcac ctaccagaat
     1021 aattccaact gaagaaattg acaatttggt tgaaaacagt gaagaacctt tgggaaagaa
     1081 aaagaagaaa gataacaaca tgaaggttac cctattggca ataggtttag catttttatt
     1141 agtttctact ttgttttttg gatactttag actaaaagga ctctttaagg tggaggaagt
     1201 cactgttccc tttatattag gtatggagga agaagaggca agacaggaaa ttgaagattt
     1261 ggggcttgaa tttaaggttg taggactggt gaaaagtgaa gaatttgaac ctggtcaagt
     1321 ggccagtcaa agcgttgagg ctggaactaa ggtgagaaaa ggattcacaa tagaagtaac
     1381 cattagtgaa ggtcaggaca tggtaagggt gcctagtctt ataaataaat ccttagaaga
     1441 agcccaggca gacctagccg aattaggatt atcaataggt acagtaagct ataggccttc
     1501 tgatgttaca ccgaaagacc taattataaa acaagaacca gaagctaata cttatttgga
     1561 gcctggttca aagattaatg tgataataag tgaaggggaa gaaatcagaa aggttattat
     1621 gccaaggctg ataggccaaa acataataga cgccaagaat gctcttttat cattggactt
     1681 aaccatagga gatgtaatag agcaatatag tgattctgta gaaacggata aggttatttg
     1741 gcaatccatt gagtcaggaa ctgaattgga gactaaaact aaggtggact tatatgtaag
     1801 taaagggcca gaaaagaaac ctgatgaaca ggaaccaaca gaaccagaag atgataagga
     1861 taaggggcag gaggaaaagc cagaagaaga agaggaaaaa gacaaggatc aagataagga
     1921 caaggatgaa gaaggggaga aggattaagt attagaaaaa ataaaaagta gtggtgattt
     1981 atggtaaaag gtagaataat taaaggtgta ggtggatttt attatgtaaa gacaaaagat
     2041 ggaataatag aaagtagggc cagaggcgtt tttagagaag ataaattaaa gcctttagtt
     2101 ggagacaggg taaaaataaa ggtcagtgat gaggataatt ctggttacat tatagagata
     2161 gaggataggc agacagaatt attacgacca ccagtttcta atgttactca ggcaattata
     2221 gtaatgagta taaaaagccc tgatataaat acctggcttt tagataaatt tttgcttatg
     2281 gcagattatg aaggattaga catactaatt tgcataaata aatgtgaatt agatgaagaa
     2341 tttgctataa aaattgagaa aatttacaag gaagcaggat ataaggtttt gctaactagt
     2401 gcagccctgg gattaggtat agaagagctt gaagaggtct taaatggaag catcagtgtc
     2461 ctggccgggc cttctggggt aggaaaatca agcttattaa ataggataaa tcctgatttt
     2521 aatttagaca ccggcgatgt aagtgccaga acaagaaggg gtagacatac caccagacat
     2581 gtggaattat tagagctaaa agataatacc tttgtactag attcgccagg ttttagttcc
     2641 ttagatctga aatttataga aaatgaaagt caggtaagaa attattttaa ggaaataaat
     2701 aagtatggat ttcagtgtgc ctttcactcc tgcctacatg acagggagcc taattgtgct
     2761 gtcaaggcct atgtggaggc tggtaaaatt tctcaagaaa gatataaaaa ctacctatcc
     2821 attcttaatg aagttagaaa tattcggaga tattaaattg aatatatcct tataatatga
     2881 taaactattt agtacaattg aatagtcata aacactaggg gggccaaaaa ggctgagaga
     2941 ggactagtcc cgacccttat aacctgacct aggtaatact agcgtaggga agtgagtata
     3001 ttttgttttc tatacccatt acctatgtta ggtaatgggt ataattttta taggaggaat
     3061 gtagatgaaa aaaagatgga atgttagaat gatgactgaa ggtggattaa tgatagcttt
     3121 aacagcttta ttaaaccata tcaccatata tcaggcacca caaggtggat cagtatctgc
     3181 tggtgggatg gttcctttac tcttgtttgc tatacgttgg gggttagggc ccggttttgt
     3241 ggtgggagca acttacgggc tcttagactt tatcctaaag ccacactttt accatccagt
     3301 tcagctaatt ttagattatc ctttaggcta tggattgtta gctttggctg cattggccta
     3361 taaggtaaaa aaaggtaaag tacaagagta tataaattta atcataggga ttgctctagg
     3421 tctaggtggg agaatgatct gtcatgttct atcaggcgtt attttcttta gagatgcagc
     3481 aggtgatcaa aatccttgga tttattcaat catttataat tcaacttatt tagtaccaga
     3541 gttcttaata tctgtacttg tttttttatt aatttggaaa ccagtaagaa actcaataca
     3601 aagataggaa ggtaaattat gaaagggtta attgtctcta gtggagatat taatgactat
     3661 caattgctag ctaatataat aaaaaaatat gactacgttg tttgtgcaga tggtggtatt
     3721 aggcacttaa aaaacttagg ctatattccc gatcttgtag taggtgattt ggattcaact
     3781 aaaagattgg atttgaccta tatagaagag aatagcataa aaattaagaa attcccaaca
     3841 gttaaagata gcacagatac ggaactggcg ctgtaccatc taatagaggt gggctttaag
     3901 gagatagatc ttataggtgt aacaggtagt agattagacc atacttttgc taatttacta
     3961 ttactaagaa tccttacaag aaataatatc acaggtaaga taatcaacga cactaatcaa
     4021 gtataccata taaaggattt tataaaactg gaaaaaaagg attactccta tgtttcaata
     4081 ataccattaa atcatgatgg agtcctggtt agtttaaatg gatttttcta ttcaggggca
     4141 aaacaactta ttccttatgg aaccacctta ggggtaagta ataaattaat agaagatgaa
     4201 ggttatataa aggttcataa aggggaggcc ctaataattg aatcaaaaga ttagataaat
     4261 tttagtaacc ttctccccct aatctgtata gtataatata aagggcatag gagtgttggg
     4321 gatgaaaaga tatacgcata accataaaca taagcataaa cacgatagta gcaaaattgt
     4381 gggggtatcc tta
//
LOCUS       DATGSI010000340         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_213861, whole genome shotgun sequence.
ACCESSION   DATGSI010000340 DATGSI010000000
VERSION     DATGSI010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213861"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..318
                     /locus_tag="VK031_05285"
     CDS             109..318
                     /locus_tag="VK031_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HLS53364.1"
                     /translation="MSKKKRRKKKKIKTADDLLKYEIAAELGLLDKIEEVGWGGLTAK
                     ESGRIGGIMTAKKRKKQAENKKEVD"
     gene            324..1058
                     /locus_tag="VK031_05290"
     CDS             324..1058
                     /locus_tag="VK031_05290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLS53365.1"
                     /translation="MYKEFANLYDQLMNDFDYKEWFLYIEEIFTKFKVSPEKVLEMAC
                     GTGNLTYYLGEKGYKLTAFDLSEEMLAIAYKKLNKFKNIKLLKQDMTSFQLNEKYQAV
                     VSICDSINYILCENQLLNTFKNVYKHLETDGIFIFDINSEYKLQEIIGENIFVEDREN
                     IFYIWDNDFEQDRKIHNFYLTFFYGADGINFRRTDEYHRQRSYKEEEVKDLLTSAGFN
                     HVYSYEAFSFKPVAANSERINFVALK"
     gene            1182..1382
                     /locus_tag="VK031_05295"
     CDS             1182..1382
                     /locus_tag="VK031_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLS53366.1"
                     /translation="MEKGTVKWFNATKGFGFISTDEGNDVFVHYSAIQGEGFKTLDEG
                     DQVEFELVEGEKGPQATNVVKL"
     gene            1471..2349
                     /gene="hslO"
                     /locus_tag="VK031_05300"
     CDS             1471..2349
                     /gene="hslO"
                     /locus_tag="VK031_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HLS53367.1"
                     /translation="MTDYFIRGIDKSGRIRILVGTTTKMVEEARKIHQTSPTATAALG
                     RALTAGALMGAMMKNQQDRLTLRIAGDGPAGNILVVARNDGKVKGEMTNPQADVPSRP
                     DGKLDVGGLVGRNGTITTIMDLGLREPYIGQSQLVSGEIAEDLANYYYYSEQKPSAVS
                     LGVLVDKDISVKAAGGYIIQLLPGISDQDIDKIERSLAKIQPVSSLVDNGLSPEEIMT
                     ELLQDFSMEVLERRDLSYECDCSRERIERGIISLGKEEIKSIIEEEGQAEVVCHFCNT
                     KYQFSKEDLEKFLIDI"
     gene            2384..2459
                     /locus_tag="VK031_05305"
     tRNA            2384..2459
                     /locus_tag="VK031_05305"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2417..2419,aa:Phe,seq:gaa)
     gene            2468..2542
                     /locus_tag="VK031_05310"
     tRNA            2468..2542
                     /locus_tag="VK031_05310"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2500..2502,aa:Gly,seq:gcc)
     gene            2574..2648
                     /locus_tag="VK031_05315"
     tRNA            2574..2648
                     /locus_tag="VK031_05315"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2606..2608,aa:Cys,seq:gca)
BASE COUNT          972 a          384 c          612 g          742 t
ORIGIN      
        1 accagtttta gtggtgatta tagtatttat tttctttaac tggaaattag ttatagttta
       61 gaataaatat taagtcttat tcccataata taagatggag gggataagat gagtaagaaa
      121 aaaagaagga aaaagaaaaa gataaaaacg gcagatgatt tattaaaata tgaaatagca
      181 gctgaactgg gcctgttaga taaaatagaa gaagtaggct ggggtggttt gacagccaag
      241 gaatcaggca ggataggagg cataatgact gccaaaaaga gaaagaagca ggctgaaaac
      301 aagaaagagg tagactaagt tttatgtaca aggaatttgc aaatctttat gaccagttga
      361 tgaatgattt tgattataag gaatggtttt tatatataga agaaatattt actaaattca
      421 aggtaagccc tgaaaaagtg ctagaaatgg cctgtgggac cggtaattta acctattatt
      481 taggcgaaaa ggggtacaag cttacagcct ttgacctatc agaggaaatg ctggctatag
      541 cctacaaaaa attaaacaaa tttaaaaata ttaaactctt aaaacaagat atgacaagct
      601 ttcagttaaa tgaaaaatac caggccgtag tatcaatctg tgatagcata aattatatac
      661 tatgtgaaaa ccaattatta aataccttta aaaacgtata caaacatttg gaaacagatg
      721 gaatttttat ctttgatata aacagtgaat ataagttaca agaaattata ggagaaaata
      781 tttttgttga agacagggaa aatatttttt atatctggga caatgatttt gaacaggata
      841 gaaaaataca caacttttat ctaacctttt tttatggggc agatggaatt aattttagaa
      901 ggacagacga atatcatagg cagagatctt ataaagaaga ggaagttaaa gatttgttaa
      961 ccagtgcagg ttttaaccat gtttactcct atgaagcctt cagctttaaa ccagtagcag
     1021 caaatagtga aagaataaat tttgtagctt tgaaataatt ctaggtggat aagtaggatt
     1081 attttagggt atattattga catatagaaa tatactgtta tagtataatc taataggtaa
     1141 aatacttatt ttaccaaaag ctacattgga ggaatgttta aatggaaaaa ggtactgtaa
     1201 aatggttcaa cgcaacaaaa ggatttggat tcatctcaac agatgagggt aatgacgtat
     1261 ttgttcatta ttcagcaatc caaggagaag gcttcaaaac tttagatgaa ggagatcaag
     1321 tagaatttga attagttgag ggtgaaaaag gtccacaggc aactaatgtg gttaaactat
     1381 aaattctaga tcttaatata tagtttagaa gtttttagaa gttgaaagag ccccgagagg
     1441 ggctcttagt attgaaatga ggtgaaagtg atgacagatt atttcataag aggaatagat
     1501 aagtcaggca gaattaggat actggttgga actacaacta aaatggtaga agaggctaga
     1561 aagatccatc aaacatctcc aactgccact gcagcattag gccgggccct gactgctgga
     1621 gccttaatgg gggctatgat gaaaaatcag caggataggc taaccttaag aatagcaggg
     1681 gatgggcctg ctggaaatat tttagtagtg gctagaaatg atggtaaggt aaaaggagaa
     1741 atgaccaatc ctcaagcaga tgtaccaagt aggccagatg gcaagttgga tgtaggtgga
     1801 ctggtaggta ggaatggcac aataaccacc attatggatt taggtttaag ggagccctat
     1861 ataggtcaat ctcaattagt aagtggagaa attgcagagg acttggccaa ttactattac
     1921 tattctgaac aaaaaccttc tgcagtcagc ttgggagtcc tggtagataa agacatatca
     1981 gttaaggcag caggcggtta tataatccag ttgctacctg gcatatcaga ccaagatata
     2041 gataaaatag aaaggtccct tgccaaaata cagccagttt ccagcctggt tgataatggt
     2101 cttagtccgg aagaaattat gacagaactg cttcaagact tttccatgga ggtcttagaa
     2161 agaagggatt tgtcctatga gtgtgattgc tccagggaaa ggatagagag gggaatcata
     2221 agtttaggta aggaagaaat taaatcaatc atagaagaag aaggccaagc agaagtagtc
     2281 tgccattttt gtaataccaa gtatcaattt agcaaggaag atttagaaaa atttcttatt
     2341 gacatttaat atattttaat gtatactatc ttatgtggtg ctggcccaga tagctcagtc
     2401 ggtagagcag tagactgaaa atctacgtgt cgctggttcg attccggctc tgggcaccag
     2461 ggtataggcg gaaatggctc agtggtagag catcgccttg ccaaggcgag ggtcgcgggt
     2521 tcgaatcccg ttttccgctc caataatact accatggaac agcactaaaa tttggcggca
     2581 tagccaagcg gtaaggcaga ggtctgcaaa acctttattc cccggttcaa atccgggtgc
     2641 cgcctccaaa attcacccct tataggggat ttttagtata atacctactg ctggatgtaa
     2701 acaggcagta
//
LOCUS       DATGSI010000341         1461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_684801, whole genome shotgun sequence.
ACCESSION   DATGSI010000341 DATGSI010000000
VERSION     DATGSI010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684801"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VK031_05320"
     CDS             complement(<1..935)
                     /locus_tag="VK031_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HLS53368.1"
                     /translation="MESDNSSSSKMIGILIAFFLVAALSLGFYVYQGKPVSLSVDGTT
                     TKITSRSKTVEELIEEEEVKFEKGAYINVPLDTELEKDLNIMIINPKPYTVKVRDTYL
                     EVRSIYDTADQILKEQGLKLGQLDYTEPGLKERLSPGSTIEVFRVNEELVVEESTIPF
                     DEEVKTTDKLDRGTKRIAQKGQEGLKRTYIRKKYINGQLVSTAVEKEEIAKEPVTQIV
                     EKGTRERVVPTSRGNTRYRKAITMQATGYDLSYQSTGKSPGDKYYGITASGMRARVGV
                     VAIDPRVIPYGTKLYIKSLDGTKDYGFAIAGDTGGA"
     misc_binding    1164..1395
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          404 a          289 c          251 g          517 t
ORIGIN      
        1 gcaccgccag tatctccagc aatggcaaat ccatagtcct tggtcccatc tagggactta
       61 atgtacagtt ttgtaccata gggtatcacc cttggatcta tggctacaac acctacccta
      121 gccctcatac cagaggctgt aatgccgtag tacttgtctc ctggactttt accagtgctt
      181 tggtaagata agtcatatcc tgttgcctgc atggtaatag cctttctgta tctggtgtta
      241 ccccttgatg ttggaactac tctttctcta gtaccctttt ccactatctg ggttactggt
      301 tctttagcaa tttcttcctt ttcaacagct gtgctaacca gttgaccatt aatgtacttt
      361 ttcctaatat aggttctttt taaaccttct tgaccttttt gggctatcct tttggttcct
      421 ctatctaact tatctgttgt tttcacttct tcatcaaagg ggatggtaga ttcttctact
      481 actagttctt cattaaccct aaatacttct attgtacttc cagggctaag tctttctttt
      541 aagccaggtt ctgtatagtc tagttgacct agcttcaacc cctgttcctt taagatttgg
      601 tctgctgtat catagataga ccgaacctcc aagtaggtgt cccttacctt gacagtgtaa
      661 ggtttaggat ttataatcat tatgtttaag tctttttcta attcagtgtc taaaggaaca
      721 ttaatatagg ctcctttttc aaatttaact tcctcctcct caataagttc ctcaacagtt
      781 ttggaacgag aagttatttt ggtagttgta ccatctacac taagacttac tggtttacct
      841 tggtaaacat agaatcctag agataaagct gccactagaa agaaggcaat caggattcct
      901 atcatcttag agctagatga attatcactt tccattttaa acctcctaat ttgtaacttt
      961 tttgtaacta attatgttac tgttgtattt taatccacat tccatatttt gtcaaacctt
     1021 attttaatat ttatattatt ccttaataat tattaattac caacttactg aaatttattc
     1081 catttggctg aaaatcctat ttgttgacaa aatcacctgg tttacgtata ataattataa
     1141 aattaaccag aatatactaa aagcaaaaga tgaagatggg aagagtagga ttaggtttat
     1201 tctacaggaa gctgggctag gctgaaagcc agtgtttaaa tttaatctga agatggcacc
     1261 tgaatcccac agttgaaggc aagtagactg tggcggagac agccgttacc tgtcaggtta
     1321 taaaccggca aaggtctaaa ttgcgaaatt tagataaatt agagtggtac cacgaactat
     1381 accttcgtct ctagggagat gggggatttt tttatgatta ggtaagattt aattctatcc
     1441 caaggaggtt aacagatgaa a
//
LOCUS       DATGSI010000342         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_255569, whole genome shotgun sequence.
ACCESSION   DATGSI010000342 DATGSI010000000
VERSION     DATGSI010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255569"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VK031_05325"
     CDS             <1..381
                     /locus_tag="VK031_05325"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HLS53369.1"
                     /translation="SELRCTEYIPNIVMKQFGRDCFIYSEPTKEDIKKAREGLQKIQL
                     ILREGKYDLVVLDEINIALYYKLFTVDEVVKAIKDRAYHVEVAITGRYAPDKLIDLAD
                     LVTEMKEVKHYYTKGVEARTGIDK"
     gene            536..1879
                     /locus_tag="VK031_05330"
     CDS             536..1879
                     /locus_tag="VK031_05330"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HLS53370.1"
                     /translation="MLVKEKREETGGRNKGQLIIDGHNDTMMKIVDEKTWLPKVDIGE
                     DTDFHIDIPKLEKGGVNVPCFASYTRGYGHNFDKSNSRTLALINALYWTEERNYHNFQ
                     IVRSINELEQIVEKGKIAGILTIEGAYGLNRQNGIELIRQYYDLGAKMLAPTWNYSNE
                     LGEGAHQVYGDSLKAPSPKGLTDFGKEIITEMNRLGMIVDVSHLSEGSFWDVIETSKA
                     PIVASHSGVASLKKHPRNLTDKQLLAIKENGGVVGLVLCAGFIADKEEVYITDYVDHI
                     DYAVNLMGVDHVGIGSDFDGAKMPKDLEDASKFQKIVDELKARGYSKEDMDKILGLNF
                     LRVFKEAEEKREVDKLEEISILITCQMGELINKKDQLPKARLMLDDGVKIDLNKSRAI
                     IDGMVYPVLEEENTGYLYVDFKESLIEGFHTITYEVYEEGGSFGRETKIFYIDNN"
     gene            2065..>3008
                     /locus_tag="VK031_05335"
     CDS             2065..>3008
                     /locus_tag="VK031_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLS53371.1"
                     /translation="MMKTYEADKIRNVALIGHSGSGKTTLTEAILYVTGVTKRQGKVD
                     DGNTVSDFGKEEIARKVSIGTASIPVEWNDRKFNILDTPGYFDFVGETYGALRASEGA
                     ILMVNATAGIEVGTEKAWNLLEKHKRPRFIFINKMDQENVNFDKLIDELKENFGNKIF
                     PFALPIGAGEKFKGFVNVRDEVAYEYKDGQRKEIPLFDEEKTAIEDIRESMMEIVAES
                     DDELLEKYFEGEEFTTEEIAWGIRTAVANGDLVPVFIGSAEKAIGIDYLLSVIDRYIP
                     SAKDVGSVKAYKGEEEIEVKIDENEPFSAIVFKTIVDP"
BASE COUNT         1117 a          382 c          705 g          804 t
ORIGIN      
        1 agtgaattaa ggtgtactga atatatacct aatattgtaa tgaagcagtt tggtagagat
       61 tgctttatct atagtgagcc aacaaaagaa gatattaaaa aggccagaga aggtctgcaa
      121 aagattcaac ttatcttaag ggagggtaaa tatgatctgg tggtattgga tgaaataaat
      181 attgcccttt attataagct ctttactgtg gatgaggtgg ttaaggccat aaaagatagg
      241 gcttatcatg tggaagtggc catcacaggg agatatgcac cagataaatt aatagacttg
      301 gctgacctgg taacagaaat gaaggaagta aaacattact ataccaaagg ggttgaagcc
      361 aggacaggta tagataaata ggtttagaaa aaactaatca ttttccacta acatctttac
      421 ataggacaat aaaggggtgg agttttatgt gaggattaaa aggaagttaa cctttcttgt
      481 tatactaata ttcttaatgg ttttgtataa atttacttca aagggggata taaatatgct
      541 ggttaaagaa aagagagaag aaacaggggg aaggaataag ggacagttaa taatagatgg
      601 acacaatgat acaatgatga aaattgtaga tgaaaaaacc tggctaccta aggtggacat
      661 aggtgaagat actgattttc atatagatat tccgaagcta gaaaaaggtg gcgtaaatgt
      721 tccttgcttt gcctcctata cccgcggcta cggtcataac tttgataaaa gtaatagtag
      781 aaccttggcc ctaataaatg ccctttactg gacagaggaa agaaattacc acaatttcca
      841 aatagtaagg tcaataaatg aattggaaca aatagtagaa aaaggaaaaa tagctggaat
      901 attgacaata gaaggagctt atggactaaa ccggcaaaat ggaatagagc taataagaca
      961 atattacgat ttaggagcta aaatgttagc gcctacttgg aactattcca atgaattagg
     1021 ggaaggggcg caccaggtat atggagattc attaaaggca ccatctccca aagggttaac
     1081 agattttgga aaagagataa taactgaaat gaacaggcta ggtatgatag tggatgtatc
     1141 ccatctgtca gaaggctcct tttgggatgt aatagaaaca agcaaggcgc caatagttgc
     1201 ttcccattct ggagtggctt cactaaagaa gcatcctaga aacctgacag acaagcaact
     1261 tctagccatt aaggaaaatg gtggtgttgt aggattagtt ctttgtgcag gatttattgc
     1321 agacaaagaa gaggtttaca taacagatta tgtagaccat atagattatg cagtaaactt
     1381 gatgggagta gaccatgtag gtataggttc agattttgat ggagccaaaa tgcctaagga
     1441 tttggaagat gcttcaaagt ttcaaaagat tgtagatgaa ttaaaagcaa ggggttatag
     1501 taaggaagat atggataaaa tccttggatt aaacttctta agagtattta aggaggctga
     1561 agagaaaagg gaagtagata agttagaaga aatttcaata ttgatcacct gccaaatggg
     1621 ggagctaatt aataaaaaag atcagctacc taaggccagg ctaatgctgg atgatggggt
     1681 aaaaatagat cttaataagt ccagagctat tatagatggt atggtatatc ctgtattaga
     1741 ggaagaaaat acaggctatc tatatgtaga ctttaaagaa agtttgatag aaggcttcca
     1801 tacaattaca tatgaggtct atgaagaggg gggaagtttt ggtagggaaa caaaaatatt
     1861 ttatatagat aataattaaa aactgaactt tcctattgga ctgtaaaaca caaagccagg
     1921 taaaaaagac taggataaaa taggatttca tgatataatc tataccatat aggattttca
     1981 ggatacccat tatacttagt ttgatgaaaa ggttttacta ggataaaatc ttttaaataa
     2041 atacactggt taagggggca atatatgatg aaaacatatg aagcggacaa aattaggaat
     2101 gtagctttaa tagggcacag tggtagtgga aagaccacac tgacagaggc aattttatat
     2161 gtaacaggag ttaccaagag acagggaaaa gtagatgatg gaaacacagt ttccgacttt
     2221 ggaaaggaag agattgccag gaaggtttcc ataggaactg catcaatacc agttgaatgg
     2281 aatgatagga agtttaacat attagatact cctggatatt ttgactttgt tggtgagaca
     2341 tatggagccc taagagcaag tgaaggtgcc atattaatgg ttaatgcaac tgctggaatt
     2401 gaagttggga cagaaaaggc atggaacttg ctggaaaaac ataaaagacc aagatttatt
     2461 ttcatcaata aaatggacca ggaaaatgta aactttgata aattaataga tgaactgaaa
     2521 gaaaactttg gaaacaaaat ctttcccttt gcgctaccaa ttggagccgg agaaaagttt
     2581 aagggttttg taaatgtaag agatgaagta gcctatgagt ataaggacgg acaaagaaaa
     2641 gaaataccct tatttgatga ggaaaagaca gccatagagg atattagaga gagtatgatg
     2701 gaaatagtag cagaatcaga tgatgagctg ttggaaaagt attttgaggg tgaggaattt
     2761 actactgagg aaatagcctg gggtattagg actgctgtgg ccaatggaga tttggtacct
     2821 gtatttattg gttctgcaga aaaggctatc ggtattgact atcttttaag tgtaatagat
     2881 aggtatattc catcagcaaa ggatgttggt tctgtcaagg cctataaggg agaagaagag
     2941 atagaagtta agatagatga aaatgaaccc ttctctgcca tagtctttaa gaccatagtg
     3001 gacccctt
//
LOCUS       DATGSI010000343         3936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_495697, whole genome shotgun sequence.
ACCESSION   DATGSI010000343 DATGSI010000000
VERSION     DATGSI010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495697"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1039
                     /locus_tag="VK031_05340"
     CDS             287..1039
                     /locus_tag="VK031_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53372.1"
                     /translation="MIYRFENLESRMQEVANNLNFIKLTDDEKHYYNIRTGKKYQRVT
                     SYISDEKVEMTPLLLSSVTIGTKVDNLVRDYFDGNLKSPDTYQLSTIDQINHFTNQLK
                     ELKDVFDARGETVLPNDIVLYNDEIGVAGTVDLISYDVNGVIRIYDMKTMRGNQFTTT
                     YYGDNQYKYYSTMYGMSNQEKHQRQISLYNILLNNTHGLRASAAGIIPIVVKYNPEDT
                     TTKKLHMLPRLRHKLLDKVNDAEIDQGVGTSF"
     gene            1093..1356
                     /locus_tag="VK031_05345"
     CDS             1093..1356
                     /locus_tag="VK031_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53373.1"
                     /translation="MTERNFATNFYDTANDIISLCIRDAIWQMNDYSQEEVTIEREEV
                     DDTGGILYYEDEPLLVFDTDFKNTNWMIYVKEKYHRRSKDSNE"
     gene            1349..1573
                     /locus_tag="VK031_05350"
     CDS             1349..1573
                     /locus_tag="VK031_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53374.1"
                     /translation="MSDFLVLLEAEEEAKKSVDRSKEAIDMLGHFLQFLKTKDYFKDI
                     VEEFNASHNRLSIGVKDNRVFIYLDGEKIK"
     gene            1570..1908
                     /locus_tag="VK031_05355"
     CDS             1570..1908
                     /locus_tag="VK031_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53375.1"
                     /translation="MTRRYKIKITKTREFVSLFNGIFKLSPKEIEIISEFIDMAAKLR
                     GTGINPFSTEIKKKVSDNLGRDDFNTLNNYIKSLADKRAISKTDNGYNIHPMLLPEQV
                     DKIEFHLIHE"
     gene            1901..2128
                     /locus_tag="VK031_05360"
     CDS             1901..2128
                     /locus_tag="VK031_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HLS53376.1"
                     /translation="MSKPLYQTGIIKQLANKYNLPEEEVAKAVYHQFKFVSKTMKRGL
                     FESVRLRRFGRFHVNPNRVKKLNARRSNTNE"
     gene            2121..2525
                     /locus_tag="VK031_05365"
     CDS             2121..2525
                     /locus_tag="VK031_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53377.1"
                     /translation="MNKVRERKEKDEAKRQFIEMMEKYNQDADANFDGIELRGRHFII
                     KAPKIETESGLILTEETRKTIQAVTDSPTFKEEFKVELASKQCDFIEAGQLVLLSPHV
                     QTLEIALPTPEGEEVLYLMIHEDYVLATKEVD"
     gene            2525..2758
                     /locus_tag="VK031_05370"
     CDS             2525..2758
                     /locus_tag="VK031_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53378.1"
                     /translation="MRVYTVERLSELIVHELFKNVKVGFLLPFLSFSVYEETEEVDQG
                     ALSVRRSYYLTIGKKKYNWEVSIYDTRKEVIDG"
     gene            2751..3008
                     /locus_tag="VK031_05375"
     CDS             2751..3008
                     /locus_tag="VK031_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53379.1"
                     /translation="MDESKLTIEEEIVANKELRVELDNVLQKLKSLPLSRDRQISAIN
                     LKQAIMWLGMDLKRLNEPNPYPESYNPNNSKVSPTADNLKL"
     gene            3005..3544
                     /locus_tag="VK031_05380"
     CDS             3005..3544
                     /locus_tag="VK031_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53380.1"
                     /translation="MKLLDVNNMVGIPSPEASQFEPYLKIIKRDRTRGRAKAAKELAF
                     IYYMWHWESPYAAYEESLREEKIIEDIFPDEDWTPDDRVLDACRLYREQTENEYIRLL
                     QAARTAANKLIDYFETTDLNERDERDRPIHTAKDMINNISKVGEVVEGIDKLKEIIEK
                     NQSKNSSNRAGVETNKYSE"
     gene            3698..>3936
                     /locus_tag="VK031_05385"
     CDS             3698..>3936
                     /locus_tag="VK031_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53381.1"
                     /translation="MTMDTDLTLDTLYSSIKMDYSFPPEVVWFIEGPLKDTKRKFPEE
                     VTTYTYEDKTLPKESTFMIVDGEEIEVLDNKILYKL"
BASE COUNT         1483 a          623 c          779 g         1051 t
ORIGIN      
        1 tatccactcc aatatatagg gggggtcaat ctaacactga ataactgccc ccacctatcc
       61 tattaggaca agtagccttg aatacacaca ccccatctat tatattgaca gctaaatcat
      121 atttatatga agaaacaata ttactggaag agtgaataca acgaatacaa atattggata
      181 agagacagta gatataacag agactacaga aggttttgta aagggcagta tcacaaggca
      241 agaagaagac acgacaaaca aataattgaa gaggagctag aggacattga tctataggtt
      301 tgagaacttg gaatccagaa tgcaagaggt agctaataac ctcaatttca ttaaactcac
      361 tgatgatgaa aagcattatt acaacataag aacaggaaag aagtatcaga gggtaactag
      421 ttatatatca gatgagaagg tagagatgac acctttgctt ttatcttctg tcactattgg
      481 aactaaagtg gataatctgg ttagagacta ctttgatggt aatctcaaat cacccgatac
      541 ttatcaatta tcgactattg accagataaa tcacttcact aatcaactca aagagttaaa
      601 ggatgtattt gatgctagag gtgaaacagt tctacctaat gatattgtac tctataatga
      661 tgagataggt gttgcaggta cagtggatct catatcctac gatgtcaacg gagttattag
      721 gatctatgac atgaaaacta tgagaggtaa tcaattcact actacctact acggggataa
      781 tcaatacaag tattattcaa ctatgtatgg aatgtctaat caagagaaac accaaagaca
      841 gatctcattg tacaatatct tattaaataa tactcatggt cttagggctt cagctgctgg
      901 aatcatacct attgttgtta aatacaatcc agaggataca actactaaga agctccatat
      961 gctccccaga ttaagacaca agcttcttga taaagtaaat gatgcagaaa ttgatcaagg
     1021 agttggtact agcttctaaa atcactatta ttgcgttata acaagaatct ttattatatt
     1081 atcaagcaga gtatgacaga aagaaacttc gctaccaact tctatgatac agccaatgat
     1141 attatatctc tatgtatcag agatgctatt tggcagatga atgactatag tcaagaagaa
     1201 gtcactatag aaagggaaga ggtagatgac acaggaggta tcctttatta tgaagatgaa
     1261 cctcttctag tatttgatac cgactttaaa aacactaatt ggatgatata tgtcaaagaa
     1321 aaatatcaca ggagaagcaa ggattccaat gagtgatttt cttgtcctgt tagaagcaga
     1381 ggaggaggct aagaaatcgg tagatagaag taaagaagct attgacatgc ttggtcactt
     1441 tctacagttt ctaaagacaa aagattactt taaggatata gtagaggagt tcaatgcttc
     1501 acataacagg ctttcaatag gagtcaaaga caatagagta ttcatctatt tagatggaga
     1561 gaagatcaaa tgactaggag atataagata aagatcacaa agactagaga gtttgttagt
     1621 ctatttaatg gaatattcaa gctctctcct aaagagatag agatcatctc agagtttata
     1681 gatatggcag ctaagctaag aggaacaggt atcaacccat tctccacaga gattaaaaag
     1741 aaagtatctg acaacttagg cagagacgat ttcaatactc taaacaacta tataaagtct
     1801 ctagcagata aaagagctat ctctaaaaca gacaatggct acaacatcca tcctatgctt
     1861 ctaccagaac aagtggataa aatagagttt cacctgatac atgagtaagc cactatatca
     1921 gacaggtatt ataaagcagc tagctaacaa atataatcta cctgaagaag aggtagctaa
     1981 ggctgtatat caccagttca agtttgtctc taagactatg aagagaggct tatttgaatc
     2041 tgttagatta agaagatttg gaagatttca tgttaaccca aaccgagtta aaaagttaaa
     2101 tgcaagacga tcaaacacta atgaataagg tacgagaacg taaagagaaa gatgaagcca
     2161 agaggcaatt catcgaaatg atggagaaat acaatcaaga tgctgatgct aactttgatg
     2221 gtattgagct cagaggacgt cattttatta ttaaagctcc taagattgag actgaatcag
     2281 gtcttatcct aactgaggaa actagaaaga ctattcaagc tgtaacggat agtccaacat
     2341 tcaaagaaga attcaaagta gagttagctt ctaaacagtg tgattttatt gaggccgggc
     2401 aactagtact tctgtctcct catgtacaga cattagagat tgctcttcca acaccagaag
     2461 gagaagaagt tctctatcta atgattcatg aagactatgt actcgcaact aaagaggtag
     2521 attaatgaga gtatatactg tagaaaggct atctgaactt attgttcatg agctctttaa
     2581 gaatgtaaaa gtaggattcc tacttccatt cttatcattc agtgtctatg aagagactga
     2641 agaggtagat caaggagctt tgagtgtaag aaggtcctac tatctgacta ttgggaaaaa
     2701 gaagtataat tgggaagtaa gcatctatga taccagaaag gaggtgattg atggatgaga
     2761 gtaagttaac aatagaagaa gagatagtag ccaataagga gcttagagtt gaattagata
     2821 atgtgctcca gaagctaaag agtctcccac ttagtaggga caggcagata tctgctatta
     2881 atcttaaaca agctattatg tggttaggga tggatcttaa aagacttaat gagccaaatc
     2941 cttatccaga aagctataat ccaaacaata gtaaagtttc accaacagct gataacctaa
     3001 aactttgaag ctattagatg taaataacat ggtgggtata ccctcaccag aagctagtca
     3061 gtttgagcct tatctcaaga ttatcaaaag agatagaacc agaggtaggg ctaaagctgc
     3121 taaggaacta gcctttatct attatatgtg gcattgggaa tctccctatg ctgcatatga
     3181 ggagtcgctt agagaggaga agattataga ggatatattc ccagatgaag attggactcc
     3241 agatgataga gtattagatg cttgcagatt atatagagag caaacagaga atgaatatat
     3301 aagactactc caagcagcca gaactgctgc taataaactc attgattact ttgaaaccac
     3361 tgatctcaat gagagagatg agagagatag acctattcat actgccaaag acatgataaa
     3421 caacatctca aaggtaggtg aagtagttga aggaatagat aaactcaaag agatcataga
     3481 aaagaatcaa tcaaagaata gttctaatag agcaggagta gagacaaata aatactcaga
     3541 atgatagaag agcttaagat agcattaagt gaacaatttg aagataataa caatgagaga
     3601 tcattctctc ttaatagaga caactatgaa aggtgtctct tttttctagt agatcaaggt
     3661 tggaaccgtg actacatcat atcagcaaca acagtagatg acaatggata cagacttgac
     3721 attagatacc ttgtattcct cgataaaaat ggattattct ttcccacctg aagtggtatg
     3781 gtttatagaa ggtccactaa aagatactaa acgaaagttt ccagaagaag taactaccta
     3841 cacctatgag gataaaacac tacctaaaga gtctactttt atgatagtag atggagaaga
     3901 gatagaagta ctggataata aaatactcta caagct
//
LOCUS       DATGSI010000344         1914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_213975, whole genome shotgun sequence.
ACCESSION   DATGSI010000344 DATGSI010000000
VERSION     DATGSI010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213975"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="VK031_05390"
     CDS             complement(<1..1426)
                     /locus_tag="VK031_05390"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLS53382.1"
                     /translation="MFKRLKKYLYIFFIFIILVACSQQNRDISTLINKDLNNIDPNKL
                     TQYEIEVDLDDENMLYTGKQWVDYVNRTGKVLGEIYFHLYPNAFKNFDQAPILFSKDK
                     YSKSNYVGGYIDIEKILINNKPVHFSILEDDETTLYIKLEEPLLQGQSQRIYLEYKVK
                     LPSSRDRFGYGHRTINFGNWYPIVCVYDENGWNIDPYYQVGDPFYSDLANYKVTIKTH
                     KDMVVASSGNIVSSKLEADRKVYNIEGRMIRDFAWVASKEFKVEDRKIDETLVKLYYL
                     DRNYDMVNKSLQVGGDSLQIFNRIFGQYPYGVYSIVMTEFPSGMEYPGIVFIGEEYFR
                     NSLTYILEQIIVHETAHQWWYGLVGNNQIREAWLDEGLTTFSEVIYNREKYGKKQGED
                     YFVQNIKLGYELGQSYLIGEENIVNKPLNEFNDWNDYSLLVYSKGAMFINAIEEEFGQ
                     KALYKILNNYYERYKYHNATLDDFI"
BASE COUNT          635 a          352 c          240 g          687 t
ORIGIN      
        1 ttatgaaatc atctaaagtt gcattatggt atttatatct ttcataataa ttatttaaaa
       61 tcttgtataa ggctttctgc ccgaattctt cttcaatagc atttataaac atagctccct
      121 ttgaatatac caaaaggcta taatcgttcc aatcattaaa ttcattcagt ggcttattaa
      181 caatattttc ttcccctatt aaataagact gaccaagctc atatcctagc ttgatgtttt
      241 gaacaaaata gtcttcacct tgtttctttc catacttttc cctattataa attacttcag
      301 aaaaggttgt tagaccttca tccaaccaag cctccctaat ttggttatta cctactaaac
      361 cataccacca ttgatgggca gtttcatgga caatgatttg ctccaatatg taggttagag
      421 aatttctaaa atattcctcc cctataaaaa ctatgcctgg atattccata cctgaaggaa
      481 attctgtcat aacaatagaa taaacaccat aggggtattg gccaaaaatc ctattaaaaa
      541 tttgaagaga gtctccccct acttgtaaag acttatttac catatcatag tttctatcta
      601 aataatatag tttaaccaag gtttcatcta tcttcctgtc ttctacctta aattccttgc
      661 tggccaccca agcaaaatcc ctaatcattc ttccctctat attatatact ttcctgtcag
      721 cctctagctt acttgataca atgtttccac tgctggctac aaccatatcc ttatgagtct
      781 ttatagtaac cttgtaattg gctaaatctg aataaaaagg atcccctacc tggtaataag
      841 ggtctatatt ccacccattt tcatcatata cacaaactat gggataccag tttccaaaat
      901 tgatggtcct gtgcccataa ccaaatctat ccctgcttga agggagcttt accttatatt
      961 ccaaataaat cctttgactt tgcccttgta gtaaaggctc ctctagcttt atgtataagg
     1021 tggtttcatc atcttctaat atggaaaagt gtaccggttt attgttaatc aatatttttt
     1081 ctatatctat atatccacct acataatttg atttagaata cttatcttta gaaaacaaaa
     1141 taggagcctg gtcaaagttt ttaaaggcgt tgggataaag gtggaaatat atttcaccta
     1201 aaactttacc tgtcctattt acataatcaa cccattgctt tcctgtatat aacatatttt
     1261 catcatctaa gtccacctct atttcatatt gggtaagctt attaggatct atattattta
     1321 aatctttatt aattaaggta gaaatatccc tattttgttg gctacatgcc accaatatta
     1381 tgaatatgaa aaagatatat aggtattttt ttaatctttt aaacaattaa tcaccccatt
     1441 caaagccttg ccattattag ataatatatt ctttgattag gctcctttat tcatccatct
     1501 ttaaattagc atagagtttt atataatatt taaattaaat ctagcaactt gaaaggcaat
     1561 tatgaatata agaatagtga gatggaataa ttcttaatag atcttaatta aggattttct
     1621 aaaatcagta agggtaatat taaactaagt atattttaat aatttattta taatttattc
     1681 aaagaattta tacatgcaac atatatactt atatagacaa tataaataat gtataaatat
     1741 ttttatagat atgaaaaggg gaattattat gacaacagta gaaaatagca ggcgaagacg
     1801 aagaaagaag aaacaaccac ctacagcaac cttattggtt tactttgttc tatccatctt
     1861 ttttatggaa accatactaa ggcttagtac cacaggtgct cttttctctc ctgg
//
LOCUS       DATGSI010000345         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_353352, whole genome shotgun sequence.
ACCESSION   DATGSI010000345 DATGSI010000000
VERSION     DATGSI010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353352"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VK031_05395"
     CDS             <1..337
                     /locus_tag="VK031_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53383.1"
                     /translation="YKEENGKGRYENLVDAAKKELINIEKRLQLFAEAEAFLIKEAFI
                     IPYGVGGGGYIASRLNPFESPYSPFGVSAERFKGQKILKEPMDSKAYDIGLEKWEEKR
                     KEALKMSEW"
     gene            397..1341
                     /locus_tag="VK031_05400"
     CDS             397..1341
                     /locus_tag="VK031_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLS53384.1"
                     /translation="MIAYTFKRLIQSLITLIIVVTVVFLLMRMMPEEGYFGDGYEKLD
                     EKQREAILSNMGLRDPLHVQLSKFYKQILKGDLGRSIIYRPKVPISIILKDKVPYSLK
                     FGLAAMLISLLVGVPLGIIMALSKDSVLDRLGTVYIVLINAVPSAVYYLFLQIYITGL
                     FKLPILFSETNPISWILPTISMSLGGIASYAMWMRRYMVDELNKDYVKLARAKGMKSR
                     SIMIHHVLRNAFVPMAQYLPSAILFTIAGSIYIESLYSIPGMGGLLVDAIQRQDNTLV
                     QVLVLIYSSIGILGLFLGDIFMAILDPRIKLSKKEGAR"
     gene            1341..2309
                     /locus_tag="VK031_05405"
     CDS             1341..2309
                     /locus_tag="VK031_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLS53385.1"
                     /translation="MGNKKIEKLSDALNQSLFQFAQFKEYESRKAGYSNYSYWRSTFY
                     VFSKNRIAIVCLFLLVLLLTFTIIQPYLPNQKSPTQINIDPDTNMQLMNHPPSREFIF
                     GTNSIGQDLWARIWSGTRTSLMIGVLVGIWEAIIGIIVGAIWGYIRSLDPIITEIYNV
                     LNNIPTTIVLVLLTYIMQPSIGTLIFAMCMTGWLSMARFIRNQIVIIRDREYNLASKT
                     LGTSTMRIITKNLLPYLVSVIMLRLALSIPAAIGSEVFLTYIGLGLPVDIPSLGNLIN
                     EGRGVMMYKSLRYQLIFPAIVLSLITISFYVIGNVFADAADPRNHI"
     gene            2320..>3023
                     /locus_tag="VK031_05410"
     CDS             2320..>3023
                     /locus_tag="VK031_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS53386.1"
                     /translation="MKEKKAILSVNDLVIKFKLRGRVLTAIRGASLKLYQGETLAIVG
                     ESGSGKSVLTKSFMGLLDKNGWIDSGEIIYNGENLANYNRERDWLKIRGKEIAMVFQE
                     PMTSLNPLKTIGRQIQEAVELHQNIYGKAAKEEVLRILRDVDISDVTRRYNQYPHEFS
                     GGMRQRVVIAIAIACRPNILICDEPTTALDVTIQAQILQLIKELSKKYRLTTIYITHD
                     LGVVANVADRVAVMYAG"
BASE COUNT          974 a          487 c          623 g          939 t
ORIGIN      
        1 gtataaagaa gaaaatggaa agggaagata tgaaaatcta gttgatgctg ctaaaaaaga
       61 gttaatcaat atagaaaaaa ggcttcaatt gtttgcagaa gctgaggcat ttttgataaa
      121 agaagccttt attattcctt atggtgtggg aggtggaggt tatatagctt ccaggttaaa
      181 tccttttgaa tcaccttatt ctccctttgg agtgtctgca gaaagattta aagggcaaaa
      241 aatactgaag gaacctatgg atagcaaggc ttatgatata gggttagaaa aatgggagga
      301 aaaacggaag gaagcattaa aaatgtctga atggtaggaa attaatgtgt aagattaatc
      361 ttacacatta attttactta gcctaggggg gattaaatga tagcctatac ctttaaaagg
      421 ttaatacaat ctttaataac tttaataata gtggttactg tagtttttct tttgatgaga
      481 atgatgccag aagaaggata ctttggagac ggctatgaaa aattagatga gaaacagcgg
      541 gaggctatat tatccaatat gggcttaagg gaccccctac atgtccaatt atcaaaattt
      601 tataagcaaa ttctgaaagg cgacttaggc cgatccataa tatataggcc taaggtgcct
      661 atctctataa ttctgaaaga taaagttcca tactccttaa aatttgggct agctgccatg
      721 ttaatatctt tactggtagg agtcccttta ggaattataa tggccttaag taaggacagt
      781 gtgctggata gattagggac tgtatatata gttcttatta atgcagtgcc ttctgctgta
      841 tattatttat ttttacaaat atatataaca ggcctcttta aactacctat attattttca
      901 gaaactaatc ctatttcctg gatcctacct accatatcca tgtcattggg aggaatagcc
      961 tcctacgcca tgtggatgag aagatatatg gtagatgaac taaacaagga ttatgtaaaa
     1021 ttggccaggg ccaaaggaat gaaatcaaga agtattatga ttcaccatgt gttaaggaat
     1081 gcctttgttc caatggccca atatttacct agtgccattt tatttactat tgcaggatcc
     1141 atttatatag aatccttata ttctatacct gggatggggg gccttttagt agatgctata
     1201 caaaggcagg ataatacctt ggttcaggtt ttggtcttaa tctattcatc tataggaatt
     1261 ttaggcttat ttttgggaga cattttcatg gctattttag acccaaggat taagttaagc
     1321 aaaaaagaag gtgcaagata atgggtaata aaaagataga aaagctttca gacgccttaa
     1381 accaatctct ttttcaattt gcacagttta aagaatatga atcaagaaag gcaggctact
     1441 ccaactattc ttattggcgc tcaacctttt acgtttttag caaaaatagg attgccattg
     1501 tctgcctgtt tttactggtc ctactcttaa cttttaccat aattcaaccc tatttaccta
     1561 atcaaaaatc accaacccaa atcaatatag atcctgatac caatatgcaa ttgatgaacc
     1621 atccacctag cagagaattt atatttggca ccaattccat tggccaggat ctttgggctc
     1681 gtatttggag tggcacccgt acttctttaa tgattggtgt gctggtaggt atatgggagg
     1741 ccataatagg gattattgta ggtgccattt ggggttatat aagaagctta gatcctataa
     1801 ttactgaaat ttataatgta ttaaataata ttcctacaac tatagtactg gtccttttaa
     1861 catatataat gcaaccaagt attggtaccc taatttttgc catgtgcatg actggttggt
     1921 taagtatggc caggtttata agaaatcaaa tagtaataat cagagatagg gaatataatc
     1981 tggcttcaaa aactttgggg acttccacaa tgagaattat tacaaaaaac cttcttcctt
     2041 acttggtatc tgtaatcatg ttaaggctgg ccctatctat tccagcagcc attggttctg
     2101 aggttttctt aacctacata ggtctaggtc ttccagtaga tattccttcc ctaggcaatt
     2161 taattaatga gggtagggga gtaatgatgt ataaatcttt aagatatcag ttgatttttc
     2221 cagccatagt attatcctta ataaccatat ccttttatgt tattggtaat gtatttgctg
     2281 atgctgctga tccgcggaat catatctagg aggtagacta tgaaagaaaa gaaggcaatc
     2341 ctttctgtta atgatttagt tataaaattt aagcttaggg gtagggtgtt gacagctatt
     2401 aggggggcct ccttaaagct ttaccagggg gaaaccttgg ccatagtagg tgagtctggc
     2461 tccggcaaat ccgtattaac aaagtccttt atgggccttt tagataaaaa tggctggata
     2521 gactcaggtg agatcatcta taatggtgag aatttagcca attacaatag ggaaagggac
     2581 tggctaaaaa taagaggcaa agaaatagcc atggttttcc aagaacctat gacctcttta
     2641 aatcctttaa agactatagg aaggcaaatt caggaggcgg tggaactaca ccaaaatatt
     2701 tatgggaagg cagccaagga agaggtttta aggatcttaa gggatgttga tatttccgat
     2761 gtaactagaa gatacaatca ataccctcat gagttttctg gaggaatgag gcaaagagtg
     2821 gtaattgcca ttgccatagc ctgcaggcct aatattctaa tatgtgacga acctactaca
     2881 gccctagatg ttaccattca ggctcagata cttcagctga ttaaagagtt aagcaaaaaa
     2941 tacaggctga ccaccatata tataacccat gacttagggg tggtggccaa tgtggctgat
     3001 cgtgttgcag tcatgtatgc agg
//
LOCUS       DATGSI010000346         4165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_22032, whole genome shotgun sequence.
ACCESSION   DATGSI010000346 DATGSI010000000
VERSION     DATGSI010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22032"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1464)
                     /locus_tag="VK031_05415"
     CDS             complement(127..1464)
                     /locus_tag="VK031_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HLS53387.1"
                     /translation="MVKRRQLGVTIVGAGSTRTPALIGSLVNYKERFPLKKLILFDID
                     EDRMKVQEDYIRLTLEKYYPGVELIFTDNEDEAYIDVDYVFVQMRVGGFEMRSHDEKI
                     PLKYGLVGQETCGPGGFAYGMRSIKPMIHMVEKIREHNEDAWILNYTNPAAIVGLALN
                     KVFPQDDKILNMCDQPYSMIKSFAKILGVDMYDIEPRYFGLNHFGWFTKLYNAKTGED
                     LLPKLKKYLRSHEFRPYNAEQRDQSWLDTYKNVNRMMEYFPEYLPNTYLQYYFFADEI
                     AKASNPEFTRAEEAQAGREKQVLDICKKARKQQSLEGIPLLVGEVHGNMMVEVAESIA
                     YDLKKVFVVMGPNKERKIISNMPEDAFVEVAGELGKDGANLYPYGEIKTFYKGLMENQ
                     HAYEKLTVEACLESNYQKALEALTLNRTIVEPEKARLVLDDLMEANKDYWYLK"
     gene            complement(1467..2222)
                     /locus_tag="VK031_05420"
     CDS             complement(1467..2222)
                     /locus_tag="VK031_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HLS53388.1"
                     /translation="MKLSKLTQGYDELTDLEKKIVEYIMNNPEIVQDSPASKVADTLY
                     VSKTSIINLSKKLGFEGYSELRYYVKNYLEYKKKHKSALSYDKILSNIHTEITKTISL
                     QDEENINRIVRRIMDSRIVYVVGRGASGPIASLLSSRLALLNIKAILIADPNIIDVLG
                     NSLSQDESIIVMSLSGETERILTITKTARARGVDVIALTSFSNNTLQRIANYNMFCFA
                     DNTETKYNDLISRLGLHTLVQILISYIEMTDKE"
     gene            complement(2219..3796)
                     /locus_tag="VK031_05425"
     CDS             complement(2219..3796)
                     /locus_tag="VK031_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HLS53389.1"
                     /translation="MSRRKKFNENIQRFGRSLLLPIAVMAPVGMVLGISGALVQSYMI
                     ERLPFLGNPILNTILVSLRDIANIVFANIPLMFAMGVAYGMSRNQKGIAVFASVLSYL
                     VLLTTMNVWLKVSGTLADAEDMANVGQAMVLGIQTLRLDALGGIISGLIAAAVADRFY
                     DLELPLALAFFGGTRSVPIISMGLTMVVGIIIPFFWQFITNGLIKLSSILLNKPFGSF
                     IFITMNRLLIPFGLHHVWNSMIRFTAAGGTYTIAGETVVGIMPALNKIIFELGPSHES
                     WSLLPELTRFGSQNQMIMTMFALPAIGLAIYKTAFDENKKFVKGMVITMVLTAFLGNV
                     TEPVEFSFLFIAPLLFLFHSVAMGIVSVILFLMNTAVGYIRGTVFDFIIFGLMYENTR
                     WYNIILVGVPVFILYYIVFKWAIEKWNVPTPGRETDSFSHSRLLEERKYGEIAGIIVA
                     ALGGRENITHVDNCITRLRIDLLDNKKVDRDLLRESGTSGVFFPSSKHIHIVFGPHVE
                     FIKNALDRELKGELESN"
BASE COUNT         1274 a          883 c          580 g         1428 t
ORIGIN      
        1 ctatatattc ctcattaata gacttataaa taccttctat tcttccattt ttaactttca
       61 gatatccttt ttcataacct tgtggagtat agattctttc cgaatacaaa tacataatat
      121 cagctcctat tttaaatacc aataatcctt attggcctcc attaagtcat ccaatactaa
      181 cctggctttt tcaggctcca ctattgtcct gtttaaagta agtgcctcta aggccttctg
      241 atagttggat tccaaacaag cctccacagt tagtttttca taagcatgtt gattttccat
      301 caatccttta tagaatgttt taatctctcc atagggatat aaatttgccc catcctttcc
      361 taattctcca gccacctcta caaaggcatc ttctggcatg ttgcttataa tcttcctttc
      421 tttattaggg cccataacta caaatacctt ctttagatca tatgctattg attcagccac
      481 ctctaccatc atattcccat gaacttctcc aactagtaaa ggaatcccct ctagagactg
      541 ttgtttccta gccttcttac atatatccaa tacctgcttt tctctacctg cctgggcctc
      601 ttcagcccta gtaaattccg gattactagc cttggctatc tcgtcagcaa aaaagtaata
      661 ttgtagatag gtgttaggga gatattcagg aaagtattcc atcatcctgt taacattctt
      721 atatgtgtct agccaagatt gatctctttg ttccgcatta tatggtctaa attcatgact
      781 ccttaaatat ttctttagct taggcaaaag atcttcacca gttttagcat tatatagttt
      841 tgtaaaccaa ccaaaatggt ttagcccgaa atatctaggc tctatatcat acatatctac
      901 acctagtatt ttagcaaagg actttatcat agagtatggc tgatcacaca tattaagaat
      961 tttatcatct tgaggaaaaa ccttatttag ggctagtcca acaattgcag ctggattagt
     1021 ataatttagt atccaggcat cctcattgtg ttctcttatt ttttctacca tatgaatcat
     1081 aggctttatg gacctcatcc cataggcaaa tccaccaggt ccacatgttt cttggcccac
     1141 tagcccatac ttcaatggta ttttctcgtc atggcttctc atttcaaagc cgcctaccct
     1201 catttgaaca aatacatagt ctacatctat atatgcttca tcttcattgt ccgtaaatat
     1261 taactccaca cctggataat acttttccaa agttaacctt atatagtcct cttgtacctt
     1321 cattctatct tcatctatat caaacaggat caactttttt agtggaaatc tttccttata
     1381 attaactaaa ctaccaataa gggctggagt tctagtgctc cccgctccta ctatggtaac
     1441 ccctagttgt cttcttttca ccattatcac tccttgtctg tcatttcaat atatgatatc
     1501 aatatttgaa ctagagtgtg tagtccaagt cttgaaatca aatcattata tttagtttca
     1561 gtattatctg caaaacagaa catgttataa tttgcaattc tctgcaaggt attattggaa
     1621 aaagaagtaa gggcaatcac atcaactccc cttgcccttg cagttttggt tatagtcagt
     1681 attctctccg tctccccaga taatgacata acgataatac tttcatcttg agaaagtgag
     1741 ttacctaaca catcgattat attcggatca gcaatcaata ttgcctttat atttaagagg
     1801 gccagtctgg aactcaacag gctagctatt ggtccagagg ctcctctacc gactacatat
     1861 acaatcctag agtccatgat ccttctgaca atcctattta tgttttcctc atcttgcaga
     1921 gaaatagttt ttgtaatctc tgtatggata ttacttagta tcttgtcata ggatagggcc
     1981 gatttatgtt tctttttata ttctaaatag ttcttaacat agtacctaag ctcagagtat
     2041 ccttcaaatc ctaatttctt agataaatta ataatagatg ttttcgacac ataaagtgta
     2101 tctgctacct tgctggctgg agaatcctgt actatttctg ggttattcat aatatattca
     2161 actattttct tttcaagatc tgttaattca tcatatccct gtgtcaattt acttagtttc
     2221 aattagattc caactctcct tttagttctc tatctaatgc atttttaata aattccacat
     2281 gaggaccaaa cactatatgg atatgtttac tggatgggaa aaacacccct gatgttcctg
     2341 attcccttag aagatcccta tctaccttct tattatctaa taaatctatt ctcaatctag
     2401 taatacaatt atccacatgg gtaatgtttt cccttcctcc taaagctgca actataatcc
     2461 cagctatttc accatatttt ctctcttcta gaagcctact atggctgaag ctgtcagtct
     2521 ccctacctgg agttggtaca ttccattttt caatagccca tttaaagaca atgtaatata
     2581 gaataaaaac tggcacgcca actaaaataa tgttatacca ccttgtattc tcatacataa
     2641 ggccaaatat tataaaatcg aaaactgttc ccctaatata tcctactgcc gtattcatta
     2701 gaaatagtat tacactgaca atccccatgg ccacagagtg aaataggaat aacaagggtg
     2761 caataaacaa gaaagaaaat tctaccggtt ctgtaacatt tcctaaaaaa gccgttaaga
     2821 ccatggtaat taccatacct tttacaaatt tcttattttc atcaaaagca gttttataaa
     2881 tggccaaacc aatagctggt aatgcaaaca tggtcattat catttggttt tgagaaccga
     2941 acctagttaa ttcaggtaaa agactccacg attcatggct tggtcctagt tcgaatatta
     3001 ttttatttaa ggccggcata attcctacaa cggtttcccc agcaatagta taggttccac
     3061 ccgcagctgt aaaccttatc attgaattcc atacatggtg taatccaaat gggattaata
     3121 gcctattcat agttataaat ataaaggagc caaaaggttt atttaatagg attgatgata
     3181 atttaattaa tccattagta ataaactgcc agaaaaacgg gattataatt ccaactacca
     3241 ttgtaagtcc catggatata attggaactg atctggttcc accaaagaat gctaatgcca
     3301 aaggtaattc tagatcatag aacctatccg ccacggctgc tgcaataagc ccactaatta
     3361 taccacctag ggcatctaac cttagtgttt gaatacctag caccatggcc tgccctacat
     3421 ttgccatatc ttccgcatca gctaaagttc ctgatacttt taaccaaaca ttcatagtag
     3481 taagtaagac taggtaagac aaaactgatg caaatacagc aatacctttc tggtttctag
     3541 acatgccgta agctacaccc atagcaaaca tcaatggaat atttgcaaaa actatattgg
     3601 caatatctct caagctgact aagatagtgt taaggatagg attacctaaa aacggtaacc
     3661 tttcgatcat atagctttgc accaatgccc cgctaattcc aagtaccata cctacaggcg
     3721 ccatcacagc tataggtaat aataaggaac gaccaaacct ctgaatattc tcattaaact
     3781 tcttacgtct tgacactttc ttgctccccc tttgtttgaa aatttagaat ttattatata
     3841 aaatttattt actatagaat actatatgtt tttttatttc acaagatatt accggtattt
     3901 aggtctttga ccgggatatt ttactttggg cattttttat gactttggtc agttaatatg
     3961 taaaaaatgc ctcttcactc taaattttac catagagata aagagatata taattgttta
     4021 ctagtctcgc ttttgatttt taaagcctgg acctcctact aagtttttta gcttccttta
     4081 aataaaatct ggtattttgt cttccttgaa attgaggcat aaaagaccga aaactatatt
     4141 taaaatatgt attagattaa aataa
//
LOCUS       DATGSI010000347         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_496248, whole genome shotgun sequence.
ACCESSION   DATGSI010000347 DATGSI010000000
VERSION     DATGSI010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496248"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VK031_05430"
     CDS             complement(<1..329)
                     /locus_tag="VK031_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53390.1"
                     /translation="MPHQIKPVLAERYPLAFATGVADFVLIVVSSFLAFYLRFGHLAM
                     DQWYLLATFSSALTFIIFQIASHCYASWRGQGLLRPIGRLVGAWTSAVAFIFVVLVLL
                     KFGHYYS"
BASE COUNT          530 a          260 c          309 g          428 t
ORIGIN      
        1 ctggagtagt aatggccaaa cttcagtaac accagcacaa caaaaataaa agccactgca
       61 ctcgtccagg ccccgactaa acgacctatg ggtctgagca gcccctgacc acgccaagag
      121 gcataacaat gactggcaat ctgaaaaata atgaaggtca gggctgagga aaacgtcgcc
      181 agcagatacc actgatccat ggccagatgg ccaaaacgca aataaaaagc cagaaaagag
      241 ctcacgacaa taagcacaaa gtcagcaacc cctgtggcaa aggccagggg atatcgttcg
      301 gcaaggacgg gttttatctg gtggggcata ggtgagtgcg atgctttatt gcttaaacct
      361 gtgattttaa caggataaaa accacaagaa tgatttgcta tgctttttca gaatcaagtg
      421 agtttatgtc tatcagtgat aaaaaacata tgaacataaa aaaagtatta ccaataatgg
      481 tagacggtat ggatgttgcc aaaatcaata ataaagcaac ctggattttc ctttttgagt
      541 atttcaatag gatcgttaac gggataaaaa ataacagcaa tcctggccag cccagccaat
      601 accctatttc tacaataaaa gaatgaggat aagaaaaaag caactttatt tgttgaaatg
      661 aagcaccccc tatataagag tgatagtcat atagtttatg tgttcctagg ccgatttttg
      721 gcagttgttt caaaaagtgc aagtatgagc ttaatcttac agacatagag ctatctgata
      781 atattccttt atttagtatg tctataaggg agtatagtct attaattatt ctgtctaggg
      841 cagagttgtc tacaaaagta gatagggtat aaataaatat aatgcttgca aaaagcagga
      901 gaatatactt agctattttg attggacgtg gtctaagatt aattatagtt attgctataa
      961 ataatataat agcggatctt gatcctccaa ataccaagac taaagaaaat ataattaaag
     1021 aaagtcttag ctgtactatc gtgagatgtt tttttagtcc tagtagaata aaagataaga
     1081 gcaagattga ggcgctcata ttgttggggt tatagaatgt tcctgaaata aagtttctat
     1141 gccgatattc ctcgactata gggaacccat aatcgaatgt gtaagtgaaa aattgaccta
     1201 tacaaattat cagctggcca atggcaaatg ctattattat gtttgttaca gtttgtatat
     1261 ttatttccaa gttatcctgc ttatagtgaa ataccctaat tgcggttaga gccagaacag
     1321 gaaggaggga tagattaaag gtaatatagt tcagagcttt ttcctcgaaa ggatgcccgt
     1381 agtatgcaaa cagggataga atagtgaatg gtaaaaataa taatgcctct atgattggct
     1441 tttctcttct cgtttaaagc tccatatttc ataagctact acatacagtc ccgcaactat
     1501 tattggcaga taggcgattt tcccaga
//
LOCUS       DATGSI010000348         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_167375, whole genome shotgun sequence.
ACCESSION   DATGSI010000348 DATGSI010000000
VERSION     DATGSI010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167375"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VK031_05435"
     CDS             <1..290
                     /locus_tag="VK031_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53391.1"
                     /translation="NAQNLRGKHHHYADLSLGLQGRWLLTEDKQQVRIHHSLKYHRWM
                     GSHPLPKGHISAQIGLELQPNPQWQFGLNTQTRIGPGLRDQAISFNLTGVF"
     gene            424..576
                     /locus_tag="VK031_05440"
     CDS             424..576
                     /locus_tag="VK031_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53392.1"
                     /translation="MKRSAEQDKLDRELLMVGVTVRGLKGLWFAAKRGDYSAQRRLFQ
                     GILYVN"
     gene            complement(750..2051)
                     /locus_tag="VK031_05445"
     CDS             complement(750..2051)
                     /locus_tag="VK031_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53393.1"
                     /translation="MVERESLIQARKNAHTIGLVLLIITLSPSFPNLVGRSLLPSIIT
                     LTTYSLLVAYLFISKKLIIRIEIILFWILQYVFLLSTALPSDENIIPSDFTELLIPLS
                     SIFITIYLYSIFKETSEREILEINKIIYRYICFIILIILSISILEFIGLEINTLYILY
                     KPYEKYLGSTRVVTFFLYPYYLTFFLLTALCFILPVMSYTRSLFSFIIFLCTLTAIIL
                     AQSQSGFTSALLITFLYFFINNPNYINLFIRSAFLILIVIASIYLAPISIKWLASNFD
                     LYTFRAMNRIINSPETSGTLNVRLNQIIFAIQESYNNYLIVGAGWGRNAYLESWISYL
                     IHKYGVLYTFLLVLGWAYMIYKLFIKYKLYYQERSIISYYLKSFLVFIAVLPISLSSA
                     FLLFTHKLTLLFYFIIMLSLHIIDKPFIKKKTNLQQHNSMS"
BASE COUNT          806 a          383 c          467 g          725 t
ORIGIN      
        1 tcaacgccca gaacctccgc ggcaagcacc accactatgc ggatctgagc ctggggctac
       61 aaggccgctg gctacttacc gaggacaaac agcaggtgcg catccaccac agcctgaagt
      121 accaccgctg gatgggcagc cacccgctac ccaaaggtca catcagtgca caaatcggtc
      181 tggagctaca gcccaaccca cagtggcagt ttggcctgaa cacccaaacc cgcatcggcc
      241 ccggactcag ggatcaagcc atcagcttta acctgacggg ggtattttag ggaaaggagg
      301 agagtaagaa aagtagaggt tgaaaaacac gccgcaagag tcctctgcga gacgtaggga
      361 tatcatgtaa aatcacccct acgttttgta acgactcttg cggtaaaaac gaggaggatc
      421 gccatgaaaa gaagcgctga acaggataaa ttagacaggg agctcttgat ggtaggagtg
      481 actgtaagag ggttaaaagg gctttggttt gcagctaaaa ggggggatta cagcgctcaa
      541 aggaggcttt ttcaggggat tctatacgta aattgaagtg cgcaaacagc tgggttatgg
      601 tcattggtta tttattcata gagaaaaata accagcgcgt attcaacaag cttcaaccaa
      661 gtgcgatata aaaaagagat aaattgccgt tcatacgcac aattttaagg ttattcatat
      721 ttttctttgg ataatttttt tggcgactgt tagctcatac tattgtgttg ctggaggttt
      781 gtcttcttct tgataaaagg tttatcaata atgtgcaaag aaagcataat aatgaaataa
      841 aataatagag ttaatttatg agtgaacaat aaaaaggcac ttgataaaga aattggtaga
      901 acagctataa atacaagaaa tgattttaag taataagaaa taatgcttct ttcttggtag
      961 tacagtttgt actttataaa tagcttataa atcatgtaag cccagcctag aactagtaaa
     1021 aaagtatata aaacaccata tttgtgtatt aaatatgata tccaactttc caagtaagcg
     1081 tttcttcccc agcctgcacc aacaattaag taattattgt agctttcttg gatggcaaat
     1141 attatttgat ttagtctgac atttaaagtt ccgctggttt caggagaatt aataatccta
     1201 ttcatagcgc gaaacgtgta gagatcaaaa tttgatgcaa gccattttat agaaataggg
     1261 gctaagtata tagatgcgat tacaataagt attaaaaaag cggatctaat aaaaagattt
     1321 atataattag gattgtttat aaaaaagtat aggaatgtaa taagtaaggc tgaagtaaaa
     1381 cctgactggc tttgcgctag tatgatagca gtcaaagtgc aaaggaaaat aataaagcta
     1441 aataagcttc ttgtatagct cattactggc aaaataaaac acagagcagt tagtaagaaa
     1501 aatgttagat aatatgggta tagaaaaaaa gtaactactc gtgtactgcc taaatatttt
     1561 tcatatggtt tatataatat atacaatgta tttatttcaa gccctatgaa ttcaagaatg
     1621 gatatgctta atattattaa aataataaag catatatatc tatatattat tttgtttatc
     1681 tccaatatct ccctttcaga tgtttcttta aagatagagt atagatatat tgtgataaat
     1741 atactgctta aagggattaa tagctcagta aaatcagaag gtattatatt ctcatctgaa
     1801 ggcaaggctg tactcagtaa aaaaacatat tgtagaatcc agaatagaat aatttctatt
     1861 cttattatta gtttttttga tataaataga tacgcaacta gcaatgaata ggtcgtcaaa
     1921 gtaattatac tgggcagtaa gcttctgccg acaaggtttg gaaaacttgg gctcagagta
     1981 ataataagga ggacgagccc tatagtgtgc gcattttttc tcgcttgaat taagctttcc
     2041 ctttcaacca ttttgtgctc attatctatt aagtgtttat aattttttat tgctatcaaa
     2101 cagctgtcta ttaacttgta tggaacgatt gtgcataagt aaattgtata tttatttatc
     2161 tgttgtttgt gttgcaacct gagtatatag atgcgtatga tctattatgt taaggtatgt
     2221 tttttacttt tatagcatca agtattatta aataaggaaa tatgcatctt taaaataacc
     2281 tcagctgcat attcccgatc tattttttct ttggctttag cacccatatt atgggcattg
     2341 acgtctgagt ataaagtaag catgcaccgt gcgatatctt c
//
LOCUS       DATGSI010000349         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_22441, whole genome shotgun sequence.
ACCESSION   DATGSI010000349 DATGSI010000000
VERSION     DATGSI010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22441"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..980
                     /locus_tag="VK031_05450"
     CDS             195..980
                     /locus_tag="VK031_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HLS53394.1"
                     /translation="MSRNVNFLEQPHASSNIKKVIGVVSGKGGVGKSLVTSMLAVTMN
                     RLGYNSAVLDADVTGPSIPKVFGLTDRAKGTDDGILTNKSKTGIDIMSVNLLLKKETD
                     PVMWRGPIITSTIKQFWTDVMWGNVDFMFIDMPPGTGEVALTVFQSIPVDGIVVVTSP
                     QELVSMIVAKAVRMAEMMGIPIIGLVENMAYFQCDDCGKEHKIFGESHIDEIAKDHNL
                     EVLAKLPIMPEIAQLCDQGRIEDFNGTWLNDTAIVLEKMLLKG"
     gene            complement(1012..2337)
                     /locus_tag="VK031_05455"
     CDS             complement(1012..2337)
                     /locus_tag="VK031_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53395.1"
                     /translation="MKGKDVNERFLELIKRDQAKYGKDYQLFVDRVKNSSAQYRGKPV
                     PTLYNPMFITEDNLEDFRKIGETMMAIANKITDRYINDREFRKKFGFPKLIEDLIEID
                     NGYDIHVPIGRFDIFYKDGETFKFCELNTDGSSAMNEDNTLAKIMLESEGAKDFKKDY
                     ELEYFELFDTWVEDIIKIFHKYDPNKEKPNVAIVDFTESGTTPEFEEFKKRFIRQGYN
                     CIIADPRNLEYKDGKLWHKDYKIDLVYRRIVTFELIDKADEIPDFIAAYKDKAFCCVG
                     SIRSQVIHNKIFFKILHDEDTLEGLNQKERDFVKRHIPDTGIFAGDRKTFNKVLNNKD
                     KYIMKPMDLNASQGVYAGRDFSQSEWEKKLKRSWKTDYLYQEFVEPYTRDFLIYKDGE
                     FKEETYGSIIGLYMYNEKLAGIYSRVGPNNIISGATEYYTLPSILVKNT"
     gene            complement(2337..>3183)
                     /locus_tag="VK031_05460"
     CDS             complement(2337..>3183)
                     /locus_tag="VK031_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HLS53396.1"
                     /translation="YFKTRGSHAHNMMKGTAALQFAMDFKSEKDYVKKFRVVNALSPV
                     LFAIFENAYYFEGEVCAKHNIRSHIWVNCDSDRSGLVEGALEDDFGYERYAEYILNRP
                     PIFINKDGEEIFTGRKKVREVFDPENYQVEELEHILTMFFPDVRTKGYIEIRMMDAVP
                     YPLNFAAMALFKGLIYDEDNLEKLYKYTKDITIEDVDKAKAEMLDLGMEAKLRDKSLL
                     EIGRYITRLSQEGLSPEEVHYLKPLGDMLAEGLNPYEITKSKVKLGKKEALSWCILNN
                     IVEEL"
BASE COUNT          914 a          629 c          506 g         1134 t
ORIGIN      
        1 tataaataga tttatcagaa tctaaatcca gcaagttatc acttccctta gtttactaat
       61 tggttcattg gttagttacc tgtgtaacta agtatatagg ttctagtagt tactgtcaag
      121 ctttttagga aaatcataaa agtttcacat ttttaatttc catagtgtta taatagttcc
      181 aaaggaggtt ttttatgtca agaaatgtaa actttctaga gcagcctcat gcttctagta
      241 atataaagaa ggttattggt gtagtaagtg gtaaaggtgg agtaggtaag tctttagtaa
      301 cttccatgct tgctgttacc atgaatagat taggctataa ttctgctgtt ttagatgcag
      361 acgttactgg cccttccata cctaaggttt tcggccttac tgaccgagcc aagggaacag
      421 atgatggtat tttgaccaat aaatctaaga caggtataga tattatgtct gtaaacctgc
      481 tattaaaaaa ggaaacagac cctgttatgt ggcgaggccc cataattact agcaccatta
      541 aacaattttg gacagatgtt atgtggggta atgtggactt tatgtttata gatatgcctc
      601 cagggacagg ggaggtggcc ttaacagtct ttcaatctat tccagtagat ggaatagtgg
      661 ttgttacatc tccccaggag ctggtctcta tgattgtggc aaaggctgtc aggatggctg
      721 aaatgatggg tatacctatt attggtttgg tagaaaatat ggcctatttt cagtgtgatg
      781 attgtggtaa ggagcacaaa atctttggcg aaagccatat agatgaaatt gccaaggatc
      841 ataatttaga agtcctagcc aagttgccta ttatgcctga aattgctcag ctttgtgacc
      901 agggcaggat tgaggacttt aatggcacat ggcttaatga tactgccata gttttagaaa
      961 aaatgttgtt aaagggataa aaaaattgcc aggtttttaa cctggcaatt tttaggtgtt
     1021 ctttacaagt atactaggta aggtatagta ttctgtagcc ccagatatta tattgtttgg
     1081 ccctactctg gaataaatcc cagccaactt ttcattatac atatatagac caattatgct
     1141 accataagtt tcttctttaa attctccatc cttatatata agaaaatccc tagtataagg
     1201 ttctacaaac tcctgatata agtaatctgt tttccaggat cttttaagtt ttttctccca
     1261 ttctgattgg ctaaaatctc tacctgcata tacaccttga gaagcgttta agtccatagg
     1321 cttcataata tatttatcct tattatttag gaccttgtta aatgtttttc tgtctcctgc
     1381 aaatatgcct gtgtcaggaa tgtgtctttt tacaaaatcc ctttctttct gatttagccc
     1441 ttccaaggta tcttcatcat ggagtatttt gaaaaagatt ttattatgaa taacttgaga
     1501 tctaatagag cctacacaac agaaggcctt gtctttatag gctgctataa agtccggtat
     1561 ttcatcggcc ttatcaatca actcaaaggt gactatcctt ctatatacta aatctatttt
     1621 gtaatccttg tgccatagct tgccatcttt atattctaaa ttcctaggat ctgctattat
     1681 gcaattataa ccttgcctta taaatctttt cttaaattct tcaaattctg gtgtggttcc
     1741 gctttctgta aagtctacta tggccacatt tggtttttcc ttatttggat catatttatg
     1801 aaatattttt ataatgtctt caacccaagt atcaaacaac tcaaagtatt ccaactcata
     1861 gtcttttttg aaatcctttg ctccttcaga ttctaacata atcttagcca aagtattgtc
     1921 ttcattcatg gcagaagagc cgtctgtatt taattcacaa aacttaaagg tctccccatc
     1981 cttataaaaa atatcaaacc ttccgatggg cacatgtatg tcataaccat tgtctatttc
     2041 tattaagtcc tctatcaatt taggaaaccc aaattttttt ctgaattccc tatcatttat
     2101 atagcgatca gttattttat tggctatggc catcattgtt tccccaatct ttctaaaatc
     2161 ttccaagtta tcttccgtaa taaacattgg attataaagg gttggtaccg gttttcccct
     2221 atattgggcc gatgagtttt taaccctatc cacaaacaat tgatagtcct taccatattt
     2281 ggcctggtcc ctttttataa gttctaaaaa tctttcgttt acatcttttc ccttcattac
     2341 aattcctcca ctatattatt aaggatacac caggataggg cctctttttt ccctagctta
     2401 accttgcttt ttgttatttc ataaggattt aagccttcag ccaacatatc gcccaaaggc
     2461 tttagataat gaacctcttc tggtgacaat ccctcctggc ttagtcgggt tatatatcta
     2521 ccgatttcta acaagctctt atctcttaac ttggcttcca tacctaaatc taacatttca
     2581 gcctttgcct tatccacatc ttctatggtt atatccttag tatatttata tagtttttcc
     2641 aaattatctt catcatatat aagtccctta aataaggcca tggctgcaaa gtttaaagga
     2701 tatggcactg catccatcat cctaatttct atatacccct tagtccttac atctgggaaa
     2761 aacatggtta atatatgctc taattcttcc acctgataat tctctggatc aaatacttcc
     2821 ctaacctttt tcctaccagt aaatatctct tctccgtcct tattaataaa gataggcggc
     2881 ctgtttaata tatattctgc atacctttca tagccaaaat catcttccag ggccccttct
     2941 actaatcctg acctgtcact atcgcagttt acccatatgt gggacctaat attatgttta
     3001 gcgcagactt caccctcaaa gtaataggca ttttcaaaga tagcaaataa aactggtgac
     3061 aaggcattta caactctgaa ttttttcaca taatcttttt cagacttaaa atccatggca
     3121 aattgtaaag ctgccgtccc cttcatcata ttgtgggcat gactgcctct ggtcttaaaa
     3181 taa
//
LOCUS       DATGSI010000350         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_121299, whole genome shotgun sequence.
ACCESSION   DATGSI010000350 DATGSI010000000
VERSION     DATGSI010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121299"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VK031_05465"
     CDS             complement(<1..567)
                     /locus_tag="VK031_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLS53397.1"
                     /translation="MKRKIYLVLCLILAVVYMNGCSKSTNINLVSREEGSGTRSAFIE
                     MVGLLEKSSKGNLEDLTSQEAIIQNSTEAIINTVAGDKNSIGYISLGSLNHRVKALKI
                     NDIPVSTENIKNNSYQIARPFNIVFKKDLKPLTQDFIDFILSQDGQNIINNEAYIPVD
                     IKNHNYSPSQNQGSLVIAGSTSIYPVMEK"
     gene            798..1430
                     /gene="tmk"
                     /locus_tag="VK031_05470"
     CDS             798..1430
                     /gene="tmk"
                     /locus_tag="VK031_05470"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HLS53398.1"
                     /translation="MGGLFIALEGPDGSGKSTILELIGDYLEKKGIEYIKTREPGGTP
                     IGEEIRDIILDEKNVNMGAETEALLYAASRSQHIHEKIIPALKEGKLVLCDRFVLSSL
                     AYQGIGRGLGIERVKSINDFALRGIYPDLILFFHIDPEVSLKRKTEKLGGDRLEQEGN
                     DFHKKVYDGYMKLIDLYPDKIKVIDASQSVEEVLSQSIGEIEKLLISEEG"
     gene            1432..1758
                     /locus_tag="VK031_05475"
     CDS             1432..1758
                     /locus_tag="VK031_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HLS53399.1"
                     /translation="MKLIVAIIQDQDAPSLIEDLTENDFGVTKLASTGGFLRAGNTTL
                     LIGVETDRVPDLLKIVENNCKTREITTSLLTVSMPGDTYIPYPLEVKVGGATLFILNV
                     ERHIKI"
     gene            1773..2057
                     /locus_tag="VK031_05480"
     CDS             1773..2057
                     /locus_tag="VK031_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HLS53400.1"
                     /translation="MKLIIAIVQDEYANKVIRGLMEEKIRTTKLSSSGGFLKSGNTTL
                     LIGAKEEDIDKIVDIIKARCKSTRVKEGKEEITVGGANLFIVDIDEQVKI"
BASE COUNT          817 a          294 c          433 g          661 t
ORIGIN      
        1 tttttccata actggataaa tagaggttga gcctgctatc actaaactac cttgattttg
       61 tgaagggcta taattatgat ttttaatatc tacaggaata taagcttcat tatttatgat
      121 attttgtcca tcctggctta agataaagtc aataaagtct tgagtcaaag gctttaggtc
      181 ctttttaaaa acgatattaa atggtcttgc aatttggtaa ctattatttt ttatattttc
      241 agttgaaaca ggaatgtcat tgatttttaa agctttaacc ctgtgattta atgagcctaa
      301 ggatatatat cctattgaat ttttatctcc agctacggtg ttaattattg cctctgtact
      361 attttgaata attgcttctt gagaagtaag atcctcaaga tttccctttg agcttttttc
      421 taataggcca accatttcaa tgaatgctga ccttgtacca gaaccttctt cccgactaac
      481 caaatttata ttggtacttt tgctacatcc attcatataa actactgcta atattagaca
      541 taagactaaa taaatttttc ttttcatatt atcctccctt tagatattaa taattaaata
      601 ataggctaga aatgtaaagt ctagtctaga gttcatataa agaatatgta aaataatatg
      661 taacataaaa actcatttaa cagttaaatt aagcaaagcc aatatcttga attatgagaa
      721 atagactaga aatagtataa attataggat ataataaaga ccaaacagaa gatggaagac
      781 aaaggagttg ctacttgttg ggaggattat tcatagcttt agaaggcccg gatgggtcag
      841 gtaagtcaac aattttagaa ttaattgggg attatctaga aaaaaaggga atagaatata
      901 taaaaactag ggaacctgga gggaccccaa taggagaaga aataagagat ataattttgg
      961 acgaaaaaaa tgttaatatg ggggcggaaa ctgaagcctt attatatgcg gcctcccgta
     1021 gtcaacatat acatgaaaaa attataccag ccctgaagga aggcaagctg gttttatgtg
     1081 ataggtttgt tttatctagc ctggcctatc aaggaatagg ccgagggctt ggaattgaaa
     1141 gggtaaaatc cattaacgac tttgcattaa ggggaattta tccagattta attctgtttt
     1201 tccatattga tccggaagtc tccttaaaga gaaaaactga aaaactaggt ggagataggc
     1261 tagaacagga agggaatgac ttccataaaa aggtatatga tgggtatatg aaattgatag
     1321 atctgtatcc agataaaatc aaggtgatag atgccagtca gtctgtagag gaagttttga
     1381 gtcaatctat aggagaaatt gaaaaattat taatttcaga ggagggctaa aatgaaactt
     1441 atagtggcaa ttattcaaga ccaggatgct cctagcttaa ttgaggattt aacagaaaat
     1501 gattttggag ttacaaaatt agcatctaca gggggctttt taagagcagg aaatactacc
     1561 ctactaatag gagtagagac tgacagggta ccggacttat taaaaattgt ggaaaacaat
     1621 tgtaagacaa gagaaataac tacctcttta ttaactgtct ccatgcctgg agatacatat
     1681 ataccctatc ctttagaagt taaggtagga ggagcaacat tatttatctt aaatgtagaa
     1741 agacatatta aaatatagag gaggcggttt ttatgaaatt gataatagcc attgtccagg
     1801 atgaatacgc aaacaaggta attagaggac taatggaaga aaagataaga acaacgaagc
     1861 tatcttcctc aggaggcttt ttaaaatcag gtaatactac cctgttaatt ggagccaagg
     1921 aagaggatat agataagata gtagatataa ttaaggccag gtgtaaaagt actagagtta
     1981 aagaaggaaa agaagaaatt acagttggag gagcaaatct gtttattgta gatattgatg
     2041 agcaggttaa gatataaagg agctattata tggactttag ccaagtaata ggacatgaaa
     2101 aaaacataga catattaaaa caagctataa aaaataagac cataagtcac agttatttat
     2161 ttgaaggtgc agaaggcata ggtaaaaaaa gagtagccta tgctt
//
LOCUS       DATGSI010000351         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_214470, whole genome shotgun sequence.
ACCESSION   DATGSI010000351 DATGSI010000000
VERSION     DATGSI010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214470"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1897
                     /gene="lepA"
                     /locus_tag="VK031_05485"
     CDS             89..1897
                     /gene="lepA"
                     /locus_tag="VK031_05485"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HLS53401.1"
                     /translation="MHEIKKSNIRNFSIIAHIDHGKSTLADRLIEKTGMLTSREMESQ
                     VLDNMDLEKERGITIKLKAARLRYQARDGEEYILNLIDTPGHVDFNYEVSRSLAACEG
                     AILIVDATQGIEAQTLANVYLALEEDLEIIPVINKIDLPSARPEEVKAEVEEVIGIDC
                     SDAPLISAKDGLNIEDVLEAIVKKVPAPKGEAEEPLKALIFDSFYDTYKGVVCYIRVF
                     EGSIRPGMEIKMMSSGKTFEVAEVGVNTNRHVELDKLQAGDVGYITASIKNVKEATVG
                     DTITDKNNPTDQPLPGYQEVVPMVYCGIYPAEGEDFNSVRDALEKLQVNDAALIFEPE
                     TSVALGFGFRCGFLGLLHMEIIQERLDREFDLNIIATAPSVIYKVVKNDGEVLEIQNP
                     TDMPPATEIESMQEPIVTADIMTPTDYVGAIMELCQDKRGTFLNMEYLEEDRVVMHYD
                     LPLNEVIYDFFDALKSRTRGYASFDYELKGYEESNLVKLDILINGESVDAFSMIVHED
                     KAYERGRSIAERLLDVIPRHQFAIPIQAAIGSKIIARETIRALRKDVLAKCYGGDISR
                     KKKLLERQKEGKKRMRQIGSVEVPQEAFLTVLKYDE"
     gene            1915..>2962
                     /gene="hemW"
                     /locus_tag="VK031_05490"
     CDS             1915..>2962
                     /gene="hemW"
                     /locus_tag="VK031_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HLS53402.1"
                     /translation="MCIYFNRGERVKNIGLYIHIPFCERKCFYCDFLSFTNREDLVEK
                     YIDYLKKELNLYKERLIDYEINTIFIGGGTPSHIEAKYIEKLLTYIYKNFNTARLEEV
                     SIEVNPKGLNKEKAKAYKRAGINRISLGLQTLNDNHLKSIGRLHRKEDFIVSYEILKD
                     LGFNNINVDLIFGLPGQTVEDVIYDVENICQLDIKHLSYYSLILEEGTLMHKLYEKGR
                     LDLPDEEEERQMYYTIVESLGKEGFIHYEISNFAKENYQCIHNKYYWEIKPYIGIGMG
                     SHSNFDGKRYWNSRRFDEYFAKLDQGQYPIIGQEKIDKKMEMAEYCIMGIRLIKGINR
                     TLFKERFRVDLEDIY"
BASE COUNT         1038 a          385 c          678 g          861 t
ORIGIN      
        1 gggctggtct tttgttttac ataaattcga ttatttgcta taattagcta ggattgtttg
       61 atttttctaa ggaggattgt aaaagttaat gcacgagata aaaaagagta atataaggaa
      121 tttttctata atcgcacata ttgatcatgg taaatctact ttagcagata gactgattga
      181 aaaaaccggc atgttgactt ccagggaaat ggaatcccag gttctagata atatggacct
      241 ggagaaagaa agaggtatta ccattaaatt aaaggcagct aggctacgtt atcaggccag
      301 ggatggagag gagtatattt taaacttaat agatactcca ggccatgtag actttaatta
      361 tgaagtttcc agatccttgg ctgcctgtga aggagctatt ttaattgtag atgctactca
      421 aggcattgag gcacaaacct tggccaatgt gtatttggct ttggaagagg atttagagat
      481 tatacctgtt atcaataaga tagatctgcc tagtgccagg cctgaagagg taaaagctga
      541 ggtggaggag gtaataggta ttgattgtag tgatgcaccc ttaatatcag ccaaggatgg
      601 cttaaatatt gaggatgttt tggaagctat agttaaaaag gtgccagctc caaaaggaga
      661 ggctgaagaa cctttaaagg cgctaatatt tgattctttc tatgatactt acaaaggagt
      721 agtttgttat attagggtat ttgaaggaag tataaggcca ggcatggaaa taaagatgat
      781 gtccagtggt aaaacttttg aagtggctga ggtaggagtt aatactaatc gacatgttga
      841 gttggacaaa ctccaggctg gagatgtagg ttatattact gccagtatta aaaatgttaa
      901 ggaagcaact gttggtgata ctattacaga taaaaacaat ccaacagacc aaccactgcc
      961 aggttatcaa gaagtagtac caatggtata ttgtggtata tatccggcag aaggggagga
     1021 ttttaactct gttagagacg ccttagaaaa actacaggta aatgatgcgg ccttaatttt
     1081 tgaaccagag acttcagtgg ccttaggctt tggatttagg tgtggttttt tagggctact
     1141 tcatatggaa attatacagg aaagattaga tagggaattt gacttaaata taattgctac
     1201 tgctcccagt gtaatatata aggttgttaa aaatgatgga gaggttttag aaatccaaaa
     1261 tcctacagac atgcctccgg ctacagaaat agaatctatg caggaaccaa ttgttactgc
     1321 agatataatg actccaacag attatgttgg ggctattatg gagctgtgtc aagataaaag
     1381 gggaaccttc ttgaatatgg aatatttaga ggaagacagg gtagttatgc attatgacct
     1441 gcctttaaat gaggttatat atgatttctt tgacgctcta aaatcaagga caaggggtta
     1501 tgcctccttt gattatgaat taaagggtta tgaagaatct aatttagtta agttagatat
     1561 tttaataaat ggagaatcag tagatgcctt ctccatgatt gttcatgagg ataaggccta
     1621 cgaaagaggc agaagtatag ctgaaagact tttagatgta ataccaagac accaatttgc
     1681 tatacctatt caggctgcca ttggctccaa gattatagcc agggagacca ttagggcttt
     1741 aaggaaagac gtattggcca agtgttatgg tggagatatc tctagaaaga aaaagttact
     1801 tgaaaggcaa aaggaaggaa aaaagagaat gaggcaaata ggttcagtag aggtgcctca
     1861 ggaggccttt ttaacagtac taaaatacga tgagtaaaat aaatgcacat gaagatgtgc
     1921 atttatttta atagaggtga aagagtgaaa aatataggct tgtacatcca tattcctttt
     1981 tgtgagagaa aatgtttcta ctgtgatttt ctatccttta caaataggga ggatctggtg
     2041 gagaagtata ttgattattt aaaaaaggaa ttgaacctat ataaagaaag gttaatagat
     2101 tacgaaatta atacgatttt tataggagga ggcacacctt cacatataga agcaaaatat
     2161 atagaaaagc ttttaaccta tatatataaa aattttaata cagccaggct ggaagaggta
     2221 tccatagaag taaatcctaa aggactaaac aaggaaaagg caaaagccta taaaagggca
     2281 ggtataaata ggatcagttt aggtttacaa actttaaatg acaaccatct taaaagcata
     2341 ggcaggcttc atagaaagga agactttatt gttagttatg aaatattaaa agatcttggg
     2401 tttaataata taaatgtgga tctgatcttt ggcctaccgg gacaaactgt ggaggatgta
     2461 atttacgatg tggaaaatat ttgccaacta gatataaaac atttgtccta ttacagctta
     2521 atattagaag agggcacctt aatgcacaag ttatatgaaa agggtaggct ggatttgcct
     2581 gatgaagagg aagaaagaca gatgtattac accatagtag aaagtttggg taaagaaggt
     2641 tttattcatt atgaaatctc taactttgcc aaggaaaatt atcagtgtat tcataacaaa
     2701 tactactggg aaataaagcc ctatattgga ataggcatgg gtagccattc caactttgat
     2761 ggtaaaaggt attggaatag cagaaggttt gatgagtatt ttgcaaaact agatcagggc
     2821 caatatccta ttataggcca agaaaagata gacaagaaaa tggagatggc tgagtactgt
     2881 attatgggaa ttaggctgat taagggtatt aataggaccc tttttaagga aaggtttagg
     2941 gtagatcttg aggatatata tg
//
LOCUS       DATGSI010000352         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_71442, whole genome shotgun sequence.
ACCESSION   DATGSI010000352 DATGSI010000000
VERSION     DATGSI010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71442"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VK031_05495"
     CDS             complement(<1..498)
                     /locus_tag="VK031_05495"
                     /inference="COORDINATES: protein motif:HMM:NF044960.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HLS53403.1"
                     /translation="MLPDIELNSMTLEELKKLMTELGEQAFRGEQLFIYFNKNNGLDI
                     NEATTLPLKFREEIREKTTVKEIKILEKLESKLDDTVKYLFLLEDNNVIESVLMKYEH
                     GYTICVSTQVGCRMGCRFCASTRGGKVRDLTASEMLNQVYMIEKDMNITINNIVLMGM
                     GEPLDN"
     gene            complement(549..>1778)
                     /gene="rsmB"
                     /locus_tag="VK031_05500"
     CDS             complement(549..>1778)
                     /gene="rsmB"
                     /locus_tag="VK031_05500"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HLS53404.1"
                     /translation="DQGLIREIVYGVLENRLYIDHVIAQVSKIRLNRIHFNILEILRI
                     GIYQIMFMDKIPDRAAVDESVKLATKYGHKGTVGYVNGVLRNIVRRKETFEQINVKDQ
                     IEYISIKYSHPLYLVKRWVHEFGLQFTIDLCKANNQRPALNIRVNTLKTSRDRLKKKL
                     EEKGFLCVVGNYANDCLIIENPQTITETEEYKEGLFFIQDESSMLVAQIMDPDEGSTI
                     LDLCAAPGGKSTHLAQKMNNKGQILSRDIHQHKIKLINNNAKRLGIEIIRASIFDALK
                     RDKELLKKVDYCLLDAPCSGFGIIRRKPEIKWNRKEKDIKELSKLQEKMLDVAKDYIK
                     VGGSLIYSTCTIEKEENMEVINKFLKNNPHFSLANISDKIENKENLETLDKGYIQLYP
                     NIHGTDGFFIAKMIRER"
BASE COUNT          505 a          366 c          218 g          689 t
ORIGIN      
        1 gttatctaag ggctccccca ttcccataag tacaatatta tttatggtta tattcatgtc
       61 tttttcaatc atataaactt gattcaacat ttccgaggca gttaagtccc taacttttcc
      121 acccctagtg gaagcacaaa acctacatcc cattctacag cctacttgag tagaaacaca
      181 tatggtgtaa ccgtgctcat atttcattaa aacactctca attacattat tatcttccaa
      241 tagaaataga tatttgactg tatcatctag cttggattct aatttttcca atatctttat
      301 ctccttaaca gtggtctttt cccttatctc ttctcgaaat ttcagtggaa gtgtggtagc
      361 ttcatttata tctaaaccat tatttttatt aaaataaata aataactgct ctcctctaaa
      421 ggcctgctct ccaagttcag tcatcaactt ttttaattct tccaaggtca tactgtttaa
      481 ttctatatca ggcaacaaat ttcaccacct aacctctaag ttattataca taataatctt
      541 gtaataatct atctttctct aatcattttt gcaataaaaa atccatctgt cccatgtata
      601 ttgggatata attgaatgta tcctttatct aaggtttcta gattttcttt gttttctatc
      661 ttatcactaa tatttgctag ggaaaagtgg ggattgtttt ttaaaaattt gtttattact
      721 tccatgtttt cttctttttc tatggtacag gtgctgtaga ttaagctacc acctacctta
      781 atatagtctt tagccacatc cagcatcttc tcttggagct tagatagttc ctttatatcc
      841 ttttcttttc tattccattt aatctcaggc ttacgtctta ttattccaaa gcctgaacat
      901 ggggcgtcta gtaaacaata atccactttt ttaagtaatt ctttatccct ctttaaggca
      961 tcaaagatgg aagccctaat aatttcaatg cccagccttt ttgcattatt atttattaat
     1021 tttattttgt gttgatgaat atccctactt aagatctgcc ccttgttatt cattttctgg
     1081 gccaaatgag ttgacttacc accaggggct gcacataggt ccaatatagt tgacccttca
     1141 tctggatcca tgatttgagc aaccaacata ctgctttcat cttggatgaa gaataatcct
     1201 tccttatatt cttctgtttc tgtaatagtt tgagggtttt caataattaa gcagtcattg
     1261 gcatagttgc ccactacaca aaggaatcct ttctcttcca atttcttttt caatctatct
     1321 ctgcttgttt ttaaagtatt aaccctaata tttaaagccg gcctttgatt attagccttg
     1381 cacaaatcta tggtaaattg aaggccaaat tcatgtaccc acctttttac taaatataaa
     1441 gggtgggagt atttaatgga aatgtactct atctgatcct taacatttat ttgttcaaaa
     1501 gtctcttttc ttctaactat atttcttaat actccattta catatcctac tgttccttta
     1561 tgaccatatt ttgttgccag ctttacagat tcatctactg ctgccctgtc tgggatctta
     1621 tccataaaca taatctgata tattccaatc cttaggatct ctaaaatatt aaaatgaatt
     1681 ctatttaacc taattttaga aacctgagca ataacatgat caatatatag cctgttttct
     1741 aaaaccccat atactatctc tctaattaat ccttgatc
//
LOCUS       DATGSI010000353         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_306180, whole genome shotgun sequence.
ACCESSION   DATGSI010000353 DATGSI010000000
VERSION     DATGSI010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306180"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /gene="citG"
                     /locus_tag="VK031_05505"
     CDS             <1..798
                     /gene="citG"
                     /locus_tag="VK031_05505"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:TIGR03125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HLS53405.1"
                     /translation="PKPGLVDRHNSGAHEDMDFFTFLNSSSSLYPYFYQCSREGLYFQ
                     GNNYTSLLKNIRPIGIEAERIMFASTQGVNTHKGLIFSLGILAAASGSLYKENRSLSP
                     KTLSDRVQEISQGVTKELKEAKSSLTYGEVLYKKYGITGIRGEVEAGFPTVLEYSLPV
                     FNRLITEDKYHINTILVHTLLYLIAHTEDSNILGRHNMQKLNYAQKEAKEAIKLGGYL
                     SLAGREKVKEMDESFIRQNISPGGAADLLAVTLMFHQLDNLDSEEII"
     gene            906..2291
                     /locus_tag="VK031_05510"
     CDS             906..2291
                     /locus_tag="VK031_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HLS53406.1"
                     /translation="MTIKYVPEPFRIKMVEPIKMLSREEREERIKEAKYNLFGLKGED
                     VYIDLLTDSGTNAMSDAQWSGVMIGDEAYAGGKSYYRLLDAGRDIFGYKFIQPVHQGR
                     AAEKVLFELLLEEGKYAISNMFFDTTRAHVEIAGARAIDCVVEEALDPTLEAPFKGNM
                     DVEKLERLINEYGKDKVGLVVMTITNNSAGGQPVSVQNMREVAEVCKKYEIPLCIDAA
                     RFAENSYFIKLREEEFKDKSIKEITKEIFSYADMFTMSAKKDGIVNMGGILGIKEDEA
                     LFQKVKENTISFEGFVTYGGLSGRDLEALAIGLYEGIDENYLAYRHGQMQYLASKLDK
                     AGIAYQSPVGGHGIFVDAKAMFPHIPYNEFPGQALAVELYLEAGIRTCDIGSYMLGND
                     PDTGEQLEADFEFTRLAIPRRVYTQAHFDVMADALIAIKERAEHVRGYRIVWEPAILR
                     HFQAHLEPLEK"
BASE COUNT          812 a          369 c          525 g          627 t
ORIGIN      
        1 cctaagccag gcctagtaga taggcataat tcaggtgccc atgaagacat ggattttttt
       61 acttttttaa atagtagttc ttcactttat ccctactttt accaatgtag tagggaagga
      121 ctttattttc agggaaataa ttatacaagc cttttaaaaa atattaggcc cataggcata
      181 gaggctgaaa ggataatgtt tgcttctacc caaggagtaa atactcataa gggattaatt
      241 ttttccctag ggattttggc agctgcatca ggttccctat ataaagaaaa tagaagcttg
      301 agtccaaaaa ctttatcaga tcgggtacaa gaaattagcc agggggttac caaggagtta
      361 aaagaagcta agagtagctt aacttatgga gaggttctat ataaaaaata tggcataact
      421 ggtataagag gagaggtgga ggcaggcttt cctacagttt tggaatattc cttgcctgta
      481 tttaatagat taataactga agacaagtat catataaata caatcttagt tcatactctc
      541 ctgtatctaa ttgcccatac agaagattcc aacatcttag gcagacacaa tatgcaaaaa
      601 ttaaactatg cccaaaaaga ggccaaggag gccatcaagc taggaggtta tttaagctta
      661 gcaggcagag aaaaggtaaa agaaatggat gaaagcttta ttagacaaaa tataagtcct
      721 ggaggagcag cagacctttt ggctgtaaca ttgatgttcc accagctaga caatctagat
      781 tcagaagaaa taatataaaa ataaattgac aagctattaa gctttggtag aatgaagatg
      841 aaagacaata ttaaacattc ctaaatttag gaatattcta actacttaaa tgagggggta
      901 ataatatgac tatcaagtat gtaccagaac catttaggat taaaatggtt gagcccatta
      961 agatgttaag cagggaagaa agggaagaaa gaataaaaga agctaagtat aatctttttg
     1021 ggctaaaggg agaggatgtc tacatcgatc tattaacaga cagtggtacc aatgccatga
     1081 gtgatgctca atggtctgga gtcatgattg gagatgaggc ttatgcagga ggtaagagtt
     1141 actatcggtt actagatgca ggtagagata tttttggtta taaatttatc caacctgttc
     1201 atcagggccg ggcagcagaa aaggtcttat ttgaactttt attggaagaa ggtaaatacg
     1261 ccatttctaa tatgttcttt gatactacaa gggcccatgt ggaaatagca ggagccaggg
     1321 ccatagactg tgtagtagaa gaagccttag atccaacctt agaggcaccc tttaaaggca
     1381 atatggatgt agagaaacta gaaaggctaa tcaatgaata tgggaaggat aaggttggcc
     1441 tggtagttat gactattacc aataattctg ccggtggaca acctgtttct gttcaaaaca
     1501 tgagagaggt tgcagaggtt tgtaaaaagt atgaaatacc cctatgtata gatgcagcta
     1561 gatttgcgga aaactcctac tttatcaaat taagggagga agaatttaaa gacaaatcaa
     1621 taaaggaaat aaccaaggaa atcttcagtt atgcagatat gtttaccatg agtgccaaaa
     1681 aagatggaat tgttaatatg ggtggcatct taggcataaa agaagatgag gctttattcc
     1741 aaaaagtaaa ggaaaataca atttcttttg aaggctttgt tacctatggt ggactatcag
     1801 gtagggattt ggaagcccta gccataggcc tatatgaagg aatagatgaa aattatttag
     1861 cctatagaca tggacaaatg cagtatttgg ccagtaagct ggataaggca ggcatagcct
     1921 atcagtcccc tgtaggaggt catggaatct ttgtagacgc aaaggccatg ttccctcata
     1981 ttccatataa tgagtttcca ggccaggccc tagcagttga actatatttg gaggcaggaa
     2041 ttagaacctg tgatataggc tcctacatgc taggaaatga tccagataca ggagagcagc
     2101 tagaggcaga ctttgaattt acaagactgg ccatacctag aagggtatat acccaggccc
     2161 actttgacgt aatggcagat gccttaatag ccataaaaga aagggcagaa catgtaagag
     2221 gttataggat tgtttgggag ccagccatat tgcgtcattt ccaggcgcac ctagagcctt
     2281 tagagaaata agacataaat tcccgtaaaa tacctatata ggggaagtcc ctt
//
LOCUS       DATGSI010000354         1326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_545473, whole genome shotgun sequence.
ACCESSION   DATGSI010000354 DATGSI010000000
VERSION     DATGSI010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545473"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..551)
                     /locus_tag="VK031_05515"
     CDS             complement(3..551)
                     /locus_tag="VK031_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HLS53407.1"
                     /translation="MDLKQIENDVKQISLELLNAANLQAEDAIVIGGSTSEILGKDLG
                     SATNIEVARVVVKTIVDILRENKIYPVIQACEHINRALVVEEEYAIKHNLDPVNVIPT
                     LSAGGGFATAAMEIFSRPVVVEHVAVQAGIDIGDVFIGMHIKGVGVVVRSDIKEIGKA
                     HISMIRTRPKLIGGARSKHYKT"
     gene            729..>1326
                     /locus_tag="VK031_05520"
     CDS             729..>1326
                     /locus_tag="VK031_05520"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HLS53408.1"
                     /translation="MKRSIRFILLSLVLGLILANFTACAANMKESSDESGQEIASDRS
                     QLGIDNMGSSPEESVDQEENNKIITTMHLSFETNQFDATEKKLNKLVGKYKGYIESSD
                     ISHDENYAAKKYKYGEFKIRIPKNSLASFRDELKGVGNLIYESTNKDDVTKTYKDTES
                     RLKVITTKEERILSLLEKADKMEDIIALENQLTEIIYEK"
BASE COUNT          429 a          251 c          207 g          439 t
ORIGIN      
        1 ccctatgttt tataatgttt agaacgagcg cctccaatta gtttaggtct agttctaatc
       61 atgctaatat gagcctttcc tatttccttt atatcacttc taactaccac acctacacct
      121 tttatatgca tacctataaa aacatcccca atatctatgc ctgcttgtac agctacatgc
      181 tctaccacca caggtctgga aaatatctcc attgcagcag tggcaaatcc tccacctgca
      241 cttaaggttg ggatcacatt aaccgggtcc aaattatgtt ttatggcata ctcttcttcc
      301 acaaccaggg ccctatttat atgctcacag gcctgaataa ctgggtatat tttattttcc
      361 cttaaaatat ccactatggt tttaactact actcttgcca cctctatatt tgtggcagaa
      421 cctaaatcct ttcctagtat ttcactggta ctaccaccta ttacaattgc atcttccgcc
      481 tgtaaattag ctgcatttag tagttctaag cttatctgct taacatcgtt ctcaatttgt
      541 tttaaatcca tacttctccc tccttccctc ttactattgt accatatcat gtatatatat
      601 tattcctctt aatataaata tacccatata ctgaatttaa ttatatttct gtaagttaat
      661 agaatattta acatttttct atcctatctt tatttatctc atatataatt ggtttaagga
      721 gggattttat gaaaaggtca ataaggttta tactattgtc actggtcctt ggcttaattt
      781 tagccaactt tacggcctgt gctgctaata tgaaagaatc atctgatgaa tctggtcaag
      841 agatagcgtc tgataggtca caattaggta tagataatat gggatccagc ccggaagagt
      901 ctgtagacca ggaagaaaac aataaaataa tcaccaccat gcacttatcc tttgaaacaa
      961 atcaatttga tgctacagag aaaaagctta ataagctggt tggaaaatat aagggctaca
     1021 tagaaagttc tgatatttct catgatgaaa attatgctgc taaaaagtat aagtatggag
     1081 agtttaaaat taggattcca aaaaacagtt tagctagctt tagggatgaa ctaaaaggtg
     1141 ttggaaatct aatttatgaa agtactaata aggatgatgt gaccaaaact tataaggata
     1201 ctgaatccag gcttaaagtt ataactacta aggaagagcg gatactgagc cttttagaaa
     1261 aggcagataa aatggaagat attatagcct tagagaatca acttacagag attatttatg
     1321 aaaagg
//
LOCUS       DATGSI010000355         1477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_257076, whole genome shotgun sequence.
ACCESSION   DATGSI010000355 DATGSI010000000
VERSION     DATGSI010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257076"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VK031_05525"
     CDS             <1..229
                     /locus_tag="VK031_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HLS53409.1"
                     /translation="GNPGMATAGSGDVLTGIIVSLLGQGLKPFDSARLGVYLHGLAGD
                     LAKEDKGEFGLIAGDILEKIPLGIMKYMEGC"
     gene            366..803
                     /locus_tag="VK031_05530"
     CDS             366..803
                     /locus_tag="VK031_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53410.1"
                     /translation="MKINFTKKQYRLLLDLVYIGRFVINGIRTSDQILEEYEEIQQYV
                     YSFAKKMGCGDLVEYNRESNMFYETNEFDNMVMEQFIEEYDAEVFWTELATGLAERDV
                     MKGSTGKDEIKPEELIMKIWAREEEYNEEFYENGLDNVKVDMK"
     gene            829..1413
                     /locus_tag="VK031_05535"
     CDS             829..1413
                     /locus_tag="VK031_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HLS53411.1"
                     /translation="MCGRYSLDYDLKNIIIRYKATKSIKEFQARDEIFPTDLAPVVVR
                     DQENMIAIMKWGFAPGFAKQPLINARGETIDIKPTFKNSFYNKRCIIPVSSFFEWENI
                     DGKKIRRRISISNEKIFSLAGLYDVFQDKEGKEYAAYTIITTEANKEIKVIHHRMPVI
                     IPKDMENIWLDRNNRNIKELKDLLRPYKKDMVIL"
BASE COUNT          567 a          172 c          330 g          408 t
ORIGIN      
        1 tggcaatcca ggtatggcca cagcaggtag tggagatgta ctgacaggaa taattgtaag
       61 cctactgggg caaggcctta aaccctttga cagcgcaagg cttggagtat atttacatgg
      121 cctggcaggg gatttggcaa aagaagataa gggtgaattt gggctaatag ctggtgatat
      181 actggaaaag ataccattag gtattatgaa gtatatggaa ggttgttaaa atgatagttt
      241 cgcattttat atagccaatg aaataagtaa aaaagaattg ggttaataat attttctaca
      301 catatgggta caagaatata gtatcaccct ttaaaataat aatgaaatga aaaggagaca
      361 aacacatgaa aattaatttt acaaaaaaac aatacaggtt acttctagat ttagtatata
      421 tagggaggtt tgtaattaac ggcattagaa cttctgacca aattttggag gaatatgagg
      481 agatccagca gtatgtatat tcctttgcta agaaaatggg ttgtggagac ttagttgaat
      541 ataatagaga aagtaatatg ttttatgaga ctaatgaatt tgataatatg gtaatggagc
      601 aatttattga agaatatgat gctgaggtat tttggacaga gctggctacg ggtttggctg
      661 agagagatgt aatgaaaggc tcaactggga aagatgagat taaacctgag gagttaatta
      721 tgaaaatatg ggcaagggaa gaggaataca atgaggaatt ctacgaaaat ggattagata
      781 atgtaaaggt agatatgaaa taaaataaag ttttatgttg gtgataatat gtgtggtaga
      841 tactcgttag actatgattt aaaaaatata ataataagat acaaggctac aaaaagtata
      901 aaagaatttc aagctaggga tgaaatattt cctacagacc tagcacctgt ggtagtgcgg
      961 gatcaggaaa acatgatagc aataatgaag tggggatttg cacctggctt tgcaaaacag
     1021 cccttaatta atgctagagg agaaacaatt gatataaaac ctactttcaa aaattccttc
     1081 tataataaaa ggtgtatcat accagtcagt agcttttttg aatgggaaaa tatagatgga
     1141 aaaaagattc gaaggagaat ctctataagt aatgaaaaaa tattttccct ggctggactg
     1201 tatgatgtgt ttcaggataa ggaaggcaaa gaatatgctg cctacaccat cataaccact
     1261 gaagctaata aggaaataaa ggtcatccac catagaatgc cggtaataat acctaaagat
     1321 atggaaaaca tttggctaga tagaaataat agaaatataa aagaattaaa ggacctacta
     1381 aggccttata aaaaggatat ggtgatttta taatctttga aattgttaag tagatggcca
     1441 ctagggataa aagaaatctg gcataaactg tggccct
//
LOCUS       DATGSI010000356         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_71585, whole genome shotgun sequence.
ACCESSION   DATGSI010000356 DATGSI010000000
VERSION     DATGSI010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71585"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VK031_05540"
     CDS             <1..648
                     /locus_tag="VK031_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HLS53412.1"
                     /translation="MGFAGDNDEKFRMSANLDPHGRFEWGVPPASRGDYAFVQHMLYS
                     LAENGRMGVILPHGVLFRGASEGRIRKQIIDMNLLEAVIGLPEALFFGTGIPACIMLF
                     RKNRNRKDVLFIDASGEDNYEKDGNRNRLKEEAIEKIVETYNNYETIDKYSYVASLDE
                     IKENDYNLNIPRYVDTFEEEDPVDMEEVARNIANIKKELKEVEEEMARYLDELGL"
     gene            678..1175
                     /locus_tag="VK031_05545"
     CDS             678..1175
                     /locus_tag="VK031_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53413.1"
                     /translation="MSSSLNKSDFYYGTFLTKVLDNGNKPALVSKNDGRGIYKLTTNK
                     EDYIVYIKYATNTNKNHKRWTFNYTANNIEEIKKYMKEDKNIIFAYVCAYNDLRNSEI
                     AIANLEELKKCINPKCKRNSVNRVSIYKKPYSPVLRMYGTKRADIMNGRDNTIHLKRN
                     RINEL"
     gene            1189..>1649
                     /locus_tag="VK031_05550"
     CDS             1189..>1649
                     /locus_tag="VK031_05550"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HLS53414.1"
                     /translation="MKEEIRERIEMINKGQVPEGYKKTKVGIIPEDWEESFLENLCDI
                     STGDKDTKDRKEDGKYPFFVRSNNVESIDSYSYDGEAILTAGDGVGTGKVFHYVNGKF
                     DYHQRVYCLREFRDINGKYLYYYFSWNFIKQVVKYSAKTSVDSVRKEMLTK"
BASE COUNT          661 a          189 c          356 g          443 t
ORIGIN      
        1 atgggctttg ccggtgataa tgatgaaaag tttagaatgt cagccaacct ggatccacac
       61 ggcaggtttg aatggggggt accgccagca tcaaggggag actatgcctt tgttcaacat
      121 atgctctatt ccttggcgga aaatggacgg atgggagtta tcctacctca cggagttctt
      181 tttagagggg ccagtgaagg aaggataaga aagcaaatta tagatatgaa cctattggaa
      241 gctgtaatag gcctacctga agcccttttc ttcggcacag gaataccagc ttgtattatg
      301 ttgtttagaa agaataggaa tagaaaagat gtcctattta tagatgcatc aggtgaagat
      361 aactacgaaa aggatggtaa cagaaacagg ctaaaggaag aagcgataga aaaaattgta
      421 gaaacctata ataattatga aactatagat aagtattctt atgtggcaag cctagatgaa
      481 atcaaggaaa atgactacaa tttaaatata ccaaggtatg tggatacctt tgaagaagag
      541 gatccggtag atatggaaga agtggcaaga aatatagcga atattaaaaa ggaattaaaa
      601 gaagtagagg aagaaatggc cagatattta gatgagttgg gattataggt tatttttgtt
      661 gccagggggg attttatatg agtagttcac taaacaaatc agacttctat tatggaacct
      721 ttttaactaa ggtcttagat aatggaaata aaccagccct agttagtaaa aatgatggca
      781 gaggaatata caaattaact accaataaag aggattatat tgtatatata aaatatgcta
      841 ctaataccaa taaaaaccat aaaagatgga cctttaacta tacagcaaac aatattgaag
      901 aaatcaaaaa atatatgaaa gaagataaaa atataatctt tgcttatgta tgtgcttata
      961 atgatttaag aaatagtgaa atagctatag caaacttgga agaattaaaa aaatgtataa
     1021 atccaaaatg taaaagaaat tcagttaata gagtatcaat atataaaaaa ccttatagtc
     1081 cagttttaag aatgtatgga acaaaaagag cggatataat gaatggaagg gataacacaa
     1141 tacatttaaa aagaaacagg ataaatgaac tataatggag gtgcaaattt gaaagaagag
     1201 ataagagaaa gaattgaaat gataaataaa ggccaagtac ctgaaggtta taaaaaaacc
     1261 aaggtaggaa tcataccaga ggattgggaa gagagttttt tagagaattt atgcgatata
     1321 tccactggag ataaagatac taaagatagg aaggaagatg ggaagtatcc attttttgtt
     1381 aggtcaaata atgttgaaag catagatagt tattcctatg acggtgaagc tattttaaca
     1441 gcaggggatg gagtgggcac tggtaaggtg ttccactatg tcaatggaaa gtttgattac
     1501 catcaaaggg tatattgttt aagagagttt agagatatta atggaaaata tttatattat
     1561 tattttagct ggaatttcat aaaacaagtc gtaaaatatt ctgctaaaac atcggtagat
     1621 tccgttagaa aagaaatgct gacaaagat
//
LOCUS       DATGSI010000357         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_354420, whole genome shotgun sequence.
ACCESSION   DATGSI010000357 DATGSI010000000
VERSION     DATGSI010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354420"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VK031_05555"
     CDS             complement(<1..662)
                     /locus_tag="VK031_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014092591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HLS53415.1"
                     /translation="MKNKRVTFLILSLLLFTPRIGYAMHIMEGFLPVKWAIMWGLLSI
                     PFIILGIRSINKNIKGDTNKKVLLGLAGGFVFVLSALKIPSVTGSCSHPTGVGLGAIL
                     FGPTTMTVIGLIVLLFQALLLAHGGLTTLGANTFSMAIVGPFVSYGIYKIIIRRKGNR
                     SVAVFLAAALGDLITYVVTSIQLSLAFPDLVGGFWTSLTKFLSVFAVTQIPLAIVEGI
                     ITV"
     gene            complement(652..1716)
                     /locus_tag="VK031_05560"
     CDS             complement(652..1716)
                     /locus_tag="VK031_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase"
                     /protein_id="HLS53416.1"
                     /translation="MLHGGDLLTYGRKYKGELLDFSTNINPLGVPKGLYEKLFDEFSK
                     LEIYPDIKYRKLKEAVSKYLGCDFENVLVGNGAVEIIDNFMIDAGRVIIVTPSFAEYE
                     LRAKVHKKEVISLPYKADFTVDTERIRQTIRREDLLVLGNPNNPTGLRIEKDTLLEIY
                     KICEEKAAYLLLDEAFYEFGPMDYDSIELFKADEYKNVGIIRAATKFFALPGIRLGYG
                     CASIDKVENISKVELPWSINALADKAGQLIFKDKEYIEKSRSYIDRERTLLLKELGKI
                     QGLHAYPTHTNYILIRLDSWDEEYVFNFFLERGILVRKCSRFENLPSNHIRVAIRSRS
                     MNERLLKVFKLLERGEKDEE"
     gene            complement(1718..2506)
                     /locus_tag="VK031_05565"
     CDS             complement(1718..2506)
                     /locus_tag="VK031_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS53417.1"
                     /translation="MIALETKDLTIKYGEKVALKNANIRIDKGEVVAVIGPNGSGKST
                     LIKGLSRCIKTSGGNIKLNNQDIRKIDSKLIAKEMAILPQLKDNHLDLEVETLVSFGR
                     YPHLGFGKRLQKKDRDIVSWALEITGLIQYRTTRLDSLSGGERQRAWIAMALAQKPKI
                     LLLDEPTTYLDISYQLEILELIKGLNEEMNLTVVMVLHDINQAARYAHKIYVLKDGEV
                     HDQGRPEEIVKDSLIKDVFNIDAKIYKDKINNCPYLVPLKLKEE"
     gene            complement(2503..>2770)
                     /locus_tag="VK031_05570"
     CDS             complement(2503..>2770)
                     /locus_tag="VK031_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HLS53418.1"
                     /translation="ILAASAVSVVGLLGFVGLIVPHMGRLLVGSDNRYLLPASVLLGG
                     STLMICDTLSRVLFAPMEIPVGIIMSATGAPFFLYLLNKKGVLK"
BASE COUNT          791 a          605 c          427 g          947 t
ORIGIN      
        1 accgttatta tgccttccac tattgctaag ggtatttggg tcactgcaaa aactgataaa
       61 aactttgtta aagaagtcca aaagcctcct acaagatctg gaaaggccag ggataattgg
      121 atggaggtaa ctacatatgt aattaagtcc cctaaggctg cagctaaaaa tacggcaact
      181 gacctattcc cctttcttct tataattatt ttataaatac cataggatac aaaggggcct
      241 actatggcca tggaaaaagt attagcacct agggttgtca gtccaccatg agccaataat
      301 aaagcctgga ataataggac tattaggcct attacagtca ttgtggtagg tccaaacaag
      361 atggctccca ggcctactcc tgtagggtgg gaacaggacc ctgtaactga tggaatcttt
      421 aaggctgata atacaaatac gaaacctcct gcaagaccca ataatacctt tttattggta
      481 tctcctttaa tgtttttatt gatacttcta atacctagaa taataaaggg tattgacaat
      541 aaaccccaca taattgccca cttaaccggt aaaaagcctt ccataatgtg catggcatag
      601 cctatccttg gtgtaaatag taataaggat agtattaaaa atgttactct tttattcttc
      661 atctttttct cccctttcta gtagtttaaa aaccttcaat agtctttcat tcatacttct
      721 acttcttatg gccaccctaa tgtggttgct gggtaagttt tcaaatctgc tacacttcct
      781 gactaaaatt cctctttcta agaaaaagtt aaatacatat tcttcatccc agctatctag
      841 cctgatcagg atataatttg tatgggttgg ataggcatgt agaccttgga ttttgcctaa
      901 ctcttttaat agaagggttc tctctctgtc tatgtaactt cttgattttt ctatatattc
      961 cttatcttta aatattagct ggcctgcctt atcagccaag gcattaatac tccatggaag
     1021 ctccaccttg ctgatatttt ctaccttgtc tatagaggca catccatatc ccagcctaat
     1081 gcctggtagg gcaaagaatt ttgtagctgc ccttatgatt ccaacatttt tatattcatc
     1141 tgccttaaat aattctatgc tgtcataatc cattggccca aattcataaa aggcttcatc
     1201 taatagtaaa tatgctgcct tttcctcaca tatcttatat atttctaata aggtatcttt
     1261 ttcaatccta agaccagttg gattgtttgg atttcccaaa actagcagat cctctctcct
     1321 tattgtttgc ctaatccttt cagtatctac agtaaagtct gccttataag ggagactaat
     1381 tacttccttt ttgtgtacct tggccctaag ctcatactca gcaaaggagg gggttacaat
     1441 gatcaccctc cctgcatcta tcataaaatt atcaattatt tcgacggccc cattacctac
     1501 caatacattt tcaaagtcgc agcccaggta tttgctaact gcttctttta atttcctata
     1561 cttaatgtct ggatatattt ctaattttga aaactcatca aatagctttt catacaggcc
     1621 ctttggaacg cctaaggggt taatatttgt actaaagtca agtaattcac ccttatattt
     1681 ccttccatag gtcagtagat ctcctccatg tagcatttta ttcctccttt aatttaagtg
     1741 ggaccaggta gggacaatta tttatcttat ccttgtatat cttggcgtct atattaaaga
     1801 catcttttat taggctgtct ttaactattt cctctggtct cccctggtca tggacctctc
     1861 catcctttag cacatagatc ttgtgtgcat atcttgctgc ctggtttata tcatgtaata
     1921 ccattaccac tgttagattc atttcctcat taaggccttt aattaattct aaaatttcca
     1981 actgatagga aatatctaaa tatgtagtag gctcatctaa taaaaggatc tttggtttct
     2041 gggccaatgc catggcaatc caagcccttt gcctttcccc tcctgacagg gaatctagcc
     2101 tggtagtcct atactggata agtccagtaa tttccagggc ccaacttact atatctctat
     2161 cttttttctg taatctcttt ccaaacccaa gatggggata tcttccaaat gatactagag
     2221 tttctacttc caaatctaaa tggttatcct ttaattgagg caatatggcc atttccttgg
     2281 ctatcagttt actatctatt tttcttatgt cttgattatt cagttttata tttccaccac
     2341 tagtttttat acatctactt agacccttaa tcagggtaga tttgccagaa ccattaggcc
     2401 caatgacagc taccacttcc cccttatcaa tccttatatt tgcattttta agtgcaactt
     2461 tttccccata tttaatggta agatccttgg tttctaatgc tatcatttta atactccttt
     2521 tttatttaat aggtatagga aaaaaggtgc ccctgttgca gacataataa tgcctacagg
     2581 aatttccata ggtgcaaaaa gaactctcga taaggtatca catatcatca aggttgagcc
     2641 acctaatagg acactggcag gtaataaata tctattgtca gaccctacta aaagccttcc
     2701 catatgaggc acaatcagtc ctacaaaacc taagagtcct acaacgctaa ctgcactggc
     2761 tgccaaaatt
//
LOCUS       DATGSI010000358         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_545600, whole genome shotgun sequence.
ACCESSION   DATGSI010000358 DATGSI010000000
VERSION     DATGSI010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545600"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..351
                     /locus_tag="VK031_05575"
     CDS             94..351
                     /locus_tag="VK031_05575"
                     /inference="COORDINATES: protein motif:HMM:NF020581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HLS53419.1"
                     /translation="MLKDLLRTIKKTKSYSANLVARKMNISESMVEDMMKQLIRMGYI
                     IEDKGVSHCETPCIKCPYMKTCNQNPATIYRISNKGEKLLH"
     gene            773..2209
                     /locus_tag="VK031_05580"
     CDS             773..2209
                     /locus_tag="VK031_05580"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HLS53420.1"
                     /translation="MNNIDWMNSRNLSMLADFYEFTMANGYLENNMEDEIAYFDMFFR
                     SIPDDGGFAIMAGVQQLIEYLNGDIEYFRSKGIFKEKFLNYLKDFEFTCDVWAIPEGT
                     PIFPMEPIVIVRGPVVQAQMIETMILLTINYQSLVATKANRMVRAAQGRPVIEFGSRR
                     AHSYEAAIYGARAAYIGGCIGTANTIVDRDYGIPALGTMAHSWVQMFPTELDAFRAYA
                     RVYPDNCYLLVDTYDTLRTGIPNAIKVFNEEIVPRGYRPKGIRIDSGDIAYLSKQARK
                     MLDEAGFPDCTIMASSSLDEYSIRNLINQNAAIDSFGVGERLITARSEPVFGGVYKLT
                     TVEKDGEIIPKIKISENVEKITTPGFKQVHRFFDRETNKAIADLVSLHHETVDDSKPY
                     EIFHPLFTWKRKTVRNFYARKLLVKIFDKGQQVYQSPSIHKIRDYCQDQIELLWPEVK
                     RFEKPHEYFVDLSQDLWDIKDKLLKENR"
BASE COUNT          741 a          340 c          468 g          706 t
ORIGIN      
        1 ccttttcagc catatatacc tttgttattg gctggataat ggcagtattg gtctatcaag
       61 ttggtttact attaggtttc taggagtgaa tacatgttaa aagatctgct taggaccatc
      121 aagaaaacaa aatcctattc tgcaaactta gttgccagga agatgaatat ttctgaatct
      181 atggtagaag atatgatgaa acaattgatt agaatgggat atataataga agataaggga
      241 gtttctcact gtgaaactcc ctgtattaag tgcccctata tgaaaacttg taatcagaat
      301 cctgctacta tttacaggat ttcaaataag ggagaaaaac ttttgcatta atttgataat
      361 ttacagaatt ttgtgattag aattaattat tagggtatat tatagataca accataaaaa
      421 tattaccctc atctaaatgg ttgtaagtaa aacttgattc tgttctggcc taaatttaag
      481 cattaccatt atgtttcaaa gatttaagtc tacagcaaaa tgtgtaagta tgtgttttaa
      541 actattgatt ctaaaaaaca tcagtaatat tgaccgaggg gtgtaagtta ttttaagaca
      601 aactgttaaa caatttttaa agggccagaa tagtaaaaac aagttttatt ctgattgata
      661 ggtgcttctt ttagatttta ttggaagcac ctatctaact tttttgtata tatttgtaat
      721 ttctccacta aaggtctata attgttatag gaatactgta aaggagataa agatgaataa
      781 tattgattgg atgaactcta gaaatctttc tatgttggct gacttttacg aatttaccat
      841 ggccaatggc tatttagaaa acaatatgga agatgaaatt gcatattttg atatgttttt
      901 tagatctata cctgatgatg gtggctttgc cattatggcc ggtgtacaac agttaataga
      961 atatttaaat ggggacattg agtactttag aagtaaaggc attttcaagg aaaagttctt
     1021 aaattactta aaagactttg aattcacctg tgacgtttgg gctattccag aaggtactcc
     1081 aatatttcct atggagccta ttgtaattgt aagaggccca gtggtgcagg cccaaatgat
     1141 agaaaccatg attttactta ctataaatta tcagtctcta gttgccacca aggccaatag
     1201 gatggttagg gcagcccaag gaaggcctgt tatagaattt ggtagccgta gggcccatag
     1261 ctatgaggca gctatttatg gagccagggc agcctatata ggtggttgta ttggtactgc
     1321 caatactatt gtagacaggg actatggtat ccctgcccta gggaccatgg cccatagctg
     1381 ggttcagatg tttcctactg aattagatgc ctttagagcc tatgccaggg tatatcctga
     1441 taactgttat cttttggtgg atacctacga taccttaagg acaggtatcc ctaatgctat
     1501 taaggtgttt aatgaagaaa tagttcccag aggttacagg cctaagggaa taaggattga
     1561 tagtggagat atagcctatt tatctaaaca ggccagaaag atgttagatg aggccggctt
     1621 tcccgactgt accataatgg cttctagcag tctagatgag tatagtataa gaaacctaat
     1681 taaccaaaat gcggctattg atagctttgg agtaggtgag aggttaatta ctgccagatc
     1741 tgagcctgta tttggcggag tttacaaatt aactacggtg gaaaaggatg gagagattat
     1801 acctaagata aagataagcg aaaatgtgga aaaaattact actccaggct ttaaacaggt
     1861 tcatagattc tttgataggg aaactaataa ggctatagca gatctggttt cactacatca
     1921 tgaaactgtt gatgatagca aaccttatga gatcttccac cccttattta catggaagag
     1981 aaagactgta agaaacttct acgccaggaa gttattagtt aaaatatttg ataaaggcca
     2041 acaggtttac caaagtccca gcattcataa aatcagagat tactgtcagg accagataga
     2101 gttactttgg cctgaagtaa aaaggtttga aaaacctcat gagtattttg tagacctatc
     2161 tcaggatttg tgggatatta aagataagct tctaaaagaa aaccgatagc aaatttgcta
     2221 tcggtttttt ggcggagaga gagggattcg aaccc
//
LOCUS       DATGSI010000359         1038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_168070, whole genome shotgun sequence.
ACCESSION   DATGSI010000359 DATGSI010000000
VERSION     DATGSI010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168070"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VK031_05585"
     CDS             <1..399
                     /locus_tag="VK031_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HLS53421.1"
                     /translation="ANSTEWDENTAQPVIDLMPEQKDLEAINGKMRLGLYPCKVMKDT
                     LAYRAYQEELIYERHRHKYEVNNEYVDRLVEKGLVISGISPDERLVEIIELKGHPWFM
                     GVNYHPEFKSRPTNAHPIFKEFIKAAKENK"
     gene            519..>1038
                     /locus_tag="VK031_05590"
     CDS             519..>1038
                     /locus_tag="VK031_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53422.1"
                     /translation="MKTMELGEFSRLYPIEEKIFIVPTRVIGSQMINKITREGYPTLN
                     LRAISLTRLAFEICQEYIIKKNKLIIDNILGSNLITQILKDSASQDENFFFQEELIDT
                     KTADEIYKVIMELKYAGLEKFPNIKDLDLIYEEYENRLEKLNAMDYHDVILKASQMER
                     LKEYREKRIGIAY"
BASE COUNT          412 a          147 c          209 g          270 t
ORIGIN      
        1 gccaatagta ctgaatggga tgaaaacacg gctcaacctg taatagattt aatgccagag
       61 caaaaagact tagaagccat taatggaaag atgaggctgg gtttatatcc ttgcaaggta
      121 atgaaggata ccttagccta tagggcctat caagaagaac ttatttatga aaggcacagg
      181 cataaatatg aagtaaacaa tgaatatgta gaccgattgg tggaaaaagg cttggtaata
      241 tcaggcatat ctcctgacga gcgtctagta gaaattatag aacttaaagg ccatccttgg
      301 tttatgggag taaattatca ccctgagttt aaatcaagac ctaccaatgc ccatcctatc
      361 tttaaagaat ttataaaagc agctaaagaa aataaataac ctaagcaata agcttcatat
      421 cagatagaga gggccaggct ctctcttttt ggtatataga aggactttta acctgaaaag
      481 tagaatatta taagaagtta ataaaaaggg gtggaaaaat gaaaactatg gaattagggg
      541 aatttagtag gctgtacccc atagaagaga aaatatttat agttccaacc agggttatag
      601 gttctcaaat gataaataaa atcacaagag agggttaccc aaccctaaat ttaagagcca
      661 taagccttac tcgcctggcc tttgaaatat gccaggaata tataataaaa aagaataaat
      721 taataattga caatatctta ggtagcaact taatcactca aatattaaag gattcggcca
      781 gtcaagatga aaatttcttt tttcaagagg aactaataga caccaaaaca gcagatgaga
      841 tctataaggt gattatggag ttaaagtatg caggattgga gaagtttccc aatattaaag
      901 atctagatct aatctatgaa gaatatgaaa ataggttaga aaaactaaat gccatggatt
      961 atcatgatgt aatactaaag gcaagtcaga tggaaaggtt aaaggaatat agagagaaaa
     1021 ggattggcat tgcatata
//
LOCUS       DATGSI010000360         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_71765, whole genome shotgun sequence.
ACCESSION   DATGSI010000360 DATGSI010000000
VERSION     DATGSI010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71765"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /gene="tuf"
                     /locus_tag="VK031_05595"
     CDS             <1..153
                     /gene="tuf"
                     /locus_tag="VK031_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269603.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HLS53423.1"
                     /translation="EEGVEMVMPGDNAKFTIELITPVAMEEGLRFAIREGGRTVGAGV
                     VSKVLE"
     gene            564..881
                     /gene="rpsJ"
                     /locus_tag="VK031_05600"
     CDS             564..881
                     /gene="rpsJ"
                     /locus_tag="VK031_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HLS53424.1"
                     /translation="MSKKDKQKIRIRLKAYDHELLDSSAERIVLAAKRTGADVSGPVP
                     LPTEREIITILRAVHKYKDAREQFEQRTHKRLIDILNPTQKTVDSLMKLNLPAGVDIE
                     IKL"
     gene            940..1569
                     /gene="rplC"
                     /locus_tag="VK031_05605"
     CDS             940..1569
                     /gene="rplC"
                     /locus_tag="VK031_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HLS53425.1"
                     /translation="MKNILGRKIGMTQIFTDNGSVVPVTVVEAGPVVVVQKKTVEKDG
                     YNAIQVGFEDAKENKINRPMKGHFDKAEVVYKRHLREFRVENPDDFEIGQEINVDIFQ
                     AGDKVDVIGTSKGKGTQGVIKRHGFSLGRATHGSTFHRSPGGLSASASPGRVFKGKRM
                     AGRMGNERVTVQNLEIVRVDADKNLLLIKGAIPGPKKGLVTIKETTIMK"
     gene            1597..2220
                     /gene="rplD"
                     /locus_tag="VK031_05610"
     CDS             1597..2220
                     /gene="rplD"
                     /locus_tag="VK031_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HLS53426.1"
                     /translation="MPKINVYNMSGDQVEEIELKEEIFDVEINQHVVYEVVKNQLANK
                     RQGTQSVKTRADVRGGGRKPWRQKGTGRARHGSIRSPIWVGGGVTFAPKPRDYSYRVP
                     KKVRRLAMKSALTSKVQNEEIIVVDELKFDAPKTKEMINVLENLNAEKKALIVTVNSD
                     MNIIRSARNIPNVEVTVVENLNVYDILKYDSFIITKEAVRKVEEVYA"
     gene            2220..2510
                     /gene="rplW"
                     /locus_tag="VK031_05615"
     CDS             2220..2510
                     /gene="rplW"
                     /locus_tag="VK031_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HLS53427.1"
                     /translation="MRIPHDIIIKPIITEKSMEEMADRKYTFMVDRRANKPEIKKAVE
                     EIFGVKVEKVNTMNMLGKVKRQGYTSGRRPSWKKAIVKLREDSDEIEFFEGM"
     gene            2539..>3116
                     /gene="rplB"
                     /locus_tag="VK031_05620"
     CDS             2539..>3116
                     /gene="rplB"
                     /locus_tag="VK031_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HLS53428.1"
                     /translation="MSIKKFKPTSPAIRQMTVSNFKEITKKEPEKSLLVSLNKSGGRN
                     SKGRITIRHRGGGAKRKYRIIDFKRDKDGVPGRVAAIEYDPNRTAYIALINYADGEKR
                     YILAPNKLKVGDTIESGEHADIKVGNALKLRNIPVGTTVHNVELHPGKGGQMARSAGA
                     SAQLVAKEGKYAQLRLPSGEFRLVSLECRATIG"
BASE COUNT         1206 a          413 c          723 g          774 t
ORIGIN      
        1 gaagaaggag tagaaatggt aatgccaggt gacaatgcta aatttaccat agagctaata
       61 acacctgtag ccatggaaga aggactaagg tttgctatcc gtgaaggtgg aaggacagta
      121 ggagctggag tagtatctaa ggttttagaa taataaagat atatttctat gaaatgaagg
      181 ggatcttctc cttcatttca tctgtataag aatatgtaaa ttttattaag aaaatagaaa
      241 taaaagtaga ttatttcagg aaaagccttg agttttttcc taatttaatg taaactatac
      301 tagtgtttat ttaaggcatt gcgatgatgc aaaaggtggt ccagcactca actgtttatg
      361 ataatatggg agagtgtgaa actctttgac tttataagat tgtctaaaga attgagtgct
      421 ggagaaattt ttgctgagaa tgtccggttt taaatcgggc gacttgaatt gctaaaatta
      481 aaaataaaaa aagaaacacc cggaagagtg tatcggcaat tccaagtcgc atgtaaaagc
      541 atgcgatagg aggagggaat taaatgtcaa agaaagacaa gcaaaaaata aggattaggt
      601 taaaggctta tgaccatgaa ttgttagatt catcagctga aagaatagtt ttagctgcta
      661 aaagaacagg ggcagatgtt tctggaccag taccactgcc tactgaaagg gaaattataa
      721 caatactaag ggcggttcat aagtataagg acgccaggga gcagtttgag caaaggacac
      781 acaaaaggct tatagatatc ctaaatccaa ctcaaaagac tgtagattca cttatgaagt
      841 tgaatctgcc agcaggtgtt gatatcgaga taaagttata attactttat atgattacag
      901 aaatgtaatc cgctgtaaaa caataggagg tgtaggaaga tgaagaacat tttaggtaga
      961 aaaataggca tgactcaaat tttcaccgat aatggttcag tggtaccagt tacagtagtt
     1021 gaagcaggac ctgtagtagt ggtacaaaag aagactgtgg aaaaagatgg ctacaatgct
     1081 attcaagtag gttttgagga tgccaaagaa aacaaaatta acaggcctat gaagggtcat
     1141 tttgataaag ctgaagtagt ttacaaaaga cacttaagag aatttagagt agaaaatcct
     1201 gatgatttcg aaattggaca agaaatcaat gtagacatat tccaagcagg tgacaaggta
     1261 gacgtgattg gaacttccaa aggtaaagga actcaaggtg taattaagag acatggtttt
     1321 agcttaggaa gggcaactca tggatccaca ttccacagat caccaggtgg actatcagcc
     1381 agtgcttctc cgggaagggt atttaaaggt aaaagaatgg ccggtagaat gggaaatgag
     1441 cgagtaacag ttcagaacct ggaaattgta agagtagatg cagataaaaa tttactactt
     1501 ataaaaggtg caataccagg gccgaaaaaa ggattagtaa ctataaaaga aacaaccatt
     1561 atgaaataac aattcaggct gaaaggagga agaaaaatgc ctaaaattaa tgtttacaat
     1621 atgtcaggag atcaggtaga agaaatagag ttaaaagaag agatctttga cgttgaaata
     1681 aaccaacatg ttgtatatga agttgtaaaa aatcaattag ctaataaaag acaaggaacc
     1741 caatcggtaa aaactagagc tgatgtaaga ggtggaggta ggaagccttg gaggcaaaaa
     1801 ggaaccggta gggcaagaca tggtagtatt agatcaccta tctgggtagg tggaggtgtt
     1861 acttttgctc ctaaacctag agattatagc tacagggtgc cgaaaaaggt aagaagatta
     1921 gccatgaaat cagccctaac ctcaaaggtt caaaatgaag aaattatagt agttgatgaa
     1981 ttaaaattcg atgcacctaa gactaaagaa atgattaatg ttttagaaaa tctaaatgcg
     2041 gagaaaaagg cattaatagt aacagtcaac agtgatatga atatcattag gtcagccaga
     2101 aacattccaa atgttgaggt tacagttgta gaaaacttaa atgtttatga catactaaaa
     2161 tatgattcat ttattatcac taaagaggct gtcagaaaag tggaggaggt gtatgcataa
     2221 tgcgtattcc acatgatatt attattaaac ctataataac tgagaaaagc atggaagaga
     2281 tggcagatag aaaatacact ttcatggttg atagaagagc aaataagcct gagattaaga
     2341 aggcggtaga ggaaatcttc ggagttaagg tggagaaggt aaatactatg aatatgcttg
     2401 gtaaagtaaa aagacaggga tatacttcag gtagaaggcc aagctggaaa aaagcaattg
     2461 taaaactaag agaagactca gatgaaatag aattcttcga aggaatgtaa taagtgttta
     2521 taagaaggag ggaaaacaat gagtattaag aaattcaaac caacttctcc tgctattaga
     2581 caaatgacag tatcaaactt taaagagata actaagaagg aaccagaaaa atccttacta
     2641 gttagcttaa ataaaagtgg gggaagaaac tctaaaggta gaataacaat ccgccataga
     2701 ggtggaggag ccaagagaaa atatagaata atagatttta aaagagataa ggacggagta
     2761 ccaggccgtg tagcagccat agaatatgat ccgaacagga ctgcctacat tgcattaatt
     2821 aactatgctg atggtgaaaa aagatatatt ctagcgccaa ataagctgaa ggttggcgat
     2881 acaatagagt ctggtgaaca tgcagatata aaggtaggga atgctttaaa attaagaaat
     2941 attccagtag gtacaactgt acacaatgtg gaattacacc ctggtaaagg tgggcagatg
     3001 gcaaggagtg ctggagcttc agcccagtta gtagccaagg aaggaaagta cgcccagctt
     3061 agactaccat caggtgaatt taggttagta agtttagaat gtcgtgcaac tattgg
//
LOCUS       DATGSI010000361         1602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_306495, whole genome shotgun sequence.
ACCESSION   DATGSI010000361 DATGSI010000000
VERSION     DATGSI010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306495"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VK031_05625"
     CDS             complement(<1..223)
                     /locus_tag="VK031_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HLS53429.1"
                     /translation="MTIGLNLENSFVGKEEIENIRPLVERSHHLLEEKTGAGSKYLGW
                     MDLPGQISKEELKRINKVAEKIKNNSDIFI"
     gene            complement(252..1391)
                     /locus_tag="VK031_05630"
     CDS             complement(252..1391)
                     /locus_tag="VK031_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HLS53430.1"
                     /translation="MLRKNKLLDRIHKSPDYSLLNYHVLEGMADWVRVIDRNGIVIYA
                     NTAMKEDLGEDIVGSKCYESICNVEKCDFCITNRSMVTGEIVQKEEVINGNYYSVKSS
                     PVRNGKGEIFAAVEVFRNVTRERKLELELINKNKKMNEDLQFAKRIQEKILPSKGRVG
                     NLRFDYVYKPSEMLSGDMFDIYRIDDKHIGIYISDVAGNGVAASMMTMFIHQMMRVMK
                     EETISPSKTLSNLHKRFLSLNLGAENYFTIFYAVYNKESKILKYANAGHNCIPIKFNG
                     GDIKLLENKGFPITMLLNNMEYEEKEVRLKNRDKILFYTDGVTEARDYSGKEFGLDNI
                     IRILKSNTNNILDTMEDEVINFSWGEHIDDLAIVLLEVLDEDLGN"
BASE COUNT          465 a          336 c          179 g          622 t
ORIGIN      
        1 ctataaaaat atctgaatta ttctttatct tttctgccac cttattaatc ctttttagct
       61 cttccttgga tatttggcca ggtaaatcca tccagcctaa atatttacta cctgcccctg
      121 ttttttcttc tagtaggtga tggcttcttt caacaagtgg cctaatattt tctatttctt
      181 ccttacctac aaaagaattt tcaagattta gccctatagt cataacatcc cctccatgtt
      241 tattaaagga cctagtttcc caggtcctca tctaatactt ccaataacac tatggccaga
      301 tcatctatat gctctcccca gctaaaatta ataacttcat cctccatagt atccaatata
      361 ttattagtat tgctttttaa aattcttatt atgttatcta agccaaattc cttgcccgaa
      421 taatctctag cttcagttac cccatctgta taaaaaagaa ttttatctct attttttaat
      481 cgcacttcct tttcttcata ctccatatta tttaataaca tggtaatagg aaagccctta
      541 ttttctaata atttaatatc tcccccatta aatttaatag gtatgcaatt atgtcctgca
      601 ttggcatatt ttaatatttt actttcctta ttataaacag cgtaaaagat cgtaaagtaa
      661 ttttcagcac ctaaatttaa ggataaaaac cttttatgca ggtttgataa agtctttgat
      721 gggctgatag tctcttcctt cataaccctc atcatttgat gaataaacat ggtcatcata
      781 gatgcagcca ccccattgcc agccacatcg cttatatata tgccaatatg cttatcatct
      841 atcctataga tatcaaacat atccccactt aacatttcag agggcttata cacataatca
      901 aatcttaggt ttcctaccct tcctttactg ggaagaattt tttcctgtat tcgttttgca
      961 aattgtaggt cttcattcat cttcttgttc ttattaatta attctagttc tagttttctt
     1021 tctctagtta catttctaaa gacttctact gcagcaaaaa tctctccttt gccatttcta
     1081 actggtgaac ttttaacaga gtagtaattt ccgttaatca cttcctcttt ttgtacaatt
     1141 tcaccagtta ccatactcct attagtaatg caaaagtcac atttttccac attgcaaata
     1201 gactcataac atttacttcc tactatgtct tcacctagat cctctttcat agctgtatta
     1261 gcatatatta caattccgtt tctatctatg actctaaccc agtctgccat accttccagc
     1321 acatgataat ttaacaggct ataatctgga gacttatgga tcctatctaa aagcttattt
     1381 ttccttagca ttatatcacc taactctatt ttttaatcac tgtataatgc aaatttaaaa
     1441 atatgttgtt ttatgtcaat taagtgtagt tattgtatat tctattctat agtaaatcgg
     1501 gcctaatagc cagaagtttt cttacttata ttgtaccact agattgtaat gttaagtgac
     1561 gaagtttttc aagaaatatt ctatacgttt gttttgctta ga
//
LOCUS       DATGSI010000362         1768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_403354, whole genome shotgun sequence.
ACCESSION   DATGSI010000362 DATGSI010000000
VERSION     DATGSI010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403354"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VK031_05635"
     CDS             <1..1357
                     /locus_tag="VK031_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53431.1"
                     /translation="GHVENSQNTFLNQALNTLSNYGCRGLVNLKNNWIVNIENRALDA
                     SSNIKTKDARENLDISDIWITDPPYADAVNYHELTEFFLAWDKSLVEKAFPEWYTDSR
                     RILAVQGVGENFNNSMIEIYSNLNKHMPNNGTQVVMFTHQDVKVWAELAMILWSAGLR
                     VVSAWTISTETASGGLKSGNYVSGTVILTLKKQLTEEKIYQDELYDEIRHEVESTIDT
                     MKDINDKDDPDFNDADFILASYAAALKAITSFNEIAGIDIPYWLEQDRNSVKKNPIEE
                     LISKAQDIAYNYLIPEGFDPFIWRELSVEERFYIRGLELEMNNIYQVGDYQELARGFG
                     VTDYTNMFNSTSANKARFKTAEEFKRRSMGEDGFGNTLTRHLLVAISDSITEKKASAG
                     VVYLKATFQANNEYWSKKNLMYEILSFIAKVENIDHMEHWKDTAYYAKVLREALRNDT
                     V"
     gene            1375..>1768
                     /locus_tag="VK031_05640"
     CDS             1375..>1768
                     /locus_tag="VK031_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53432.1"
                     /translation="MINRYSSRRESLSDSLFNRKLKNAQSYDRIAGYFSSSILEIAGE
                     EIESIKGKVRVICNSELDARDVATAKSAALGIRKEWCDFKPENLKNSSKRFNRLYKLL
                     SSGKMEVRVLPNDKFGLMHGKAGIITSKD"
BASE COUNT          650 a          235 c          377 g          506 t
ORIGIN      
        1 tggacatgtt gaaaattcac aaaacacctt tttaaaccag gcattaaaca cattatctaa
       61 ctatggttgt agaggattag ttaatcttaa gaataattgg attgttaata ttgaaaacag
      121 agcattggat gctagtagca atattaagac aaaggatgcc cgtgaaaatt tagatatatc
      181 tgatatatgg attactgacc ccccttatgc agatgcggtt aactatcatg aattaacaga
      241 gtttttctta gcgtgggata aatcattggt ggaaaaggcc ttcccagagt ggtatacaga
      301 ttctagacgt atattagctg tgcaaggtgt tggagaaaac tttaataata gtatgattga
      361 aatatacagt aatttaaaca agcatatgcc caacaacgga acgcaggtag tcatgtttac
      421 acatcaagat gtaaaggttt gggccgaact agctatgatt ttatggtcgg ctgggcttag
      481 agtggtttca gcttggacta tatcaacaga gactgctagc ggtggtttaa agagtggtaa
      541 ttatgtctca ggaactgtaa ttttaactct taaaaagcag ttgacggagg aaaagatata
      601 tcaagatgaa ctatatgatg aaattagaca tgaggttgag agcacaatcg atactatgaa
      661 agatataaat gataaagatg acccagactt taatgacgca gattttatat tagcatctta
      721 tgctgcagca ttaaaagcta taacatcttt caatgaaata gcaggaatag atattcctta
      781 ttggttagaa caagatagaa atagtgtgaa aaaaaaccct attgaagaac tcatatctaa
      841 agctcaagat atagcttata attatttgat acctgaaggt tttgatccat ttatatggag
      901 agagttatct gtagaagaaa ggttttatat aaggggtcta gaactggaaa tgaacaatat
      961 ttatcaagta ggagattacc aggagttagc aagaggattt ggagttacag attacacaaa
     1021 catgttcaat tctacctcgg caaataaggc tagatttaaa acagctgaag aatttaagag
     1081 aagatctatg ggagaagatg ggtttggaaa tacattaacc agacatctat tagtagctat
     1141 cagtgatagc ataacagaaa agaaagcttc ggcaggtgtc gtatacttaa aggcaacatt
     1201 tcaagcgaac aacgaatatt ggagtaaaaa gaacttaatg tatgaaatcc ttagctttat
     1261 agcaaaggta gaaaacatag accatatgga gcattggaag gatacggcat attatgccaa
     1321 ggtattaaga gaggctctta gaaatgatac tgtgtaagta taggagtgat ctttatgata
     1381 aataggtatt catcaagaag agagtcttta tcagactctc tttttaatag aaaattaaaa
     1441 aacgctcaat cttatgatcg aattgcaggt tacttcagtt cttcaatttt agaaatagcg
     1501 ggagaagaga tagagagtat aaaaggtaaa gttagagtga tttgcaattc tgaacttgat
     1561 gccagggatg tagcaactgc aaaatctgct gcactcggga tcagaaagga atggtgtgac
     1621 tttaagccag aaaatctaaa aaactcatct aaaagattta atagactgta taaacttctc
     1681 tcgtctggga aaatggaagt tagagtgttg cctaatgata aatttggtct aatgcatggt
     1741 aaagcgggaa taattacatc aaaggatg
//
LOCUS       DATGSI010000363         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_448811, whole genome shotgun sequence.
ACCESSION   DATGSI010000363 DATGSI010000000
VERSION     DATGSI010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448811"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="VK031_05645"
     CDS             <1..1162
                     /locus_tag="VK031_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HLS53433.1"
                     /translation="WQGGQKALVVLRIFLLIGLVTASWLSAGTIPAIVYYSVKVMKAE
                     LFLVFAFLISSLVSYMLGTSFGTASTIGLVLIIMARSGKINLNLVGGAILSGCYFGDR
                     ASPMSSSANLISNLTEAELYSMLKNFLKTTILPFILTSLIYLFLSLSNPLKLSDSQIV
                     IDLQNNFTINYFILIPALAMLVLSLFQIRVRSSMLVSIVLGSIISILVQGNSLPNLTK
                     TLIFGYRLKANNPLANILKGGGLISMLQPAFIVFVSCSMTGILEAIGLFEMVDKIFNN
                     IKKRWQLFSATALVSFFAAAFGGNQSIAVVMTSQIMKDIYERHKVDKYGLATDISNTT
                     ILFAPMIPWNIANLVPATTLSVNPAKIVPYAFYLYLPFLINWLYFWQGERNS"
     gene            1216..>1695
                     /locus_tag="VK031_05650"
     CDS             1216..>1695
                     /locus_tag="VK031_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLS53434.1"
                     /translation="MTNFYQVKEFCPSGSFPYAIKAGDTLYNLAQEFNTSVEAIMELN
                     PRLNPYNLQTGQIICIPAETPAKCPANSFEYTIKSGDTLYNLAKRYDTSVEEILAINP
                     NLNPYNLQVGQKICIPSKAPAKCPANSFEYTIKAGDTLYNLANQYNTSVEEILAINPS
                     "
BASE COUNT          529 a          345 c          301 g          520 t
ORIGIN      
        1 ctggcaggga ggtcaaaaag ccctggtggt tttaaggata tttctactaa ttggcttagt
       61 taccgccagc tggttgtcag ctggaactat tccagccata gtttattaca gtgtaaaggt
      121 tatgaaggca gaattatttc tagtatttgc ctttctaata agttctctag tttcctatat
      181 gctaggtacc tcctttggaa ctgccagcac cataggcctg gttctgatta tcatggctcg
      241 aagtggcaag atcaacctta atctggttgg aggagctatt ttgtctggct gctattttgg
      301 agacagggcc tctcccatgt cttcttccgc caacctaata tcaaacctaa cagaagctga
      361 actgtattca atgttaaaga attttttaaa gactaccatc ctgcctttta tcctaactag
      421 cctaatatat ttattccttt ccctttcaaa tcctttaaag ttatcagaca gtcaaatagt
      481 aatagatcta caaaataatt tcaccattaa ctactttata ctaattcctg cccttgccat
      541 gctagtctta agcctttttc aaataagggt aagaagttct atgttggtaa gcatagtatt
      601 aggctctata atatccatat tagttcaagg aaactcttta cctaacttaa ccaaaactct
      661 aatctttggc tataggttaa aggccaataa tcctctagcc aatatattaa aaggtggagg
      721 acttatatct atgcttcagc cggcctttat agtttttgta tcctgttcta tgacagggat
      781 tttggaagcc ataggtctct ttgaaatggt agataaaatt ttcaataata taaaaaaaag
      841 gtggcagctt ttctcggcca cagccctagt atcatttttt gctgcagcct ttggtggcaa
      901 tcagtcaata gcagttgtga tgaccagcca gattatgaag gacatatatg aaagacataa
      961 ggtggacaaa tatggtttag ccactgatat atctaatact actattttat ttgcacctat
     1021 gattccctgg aatattgcca acctggtgcc ggccaccacc ctatcagtca atccagccaa
     1081 gatagttccc tatgcctttt acctttacct accttttcta ataaattggc tatacttttg
     1141 gcaaggggaa aggaactctt aaccagcttt tgcataatat ataatgcaaa gactaatgtt
     1201 agaaaggtgg ttaagatgac taatttttat caagtaaagg aattttgtcc cagtgggagc
     1261 tttccctatg ccataaaggc aggagatacc ctttataatc tagcccaaga atttaataca
     1321 agtgtagagg ccataatgga gctaaatcca aggttgaatc cctacaatct tcaaacaggc
     1381 caaataattt gcataccagc agaaacaccg gccaaatgtc cggctaattc ttttgagtat
     1441 acaataaaat ctggagacac cctatataat ctggctaaga gatatgacac aagtgtagaa
     1501 gagattttgg ctataaatcc aaatctgaac ccttataatc ttcaagtagg ccaaaaaatt
     1561 tgcataccta gtaaggcacc ggccaagtgt ccagccaact cctttgaata cacaataaaa
     1621 gcaggagata ccctttacaa tttagccaac caatataata caagcgtaga ggagatattg
     1681 gccataaatc caagc
//
LOCUS       DATGSI010000364         1077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_448904, whole genome shotgun sequence.
ACCESSION   DATGSI010000364 DATGSI010000000
VERSION     DATGSI010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448904"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VK031_05655"
     CDS             <1..470
                     /locus_tag="VK031_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLS53435.1"
                     /translation="VVTINHFSPGNVFGEALVFSDQHLYPATVISSEPAKILYIQRQD
                     IIRLMSYDERIIDNFMRVLSNRILMLNDRITNLSYDTLRKKIANALLFQYNKQKSKTI
                     ILPYSRKKMAELLNIPRPSLSRELIKMQEEGIITFHRNKVEILDLKSLEDSLI"
     gene            complement(510..>1077)
                     /locus_tag="VK031_05660"
     CDS             complement(510..>1077)
                     /locus_tag="VK031_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HLS53436.1"
                     /translation="YSISATFIFDILMVILFPIAGRYFGMSDLGFGLWTGTAVNDTSS
                     VVAAGYAFSHAAGNFALIVKLTRTLSIIPTVLAFSYINERINAKQNGNKEDKVKVDIG
                     KIFPYFIILFLLMVGIKSTGLISDGLSNQISSVSKFAMIMSLGAIGLKTNFKEVSKSG
                     FLPMLHGFIISALVVVVSFMVQMMMGQI"
BASE COUNT          372 a          194 c          182 g          329 t
ORIGIN      
        1 aagtggttac tatcaatcat ttctcccctg gtaatgtatt cggcgaggct ttagtttttt
       61 ctgaccagca tttatatcca gccacagtta tttcctctga accggccaag attctgtaca
      121 ttcaaagaca ggatataata aggttgatga gctatgatga aagaattatt gataacttta
      181 tgagggttct atctaatagg atccttatgt taaatgatag gattacaaat ttatcttatg
      241 ataccttaag aaaaaaaata gccaatgcct tattattcca atataataag caaaaatcta
      301 aaactattat cctgccttat agtaggaaaa agatggctga gcttttaaat attccaagac
      361 cctccttgtc tagggaatta atcaagatgc aagaggaggg tattattact ttccatagaa
      421 ataaggttga aattttagat ttaaaaagtc tggaagactc attgatataa aggaaaaagg
      481 ccgaaagttc tcggcctttt taatcctaat taaatctgtc ccatcatcat ttgaaccata
      541 aaagatacta ccaccactag ggctgaaatt ataaaaccat gtaacatagg tagaaatcct
      601 gatttagata cttccttgaa attagtttta agccctatgg ctcccaggga cataatcatg
      661 gcaaacttac taacggaaga aatctggttg cttagcccat ctgatataag tcctgtactc
      721 ttaatcccca ccataagcaa aaataaaata ataaaatagg ggaatatctt tcctatatct
      781 accttaacct tatcttcctt gttgccattt tgcttggcat ttatcctttc atttatataa
      841 gaaaaggcca aaactgtagg aataatagat agggttctag ttaattttac tattagggca
      901 aaattgcctg cagcatggga aaaggcatag cctgctgcta ccactgaaga ggtgtcattt
      961 actgcagtac ctgtccaaag accaaaacct aagtcgctca tgccaaagta cctgcctgct
     1021 attggaaata atatgaccat taatatatca aatataaagg tggcagaaat agagtag
//
LOCUS       DATGSI010000365         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_354734, whole genome shotgun sequence.
ACCESSION   DATGSI010000365 DATGSI010000000
VERSION     DATGSI010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354734"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VK031_05665"
     CDS             <1..577
                     /locus_tag="VK031_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HLS53437.1"
                     /translation="ILDKFSVNKGENIFKVRLSDGRKDIGEIPYIKKVNIKRNLPDTI
                     SINIEERKEIALIKNLSMYLLVDEEGYILDYVDRPKEDLPVIKGQGIKDVKLGENIFL
                     KDTEDMTEFVTRTKEMGILEKARLLELDENGEINISLKNGIDIAFGSIDNVKYKLELL
                     NEILDDIKKKNINCKKIIMNKGNHPVLELDD"
     gene            592..1311
                     /locus_tag="VK031_05670"
     CDS             592..1311
                     /locus_tag="VK031_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HLS53438.1"
                     /translation="MKKSKELLALTLVSIFLGIILAIQFKTVNRTVGEGVLPTQRAQQ
                     LAAELKKVQAERDSYLNRANELEEKIEQYEKGEVDQDAYAENLYKDTLKYRMLAGYMD
                     LEGEGIILEINDPPEEVQVGGGFTIVDELDLILQIISILNAADAEAISINDQRYTTYT
                     EIVRAGNHIEINGVSTNAPIVIKAIGNPDTLESALMFKGGVVWELRRYNYLVHLTQDK
                     NIQIPKYRMVKEFKYAVPVEG"
     gene            1336..2061
                     /locus_tag="VK031_05675"
     CDS             1336..2061
                     /locus_tag="VK031_05675"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HLS53439.1"
                     /translation="MYKYNKKVLFLTSVLVGIMISILMKANVEMYAPVTLRSIQNIRD
                     EINFVSNELVQLKELVEQREEELEVIENISKSGQNIIDLLAEDYRYNKVKSGYTALEG
                     PGIVITMFDNPEEAIVGFDINDDIIHDVDILNILNDLRVAEAEAISINGERVLASSEI
                     KCRGPVVRINGTSFGAPFVIKAIGDPKVLMASVNAPGTYGETLKTVYGIGFETKVEDK
                     IYIPAYSKALNFRYAKPKGEGDV"
     gene            2061..2402
                     /locus_tag="VK031_05680"
     CDS             2061..2402
                     /locus_tag="VK031_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HLS53440.1"
                     /translation="MFFALIGVLIGGVIGFLLPYTYNTAYSLYITVAILACLDSVFGG
                     IRANLEKSFNMKVFLSGFFVNSILAAFLAYIGDRLGVPLYYAAIFTFGGRMFDNFASI
                     RRLLLFKNKSM"
BASE COUNT          902 a          302 c          578 g          778 t
ORIGIN      
        1 tattttggat aaatttagtg ttaataaggg tgaaaatata tttaaggtaa ggctttctga
       61 tggaaggaaa gatattgggg aaattcctta tataaaaaag gtaaatatta aaaggaactt
      121 gcctgatact atttcaatta atatagagga aagaaaagaa atagccctta ttaaaaacct
      181 atctatgtat ttacttgtgg atgaagaggg atatatttta gactatgtag ataggccaaa
      241 ggaagattta cctgttatta agggacaggg aattaaggat gtaaagctag gtgaaaatat
      301 attcttaaaa gatacagagg atatgacgga atttgttaca aggacaaaag aaatgggtat
      361 attggagaaa gcaaggctat tagaacttga tgaaaacgga gaaattaata ttagtttaaa
      421 aaatggcatt gatattgcat ttggtagcat agataatgta aaatataagc tagagctatt
      481 aaatgaaatt ttagatgata taaagaaaaa gaatattaat tgcaaaaaaa ttattatgaa
      541 taaggggaat catcctgtat tagaacttga tgattaagag agagtgatga gatgaagaaa
      601 tctaaggaat tattggcatt aactttagta tctattttcc taggtataat acttgctatt
      661 caatttaaaa ctgtaaacag gactgtggga gagggggttt taccgaccca aagagcccag
      721 caattggcag ctgaattaaa aaaggttcag gctgaaaggg attcttatct caatagagct
      781 aatgaattag aggaaaaaat agaacaatat gaaaaaggag aggtagacca ggatgcctac
      841 gcagaaaacc tatacaagga tactttaaaa tataggatgt tggcaggtta catggactta
      901 gaaggggaag gtattatcct ggagataaat gatccaccag aggaggtcca ggtaggcgga
      961 ggctttacca ttgtagatga gctggattta atattacaaa taattagtat cttaaatgca
     1021 gcagatgctg aggctatttc tattaatgat caaagatata ccacatatac agaaattgta
     1081 cgagctggta accatattga gataaatgga gtctctacca atgcacctat agttattaag
     1141 gccattggta atcctgatac tttagagtca gccttaatgt ttaagggtgg agtagtctgg
     1201 gaattaagaa ggtataatta cttagttcat ctaactcaag ataaaaacat acagattcct
     1261 aaatatagga tggtaaagga atttaaatat gcggtgccag ttgaaggcta accaatagat
     1321 atgtgagtga tatttatgta caaatataac aaaaaggtat tatttctgac aagtgtttta
     1381 gtcggtatta tgatttctat attaatgaag gccaatgtgg aaatgtatgc acctgttact
     1441 ttaagatcca tccaaaatat tagggatgag ataaattttg ttagcaatga gttagtccaa
     1501 ttaaaggaat tggtggaaca aagggaagag gaactagaag ttattgaaaa catctccaaa
     1561 agtggacaaa atataataga ccttttggcg gaggattaca gatataataa ggttaagtca
     1621 ggttatactg ccttggaggg gccaggaatt gttataacca tgtttgataa tccagaggag
     1681 gccatagttg gctttgatat aaatgatgat attattcatg atgtggatat attaaatatt
     1741 ttaaatgatt taagagttgc agaagctgaa gccattagca taaatggtga aagggtgctg
     1801 gctagttctg aaattaagtg tagggggcca gtagttagaa ttaatggtac cagttttggc
     1861 gccccttttg taattaaggc cataggggat cctaaggttt taatggcctc tgttaatgca
     1921 ccaggaacat atggtgagac tttaaagact gtttatggca ttggttttga gactaaggta
     1981 gaagataaga tatatatacc tgcttactcg aaagcgttaa attttagata tgcaaagcct
     2041 aagggagaag gtgatgttta atgttctttg cgctaatcgg agtcttgata ggaggggtaa
     2101 ttggattttt actaccttat acctataata ctgcctattc actgtacata acagtagcca
     2161 tattggcttg tttagactct gttttcggtg gaatcagggc caatttggaa aagtccttta
     2221 atatgaaggt ctttctgtct ggtttttttg tcaactccat actggccgct tttttagcct
     2281 acatagggga taggcttggc gtacctctat attatgcggc tatttttaca tttggaggta
     2341 gaatgtttga taactttgct tcaatcaggc gattattatt atttaaaaat aaatcgatgt
     2401 agatatgtta actacaattg aaaaaaatat ttcagcaact attagacgag ggggaaggta
     2461 gaatgtttga tattgacatt gatgttgaac aatttgcaag gattaaggta attggcgtag
     2521 gcggtggagg gagcaacgct gtaaacagga tgatagagca
//
LOCUS       DATGSI010000366         3445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_306868, whole genome shotgun sequence.
ACCESSION   DATGSI010000366 DATGSI010000000
VERSION     DATGSI010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306868"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VK031_05685"
     CDS             complement(<1..290)
                     /locus_tag="VK031_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS53441.1"
                     /translation="MMIKIENLHKYFGKKHVLKNINLEIKRGEVVGLIGPSGSGKSTL
                     IRTMNHMEEPSSGKVYFLGKDLMKSQDTIDEIRQKIGMVFQHFHLFPHKTVL"
     gene            complement(280..945)
                     /locus_tag="VK031_05690"
     CDS             complement(280..945)
                     /locus_tag="VK031_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HLS53442.1"
                     /translation="MNDLVIIELFTKYSYYYLAGIKNTILLSFLSLIIGSILGCILAL
                     LRLSDVKIFRFISTAYIEIIRGTPILVQISMVYFGSYVLFGIDINKFLSALIAVALNS
                     AGYVAEIIRSGIQSIDKGQMEASRSLGVSHRHTMQYIILPQAIKNILPALGNEFVTLI
                     KETSVASVIGTADLMYASKIVSSKSYKPFSPLVVIAIIYFLLTFSLSQLVAYFERRMA
                     HDD"
     gene            complement(978..1799)
                     /locus_tag="VK031_05695"
     CDS             complement(978..1799)
                     /locus_tag="VK031_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HLS53443.1"
                     /translation="MFKGKGKKVNLLIILLLITSLILTACGGKTDGNGEKTVEDIKEA
                     GKIVLGTSADYPPFEFHALIDGKDEIVGFDIELANYIAEKLGVELEISDMDFDSLITA
                     LGAGRVDMVIAGMNPDPEREANFTDIYYEANLAVLVMEDNPHGIKIEEDLNGKRIGVQ
                     IGTTQEDVASGLDAEEVLSLASNYDLVMNLKSNKIDCAIMELPVAQSFAKNNDDLIVV
                     EGLEIDSGTDGLAIAVQKGNDQLTDFLNEIIAEVKEKGLIEEWMAKADELSSEEI"
     misc_binding    complement(1968..2216)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2387..>3445
                     /locus_tag="VK031_05700"
     CDS             2387..>3445
                     /locus_tag="VK031_05700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLS53444.1"
                     /translation="MDLKEFKGLPKSFWIASTGRTNYPKLEEDIEVDLAIVGGGMVGI
                     STAYQLRESGLDIGILDAGGILMSTTAHTTAKITSQHGLIYQKIKSKFNKDMAEGYAK
                     ANEFAIELVEKIITKHKIDCDFERQAAYIYTQEDKYIKKIEKETDLAISLGLPASYLD
                     DIPLNIPIKAAMRFDDQAQFHPRKFLLALADIIHQDGVKIYEHSRVIGLEEDGESFIL
                     LTSQGKRVKANKVIIASHYPFYNKESIYFARLYVERSYVLALKANEKYPGGMYISAED
                     PVRSLRAQDSNEGQLIFVIGEKHKTGQGEDTNIHYENLIDFAQGLFTIEDIPYRWSTQ
                     DCMTMDNLPYIGQYSKDYP"
BASE COUNT         1027 a          703 c          582 g         1133 t
ORIGIN      
        1 tctaagactg ttttatgggg aaatagatga aagtgttgga agaccatgcc gattttttgt
       61 cttatttcat ctattgtatc ctgcgatttc atcaggtcct tgcctaagaa atataccttg
      121 ccagaactag gctcttccat atggttcatg gtcctgatta aggtgctttt tccagagcct
      181 gagggcccta taagccctac cacctctccc ctttttattt ctaaatttat attttttagc
      241 acatgctttt tgccaaaata cttgtgtagg ttttctatct taatcatcat gtgccattct
      301 cctttcaaag tatgctacca attgggataa tgaaaatgtt aaaagaaaat atattatggc
      361 tataacaacc aaggggctaa agggtttgta acttttactg gatactatct ttgatgcata
      421 cattaagtct gcagtcccta ttactgaggc cacagaagtc tccttaatca aggttacaaa
      481 ttcatttccc agggctggta aaatattttt tatggcttga ggtaaaatga tatattgcat
      541 ggtgtgcctg tgggaaactc ccaaactcct actggcctcc atctgcccct tgtctataga
      601 ctggatacca gatcttatga tttctgccac ataaccagcg ctgtttaagg ctacagcaat
      661 taaagccgat agaaatttat ttatgtctat gccgaataat acataggatc cgaagtaaac
      721 catggatatt tgaaccaaga taggggttcc cctgattatt tctatatagg cggtggaaat
      781 aaatctaaat attttcacat ctgatagcct taacagggcc aggatgcagc ctaatatact
      841 tcctataatc aaagataaaa atgataacag tattgtattt tttattccag ctagatagta
      901 atagctatat tttgtaaata attctataat taccaagtca ttcactccta aatctgctct
      961 ttatttccac cacttcttta tatctcttca ctgcttagtt catctgcctt ggccatccat
     1021 tcttctatta ggcccttttc tttaacttca gctataattt catttaaaaa atcagttaat
     1081 tggtcattac ccttttgaac tgctatggct aatccatctg tgcctgagtc tatttctaag
     1141 ccttccacca caataagatc atcattgttt ttggcaaagg attgggccac cggtaattcc
     1201 attatggcgc aatctatctt gttgcttttt agattcataa ccagatcata gttggatgcc
     1261 aaacttaaaa cttcctctgc atccagccca ctggccacat cttcctgggt ggtgcctatt
     1321 tgtacaccta tccttttacc atttaagtct tcttctattt tgatcccatg aggattatcc
     1381 tccattacca aaacagctag attggcctca taataaatat ctgtaaagtt ggcctctctt
     1441 tcaggatctg gattcatacc agcaattacc atatctaccc tacctgcacc cagagcagta
     1501 atcaagctat caaagtccat atcacttatc tccaactcta cccctagctt ttctgctata
     1561 tagttggcca gttcaatatc aaagcctact atttcatctt ttccatctat tagggcatgg
     1621 aattcaaagg gtggatagtc tgcactggtt cctaaaacta tttttccagc ttccttaata
     1681 tcctccactg ttttttcacc atttccatcg gtcttgcctc cacaggctgt taagattaaa
     1741 ctagttatta ataaaagaat tattaataga tttacttttt tacccttacc tttaaacatt
     1801 ttatttcctc cctttattta attaacaggt aataaattac taatgccact atacttggtg
     1861 ctccaagtag taaaaaactt ttcatctccc ccctttcttt ttttcctgtt agcctgtttc
     1921 aaggccttga aaatctccat taaaaaaact ctcatcccta gcctatagct aggaacgaga
     1981 gttatatccg cgttgccatc ctaatttacc tatgcttcac agcataggcc tttataggta
     2041 catcaaactt gattataccc tgtcactata acgggtgatc ccgttgcagc cttgattaca
     2101 aatctcggtg caaaactcag aggccctctt cactttaata gaccttgttc cctttcacca
     2161 aacggaactc gctgtaaaga tattcttaaa gctactctcc tcgtcattgt ccttaatcct
     2221 tctttgaaat tatcagttat tatacaataa aaaaatataa atgtaaagcc tttttaaaga
     2281 aaaattttta tcctgctaaa ttgctagctt gtgaaagtta aattgttagt ataaatatgc
     2341 agtatagaca gaaaatatga atataaaata aatactggag gttaatatgg atcttaaaga
     2401 atttaagggt ttacctaagt cgttttggat tgcatctact ggtagaacta attatcctaa
     2461 attagaggaa gatatagagg tggatctggc catagtaggc ggaggcatgg ttggtataag
     2521 tacagcctac cagcttagag aaagtggact ggatattggg attttagatg ctgggggcat
     2581 acttatgtct accacagccc atactacagc caagattaca tcccaacatg gcctgattta
     2641 tcaaaaaatc aaatctaaat ttaataagga tatggctgaa ggctatgcaa aggccaatga
     2701 atttgccata gaactggttg agaaaattat tactaagcac aaaatagact gtgattttga
     2761 aaggcaggct gcatatattt atacccagga ggataaatat attaagaaaa tagaaaagga
     2821 aacagacctt gccatatccc ttggcctacc tgcctcctat ttagatgata tccctcttaa
     2881 tatacctatt aaggctgcca tgagatttga tgatcaggcc cagtttcatc ctagaaaatt
     2941 tttactggcc ctggcagata ttatccatca ggatggggtt aagatttatg agcatagtag
     3001 ggttattggg ctagaggagg atggagaaag ttttattttg ttaacttccc agggtaaaag
     3061 ggttaaggca aacaaggtaa taatagcctc tcattaccct ttttataata aggaatctat
     3121 ctactttgcc aggctttatg tggaaaggtc ctatgtcctg gccctgaagg ctaatgaaaa
     3181 atatccaggt ggcatgtata ttagtgctga agaccctgtc cgctccctaa gagcccagga
     3241 tagtaatgag ggccagctta tctttgtaat tggtgagaaa cataagacag gtcaagggga
     3301 ggatacaaat attcattatg aaaatttaat tgactttgct caaggccttt tcaccataga
     3361 agatatcccc tatagatggt caacccagga ttgtatgacc atggataatc ttccctatat
     3421 tggtcaatat agtaaagact atcca
//
LOCUS       DATGSI010000367         1321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_122464, whole genome shotgun sequence.
ACCESSION   DATGSI010000367 DATGSI010000000
VERSION     DATGSI010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122464"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1134)
                     /gene="hrcA"
                     /locus_tag="VK031_05705"
     CDS             complement(94..1134)
                     /gene="hrcA"
                     /locus_tag="VK031_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HLS53445.1"
                     /translation="MLDDRKLKVLLAIINSYISSAEPIGSRTITKKYDLGVSSATIRN
                     EMSDLEDLGYLNKPHSSAGRVPSDKAYRFYVDELLKSNKIATDIIKKLKIKEALMKES
                     WEIDELIQNSAKILSALTSHTAVVASPRMKNSKIRQIQLVDLDEKEVLLVIVCETGTV
                     KNSILKLDKPVTSDQLTIISNFLNSKLSGLTIDQLSNQLDENVFKEMYEFKSILEKLI
                     PILNTSIDDLVSVDLYADGLTKILNFPEYKDVEKAKDFISFVEDKKLMLEILSQSPTT
                     KDIHIIIGSENIYAPIKDISIITATYTMGGKIIGKIGLLGPTRMDYPNLIETVKIYSD
                     NISEILNLLMER"
BASE COUNT          404 a          250 c          167 g          500 t
ORIGIN      
        1 ttcttcctct ttttctcttt cttccaggtc tttttgttca ttatccatgc ccttatctac
       61 atccattatc tcaccccaaa caatatattt actttatctt tccatcagta agtttaagat
      121 ctctgaaata ttatcagaat agattttaac agtctctatt aggtttggat aatccatcct
      181 agtaggacct aataaaccaa ttttacctat aattttccca cccatagtat aggtggcagt
      241 aattatacta atatctttaa taggagcata tatattttca cttcctatta taatatgaat
      301 atccttagtt gtaggacttt gagaaagaat ctctaacatt aacttcttgt cttctacaaa
      361 ggagataaag tctttagctt tttccacatc cttatactct ggaaagttta aaatctttgt
      421 taaaccatct gcatataagt ctacagatac taaatcatca atagaagtat taagtatagg
      481 tataagtttt tccaatatag atttaaactc atacatctcc ttaaatacat tttcatccaa
      541 ctgatttgac aattgatcaa tagtcaaacc gcttaatttg ctatttaaga aattggaaat
      601 aatagttaac tgatctgaag tcactggttt gtctagcttc aatatagagt ttttcacagt
      661 tcctgtttca catactataa ccagtaacac ttctttttca tctaaatcca ccagttggat
      721 ctgtctaatt ttactatttt tcatccttgg agaagccacc actgccgtgt ggctagttaa
      781 ggctgataga atttttgctg aattttgaat tagctcatct atttcccaag attctttcat
      841 tagggcttct ttgattttta atttttttat tatatctgtt gcaattttat ttgacttaag
      901 caattcatcc acataaaatc tataggcctt atcagaaggt accctacctg cagaagaatg
      961 aggtttattt aaatatccta aatcttccaa atctgacatt tcatttctga tggttgcaga
     1021 acttacccct agatcatact ttttagtaat agtcctagag cctataggtt ctgcactact
     1081 tatataacta tttataatag caagcaatac ttttaatttc ctatcatcca gcatgatttt
     1141 cacctcaaat atttgcaacg tggatgttgt tagcactcta ggaccctgag tgctaaatcg
     1201 tacaataata agttatcact tgttttaaaa tttgtcaata ctttctatag aaattggaag
     1261 cttaaggtaa aaaatccatt tccacttgat tggccaagtc taggcctagc ctagttaatc
     1321 a
//
LOCUS       DATGSI010000368         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_403681, whole genome shotgun sequence.
ACCESSION   DATGSI010000368 DATGSI010000000
VERSION     DATGSI010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403681"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="VK031_05710"
     CDS             complement(<1..1665)
                     /locus_tag="VK031_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HLS53446.1"
                     /translation="MKKEEIRITESDSALDVDSLMQEYDLETSRLRKLGGPVAKITSI
                     IAIAMSLFQFYTGGFGTLMSVRQRSLHIIFAFTLGFLLYPATDKSDKEKPSILDYILI
                     LLNIVVFGYLFVFVQEIARKGTNLTAIDMIFGTIAILITLEVTRRVVGPELPIVAIVF
                     LLYARFGPYMPGILAHRGFTFKRIISHMYMALEGILGTPIYVSATFVFMFILFGSFLD
                     KTGVGKFFIDLAFAMTGHTKSGPAMTAVVASGLMGSISGSSVANTVTTGAFTIPLMKQ
                     TGYEPHFAGAVEATASTGGQIMPPVMGAAAFIMADFTGLSYLSIIIAAAIPAILYYFA
                     VGTMVHLEASKLGLKGLPKEQLPKLGETLKKEGYLLIPLISVIVFLVMRFSPNKASFY
                     AIVISVIIALVASAIKGDNSFKLINIVEAFEDGAKGAVGVACACACAGIVVGVVTLTG
                     LGLRIADLIVTLAGGRLLLTLFFTMIASIILGMGLPTTAKYIVLATMAVPAITKLGVN
                     LMAAHLFILYFGVVADITPPVALAAYAGAGIAGANSMQTGFQAFKLA"
     gene            complement(1662..2216)
                     /locus_tag="VK031_05715"
     CDS             complement(1662..2216)
                     /locus_tag="VK031_05715"
                     /inference="COORDINATES: protein motif:HMM:NF020479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HLS53447.1"
                     /translation="MMSKDSNNKYFKGQGIGKLSNTLPYLIILLILIAVIYLLFPVKV
                     LKASNFSNNEYLKAWKIKNGQVFSIEYIHSVEQSPVIENFLVKGRDIILQDTYFHSYG
                     AGLPATTPYKFEITEDGLRVYDINLKIEDLIYRTSGEGREHKLILGNKEYMFLDFSPH
                     RTGVKFDIGYMINLSYFIKEGDLF"
     gene            complement(2197..2253)
                     /locus_tag="VK031_05720"
     CDS             complement(2197..2253)
                     /locus_tag="VK031_05720"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53448.1"
                     /translation="MPIEVHPGAQKYYDEQGL"
BASE COUNT          723 a          471 c          371 g          724 t
ORIGIN      
        1 tgccaattta aatgcctgga atcctgtttg catagagtta gcacctgcaa ttccagcccc
       61 tgcataggct gccaaggcta ctggtggggt aatatctgct acaactccaa aatataggat
      121 aaataaatgg gctgccatta gattaacacc cagtttagta atcgctggta ctgccatggt
      181 tgctagtact atatacttgg ctgtagttgg tagacccata cctagtataa ttgaagctat
      241 cattgtaaag aacagggtta gaagtaatct accacctgct aatgttacta ttaagtctgc
      301 tattctaaga cctaaacctg ttagtgtaac aactccaacc actatacctg cacaagcaca
      361 ggcacaggct actcctactg ctccctttgc cccatcttca aaggcttcta ctatattaat
      421 taacttaaag gaattgtccc ctttaattgc agacgcaacc aaagcaataa ttacacttat
      481 tacaatggca taaaaggatg ccttgttggg actaaatctc ataaccagga aaactataac
      541 tgatattaat ggaataagca aatatccttc cttctttaag gtttctccca gttttggtag
      601 ttgttccttt ggtaagcctt taagtccaag ttttgatgct tctagatgaa ccatggttcc
      661 tactgcaaag tagtataaaa ttgctggtat agccgctgca ataattatac ttaggtaaga
      721 tagccctgta aagtctgcca ttataaaggc tgctgctccc attaccggtg gcattatctg
      781 tcctccagtg gaagctgttg cctctactgc cccagcaaaa tgtggctcat atccagtttg
      841 cttcatcagt ggtatggtaa atgccccagt agttacagta ttagcaacag aacttccaga
      901 aatagatccc attaaacctg atgcaacaac tgctgtcatg gctggcccac tctttgtatg
      961 ccctgtcatg gcaaaggcta aatctataaa gaatttacct accccagttt tatctaaaaa
     1021 tgaaccaaat aagataaaca taaagacaaa ggttgcagat acataaatag gcgtacctaa
     1081 aattccttct agggccatat acatatgaga aataattctc ttaaaggtaa atcctctatg
     1141 tgccaatata cctggcatat aaggaccaaa tctagcatac agtaaaaata ctatggcaac
     1201 tataggaagc tctggtccta caacccttct ggttacctct agggtaatca ggattgctat
     1261 ggttccaaat atcatatcta ttgcggtcag attagttccc tttctagcaa tctcctgtac
     1321 aaacacgaaa agatacccaa acactactat atttaataat ataagtatat agtcaagaat
     1381 tgagggtttc tccttatctg acttgtcagt agcaggatat aataggaaac ctaaagtaaa
     1441 ggcgaatatt atatgtaagg atctctgtct aacactcatt aatgtcccaa atccacctgt
     1501 ataaaactgg aataatgaca ttgcaatagc aattatggaa gtaattttag ctacaggtcc
     1561 tcccagtttt cttagtctgg aggtttccag gtcatattct tgcattaaag aatctacatc
     1621 taacgctgaa tcagattctg taattcggat ctcttccttt ttcaaaacaa atcaccttcc
     1681 ttaataaaat atgacaaatt tatcatatag cctatatcaa attttacccc ggttctatgg
     1741 ggcgaaaaat ctaggaacat atactcctta tttcctaaaa ttaatttatg ttctcttcct
     1801 tccccgctgg ttctataaat taaatcctct atttttaaat taatatcata tacacgtaaa
     1861 ccatcttcag taatttcaaa tttatatgga gtagtggcag gtagtccagc cccataggaa
     1921 tgaaaatatg tatcctgtaa aataatgtct ctgcccttta ctagaaaatt ttctataact
     1981 ggggattgtt ccaccgaatg tatatattct atgctaaata cttgtccatt ctttattttc
     2041 caagccttta aatattcatt attactaaag ttggaggcct ttaagacctt gaccgggaat
     2101 aggagatata ttacagctat taatattaat aaaataatta gataaggtaa ggtattggaa
     2161 agttttccaa taccttgacc tttaaaatac ttattattag agtccttgct catcataata
     2221 tttctgagct ccaggatgta cttctattgg cataccttct agagccgttt ctagttgtaa
     2281 gtctttccc
//
LOCUS       DATGSI010000369         1141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_215440, whole genome shotgun sequence.
ACCESSION   DATGSI010000369 DATGSI010000000
VERSION     DATGSI010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215440"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..>1141
                     /locus_tag="VK031_05725"
     CDS             32..>1141
                     /locus_tag="VK031_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLS53449.1"
                     /translation="MIEKINALIDKNIDIVIAIRRDIHQHPEIAMEEVRTSGIVKAEL
                     EKLGLEVQDKIGNLGVVATLRGKEEGKTLLLRADMDALPIQEQTDLDFKSQVSGKMHA
                     CGHDVHTSILLGVAKVLTELKDEIKGNIKFVFQPAEEDNPTGGARFMIEDGVLENPKV
                     DAAAALHIWGIPLGQVSLKPGTMMAQSDRIFLTVRGKSSHGAEPHNSRDAIVAAGHII
                     TALQTVVSRNVDPMESAVVSLGMISGGSRYNVICDEVKLEGTVRTFNPIISKMMPERI
                     KNIAESVAAAFGCECEVDYVEGYSATVNDRELTESFIDSLQNTLGEENVLIAERPGSG
                     GEDFSEFAMRVPSVYYWLGFASEINEGRTILHNPNL"
BASE COUNT          351 a          195 c          281 g          314 t
ORIGIN      
        1 gagttattcc tataatagga ggttgaaatt catgattgaa aaaattaatg cactaattga
       61 taaaaatata gatatagtta ttgcaattag aagagacatt catcaacacc ctgaaattgc
      121 tatggaagaa gtaagaacct ctggtatagt caaggccgag ctggaaaagc ttggccttga
      181 agttcaggat aagataggaa accttggtgt agtagccacc ctaagaggta aagaggaggg
      241 gaaaactctg ctacttaggg ctgatatgga tgctttacct attcaggagc aaacagactt
      301 ggattttaag tctcaagttt ctggcaagat gcatgcctgc ggtcatgatg ttcacacttc
      361 tattctttta ggagttgcca aggtcttgac tgagcttaag gatgagataa agggcaatat
      421 taaatttgtc ttccagccag cagaagaaga taaccctact ggtggagcca gatttatgat
      481 agaagatggg gtcttagaaa atcctaaggt ggatgcggca gcggccctcc atatctgggg
      541 tataccttta ggacaggttt ccttaaagcc tggtaccatg atggcccagt cagatagaat
      601 cttcctaact gttaggggta agtcttccca tggcgctgaa ccccacaaca gtagggacgc
      661 catagtagct gcaggccata ttattacagc ccttcaaact gttgttagta gaaatgtaga
      721 ccctatggaa agtgctgtag ttagcctagg tatgataagt ggcggtagca ggtacaatgt
      781 tatctgtgat gaagtgaagc tggagggtac tgtaagaacc tttaatccaa tcatttctaa
      841 aatgatgccg gaaaggataa aaaatatagc tgaaagtgtg gcagcagcct ttggctgtga
      901 atgtgaagtt gattatgtgg aaggttattc tgctacagtt aatgacaggg agttgacaga
      961 aagctttata gactccttac aaaacacatt aggtgaggaa aatgtgctta tagctgaaag
     1021 gcctggctct ggtggagaag atttttcaga atttgccatg agggttccct cggtctatta
     1081 ctggctaggt tttgcatccg aaataaatga aggtagaacc atccttcata atcctaactt
     1141 a
//
LOCUS       DATGSI010000370         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_23771, whole genome shotgun sequence.
ACCESSION   DATGSI010000370 DATGSI010000000
VERSION     DATGSI010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23771"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VK031_05730"
     CDS             <1..271
                     /locus_tag="VK031_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015777979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HLS53450.1"
                     /translation="VVRDFVGHGIGQDMHEDPQVLHYGRPGRGLRLTEGMVITIEPMI
                     NVGDYRVSIDDNGWTARTIDGSLSAQYEHTLAITKDGPLILTEQD"
     gene            316..957
                     /locus_tag="VK031_05735"
     CDS             316..957
                     /locus_tag="VK031_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HLS53451.1"
                     /translation="MKKRYTKREEIANGITHGIGIVFGIVALTILLFLAIQNKNIPAI
                     VSFSIYGLCIILMFTSSTLYHSLTKENIKKILRVFDHSSIFLFIAGTYTPVALLTLKG
                     SLRIGILIGIWLIAIAGVIFKIFTFGKFDKYKALSLAIYIGMGWLAIIPIRAIIRATS
                     INFFYWILAGGLLYTLGTLFYSIKRIPYNHAIWHIFVLAASITHFLGIVLYLP"
     gene            1096..1554
                     /gene="tsaE"
                     /locus_tag="VK031_05740"
     CDS             1096..1554
                     /gene="tsaE"
                     /locus_tag="VK031_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HLS53452.1"
                     /translation="MKIKLSNLKETESFGLKLGSLLMPGDILCLNGDLGAGKTTMTQS
                     IGKGLGVDDYITSPTFTLINEYKGRIWVYHFDVYRLENVEDLYDIGFDEYFYGHGVCI
                     IEWAEKIEKLLPEDRIVIDIKKGKETDERILSISGQGSRYKEIIKELDNN"
     gene            1551..>2122
                     /gene="tsaB"
                     /locus_tag="VK031_05745"
     CDS             1551..>2122
                     /gene="tsaB"
                     /locus_tag="VK031_05745"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HLS53453.1"
                     /translation="MKILGLDTSTMISTCAVMEGNEIIGEYTINQNMSHSEKLVPMIK
                     ELLDNLNLRPKDIDLYGVALGPGSFTGLRIGVGTVKTLAHLFDKPIVGISTLEALAYN
                     LPYNKTIVPMIDARRNRVYTGIYTWQEEELKTLKEEEAIEIEKLFEILDDYEDIVISG
                     DGSQVYKDVIRENLGDRIKVATAGANICRAS"
BASE COUNT          747 a          326 c          445 g          604 t
ORIGIN      
        1 agtggtacga gattttgttg gtcatggaat aggtcaagat atgcatgaag accctcaagt
       61 attgcactat ggtaggccag gcagaggctt aaggttaaca gaaggcatgg taataaccat
      121 agagcccatg ataaatgtgg gagattatag ggtttcaata gatgataatg gctggactgc
      181 caggaccata gatggaagcc tatcagctca atatgagcat accctagcca ttaccaagga
      241 tggcccttta atcttaacag aacaggacta gatggatgta ataaaagtaa aaactattaa
      301 ggaaggagga tgcttatgaa aaaaagatac acaaagaggg aagaaatagc caatggcatt
      361 acccatggta taggtattgt gtttggaata gtggctttaa ccattctttt atttttagca
      421 atacaaaaca aaaacatccc agccatagta tcctttagta tttatggcct atgtattatt
      481 ctaatgttta catcctcaac cctttaccat agtttgacta aagaaaatat aaagaaaatc
      541 ctaagagtat ttgatcactc ttccatcttc ctatttatag ctggaaccta cacacctgtg
      601 gccttattga cattaaaagg cagtcttaga ataggaatat taataggaat atggttgatc
      661 gccatagcag gggtgatatt taaaatattc accttcggaa aatttgacaa atacaaggcc
      721 ttatccttgg ctatctatat aggcatgggc tggctggcca taatacctat aagggcaatt
      781 ataagggcca catctattaa ctttttctat tggatattgg caggtggatt attatacact
      841 ttaggaacac ttttttatag tattaaaaga attccttaca atcatgctat ttggcatatc
      901 tttgtattgg ctgccagcat tacccacttt ttaggtatag tcttgtacct accataaact
      961 attaaaaagt atagaaaata taaaggggct gattatcttc agtcctattt ctattttatg
     1021 gacccttgta agctataatt tggtaaacac ttgtggtata attttaagtt agaataaagg
     1081 aaaggttgaa ttaacttgaa gataaaactt tctaatttaa aggaaacaga aagctttggt
     1141 ttaaaactgg gtagcctatt aatgcctgga gatatccttt gtctaaatgg agatctaggg
     1201 gcaggaaaaa ctaccatgac ccaatccata ggcaagggcc taggggtaga tgattatata
     1261 accagcccta cctttaccct aattaatgaa tataagggca ggatatgggt ctatcacttt
     1321 gatgtttata ggctggagaa tgtggaagac ctatatgata ttggatttga tgagtacttt
     1381 tatggccatg gagtatgtat tatagaatgg gctgagaaaa tagaaaaatt actacctgaa
     1441 gataggattg taatagatat taaaaaaggc aaagagacag atgaaagaat actttccatt
     1501 agtggacaag gaagcaggta taaggaaata attaaggagt tggataataa ttgaagattt
     1561 taggactgga tacatccacc atgatttcaa cttgtgcagt catggaggga aatgaaataa
     1621 tcggagaata taccataaat caaaatatga gccattcgga aaaactggtt cccatgataa
     1681 aggagttatt agataactta aatttaaggc caaaagatat agatctatat ggagtagccc
     1741 tagggccagg ctcttttaca ggattgagaa taggtgtagg tactgtaaaa accttggccc
     1801 acctttttga caaacctatt gtaggcatat ctaccctgga ggccctggcc tataatttgc
     1861 cctataacaa aacaatagtc cccatgatag atgccaggcg taacagggtc tatacaggca
     1921 tatatacctg gcaagaagaa gaattaaaga ctttaaaaga agaagaagca attgaaatag
     1981 aaaagttatt tgaaatctta gatgactatg aagacattgt aataagcgga gacggtagcc
     2041 aggtatacaa ggatgtaata agggaaaatt taggggacag gataaaagtt gccacagcag
     2101 gagccaatat atgtagagcc tc
//
LOCUS       DATGSI010000371         4647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_403914, whole genome shotgun sequence.
ACCESSION   DATGSI010000371 DATGSI010000000
VERSION     DATGSI010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403914"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VK031_05750"
     CDS             <1..242
                     /locus_tag="VK031_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HLS53454.1"
                     /translation="GYQCLPSYLEIIKAGSNSKVFLSIREGKFHQVKRMFRSLSMEVL
                     YLKRVAMGNLTLDKGLNPGEYRELKEKELNLLKNI"
     gene            282..842
                     /locus_tag="VK031_05755"
     CDS             282..842
                     /locus_tag="VK031_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6648 family protein"
                     /protein_id="HLS53455.1"
                     /translation="MYSLEVERQRGLFERFFDIRSSLIIQFENGDISKREFLENNYDL
                     VLRTNFKPFLKIDKYEKGMFNYQYYNVLAKYYRMLARDARRSRRDKKYYNYYLNKGNN
                     YYHEKDKAALELLRFLEFKGVEAYFIDVQSKGLRDKLYEIVLLDYKEAIFHSKANWLL
                     EILREEGVFLEEKKRSLIDEYINEKY"
     gene            complement(865..1344)
                     /locus_tag="VK031_05760"
     CDS             complement(865..1344)
                     /locus_tag="VK031_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53456.1"
                     /translation="MKVSEKIKDILYESMDYLIMLGIIVTIALIIGWRLDALFAKESS
                     GPESSQIGVEEGQKDDSYQENGNDPKEDGKIDDEDKEPVQDSNSSDAILTINIPEGSF
                     PSQIASILESQGVIEDSNEFIQKSQEMGLDRKLQSGSFQIEKDASLEELVRIIAKEK"
     gene            1440..2411
                     /locus_tag="VK031_05765"
     CDS             1440..2411
                     /locus_tag="VK031_05765"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLS53457.1"
                     /translation="MKEIIVSQNESGQRIDRFLRKYLDKAPRNFIYRMIRRKNIELNN
                     SRTRPNAIISTGDTIQLYLADETIRKFRSRRDYDTPRVDLDIIYEDNNIILINKKSGT
                     LSHGAGEDKTGNLVDSMIQYLIDKKDFEPDKEKTFTPSICNRLDRNTSGIIIGAKNYE
                     SLRTINQALRHSKIERFYKTIVKGQLKEEIKAKGYLEKNREKNLVKITEDEGVGAKKI
                     LTNIRPLQVSNNYSLLEVELITGRPHQIRSHLAHLGYPIIGDRKYGDKHTNQDLLERF
                     GLKDQWLHGYKIKFDGLERSLDYLNGKIFKAEAHKGYTKIEQKLFSK"
     gene            2423..3334
                     /gene="glsA"
                     /locus_tag="VK031_05770"
     CDS             2423..3334
                     /gene="glsA"
                     /locus_tag="VK031_05770"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HLS53458.1"
                     /translation="MEKRLEKIVRDNYSLTMKGQVASYIPALKRANREDIGLSFMELD
                     GCLYEAGKSRTKFTIQSISKVIALMQALMDNGEAKVFERVGYEGTDEPFNTQYKLDIS
                     PVSKPANPMINSGALVTTGLIKGDGEEKFNKVLELTRKMANNPKIGYNEEVYLSEKST
                     GDKNKSIAYLLKAKGLITGDVDQTLDAYFKQCSIEVDTRDLANIATYLARGCNGLFTD
                     KISKERLTSLILGIMTVAGMYNFSGQYLAKVGIPSKSGVAGGIMAVVPGKIGIGVYGP
                     ALDKYGNSSAGYGIMKDLSKEFNLKLF"
     gene            3448..>4647
                     /locus_tag="VK031_05775"
     CDS             3448..>4647
                     /locus_tag="VK031_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HLS53459.1"
                     /translation="MRKILPWILVIVLIFTGCTGKRNEKTEAESNGDSGEITRQEYTF
                     VYSGEITTLNYLITSSTNEFGVAANLVDSLVDYDKYGVVQPALATDWSVSEDNLVWTF
                     KIREGVKWVTKDGEEYAEVTAEDFVDAMKYILDSENESLTANIAYGVIKNGEKYYNKE
                     IDDFSQVGVKAKDKYTLEYTLEKPVPYFLSMMNYVCFFPVNGKFLAEVGEDFGTDNTK
                     ILYNGAYIMEIFEPQNKRVLVKNEDYWDRDNVHIEVLTAIYNKEADNLAPEMFLRGEI
                     DSASIPSTILNEWMNDQEKAKVIRPNRTSYYSYFYAFNFDPLFPEKYEPENWKKAVNN
                     LSFRKAIFHALDRKAAMLTAEPYDPEKRIHNTITPRNFVNYEGVDYTEMGPLKDIVNR
                     DSFNEELA"
BASE COUNT         1692 a          648 c          955 g         1352 t
ORIGIN      
        1 atggatatca gtgtctacca tcatatttag agattataaa ggcaggatct aattctaagg
       61 tttttttaag tataagagaa ggaaaatttc atcaggtaaa aagaatgttt agaagcctat
      121 ctatggaggt tttatatcta aaaagagtag ccatgggaaa tttaaccctg gataagggtc
      181 taaatccggg agaatacagg gaattgaagg aaaaagaatt aaatctatta aaaaacatat
      241 agtttttatg ctataattaa tattaaatca aattagaatg ggtgtatagt ttggaagttg
      301 agaggcaaag gggcttgttt gaaaggtttt ttgatattag gtcatcttta attattcagt
      361 ttgaaaatgg agacataagt aagagagagt ttctagaaaa caactatgat ctagttctta
      421 ggacaaactt taagcccttt ttaaaaatcg acaagtacga gaagggtatg tttaattacc
      481 agtattacaa tgtgctagct aagtactata gaatgttggc aagggatgct agaagaagta
      541 ggagggacaa aaaatactat aactattact taaataaggg taataattac tatcatgaaa
      601 aggataaggc tgccctagaa ttattaagat ttctcgaatt taaaggggtg gaagcctatt
      661 ttatagatgt tcagtctaaa ggactaaggg ataaacttta tgagatagtc ttattggatt
      721 ataaggaggc catatttcat tctaaggcca actggctttt ggaaatacta agagaagaag
      781 gagtattttt agaagagaag aaaagatcct taattgatga atatattaat gaaaaatatt
      841 aacagactag aaatagtctg ttaattattt ttctttggct ataattctta ccagttcctc
      901 taaactagca tctttttcaa tttggaagct acctgattgt agctttctat caagacccat
      961 ttcttgagat ttctgaataa actcattact atcttctata acaccttgag attctaaaat
     1021 ggaggcaatt tgactgggaa aggaaccttc aggaatgttg atagttaaga ttgcatctga
     1081 agaattagag tcctgaacag gctctttatc ttcatcatct atctttccat cctcttttgg
     1141 atcatttcca ttttcttggt aggaatcatc cttttgacct tcttctacac caatttggct
     1201 actttcagga ccggaagact ccttggcaaa taaggcatcc aggcgccaac ctattataag
     1261 tgctatggta acaataattc ctaacatgat caaatagtcc atagattcat acaaaatatc
     1321 tttaattttt tcggaaactt tcatgttttc acccttattc tatgtataaa ttaagtttgt
     1381 gctgcaatct aatatataat ataattatag cataaagaaa gggaaaaggt gaaactatct
     1441 tgaaggaaat aattgttagc cagaatgaaa gtgggcaaag aatagatagg tttttaagga
     1501 aatacctaga taaggctcct agaaacttca tctataggat gattagaagg aaaaatatag
     1561 agttaaataa ttccaggacc aggcccaatg ctattatatc cacaggggat actatccagt
     1621 tgtatttggc agatgaaacc ataagaaagt ttaggtcaag aagagactat gacacaccta
     1681 gggttgattt agatattata tatgaagaca ataatataat tctaataaac aaaaaatctg
     1741 gtaccctttc ccatggagct ggtgaggata aaactggcaa tctagtagat agtatgatcc
     1801 agtatttaat agataagaaa gactttgagc cagacaagga aaaaacattt actccctcca
     1861 tttgtaacag attagataga aatacatctg gcataataat aggggccaaa aactatgaaa
     1921 gcttaaggac tataaaccag gctctaaggc attctaagat tgaaagattt tataagacca
     1981 ttgttaaagg acagcttaag gaggaaataa aagcaaaagg atatttggaa aagaatagag
     2041 aaaaaaacct agttaaaatc actgaagatg aaggggtagg ggcaaaaaaa atacttacaa
     2101 atattagacc actacaagtt tctaataatt actccctatt ggaagttgaa ttaattactg
     2161 gaaggccaca tcaaataaga agtcatctag cccatttagg atatcctatt ataggtgata
     2221 gaaaatatgg cgataagcat acaaatcaag acctgttgga aaggtttggt ttaaaagacc
     2281 aatggctgca tggatataaa attaaatttg atggcctaga gaggtcatta gattatttaa
     2341 atggaaaaat atttaaggcg gaagcccata aaggctatac taaaattgag caaaaactat
     2401 tttctaaata ggggagataa atatggagaa aaggttagaa aaaatcgtta gagacaatta
     2461 cagtttgact atgaaagggc aggtggccag ctatatacct gccttaaaaa gggctaacag
     2521 ggaagatata ggcctatctt ttatggagct tgatggctgc ttatatgagg caggtaaatc
     2581 tagaactaaa tttactattc aaagcatatc taaggttata gccctaatgc aggcactgat
     2641 ggacaatggg gaggctaagg tgtttgaaag ggtaggctat gaaggcactg atgaaccatt
     2701 taatacccaa tataaactag acatatctcc tgtaagtaag ccagccaatc ctatgattaa
     2761 ctcaggagcc ttggtaacca ctggtctcat taagggagat ggggaagaaa agtttaacaa
     2821 ggtattagaa ttaaccagaa aaatggccaa taatcctaag ataggatata atgaagaagt
     2881 ttatttatcg gaaaaatcaa caggcgataa aaacaaatcc atagcctatc tactaaaggc
     2941 caaaggctta ataacaggtg atgtagacca gaccttagat gcatacttta aacaatgttc
     3001 aattgaggtg gatacaaggg atctggccaa tatagctaca tacttagcca ggggatgtaa
     3061 tggtttgttt actgataaaa tcagtaagga aagattaacc tccctcatat taggaataat
     3121 gactgttgct ggcatgtata atttcagtgg gcaatattta gcaaaggttg gcataccatc
     3181 aaaatcagga gttgccggag ggattatggc tgtagttcca gggaaaatag gcattggggt
     3241 ttatggaccg gccttggaca aatatggcaa ctctagtgca ggttacggaa ttatgaagga
     3301 tttatcaaaa gaatttaatc taaagctctt ttaaaaggcg ctcaggcgtc tttttttatt
     3361 tatcccatta attaattaat aaatattagg ggctattaat atatataaat atatgagtct
     3421 aaataagtat aagttaggag gtagaagatg agaaaaatat taccgtggat tttggtaatt
     3481 gtcctaattt ttacaggatg tacaggcaaa agaaatgaaa aaacagaggc agaatccaat
     3541 ggggactccg gtgaaattac caggcaagaa tacactttcg tctattcagg ggaaattaca
     3601 actttaaatt atctaataac ttccagcacc aatgagtttg gcgtagctgc taacctggta
     3661 gattccctgg tggattatga taaatacggt gtggttcagc cggcattggc cactgactgg
     3721 tcagtgtctg aggataatct agtatggacc tttaagatac gagaaggtgt aaagtgggtt
     3781 accaaggatg gagaggaata tgctgaggtt actgcagaag atttcgttga tgcaatgaaa
     3841 tatatattag acagtgaaaa tgaatcttta actgccaaca ttgcctatgg agtaattaaa
     3901 aatggagaaa agtattacaa taaggagatt gatgattttt cccaagtagg agttaaggca
     3961 aaagataaat ataccttaga atacactttg gaaaaacctg tcccttattt cctatctatg
     4021 atgaattatg tttgtttctt tccagtaaat gggaagtttc tagcagaagt aggggaggat
     4081 tttggaactg ataatactaa aattctgtat aatggggctt atataatgga aatctttgag
     4141 ccacagaata aaagagtatt ggtaaagaat gaagattatt gggatagaga taatgtccat
     4201 attgaagttt taacggccat atataacaag gaggcagata atttagctcc agaaatgttt
     4261 ctaagaggtg agatagattc ggccagtatt ccatctacaa ttttaaatga gtggatgaat
     4321 gatcaggaga aggccaaggt aatcagacct aacaggactt catattatag ctatttctat
     4381 gccttcaatt ttgacccatt atttccagaa aaatatgagc ctgaaaattg gaaaaaagca
     4441 gtaaataatc taagctttag gaaggctatt ttccatgcct tagatagaaa ggctgcaatg
     4501 ttaacagctg agccctatga tccggaaaag aggatacata atactattac acctaggaat
     4561 tttgttaact atgaaggtgt agattacaca gagatggggc ccttaaaaga tatagtaaat
     4621 agggacagtt ttaatgagga attggcc
//
LOCUS       DATGSI010000372         1121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_72491, whole genome shotgun sequence.
ACCESSION   DATGSI010000372 DATGSI010000000
VERSION     DATGSI010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72491"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..>1121
                     /locus_tag="VK031_05780"
     CDS             155..>1121
                     /locus_tag="VK031_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLS53460.1"
                     /translation="MKRENVWSKDFTLITIGTVISAIAWQTINLPLSLTVFDETGSTF
                     LSAILFIASLAPSVVLPILIAPLIDRNPKKIIIVGLDYLTGFMYLLIALVVSITGFNY
                     YLYLFFSLIMGIIGTVYQLAYQAWFPDLIPLGFEQQGFAVSSSIYPSVMVVMSPVAAY
                     LYRTFSLSILFIVVGILTIIAASFELLISNIHNGSQDEEFDFKQYKADLLGGFTYLKK
                     EKGIRNIYGYMSITNGVANGISLMVQAYFQTVSFLSVTMLAFIMSAETAGRIIGGLIQ
                     YKINIPAEKRFGITRFVYIFYESMELILLFIPYPLMIINRFFCGLL"
BASE COUNT          346 a          177 c          211 g          387 t
ORIGIN      
        1 aaaacaagtg gaaggtggcc catcaccagc ttttactatt aggaaggtat cattcaacgg
       61 ccagggataa aagagatatt tatcaggtag tagaggagct tagagaaaaa tattgcaata
      121 aataaataca acaataaaat agtaggtgac aattatgaaa agagaaaatg tatggtctaa
      181 agattttacc ctaattacta taggaactgt tatatctgcc atagcctggc agaccataaa
      241 tttaccctta agcctgacag tatttgatga aacaggatct acttttttgt cagcaatttt
      301 atttatagcc agtttggcac ctagtgtggt cctacctata ttaatagctc cccttataga
      361 taggaatcct aagaagataa taatagtagg cctagattat ttaactggat ttatgtatct
      421 acttatagct ctggttgtaa gtattactgg ctttaactat tatttatacc tgtttttcag
      481 cctaattatg ggtataatag gtacagttta ccaattggcc tatcaggcct ggtttccaga
      541 cctgataccc ttaggttttg aacaacaggg ttttgctgtt tcatcatcta tatacccttc
      601 tgttatggta gttatgtcgc ctgtagcagc ctatctttat aggacatttt ctttaagtat
      661 cctatttata gtggtaggaa ttcttaccat aattgctgcc agtttcgaac ttctaatctc
      721 caatatacat aatggaagcc aggatgaaga gtttgatttt aagcaatata aagctgacct
      781 tttaggcggc tttacctatc ttaaaaaaga aaaggggatt agaaatatat atggatatat
      841 gagtattacc aatggagtag ccaatggcat ttccctgatg gttcaggcct actttcaaac
      901 tgtaagcttt ctttcagtta ccatgttggc ctttataatg agtgcagaaa ccgcaggtag
      961 gattataggt ggtctgatcc aatataaaat aaatattcct gctgagaaaa ggtttgggat
     1021 tactaggttt gtttatattt tttatgaaag tatggaattg attcttttat ttattcctta
     1081 tcctttgatg ataattaata gattcttctg tggcctattg g
//
LOCUS       DATGSI010000373         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_687262, whole genome shotgun sequence.
ACCESSION   DATGSI010000373 DATGSI010000000
VERSION     DATGSI010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687262"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /gene="lpdA"
                     /locus_tag="VK031_05785"
     CDS             <1..1284
                     /gene="lpdA"
                     /locus_tag="VK031_05785"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HLS53461.1"
                     /translation="GCIPTKTYFKNAQIMNSLKRSKEYGIEVDGYRLDGKALLARKEK
                     IVDTLRDGVERLISSYKNIEYLSGKGKLKAKDTVSVELLNGDRQEIKVDNIIIATGSR
                     PTMADIKGIDLLGVIDSDELLELDTIPESLIVVGGGVIGLEFASIYQELGSQVTLLAS
                     RILKDIDRELTRRLPVYFKKQGMKVHTQIRAKEIRKEGDQLRVIAKHKSRDRSVEAVG
                     DYVLIASGRGPVTEGLNLDQVGVDYDTGGIKVDQNFETTVEGIYAIGDVNDVGIQLAH
                     VASNQGIYVVEKLMDQDPDINLDVYPNCVFTIPEVAYVGLMEDELREKGLDFKTSKFL
                     FAANGKALSLGESDGFVKVFSSKSDNKILGVTIVGPNANDLIHEGALAIANQLDVDSI
                     NRTIHAHPTLSEAFMEAVHGLDNRAIHMAPPRKRR"
     gene            1344..1964
                     /locus_tag="VK031_05790"
     CDS             1344..1964
                     /locus_tag="VK031_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLS53462.1"
                     /translation="MKTKKDAVLILILLIVFLSLGIMVKGSKEGILFDVTILDFLHKD
                     TNPTILSLMKFISFIGSEKFLFPAVGLVILYTLIEKRYYVSKILLINTLGSFILNHLL
                     KQLFQRTRPLAYILVKQGGLSYPSGHSMVTMSMYLSIAYLLTRNLEERGKKTLVYTIA
                     LLYILSMGLSRMYLGVHWPTDIIGGYIAGYLLHYFSVRLASRRNYI"
     gene            complement(2050..2937)
                     /locus_tag="VK031_05795"
     CDS             complement(2050..2937)
                     /locus_tag="VK031_05795"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLS53463.1"
                     /translation="MLDFRLNSFLTLCQVLNYTKTAEILHITQPAVSQHIRYLEEYYG
                     VKLFLYKGKNLRLTDAGKKLYSFATAMKTSSDKLKTTLKLAEEESSPLKFGTTLTIGE
                     YTMTYVLRKLLSDFPQLSISMEVGNTELLLHKLKEGEIDFALLEGHFDKSQYQAILLS
                     LENFIGICSNNHPFNNRRVNFDEILNERLILREEGSGTRNIFEQILYEHNYTMEAFKQ
                     VVEIGNMETIKSLVKENLGISFMYEAAVKEELNEGSLVRIDLEDFQIKREFNFVFLKD
                     SLHAQEYLKWFKYFDSKLK"
     gene            3044..>3355
                     /locus_tag="VK031_05800"
     CDS             3044..>3355
                     /locus_tag="VK031_05800"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HLS53464.1"
                     /translation="MNFVKDYIPGILLTIGLALIGKYISGFIPMGLISPGVFSLLLGM
                     CMNPFFMQYDFFEKGTGFVSKRILRLAIILMGATLSFGQVFQVGKYSLFVMVFTLFTA
                     FF"
BASE COUNT         1127 a          531 c          661 g         1036 t
ORIGIN      
        1 ggttgtatcc ctacaaagac ctatttcaag aatgctcaaa taatgaattc tctaaaaaga
       61 tcaaaggaat acggtataga agtagatggc tacaggctag atggaaaagc cctattggca
      121 agaaaagaaa agatagtaga tactttaaga gatggggtgg aaaggcttat atcctcctat
      181 aaaaatatag aatacctaag tggtaagggt aagcttaagg ccaaggatac tgtttccgtt
      241 gaactgctaa atggagatag gcaggaaata aaggtggata atataattat agccacaggg
      301 tctaggccaa ccatggcaga tattaaggga atagatcttc taggagttat agactctgat
      361 gaactgctgg agcttgatac cattccagaa agtctaattg tagtaggcgg aggagtcata
      421 ggattagagt ttgccagcat ctatcaagaa ctaggttctc aggttaccct gctggcatca
      481 aggatactga aggatataga tagggaactt accaggagat tgcctgtcta ttttaagaaa
      541 caaggaatga aagtacacac ccagattagg gctaaggaaa taagaaaaga aggggatcaa
      601 ttaagggtaa ttgccaaaca taaaagtaga gataggtcag ttgaagcagt aggagattat
      661 gtattaatag cttctggtag agggccagta actgaggggc ttaacctgga ccaggttgga
      721 gtagattacg ataccggtgg tattaaggtt gatcaaaact ttgaaacaac agtagaagga
      781 atatatgcca ttggagatgt gaatgatgtg ggtatacagt tggcccatgt tgcatccaac
      841 cagggtatat atgtggttga gaaacttatg gatcaggatc cggatattaa tttagatgtt
      901 tatccaaatt gtgtatttac tataccagag gtggcctatg taggattaat ggaagatgaa
      961 ctaagggaga agggcctaga tttcaaaaca agtaaattct tatttgcagc caatggtaag
     1021 gccctgtcct taggtgaaag tgatggattt gttaaggtat tttcatctaa atcagataat
     1081 aaaatactgg gggtaaccat agtaggtcct aatgccaacg atttaatcca tgaaggggcc
     1141 ctggccatag ctaatcaatt ggatgtggac agcataaaca ggacaataca tgcccatcct
     1201 actttaagtg aagcctttat ggaggcggtc catggcctag ataatagggc tatccatatg
     1261 gcaccgccaa gaaaaagaag atagtaatta gagacaaggg ccttccttgt ctctaaatta
     1321 aattgctaaa gaaggagaag gccatgaaaa ctaaaaaaga tgcagtattg attcttatat
     1381 tattaatagt atttttatcc ctgggaataa tggtaaaggg ttccaaggag ggtatactat
     1441 ttgatgttac tattttagat tttttacata aagataccaa tcctacaatt ctttctttga
     1501 tgaaatttat atcctttata ggatcagaaa agttcctatt tccagcagta gggctggtaa
     1561 tcctatatac actaatagaa aaaagatatt atgtctctaa gattttatta ataaatactt
     1621 taggtagctt tatattaaac cacctattaa aacaactttt tcaaaggacc aggcccttgg
     1681 cctatattct agtaaagcaa ggtggccttt cctacccaag tggccattct atggtgacca
     1741 tgagtatgta tctaagtatt gcctacctat taacaagaaa tctagaagaa aggggaaaga
     1801 agacattagt ttacacaata gccctactat atatcctaag catgggactt agcaggatgt
     1861 atttgggagt ccattggcct acagatataa taggaggcta tatagcaggg tacctattac
     1921 actatttcag tgtcagattg gcctcaagaa ggaattatat ataaaattaa catttaaata
     1981 taaaaacagg atgaaagcaa accttttatc ctgtttatta tatttatgca agatataaat
     2041 ccaataggct tattttaatt tagaatcaaa atacttaaac catttaagat attcctgggc
     2101 gtggagacta tcctttaaga atacaaaatt aaactctctt ttaatctgaa agtcttctaa
     2161 atcaattcta actagagaac cttcatttaa ctcttcttta actgccgcct catacataaa
     2221 actaatacct aggttttcct ttaccagact ctttatagtc tccatatttc ctatttcaac
     2281 cacctgctta aaggcttcca tagtataatt gtgctcataa agtatctgtt caaagatatt
     2341 cctagttcct gaaccttctt ccctaagtat taacctttca tttagaattt catcaaaatt
     2401 aacccttcta ttattaaagg ggtggttatt ggaacatata ccaataaaat tttccaagga
     2461 taataatatg gcctggtact ggcttttatc aaaatgtcct tccaataagg caaaatcaat
     2521 ttctccttct ttaagcttat gaagaaggag ttctgtgttt ccaacttcca tacttatact
     2581 taattgggga aagtcagaaa gcaattttct caacacataa gtcatagtat attcacctat
     2641 ggttaatgtg gtaccaaatt ttaaagggct agattcttcc tcagccagct taagagtggt
     2701 ctttaacttg tcagagctgg ttttcatagc tgtggcaaag ctataaagtt ttttgcctgc
     2761 atcagtcagg cgcaagtttt tacccttgta aagaaaaagt tttaccccat aatattcttc
     2821 caaatacctt atatgttgtg atactgcagg ttgggtaata tggagaattt ctgccgtttt
     2881 ggtatagttt agcacctggc atagggttaa gaagctattg agtctaaaat caagcataag
     2941 atcacctata agataaaatt attaatatat aaaaatacat aattagattt tataccacaa
     3001 ttagaatata atcaataaca gagcttaaaa aggagtgacg gaagtgaatt ttgttaagga
     3061 ttatatacca gggattttat taacaattgg actagcctta attggaaagt atataagtgg
     3121 atttatacct atggggctaa taagtccagg agtattctcc ctgttgctag gtatgtgtat
     3181 gaatcccttt tttatgcagt atgatttctt tgaaaagggg acaggatttg tatctaaaag
     3241 aattttaagg ttggccatca tacttatggg tgcaacctta agttttggcc aagtatttca
     3301 ggtggggaaa tattccttat ttgtaatggt atttacccta tttactgctt ttttt
//
LOCUS       DATGSI010000374         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_546520, whole genome shotgun sequence.
ACCESSION   DATGSI010000374 DATGSI010000000
VERSION     DATGSI010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546520"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /gene="gatB"
                     /locus_tag="VK031_05805"
     CDS             complement(<1..1192)
                     /gene="gatB"
                     /locus_tag="VK031_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HLS53465.1"
                     /translation="MTYKTVIGLEIHVELMTKTKIFCSCTNEFGGEANTHCCPVCLGM
                     PGALPRLNEQVLEYGMKAGIAFNSNISRRSKMDRKHYFYSDLVKGYQTSQEEIPLCQG
                     GYLEIETEKGPKKIRLNRIHIEEDTGKSIHTETEDTLLDYNRAGVPLIEIVTKPDINS
                     AEEARLFLEKLRSTLKYIEVSDCKMEEGSLRCDINVNVIDEINGRKSNISEIKNLNSF
                     RAAVRAIEYEEKRHIDLLKAGKNTEAETRRWDEVEGKTVIMREKGEGVDYRFLPDGDI
                     LPIEISDKWIEKIKNSLPELPHAKKKRFMKDYGLTEYDAGVLTQSRDIASFYEETVNH
                     INEPDLVSNWVMGDVLRRLNEEDLEIDSLKFDAKALADLIRLVKDGKINNNTGKKVLR
                     TMFEK"
     gene            complement(1193..>1599)
                     /locus_tag="VK031_05810"
     CDS             complement(1193..>1599)
                     /locus_tag="VK031_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HLS53466.1"
                     /translation="DLDQLFVRSRSEAFGKEVKKRILLGTYLLSEEAREKYYKKAQKI
                     RTLLINDFNQVFSQYDLILTPTSPRLARPVEGSKDTYKNYIEAVNLAGLCAISVPTSN
                     DGIEPVGLQIIGDRFNEEKIIKAALAYEGMVE"
BASE COUNT          419 a          356 c          215 g          609 t
ORIGIN      
        1 ccttttcaaa catggttctt aatactttct ttccagtatt gttatttatt ttaccatcct
       61 taaccagcct aattaaatct gccaaggcct ttgcatcaaa tttaagtgaa tctatttcta
      121 aatcttcttc gttaagtctg cgtaaaacat ctcccattac ccaattacta actaggtccg
      181 gttcatttat atggttaact gtctcctcat aaaaggaggc aatatcccta gactgggtca
      241 gtacacctgc atcgtattca gttaaaccat agtctttcat aaatcttttc tttttagcat
      301 ggggcaattc aggtaaagag tttttaattt tttctatcca tttatctgat atttctattg
      361 gcaggatatc tccatcaggt aaaaatctat aatctacacc ttcacctttt tccctcatga
      421 ttacagtttt tccttctact tcatcccatc ttctggtttc tgcttctgta ttttttcccg
      481 cctttaatag gtctatatgt cttttttcct catattctat agcccttact gctgccctaa
      541 aagagtttaa gtttttaatc tctgaaatat tgcttttcct accatttatt tcatctatca
      601 catttacatt tatatcacat cttagggagc cttcctccat tttacaatct gatacttcta
      661 tatactttaa ggtagaccta agcttttcta aaaataacct ggcctcctct gctgaattta
      721 tatcaggctt tgttactatt tcaattaaag gtaccccagc cctattataa tctaataagg
      781 tatcctctgt ctctgtatga atagatttgc cagtatcttc ttctatatga atcctattta
      841 ggcggatctt tttaggtccc ttttctgttt caatttccaa atatcctcct tgacacaaag
      901 gaatttcctc ctgagatgtt tggtatcctt tgaccaggtc agaataaaaa taatgtttcc
      961 tatccatttt agatcttctg gatatatttg aattaaaggc tatacctgct ttcattccat
     1021 actctaaaac ctgctcatta agtctaggta aggcccctgg cattcctaaa caaacggggc
     1081 aacaatgggt gttggcctct ccgccaaatt cattggtaca actacaaaat atttttgtct
     1141 tagtcattaa ctccacatgg atttcaagac cgataacagt tttataagtc acttattcca
     1201 ccatcccttc ataggccaag gctgctttaa ttatcttttc ctcattaaac ctgtctccta
     1261 ttatctgtaa acctacaggt tctatcccat cattactggt aggtacactg attgcacata
     1321 agccggccaa attaactgct tctatataat ttttgtaagt atccttactt ccctctactg
     1381 gcctggccag tcttgggctg gttggtgtca ggataaggtc atattgggaa aagacctgat
     1441 tgaagtcatt tattaacaag gttcgaattt tttgtgcttt tttgtaatat ttttccctag
     1501 cctcttcact caatagatag gtgcctagaa gaattctttt ctttacttcc ttgccaaagg
     1561 cttcacttct acttcttaca aataattgat ctaaatcat
//
LOCUS       DATGSI010000375         1356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_72536, whole genome shotgun sequence.
ACCESSION   DATGSI010000375 DATGSI010000000
VERSION     DATGSI010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72536"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VK031_05815"
     CDS             complement(<1..228)
                     /locus_tag="VK031_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HLS53467.1"
                     /translation="MGYLNNVVGYRDDILLNRSIIKKDYFALIEPDGLVKNNIPGFEN
                     CAVTILASPKLGASFVDYLVTLEEDGRNKEGF"
     gene            complement(248..>1356)
                     /gene="allC"
                     /locus_tag="VK031_05820"
     CDS             complement(248..>1356)
                     /gene="allC"
                     /locus_tag="VK031_05820"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR03176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HLS53468.1"
                     /translation="ALKDRLESMGMETKFDSIGNLFGRVKGSEHPDKTIATGSHIDTV
                     INGGQLDGQLGIIGGMLAIKNLVKKYGEPKKNLEIISMAEEEGSRFPYVFWGSKNIFG
                     LANKEDVIEIKDNEGNYFVDEMRKCGFDFKQDDSPREDIEAFIELHIEQGNFLEMENK
                     SVGIITSIAGQVRYEVVLKGQSNHAGTTLMKYRKDTIEAYSKIVSNSLISAKEEGDPL
                     VLTFGHITVKPNTVNVVPGETLFTIDCRHTNSLELSKFTGELEERMKDIAYELGIKIE
                     INKWMDEAPVPMDKEVISTIEKACKRLNINYKLMHSGAGHDSQIFAPRVPTGMIFVPS
                     IGGISHNPAEDTKIEDLAQGILALEASLYELAYK"
BASE COUNT          371 a          302 c          173 g          510 t
ORIGIN      
        1 aaatccttcc ttgtttctac cgtcttcttc cagagttact aagtaatcaa caaaggaagc
       61 accaagttta ggtgacgcta atattgtcac tgcgcaattt tcaaatcctg gtatattatt
      121 tttaactaat ccatctggtt caattaatgc aaaataatct ttcttaataa ttgatctgtt
      181 taataaaata tcatccctgt aaccaacaac attatttaaa taacccattc aacaaactcc
      241 ccctttttta tttgtatgct aattcatata ggctggcctc caatgcaagt atcccctggg
      301 ccaaatcctc tatttttgta tcttcagccg gattatggct tatgcctcct atgctaggaa
      361 caaatatcat cccagttgga actctagggg caaatatttg tgaatcatga cctgcaccac
      421 tgtgcataag tttgtagttt atatttaatc ttttacatgc tttctctatg gtggaaataa
      481 cttctttgtc catgggaact ggtgcttcgt ccatccattt atttatttca atcttaattc
      541 ctaattcata tgctatatct ttcattcttt cctctaattc accggtgaac ttagaaagtt
      601 ccagactgtt agtatgtcta cagtctatag tgaaaagggt ttcacctggt acaacattta
      661 ctgtatttgg ttttaccgtt atatgcccaa aagtgagaac caagggatct ccttcttctt
      721 ttgcacttat tagggaatta cttacgatct ttgaataggc ttctattgta tcttttctgt
      781 acttcataag ggttgtcccc gcatggttcg actgtccttt taagactact tcataccgaa
      841 cctgtcctgc aatacttgtt ataattccta cagatttatt ttccatttct aaaaaatttc
      901 cttgttctat atgaagttca ataaaagctt ctatatcttc tctaggcgaa tcatcttgct
      961 taaaatcaaa tccacatttt ctcatttcat caacaaagta gttcccctca ttgtctttga
     1021 tctctattac atcctcttta tttgctaatc caaatatatt cttacttccc caaaacacgt
     1081 atggaaatct acttccctct tcttcagcca tagatataat ctctaaattc ttttttggtt
     1141 ctccatactt cttgaccaaa tttttaatag ccaacatgcc tcctatgatc cctaattgtc
     1201 cgtctaattg tccgccattg ataactgtat ctatatgtga accagttgct atggttttat
     1261 ctggatgctc agatcctttt actcttccaa ataagttacc aatactatca aatttagtct
     1321 ccatgcccat agattccaat ctatctttca aagcat
//
LOCUS       DATGSI010000376         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_641338, whole genome shotgun sequence.
ACCESSION   DATGSI010000376 DATGSI010000000
VERSION     DATGSI010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641338"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..734
                     /locus_tag="VK031_05825"
     CDS             177..734
                     /locus_tag="VK031_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLS53469.1"
                     /translation="MKNLFKELDMYFIEIINKKMKNKFLDKFMYRITDLGGAIFTTVF
                     STILLIFGDNRMKRIGLEALMVLGLSQLIVQIFKIGLGRERPYNTLLDLNTFDIYLKD
                     YSFPSGHTTASFSIATTLALNISGIWPVVFFLALIIGISRIYLAVHYPTDVLAGIILG
                     ISSSLAIHFHLYHYVEAIGRIIGIF"
     gene            862..2964
                     /locus_tag="VK031_05830"
     CDS             862..2964
                     /locus_tag="VK031_05830"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HLS53470.1"
                     /translation="MNIVELELKGLNCASCADKIERKTGELEEVNSANLDFVTKKLKI
                     EVKNNKDLDQVIKDTKAIVKRLEPDVEVVEYRDNHGHAHHHGDVHKSDLIKIIIGGLL
                     FAIPFILKLEGLAKFSVYLLAYLIVGYEIIIRAGKNIAAGMPFDENFLMTIATIGAFI
                     IGEYPEGVAVMLFYQVGEFFQGLAVNNSRRSIKSLMDIRPDYANLVQGEETVTVDPAD
                     VHIGDYIMVRPGERVPLDGVVVEGKSQVDTSNITGESVPRTVEAGEEIFSGVVNNTGL
                     LKIQVSKEFGESTVSKILDLVENASSKKAPMENFITKFARYYTPAVVFIALGIGLLPP
                     LFFQGDLRAWAYKAFIFLVMSCPCALVISIPLGFFGGIGAASKNGILVKGGNYLEALD
                     DVETLVFDKTGTITKGTFNVTEIYSYEDYSKEEILELAAYGESYSNHPIALSIVEAYG
                     KEIDKEYIRNYQEISGKGIQVSIDKADILLGNRRLLDDNNIAVNEIDSIGTVVYVAKD
                     GSHIGTIIVSDEIKEGMTDTIKALKKAGIRKTVMLSGDNEATTVKVGELAGIDVAYGN
                     LLPQDKVSQLEKILQEKEEKDRVAFVGDGVNDAPVLARADIGIAMGALGSDAAIEASD
                     IVIMTDEISKILTGKKIARKTRRIVTQNIVIAIGVKLVVLGLGAAGMATMWQAVFADV
                     GVSLIAILNSIRTLRVED"
BASE COUNT         1061 a          448 c          640 g          951 t
ORIGIN      
        1 tatgtatgtc ctattgcatc caactgctcc tgacttaaat tcaaaatatc acccacttta
       61 tttgtcaaca taaataagac ttttaattct taaaatttac ccttccttat atgttttctt
      121 tattactgcc aggatatatt atataataac atataaaata ttgtaggtga aattagatga
      181 agaacttatt taaagaacta gacatgtact tcatagaaat aatcaataaa aaaatgaaaa
      241 ataagtttct agataaattt atgtatagaa ttactgactt aggtggtgcc atatttacca
      301 ctgtattttc aacaatctta ttaatctttg gcgataatag aatgaagaga ataggcttgg
      361 aggccttgat ggtattaggc ttaagtcaat taattgttca aatctttaaa attggtttag
      421 gtagagaaag accttataat actttgttag atctaaacac ctttgatatt tatctaaagg
      481 attactcctt cccttcaggc catacaacgg ccagcttttc catagccacc accctggccc
      541 taaatatatc cggcatctgg cctgtggtat tttttttggc cttgattata ggaatttcta
      601 gaatttatct ggcagttcat tacccaacag atgtattagc aggtattata ttaggtatat
      661 ccagtagttt agcaatccat tttcatttat atcattatgt ggaagcaata ggaagaatta
      721 taggcatctt ttgaaaagta ccctaattat ttttatataa aaaatatacg taccatattg
      781 acaaccttta aaatctagaa tataattata ttaacatatg aacaattatt catatgagtt
      841 attattaaag gggacattaa aatgaatata gtagaattag aattgaaagg cttaaactgt
      901 gcgtcctgtg cagacaaaat cgaaagaaag actggtgaat tagaggaagt taattctgcc
      961 aatctagatt tcgtaactaa aaaattaaaa attgaggtta aaaacaataa ggatctagac
     1021 caggtaataa aggatacaaa ggccattgtt aaaaggctgg agccagatgt ggaagtcgtt
     1081 gagtataggg ataatcatgg gcatgctcac caccatggag acgttcataa aagtgattta
     1141 attaaaatta taatcggtgg cctattattt gccataccct ttattctaaa attagaagga
     1201 ctggctaagt tttctgtata tttactggcc tatttaattg taggatatga aataataata
     1261 agggctggta aaaatatagc agccggtatg ccttttgatg aaaacttcct gatgaccata
     1321 gcaactatag gggcctttat tataggcgaa tatcctgaag gcgttgctgt aatgcttttc
     1381 taccaagtgg gagagttttt ccaaggcttg gctgtaaaca attctagaag atctattaaa
     1441 tctttaatgg atattaggcc tgattatgcc aatcttgttc aaggtgagga aacagttaca
     1501 gtagacccag ctgatgtgca tataggagat tatattatgg taaggcctgg tgagagggta
     1561 ccactagatg gtgtagttgt agaaggaaaa tctcaggtag acacatcaaa tataacagga
     1621 gagtcggttc ctagaactgt agaggcagga gaagagatct tctcaggagt tgtaaacaat
     1681 actggacttt taaagataca agtaagcaag gagtttggag aatcaacagt atcaaaaatc
     1741 ctggatttag ttgaaaatgc ttcaagcaaa aaggcaccta tggaaaactt tattactaag
     1801 tttgccagat actatacacc ggcagtagtt tttattgcct taggtatagg tctattaccc
     1861 cccttatttt tccagggaga tttaagggct tgggcctata aggcatttat atttttagta
     1921 atgtcctgtc cttgtgccct tgttatttct ataccactag ggttctttgg tggaattggg
     1981 gcagcttcca aaaatggtat cctggtaaag ggtggtaatt atctagaagc cttagatgat
     2041 gtggaaacct tagtttttga taaaacaggt actattacca aaggtacttt taatgtgaca
     2101 gaaatatata gttatgaaga ctattcaaag gaagaaatat tagaattagc agcctacgga
     2161 gaaagttatt ctaaccaccc aatagcccta tctatagtgg aagcttacgg aaaggaaatt
     2221 gataaggaat atattagaaa ttatcaagaa atttctggta agggaatcca ggtaagtata
     2281 gataaggcag atattctgct tggtaataga agattattgg atgataacaa tatagctgta
     2341 aatgaaatag attctatagg tactgtagtt tatgtggcca aggatggtag tcatataggt
     2401 actattattg tatctgatga aattaaagaa ggcatgacag atactattaa ggccttaaag
     2461 aaggctggaa ttagaaagac cgtcatgtta tcaggagaca atgaagctac tactgttaag
     2521 gtaggagaac tggcaggaat agatgtggcc tatggaaatc ttctacccca ggataaggta
     2581 agccagctgg agaaaatcct tcaagaaaaa gaagagaagg atagggttgc ctttgtagga
     2641 gatggggtaa atgatgctcc tgtactggcc agggcagaca ttggtatagc catgggggcc
     2701 ttaggttcag atgcagccat tgaagcttca gatatagtaa taatgacaga tgaaatttct
     2761 aagatcctaa ctggtaaaaa gattgctaga aaaactagaa ggattgtaac ccaaaatata
     2821 gtaatagcca taggggtaaa actagtggta ctgggcctag gggcagcagg aatggccacc
     2881 atgtggcagg ctgtatttgc cgatgtaggc gtatccttaa tagccatttt aaattcaatc
     2941 agaacattaa gagtagagga ttaacctcta cttttcttct tttttgtgat acactataat
     3001 ataaggaaac atggggtgta taagttggta gataataggg atgaaaagag aaggcggaat
     3061 tatcttatcc tagctgaaaa cttttatttg gatgaagata
//
LOCUS       DATGSI010000377         5758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_641351, whole genome shotgun sequence.
ACCESSION   DATGSI010000377 DATGSI010000000
VERSION     DATGSI010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641351"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="VK031_05835"
     CDS             <1..1508
                     /locus_tag="VK031_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HLS53471.1"
                     /translation="IGDYVPRLMLLIEKYVRDLNKYDGAAKSEVYDILIQIEDVDMEV
                     SREEYFDIILSYIRRKRILRGQAQPGYAFATNFQTAAYTGRKHLYLIGLDSDSLSNKT
                     MESPILLDLMRNSISKHLPFANEAYRYKKYKIRELLTAGFESINIGYSNFDTVEVRAK
                     SPSQIYTELREDYETKKEKEVEKIWSIYGRDLASSGTALETLAECSRKAFFRYRLKLY
                     PLDDVEVKVDRWLDPLQRGTLIHRILNAYFDLDTDGENRQALVDIVEKECVMMQEDEP
                     YILYEVYLREKEEIYNICKNIIDLTEADTEWEILINELAFGTKDRKFNKIFGVLPKIK
                     VDIADTSIQVAGSIDRIDVHKENPNKLRIVDYKTGNRSYFERKLRENIGSRGKPVYDY
                     SETEKFQHYIYMKALEEILNTRRDLFPNPEIQSFKYVFADNRSSSVIKIDFNQDFMED
                     IEERIKYLINMDILLADKDVAYDWERYGKCNYCDYKLICMADKTLQIKEVE"
     gene            1509..5015
                     /locus_tag="VK031_05840"
     CDS             1509..5015
                     /locus_tag="VK031_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HLS53472.1"
                     /translation="MEKQDLIQREMIKREEIMNSFNTNYLLEAGAGAGKTTIIVERII
                     NQLIHTDTEPANIVAITFTKAAATELAERIQLKALDYLQDVEDHRIRRKLESIDEIFT
                     GTIHSFCDLMLREMPFEANITPGYEIVEEEADFHNKVWYDFIRNKEGDYKDKIDLLEK
                     FSIDYRELRDGALVAMSNPDIDFVGYEDFNLTYAELEEEFYKIKKEYHKLNPSYIKRS
                     PNLSKLLRAILEEDRSLDYFIEDIFKECPKYELDENTIYERIIYKKNLDNPNRQEYMD
                     FISRVFHLYSTLNKLGYNTCTDFINMAVDYKEKNYRGKLTFNELLYKASQLIQNSSTA
                     RQHFKEKYKYFYIDEFQDTDPMQAELIFYLTDQENEGVRKSWVDCEPRAGSLFVVGDP
                     KQSIYRFRRADITIYNQVKEILAQHGEVVYLDINFRSSDNICNWVQSTFKKEEGGFAF
                     ASKATDTQAGFEKILSLWDDEIIDKGQTYGRKRTNGIYYYDNTRIMDEDVQIDEAEYV
                     ANLVENIMESYYLVEKVRKSTIGIEENEFYNQVRLVEPKDFMILTKTNKETGVYLRAL
                     KDKGIPALLAGEKTLGDTREVLNLFILLDALLDYRDSIKVVAALRNCFYLDLATIDLF
                     MEDIKDRSNILYTDIKIKHSKVEKAILYMREIASPMRRLSPITFIEKLIKNRFGIFNT
                     DREYSTLEERDAESALAQTVEILKAAGCDSIYQVRDELERLVKEQVNYELPISTEYAN
                     NAIRVMNIHKAKGLEANIVILVGRDKKDNLGRDSHYVEKDMYGKILGYLVYKRDYKLL
                     GPKEKENKERENLFKRAENERLLYVAATRAKTSLILAKSSDEEKYLYPLSQRVEEELV
                     PTIKKVENQDIGLGKGKREELEETKELRDKKIILEPSFISLSPSDFVKGKLEEGNKYR
                     VGLSPVFTSFKKENLKDRALGPRGSVFGTIIHRALEVLIKGSRSLGKVDKEIIEYATN
                     ISINESINKLEINKTNLNLLCADSKKVEEMLNTKDKFGSRPVREKIKKEIRPYLETIL
                     INFANNKEIKKLFKEARQIFTELPFSINVKDNVLTKLADFLNREEMQSFMAKDKLVTG
                     IIDLVIQSEDNSWTIIDYKSDIITNENHHARLVKRYEGQLRAYKIFLEEILKEEKVQV
                     DRLILYSTFKDQLIYLEEPALV"
     gene            5245..>5758
                     /locus_tag="VK031_05845"
     CDS             5245..>5758
                     /locus_tag="VK031_05845"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HLS53473.1"
                     /translation="MLVTGKELLLDARENKYAIGAFNVNNMEIVQAIIEAAEELNSPV
                     ILQASQGGLGYAGVEYIAALGKLAGNNAKVPVVLHLDHGTDFDQVMQCIRHGFTSVMI
                     DGSRFPLEENIAFTKKVVEIAHSVGVSVEAELGKIGGTEDHITVDEKDATLTDPEEAK
                     RFVEETNVDYL"
BASE COUNT         2159 a          750 c         1270 g         1579 t
ORIGIN      
        1 aaattggtga ctatgtgcct aggttaatgc tacttataga aaaatatgtt agagatttaa
       61 ataaatatga tggagctgct aaaagtgagg tatatgatat tctcattcag atagaagatg
      121 tagatatgga agtaagtagg gaggaatatt ttgatataat cctatcctat ataaggagaa
      181 aaaggatcct aaggggacag gcccagccag gctatgcctt tgccactaac ttccagacag
      241 cagcctatac aggtagaaaa catctatacc taataggtct ggatagtgat tccttgtcta
      301 acaaaacaat ggaatctccc attttattgg atctaatgag aaacagtatt tcaaaacatc
      361 tgccctttgc caatgaagcc tatagatata aaaagtataa gattagagag ctattaacag
      421 caggctttga aagtattaat ataggatatt ccaactttga tacagtggaa gtaagggcca
      481 aatcaccttc tcaaatctat actgagttaa gggaggatta tgagactaaa aaagaaaaag
      541 aagtagaaaa gatatggagc atttacgggc gagatctggc aagctcaggt acagccttag
      601 aaaccttagc tgaatgtagt aggaaggcct tttttagata tagattgaag ctataccccc
      661 tggatgatgt ggaggttaaa gttgataggt ggctggatcc cctgcaaaga gggaccctta
      721 ttcataggat attaaatgcc tattttgatt tagatacaga tggtgaaaac aggcaggcct
      781 tagttgacat agtggaaaaa gaatgtgtta tgatgcaaga agatgagccc tatatccttt
      841 atgaagtata tttaagggaa aaggaagaga tttataatat ttgtaaaaat ataattgact
      901 taactgaggc agatactgaa tgggagatct tgataaatga attggccttt ggaaccaagg
      961 acagaaaatt taataagatt tttggtgttt taccaaaaat caaggtggac attgccgata
     1021 caagtataca ggtagccggt tctatagata ggattgatgt tcataaggaa aatccaaaca
     1081 aactaaggat tgtagattac aaaactggta atagaagtta ttttgaaaga aaactgagag
     1141 aaaatatagg tagcaggggc aaacctgttt atgactatag tgaaactgaa aagtttcaac
     1201 attatattta tatgaaggcc ctggaggaga tattaaatac cagaagggat ttgttcccta
     1261 atccagaaat ccaaagcttt aaatatgtat ttgcagataa caggtctagc tcagtaataa
     1321 aaatagattt taatcaggac tttatggaag atatcgaaga aaggattaag tatctaataa
     1381 atatggacat cttattggca gataaagatg tggcatatga ctgggaaagg tatggcaaat
     1441 gtaactattg tgattataaa ttgatttgca tggctgataa aactttacag attaaggagg
     1501 tagaatagat ggaaaaacag gacttaatac aaagggaaat gataaaaaga gaagagataa
     1561 tgaacagctt taatacaaac tacctcctgg aggcaggggc tggggccgga aaaactacca
     1621 taatagtgga aagaattatt aaccaactta tacacactga tacagagccg gctaatatag
     1681 tagccattac ctttacaaag gcagcggcca ctgaattggc tgaacggatc caattgaaag
     1741 cattggatta cctgcaggat gttgaagatc acaggataag aagaaagttg gaatcaatag
     1801 atgaaatatt tacaggaacc atccactctt tttgtgattt aatgttgagg gaaatgccct
     1861 ttgaggcaaa tattactcca ggctatgaaa tagtggaaga ggaggcagac tttcataata
     1921 aggtttggta tgactttata agaaataagg aaggagacta caaggataag atagatcttt
     1981 tagaaaagtt ctccatagat tatagagaac tgagggatgg ggccttggtg gctatgagta
     2041 atccagatat agattttgta ggttatgaag attttaacct aacatatgct gagcttgaag
     2101 aagaatttta taaaataaaa aaggaatatc ataagttaaa tccttcatat ataaagagaa
     2161 gtccaaactt gagcaaatta ttaagagcca tattggaaga agataggagt ctagattatt
     2221 ttatagagga tatttttaag gagtgtccta aatatgaatt agatgaaaat actatttacg
     2281 aaaggataat ctataaaaag aatttagata atcctaacag gcaggaatat atggatttta
     2341 tatcaagagt tttccatctt tactctaccc taaataaatt gggctacaat acctgcactg
     2401 attttataaa tatggcagtg gactataagg aaaaaaacta cagaggtaaa ttgaccttta
     2461 atgaattgct gtacaaggcg tctcaactaa ttcagaactc ttctacagcc aggcagcact
     2521 ttaaagaaaa gtataagtac ttttatatag atgaattcca ggatacagat ccaatgcagg
     2581 ctgagttaat attttatttg actgatcagg aaaacgaagg agtcagaaaa agttgggtag
     2641 attgtgagcc aagggcagga tccttatttg ttgtaggaga tcctaaacaa tccatctata
     2701 ggtttagacg ggctgatatt acaatttata atcaagtaaa ggaaatctta gcccagcatg
     2761 gggaagtggt ttacctggat attaacttta gatcttcaga taatatttgt aattgggttc
     2821 aatctacctt taagaaagaa gaaggtggat ttgcctttgc aagtaaggca actgatactc
     2881 aggcaggatt tgaaaaaatt cttagcctat gggatgatga aataatagat aaaggccaga
     2941 catatggaag gaaaagaacc aatggaattt attactacga taatactagg attatggatg
     3001 aagatgtcca aatagatgaa gcagaatatg tggccaacct ggttgaaaat attatggaaa
     3061 gctattatct agttgaaaag gttagaaagt caaccatagg gatagaggaa aatgaatttt
     3121 ataaccaggt cagactggtt gagcctaaag attttatgat tttaacaaag actaataagg
     3181 aaacaggggt ttatctaagg gccctaaaag acaagggtat accggctctg ctggctggag
     3241 agaagacttt aggagatacg agagaggtat taaatctatt tatcctacta gatgcccttc
     3301 tagattatag ggatagtata aaggtagttg ccgccttgag gaattgcttt tatttagatt
     3361 tggccacaat cgatctattc atggaggata taaaggacag atccaatatc ctatatacag
     3421 atattaaaat aaaacactct aaggtggaaa aggccatatt atatatgagg gaaattgcca
     3481 gcccaatgag gaggctaagt cctattacct ttattgagaa acttataaaa aataggtttg
     3541 gaatctttaa tacagatagg gaatatagca cactagaaga aagggatgca gaatcagcct
     3601 tggctcaaac agtagaaatc ctaaaggcag caggctgtga tagtatatac caggttaggg
     3661 atgaattaga aaggttagtt aaagaacagg taaattatga gctacctata agcaccgaat
     3721 atgccaataa tgctataagg gtaatgaata ttcataaggc caagggcttg gaggctaata
     3781 tagtaattct ggtaggtaga gataaaaaag ataatttagg tagagattcc cattatgtag
     3841 aaaaggatat gtatggcaag attttaggtt atctagtgta taaaagggat tacaaactcc
     3901 tgggacctaa ggaaaaggaa aataaggaaa gagagaactt atttaagagg gctgaaaatg
     3961 aaaggctctt atatgtggct gccaccaggg caaagactag cttaatattg gccaagtcca
     4021 gtgatgaaga aaagtatttg tatcctttaa gccaaagggt agaggaagaa ctggttccta
     4081 caattaagaa ggttgagaat caagatatag gcctaggcaa ggggaaaagg gaagaattgg
     4141 aggaaacaaa agaattaagg gataaaaaaa taatccttga accatccttt ataagcctaa
     4201 gtccctctga ttttgttaaa ggtaagctag aggaagggaa taaatatagg gttggattaa
     4261 gtcctgtttt tacctccttt aaaaaagaaa atctaaagga tagggcttta ggacctagag
     4321 gtagtgtatt tggaaccatt atccataggg ccctagaagt tttaattaag ggtagcaggt
     4381 ccctaggcaa ggtagacaag gaaattatag aatatgccac caatatatca ataaatgaat
     4441 ctataaataa gctggagata aataaaacca atctaaatct attgtgtgct gatagtaaaa
     4501 aagtggagga gatgttaaat acaaaagaca aatttggaag cagaccagta agggaaaaaa
     4561 taaaaaaaga gataaggcct tatttggaaa caattttaat aaactttgcc aataataagg
     4621 aaataaagaa actgtttaag gaagctaggc aaatatttac tgaactaccc tttagtataa
     4681 atgttaaaga taatgtttta actaaattgg ccgatttttt aaacagggaa gaaatgcagt
     4741 catttatggc taaagacaag ctggttacag gtataataga tttagtaatt caatctgagg
     4801 ataatagctg gaccattatt gattataagt ctgatattat tacaaatgaa aatcaccatg
     4861 ctaggctggt aaagaggtat gagggccagc taagggccta taaaatattt ctagaagaaa
     4921 tactaaaaga agaaaaggtt caagtagata gactaatcct atattctacc tttaaggacc
     4981 agttgattta tttggaagag ccagctttag tttagggtgc tatttaaaaa gccacttaag
     5041 tggacaagga tatggctaga ctattggcag aagtctatca atatgatata ttttaagcac
     5101 atcttaagta aacattataa cttataggaa gaaataggat ctataggagt ggaaatattt
     5161 aaatagggta aaatcatgat agaatagttc atttatttga ctatagtgat ataatataag
     5221 tgaaaataaa tatggaggga ttatatgtta gtaacaggga aggaactact gttggatgct
     5281 cgggaaaata aatatgccat tggagcattt aatgtaaaca atatggaaat tgttcaagct
     5341 attattgaag ctgctgaaga attaaactca cctgttatac tacaggcaag ccagggcggg
     5401 ctaggctatg ctggagttga gtacatagct gccttaggaa agctagctgg caacaatgct
     5461 aaggttcctg tggtactaca cctggatcat gggacagact ttgatcaggt tatgcaatgt
     5521 attaggcatg gatttacatc tgtaatgata gatgggtcca ggttcccatt ggaagaaaac
     5581 attgcattta caaaaaaagt agtagaaata gcccattcag taggagtttc tgttgaggct
     5641 gagctaggta aaataggtgg aactgaggat catataacag ttgatgaaaa ggatgcaacc
     5701 ttaacagatc ctgaagaggc aaaaagattt gtggaagaga ctaatgtgga ctatttag
//
LOCUS       DATGSI010000378         1489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_258186, whole genome shotgun sequence.
ACCESSION   DATGSI010000378 DATGSI010000000
VERSION     DATGSI010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258186"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VK031_05850"
     CDS             <1..738
                     /locus_tag="VK031_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53474.1"
                     /translation="ISSMVRKDELSTEIIQNAESVVTAINSTDGVGKVKSVKVTVDDN
                     GLTVDDGAVIIRDSQNTAIITSQGLKVMFVYTSSGELNGWQKIGYQVQSGHPTQANAY
                     ITFIVPEKLQLTNAELVVHSLPSRWEGWSDHFGVPDGVYHARNMRLFKVSDGSNSVYD
                     YPAQSTPAVLMGITGRTDITNDVWGGQWTPSGINIQEKTGNVTNHIETGKYQTFGVAS
                     IDNLNTTNMRYMGGLKFTLVVEGYLRG"
     gene            743..988
                     /locus_tag="VK031_05855"
     CDS             743..988
                     /locus_tag="VK031_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53475.1"
                     /translation="MNLKEMLENARQKEKDIHLEYERRTEEYKRKVLENDEIRRQLEK
                     EEKELKEKIPKEPFEKDSEWGKVLEEIRVLERLVGDE"
     gene            988..>1489
                     /locus_tag="VK031_05860"
     CDS             988..>1489
                     /locus_tag="VK031_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53476.1"
                     /translation="MAELYKGIEGSPITYLTSDISASQTTISIDDDSALPDAPNICTI
                     GYGEHLETIKYETKSNGVLQNVTRGIEGTPRSWQAGTEVARFFTAYDHNKIIDKYNDL
                     TVNFNEHLADYESLRSKVAEREKKLFSDSSPAIFNLSAGSYYLLYAFVGLNTSQAMAT
                     YEIAMWS"
BASE COUNT          552 a          194 c          330 g          413 t
ORIGIN      
        1 atatcctcaa tggtcagaaa agacgaattg tctacagaaa taattcaaaa tgccgaatca
       61 gttgtaactg ctatcaattc aaccgatgga gtaggtaagg taaagtcagt caaggtgacc
      121 gttgatgata atggattaac agtagatgat ggggcagtaa taataagaga tagtcaaaat
      181 actgctatta taactagtca aggtttaaaa gtaatgtttg tttacacttc aagtggtgaa
      241 ttaaatggtt ggcagaaaat aggatatcaa gtgcaatctg gtcacccaac tcaagctaat
      301 gcttatatta cttttatagt tccagagaag ttacaattaa caaatgccga attagtagtt
      361 cactccttac cttcaaggtg ggaaggatgg agcgaccact ttggagttcc agatggtgtt
      421 tatcatgcta gaaatatgag acttttcaaa gttagtgatg gttctaatag tgtatatgac
      481 tacccagcac agagtactcc agcagttttg atgggaataa caggtaggac agatataact
      541 aatgatgtat ggggtgggca atggactcca tctggtataa atattcaaga aaaaacagga
      601 aatgtcacaa atcatattga aactggtaaa tatcaaacct ttggggtagc tagtatagat
      661 aatttaaata ctacaaatat gagatatatg ggtggtttaa aatttacatt agtcgtagaa
      721 ggatatttaa gggggtaata ttgtgaattt aaaagaaatg cttgaaaatg caaggcaaaa
      781 agaaaaagat attcacttgg aatatgaaag aaggacagag gaatataaga gaaaggtttt
      841 ggaaaatgat gaaattcgta ggcaactgga gaaggaggaa aaagaattaa aggaaaaaat
      901 acccaaggaa ccttttgaaa aggattcaga gtggggcaag gtattagagg aaataagggt
      961 cctagaaaga ttggtaggtg atgaataatg gcagaactat ataaaggtat agaaggcagt
     1021 cctataactt atctaacaag tgacatatca gctagtcaaa caaccatatc tatagatgat
     1081 gatagtgctt tacctgatgc tcctaatatt tgtacgattg ggtatgggga acatttagag
     1141 actattaaat atgagactaa gtccaatggt gtcctccaaa atgtaacaag gggtattgag
     1201 ggaacaccac gaagttggca agcaggtaca gaggtagcga ggttttttac agcctatgat
     1261 cataataaaa ttatagacaa atataatgat ctaactgtaa actttaatga acacttggca
     1321 gattatgaaa gtttaaggtc aaaagtagca gaaagagaaa agaagttgtt ttctgattca
     1381 tcccctgcaa tttttaattt atcagccggg agttattatt tgctttatgc ctttgttgga
     1441 ttaaatacat cccaagccat ggcaacatat gaaatagcta tgtggagta
//
LOCUS       DATGSI010000379         4824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_687625, whole genome shotgun sequence.
ACCESSION   DATGSI010000379 DATGSI010000000
VERSION     DATGSI010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687625"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1060)
                     /locus_tag="VK031_05865"
     CDS             complement(80..1060)
                     /locus_tag="VK031_05865"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HLS53477.1"
                     /translation="MANRLNIIQAINQALMNEMDLDETIVVYGEDVGVEGGVFRATVD
                     LQKTFGEERVFDSPLAESAIVGTAVGMAINGLKPVVELQFSGFSYPAFNQIVTHVARM
                     RNRSRGRYTLPMVIRSPYGGGIRALEHHSESTEALYAHIPGLKVVIPSTPYDAKGLLI
                     AAMRDPDPVLFWEPKKIYRAFRQEIPKEAYELPIGKAKLVEEGEDITIVSWGAMVREV
                     EKASQMVKEKGIQPEIIDLRTIAPMDRESFIESVKKTGRILVVHEAPKTLGLGAEIIS
                     IVNEKAFLYLEAPPTRLTGFDTTFPLAQGERHYIPSPERIAKTIEEVVKY"
     gene            complement(1062..2144)
                     /gene="pdhA"
                     /locus_tag="VK031_05870"
     CDS             complement(1062..2144)
                     /gene="pdhA"
                     /locus_tag="VK031_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HLS53478.1"
                     /translation="MRLEVYKPKNKEQYRILDAKGKIVREKDLPDFTDQELLYLYRTM
                     LFSRIIDERALSYQRQGRMLTYAPNIGQEATQVASAYAMGDEDWMASSFRELGAWLVR
                     GVPFRNIYLYWYGNEWGSHMPEGVNVLPVSIPIASQLLHAVGIGMANNIKGEDKVAVA
                     YVGDGGTSHGDFHEALNFAAVFKAPVVFIIQNNQYAISVPREKQTASETLAQKAKAYG
                     MPGILVDGNDIFAMYKSTKTAIERARKGEGPTLIEAYTYRLGAHTTSDDPSKYREDEE
                     VKEWEQKDPILRFRTYLKDKGLITDKWEEETKKELDNEVVSTFESIENKSATEIDDIF
                     KYHYETMPPQLEEQLEEYKAFLEGGN"
     gene            complement(2345..3061)
                     /locus_tag="VK031_05875"
     CDS             complement(2345..3061)
                     /locus_tag="VK031_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HLS53479.1"
                     /translation="MKNLILIVSLYGFAVGLLGTGLGGLISLFIKGTDKILSFLLGLT
                     AGFMLFIVTFHLLPEAFILSGILVTLLGIVFGIILVIFIEIYLDGLIKNPLTRSGLLL
                     GISIAVHNFPEGLALGSSFMTMSNFGPTLAVAMLLHNIPEGLSMAIPLKANRLSPLKI
                     LAYSALAGLPTGIGAFFGAYIGSISNTLISLCLSFAGGTMLYIIADEIIPGAKILHKG
                     RASSIGIVLGFALGIILYFK"
     gene            3201..4307
                     /locus_tag="VK031_05880"
     CDS             3201..4307
                     /locus_tag="VK031_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HLS53480.1"
                     /translation="MDRRLKYLWGGVLGSLILLLPLFMRNQVISIEEDEEISLRVDYE
                     LNQQLKELEDRLEIKRGVEVGLINGPKLVNEKDRRIVPPRLDKKVKMENDDVFKYAKR
                     DIGLKLDLASEFKKLTVIPKGEKVKVIGHLNGWDKLIYKDYVGYSLSEDLSEEKVAKK
                     EGSKAFRDQSKDRKKVKAKPKVEVKEKPKEKVEEVKIINGIILVNKEYPVPKNYYKGV
                     DPKAKNKVDKMLQAAREEAGFDLIIHSAYRSYNTQKTLFNNYVRRNGYDRASMYSAKP
                     GHSEHETGLAFDICDRARKYYLKQAFGDSEEGIWLRENAHRFGFILRYPKDKTHITGY
                     IYEPWHFRYVGEEHAKKIYEKDITLEEYLEPDRY"
     gene            4471..>4824
                     /locus_tag="VK031_05885"
     CDS             4471..>4824
                     /locus_tag="VK031_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HLS53481.1"
                     /translation="MLKVEKRNGSIVDFENEKIEIAITKAMKETEEGADEKLAKHIAE
                     EIYKEFKNEEVVHIEQIQDLVEIHLMQTRPDVARKYIIYREERARLREQGWDMTDLQR
                     DIYEKKYRYGGESFDE"
BASE COUNT         1574 a          860 c          977 g         1413 t
ORIGIN      
        1 tctggaaaac gaaagtggaa tttcatctta aaacctcctt atagaaaata tactctaaag
       61 tatattaata agctttaaat taatacttaa ctacttcttc tatggttttg gctatccttt
      121 ccgggcttgg gatatagtgt ctttctccct gggccagtgg gaaggtagtg tcaaaacctg
      181 ttaacctggt tggtggtgcc tccaggtata ggaaggcctt ttcgttaaca atggagataa
      241 tctctgctcc aagacctaaa gttttggggg cctcatgaac aaccaaaatc cggcctgttt
      301 ttttgacaga ttctataaag gactccctat ccataggggc tatggttctt aaatctataa
      361 tttctggttg gatacccttt tctttaacca tttgagaagc cttttctacc tcccttacca
      421 tagcacccca gctaactatg gttatatctt ctccttcttc caccaatttg gccttgccta
      481 taggtagctc ataggcctcc tttggaattt cttgcctaaa ggccctatag atttttttag
      541 gttcccagaa taggactggg tccggatccc gcatggcggc tatgagtaag cctttagcat
      601 catatggggt agaggggatt acaaccttta atcctggtat atgggcatac agtgcttctg
      661 tactttcaga gtggtgttcc agggccctga tacctccccc atagggtgaa cggataacca
      721 taggtagggt atacctacct cgtgatctat ttctcatcct agccacatgg gtaactattt
      781 ggttaaaggc aggataggag aaacctgaaa actgaagttc aaccacaggt ttcaagccat
      841 taatggccat accaacagca gttcccacaa tggcagattc ggccaagggg ctatcaaaaa
      901 ccctttcttc accaaatgtt ttctgtagat ctactgtggc cctgaaaacc ccgccttcca
      961 cacctacgtc ttcaccatag actactatag tttcatccaa gtccatttca ttcattaggg
     1021 cttggttaat agcttggata atatttaatc tgttagccat tttaattacc cccttctaaa
     1081 aaagccttat attcctctaa ttgttcttct aactgaggag gcatagtttc atagtggtac
     1141 ttaaatatat catcaatctc tgtggcagac ttattttcta tagactcaaa ggttgataca
     1201 acttcattgt ccaactcctt tttagtttct tcctcccact tatcagtaat aaggccctta
     1261 tctttcagat aggtcctaaa tcttaggatt ggatccttct gttcccattc cttaacctct
     1321 tcatcctccc tatatttgct gggatcatct gaggtggtat gggcgcctag cctataggtg
     1381 taggcttcaa ttagggttgg gccttcacct tttctagctc tttctatggc tgtctttgtt
     1441 gacttgtaca tggcaaatat atcattgcca tctactagga tgccaggcat accataggcc
     1501 ttagcttttt gggccagagt ttcgctggca gtttgttttt cccttgggac agatatggca
     1561 tattggttat tttgaattat aaagactact ggtgccttaa agactgctgc aaagtttaaa
     1621 gcttcatgga agtctccatg ggaggttcca ccgtcaccaa cataggcaac tgccacctta
     1681 tcttcaccct tgatattgtt ggccattcct atacctacag catgtaatag ttgggatgca
     1741 ataggaatag aaactggcaa aacatttaca ccttcaggca tatggctgcc ccattcatta
     1801 ccataccagt agagataaat atttctgaag ggaactcccc ttactaacca tgcacctagc
     1861 tccctgaaac tggaggccat ccagtcttca tcgcccatgg cataggcgct ggcaacctgg
     1921 gtagcctctt gacctatatt tggagcatag gtaagcattc taccctgcct ttgataggat
     1981 agggcccttt catctataat cctgctaaat agcatagtcc tatataaata taagagttct
     2041 tgatctgtaa aatctggaag atccttttcc cttacaatct tccccttagc atcaagaata
     2101 cgatattgct ccttgttctt tggtttatat acttccagcc gcatatataa aacctcccgt
     2161 aaatataaga tgtattgata atgattatca tttaccatat atattcccaa tagaaaagat
     2221 aataaactgg aaaaaggata gctgaaataa gtctctatat tcagaaaagt aggcctattt
     2281 cttaaagctt gtccaaaagt tatacctggc aggctagatt gtaaaaatga aaataaactt
     2341 ggctttattt aaaatacaaa attataccta atgcaaaacc taaaactata cctattgatg
     2401 aggccctacc tttatgaagg attttggccc ccgggattat ttcatctgca ataatatata
     2461 gcatggtccc accggcaaaa gataagcata gggagattag ggtgtttgaa attgaaccta
     2521 tataagcccc gaaaaaggca ccaattccag taggtaggcc tgccagggcc gaataggcta
     2581 ggatttttaa aggagataat ctattagctt ttaagggtat ggccatggat aagccttctg
     2641 gaatattatg taaaagcatg gcaacagcca aggtggggcc aaagtttgac atggtcataa
     2701 agctggatcc tagtgccaat ccttcaggga aattatggac tgctatactt atacctaata
     2761 ggaggccact tctagttaaa ggatttttta tcagcccatc caggtaaatt tctataaata
     2821 taaccaagat tattccaaag acaatgccaa ggagagttac taggattcca cttagaataa
     2881 aggcttcagg aagcagatgg aaggtaacta taaataacat aaatccagct gtaaggccca
     2941 atagaaagct taagatctta tcagtacctt ttataaatag actgattagc ccacctaggc
     3001 cagttcctaa aagacctact gcaaaaccat aaaggctaac aatcaagatt aaatttttca
     3061 taaaattcac cccttcacac actaatatta ttcttactag gtgaaataaa gaacaaaatc
     3121 ttaaaaagtg atgtttctac tattgtaaag tgtatgtgtt atgtaataat atatatacat
     3181 agctgtatgg gggggatttt ttggatagaa ggcttaagta tttatgggga ggagtattag
     3241 gctctttaat acttctatta cctttgttta tgaggaatca ggtaattagt attgaggaag
     3301 atgaagagat aagtctgagg gtggattatg agttaaacca gcagcttaag gagctggagg
     3361 ataggctaga gataaaaaga ggagttgaag ttggtctaat aaatgggcct aaattagtaa
     3421 atgaaaagga tagaagaata gtacccccta gattagacaa aaaggtaaaa atggagaatg
     3481 atgatgtttt taaatatgct aagagggata taggcttaaa gttagatctg gcttctgaat
     3541 ttaagaaact aactgtaatc cctaagggag aaaaggtaaa ggtaataggt catcttaatg
     3601 gatgggacaa gctaatatat aaggattatg taggttattc tttaagcgaa gatttaagtg
     3661 aagagaaagt agcaaaaaaa gaggggtcaa aagcttttag agatcaatcc aaggatagga
     3721 agaaagttaa agcaaagcct aaagtagaag ttaaagaaaa acctaaggaa aaggttgagg
     3781 aagttaaaat aataaatggg ataattttag taaataagga ataccctgtt ccaaagaact
     3841 actacaaggg ggtagaccct aaggctaaaa ataaggtgga taaaatgttg caggcagcca
     3901 gggaagaggc tggatttgat ctaataattc attcggctta tagaagctat aatacgcaga
     3961 aaaccttgtt taataattat gttagaagaa acggatatga tagggcctct atgtactcag
     4021 ccaaaccagg tcattctgag catgaaacag ggctggcctt tgatatatgt gatagggcta
     4081 gaaaatacta cttaaaacaa gcctttggtg atagtgaaga aggcatatgg ctaagggaaa
     4141 atgcccatag gtttggattt atcttaagat atccaaagga taaaactcat attactggct
     4201 atatttatga gccttggcac tttaggtatg taggagaaga gcatgcaaag aaaatatacg
     4261 aaaaagatat taccttagaa gaatatttgg aaccagatag atattaaact ggactacggt
     4321 ccagttttta aatttgtaaa ataagttaaa aagatattga aaaaacaact aacttgtgct
     4381 aaggtatata tctagtgggt atacaatata ccatacacta catatagggt ctcgccctat
     4441 attttattat cttataatgg agggacaaaa atgctaaagg ttgaaaaacg gaatggtagc
     4501 atagttgatt ttgaaaatga aaaaattgaa atagcaataa ctaaggctat gaaggaaaca
     4561 gaggaagggg cagatgaaaa actagccaag catatagcag aggagattta taaggaattt
     4621 aaaaatgaag aagtagttca tatagaacaa atccaggatt tggtagagat tcatttaatg
     4681 cagactagac ctgatgtggc aagaaaatat attatttaca gggaagaaag ggctagatta
     4741 agagaacagg gctgggatat gacagacctt caaagggata tatatgaaaa gaagtataga
     4801 tatggtggag aatcctttga tgag
//
LOCUS       DATGSI010000380         1219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_169404, whole genome shotgun sequence.
ACCESSION   DATGSI010000380 DATGSI010000000
VERSION     DATGSI010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169404"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /gene="iscB"
                     /locus_tag="VK031_05890"
     CDS             complement(<1..805)
                     /gene="iscB"
                     /locus_tag="VK031_05890"
                     /inference="COORDINATES: protein motif:HMM:NF040563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease IscB"
                     /protein_id="HLS53482.1"
                     /translation="MLVFVINKHGERLMPCKPSKARKLLRDRKAKIIKHNPFTIQLLY
                     GSSGYKQPINLGIDLGAKHIGVAITSKNNVLAKGEIELRQDIKSSLETRKIYRRSRRN
                     RKTRYRPTRFLNRVSSKKNGWLPPSILSRIDNTFMWIDKFTNLLPNPKVSIEVGKFDV
                     AKMINPKIEGVDYQKGQTYGYHDTRYFVFARDNYTCQVCKKKDKILQIHHIKYVSKGG
                     SNRADNLITVCTDCHTSKNHKEGGVLYDWMVKNKKVKTYKEATFMNTIRQ"
     gene            complement(1034..>1219)
                     /locus_tag="VK031_05895"
     CDS             complement(1034..>1219)
                     /locus_tag="VK031_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53483.1"
                     /translation="DMIDGVVYLVDLIRYKVYPINSLKLKSKMVTMSMYETTYGSHLN
                     ESCRKAKEIVNNSPLRV"
BASE COUNT          358 a          227 c          154 g          480 t
ORIGIN      
        1 tttgtctaat ggtgttcata aaagtagctt ctttatacgt ctttaccttc ttattcttaa
       61 ccatccaatc atatagaaca ccaccttctt tgtggttttt gctggtatgg caatcagtac
      121 aaactgtaat tagattatct gctctattac ttccgccttt tgaaacatat ttgatatgat
      181 gtatttgtaa tattttatcc ttctttttac aaacttgaca agtgtaatta tctctagcaa
      241 atacaaaata cctagtgtcg tgatacccgt atgtttgccc tttttgataa tcaacacctt
      301 ctatttttgg attaatcatt ttagccacat caaatttacc aacttcaata gatacctttg
      361 gattaggaag taaattagta aatttatcaa tccacataaa agtattatct atcctactta
      421 atatgctagg tggtaaccac ccattcttct tggaactaac tctattaaga aatctagtgg
      481 gtctatacct tgtttttcta tttcgtctgc ttcttctata tatttttcta gtttctagtg
      541 atgattttat atcttgtctt agttctatct caccttttgc taacacatta tttttacttg
      601 tgatagctac tcctatatgc tttgcaccta aatctatacc caaattaata ggttgcttat
      661 aaccactact cccatacaat aactgtatag taaaagggtt atgttttata attttagctt
      721 ttctatctct taataatttt cttgctttac ttggtttaca tggcataagt ctttcaccat
      781 gtttattaat aacgaacact aacataaagt gttcctcctt tcagagttat tacccttcga
      841 caatgttatt atggcttgta tgtgagaaca tactcattag gttcactgtt cctactcatc
      901 agaactgttt agagagccta cacagagctt agaactaggg tatcattcta aggtgtgata
      961 actataataa cgtagtaaat gaatacttag tctagtcaat catagctttt acaagctccc
     1021 acttcaagaa tcattagact ctaagtggtg agttattgac tatctctttg gcttttctac
     1081 atgattcatt taagtggctt ccataagtag tttcatacat actcattgta accatcttag
     1141 atttaagttt taatgagtta ataggatata ctttatatct tatcaaatct actaaataaa
     1201 caactccatc aatcatatc
//
LOCUS       DATGSI010000381         1653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_258368, whole genome shotgun sequence.
ACCESSION   DATGSI010000381 DATGSI010000000
VERSION     DATGSI010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258368"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VK031_05900"
     CDS             <1..511
                     /locus_tag="VK031_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLS53484.1"
                     /translation="KVKATFFVLGKSAQVQKNTLLRAYKEGHQIGNHSWDHKDLKKLS
                     EEEIISQINKTNEVISNITGEEVRVMRPPYGSFNDIVKSKVNMPIILWNLDPLDWKDR
                     DASIVAKRMSEGDRNGIVLAHDIHKSTVEAMPEVISNLKAKGYHLVTIDNLFSGKELV
                     DGKIYNRRQ"
     gene            817..1431
                     /locus_tag="VK031_05905"
     CDS             817..1431
                     /locus_tag="VK031_05905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002075262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HLS53485.1"
                     /translation="MNKNNFFDSPFVGKQLKEQITNPNIIVGDYSYYSGYYSGHSFDD
                     CARYLMADREDVDKLRIGNFCSIADGVKFMMAGNQGHRYDWVSSFPFFYQGFEEAKDA
                     YVKVGDTIIGNDVWIGAEAIIMPGIRVGDGAIIATGAIVTKDVEAYTIVGGNPAKPIK
                     KRFSEKEITMLVELKWWNWSIEKIKLNMDLICSKNITELYNKNI"
BASE COUNT          598 a          215 c          350 g          490 t
ORIGIN      
        1 taaggttaag gctacgttct ttgtattggg taaatcggcc caagtgcaga agaatacgct
       61 tttaagagct tataaagaag ggcatcagat aggcaaccat agttgggatc ataaagactt
      121 aaagaaactg tctgaggaag aaattattag tcagataaac aaaaccaatg aagtaatctc
      181 caatattact ggagaggaag ttcgtgttat gcgaccgcct tatggatcgt ttaatgatat
      241 agtaaaaagc aaagtgaata tgcctattat tctatggaat ttagatcctt tagattggaa
      301 ggatcgtgat gccagtattg tagcaaagag aatgagcgaa ggggatagga atgggattgt
      361 attggcacat gacattcata aatctaccgt agaagccatg ccagaagtaa tttctaatct
      421 taaggcaaaa ggctatcact tggtaactat agacaattta ttctcgggta aagagcttgt
      481 tgacggaaaa atatataata gaagacagta aatatttaag ctagataagg agtgatagag
      541 cagaaaacat acttaaaaga ggaacctaaa atctttaagt taatctacat aataggtata
      601 gtttgtctca tagcctactg tgttttagat tggattttta atatgccaat taaattttta
      661 caaatcctag gtttcttggg tatgatattt ttctttttaa gaatggtata caaacaatgg
      721 agaagaaaag ggaataacta ggttgtggtg catttaaaaa aaaccagcta cttctattca
      781 atcttgtaaa attcaaattg ttcaagtaag aaaaatatga ataaaaataa tttttttgat
      841 tccccatttg tgggcaaaca acttaaagag caaattacta atccaaatat aatagtaggc
      901 gattacagtt attactctgg ttactatagc ggacattctt ttgatgattg tgctagatac
      961 ctaatggctg acagagaaga tgtagataaa ttgcgaattg gtaatttttg ctccatcgct
     1021 gatggtgtca aatttatgat ggcaggtaac caaggtcata gatatgactg ggtaagctcc
     1081 tttccatttt tttatcaagg atttgaagag gcgaaagacg cctatgtaaa agttggagat
     1141 actattatcg gcaacgatgt ttggataggt gcagaagcga ttataatgcc aggaattagg
     1201 gtaggggatg gtgcaataat tgctacagga gctattgtaa caaaagatgt agaagcttat
     1261 actattgttg gaggaaatcc agctaaacca attaaaaaaa gattctctga aaaagaaatt
     1321 actatgttag tagagcttaa atggtggaat tggagtatag aaaagataaa attaaatatg
     1381 gatttgattt gctcaaaaaa tattacggaa ttatataaca aaaatatata gcggtcacgc
     1441 cacctggcat atgtaaacta agcgaaacag taataaatca gagcttttag cttgtttcaa
     1501 acttgatgat taaccgaaga agtgcaacga gaagctattc ttcagtttat gataagcgag
     1561 gctataaagt ctgccgagat agaagggggg agtatcacat taagtgtgta agttaagatt
     1621 tagggggtaa agctttaaaa ctttaccccc tat
//
LOCUS       DATGSI010000382         6267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_404292, whole genome shotgun sequence.
ACCESSION   DATGSI010000382 DATGSI010000000
VERSION     DATGSI010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404292"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1313
                     /locus_tag="VK031_05910"
     CDS             183..1313
                     /locus_tag="VK031_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HLS53486.1"
                     /translation="MKNDNFVLEQYLDELEELVNIDSGSTYVEGVNKIADIFKEKYEN
                     LGFLVKEHNFSKDAGSCLEIRNKDGKDIDILIIGHMDTVFKKGTAAERPFTIKGDRAY
                     GPGVADMKSGLLSTYYSMKLLNKEVLDKLNICIFHNSDEEISSIYSQEKIKELARKSR
                     YAFVMEPGRSDGSMVTERKGLAKYFIDFHGVAAHAGVEPEKGKSAIHELGNWIVNLVK
                     LNDYESGTSVNIGVVEGGTVPNVVADKAHGQIDIRFKSDNVIDDIEKAMQELKENPLV
                     EGIDIKVERKGYRPPMNPNEDVYKLCRQIEKIGEELGIEIKWTATGGGSDANFTSAEG
                     VPSIDSMGPIGGGAHGVDEYIEIDSIEPRIKLFTRLLENLAD"
     gene            1432..2832
                     /locus_tag="VK031_05915"
     CDS             1432..2832
                     /locus_tag="VK031_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLS53487.1"
                     /translation="MTYDLIIRNGTVVDGETTEKAKDIGVKSGKIIDVSDELDPSLAS
                     ETIDATGKWVMPGLIDTHVHVVREDCTDEGFRMLLRAGVTTAVDFRGPIELLLNNFKQ
                     SQFRPNIALLNGVYPGNGFKELDVSYKEIENFVEYSLENGSIGIKVLGGHFPVTNETL
                     GRAIEVSNKMKSYIAIHCGNTQNGSNIKGFLDAVKMAQGGRLHIAHVNSYCRGLVYNE
                     LEETKIALENLKANRNIISESYLSGINGTSGNIDKDNRPVSHVTRNCLKMKNYDISKK
                     GLEKAILNGDCAVYYKTGDEMVLHYGQEAVDYWESVNYKANLCFDVNPVVPRIACATS
                     KNDKGEFIVTALSTDGGAIPRNSLLDKGLLLVEFGALTISDFCLKTSLSPAQMLGLST
                     KGRIKKNFDADLVVVDKNSRKPIYTIIGGSIAAKGDKLMESKGKLLILEQGEKNIKRK
                     GLDYQVIDLQTSLLYR"
     gene            3127..3591
                     /locus_tag="VK031_05920"
     CDS             3127..3591
                     /locus_tag="VK031_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HLS53488.1"
                     /translation="MERRVVMIEKNLIKLDLDYETRADVITRLGQLLHEKDYVKKEFI
                     QAVLDREEKHPTGIPAEPAIALPHTSSDLVKRAAIAVAILDKPVEFYQMGGSGERLNV
                     KIVFMLAIDDPEKHLNYLQRIMDILEDDEALKKLENSTTVEDAYKVLSYINS"
     gene            3630..5747
                     /locus_tag="VK031_05925"
     CDS             3630..5747
                     /locus_tag="VK031_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HLS53489.1"
                     /translation="MNELVLYKRASLILLTLVNTDRPITISYLQSLTKASDRSIRYDL
                     DNIDQFLKSKGLKPIERVPGTGIFFVGDKDERAQVKRLLKDVDIYNYVPSPKERKVLI
                     ILEFIMAPNYITTDYLAEILDVSRNTINSDLKGVKEELDKYKLKLKFKQRYGLILEGA
                     EANIRKLLVEMIIKHLFNLSANGSKISEGISNLVSNRIKELVIGEIDIRFLEGIVNGI
                     EEELGFDYSDLAYENILTHLIVSIVRTKAGNYISINSFNIEVIKETREFKIIKNIKVL
                     FEDLYKITVTDSELAFITTSLLGGNFSTNQNITKGDWVFIQIMVRNFIEEVNSKLMVD
                     ISKDWRLYNSLLAHIKPMLNRIRYGIRLENPLIKSIKKNYKGLYKIVEESVYELEQFI
                     GKEMSQDEIGYLTLHFGGAIERTKLSSSYKPRVLIVCETGLGTSELLMIQIQSLFDLN
                     IVGVTSRRKLEETLKDKHIDAIISTIAIKVQEDIEVLEVRAILSDEDIRNLNSLFYKF
                     RKTNIEIKGLISLVERYATIHDYKNLEKDLHNLFNIKSMWDSNIGDRPNLLDTISRNT
                     IQLQSDITDWEEAVRLGGELLLKAGAVEERYIEAMVSTVKKYGGYIIVSPKAAMPHAK
                     PRDGAKKTKMSMVTFKESIRFENAEDIDIRLIICLSTTNSTSHLKALSQLMVLLEDEE
                     AVEALINADSKNEVINIIAKYSY"
     gene            5790..6092
                     /locus_tag="VK031_05930"
     CDS             5790..6092
                     /locus_tag="VK031_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53490.1"
                     /translation="MAKKRVIVCCADAVATSTLVAGKLRRLFQKEGLDVEIKTCSNLN
                     VRNMAKSFKPDVIVSNVGVQIKTDDNIPVLDGTTLLTGRGSDEVEKEIVSILTEED"
BASE COUNT         2338 a          781 c         1362 g         1786 t
ORIGIN      
        1 ttggctgcag gtatagctag gagtgtatct ccaataactt ctgtaatagt tgcagtttca
       61 ggaatatctg aagaatcaac tgtagatgtt gtaaaacgga ccgctatccc tatggcagga
      121 gctttaatag ccatggtaat atcatccttt atctttatat agaaatttaa ggaggaatat
      181 atgtgaaaaa tgacaacttt gtgttggaac aatatttaga tgaattagag gaactagtca
      241 atatagatag tggaagtacc tatgttgaag gtgtaaataa aatagcagat attttcaaag
      301 agaagtatga aaacctagga tttttggtaa aagaacataa tttcagtaag gatgcaggaa
      361 gctgtctgga gataagaaat aaagatggca aagatattga tatccttatc ataggtcata
      421 tggacacagt ttttaagaaa ggaactgctg cagagagacc ttttactatt aagggagaca
      481 gggcttatgg tcctggtgtg gccgatatga aatcaggctt gctttctacc tattattcca
      541 tgaaattatt gaacaaggaa gttttagata aattaaatat atgtattttc cataatagtg
      601 atgaggaaat tagttcgata tattcacagg agaagataaa agaactagcc agaaaaagca
      661 ggtacgcctt tgttatggaa ccaggtagat ctgatggatc catggttaca gaaagaaagg
      721 ggctagctaa atattttatt gatttccatg gggtggctgc tcatgctggt gttgaaccag
      781 aaaagggtaa aagtgcaata catgagctag gaaactggat tgttaattta gttaaactta
      841 atgattatga gtctggaaca tcagtaaata ttggagtagt agaaggtgga actgttccaa
      901 atgttgtagc agataaggca catgggcaga ttgatataag atttaaatca gacaatgtaa
      961 ttgatgacat tgaaaaggca atgcaggagc tgaaggaaaa tccacttgtt gaaggaatag
     1021 atattaaagt ggaaagaaaa gggtatagac cacctatgaa tccaaatgaa gatgtatata
     1081 aattgtgtag gcaaatagag aaaatagggg aagaactagg tattgaaatt aagtggactg
     1141 caacaggcgg tgggtcagac gctaacttta cttcagcaga gggagtgcca tccatagact
     1201 ccatggggcc aattggagga ggagcacatg gagttgatga gtatattgaa atcgattcta
     1261 ttgaaccaag gattaaatta tttacaagat tattagaaaa tttagcagac taggattcag
     1321 aagggctctg atattctttt aataggattg gagccctttt ttaataagat atttaaaaga
     1381 gtagattggc ctatttatta atagggaaat tacctcaaag ggaggaattc tatgacatat
     1441 gatctaataa taagaaatgg tacagttgtg gatggggaaa ctacagaaaa agcaaaagat
     1501 ataggtgtaa aatctgggaa gattattgat gtttcagatg agttagatcc cagtttggca
     1561 tccgaaacaa ttgatgcaac aggaaagtgg gtaatgcctg ggttgataga tacccacgtc
     1621 catgtagtaa gagaggattg tacagatgaa ggatttagaa tgctattgag agcaggagtg
     1681 actacagcag ttgacttcag ggggccaata gagttattgc ttaataattt taaacagagt
     1741 caatttcgtc ctaatattgc tcttttaaat ggagtgtacc caggtaatgg atttaaggaa
     1801 ttggatgtat catataagga gatagagaat tttgttgaat atagtttaga aaatggctcc
     1861 atagggataa aagttctggg aggacatttt cctgtaacta atgagacatt gggaagggct
     1921 atagaagtgt ccaacaagat gaagtcctat attgcaattc actgtggtaa tactcaaaat
     1981 ggcagcaaca taaaaggatt tctggatgca gttaaaatgg cacagggagg caggttacat
     2041 attgctcatg taaattcata ttgtaggggg ctagtatata atgaattaga ggaaaccaaa
     2101 atagcacttg agaacttaaa ggcaaatagg aatattataa gtgagtcata tttatcagga
     2161 ataaatggaa catcagggaa tatagataag gataataggc cagtaagcca tgtgacccgg
     2221 aattgtttga agatgaaaaa ttatgatatt agcaaaaaag gattggaaaa agctatacta
     2281 aatggagatt gtgctgttta ttataaaact ggagatgaaa tggtgcttca ttatgggcaa
     2341 gaagccgtag attattggga atcagttaat tataaggcaa atctttgttt tgatgttaat
     2401 ccggtggtgc caagaattgc ctgtgcaaca agcaagaacg ataaggggga atttatagta
     2461 acagccttga gcactgacgg tggagcaata ccaaggaaca gtttattaga taagggttta
     2521 ttattggttg aatttggtgc actaactatt agtgactttt gtttaaaaac aagtcttagc
     2581 ccggctcaaa tgttaggttt aagcaccaaa ggaagaataa agaagaattt tgatgccgat
     2641 cttgtggtag tagataaaaa tagtagaaag cctatatata caattattgg agggtctatt
     2701 gcagctaagg gagataaact gatggagtcc aagggaaaac tgttgatatt ggaacaggga
     2761 gaaaagaata taaaaagaaa aggattagat tatcaagtaa tagacctaca aacaagtctt
     2821 ttatatagat aaataattgt ttgcagctaa acattcagtt ataaaaagat tttgattagt
     2881 tttggaagtc aagtagattt ctaattaaaa aactctttca aggttaagtg accttggaag
     2941 agtttttctt aattaaaaga ttgataatac gttactacac aaaagttgcc acaataaatg
     3001 tgggtatatt tgttatttaa tcaattgcct gtgggtgcta tacttttact agcagagaaa
     3061 aacatagctt atagtttaaa ttaaatttgg ataaggcaat taatttatct attattgaca
     3121 aactatatgg aaagaagggt agttatgatt gaaaagaatt taataaaact agatctagat
     3181 tatgaaacaa gagctgacgt aattactaga ttaggacaac tattacatga aaaggactat
     3241 gtaaagaaag aatttatcca agcagtttta gacagagagg aaaagcatcc tactggaata
     3301 ccggcagaac ctgccatagc cctaccccat acttcaagcg atttggtaaa aagggcagca
     3361 attgcagtgg ccatattaga taaacctgta gaattctatc aaatgggtgg ctcaggtgaa
     3421 aggcttaatg tgaagatagt ttttatgttg gccatagatg atccagaaaa acatttgaac
     3481 tatttacaaa gaattatgga tatattagaa gatgatgagg cgctgaaaaa gttagaaaat
     3541 tctactacag ttgaagatgc atataaagtg ttaagctata ttaattccta attggaagga
     3601 tgaaattcta acctttcaaa aaaagagagg tgaatgaatt ggttttatat aaaagggctt
     3661 cacttatact attaactttg gtaaatactg atagaccaat tacaatttca tatttacagt
     3721 cgttaacaaa ggccagtgat agaagtatta gatatgatct ggataatata gatcaatttc
     3781 tgaaaagtaa gggcctaaaa cctatagaga gggtacctgg cacagggatc ttctttgtag
     3841 gagataagga tgaaagagct caggttaaaa gattgttaaa agatgttgat atctacaact
     3901 atgttccttc tcctaaggag agaaaggtat taatcatatt agaatttatt atggctccaa
     3961 attatataac aacagactac cttgcagaaa ttttagatgt aagtagaaat acaataaaca
     4021 gtgacttaaa aggtgtaaaa gaagaactag ataaatacaa gctaaaatta aaattcaaac
     4081 aaaggtatgg cctgatccta gaaggagctg aggccaatat taggaaacta ttagtagaga
     4141 tgattataaa acatttattt aatctatctg caaacggttc taagatttca gaaggtataa
     4201 gcaatttagt aagcaatagg ataaaggagt tagttatagg agaaattgat attagattct
     4261 tagagggaat agtaaatgga atcgaagaag aactaggatt tgattattct gatctagcct
     4321 atgaaaatat tttaacccac ctgatagtat caattgtcag aacaaaagca ggcaactata
     4381 tttccatcaa ttcatttaat attgaggtta ttaaagaaac aagagaattt aagattataa
     4441 aaaacattaa ggtcctattt gaagatttat ataaaataac agtaaccgat tctgaactag
     4501 cctttataac aacaagccta ttgggaggca atttctctac taatcaaaac ataactaaag
     4561 gcgactgggt ctttatacaa attatggtga gaaactttat tgaagaggtt aactcaaagc
     4621 taatggtaga tattagcaaa gattggagac tgtataatag cctgctggcc catataaagc
     4681 ctatgttaaa ccgaataaga tatggtatta ggctggaaaa tcctctgatt aaaagtatca
     4741 agaaaaatta taagggccta tataagatag ttgaagaaag tgtatatgaa ttggagcaat
     4801 ttataggtaa agaaatgtcc caagatgaaa tagggtatct aacccttcat tttggagggg
     4861 ctatagaaag gacaaagctt agcagctcct acaaaccgag agttttaatt gtatgtgaaa
     4921 caggcctagg gacatcagaa ctattgatga tacagattca atccctcttt gatttaaata
     4981 tagtaggagt gacatcgcga agaaagctgg aggagacttt aaaagataaa catatagatg
     5041 ccataataag caccatagct attaaggttc aggaagatat tgaagtctta gaagttagag
     5101 caattctgtc agatgaggat attagaaatt taaattccct attctacaaa ttcagaaaaa
     5161 caaatattga aattaaaggt ctaatatccc tggtggaaag atatgctact atccatgatt
     5221 ataagaattt ggaaaaagat ttacacaatc tatttaacat aaaatccatg tgggattcaa
     5281 atataggtga caggcctaat ctattagata ccatatctag aaatactatt cagcttcaat
     5341 cagatataac tgattgggaa gaagcagtaa gattaggtgg ggagttattg ctaaaagctg
     5401 gtgctgtgga agaaaggtat atagaggcaa tggtatcaac agtgaaaaaa tatggtggct
     5461 atataattgt atctcctaag gcagccatgc cccatgcaaa gcccagggat ggggctaaaa
     5521 aaacaaaaat gagtatggta accttcaagg aaagcataag gtttgaaaat gcggaagata
     5581 tagatattag gttaatcatt tgcctaagca ccaccaatag caccagtcat ttgaaggcct
     5641 tgtctcagct tatggttttg ttagaagatg aggaggctgt agaagcctta attaatgcag
     5701 atagcaaaaa tgaagtaatc aatattattg ccaaatatag ttattaaaaa tttagtaaat
     5761 aaatttaaga aagaagggag gtttaataaa tggctaaaaa aagagtaata gtgtgttgtg
     5821 cagatgcagt agctacatct actttagtgg caggcaagct aagaaggctt tttcaaaagg
     5881 aaggcctaga tgtggaaatt aaaacttgct ccaacttgaa cgttagaaat atggcaaaga
     5941 gctttaagcc agatgtaata gtatctaatg taggtgtaca aattaaaact gatgacaaca
     6001 ttccagtatt agatggaaca acgcttttaa caggtagagg tagtgatgaa gtagagaagg
     6061 aaatagtcag catactaact gaagaagact agcaacatgg gagggaaaat tatggacaca
     6121 attgttaagg tctttacctt tattaatgat atgggagcat ccatatctat gccaatcctg
     6181 atgacaatta ttggactggc tgtaggatta cgatttcgtg atgcaatcag atctggaatt
     6241 atgtatggta ttggttttga aggaatt
//
LOCUS       DATGSI010000383         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_307597, whole genome shotgun sequence.
ACCESSION   DATGSI010000383 DATGSI010000000
VERSION     DATGSI010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307597"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..597
                     /locus_tag="VK031_05935"
     CDS             127..597
                     /locus_tag="VK031_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53491.1"
                     /translation="MKNIIKTKLCPILSAKIFAQAIEDVPVIIQLDDDTIKENKVLPL
                     DRKLLDLPIINSYAGYMSTNDIYKIVNIESIKYISFDSKVFTLLDIAKSATDSYFPQK
                     EGYLGEGITVAIVDTGVVKHSDLVKPENRIVGFKDLINNKKSPYDDNGHGTHVG"
     gene            complement(636..1247)
                     /locus_tag="VK031_05940"
     CDS             complement(636..1247)
                     /locus_tag="VK031_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53492.1"
                     /translation="MVRSTTRTRNNQYRDLTYAYLRCKNNALGGDLICKKVNRINYTH
                     LYNYVEAEFLKIMAIYKNHEEATELTTKQIKRINYTKELRKLSTSLQGINKSLNEKNR
                     ILTNLYIDKSKGTISDEDYLIISSNLQGEREQLELRKKSLKDEIEKIERLKKEKADIE
                     KVVKAYIKEHKLTHEIVNEIIDYIEIGSPEDGQGRTINIYWKL"
     gene            complement(1199..1351)
                     /locus_tag="VK031_05945"
     CDS             complement(1199..1351)
                     /locus_tag="VK031_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53493.1"
                     /translation="MRYKDSWTLKDGIKKEKERPIYLLQKLGAYIVVVLWYEAQQEPE
                     TINIGI"
BASE COUNT          465 a          264 c          225 g          631 t
ORIGIN      
        1 attattggag aattatataa atttgtacca aacaaataaa gggcttgccc tttatttgtt
       61 tttgtcatct tcctagacac atatttttta ctgccagcat aaaataaagg aaggaaggtg
      121 gtaatcttga aaaatattat aaaaactaag ctctgtccca tattaagtgc taaaattttt
      181 gcccaagcca ttgaagatgt tcctgttatc attcaactgg atgatgatac tataaaagaa
      241 aataaagtct tacccctgga taggaaattg ttagatttgc caattataaa cagctatgca
      301 ggttatatgt ctaccaatga tatttataaa atagtaaata ttgaaagcat aaaatatatt
      361 agctttgatt ctaaggtttt taccttgctt gatatagcca aatctgctac tgatagctac
      421 tttcctcaaa aggaaggcta cctaggtgaa ggcataactg tggctattgt tgatacagga
      481 gtggttaaac atagcgattt ggttaaacct gaaaatagga tagttggatt taaggatctg
      541 atcaataata aaaagtctcc ctatgacgat aatggccatg gaacccatgt ggggtgaaca
      601 gtaaaccaaa aatttaatag ttataaattt actacctata gtttccagta tatattaatt
      661 gttctaccct gtccatcttc aggagatcct atctctatat agtctataat ttcgttaact
      721 atttcatggg ttaatttgtg ttccttaatg tatgccttaa ccactttttc tatatctgct
      781 ttttctttct ttaatctttc tattttttca atttcatctt ttaaggactt tttccttaac
      841 tctagttgtt ccctttctcc ctgtaagttg gaagatataa ttagataatc ctcatctgaa
      901 atagtaccct ttgacttatc tatatataaa ttggtaagta ttctattctt ttcattaagg
      961 ctcttattaa taccttgtaa tgaagttgaa agctttctta actcctttgt atagtttatc
     1021 ctttttattt gcttagtagt taattctgtt gcttcttcat gatttttata tatagccatt
     1081 atctttaaaa attcagcttc aacataatta tataggtggg tataatttat tctattaact
     1141 tttttacata tcaaatctcc acctagtgca ttatttttac atcttaaata agcatatgtt
     1201 aaatccctat attgattgtt tcgggttctt gttgtgcttc gtaccataga accaccacaa
     1261 tgtaggcacc taacttttgt agcaaatata tgggtctttc cttttctttt tttattccgt
     1321 cttttagagt ccaagagtct ttgtacctca taaaaatcct tctctgatat tataggttca
     1381 tgattgtttt caactactat ccaatcttct tcatctactg ctattcttct tctgtcctta
     1441 tatgagcgtg tagtttccct ttccttgggt taatgttcct atgtatattg gatttcttaa
     1501 tatcctattt atagtggtat gaccccatag gttataatca cttacattag gtaatgagaa
     1561 gttttcatat ttccttttca tatag
//
LOCUS       DATGSI010000384         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_307640, whole genome shotgun sequence.
ACCESSION   DATGSI010000384 DATGSI010000000
VERSION     DATGSI010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307640"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..944
                     /locus_tag="VK031_05950"
     CDS             78..944
                     /locus_tag="VK031_05950"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HLS53494.1"
                     /translation="MKIEIKNVSYIYNQGNPYEKKALDSVNLEINTGEFIGIIGHTGS
                     GKSTLVQHLNGLMVPTSGNVIVGGIDTKDNKASKIDIRKNVGLVFQYPEHQLFEETIY
                     KDIAFGPKNLGCTEEEIEERVKWACDLVGLDFENMKDESPFELSGGQKRRVAIAGVVA
                     MKPKFLVLDEPTAGLDPKGRDEILDQVKMLHEKEGITVILVSHSMEDVAKLVDRLIVM
                     DDSKVFMDGSPREVFSKKDELKSIGLGIPQVMDFMIRYKEMGHDISTDVLTVEEAYEV
                     LSKYLREGKDVR"
     gene            934..1731
                     /locus_tag="VK031_05955"
     CDS             934..1731
                     /locus_tag="VK031_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HLS53495.1"
                     /translation="MLDNMTIGQYFPGDSIIHKLDPRVKIAAVTLYIIAIFMITKFIV
                     YIPVALMIALVLYITKIPVKSVLKGLRPIIFIVLLTFIINIFMTPGEVVFKAGIFSIT
                     KEGIRTGSFMAFRLIFLVLGTSLLTLTTSPISLTNGLEDIMKPLSKIGFPSHQLAMMM
                     TIALRFIPTLFEETDKIRKAQMARGADFESGNIIARAKNMIPLLVPLFINAFRRADDL
                     AVAMEARCYRGGDDKTRLNELKYEKRDKVAYIVMILLFAIIIATRYI"
BASE COUNT          689 a          205 c          354 g          571 t
ORIGIN      
        1 cttgcatatt tattaaataa aaacggtttt aatgtttcta aagatgttct gagtatagat
       61 gatatggtga atgcaatatg aaaatagaaa taaaaaatgt tagttatata tacaatcaag
      121 gcaaccccta tgaaaaaaaa gctttagata gtgttaattt agagataaac acaggcgaat
      181 ttatagggat aattggccat acagggtctg gaaaatcaac tttagtacaa catttaaatg
      241 gtttaatggt accaactagc ggaaatgtaa tagtaggtgg catagatact aaggataata
      301 aggcttcaaa aatagatatt agaaaaaatg taggtctagt ttttcaatat ccagaacatc
      361 aattatttga agaaacgatt tataaagata tagcttttgg tcctaaaaat cttggatgta
      421 cagaagaaga gatagaagaa agagtaaaat gggcttgtga tttagtaggc ttagattttg
      481 aaaatatgaa ggacgaatcg ccatttgaac tatcaggtgg tcaaaaaaga agggtggcta
      541 tagctggtgt agtagctatg aaacctaaat ttttagtgtt agatgaacct acagcaggac
      601 ttgaccctaa aggtagagat gagatattag accaagtgaa aatgcttcat gaaaaagaag
      661 ggataactgt aatattagtt tctcatagta tggaggatgt agctaaatta gtggataggc
      721 ttatagttat ggatgatagc aaagttttta tggatggttc tcctagagaa gttttctcta
      781 aaaaagatga gctaaaatct ataggtcttg gaataccgca agttatggac tttatgatta
      841 gatataaaga gatgggacat gatatttcaa cagatgtttt aacggtagaa gaagcctatg
      901 aagtattaag taaatattta agggagggta aagatgttag ataatatgac aatcggtcaa
      961 tactttcctg gagatagtat tattcacaaa ctcgatcctc gagtaaaaat agcggcagta
     1021 acattatata taatagctat atttatgata actaaattta tagtttatat accagtagct
     1081 ttaatgatag ctttagtatt atatattaca aaaataccag taaagagtgt attaaaaggg
     1141 cttagaccta taatatttat agtcttactt acttttataa taaatatttt tatgacacct
     1201 ggggaagtgg tatttaaagc tggaatattt agcataacta aagaaggtat aaggacagga
     1261 agttttatgg cttttagact tatattttta gttttaggaa catctttatt gacgttaact
     1321 acaagtccga tatctctaac taatggactt gaagatataa tgaaacctct ttcaaaaata
     1381 ggttttccaa gtcatcaact tgctatgatg atgactatag cattgagatt tatacctaca
     1441 ttatttgaag aaactgataa aataagaaaa gctcaaatgg ctagaggtgc agattttgaa
     1501 tcgggaaata taatagccag agctaaaaat atgattccac ttttagttcc attatttata
     1561 aatgccttta gaagagccga tgatttagca gttgctatgg aagctagatg ttatcgaggc
     1621 ggcgatgata aaactagatt gaatgaacta aaatatgaaa agagggacaa agtagcttat
     1681 atagtaatga tacttttatt tgctattata atagctacta gatatattta atgagaaata
     1741 ttaagttaat tatacaatac gacggctcat attattcagg atggcaaaag caagatgatg
     1801 aaaggcttat aaccattca
//
LOCUS       DATGSI010000385         3695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_73150, whole genome shotgun sequence.
ACCESSION   DATGSI010000385 DATGSI010000000
VERSION     DATGSI010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73150"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2176
                     /locus_tag="VK031_05960"
     CDS             <1..2176
                     /locus_tag="VK031_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HLS53496.1"
                     /translation="VYFITSSVSKHIMDNMMDESRRLADSYMNRLSQNAMASKIIDDL
                     LEEKLLTANKVTLQSRDKVNNEFLEELAQILNVDEIYWYNQSGKIIYANRKEYLGWQA
                     SEGHPVYDFMEDEDDIYIGPIREDTESGIHFKYSYAKARDGQFIQIGIAAETIHQLTK
                     TFYHQRLIDKIKEQDDILDIFFLDNDGQVILAGENGYGLTYKPSKQVKDAIKRDEIYY
                     IRDKYRGSDAYKGFLPIYVDENKIGTLAIIYSLEASNDLIKKVSGLVILFLGLVFAVS
                     GGLLINIMRKNKRIEKLAYYDPITDSLNRNYFMAFFNYKLSQDTDKKRALMIINNTNL
                     RLVRSIYGYQASNELFRKKVKQLRKLDIPHNHLFRFSEGDFFLYVEGYKDKEDLEKLS
                     EQILNLFGEISKSVLSSRFITVKVGILKLDERYKDVDHLIKDAEILIGQLRKLEDKKY
                     CFFDETMQARAILDEKIIEELRKAEYKGFDKEFYLEYQPLLDLKTNKVVGLEALARWK
                     NKNLGFVSPKKFIEIAERQQLIVPLGEWILRTACSFLKEIENKGFDKIKMAVNISVIQ
                     LLQEDFSEKLMEIIEDTGVKTSNLELEITESNLMINYSIVNRVLDQLREEGISISLDD
                     FGTGYSSLYRLRKLNIHVIKIDKSFIDNIDRADQKDVFIESIITLAEQLDLKVVAEGV
                     ETQEQKKYLEKINCDIMQGYLFSKPVSNTEALRLLKERSKVN"
     gene            2263..2997
                     /locus_tag="VK031_05965"
     CDS             2263..2997
                     /locus_tag="VK031_05965"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLS53497.1"
                     /translation="MAPKIIFFTRKKHLYLISVLIIGIATINILPSEAIKNVISSSKI
                     KPLSKRIIVVDPGHGGIDGGTHYGEILEKDINLAIGLKLRDQLIKKGAKVNMTRERDA
                     SLEDYISNGSRHSRDLKARVGIIDASKADIFVSIHVNHIKNVNRMGPIVFYYEKSQKG
                     KSLAEAIQKQLNKLSEYKKEGIEINRIPMTGDYYILKNTKVPGVIIETGFISNQIDRS
                     LLQDEGHQEEIIELIIKGIIAYLNQQ"
     gene            3079..3240
                     /locus_tag="VK031_05970"
     CDS             3079..3240
                     /locus_tag="VK031_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53498.1"
                     /translation="MGEKIIKGWNLENSYIHLPKTFYKKEKPEIVPNPKLVILNEDLA
                     ATLGLNAKT"
     gene            3261..>3695
                     /locus_tag="VK031_05975"
     CDS             3261..>3695
                     /locus_tag="VK031_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HLS53499.1"
                     /translation="MLSGNRLPTNSIPIAQAYAGHQFGHFTMLGDGRAVLLGEQISPD
                     GKRLDIQLKGSGRTAYSRGGDGKAALGPMLREYIISEAMYNLDIPTTRSLAVVTTGEK
                     IARQGRPAGAVLTRVASSYIRVGTFQYAAAFGTEDELRMLAEY"
BASE COUNT         1376 a          490 c          777 g         1052 t
ORIGIN      
        1 agtttacttt ataacaagtt cagttagcaa acatataatg gacaatatga tggatgaatc
       61 cagaagactg gctgatagtt atatgaacag attgtcccag aatgcaatgg catccaagat
      121 tattgatgac ctgttggaag aaaaactgct aactgccaat aaggtaactt tacaatctag
      181 agataaggtc aataatgaat ttttggaaga actggcccag atcttaaatg tggatgaaat
      241 atattggtac aatcaatctg gcaaaataat atatgcaaac aggaaagaat atctgggctg
      301 gcaggccagt gagggtcatc cagtatatga ttttatggaa gatgaagatg atatttatat
      361 aggacccatt agagaggata ctgaatcagg aatccatttt aaatatagtt atgccaaagc
      421 aagggatgga cagtttattc aaataggtat tgcggctgaa accattcacc agctgacaaa
      481 aaccttctac caccagcgtt taatagataa aataaaagag caggatgata ttttagatat
      541 atttttttta gataatgatg gacaggtgat cttggctgga gaaaatggat atggattgac
      601 ttataagcca agcaaacaag ttaaagatgc aatcaaaagg gatgagattt actacataag
      661 agataaatat cggggaagtg atgcctacaa gggattttta cccatatatg tggatgaaaa
      721 taagattgga accctagcca taatatattc cttggaagct agtaatgatt tgattaaaaa
      781 agtttcaggt ttggttatcc tgtttcttgg cctagttttt gccgttagtg gtggcttatt
      841 aataaatatt atgagaaaaa acaagagaat agaaaagtta gcttattatg atcctataac
      901 tgattcctta aatcgtaact atttcatggc atttttcaat tataaattaa gccaagatac
      961 agataaaaag agagccctca tgattataaa caacacaaac ctaaggttgg taaggtccat
     1021 ttatggctat caagcatcaa atgaactttt tagaaaaaaa gtaaagcagc taaggaaatt
     1081 agacattccc cacaaccatc tatttagatt ttccgaagga gatttcttct tatatgtgga
     1141 aggatataaa gataaggaag atttagaaaa acttagtgaa cagatcttaa acctatttgg
     1201 agagatatct aaatctgtat taagtagtag atttattact gttaaggtag gtattcttaa
     1261 attggatgaa agatataagg atgtggatca tctaattaag gatgcagaaa tcctaattgg
     1321 ccaactcagg aagctggagg ataaaaaata ttgtttcttt gatgaaacca tgcaggcaag
     1381 ggctatttta gacgaaaaaa ttattgagga attaagaaag gctgaatata agggctttga
     1441 caaagaattt tacctagagt accagccctt gcttgactta aaaactaata aggttgtagg
     1501 tctagaagcc ttggctcggt ggaaaaacaa aaatttaggc tttgtatcac ctaaaaaatt
     1561 tattgaaatt gcagagaggc agcaattaat agtaccttta ggggagtgga ttttaaggac
     1621 agcctgtagt tttttaaaag aaattgaaaa caagggtttt gataagataa agatggctgt
     1681 taatatctct gtaattcaat tgttacagga ggatttttct gaaaaattaa tggaaataat
     1741 agaggatact ggagtaaaga caagtaatct ggagctggaa attacagaaa gcaatttaat
     1801 gattaactat agtatagtaa atagagtcct tgaccaatta agagaagagg gaattagcat
     1861 atccttagat gattttggga caggctattc atccctatac aggcttagga agttaaatat
     1921 acatgttatt aagatagaca aatcatttat cgataatata gatagggcag atcagaagga
     1981 tgtgtttatc gaaagcataa taaccctggc tgaacagtta gacctaaagg ttgttgctga
     2041 aggggttgaa acccaggaac agaaaaaata tttagaaaag atcaattgcg atattatgca
     2101 aggttattta tttagcaagc ctgtatcaaa cacagaagct ttgagattac taaaggagag
     2161 aagtaaagta aattaaatat ttatatatta attaatcata tggtgaatat actaattagt
     2221 atagtagtat tcaccatatc tttatttcta tggggtgatc aaatggcgcc taaaataatt
     2281 ttttttacaa gaaaaaaaca cctttatttg atatcagtcc ttataattgg aattgctacc
     2341 ataaatattc taccttcaga ggctataaaa aatgtaatta gtagtagcaa aattaagcct
     2401 ttatctaaaa gaattatagt agtggatcca ggacatgggg gaatagatgg aggaactcat
     2461 tatggagaaa tcttggaaaa ggatataaac ttagccatag gacttaaatt aagagaccaa
     2521 ctgattaaaa aaggtgcaaa ggtcaatatg accagggaaa gagatgcatc attggaggac
     2581 tatataagta atgggagtag gcatagccgg gatctaaagg caagggttgg aataatagat
     2641 gccagcaagg cagatatttt tgttagcata catgtgaacc atattaaaaa tgtaaacagg
     2701 atggggccta tagtttttta ctacgaaaaa agccagaagg gcaagtcctt agcagaagcc
     2761 attcaaaaac agcttaataa gctttccgag tataaaaaag aagggataga aataaataga
     2821 atccctatga caggagacta ttacatattg aaaaacacta aggtgcctgg ggtaattata
     2881 gaaactggct ttatatcaaa ccagatagat agaagtcttt tgcaggatga agggcatcag
     2941 gaggagatta tagagctgat tattaaggga ataattgctt acctaaatca gcaataaatc
     3001 aggctaatat ttccttggtt tatttgattg tttttacaaa taataagata aagtaaattt
     3061 gaaaggaata ctgataaaat gggagaaaaa ataattaagg gctggaattt agaaaatagc
     3121 tacatccacc tgccaaaaac tttttataaa aaagagaaac cagaaattgt acctaatcca
     3181 aaattagtta tattaaatga agatttagcg gcaactttag gtttaaatgc caagacttaa
     3241 aaagcaaaaa aggaatagaa attttatcag gtaataggtt gcctacaaat tctataccta
     3301 ttgcccaggc ctatgcaggc caccaatttg gccactttac aatgttagga gatggtaggg
     3361 ctgtactttt aggtgagcag attagtcctg atgggaaaag gttggatatt caattgaagg
     3421 gttccggtag aactgcctat tctagaggtg gcgatggcaa ggccgcccta ggacctatgc
     3481 taagggaata tattataagt gaagccatgt ataatttaga tatacctact actaggagtc
     3541 ttgctgtagt aactacggga gagaaaattg ctcggcaggg caggcctgct ggagcagtgc
     3601 tgacccgggt ggcttcaagt tatataaggg ttggaacttt ccaatatgct gcagcctttg
     3661 gtacagaaga tgaattaagg atgctggcag aatat
//
LOCUS       DATGSI010000386         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_216344, whole genome shotgun sequence.
ACCESSION   DATGSI010000386 DATGSI010000000
VERSION     DATGSI010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216344"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VK031_05980"
     CDS             complement(<1..275)
                     /locus_tag="VK031_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53500.1"
                     /translation="MEKRRIPKRLGTLLMITTLIITFSLATRANKVEFNLTQEEIDYV
                     KDSKVLKATSIIGGAPLHYIDSGGHVAGIAVGILENIAETTGLQFEY"
     gene            complement(530..1456)
                     /locus_tag="VK031_05985"
     CDS             complement(530..1456)
                     /locus_tag="VK031_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53501.1"
                     /translation="MKSNNGRLENILKGEGKKLTDLLNKKVEHERFGVGNVINCDDSY
                     IKIRFPSGDKKFVFPDAFKKYLTINDKSAAALVKEKLKEREKEREKEKIQLKKKREEK
                     ALRRAQLRMMEKQKSARRSVHSNAQSVFWCKPEELEDIFTDWQIFTGRIKSGVKKGQP
                     RILARMRQNSACLITEREADIEEKDRQILGVFMTKMDFNGRECEEGYIDAHPKYRIKL
                     SSEEADQMPFWKYYKNNRYPESIVWNSGRQRYFANKWMAQILRDIADLRKGTEEEELA
                     QEFLEYFCRINNIELEELPEPAGALVEIENQE"
     gene            complement(1784..>2645)
                     /locus_tag="VK031_05990"
     CDS             complement(1784..>2645)
                     /locus_tag="VK031_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLS53502.1"
                     /translation="RVSPEHKVRIVSALKANGDIAAMTGDGVNDALALKRSDIGVSMG
                     ITGTDVAKNTADLILTDDNFASIVSAVEEGRIIYDNIKKFVFFLLSCNIGEILLVFVS
                     ILLNLPVPLIPIQLLWLNLITDSFPALALGVEKGDPDIMRQEPRDPEEPILNKEMIAG
                     IVLQSIAIAVASLSAYWWGINRYGLDNILKARTVAFATLITAELLRAFSARSSKYTIF
                     EIGILSNKKLIQGILLSFSLMLIIIYIPFLNPIFNTFPLGLDDWKIILSFGFIPLLLG
                     EIVKLVRKRN"
BASE COUNT          786 a          540 c          333 g          986 t
ORIGIN      
        1 atatattcaa attgtaagcc tgttgtttct gcaatatttt caagtatacc tactgcaata
       61 cctgccacat ggccacctga atctatataa tgtaatggag cgcctccaat aatagaggtt
      121 gcctttaata ctttgctatc cttaacataa tctatttcct cttgggttaa attgaactca
      181 accttatttg ccctagtggc taaactaaat gtaataataa gagttgttat cataagtaat
      241 gtgcctaacc ttttaggtat tcttcttttt tccatcccac tttccccttt aattcttccc
      301 agttaccttt atctaatatg actatgtaac tggttaataa ttgaacctgt tacaattata
      361 tatcattaaa taaaatattt cattgttttt gttttgtata ttattacaat tacctgataa
      421 atttgtggaa tatgtttggc cgtaggctga agttagcccc ggctttaagt atgtctaaat
      481 aaaaaaggaa agcctatttt agctttcctt ttctcatctt tgaaatttat tattcttggt
      541 tttctatctc aactaaagca ccagcaggtt ctggaagttc ttccaactct atattattaa
      601 ttctacagaa gtattctaaa aactcctggg ccaattcttc ttcctctgta cccttacgta
      661 aatcggcaat atcccttaat atttgggcca tccacttatt agcaaaatat ctctgtcttc
      721 ctgaattcca aactatactc tcaggatatc tgttattctt gtaatatttc caaaagggca
      781 tctgatctgc ctcttcactt gatagcttaa tcctatattt gggatgggca tctatatatc
      841 cctcttcaca ttctctgccg ttaaaatcca tcttggtcat aaatacacct aaaatctgcc
      901 tatccttctc ttctatgtct gcttccctct ctgtaataag gcaagcactg ttttgtcgca
      961 ttctagctag tattctaggc tgtccctttt taactccact ttttatcctt ccggtaaaga
     1021 tttgccaatc agtaaaaata tcctctaatt cctcaggttt gcaccagaat acagactggg
     1081 cgttggaatg aacacttctt ctggctgatt tttgtttttc catcatccgt aattgagccc
     1141 tacgtaaggc cttttcttcc cttttcttct taagctggat tttttccttt tcccgttcct
     1201 tttccctttc cttcagcttt tccttcacca atgcagcagc actcttatca ttaatggtca
     1261 aatacttctt aaaggcatca ggaaatacaa actttttatc ccctgagggg aagcgtattt
     1321 taatataaga atcatcacaa ttaattacat ttcccactcc aaacctttca tgctccacct
     1381 ttttatttag tagatctgtc aatttttttc cctcaccttt caatatattt tcaagccgtc
     1441 cattgttaga tttcaagagg ttagtcattg tctcatactt acttttattt tatcacacta
     1501 agaggaaaat atcaaattat ctattgacat tgatatatat tctatgtata atattgtttt
     1561 tatgctttct agtaagtaat tttgtacagt tttctagagt aatttaagca ggagatttaa
     1621 attttatatt tttgctacag aatcaaagtc aaaataataa ggattcctaa ttgacccaaa
     1681 ttaagaatcc ttattattga taatatttcc ttccctctgc ttttgaaaca tctctacagt
     1741 cttttaatta ttcttatata taaagcttat ctaaaatcta taatcaattt cttttcctta
     1801 ctaactttac tatttctccc aataataagg gaataaaacc aaatgataat attatcttcc
     1861 aatcatctaa ccctagtgga aatgtattaa atattggatt taaaaatgga atatagatta
     1921 ttattaacat caatgaaaat gataataata ttccctgtat tagtttttta ttagacaata
     1981 taccaatttc aaatattgta tatttggatg atctagcaga aaaagctctt aataactcag
     2041 ctgtaattag tgttgcaaag gccacagtcc ttgccttaag tatattatct agtccatatc
     2101 tattaattcc ccaccaataa gctgataagg aagctactgc aatagcaata ctttgtaaca
     2161 caatcccagc tatcatctcc ttatttagta ttggttcttc agggtcccta ggttcttgcc
     2221 gcataatatc aggatccccc ttttcaacac caagtgctaa ggctgggaaa ctatctgtta
     2281 ttagattcaa ccaaagtaat tgtataggaa taagtggtac gggtaaattt agaagtatac
     2341 taacaaatac caatagtatt tctcctatat tgcaagataa taaaaagaat acaaactttt
     2401 ttatattatc atatataatt ctaccctctt ccactgctga aacaatactt gcaaaattat
     2461 catcagtaag aattaaatcc gctgtatttt ttgcaacatc cgttcctgta atacccattg
     2521 aaaccccaat atctgatctc tttagggcta gagcatcatt tacaccgtct cctgtcattg
     2581 cagcaatatc tccatttgcc tttaatgctg aaacaatcct taccttatgc tcaggtgaaa
     2641 ctcga
//
LOCUS       DATGSI010000387         1307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_258624, whole genome shotgun sequence.
ACCESSION   DATGSI010000387 DATGSI010000000
VERSION     DATGSI010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258624"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VK031_05995"
     CDS             complement(<1..881)
                     /locus_tag="VK031_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583158.1"
                     /note="phosphotriesterase homology protein; PhP; YhfV;
                     member of a family of proteins related to
                     phosphotriesterase (PTE); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HLS53503.1"
                     /translation="MIETIKGKIENLGKTYIHEHIKLDLSSHKSDPDTNYNDVDQLIL
                     EFKELKGKGISSIVEVTNRGMGRDILSLRKVADETGLNIIASTGFYKEPFLPEYFYRL
                     GNKELEKLLLKDIQKGIDGTDSKAHVLGEVGTSHNKITPMERRAFDIVTNVHLETGKP
                     IFTHTTLGTMALEQIEILKNKSVDLEKVLIGHMDLNFNKEYHLRVADTGCYLGFDTIG
                     KINYQPDEHRIELIKELIKRGHEDQIVLSLDLTRKSHLKNRGGIGYSYLIDIFIPMLI
                     DSGVSEKIIDKFLIDNP"
     gene            complement(1094..>1307)
                     /locus_tag="VK031_06000"
     CDS             complement(1094..>1307)
                     /locus_tag="VK031_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HLS53504.1"
                     /translation="KVLDIALLVGGMLAANAIAPTIGYIWVIGLYFLNQVSKKPLASM
                     AIGPIGAITMGLIVNVLHLLGLFPIS"
BASE COUNT          375 a          245 c          183 g          504 t
ORIGIN      
        1 tctggattat ctattaaaaa cttatctatg attttctcgc taacacctga atcaattaac
       61 atgggtataa aaatatcaat taaatatgaa tatcctatac cccctctatt ttttagatgg
      121 gatttccttg ttaaatcaag ggatagaaca atttgatctt catgtcctct tttaattaat
      181 tctttgatta attctatcct atgttcatca ggctgataat ttattttccc tatggtatca
      241 aatcctaagt aacaacctgt atctgctact cttaaatgat attctttgtt aaagtttaaa
      301 tccatgtgtc ctattaatac tttttctaga tcaacacttt tgtttttaag tatctctatt
      361 tgctccagtg ccatggtccc taaagttgta tgggtaaaga ttggtttccc ggtttctaaa
      421 tgcacatttg tcactatgtc aaaggcccgc ctttccatag gggtaatttt gttatgactt
      481 gtccctactt ctccaagtac atgagccttg gaatctgtgc catctattcc cttctgaata
      541 tcttttagta aaagtttttc taattcttta ttacctagcc tataaaaata ctcaggcaaa
      601 aaaggttcct tataaaagcc ggtgctggca attatgttta aacctgtttc atctgccact
      661 tttcttaagg aaaggatgtc cctacccatg cctctattgg taacctctac tatggaactt
      721 attccctttc cctttaattc tttgaactct aagatgagct gatccacatc attgtaattt
      781 gtatcaggat ccgatttgtg actggatagg tctagtttta tatgttcatg tatataggtt
      841 ttgcccaagt tttctatttt tccttttata gtttcaatca tatccattcc tctttccttt
      901 ttgctttgtt gaacaaatgc cttatttact ttagataaac ttgtcaatac ttttacttag
      961 ataaagaaga taaggtcttt tatactaaga aattatttta tgtataaagt attcacaaga
     1021 ttgtctaaat atattttata taaggttaag ggaggtatct cccctaacct ttagtatcaa
     1081 tctttttagg ctattatgaa attggaaata gtcctaaaag gtgtaataca tttacaatta
     1141 gtcccattgt tattgcacca attggcccaa tagccatgga tgccagaggc ttctttgaaa
     1201 cttggtttaa gaaatataga ccaattaccc atatataacc tattgttgga gctattgcat
     1261 tagctgctaa cattccacct acaagtaaag ctatatccaa caccttt
//
LOCUS       DATGSI010000388         3643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_216572, whole genome shotgun sequence.
ACCESSION   DATGSI010000388 DATGSI010000000
VERSION     DATGSI010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216572"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VK031_06005"
     CDS             complement(<1..206)
                     /locus_tag="VK031_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HLS53505.1"
                     /translation="MAYGRKMLFKFFTLFLCLLIIFGASVIVSILYPIKYKDYINKYA
                     QRYNIDPFLVAAIINVESKYNKDAL"
     gene            complement(220..840)
                     /gene="coaE"
                     /locus_tag="VK031_06010"
     CDS             complement(220..840)
                     /gene="coaE"
                     /locus_tag="VK031_06010"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967142.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HLS53506.1"
                     /translation="MIHNKCKIIGLTGGIASGKSTASQILKDQGFQVIDADKIAREIV
                     EINKPAYRKIIKAFGEDILKENKEINRKKLGQLVFSNPKLLKKLNKITHPFILREIKK
                     QIDKKCERNKLIFVDIPLLFEIEKKIKKHNIIFDEIWLIYVDEYTQIKRLMKRNNFSK
                     EEAKERIKSQLPMEIKKEKSTKIIDNRGHIEALRKNIKQLLKNLPQ"
     gene            complement(827..3496)
                     /gene="polA"
                     /locus_tag="VK031_06015"
     CDS             complement(827..3496)
                     /gene="polA"
                     /locus_tag="VK031_06015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HLS53507.1"
                     /translation="MEKKKLMIIDGSSLLHRAFYALPLLTTKDGIYTNGVYGFLTMLY
                     RIQDSHKPDYICVALDKKGPTFRHKEYEEYKSNRQSAPSELAQQFPIIREVLDTMNIT
                     YLELDDYEADDIAGTLAKEGEEEGLEVILVTGDKDYLQLASDQVQVLLTRRGITEMET
                     FDRAKIVEEYGIEPEQFIDLKGLMGDSSDNIPGVPGIGEKTGLKLVKEFGNIDKVYEN
                     LDKVRGKRLKENLIEYKNTAYLSRKLSEIIRNVPLDVELEDLRMKDPDWEKLVSLYEE
                     FEFKSLLNRIPAEYSNREDDIEIEYKSEVIEGDYQAIVDKIQDEKKFAFKFLTETGNY
                     IEDDILAIGIKAGDSPVYYIDLQEDLDKFVDQFKSAFEDEEIEKIGYQLKADIAILFR
                     LGIQINNYTFDAIIGQYLIDPSQSNYSINNLSEEYLNFYGIDEEELLGKGRNKKSFKE
                     LKEEDRVDYLSFIIGSLYKLEGKMKKLIKKDNMEELFYQVELPLIEVLASMEYTGFKI
                     DRKELERLGEEYNKEINSLTEEIHQMAGMEFNLNSPKQMGEVLFEKLGLPVIKRTKTG
                     YSTNMEVLEKLKPQHPVIEKILRYRQIVKLKSTYIDGYINLINEKTKRIHSRFNQTIT
                     TTGRISSTEPNLQNIPIRTEDGRKIRKAFIAQKPDYTLVDADYSQIELRVLAHISQDE
                     KMIAAFKNNEDIHTKTASEVFHVPMEEVDGRLRDNAKAVNFGIVYGISDYGLSRDLDI
                     PRKEAREYIDKYLENYKQVKEFMTRTVENGKEKGYVETLLHRRRYVPELKARNFNVRS
                     FGERIAMNTPIQGSAADIIKIAMVRVYKELKKRGLKSRLLLQVHDELIIETHKSEIDE
                     IQLMLKEIMEDAIRLDVPLKVDIQIGDSWYDTQ"
BASE COUNT          910 a          727 c          538 g         1468 t
ORIGIN      
        1 agggcatcct tattgtattt gctttccaca tttattatgg ctgccactaa aaaaggatct
       61 atattgtatc tttgggcata cttatttata taatctttat actttattgg ataaaggatt
      121 gaaactataa cacttgctcc aaatattatt agtagacata aaaatagggt aaaaaattta
      181 aatagcattt ttctaccata agccaaggat atccctccat tactggggca agtttttcaa
      241 caactgttta atattttttc ttagtgcttc tatatggccc ctgttgtcta ttatttttgt
      301 tgatttttct ttctttattt ccattggtag ttggcttttt attctttctt ttgcttcttc
      361 cttgctaaaa ttatttcttt tcattaatct ttttatctgg gtatattcat ctacatatat
      421 taaccatatt tcatcaaata taatattatg cttttttatc ttcttctcta tttcaaataa
      481 taggggaata tctacaaata ttaacttgtt tctttcacac tttttatcaa tttgtttttt
      541 tatttccctt agtataaagg gatgagttat tttatttagt tttttcaaaa gcttaggatt
      601 ggaaaagacc agctggccta gtttttttct gttgatttct ttgttctcct ttaatatgtc
      661 ctctccaaag gctttaataa tttttctgta ggccggcttg tttatctcaa ctatctccct
      721 ggctattttg tctgcatcta ttacctgaaa gccctggtcc ttaagtattt ggctggctgt
      781 tgacttaccg gaggctatgc ctccagtaag accaataatc ttacatttat tgtgtatcat
      841 accaactatc tcctatttga atatccactt ttagtggcac atctaatctt atggcatctt
      901 ccataatttc ctttaacata agctgtattt catctatttc acttttatgg gtttctatta
      961 taagttcgtc atggacctgt agtaagaggc gggattttaa tcctcgtttt ttcaattcct
     1021 tatatactct taccatggct atcttgataa tgtctgctgc actaccttga ataggtgtat
     1081 tcatggctat tctttccccg aaggatctaa cattaaagtt tcttgccttt aactctggta
     1141 cataccttct tctatggagc agggtttcca catagccctt ttccttgcca ttctcaactg
     1201 tcctggtcat aaattctttt acctgtttat aattttctaa atacttgtct atatattctc
     1261 tggcttcctt tctaggaata tccaaatccc ttgatagacc atagtctgat ataccataaa
     1321 ctatcccaaa gttaactgcc ttagcattat ctctcagtct cccatccacc tcttccatag
     1381 gtacatggaa gacttctgat gcagttttag tatgaatatc ttcattgttt ttgaaggctg
     1441 ctatcatctt ttcatcctgg gaaatatggg ccaggaccct taattctatc tgggagtagt
     1501 ctgcatctac cagggtatag tctggtttct gggcaataaa agcctttcta atctttctac
     1561 catcttcagt tctaataggt atattctgta gatttggttc tgtactggat attcttccag
     1621 ttgttgttat ggtttgatta aacctagagt ggattctctt agtcttttca ttaattaggt
     1681 ttatatagcc gtcaatatag gttgacttta gctttacgat ttgcctatac cttaggattt
     1741 tctcaataac tggatgttgt ggctttaatt tttctaacac ttccatattg gtggaatagc
     1801 cggtctttgt tctttttata actggcaagc ctaatttttc aaagaggact tctcccattt
     1861 gtttaggcga gttaaggtta aattccatgc cagccatttg gtggatctcc tcagttaggg
     1921 agttgatttc cttgttatat tcttctccca gcctttccag ttctttccta tctattttaa
     1981 aacctgtata ttccatgctg gctaaaacct ctattaaggg tagctccact tgatagaaaa
     2041 gctcttccat attatctttt ttaattaatt tcttcatctt gccttctaac ttatataggc
     2101 tacctattat aaaggatagg tagtctaccc tatcttcctc tttcagttcc ttaaagcttt
     2161 tcttatttct tcccttgcct aaaagctctt cttcatctat tccatagaag tttagatatt
     2221 cttcacttaa attatttatg gaatagttgc tttggctagg atctataagg tactgaccta
     2281 ttattgcatc aaaggtatag ttgttaattt gaattcccag tctaaatagg atggctatat
     2341 ctgcctttag ttggtaacct atcttttcaa tctcttcatc ttcaaatgct gatttaaatt
     2401 ggtcaacaaa tttatctaaa tcttcttgca ggtctatata atagactggg ctgtctcctg
     2461 ccttgattcc tatggccaat atatcatcct ctatatagtt gccagtttca gttaaaaatt
     2521 taaaggcaaa ctttttctca tcttggattt tatcaactat ggcctgataa tctccttcta
     2581 ttacttctga cttatactct atttcaatat catcttccct gtttgaatac tcagctggaa
     2641 ttctatttaa aaggctttta aactcaaatt cttcgtatag gctgaccaat ttctcccaat
     2701 caggatcttt cattcttagg tcctcaagtt ccacatctag gggtacattt ctaatgattt
     2761 cacttaattt tctactaaga taggcagtat ttttatattc tattaggttc tcctttaatc
     2821 tttttcctct aaccttgtct aagttttcat ataccttatc aatattacca aattccttga
     2881 ctaattttaa tcctgttttt tcgccaattc ctggcactcc tggaatatta tcggagctat
     2941 ctcccataag tcccttgaga tctataaatt gctctggctc tataccgtat tcttcaacta
     3001 tttttgccct atcaaaggtc tccatttccg taattcctct tctagttaat aaaacctgca
     3061 cctggtctga ggccagttgt aggtagtcct tgtctcctgt tactaaaatt acctccaggc
     3121 cctcttcctc cccttccttg gctagagttc cggctatgtc atctgcctca tagtcatcta
     3181 gctccaggta ggttatattc atagtatcta gaacttctct aattattgga aattgttgag
     3241 ccagctctga aggggctgat tgtctgttgc ttttgtattc ttcatattcc ttatgtctaa
     3301 aggtaggtcc ctttttgtcc aaggctacgc atatatagtc tggcttgtga ctgtcttgga
     3361 ttctataaag catggttaaa aagccatata ctccattggt gtaaattccg tccttggtag
     3421 ttaaaagggg tagggcataa aaggccctgt gtaaaaggga actaccatca attatcataa
     3481 gttttttctt ttccatttaa tcactcctag ttcctgtctc atactcttta gtatacctta
     3541 ttataaatgt tcagtaaaca ttgggaaaaa taaaatattg cttattttat gtttctgtgt
     3601 taaaattagt attattaatt catgccgaag tggtggaact ggc
//
LOCUS       DATGSI010000389         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_24892, whole genome shotgun sequence.
ACCESSION   DATGSI010000389 DATGSI010000000
VERSION     DATGSI010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24892"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VK031_06020"
     CDS             complement(<1..873)
                     /locus_tag="VK031_06020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLS53508.1"
                     /translation="MFFSDEINQNNPKEVEEIKKFLSNFNVIYDRPDKTYVIRDRGQI
                     IATGSADRNVLKYFFVSDQYKGQGAIGIIYNSLLEYLFRQGYNSYFVFTTPDNKIVFE
                     SLGLKEVHSTDRVSLFEGGFYNYGSWIMAVKKRLGPKKGKRGAIIMNCNPMTLGHKYL
                     IKKALGQVDELIIFVVEEDRSIFPFIDRFKIMEEELKEEKDIRLIKSGPYIISQGTFP
                     TYFIKRKDEMLKVYTELDGGIFANRIGKDLEIDVRFLGSEPTDQVTLAYNHMLEKILK
                     AKGIEVQIIPRKALD"
     gene            complement(1009..2487)
                     /gene="citF"
                     /locus_tag="VK031_06025"
     CDS             complement(1009..2487)
                     /gene="citF"
                     /locus_tag="VK031_06025"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HLS53509.1"
                     /translation="MSYFNNFDIKNVKVLNKSEYPLKVTAKEKIREDIRELIKELDIK
                     DNSSLSFHHHLRNGDKVLNLVMKEVQKAGIKGITLVATSLFPVHKEMIPLLEDGTIKN
                     IVTSYITGDLAKAISHGKLEGHILMQSHGGRARSILNEEVEIDVAFIASPSADKMGNI
                     SGVNGQNSCGTLGYAIADSIKAEKVVAVTDSLLDYPNYPADITEDYVDYVLVVDSIGL
                     KEGIVSGTTRVTRDPIGLNIARDTLKAMKATGLLKDGFSYQSGAGGVSLAVTAYLREY
                     MIENNIKGSFASGGITGYLTDMLEEGLFDALFDVQCFDLQAIESLKRNPNHIRMSASQ
                     YGNCEVSAVVDNLDIVILGATEIDVDFNVNVTTGSDGVLMGGSGGHQDTAAGSQVTII
                     VSKLFSSRIPFIKDRVDVITTPGTTVDILVTERGISVNPLRQDLIENFKKAGIETIDI
                     KELKKYAEDVMGVPRPIEKTDRIIGFSEYRDGSIIDYIYQVK"
     gene            complement(2471..3331)
                     /locus_tag="VK031_06030"
     CDS             complement(2471..3331)
                     /locus_tag="VK031_06030"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HLS53510.1"
                     /translation="MNYRTLLFIPGNNPGMLQNSDILGADGLIIDLEDAVALSEKDAA
                     RTLVREYINSFETKTDIFIRINPLDSPYFYDDLEGIKDLDIEGIVLPKAGIQPMEKLD
                     EYLEENQLDFKIIALVETALGLELALDIVQRSDRVIGIFLGAEDLTLDLGAKRTKESK
                     EIEYARSRIIAAAKAAGIQAIDTPFTDTDDMEGLKVDTLNAKDLGMTGKAVINPRHVE
                     DVNELFSPTQEEVDYAIRVVEGVKVANEKGLGAFSIEGKMIDKPIILRALNTLKLSGE
                     YKEEYDELLQ"
     gene            complement(3321..3608)
                     /gene="citD"
                     /locus_tag="VK031_06035"
     CDS             complement(3321..3608)
                     /gene="citD"
                     /locus_tag="VK031_06035"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HLS53511.1"
                     /translation="MGKVEIRKMAKAGTVESNDILIMMSQGEGDIDIELESIVDKQYG
                     EDIKKVIHDTLVEAGVDNVKVIAKDKGALDFTIRARVKTCIARGSGDIDEL"
     gene            complement(3596..>3838)
                     /locus_tag="VK031_06040"
     CDS             complement(3596..>3838)
                     /locus_tag="VK031_06040"
                     /inference="COORDINATES: protein motif:HMM:NF017494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HLS53512.1"
                     /translation="DQEVVDSIVKNDAIYFAAIGGAAALIAKSVKSAEIVAYEDLGAE
                     AIRKLYVEDFPVTVINDTHGNDLYKMGREEYLEWAK"
BASE COUNT          986 a          855 c          590 g         1407 t
ORIGIN      
        1 atctaaggcc tttctaggaa ttatttgcac ttctattccc ttagccttta aaatcttctc
       61 taacatatga ttataggcta aggttacctg gtctgtaggt tctgaaccta aaaatcttac
      121 atcgatttcc aaatccttac ctatccgatt ggcaaaaatt cctccgtcta attctgtata
      181 aacttttagc atttcatctt ttctcttaat aaaataggtg ggaaatgttc cttgagaaat
      241 tatataggga ccacttttaa ttagtcttat atctttctct tcctttagct cctcctccat
      301 tattttaaac ctatctatga aggggaaaat agatctatct tcttccacca caaagataat
      361 aagctcatct acctggccta gggccttttt tattaagtat ttatggccta aggtcatagg
      421 attacagttc attataatgg cccctctttt gccctttttg ggacctaacc tttttttgac
      481 tgccataatc caacttccat agttataaaa tcctccctca aaaagggaca ccctgtcagt
      541 agaatggact tccttcaaac ctaaggattc aaagactatc ttattatctg gtgttgtaaa
      601 tacaaaataa gagttatagc cctgcctaaa caaatattct aaaagtgaat tatatatgat
      661 tcctattgcc ccttgacctt tgtactggtc agagacgaaa aagtatttta atacatttct
      721 atcagcagag cctgtggcaa ttatttgccc cctatctctt ataacatatg ttttatcagg
      781 cctgtcatag attacattaa agttgcttag aaattttttt atctcttcca cttcttttgg
      841 attgttttgg tttatttcat cgctaaagaa catattatcc ctctttgtat tagaaatcat
      901 atctcaagta tattaggaca gaaggagatt gtccatataa gtattctata tgaaccctcc
      961 ccttgtccaa accaataccc caatctttta gattgttggt atccccctct attttacttg
     1021 gtagatatag tctattatgc taccatctct atattctgaa aatcctatta tcctgtcagt
     1081 tttttctata ggtcttggca ctcccataac atcttctgca tattttttta attctttaat
     1141 gtctatggtt tctatacctg cctttttaaa gttttcaatt aaatcctgcc ttagcggatt
     1201 tactgatatg cctctttctg taactagtat atctacagta gttcctggag tggttattac
     1261 atctacccta tcctttataa atggaatcct agatgaaaat agcttagata ctatgatggt
     1321 tacctggcta ccggctgctg tgtcctggtg gcctcctgac ccacccatta ggactccatc
     1381 tgatcctgta gttacattta cattaaagtc tacatctatt tctgtggctc ctaagataac
     1441 tatatctaga ttatctacta ctgctgatac ttcacagtta ccatattggg aggcactcat
     1501 cctaatatgg ttaggatttc tttttaaaga ttctattgcc tgcaggtcaa agcattggac
     1561 gtcaaagagg gcatcaaata agccttcttc caacatgtct gtcaagtatc ctgttatacc
     1621 tcctgaggca aaactaccct ttatattgtt ttcaatcata tattctctta aataagcagt
     1681 tacagcaaga gatactcccc ctgccccact ttgatagcta aagccatctt ttaaaaggcc
     1741 tgtagccttc atggccttta aggtatccct ggctatgttt aatccaattg gatcccttgt
     1801 aaccctggtt gtacctgaga ctataccttc ctttagtcct atggaatcta ctactaatac
     1861 atagtctaca tagtcttctg ttatgtctgc tggataattt ggataatcca atagtgagtc
     1921 tgtaacagcc actacttttt ctgccttaat actatctgct atggcataac ctaaagtgcc
     1981 gcaggaattt tgcccgttga ctcctgaaat gttgcccatt ttatctgcac ttggtgatgc
     2041 aataaaggcc acatctattt ctacctcttc atttaaaata cttctggccc tgccaccatg
     2101 ggactgcatc agtatatggc cttctagctt tccatggctg atggccttgg ccaagtctcc
     2161 cgttatatag gaggttacta tgtttttaat agtaccgtcc tctaaaagtg gtatcatttc
     2221 cttatgaact ggaaacaggg atgtggctac tagggtaatc ccctttatgc ctgctttttg
     2281 aacctctttc attaccaggt ttaatacctt gtcaccattt cttaagtgat gatgaaagga
     2341 aagggaggaa ttatctttta tatctaattc tttgattaat tctcttatat cttctctaat
     2401 tttttccttg gctgtgactt ttaaaggata ttccgactta ttcagtactt tcacattttt
     2461 tatatcaaaa ttattgaagt aactcatcgt attcctcctt atactcacca cttagcttta
     2521 gggtgtttaa ggcccttaaa attataggct tatctatcat tttaccctct atggagaagg
     2581 ctcctaaacc tttttcattg gccaccttaa ccccttctac taccctgatg gcatagtcaa
     2641 cttcctcctg ggtcggtgag aataattcat ttacatcttc cacatgcctt ggatttataa
     2701 ctgcctttcc tgtcatgcct aaatccttgg catttaaggt atctaccttc aagccttcca
     2761 tatcgtctgt gtctgtaaaa ggagtgtcta tggcctggat acctgctgcc ttggctgcag
     2821 ctattattct agacctggcg tattcaatct ctttagattc tttagttctt tttgctccta
     2881 aatctagggt taagtcttct gcccctaaaa atatgcctat taccctgtct gatctttgga
     2941 ctatgtctaa ggccagttcc aagcctaagg ctgtttctac taaggctatg attttaaagt
     3001 ctagttggtt ttcttccaag tattcatcta atttttccat aggttggata ccagcctttg
     3061 gaagaactat gccttctatg tccaaatcct tgattccttc tagatcatcg taaaagtagg
     3121 gactgtctag tggattgatt ctgatgaaaa tatctgtttt tgtttcaaag gaatttatat
     3181 attctctaac cagggttctg gcagcatcct tttctgatag ggctactgca tcctccaggt
     3241 ctataattaa accgtctgcc cctaatatgt cagagttttg gagcatccca ggattgtttc
     3301 ctgggatgaa aagtaatgtt ctataattca tctatatcac cacttcctct ggcaatacaa
     3361 gtctttactc tggcccggat ggtaaagtct aaagctccct tatccttggc tataaccttt
     3421 acattatcta ctcctgcttc tactaaggta tcatggatta ctttttttat gtcttctcca
     3481 tattgtttgt ccacaatact ttctagctct atatctatgt ctccttcccc ttgagacatc
     3541 ataattaaaa tatcattaga ttcaactgtg ccggccttgg ccatcttcct tatttctact
     3601 ttgcccattc taaatattcc tctctaccca ttttgtacaa gtcattgccg tgggtatcgt
     3661 ttataactgt tactgggaag tcttccacat ataatttcct gatggcctcg gcacctaaat
     3721 cctcataggc tacaatttct gcagatttta ctgatttagc tattagtgca gctgctccac
     3781 ctatggctgc aaagtatatg gcatcatttt ttactatgct atctacaact tcctgatc
//
LOCUS       DATGSI010000390         1348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_450571, whole genome shotgun sequence.
ACCESSION   DATGSI010000390 DATGSI010000000
VERSION     DATGSI010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450571"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VK031_06045"
     CDS             complement(<1..398)
                     /locus_tag="VK031_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HLS53513.1"
                     /translation="MNVLFVCTGNTCRSPMAEGILKDMAIKENLDIQVKSAGTFTIKG
                     DQAATNAIEVMKDLGIDISNHSSRLVTEDLIKESDLVLTMANSHKDLLLNHYPEFKEK
                     IYTLNQYAYELEKDIADPFGGNKETYKRAR"
     gene            complement(411..>1348)
                     /locus_tag="VK031_06050"
     CDS             complement(411..>1348)
                     /locus_tag="VK031_06050"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HLS53514.1"
                     /translation="TVYGLGADGLNKRAVEKIFKAKTRPSDNPLILHISSIDELFPLV
                     QEVPDLAYRLIEKFWPGPLTLILKKSKQVPDIITAGLDTVAVRMPDHPIASSLIATAK
                     RPIAAPSANISGRPSPTRAEHVVEDMMGKIDMIIDGGETGVGLESTVLDLSSSTPTIL
                     RPGGVTYEELKKVLPNLGQDESIIEPDETPLSPGQKYRHYAPKAEMHLYIGDLEKIIE
                     TINKDSLQLIGQGKKVGIMATEESKAGYKKGIVKVVGSRKKMETIANNLFKTIREFDK
                     EDVDIILAEGVGLDNIGTAIMNRMIKASGGKLINI"
BASE COUNT          334 a          303 c          218 g          493 t
ORIGIN      
        1 tccctggccc ttttataagt ctccttgttt ccaccaaagg gatctgctat atctttttcc
       61 aattcatagg catactgatt taaggtatat attttctcct taaattctgg gtaatgatta
      121 aggagtaaat ccttgtggct atttgccatg gttaagacta gatctgattc tttaattaaa
      181 tcttctgtta ccagcctaga gctatgattg gatatatcaa tgcctaagtc tttcattact
      241 tcaatagcat tggttgctgc ctgatctccc ttaattgtaa aggtacctgc agactttacc
      301 tgtatgtcta aattttcctt aatagccata tcttttaata ttccttcagc cattggactc
      361 ctacatgtat tacctgtaca gacaaatagt acattcaatg ttttcactcc ttaaatgttg
      421 ataagttttc ctccagatgc cttaatcatt ctgttcatta ttgctgttcc tatgttgtct
      481 aagcctacac cttctgccaa tattatatct acatcttcct tatcaaattc cctaatagtt
      541 ttaaacagat tattggctat agtctccatt ttctttctgg agcctactac tttcacaatt
      601 cccttcttat aaccggcctt gctttcttcc gttgccatta ttcctacctt tttcccctgg
      661 cctataagct gtaagctatc cttatttatt gtttcaatta ttttctccaa atctcctata
      721 tataaatgca tctctgcctt aggagcatag tggcgatact tttgtcctgg agataagggt
      781 gtctcatcag gttcaattat ggactcatcc tggcccagat tgggaaggac ctttttcaat
      841 tcctcatagg ttacgccacc tggccttaga attgtaggag tagagctgga taggtctaaa
      901 actgtagact ccagacccac tcctgtttca ccaccatcaa taatcatatc tatttttccc
      961 atcatatctt ccacaacatg ttcagcccta gttggactag gccttccaga tatattggca
     1021 gatggggcgg ctattggtct tttagctgtg gctattaggc tacttgcaat aggatgatca
     1081 ggcatcctca cagccacagt atctaggcca gcagttatta tatctggaac ctgcttactt
     1141 tttttcagaa tcagggtaag aggtcctggc caaaactttt ctattagtct ataggctaaa
     1201 tctggtactt cctgtactag aggaaacaat tcatctattg agctaatgtg aaggattaaa
     1261 ggattatcgc ttggtcttgt cttggcctta aatatttttt ctacagccct tttatttagt
     1321 ccgtctgcac ctagcccata tacagttt
//
LOCUS       DATGSI010000391         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_217040, whole genome shotgun sequence.
ACCESSION   DATGSI010000391 DATGSI010000000
VERSION     DATGSI010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217040"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VK031_06055"
     CDS             complement(<1..744)
                     /locus_tag="VK031_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HLS53515.1"
                     /translation="MKTLKLIKKVAPEINEILERRYLILRGIQQNQPIGRRTLATELG
                     FKERTVRDEVAILKNQELLDVDFTGTYITEKGHKLLDDLHEIYNHLKGIPQLEEELRK
                     LLKIKKVIIVPGNSSENRMVLKDMGKITFKELKNNISSKDIIGITGGNTMASVVEEAV
                     YDKKERDILVIPARGGLGRNLNTQSNSIAAKLAEGLGGSYRLLYIPDALEEEALEYIL
                     KNEEISHSLNLINNMNTLVFGIGRADIMAK"
     gene            complement(937..2004)
                     /locus_tag="VK031_06060"
     CDS             complement(937..2004)
                     /locus_tag="VK031_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HLS53516.1"
                     /translation="MKGFAMKQIGKTGWVEKEKPECGPLDALCRPIAVAPCTSDIHTV
                     YEGAVGERTDMILGHEAVGEIIEVGELVKDFKPGDRVIVPAITPDWGAIESQRGFPMH
                     SGGTLGGWKFSNFKDGVFSEVFHVNEADANLAILPEELDPAEATMICDMIPTGFHGVE
                     LAEVTYGEDVLVIGIGPVGLMAVAGAALRGAGRILAVGTRPNCVALAKEYGATDIISY
                     KNGPIDQQVAELTKGKLVDKVIIAGGTVDTFAEAIKSVKPGGIISNVNYLGSGDFIKI
                     PREAWGVGMGHIRIVGGLMPAGRMRTERLANMLVNGRLDVSGMLTHKFEGFDSIPAAL
                     ELMREKPAELIKPVVTITYND"
BASE COUNT          555 a          510 c          292 g          734 t
ORIGIN      
        1 ttttgccata atatctgccc tacctatacc aaagactaag gtattcatat tgttgattag
       61 atttagggaa tggcttattt cctcattttt taatatatat tctaaagcct cttcctctaa
      121 agcatctgga atgtatagta acctatatga accacccagc ccttctgcca atttggcagc
      181 tattgaatta gactgggtgt ttaagtttct tcctaagcca cctctagccg gtataactaa
      241 aatgtctctt tcctttttat cataaactgc ttcctctact accgaagcca tggtgttgcc
      301 accagtgata cctataatat ccttagaact tatattattt tttagctcct taaaggtaat
      361 tttacccata tcttttaaaa ccatgcgatt ttcactggaa tttcccggca caataattac
      421 ctttttaatt tttaacagtt tccttaactc ctcttccagc tgaggtattc cctttagatg
      481 attatatatt tcatgtaggt catctaaaag tttatggcct ttttcagtta tataggttcc
      541 tgtaaaatct acatctaaca attcctggtt ttttagaatt gccacttcat ctctaactgt
      601 cctttcttta aaccctaact ctgtagccag agtccttcta ccaataggct ggttttgttg
      661 gatacccctt agtatcaaat accgcctctc aagaatctca tttatctctg gagctacttt
      721 ctttattaac tttaatgttt tcatttaatc actccgttag ttatatgtgg tggccttttt
      781 tcgtcccagt tatgtagatt ttgtcccagt ccctgtaaat aaaaagtgga aacaaatccc
      841 actacctata gtatacctta ttaccttatc ttattcaata gattttgata agaaatctat
      901 ctattccaga tgtcttaata ataaggatta gttatattaa tcattgtaag taatggtaac
      961 tactggttta atcaactctg ctggtttttc tctcattaat tccagtgcag ctggtatgct
     1021 atcaaaaccc tcaaatttat gtgttaacat accagaaacg tctaatctgc catttaccag
     1081 catattggcc aaacgttctg tacgcatgcg gcctgcaggc attaatccac ctacaattct
     1141 aatatgaccc attccaactc cccatgcttc cctaggaatc ttgataaaat cgccactacc
     1201 taggtagttt acgttgctaa taattccacc tggtttaact gatttaattg cttcagcaaa
     1261 tgtatctaca gttccaccag ctataattac cttatccacc agcttcccct tggtaagttc
     1321 tgccacctgt tgatctatag gtccattttt ataactgata atatctgtcg ccccatattc
     1381 cttggctaag gctacacaat ttggtcttgt tcccacggcc aatatccttc cagctcccct
     1441 taaggctgca ccggccacgg ccattaaccc tactggtcct atgcctatta ctaatacatc
     1501 ttccccatag gttacctctg caagctctac accatgaaaa cctgtaggta tcatgtcaca
     1561 aatcattgta gcttctgccg gatccaactc ctctggcaat atggctaggt ttgcatccgc
     1621 ctcatttaca tggaatactt cgctgaaaac tccatcctta aagtttgaaa atttccagcc
     1681 tcccagagtc ccacctgaat gcatagggaa tccacgttgt gattcaatag caccccagtc
     1741 tggtgtaatg gctggtacaa ttaccctatc tcctggctta aagtctttga ctaactctcc
     1801 tacctctata atttcaccaa cagcctcatg cccaaggatc atatctgtcc tctcgcctac
     1861 agcaccttca taaacagtat gtatatctga tgtacatggt gccactgcaa ttggacgaca
     1921 caaagcatca agtggtccac attctggttt ctccttttct acccaacctg tttttcctat
     1981 ctgcttcatt gcaaatcctt tcatttaatt acctcctaaa tttttttatc tacttgatgt
     2041 aggttataca tcaagtagat tgtcattatt gtaacaatcc ttttttaatc a
//
LOCUS       DATGSI010000392         1840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_73947, whole genome shotgun sequence.
ACCESSION   DATGSI010000392 DATGSI010000000
VERSION     DATGSI010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73947"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VK031_06065"
     CDS             <1..1161
                     /locus_tag="VK031_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLS53517.1"
                     /translation="PIEGEFEADSKNEVINMLREKNYLPVNIEADSGAEAELNIFNPT
                     VKKKELAIFCRQFYTMIDAGIGIVRCLDILVMQTENKTLKKGLQAVYEDVQKGFTISS
                     AMKKHEKIFPNILTNMVEAGEVSGNLDEIMARMADHFEKENRLENKIKSAMIYPIVLI
                     VVSIAVVIFMLVNVLPTFVSMFEGSGVELPWPTEFVLAISSSLQTYWYLFLAAIGLIV
                     AIFSYFKTTQEGRRLLDGIKLKIPYFKIINIKIITSRFTRTLSTLIGAGIPLIASMEV
                     VSRVVNNAVVEERIIYGIENIRKGAPLSRAVKDVEIFPPMVDSMINVGEESGALDEIL
                     QKTADFYDEEVEESLQRLTTLIEPLLIVIMALIIGFIVIAMALPMFDMVETI"
     gene            1226..1732
                     /locus_tag="VK031_06070"
     CDS             1226..1732
                     /locus_tag="VK031_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLS53518.1"
                     /translation="MLKFFTKKKKRKGFTLIELIVVIAILGILAAIAIPRLTGSRDRA
                     RRNAVISNLRTIESAIAVAEAEGALDKAVDGNTDGDTVKDILISKNQDGENQDGENQD
                     GKNQDGQSGNFLAAWPKGDNGVSYMIVEHEGIWRAAVELENDNPFDGLGGGTYILGEG
                     DHGLDEVE"
BASE COUNT          631 a          246 c          415 g          548 t
ORIGIN      
        1 ccaatagaag gtgagtttga ggctgatagc aaaaatgaag ttattaatat gcttagggag
       61 aaaaactatt tgccagtcaa tatagaggca gattctgggg ccgaagcaga gttaaatatc
      121 tttaatccaa ctgtaaagaa aaaggagttg gccatatttt gcaggcaatt ttacactatg
      181 atagatgcag gtatagggat tgtcagatgt ttagatatat tagtgatgca aacagagaat
      241 aaaactctaa aaaaaggctt gcaggcagtt tatgaggatg tacaaaaagg atttactatt
      301 tcttcagcca tgaaaaaaca tgaaaaaata tttccaaata ttttaaccaa tatggtggag
      361 gctggagagg tcagtggtaa cctagatgaa attatggcca ggatggctga tcattttgaa
      421 aaggaaaaca ggctggaaaa taaaataaaa tctgccatga tatatcctat agtacttatt
      481 gtagtgtcta ttgcagtggt tatttttatg ctagttaatg ttttacctac ttttgtgagt
      541 atgtttgagg gaagtggggt agaactacct tggcccactg aatttgtatt ggccatcagc
      601 agtagccttc agacttactg gtatctgttt ctggcagcta taggattaat agttgctata
      661 ttttcttatt ttaaaactac ccaagagggt aggaggcttt tggatggtat taagctgaag
      721 ataccttatt ttaagattat taatataaag attataactt cccgctttac taggacctta
      781 tccaccttaa taggagcagg tatcccctta attgcctcaa tggaagtggt tagtcgggta
      841 gttaataatg ctgtagtgga ggaaaggata atttatggaa ttgaaaatat tagaaagggt
      901 gcgcccttgt ctagggcagt aaaagatgtg gagatttttc cacctatggt agactctatg
      961 ataaatgtgg gagaagaatc aggagcctta gatgaaatcc tacaaaaaac tgcagacttt
     1021 tatgatgaag aggttgaaga aagcctgcaa aggcttacta cccttataga gcccttgttg
     1081 atagttatta tggctctaat aattggtttt atagtaatag ctatggctct acctatgttt
     1141 gatatggtgg agaccatcta agttattaag ctaggcttta actatttaat ataatttaaa
     1201 gcaataaata aagggaggaa aatatatgtt aaaatttttt actaaaaaaa agaaacgtaa
     1261 aggtttcaca ttaattgaat taatagtagt aatagcaatc ttaggtattt tagcagcaat
     1321 agccatacca agacttactg gatccaggga tagagccaga agaaatgctg tgataagcaa
     1381 cttaaggaca attgaatccg ctatagctgt ggcggaagct gaaggagcct tggataaagc
     1441 tgtcgatggt aatactgatg gcgatactgt caaggatatc ttgataagta aaaatcaaga
     1501 tggtgaaaat caagatggtg aaaatcaaga tggtaaaaat caagatgggc aatcaggtaa
     1561 cttcttagct gcttggccca agggagataa tggggttagc tacatgatag tagaacatga
     1621 aggtatttgg agggcagcag tagaacttga aaatgataat cccttcgatg gtttgggtgg
     1681 aggcacttat atacttgggg aaggagacca cggattagat gaggttgaat aataatcaac
     1741 acaatcatca ttattatata gacagggaag tttaccttcc ctgtccatat aaattattta
     1801 ggttaattta agtacttgat acaggtgaaa atatgacaat
//
LOCUS       DATGSI010000393         1063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_499501, whole genome shotgun sequence.
ACCESSION   DATGSI010000393 DATGSI010000000
VERSION     DATGSI010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499501"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..>1063
                     /locus_tag="VK031_06075"
     CDS             91..>1063
                     /locus_tag="VK031_06075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLS53519.1"
                     /translation="MKRIVIIGAGPGGYEAAVRAAQLGADVKLIEKNELGGTCVNVGC
                     IPTKALWEVARIRDDITRGADMGIHVKDLSLDPVGIYNRKNYVVNRMKGGIKFLMKSY
                     PNIEVIKGIGKLKNKNTVTVTTEDEETRDVEADAIIIATGSEVTMPEIEGINLDGVMT
                     SNDILELQEIPDSMVVIGSGVIGMEFTSIYSKFGTKIELIGRVMAKQADGEISRKLES
                     LVDVLNVKFRKGYRAEKIEEKGDKLYVTIYNKEKDDRIIVEGEKVLVASGRKAYMDKL
                     NADEIGIKSDKTGIFVDKDFKTNIDGIYAIGDVVSGNLQLAHLASAQG"
BASE COUNT          435 a           95 c          234 g          299 t
ORIGIN      
        1 tcaggtatat gaaattgaag aagatagatt tatggataaa gaagagtacg atgaatttat
       61 aaaacattgg taatttaaag gagatagatt atgaagagga ttgttataat aggtgcaggt
      121 ccaggaggat atgaagctgc agtaagggca gcacaattag gagcagatgt taaacttatt
      181 gaaaaaaatg aattaggtgg aacatgtgta aatgtaggat gtatacctac taaagctctt
      241 tgggaagtag cacgaattag agatgatata acaagaggag ctgacatggg tattcatgtc
      301 aaagatttaa gtctagatcc tgttggaatt tataatagaa aaaactatgt agtaaatagg
      361 atgaagggcg gaattaaatt tttaatgaaa tcctatccta atatagaagt tataaagggc
      421 ataggaaaat taaaaaataa aaatactgtt acagtaacta ctgaagatga agaaactaga
      481 gatgtagagg cagatgcaat aataatcgct acaggttcag aagttactat gccagaaata
      541 gaaggcataa atttagatgg tgtaatgaca agtaatgata ttcttgaatt acaagaaata
      601 ccggatagta tggtagttat tggttcagga gttatcggta tggaatttac tagtatttac
      661 tcaaaatttg gcacaaaaat tgaactaata ggtagggtta tggctaaaca agctgatgga
      721 gaaatttcta gaaaattaga atcattagtt gatgtgttaa atgtaaaatt tagaaaaggc
      781 tatagagcag aaaagataga agaaaaaggt gacaagttat atgttactat ttacaataaa
      841 gaaaaagacg atagaattat tgtagaagga gaaaaagttt tagtagcatc tggaagaaaa
      901 gcttatatgg ataagttaaa tgccgatgaa ataggtataa aatcagataa aacaggtata
      961 tttgtagata aagattttaa aactaatata gatggaattt atgctatagg cgatgtagtt
     1021 tcaggaaacc ttcaattagc tcatttagct tcagcacaag gta
//
LOCUS       DATGSI010000394         1671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_450916, whole genome shotgun sequence.
ACCESSION   DATGSI010000394 DATGSI010000000
VERSION     DATGSI010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450916"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VK031_06080"
     CDS             <1..1027
                     /locus_tag="VK031_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HLS53520.1"
                     /translation="HVNFLEEDELEDVEKNVGNMAITPLMYRLNPNRLGIPDLETSED
                     LPDSLDLEGIDIKLQDMVAKTGNMEKLDLDETPTNTGKQAKREFASQIDLSYSEKIVD
                     LRAMKDEKNITPLLFPELEDLEKLEENSAEIKEEELLRLMVDRIKDREIDTEIKEIDI
                     QENQINHNLNRLDGLIRQELIEEPKSQLDSHNMETIKASIIESMKVTKMEGSSLMEVQ
                     LHPKELGKVHIELKLEEGRISGRILVDNSSVKDLFVNSLGDLTSSLKKHDINMDNLDI
                     DLNKNYNSFNQDPNTNHNPGSNKNFQGQQMNYFKSANLFNLRSQVGKGVGGQTAEFKG
                     AGLNILV"
     gene            1038..1454
                     /locus_tag="VK031_06085"
     CDS             1038..1454
                     /locus_tag="VK031_06085"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HLS53521.1"
                     /translation="MRINPNYYNQLERTSEKGHNQLDINDFLQIMVAEIQSMNPLSSE
                     GGSKTDYIAQMVQFTTLEQMSDMAEGIGLLNLMGQQQYSFSLIGKEVTLEQGDQLITG
                     VVDKVKFQYGSILLEIDGKDYYLGDVVEVANKEVEK"
     gene            1451..>1671
                     /locus_tag="VK031_06090"
     CDS             1451..>1671
                     /locus_tag="VK031_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53522.1"
                     /translation="MTYKINRVQGQSFPGRGEKPSKTKLNKSLSFQNYLYKEIKKKDI
                     KISAHARERLKERQLDFQAKDMDRIKEAI"
BASE COUNT          666 a          249 c          359 g          397 t
ORIGIN      
        1 acatgtaaac ttcctagaag aagatgagct ggaagatgtg gaaaaaaatg taggaaacat
       61 ggccataacc cccttaatgt acaggctgaa tccaaatagg ttaggtattc cagaccttga
      121 gactagtgag gacttacctg acagtttaga tctggaaggt atagacataa agctccaaga
      181 tatggtagct aaaacaggaa atatggaaaa gttagacctg gatgagaccc ctacaaatac
      241 aggcaagcag gctaaaaggg aatttgccag ccaaatagac ttaagctaca gtgaaaaaat
      301 agttgattta agggccatga aagatgaaaa gaatattaca cccttacttt ttcctgagct
      361 agaagactta gaaaaactgg aagaaaattc agctgaaatc aaggaagaag agcttttaag
      421 acttatggtt gacagaatca aagataggga aatagataca gaaataaaag aaatagatat
      481 ccaagaaaac cagataaatc ataacctaaa taggctagat ggtttaataa ggcaagagct
      541 tattgaagaa ccaaaaagtc aacttgacag tcacaatatg gagaccataa aagcatctat
      601 aattgagtca atgaaagtta caaaaatgga aggcagtagc ctaatggagg ttcagttaca
      661 tcctaaggag ctaggcaagg tccatataga gttaaagctg gaagaaggca ggatcagtgg
      721 ccgaatcttg gtggataatt caagtgttaa ggatctattt gtaaatagtt taggtgattt
      781 aactagcagc ttaaaaaagc acgatataaa catggacaat ctagatatag acttaaataa
      841 aaactacaat agtttcaacc aagatcctaa taccaatcat aatcctggtt ctaataaaaa
      901 cttccaaggc cagcagatga attatttcaa gtcagctaat ttatttaact taagaagcca
      961 agttggaaaa ggggtaggag gccaaacagc tgaatttaaa ggggctgggc tcaatatttt
     1021 agtgtaagga ggataaaatg aggataaacc ctaattacta caatcagtta gaaagaactt
     1081 cagaaaaggg ccataatcaa ctggacatta atgacttttt acagattatg gtggcagaaa
     1141 tccaaagtat gaatccttta agttcagaag gtggcagcaa aactgattat atagctcaaa
     1201 tggttcagtt taccaccttg gaacaaatgt cagacatggc tgaaggcatt ggccttttaa
     1261 acctaatggg gcaacaacag tattccttta gcctaatagg aaaagaagtt accttagaac
     1321 agggagatca gctgattact ggtgtggtag acaaggtgaa attccaatat ggtagtattc
     1381 tcctagaaat agatggtaaa gattattacc taggagatgt agtagaagta gctaacaaag
     1441 aggtagaaaa atgacttata aaataaatag ggttcaggga caaagctttc ccggaagggg
     1501 agaaaagccg agtaagacta agcttaataa aagccttagc tttcaaaatt atttatataa
     1561 agaaataaag aaaaaggata taaaaatatc tgcccatgcc agagaaaggc ttaaggaaag
     1621 gcaactagac tttcaagcca aagatatgga ccgaattaag gaggccataa g
//
LOCUS       DATGSI010000395         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_451125, whole genome shotgun sequence.
ACCESSION   DATGSI010000395 DATGSI010000000
VERSION     DATGSI010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451125"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1062)
                     /gene="pdxA"
                     /locus_tag="VK031_06095"
     CDS             complement(25..1062)
                     /gene="pdxA"
                     /locus_tag="VK031_06095"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HLS53523.1"
                     /translation="MKKSIIGITMGDPASIGPEISAKVLSKEEIYKQCNPIIIGDSRT
                     MERALQLIDRKDIKVNPVEKVSDAQFKYGIIDVYDMQNIDIDELEPGKVSKEAGEAAF
                     RYVEKVIDLALSGEVDATVTNAFNKEAINLAGHNYSGHTEIYAEKTNTEKYTMLLAHD
                     NLRVVHVSTHVSLREACDLVKKERVLDVIRIANDVCIDLGIENPKIGVAGLNPHSSEN
                     GMFGNEEELEIIPAISEAISQGINAEGPVPPDTLFSKANGGMYDIVVAMYHDQGHIPL
                     KVVGFVYNEQEQRWEDVSGVNVTLGLPIIRASVDHGTAFGKAWKGTASELSLHNAIEF
                     ALKLAENKRRK"
     gene            complement(1086..1967)
                     /gene="dapA"
                     /locus_tag="VK031_06100"
     CDS             complement(1086..1967)
                     /gene="dapA"
                     /locus_tag="VK031_06100"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HLS53524.1"
                     /translation="MNIHGIITPNLTPMNSDETINYTGLRKEVDRLIEGGIHGIFPVG
                     TNGEAYALSFEEKVNIIRETVDQVAGRVPIYAGTGCVTTEETIRLSKEAEKMGVEALS
                     VITPYFAKIGQEEIYNHFKLVAESVNIPIILYNIPARTGNHIEAETAAKLAKLPNVRG
                     IKDSSGNFLNILAYIKIKEIREDFVVLAGNDALILSTLQAGGDGAVAGCSNVYPSLMA
                     DIYNKYKEGDQEGALENQNKIIEFRKLFRYGNPNTIVKKATDLMGHNVGECRSPFNHI
                     SIEGQEAIRSFVEQNKC"
     gene            complement(1979..>2584)
                     /locus_tag="VK031_06105"
     CDS             complement(1979..>2584)
                     /locus_tag="VK031_06105"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HLS53525.1"
                     /translation="IEKLPKKIIAYTGVDALCHAIECYTSNKANDISNLFSLEALKLI
                     YENLEQAYIKEDMKAKEKMLLAAYYAGVAIAAAGTTAVHALSYPLGGKYRMAHGLSNA
                     MLLLPVMKENKDVIEDRLEKVYDYIWGRDSSENKAEAVLQWMEELLRILEIPTSLKEF
                     NIDEKDLDYLAEAAFGVKRLLNNNMKEYDVETIKQIYLEIM"
BASE COUNT          665 a          602 c          358 g          959 t
ORIGIN      
        1 atgataaagt tctttttctc atttttattt cctcctttta ttttctgcta attttaatgc
       61 aaattcaata gcattatgca agcttagttc acttgctgtt cccttccatg cctttccaaa
      121 ggcagttcca tggtctacag atgccctgat tatcggcagt cctaaagtta cattaactcc
      181 agaaacatct tcccatcttt gttcctgttc attatataca aaacctacta cctttaaagg
      241 tatatggccc tggtcatggt acattgctac tacaatgtca tacattccac cattggcctt
      301 agaaaacagt gtatccggtg gaactgggcc ttctgcattt atcccctggc ttatggcttc
      361 actaatagct gggattattt ctaattcctc ttcatttcca aacattccat tttctgagct
      421 gtggggattt aatcctgcca ctcctatttt ggggttttca atgcctaaat ctatgcaaac
      481 atcattggct attcttatta catctaagac ccgctctttc ttaaccagat cgcaggcttc
      541 ccttaaagac acatgagtgg acacatgtac tactcttaaa ttatcatgag ctaataacat
      601 agtgtatttc tctgtatttg tcttttctgc atatatttct gtatgtcctg aatagttgtg
      661 ccccgccagg tttatagcct ctttattaaa tgcattggta acagttgcat caacctcacc
      721 tgataaggcc aggtctataa ccttctccac atatctaaat gctgcttctc ctgcctcttt
      781 ggataccttt cctggttcaa gctcatctat gtcaatattt tgcatatcat acacatctat
      841 aatcccgtat ttaaattgag catcagacac cttttctact ggatttactt taatatcctt
      901 cctatctata agctgcaagg ccctttccat agtcctagaa tctcctataa taataggatt
      961 acattgtttg tatatttcct cctttgataa aacttttgca ctaatctcag gtccaatgct
     1021 tgccggatca cccatagtta ttcctattat gctttttttc atgtttatca tccctttgtt
     1081 ttctcctaac atttattttg ctcaacaaaa ctccttattg cttcctgccc ttctatactt
     1141 atatggttaa aaggactcct acattctcct acattatgtc ccatcagatc agtagccttt
     1201 tttacaattg tattagggtt gccgtatcta aataattttc taaattctat tattttattt
     1261 tgattttcca gagctccttc ttggtcgcct tctttatatt tattgtatat atcagccatc
     1321 agactaggat atacattgga acatcccgca acagctccat caccgccagc ttgtaaggta
     1381 gataagatta gagcatcatt tcctgccaac actacaaaat cttctcttat ctcctttatt
     1441 tttatgtaag ccagtatatt taaaaagttc ccactgctat ccttgatccc cctaacattt
     1501 ggtaactttg ctagttttgc tgctgtttct gcttctatgt gatttcctgt tctagctggt
     1561 atattatata gaattatagg gatattaact gattctgcca ccaatttaaa gtgattatat
     1621 atttcttcct gtcctatctt ggcaaaatag ggtgttatga cagataaagc ttctaccccc
     1681 attttttctg cctccttaga taggcggata gtttcctcag tagttacaca gccggttcct
     1741 gcatatatag gaactctgcc cgccacctga tccacagtct ccctaattat gtttaccttt
     1801 tcttcaaagc ttagggcata ggcctctcca ttagttccaa ctggaaagat tccatggatc
     1861 ccaccttcta ttaatctgtc cacctccttt ctgagacctg tataattaat agtctcatct
     1921 gaattcattg gagttaaatt aggtgtaatt attccatgta tattcataaa aacactcctt
     1981 acataatttc aagatatatt tgttttatag tctctacatc gtattccttc atattattat
     2041 ttaagagcct ctttactcca aaggcagcct ctgctaaata atctaaatcc ttttcgtcaa
     2101 tattaaactc ttttaaagag gttggaattt ctaatattct tagcaattcc tccatccatt
     2161 gaagcacagc ttccgccttg ttttcactgc tatcccttcc ccatatatag tcatatacct
     2221 tttccagcct atcttcgatt acatccttgt tttctttcat tacaggtaat agaagcattg
     2281 cgttggaaag cccatgggcc attctatatt taccacctaa tgggtaggat agggcgtgta
     2341 ccgcagttgt acctgctgca gcaatagcca ctcctgcata atatgccgcc aaaagcatct
     2401 tctcttttgc cttcatatcc tcctttatat atgcctgttc tagattttca taaattaatt
     2461 ttaaagcctc caaactaaaa aggttgctta tatcattggc cttattggaa gtataacact
     2521 cgattgcatg gcatagagca tctaccccag tataagcaat aatcttttta ggtaactttt
     2581 ctat
//
LOCUS       DATGSI010000396         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_548088, whole genome shotgun sequence.
ACCESSION   DATGSI010000396 DATGSI010000000
VERSION     DATGSI010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548088"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VK031_06110"
     CDS             <1..351
                     /locus_tag="VK031_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53526.1"
                     /translation="AYRVLKVNSVYKLKDKIKLVINQVHKKEQAERAFDKLKITSEKF
                     LDIGLEKLGFIYNDTRINRSIMEQRPFVLKYPNALASQNIEQIAKNILGDKSYRYNVS
                     NVKEFKNRLLRFFG"
     gene            357..1025
                     /locus_tag="VK031_06115"
     CDS             357..1025
                     /locus_tag="VK031_06115"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HLS53527.1"
                     /translation="MENIRLNSKIELIAEDDSKIAGTVHDLVDGKIYVAVTAQDLKER
                     ILRPGEDVEAILYGDNEILSFEALISDRRFEDISLYEVSNISNLTKAQRRRDVRVPCS
                     ININYTDNKYLVNADFKKEELDRLLKEISNYLEEGIILDLSGGGLRLATRRNLEKGTR
                     LLLNFKLERELLLKGKIVHKEINVLPDKTNYFYGVKFIDIEEEIREEIIKYVFILMRK
                     QSLK"
     gene            1038..1760
                     /locus_tag="VK031_06120"
     CDS             1038..1760
                     /locus_tag="VK031_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HLS53528.1"
                     /translation="MGYDLTNQEELIIKYLPLVERIAGRIDTRDREYEREDLINIGVI
                     GLMDAIKRYDKNRKVPFEAYASLRIRGTIMDELRRNGKVSRDRIDKLNQYYSAKEDLE
                     NKLSRTPEESEIRDELGISEKELAKLHETLHYLSSTSLDTIIFAEKDSHIRLIDVIED
                     TETETPEEHFMRNERKRILAEAIEKLDYREKLILDLYYVEELTLREIAHILDISIPRV
                     SQIHGRVLIKLREIMEPKLEVV"
     gene            1760..2128
                     /locus_tag="VK031_06125"
     CDS             1760..2128
                     /locus_tag="VK031_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53529.1"
                     /translation="MVYFLVTLGLFLISLGLYLDYRKPLKVKKTQIDPALIDRIERLE
                     QVTFKALLEEEKNRENPSQAVEQLEIETSKEDRENGFFNKYKLLERYEREDKSLEEIC
                     QLLNMQKGEVFLLKNLYKDY"
     gene            2306..>2771
                     /locus_tag="VK031_06130"
     CDS             2306..>2771
                     /locus_tag="VK031_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HLS53530.1"
                     /translation="MIRSIYTANRHMNVLQTKQENTSANISNINTPGYKFQEIVQSTL
                     ESHQMINYSGGRRLNRRQELGEFVFGNQIDGFYRNFQQGNLSETNKATDFALVGSGFF
                     TIQMDNGEIAYTRNGNFRLNENNQLETMEGHLVLGVDENGNIGLIYGENNSVD"
BASE COUNT         1051 a          371 c          613 g          736 t
ORIGIN      
        1 gcatatcgag ttttaaaggt aaactcagtt tataaactaa aggataaaat aaagctggtg
       61 attaatcagg tccacaaaaa agagcaggct gaaagggcct ttgataagct taaaattact
      121 tcagaaaagt ttctagatat aggcttagag aaactaggct ttatatataa cgatactaga
      181 ataaataggt ccataatgga gcagcgtccc tttgtactaa aatatcctaa tgccctggcc
      241 agccagaaca ttgagcaaat agccaagaat attttgggtg ataaaagcta tagatataat
      301 gtgtccaatg tcaaggagtt taagaataga ctattaagat tcttcgggtg atccaaatgg
      361 aaaatataag gttaaatagt aaaattgaat taattgctga agatgatagc aagatagccg
      421 gcacagtcca tgatctggtg gatgggaaaa tatatgtggc tgtaactgcc caagacttaa
      481 aggaaaggat cttaagacct ggagaagatg tggaagcaat cctatatggt gataatgaaa
      541 tcctaagttt tgaggcccta atcagtgata gaaggtttga agatatctct ctttatgaag
      601 tatccaatat atcaaatcta accaaggccc aaaggagaag ggatgtaagg gttccctgtt
      661 ccataaatat taactataca gataataagt atttggtaaa tgctgatttt aaaaaggaag
      721 agctagatag gctattgaag gaaatttcca actatttgga ggaaggaata atcctagact
      781 taagtggcgg aggactaagg ctggctacta gaaggaattt agaaaagggc acaagacttt
      841 tacttaactt taagttagaa agggagttgc ttttaaaggg caaaatagtg cataaagaaa
      901 tcaatgtact accagataag actaattact tttatggggt taagtttata gatatagagg
      961 aagaaataag ggaagaaatt ataaaatatg tatttatttt aatgagaaaa caaagtctta
     1021 aatagaagag ggatttcatg ggttatgatt taactaatca agaggagcta ataattaaat
     1081 atttgcccct ggttgaaagg atagctggta ggattgatac cagggatagg gaatacgaaa
     1141 gggaagattt gattaatata ggagtcatcg gcttgatgga tgctataaaa aggtatgata
     1201 aaaataggaa ggttcccttt gaagcctacg cctccttaag aattagagga actattatgg
     1261 atgaattaag gagaaatggc aaggtaagcc gggataggat agacaagcta aatcaatatt
     1321 attcagccaa ggaagattta gaaaacaaat tatctagaac tccagaagaa agtgaaatca
     1381 gagatgagct aggcattagt gaaaaggaat tggccaaact acatgaaacc cttcattact
     1441 tgtcaagtac gtccctagat actattatat ttgcagaaaa ggacagccac atccgtctaa
     1501 tagatgtaat agaggataca gaaacagaaa caccagagga acactttatg agaaatgaga
     1561 gaaaaagaat tctggctgag gccatagaaa agctggatta tagggaaaaa ctcatattag
     1621 atctgtacta tgtggaggag ttaaccttaa gggagatagc ccacatattg gacatttcca
     1681 tacctcgggt atctcaaatc catggacggg tcctaattaa actaagggag ataatggagc
     1741 caaaattgga ggtagtgtaa tggtttattt tctggtaact ttaggccttt tcctaataag
     1801 tctaggccta tacttagact atagaaagcc cttaaaggtt aaaaaaacac aaatagatcc
     1861 agccctaatt gataggatag aaaggctgga gcaagtaact tttaaggccc tgctggaaga
     1921 ggaaaaaaat agagagaacc ctagtcaagc agtggaacag ttggaaattg aaacctctaa
     1981 agaagataga gaaaatggct tttttaacaa atataagcta ttagaaaggt atgaaaggga
     2041 agataagtct ttagaagaaa tttgccaatt acttaatatg caaaaagggg aggtattttt
     2101 attaaaaaac ttatacaaag attattaaaa gctgaaagta gctttcttat tccgttaggt
     2161 attggaataa ttataagtgc tctgctgatg catttaagct atagcttcaa ggtaaattat
     2221 aatattgaaa agaaggccag ggccatgggc atgatttatc ctgaagaggt cagggtaata
     2281 aatgaggaaa cgggggagtg aaaaaatgat tagaagcatc tatacagcaa ataggcatat
     2341 gaatgttttg cagactaaac aggaaaatac cagtgccaac atatccaata taaatactcc
     2401 aggttataaa ttccaggaaa tagtccaaag caccttagaa tctcaccaga tgattaatta
     2461 ttctggtggt agaagattaa atcgaaggca ggagttaggt gagttcgtct ttggaaatca
     2521 aatagatggt ttctatagaa actttcagca gggcaactta tctgaaacca ataaggctac
     2581 tgattttgcc ctggtgggca gtggattttt taccattcaa atggataatg gagagattgc
     2641 ctatactagg aatggaaatt tcagattaaa tgaaaacaat caactggaaa ccatggaagg
     2701 acatctggtc ctaggagtag atgaaaatgg taatataggc ctaatttatg gagaaaataa
     2761 ttctgtagat g
//
LOCUS       DATGSI010000397         3526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_259482, whole genome shotgun sequence.
ACCESSION   DATGSI010000397 DATGSI010000000
VERSION     DATGSI010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259482"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VK031_06135"
     CDS             <1..617
                     /locus_tag="VK031_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HLS53531.1"
                     /translation="PKPFAYKWTFNPTAIIAMLFMYLATAIETIGDISAITIGAEGRE
                     ATDEEMSGGVIADGLGSAFAALFNAFPNTSYTQNIGVISLTGVFSTSIVKIGAVILLV
                     LSLFPKFASLIAVIPEPVLGGASIAMFSIITVSGLSLLREVKLDSRNSLIIAIAVGLG
                     VGLNLVTEAVQLMPHSLQLFLTSGVGPSAIVAIVMDQLLPNRQD"
     gene            636..1589
                     /locus_tag="VK031_06140"
     CDS             636..1589
                     /locus_tag="VK031_06140"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HLS53532.1"
                     /translation="MKKERIIPELEAIILDRDFYKLLKSSNKSLGTIHSIYSKVINFT
                     NERSIYSIVSPFIDNGPSFLKVKNKDIDFTKLPIKVGEHVFKSGNLIHLSYGLTIRLS
                     EKIMIWDVGFKGRENINKDIIKKNISLFNKRILKEGKPGGTRYFYLNNWLKLDYRPLI
                     IEKELARRIENFLHAIDRDSLSQRELYSLIGCGMGLTPAGDDFLVGFISVLRFVQSAY
                     SKKLMKKITSLIYIDELSTTDISKHILGNALEGKFVESVPRLVNSLLEEDKYRLNISI
                     TNILEIGSSSGLDLSIGIVVALSLLMNNFNMEELEYEKYNC"
     gene            1570..3102
                     /gene="fdrA"
                     /locus_tag="VK031_06145"
     CDS             1570..3102
                     /gene="fdrA"
                     /locus_tag="VK031_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HLS53533.1"
                     /translation="MKNTIVRENAYYDSVTLMSLSSKVLGVEGVVEAVISMATDMNKE
                     LLENVGLATEESAQAGSNDLVIAIDAKDKESYEKAMDLVDELLSPKAQKKTRGEIQDP
                     KTIDKALEYMVDANMAVISVPGQYAAREAKKALNRDLHVMLFSDNVSLEEEKELKELG
                     REKGLLVMGPDCGTARINGVGLCFANEVRNGKVGIVGASGTGLQEVMVQIHRLGGGIS
                     QAIGTGGRDLSKEIGGIMMLEGLKALNKDDDTKVIVLISKPPAKSVEEKILREVEKTD
                     KPVVICFIDGDKSKKDHKNLYFVQGLFDAAKKSVELLRDEKIGGQDEVDFDLEALKAR
                     LKPEQKYLRGLFSGGTLCSEALSILREGLPGIKSNVAQDEKEELKDLKNYQGNVLLDL
                     GEDEFTQGRPHPMIEPALRLEKIVEEAKDKEVGVILMDFVLGFGSHEDPAGVTIESIR
                     KAKEIAREDGRELIFVAYICGTELDRQNYDRQKDILEAEGVIVADSNIKAAYMARELL
                     AR"
     gene            3113..>3526
                     /locus_tag="VK031_06150"
     CDS             3113..>3526
                     /locus_tag="VK031_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53534.1"
                     /translation="MGSINSLFSKDLKILNLGTSKFEEDLKEQNQEVIQLDWKPAAGG
                     DIELLKIIDRLAGNEEIVKANKEAVDRMINSHPILVDIDQAINVIPGMKENMILHSGP
                     PIQWERMSGPMRGAIIGALIYEGKAETEEEAVSLVE"
BASE COUNT         1215 a          488 c          826 g          997 t
ORIGIN      
        1 taccaaagcc ttttgcatat aaatggacat ttaacccaac agctataata gctatgttat
       61 ttatgtatct ggcaacggcc attgaaacaa taggagatat ttcagcaata acaattgggg
      121 ctgaaggtag ggaagctaca gatgaggaga tgagtggagg agttattgca gatgggctag
      181 gcagcgcttt tgcagcttta tttaatgctt ttcccaatac ttcctatact caaaatattg
      241 gggtaattag cttaactgga gttttcagta caagtatagt aaaaattgga gctgtaatct
      301 tattggtact gtctttattt ccaaaatttg cttccctgat tgcagtaatt cctgaaccag
      361 ttctaggcgg cgcttcaatt gccatgtttt caataataac agtttcaggt ctgtctcttt
      421 tgagggaagt taagttagat agcagaaata gcctaattat tgctattgct gtagggcttg
      481 gagttgggct taatctggta acagaggctg ttcaactaat gcctcatagt ttgcaactat
      541 ttttaacttc aggtgttgga ccatcggcca tcgtggcaat agttatggat caacttttac
      601 ctaatagaca agattaagat ggaagaaggg ctattatgaa aaaggaaaga atcataccag
      661 agttggaggc aattattctt gatcgggact tttataaact actaaagtct tctaataaat
      721 ccttaggtac catccatagt atttactcca aggtaataaa ttttaccaat gaaagatcca
      781 tctacagtat tgtatctccc tttatagata atggcccctc tttcttaaag gttaaaaata
      841 aggatattga ctttaccaag ctgcctataa aggtaggaga acatgttttt aaaagcggta
      901 atttaatcca tctatcttat gggcttacta taaggcttag tgaaaagatt atgatctggg
      961 atgtaggatt taaaggtagg gaaaatataa ataaagacat tataaagaaa aacatctctt
     1021 tatttaataa aaggatatta aaagaaggca agcctggtgg gaccagatat ttttatctta
     1081 ataattggct taagctggac tataggcctt tgataattga aaaagaactg gcaaggagaa
     1141 tagaaaattt tcttcatgcc atagatagag acagtttaag tcaaagagaa ctttattccc
     1201 taataggatg tgggatgggg ctgactccag caggagatga tttcttagtg ggatttattt
     1261 ctgtgctaag gtttgtccaa tcagcctatt ctaagaaatt aatgaagaaa attactagct
     1321 taatttatat agatgagctt tcaacaacag atattagtaa acatatactt ggaaatgcct
     1381 tagaaggtaa atttgttgaa agtgttccta gattagtaaa ttcattgctt gaggaagata
     1441 aatataggtt aaacatttcc attactaata tattggaaat tggttcttct tcaggcttag
     1501 atctatccat tgggatagtg gtggcactta gtttactaat gaataatttc aatatggagg
     1561 aattagaata tgaaaaatac aattgttaga gaaaatgcct attacgactc tgtaactctt
     1621 atgtctttat ctagcaaggt gttaggagtg gagggagttg tggaggcagt aatttccatg
     1681 gccacagata tgaataagga gctattggaa aatgtgggcc tggctacaga agagtctgcc
     1741 caagcaggta gcaatgacct ggttatagcc attgatgcta aagataagga aagttatgag
     1801 aaggctatgg acttggtaga tgaattacta agcccaaagg cccaaaaaaa gactaggggg
     1861 gaaatccaag atccaaagac tatagacaag gccctagagt atatggtaga tgccaatatg
     1921 gctgttatat cagtacctgg ccaatatgct gctagagagg ctaaaaaggc cttaaatagg
     1981 gatttacatg taatgctttt tagtgacaat gtcagtttag aagaggaaaa agaactgaaa
     2041 gaattgggta gggaaaaagg tctcctggtc atgggaccag attgtggtac tgccagaatc
     2101 aatggagtag gactttgctt tgccaatgaa gtaagaaatg gcaaggttgg aatagttgga
     2161 gcctccggta caggcttaca ggaggttatg gtccaaattc atagattagg cggagggata
     2221 tctcaggcca taggaactgg aggaagggat ttatcaaagg aaattggtgg aatcatgatg
     2281 ttggagggtt taaaggcctt gaataaggat gatgatacca aggtaatagt gctaatttct
     2341 aaaccgccag ctaaatcggt ggaggagaaa atcctaaggg aagtagaaaa aacagataag
     2401 ccggtagtta tttgctttat agatggtgat aaaagcaaaa aggaccataa aaatctttat
     2461 tttgtccaag gcctgtttga tgcagctaaa aaaagtgttg aattattaag ggatgaaaaa
     2521 ataggtggcc aagatgaagt tgattttgac ctggaggctt taaaggccag gttaaaacca
     2581 gaacaaaagt atttaagagg cttattttcc ggtggaacct tgtgtagtga agctttgtcc
     2641 atattaaggg aggggcttcc aggtataaaa agtaatgtgg cccaagatga aaaagaggaa
     2701 ttgaaggatc ttaaaaatta ccaaggtaac gtgttattag atttaggtga agatgaattt
     2761 actcagggca ggccccaccc tatgatagaa ccagccttaa ggttagaaaa gattgtagaa
     2821 gaagctaaag ataaggaagt tggagttatc ctaatggact ttgtactagg ttttggctcc
     2881 catgaagatc cggctggagt aaccatagaa tctattagaa aagcaaaaga aattgcccga
     2941 gaagatggaa gggaattaat ttttgtagct tatatatgtg gtactgaact agataggcaa
     3001 aattatgata ggcagaagga cattttagaa gctgaaggag tcatagtggc agatagcaac
     3061 ataaaggcag cttatatggc cagggaatta ctagcaagat aggaggatta tgatgggtag
     3121 tataaatagc ttattttcga aggatttaaa aatattaaac ttgggaacaa gtaaatttga
     3181 agaagattta aaggagcaaa atcaggaggt aatccaactt gattggaaac ctgctgcagg
     3241 tggagatata gaattattaa aaattattga cagactggct gggaatgagg aaattgtcaa
     3301 agctaataaa gaagcagtag ataggatgat aaattcccat cctattttag tagatataga
     3361 ccaggccata aatgtaatcc caggcatgaa ggaaaatatg atacttcatt ctggcccacc
     3421 aatccagtgg gaaagaatga gtggacctat gagaggggcc ataatagggg ctttaatata
     3481 cgaaggcaaa gctgagacag aagaggaagc agtaagcttg gtagaa
//
LOCUS       DATGSI010000398         1823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_217677, whole genome shotgun sequence.
ACCESSION   DATGSI010000398 DATGSI010000000
VERSION     DATGSI010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217677"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VK031_06155"
     CDS             complement(<1..284)
                     /locus_tag="VK031_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004816207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HLS53535.1"
                     /translation="MQELIQLFIVFFRMGAFTFGGGYAMLPIIQEEIVDKRGWATNEE
                     VIDYYAIGQCTPGIIAVNTATFVGYKRKGIMGGVFATLGLVSPSIIIITI"
     gene            505..726
                     /locus_tag="VK031_06160"
     CDS             505..726
                     /locus_tag="VK031_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HLS53536.1"
                     /translation="MPNNKSGSKRILVPEARQALNQMKLEIASELGMANYDSMDKGNL
                     TSRENGYVGGYITKNLVEMAQRNLGGTVR"
     gene            complement(768..1484)
                     /locus_tag="VK031_06165"
     CDS             complement(768..1484)
                     /locus_tag="VK031_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLS53537.1"
                     /translation="MRHKSLIGLLLILVLILISLGNYIQKIKMHRVSNIIDISKPDTK
                     PKYTIHIEVDKKRLSLIDRENEHVVKIYPIATGKASSPTPLGSFKIVDKAIWGEGFGS
                     RWLGLDIPWGIYGIHGTNEPGSIGRNVSAGCIRMFNDHIEELYNIVPHNTKVLISDGL
                     YGPFGSYFRTLRPGDRGADVLEVQKRLKQLGYYQADLDGIYGESMKRALVDFLKDEKM
                     DLTDKITEDIYERLGIILMD"
     gene            1633..>1823
                     /locus_tag="VK031_06170"
     CDS             1633..>1823
                     /locus_tag="VK031_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLS53538.1"
                     /translation="MEKYLEQLASSLLFKSIEKSKIPKILKTIPYTVKSYKKDEVIAV
                     EGEDCHSLGIILEGRVEIH"
BASE COUNT          629 a          309 c          257 g          628 t
ORIGIN      
        1 ataattgtaa tgattataat tgaaggggag accaatccta aggttgcaaa tactcctccc
       61 attatacctt ttcttttgta accaacaaaa gtggcagtat ttactgctat tatacctggc
      121 gtacattgac caatagcata ataatctatc acttcttcat tggtagccca acctctttta
      181 tcaactattt cttcctggat aataggtagc atggcatatc ctccaccaaa ggtaaaagca
      241 cccattcgaa aaaatactat aaataactga attaattctt gcaaatattt cacttcctat
      301 ttgaaaaatt aaactaccta tagattatac tatgagctat taaaaatgac aaattaatct
      361 aaaaatttta atatattact ttttttacct ttaattgaag aaggtgtatc tacttgataa
      421 atttgtctgg ttattaataa agcttttaca cataatacta atagacaaaa tttatatcaa
      481 aataaaatga aaggagatat gtaaatgcct aacaataaat ctggaagtaa aagaatatta
      541 gtaccagaag ctcgccaagc acttaaccaa atgaaactag aaattgcttc agaactaggt
      601 atggctaatt acgactctat ggataaagga aatttgacgt ctagagaaaa tggttatgtt
      661 ggtggatata taactaaaaa cttagttgaa atggcccaga gaaatctagg tggaacagtt
      721 agataacatt ttatataaaa gataggtcct aggacctatc tttcttttta atccattaat
      781 ataattccta acctttcata tatgtcttca gtaatcttat cagttaagtc cattttttca
      841 tcctttaaaa aatcaaccaa agcccttttc atggactctc cataaattcc atctaagtca
      901 gcttggtaat agcccagttg cttcagtctc ttttgtactt ctaatacatc tgcccctcta
      961 tctcctggtc ttagagttct aaagtatgac ccaaaaggtc catataaacc atcacttatt
     1021 aatactttag tattatgggg aactatatta tacaattcct ctatatggtc attaaacatt
     1081 cttatacaac cagcagacac atttctacca attgaccctg gctcattggt tccatgtatg
     1141 ccataaatac cccagggaat atctaaacct aaccatctag acccaaaacc ttcaccccaa
     1201 attgctttat ctacaatttt gaagctacct aagggtgtag gtgaggaggc tttgcctgtt
     1261 gctattgggt atatttttac cacatgctca ttttccctat ctattaggga tagtcgtttt
     1321 ttgtctactt ctatatgtat ggtatactta ggctttgtat caggttttga aatatctatt
     1381 atattagata ccctatgcat ttttatcttt tgaatgtaat tgcctaaact tattaatatt
     1441 aaaactaata taagtaataa acctattaaa gatttgtgcc tcatcctcat tccccctcat
     1501 actatttata tgataagata taaaaaataa aactttatta aatgcttggt caaaatttta
     1561 gtcttttagg ttgattgtag gctaatctgg tttgtgataa tcttaaatac tactaaaggt
     1621 agaggtgatt ttttggaaaa atatttggag caattggctt caagcctttt atttaaatct
     1681 atagaaaaat ctaaaatacc taaaatatta aaaactatac catatactgt taaatcttat
     1741 aaaaaagatg aggttatagc agttgaggga gaggattgtc atagtttagg aataatttta
     1801 gaaggcaggg tagagattca caa
//
LOCUS       DATGSI010000399         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_451475, whole genome shotgun sequence.
ACCESSION   DATGSI010000399 DATGSI010000000
VERSION     DATGSI010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451475"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..>1236
                     /locus_tag="VK031_06175"
     CDS             12..>1236
                     /locus_tag="VK031_06175"
                     /inference="COORDINATES: protein motif:HMM:NF037180.4"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HLS53539.1"
                     /translation="MYIEKKKDIDRERERLLKEFIHYLGIKSLKDLRVINVYDIIGVT
                     DEDSFKIIENILYEPQLDYLYMELPVLDKEELAFRVKYLEGQFNQREEAIKQLMKHLD
                     ISEDVEVFQSLILILKGIDMADLEKIKSYFINPVEMEEIGLDFSFKKRDITAVEEVEI
                     IEDFIRLNRGEMEKFKNEYGIGMDMDDILFCQEYFKKENKNPTITEIKLIDTYWSDHC
                     RHTTFNTEITDIRIHEGKYKEIFEKTLELYLSSRNYVYEDKEKPVCLMDLATINMKEA
                     WKKGVLDDREISDEVNAASIEIDVDVDGRVEKWLLMFKNETHNHPTEIEPFGGAGTCL
                     GGAIRDPLSGRSFVYQAMRISGSADPRKRFEATLAGKLPQRKISQDAMAGYSSYGNEI
                     GLATAYAREIYHQGFL"
BASE COUNT          477 a          147 c          274 g          338 t
ORIGIN      
        1 ataaaaaaag ggtgtatatt gaaaagaaga aagatataga cagggaaaga gaaaggttgc
       61 ttaaagagtt tatccattat ctaggtataa aaagcttaaa agatcttaga gtaataaatg
      121 tctacgacat aataggagtt acagatgaag atagttttaa gataatagaa aacatattgt
      181 atgagccaca gctggattat ttatatatgg aactaccagt cttagataag gaagagctgg
      241 cttttagggt taaatatttg gaaggtcaat ttaaccaaag ggaagaggca attaagcaac
      301 taatgaagca tttagatata agtgaagatg tggaagtctt tcaatcttta attttaattc
      361 taaagggaat agatatggct gatttggaaa aaataaaatc ctactttatc aatccagtgg
      421 agatggaaga gatagggctt gatttctcct ttaagaaaag ggatataact gctgtggaag
      481 aagtagaaat aatagaagac tttatacggc ttaatagagg ggaaatggaa aagtttaaaa
      541 atgaatatgg aataggtatg gatatggatg atattttatt ttgccaagaa tattttaaaa
      601 aagaaaataa aaatcccact attacagaaa taaaacttat agatacatat tggtcagacc
      661 attgcagaca tactaccttt aatactgaaa ttacagatat aaggattcat gaaggcaaat
      721 ataaagaaat atttgaaaag actttagaac tgtatttatc atctagaaat tatgtatatg
      781 aagataaaga aaagccagta tgtctaatgg atttggccac cataaatatg aaggaggctt
      841 ggaaaaaggg ggttcttgat gatagagaaa tatcagatga ggtaaatgca gccagtatag
      901 agatagatgt ggatgtagat ggtagggttg aaaaatggct gttaatgttt aaaaatgaaa
      961 cccacaacca tcctacagag atagagccct ttggaggtgc tggaacctgc ctaggaggag
     1021 ccattaggga tcccctatca ggcagatcct ttgtatacca ggccatgagg atttctgggt
     1081 cagcagatcc aagaaagaga tttgaagcaa cattagctgg gaagttacct caaagaaaaa
     1141 ttagccagga tgctatggct ggttattcat cctatggaaa tgagattggg ttagccacag
     1201 cctatgcaag agaaatttat caccaaggtt ttctag
//
LOCUS       DATGSI010000400         1440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_74702, whole genome shotgun sequence.
ACCESSION   DATGSI010000400 DATGSI010000000
VERSION     DATGSI010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74702"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..837)
                     /locus_tag="VK031_06180"
     CDS             complement(139..837)
                     /locus_tag="VK031_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53540.1"
                     /translation="MKNKKTLIGILFILAGISLVINRLGYFPNVNLFSVLISAYLVYV
                     IFKGVRSKSPFKILVPLSLIAVIYWDYIGPESLNPWTLVWSAILISIGISIIFRPTRE
                     FKFQKGIKYDENVDNFYKDEEGNIYIDTIFSESIRYLHGDDIRRVKLDSTFGSMKVYF
                     ENITMKDNHLLVDVDATFTGVELYIPREWDVVNDADVTFGSVNEKNRNASTATNTLLL
                     KGDITFGSVDIFYI"
     gene            complement(827..1294)
                     /locus_tag="VK031_06185"
     CDS             complement(827..1294)
                     /locus_tag="VK031_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLS53541.1"
                     /translation="MKITIQVDENINEEEIIIKCKDLRDDVYEIQNQISRILSKGKQI
                     VFYKDDTEYFIPLNDILFFETEGEGIFGHSREAMYQVRYRLYELEEILPFHFIRISKS
                     TILNVQHVKSINRNILSSSTVEFQKTHKKTYVSRHYYKDFKKRLMEVRGYYEK"
BASE COUNT          465 a          277 c          166 g          532 t
ORIGIN      
        1 ctttagctaa taattcctct tctgtcataa taacctccta agtatatata gactctcctt
       61 tttatttacc aaaaaaatat acaattaaac agaaaaatac ctagtatcta aaataccagg
      121 tatttaaatt ctcctatttt atatatagaa aatatctact gagccaaagg ttatatctcc
      181 ttttaatagc aaagtgtttg tagcagtact agcatttcta tttttttcat ttacagatcc
      241 aaaagttaca tcggcatcat taaccacatc ccactcccta ggtatataca actctacccc
      301 ggtaaaggtg gcatccacat ctactagtaa atgattatcc ttcattgtaa tattttcaaa
      361 ataaaccttc atggatccaa aggtgctatc taactttacc cttcttatat cgtctccatg
      421 cagatatcta atactttctg aaaatatagt atctatatag atatttccct cttcatcctt
      481 gtaaaaatta tctacatttt catcatattt aattcccttc tgaaatttaa attcccttgt
      541 cggcctaaaa ataatggaaa ttccaatact tattaaaata gctgaccaaa ctaaggtcca
      601 aggatttaag gactctggcc ctatataatc ccaatatatt acagctatta aggatagggg
      661 taccaggatt ttaaacgggc ttttgctcct tacaccttta aatattacat aaactaaata
      721 agctgatatt aaaacactaa acagattcac atttggaaaa tatcctaatc tatttataac
      781 cagactaatt cctgctaata taaacagtat ccctatcaag gttttcttat ttttcataat
      841 atcccctcac ttccattaat ctttttttga aatccttata ataatgccta gatacatagg
      901 tctttttgtg ggtcttttga aactctaccg tgctagatga cagtatattt ctattaattg
      961 attttacatg ttggacattt aatatggtgg atttggaaat ccggataaag tgaaagggta
     1021 gtatttcctc taactcatac agcctatacc ttacctgata catggcctcc ctactatggc
     1081 caaatatccc ttctccttct gtttcaaaga acaagatatc attaagcggt ataaaatatt
     1141 ctgtatcgtc cttgtaaaaa acaatttgtt ttccctttga gaggattctg gatatctggt
     1201 tttggatttc atatacatca tctcttaaat ctttacattt aattattatt tcttcttcat
     1261 ttatattttc atcaacctgg attgtaatct tcatcctctc accctttcat agataattat
     1321 atcctttaat ttacctttgt aaattctttt accctaagtg gtaaaaatat aagcctaagt
     1381 ggtaaaaatt ttaagatgaa aagtttacaa tttcctaatc ttgcaggtat gtagtattgt
//
LOCUS       DATGSI010000401         1089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_643114, whole genome shotgun sequence.
ACCESSION   DATGSI010000401 DATGSI010000000
VERSION     DATGSI010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643114"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VK031_06190"
     CDS             complement(<1..433)
                     /locus_tag="VK031_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS53542.1"
                     /translation="MIKVRNVTKTYGNKNVVDQVSVNIPKGKITSFIGPNGAGKSTLL
                     SMISRLIDKNSGEIIIEGKRIEDWDSKELAKKIAILRQSNHINIKLTVRELVSFCRFP
                     YSQGNLTEEDLKYINQAIGYMRLEDIQDRYLDQLSGGQRQRA"
     gene            complement(430..>1089)
                     /locus_tag="VK031_06195"
     CDS             complement(430..>1089)
                     /locus_tag="VK031_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HLS53543.1"
                     /translation="INKNINFVISTGLMVIFSWILYRVLFKKEKNNVVTLLLVGLILG
                     TLFQSISSFMQMMIDPNEFLHIQDRMFASFNNINTNILLISSLAILIIIIYTFRRSSI
                     LDVLSLGRENAINLGIDYDSTVFGLLLIVSILVSISTALVGPITFLGLLVVNLAREYL
                     KSYEHKYLILSSTLLSIIALIGGQLLVERIFNFGTPISVIINFLGGVYFIYLLIKENA
                     A"
BASE COUNT          363 a          204 c          146 g          376 t
ORIGIN      
        1 aggctctttg tctttgtccc ccgctaagtt gatctaagta tctatcttga atatcctcca
       61 ggcgcatata cccaattgcc tgattaatat attttaaatc ttcctcagtt aaattcccct
      121 gactataagg gaaacgacaa aagcttacta attcccttac ggtcaatttt atatttatat
      181 ggttggattg cctcaatatg gctatctttt tagccaattc cttgctgtcc caatcttcta
      241 tccttttccc ttcgataata atttcaccac tgtttttgtc aataagtctg cttatcatag
      301 ataagagggt acttttacca gctccattag ggcctataaa agaggttatc ttccccttag
      361 gtatattaac agaaacctgg tctaccacat ttttattacc ataggttttt gttacatttc
      421 taactttaat catgcagcat tctcctttat taatagatat ataaagtaaa ctcctccaag
      481 aaaattaatt attacgctta ttggtgttcc gaaattaaat attctttcaa ctaacaactg
      541 gccacctatc aaggctataa tgcttagcag ggtagatgat aaaattaagt atttatgttc
      601 ataacttttc aaatattccc tggccaaatt taccaccaat aagcctaaaa aggtaatggg
      661 accaaccagg gccgttgaaa tagatactaa gattgacaca attaataata aaccaaaaac
      721 agtactatca taatcaatgc caaggttaat ggcgttttct cttcccaatg aaagtacatc
      781 taatatacta gaccttctaa atgtgtatat aattatgata agaatagcca ggcttgaaat
      841 tagtagtatg tttgtgttta tgttgttaaa actggcaaac atcctatcct gaatgtgtaa
      901 aaattcatta ggatctatca tcatctgcat aaaggatgaa atactttgaa ataaagttcc
      961 aagaattagt ccaactaaaa gtaaggttac tacattattt ttctcctttt taaataaaac
     1021 tctatataaa atccaagaaa aaattaccat caagcccgtg gatatgacaa aatttatatt
     1081 tttatttat
//
LOCUS       DATGSI010000402         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_259877, whole genome shotgun sequence.
ACCESSION   DATGSI010000402 DATGSI010000000
VERSION     DATGSI010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259877"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /gene="fliR"
                     /locus_tag="VK031_06200"
     CDS             complement(<1..710)
                     /gene="fliR"
                     /locus_tag="VK031_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HLS53544.1"
                     /translation="MIIQLQKLILILVRITAFIVLCPGFSFRSLPNIMKVAISFSLTM
                     LVYFMTPEMQVTGSIPIFIILTVKEAIFGLAIGYISQLVFTAVEIAGRFIDFQVGFSM
                     GTVYDPNTGSNISNYGKLYYWLTVCVFFLLNLHHRLIETMIQSFNYIPITTVDLKNLS
                     IAGMIKLFGQVFEIGFNLAAPLIIVILLTDIILGIISRTIPQINVLLLGMPLKILVSM
                     VIILAVFSSLLNSIANTLG"
     gene            complement(719..985)
                     /gene="fliQ"
                     /locus_tag="VK031_06205"
     CDS             complement(719..985)
                     /gene="fliQ"
                     /locus_tag="VK031_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HLS53545.1"
                     /translation="MTQPMVLDIMRNTFITILKLAGPILIISLITGLIISILQATTQI
                     QEQTLSFVPKLLAVMLGLILLGSFMMNSLINFTSNLFNLIPTLG"
     gene            complement(996..1754)
                     /gene="fliP"
                     /locus_tag="VK031_06210"
     CDS             complement(996..1754)
                     /gene="fliP"
                     /locus_tag="VK031_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967730.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HLS53546.1"
                     /translation="MTKKLTLSITILILAVILIPNIALAQPELDGLGFIFNSEEGDPQ
                     AAVDSVKLFIILTVLSLAPAFLILTTCFTRIVVVLSFLRNGLGTQQSPPNQLLIGLAL
                     FLTFFIMKPVYTEIMDVSVTPFLEEEISQTEAMDRAAVPMKEFMLKFTREKDLALFLR
                     ASGSDNVEEPLDLPITTVIPAFAISELRTAFSIGFILFIPFLVIDMVVASILMSMGMF
                     MLPPVMISLPFKVLLFVLVDGWHLVIRSLVESFI"
     gene            complement(1751..>2101)
                     /locus_tag="VK031_06215"
     CDS             complement(1751..>2101)
                     /locus_tag="VK031_06215"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HLS53547.1"
                     /translation="LIKTLFLLILVIILAHLSLRALNKYMSKDGRLIRVIERVPVGNN
                     SYLAIVEICDSYYLMSLTPKDNRLLKELNKKDLENQLSKLELDRINATNISFLKEGRR
                     LIDSKISKWNEGKN"
BASE COUNT          692 a          397 c          363 g          649 t
ORIGIN      
        1 cctagggtgt tggctattga atttagaagg ctggaaaaca ctgccaagat aattaccata
       61 cttaccaata tttttaaagg catccccaat aataatacat ttatctgggg aatagttcta
      121 gatataatac ctaaaataat atctgtaagt aagataacta taattaaagg tgcagccagg
      181 ttaaaaccta tttcaaacac ctgaccaaat agttttatca tgcctgcaat gcttaggttt
      241 ttaagatcta cagtagttat aggtatatag ttaaatgatt gaatcattgt ctctatcagc
      301 ctatggtgta ggttcaagag aaagaaaaca catacggtta accaataata aagcttccca
      361 tagtttgata tattgctacc tgtattagga tcgtacactg ttcccatgga aaaacctacc
      421 tgaaagtcta taaacctgcc tgctatttcc acagccgtaa agactagttg actaatatag
      481 cctatggcca gtccaaatat ggcctccttt acagttaaaa taataaatat aggtatactg
      541 ccagttacct gcatttctgg agtcataaaa tacaccagca tggtcaggct aaaggatatg
      601 gccaccttca ttatattagg tagggatcta aaggaaaacc ctggacatag gactataaag
      661 gctgtaattc ttactaatat aaggattagt ttctgtaatt gaataatcat aatatctcct
      721 aacctagtgt aggtattaag ttaaataagt tactggtaaa atttattagg gagttcatca
      781 taaaactacc taataaaata aggcctaaca ttactgccag cagttttgga acaaagctta
      841 gggtctgttc ttggatctgg gttgttgcct gtaagatact tataataagc ccagttatca
      901 gggaaattat aagtattggg cctgccagct ttaatatggt tataaaggta tttctcatta
      961 tatctagtac cattggctgc gtcataaaat cacccctaga taaagctttc cactaaggaa
     1021 cgaattacta gatgccatcc atctaccagg acaaacaaaa gtaccttaaa tggtagggat
     1081 atcatcactg gaggtagcat aaacataccc atagacatta ggatactggc tactaccata
     1141 tctataacta aaaaaggaat aaaaaggata aagccaatac taaaggctgt cctcagctca
     1201 ctaatggcaa aggcagggat gactgtagtt attggtaaat ccaaaggttc ctccacattg
     1261 tcacttcctg atgcccttaa aaacagagcc agatcctttt ccctggtaaa ctttaacata
     1321 aactccttca ttggaactgc agccctatcc atggcctctg tttggctaat ctcctcttct
     1381 aaaaatgggg ttactgaaac atccattatt tctgtgtata ctggtttcat aataaagaag
     1441 gtaagaaaca aggccaaccc tattaatagc tggttaggtg gcgactgctg ggttcccagc
     1501 ccatttctta aaaaagatag gactaccacg attctagtaa aacaagtagt cagtattaaa
     1561 aaagctgggg ctaagcttag gactgtcagt attataaata gttttacact atctactgcc
     1621 gcctgaggat ctccctcttc cgaattaaat ataaagccta ggccatctag ctctggttgg
     1681 gccaaagcaa tatttggtat aagtattaca gctagaatta aaattgtaat ggaaagtgtt
     1741 aattttttag tcaattttta ccctcattcc acttgctaat tttactgtct ataagccttc
     1801 tcccctcttt aagaaaggaa atattggtgg catttatcct atccaattcc aatttggaca
     1861 gctgattttc taaatctttc ttgtttaatt cctttaggag cctattatcc ttgggagtaa
     1921 ggctcataag gtaataacta tcacatattt ccactatggc caggtaagaa ttgttgccta
     1981 ctggcactct ttcaattacc cttattaacc taccatcctt ggacatgtat ttatttaagg
     2041 ctcttagaga taggtgtgcc aaaatgatta ctaatattag taaaaacaaa gtttttataa
     2101 g
//
LOCUS       DATGSI010000403         1353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_26468, whole genome shotgun sequence.
ACCESSION   DATGSI010000403 DATGSI010000000
VERSION     DATGSI010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26468"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VK031_06220"
     CDS             <1..616
                     /locus_tag="VK031_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HLS53548.1"
                     /translation="DSKYKTIQEEYKIEGDWSSAAFFLAAASLGGIVRYSNLNIKSKQ
                     PDKIILKILNGFGSQIIFENDDIIVYPADFNNLDIDISQCPDLVPILAILATKTDSTC
                     YLRNGKRLRLKESDRLIATENMINSLGGKAEIRDDDLIIYGTNGLVGGEVDSYMDHRI
                     AMAAAIGSIVSREDIIIKNAQVVDKSYPNFFDDFKALGGIYNVI"
     gene            681..>1353
                     /locus_tag="VK031_06225"
     CDS             681..>1353
                     /locus_tag="VK031_06225"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HLS53549.1"
                     /translation="MENIPAGEFIDMDMLNNFLQRRAPGKSKYTTKRKEKDMPIFLSG
                     IKDNFTTGSPISAIIKNSDMKSKDYTNLENTFRPSHADFTSYYKYNNFNDKKGGGHFS
                     GRLTAPLCIAGGIIIQILERKNIYIGAHLLQIGNIKDKKFDDVNLSIDELRAPLSNNL
                     PVLSLDSQFKMQTLLDEVSKANDSIGASIECAVLNIPIGIGNPMFDGIENKISQYIFG
                     IPGVKG"
BASE COUNT          507 a          195 c          243 g          408 t
ORIGIN      
        1 agactcaaaa tataagacta ttcaagaaga gtataaaatt gaaggagatt ggtctagtgc
       61 agccttcttt ctagcagctg catcccttgg tggtattgtc agatattcaa acttaaatat
      121 aaagtcaaaa caacctgaca aaatcatatt aaagatatta aacggcttcg gctcccaaat
      181 tatatttgag aatgacgata taatagtcta tcctgccgat tttaacaatt tagatataga
      241 tatatcacaa tgtccggatt tagttcctat tttagctatt ttggccacta aaacagattc
      301 tacatgttat ctaaggaacg gaaaaagact tagactaaaa gaaagcgaca gactaatcgc
      361 aacggaaaat atgataaata gccttggtgg taaagctgaa atcagagacg atgatttaat
      421 aatctatggt acaaatggct tagtaggtgg agaagtagat tcttatatgg atcacagaat
      481 agcaatggct gcagctattg gttccatagt atcaagagaa gatataatta taaaaaatgc
      541 tcaagtagtc gataagtcat atcctaactt ctttgatgat tttaaagctt taggaggaat
      601 atataatgtc atctagtata ggcgataata taaaaataac tatatttggt gagtcccatg
      661 gtgaagctat aggtgttata ttggaaaata taccagctgg agaattcata gatatggata
      721 tgctaaataa tttcttacaa agaagagctc ctggaaaaag caaatatact acaaagagaa
      781 aagaaaaaga tatgcctata tttttatctg gcataaagga taattttaca acgggaagcc
      841 ctatctctgc aataattaaa aattcggata tgaaatcaaa agattatact aatttagaaa
      901 atacttttag accatctcat gcagatttta cctcttatta taaatataat aattttaacg
      961 ataaaaaagg tggaggacat ttctctggta ggttaactgc cccactttgc atagctggtg
     1021 gaattataat tcagatattg gagagaaaaa atatttatat aggcgcccac ctacttcaaa
     1081 taggaaatat aaaagataaa aaatttgacg atgtaaactt gagtatagat gaattacgag
     1141 ctcctttatc aaataatttg cccgttttat ctctggactc acagtttaaa atgcagacat
     1201 tattagatga ggtctctaaa gctaatgact ccataggtgc ctctatagaa tgtgcagttt
     1261 tgaatattcc tataggtata ggtaacccaa tgtttgacgg aatagagaac aaaatatctc
     1321 agtatatatt tggtatcccg ggtgtaaaag gca
//
LOCUS       DATGSI010000404         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_218120, whole genome shotgun sequence.
ACCESSION   DATGSI010000404 DATGSI010000000
VERSION     DATGSI010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218120"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VK031_06230"
     CDS             complement(<1..495)
                     /locus_tag="VK031_06230"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HLS53550.1"
                     /translation="MSRYTDKEMVKAAHYYYKQDLTQSQIAKRMGMSRQRVNRILKKA
                     VEENIVQITIVDVDKYNVELESKLEERFNLKEAVVVSVQEEGEVIPSLGQAGAEYLER
                     ILQKDDIMGVTWGRTLSEVAKRLPLNENLKVSVVQLIGGMNIAFTDLKADEITREMAR
                     KLGGE"
     gene            complement(1008..1667)
                     /locus_tag="VK031_06235"
     CDS             complement(1008..1667)
                     /locus_tag="VK031_06235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HLS53551.1"
                     /translation="MKLKDYGRVAILADFDGTITVRDTNVALIEKYVDPVAREKNSKK
                     RRKMNFTQGFQFLMNQVKVDKETYLDFILNEMELSDGFKEFYNHIQKKGIKFEVVSGG
                     FKDGIVPFLNKHGIKDVDIYSNRLIFGKDHLSIDFTDGEDMDCCQHGPCGNCKVRIYE
                     DFKKEYDQVIFIGDGVTDKPVAWIADMVFAKDSLKDYCIEHKIEHIAWNNFHDINKIL
                     F"
     gene            complement(1826..>2291)
                     /locus_tag="VK031_06240"
     CDS             complement(1826..>2291)
                     /locus_tag="VK031_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HLS53552.1"
                     /translation="ILDHRLRIDADQYIRLDDRSIPIEISKVKGTPFDFREAKAIRKD
                     LDLGHKDLKNPLGYDHPFILNKEQEGEILLSSPSSGVSLLVETTEPAVILYCSNKLEE
                     GMKLSGGEETFRYQGVCLETQWYPDAINQEFLPKNILRPDENYYSRTSYRFV"
BASE COUNT          673 a          492 c          282 g          844 t
ORIGIN      
        1 ttctcccccc agtttccttg ccatttccct agttatctca tcagccttta aatcagtaaa
       61 ggctatattc atacccccaa ttaactggac tacagaaacc tttaaatttt catttaaagg
      121 taacctttta gccacttctg ataaggttct cccccaggta actcccataa tgtcatcttt
      181 ttgcaagatt ctttctaagt actctgcccc tgcctggcct aggctaggaa ttacctcccc
      241 ctcttcctgg acagaaacta ctactgcctc ttttaaatta aacctttctt ctagcttgct
      301 ttccaattcc acattatact tatctacatc tactatggtt atttgaacta tgttttcttc
      361 tacagccttt tttaaaatcc tgtttactct ctgtcttgac atacccatcc ttttggctat
      421 ctgactttga gttaggtctt gtttgtaata ataatgggct gccttgacca tttccttatc
      481 tgtatatcta gacattttct actctccttg ttacatttgc tcagttagtg tttatttgtc
      541 atgttcttta acttgattat agcataaagc gtttataaag acaatatctt ctgaatattt
      601 tgttatgttt ttaaattact tattttttac attccatctt tctgtactat cctcgacttt
      661 atccatatac attttagcaa ttctatttaa agctttttat caatatttcc cgggaaaaat
      721 cgacaaaatc tcccaatata agtatcattt tttattgctt attgatttat aaatttgcaa
      781 cctaatataa taagtatttg tatctagttg tttttcgcca aattttttaa agattttcta
      841 aaaagcttat ttacttgttt ttattctaat ctatgctaat ttgactaaaa tcatcactgc
      901 aattgtacat ataatgtaaa gtaaggattt atttgtaact attgacaaaa cacttactta
      961 atacataata aaaacctact tccattcata atagaagtag gcacatacta aaaaagtatt
     1021 ttatttatat catgaaaatt attccaggct atatgctcaa tcttgtgttc aatacaatag
     1081 tcctttaagg aatccttggc aaataccatg tctgcgatcc aggcaactgg cttgtctgta
     1141 acaccatctc caataaagat tacctggtca tattccttct taaagtcctc atatatcctt
     1201 accttacaat tgccacaggg cccatgctga caacaatcca tatcctcccc atctgtaaag
     1261 tcaatggaca ggtgatcctt accaaaaatc agcctattgg agtaaatatc cacatctttt
     1321 atcccatgtt tgtttaggaa agggactata ccatctttaa atcctccact tactacttca
     1381 aacttaatcc ctttcttttg gatatggtta tagaattcct taaagccatc agataattcc
     1441 atctcattta aaataaaatc tagataagtc tctttatcca cctttacctg attcattaaa
     1501 aattgaaatc cttgggtaaa attcatcttt cgcctcttct ttgaattttt ctcccttgca
     1561 actggatcca catacttttc aattaaggcc acattagtat cccttacagt tatggttcca
     1621 tcaaagtcgg ccaatatggc gactctccca taatccttaa gtttcaatgt taatctcctt
     1681 tcttatgcta gtatattagt tttaccctaa ttagcattca tctaagagat tgtatcagga
     1741 aaagctggat aatgcaactt tgttaataaa gcatattata ttgacatatg taaagcatta
     1801 cccaaaatta taaatttaaa ttatactaaa caaaacgata acttgtccta ctataatagt
     1861 tttcatctgg tcttaatata tttttaggta ggaattcttg atttatagca tcaggatacc
     1921 attgggtttc taaacagact ccctgatacc taaaggtctc ttcgccacct gacaacttca
     1981 taccttcctc taatttattg ctacagtata atattaccgc cggctctgta gtttccacaa
     2041 gtagggatac accagaacta ggcgagctaa gtaaaatttc tccctcctgc tccttgttca
     2101 gaataaatgg atggtcatag cctaaaggat tttttaaatc cttatggccc aagtccaaat
     2161 cttttctaat agccttagcc tctctaaagt caaagggagt gcccttcacc ttactaatct
     2221 caatgggaat agatctatca tccagtctga tatattggtc agcatctatc cttagcctat
     2281 gatctaggat a
//
LOCUS       DATGSI010000405         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_125068, whole genome shotgun sequence.
ACCESSION   DATGSI010000405 DATGSI010000000
VERSION     DATGSI010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125068"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1598
                     /locus_tag="VK031_06245"
     CDS             297..1598
                     /locus_tag="VK031_06245"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HLS53553.1"
                     /translation="MEATRSKNVYSKQLLETLINGVKPALGCTEPVAVGIAVAKAHEV
                     VNGEVNDINVEVSPNIFKNGMGVGIPGTKEIGLVFASALAITCGDANLGLEVFKAVND
                     TAISQAEELVEKGLVNVELEDSKGNFYIRAKVKTDKGQGICVIRDNHTNIVYIEANGE
                     TLYESKEANTSDAHSNEYLKDVKLQDIREFVETVPYEDIKFLLEGVKLNMDMAKVGLD
                     EKPGPGLGAAMNLLMDEGVMKKDVINEARVLTSAACDARMAGINMPVMSSAGSGNHGI
                     TAIIPPTVVCEHIGCDDEKLARTLAFSHLITAYIKVFTGSLSPVCGCAVAAGIGASAS
                     IAWALGGTNEQIGGAIKNMVGTLTGMVCDGAKGGCAFKLSTASSEAIIQAKLAMSNIF
                     INDLDGIVSPEVEKTIRNLGSFSDKGMSSADQEIIKIMLNN"
     gene            1615..>1808
                     /locus_tag="VK031_06250"
     CDS             1615..>1808
                     /locus_tag="VK031_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HLS53554.1"
                     /translation="MSKLRDNLVVKLLIALIIGIVLGLVANESVIGVIQSIKHVVGQF
                     IFFCVPLVIIGFIAPSITGL"
BASE COUNT          642 a          248 c          403 g          515 t
ORIGIN      
        1 aaaattaaat ataggtctta ccaccctata tagaaaaatg aacgcttacg aaagctgatt
       61 cccacaaatg aaaattaatt tcccaattat gaaaaaagaa aaacataata taaataattt
      121 ttccctaaat tttatttatt aattttcact ttctgttaca aaaagcctgt aaacctgaaa
      181 agtaagccaa ttaataaaaa gaaatataat tctggatcga ttggcatgat acttgcaata
      241 tattttaggt gaaggcttac aacaatgaaa acattaacag aaaggaagat tagaaaatgg
      301 aagctactag atctaaaaat gtatattcta agcaattact agaaacttta ataaatggag
      361 taaaacctgc tttaggttgt actgagccgg tagctgtagg gattgcagtt gccaaggcgc
      421 atgaggtggt aaatggtgag gtaaatgata ttaatgtaga ggttagtcca aatatattca
      481 aaaacggtat gggggtaggc attcctggaa ccaaggaaat cggtttggta tttgcctctg
      541 ctttagctat tacatgtgga gatgccaact taggcttgga agtatttaag gctgtaaatg
      601 atactgcgat ttcacaagcg gaagaattgg ttgaaaaagg attagttaat gtagagttag
      661 aagacagcaa aggaaacttc tatattaggg ctaaggttaa aactgataag ggccaaggaa
      721 tatgtgtcat aagagacaat catacaaata tagtttatat agaggctaat ggggagactc
      781 tttatgaaag taaagaggcc aacacttctg atgcacatag taatgagtat ttaaaagatg
      841 taaagttgca ggatattagg gaatttgttg aaactgttcc ctatgaggac ataaagtttt
      901 tactagaagg agttaagctt aacatggata tggccaaggt aggccttgat gaaaaaccag
      961 gtcctggatt aggggcggcg atgaacctac taatggatga aggggtgatg aaaaaagacg
     1021 tcattaatga agccagagta ttaacttcag cagcttgtga tgccagaatg gcaggaatta
     1081 atatgccggt aatgagcagt gcaggaagtg gaaatcatgg tattacagct ataatcccgc
     1141 ctactgttgt gtgtgagcat attggatgtg atgatgaaaa actagctaga acactagcct
     1201 ttagtcatct aattactgcc tatataaaag tgtttacagg cagcctatca ccagtatgtg
     1261 gctgcgcagt ggctgctgga ataggtgcat ctgcatctat agcctgggct ctaggtggaa
     1321 ctaatgagca aattggggga gctattaaaa atatggtggg caccttaaca gggatggttt
     1381 gcgatggagc aaagggaggt tgtgcattta aattgtcaac agcttcatca gaagctatta
     1441 tccaggcaaa acttgccatg tctaatatat ttatcaatga tttagatggc atagtgagtc
     1501 cagaggtgga gaaaaccatt agaaatttag gaagtttttc agacaaagga atgagcagtg
     1561 cagaccaaga aatcataaaa ataatgttaa acaattaaaa ggggagatac aaaagtgtca
     1621 aaacttagag acaatttggt tgttaaactt ttaatagcac ttattatagg tatagtttta
     1681 ggtttagtag ccaacgaaag tgtgatagga gttatccaat caattaaaca cgtagtaggt
     1741 caatttatat ttttctgtgt tccactagta attattggtt ttatagcacc atcaattact
     1801 ggtttaaa
//
LOCUS       DATGSI010000406         1153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_125127, whole genome shotgun sequence.
ACCESSION   DATGSI010000406 DATGSI010000000
VERSION     DATGSI010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125127"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VK031_06255"
     CDS             <1..267
                     /locus_tag="VK031_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLS53555.1"
                     /translation="IFPLTLPGVITGITMVFIPAVSTFVISDLLGGNKYNLIGNIIEQ
                     QFRWTGDWHFGSSMSIILMVFILITMAITSSFDKEKEGGGGGLW"
     gene            261..1046
                     /locus_tag="VK031_06260"
     CDS             261..1046
                     /locus_tag="VK031_06260"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLS53556.1"
                     /translation="MVKKTLKKIYSYLIFLFLYAPIIVLIIFSFNNSKSRGVWAGFTF
                     RWYLELFQDAEVLRALYNTLLIAVLSSLISTIIGTFAALGIYSMKGFSKGLVLNLNYL
                     PVLNPDIVTAVSLMALYRFFKLDFGFLTMLLSHIVFSTPYVILSVLPKLKQMNIHLTE
                     AAMDLGATPFYALRKVIIPEIMPGIVTGGLLAFTLSIDDFVISFFNTGHGFSNLSIVI
                     YSMTRRGINPIINALSTLMFISLLILLLIINKRTDENIEQGGI"
BASE COUNT          373 a          206 c          201 g          373 t
ORIGIN      
        1 atcttcccct taacattgcc aggagtaata acaggtataa ccatggtatt tataccggca
       61 gtaagtacct ttgtaatatc agacctttta ggtggaaata agtataacct aataggaaat
      121 ataatagagc aacaatttag atggactgga gattggcact ttggttcttc catgtcaatt
      181 atcctcatgg tatttatcct aattaccatg gccataactt caagctttga taaagaaaag
      241 gaaggaggcg gcggaggatt atggtaaaaa agacacttaa aaaaatatac agctatttaa
      301 tctttttatt tctttatgca ccgatcatag ttttaataat tttttccttt aataattcaa
      361 aatcaagagg agtatgggca ggttttacct ttaggtggta tctagagctt ttccaagatg
      421 cagaggtctt aagagcctta tataacacct tgcttatagc tgttttatcc tctttaattt
      481 ccaccatcat aggcaccttt gctgccctgg gaatttatag tatgaaagga tttagtaaag
      541 gacttgtgct taatttaaac tatttgccag ttttaaatcc agatatagta acagccgtat
      601 ctctgatggc cttatatagg tttttcaaat tagacttcgg atttttgacc atgcttttat
      661 cccatatagt ttttagtaca ccctacgtaa tactatctgt cctacctaag ctaaaacaga
      721 tgaatattca cttaacagag gcggccatgg acctgggagc cacacccttt tatgccttaa
      781 gaaaggtaat aatcccagag attatgccag gaattgtaac cggtgggctt ttggccttta
      841 cactgtccat agacgacttt gtcataagtt ttttcaatac aggccatgga ttttccaatc
      901 taagtattgt gatctattcc atgaccagaa gagggataaa cccaataata aatgccttgt
      961 caacccttat gtttataagc cttttaatct tgcttttaat cataaacaaa aggacagacg
     1021 aaaacataga acaaggaggt atataattga aaagagtttt cttaatctta accataataa
     1081 ttttaggtat aagcctaaca gcctgtggcc aaaaaaaacc aaccattaac gtatataact
     1141 ggggggatta tat
//
LOCUS       DATGSI010000407         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_125128, whole genome shotgun sequence.
ACCESSION   DATGSI010000407 DATGSI010000000
VERSION     DATGSI010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125128"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="VK031_06265"
     CDS             <1..1424
                     /locus_tag="VK031_06265"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLS53557.1"
                     /translation="LSSEQLHTKKEKLKVTLFSIDEGVITTDKDGKVEMLNPKAESLT
                     GWTDLEAKGQPLNKVFNIYSEQSNRTYKSPALKVLEEKRPMRVRRDIILENKYGEKIN
                     ISISASPIKDKDGDVLGVVLVFRDITKIRQQEKRISDLSYRDSLTKSFNRAYLERAMD
                     RLDTEENLPISLVMGDLNGLKLLNDVFGHHVGDKALIEMSEILQKSCRSSDVVCRWGG
                     DEFVILLPKSSKQDVKKVCERVIHASEDLLIEDIRLSISLGYATKENIQESLSDTLKR
                     AEKNMYRRKLIDSQKHKELILSSLKDSFYKVDNKSRVQSEKLRRLAGEFGKFLSLPSQ
                     DIRDLELLAMVHDLGKASIDNTKIEDESLVAAEWDIINKHPEIGYRIASAIPELVGIA
                     EYILTHHERWDGKGHPRGLKGKDIPFLSRIFAVVDYYVAMTEKELLSPVESINELKKM
                     AHRQLDPEIVDKFIQYLKEKNRA"
BASE COUNT          532 a          227 c          321 g          428 t
ORIGIN      
        1 tactttctag tgaacaacta cacacaaaga aggaaaaatt gaaagtaacc cttttttcca
       61 tagatgaggg ggttattacc actgataagg atggaaaagt ggaaatgcta aatcctaagg
      121 cagaaagcct aaccggttgg actgacctag aggccaaggg ccaaccacta aataaggtgt
      181 ttaatatcta tagtgagcaa agtaatagga cctataaaag cccggccttg aaagttttag
      241 aagagaaaag gccaatgagg gttcgtaggg atattattct agagaataag tatggagaaa
      301 aaattaatat ttcaataagc gcttcgccta ttaaagataa agatggggat gttttaggtg
      361 tggtgctagt ctttagggat attactaaaa tccgccaaca ggaaaaaaga atcagtgatc
      421 taagttacag agattctctt accaaaagtt tcaatagggc ctatttagaa agggccatgg
      481 acagattgga tactgaagaa aatcttccca tatctcttgt tatgggtgat ctaaatggat
      541 tgaaactttt aaatgatgtt tttggccatc atgtgggaga taaggcctta atagagatga
      601 gtgaaatact tcaaaaatcc tgcagaagta gtgatgtggt ctgccggtgg ggtggggatg
      661 agtttgttat cctgctacct aaatcttcca agcaggatgt taaaaaagta tgtgaaagag
      721 ttattcatgc ctcggaagac ctattaattg aggatataag gcttagcatt tcccttggtt
      781 atgcaactaa agagaatata caagaaagct tatctgatac tctaaagagg gcagagaaaa
      841 atatgtatcg tagaaaactt attgattctc aaaaacataa ggagctaatc ctttcttccc
      901 taaaggattc tttctataag gtggataata aatcccgagt ccaatccgag aagttgagaa
      961 ggcttgctgg agaatttggg aaatttttaa gtcttccttc ccaagatatt agagatctag
     1021 agcttcttgc catggtccat gatctaggaa aagcatctat agataatacc aagatagaag
     1081 atgaatcttt agttgcagct gaatgggata ttataaataa acatcctgaa attggttaca
     1141 ggattgcttc agccatacca gaactagtag gtattgccga atatatattg acccatcatg
     1201 aaagatggga cggaaagggt catcctagag gccttaaggg caaggatatt ccttttttat
     1261 ctaggatctt tgcagttgta gactactatg tagctatgac tgaaaaggaa ttattaagtc
     1321 ctgttgaatc aataaatgaa cttaaaaaaa tggcacatcg ccaattagac cctgagatag
     1381 tagataaatt tatccaatat ttaaaggaaa aaaatcgggc ctagcccgat ttttatttat
     1441 aaggatttaa tttattagaa cttccataga agcaagatta aaggagtacc tataactaaa
     1501 attaatat
//
LOCUS       DATGSI010000408         1081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_171425, whole genome shotgun sequence.
ACCESSION   DATGSI010000408 DATGSI010000000
VERSION     DATGSI010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171425"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VK031_06270"
     CDS             <1..691
                     /locus_tag="VK031_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53558.1"
                     /translation="FVVINKPRTRKDGNTMKMSVTNVFREVYKASKHYNMKIDIVFID
                     PMNYFEKDGDEKFMQIQDYVLSIYEKVTEFSNLMDLHTIMSAHTRDVELKEDKELGIR
                     YYGILHPSEIVGGQSNYRAALQVLHLQRVPEGVLDKHGIPYPANYTVIHNQKVKPYGT
                     GKIGSTEKRGNKDGLYFDPDTHTMYEIIDGVKYYRNEYYNKDKEIQFKQNDSESAIKP
                     NLEFGLNDAPF"
     gene            713..859
                     /locus_tag="VK031_06275"
     CDS             713..859
                     /locus_tag="VK031_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53559.1"
                     /translation="MYEIINGCKYYRNEYYGTNGKNSSNNLESSTIKPNLDFGNSDDD
                     DLIF"
     gene            863..1048
                     /locus_tag="VK031_06280"
     CDS             863..1048
                     /locus_tag="VK031_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53560.1"
                     /translation="MSPEQMIMAIEVYIHLRTGETPDIDYRSILSSPEGMHYLMVAYG
                     YAEAWFQKNNVEIKYAN"
BASE COUNT          419 a          143 c          218 g          301 t
ORIGIN      
        1 ctttgttgta ataaataagc ctaggacaag gaaagatgga aacacaatga aaatgtctgt
       61 gactaatgtg ttcagagaag tttacaaagc ttctaaacat tacaacatga aaatagatat
      121 tgtattcatt gaccctatga actactttga gaaggatgga gatgagaagt ttatgcaaat
      181 acaggattac gtattatcta tttatgagaa agtaactgaa ttttctaact taatggattt
      241 gcacactatt atgtcagcac atactcgtga tgtagaactt aaagaagata aggaattagg
      301 gattaggtac tacggtatct tgcatccatc tgagattgta ggaggacaat ctaactatag
      361 agccgccctg caagtacttc atttacagag agttccagag ggagtgttag ataaacatgg
      421 tattccttac cctgcaaatt atacagttat tcataatcag aaagtgaagc cttatggtac
      481 aggtaagata gggagtacag agaaaagagg gaacaaagac ggtttgtatt ttgacccaga
      541 tactcacact atgtatgaga ttatagacgg agtaaaatat tataggaatg agtattataa
      601 caaagataaa gaaattcagt tcaaacaaaa tgatagtgaa tcagctatta aaccaaactt
      661 agaatttgga cttaatgatg caccttttta aacagataaa aactaattag atatgtacga
      721 gataataaac ggatgcaaat actacaggaa tgaatactat ggtacaaatg gtaaaaactc
      781 ttccaataat ttggaaagct caactataaa gcctaactta gattttggaa atagtgatga
      841 tgacgattta atattttaag atatgagccc agaacagatg ataatggcta ttgaggtgta
      901 catacactta cggactggag agaccccaga tatagattac agaagtatat tgagtagccc
      961 agaaggtatg cactacttaa tggtggcata tggatatgca gaagcttggt ttcaaaagaa
     1021 caatgtagag attaaatatg caaattagaa gagagttaat aaaatataag aagttatcgg
     1081 a
//
LOCUS       DATGSI010000409         1591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_451996, whole genome shotgun sequence.
ACCESSION   DATGSI010000409 DATGSI010000000
VERSION     DATGSI010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451996"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /gene="gatC"
                     /locus_tag="VK031_06285"
     CDS             <1..198
                     /gene="gatC"
                     /locus_tag="VK031_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HLS53561.1"
                     /translation="LEKLIAYVSKLDELDTEGVEPLIYLHPVENATRPDKLEKEFTQE
                     ELLRNAPTKEDGYILVPKIIE"
     gene            220..1542
                     /locus_tag="VK031_06290"
     CDS             220..1542
                     /locus_tag="VK031_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HLS53562.1"
                     /translation="MDLLDMDIAKLAKLLSSGEITSEKLTETYIENIEKSKVNYYETI
                     CKKQALAQAQRIDRQRKEGKQLGPLAGIPMAVADNITTKDILTTAGSKMLSSYIPPYN
                     ATVIERLSKEGAVLLGKLKIEEFNLEDSDVEAKAVSEKQAAYTLGTNIGGKATSIRPS
                     YGTVSRYGLIGASSSFEQIGVTGKNIRDLTIVLNTITGFDRRDSTSLPREKEDYLGFL
                     SQEPGKFKLFVANDFFKESINDKDKEKLSAILADLENIGAQVERVDLETIKYLHPAYK
                     ILASAEFASNAGRYDGIRFGYRAKDYEDREELYKNTRTEAFGHKAKLAILFGNYVVNG
                     SNYKKYYEKAQRVRRLIKDQVQELLADNNLLLIPQLKGQDEIESSYRLLAKMAGLPVI
                     NLPCKKDSLEGFKLQLLGPAFREEQLIQVGYALEKNIDKGSKELNIND"
BASE COUNT          605 a          244 c          341 g          401 t
ORIGIN      
        1 ttagaaaaac ttatagctta tgtatcaaaa ttagatgagt tagatacaga aggagtggag
       61 ccacttatat accttcatcc cgttgaaaat gcaactagac cagataagct agagaaggag
      121 tttactcaag aagaactctt aagaaatgct ccaactaaag aagacggcta tattttggta
      181 cctaagatta tagaataaaa gttataagga gggatcatct tggatttatt agatatggat
      241 attgctaaac tggctaaact cttgagttca ggagaaatta ccagtgaaaa attaactgaa
      301 acttatatag aaaacataga aaaaagtaag gttaactact acgaaactat ttgtaaaaaa
      361 caggccctag ctcaggctca aagaatagat agacaaagaa aagaaggtaa gcaactaggc
      421 cctttagcag gtatacctat ggctgtagca gacaacatta ctactaaaga tatattgact
      481 acagcaggca gcaaaatgtt aagtagctat ataccgccat acaatgcaac tgtaatagaa
      541 aggctatcaa aggaaggggc tgtgctctta gggaagctta aaatagaaga atttaacctg
      601 gaagattctg atgttgaggc taaggctgta tcagaaaaac aggcagcata tactctaggc
      661 accaatatag ggggcaaggc aacatctatc agaccaagct acggtacagt atccaggtat
      721 gggcttatag gggccagctc atcctttgaa caaattggag taacaggtaa aaatataaga
      781 gacttaacca ttgttttaaa tactataaca ggttttgata gaagggactc aacctcccta
      841 ccaagagaaa aggaagatta cttaggcttt ctttcacagg aaccagggaa atttaaactg
      901 tttgttgcca atgatttttt caaagaatca attaatgaca aagataagga aaaactttca
      961 gctattctag ctgatttgga gaatatagga gcacaggtgg aaagggtaga tttagaaaca
     1021 ataaaatatc tacatccagc ctataaaata ttggcaagtg cagaatttgc atcaaatgcc
     1081 ggtagatatg acggtattag atttggctat agggctaagg attatgaaga cagggaggag
     1141 ctctataaaa atacaaggac tgaggccttt ggtcataagg ccaaactagc catccttttt
     1201 gggaactatg tggtcaatgg tagcaactat aaaaagtatt atgaaaaggc ccaaagggtg
     1261 agaagattaa taaaagatca ggttcaggaa ttattggcag acaataatct acttttaata
     1321 ccacaattga agggccagga tgagatagaa tcatcctata ggctactggc caaaatggca
     1381 ggcctaccgg tgataaacct accttgcaaa aaagattctt tagaaggatt taagctacaa
     1441 ctattaggtc ctgcctttag ggaagaacag ctaatacagg taggatatgc tctagagaag
     1501 aatatagata aaggatctaa ggagttgaat ataaatgact aaggattttg aaactgttat
     1561 cggcgtagaa gtacatgtgg aattaaaaac a
//
LOCUS       DATGSI010000410         1019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_406516, whole genome shotgun sequence.
ACCESSION   DATGSI010000410 DATGSI010000000
VERSION     DATGSI010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406516"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VK031_06295"
     CDS             <1..303
                     /locus_tag="VK031_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53563.1"
                     /translation="TSYGASLPIGGMIKSNFYVEKLPMHKEVGVNPFSFEGRYRAWAA
                     FLEFGTGTYIDIPQGLTSYARTFIINGRGTILPSAFLFTATERQRAETMRKIKDRI"
     gene            300..710
                     /locus_tag="VK031_06300"
     CDS             300..710
                     /locus_tag="VK031_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53564.1"
                     /translation="MKLPDTEIRSAIISRLNGVISYPVRDMMSRPNDEPPYILLSTQT
                     SSQIPVKDRDFLESTILIQIIGSQNITVNRYEVEQITEDIMNELLPISPSNYIPLTDF
                     NIVSILLDSLNDDVITNDEGVTARKLLRLRLRLS"
     gene            748..>1019
                     /locus_tag="VK031_06305"
     CDS             748..>1019
                     /locus_tag="VK031_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53565.1"
                     /translation="MALRNTYNLKSFEADTAAEISDVVFKKNDMVLAIDTEETKKGDG
                     ETSFSDLPVFGSGGGSDAVAWSAITGKPSKFPPVVGTTASTAKAGN"
BASE COUNT          352 a          170 c          207 g          290 t
ORIGIN      
        1 acgtcttatg gagcatcctt acctattggt gggatgataa aatctaactt ttatgttgag
       61 aaattgccta tgcacaaaga ggtgggggtt aatcctttct cctttgaagg aagatatagg
      121 gcttgggcgg cttttttgga atttggcaca ggtacttata tcgatatacc tcaaggctta
      181 acctcttatg cacgaacatt tataataaat gggagaggta caatattacc aagtgctttt
      241 ttatttaccg ctacagaaag acaaagagcg gaaacgatga ggaaaataaa ggataggata
      301 tgaaattacc agatactgaa atacgaagcg cgataatatc gagattaaat ggggttatct
      361 cttatcctgt tcgggatatg atgtctcgcc ctaacgatga gccaccctac atattgcttt
      421 ctactcaaac cagttcccaa atacctgtaa aagacagaga cttcctagaa agcacaatat
      481 taatacagat tatagggtct caaaatataa ctgttaatag atatgaggtt gaacaaatta
      541 cggaggatat tatgaatgag ttgctgccaa tatctccttc taactacata ccattgacag
      601 attttaatat agtatctatc ttattagata gcttaaatga cgatgtaatt actaacgatg
      661 aaggcgtgac agctcgaaag ctattaagac taagattaag actttcgtaa gattaatata
      721 tttgtaaaag attaaaataa aaacgttatg gcactaagaa acacttacaa tttaaaatcg
      781 tttgaagctg atacagcggc tgaaatatcg gatgtagtat tcaaaaaaaa cgatatggta
      841 ttagctattg ataccgaaga aaccaaaaaa ggagacgggg aaacatcttt ttcagacctc
      901 cctgtattcg ggtctggagg tgggtcagat gctgttgcat ggagtgcaat aacagggaaa
      961 ccatccaaat tccctcctgt tgttggcact acagctagta cagctaaagc agggaatta
//
LOCUS       DATGSI010000411         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_549398, whole genome shotgun sequence.
ACCESSION   DATGSI010000411 DATGSI010000000
VERSION     DATGSI010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549398"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..606)
                     /locus_tag="VK031_06310"
     CDS             complement(127..606)
                     /locus_tag="VK031_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53566.1"
                     /translation="MVVAKSQLDYYPEEVQEKRRVEEERRKRKQKAIQRQRRLNVFLK
                     VATLMVVGIILSISLYILKGYANISQISMEINELEKQKKELEEIKASLDYDLIEIKSS
                     LKISEDAMYKLGMTYPRQDQIVYVSIDEGTEDNIGSENNQGISKKINEVVSLFSSFF"
     gene            complement(637..1569)
                     /gene="rsmH"
                     /locus_tag="VK031_06315"
     CDS             complement(637..1569)
                     /gene="rsmH"
                     /locus_tag="VK031_06315"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HLS53567.1"
                     /translation="MTFKHIPIMVDEVIKGLNIKKDGTYIDGTIGGAGHALEVTKRLD
                     KGLLVGIDQDKDALKKAEEVLSSHKDKVILVHSNYEKIDEILNNLNIDSVDGIFLDIG
                     VSSYQLDKAQRGFSYHQDAGLDMRMNKDQKFSAWDVVNKYSEKELSDIIWQYGEERWA
                     KRIAQFIVKEREKESIDTTGQLVEVIKKAIPKQVRMGGQHPARKTFQAIRIEVNRELE
                     VLENALRKMPNYLNPGGRLAVISFHSLEDRIVKQTFRYLFQDCICPPELPQCACDKER
                     EVKILTRRPITPSKREVEVNPRSRSAKLRIVEKL"
     gene            complement(1584..2015)
                     /gene="mraZ"
                     /locus_tag="VK031_06320"
     CDS             complement(1584..2015)
                     /gene="mraZ"
                     /locus_tag="VK031_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HLS53568.1"
                     /translation="MFIGEYQHTLDNKGRVTMPSKFRDDLGEKFVITKGLDSCLFVYP
                     MSEWKTIQEKLKTLPLTNRDARAFVRFFFSGASESSLDKQGRVLIPANLREHSKLEKE
                     AVIIGVATRLEIWSKDSWNDYTNDDNLSYDSIAEKMAELGI"
     gene            complement(2202..>2793)
                     /gene="lgt"
                     /locus_tag="VK031_06325"
     CDS             complement(2202..>2793)
                     /gene="lgt"
                     /locus_tag="VK031_06325"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HLS53569.1"
                     /translation="LLIFAIPAAIIGARLYYVAFSWDQYKDNPITALNFRNGGLAIHG
                     AIIAAIIVGVIFCRVRKQNFWLIADIAAPSLILGQAIGRWGNYINQEAYGRPTDLPWG
                     IMVDGVKVHPTFLYESLWNFLVFFFLIWYRRKKADVKGEIFLLYLILYSFIRYFVEGL
                     RTDSLMWGSIRVAQLISIVAIVIASAILYKKKKSRV"
BASE COUNT          792 a          619 c          380 g         1002 t
ORIGIN      
        1 tccctttatt aattgtaggt atcctagtct taatgttagt ccaaatacaa tcaaaagaga
       61 caatattaat gctgctctta gtctcattct tgatgatttg cttggtttat gcaaaatcaa
      121 aaccccctaa aagaaacttg aaaacaagct gaccacttca tttatttttt tgcttatgcc
      181 ttgattgttt tccgaaccaa tattatcttc agtaccttca tcaatagata cataaactat
      241 ttgatcttgg cgggggtagg tcatacctaa tttatacatg gcatcttcgc ttatctttag
      301 tgaagattta atttcaatta gatcgtaatc cagggaggcc ttgatttcct ccagctcttt
      361 cttttgcttt tccaattcat tgatctccat gctgatttgg ctaatatttg catagccttt
      421 taatatatat aaactaatag aaagaattat gccaactacc ataagtgtgg caacttttaa
      481 aaaaacatta agtcttcttt gcctttgaat agccttttgc tttctctttc ttctttcctc
      541 ttctactctt cttttctcct gtacctcctc aggataataa tctaattggg acttcgctac
      601 taccattttt attctccccc ttatcctttt agaactttaa agtttttcta caatccgtag
      661 tttagccgac ctggaccttg gatttacttc tacctctctt tttgaaggtg taattggcct
      721 tcttgttagt atctttactt ccctttcctt gtcacacgca cattgtggca attcaggtgg
      781 acatatacag tcttgaaata ggtatctaaa tgtttgctta acaatcctat cttctagaga
      841 atggaaacta attactgcca atctaccacc aggatttaag tagttaggca tctttctaag
      901 agcattttct aatacctcca attccctatt tacttctatc cttatggcct ggaaggtctt
      961 tctggcaggg tgttgtccac ccatccttac ctgttttggt atagcctttt ttatcacttc
     1021 caccaattgg cctgttgtat ctatactttc tttttccctt tcttttacaa taaactgggc
     1081 tattcttttg gcccatctct cctcaccata ctgccagatt atatctgata gttctttttc
     1141 agaatatttg tttaccacat cccaggctga aaatttctgg tccttattca tcctcatatc
     1201 taaacctgca tcctgatggt aagaaaatcc tctttgagcc ttatctaatt ggtaggatga
     1261 aactcctatg tctaaaaaaa ttccatctac agaatctata tttaaattat ttaatatttc
     1321 atctattttt tcataattac tatgaaccaa aattaccttg tccttatggg aagaaaggac
     1381 ttcctcagcc ttttttaaag catctttgtc ttgatcaatc cctactaaca aacccttatc
     1441 caatcttttt gtaacctcca gagcatgtcc agctccacct atggttccat ctatataagt
     1501 tccatctttc tttatattaa gacccttaat tacctcatct accataatag gtatatgttt
     1561 aaatgtcact taaatccctc cagctaaatt cctagttctg ccattttctc cgcaatactg
     1621 tcataactta aattgtcatc attagtataa tcattccagc tatccttact ccaaatttct
     1681 agtctagtgg ccacgcctat aattacagcc tccttttcta atttggaatg ttcccttaag
     1741 ttggccggga ttaatactct tccctgtttg tctaaagaag attcactggc gcctgaaaag
     1801 aaaaatctga cgaaggccct ggcatcccta ttggttaaag gaagggtctt taatttttct
     1861 tggattgttt tccactcaga catagggtat acaaaaaggc aactgtctaa acccttggtg
     1921 attacaaact tttcacctag gtcgtcacga aattttgaag gcatggtaac tcttccctta
     1981 ttatctaatg tatgctggta ttcaccgata aacatattta tcatcgcctt taatatatac
     2041 cactttgtcc cactttctcc cacattaatt atattctata ccattttcat aaaaccttcc
     2101 atatttatca tataatttaa gatatattta ggacctttct cctattttaa atctaatttt
     2161 gaccaaaaaa aaagaagcag ggaattccct gcttctttaa gttaaactcg tgattttttc
     2221 ttcttatata ggattgcaga tgcaataaca atagctacta tacttattag ctgggctact
     2281 ctaatagagc cccacattaa gctgtctgtt cttaagcctt ccacaaaata tctaatgaaa
     2341 gaatatagga ttaaatataa taaaaatatt tcgcccttca catctgcctt tttcctcctg
     2401 taccagatca ggaaaaaaaa tactaaaaag ttccacaaag attcatataa aaatgtggga
     2461 tgaaccttta caccatctac catgattccc caaggtaggt ctgttggcct accgtaggcc
     2521 tcctggttaa tataattacc ccagcgccct atagcttggc ctaaaatcaa actaggggct
     2581 gcaatatcgg caattagcca aaagttttgt tttctcaccc tgcaaaaaat taccccaact
     2641 ataatagctg caataatggc gccatggatt gccaggccac cgttcctaaa atttagggct
     2701 gttataggat tatccttata ttgatcccag ctaaatgcca cataatataa tctagctcct
     2761 attatggcag caggtatggc aaatattaaa aga
//
LOCUS       DATGSI010000412         1180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_358593, whole genome shotgun sequence.
ACCESSION   DATGSI010000412 DATGSI010000000
VERSION     DATGSI010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358593"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..>1180
                     /locus_tag="VK031_06330"
     CDS             64..>1180
                     /locus_tag="VK031_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010276737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLS53570.1"
                     /translation="MQRYILRSKNKITLISGILIAIAFINKWTTGNINIFVWSLVIAS
                     ILGATPIVIQAYQALRVKVVSIDVLVTIAVIGAFLIQNFEESAIVTFLFLFGAYLEQR
                     TLNQTRSAIKELTEMAPESALKQMEDGEFEEVEVDEVDVGDVLLVKTGAKVPVDGTVL
                     TGEGSINEASITGESIPVAKEKGSNVFAGTILDNGTIQIVADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKWYTPAVLVLAFIVWIFSRDVELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARHGVLLKGSEVISDFSRLDTMVFDKTGTLTVGNQSVAEKLYYGENIE
                     ETLGYLASIERESDHPLAKAVLEEIGEAEFSEVSDTEV"
BASE COUNT          496 a          148 c          257 g          279 t
ORIGIN      
        1 agaagtagac aaagcatatc aagagtaaag ataggagaag atatagttca aggaggggta
       61 agggtgcaga gatatatttt aagaagtaaa aataaaatta cattaataag tggtatctta
      121 atagccattg cctttataaa caaatggaca acaggaaata taaatatatt tgtttggtca
      181 ttagtgatag cctcaatatt aggtgcaaca ccaatagtca tccaagcata tcaagcatta
      241 agagtaaaag tagtaagtat agatgtatta gtaacaatag cagtaatagg agcatttttg
      301 atacaaaact ttgaagaatc agcaatagta acattcttat tcctatttgg agcatatcta
      361 gagcaaagaa cactaaatca aacacgttca gcaataaaag aattaacaga aatggcacca
      421 gaaagtgcat taaaacaaat ggaagatggg gaatttgaag aagtagaagt agacgaagta
      481 gatgtaggag atgtattact ggtaaagaca ggagcaaaag tgccagtaga cggaacagta
      541 ttaacaggag aaggcagtat caatgaagca agtataacag gggaatcaat accagtagct
      601 aaagaaaaag gatcaaatgt atttgcagga acaatcctag ataatggaac aatacaaata
      661 gtagcagaca gagtaggaga agatactacc tttggtaaaa ttatagaact tgtagaagaa
      721 gcccaagact caaaatcaga agcagaaaga tttatagata gattctcaaa atggtacaca
      781 ccagctgttt tagtgttagc atttatagta tggatattct caagggatgt agaactagca
      841 atcacaatcc tagtactagg ttgtccagga gcattagtaa taggagtacc agtatcaaat
      901 gtggcaggaa taggaaacgg agcacgtcat ggagttctac taaaaggtag tgaagtaata
      961 agcgatttta gtagattaga cacaatggta tttgataaga caggtacttt aacagtagga
     1021 aaccaatcag tagcagaaaa actatactat ggagaaaaca tagaagaaac attaggatat
     1081 ttagcaagca tagaaagaga atcagatcat ccactagcaa aagcagtatt agaagaaata
     1141 ggagaagcag agttttctga agtaagtgat acagaagtag
//
LOCUS       DATGSI010000413         1008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_27092, whole genome shotgun sequence.
ACCESSION   DATGSI010000413 DATGSI010000000
VERSION     DATGSI010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27092"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VK031_06335"
     CDS             <1..442
                     /locus_tag="VK031_06335"
                     /inference="COORDINATES: protein motif:HMM:NF016852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HLS53571.1"
                     /translation="AFVYHLFNREATSSNHFRFIGKMASLILRIIRKPETRYKIWKYA
                     ERQMSKYEIREDTKYLTDLQSVFRFMRLQYPKKYFHEAIYMEFEGHMMPVPKGYDGYL
                     KMAFGDYMKFPPKKERVPKHDASYINLEEGYKKFKGIHYCIDEK"
     gene            479..>1008
                     /locus_tag="VK031_06340"
     CDS             479..>1008
                     /locus_tag="VK031_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HLS53572.1"
                     /translation="MKKVITYGTYDLFHGGHLRLLQRAKKLGDYLIVGVTTDSYDESR
                     GKLNVQQSLMERIENVKSTGLADKIIIEEYEGQKVNDILKYDVDIFAIGSDWVGKFDY
                     LKEYCDVVYLERTKGISSTQLRIKNSGIIRMGIVGYGRIANRFIPESKFVSGVSVEGV
                     FGRNLEKLKDFKNRHQ"
BASE COUNT          385 a          125 c          224 g          274 t
ORIGIN      
        1 ggcctttgta tatcacctat ttaataggga ggctactagt tctaaccact tcagatttat
       61 cgggaaaatg gcttccctta tcttaagaat tataagaaaa ccagaaacaa gatataaaat
      121 atggaagtat gcagaaaggc aaatgagcaa gtatgaaata agagaagata caaagtattt
      181 aactgacctg cagtctgttt ttaggtttat gagattacag taccctaaaa agtatttcca
      241 tgaggccata tatatggaat ttgagggtca tatgatgccg gttcccaagg gatatgatgg
      301 gtatttaaaa atggcatttg gagactatat gaaatttcct cctaagaagg aaagagtccc
      361 taaacatgat gcctcataca ttaatttgga agaaggatat aaaaagttta aaggtattca
      421 ttattgtata gatgaaaaat agtgtcgcca tgttgattaa ctggaggaga ggataggtat
      481 gaaaaaggta ataacatatg gtacttacga tttgtttcac ggaggacatc taaggcttct
      541 acaacgggcg aaaaagctag gagattactt aatagtggga gtaactactg atagctatga
      601 tgaaagtaga ggaaaactaa acgtacaaca gagcctgatg gaaaggatag aaaatgtaaa
      661 atctacaggc ctagctgata aaataataat cgaagaatac gagggacaaa aagtcaacga
      721 tatcctaaaa tatgatgtag atatatttgc tattggttca gactgggtgg gaaaatttga
      781 ttacctaaaa gaatattgtg atgtagttta cctagaaaga accaaaggta tatccagtac
      841 tcagcttaga attaaaaata gtggaatcat aagaatgggt atagtaggct atgggagaat
      901 agccaataga tttatcccag agtcaaagtt tgtcagtgga gtaagtgtgg agggagtatt
      961 tggaagaaac ctggaaaaat taaaggattt taaaaataga catcaatt
//
LOCUS       DATGSI010000414         1832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_310085, whole genome shotgun sequence.
ACCESSION   DATGSI010000414 DATGSI010000000
VERSION     DATGSI010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310085"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VK031_06345"
     CDS             <1..697
                     /locus_tag="VK031_06345"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HLS53573.1"
                     /translation="LVQPVVDALHKMGVTGAETTGRNDIVVDGKKFSGNAMYTRGGKT
                     FSHGTLMFDVDTDAVADALRVPKDKIESKGIKSVRSRVTNIKPYLQRDMDILEFKEIL
                     LNHIFLMDGKEAEEYILNEEQQAEIEKLFKEKYSTWEWNYGSSPSFNYKNSKRFAFGL
                     IEIRLQIDQGYINQCKIFGDFFGSARIEDLEKALIGLRYDEEMVADFVEKVNLQSYFG
                     NIKNEEFIDLLFR"
     gene            1028..1441
                     /gene="glmS"
                     /locus_tag="VK031_06350"
     CDS             1028..1441
                     /gene="glmS"
                     /locus_tag="VK031_06350"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HLS53574.1"
                     /translation="MDKKTIVLGVIGSDCHAVGNRILDHSLTEAGFNVVNIGVLSPQE
                     DFINAAVETDADAILVSSLYGHGEIDCRGMREKCDEAGLEDILLYVGGNIVVGKQEWE
                     DVYKRFKDMGFDRVYGPGTPVDTAIKDLKEDLNIE"
     gene            1456..>1832
                     /locus_tag="VK031_06355"
     CDS             1456..>1832
                     /locus_tag="VK031_06355"
                     /inference="COORDINATES: protein motif:HMM:NF025309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HLS53575.1"
                     /translation="MDCYLFIDFGSTYTKVTLVDIEKEEILATAKAYTTVETDVTIGY
                     QKALDRLYKGIEHEVNIVKKLACSSAAGGLKIIAIGLVPELTAEAAKRAALGAGARVI
                     KTYSHNLNNSELEEIKSSEADMIL"
BASE COUNT          688 a          250 c          395 g          499 t
ORIGIN      
        1 tttagtacaa cctgttgtag atgctttaca taagatgggt gtaacaggtg cagaaacaac
       61 cggacgtaat gatattgtgg tcgatggtaa gaaattctct ggtaatgcaa tgtatacgcg
      121 tggtggcaaa actttctctc acggtacact aatgtttgat gttgatactg atgcagttgc
      181 tgatgcttta cgtgttccta aggataagat tgaatctaaa ggaattaagt ctgtaagaag
      241 ccgggtaact aatataaaac cctatctaca aagggatatg gacatactag aatttaaaga
      301 gattttacta aaccatatct ttctaatgga cgggaaagaa gcagaagaat atattttaaa
      361 tgaggagcaa caggcagaaa ttgaaaaact tttcaaggaa aaatactcca cctgggaatg
      421 gaactatgga agttcaccta gctttaacta taaaaatagt aaaagatttg cctttggcct
      481 aatagaaatt agactacaaa tagatcaagg atatataaac caatgtaaaa tatttggcga
      541 cttttttggc tcagcaagaa tagaagactt ggaaaaggcc cttataggtt taaggtatga
      601 cgaagaaatg gtggcagatt ttgtagaaaa agtaaattta caatcatatt ttggcaatat
      661 aaaaaatgaa gaatttatag atctactatt tagataaaaa aatggaccta gtaagtttta
      721 cttaatgggt ccattttaat ttaaaatggg aaaatgtttt caaaaaaata aattgtatta
      781 aaaaaagaac tattgcaaga taagaaaatg gagattgcaa ttctaacttc aataatctaa
      841 atttactgac aaataaaaac cataatgaaa gttgcaaatc cgatgccttc aaagtggctt
      901 aaaatagcaa tctagagaat tttattgggc aaaaataata cttgtcttta taaaatctta
      961 tgttattatt aaggtgaaga tagataaaaa taagacaaaa aataaggatt ttagggaggt
     1021 aaaggcaatg gataaaaaaa ccatagttct tggtgtaata ggttcagatt gccatgctgt
     1081 tggtaatagg atcctagatc actcattgac agaagctgga tttaatgtag taaatattgg
     1141 agttttgtct ccacaggagg atttcattaa cgcagctgta gaaacagatg cagatgcaat
     1201 cctagtttca tccctttacg gtcatgggga aatcgactgc aggggcatga gagaaaaatg
     1261 tgatgaggca ggcctggaag acatactgct atatgttggg ggaaatatag tggtaggtaa
     1321 gcaggagtgg gaggatgtct ataaaagatt taaggacatg ggctttgata gggtctatgg
     1381 acctggaaca ccagttgata ctgcaattaa agatttaaaa gaggacttaa atatagaata
     1441 aattgaggga ttaaaatgga ctgctatctt tttatcgact ttgggagtac ttatacaaag
     1501 gtaaccctag tagatataga aaaggaagaa atcctggcta cagctaaggc ctatactaca
     1561 gtggaaactg acgttaccat aggctatcaa aaagctttag acaggctata taagggaata
     1621 gagcatgaag taaatatagt caaaaaatta gcttgctcat ctgcagcagg aggcctaaaa
     1681 attatagcca taggcctggt accagagtta actgcagagg cagccaaaag ggcagccctg
     1741 ggggcaggag ctagagttat taaaacctac agtcacaacc taaacaacag tgaactggag
     1801 gaaataaaaa gctctgaagc agatatgatc ct
//
LOCUS       DATGSI010000415         1472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_171856, whole genome shotgun sequence.
ACCESSION   DATGSI010000415 DATGSI010000000
VERSION     DATGSI010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171856"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VK031_06360"
     CDS             complement(<1..287)
                     /locus_tag="VK031_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HLS53576.1"
                     /translation="MILQELREELISIGIQLKEYGLITLTGGNVSGRDPKTGLVAITP
                     SGMAYEALEAQDVVVVNLDGEIIDGERKPSSDLITHLQIYRAKEDINGIIHT"
     gene            complement(330..>1472)
                     /locus_tag="VK031_06365"
     CDS             complement(330..>1472)
                     /locus_tag="VK031_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HLS53577.1"
                     /translation="EMVARIGLELTVIDVGWPAAAAIAFASRVGALIIPLGLAVNIIM
                     LLTRTTQTVNIDIWNYWHFAFTGALVSGATGSFGLGLLAAALNMVIVMVMADLTAPGI
                     EESLGMPGISIPHGFAAAFVPIAIVINKILDFIPGINKIEIDAEGLQEKFGLFGEPLL
                     IGTILGILIGIGAGYDIQGILNTGIVLGAVLVLIPRMSSLLMEGLMPISDAAQELIQK
                     RYKGSGSLYIGLDAAIGVGHPVTLAVSLVLVPATILLAAIIPGNKLLPFADLAVIPFA
                     LVLLVPITKGDVFRTFIIGVVILSFGLLIATNIAPLHTQMAIDANFSMPEGATLISSI
                     CDGANPLSWSFAKVMDIKVIGAIIMTVIGIGMALYNRNRILKESGE"
BASE COUNT          441 a          327 c          235 g          469 t
ORIGIN      
        1 gtatggataa tcccatttat atcttcctta gctctatata tttgcaggtg ggtaattagg
       61 tccgaactag gctttctctc tccatctatt atttcaccat ctagatttac tacaaccaca
      121 tcctgtgctt ctaaggcttc ataagccata ccactaggtg taattgccac aagtcctgtt
      181 ttaggatccc ttccacttac atttccaccg gttaaagtaa ttagtccata ttctttcagc
      241 tgaattccta tggagataag ctcttctctt agctcttgta aaatcatgcc catcctcctt
      301 agctaacaca tctataggtt ttaattattt tattctccac tttctttaag aattctattc
      361 ctattgtata atgccatacc tatgccaata actgtcatga ttattgctcc tataaccttt
      421 atatccataa cttttgcaaa ggaccagcta agcggattgg caccgtcaca aatactagat
      481 attaaggtag caccctctgg catactgaag tttgcatcaa tagccatttg agtatgcaat
      541 ggagctatat tggtagctat taataaacca aaggatagga taactactcc tataataaag
      601 gttctaaata catctccctt ggttattggt accaatagca ccagggcaaa tggtataaca
      661 gctaagtcag caaatggtaa tagcttattt cctggaatta tagctgccaa tagtatggtt
      721 gctggtacta atactaaaga tacagccaag gttactggat gccctacacc aatagctgcg
      781 tctaaaccaa tgtatagtga gcctgaacct ttgtatcttt tctgaattaa ttcctgtgct
      841 gcatctgata taggcattag tccttccatt aacagagatg acattcttgg gatcagtact
      901 aaaactgctc ctaatactat acctgtattt agaatacctt gaatatcata tccagcccca
      961 atacctatta gtatacctaa aatggtacct ataaggagtg gttcaccgaa aagaccaaat
     1021 ttttcttgta agccctccgc atctatttct attttgttaa ttcctggtat gaagtctaaa
     1081 attttattaa ttacaatagc aattggtaca aaggctgcgg caaaaccatg tggtatagaa
     1141 attcctggca tacctaatga ctcttcaatt cctggtgcag ttaaatctgc cataaccatt
     1201 actataacca tgtttaaggc tgctgctaaa agtcctaagc caaagctacc tgtagcccct
     1261 gataccaaag caccagtaaa ggcaaaatgc cagtagttcc aaatatcaat gttaacagtt
     1321 tgcgttgttc tagttaatag cattataata tttacggcca agccaagtgg gattattaaa
     1381 gcccctaccc ttgatgcaaa ggcaattgct gccgctgctg gccaaccaac atcaattact
     1441 gtaagctcca ggcctattct tgcaaccatt tc
//
LOCUS       DATGSI010000416         1183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_27200, whole genome shotgun sequence.
ACCESSION   DATGSI010000416 DATGSI010000000
VERSION     DATGSI010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27200"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..705
                     /locus_tag="VK031_06370"
     CDS             130..705
                     /locus_tag="VK031_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004817860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HLS53578.1"
                     /translation="MAKVENVSYGKPLIAGAISTAPIGTELPTDATTKLDTKFKSLGY
                     ISEDGVTNNNSPESETIKAWGGDTVLVTQTDKPDTYTFTLIEVLNVDVVKVVYGDENV
                     SGNLSTGIEIKANSKPMQARVYVVESLLNNAIKRIVIPNGVITEVGDIAYKDDEPVGY
                     ETTVQCLPDEDGNTHYEFIKGSEEISTDEVS"
     gene            813..1010
                     /locus_tag="VK031_06375"
     CDS             813..1010
                     /locus_tag="VK031_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53579.1"
                     /translation="MEKVDSTGFGVSDLLNLMLGKKQKENLYKFCQEKDGKIPARKIF
                     DTIKEIMEDEKVKNSISLPES"
BASE COUNT          447 a          147 c          230 g          359 t
ORIGIN      
        1 ttttttggct aatgataaaa ggataagttc tgtaaagtta aactctgatt ataactttac
       61 tgatacagat atgaagaaat acagatatca agcagttttt gatatctatt attataaagg
      121 agagagaata tggcaaaagt agaaaatgta tcctatggta agcctcttat agctggtgct
      181 atatctactg caccaattgg aactgagcta cctacagatg ctactacaaa gttagataca
      241 aagtttaaat ccttaggtta tatttcagag gatggggtta ctaataataa cagtccagaa
      301 tctgaaacta ttaaggcttg gggtggagat actgttctag taactcaaac tgataagcct
      361 gatacctata cctttacgct aatagaggtt ttaaatgtag atgtagttaa ggtagtatat
      421 ggagatgaaa atgtatctgg aaatttaagt acaggcattg aaattaaggc taattctaag
      481 cctatgcaag ctagggtata tgtagttgag tctttactta ataatgccat taagaggatt
      541 gtaataccta atggtgttat tacagaagtt ggagatatag cttacaaaga tgatgagcca
      601 gtaggttatg aaactactgt gcaatgtctt cctgatgaag atggaaatac ccactatgaa
      661 tttattaagg gtagtgaaga aatatctact gatgaggtat cttaatgaaa aaaaataaaa
      721 agaaaaataa gtatgtaacc ggcatcacaa aggccggttt tagattttcc atagatccag
      781 aaataatgac agatatgaga tttgtagaat gaatggaaaa agtagattct actggatttg
      841 gtgtttctga tcttttaaat ttaatgcttg gaaaaaaaca gaaagagaac ttatataagt
      901 tttgccaaga aaaagatggt aaaattccag ctaggaaaat ctttgatact ataaaagaaa
      961 ttatggaaga tgaaaaagta aaaaactcta tatccttgcc ggaatcttaa aaaaacataa
     1021 aaatgaaatg atatgcgacc tagcccaatt ctacaatatt catgattatg aaagccttga
     1081 taaagtaagg ctttctattt atgtaaaagg actaccagaa gagtctaggg ttaaaagaaa
     1141 actttctggc cttaaatttg gcttagatac tattattagg gct
//
LOCUS       DATGSI010000417         1361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_500994, whole genome shotgun sequence.
ACCESSION   DATGSI010000417 DATGSI010000000
VERSION     DATGSI010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500994"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..869
                     /locus_tag="VK031_06380"
     CDS             195..869
                     /locus_tag="VK031_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HLS53580.1"
                     /translation="MLEKPMETNLVKIVGKTCTEKEYSHEMFGEKFYSIYVEIPRLSQ
                     SVDILPVTISERLLINLDLTLGQYVIIEGQLRSYNKYIDSANRLILTVFAREIYIPTE
                     EELEEAKRRPNEIFLDGYICKKPVYRVTPFGREITDLLVAVNRAYNKSDYIPCIAWGR
                     NARFCESLIVGDHIKLWGRIQSREYQKKLNDKEVENKVAYEVSISRLEFIEDDNNRVG
                     ADREEG"
     gene            1035..>1361
                     /locus_tag="VK031_06385"
     CDS             1035..>1361
                     /locus_tag="VK031_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HLS53581.1"
                     /translation="MEVDKRERILFSREEISERVKDLGQDISKAFQKKDLVVVSLLRG
                     SFIFAADLIREVSIPVEVDFITTSSYGHSESSSGQVDIVHDLRTTIEGRDVLIVDDIM
                     DSGYTMK"
BASE COUNT          516 a          182 c          285 g          378 t
ORIGIN      
        1 cagtattagc agcctcagcc ctaatccttg tcctaggcta taaatgctca catatatcct
       61 tataaagtaa tggaagttaa cttccattac tttttttcta tctaaccatt taacaaaatt
      121 attcataatc caatatttta atgaatactt atatatagat tggaaaatac agaggaatag
      181 aagggggcat atatatgtta gaaaaaccaa tggaaacaaa cctggtgaaa atagtgggaa
      241 aaacatgcac ggaaaaggag tatagccatg aaatgtttgg cgaaaagttt tatagtattt
      301 atgtggaaat acctaggctc agccagtcag tagatatttt acctgttaca atttcagaaa
      361 gattgttaat taatcttgac ttaaccctag gtcaatatgt tattattgaa ggacaattaa
      421 gatcctataa taaatatatt gactcagcaa ataggttaat cttaactgta tttgctagag
      481 aaatatatat tccaacggaa gaagaattgg aagaagctaa aagacggcct aatgaaatat
      541 ttttagatgg gtatatatgt aaaaagccag tctatagggt gactcccttt ggtagggaaa
      601 ttacagatct tttggtggca gtaaacaggg cctataataa atcagactat attccctgta
      661 tagcctgggg aagaaatgcc aggttttgtg agagcctaat agttggagat catataaagc
      721 tttggggtag aatccaaagt agagaatatc agaaaaaact aaacgacaag gaagtagaga
      781 acaaggtagc ctatgaagta tctatctcca gattagaatt tatagaagat gacaataata
      841 gggtaggtgc tgatagggag gaaggataga gaaatacatg gatacaaaat ttgtatttag
      901 tgttataata tttgtaatac tagtaagctt ccaatataca cttaacctta tactaaggga
      961 gataagggag attaaaaagc tgattgaaag gaagtttcga aaaccataac caaagatgaa
     1021 aggggagaaa aagcttggag gttgacaaaa gagaaagaat cctatttagc agagaagaaa
     1081 taagtgaaag agttaaggat ttaggacaag atattagtaa ggccttccag aagaaagatt
     1141 tagtagtggt gtcactatta aggggtagct ttatatttgc agcagactta ataagagaag
     1201 tatctatacc agtagaggtg gactttataa ccacctccag ctatggacat tcagaatcct
     1261 ccagtggaca ggtagatata gtacatgatt taaggactac cattgaaggc agagacgtac
     1321 ttatagttga cgatataatg gactcaggct ataccatgaa a
//
LOCUS       DATGSI010000418         1589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_644159, whole genome shotgun sequence.
ACCESSION   DATGSI010000418 DATGSI010000000
VERSION     DATGSI010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644159"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VK031_06390"
     CDS             <1..536
                     /locus_tag="VK031_06390"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="related protein from Bacillus subtilis functions as
                     methylribose kinase and functions in salvage pathway;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HLS53582.1"
                     /translation="HSGSIFVNEESTKIIDPEFAFYGPMGYDVGNVVGNLFFSWVNAY
                     VTMDEEEKREDFLSYLEGTIEDVVDLFTHKFKKMYKESVTDVLAKSPAYMDWYLNTVL
                     TDTCGVSGLEIMRRTVGDSKVVDITGIEDLQQRIKAERILILLAKYLILNRNHIRQGK
                     DYIDALQVALQEYNYHK"
     gene            549..1190
                     /locus_tag="VK031_06395"
     CDS             549..1190
                     /locus_tag="VK031_06395"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HLS53583.1"
                     /translation="MLLKKERERLVEYGKRLITRNLTTGSGGNLSIYNREKNLVAITP
                     SGVDYFDTKLEDIVVVDMEGNIVEGDLQPSSEIGMHLIFYKNREDANAIVHTHSKFAT
                     AIASMGWELEPVHYLIAFAGYDVKCAEYATYGTEKLAENALKAIEDRNAVLLANHGLI
                     ALGPDIGRAFSTAEHLEFVSEIYYLTKTLGEPKLLSRENIEEVMKKFNTFRYK"
     gene            1202..>1589
                     /locus_tag="VK031_06400"
     CDS             1202..>1589
                     /locus_tag="VK031_06400"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HLS53584.1"
                     /translation="MNSFSYYNPTGLVFGTDSVEELEKLLSGYEKVLLHYGGGSIKRT
                     GLYDQVISLLQKAGVDFVELAGVEPNPKLSLVRKGIDICKQEDIDMILAVGGGSVIDS
                     AKTIAAGSLYKGDIWECFTGAGSFKRA"
BASE COUNT          539 a          222 c          376 g          452 t
ORIGIN      
        1 tacattcagg ttcaatcttt gtaaatgagg agtctacaaa aattatagat cctgagttcg
       61 ccttttatgg acctatgggc tacgatgtgg ggaatgtggt gggtaattta tttttctcct
      121 gggtaaatgc ttatgtaacc atggatgaag aggaaaaaag ggaagatttt ttatcttatt
      181 tagaaggcac cattgaagat gtggtggact tatttaccca taagtttaaa aagatgtata
      241 aggagtcagt aactgatgtt ttggctaaaa gtccagctta tatggactgg tatttaaaca
      301 ctgtcttaac tgatacttgt ggtgtgtcgg gcctggagat tatgagaaga actgtgggag
      361 attcaaaggt agttgatata acaggtatag aagatttaca acaaaggata aaggcagaaa
      421 gaatactaat acttttagcc aagtatctta tcctcaatcg gaaccatata aggcagggta
      481 aagactatat agatgctctt caggtagcct tacaggagta taattatcat aaataaaggt
      541 gggtttatat gttattaaaa aaggaaaggg aaaggctagt agaatatgga aaaaggctta
      601 ttactagaaa tttaactact ggttcaggtg gaaacttaag tatctacaat agagaaaaga
      661 acttggtagc tattactccc agtggagtag attattttga cactaagcta gaggatatag
      721 tggtggtaga tatggagggc aatatagtgg aaggagattt gcaaccttcc agtgaaatag
      781 gtatgcactt gattttttat aaaaacaggg aagatgccaa tgccatagtc cacacccatt
      841 caaaatttgc tacagccatt gcctctatgg gctgggagct agagcctgta cactatctaa
      901 tagcttttgc aggatatgat gttaaatgtg ctgaatatgc cacctatggt acagaaaaat
      961 tggcagaaaa cgccttgaag gccatagagg ataggaatgc agttttattg gccaaccatg
     1021 gcctaatagc cttagggcca gatattggta gggccttttc caccgcagaa catttagagt
     1081 ttgtatcaga gatatactac ctaactaaaa ctttaggaga acctaagttg ttaagcagag
     1141 aaaatataga ggaagtaatg aaaaagttta atacttttag atataaatag gaggtgaaaa
     1201 aatgaatagc ttctcatact ataatcccac tgggctggtc tttgggactg actctgtgga
     1261 ggaacttgaa aaactattat caggctatga aaaagttttg ctccactatg gaggtggcag
     1321 tataaagagg acaggattat atgatcaggt tatatcttta ttacagaagg caggagtaga
     1381 ctttgtcgaa ttagctggag ttgaaccaaa tcctaaatta agcttagtaa gaaaaggaat
     1441 tgatatatgt aaacaggaag atatagacat gattctagct gttggaggag gtagcgtaat
     1501 agattccgcc aagaccatag cagccggtag cctatataaa ggagatatat gggaatgttt
     1561 cactggggca ggcagcttta aaagggctt
//
LOCUS       DATGSI010000419         1264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_690797, whole genome shotgun sequence.
ACCESSION   DATGSI010000419 DATGSI010000000
VERSION     DATGSI010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690797"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VK031_06405"
     CDS             complement(<1..284)
                     /locus_tag="VK031_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53585.1"
                     /translation="MRVSKPGASLQDAKRRLPLNIISNLTYVVISALFLLWLTPYLIE
                     HLGIELYGLIPLATAFVSYFNLVTVSISSSVGRYVIIHFQKADYHNSNVYL"
     gene            complement(281..>1264)
                     /locus_tag="VK031_06410"
     CDS             complement(281..>1264)
                     /locus_tag="VK031_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53586.1"
                     /translation="ENKSEEQIRAELKNIRSHGVNLVTLYSGKKNSEALDLYKEFGFS
                     DKGVLLIDFKMDSYVLKGDLGGYLKRAKELKRLISKKGIENLYIYGIDEANVNQISLQ
                     REFWSTVKESGVKVFSTGYSDTFPKMSSAIDIFAHAYKPSEEIEISAHEADVRVLSYA
                     NPQSGVENPERFRINYGMLLWAYNYDGSLVYAYQDAFGFIYNDFDHDVYRDHNLTYPT
                     SDGVIDTLAWKGYREGIYDLRYISTLESCIESDLLEGSENNSSAVISAVNYLNWLRDG
                     LKKEGSDKRTGDLGINGDEVRKQLVLHIENNCALDGERLVPPFNFKATVTE"
BASE COUNT          390 a          290 c          200 g          384 t
ORIGIN      
        1 agatagacat tactattgtg ataatcagcc ttctgaaaat gtataattac atatcttcca
       61 actgagctgg agatagaaac agtaaccaag ttaaagtaag aaacaaatgc cgtagctaat
      121 ggtatcagtc cataaagctc tatacccaag tgctcaatta gatagggagt cagccacagg
      181 agaaatagag cacttataac cacatatgtt aggttagata ttatattgag tggcagcctc
      241 cttttggcat cttgcaggct agcacctggc ttactgaccc tcattcagtc accgttgcct
      301 taaagttaaa aggagggacc agccgttctc catctaatgc acaattattc tctatgtgta
      361 aaaccaactg ctttctaact tcatccccat taatccctag gtcaccagtt cttttatcac
      421 taccttcttt tttcaaacca tctcttaacc agttcaaata attcactgcc gaaatgactg
      481 cagaactatt attttcacta ccctctaaaa gatctgattc tatacagctc tctagtgttg
      541 atatatacct aaggtcataa ataccttctc tatacccctt ccaagccaaa gtatctatga
      601 cgccatcact ggtcgggtat gtgagattgt gatctcggta aacatcatgg tcaaaatcat
      661 tataaataaa cccaaaagca tcttggtaag cataaacaag agagccatcg tagttatagg
      721 cccataaaag catcccatag ttgatcctaa acctctcagg gttctcaaca ccagactgtg
      781 ggttggcata gctgagaacc ctcacatctg cttcatgagc agatatttct atctcttcag
      841 aaggcttgta agcatgagcg aatatatcaa tcgcactgga catcttggga aaagtatcgc
      901 tgtatccagt tgaaaatact tttactccag actccttaac agtcgaccaa aactctctct
      961 gtagagaaat ctggttgaca ttggcttcgt caattccata tatatataaa ttttctatgc
     1021 ctttttttga aataagtctc ttaagctcct tagccctctt aagataacct ccaaggtcac
     1081 ctttaagaac ataagagtcc attttaaagt caatgagcaa aacaccctta tcagaaaaac
     1141 caaattcctt ataaagatcc agagcctcag agtttttctt tccgctgtag agagttacca
     1201 aattgacccc atggcttctt atatttttta gctcggctct aatctgttct tccgatttat
     1261 tttc
//
LOCUS       DATGSI010000420         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_219240, whole genome shotgun sequence.
ACCESSION   DATGSI010000420 DATGSI010000000
VERSION     DATGSI010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219240"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1309
                     /gene="obgE"
                     /locus_tag="VK031_06415"
     CDS             38..1309
                     /gene="obgE"
                     /locus_tag="VK031_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HLS53587.1"
                     /translation="MFVDKAKIFVKGGKGGNGAVAWRREKFEPSGGPFGGDGGDGGHV
                     IFKADEGIRTLMDFRYKRSYSGENGEDGRNKRQFGKAGEDVIVKVPVGTLVKDEKTGR
                     VIVDLREKGQEYIIAKGGKGGRGNARFATATRQAPGFAEAGTKGEERHVILELKLIAD
                     VGLIGFPNVGKSTILSVLSAARPKIANYHFTTLKPNLGVVRVGDEKSFVLADIPGLIE
                     GASEGVGLGHSFLRHVERTRVLIHVLDASGIEGRDPIKDFHTIMDELIKYNPKLKDKP
                     QIIVGNKMDLPGAQEGIERIKEEFEKEGYQVLDLSAATNQGIEKLKYATWDLLSKTEV
                     TYDTFDEEYIEEIKEEEETIIVKKENGQYIVEGSFIERLLESTYFDNVESLRYFQTTL
                     RRRGVVERLEELGIEEGDTVLICGYEFEFFH"
     gene            1347..1640
                     /gene="yhbY"
                     /locus_tag="VK031_06420"
     CDS             1347..1640
                     /gene="yhbY"
                     /locus_tag="VK031_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HLS53588.1"
                     /translation="MLTGKQRSYLKGLANKIDPIFQLGKLGLSENFIKQLDTALESRE
                     LIKINVLKNSFHDPKEVANELAEKLDAEFVQSIGNKFVLYRESEENKKIQLPD"
     gene            1663..2262
                     /gene="nadD"
                     /locus_tag="VK031_06425"
     CDS             1663..2262
                     /gene="nadD"
                     /locus_tag="VK031_06425"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HLS53589.1"
                     /translation="MKIGILGGTFNPIHHGHLILSEYIREEARLDKIIFIPTGISPHK
                     DQEEVLNKHDRKEMLKLALASNPNYKISTIEIDRQGKSYTIDTIKKLKNIYPKDDLYM
                     IIGADSLLELNTWKDYEDIISSIKIIVMDRQVSCREKLKEKITYYNKTYQGQIKRIET
                     PIIEISATEIRRRIKRGKSIKYLLPELVEDYIIKNKLYR"
BASE COUNT          858 a          349 c          526 g          536 t
ORIGIN      
        1 gatatactgg ccagtatgga ttatggggat gataaatatg tttgtagaca aggccaagat
       61 ctttgtcaaa ggtggcaaag gtggtaatgg ggcagtagcc tggagaagag aaaagtttga
      121 gccttcagga ggcccctttg gtggagatgg tggagacgga ggccatgtaa tctttaaggc
      181 tgatgaaggt ataaggacat tgatggactt tagatataaa agatcctatt caggagaaaa
      241 tggagaagac ggtagaaata aaaggcaatt tggtaaagca ggtgaagatg taatagtgaa
      301 ggtgccagta ggtaccttag taaaggatga aaaaacaggc agggttatag tagatttaag
      361 ggaaaagggc caagaatata taatagccaa gggcggcaag ggtggtagag gtaatgccag
      421 atttgccaca gccaccaggc aggcaccagg ctttgcagaa gctggaacca agggagaaga
      481 aagacatgta atcctagaat taaagctaat agcagatgtg ggccttatag gctttcccaa
      541 tgtaggtaaa tctaccatac tgtctgtcct atcagcagcc aggcctaaaa tagccaatta
      601 tcatttcact accttaaaac caaacctggg agtagttagg gtaggagatg aaaaaagctt
      661 tgtcctagca gacataccag gcctaataga aggagcctca gaaggagttg gcctgggcca
      721 tagcttttta aggcatgtgg aaagaaccag ggtcctaatc catgtactgg atgcttctgg
      781 aatagaagga agggatccaa ttaaggactt tcacaccatc atggatgaac tgataaaata
      841 caatcctaag ctaaaggata agcctcaaat tatagtaggc aataaaatgg acctacctgg
      901 agcccaggaa ggaatagaaa gaataaaaga agaatttgaa aaagaaggct atcaggtact
      961 ggacctgtca gcagccacca accagggtat agaaaaacta aaatatgcta cttgggacct
     1021 gctatctaag acagaagtaa cctatgatac ctttgatgaa gaatatatag aagagataaa
     1081 agaagaggaa gagacaatta tagtgaaaaa ggaaaatggt caatatatag tggaaggtag
     1141 cttcatagaa aggctgctgg aatcaaccta ctttgacaat gtggaatcct taagatattt
     1201 tcaaactacc ctaagaagaa ggggagttgt agaaagatta gaggaactgg gcatagaaga
     1261 aggagataca gtccttatat gcggttatga atttgaattt ttccattaat tatatctact
     1321 tttaaaaggc aaaggaaggt gataaaatgt taacaggtaa acaaagatct tacctaaagg
     1381 gtttggccaa caagatagac ccaatctttc aattgggcaa gctgggctta agtgaaaact
     1441 ttattaaaca gctggacaca gccttggagt ccagggaact gataaagata aatgtcttaa
     1501 aaaacagctt ccatgaccct aaagaagtgg ccaatgaact ggcagaaaaa ctagatgcag
     1561 aatttgtcca aagtatagga aataaatttg ttttatatag agagtctgaa gaaaacaaaa
     1621 agattcagct accagactaa tcttacctaa ggaggtgtag gtatgaaaat tggtattcta
     1681 ggtgggactt ttaaccctat ccaccatggc cacctgatct taagcgaata tattagagag
     1741 gaggccaggc tggataagat aatctttatt cccactggaa tttcacctca caaggaccag
     1801 gaggaagtat taaataaaca tgacagaaaa gagatgctaa agctggccct agcatcaaat
     1861 cctaattata aaatatctac catagaaata gatagacaag gaaaatccta taccatagat
     1921 acaataaaaa aattaaagaa tatttatcca aaggatgacc tatatatgat aataggtgca
     1981 gattctttac tggagttaaa tacctggaag gactatgaag atattatatc ctccataaag
     2041 attatagtta tggataggca ggtatcctgt agagaaaagt taaaggagaa aatcacctat
     2101 tacaataaaa cctaccaggg ccagataaaa aggattgaaa cacctattat tgaaatatct
     2161 gccacagaaa taagaaggcg gattaaaaga ggcaagtcta ttaagtacct attacctgaa
     2221 ctggtggaag actatattat aaaaaacaag ctatatagat gataggtgg
//
LOCUS       DATGSI010000421         1283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_76342, whole genome shotgun sequence.
ACCESSION   DATGSI010000421 DATGSI010000000
VERSION     DATGSI010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76342"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VK031_06430"
     CDS             <1..1028
                     /locus_tag="VK031_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53590.1"
                     /translation="SKNYFRVSERKNKILDSRDKNLSRGYDIGFYSAHDYFNVKKGTT
                     SYHYAFPFSGKTSYLFDILVYMAKEHGIKIAIYSPETGDDTALVTFIVQVYLGKKLHG
                     RGAQTATDEEWLEALDFIDNHFIFLDPVMVGENKVTFSAKEMFKQVYNAQKEYGWKVE
                     VVVADPYNLLARDEEDRRKSIAEYTLDNLSYINQVAVSMDIHVMIAMHLRDEEAIVDK
                     ETGVEYMSRPFPNKIANGQSVWRAGQIMVGLWRCPAGVIEKSTGIPYPENRTDFLVQK
                     NKMLGTGETGTFSLYYDKDRQKFYEIIDGVKYYCGEYKQKAKEQVGESALKPNTDFGV
                     AENNLPF"
     gene            1042..1242
                     /locus_tag="VK031_06435"
     CDS             1042..1242
                     /locus_tag="VK031_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53591.1"
                     /translation="MNLEEMIYEVESYIKARKGVDVTINPQLVMGSPYGPMLLQYAYQ
                     VAIAWKEENEKSKQGKSESSNK"
BASE COUNT          462 a          174 c          309 g          338 t
ORIGIN      
        1 tgagtaagaa ttattttaga gtatcagaga ggaagaataa gatattagat tcaagggata
       61 agaatttatc gagaggatat gacatagggt tttattcagc ccatgactac tttaatgtaa
      121 agaaaggtac gacttcatac cattatgctt ttccattttc gggcaaaact tcatacctat
      181 ttgatatact ggtatatatg gctaaggagc atggaattaa gatagcaatc tatagcccag
      241 aaacaggtga tgatactgca ttagtgacct tcatagtcca agtatattta ggtaagaagt
      301 tgcatggtag aggggcgcag acagcaacag acgaagagtg gttagaagct ttagacttta
      361 tagataatca ctttatattc ttagaccctg taatggtagg agagaataag gtaacttttt
      421 cagctaaaga gatgtttaag caggtatata atgctcaaaa ggaatacgga tggaaggtag
      481 aggttgttgt agcagaccct tataacttat tagctaggga tgaagaggac aggaggaaat
      541 ctatagcaga atatacctta gacaacttat cctatattaa tcaggtagca gtcagtatgg
      601 atattcacgt gatgattgct atgcatttaa gagatgaaga agcgatagta gataaggaaa
      661 caggagtaga gtacatgagc aggccttttc ccaacaaaat cgccaacggg cagtcagtat
      721 ggcgtgcagg gcagattatg gtgggcttat ggagatgtcc agcaggggtt atagagaagt
      781 ctacaggtat tccttaccct gaaaatagaa cagacttctt ggtccagaag aataagatgt
      841 taggaactgg ggaaacaggt acattctcct tatattacga caaagatagg cagaagttct
      901 atgagataat agatggagtt aagtactatt gtggagagta taaacagaag gctaaggagc
      961 aggtaggaga gtcagcactt aaaccgaata cagatttcgg agtagctgag aacaatttac
     1021 cattttaaaa atgaaaatac tatgaattta gaagaaatga tatatgaggt agagagttac
     1081 ataaaggcta ggaaaggagt cgatgtaact attaatccac aattagtaat gggtagtcca
     1141 tacggaccta tgttgttaca atatgcttat caagtagcta tagcatggaa ggaggaaaat
     1201 gaaaaaagta agcagggaaa gtctgaatca tctaataagt agagatgcta ccctaattaa
     1261 atacgaggac ttccgaaagg agt
//
LOCUS       DATGSI010000422         1329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_407397, whole genome shotgun sequence.
ACCESSION   DATGSI010000422 DATGSI010000000
VERSION     DATGSI010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407397"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /gene="pstA"
                     /locus_tag="VK031_06440"
     CDS             complement(<1..767)
                     /gene="pstA"
                     /locus_tag="VK031_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HLS53592.1"
                     /translation="MDRVLKSLVHIGAVISFGFVLLIIGYVGFKGIGHIDLDLFSLHY
                     TTENLSLLPSLITTFIIGLISLLVAIPIGVFSAIYMVEYANNQSFLIKVIRLATETLA
                     GIPSIVYGLFGLLFFVTYLNWGFSLLAGSVTLAIMVLPLIIRTTEEALLSVDNSLREA
                     SYALGVSKVRTIFKIVLPTALPGIFSGIVLAFGRIMGESAALIYTAGTVPNLPKGIFS
                     SGRTLTIHMYSLITEGINRDKAYATAVVLLVFVLVIN"
     gene            complement(769..>1329)
                     /gene="pstC"
                     /locus_tag="VK031_06445"
     CDS             complement(769..>1329)
                     /gene="pstC"
                     /locus_tag="VK031_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HLS53593.1"
                     /translation="KESMYKFLKPIVDLMAGIPSIIYGFFGLMVIVPVIRDVFGGTGH
                     SILTASILLGIMILPTIINLTENALKSVPSTYYQGAVALGVSKEIAIFKVIIPAAKQG
                     ILSAIVLGIGRAIGETMAVVMVAGNQPHLPNGVLDGVRTLTTNMIMKMGYASGVHKEA
                     LIATGVFLFLFTLLLNIILSVLKRRL"
BASE COUNT          469 a          263 c          171 g          426 t
ORIGIN      
        1 aagtttatca ctaatacaaa aactaaaaga acaacagccg tagcataagc tttatctcta
       61 tttattcctt ctgttatcaa actatacatg tgtattgtaa gcgttcttcc tgatgagaaa
      121 attccctttg gtaaatttgg aactgttcca gctgtataaa ttaaagctgc cgactcgccc
      181 attatcctac caaaagctaa tactattcca gaaaaaattc ctggtaaggc tgtaggtaaa
      241 actattttaa atatcgttct aactttactt acacctagag cataacttgc ttctcttagc
      301 gaattatcta cgcttaataa agcttcttct gtagttctta ttattaaagg taaaaccata
      361 atagccaaag ttaccgaacc tgctaatagt gaaaaacccc aatttaaata agttacaaaa
      421 aacaataaac caaaaagtcc ataaactata gatggtatac ctgctaatgt ctctgttgca
      481 agtcttataa ccttaattaa aaagctttga ttgtttgcat attctaccat atatattgca
      541 gaaaatactc ctatcggtat agctactaaa agacttatca aacctattat aaatgtcgtt
      601 atcaacgaag gtaataaaga tagattttca gtagtataat gtaagctaaa taagtctaaa
      661 tcaatatgtc ctatgccttt aaagcctaca taccctataa ttaaaagtac aaatccaaaa
      721 cttataacag cccctatatg aactaaggat tttaaaactc tatccatctt acaacctcct
      781 ctttagtaca cttaaaataa tatttagtaa aagagtaaac aaaaataaaa atactcctgt
      841 agctatcaat gcttctttat gaactcctga agcatatccc attttcatta tcatatttgt
      901 cgtaagagtt ctaactccat ctaatacgcc atttggcaga tgaggttgat ttccagctac
      961 cattaccaca gccatagtct cgcctatcgc tctacctata cctagaacaa tagctgaaag
     1021 tataccttgt tttgcagccg gtattataac tttaaatatc gcaatttcct tgctaacccc
     1081 taaggcaaca gccccttggt aataagttga aggtactgat ttcaaagcat tttctgttaa
     1141 gtttattata gttggaagta tcattatacc aaggagtata gatgccgtaa gtatgctatg
     1201 tcctgtacca ccaaagacat ctctaataac tggtactata accataagtc caaaaaaacc
     1261 gtagattata gaaggtatcc ctgccattaa atcaactatc ggttttagaa atttatacat
     1321 actttcttt
//
LOCUS       DATGSI010000423         1124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_219396, whole genome shotgun sequence.
ACCESSION   DATGSI010000423 DATGSI010000000
VERSION     DATGSI010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219396"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /gene="truA"
                     /locus_tag="VK031_06450"
     CDS             <1..629
                     /gene="truA"
                     /locus_tag="VK031_06450"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HLS53594.1"
                     /translation="YDIIREEVKLTGSGRTDAGVHALGQVANFKLENAIPVDKIPNAL
                     NSKLPKDISIIDCVEVNDEFHSRYNAKGKTYRYLIHNSMYRSPIYKDISYHVKYELDF
                     DKMVKESKYLIGTHDFKGFMSSGSSVKDTVREIYDIRLSKQEDLIVLEVEGNGFLYNM
                     VRIIVGTLVDIGRGRINKCLKEIVESKSRSECGHTAPAHGLFLKKVDY"
     gene            749..>1124
                     /gene="rplM"
                     /locus_tag="VK031_06455"
     CDS             749..>1124
                     /gene="rplM"
                     /locus_tag="VK031_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HLS53595.1"
                     /translation="MKSYIAKPADVQRKWYIVDAEGKTLGRLATEIATVLRGKHKVTF
                     TPHVDGGDFVVVVNAEKVVLTGKKLDQKVYRSHSGYIGGMKETTARDLLNKNPEQVML
                     RAVKGMLPHNSLGRQMLKKLRVY"
BASE COUNT          439 a          123 c          238 g          324 t
ORIGIN      
        1 tttatgatat aataagagaa gaagttaagc ttacaggttc tggtaggact gatgcaggag
       61 ttcatgcatt aggacaagtt gctaatttta aattagaaaa tgctatacct gtagataaaa
      121 taccaaatgc attaaactct aagttaccta aggatatttc tataattgac tgtgtagaag
      181 ttaatgatga atttcactct agatataatg caaaaggaaa aacatatagg tacttaatac
      241 ataatagtat gtatagaagt cctatatata aagatatatc atatcatgtt aagtatgaac
      301 ttgattttga taagatggtt aaagagtcta agtatttaat aggaacacat gattttaaag
      361 gatttatgag ttcaggatcg tctgtaaaag atacagtaag ggaaatatat gacataagat
      421 taagtaagca agaggacctt attgtattag aagtagaagg gaatggtttc ttatataata
      481 tggtaaggat aatagtaggc acactggtag atataggtcg aggaagaata aataagtgct
      541 taaaagaaat agtggaatct aaaagtagaa gtgagtgtgg tcatactgca ccagcacatg
      601 gattatttct taaaaaagtt gattattagc ttgacacacc agggtgcatg tattaaaatg
      661 aattagagtg tgttaataag tccacttgcc ccggacttga cataaacggt aaaattttta
      721 tatgaaaaaa agttaaggag ggacaactat gaaaagttat atagctaagc cagctgatgt
      781 acaaagaaaa tggtacatcg ttgatgctga aggaaaaaca ttaggtcgtt tagcaactga
      841 aatagcgaca gtattaagag ggaagcacaa agttactttc actccacatg ttgacggagg
      901 ggacttcgtt gttgttgtaa acgctgagaa agtagtttta acaggaaaga aattagatca
      961 aaaggtttat agaagccatt caggatatat tggtggtatg aaagaaacaa cagccagaga
     1021 tttattaaat aagaatcctg aacaagtaat gttacgtgct gtaaaaggaa tgttacctca
     1081 caatagttta ggaagacaaa tgttaaagaa attaagagta tatg
//
LOCUS       DATGSI010000424         1849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_501573, whole genome shotgun sequence.
ACCESSION   DATGSI010000424 DATGSI010000000
VERSION     DATGSI010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501573"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VK031_06460"
     CDS             complement(<1..397)
                     /locus_tag="VK031_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HLS53596.1"
                     /translation="MKLSRKLIFAFVTLIILSIMVISIISNIMINNRFENYLISEREE
                     KFQRIYYEINQLYIDNNYKLDPMELIHYALAEDINIIIKDTSNKIIYNSNTRRSMGMM
                     GGMHHGHMGHMGYMHRPEGKYIEKEYPLTF"
     gene            509..847
                     /locus_tag="VK031_06465"
     CDS             509..847
                     /locus_tag="VK031_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53597.1"
                     /translation="MKRLLIIISILAVIAIGSAFVYADSPDRNRFFLTKDSTGEWQGW
                     HKERMDEHRHRLNRAVDEGNITKEEAEAWEEHFDYMEDFHKENGFMRGRWGCHGRGGH
                     GMMRRYKRGW"
     gene            869..1000
                     /locus_tag="VK031_06470"
     CDS             869..1000
                     /locus_tag="VK031_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004834766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LDCC motif putative metal-binding protein"
                     /protein_id="HLS53598.1"
                     /translation="MIRTWIKDFLERLIASNKKEFGNKRLDCCEIKKMQIRKSRLVK"
     gene            complement(1011..1700)
                     /locus_tag="VK031_06475"
     CDS             complement(1011..1700)
                     /locus_tag="VK031_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLS53599.1"
                     /translation="MSHDCILVVEDEKNISDVIKAYLEKEGYKVITAYDGQEAMDLFA
                     KYKPDLIILDLMIPKIPGEKVCSMIRNTSQVPIIMLTAKSHEEDKIDGLSLGADDYMV
                     KPFSPRELVSRVKALMRRSYRDGGILAERLVFSNGLEIYTDKFQVIKNKQDVNLTTNE
                     FKIFIILASNPGIVFTREQLIDRAFGYDYDGFHRTIDTHIKNIRQKIEDNPKEPKYIL
                     TVYGVGYKFEG"
BASE COUNT          612 a          307 c          301 g          629 t
ORIGIN      
        1 caaaggttag ggggtattct ttttctatat atttcccttc aggcctatgc atatacccca
       61 tatgacccat atgaccatgg tgcattccac ccatcattcc catactccgc ctagtatttg
      121 aattatatat aattttgtta ctggtatctt taataataat atttatatct tctgctaagg
      181 cataatggat aagctccata ggatctaatt tataattatt atcgatatat aactggttaa
      241 tctcataata tattctttga aacttttctt ctctttcgct gatcaaatag ttttcgaatc
      301 tattgttaat cattatattg gaaattatac ttataaccat gatagaaaga ataattaagg
      361 taacaaaggc aaagataagc tttctagata gtttcatttt tttcctccaa actattattt
      421 attttaaatt atatcaaaaa aaacatatat cttcatagtt cctccacatt tatatcttac
      481 aataaaatca aatgaaggag ggtatgaaat gaaaagactt ttaattatca tatctatatt
      541 ggcagtaata gccattggct ctgcctttgt gtatgctgat agccctgata ggaatagatt
      601 tttcttaact aaggattcta ccggagaatg gcaaggctgg cataaggaaa gaatggatga
      661 acacaggcac cggttgaata gggcagtaga tgaaggtaac attactaaag aagaagcaga
      721 agcctgggaa gaacattttg attatatgga agacttccat aaagaaaatg gctttatgag
      781 aggaagatgg ggctgccacg gtagaggtgg ccatggaatg atgagaagat ataaaagagg
      841 ttggtaagtc taaaagagag gtgcagagat gattagaacc tggattaaag attttttaga
      901 aagacttata gcttcaaata aaaaagaatt tggaaacaaa agattagact gttgtgaaat
      961 aaaaaagatg caaataagaa agtccagact agttaaatag tctggacttt ttagccttca
     1021 aacttataac ctactccata tactgtcaag atatatttag gttcctttgg attatcttca
     1081 attttttgtc ttatattttt aatatgggta tctatggttc tatgaaagcc gtcataatca
     1141 tagccaaagg ccctatcgat tagctgttct cttgtaaaga ctatgcctgg atttgatgcc
     1201 agaataataa agattttaaa ttcattggta gtaagattta catcctgctt atttttaatt
     1261 acctggaatt tatcagtata aatttcaagg ccatttgaaa aaaccagtct ttcagccagg
     1321 attcctccat ccctataact tcttctcatt agtgccttta ccctacttac caattctcta
     1381 gggctaaagg gctttaccat ataatcatcg gcacctaagg ataggccatc tattttatcc
     1441 tcttcatggg acttggctgt aagcataata attggaacct gggatgtatt ccgaatcatg
     1501 gagcatacct tctcaccagg tatttttggg atcatcaaat ctagaataat taaatcaggc
     1561 ttatattttg caaataagtc catggcttcc tgtccatcat aggcagttat aaccttataa
     1621 ccttcctttt ccaaataggc tttaatcaca tcagatatat ttttttcatc ttctactact
     1681 aatatacaat catgggacat ggcttcacct caagattatt ttatcatatt ttttaaccgg
     1741 attagataag tttcccctcc agtatggcta aaacaaaatt tccattttct gtttttaata
     1801 cataggtctg ggtatagtta tattgtaata atatcataaa aataccaaa
//
LOCUS       DATGSI010000425         1404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_126594, whole genome shotgun sequence.
ACCESSION   DATGSI010000425 DATGSI010000000
VERSION     DATGSI010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126594"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..>1404
                     /locus_tag="VK031_06480"
     CDS             142..>1404
                     /locus_tag="VK031_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53600.1"
                     /translation="MASAYGILHNYGEPQNKLDYSLQLQGLEYRQNMYDANEVKLEQA
                     MNSLGIQRNQIVNTAAKEKLDQNVHDFLNKLNNTGELDLSSSASTKSIIGDIGKILDP
                     ETVYHIGKGREIANYSAKFEELREKEGGKYYNENNYAYGLAMAGYSDYVNGGTDAILG
                     RLEVIPYKDVHKEMNESIFKMMSKAGTKEYRDGDGMIRQIKEFTPLEARNFVMANLDD
                     KAKKQLEINSWATTQGLTSEQLEQHSENYYLSKLNVLQADIKAARRKRNSGDPTEAQH
                     AQRTLEQLERHVAELNRDYSEMRQSTQGMNQFLGTQSFIDEMINSYSSVIDDKYKKDP
                     LLSTIETANSRTGVGRERGDEDYRRTVSLIGTDLTDDESRNIIERNRQIFEQENEKAN
                     TRLDAIIATIESDPDTRDIWSNLIEDPEK"
BASE COUNT          603 a          193 c          275 g          333 t
ORIGIN      
        1 tcagatattt gagaattttg gagtagagga tacaggaaca ttcaacttaa gagaagcaga
       61 ccctgcaata ctatctgact acttaaggta tacttatcaa aatcctgtaa cacagacaac
      121 taaaaagaaa aaagaataaa tatggcaagt gcatacggaa tactgcataa ttacggagaa
      181 cctcagaata aattagacta ttctttacag ttacaaggcc tagaatatag gcaaaatatg
      241 tatgatgcta atgaagttaa attagaacaa gctatgaatt ctttggggat ccaaaggaat
      301 caaatagtta atacggctgc taaagaaaag ttagaccaaa atgtacacga tttcttgaat
      361 aagttaaaca atactggaga actcgattta tctagctcag catctactaa aagtataata
      421 ggagatatag gaaaaatact agatccagaa actgtatatc atataggaaa aggtagagag
      481 atagctaatt acagtgctaa atttgaagaa ttaagggaga aagaaggagg caagtactat
      541 aatgaaaaca attatgccta cggacttgct atggcaggat actccgacta tgtaaatgga
      601 ggtacagatg ctatattagg gagattagaa gtgattccat ataaagacgt ccataaagag
      661 atgaacgaat ctatatttaa aatgatgtct aaagcaggga ctaaagaata tagagacgga
      721 gacggaatga ttagacaaat aaaagaattt actccactag aagctagaaa ttttgttatg
      781 gctaatctag atgacaaagc aaaaaaacag ttagaaataa atagctgggc cacaacacaa
      841 ggattaacat ctgaacaact agaacaacat tctgaaaact actatttaag taaattaaat
      901 gtgttacaag cagatataaa agcagccaga agaaaaagaa actctggaga tcccacagaa
      961 gcacaacatg cacaaagaac attagaacaa ctagaaagac atgtagcaga gttaaataga
     1021 gactactcag aaatgaggca aagtactcaa ggaatgaatc aattcctagg gacgcaaagt
     1081 tttatagacg agatgattaa tagttattct tctgtgatag atgataaata taagaaagat
     1141 cctttgttat ctactataga aactgctaac agcaggactg gagtaggaag ggagagaggg
     1201 gatgaagatt atagaagaac tgtttcttta ataggaacag atctaactga tgatgaatct
     1261 agaaatatta tagaaagaaa cagacaaatt ttcgaacaag agaatgaaaa agctaataca
     1321 agattagatg caataatagc tactatagaa agtgatccag acacaagaga catatggtca
     1381 aacctaatag aagatcctga aaaa
//
LOCUS       DATGSI010000426         1299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_126727, whole genome shotgun sequence.
ACCESSION   DATGSI010000426 DATGSI010000000
VERSION     DATGSI010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126727"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VK031_06485"
     CDS             complement(<1..1276)
                     /locus_tag="VK031_06485"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HLS53601.1"
                     /translation="MAVVLQLALAIDYAIILCHRFNEERFVYDPVNAAKEALKKALVE
                     ISSSSLTTIAGLAALSFMEFGIGKDLSFVMIKAIVLSMTTVFILMPGLLVLFSGLMDK
                     TKHRSFMGNVHLLGKFSIKTRYIMPPIFAVLLIISFILSNKIPFLFSLNDIKAHRVTE
                     QQVARDRIRENFGENNMLALIVPSGDYESERKLLDNLSAYEEVSRAVGLANTEAMGNY
                     MLTDSLSPRQFAELVDLDLEVAQLLYSAYAINQEEYSKIVNGINLYEVPLIDMYQFLY
                     EEVIQGFVSLDEEQMEELEEYYTLLSGAREQMEGENYSRMILYLDLKEESEETFEFLG
                     TIRKEAERFYDKDSIFTIGESSNALDLSLTFDRDNLVISILSVLFVILILTFTFRSVG
                     LPILLISVIQASIWINFSFPYIRGDGLYFLGFL"
BASE COUNT          448 a          285 c          187 g          379 t
ORIGIN      
        1 ataaaaatcc taagaagtat aatccgtctc ccctaatata aggaaaggaa aagttgatcc
       61 aaatactagc ctgaattaca gagattaata aaataggtaa accaacagac ctaaaggtaa
      121 atgtaaggat caatattaca aacaaaacag ataatatact tataaccaaa ttatccctat
      181 caaaggttaa ggataaatct aaagcattgg aagattcacc tatagtaaat attgaatctt
      241 tatcataaaa tcgttcagcc tctttcctaa tagtacctaa aaactcaaag gtttcctcac
      301 tttcttcctt tagatccaga tacagaatca tcctgctgta gttttcccct tccatctgct
      361 ccctggcacc agataaaaga gtatagtatt cctctaactc ttccatctgc tcttcatcta
      421 ggctaacaaa accttgaata acttcctcat ataaaaactg gtacatatct atcaaaggaa
      481 cctcatataa gtttattcca ttaactattt tggaatattc ctcctgattg attgcatagg
      541 ctgaataaag aagctgggca acctccaaat ctagatctac cagttcagca aattgccttg
      601 ggcttaagct atcagttaac atataatttc ccatggcctc agtatttgcc aaacctactg
      661 ccctgcttac ctcttcatag gctgatagat tatccaaaag ttttctttct gattcataat
      721 ctccagaagg cactatcaag gccaacatat tattttctcc aaaattttcc cttatcctat
      781 ccctggctac ttgctgctct gtaaccctat gggccttaat atcatttagg ctaaacaaaa
      841 aaggaatctt gttagataag ataaaggaaa taatcaacag caccgcaaat atgggtggca
      901 taatatatct ggtcttaata gaaaacttgc ctaacaaatg aacatttccc ataaagcttc
      961 tatgtttagt tttatccata agtccactga ataagaccaa gagccctggc atcaggatga
     1021 acacagtggt catactcaaa acaatagcct taatcattac aaaggataaa tcctttccta
     1081 taccaaactc cataaaggat aaggccgcca gaccagctat agtggttaaa ctactggatg
     1141 atatttccac cagggccttc tttaaggcct cctttgctgc atttaccgga tcataaacaa
     1201 aacgttcctc attaaaccta tgacatagga ttatggcata gtctatggcc agggccaatt
     1261 gtaaaactac agccacagaa ttggatacaa aggatattt
//
LOCUS       DATGSI010000427         3331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_219770, whole genome shotgun sequence.
ACCESSION   DATGSI010000427 DATGSI010000000
VERSION     DATGSI010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219770"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VK031_06490"
     CDS             complement(<1..1233)
                     /locus_tag="VK031_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLS53602.1"
                     /translation="MNKYFDVKDKVYHITEKYPETIDVLVANGFAPLKNEKMRKLMGK
                     TISLEMACKSKMVNLHVLEEKLVEVIERTRFPEDETLLGTSRTLGGDIKIQGVLPCPI
                     RIPLLENFNRWLEDNREDLGHKVDYDLKSAHIGVDWIREEIQNKEEDQLADIFISAGF
                     DLFFDKDLFGRFKEKDVFEDLSGLESLNEDFDNQDIDLKDPERQYTVLGAVPAVFLVN
                     VNELKGREMPKSWIDLLRPEFENSVSLPLHDFDLFNAILLTLYKNYGEKGIERLGRSL
                     LKSMAPAEMVKSHIKKEDAKIPTVTVMPYLFTQMVGKDSPLKAIWPEDGAIISPIFLL
                     TKRDRRKELKPLVDFFLSKEVGQLMSENGGFPSTNPKVVNTATKGKKYMWLGWDFINS
                     NRDIGSLINECLEIFHDAK"
     gene            complement(1336..2112)
                     /locus_tag="VK031_06495"
     CDS             complement(1336..2112)
                     /locus_tag="VK031_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53603.1"
                     /translation="MGKKLFYGIIIFSIIFSLTACLNPIEPGEPIISEHTDKLEFLSE
                     TEENQLMEEYGRLLKTDRNKDEIIGFIEENIEKLTTKNASQMILSLGEYLEDNNYPVY
                     ESYILLNKYKKYVSLEAKSYINIFFSEASNPYKSGDKVRINLEELTNRAIIAEDHLDI
                     FSTGNTRDKIYNLYKSYVRGLIDHEENPYIKLEENNYRLAKETIDTYGELIEKNKGYR
                     TTNLLSDFIKILEENNYNIKSQEIKDFYQDLDLLLEEEFN"
     gene            complement(2374..2772)
                     /gene="ndk"
                     /locus_tag="VK031_06500"
     CDS             complement(2374..2772)
                     /gene="ndk"
                     /locus_tag="VK031_06500"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HLS53604.1"
                     /translation="MEKTFIMIKPDGVKRGLMGEIIKRIERKGFRINKIQLMKPSKKL
                     VSKHYAEHKEKPFFKELVSYIAGKDVLAMEVEGDSAIEVMRLMIGHRDHRQAQPGTIR
                     GDFAHQLTENIIHGSDSKEAAARELKLWFG"
     gene            complement(2891..3064)
                     /locus_tag="VK031_06505"
     CDS             complement(2891..3064)
                     /locus_tag="VK031_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53605.1"
                     /translation="MVEKALVDAGLNAQVQEWEVEFEEGVAQLEVEIMKGANKIEYKY
                     DIETGNMIDKLIK"
BASE COUNT          965 a          702 c          422 g         1242 t
ORIGIN      
        1 ctttgcatca tgaaatattt ctaaacattc attgattagg ctgcctatat ccctattaga
       61 atttataaaa tcccaaccta accacatata ctttttccct ttggtagctg tattaaccac
      121 cttaggattg gttgaaggaa aaccaccatt ttctgacatt agctgaccta cctctttaga
      181 taaaaagaag tccactaaag gctttaattc cttccttcta tcccttttag ttagcaaaaa
      241 tatggggctt ataatggctc catcttcagg ccaaattgcc tttaaaggac tatcctttcc
      301 taccatttgc gtaaacaaat agggcataac tgttactgtt ggaatcttgg catcttcttt
      361 ttttatatgg gactttacca tttctgctgg ggccatgctt tttaataagg atctacctaa
      421 cctttctatt cccttttctc catagttttt atatagggtc aagagaatgg cattaaatag
      481 atcaaagtca tgtaaaggca gggatacaga gttttcaaac tcaggcctta ataaatctat
      541 ccaacttttt ggcatctctc ttccctttaa ttcattaaca tttactaaaa atacggctgg
      601 tactgctcct aaaactgtat attgtctttc tggatccttt aagtctatgt cctgattatc
      661 aaaatcctca tttaaacttt caagaccaga aaggtcttca aatacatctt tctccttaaa
      721 tcgcccgaat aaatccttat caaaaaatag gtcaaatccg gctgatataa aaatatcagc
      781 taattgatcc tcctctttgt tttgtatttc ttctctgatc caatctaccc caatatgggc
      841 agattttaaa tcataatcaa ccttatggcc taaatcttcc ctattgtcct ccagccatct
      901 attgaagttt tccaaaaggg gaattctaat ggggcaaggt aggactcctt gaatttttat
      961 atctccacct agggttctag aggttcctag taaggtctcg tcttctggaa atctggtccg
     1021 ctcaattact tccaccaatt tttcctctag gacatgtaaa tttaccatct tgcttttaca
     1081 ggccatttcc aaagatatgg tttttcccat taattttctc atcttttcat tttttaatgg
     1141 ggcaaagcca ttggctacta atacatctat ggtttcggga tacttttcag ttatatggta
     1201 taccttatcc ttcacatcaa aatatttgtt catctgcctc ctccttctat ctctgcccaa
     1261 acatttttac acattttaat ctattatata ttaaacataa aaaaaggtcg gtaacaaaag
     1321 ttacctaccc ttactttagt taaattcttc ctctaacaac aggtctaaat cctgataaaa
     1381 atctttaatt tcttggctct ttatattgta attattctcc tctaaaatct ttataaagtc
     1441 agacaaaaga ttagtagttc tgtacccctt atttttctct atcagttccc cataagtgtc
     1501 aatggtttct ttggctaacc tgtaattatt ttcctccagt ttaatataag gattttcttc
     1561 atggtctatt agccctctta cataagattt atacaggtta tatatcttat cccttgtatt
     1621 gccagttgag aatatatcta aatgatcctc tgctataatg gctctgtttg ttaactcttc
     1681 taaatttatt cttaccttat ctccagattt gtagggatta ctagcctcag aaaagaatat
     1741 atttatataa gattttgcct ctaaagatac atatttttta tatttgttaa gtaaaatata
     1801 agattcatag actggataat tattgtcttc caaatattct cccaggctca atatcatctg
     1861 gctggcattt ttggtggtta acttttcaat attttcctct ataaatccta taatctcatc
     1921 cttatttcta tcagtcttaa gtagccttcc atattcctcc atcaattgat tctcttcagt
     1981 ttctgataaa aactccagct tatcggtatg ttcactaatt ataggttccc ctggttctat
     2041 aggatttaaa caggctgtta gggaaaaaat aatagagaaa ataataattc cataaaagag
     2101 tttctttccc ataggacctc attcccttct tgacttcctt atattttata tataccacaa
     2161 gaagaagaaa tatatggtta caatattgtt acaaagttaa caacttgtct aggcctgtaa
     2221 tgaattgtct tttaggtcct acgataaata taaaaaaccc ctgtaaatta cagggatttt
     2281 aaatctatat cctaaagtga caaatatatc attctatctt tacaagccta aattaagaaa
     2341 ttaacacaat ttattcatat gtttagttaa gttttagcca aaccagagct ttaactccct
     2401 tgctgctgcc tccttagaat cagaaccatg aattatattt tcagtaagtt gatgggcaaa
     2461 gtctccccta atagttccag gttgggcctg tctatgatcc ctatgcccta tcattagcct
     2521 cattacttct atggcagaat caccttccac ttccatggct aaaacatctt ttcctgctat
     2581 gtagcttact agttccttaa aaaagggctt ttctttatgt tctgcataat gcttacttac
     2641 cagtttctta ctaggtttca ttagctggat tttattgatt ctaaagccct ttctttcaat
     2701 tctttttatt atctctccca ttagccctct ttttacccca tcaggcttta tcattataaa
     2761 agtcttttcc atcctatctc ccttcctata tctgattatt aattattaat acccattttc
     2821 tctcctttta taaagtctgg ataatttttc atttaaaagc ggcaggtgtt aacctaccgc
     2881 ctttaagagc ttactttatt aacttatcaa tcatattgcc tgtttctata tcatatttat
     2941 attctatctt atttgcacct ttcattatct caacttctaa ttgggctact ccttcttcaa
     3001 actctacctc ccactcctga acttgtgcat ttaatcctgc atcaactaag gctttctcta
     3061 ccaaggctgg aaccttttct aagtgagatc tgcttatttg tccaaaataa taatatcaaa
     3121 gattaggagg tattgcaaat attttaacaa tccttgtgct aagttaaact gataaccttg
     3181 ttgtatcata tacctttcta ttttcctctg ctggtagaat tataagaata aatatggcca
     3241 ctaggcctat acaggataga ataaccacaa ccatcctata tcccataaga tctcctaaaa
     3301 accctgccag catttgaaaa cctattactg a
//
LOCUS       DATGSI010000428         1358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_219779, whole genome shotgun sequence.
ACCESSION   DATGSI010000428 DATGSI010000000
VERSION     DATGSI010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219779"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1153)
                     /locus_tag="VK031_06510"
     CDS             complement(32..1153)
                     /locus_tag="VK031_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLS53606.1"
                     /translation="MFNRIFRIMFAVIGAILGISLIKLLLSVVSFTIDSSIFLIAINV
                     LVALIFALLFYFLSGLIFKRLESSFIYIEEYLENMPPLSVAVGIAGVIVGLILAFLVT
                     RPLTSLSLPIVGNSIFVLLSIIIYIGFGLLSWRIASKYPEKYPQIFNLFKEEKKKKDS
                     PKEEKNEEITSKESKPSTTKIIDTSVLIDGRILDIIKISFLEGKLIIPEFVLEELQHI
                     ADSPDDLKRERGRRGLDVVGKIQEDEDVDLEISPVDYKDIKEVDSKLLKYAKDINAKI
                     FTNDYNLNKVADLQGITVLNINDLANALKTKVLPGESLEIEVIKEGKSRNQGVGYLED
                     GTMVVIEDGKNLIGQTINSTVTSVLQTSAGKMIFVRAKG"
     gene            complement(1167..>1358)
                     /locus_tag="VK031_06515"
     CDS             complement(1167..>1358)
                     /locus_tag="VK031_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HLS53607.1"
                     /translation="TGDIFKIAKMVRDLVALDEEKGLSTTEKKLLNRARRIMASELVM
                     AGAIEREEAEKMIDESIGL"
BASE COUNT          407 a          267 c          187 g          497 t
ORIGIN      
        1 tactgctgat actttcattt cacctatcat attatccctt tgctcttaca aaaatcatct
       61 ttccagctga tgtttgtaaa acacttgtta ctgttgaatt tatggtctgt cctataaggt
      121 ttttgccatc ttctatgaca accatggttc catcttcaag atatcctact ccttgatttc
      181 ttgacttacc ttccttgata acctcaattt ctaggctttc tccaggtagc accttggttt
      241 ttagggcatt ggccaagtca tttatattaa gtactgtgat tccttgtaag tctgcaacct
      301 tgtttaggtt gtagtcattg gtaaaaatct tggcattaat gtctttggca tattttaaaa
      361 gcttggaatc tacttctttt atgtccttat agtcaacagg gcttatttct aagtctacgt
      421 cttcatcttc ttggattttt cctacaacat caaggcccct tcttcccctt tctcttttaa
      481 gatcatcagg gctgtcggca atgtgttgta attcttctaa gacaaactct ggaattataa
      541 gttttccttc taaaaaactt attttaatta tatcaagaat cctaccatct atcaaaacag
      601 atgtatcgat aatctttgtg gtagaaggct tactttcctt gcttgttatt tcttcatttt
      661 tttcttcttt aggactgtct tttttctttt tttcttcttt gaaaagatta aagatttgtg
      721 ggtatttttc aggatacttt gaggcaatcc tccaagaaag aagaccaaaa ccaatataaa
      781 taataataga taatagcaca aaaattgaat taccaacaat tggtaatgat aatgaggtta
      841 agggtcttgt tacaagaaag gccagtatta atccaacgat tacacccgca attcctacag
      901 ccacgctcaa aggcggcata ttttcaaggt attcttctat gtagataaaa cttgattcca
      961 accttttaaa aataagtcca gataaaaaat aaaataataa ggcaaaaatc aaagctacta
     1021 agacattaat tgcaattaaa aaaatagaac tatctattgt aaagcttaca acagatagta
     1081 gcaattttat taaagaaatg cctagaatag ctccaattac tgcaaacata attcgaaata
     1141 ttctattaaa catattctct cctatattat aggcctatag attcatcgat catcttttcg
     1201 gcttcttctc tttcgatagc gccggccata accaattcac tggccataat ccttctagcc
     1261 ctatttaaaa gcttcttttc agtagttgaa aggccttttt cttcatcaag ggctactaag
     1321 tctcttacca tttttgcaat cttaaatata tcacctgt
//
LOCUS       DATGSI010000429         1004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_501865, whole genome shotgun sequence.
ACCESSION   DATGSI010000429 DATGSI010000000
VERSION     DATGSI010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501865"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..>1004
                     /gene="galE"
                     /locus_tag="VK031_06520"
     CDS             98..>1004
                     /gene="galE"
                     /locus_tag="VK031_06520"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HLS53608.1"
                     /translation="MTILVTGGAGYIGSHTCVELLEEGHDIIIVDNFSNSQPQVIEKI
                     KEITGKDFKHFQIDLLDREALEKVFKENKIEAAIHFAGYKAVGESVEEPLKYYHNNIL
                     STINLCQIMDKYRVKKLVFSSSATVYGDENKSPLTEELPRKGTNPYGYTKIMIEQILN
                     DLYISDKDWSISILRYFNPIGAHESGKIGERPTGIPNNLMPYITQVAAGRLDKLKVFG
                     KDYNTHDGTGVRDYIHVVDLAKGHIKALDKINNSKPNIYTYNLGTGVGYSVLDLVKTF
                     EKVTGQKVPYEIVERRPGDVATCYAD"
BASE COUNT          365 a          148 c          220 g          271 t
ORIGIN      
        1 agtagttaca aatatggctt tgtgatataa tatgtatata attaagttag aaacttttgg
       61 gctaggccca gtttacaatt aaatcaggag gataaaaatg accatactag ttacaggagg
      121 ggcaggctat ataggcagtc atacttgtgt ggaactttta gaagaaggac atgatatcat
      181 aatagttgac aacttttcca atagccagcc acaggtgata gaaaaaatta aggaaattac
      241 agggaaggat tttaagcact ttcaaataga cctattagat agggaagctt tggaaaaggt
      301 ttttaaagag aataagatag aagcagcaat acattttgcc ggttataagg ctgtaggtga
      361 atctgtggaa gaacctttaa aatattatca taataacata ttaagcacca taaacctatg
      421 ccagattatg gacaagtata gggtaaaaaa attggtcttt agctcttctg ccacagtcta
      481 tggagatgaa aataaatcac cacttacaga agaactacca aggaagggta ctaaccctta
      541 cgggtacacc aaaataatga tagaacaaat cttaaatgat ttatatatat ctgacaagga
      601 ttggagcatt tctattttaa ggtacttcaa cccaattggt gcccatgaaa gtggcaaaat
      661 aggtgaaagg cctactggga ttccaaataa cctaatgccc tatattacgc aagtggcggc
      721 aggtaggcta gataagctta aggtatttgg aaaggactac aatacccatg atgggacagg
      781 tgttagagac tatattcatg tggtagattt ggccaagggc catataaagg cgttggataa
      841 gataaataat tctaagccta atatatatac ctataatttg ggaactggag taggctatag
      901 tgtcttagat ttagtaaaga cctttgaaaa ggtgactggt caaaaagtgc cctatgaaat
      961 agtagaaaga aggccaggag atgtggccac ctgctatgca gatc
//
LOCUS       DATGSI010000430         1539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_359687, whole genome shotgun sequence.
ACCESSION   DATGSI010000430 DATGSI010000000
VERSION     DATGSI010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359687"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VK031_06525"
     CDS             <1..357
                     /locus_tag="VK031_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HLS53609.1"
                     /translation="KALAEALKEKGFRLVSDGTDNHLILIDLRNKGLTGKEAEEILED
                     VGIATNKNTIPFDPESPFITSGIRIGTPAVTTRGMKEKEMEEIADIISLVLHKEISKQ
                     EALDRVNQLCHKFPLY"
     gene            421..>1539
                     /locus_tag="VK031_06530"
     CDS             421..>1539
                     /locus_tag="VK031_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HLS53610.1"
                     /translation="MELTNINISKNIIEDVEPNSIGKELGIEPGDILLSINGNPVRDI
                     IDYKYLISDEYIELEIEKDEGEIWLYEIEKEYDEDLGIIFTNPLIDRAKSCSNKCIFC
                     FIDQLPPNMRESLYFKDDDSRLSFLQGNFITLTNMSDEEIDRIIKYRLSPINISVHTT
                     NPKLRVKMLRNRKAGNILEILKKFHQAQLEINCQIVLVPGFNDGEELDKTLTDLAGLH
                     PSIKSVAVVPVGITKYRERLTHLTPYNSDMAKKLLEQVLNKQEYFLKELGTRFVFPSD
                     EFFALSSKDLPKYEEYENFPQVENGVGLLKLFKSQVMEELEKIDEPIKNKGEYIIATG
                     TLAYEFMKDIAKKVNQKFQGLKLTVVPIINQYFGEKITV"
BASE COUNT          597 a          205 c          304 g          433 t
ORIGIN      
        1 aaggccctgg ctgaagctct gaaagaaaag ggttttaggt tagtatctga tggtacggat
       61 aatcatttaa tcttaataga cttgagaaac aagggattaa caggaaaaga agcagaggaa
      121 attctagagg atgtaggaat agctacaaat aaaaatacta ttccctttga tcctgaaagt
      181 ccatttataa ccagcggaat caggatagga acaccagctg taactacaag aggaatgaaa
      241 gaaaaggaaa tggaagagat tgctgatatt atatccttag tacttcataa ggagatttcc
      301 aaacaggaag ctttagatag ggtcaaccaa ctatgtcata agtttccttt atactaattg
      361 tacctaggcc tatctagtca taggcttagg tagtgtgata atttaacaag ggtggtaaaa
      421 atggaattaa caaatataaa tataagtaaa aatataatag aggatgtaga accaaacagt
      481 ataggcaagg aattaggaat tgagcctgga gatatactat tatctattaa tgggaatcca
      541 gtaagggaca taatagatta taaatacttg atctcagatg aatatataga attagagatt
      601 gaaaaggatg aaggtgaaat ttggctgtat gaaattgaaa aggaatatga tgaagactta
      661 ggtataatat ttaccaatcc attaatagat agggcaaaat cctgcagcaa caagtgcatc
      721 ttctgcttca tagaccaatt accacctaat atgagagagt ccttatattt taaggatgat
      781 gattcaaggt tatcttttct acaaggaaat tttattacct taactaatat gagtgatgaa
      841 gaaatagaca ggattataaa atataggtta agcccaatta acatatcagt ccatacaact
      901 aatccaaaac tgagagtaaa gatgttgaga aatagaaaag ctgggaatat attggaaata
      961 ttaaagaaat tccatcaggc ccaattagaa ataaattgtc aaatagtttt ggttcctgga
     1021 tttaatgatg gagaagaatt agataagacc ttaactgatc tggcaggcct acacccttct
     1081 attaaatctg tggcagtggt cccagtggga attacaaagt acagggaaag attgacacac
     1141 cttactcctt ataactcaga tatggctaag aaattattag aacaggtact aaataagcaa
     1201 gagtatttcc taaaagaatt aggaacaagg tttgtttttc cttcagatga attttttgca
     1261 ctttcatcta aagacctgcc taaatatgaa gaatacgaga attttcccca agtggaaaat
     1321 ggagttggat tactgaaatt gtttaaatcc caggtgatgg aggaattgga aaaaatcgat
     1381 gagcctatta aaaataaagg tgaatatatt attgcaacag ggaccttggc atatgagttt
     1441 atgaaagata ttgctaaaaa ggttaatcaa aagtttcaag gtttaaaatt aactgtagtc
     1501 cccataatta accaatattt tggagaaaaa ataaccgta
//
LOCUS       DATGSI010000431         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_261704, whole genome shotgun sequence.
ACCESSION   DATGSI010000431 DATGSI010000000
VERSION     DATGSI010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261704"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..158
                     /locus_tag="VK031_06535"
     CDS             9..158
                     /locus_tag="VK031_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HLS53611.1"
                     /translation="MAIDGGENAAILAAQILSLKYPELKESLKIYKEELAKDVEKMNE
                     TLRTT"
     gene            171..>1507
                     /gene="purF"
                     /locus_tag="VK031_06540"
     CDS             171..>1507
                     /gene="purF"
                     /locus_tag="VK031_06540"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HLS53612.1"
                     /translation="MSGIVAIFSKEREHVSQIMYYGLYGLQHRGQISTGIAVNNNGFV
                     DYFKDMGLVHEVFPKEVMDRLRGNIAVGHVRYAYEDEAKSIRNIEPLVVGYRRGALAL
                     AHDGNIVNFAQLRDELEDKGTIFQSDLDAEVIANLIARYHKNNVEEAVIRATKDLIGS
                     YGLVIMTNDKLIGARDPFGIKPLSLGRTKDSYVLASETCAFDTMGAEFIRDIRPGEII
                     VIDDYGTRSIYEGLRNRRLCLFELIYFARPDSRVDGKSIYLARIEAGRILAREAPAKA
                     DIVIGAPDSGIVAAIGYAEESKIPYAEGIIKNRYVGRTFIHPTEELREEGVRIKLNVL
                     RENIENKSIVLVDDSIIRGTTIRRTVEMLKQAGAKKVHVRIAAPPVKYPCHLGMDTRD
                     RKHLIAANLDLENLRKKTGADSLAYLSLEGSLEAVDGSSGFCRGCFTGRYPVE"
BASE COUNT          488 a          228 c          396 g          395 t
ORIGIN      
        1 tggcaactgt ggccattgat gggggagaga atgcagccat attggcagcc cagattcttt
       61 cccttaagta tcctgaatta aaggaaagtt taaagatata taaagaagag ttggccaaag
      121 acgtggagaa gatgaatgaa actttaagaa ctacctaagg gggccctgtt ttgagtggaa
      181 tagttgctat tttcagtaaa gaaagagaac atgtttccca aatcatgtat tatggcctct
      241 atggcctcca acataggggg cagataagta ctggcattgc agtcaataac aatgggtttg
      301 tggattactt taaggatatg ggcctggttc atgaagtttt tcctaaagag gtaatggata
      361 ggctaagggg aaatatagct gtaggccatg taaggtatgc ctacgaagat gaggctaaaa
      421 gtataagaaa tatagaaccc ttggtggtag ggtatagaag gggggccttg gccctagccc
      481 atgatggtaa catagtaaac ttcgcccagc taagggatga attggaggat aaggggacca
      541 tctttcaatc agatttggat gcagaagtta tagccaacct aatagccaga tatcataaga
      601 ataacgtgga agaggcagta attagggcca ccaaggacct tataggttca tacggattag
      661 ttattatgac caatgataaa ttaattgggg ctagagatcc ctttggcata aaacccctat
      721 ctctaggaag aaccaaggat tcttatgttt tagcatcaga gacttgtgcc tttgatacta
      781 tgggagctga atttattaga gatattaggc cgggagaaat aattgtaata gatgattatg
      841 gcactagatc tatctatgag gggttgagaa acagaagact ttgccttttt gaattaatct
      901 attttgcaag accggacagc agggtagatg gaaaaagtat ttatttggca aggatagagg
      961 ctggtaggat tttggccagg gaggctcctg caaaggcaga tatagtaata ggggcaccag
     1021 attctggcat agtagcggcc ataggttatg ctgaagaatc aaaaattcct tatgcagaag
     1081 gaattattaa aaaccgttat gtaggaagaa cttttatcca ccctaccgag gaattaaggg
     1141 aagagggggt taggataaaa ctaaatgtct tacgagaaaa tatagaaaac aaaagtattg
     1201 ttttggtaga cgattcaatt ataaggggaa caactattag aaggactgtg gagatgttga
     1261 aacaggcagg ggccaaaaag gttcatgtaa gaattgcagc accccctgtc aagtatccct
     1321 gtcatctggg catggataca cgtgatagga aacacctaat agctgccaat ttagatttag
     1381 agaacttaag gaagaagaca ggggcagatt ctttagccta tctatccttg gagggttcgt
     1441 tggaggctgt agatggaagt agtggttttt gccgagggtg ctttactggg agatacccag
     1501 tggaaag
//
LOCUS       DATGSI010000432         1005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_28257, whole genome shotgun sequence.
ACCESSION   DATGSI010000432 DATGSI010000000
VERSION     DATGSI010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28257"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VK031_06545"
     CDS             complement(<1..707)
                     /locus_tag="VK031_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HLS53613.1"
                     /translation="MGDKRTDIDIIYGINDSPELTRAIPLAFQHILAMFAGNITIPLL
                     LASLLNLPPSETAFLIQCSLFVAGIATFIQIVKFKGIGSGLPIVMGTSNAFVSTVLAI
                     GKDFGIEGVLGASFIGGIFEVFLGKNLNRLKKIFTPLVSGIVVLTIGITLIPVGIRQA
                     AGGSGNMGDLKSILIASIVLLAIIVFNQSSNKFFRSSSILLGIVVGYFVSYFFGMLDI
                     GPIFEASWFSIPKPFVY"
     gene            complement(759..>1005)
                     /locus_tag="VK031_06550"
     CDS             complement(759..>1005)
                     /locus_tag="VK031_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53614.1"
                     /translation="AFAAGVLTLTLPTLSALGLPSEAMGFVAISVGLGTQVSPVQINV
                     AAMAKGYEMDIMEIIKHNLRYVLGTLVLVMIIAVIVV"
BASE COUNT          336 a          211 c          157 g          301 t
ORIGIN      
        1 ttataaacaa aaggtttagg gatagaaaac caactggctt caaagatagg ccctatatct
       61 aacattccaa agaagtagga tacaaaatac ccaaccacta tgcctaataa tatggaagat
      121 gatctaaaga atttattgct actttgattg aatactatta tggctagaag cactatagaa
      181 gctattaaaa tgctttttag atcccccata tttccactgc caccggcagc ctgcctgatt
      241 cctacaggga ttagggttat accaatagtt aaaactacta ttccagatac tagtggtgta
      301 aatatctttt tcaacctgtt aaggtttttt cctaaaaaca cttcaaatat accaccaata
      361 aagctggccc ctaatactcc ttctattcca aagtcctttc ctatggccaa aacagtggaa
      421 acaaaggcat tgctagttcc cataactata ggcaggccag accctatgcc cttaaacttt
      481 actatttgta taaaggtggc aatacctgca acaaacaggg aacattggat taaaaatgca
      541 gtttcactag gaggcaagtt taataaactt gccaataaaa gtggtattgt tatattacct
      601 gcaaacatgg ccagtatatg ttgaaaggct aaaggtatgg cccttgttag ctctggacta
      661 tcattaattc cataaattat atctatatct gtccttttat ctcccataat ctcactccgt
      721 tgtgtaacat taatgaggac tcattaatat ttaaattttt atacaacaat tactgcaatt
      781 atcataacta aaactagggt tcctaaaaca taccttaagt tatgtttaat tatctccata
      841 atatccattt catatccctt agccatagca gccacattta tctgaacagg gcttacctga
      901 gttcctaatc caactgatat ggccacaaat cccatagctt cggaaggtaa ccccaaggct
      961 gataaggttg gtagggtcaa ggtcaaaact cctgctgcaa aggct
//
LOCUS       DATGSI010000433        18868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_408086, whole genome shotgun sequence.
ACCESSION   DATGSI010000433 DATGSI010000000
VERSION     DATGSI010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 18868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18868
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408086"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(378..515)
                     /locus_tag="VK031_06555"
     tRNA            complement(378..515)
                     /locus_tag="VK031_06555"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(557..763)
                     /locus_tag="VK031_06560"
     CDS             complement(557..763)
                     /locus_tag="VK031_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53615.1"
                     /translation="MADVKTIKGWVNIIYEVRCPHCFVGYSTLFNPDRFAQFTEVEDG
                     EDTEFDARCRECTKDFKVAGFEPA"
     gene            complement(1187..1432)
                     /locus_tag="VK031_06565"
     CDS             complement(1187..1432)
                     /locus_tag="VK031_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53616.1"
                     /translation="MKTVIKHLENIPLDSSWTIEVVRNNLIILSYEDMILRLFTKEGV
                     ANGATWGAIRHLLRNGLATGVDLRIGEDKITNIENYV"
     gene            complement(1674..2219)
                     /locus_tag="VK031_06570"
     CDS             complement(1674..2219)
                     /locus_tag="VK031_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53617.1"
                     /translation="MKSLQVLTDMPESTQWSVDSISTSIQMSCVEKGFHFILDINTRQ
                     ANLMSFDWGSIKKEILANDRIVSPKVLIQNPDGTRSEKLPDLKEYLRYNDVRHVVKNM
                     DPNMEWAVESINWDAVSLTARDSSHVYHLYIRQDTYLRAPITWEALKSGIMDGSITKS
                     ILKVDSREDIKDLKTFLKSIK"
     gene            complement(2236..2478)
                     /locus_tag="VK031_06575"
     CDS             complement(2236..2478)
                     /locus_tag="VK031_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53618.1"
                     /translation="MLVASHQIEVLELNYWKSDVLHIERNDYAYNIYYTYRITYDNKL
                     DLLVDDIEVFYEGDELEVSDYVMERLKEIAVELFFI"
     gene            2700..2876
                     /locus_tag="VK031_06580"
     CDS             2700..2876
                     /locus_tag="VK031_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53619.1"
                     /translation="MRAIGLNARLVFDCSALITGSRIKGSRIKGSKVTRIQDKRIQDK
                     RIQDKRIKGNQDPG"
     gene            complement(3026..3289)
                     /locus_tag="VK031_06585"
     CDS             complement(3026..3289)
                     /locus_tag="VK031_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53620.1"
                     /translation="MKLYEIIEKVIAGGWLNYNDVHPYRESIKKYCTDYFCAGPIIFL
                     VGSATNTGVSGYIVPEGDLMEKVNEITTCEDEQEIVLFNSFLL"
     gene            complement(3300..3644)
                     /locus_tag="VK031_06590"
     CDS             complement(3300..3644)
                     /locus_tag="VK031_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53621.1"
                     /translation="MKFKKDFLATCSHPELFNNLLNSGGVPWADLRRWPDDYISAESG
                     IPGLTYYNETEKIYNQNSGRIHRYFDYLNNEGLEIVNPYCPINEEVSYKNWAVWVAWE
                     SMMSDIIHFLES"
     gene            complement(3673..3906)
                     /locus_tag="VK031_06595"
     CDS             complement(3673..3906)
                     /locus_tag="VK031_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53622.1"
                     /translation="MKLLIDGSLGVYIPQKFAEDHADILINSSELIDELEILKDGPDQ
                     DEYWFAWDHILDNAECADNGYLYQDMDLWVCSE"
     gene            complement(4411..4878)
                     /locus_tag="VK031_06600"
     CDS             complement(4411..4878)
                     /locus_tag="VK031_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53623.1"
                     /translation="MIKQIRVVESQVRIVSYGRWMVKVVIEYDNDLMYLKHYTNSSTL
                     VDKYSDAELPVDADKYIFYSFISRFEDELSGWIGSSLPIKIWEIQYIIDTHYHVQDRI
                     EQLESSGYKIDHKFMLSGGVGQLKKVGKEYRMQIGYGKGRYNLAPAVILKNDS"
     gene            complement(4897..5238)
                     /locus_tag="VK031_06605"
     CDS             complement(4897..5238)
                     /locus_tag="VK031_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53624.1"
                     /translation="MKKDIKNWIEGLDFDPDKAIDASSRGCISQRFEVQFPGCKGIFQ
                     TSLPRDPLKPAYDFVVLFHKLPLDTTVDVELHTWDNEEGFGYPIIFSDEPMDSLEKGY
                     QLARHLKVAFN"
     gene            complement(5235..5639)
                     /locus_tag="VK031_06610"
     CDS             complement(5235..5639)
                     /locus_tag="VK031_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53625.1"
                     /translation="MEKGVEIFGDIKIVPLYLLLNKSNNVNMKIRKVKLSNVEKRVMS
                     RIHQIVREEDYNTALNIILDLCTPIKTMYPTQLKWVIPIFWREEPYFIASEHDETFDN
                     HELIKGMKNLLSYFGEGYNEFSDQFLEKIFEI"
     gene            complement(6434..7072)
                     /locus_tag="VK031_06615"
     CDS             complement(6434..7072)
                     /locus_tag="VK031_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53626.1"
                     /translation="MQTKSKKYLEMKPEISYSPLHKRVFRTEEERTEDLDLIIELKST
                     NHTWLEITDKLNARNRDYVVTRREIRSQYHAFLKANQDMVDQELERLAILEELDEVYD
                     LALNAFRGTLEPDEQLQTHYRDIGKGLGQEVLKRIKTTRRGKGSNGFLNTMVKVIETR
                     AKILDLYSDLTVKVEDLPEVEPPASLQSPMSSEEQLRDMFNRLEADNRDNTY"
     gene            complement(7375..7956)
                     /locus_tag="VK031_06620"
     CDS             complement(7375..7956)
                     /locus_tag="VK031_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53627.1"
                     /translation="MNNDRHIAYQFKEGKFLYRTPEQVEKAIDNKESWYHKGEYVLVN
                     SRGYIFDSSSIKVFKKANKALKFSNDTVYITVEGKKLTKGVSVRSYSGELSARYREGQ
                     FLRVVRKGYYKRYLWGWGVDNKEEIMEAVEHYQDYPEVIYMGVDDNQLKYTPLEELAK
                     WLLDNKRGVFQDADIPKSSKQAIMRDLGYSSEE"
     gene            complement(7949..8377)
                     /locus_tag="VK031_06625"
     CDS             complement(7949..8377)
                     /locus_tag="VK031_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53628.1"
                     /translation="MERISKEESGLDIHTVNLKEENNQAGKEYITDYTEEVPLGMGSI
                     KVRSLYYQVINDQPVFRTPNEVDKAKKVDDSWYDKGEYCIIGFINDQPVKYEGSGVEC
                     PHIIDKILATRLASYKHISPKLDGRRIRTLTEFYNKIENE"
     gene            complement(8364..8627)
                     /locus_tag="VK031_06630"
     CDS             complement(8364..8627)
                     /locus_tag="VK031_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53629.1"
                     /translation="MVDKTVNRIIHYYREDIDHMMDNDLKESRNNKIPTISLLARKYY
                     PAVSEEQLEYIRNEVLERSREIVNLKLGIKRSETLKRQFIGAH"
     gene            complement(8637..9056)
                     /locus_tag="VK031_06635"
     CDS             complement(8637..9056)
                     /locus_tag="VK031_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53630.1"
                     /translation="MVELLKRYFHVEVIGSYLFFEKGLLDEVNDIDILVNRDYIKQVV
                     SFLEDNGYKRSNHSEHTYNHDKSSRVRRNKHYQLVKGGKTTVHLIQSDTDRVTDLPDI
                     IKYKYERGTNTDLRQIIEICRNRLIDRGSKLDIYTPC"
     gene            complement(9163..9513)
                     /locus_tag="VK031_06640"
     CDS             complement(9163..9513)
                     /locus_tag="VK031_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53631.1"
                     /translation="MHERVATLLSDPYVGRELHKLIEEVIRSEYKSREPIPYKSKFTG
                     TSGLWTYTDCKVITDRHQTVATVFNEDDGYLMARSKDMFYLLLQIYKSDPDDIDFTEL
                     KDRINTLLTGVLGQ"
     gene            complement(9482..9676)
                     /locus_tag="VK031_06645"
     CDS             complement(9482..9676)
                     /locus_tag="VK031_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53632.1"
                     /translation="MAKWEIKTGEGDSIYHIVDTDSGELVAKIYANHLQTALDRANLI
                     AKAPELEKKINARESSYTII"
     gene            complement(9657..9914)
                     /locus_tag="VK031_06650"
     CDS             complement(9657..9914)
                     /locus_tag="VK031_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53633.1"
                     /translation="MRKYIYKEPKHHVGDKVWYSSQKGRDQEGTVRVVTTEWEKGEDG
                     AWYSKHKYSISEFGATYRRHVYTEAIFHNYTQEEIDGKMGD"
     gene            complement(9895..10227)
                     /locus_tag="VK031_06655"
     CDS             complement(9895..10227)
                     /locus_tag="VK031_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53634.1"
                     /translation="MIQLWQLAIYLPYGLKGIDDEGNLYVLKGIQIKEFKDTVLDCIW
                     QDADGKDHKLAITDIKPLLNPVPEGLQLESEPGSYPMETILGYGVFHLDVFDLLTKNL
                     AENAEVYL"
     gene            complement(10224..10385)
                     /locus_tag="VK031_06660"
     CDS             complement(10224..10385)
                     /locus_tag="VK031_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53635.1"
                     /translation="MYALVRDIVSALGVTEYRARKTAEKLIREGYIKVESYNEPSWIT
                     LHGKGYKVI"
     gene            complement(10483..10689)
                     /locus_tag="VK031_06665"
     CDS             complement(10483..10689)
                     /locus_tag="VK031_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53636.1"
                     /translation="MESEVKKAVYQVVDGVERIGYGELSIVYSRVRRILGINKKKEII
                     DKYIIEYYADKYRGLQEDYRATQA"
     gene            complement(10738..11055)
                     /locus_tag="VK031_06670"
     CDS             complement(10738..11055)
                     /locus_tag="VK031_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53637.1"
                     /translation="MSNVVMGLVLGVVVSSFIYNYPAVKQFKEEYKELDNKVFLYSSG
                     ALHSHDLNVIMYRDKSIDVKGREIRSGIRYKLDPYTYYWYMKYRYYLSRQIYKMVDKY
                     DPR"
     gene            complement(11048..11215)
                     /locus_tag="VK031_06675"
     CDS             complement(11048..11215)
                     /locus_tag="VK031_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53638.1"
                     /translation="MVRKIIYILNEMIRDYNKCKEEEGVEEDNLEELDYKHREVINDV
                     NKILRDGKGNE"
     gene            complement(11205..11498)
                     /locus_tag="VK031_06680"
     CDS             complement(11205..11498)
                     /locus_tag="VK031_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53639.1"
                     /translation="MVKYKRIYLESLGLDRHDFIPCEVTGSEAVDIHHICTREDRIEN
                     LMAVTRKVHIKYGDKKRYMVKLLTRHRQYLDIHNVKYDEEWFEEKLNRYKHGA"
     gene            complement(11491..12759)
                     /locus_tag="VK031_06685"
     CDS             complement(11491..12759)
                     /locus_tag="VK031_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLS53640.1"
                     /translation="MKLTPRQLEVKEALLDYLDKPEGYQLLYGFAGTGKTVTISYTLK
                     EYVYSRGGKIAMTAPVNKAVKVLRDTIRVPGAEYATIHSLLGLMPKVNDRTGEQEFVQ
                     DRTKGNNIRNYDAVVVDESSMLNEELFTYLMESKVPIIFVGDYKQLRPVGDENKVAAP
                     FRRSNREQYNIVPHGLTDIIRQDTASGIIPLSAYIRKGLYTPSVVNRFIKEYNDLGRI
                     EMPEILDMYKQEDYRNNPDYCKVIAYTNRQVDEINTDIRNYIYDSPNQKIVPGERLVV
                     NKPIVNGPVVLYTTNDELEVKSYSIAELPYKDKLFKVYRCDVGYGNEIDILHEDSEDQ
                     YIATVHAYKQMALTTKQGRYWRYMYSFMERFADVGYSYAITCHKSQGSTYENVVVCHR
                     DFNICRDNEDRQSLWYVGVTRASKKVYFYG"
     gene            complement(12756..13139)
                     /locus_tag="VK031_06690"
     CDS             complement(12756..13139)
                     /locus_tag="VK031_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HLS53641.1"
                     /translation="MSKIFLEVDGSYYYKRTRLGVGIVIREGETIKKFAGVWDTDSTS
                     IKAEYLSLINGLALILGTHACRKLHVTMDCQPVVRQLQGHYNVADHLKPYYSRAIQLL
                     SLFEYEIEHKKRQYLKESDKLSKKL"
     gene            complement(13120..13311)
                     /locus_tag="VK031_06695"
     CDS             complement(13120..13311)
                     /locus_tag="VK031_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53642.1"
                     /translation="MSSINIPGLEALTIEEFAAKYSMLVTEVNKLIDKGKIEVLADQQ
                     RVLYVVMTPKTKEYVKNLP"
     gene            complement(13308..13796)
                     /locus_tag="VK031_06700"
     CDS             complement(13308..13796)
                     /locus_tag="VK031_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53643.1"
                     /translation="MIMELFMTRINSKRKGNRNERRACRFMEEWTGKEFIRVANSGGI
                     HNEAIWLAGDIVPVDIEFMKEFDTVIETKHLKYLGNLEGTRSNSIIPNIMKQAEADMV
                     RVGASNCLGLLRQNRITDYVVTVPVNDRNKEYPWFLRLDNYYLFWGSTILKENISYKD
                     II"
     gene            complement(13771..15669)
                     /locus_tag="VK031_06705"
     CDS             complement(13771..15669)
                     /locus_tag="VK031_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLS53644.1"
                     /translation="MRNVDIKQVDIKGFGSILDLTYNLYRPGLNIIVGENGVGKTTLF
                     NAIFWCLFGRTTKNVVNSDIPTLKEYRGKGFKGTYVSIDMEIDGHPYKVVRTYRNKHS
                     FPYLVDPSGLTMIDKVKEIDLLNEVKLADSQRILESKLGMDATTFQNSVFFGQNTDRL
                     MDSPDGDKRKIFEDILAHIVSDLDTIKNKVQEDRREQTNENNKVINKINREEGRLTAL
                     QDQYEHLGKDEEHAREILNAKLAEINKRIDSEVDMGRRAKKSNEEANKEYARVVEVEA
                     KIRQRIKMVESKYNFREIEERYINYSSEHQSLQRSIQQTRDTVTKITTINEGDTCTVC
                     GSIVTSDTIERVTKEAEESAKEAERQVQEMRKEMYQVKQNMEAAKKQLDIRNTLETLK
                     EKHRLAKDQLESIRNTVETNNYTLKTVKERIQYLNERYAELEDEYEETIKKIEHIKDE
                     IDKQEAIVTELKVESKKYEDKIKYLDWWSRVGLGSGGVTTYLYESILVAFNKALTRYS
                     EVLGMAVRLTIDKTKKSRPFLLECYDKGVYKNYKSLSGGEQQRVNIGILFALYEVISS
                     KTVTMNILLLDEVFEGLDEVGTSMVFEIIKMYQDKSIYVITHNKDKVTDGVEFIQVTK
                     DDNGTIYD"
     gene            complement(15666..16580)
                     /locus_tag="VK031_06710"
     CDS             complement(15666..16580)
                     /locus_tag="VK031_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLS53645.1"
                     /translation="MIIVFSDIHIHNSSRFGSAEDRLNDHIKCLKLILRAAVNRDVDA
                     VVFCGDMFEKHGTVSADVLVPVMEVFSDFENKRIPFVAISGNHDMTKKNYLDKPSPTL
                     LQVLNESFEYFKLIDNDFFDHRRYRFTGIPYLSSKEDISRALDNVELHPDKENILLMH
                     NTPETDNVMIDVDIDMYDERIQQFDQVFNGHIHDRFSEGKFHVIGNPMQRTLNDVGVE
                     KGVYFYADGKMRFVSMNKHLPEIKITSDPKSEPEEGVIKIVKPPEMEEVDAEIVFEDY
                     DQLLEDYLEVADTELDKGKLLGVIKSYL"
     gene            complement(16577..17035)
                     /locus_tag="VK031_06715"
     CDS             complement(16577..17035)
                     /locus_tag="VK031_06715"
                     /inference="COORDINATES: protein motif:HMM:NF017578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HLS53646.1"
                     /translation="MSKISLEAVITDKGLLKPKEHLKIKNIFRARGWVGKSVILEVSK
                     YYRNRTNRQNRYIWGVMVPIIQRWILETQGEKVTKDQAYMYLRMGVLEEKPVITEIAG
                     TEVITFESKRFSRMNTKEFSEAIEEVIAIMAERGCVIPLPNEENLITDFL"
     gene            complement(17099..17983)
                     /locus_tag="VK031_06720"
     CDS             complement(17099..17983)
                     /locus_tag="VK031_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53647.1"
                     /translation="MAGFDPKKISGLTEKINKAKRGSTKILEARQLETDRPRQIRLLK
                     PLPNQGSEWDYAIEGLGYWINQKYYRSPASIGEECPIEDEIQKARKLNKPSINSLLSD
                     WRNFSRDYAFYMPIVPVKPNIDLDTGEVVSSEIAGPERVLKAPISLMEGIQEIVQSPS
                     TIAGSKGWGIFHPELGYSITIKKTGSGKDTRYSAILDPSRVPVSEEHYKPYDIVGHFK
                     QELKPNEELRAAIRSYLYGEEYDVEDLEEGDTEPKKKQATKSKKTEKVEDDEVLDIDN
                     LEDSDDELLGTLMEGIDL"
     gene            complement(18005..>18868)
                     /locus_tag="VK031_06725"
     CDS             complement(18005..>18868)
                     /locus_tag="VK031_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HLS53648.1"
                     /translation="EFRVQYKELAEMVRYVKKSFVAPEGKHLLQFDLSQAELRFMAEF
                     SKDETMVEAYKDKKDLHAIGAAHSMDIPIEEWYGLDEDTKSKTRFHAKARNFGLLYLM
                     SVGGYQKHLKEKYSVIISRAEAARQHEGFFRLYPGIRKYHKIYEAKAKKFGKVRNWFG
                     RQRILPNISSRDEFLRSLDIRAAINMPIQGTAGDYLTMSMVLIRMRLGTEYEYPGYDV
                     PIQVNSIHDAILAYSNKNNTKYVIREIMGVLNNLPVEEYFHRSWDNLIMESEVEISDE
                     SWADIKEIKLN"
BASE COUNT         5242 a         4192 c         2664 g         6770 t
ORIGIN      
        1 tactttgtca accaattcgt ttttaatcag tttatgcgct tcctgaccac ctttattttt
       61 gtggtaggtc gcaaactcgt ttaacttttc gtgtaattca accaagttct ctgtatttag
      121 taaatccata ataataactg cacacaacac tgtataaaat caagtggggc tgtgtgctta
      181 ttttgaccac ttctgctgtt taattagttt tgtgcaagtc gggacgtgtt tgcagttcaa
      241 tccccacctg cttttatact tttaccgttc tttcaattct caactattcg actttttcga
      301 atagttcaaa aaaaaaaacc agaacccctg tccctaaatc caacactggt taataatatt
      361 tggggttctg gcttaggagc aaggggcagg atttgaacct gcaatttgta cgctcaactt
      421 gagtgccacc ccatggattg cgagtttgta tcttacgttt accacgctgc acgtatcatt
      481 aggcttttag cgtctaccat ttccgccacc cttgctataa atggcctgag cttccccagg
      541 cctacattta aggaaattaa gcaggttcaa accctgcaac tttaaaatct ttagtacact
      601 cccgacaccg ggcatcaaat tctgtatctt caccatcctc tacctccgta aattgtgcaa
      661 accgatcagg attaaagagt gtagagtatc ctacgaaaca gtggggacat ctaacctcgt
      721 agattatatt gacccaccct ttaatggttt tcacatctgc catatcactt catttagata
      781 gtaaatatac gcaaataata gcgatattag taatataata atgatataag atagcatgga
      841 acttattttg tctattctat atcttatatc agctaatgca tatcctaaga tgaatcctac
      901 taccgataaa taaactaacc caatcatgtt attttgtaat ttataatgtt tctatcctct
      961 ttcaattgct tctctaattt accccacgtt atggggaact ttatccgggc aaaatcttga
     1021 tttatactga tccggtgttt accacattct gataccagat atagtaaagt accgtctatt
     1081 agatataacg taaataccgt agattttgat atgctcatgt catataaatt gttttgtgta
     1141 tcgggtttaa acttaacttt accttccgtt gacaacgttc tactcattac acgtaatttt
     1201 ctatgttagt gattttatct tcgcctatac gtaaatcaac tccggtagct agtccatttc
     1261 tcaataaatg tcttatagct ccccaggtag ctccattggc taccccttcc ttcgtgaata
     1321 acctaaggat catatcttcg tagcttagta ttattaagtt gttgcgaacc acctcaatag
     1381 tccaagaact atccaatggg atgttttcta agtgtttgat tactgttttc attacaagta
     1441 ttttttgatg tctacattag tgtatagatt accgacccat agtgaaacac gttcactatc
     1501 tagtaatgtt tgttttagat cctcccacct acaaggaaac ttaataggcg tgtacgacgg
     1561 tgtaaaactg attgtgtatt cttccacgtc tgtaaagact tgaatagtag gccaagatac
     1621 ataccgcaca ccccataagt ggtttaaccc cattaatgtt accgcattta atttcatttt
     1681 atactcttta aaaatgtttt caaatctttg atatcctccc gactatccac ctttaggata
     1741 gatttggtga ttgaaccatc cataatacca cttttcagag cttcccatgt gatcggcgct
     1801 ctaaggtaag tatcttgcct aatgtacaaa tgatacacgt gagagctatc ccgggctgtt
     1861 agcgatacag catcccagtt aatactttct accgcccact ccatgttagg atccatattc
     1921 tttacaacat gtctaacatc attgtacctc agatactctt ttaaatcagg taacttctct
     1981 gacctagtac catccgggtt ctggatcaat actttcgggc ttacgattct atcgttggcc
     2041 aggatctcct ttttaatact cccccaatca aacgacatta agttcgcctg cctggtgttt
     2101 atatctaaaa tgaaatgaaa gcccttctct acacaactca tttgtataga ggtgctgata
     2161 ctatctaccg accattgagt agattcgggc atgtctgtta aaacttgcaa tgacttcatg
     2221 tttctaagtt tttttttaga tgaaaaataa ctctacagcg atttccttca atcgctccat
     2281 tacgtaatct gaaacttcca gttcgtcccc ttcatagaat acttcaatat cgtccactaa
     2341 cagatccagc ttattgtcat aagtgatcct gtaggtgtag tagatattgt aagcgtagtc
     2401 atttcgctca atgtgaagga catctgattt ccagtaattt agttctaata cttctatttg
     2461 gtgacttgct actaacataa taatacaatg tttaatttat ggtatttatt gcgtgaagtt
     2521 gaataatttt tataaaatac ggtggcgtat ttttcaaata gctaaaactg gttttccaaa
     2581 tgaggatttg gttattagct tatttaggta ctataatgtc ttattagggg tatttattgc
     2641 gtgaagttga ataattttta taaaatagga tttttaggca aaatcgatta catggaattt
     2701 tgcgggccat tgggctaaat gctaggctgg tctttgattg tagcgctttg ataacaggat
     2761 ccaggataaa aggatccagg ataaaaggat caaaggtaac caggatccag gataaaagga
     2821 tccaggataa aaggatccag gataaaagga tcaaaggtaa ccaggatcca ggataaaagg
     2881 ataacaggat ccaggatcaa aggataacgg atccaggata aaaggatcaa aggataaaag
     2941 gataacggat cctaacacca ggacaaaaaa aaaagcgtcg atgagtaact actcatcgac
     3001 gctttttatc tctacaatat cagtactaca gcaaaaagct gttaaatagt actatctctt
     3061 gttcatcctc acatgtggtg atttcgttta ctttttccat taaatcaccc tctggtacga
     3121 tatacccaga tactcccgta tttgtcgctg aacctactaa aaatattata ggaccagcac
     3181 aaaaataatc agtacaatat ttttttatac tctctcggta tggatgcaca tcattataat
     3241 taagccatcc accagctatt actttttcta ttatctcgta tagtttcata tcactagatt
     3301 tatgattcta aaaaatgtat gatatcagac atcatactct cccaggccac ccagacagcc
     3361 cagttcttgt aagatacttc ttcatttatc ggacaataag gattaactat ctccaggccc
     3421 tcattattga ggtagtcaaa ataacggtgt atgcgaccgc tattttggtt ataaattttt
     3481 tctgtctcat tataatacgt gagtcccggg ataccagact ctgcagagat ataatcatcg
     3541 ggccacctcc ttaaatcggc ccatggtaca ccaccagagt ttagtaaatt gttgaatagt
     3601 tccggatggc tgcaagtagc taaaaaatct tttttaaact tcattttgtt agttttttgt
     3661 ttagtaaaat tattattctg aacataccca gagatccata tcctggtaca gataaccatt
     3721 atctgcacac tctgcattat ccaatatgtg atcccaagca aaccagtatt catcctggtc
     3781 aggaccatct tttaaaatct ctaattcgtc gattaactcc gatgagttaa ttaaaatatc
     3841 tgcgtgatct tctgcaaatt tttgtggtat gtatacacct aaactcccat caattaataa
     3901 cttcattttg ttgctttttt ttttgttgtt taaaaattta atcctctggg tatgttaatg
     3961 ctatgtacac catacccgca atgcctacca gtaggcagac agttacaaat attactgcta
     4021 tggaatataa aacttcactg taattcattt tgctgttttt tttttgttat caatgattca
     4081 aatataagcc cactatttag aacaaacaaa tattattgac acatattttt atctgtaata
     4141 tacatcaacc tctataccag ttcattaggc ccaaaaatat ttttcaaact ttttccgatc
     4201 catagacaat ataaccgatt tttgacattt cagatttata tttctttaat gtataaagag
     4261 ttgataatca gtacaaccat tgactatcaa cgctttatac attacacttt tacctatgta
     4321 aattccaggc cggaatagct aatccgaaaa actcatattc cgacgatttt gccagattcc
     4381 gacgtttaga ttccgacaga aaaataattt ttacgaatcg ttttttaaaa ttacagctgg
     4441 tgccaaattg tatcttcctt taccgtatcc gatctgcatc cgatattcct taccaacttt
     4501 tttgagttga ccaacaccac cagatagcat aaatttatga tctatcttat atcctgaaga
     4561 ttccaattgt tcaatacgat cctggacatg ataatgggta tcaataatgt attggatttc
     4621 ccaaatcttt attggtaagc ttgatccaat ccatccagat aattcatctt cgaacctact
     4681 tatgaaagaa tagaaaatat atttatctgc atctacaggt aattctgcat ctgagtactt
     4741 atctactaat gttgaactat ttgtataatg cttcaggtac atcaaatcat tatcgtactc
     4801 aatgactacc tttaccatcc accggccata acttactatt cttacctgtg attctactac
     4861 tctaatttgt ttgatcattt ttgatgtttt taagtgttaa ttaaatgcta cttttaagtg
     4921 cctcgctaat tgatatccct tttccaagct gtccatgggt tcatcactga agatgatcgg
     4981 ataaccaaaa ccctcttcat tatcccatgt atgtagttcc acatccacag tagtatctaa
     5041 cggcaatttg tggaatagta ctacgaaatc atatgctggt ttcaatggat cccggggcaa
     5101 gctagtttga aaaatacctt tacaacccgg aaattgaacc tcaaaccgct gggagatgca
     5161 ccctctacta gatgcatcaa ttgctttatc tggatcaaaa tccagtcctt ctatccaatt
     5221 ttttatatcc tttttcatat ctcaaagatt ttctctagga attgatcact gaactcgtta
     5281 taaccctctc caaaataaga taataggttt ttcattccct ttatcagttc gtgattatcg
     5341 aatgtctcat cgtgctctga tgcaataaaa taaggttcct cacgccaaaa tattggtatc
     5401 acccatttta attgtgttgg gtacattgtc ttgataggtg tacacaaatc aagaattata
     5461 ttcagggccg tattatagtc ttcctctctt acgatctgat gaatgcggct catcactctt
     5521 ttctctacat ttgataactt tacttttctg attttcatat taacgttgtt tgatttgttc
     5581 agtagcaaat ataagggtac tattttgata tcaccaaata tttcaacacc tttttccaaa
     5641 ctttattcca tttaccttgg tacgatattg aaaaccaacc agttacaact gaaataaatt
     5701 tattcctgga taaatgccgg ccgggtaccc tggtgccggt acttaatcga aactatctac
     5761 atattggagt ttaggccggt gattatggta cgtgattgaa tcccagtact ttatacatat
     5821 gtttatgtca tatatagatt ggctgccata tggaccgctc ctgggtgagt aggccgcttt
     5881 tgtaaaaccc cagattccga cgtttagatt ccgacagatt ccgacgattt taaattacct
     5941 taggttccga cagatttttt ttaggtgaga ttccgacaaa aaatgaaggc caggtaacat
     6001 caaaaaaccc tggccttcct ggtatccaaa ttaaaccaca aaacggggta aaatgaatgg
     6061 aaaaaacccc gagtaaacaa atcacaactt ttacgtattt atgccctgtg aggaatgatc
     6121 ctgcatgggc gggatacaat ttcttagtag taaatcaaac aacttttatt gcatcctaaa
     6181 tatagctctt tttcatgtga aagtgaagga tcttcatcct aaattcctta ctataaccct
     6241 ctaccggatt atttattgta tctacttgaa attatttcac ttatctgctc aattagttga
     6301 ctgaactctc cgtttactaa tgtaataatc tgatgtacaa taacaatata ataaattata
     6361 atgatcaata atagtataat gagtagtacc gctacttggc ccagtttgcc tctcatcctc
     6421 aatgttttaa gtgttagtaa gtgttatctc gattgtcagc ctctaaccga ttaaacatat
     6481 cccgtaactg ctcctctgaa ctcattggtg attgtagtga ggccggtggc tctacttccg
     6541 gtaaatcttc aacctttacg gtcaaatcag aatataagtc aagtatctta gctcttgttt
     6601 ctattacttt gaccatagta ttaaggaatc cgtttgatcc tttgcctctc ctggtggttt
     6661 taatgcgttt taagacctct tgacctaatc ccttgccgat atccctataa tgcgtttgaa
     6721 gctgttcatc gggctctaaa gtgcctctaa aggcatttaa agcaagatcg taaacctcat
     6781 ccaactcctc taaaatagct aaccgctcta attcctggtc caccatatcc tgattcgctt
     6841 taagaaatgc atggtattga ctccttatct ccctgcgtgt aactacataa tcacggttcc
     6901 gggcattaag cttatcggtg atctctagcc aggtatggtt agtagatttt aattctataa
     6961 tcaagtctaa atcctctgta cgctcttctt cagtgcgaaa aacccgttta tgaaggggtg
     7021 agtacgatat ttccggtttc atttcaagat attttttcga ctttgtctgc atgttttgca
     7081 tgaataaata tatacttcag cctccttaaa aatagctatt tacaaacttt aagctcataa
     7141 atgactgata attaagtaag tacgcaatta atacccatgt aaaatacgaa attttattgc
     7201 tatagccaaa ataaattgaa aataacacta tttttcccag tctttttctg tcacccaccc
     7261 tttactctcg cctaccttga accttcgtcc tttgtgtttt gtgagtacct tgtaatcttt
     7321 ttgcttcggt tcccatctat agcccccgtc caccctcggt agtctctctg caccttactc
     7381 ttcactacta taccctagat ctctcattat agcttgttta ctactctttg gtatgtctgc
     7441 atcttggaac actcccctct tattatcgag caaccattta gccaactctt ctaatggtgt
     7501 gtattttagt tgattatcat ctactcccat ataaataacc tctggataat cctggtagtg
     7561 ttctacagct tccataatct cttctttgtt atctactccc catccccata aatatctttt
     7621 gtaatatcct ttacgtacta cccttaaaaa ctgcccttct ctatacctag cgcttagttc
     7681 acctgagtat gagcgtacac taactccttt agtgagtttt ttcccttcaa cggttatgta
     7741 gactgtatca ttactgaatt ttagtgcttt attagccttc ttaaatacct ttatactact
     7801 actatcgaat atgtaccctc tactattgac tagtacatac tctcccttat ggtaccagga
     7861 ttctttattg tctatagctt tttctacctg ctctggtgta cggtatagaa acttaccttc
     7921 tttaaactgg taagctatat gtctgtcgtt attcattttc tattttgttg taaaattcgg
     7981 ttagtgtcct tatcctgcgc ccatctaact tagggcttat atgtttgtag ctagctaacc
     8041 tagtagctaa gattttatct attatatgtg gacactctac acctgatccc tcgtacttaa
     8101 ctggttgatc atttatgaag cctattatac agtactctcc tttgtcatac caactatcat
     8161 ctactttctt tgctttatct acttcgtttg gggttctgaa tactggttga tcatttatta
     8221 cttgataata gagtgatctt acttttatgc tccccatacc taaaggtact tcttctgtgt
     8281 agtcagtgat gtattcttta cctgcttgat tgttttcttc ttttagattg actgtgtgta
     8341 tgtctaaccc ggattcttct ttgctaatgc gctccaatga attgtcgttt tagtgtctca
     8401 cttcttttta tgcctaactt taaatttact atctctctac tacgttctaa tacttcgttc
     8461 ctaatgtatt ctaactgttc ttcactgact gctgggtagt actttctagc tagtaaactt
     8521 atagtaggta tcttgttgtt tctactctct tttaaatcgt tatccatcat gtgatctata
     8581 tcttccctgt agtaatggat aattctattt actgtcttat ctaccatctt atctatttag
     8641 caaggtgtgt aaatatctaa tttggacccc ctgtcgatta acctgttcct acatatttct
     8701 attatctgtc ttaggtcagt gttagttcct ctttcgtact tgtactttat aatgtctggt
     8761 aagtctgtca ctctgtctgt atctgattgt atcagatgta cggtggtttt gcctcccttt
     8821 acgagttgat agtgcttgtt tctacgtacc ctacttgatt tatcgtggtt atacgtatgc
     8881 tcactatgat tagagcgctt gtagccatta tcctctaaga aactaactac ctgttttatg
     8941 tagtctctat taactaggat atctatgtcg tttacttcat ctagtaaacc tttttcaaag
     9001 aacaagtaac taccgattac ctctacgtga aaataccttt ttaataactc taccattcta
     9061 aataatttaa ataatagagg gcggtgcttc atactctcta caactatttc cgccccctat
     9121 tcaccctata aatatacgaa attaactact taaatacaag tattattgcc ctaaaacacc
     9181 agttaatagg gtgtttattc tatcttttag ctctgtaaaa tctatgtcgt ctggatcgct
     9241 cttgtatatc tgtaataaca ggtagaacat atccttagac ctggccataa gataaccatc
     9301 atcctcatta aaaactgtag ccactgtctg gtgcctatct gtgattactt tacagtctgt
     9361 gtaagtccat aatccagatg tccctgtaaa tttagattta taaggtatcg gttccctaga
     9421 tttgtactct gaccttataa cctcctctat taatttatgt agttctctac ctacatatgg
     9481 gtcagataat agtgtagcta ctctctcgtg catttatctt cttttctaat tccggtgctt
     9541 tagcaattaa atttgctcta tctaatgctg tctgaagatg gttggcatat attttagcca
     9601 ctagttcacc gctatctgta tctactatat ggtaaatact gtcaccttca cctgttttaa
     9661 tctcccattt tgccatcgat ctcctcttgt gtataattat gaaaaatagc ttctgtgtac
     9721 acgtgccgcc tatatgtagc accaaattct gatatactgt acttgtgttt tgaataccag
     9781 gccccatcct ctcctttctc ccactcggta gtcaccactc taacggtacc ttcttgatct
     9841 ctccccttct gggacgaata ccacacttta tctcctacgt ggtgtttagg ctctttataa
     9901 atatacttcc gcattctcag ctaagttttt agttaataaa tcaaatacat ctaaatgaaa
     9961 aaccccgtac cctaatatcg tctccatagg gtatgaccca ggttcggatt ccaactgtaa
    10021 accctctgga actgggttga gtaacggttt tatatctgtg atagctaact tatgatcctt
    10081 accatctgca tcctgccaaa tacaatctaa taccgtgtct ttaaattcct ttatctgaat
    10141 acctttaagt acatacaaat taccttcatc atctatacct ttaagaccgt aaggtaaata
    10201 aattgctaac tgccatagtt gtatcatatt actttatatc ctttaccatg taacgtaatc
    10261 cacgaaggtt cattgtagct ctctactttt atataacctt ctctaataag tttctctgct
    10321 gtttttctag cccggtattc tgtaacacct aaagcactga caatatccct cactagtgcg
    10381 tacatcctag cttggttaca agcatatata aacaaccata cacactctga aacactcaac
    10441 ccgtcataat ttataatgcc tacagtctta gtatttttaa ccttaggcct gggtagctct
    10501 ataatcctcc tggagacctc tatacttgtc agcataatac tctataatat atttatctat
    10561 tatctctttc tttttattta tacctaatat cctacgcacc ctactataca caatactcaa
    10621 ctctccataa cctattcttt ctaccccatc tactacctga tatacagctt ttttaacctc
    10681 tgattccatt cagtaaagat agtaaaaata atccataaaa catactaaac cagtgtatta
    10741 tctggggtca tacttatcca ccatcttata tatttgccta cttaaataat accggtactt
    10801 catataccaa tagtacgtat aagggtctaa tttatacctt ataccgctcc ttatttctct
    10861 accttttaca tctatactct tgtctctata cataatcaca tttaaatcat gggaatgtaa
    10921 agcaccggaa gagtatagaa acaccttatt atctagctcc ttatactctt ccttaaattg
    10981 ctttacagcc gggtaattat atatgaagga agaaacaaca acacccagca ctaaacccat
    11041 aacaacatta ctcattacct ttaccatccc tcagtatctt atttacatca ttaattactt
    11101 ccctgtgctt atagtctaac tcctcaaggt tatcttcttc taccccttcc tcctccttgc
    11161 atttgttgta atcccgaatc atctcattga ggatatatat tattttacgc accatgttta
    11221 tatctattta atttttcttc aaaccattct tcatcatact taacgttatg tatatccagg
    11281 tattgtctat gcctagttaa taatttgacc atatacctct tcttatctcc atatttaatg
    11341 tgaaccttcc tggtgactgc cattaaattt tcgatcctgt cctctctagt acatatatgg
    11401 tgtatatcta ctgcttcaga tcctgtaact tcgcaaggta taaagtcgtg tctatccaaa
    11461 cctaacgact ctaaatatat ccgcttgtac ttaaccataa aaataaactt ttttactagc
    11521 tctagtaact cctacatacc atagagattg cctatcctca ttgtctctac agatattaaa
    11581 gtctctatga catactacta cattctcgta cgtagatccc tgagacttat ggcatgttat
    11641 agcataagaa tatcctacat ctgcgaaccg ttccataaac gagtacatat acctccagta
    11701 cctgccttgt ttagtagtta gagccatttg tttatatgca tgtacagttg ctatgtattg
    11761 gtcctcacta tcctcatgta gtatatctat ctcattaccg taccccacat cacatctata
    11821 caccttaaat agcttatctt tatacggtaa ctctgctata ctatacgatt taacttctaa
    11881 ttcatcatta gtggtgtata atactacagg tccattgact ataggcttat ttactaccaa
    11941 ccgctcacct ggaacaatct tctgattagg tgaatcgtaa atatagtttc ttatatctgt
    12001 atttatctcg tctacctgcc tgttagtgta cgctataact ttacaataat cgggattatt
    12061 tctgtagtcc tcctgtttat acatgtctaa tatttcaggc atctctatcc tacctaaatc
    12121 attatactcc tttatgaatc tatttaccac actaggggta taaagtcctt tacgtatgta
    12181 agctgataac ggtataatac ctgatgctgt atcttgtcta ataatatcag tcaagccatg
    12241 tggcactata ttgtactgtt ctctgttact ccgcctaaac ggtgctgcta ccttgttttc
    12301 gtcccctacc ggacgtaact gtttgtagtc ccctacaaaa attataggta ccttagattc
    12361 cataaggtaa gtaaacaatt cttcattcag catagaggat tcgtcaacca ctacagcatc
    12421 gtaattccta atgttattcc ctttcgtcct gtcctgtaca aactcctgct cacccgtacg
    12481 atcattcact ttaggcatta accctaacaa actatgtatt gtagcatact cagctccagg
    12541 tactcttatg gtatctctaa gtactttaac cgctttattt acaggggcgg tcatagctat
    12601 tttaccccct ctagaatata catactcctt taaagtgtat gaaatagtaa ctgtcttacc
    12661 tgtacccgca aatccataca acagttggta cccttctggt ttatctaggt aatctaataa
    12721 ggcctctttt acctctaact gtcttggtgt tagtttcata attttttact tagtttatcg
    12781 ctttctttta agtactgcct cttcttgtgc tctatctcat attcgaatag tgatagtaat
    12841 tgaatcgctc tagagtagta aggcttcaaa tggtccgcca cattataatg gccttgtaat
    12901 tgcctaacta ccggctgaca atccatggta acatgtaact ttctacatgc atgcgtacct
    12961 aatatgagcg ctaacccatt aataagtgat aagtactctg cttttatact tgtactatca
    13021 gtatcccaaa cccctgcaaa tttcttaatt gtttccccct ctcgtatcac tatccctact
    13081 cctaatctag ttcttttgta gtagtacgaa ccatctactt caaggaagat ttttgacata
    13141 ttcctttgtt tttggggtca taaccacgta taatacacgc tgctgatctg ctaatacttc
    13201 tatcttacct ttatctataa gtttattcac ttctgtaact agcatagaat acttagcagc
    13261 aaactcttct atcgtaaggg cttctaaccc tggtatattt atactactca tattatatct
    13321 ttataactta tgttctcttt taatattgtt gatccccaaa ataaataata gttatctaac
    13381 cttaggaacc atggatactc tttattacgg tcatttacag gtaccgttac tacgtaatct
    13441 gtaatcctat tctgcctaag tagccctaaa caattactcg cccctacacg taccatatca
    13501 gcctctgctt gcttcataat attaggtatt atagaattac ttctggtacc ttctagattt
    13561 cctaggtatt ttagatgttt agtctcaatc accgtgtcaa actctttcat gaactctata
    13621 tctactggca ctatatcacc ggccagccat atagcttcgt tatgaatgcc tccagaattt
    13681 gcaacccgga taaattcttt acctgtccat tcttccataa atctgcatgc ccttctctca
    13741 ttcctattgc cctttctttt actattaatc ctagtcataa atagttccat tatcatcttt
    13801 agttacctgg ataaattcta ccccgtcggt taccttatct ttattgtggg taataacata
    13861 gatagattta tcctgataca ttttaattat ctcaaatacc atggatgtcc ctacctcatc
    13921 taaaccttca aatacctcat ctagtagtag tatattcatg gtaactgtct tagaagatat
    13981 aacttcatac agtgcgaaca atataccaat atttactctc tgttgttctc cccctgataa
    14041 agatttataa tttttatata cccccttatc ataacactcc aacaagaaag gcctagattt
    14101 cttagtctta tctatcgtaa gcctcactgc catgcctaat acttctgagt accttgtcaa
    14161 agctttattg aaggctacta gaatgctttc atatagataa gtggtaacac ccccggaacc
    14221 taaacctacc ctagaccacc aatccagata tttaatttta tcttcatact ttttagactc
    14281 taccttcaac tcagtcacta tagcctcctg cttatctatt tcatccttta tatgttctat
    14341 ctttttaata gtttcctcat attcatcctc tagttccgca tacctctcat ttaaatactg
    14401 tatccgctct tttactgttt ttaaggtata attattggtc tccactgtat tccttataga
    14461 ttccagctga tctttagcta gcctatgttt ttcttttaga gtttctagag tattccttat
    14521 atctaactgc tttttagcgg cctccatatt ctgctttact tggtacattt ctttacgcat
    14581 ctcttgtacc tgccgttcag cttctttagc tgactcttca gcttctttgg ttactctttc
    14641 tatagtatcg gaagtaacta tagatccgca cactgtacat gtatcccctt catttatggt
    14701 ggttatcttt gttactgtat cccgggtctg ctgaatactc ctctgtagtg attggtgttc
    14761 agaactataa tttatatatc gctcctctat ctctctgaaa ttatatttac tttccaccat
    14821 cttaattctc tgcctaatct tagcctctac ttcaaccact ctagcgtact ctttattagc
    14881 ctcctcatta gactttttag ctctccttcc catatcaacc tcagaatcta tacgcttatt
    14941 aatttcagct agtttagcat ttaatatctc cctagcgtgc tcctcatcct tacctaaatg
    15001 ctcgtattga tcttgtagag ctgttaacct cccctcttcc cggttaatct tattaattac
    15061 tttattgttc tcattagtct gctctctacg atcttcttgc accttatttt ttatagtatc
    15121 taaatctgat actatatgag caaggatatc ctcaaatatc ttacgtttat caccgtccgg
    15181 tgaatccatt aatctgtcag tattctgccc aaagaaaaca gagttctgga acgtggtggc
    15241 atccatacct agcttactct ccaatatgcg ctgactatcc gctaacttta cttcattaag
    15301 taagtctatc tcctttacct tatctatcat agttagacca ctgggatcta ctaaatacgg
    15361 aaacgaatgt ttatttctat acgtccttac tactttatac ggatggccat ctatctccat
    15421 atctatagat acataagtac ctttaaatcc tttgcctcta tattctttta gagttggtat
    15481 atctgaatta actacattct tagtggttct accaaataag caccagaata tagcattaaa
    15541 taaagtagtc ttaccaactc cattctcccc aactattata ttcaaccctg gcctgtataa
    15601 attataggtt agatctagta tagacccgaa acctttaata tctacctgtt ttatatctac
    15661 attcctcata aataggactt tataacacct aacaatttac ctttatctaa ttctgtatct
    15721 gccacctcta aataatcttc caataactga tcataatctt caaaaactat ttcagcatcc
    15781 acctcttcca tctcaggtgg tttaactatt ttaattaccc cttcttccgg ttctgatttt
    15841 ggatcactag ttatttttat ttcaggtaag tgcttattca tagatacgaa cctcatttta
    15901 ccgtctgcat aaaaatacac ccccttctct actcctacat cattaagtgt tctttgcata
    15961 gggttaccta taacgtggaa tttaccttct gagaatcgat catgaatatg tccgttaaat
    16021 acttgatcaa attgttggat acgttcatcg tacatatcta tatctacatc tatcataaca
    16081 ttatccgttt caggcgtatt atgcatcagt aatatattct ccttatctgg atgtagctct
    16141 acattatcta acgctctgga tatatcctct ttactagata aatacggaat acctgtgaat
    16201 ctataccttc tatgatcaaa aaaatcattg tctataagct taaaatactc aaacgactca
    16261 tttagtacct gcaataatgt aggtgagggt ttatctaagt aatttttctt agtcatatca
    16321 tggttacctg aaatagctac aaaaggtatt ctcttatttt caaaatctga aaatacttcc
    16381 attacaggta ctaatacatc tgcacttact gtaccatgct tttcaaacat gtctccacaa
    16441 aacactacag catctacatc cctatttaca gcagctctaa gtattagttt taaacacttt
    16501 atatggtcat ttaatctatc ctccgcacta ccaaacctac tactattatg tatatgtata
    16561 tctgaaaata ctattatcat aagaaatctg ttataaggtt ctcttcgtta ggtaatggga
    16621 taacacatcc cctttcggcc attatagcta ttacctcttc tatagcttct gaaaattctt
    16681 tggtattcat ccttgaaaat cttttactct caaaggttat tacttcagta cctgctatct
    16741 ctgttataac cggtttctct tctaatacac ccatgcgtag gtacatatat gcctggtcct
    16801 ttgttacctt ctctccttgt gtttctagaa tccacctctg aataataggt accataactc
    16861 cccaaatgta cctattctgc ctgttggttc tgtttctata atattttgat acttctaata
    16921 ttacagattt acccacccat cctctagccc tgaatatatt cttgattttg aggtgttctt
    16981 taggtttaag tagaccttta tcagtaatta ctgcttctaa acttatctta gacatattat
    17041 cgttattatt attaaatcta aaaataaggg cccggttaag ggcccaatcc acgctaaact
    17101 acagatcaat accttccatc agagtcccta acaactcatc atcgctatct tctaagttat
    17161 ctatatctaa cacttcatca tcctcaactt tctcagtctt tttagattta gttgcctgct
    17221 tctttttagg ttcggtatca ccctcttcta aatcttccac atcatactct tccccataca
    17281 aataactccg tattgctgca cgtaactctt cgttaggttt caactcctgc ttaaagtgcc
    17341 caacaatatc gtaaggttta taatgctcct cagataccgg aaccctacta gggtctaaga
    17401 tagctgaata tcgggtatcc ttacctgacc ctgttttctt gatagtgata gagtacccta
    17461 actcaggatg aaaaataccc cacccttttg aacctgctat agtactgggg ctctgtacta
    17521 tttcttgaat accttccata agacttatag gggcttttag cactctctct ggacccgcaa
    17581 tctctgaact gactacttca ccagtatcca aatctatatt tggttttacc ggtacaatag
    17641 gcatgtaaaa tgcgtagtcc ctactaaaat tcctccagtc actaagcaat gagttgatag
    17701 atggcttgtt tagttttcta gccttctgta tctcatcttc aatagggcac tcttctccaa
    17761 tagatgctgg agacctgtaa tacttctggt ttatccagta ccctaaacct tcgattgcat
    17821 aatcccattc agacccttgg tttggtagtg gtttaagtaa tcggatttgt ctaggtcgat
    17881 ctgtctctaa ctgtcgtgcc tctaggatct ttgttgaccc tctttttgct ttgttgatct
    17941 tttcagttaa ccctgaaatt ttcttcggat cgaatcctgc cataatctac aatttattag
    18001 ttaattaatt tagtttaatc tcttttatat ctgcccaact ctcatcagat atctctactt
    18061 ctgattccat tattaagtta tcccatgacc tatggaaata ctcctctacc ggcaagttat
    18121 tgagtacgcc cataatctct cttataacgt actttgtgtt atttttattg ctgtaagcta
    18181 atatagcgtc atgaatagag tttacctgta taggtacatc ataacctgga tactcatact
    18241 cagtacctaa ccgcatacgt atgagtacca tactcatagt taggtaatct cctgccgtac
    18301 cttgtatagg catgttaata gctgcccgga tatccaagct ccttagaaat tcgtctctag
    18361 aggatatatt aggtaatatc ctctgtctac caaaccagtt acgtacttta ccgaatttct
    18421 tagctttagc ctcgtatatt ttatggtact ttctaatacc tgggtacaac ctaaaaaatc
    18481 cttcatgctg cctagctgct tcagctctac tgataattac cgaatacttc tctttaaggt
    18541 gtttctgata gcctccaaca gacattagat acaataaccc gaaatttcta gctttagcgt
    18601 ggaacctggt tttagatttg gtatcctcat ctaatccgta ccattcttct attggtatat
    18661 ccatactatg tgcagcacct atagcatgta aatctttttt atctttgtaa gcctctacca
    18721 tagtctcatc cttactaaac tctgccatga atcttaactc tgcttggctc aagtcaaact
    18781 gtagtaagtg tttgccttct ggtgctacaa atgatttttt tacgtatcta accatctcgg
    18841 ctaactcttt atactgcacc ctaaactc
//
LOCUS       DATGSI010000434         1503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_173199, whole genome shotgun sequence.
ACCESSION   DATGSI010000434 DATGSI010000000
VERSION     DATGSI010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173199"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..201)
                     /locus_tag="VK031_06730"
     CDS             complement(7..201)
                     /locus_tag="VK031_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53649.1"
                     /translation="MWTTVYVASGIETAREIEKQLVEEGFMVQVKYFTSENGEELYEL
                     LVPRFEAEEIQLCLIELGII"
     gene            complement(247..>1503)
                     /locus_tag="VK031_06735"
     CDS             complement(247..>1503)
                     /locus_tag="VK031_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HLS53650.1"
                     /translation="NKIYDLMIDFANYAFNKSHSAAYAVIAYRTGWLKHYYPVEFMAA
                     QISSIMGDTNSVSLYIQECKRLGIDILPPDINESYHKFTVSEGKIRFGLTAVKNVGTN
                     LISAIIAARKEGRFESFIDFVERVAREDSTAMNKRAIESLIKSGAMDSLGANRAQLLA
                     IFEKTIDSVYSDRRRNIDGQFSIFDTVDNRAKKDNLPDLKEFPQKVLLSMEKEILGIY
                     VSGHPLDPYKKELDSISTITTSEIFAELEEKENNVARVKDGQKVTIGGIITAKRNMVT
                     KNNNMMAFISLEDMFGSIECIVFPQTYDRYNDLILEDKIIVVEGRISASEVEEAKIIC
                     EKIYSLDEYKLDKLYLRLNTNKPKELFDRVKKVLKQYPGDTPVYVYIENTKKAFVADK
                     SLWVNGSNKMIHELERLLGKNSVKTS"
BASE COUNT          439 a          305 c          192 g          567 t
ORIGIN      
        1 aaataattat attattccaa gttctattaa acataattga atttcttcgg cttcaaatct
       61 tggaaccaaa agctcataaa gttcctcccc attttcactg gtaaaatatt taacctgtac
      121 catgaatccc tcctctacca attgtttctc tatttccctg gctgtctcta ttcctgaggc
      181 aacgtaaact gttgtccaca taaaatctac ccccaaaact atgcttctaa aattaacatt
      241 taaaaattat gatgttttaa cagaattttt acctagtagc ctttctaact catgtatcat
      301 tttgttactt ccattaaccc aaagggattt gtcggcaaca aatgcctttt tagtattttc
      361 aatataaaca tatacaggtg tatcgccagg atattgtttt aaaacttttt tgaccctatc
      421 aaataattct ttaggtttat ttgtgttaag tcgtaaatat agtttatcta acttatattc
      481 atctaaagaa taaatctttt cacatataat ttttgcttct tccacttcag atgcgctaat
      541 cctaccttct acaactatta tcttatcctc taatataagg tcattatatc tgtcataagt
      601 ttggggaaag acaatacact ctatgctacc aaacatgtct tccaaactta taaaggccat
      661 catattatta ttttttgtta ccatatttct ttttgctgtt attataccac ctattgttac
      721 tttttgccca tcttttactc tggctacatt attttctttt tcctctaatt cggcaaagat
      781 ttctgaagta gttatagtgg atattgagtc cagctctttt ttatagggat ctaaaggatg
      841 tccagaaaca tatataccca aaatttcctt ttccattgac aacaatactt tttgtggaaa
      901 ttctttcaga tctggtaaat tatccttttt ggccctatta tctacagtat caaatatgga
      961 aaattgacca tctatattcc ttcttctgtc tgaatacaca ctgtcaatgg tcttttcaaa
     1021 aatagccagt aactgagccc tattagcccc taaactatcc atggcaccac ttttaatcaa
     1081 actttctatg gcccttttgt tcatagctgt agaatcttct cttgccactc tttctacaaa
     1141 atctataaag ctttcaaacc tgccttcctt cctggcagct attatggcac ttattaaatt
     1201 ggtgcctaca tttttaactg cagttagacc aaatcttatt ttcccttctg atacagtaaa
     1261 tttatggtaa gattcattaa tatccggtgg tagaatatct atgcctagcc ttttgcattc
     1321 ttgaatatat agggagactg agtttgtatc ccccattatg gaggaaattt gcgccgccat
     1381 aaattctact ggataataat gctttagcca acctgttcta taggctataa ctgcatatgc
     1441 tgcagagtgg gacttattaa aggcatagtt ggcaaaatct atcattagat catagatttt
     1501 att
//
LOCUS       DATGSI010000435         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_127189, whole genome shotgun sequence.
ACCESSION   DATGSI010000435 DATGSI010000000
VERSION     DATGSI010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127189"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1418
                     /gene="gcvT"
                     /locus_tag="VK031_06740"
     CDS             318..1418
                     /gene="gcvT"
                     /locus_tag="VK031_06740"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HLS53651.1"
                     /translation="MAGKKTPLYDEHLKLGGKIVDYAGWLLPIQYEGLVIEHNAVRER
                     AGIFDVSHMGLIQVEGRDALAFLDYLMTNNINRIKRNQIIYTFMCYPDGGVVDDLLVY
                     KHHKEKFLLVVNAANLEKDLEWIEENKNNYQVEIEDITEEISILALQGPEAESILQGL
                     TETDLSKIKFFHFKEDIKICNISALVSRTGYTGEDGFEIFVQSKKVEKLWEQILDFGK
                     DYGLKPAGLGCRDTLRFEAALPLYGKELSADISPIEADLKFFVKVNKPSNFIGKDALK
                     EQVDGNIKRLLVGFELLDRGIPREGYKLYKADKEIGYVTTGYMSPSLGKSIGNALIDI
                     DEAKIGNEIDIMIRNRAVKAKIIDRNFLKNNK"
     gene            1431..1814
                     /gene="gcvH"
                     /locus_tag="VK031_06745"
     CDS             1431..1814
                     /gene="gcvH"
                     /locus_tag="VK031_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HLS53652.1"
                     /translation="MKVLKDLYYTDEHEWLKVEDGEALIGITDYAQDQLGDIVYVELP
                     EVEDEISKDEGFAAVESVKAASDIYMPVDGRIIEVNEELLDSPELLNDEPYETWIVKI
                     ELTDEAQLDDLMTSEEYEEYTSEEV"
     gene            1816..>2412
                     /locus_tag="VK031_06750"
     CDS             1816..>2412
                     /locus_tag="VK031_06750"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583228.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HLS53653.1"
                     /translation="MYPYIPHTAEDIDEMLEAIGLERLEELFQDIPKDVALNRELDLP
                     SSMSEMEVRSYLESLASKNSTTDDKVCFLGAGAYDHYIPAIIDHIISRSEFYTSYTPY
                     QPEISQGTLQYLFEYQTLICNLTGMDVSNASLYDGGTALTEAALMATSVGRKKEIIVS
                     KAVNPQAREILKTYAHIQELKILELDIKDGVTDLEALEE"
BASE COUNT          885 a          324 c          526 g          677 t
ORIGIN      
        1 taatatccac agaaccgtat ttttttgaaa gaaatatgtc aattaatgag tttctatcta
       61 atttgacaag ttataaagaa aaaagccatt tttaaatatt atgtagtatt ttaccgtaat
      121 ttaaaagtat aggttatgat gaagaaagta gatatgaata aataacctag gattgaataa
      181 tattttcact tttactataa tagatttatc tactagggac ttgtagctgt atggtttcaa
      241 cttgttttac ctttgataat aaagggtaca ataaataatg attaaatttc tattgcttgg
      301 gggagaggag tgatccaatg gctggcaaaa aaacaccact ttacgatgaa catctaaaat
      361 taggcggaaa aatagtggat tatgctggtt ggctactacc tattcaatat gaggggctgg
      421 ttatagagca taatgcagta agagaaaggg caggtatttt cgatgtatcc catatgggct
      481 taatccaagt tgaaggaaga gatgccttag cctttctaga ttatctgatg actaataata
      541 ttaaccggat taaaagaaac cagattattt acacctttat gtgttatcca gatggaggtg
      601 tagttgatga ccttttggtt tacaaacatc ataaagagaa gtttttattg gtggtcaatg
      661 ctgccaatct tgaaaaagat ttagaatgga tagaagaaaa taaaaacaac tatcaagtgg
      721 aaatagaaga cataacagaa gagatatcca tattggccct acagggacca gaagcagaaa
      781 gcatattaca gggcttgact gaaactgatt tatcaaagat aaaattcttt cattttaagg
      841 aggatataaa aatatgtaat atttctgccc tggtgtctag aacaggatat acaggggagg
      901 atggctttga aatctttgta cagagtaaga aagtggaaaa actttgggaa cagatattag
      961 actttgggaa agattatggc ctaaaacctg ccggcctagg ttgtagggat accttaaggt
     1021 ttgaagctgc cctaccccta tacgggaagg aattatcagc agatattagt cccatagagg
     1081 cagacttaaa attctttgtt aaggtaaaca aacctagcaa ttttataggt aaagatgcat
     1141 taaaagagca agtagatgga aatattaaaa ggctcttggt aggttttgaa cttttagata
     1201 gaggaatacc tagggaaggg tataagttat ataaggctga taaagaaata ggctatgtga
     1261 ccacaggcta tatgagccca agcctgggaa aaagtattgg aaatgcctta atagatatag
     1321 atgaagccaa aataggaaat gaaatagata taatgattag aaatagggcc gtaaaggcaa
     1381 aaataataga taggaacttt ttaaaaaaca acaaatagga ggtattaaaa atgaaagttt
     1441 taaaagactt gtattatact gatgaacatg aatggctaaa ggtggaagat ggggaggcat
     1501 taattggtat tacagattat gcccaggatc aattgggaga tattgtatat gtagagttgc
     1561 cagaagtaga agatgagata tcaaaggatg aaggttttgc agcagtagag tcggtaaagg
     1621 ctgcatcaga tatttatatg ccagtagatg gaagaattat agaggtaaat gaagaactat
     1681 tagacagtcc agaacttcta aacgatgagc cttatgagac ctggatagta aaaattgaat
     1741 taacagatga agcacaacta gatgatttga tgacaagtga ggaatacgaa gaatacactt
     1801 cagaggaggt ataatatgta cccatatata cctcatactg ctgaagatat agatgaaatg
     1861 ttggaagcta ttggcttaga aaggttggag gaattatttc aagacattcc aaaggatgtg
     1921 gccttaaaca gggaactgga tctaccctct tccatgtcgg agatggaggt aaggagctat
     1981 ttagaaagct tggccagcaa aaattctact acagatgata aggtttgttt cttaggggca
     2041 ggtgcctatg accattatat acctgccata atagaccata taatatctag aagcgaattt
     2101 tacacatcct acacacctta ccaaccagaa ataagccagg gaaccctaca gtatttgttt
     2161 gaatatcaaa cactgatttg taatttaaca ggcatggatg tatcaaatgc ttccttatat
     2221 gatggaggaa cggcccttac tgaagctgct ttaatggcta cttcagtagg tagaaaaaag
     2281 gagataattg tttctaaagc agtcaatcct caagcaagag agattctaaa aacctatgcc
     2341 catatacagg agctgaaaat attagagtta gatattaaag atggggtcac tgacctggag
     2401 gctttggaag aa
//
LOCUS       DATGSI010000436         1441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_77293, whole genome shotgun sequence.
ACCESSION   DATGSI010000436 DATGSI010000000
VERSION     DATGSI010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77293"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VK031_06755"
     CDS             complement(<1..464)
                     /locus_tag="VK031_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLS53654.1"
                     /translation="MKDNFKDKTFYQIYPKSFNDSNGDGFGDLDGIREKLPNLQKLGI
                     DFLWISPFYKSHQYDNGYDVDDYKSIDERYGDLESFDKLVKEAKKRNIKIMLDMVFNH
                     TSTHHEWFQKALNGDKKYQDYYIFKENEGDYPTNWVSKFGGPAWKYVEKLDK"
     gene            complement(454..>1441)
                     /gene="treB"
                     /locus_tag="VK031_06760"
     CDS             complement(454..>1441)
                     /gene="treB"
                     /locus_tag="VK031_06760"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HLS53655.1"
                     /translation="FWAGVNSFLWLPAEAIFHFLPVAITWSVTRKMGATPILGIVLGI
                     TLVSPQLLNAYGVAEAAASGEIPVWDFGFFKLNMIGYQAQVIPAMLAGFALVYLERFF
                     RKIIPEVVQMIFVPFFSLLISIIIAHAIIGPIGWRIGSFISSVVYSGLTSNFRAVFAA
                     LFGFFYSPLVITGLHHMSNAVDLELIGKFGFTLLWPMIALSNICQGSAVLGVIYLDKK
                     NEKLKEVAVPSAISCFLGVTEPAMFGVNIKYRFPFLAAMIGSSLAGLFAVMTKTGALS
                     IGVGGIPGILSFPLDKWLNFAIAMVIALVVSFVLTVIFGKRQLAKKGSLNEG"
BASE COUNT          476 a          306 c          245 g          414 t
ORIGIN      
        1 tatttatcta atttttctac atatttccag gcagggccac caaattttga gacccaattt
       61 gtaggatagt ctccctcatt ttctttgaaa atatagtaat cttggtattt tttatctcca
      121 tttagggcct tttgaaacca ctcatggtgg gttgaggtat ggttaaatac catatcaagc
      181 attatcttta tatttctttt cttagcctct ttcaccaatt tgtcaaagct ttcaagatcc
      241 ccatatcttt catctatcga cttgtaatca tccacatcat agccattgtc atattggtgg
      301 gacttataaa atggggatat ccacaaaaag tctatcccca atttttgaag atttggaagt
      361 ttctccctta ttccgtccaa atctccaaag ccatccccat ttgagtcatt aaaagacttg
      421 ggatagattt gataaaaagt cttatctttg aaattatcct tcatttaagc ttcctttctt
      481 tgcaagctgt ctttttccaa atattacagt taatacaaat gataccacta aagctatgac
      541 catggcaatg gcaaagttaa gccatttgtc aagtgggaag gaaagtattc ctggtattcc
      601 tccaacccct atagaaaggg cccctgtctt tgtcataact gcaaacaatc ctgcaagaga
      661 tgagccaatc atggctgcca aaaatgggaa cctatatttg atatttacac caaacatggc
      721 aggctctgta actcctaaaa agcaggaaat agcagaagga actgcaactt cttttaattt
      781 ctcatttttc ttatcaagat atataacacc caaaacagca gagccctggc agatatttga
      841 aagagcaatc ataggccaaa gtagggtaaa accaaactta cctataagct caaggtcgac
      901 tgcatttgac atgtggtgaa gtcctgtaat aaccaaaggt gagtaaaaga agccaaaaag
      961 tgctgcaaat actgccctaa agtttgaagt aaggcctgaa taaaccacac ttgaaataaa
     1021 tgatcctatc ctccaaccta ttggtcctat aatagcatgg gctatgatta tagaaataag
     1081 aagggagaaa aatggtacaa aaatcatttg cacaacttct ggaataattt ttctaaaaaa
     1141 tctttcaaga taaaccaggg caaaacctgc aagcatggct ggaattacct gggcttggta
     1201 gcctatcata tttagcttga aaaatccaaa atcccaaaca ggaatctccc cacttgctgc
     1261 agcctcagcc acgccatagg catttaaaag ttggggtgaa actagggtaa tacccaaaac
     1321 aattcccaaa ataggagtag cccccatctt tcttgtcaca gaccaggtta tggcaactgg
     1381 caagaagtgg aaaatagcct cagctggaag ccacaagaag ctattaaccc cagcccaaaa
     1441 t
//
LOCUS       DATGSI010000437         3622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_360269, whole genome shotgun sequence.
ACCESSION   DATGSI010000437 DATGSI010000000
VERSION     DATGSI010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360269"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VK031_06765"
     CDS             complement(<1..119)
                     /locus_tag="VK031_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLS53656.1"
                     /translation="MNVENIRLINFRNYENISLEFNKDINIFIGKNGEGKTNL"
     gene            complement(214..423)
                     /locus_tag="VK031_06770"
     CDS             complement(214..423)
                     /locus_tag="VK031_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HLS53657.1"
                     /translation="MKEVRIDTDYIKLDQFLKLTNIAQTGGHAKFLILDGLVKVNDKI
                     CLKRGKKLRKGDIIEIGQEGQYVIK"
     gene            complement(434..1540)
                     /gene="dnaN"
                     /locus_tag="VK031_06775"
     CDS             complement(434..1540)
                     /gene="dnaN"
                     /locus_tag="VK031_06775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HLS53658.1"
                     /translation="MKIKVNQKELAKHINKAQKGISSRSTLQILDGILLEAKDNRLKL
                     TGTDLDLSIETYLECEVLEEGSIVANSRIFGDIVKSLPDSEIMLKVEDNNINMTCENS
                     EVNISGNSAEDYPDLPLIIEKNSFKIPMDIFKETTRQVVFATTQDETRPALTGVLLEL
                     NKDTISFVALDGYRLALKKLPIETNLDTKLIIPGRSLNEINKILEETEEDMSITISLG
                     YVIFKLGNTTIYSRLLEGQFFNYKDIIREEHETSAIVNKRELQNSLERASLLAREEKA
                     NLVKLNISDNRIKIASNTEIGNVNEEITSTQEGEDLNIAFNSRYILEGIRAIDSENIK
                     LNFMGNLNPCIMQGIDDENYTYLVLPVRLAQEDF"
     gene            complement(1712..3046)
                     /gene="dnaA"
                     /locus_tag="VK031_06780"
     CDS             complement(1712..3046)
                     /gene="dnaA"
                     /locus_tag="VK031_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HLS53659.1"
                     /translation="MSQELDNIWNDVLNIIKVELTEVSYNTWLKTIVPYNMTNNTIVL
                     AAPNDFTKGILEGRYLNLIKNAIKEVTKKEYLIKFIIPGEEHSIDSGQVSVQESIDNN
                     HRAQLNPKYSFNTFVIGNSNRFAHAACLAVAEAPAQAYNPLFIYGGVGLGKTHLMHAI
                     GHYILSQNPNTRVVYVTSEKFTNELINSIREYRNEEFRNKYRNVDVLLVDDIQFIAGK
                     EGTQEEFFHTFNALYEANKQIIISSDRPPKEIPTLEDRLRSRFEWGLIADIQEPDYET
                     RIAILRKKANIENIEVPDEIMHYIANKIKSNIRELEGALIRIVAYSSLTNKEITIELA
                     EEALKDIISDNKPRVITVDLIKKEVSKNFGIKMEDFNSKKRTRAIAHPRQVAMYLTRE
                     LTDLSLPKIGDEFGGRDHTTVIHACDKINKDIETDKNLKEKIDYIIETLKGS"
BASE COUNT         1134 a          637 c          481 g         1370 t
ORIGIN      
        1 attaggttag tctttccctc gccatttttt ccaataaaaa tatttatgtc cttgttaaac
       61 tctaaactta tattttcata gtttctaaag tttataagcc ttatattttc aacattcaaa
      121 aattacaccc cttaaggcaa tacaaacatt ataccccatt aatatactat atcatataat
      181 ggggtttgaa ataaatgttc tgtattctgc tacctatttt attacatatt ggccttcctg
      241 cccaatctct attatatcac cttttcttaa ctttttacct ctctttaaac atatcttatc
      301 atttaccttt actaatccgt ccaaaattag aaacttggca tgaccacctg tttgagcaat
      361 attggtcaat tttaaaaact ggtctagctt aatatagtca gtatcaatcc ttacctcttt
      421 cattataaca ctcctaaaaa tcttcttggg ccagcctaac tggcaacact aagtaggtgt
      481 aattttcatc atctatcccc tgcataatac aaggatttaa atttcccata aaatttaatt
      541 ttatattttc cgaatcaatg gcccttattc cctccagtat atatctagaa ttaaaggcta
      601 tatttaaatc ttctccttct tgggtggaag taatttcttc atttacattt cctatttctg
      661 tatttgatgc tatttttatc ctattatcag aaatatttag cttaaccaga ttagccttct
      721 cttctctagc caaaagggag gccctttcta aactattttg taattccctc ttatttacaa
      781 ttgctgaagt ttcatgttct tctctgatta tatctttgta gttaaagaat tgcccctcca
      841 ataatctgga gtaaatagta gtattcccca gtttgaaaat tacatatcct aaagatatgg
      901 taatactcat gtcttcttct gtttcttcta gtatcttatt aatttcattt aaggatctgc
      961 caggtataat tagcttagta tctaagttgg tttcaatagg caattttttt aaggctagcc
     1021 tatatccatc caaggctaca aaggaaatgg tatctttatt taactccaat aatacccctg
     1081 ttaaggctgg ccttgtttca tcctgagttg ttgcaaaaac tacctgccta gtagtttcct
     1141 taaagatatc catagggatc ttaaaggaat ttttctctat tataagaggc aggtctggat
     1201 agtcttcagc tgaatttcca ctaatattta cctctgagtt ttcacaggtc atatttatat
     1261 tgttgtcctc aaccttcagc ataatttctg aatcaggcag acttttaaca atatcaccaa
     1321 aaattctaga attagccaca atagagcctt cctctaaaac ttcacattct aaatatgttt
     1381 ctatacttaa atctagatct gtacctgtta attttaacct attatccttt gcttccaaca
     1441 ggatgccatc taagatttgt aatgtacttc tagatgaaat tcctttttgt gccttattaa
     1501 tatgttttgc caattctttt tggtttactt ttattttcat ttttatcctc ctcgctagat
     1561 agatataaag atatactgaa ttagtagtaa tagtagtagg tcctgttatt atgtggataa
     1621 gttaaagtta atgttaaagt taaagcttac ccgatttaat aacatgttga taagtctaga
     1681 ataaaatata ctttatccac atggtgataa attaagaccc ctttagtgtc tctattatat
     1741 aatcaatttt ttcttttaga tttttatctg tttcaatatc tttatttatt ttgtcacagg
     1801 catgaataac tgtggtatga tccctaccac caaattcatc acctatttta ggtaaagata
     1861 aatcagttaa ctccctggtt aaatacatgg caacttgcct agggtgggca atggccctgg
     1921 tccttttttt ggaattaaag tcttccatct ttatgccaaa gtttttggag acttcctttt
     1981 taataagatc tacagtaatt accctaggtt tattatcaga tataatatct tttaatgctt
     2041 cctctgccaa ttcaatagta atttctttgt tggttaagga agagtaggct acaatcctga
     2101 ttagggcacc ttctaattct cttatatttg atttgatctt attggctata tagtgcatta
     2161 tctcatcagg aacctcaata ttttcaatat tggccttctt tcttaatatg gcaattctag
     2221 tttcatagtc tggctcctga atatctgcaa taagtcccca ttcaaatctt gatctaagcc
     2281 tatcttctag ggtagggatt tccttaggtg gcctatctga agatataata atttgtttat
     2341 tggcttcata gagtgcatta aaggtgtgga aaaattcttc ctgggtacct tccttacctg
     2401 ctataaattg aatatcgtct accaataaaa catctacatt tctatattta tttctaaatt
     2461 cctcatttct atattccctt atagagttaa ttaactcatt tgtaaacttt tcactggtta
     2521 cataaaccac cctagtgtta ggattttggc tcaatatgta atggcctatt gcgtgcatta
     2581 agtgggtttt acctagtcct acgcctccat aaataaacag agggttatag gcctgggcag
     2641 gagcctcagc tacagctagg caggcagcat gagcaaacct gttactatta cctattacaa
     2701 aggtattaaa agaatattta gggtttaatt gggccctatg gttgttatct atgctttcct
     2761 ggacagatac ttgtccacta tctatggaat gctcttctcc aggaataata aatttaatca
     2821 gatattcttt tttagtcacc tctttgattg catttttaat taagtttaaa tatctcccct
     2881 ctaaaatccc tttagtaaaa tcatttggtg cagctaaaac aatggtatta ttagtcatat
     2941 tgtagggaac aatggttttt aaccaagtat tgtaactaac ttcagttaat tctactttaa
     3001 taatatttaa gacatcattc caaatattat ctaattcttg agacatactt tgacctcctt
     3061 taaaaagata atggctatat aaaacaatta tcaacatact tattcacata aaaatagggt
     3121 aaaataattt aaataagaaa taaaaagaca taaaatatat gtggataaat ttatccagaa
     3181 tatgttagta tagtatttat agcatatttt atccaaatta tccacaggaa cagtaaaaaa
     3241 gaatgttaat attaaatgtc ttattatcaa ctattgacca agtttaaaat aattattcaa
     3301 taattaccca caaattatcc acaatctgtg agtagtttgt aatatattaa aagatttcac
     3361 ataatatcca catgtgtttt aataatatca aaaatataac ttagattcaa taaaattatc
     3421 cacaagatgc acaacttgtt aacaattata cttgtgaaaa agtgaacatt taatattctt
     3481 gacatactat tgactaataa atataatcaa tagtagtatc tgtcattatg cattggtctt
     3541 aaattagcca actagtgtaa ttaagaaagt tatttacttc ttaatttggt atagaggact
     3601 taaggaggtg tactggtgaa aa
//
LOCUS       DATGSI010000438         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_311637, whole genome shotgun sequence.
ACCESSION   DATGSI010000438 DATGSI010000000
VERSION     DATGSI010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311637"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VK031_06785"
     CDS             complement(<1..1157)
                     /locus_tag="VK031_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HLS53660.1"
                     /translation="MENVEYMLKSLKMINNNEYDLTKLPNYKGSSTIDINIKETVEEI
                     FEERKFIHETKEILNNEYYLDQVIEKLLVLVGETMNTNRIGVAYVDYNRGKIIAEHGA
                     FDYGNILLGSGFAVNIKDTSLSRIIENKKPVIYNDLEKEAENRKVSSALKLILREGIK
                     SNLTIPLIKNDVVFGFIFFSSSKKKNYNEKDLNLAVKISQEITGILNTSYFIKKMFST
                     MTNAFAHLVEKRDDETGFHLVKMREYSKLIAEQLLNHQNKYYKVTPGFVKDIYDYASI
                     HDIGKIGVPDKILKKAGPLTQSERQIMEEHTTIGAEILRGIEKDLKIFNQNYFQIPIE
                     IAESHHERWDGKGYPQGLKGQEIPLAARIVAIADVFDALSSKRVYKESYGF"
     gene            complement(1268..1492)
                     /locus_tag="VK031_06790"
     CDS             complement(1268..1492)
                     /locus_tag="VK031_06790"
                     /inference="COORDINATES: protein motif:HMM:NF019485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1657 domain-containing protein"
                     /protein_id="HLS53661.1"
                     /translation="MTVKNKLETALASAKGLAADLKTFSLDTDDQNAKQMFETLSTDA
                     DNIAKMIENRVNHLKNITPESTEQSDLKQF"
     gene            complement(1671..2198)
                     /locus_tag="VK031_06795"
     CDS             complement(1671..2198)
                     /locus_tag="VK031_06795"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084191859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2049..2051),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLS53662.1"
                     /translation="MERLVGRKAPDFTMKTCLGDGSGFGEVSLKDYEGKWLVMFFYPL
                     DFTLIUPTELTGYSDKVGDFNKLNAEVLSVSTDSEHAHKAWINSSLGKLNYPMASDIT
                     MSVSRDYGVLIEEEGIALRGLFIIDPEGIVRYSVVHDLGVGRSVGETLRVLKALQSGG
                     LCPVDWNEGDELLEE"
     gene            2360..2584
                     /locus_tag="VK031_06800"
     CDS             2360..2584
                     /locus_tag="VK031_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HLS53663.1"
                     /translation="MKELLDRINYLANKSKKEGLNEKEKAEQARLRQEYIKHFRGAFK
                     DTLMNLKVVDPMGNDVTPEKLLKEQGKKKN"
     gene            complement(2609..>3272)
                     /locus_tag="VK031_06805"
     CDS             complement(2609..>3272)
                     /locus_tag="VK031_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HLS53664.1"
                     /translation="KPAMRLSALMKLPVTYVLTHDSIGVGEDGPTHQPIEQLAMLRST
                     PNFLIFRPADARETAAGWYTALTREDGPTGLALTRQGLPLLEGTGKGALKGGYIVRKE
                     KGDLDLILIGTGSEVQLAYKAAEVLEDKGINARVVSMPSWLLFEEQSDEYKEGVLPKK
                     VTKRLAIEAANPLGWHKYVGSEGKVIAMEGFGDSGPGEELFEKFGFTVDNVVEEALKL
                     VE"
BASE COUNT          976 a          633 c          448 g         1215 t
ORIGIN      
        1 tcaaaaccat agctttcctt atatactctt ttagaggata gggcatcaaa tacatctgct
       61 atggctacaa ttcttgcagc caaagggatt tcttgcccct ttagcccttg gggatatccc
      121 ttgccatccc accgttcatg atgactctca gcaatttcta tgggaatttg aaagtaattt
      181 tggttaaata ttttaagatc tttctctatt cctcgtaaaa tttctgcccc tattgttgta
      241 tgttcttcca ttatctgtct ttcactctgg gttagtggcc cagccttttt aagtatttta
      301 tcaggaactc ctattttccc tatatcatgg atagaagcat agtcatatat gtcttttaca
      361 aatcctggag taactttata atatttattt tgatgattta ataattgctc tgctattaat
      421 tttgagtact ctctcatctt taccaaatga aaaccagttt catcatctct tttttctact
      481 aaatgtgcaa aagcatttgt catggtggaa aacatctttt ttatgaaata tgaagtattt
      541 agtattcctg ttatttcttg agagatttta actgctaaat tcaaatcttt ttcattataa
      601 ttttttttct tagatgaact aaaaaatata aaaccaaaaa ctacatcatt ttttattaaa
      661 ggaatggtta gattggattt aatcccttct cgcaaaatta attttaaggc agaagaaact
      721 tttctattct ctgcctcttt ctctaaatca ttataaataa caggcttttt attttcaata
      781 atcctactta aactagtgtc tttaatattt acagcaaaac ctgaccctaa taaaatatta
      841 ccataatcaa aggctccatg ctctgcaatt atttttcccc tattataatc tacataggca
      901 actcctatac ggttagtatt catagtttct cctactaaaa ctagtaattt ttctataact
      961 tggtctaaat agtattcatt atttaagatt tccttagtct catggataaa ctttctttct
     1021 tcaaatattt cctctacagt ttctttaata tttatatcta tggttgagga acctttataa
     1081 ttaggtagtt ttgttaaatc atattcatta ttgtttatca ttttcaagct tttcagcata
     1141 tattctacat tttccatatt tgttcccccc aatagtttgt ccactttata taataccata
     1201 tttcctttaa aggttgtatt tcttttatta atcaaaatca aagaagtgcc cttaggcact
     1261 tctttcttta aaattgtttt aaatcgcttt gttctgtaga ttcaggtgtt atatttttaa
     1321 ggtggttaac tctattttct atcattttag caatattgtc agcatctgtt gataaagttt
     1381 caaacatctg ctttgcattt tgatcatcag tatctaagga gaaggttttt agatctgctg
     1441 ctaagccttt tgcactggct aaggctgttt ctaatttatt ttttactgtc accaattatc
     1501 cctcctaaaa aagtttttca aaattagtat ccctaatatt aatctaaata taagaagttc
     1561 ttttgaataa ttctttccta attttgattt aattttctaa gaaataatgg aaataattgg
     1621 ttttaaaaat caaaagccct taaatcaaag gatagattta agggcttttt ttattcttct
     1681 agcagctcgt ctccttcatt ccaatcaact ggacagagac cacctgattg tagggctttt
     1741 aataccctta aagtttcccc tacgcttctt cctacgccta gatcatggac tactgagtat
     1801 ctaactattc cttctggatc tattataaat agtcctctta gggctattcc ttcttcctca
     1861 attaatacac cgtagtccct tgaaacagac atggtaatgt ctgaagccat agggtagttt
     1921 aatttgccta agctggaatt aatccaggcc ttatgggcat gttcactatc tgtacttact
     1981 gataatacct ctgcatttag tttattaaag tcaccgactt tatcactgta accagttaat
     2041 tctgtcggtc agattaatgt aaaatctaat ggatagaaaa acattaccaa ccatttacct
     2101 tcataatcct ttaaagatac ttcaccaaaa ccactaccat cacctaggca ggttttcata
     2161 gtaaaatctg gtgcttttct acctactaat ctttccataa actaacctcc tattttatct
     2221 tagttgtaga atcaactaat ttatatatat ccaattataa tgaatttaaa cttgaaataa
     2281 aaataatttc taatcatttt tttaacttaa tatatatatt ttgtttaaaa aatgatataa
     2341 ttattctcga ggtggtattt tgaaagaatt actagatcga attaattatt tggccaataa
     2401 gtctaaaaaa gaaggcttaa acgaaaaaga aaaggcagaa caagccagac tgaggcaaga
     2461 atatattaag cattttaggg gtgccttcaa agataccctg atgaatctaa aagttgtaga
     2521 tccaatgggt aatgatgtta ctcctgagaa attactgaag gagcaaggaa aaaagaaaaa
     2581 ttaaaaagtc ccccaagcgg ggactttttt attctacaag ttttaaggct tcttctacca
     2641 cattatctac tgtaaacccg aatttttcaa acagttcttc gcctggtcca gaatctccaa
     2701 agccttccat ggcaattact tttccttctg aacctacata tttatgccag cctaaaggat
     2761 ttgctgcttc tattgccaac cgttttgtaa ccttctttgg tagaactccc tctttatatt
     2821 cgtctgattg ttcttcaaat agtaaccaag atggcatact aaccactctt gcatttatac
     2881 ccttatcttc taagacttca gcagccttat aggctaattg cacttctgaa cctgttccaa
     2941 ttaagattaa atctaaatcg cccttttcct tccttacaat atagccacct tttagggcac
     3001 ccttgcctgt accctctaac aatggtaagc cctgtcttgt taaggctaat cctgttggcc
     3061 catcttctct agttaaggct gtataccagc cagctgcagt ttctctagca tctgcaggcc
     3121 taaagataag gaagttaggc gtagatctta acatggccaa ttgctcaata ggctgatgag
     3181 taggtccatc ttctcccact cctatactat catgggttaa gacataggta actggcagct
     3241 tcattagtgc tgatagcctc atagctggtt tc
//
LOCUS       DATGSI010000439         1544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_692128, whole genome shotgun sequence.
ACCESSION   DATGSI010000439 DATGSI010000000
VERSION     DATGSI010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692128"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VK031_06810"
     CDS             complement(<1..191)
                     /locus_tag="VK031_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53665.1"
                     /translation="MKNKNLKKKRRVPELRFPEFSGEWEEKRLGEVLEFKNGLNKAKK
                     YFGHGTPIINYMDVNKNKH"
     gene            complement(202..>1544)
                     /locus_tag="VK031_06815"
     CDS             complement(202..>1544)
                     /locus_tag="VK031_06815"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HLS53666.1"
                     /translation="QEEVSKLLTDQLIELRGYMIEPKYLFSTMIEEIKKGESGKFDVE
                     YLLEAINSIQESTLGQDSQETFENLFDDMDLSSTKLGRTVKSRSELMAKILVLVNEIS
                     FNQDETEIDILGDAYEYLIGKFAANAGQKAGEFYTPQEVSTVLAKIVALGKDEIKDVY
                     DPTCGSGSLLLRVNKEAKVGKFYGQELNSTTYNLARMNMLLHEIHHSKFDIQNADTIE
                     EPQHMDMTFEAVVANPPYSSKWSSAKKFEQDERFSGYGRLAPKSRADYVFVQHMIHHL
                     ADNGTMAVVLPHGVLFRGGAEETIRKHLIKDKNYLDAVIGLPENLFYGTGIPTCILVY
                     KKCRLEDDSVLFIDASKGFEKVGNKNTLRLEDIEKIIDTYAHRKEIDKYSHNATLAEI
                     EENDYNLNIPRYVDTFEPDPRVDIDKVKNRINEIDKEIAEIDQEINGYLKELGL"
BASE COUNT          386 a          318 c          223 g          617 t
ORIGIN      
        1 aaatgtttat ttttgtttac atccatataa ttgattattg gagtcccatg gccaaaatat
       61 ttttttgctt tgtttagacc atttttaaat tctagtactt cccccaacct tttctcttcc
      121 cattccccac taaattcagg aaatcgtaat tctggtactc ttctcttttt cttcaagttt
      181 ttgtttttca ttgctaaccc cctataatcc tagttctttt aagtatccat ttatctcttg
      241 atctatttct gctatttcct tatctatttc atttattcta ttttttacct tatctatgtc
      301 cactctagga tctggctcaa aggtgtctac atatctagga atattaaggt tatagtcgtt
      361 ttcttctatt tctgctaagg ttgcattatg ggaatatttg tctatctctt ttctatgggc
      421 ataggtatct attatctttt ctatatcttc tagtcttagt gtatttttat ttccaacctt
      481 ttcaaatccc ttagatgcat ctataaataa tactgagtca tcttcaagtc tgcatttttt
      541 atagactagg atacaggtgg gtattcctgt tccataaaat aggttttctg gcaggcctat
      601 tactgcatct aggtaatttt tatcttttat aaggtgtttt cttatggtct cttctgctcc
      661 tcccctaaac aataccccat ggggaagaac tactgccatg gttccattat ctgctaggtg
      721 gtggatcata tgttgaacaa acacataatc tgccctactt ttaggtgcca gtcttccata
      781 gcctgaaaat ctttcatctt gttcaaattt cttggcactg ctccattttg aagaataggg
      841 aggattagcc actacagctt caaaggtcat atccatatgt tggggttcct ctattgtatc
      901 cgcattttgt atatcaaatt ttgaatggtg gatttcatga agtagcatat tcatccgagc
      961 taaattatag gttgttgaat ttagctcttg tccataaaac ttccctacct tggcctcctt
     1021 gtttaccctt aaaagcaagg aaccagaacc acatgttggg tcgtatacat cttttatttc
     1081 atccttgccc aatgccacta ttttagctaa tactgtactc acttcctggg gggtataaaa
     1141 ttctcctgct ttctgtcctg cattggctgc aaacttccca attaaatact cataagcatc
     1201 ccctaatatg tctatttctg tttcatcttg gttaaaggaa atttcattta caaggactaa
     1261 tattttggcc attagttcag atctagactt tacagtcctg cctaattttg tagaggaaag
     1321 atccatatcg tcaaataagt tttcaaaggt ctcctgggaa tcttgtccta atgtggattc
     1381 ttgaatacta tttatagctt caagtaggta ttccacatca aattttccac tttctccttt
     1441 tttaatttcc tctatcatag ttgaaaacaa gtattttggc tctatcatat agccccttaa
     1501 ctctattaat tgatctgtaa gaagtttact tacctcttct tgtt
//
LOCUS       DATGSI010000440         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_454076, whole genome shotgun sequence.
ACCESSION   DATGSI010000440 DATGSI010000000
VERSION     DATGSI010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454076"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /gene="atpA"
                     /locus_tag="VK031_06820"
     CDS             complement(<1..1285)
                     /gene="atpA"
                     /locus_tag="VK031_06820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HLS53667.1"
                     /translation="MELRPEEISSILKEQIKKYEKRLDLVDIGTVMQVGDGIARIHGL
                     EGCMAGELIEFPGEVFGMALNLEEDNIGCVLLGSDDNIKEGDTVKRTERIVEVPVGEE
                     LIGRVVNALGHPIDGKGEIRAERFRPIERIAPGVITRKSVHEPLQTGIKAIDSMFPIG
                     RGQRELIIGDRQTGKTTLAVDTIINQKDEDVICIYVAIGQKRSTVAQIVDVLERHGAM
                     DYSIVVSSTASELAPLQYIAPYAGVSMAEEFMEQGKDVLIIYDDLSKHATAYRAMSLL
                     LRRPPGREAFPGDVFYLHSRLLERAAKLDDKYGGGSITALPIVETQAGDISAYIPTNV
                     ISITDGQIFLETDLFFAGQRPAINTGLSVSRVGGAAQIEAIKKVAGQLKLELAQYREL
                     AAFAQFGSELDAGTRERLDQGERMMQILRQPQYSPV"
     gene            complement(1305..1847)
                     /locus_tag="VK031_06825"
     CDS             complement(1305..1847)
                     /locus_tag="VK031_06825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HLS53668.1"
                     /translation="MAELISIRYAESLFEAGLELDKIDSFRKELESVQDLLRKEVEIF
                     EILKHPRITRKEKKSLIDEIFTGRLSQELINFFYVVIDRRREKYLHQIIEQFNQLYNQ
                     HMNIANVTAVTAIPMAEKPKERLASVLEERLGKKIVLSNEVDESIIGGVLLKIDNKFI
                     DSTLASQLKAMETAIKGVSL"
     gene            complement(1835..>2300)
                     /locus_tag="VK031_06830"
     CDS             complement(1835..>2300)
                     /locus_tag="VK031_06830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HLS53669.1"
                     /translation="TWVSVFILYLILRHFLYKPVSDFIEARQERIRTNIDEAKADKEE
                     AARLKEEYEEKLAASRAEGQEIIENARRRGVEVKEEIIEEARQEARAILDRATREIER
                     EKEKAYEDLRGSAGELAILIASRLMEEEINAEKQEQLIDKFIDEVGSAKWQS"
BASE COUNT          574 a          564 c          342 g          820 t
ORIGIN      
        1 aaactggact atattgaggt tgacgcaaga tctgcatcat tctttctcct tgatccagcc
       61 tctccctagt tccagcatcc agttcagatc caaactgggc aaatgctgcc agctccctat
      121 attgggccaa ctccaatttt aattggcctg ccactttttt aatggcctct atttgtgcag
      181 cccctccaac tctggataca gataatcctg tatttattgc cggtctttgc ccagcaaaga
      241 atagatcagt ttccaagaat atctgaccgt cagtaattga tattacgttt gtaggaatgt
      301 aggctgatat atccccagcc tgggtttcta caattggtag ggctgtaata gaacctcccc
      361 catatttgtc gtctaactta gccgcccttt ccagcaacct agaatgtaga taaaacacat
      421 ctcctgggaa tgcttccctt cctggtggcc ttcttaacag tagtgacatg gccctatagg
      481 ctgtggcgtg ctttgataaa tcatcatata taattaaaac gtctttgcct tgctccataa
      541 attcttcagc catagacact ccagcatagg gtgctatgta ctgaagtggt gccagctcac
      601 tggcagtaga tgatacaaca atggagtaat ccatagcccc atgtctttct aatacgtcta
      661 caatttgggc tactgtagat cttttttgtc ctatggccac gtagatacat attacatctt
      721 catccttctg attgattatg gtatctactg ccaaggtggt ctttccagtc tgcctatcac
      781 ctattataag ctccctttgc ccccttccaa ttgggaacat ggaatctata gccttaatcc
      841 ctgtttgtaa gggttcatgt acacttttcc ttgtaattac tcctggggct attctttcta
      901 tagggcggaa cctttcagcc cttatttctc ccttgccatc aattgggtgg cctagggcat
      961 ttactacccg tcctataagc tcttctccca caggtacttc aacgattctt tctgttcttt
     1021 ttactgtatc tccttctttt atattgtcat ctgaacctaa tagaacacat cctatattgt
     1081 cttcttccaa gtttaaggcc attccgaaaa cttctcctgg aaattctata agttctcctg
     1141 ccatacatcc ttccagtcca tgaattctgg caataccatc tccaacttgc atgaccgtac
     1201 ctatatctac cagatctagt cgtttttcat atttctttat ctgttctttc agtattgagc
     1261 taatttcttc cggtctcaac tccaaattca tcacctctat ttctctataa tgagacacct
     1321 ttaattgcag tttccatagc ctttaattga cttgctaatg tagagtcaat gaatttgttg
     1381 tcaattttta aaagaactcc acctattatg gattcatcaa cttcattgga aagtactatc
     1441 ttttttccaa gtctttcttc caatacacta gctaatcttt ccttaggttt ttctgccata
     1501 ggaattgcag ttacagcagt tacattggct atattcatat gctggttata taactggtta
     1561 aactgttcaa ttatttgatg caaatacttt tcccttctcc tatctattac tacataaaag
     1621 aagttaatta actcctggga taacctgccg gtaaaaatct catcaattaa agactttttt
     1681 tccttcctag taatcctggg atgtttcaat atttcaaata tttctacttc ttttcttaat
     1741 aagtcttgga cagattctaa ctctttacga aagctatcta tcttatccaa ctctagtcct
     1801 gcttcgaaaa gggattcagc atatctaata cttattaact ctgccatttc gcactaccta
     1861 cctcatcaat aaacttgtca attaattgct cttgtttctc agcattaatc tcttcttcca
     1921 ttaatctaga ggcaattaga attgctaatt ccccggcaga accccttaaa tcctcatatg
     1981 ctttctcttt ctccctttca atttcccttg tagctctatc taagattgca cgagcctctt
     2041 gtcgggcttc ctcaatgatt tcttctttaa cttctactcc acgacgccta gcattttcaa
     2101 taatttcttg tccctccgcc cttgatgcgg ccaatttttc ctcatattct tcttttaatc
     2161 ttgctgcttc ttctttgtca gctttagcct catctatatt ggtacggatc ctctcctgcc
     2221 tagcttctat aaagtctgaa accggcttat agaggaagtg tctaaggatt aaatataaaa
     2281 taaatacaga gacccatgta
//
LOCUS       DATGSI010000441         1736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_220697, whole genome shotgun sequence.
ACCESSION   DATGSI010000441 DATGSI010000000
VERSION     DATGSI010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220697"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VK031_06835"
     CDS             <1..656
                     /locus_tag="VK031_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HLS53670.1"
                     /translation="SGIYKILQDLVKGGFVIQSQSTKKYSVGPALYRLGYVYSERKGI
                     WDVANPVLKAISKITEETVSIGIREGDDAILAYKLEGPHIIRLEDRVGRKYPLNAGAI
                     GKLLAAYHNQDRIRKILSKSTLEKRTENTITDVKELLLEYEIIRSNGYAISNGENRAG
                     SYGISAPIYDRSGNVWSCLCLAGPKYRFTERKIKNWINLVIDGANEISYRLGYEGEY"
     gene            677..>1736
                     /gene="dcuC"
                     /locus_tag="VK031_06840"
     CDS             677..>1736
                     /gene="dcuC"
                     /locus_tag="VK031_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HLS53671.1"
                     /translation="MLGILIALVVTIVVGYLILKDYKPHSVLIFAGIILMLLSIVLNT
                     GSILPEDESTGFVLFDIFEFIKRSFSGHGANLGLIIMSVGGFARYMDHVGASRALVNI
                     AIKPLRRINAPYVVLAIGYIIGQILNIFIPSASGLGVLLMVTMYPILIELGVSSLSAT
                     AMIGTAACLDLGPASGASNLAAETSGLDATTYFMKYQVPVSIVVMIVIAILHYIVQKH
                     FDEKEGHNVVDARENNKESNEEVDVPGIYALLPIIPLILIFTFSKLIIDSISMNVITA
                     MLIGLILTMVCEMIRHRDIKSVFKSIEVFFNGMGAQWAAVITLIVAGETFAKGLQTIG
                     AIDTIIAGAQGASLGPALM"
BASE COUNT          601 a          241 c          378 g          516 t
ORIGIN      
        1 gaagtggtat ctataagatt ttgcaagatt tggttaaagg tggatttgtc atacaaagcc
       61 agtccacaaa gaaatattcc gtaggaccag ccctttatag gttaggctat gtatatagtg
      121 aaagaaaagg tatttgggat gtagctaacc ctgttttaaa ggcaatttct aaaatcactg
      181 aggaaaccgt ttccatcggt attcgagaag gggatgacgc aatacttgcc tacaaattag
      241 agggaccaca tattattagg ttggaagaca gagtaggaag gaagtatcct ttaaatgcag
      301 gggcaatagg taaactctta gccgcctatc ataaccaaga taggataaga aaaatactaa
      361 gcaaatcaac cctggaaaaa agaactgaaa atacaataac agatgttaaa gagttattat
      421 tagaatatga aataatacga tccaatggct atgctataag taatggtgag aatagagcag
      481 gttcatatgg aatatctgca cctatttatg acagaagtgg aaatgtatgg tcttgtttat
      541 gtttggccgg tcccaagtat cggtttacag aaaggaaaat taaaaactgg atcaatctgg
      601 ttattgacgg agcaaatgag atttcttata ggcttggata tgaaggagaa tattaaaatt
      661 aagaaggggg atttaaatgt taggaatatt aatagcttta gttgtaacta ttgtagtagg
      721 ttatttaata ttgaaggatt ataagccaca ctcagtacta atttttgccg gtattatatt
      781 gatgttatta tcaattgttt taaacactgg tagtatatta ccagaggatg aaagtacagg
      841 gtttgtacta tttgacattt ttgaatttat aaagaggtcc tttagtgggc atggagcaaa
      901 tctaggacta attataatgt ctgttggagg atttgccaga tatatggacc atgtaggggc
      961 aagtagggcc ttagtcaata ttgcaattaa accattaaga agaataaatg ctccatatgt
     1021 agttctggcc ataggctata ttataggaca aatattaaat atattcatac ctagtgcatc
     1081 tgggttagga gtactgctta tggtgaccat gtatccgata ctgatagaat taggggtcag
     1141 ctctttatca gctacagcaa tgataggtac tgcagcttgc ctagatttag gcccagcatc
     1201 tggtgcttca aacttggctg cggaaacatc aggacttgac gcaacaacct actttatgaa
     1261 atatcaagta ccagtatcca tagttgtaat gatagttatc gcaatacttc actacatagt
     1321 acaaaaacat tttgatgaaa aagagggaca taatgttgta gatgcaagag agaataataa
     1381 agaaagcaat gaagaagttg atgtacctgg tatatatgct ttactgccaa taatacctct
     1441 aatacttata ttcaccttta gtaaattaat aattgattca attagtatga atgtaattac
     1501 tgcaatgctt atagggctaa tattgacaat ggtttgtgaa atgattagac acagggatat
     1561 taagtctgta tttaaaagta tagaggtctt ctttaatgga atgggagctc aatgggccgc
     1621 agtaataacc ttaattgtag caggggagac ttttgcaaag ggactacaaa ctatcggagc
     1681 aatagataca ataatagcag gtgcacaggg ggcaagtctt gggcctgcat taatga
//
LOCUS       DATGSI010000442         1105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_220709, whole genome shotgun sequence.
ACCESSION   DATGSI010000442 DATGSI010000000
VERSION     DATGSI010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220709"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VK031_06845"
     CDS             <1..1029
                     /locus_tag="VK031_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HLS53672.1"
                     /translation="YIPSRVPFNFYTPDYEKGLELYSSGVLIMEKCPDLLPDHFSFVK
                     GVVDSEDLSLNISREMLQHDRQLKLIANRIESRIKDELKSLLENDREKYEEFYENFGQ
                     QLKYGVYDKYGANKDLLKDLLLFYSSKERKLVTLAEYVSRMPDDQKYIYYASGESIEQ
                     IDRLPQTELIKDRDYEILYFEDDIDEFAIRMLGEYEDKEFRSVSSGDLGLETDEETEA
                     IEKEMEESKGLFKEMKKVLKERVAKVKPSKRLKNHPVCLSSDGDISIEMEKVLKAMPN
                     NPDIKAEKILEININHQIYEKLKAAYENDKEKFKLYTKLLYDQALLIEGLNVEDPVEF
                     TNNICKLM"
BASE COUNT          439 a          131 c          217 g          318 t
ORIGIN      
        1 tatataccaa gcagagttcc ttttaatttt tatacaccgg attatgaaaa aggcttggag
       61 ctttattctt caggagtact tattatggaa aaatgtccag acctactacc agatcatttt
      121 agctttgtta agggagtagt agactctgaa gatttatcac ttaatatttc aagggaaatg
      181 ttgcagcatg atagacagct gaagctaata gccaatagaa ttgaaagcag aataaaggac
      241 gagctaaaat ctttgctgga aaatgatcga gaaaaatatg aggaatttta tgaaaacttt
      301 ggccagcaat taaagtatgg tgtttatgat aaatatggag caaataagga tttactaaaa
      361 gatttattat tattttactc ttccaaagaa aggaaattag ttacactagc tgagtacgta
      421 tctaggatgc cagatgatca aaaatatata tattatgcca gtggtgaatc tatagaacag
      481 atagataggt taccacaaac agaactgatc aaggacaggg actatgaaat cttatatttt
      541 gaagatgata tagatgagtt cgccataaga atgctgggag aatatgagga taaggagttt
      601 aggtctgtat ctagtggtga tctgggttta gaaacagatg aagaaactga ggctattgaa
      661 aaagaaatgg aagaaagcaa aggactcttt aaagagatga aaaaggtatt aaaggaaagg
      721 gtggcgaaag taaagccttc aaagaggctg aaaaatcatc cagtctgttt atcatctgat
      781 ggagatatat ctatagaaat ggaaaaagtg ctaaaagcta tgccaaataa tccagatatt
      841 aaggcagaaa agatacttga aataaatatt aaccaccaaa tatacgaaaa attgaaggca
      901 gcatatgaaa atgataaaga aaaatttaag ctttatacaa aattattata cgaccaagcc
      961 ctgctaatag agggattaaa tgtagaagat ccagttgaat ttactaataa tatctgtaaa
     1021 ctcatgtaaa ttttcataga aattcttgat atatttgtta aaaaactata taattgtctt
     1081 gtgataataa atattctttg taaag
//
LOCUS       DATGSI010000443         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_645663, whole genome shotgun sequence.
ACCESSION   DATGSI010000443 DATGSI010000000
VERSION     DATGSI010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645663"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            462..1211
                     /locus_tag="VK031_06850"
     CDS             462..1211
                     /locus_tag="VK031_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HLS53673.1"
                     /translation="MGKFQVEFTIPYFAGDKEGVLSPVFLLEYLGEVAALHSDAANLG
                     FKELKDMGYGWVLNRWKVKVDKYPKVKEKIIIKTWTSKAYKFFANREFTVYNKGGQLI
                     GKASTLWVFIDINRKRPARIPEKLVSSYKIVDKRNFKDFYKFQDDIGPVTGLDFLVRK
                     SDIDYNLHVNNVRYLDWILEVVPERENKEYFLKEFEIQYRQEIKYGEEVSSRINKGKR
                     VGKKKIYLHKITTDADEKSRAFAKTIWSKEK"
     gene            1235..1708
                     /locus_tag="VK031_06855"
     CDS             1235..1708
                     /locus_tag="VK031_06855"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004822157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HLS53674.1"
                     /translation="MNNTDWSLGSQKTIALVAHDNMKKDLINWVKRQKDLLAKHILCG
                     TGTTSKLISETTGLPVKSFKSGPLGGDQQIGARIAEGEIDFMIFFWDPLKAQPHDPDV
                     KALLRIAVLYDIPIANNKATADFLILSPLMDKTYDRHIVDFSELMEKRNKESFGM"
     gene            1987..2184
                     /locus_tag="VK031_06860"
     CDS             1987..2184
                     /locus_tag="VK031_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLS53675.1"
                     /translation="MKGTVKWFNAEKGYGFITTEEGNDVFAHFSQINKEGFKTLEEGQ
                     EVEFEVVEGQKGLQAENITIL"
     gene            2258..>2515
                     /locus_tag="VK031_06865"
     CDS             2258..>2515
                     /locus_tag="VK031_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLS53676.1"
                     /translation="MNKKVLAKVGGKEITQEDVFKFLREVGPQVAANFQSPEGMEKVV
                     EELVSQELLYLDAVENKLNEDEEYNRILEETKAMLLKNYAIS"
BASE COUNT          983 a          302 c          519 g          711 t
ORIGIN      
        1 aatgaaaatc cttattatat gagcatggca gtacaggggg ccatatcagc ctttgcatac
       61 tatattggaa aaaaagatag atttaaaaag atagagattc aggaggctat ggatctggca
      121 tataagattt tattaaagac attaaattaa aggggaacag ataaggaaaa ttaaaggttt
      181 taaaggacat cagtttccta ttatataaag ggtacagcat attaatgaat ataaatacca
      241 gtacatttta agatatatct gaaagaataa attaagaaat atgaaattga ataaaatagg
      301 cttaatccaa tatagttatc aaattttatt ggagatggaa taattacagg taagaaattt
      361 ataaaagtat taagtgggta ttaaaaacta aagtgtgggg taaaggctgt ctactaagat
      421 tacaggcttt accctgtaat ttcaaaggat aggtgaatga tgtgggaaaa tttcaagtag
      481 aatttactat accttacttt gctggtgaca aggaaggagt tttaagccct gttttcctat
      541 tagagtatct aggagaagtt gcagccttac acagtgatgc tgcaaattta ggttttaagg
      601 agctaaagga tatggggtat ggttgggtct taaatagatg gaaggtaaag gttgataaat
      661 atccaaaggt taaggaaaaa attattatta aaacttggac ctctaaggcc tataaatttt
      721 ttgccaatag agaatttacc gtatataata aaggtggaca gctaattggt aaggcctcta
      781 ccctatgggt ctttatagac attaatagaa aacgtccagc tagaatacct gaaaaactgg
      841 taagcagtta taagatagtt gacaaaagga actttaaaga cttttataaa tttcaggatg
      901 acataggacc tgtaacaggc ttagactttc tggtaaggaa aagtgatata gactataatc
      961 tacatgtaaa taatgttcgc tacctagatt ggatattgga agttgtgcct gaaagggaaa
     1021 ataaggaata ctttttaaaa gagtttgaaa ttcaatatag gcaagagatc aaatacggag
     1081 aggaagtctc ctcaagaata aataaaggca agagagttgg aaaaaagaaa atatatttac
     1141 ataaaataac cactgatgca gatgaaaaaa gcagggcatt tgcaaaaacc atttggagta
     1201 aggaaaaata agtgtaattt aaaggggaat tgatatgaat aatactgatt ggagcttagg
     1261 cagtcaaaaa accattgcct tggtggcgca tgataacatg aaaaaggacc taataaattg
     1321 ggtaaagaga caaaaggatt tactggctaa acacatccta tgtggaactg gaactacatc
     1381 taagcttatt tcagagacaa caggattacc ggtaaaatct tttaaatctg gcccattagg
     1441 tggagatcaa caaatagggg ctagaatagc agagggagaa atagacttta tgatattttt
     1501 ttgggatcca cttaaggcac aacctcatga tcctgatgta aaggccttac tcaggatagc
     1561 tgtattatat gatataccta tagctaataa taaggccaca gcagattttc tcattttatc
     1621 acctcttatg gataaaacct atgacaggca tattgtagat ttctctgagc taatggaaaa
     1681 gagaaataaa gaaagcttcg gtatgtagat aagggagctt atgtccaata agatgtcaag
     1741 atacaaattt aaaaatattt tacctaactt catttaaaaa gctattaact tttttaaaaa
     1801 gaaattaggt aaaaattact tgcaaaatat aggatatagt gatagtataa taattgagct
     1861 ttaattcaga agtaaaaatg caacacaaga atttctgcag tatcgagtat cacttgcatg
     1921 tatcagaaat tttatttgtg gtggttttac aaagaattaa agaaataaaa tttcggaggt
     1981 atatgcatga aaggtacagt aaaatggttc aatgcagaaa aaggttacgg atttataact
     2041 acagaagaag gaaatgatgt gtttgcacac ttttcccaaa tcaacaaaga aggcttcaaa
     2101 actttagaag aaggccaaga agttgaattt gaagtagttg aaggtcaaaa aggtctacaa
     2161 gctgagaata ttacaattct ttagtagata aaaattgcct cttggagact atgtctctag
     2221 gaggtttttt gttatcgaaa gaaaggaagt gttaagtttg aacaaaaagg tattagccaa
     2281 ggtgggagga aaagaaataa cccaggagga cgtatttaag tttttaagag aagtaggtcc
     2341 acaggtagca gcaaatttcc aatcacctga aggaatggaa aaagtagtag aggaattggt
     2401 ttcccaagaa ttactatatt tagatgcggt agaaaataag ctaaatgaag atgaggaata
     2461 taacaggatt ttagaagaaa ccaaggccat gttattaaaa aattatgcta taagc
//
LOCUS       DATGSI010000444         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_220725, whole genome shotgun sequence.
ACCESSION   DATGSI010000444 DATGSI010000000
VERSION     DATGSI010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220725"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VK031_06870"
     CDS             <1..184
                     /locus_tag="VK031_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HLS53677.1"
                     /translation="FAVCERENKEMIDIERDKLPKDAKEGDILIIEGKQIKVDKAATE
                     KRRKEIKKLMDSLWED"
     gene            281..577
                     /locus_tag="VK031_06875"
     CDS             281..577
                     /locus_tag="VK031_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HLS53678.1"
                     /translation="MLRQEYDMLKDHPGKSIFYFAIPMIIGNLFQKFYNMADSVIVGK
                     FVSEDALAAVGASYSLTTVFIMVAIGGGIGASVIISQYLGAKQYEEIKTSILVI"
     gene            652..843
                     /locus_tag="VK031_06880"
     CDS             652..843
                     /locus_tag="VK031_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53679.1"
                     /translation="MRVFTIANLINLSIRVTAAFKLAPVLGVAAVWYTIPMGWAANYM
                     VSFIYYLTGKWNVKSRYVI"
     gene            881..2524
                     /locus_tag="VK031_06885"
     CDS             881..2524
                     /locus_tag="VK031_06885"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HLS53680.1"
                     /translation="MQIQVKDILELEGFKDAKLVAGEKGLTNIVKNATLMEVPDIYRY
                     VEEDNLLITTLYPISSDREAMKELIPKLASLNLAGICIKPARYIKAIDSVMIKQANEL
                     NFPIISLPEGANLSDLVSEILEISLKNHIDMLHFRDLVHKELMDLFLSGADIGSMVDT
                     LANIVQLPVILLDNSYNITSKSKELKNRDIDILLEPNDYGRPGFKIKIDGRTYGVNRY
                     SRYSINAGKTRFGYLVLLKEKSESENVRIAMEQASLLLASAFYKNSAVMEKEKSFMDA
                     FIRELLQGKLASSMDAISKAKRFGWSMEFPMVIMVAKLLPKDNDRKFDLYGKILDSQL
                     LEKTLQERLNIKEDKINTVYIDNSIVIFINTIFMDDVRINCIKAGCSIMQKLKKETKI
                     GIGISNTINSFDGLPRAYEEAKRSIVAGTILYQESFTNHYNDYRIFDIINEVEDVSVL
                     ERFVDDKLGQVLEHDNSNDFQLLETLKVLIECNFNIKKASQELFIHYNTLRYRVDRLK
                     ELGINMDNGFELGELVLAFNIYLWLKGSQAKEKSLLRAD"
     gene            2752..>3553
                     /locus_tag="VK031_06890"
     CDS             2752..>3553
                     /locus_tag="VK031_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HLS53681.1"
                     /translation="MDVNVYKFSMAGPDDVSGLDELISSGQVKAEEIIAVLGKTEGNG
                     CVNDFTRGFCTASFRSYLAKKLNTSEEEVESKVAFVMSGGTEGVMTPHATIFTKSEVE
                     DAYSDDKSLAVSVGFTRDFLPEEMGTLTMVEEVKKVVLELMKEANIESKEDVHFVQIK
                     CPLLTSDRILEAKSRGKSVVVEDTYKSMGYTRGASALGVALALGEIEEGEVVEEAILN
                     DWSLYSEVASTSAGVELLNCEIIIMGNSHKSVSKYRIGHSVMEDAIDME"
BASE COUNT         1230 a          447 c          792 g         1084 t
ORIGIN      
        1 atttgcagta tgtgaaagag aaaataaaga gatgattgat atagaaaggg ataagttacc
       61 aaaagatgct aaagaaggtg acatcctgat tattgaaggg aaacaaatta aagtagataa
      121 ggctgccaca gaaaaaagaa gaaaagaaat taagaaactt atggatagtt tatgggaaga
      181 ctaggatagg cctagtcttt cttctatcct tgattaatat atagaaatat atgataatta
      241 atattatcaa acaagattgt gacagaaaga ggaatacata atgttaagac aagaatatga
      301 tatgttgaag gatcatccag gaaaatccat attttatttt gcaattccta tgattatagg
      361 taacctattt caaaaatttt acaacatggc tgactcggtt attgtaggaa aatttgtaag
      421 tgaagatgcc ttggcagcag tgggggcatc ttattctttg actactgtat ttataatggt
      481 tgccataggt ggcggaattg gggcatcagt tattatatcc cagtatttag gggcaaaaca
      541 atatgaggag ataaaaactt caattttagt tatttaagat ttattggttt tttctttgta
      601 tttataggat taaaatctat tactgatggg ttgcttcggg gaattggaga tatgagagta
      661 tttaccatag ccaacttaat taatcttagt attagggtaa cagctgcttt taaattggca
      721 ccagtcctag gggtggctgc tgtatggtat acaattccta tgggttgggc tgccaattat
      781 atggtttcct ttatatatta tttaactgga aaatggaatg ttaaaagtag atatgtgata
      841 taatgacagg ggaaattttt ataagaaatg agtgaagctt atgcaaatac aggtcaaaga
      901 tatattggaa ttggaaggct tcaaggatgc taagctagta gcaggagaga agggattaac
      961 taatatcgtt aaaaatgcta ccttaatgga ggtaccagat atatatagat atgtggaaga
     1021 ggacaatttg cttattacaa ccttataccc aatatctagc gatagggagg caatgaagga
     1081 gctgattccc aagctggcta gcttaaattt ggctgggatt tgtataaaac cggccagata
     1141 tattaaggca attgattctg taatgattaa acaggccaat gaactaaatt ttccaattat
     1201 tagcctacct gaaggagcta atttatcaga tttggtatca gaaatcctag aaatatctct
     1261 taagaaccac atagatatgc tccattttag agaccttgtt cacaaggagt tgatggatct
     1321 attcctaagt ggggcggata taggatccat ggtagatact ttggctaata tagttcaatt
     1381 gcctgtgatt ttattggaca actcctataa tattacttca aaatctaagg agttaaaaaa
     1441 tagggatata gatatacttt tagagcctaa cgattatggc agaccaggat ttaaaataaa
     1501 aatagacgga aggacttatg gagtcaatcg ttattctaga tatagtatta atgcaggtaa
     1561 aacaagattt ggttatctgg tccttttaaa agagaaaagt gaaagtgaaa atgtaaggat
     1621 tgccatggag caggccagcc tacttttggc ctctgccttt tataaaaata gtgcagttat
     1681 ggaaaaggag aaaagtttta tggatgcctt tatcagggaa ttactgcaag ggaaacttgc
     1741 ctcttccatg gacgccataa gcaaggctaa aagatttggt tggagtatgg aatttcctat
     1801 ggttattatg gtggcaaaat tgctgccaaa ggataatgat agaaaatttg atctgtatgg
     1861 taaaatttta gattcacaac ttttggaaaa gactctacag gaaagactga atataaaaga
     1921 ggataaaata aacactgtct atattgacaa ctctatagta atctttatta atacaatttt
     1981 tatggatgat gtaaggatta attgcatcaa agcaggctgt tccattatgc aaaaattaaa
     2041 gaaagagact aaaataggca taggcatatc taatactatt aactcctttg atggcttacc
     2101 aagggcctat gaagaggcta aaagaagtat tgttgctgga actattttgt atcaggagtc
     2161 ttttacaaat cattacaatg attacaggat ttttgacatt attaatgaag tagaagatgt
     2221 tagtgtctta gaaagatttg tagatgataa attaggccag gttttagagc atgataattc
     2281 caatgatttt caattgttgg agaccttgaa agtattgatt gaatgcaatt ttaatataaa
     2341 gaaagcctcc caggaattat ttattcacta taatacctta agatataggg tggacaggct
     2401 taaagaatta gggattaata tggataatgg ttttgagcta ggggagttgg ttcttgcctt
     2461 taacatatat ttatggttaa aaggtagcca ggctaaggaa aaaagtctat tacgggctga
     2521 ttaaggatag gtattttatc ccattacaag tgaagtttcc aattgaatag atgagaagaa
     2581 cggatatcct tgtcaataag tgacaaggat atccattttt tttggcttta ggttatgatg
     2641 attaaccaag accccaatgc tataatcaag tcacaactgg aaaaagtttt cataaaaaac
     2701 ttaataattg tcaaaactaa taaggaatag aaaaatttag gaggtagtgt tatggatgta
     2761 aatgtatata agttttcaat ggctggacct gatgatgttt caggattaga tgagttaatc
     2821 tccagtggcc aagtaaaggc agaggaaatt atagctgtgt taggtaaaac tgagggcaat
     2881 ggttgtgtaa atgatttcac aagaggattt tgcacagcat cctttagaag ttatttagct
     2941 aagaagttaa ataccagtga agaggaagta gaatctaagg tggcctttgt catgtctggt
     3001 ggaactgagg gagttatgac acctcatgct accatcttta caaaatcaga ggtggaagat
     3061 gcttattctg acgataagtc cttggctgta agcgtaggct ttactagaga ttttttacca
     3121 gaagaaatgg gaactctaac tatggtggag gaagtaaaaa aggtagtatt ggagctgatg
     3181 aaggaagcaa atattgaaag taaagaggat gttcactttg ttcagattaa atgccctctt
     3241 ttaacctcag acaggatcct ggaggccaaa tccaggggta agtcagtggt ggtagaggat
     3301 acttataagt ctatgggtta tacaagaggg gcttctgccc tgggagtggc cttagcccta
     3361 ggggagatag aagaagggga agtggttgaa gaggctattt taaatgactg gtccctgtat
     3421 tcagaagtag cttcaacctc agcaggagtt gaattgctaa attgtgaaat tatcataatg
     3481 ggtaattctc ataagtctgt aagtaagtat aggataggcc atagtgtaat ggaagatgca
     3541 atagatatgg aag
//
LOCUS       DATGSI010000445         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_599531, whole genome shotgun sequence.
ACCESSION   DATGSI010000445 DATGSI010000000
VERSION     DATGSI010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599531"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VK031_06895"
     CDS             complement(<1..317)
                     /locus_tag="VK031_06895"
                     /inference="COORDINATES: protein motif:HMM:NF018240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrpR N-terminal domain-containing protein"
                     /protein_id="HLS53682.1"
                     /translation="MKRIKALGIAPYEGVKEIMDKIALEKSELDLTTYTANLEDAIDI
                     IKNSKENYEVIISRGGTAKLLEDYVDIPIVEISFTVYDILRSIDEAKRYTNSYSILGY
                     HNL"
     gene            558..1802
                     /locus_tag="VK031_06900"
     CDS             558..1802
                     /locus_tag="VK031_06900"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HLS53683.1"
                     /translation="MEIKLLVIADDLTGALDTGVKLAKEKISTLIVLNFKEIEKQDLK
                     DYEVIVIDTNSRHMEAKKAGQKIEDILRHFKDLEVDIEYIYKKTDSALRGNIGSELSA
                     LLRASDSKELHFIPALPEFNRVTIDGIHYIDGKKLEDSVFAKDPFEPVEYSSVKDIIK
                     SQSNIETITTNTYINESKNERIIIYDAKVEQDILDIAKSLFKKGRLKTLAGCAGFINV
                     LPELIEFNKTSRVKNQVLGNFLAVCGSINPVTQRQVEYALDNGFKGILLKVKDLETRK
                     DYWSTSTGKAIIEEITKSLHSQEKVIVYLDTLFGNKKNLNMDGRFKIAEALGQLVQII
                     VTNNIAKNVLITGGDTLRGLCNVANIKRLIPHHELMEGIVKAKVEINNTKFNLISKSG
                     GFGDEKTISEIAGILCAKESCI"
     gene            1799..>2205
                     /locus_tag="VK031_06905"
     CDS             1799..>2205
                     /locus_tag="VK031_06905"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HLS53684.1"
                     /translation="MKTAIVNFPGQVIYGRESIKKLKDKVKGHSNVALFAYGSAGKNK
                     GYQLILEILDGEGISYTEIREIPAEPSYLEVQEIYDRLDENVDLIIAFGGGSVIDTAK
                     LISIMDRDLSVKDLIDDSSKGNKKIKTITIPTT"
BASE COUNT          832 a          293 c          419 g          661 t
ORIGIN      
        1 taaagattat gataccctaa tatagagtaa gaattggtat atcttttggc ttcatcaatt
       61 gagcgaagaa tgtcatatac agtaaaagat atttctacaa taggtatatc aacatagtct
      121 tccagtaatt ttgcagtacc tcctctagaa ataattacct catagttttc ttttgaattt
      181 tttataatat caatagcatc ctccaggtta gcagtatatg tagtcagatc taattcactt
      241 ttctctaagg ctatcttatc cataatttct ttaactcctt catagggagc tattcctaaa
      301 gctttgattc ttttcaatac attcaccgtc ctctaattta ttgacaccta taaagataag
      361 tgattttgga acaatttact ctatattata tattataatg gataaatatg aaacataatt
      421 ggaggattat acaaaatatg taccaatata aaacacaaat tattgtgaaa gattaaacgc
      481 ttcatattga cacaaaaaca ttttcctagg ttttgcaaat gatttcataa cttggtaacc
      541 tttatctgaa gggagtggtg gaaataaagt tactggttat agctgatgat ctaacaggag
      601 ctctagatac aggggtaaag ttagccaaag aaaaaatatc aacactaata gtattaaatt
      661 tcaaagagat agaaaagcaa gacttaaagg actatgaagt tatagttatt gatactaatt
      721 ctcgacatat ggaggcaaag aaagccggtc aaaagataga agatatatta aggcacttta
      781 aagacttaga ggtagatata gaatatatat acaaaaaaac cgactctgct ctaagaggaa
      841 atataggaag tgaattaagt gccctattaa gagcatctga ttccaaagaa ttacatttca
      901 taccggcctt acctgaattt aatagggtca ctattgatgg gattcattat atagatggaa
      961 agaagttgga ggattcagta tttgccaagg atccttttga accagttgag tattcttcag
     1021 tcaaagatat aattaaaagc caaagcaata ttgaaaccat tactactaat acttatataa
     1081 atgaaagtaa gaatgagaga attataatat atgatgcaaa agttgaacaa gacatattag
     1141 atatagccaa atctcttttt aaaaaaggga gattgaaaac tttagcaggt tgtgcaggtt
     1201 ttataaatgt cttacctgag cttattgagt ttaataagac aagtagggta aaaaaccagg
     1261 tattgggaaa cttcttagca gtttgtggta gtataaatcc tgtaactcaa agacaggtag
     1321 agtatgcact tgataatggc tttaagggta tactgcttaa ggtgaaggac ttggaaacaa
     1381 gaaaagatta ttggtcaact agcacaggca aagccataat tgaagaaatt actaagtcct
     1441 tacatagcca ggagaaggta attgtatatt tggataccct atttggcaat aagaagaatt
     1501 taaatatgga tggaagattt aagattgccg aagctttagg acaattggtt caaataattg
     1561 taacaaacaa tattgccaaa aatgttttga taacaggcgg ggatacccta agaggactat
     1621 gtaatgtggc taatataaaa agattaatac ctcaccatga gcttatggaa gggatagtaa
     1681 aagctaaggt ggagatcaat aatacaaaat ttaatctaat atcaaaatct ggtggttttg
     1741 gtgatgagaa aaccattagt gagatagcag ggatattatg tgcaaaggag tcgtgtatat
     1801 gaagactgca attgtaaact ttccaggaca agtaatatac ggaagagaat ctattaaaaa
     1861 attaaaagat aaggtaaaag gtcattccaa tgtggcccta tttgcctacg gaagtgctgg
     1921 caaaaacaag ggctaccaac ttattttgga aatcttagat ggggaaggaa tttcttatac
     1981 ggaaattagg gaaatacctg ctgaacccag ttacctagaa gtgcaagaaa tatatgatag
     2041 gctagatgaa aatgtagatt taataattgc ctttggtgga ggtagcgtaa tagatactgc
     2101 taagctgata agtataatgg atagggatct ttctgttaag gatttaattg atgatagttc
     2161 aaagggtaat aagaagataa aaaccataac aattcctaca acatg
//
LOCUS       DATGSI010000446         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_29212, whole genome shotgun sequence.
ACCESSION   DATGSI010000446 DATGSI010000000
VERSION     DATGSI010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29212"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..802)
                     /locus_tag="VK031_06910"
     CDS             complement(365..802)
                     /locus_tag="VK031_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLS53685.1"
                     /translation="MKENKKNYYIFLEDTKVDVSEKVYKGYWQETNRENYLNRLDKEN
                     KLGCFSEFVLDETDKSMEERLVDKAVDVEKLVAVKMQIEALNRALDKLSPEEREIIQA
                     LFFDEVPQRDLAKKLNISQGAVFRRKEKTLKKLKIFLEDWKEK"
     gene            complement(1076..1636)
                     /locus_tag="VK031_06915"
     CDS             complement(1076..1636)
                     /locus_tag="VK031_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53686.1"
                     /translation="MNFNGMIKEYKLENLDKKKLRNDIKLLLSLYRKVDFHIQDHIEN
                     LDNIFYETKRKHLDDIVLSILEIDKDINIEKVESQLLNTQQSIFLLKLMDDALSRLSA
                     YPNKGEIYADIITYKYFEKKIRSNEEISELLLISRSTYYRYMNKAILIFGVMLFGYSV
                     FDRNYEDLSNCKLKVAEDKIDYKIEL"
     gene            complement(1753..2070)
                     /locus_tag="VK031_06920"
     CDS             complement(1753..2070)
                     /locus_tag="VK031_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53687.1"
                     /translation="MEEDKFREKKIKYGPGNCEDIIGIKGYKAKAQIISYLADDSRGI
                     LPHGIVNRSIIFVDTKASYKEGDLIIIKDLRNKKDGVRIAKAISKKEKDFMGRIFLVT
                     TTY"
     gene            complement(2296..>2747)
                     /locus_tag="VK031_06925"
     CDS             complement(2296..>2747)
                     /locus_tag="VK031_06925"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HLS53688.1"
                     /translation="IAREDFKEKIWNLPVEELLGVGKSSKKKLNRLMIFTIGDLARGD
                     PDLIKMKLGINGVYLWNYANGRDYSSVTDRDYRDPVKSVGRGITTTEDLENNLEVYRV
                     FRELSFGVSKALRSYGYLAGGIQISVKNNNLLSKQYQRQISYQSFNY"
BASE COUNT          791 a          562 c          304 g         1090 t
ORIGIN      
        1 aatttcacct ctcccctctt ctcggtaggg aacaacaacg tatagaagta tcattatcac
       61 catttatatc tttgcaagaa aggttgccaa tcatttcttg tagcaaacta tttatcgctt
      121 acatttttct atcctttgca tttagcgtta tgcaagtatt tatttaaacc aaggcttggc
      181 cttagcaaaa tggtcatggc gtagtttcta ctggcttcat aaatggataa agtcttgtcc
      241 tggtctattt agttttcaaa gttcaaaata tataaatttt tattgttata taaaggactt
      301 cattttgtcc cttatatata acagtgaaaa aaccaccccc ttgattcgct tttttaaaaa
      361 ctttttattt ttctttccaa tcttctaaaa agatttttaa tttttttaag gttttttctt
      421 ttcttctaaa tactgctcct tggctaatat ttagtttttt tgctaaatct ctttggggaa
      481 cttcatcaaa aaaaagagcc tgaataattt ccctttcttc tgggcttaat ttatccaagg
      541 ctctattaag tgcttctatt tgcattttta ctgctactaa tttttctaca tcaactgctt
      601 tatctacaag tctctcctcc atgctcttgt ctgtttcatc taatacaaat tcagagaaac
      661 aacctaattt attctctttg tctagcctat taagataatt ctctctattg gtttcttgcc
      721 aatatccttt atatactttt tctgatacat ccaccttggt atcttcaagg aatatataat
      781 agttcttttt attttctttc attttctttc ctcctgtgaa tttaaaattt tcaatttaaa
      841 attcacaaag aggaggttct ctaatgtatt tcacctgttt cacctcaatt tagggcaaaa
      901 aaagacaagt gtaagagcca cttgtcttct tcaaacctta tatttaggca taaaaaaacc
      961 tccatttttc caaacagagg ttgcctatta tattgagtgt aacaggctta aagtctcatt
     1021 acagtaccaa aagggttcca gttttatccc attttagtcc cataaaagtc tcattttata
     1081 attcaatttt ataatctatc ttatcttctg ctacttttag cttacaattt gataagtctt
     1141 cataatttct atcgaaaaca ctatatccaa aaagcatgac tccaaatatg agtatagctt
     1201 tattcatata tctataatag gtggatcttg aaattaaaag caattcagaa atttcctcat
     1261 tacttcttat ttttttctca aaatatttat aagtaataat atctgcataa atttcaccct
     1321 tgtttggata agcacttaat ctagataaag catcatccat aagcttaagt aaaaatatgc
     1381 tttgttgtgt atttaagagc tggctttcaa ccttctctat gttaatatcc ttatctattt
     1441 ctaatataga taggacaata tcatccaaat gttttctttt tgtttcgtaa aatatattat
     1501 ctagattttc tatatggtcc tggatgtgga aatctacttt tcgatacaaa ctcaggagaa
     1561 gtttaatatc attccttaac ttctttttat ctagattttc taatttgtat tcctttatca
     1621 tcccattaaa attcatgata taccctcctc ctaacgaaaa taaagtttct tttgcgtcct
     1681 agaccaaagt cccagaatac ccattccgta atatcccagg attttgcatt aacttgcatt
     1741 tttactccct ccttaatatg ttgttgtaac taggaatatt cttcccataa agtccttttc
     1801 ttttttacta attgcttttg ctatccttac tccatccttc ttatttctca aatccttaat
     1861 tataattaaa tctccctctt tataactagc cttggtatct acaaaaatta tacttctatt
     1921 gactatacca tgtggtaaaa tccccctact atcatcagct aggtaggaga taatctgtgc
     1981 ttttgcctta tatcctttta ttcctataat gtcctcacaa tttcctggac catacttgat
     2041 tttcttttct ctaaacttat cctcttccat cttgtctccc cctatctagt tccaccaggt
     2101 agagttacta tttcatttct ctccataggc atcttaatat ctcccataag accagcataa
     2161 gaaatagaat agtcaccaaa tctctctctg attttaaaga aggcatcatc taccttctct
     2221 ctctttatta gttttgaata gtcctcatag aagcttaatt gacttccatt cttctctcct
     2281 attagattta ttgctctaat agttaaagct ctgataggat atctgtcttt gatattgctt
     2341 agataataga ttgttatttt taacggatat ttgaattcct ccagctaagt atccatagct
     2401 tcttaaggcc ttggatactc caaaggataa ttctctaaat accctataaa cttctaagtt
     2461 gttttctaaa tcttctgtag tagttattcc tctccctacc gacttaactg gatccctata
     2521 gtccctatca gttactgaac tataatctct accattggca tagttccata aataaactcc
     2581 attaatgcct aacttcatct taattaaatc tggatcccct ctagccaagt ctcctatagt
     2641 gaaaatcatt agcctattta actttttctt agaagactta cctactccta aaagttcttc
     2701 tactggtaaa ttccaaatct tctccttaaa atcttcccta gctattt
//
LOCUS       DATGSI010000447         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_173969, whole genome shotgun sequence.
ACCESSION   DATGSI010000447 DATGSI010000000
VERSION     DATGSI010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173969"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VK031_06930"
     CDS             complement(<1..155)
                     /locus_tag="VK031_06930"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HLS53689.1"
                     /translation="MRKRNFKQKEYLYVDGYNIINSWDSLKTKKRYSLEEARIELLEI
                     LSEYHHYS"
     gene            complement(136..885)
                     /gene="rlmB"
                     /locus_tag="VK031_06935"
     CDS             complement(136..885)
                     /gene="rlmB"
                     /locus_tag="VK031_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HLS53690.1"
                     /translation="MKNLHIIGRNPVLELLKANRKINRLYILRGDLRGSINEIIKLAE
                     KKNIDIERVDRKRLDSIAAGGVHQGVAALIPEYKYATVDEILNKAKSMSQDPFILILD
                     QIEDPHNLGAIIRTAECAGVHGIIIPKRRSAHVNQTVYSASAGAAEHMLVADVVNITN
                     TIRELKEKGLWIYGADVEGEQYHFNTSLKGPVALVIGNEGRGISRLVKENCDVLVKIP
                     MYGKISSLNASTSAAILIYEVVRQNHEKEEF"
     gene            complement(941..1699)
                     /gene="thyX"
                     /locus_tag="VK031_06940"
     CDS             complement(941..1699)
                     /gene="thyX"
                     /locus_tag="VK031_06940"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HLS53691.1"
                     /translation="MKVELLRYTPDGEKLIASAARLCYSPVGISQIEENLKEERVDSF
                     LQMLVDLGHESPIEHVSFTFGVEGVSRSLTHQLVRHRIGSYSQQSQRYVKLDQFEYII
                     PPAIAQSKEAKALFVKAMEEDQKYYDAITEILFKKHYDRLIGEGKKEKEARRAAEKEA
                     IEDARYIFPNACETKIVFTMNARSLLNFFRLRCCNRAQWEIRELAINMLKLVKKVYPT
                     LFKNAGPGCLKGPCPEGSMTCGKIKEVREKFRKL"
     gene            complement(1745..2167)
                     /locus_tag="VK031_06945"
     CDS             complement(1745..2167)
                     /locus_tag="VK031_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HLS53692.1"
                     /translation="MSKKNIFRNINESLTEEDVKMLQPLQLAYVGDAVYELLVRTFLL
                     DGNQAVNQLHKKATRLVKAQSQASIVHKLEDYLTDKEKTIVRQGRNAKSSGGSKNVDL
                     INYKYATGFEALMGYLYLTGQEERLSSLFEKIISLKID"
     gene            complement(2169..>2858)
                     /locus_tag="VK031_06950"
     CDS             complement(2169..>2858)
                     /locus_tag="VK031_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HLS53693.1"
                     /translation="EIAQSETLTGKPFANYWLHNGMITVDNRKMSKSEGNFFTIKDVT
                     KEYDLETLRFFLLSAHYRTPINFSREVMNHTKNGLERLYNGKHRLEDLLDMAEERPLN
                     TEDEELIDSINGFKDKFVSAMEDDLNTADAIASLFDLIRHTNSKLNEKSNRLVVEKAH
                     ESLEELADILGILKREEEVLKEDILNLIEKRTEARKNKDYKLADQIRDQLLEKGIMLE
                     DTKDGVKWKRV"
BASE COUNT          755 a          651 c          403 g         1049 t
ORIGIN      
        1 gaataatggt gatactcaga taggatttcc aacagttcta ttcgagcctc ctccaggcta
       61 tatctttttt tagtcttcag gctatcccat gagtttatta tattgtatcc atctacgtat
      121 aaatattctt tttgtttaaa attcctcttt ctcatgattc tgtctcacaa cctcataaat
      181 caatattgca gctgaagtag aagcattaag agaggagatt ttcccataca taggtatctt
      241 aactaataca tcacaatttt cctttaccaa tcttgatata cctcttcctt cattccctat
      301 taccaaggcc acagggccct ttagggaggt attaaaatga tattgctcac cttccacatc
      361 agccccgtaa atccataatc ccttttcctt aagctccctt atggtatttg ttatgttaac
      421 tacatcagcc accaacatat gctctgcagc tcctgctgag gcgctgtaga ctgtctggtt
      481 aacatgagct gatcgtcttt taggaataat aattccatgt accccagcac attcagctgt
      541 cctaattata gcacctagat tatgaggatc ctctatttgg tctaagatta agatgaaggg
      601 atcttggctc atagattttg ccttgtttaa aatttcatct actgtagcgt atttgtattc
      661 aggaattagg gcagcaacac cctggtggac tccaccagcg gctattgaat ctagtctttt
      721 tctgtctacc ctttcaatat ctatattctt tttttcagct aacttaataa tttcatttat
      781 ggagcccctt agatctcctc ttagtatata aagcctattt atctttctat tagcctttaa
      841 caattctaaa actgggtttc tccctataat gtgtaaattc ttcatccatc tcaatccctt
      901 caaattactt agtccctatt tagttccaat aaatttaatt ttaaagcttt ctaaattttt
      961 ctcttacttc tttaatcttc ccacaagtca tgcttccctc tgggcaaggc ccctttaaac
     1021 agccaggacc tgcattttta aacagggttg ggtatacttt ttttaccagt ttaagcatat
     1081 ttatagccaa ttctctaatt tcccattggg ccctattaca acatctgagc ctgaaaaagt
     1141 taagtagtga tctggcattc atagtaaaaa ctatcttagt ttcacaagca ttagggaaaa
     1201 tatatctggc atcttcgatg gcttcttttt ctgccgccct tcttgcctct ttttcctttt
     1261 ttccctctcc aattagtcta tcataatgtt tcttaaataa aatttctgtg attgcatcat
     1321 aatatttttg gtcttcttcc atggccttaa caaacaaggc tttggcctcc ttgctttggg
     1381 ctatggccgg tggaataatg tattcaaatt gatccaactt aacatatctt tgagattgtt
     1441 gggagtaact accaatccta tgccttacca actgatgggt taaggacctg gacactcctt
     1501 ccactccaaa agtaaagctt acatgctcaa tagggctttc gtggcctaag tccaccaaca
     1561 tttgtaaaaa ggagtcaacc cgttcttcct ttaaattttc ttctatttgg ctgattccta
     1621 ctggtgaata acaaagcctg gctgcagaag caattagttt ttctccatct ggcgtatatc
     1681 ttaaaagttc aactttcaat ttagtgtccc ccttagttat gtattactat aattatattg
     1741 tactttagtc aattttcaag cttataattt tttcaaatag actactaagc ctctcctcct
     1801 gccctgtaag atacaggtat cccattaggg cttcaaatcc tgtagcatat ttatagttga
     1861 ttaaatccac atttttagat cctccactgg acttggcatt tctcccttgt cttacaatag
     1921 ttttttcttt gtcagtcaaa tagtcttcca acttatgaac tatactggcc tgagactgtg
     1981 ccttgaccag ccttgtggcc ttcttatgca actgatttac agcctgattc ccatctagaa
     2041 gaaaggttct tactaataat tcataaactg catctcccac ataggccaac tgaagaggct
     2101 gtagcatttt tacatcctct tcagtcaggc tctcatttat attcctaaat atatttttct
     2161 tactcatcct acactctctt ccattttact ccatccttag tatcttccaa cataattcct
     2221 ttttccagaa gctggtcccg gatctgatca gccaatttat aatctttgtt ttttcttgcc
     2281 tctgttctct tctcaattag atttaatata tcttctttaa gaacctcttc ctccctcttt
     2341 aaaataccta atatatctgc caactcctct aggctttcat gagccttttc aactaccaat
     2401 ctattggact tttcatttaa tttactgttt gtatgtctaa ttaggtcaaa taaagaagca
     2461 atggcatctg ctgtatttaa atcatcttcc atggcagata caaatttgtc cttaaaacca
     2521 ttaattgaat ctattaactc ctcatcctct gtatttaaag gcctttcctc agccatatct
     2581 aatagatcct ccagcctatg cttgccatta tacagtcttt caagcccatt ttttgtatga
     2641 ttcataacct cccggctaaa gttaatagga gtcctatagt gggctgatag gaggaaaaat
     2701 cttagagttt ctaaatcata ctccttggtt acatccttaa ttgtgaagaa atttccttct
     2761 gactttgaca tttttctatt atccacagtt atcatcccat tatgcaacca gtaattggca
     2821 aagggctttc ctgttagagt ctcagactgg gctatttc
//
LOCUS       DATGSI010000448         1575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_262757, whole genome shotgun sequence.
ACCESSION   DATGSI010000448 DATGSI010000000
VERSION     DATGSI010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262757"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..>1575)
                     /locus_tag="VK031_06955"
     CDS             complement(220..>1575)
                     /locus_tag="VK031_06955"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HLS53694.1"
                     /translation="SFALDTTGAIILLIAIYYGSLFGGMITAILLNVPGEATSAITCF
                     DGHPLAKEGRAGPALFIAAISSATGGLISTVALVVAAPVVAGFAFKFGPPEQLALLIF
                     ALSLLFILSGVSMIKTLMMGITGLALGTVGLDPTQAYPRFTFGIPELFEGISFVPIVM
                     GLFGLADILYQLEEKAKKAEILKIGKLMPSKADWKAVRGTIVRGSGIGFFLGLIPGMM
                     PSISSFLSYSTEKSIAKKPERFGKGAVEGIAGPEAANNAHVQATLIPLLTMGIPTTPT
                     IALILGAFLVNGIVPGPLLFRDNPTLVWTVIASFFIGNIFLLVWNLPLIKMWVSILKT
                     PSSILYSVIIVLMIVGSYSEYNGMFGVSLLLGAGVFGYWMKKLDFPVAPLILTLVLGP
                     MLEESLIRTLEMFPTGGAIEIFKRPIVLSLAILTISVFVVLIYKSITKLSSGQSLASS
                     S"
BASE COUNT          535 a          330 c          296 g          414 t
ORIGIN      
        1 actagctgcg ggatccacta cacatagaac tgcagaagca gtaatagctg catccattgc
       61 attgccccct ctttgcaaga tagttaatgc agcctggctg gctagcgggt gtcccgtggc
      121 taccattcca ttggcagcat aaataatcgg acgagtaaga tgagagtcat atgtcatgat
      181 tttatatttc ctgaaattat gagtgttacg aggttacgtt taactggacg aggccagaga
      241 ctgacctgat gaaagtttag ttattgattt atagattagg actacaaaaa ctgatatcgt
      301 aagaatggct aaacttaaaa ctataggacg cttaaatatt tctatggctc cacctgttgg
      361 aaacatctct aaagttctta ttagcgactc ttctagcatg ggacctagga ctagggtaag
      421 tattaatggg gctacaggaa aatctaattt tttcatccag taaccaaaaa cacctgcccc
      481 taataataaa ctcacgccaa acattccgtt atactctgaa taagaaccaa ctatcattaa
      541 aactataatg acggagtata aaattgaaga cggtgttttt aagatagaaa cccacatttt
      601 tattaaggga agattccaaa ctaacaaaaa tatatttcca ataaaaaaac tagctatgac
      661 agtccaaacc aaggtagggt tatccctaaa caagagaggc cctgggacaa taccatttac
      721 tagaaaagcc ccaagaatta aggcaatagt aggagtggta ggaattccca tggttagcaa
      781 aggtatcaaa gtagcttgca cgtgtgcatt atttgctgct tccggacctg caattccctc
      841 cacagcgcct tttccaaaac gttcaggttt cttagcaata ctcttttctg tagaatatga
      901 aaggaatgaa gaaatagagg gcatcatccc tggtatcagg cctagaaaaa aaccaatgcc
      961 tgatccacga actattgttc ctcttacagc tttccaatcg gcttttgagg gcataagctt
     1021 acctatcttt aatatttctg cttttttagc cttttcttct aattgatata aaatatctgc
     1081 taagccaaac aatcccatca cgataggcac aaaggaaatc ccttcaaata gttcaggaat
     1141 gccaaaagta aaacgagggt aagcctgagt agggtctagc ccaacagtac ctaaagctaa
     1201 acctgtgatt cccatcatta aggttttaat catcgatact cctgacaaga taaagagtag
     1261 tgacaaagca aaaatcaata acgctaactg ctcagggggg ccaaacttaa aagcaaatcc
     1321 tgctacaaca ggtgccgcca caaccaaagc cacagttgat attaagcccc cagtagcgga
     1381 agaaatagca gctataaaaa gagcaggacc agctctacct tctttagcta atgggtgtcc
     1441 atcaaaacat gttatggcac tggttgcttc gcctggtaca ttaagcaaaa tggcggtgat
     1501 catacctcca aataaactcc cataatagat agcaattaat aaaataattg cacctgttgt
     1561 atctaaagca aaaga
//
LOCUS       DATGSI010000449         1099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_174213, whole genome shotgun sequence.
ACCESSION   DATGSI010000449 DATGSI010000000
VERSION     DATGSI010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174213"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VK031_06960"
     CDS             complement(<1..1008)
                     /locus_tag="VK031_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLS53695.1"
                     /translation="MIDKELINLRRYFHQNAEPGWMEFKTTDKIISELKDLDLKLFYG
                     KEIYFNKRLGLYDSKNLRLYKDKISLDNEDKKDEILDSFTGAIALIDSKRPGPNIGFR
                     FDIDANDLKESDSKGHIPNIKNFASKNDFAMHACGHDAHTAIGISLCKYFAQNKDKFR
                     GKILVIFQPAEEGVRGAYSLMGNPMIDKLDYMAAMHIGMDLPSGFISVGSGGFLATKK
                     LDISFKGRTSHAGASPEKGHNALLAASSAILNFNNLSQNSNGVSRINVGVLKAGSGRN
                     IIPNKANLKMEIRGENQKIIDYLYQGVLRIVEGAALSFECSFDINLVGSAPALLDYDE
                     DF"
BASE COUNT          328 a          227 c          145 g          399 t
ORIGIN      
        1 aaaatcttca tcatagtcta aaagggcagg ggcagatcct acaagattaa tatcaaagct
       61 acattcaaat gaaagagccg caccctctac aatccttaaa acaccctggt aaagatagtc
      121 tattatcttt tgattttctc ccctaatttc catttttaaa ttagccttat taggtataat
      181 atttcgtcca gatccagcct tcaaaactcc tacatttatc cttgataccc cgtttgaatt
      241 ttgggaaaga ttgttaaaat taagaattgc tgaacttgca gcaaggaggg cattgtggcc
      301 tttttcaggg cttgcaccag catggcttgt cctaccctta aaagatatat caagtttttt
      361 agtcgcaaga aagcccccac ttcctacaga aataaagcca cttggtaaat ccattcctat
      421 atgcatggct gccatataat caagcttatc aatcatagga tttcccatta aagaataagc
      481 tccccttact ccttcttctg caggttgaaa tattaccaaa atctttcccc taaatttatc
      541 cttattttgg gcaaagtatt tacaaaggct tattcctatg gctgtatggg catcatggcc
      601 acaagcatgc atggcaaaat cattctttga tgcaaaattt ttaatatttg gaatatgtcc
      661 cttagagtct gactccttta aatcatttgc atctatgtca aacctaaatc ctatattagg
      721 gccgggtctt ttgctatcaa tcaaggctat ggcccctgta aaagaatcta ggatttcatc
      781 ttttttatct tcattatcaa gacttatctt atctttataa agccttaaat ttttgctatc
      841 ataaaggcca agccttttat taaaatatat ttctttgcca taaaaaagct ttaagtctaa
      901 atctttaagc tcagaaatta tcttatctgt tgtcttaaat tccatccatc ctggctctgc
      961 attttggtga aaatatcttc ttaaatttat aagttcttta tcaatcattt ttttattaga
     1021 ttttcttatc taaaaatctc cctatatctt catatacttt tgctttttca tcttcattta
     1081 atacttcatg cttcatatg
//
LOCUS       DATGSI010000450         5901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_262900, whole genome shotgun sequence.
ACCESSION   DATGSI010000450 DATGSI010000000
VERSION     DATGSI010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262900"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            444..1553
                     /gene="chvE"
                     /locus_tag="VK031_06965"
     CDS             444..1553
                     /gene="chvE"
                     /locus_tag="VK031_06965"
                     /inference="COORDINATES: protein motif:HMM:NF040907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HLS53696.1"
                     /translation="MKRAFIILLTLVLAVSLVACGGETGEGETPKTPADGDASEGKLV
                     GIAMPTQSAPRWIEDGGNMKKILEEKGFQVDLQFGEDVVENQIAQIENMITKGADVLV
                     IASIDGEALTDVLAKAAEENIPVIAYDRLLMNSEHVDYYATFDNFQVGVEQGNYIVEK
                     MGLEDGEGPFNIELFGGSPDDNNAYFFYDGAMSVLQPYIDNGQLVVKSGQMGMDVVST
                     LRWDAATAQARMDNILTAHYTSDKVDIILSPYDPISLGCISSLKGVGYGTDDLPLPLI
                     TGQDADIPGVKSIIAGEQTQTIFKDTRELAKVAVEMVEAILEGKEAPVNDTETYDNGV
                     KVVPSYLLKPVSVDKDNYKEVLIDSGYYTEEDLGL"
     gene            1632..3161
                     /gene="mmsA"
                     /locus_tag="VK031_06970"
     CDS             1632..3161
                     /gene="mmsA"
                     /locus_tag="VK031_06970"
                     /inference="COORDINATES: protein motif:HMM:NF040905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HLS53697.1"
                     /translation="MAILKMENITKEFYGIKALDNVNLEVEEGEIHAIVGENGAGKST
                     LMNVLSGVHPYGTYDGKIIYEGQECQFNSIRDSEKLGIVIIHQELTLSPYMSAAENVF
                     LGNEQAKNGIIDWEKTTIEARKIFDKIGLDENPDTIVNTLGVGKQQLIEIAKALSKNV
                     KLLILDEPTAALNEDDSENLLNLLLEFKKSGITSILISHKLKEVAQVADRITILRDGA
                     TIETIENDEDLSEDRIIQGMVGRDIGDYYPEREHKIGEVFFEIKDWTVVDVLDENEKI
                     IDNININIRKGEVVGISGLMGAGRTEFAMSVFGQSFGKKTSGAIFKNGEELRINSVSQ
                     AIDNGIAYVTEDRKDYGLILIDSIKNNISLANFDKISKNSVINNNEEIKIAEDYRKNL
                     NIRSRDILQRLEDLSGGNQQKVVLSRWILSEPDLLILDEPTRGIDVGAKREIYTIINE
                     LAEQGKSILMISSELPELLEMCDRIYVMNRGRIVGELEGDEAIAENVMRCIMKDSREE
                     E"
     gene            3163..4329
                     /gene="mmsB"
                     /locus_tag="VK031_06975"
     CDS             3163..4329
                     /gene="mmsB"
                     /locus_tag="VK031_06975"
                     /inference="COORDINATES: protein motif:HMM:NF040906.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HLS53698.1"
                     /translation="MDKIKDLIKNNIRRYGMAIALVLIMGLFQVLTDGILLKPLNITN
                     LILQNSYILILAVGMLLVIITGNVDLSVGSVVALIGAISAHLMLKQNMSVVPVILISL
                     LIGALVGAWHGFWIAFIKIPAFIVTLSGMLLFRGLTLVILKGQTLAPFEESYRILSSG
                     FIKDIFGGQGLHITTLVIGVIFSLVYVYNEFRDRRNKIKYGFKVPSMNILIAKIIIVL
                     VSINLLTYSLAAYKGAPNVLVLLAFLVLVYSFVANKTIAGRYIYAIGGNEKAARLSGI
                     KTDMVLFWVYVNMGLLTALAGIVFTGRLNAAMPRAGTGFELDAIAACYIGGASASGGV
                     GTIIGAIIGGLVMGVLNNGMSILGIGIDWQQAIKGLVLLVAVAFDAYTKAKANR"
     gene            4351..5823
                     /gene="araA"
                     /locus_tag="VK031_06980"
     CDS             4351..5823
                     /gene="araA"
                     /locus_tag="VK031_06980"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HLS53699.1"
                     /translation="MGNYEFWFLIGSQHLYGEEVLEEVKKHGEIMVEGLNKSRLIPWK
                     IKLVDIGTTPDEITNIVEEANYDKNCAGIMTWMHTFSPSKMWIRGLTRLNKPYLHMNT
                     QFNREIPWDKIDMDFMNLNQSAHGDREHGFIAARLRLARKVVVGYWKDEDFQEKIGHW
                     MRSAIGVMESSKLKVARFGDNMREVAVTEGDKVEAQIKFGWAINYHPVGDLVQYIDRV
                     EDKEVDKLMEVYKEKYNLTTDRLDAVRYQARIELGMRSFLEEGNFGAFTTTFEDLHGL
                     KQLPGLAIQRLMVDGYGFGAEGDWKTAALTHIMKLMAKGLTKGTSFMEDYTYHLERDN
                     GLILGAHMLEVCPTIADDTPTVEVHQLGIGGKEDPARLVFDGKEGNVILASLVDLGGR
                     MRLIVNDAVAVKPLEDMPNLPVARVMWKPLPDLETSAEAWILAGGAHHSVMSYDLTAE
                     HMRDFAEMVGIEFIHINENTNIVELKKELLYNDLAWKLGI"
BASE COUNT         2086 a          738 c         1316 g         1761 t
ORIGIN      
        1 ttctaattaa gaagccttca tcttccaatt tttccaaatc tcttctgata gtaacctctg
       61 atacatctaa cattttgctt aattggttta cggatacttt cttttgttct attaaatagg
      121 ttttaatgat tcttattctc tcaattgcaa acatcataat tcctctcagt cttttgattt
      181 aacatgtttt ccctaatata aagtatatat taaatataat tgaaaaatca agttatatat
      241 tataataagc aaaagaaagg aatcgaaata aatcaaacat taatattgac ctaaacaaag
      301 gaatatgata caattcgaat aagaggataa ggttttccct ttttctcagc ttttgactac
      361 ttaaatagtc ttaatattca gtatatgaat taagtctttt aggtaatcgg agcaaaaata
      421 ttattatagg gggttcaaag aacatgaaaa gagcatttat tattttgctg actttagttc
      481 ttgcagtgtc actggtagcc tgtggtggag agacagggga gggcgaaact ccaaaaactc
      541 cagcagatgg agatgctagt gaaggtaaat tagtaggaat tgcaatgcct acacaatcag
      601 caccaagatg gattgaagat ggtggcaata tgaagaaaat ccttgaagag aaaggattcc
      661 aagtagatct tcaatttggt gaggacgttg ttgaaaacca aatagcacaa atagaaaata
      721 tgataactaa aggtgcagat gttttggtta ttgcatcaat tgatggggaa gctttaacag
      781 atgtacttgc aaaggctgct gaggaaaaca tcccagtaat tgcatatgac agactactaa
      841 tgaatagtga gcatgttgac tattatgcaa catttgataa tttccaagta ggggttgagc
      901 aaggaaatta tatagttgaa aagatgggtc tagaagatgg tgaaggacca tttaatattg
      961 aattatttgg tgggtctcca gatgacaaca atgcatactt cttctatgat ggagccatgt
     1021 cagtattaca accatatata gacaatggac aattagttgt aaaaagtgga caaatgggca
     1081 tggatgtagt atcgacacta agatgggatg ctgcaacagc tcaagcaaga atggacaaca
     1141 tacttactgc ccattatacg tctgataagg tagatataat actgtcaccc tatgatccaa
     1201 taagtttagg atgtatatct tcacttaagg gtgtaggata tggtacagat gatctacctc
     1261 taccacttat aactggtcaa gatgctgata tcccaggagt taaatctatt atagctggtg
     1321 aacaaactca aacaatattt aaggatacaa gggagttagc caaagtagca gtagaaatgg
     1381 tagaagccat actagaaggt aaggaagctc cagtaaatga tacagaaacc tatgataatg
     1441 gtgtgaaggt tgttccttcc tatctattaa agccagtatc tgtagacaag gataattaca
     1501 aagaagtatt aattgatagt ggatattata cagaagaaga tttaggattg tagcatgaaa
     1561 ttaatttggt gatagtcttg gtgatagtct ctaagactat cgccaagttt tctaaatagg
     1621 gggtgtcaaa gatggccata ttaaaaatgg agaatatcac taaggaattt tatggtatta
     1681 aggccttgga taatgtgaat ttagaggtag aagaaggtga aatccatgcc atagtaggcg
     1741 aaaatggggc aggaaagtca accttaatga atgtcttaag tggtgtacat ccttatggta
     1801 cttatgatgg gaaaataatt tatgaaggcc aggaatgtca atttaatagt ataagagata
     1861 gtgaaaaact aggtattgta ataatacatc aagaattaac cttaagcccc tatatgtcgg
     1921 ctgctgaaaa tgtattttta ggcaatgaac aagccaaaaa tggaattatt gattgggaaa
     1981 aaactacaat agaggcaagg aaaatatttg acaagatagg actagatgaa aatccagata
     2041 ctattgttaa cacattaggg gtaggtaaac aacagcttat agaaatagct aaggcattat
     2101 ctaaaaatgt taaattatta atacttgatg agcctacagc tgcattaaat gaagatgata
     2161 gtgagaattt attaaattta cttttagagt ttaaaaaatc tggaattaca tctatactaa
     2221 tatctcataa attaaaggaa gtggcccagg tagctgatag aataaccata ttacgtgacg
     2281 gtgctaccat tgaaaccata gaaaatgatg aggatttaag tgaagatagg attattcaag
     2341 gtatggtagg aagagatata ggtgattatt atccagagag agagcataag ataggtgaag
     2401 tattttttga aattaaagat tggactgtag tagatgtatt agatgaaaat gaaaaaatta
     2461 ttgataatat taatataaat ataagaaaag gtgaagtagt agggatttca ggcctaatgg
     2521 gtgctggaag aactgaattt gccatgagtg tatttggtca gtcctttggg aaaaagacct
     2581 ctggtgcaat tttcaaaaat ggagaagaac taagaataaa ttcagtaagt caagctatag
     2641 ataatggaat tgcttatgtc actgaggata gaaaggatta tggtttaatt ttaattgata
     2701 gtataaaaaa taatatcagt ttggcaaatt tcgataagat aagtaaaaac tcagtgataa
     2761 ataataatga ggaaataaaa atagctgaag attatagaaa aaacttaaat ataagatcga
     2821 gggatatttt acaaaggtta gaggacctaa gtggtggaaa ccagcaaaag gttgtattaa
     2881 gtagatggat attatctgag cctgatttat taatcctaga tgagcctaca agagggatag
     2941 atgtaggggc taaaagggag atatatacca taataaatga gttggcagag cagggaaagt
     3001 ctatattaat gatttcatca gagctgccag aattactaga aatgtgtgat agaatatatg
     3061 taatgaatag aggtaggatt gtaggagagc ttgagggaga tgaagctatt gcagaaaatg
     3121 taatgaggtg tattatgaag gatagtaggg aggaagagta atatggataa aataaaggac
     3181 ttaattaaaa ataatataag aagatatggc atggcaatag ctctagtcct aattatggga
     3241 ttattccaag tattaacaga tggaatacta ttgaagccat taaatattac caatttaatt
     3301 ttacaaaaca gctatatttt gatattagca gtgggaatgc ttttagtaat aattacaggc
     3361 aatgtggatt tatctgtagg ttcagtggta gccctaatag gtgccatatc cgctcattta
     3421 atgttaaagc aaaatatgtc tgttgtccca gtaatactta tatccttact aataggggca
     3481 ttagttggtg catggcatgg tttttggatt gcatttatta aaataccagc atttattgtt
     3541 actttatctg gaatgcttct ctttagagga ttaaccttag taatacttaa ggggcagact
     3601 ttagcacctt ttgaggaatc ctataggatt ttaagttcag gatttataaa ggacatattt
     3661 ggtggacaag gtttacatat aaccacccta gtaataggag taatcttctc cctagtatat
     3721 gtttataatg aatttaggga tagaagaaat aagattaaat atggatttaa agtaccttca
     3781 atgaatattt taatagctaa aattataata gtattagttt ctattaatct attaacctat
     3841 agtttggcag cctacaaggg tgctccaaat gttttagtat tgttggcatt tttagtacta
     3901 gtttatagct ttgtagctaa taaaaccatt gctggaagat atatatatgc tattggtgga
     3961 aatgaaaagg ctgcaagact ttctggtata aaaactgata tggtcctatt ttgggtttat
     4021 gtaaatatgg gtttactgac tgcattggcc ggtattgtgt ttacaggaag attgaatgca
     4081 gccatgccta gagcaggtac tggatttgaa ttagatgcaa tagctgcctg ttatataggt
     4141 ggagcctcgg cctccggtgg agtaggtact attataggag ccattatagg tgggcttgta
     4201 atgggagtat tgaataatgg tatgtcaatt ttaggtatag gtatagactg gcaacaggca
     4261 attaaaggtt tagtattact tgttgcagtg gcctttgatg catatacaaa ggcaaaggct
     4321 aatagataga aaagtaaggg ggtaaataat atgggaaact atgaattttg gtttctaata
     4381 ggaagtcaac atttatacgg tgaggaagtt ttagaagagg taaaaaagca tggagaaata
     4441 atggttgagg gcttgaataa atcaaggctt attccatgga aaatcaaatt agtagatata
     4501 ggaactaccc cagatgagat tacaaatata gtagaagaag caaattatga taaaaattgt
     4561 gccggaatta tgacctggat gcatacattc tccccatcta aaatgtggat taggggctta
     4621 accagattaa ataagcccta tcttcatatg aatacacaat ttaatagaga aattccctgg
     4681 gacaaaattg atatggactt tatgaattta aatcaatctg cccatgggga cagggaacat
     4741 ggatttatag cagcaagatt aagattggcc cgtaaagttg ttgtaggtta ttggaaagat
     4801 gaggatttcc aagaaaagat aggccactgg atgcgttcgg ccataggagt catggaatct
     4861 agcaagctaa aggtagccag atttggagac aatatgcgtg aagttgctgt aactgaggga
     4921 gataaggtgg aggctcaaat taaatttggt tgggcaataa attatcatcc tgtaggagat
     4981 ttagttcaat atatagatag ggtagaagac aaagaagtgg ataaattaat ggaggtttac
     5041 aaggaaaaat ataatttgac cactgataga ttagatgcag taagatacca ggctagaata
     5101 gagttgggga tgagaagctt cctagaagag ggtaattttg gagcctttac cactaccttt
     5161 gaagacctac atggattaaa gcaattacca ggtttggcca ttcaaagatt aatggtagat
     5221 ggctatggat tcggtgctga aggagattgg aaaactgcgg ctctaaccca tattatgaag
     5281 ctgatggcaa agggccttac aaagggtacc agctttatgg aagactatac ctatcatcta
     5341 gaaagggata atggtctgat cctaggtgcc catatgttgg aagtttgtcc aactatagct
     5401 gatgatactc ctactgttga ggtacatcag ttaggtattg gaggcaagga ggatcctgca
     5461 agactggtct ttgatgggaa agagggcaat gtaattttgg cctccttagt agacttgggt
     5521 ggtagaatga ggctgattgt aaatgatgca gtggcagtaa aacctttaga agacatgcca
     5581 aatctaccgg tggctagggt tatgtggaag cctctgccag atttggaaac atcagctgaa
     5641 gcttggattt tggcaggtgg agcccatcac tcggtaatga gctatgattt aactgctgag
     5701 catatgaggg attttgctga aatggttggt atagagttta ttcatataaa tgaaaatacc
     5761 aatatagtag agcttaaaaa ggagctacta tacaatgatc ttgcttggaa gttaggaata
     5821 taagggggat atcatgaaaa ataaaaaata tattgatgat attcattctg gtaaaactag
     5881 cttagggatt gagttaggtt c
//
LOCUS       DATGSI010000451         1097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_312539, whole genome shotgun sequence.
ACCESSION   DATGSI010000451 DATGSI010000000
VERSION     DATGSI010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312539"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..550
                     /locus_tag="VK031_06985"
     CDS             107..550
                     /locus_tag="VK031_06985"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HLS53700.1"
                     /translation="MKRINLWTGIITLLIGLVWAISALKLGLINKGQIGPGLWPFIVG
                     VSLSFLGLLLILSRKSKEEFSADLPKGERLLRLLIILVSFTIYIFLINFLGIFISNIL
                     FLFVLIRFWGEYSWIKVGLLSVSIAISLHLIFTVWLNMPLPTGLF"
     gene            562..>1097
                     /locus_tag="VK031_06990"
     CDS             562..>1097
                     /locus_tag="VK031_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HLS53701.1"
                     /translation="MTIINDLINGFGTALTLNNLLFCLIGVTLGTLIGVLPGLGPTAG
                     IAVLIPLSAGLDPTTAIIMLAGIYYGSMYGSSTSAILINTPGQGAAVLTAVEGFELAK
                     QGRAGPALGMSAIASFVGGTISLFGLIFLAPVLANLALGFGPSEYFALMVFGMTIVVS
                     LSGTSLVKGLISAFIGLLV"
BASE COUNT          326 a          155 c          227 g          389 t
ORIGIN      
        1 aaaattaaaa cgtctgagaa atgattatat gttatgaaaa agtataaaaa ctaaaataag
       61 acaacttaaa ccaataaatt tttttagatt ccgggggcgg agtttagtga aaaggattaa
      121 cttatggaca ggtataatta ctttattaat tggacttgta tgggcaatta gtgctttaaa
      181 gttaggtctt attaataagg ggcaaatagg accgggctta tggccattta ttgtaggagt
      241 atctcttagt tttttagggt tactacttat actgtctaga aaatcaaaag aagaattttc
      301 agcagatttg cctaaaggtg aaagattatt acgtttatta atcatcctag tatcatttac
      361 tatttatata tttttaataa acttcttagg gatttttatt agtaacattc tatttctatt
      421 cgtattgatt agattttggg gagaatattc ctggattaaa gttggtttac tgtcggtttc
      481 gatagcgatt agtttacatt tgatttttac agtatggtta aatatgcccc ttccaacagg
      541 gttattttaa ggaggtaatt aatgactata ataaatgact taataaatgg ttttgggaca
      601 gcactaacat taaataattt actattttgc ttaattggag ttaccttagg aacactgatt
      661 ggagtgctcc caggtttagg gcctacagct ggaattgcag tattgatacc tctatcagct
      721 ggcttggatc ctacaactgc aattataatg ctggcaggga tttattatgg gtcaatgtat
      781 ggtagttcaa cgagtgcaat tttaattaac actccaggac aaggcgcagc agtcttgact
      841 gctgtagaag gtttcgaact agctaaacaa ggaagagcag gtcctgcctt aggaatgtca
      901 gcgatagcat cattcgtagg agggactatt agtttatttg ggttaatctt tttggcacct
      961 gtcctagcta acttagcact tggtttcgga ccctctgagt attttgctct gatggtgttt
     1021 ggcatgacca ttgtagttag tctctctggt acctctctag taaaaggact aattagtgct
     1081 tttataggtc tgttagt
//
LOCUS       DATGSI010000452         1967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_221286, whole genome shotgun sequence.
ACCESSION   DATGSI010000452 DATGSI010000000
VERSION     DATGSI010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221286"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VK031_06995"
     CDS             <1..323
                     /locus_tag="VK031_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53702.1"
                     /translation="EIKRIVEKLYEEEFKSGEVTLKLRISLVEDFKEFPVKDELGFKD
                     NKIVYFNRPVIEHHVNTTLKKQYKEKGKISLDSQIEKNAEDVFVLVPIEEPQLDLFKD
                     EFLK"
     gene            347..931
                     /locus_tag="VK031_07000"
     CDS             347..931
                     /locus_tag="VK031_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HLS53703.1"
                     /translation="MKSRLMNDTKVMLANVRLSYPALFEPRGFEGQEPKYSASLIIPK
                     SDTESLKVIKQAIENAKEEGKERGIWKGGKIPANLRLPVRDGDEERPDDEAYADSYFI
                     NANSKYAPVVVGKLKDKATGKAITLGEDDVYPGCYVNVTINFYGYSAAGNNGIAAGLG
                     NVQKEADGENLGGRSSAESDFEFEEVDSDDDFLF"
     gene            912..>1967
                     /locus_tag="VK031_07005"
     CDS             912..>1967
                     /locus_tag="VK031_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53704.1"
                     /translation="MMTSCSKIKTLNIDIETYSDEDLGKVGVYKYVDSPAFKILLFAY
                     SVNEGEVKVVDIENGEKIPDEIMKALVDEDIKKYAFNAQFERVCLNHFYGIESTNWTC
                     TMIKAWYCGITGGLASVGNAIGLSDDKAKMQEGKRLINKFSKPTKIKQMNLLEDNDWK
                     LFKEYNKRDVEVELAIKKKLDRFEIPDWEMELYALDQKINDRGIKLDVDMVLNAIKID
                     DELTELATHKFKEITGFDNPNSIKDIRQFIKDKTGVEVKSLAKGVMDDLLHRFRGFPE
                     IIEVLKIRQQLSKTSTAKYYTMRDVIMTDGRSRGNIQHYGASRTGRWAGRLIQVHNLP
                     RNYIKDLDTARNIIKYGD"
BASE COUNT          742 a          224 c          436 g          565 t
ORIGIN      
        1 ttgagataaa gaggattgtt gaaaaacttt atgaagaaga gtttaaatca ggggaggtta
       61 ctttaaagtt gaggattagt ttagttgaag actttaaaga atttccagtg aaagatgagc
      121 taggatttaa ggataataag attgtttatt ttaatagacc tgttattgaa catcatgtaa
      181 acactactct taagaaacaa tacaaagaaa aaggaaaaat aagtttagat tctcaaattg
      241 aaaagaatgc cgaagatgtt tttgtattag ttccaataga agaaccacag ttagatttat
      301 ttaaagatga atttttaaaa taaaaaatta aagggagaga ttaataatga aaagtagatt
      361 aatgaatgat actaaagtta tgttagctaa tgtaaggtta agttacccag ctctatttga
      421 acctagggga tttgaaggtc aggaaccaaa gtattcggct agccttatta tccctaagag
      481 tgatacagag agcttaaagg taatcaaaca agctattgaa aatgctaaag aggaaggcaa
      541 agaaagggga atttggaaag gaggtaaaat tccagctaat ttaagattac ctgtacgaga
      601 tggagacgaa gaaagaccag atgatgaagc ttatgcagat tcctacttta ttaatgctaa
      661 ctcaaaatat gctcctgtag ttgtaggtaa attaaaagat aaagctactg gtaaggcaat
      721 tacattaggt gaagatgatg tttatcctgg ttgctatgta aatgtaacta ttaactttta
      781 tggttacagt gctgcaggga ataatggaat agcagcaggg ttaggaaatg tgcagaaaga
      841 ggctgatgga gagaacttag gtggaagaag ttcagctgaa agtgattttg aatttgaaga
      901 agtggattct gatgatgact tcttgttcta agataaagac attaaatata gatatagaaa
      961 cctatagtga tgaagactta gggaaggtgg gagtttacaa gtatgtagac tccccagcct
     1021 tcaaaatact tctatttgct tattctgtaa atgaaggcga agtcaaagta gtagacattg
     1081 aaaatggaga gaaaatacct gatgagatta tgaaggctct agttgatgaa gatattaaga
     1141 aatatgcttt taacgcacag tttgagagag tatgtttaaa ccatttttat gggattgaat
     1201 ctactaactg gacttgtact atgattaaag cttggtactg cggaattacc ggtggtcttg
     1261 cttctgtggg taatgctata ggtctttcag atgataaggc taagatgcag gaaggtaaaa
     1321 ggcttatcaa caagtttagt aaaccaacaa aaataaagca aatgaattta ctagaagata
     1381 atgattggaa actgtttaaa gaatataaca aaagagatgt tgaggtagaa ctagccatta
     1441 aaaagaaact agataggttt gaaattcctg attgggagat ggagctatat gctctagacc
     1501 agaaaattaa cgatagaggg attaaattgg atgttgatat ggtccttaat gctataaaaa
     1561 ttgatgacga attgaccgaa ttagccactc ataaatttaa agaaattacc ggctttgaca
     1621 atcctaattc tattaaagat ataagacagt ttattaaaga caagactggt gtagaggtta
     1681 aaagcctagc caagggagtt atggatgatt tacttcatag gtttagagga ttcccagaaa
     1741 ttatagaagt acttaaaatt agacagcaac tctcaaagac ttctacagct aagtactata
     1801 ctatgaggga tgttattatg actgatggta ggagtagagg taatattcag cattatggag
     1861 ctagtagaac aggcaggtgg gcgggtagat taattcaagt tcataatcta cctaggaact
     1921 atataaagga tctagacaca gcaagaaaca taataaagta tggagat
//
LOCUS       DATGSI010000453         1152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_409651, whole genome shotgun sequence.
ACCESSION   DATGSI010000453 DATGSI010000000
VERSION     DATGSI010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409651"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="VK031_07010"
     CDS             <1..1106
                     /locus_tag="VK031_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53705.1"
                     /translation="TIPFIHKKLSKYIFIGQRLYHLIGGAGGSGKSAWVDLNYAINPA
                     IWMAKYGQQEDIKLKIILRSMERSKKHRIAKWVCMRLYIDHGILIDVPSLFGWGLKKN
                     YISDELYEKIRGAVEQTEQLEDIIEVVDGIDHPTGIYTHLRKEALSKGTLFRYTDEDV
                     FVKQRGSSISKAEKAECPRASKYEPIYIPDNEREVTIFIVDHLQALKRERGFSDKENL
                     DKMSEYSRVLRDLYGFSPVMVSQLNRGVSDTMRRVKTDLLPEDKDFSGSSNLYNDCDM
                     AAILFNPYKYGMKDLEKWRINDLIDKKYGINRFRSYHLLKNTYGPDNVIFGYNFIGEN
                     GMYKELKSPSDMQPQDYQNSAYPKFNKRITGYE"
BASE COUNT          409 a          140 c          247 g          356 t
ORIGIN      
        1 ttactattcc ttttatccac aagaagctat ctaagtacat atttataggt caaaggttat
       61 atcacttgat tggaggagca ggaggatctg gaaagagtgc atgggtagat cttaactatg
      121 ctattaatcc tgctatctgg atggctaaat atggtcaaca agaagatata aaattgaaga
      181 ttattctccg atctatggag agaagtaaga agcatagaat agctaagtgg gtatgtatga
      241 ggttatatat tgatcacggt attctcatag atgtgccttc tctgtttggt tggggtctta
      301 agaagaatta tatctctgat gaactatatg aaaagataag aggtgcggta gaacaaacag
      361 agcagttaga agatataata gaagtagttg atggaattga tcatcctaca ggaatctata
      421 cacatcttcg taaagaagct cttagtaagg gtacattatt tagatataca gatgaagatg
      481 tatttgttaa gcaaagagga tcttctatta gtaaagctga gaaggcagaa tgccctagag
      541 cttctaagta tgaacctatt tatattcctg acaatgaacg ggaagtaacc atttttatag
      601 ttgatcatct tcaagctctt aaaagagagc gtggatttag tgataaggag aatttagata
      661 agatgtctga atactccaga gttcttagag atttatatgg cttttctcca gtaatggtta
      721 gccagttgaa tagaggagtt agtgatacca tgagaagagt taaaacagat cttctacctg
      781 aagataaaga cttctctgga tcaagtaact tatacaatga ttgtgatatg gcggctatac
      841 tcttcaatcc ttataagtat ggaatgaagg atttggagaa gtggagaata aatgatttga
      901 tagataagaa gtatggtatt aatagattta gaagttatca tcttcttaag aatacttatg
      961 ggccggataa tgtgatattt ggatataatt ttataggtga aaacggtatg tataaggagt
     1021 taaagtctcc aagtgatatg caacctcaag attatcaaaa ttcggcttat ccaaaattta
     1081 ataaaagaat aacaggttat gaatgaaaaa ttaaaagaac aacatttagc gacgggaaat
     1141 gttgttaaac tt
//
LOCUS       DATGSI010000454         1146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_221463, whole genome shotgun sequence.
ACCESSION   DATGSI010000454 DATGSI010000000
VERSION     DATGSI010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221463"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1146)
                     /locus_tag="VK031_07015"
     CDS             complement(<1..>1146)
                     /locus_tag="VK031_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HLS53706.1"
                     /translation="GNNFVDGEAFELPFSLEELGLKEKVYYPLMKELIDNYDSEEELK
                     KAIKDRVKELSPKHITIDDIVASINYEFNLFNGIGYTDDIDHLGNRRLRSVGELLQNQ
                     FRIGLSRMERAVREKMTIQDIDVVTPQSLINIRPVTAAINEFFGSSQLSQFMDQTNPL
                     AELTHKRRMSALGPGGLSRDRAGFEVRDVHHSHYGRMCPIETPEGPNIGLITSLTTYA
                     RINEYGFLEAPYRRVKKGTGQITGEIDYLTADLEDEFIIAQSNEELTPDKKFANKRVV
                     GRGKNGVVDIFPSTEVDYMDVSPKQIVSVGAAMIPFLENDDANRALMGANMQRQAVPL
                     IRAEAPIVGTGIEYRAARDSGVVVIAQHEGIVEKVSSSEIVVKRTED"
BASE COUNT          303 a          265 c          168 g          410 t
ORIGIN      
        1 ttgtcttctg ttcttttaac cacaatctca ctagagctta ctttttctac aatcccttca
       61 tgctgagcaa ttaccactac acctgagtct ctagctgccc tatattctat accagttccc
      121 acaatgggag cctcagccct aattagtggt actgcttgac gttgcatatt tgcacccatc
      181 aatgctctgt tggcgtcatc attttccaaa aatggaatca tagccgcccc aacagataca
      241 atttgttttg gggaaacatc catatagtca acttcagtac ttgggaaaat atctaccaca
      301 ccatttttac ccctgcctac aactcttttg ttggcaaatt tcttatctgg tgttagttct
      361 tcatttgact gggcaataat aaactcatct tctagatctg cagttagata atctatttct
      421 cctgttatct ggcctgtacc ttttttaacc cttctataag gtgcttctaa aaatccatat
      481 tcattaatcc ttgcataagt agttaatgaa gtaatcagtc ctatatttgg cccctcagga
      541 gtttcaattg gacacattct accgtagtga gaatggtgta cgtctcttac ctcaaatcct
      601 gccctgtctc tacttaggcc acctggccct aaagctgaca ttcttctttt atgggttaat
      661 tcggccaatg ggttggtctg atccataaac tgtgacaact ggctgctgcc gaagaattca
      721 ttaatggcag ccgtcactgg cctaatattg atcaaagatt gaggtgtaac aacatctata
      781 tcttggatgg tcattttttc tctgactgct ctttccatcc ttgaaagtcc aatcctaaat
      841 tgattttgca acaattctcc tacagatctt aatcttctat tgcctaaatg gtctatatca
      901 tctgtatatc ctatcccatt aaataggtta aactcataat ttatacttgc aactatatcg
      961 tctattgtaa tatgtttagg ggataattct ttaactctat ccttgattgc ctttttcagt
     1021 tcctcttcgc tatcatagtt atcaattaac tctttcatta aaggataata tactttttct
     1081 tttagaccta gttcctcaag agaaaatggt aattcaaagg cctctccatc aacaaaatta
     1141 tttcct
//
LOCUS       DATGSI010000455         5077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_693418, whole genome shotgun sequence.
ACCESSION   DATGSI010000455 DATGSI010000000
VERSION     DATGSI010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693418"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VK031_07020"
     CDS             complement(<1..276)
                     /locus_tag="VK031_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HLS53707.1"
                     /translation="MAKDIKFGENARRSMEKGINQLADTVKVTLGPKGRNVVLDKSFG
                     APTITNDGVTIAREIELEDKYENMGAQLVKEVATKTQDVAGDGTTTAT"
     gene            complement(399..674)
                     /locus_tag="VK031_07025"
     CDS             complement(399..674)
                     /locus_tag="VK031_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HLS53708.1"
                     /translation="MNLKPLGDRLVIKKVEVEETTKSGLIVPDSAKEKPQIAEVLALG
                     DKILEDEKNQLKVGDKVIFSQYAGTEIKMDGEEVTILKLNDILAIVE"
     gene            complement(866..1561)
                     /locus_tag="VK031_07030"
     CDS             complement(866..1561)
                     /locus_tag="VK031_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HLS53709.1"
                     /translation="MLGTIVNVSTIVVGSIIGTLISRGIKDNYKNTIMDGISLCVLII
                     GIISGIKTENIILAIGSIVLGSLIGERISIEKRLDRLGQKMEKRLGDDRGDFAKGFVT
                     ASLVYCIGAMAIVGSLESGLTGNHETLFAKSILDGITAIIFSSTFGIGVAFSALPVLI
                     YQGSITLLASLVKDILTAQIINEMSAVGGILIMAIGINTLGIKKIKVGNMLPAIFLPI
                     IYYIIMDLLVFIR"
     gene            complement(1630..1890)
                     /locus_tag="VK031_07035"
     CDS             complement(1630..1890)
                     /locus_tag="VK031_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HLS53710.1"
                     /translation="MLKNYMEIAVDMVLKGVLKSKELECMCEKCLDDIRAITLNKLKP
                     MYVVSDEGVLYSKLQELSVQFRADILRELTQAIAIVEESPRH"
     gene            complement(1910..2785)
                     /locus_tag="VK031_07040"
     CDS             complement(1910..2785)
                     /locus_tag="VK031_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HLS53711.1"
                     /translation="MEKYKIKEQRNKIILKNMKDFNPKHIFECGQAFRWIKEEDNSYT
                     AISKGRVVNVKREDGDVVFNDTNLEDFKNIWYHYFDLETDYGQIKRELAKDPILEEAI
                     EFGDGLRILNQEPFETTISFIISANNQIPRIKRSIELISKHFGKQIKEDYYSFPTADK
                     LAQVQVDELKEISRVGFRAQRIVDTSRLVAEGAIDINSIYKLSKEEGKELLMTLPGVG
                     PKVSECILLFAYKKREAFPVDVWVKRVMEHFYLKEDRKVQDIEKEGARLFGDLAGFAQ
                     QYLFYYARELGIGRN"
     gene            complement(2921..3550)
                     /locus_tag="VK031_07045"
     CDS             complement(2921..3550)
                     /locus_tag="VK031_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HLS53712.1"
                     /translation="MTKDSQVSITVIRRLPKYHRYLSDLLDKGVNRISSQELSRLTGF
                     TASQIRQDLNNFGGFGQQGYGYNVEELYRELGKILGLDKQYNVVLTGAGNLGYAISHY
                     HGFKEAGFNIIGIFSRYTESKIFNGKDIQIRHIDGLEDFIKENNVSIGIITTSKENAQ
                     NVADIYVRAGIKGIWNFAPVDLVVPEDVILETVRLSESLFTLSYYIKDL"
     gene            3689..3904
                     /locus_tag="VK031_07050"
     CDS             3689..3904
                     /locus_tag="VK031_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53713.1"
                     /translation="MASKDQLRKIAIYCDEYRNRIHDEEVSLKNMAQAEHVSCTNCVH
                     FTREHTCELDLIDKVLSSLAMEQDLKS"
     gene            4008..>5077
                     /locus_tag="VK031_07055"
     CDS             4008..>5077
                     /locus_tag="VK031_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS53714.1"
                     /translation="MPVLACRNISKTYIIDKILDGVSFTIEEGDKVGLIGLNGSGKTT
                     LFNILAGEISKDSGDIFIQKDLKIGYLKQHTSISSQKQVFEECLEIFQDLISMEKKLR
                     NLEEEMSQIGSSGQSEKLDKLMNRYGHLSEKFTALNGYGYKSDIRGVLVGLGFEVEDF
                     SKKVDELSGGQKSRLALAKLLLEKPDLLLLDEPTNHLDIGAIDWLEKFLKDYEGAALI
                     ISHDRYFLDNTVNRIFNLENKKINIYNTNYTKFMEQRKKDLEVLQKQYENQQREIKRQ
                     EEIIQRFMNYGNARYIRQAQARQKLLEKIKQVERPVEAEQTRIKFEPKLKSGRDVLEV
                     KELAKSFGDRKLFSQVNFQIYRG"
BASE COUNT         1608 a         1002 c          719 g         1748 t
ORIGIN      
        1 agttgcagtt gtagtaccgt ctcctgccac atcttgagtt tttgtagcca cttcttttac
       61 taactgggct cccatattct catacttatc ttctaattca atttctctag caatagtaac
      121 accatcatta gtaatagttg gtgcaccaaa ggatttatct aaaacaacat ttctcccttt
      181 aggtccaagg gttaccttta ccgtatcagc caattgattg attccttttt ccatagaacg
      241 acgtgcattt tcgccaaatt taatatcttt tgccatattt tatccacact cctatcatca
      301 aagtatttca taagctattg gtttacaatc catatttaac cacttgcaat ttgctttagg
      361 ctctttccta ctatttctgt gaaattattt aacttgtttt actctacaat tgctaaaata
      421 tcatttagct ttaggatagt tacttcttca ccatccattt ttatttctgt tcctgcatat
      481 tgggagaaga ttactttgtc accaactttt aattggtttt tctcatcttc caaaatctta
      541 tcacccagtg caaggacttc agcaatttgt ggtttttcct ttgcagaatc aggtacaatt
      601 aaaccacttt ttgttgtttc ctcaacttct acttttttta ttactaatct gtctcctaat
      661 ggctttagat tcatctaata ttacctcctt aaattttcct ctttgttatt agcactcacc
      721 ttcgctgagt gctaatctgc acaatcttaa tgttacccta aaaaaaacca gttatcaagt
      781 gtcatatttg gacatatata taattaaaat atttatatat ataatctttt ttggtttcgc
      841 ctattttttc tctcttttcc tttgtttatc taataaatac taataaatcc ataattatgt
      901 aatatataat aggtaaaaag atggctggta acatgtttcc cacctttatt tttttaatac
      961 ctagggtatt tatgcctatg gccataatca ggattccacc aactgcagac atttcattta
     1021 ttatttgagc tgttaaaata tccttaacta aactggctaa aagagtaata ctgccctgat
     1081 aaattaacac tggtagagca gaaaaggcta ctcctatccc aaaggtggaa gaaaagatga
     1141 ttgcagttat accgtctaaa atggatttgg caaataaggt ttcatgatta ccagtaagcc
     1201 cagattctaa ggagcctact atagccatag cccctataca atatactaat gaggccgtta
     1261 caaagccctt agcaaagtct cccctatcat ctcctagcct tttctccatc ttctgcccca
     1321 gcctgtctag cctcttttct atggatattc tttccccaat taagcttcct aatactatac
     1381 ttccaattgc taggataata ttttctgttt taataccact tataatccca ataataagga
     1441 cgcacaagct aatgccatcc ataatagtgt ttttgtaatt gtcttttatt cccctactta
     1501 ttaatgtacc tataattgat cctactacaa tagtagatac attaactata gttcccaaca
     1561 tagtcccacc tcaattatat taaaaataaa ggctggtaaa ataccagcca ggataaaccg
     1621 tatagtaatt taatgtctag ggctttcctc aacaatggca atggcctgag taagttccct
     1681 taggatatct gccctaaact gtacacttag ttcctgcaac ttactatata aaactccctc
     1741 atctgatacc acatacatag gttttagctt gtttaaggtt attgccctta tatcatctaa
     1801 acatttttca cacatacatt ccagttcctt tgattttaac acacctttca aaaccatatc
     1861 aactgctatt tccatataat ttttaagcat gcccatcctc ctttgaacct taattcctcc
     1921 caatccccag ttccctggca taataaaaca aatattgctg ggcaaatcca gccaagtctc
     1981 caaaaagtct ggccccttcc ttttctatat cctgaacctt cctatcctcc ttcaaataga
     2041 aatgttccat tactctcttt acccaaacat ctactggaaa tgcctctctt tttttatagg
     2101 caaaaagtaa aatacattca ctaaccttag gtcccacacc aggcaaggtc ataagaagct
     2161 ctttcccttc ctctttactt agcttataga tactatttat atcaatagcg ccctcagcaa
     2221 caagcctgga tgtatctact attctctggg cccgaaaacc tacccttgaa atttctttta
     2281 attcatccac ttgaacctga gccaatttat ctgctgttgg aaaactgtag taatcttcct
     2341 taatctgctt cccaaagtgt ttggaaatta attctatgga tctttttatc ctgggaattt
     2401 ggttgttggc agatattata aaggaaattg tagtttcaaa gggctcctgg tttaaaattc
     2461 ttaagccatc accaaattca atggcctctt ctaaaatagg atctttggct agttcccttt
     2521 ttatctgccc ataatcagtt tctaagtcaa agtagtgata ccaaatattt ttaaaatctt
     2581 ctagatttgt atcattaaag accacatccc catcttccct tttaacatta accaccctgc
     2641 ctttagatat ggcagtataa ctattatcct cttccttaat ccacctgaag gcctgcccac
     2701 attcaaatat atgtttagga ttaaaatcct tcatattttt cagaattatt ttattccttt
     2761 gttcttttat cttatatttt tccatcttta tctccttatc atttgtctta ctaatatgat
     2821 aacatttaaa tcaaagttaa tacatgttta accaagataa aagccttata aactactata
     2881 gcataaattt aaagggccct aacagcccct aaaatttatg ctatagatcc ttaatgtaat
     2941 atgataaggt aaataaactt tcgcttaacc ttactgtttc caaaataaca tcctctggaa
     3001 caaccaggtc cacaggggca aaattccaaa tgcccttaat acctgcccta acataaatat
     3061 ctgcaacatt ttgggcattt tcctttgatg tagtaataat acctatactt acattattct
     3121 cctttataaa atcttccagc ccatcaatgt gcctaatttg aatatccttt ccattaaata
     3181 tcttgctttc tgtatatcta ctaaatatac ctataatatt aaaacctgct tctttaaaac
     3241 cgtgataatg acttatagca taccctaggt tacctgcacc tgttaatacc acattatatt
     3301 gcttatccag tcctaaaatt tttcctaatt ccctgtacaa ttcttccaca ttataaccat
     3361 atccctgctg cccaaatcca ccaaaattat ttaaatcttg tcttatttgg gaggctgtaa
     3421 atcctgttaa cctactaagt tcttgggagg atattctatt tacaccttta tccagcaaat
     3481 ctgatagata tctatggtat tttggcaatc ttctgataac tgtaatggat acctgagaat
     3541 cctttgtcaa ctataacacc acccaatctt ttctttttat attattacaa ttataacatt
     3601 atgttaaaat atagtcaatt taacaagctt gaactttttg aatatttata cttcttttcc
     3661 acataatatt tacaaaagga gtgattttat ggctagtaaa gatcaattaa gaaaaatagc
     3721 tatttattgt gatgaatatc gtaaccgcat ccatgatgag gaagtatctt taaaaaacat
     3781 ggcccaggct gaacatgtat cttgtaccaa ttgtgtccac tttaccagag agcatacttg
     3841 tgaattagat ctaatagata aggttttatc atctcttgcc atggagcagg atttaaaatc
     3901 ttaattccca attgttagac cctttcatat acctccctaa gctaaatttt tcaataaaat
     3961 attattttat gatacaatat tgatactaaa gcttaggggg aacttttatg cctgtattag
     4021 catgtagaaa tattagtaaa acttatatta tagataaaat cctagatggg gtaagcttta
     4081 ccattgagga gggagataag gtaggtctta ttggcctaaa tggctctggt aagaccaccc
     4141 tttttaatat attagccggt gaaatcagta aggacagcgg ggatattttt attcaaaaag
     4201 acttaaagat cggctattta aaacaacata ccagtataag tagccaaaaa caggtctttg
     4261 aagaatgttt agaaatcttc caagacctga tcagtatgga aaaaaagctt aggaacttgg
     4321 aagaggaaat gtctcagatt ggttcaagtg gacaatcaga aaaattggac aaattaatga
     4381 atagatatgg tcatttatct gagaaattta ctgctttgaa tggttatgga tataagtctg
     4441 atattagggg agttttggtt ggattaggct ttgaagttga agatttttct aaaaaggtgg
     4501 atgagttaag tggagggcaa aaatccagac tggccctggc caaactactt ttggaaaaac
     4561 cagatctttt acttcttgat gagcctacca accatctgga cataggggcc atagattggt
     4621 tagaaaaatt tttaaaagac tatgaaggtg ctgccctgat tatatcccat gataggtatt
     4681 ttttagacaa tactgtcaac aggattttta atttagaaaa taagaaaatc aatatttata
     4741 atactaatta taccaagttt atggaacaga gaaaaaagga ccttgaagtt ttgcaaaaac
     4801 aatacgaaaa ccaacaaaga gaaatcaaaa ggcaggaaga aataatccaa cgctttatga
     4861 attatggtaa tgccagatat attaggcagg cccaggccag acaaaaatta ttggaaaaaa
     4921 ttaagcaggt ggaaagacct gtggaggctg agcaaactag aataaaattt gagcctaagt
     4981 taaagtcagg aagggatgta ctggaagtta aagagttggc aaaaagtttt ggagatagga
     5041 agcttttttc tcaggtaaac tttcaaatat atagggg
//
LOCUS       DATGSI010000456         1820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_30130, whole genome shotgun sequence.
ACCESSION   DATGSI010000456 DATGSI010000000
VERSION     DATGSI010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30130"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VK031_07060"
     CDS             complement(<1..503)
                     /locus_tag="VK031_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53715.1"
                     /translation="MRVGVKGKEAIQILDADVAIQSNESCGRTASGDTSFSQAILEVV
                     PLADFQNYCPKDFEKKWMSQYLTKGQHYTELLFAEDMMNVRASLLAKANEKLIWQGDT
                     DEVDTNLNKFDGVLKQLEDAGVTATDLSAETDVVAQLQAIVAGADADGVGSGDFTIFL
                     GKDKATA"
     gene            complement(675..1349)
                     /locus_tag="VK031_07065"
     CDS             complement(675..1349)
                     /locus_tag="VK031_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53716.1"
                     /translation="MAKKVNLTSKALETLKALFSSVSLGFSQFEDGEGNIYQYSVLEV
                     GAEVFIATAEGSEPAPDGEYKVDEVTSIKVEDGLITEVNKEEKEESEEEEVVEVDVLS
                     DFKKALSSKLKGTGKFSDEELAELLEYEEDLEELVEEVAETEEEEELLGEVVSGITNL
                     ITENKEVKDEVEELKEEIEALKEGFSKLASEPVKTSKKVSFNSFDNDNKEGKATLQSV
                     FSRITK"
     gene            complement(1399..>1820)
                     /locus_tag="VK031_07070"
     CDS             complement(1399..>1820)
                     /locus_tag="VK031_07070"
                     /inference="COORDINATES: protein motif:HMM:NF025901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XkdF-like putative serine protease
                     domain-containing protein"
                     /protein_id="HLS53717.1"
                     /translation="DKPIYREIDGKQFYVVFSKETIKQIALNLFKKGYNTKMNVEHTD
                     EDADSFIYQSYIVDTEKGINAPKGLEDTPDGSWIIGAKVNSKELWEDIKEGKRNGFSV
                     EGLFAIKEFKEAFSKQDRPMATKVKEMNKFLNNFLGN"
BASE COUNT          510 a          405 c          237 g          668 t
ORIGIN      
        1 taagctgtag ctttgtcttt tcctaggaag atagtaaagt ctccagaacc taccccgtca
       61 gcatcagcac ctgcaactat tgcctgtaat tgagcaacaa catcagtctc agctgaaagg
      121 tcagtagcag ttacacctgc atcctccaat tgttttaata caccatcaaa cttattaagg
      181 ttagtgtcaa cttcatcagt atctccctgc caaatcaatt tctcgttagc cttagccaac
      241 aaagaagctc taacgttcat catatcttct gcgaacaaaa gctctgtgta atgttgtcct
      301 ttagttaggt attgagacat ccactttttc tcaaaatctt taggacaata gttttggaaa
      361 tcagccaaag gcacaacctc taaaattgcc tgtgagaaag aagtatctcc tgaagcagta
      421 cgtccacaac tctcgttaga ctggattgct acatcagcat ctaaaatctg aatcgcttct
      481 tttcccttaa ctcctactct caaagcacca atgctattaa ggtactttac agtatcattt
      541 tctgtaattg cacgtgtaac aaactctttg gagttctcat taatgtagtc tgtaagtgcg
      601 gttaaatcta atgccatttt tttctaattt aatttctatt tataatagtt atcctttact
      661 ctgttaatgt ataattactt agttatacga ctaaatacgc tctgcaaagt agctttacct
      721 tctttattgt cgttatcaaa agaattaaaa gatactttct tagaagtttt tactggttca
      781 gaagccaatt tagagaatcc ttctttaaga gcttcaatct cctctttaag ttcctctact
      841 tcgtctttaa cttccttatt ttctgtaata aggttagtga tgccagaaac tacttctcct
      901 agtagctctt cttcctcttc ggtctctgct acttcttcta ccaattcttc caaatcttct
      961 tcgtactcta aaagttctgc taactcttca tcagagaatt taccagtacc ttttagttta
     1021 gaagataaag cctttttaaa gtcagataac acatctactt caactacttc ctcttcttca
     1081 gattcttctt tctcttcctt attaacctct gtaataagac catcttctac tttaatagaa
     1141 gtcacttcat caaccttata ctctccatct ggtgctggtt cagacccttc agcagtagca
     1201 atgaatactt ctgcacctac ttccaatacg gaatactggt agatattacc ttccccatcc
     1261 tcaaattgag aaaaccctaa gctaacagaa gaaaacaaag ctttaagtgt ttctaatgcc
     1321 tttgaagtta aatttacttt ttttgccatt tcttttaatt tttattatta gtgttcctat
     1381 attatgttaa tgtatatttc agttacctaa gaagttattt aagaacttat tcatctcttt
     1441 taccttagta gccataggtc tatcctgctt actaaaagct tctttaaatt ctttaatagc
     1501 aaacaaccct tctacactaa atccatttct cttaccttct ttaatatcct cccaaagctc
     1561 tttagaattt actttagctc cgattatcca gcttccatct ggagtatctt ctaagccctt
     1621 aggagcatta attcctttct cggtatctac tatataagat tggtatataa agctgtctgc
     1681 gtcctcgtct gtatgctcta cgttcatctt agtattataa cctttcttaa agaggtttaa
     1741 ggcaatctgt ttaatagttt ctttactgaa tactacataa aactgcttac cgtctatctc
     1801 tcggtatata ggtttgtctg
//
LOCUS       DATGSI010000457         1041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_221784, whole genome shotgun sequence.
ACCESSION   DATGSI010000457 DATGSI010000000
VERSION     DATGSI010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221784"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..245)
                     /locus_tag="VK031_07075"
     CDS             complement(15..245)
                     /locus_tag="VK031_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53718.1"
                     /translation="MEIKAGDRFKVSLGIEATEIGSSTISLDNQTVTVVEDGDTMGSY
                     YIVKIKDDRLGRLVDKYKFDKGIKRNRILKIN"
     gene            complement(346..>1041)
                     /locus_tag="VK031_07080"
     CDS             complement(346..>1041)
                     /locus_tag="VK031_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53719.1"
                     /translation="GIGKTTVNKRVNFLLSIGLMELSEEGHLKHLSKKELVDWCSDYL
                     GKDLGYGYITIKLHNKVKHTKYNIYTRVSLNGIRQQRYNARRRAEVNDIGVRVSKGLI
                     VSKKELDLYNRHKETLRQNTENAVKFSGEKVNYMSDSWISGKTGLSISTVRAMVDFWV
                     SQGLLKTTFVKGRVLDKRVTPKSYQALLDTRYNEFRSTYLYKGNIVEYNKRAVDYGDS
                     IWADEETPIVSNK"
BASE COUNT          298 a          211 c          118 g          414 t
ORIGIN      
        1 gtatgttgga acgtttagtt tatcttaaga attctgtttc tttttatacc tttgtcaaac
       61 ttgtatttat ctacaagcct tcctaatcta tcatctttta tcttaactat atagtaagat
      121 cccatagtat ccccgtcttc aactaccgtt accgtttgat tatccaaact tattgtacta
      181 gatcctattt ctgtagcttc tatacctaaa ctaaccttaa acctatctcc tgctttaatt
      241 tccatagtca ttttgtttta atcatcattc aatgatacaa acataaacat tgtatttgac
      301 ttaaacaaat attttacata ttattttaat atttaaggtg tccgcttatt tattagatac
      361 tattggggtt tcctcatctg cccatatact atctccataa tcaacagccc tcttattata
      421 ctctactata ttacctttat ataagtaagt acttctaaac tcattgtatc tagtgtctaa
      481 tagtgcctga taagactttg gagtaactct tttgtctaaa actcttcctt ttacgaaagt
      541 agtctttaat agtccttggc taacccaaaa atctaccata gctctaacag tagatattga
      601 taaacccgtt tttccagaaa tccaagagtc tgacatgtag ttaacttttt ctccgctaaa
      661 ctttactgcg ttttcagtgt tttgtctgag agtttctttg tgcctattat atagatctaa
      721 ttctttctta gatactataa gacctttaga tacccttacc ccaatatcat tcacttccgc
      781 tcttcgtctt gcgttgtaac gttgctgtct tatcccgttt aaagaaacac gagtataaat
      841 attatatttt gtatgcttta ctttattatg aagttttata gttatataac cataacccaa
      901 gtctttacct aggtaatcag aacaccagtc cactaattct ttcttagata gatgttttag
      961 atgaccctcc tctgataact ccattaatcc tatagataat aggaaattaa ctcttttatt
     1021 tacagtggtt tttccaatac c
//
LOCUS       DATGSI010000458         3406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_313113, whole genome shotgun sequence.
ACCESSION   DATGSI010000458 DATGSI010000000
VERSION     DATGSI010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313113"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VK031_07085"
     CDS             complement(<1..489)
                     /locus_tag="VK031_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLS53720.1"
                     /translation="MVVLDLVFKLSIIIFVGIIGGRIASYFKLPNVSGYIVGGLLLGP
                     SVFNIIKSGEAANFNIVNEVALAAIAFSVGSEFLFKDIVKVGKDMLIITVTEVAGALL
                     FVFVTTYFIFRQPFEFSLVIASMSAATAPAGILMVIRELRAQGPLVRTILPVVALDDA
                     LGI"
     misc_binding    715..916
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1060..2151
                     /locus_tag="VK031_07090"
     CDS             1060..2151
                     /locus_tag="VK031_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HLS53721.1"
                     /translation="MSKKLFIPGPVSVRSDVLAQMTKPIIGHRTEEASHLQRGISKKM
                     QKVFYTKNKILLSTSSGTAMMEGAIKSFTKDRAAIFSNGFFGDLWHELALRNNIEADL
                     YRPKEGEAIDPNMVDKVLSTGRYDLVAITHNETSSGITNPMEELSKVIKNYPDLIWCV
                     DGVSSVGGIKLEVDKLGIDVCIVSSQKCLGLPPGMAIASVSKKAIERARSIECRGYYL
                     DFLELYETIDREDYQYISTPAIPLMYAMDYQLDKILEEGLENRFSRHKELGRLTRNFI
                     KKYFDLYPKEYCASNTVTTIKNTRNIDVDFLNTKLGEMGFQISNGYGYLKDKTFRIGH
                     MADCTIEELQELLDCLEEIFTSWDYGKVV"
     gene            complement(2287..3405)
                     /locus_tag="VK031_07095"
     CDS             complement(2287..3405)
                     /locus_tag="VK031_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HLS53722.1"
                     /translation="MGVGLGLVANESVIGVIQSIKHVVGQFIFFCVPLVIIGFIAPSI
                     TGLKDNASRMLGGALTISYLSSVGAALFATVAGYLIIPGLRFATDVADTKELPEMIFQ
                     LNIDPVMTVMTALVLSIVIGLTVIWTKSKTFETLLQEFNNIMLSVVNNMVIPILPVFI
                     ATTFAVLAYEGAIVHQLPVFISIILIVIVGHFIWMGILYAIAGIISGENPKEVFKHYL
                     PAYLTAIGTMSSAATLPVALESARKSTVLDDEVVDFAIPICSTIHLCGSVLTETFFVM
                     AISQILYGELPAFGSMVLFVVLLGIFAVGAPGVPGGTVMASLGIVYAVLGFDDAGVGL
                     LLSIFALQDSFGTACNIVGDGAIALMLTGLYKKDKKSA"
BASE COUNT         1160 a          607 c          610 g         1029 t
ORIGIN      
        1 tatacctagg gcatcatcta aggctaccac tggtaaaatg gtcctaacca atggcccctg
       61 tgctctcagt tctctaatta ccattaatat tcctgcaggt gccgtagctg ctgacatgct
      121 agcaataact aaactaaatt caaaaggttg cctaaatata aaataagttg tcacaaaaac
      181 aaataaaagg gcacctgcaa cttcagtcac cgttattatc aacatatcct ttcctacttt
      241 tacaatatct ttaaataaga attcactacc tacactaaag gcaattgctg ctaaggccac
      301 ctcatttaca atgttaaaat tagctgcctc tccagactta attatattaa aaactgatgg
      361 acctaacaat aagccaccta ctatataacc cgatacattg ggtaatttaa agtagctggc
      421 tattcttcct ccaataatac ctacaaaaat tattatactt agtttaaaaa ccaagtccaa
      481 tactaccatg cctccctttt catcatcttt ttccagttag ctactgagct attataaacc
      541 ataaatggat aataatcaag taaggcctgt atacatcaag gatagaagag atttaatttt
      601 aacttcatat gtttagaagt ttttaaaaga aatcctaatt tatataaaga agcaattttt
      661 tgaaaattaa cccttgcatt ttaaaaaggc ttattatata ataagcttta atacattaaa
      721 ctgacgatga ggacaatgat aaaggcctta attccagaga gcctacggtt ggtgtaagta
      781 ggtattaagt tttatcatcg ctacctctga gggtttgcct aataagcaaa acgggattac
      841 agccgttata ctgttggaga gcggatcatt agatcaattt gggtggtaac acggaagtca
      901 gcctttcgtc ccttacaggg atgaagggct tttttaatga aagctttagt ttttgcaacc
      961 caaatttctc tgatcaatct gggtggtaac gcggaagcta gcctttcgtc ccttacaggg
     1021 atgaagggct ttttttatta aattataagg aggcaaaata tgagtaaaaa attatttatt
     1081 ccaggaccag taagtgtaag gagtgatgta ttagcacaaa tgacaaaacc tattatagga
     1141 cataggactg aagaggcctc ccatttacaa agaggcataa gtaaaaagat gcaaaaagtt
     1201 ttttatacta aaaataaaat attattgtcc acttcatccg gtacagccat gatggaagga
     1261 gctataaaat cttttaccaa ggacagggct gccatatttt ccaatggttt ttttggagat
     1321 ctctggcatg aattggccct aagaaacaat attgaggcag atttatatag gcctaaggaa
     1381 ggagaggcca ttgatcctaa catggtagat aaggtacttt ctactggtag gtatgatctt
     1441 gtagccataa cccacaatga aacttcatct ggaataacta atcctatgga ggagctttct
     1501 aaggtgatta aaaactatcc tgatcttatt tggtgtgttg acggtgtaag ctctgttggt
     1561 ggtattaagt tagaagtaga taaattaggt atagatgtct gcattgtatc cagccaaaaa
     1621 tgtttaggac ttcctccggg aatggcaata gcctcagtat ccaaaaaggc tatagaaagg
     1681 gccagaagta tagagtgtag agggtattat ctggactttc ttgaattata cgagactata
     1741 gatagggagg attatcagta tatatctact ccggccattc ctctaatgta tgctatggat
     1801 tatcagttag ataagatatt agaggaaggc ttagaaaaca ggtttagtag gcacaaggaa
     1861 ctaggaaggc ttaccagaaa ctttataaaa aaatactttg atctctaccc taaagaatat
     1921 tgcgcctcta atacagtgac cacaataaaa aacactagaa atatagatgt tgattttctt
     1981 aatacaaaat taggtgaaat gggctttcag atctccaatg gatatggata tttaaaagac
     2041 aaaaccttta gaatagggca catggcagat tgcaccattg aagagttgca ggagctatta
     2101 gattgtcttg aagaaatttt tacctcatgg gactatggta aggtggtgta gctaaggtgc
     2161 agctaaggtg tagctaaggt gcagctagac tcaatatgat gttagtgcaa gtttaaatta
     2221 attaaataaa gcataaatct agtaaataaa aaaaccgccg taaggcggtt ttttaaatat
     2281 taaacattat gcagattttt tgtctttttt ataaagacct gttaacatta aagctattgc
     2341 tccatctcct actatattac atgcagttcc gaagctatct tgtaaggcaa atattgataa
     2401 cagtagtcca actcctgcat catcaaatcc aagtactgca taaactatac ctagagaagc
     2461 cattacagta cctcctggta ccccaggtgc tcctactgca aatataccta ataaaactac
     2521 gaataatacc atgcttccaa aagcaggtaa ttctccatat aatatttgag atatggccat
     2581 tacgaaaaat gtttctgtaa gtactgaacc acacaggtga attgtagaac aaatcggtat
     2641 agcaaagtct acaacttcat catctagcac agttgatttc cttgctgatt ctaaggctac
     2701 tggtaaagta gcagctgatg acatggttcc tatagctgtt aagtaagctg gtaagtagtg
     2761 tttaaatact tcctttggat tttctccaga tattatccca gcaattgcgt atagaatacc
     2821 catccatata aagtgtccaa ctataacaat taatataata ctgataaata caggtaattg
     2881 atgaacaatt gctccttcat aggctaaaac tgcaaatgtt gtagctatga atacgggcaa
     2941 tataggtata accatattgt ttactactga tagcataatg ttgttaaact cctgtaagag
     3001 agtttcaaag gttttagatt tagtccaaat aacagtcaat cctataacta ttgaaagaac
     3061 tagtgcagtc ataactgtca ttactggatc aatatttaat tggaaaatca tttcaggtaa
     3121 ttccttagta tctgccacat ctgtagcaaa tcttaatcct ggtataatta aatacccagc
     3181 caccgttgca aatagggctg ccccaactga tgataagtag gatattgtta atgctccacc
     3241 tagcattcta ctggcattat cttttaaacc agtaattgat ggtgctataa aaccaatgat
     3301 tactagtggc acacagaaga atataaattg tcccactacg tgcttaatgg attggataac
     3361 cccgattaca ctttcattgg caactaagcc taagcctaca ccgata
//
LOCUS       DATGSI010000459         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_361856, whole genome shotgun sequence.
ACCESSION   DATGSI010000459 DATGSI010000000
VERSION     DATGSI010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361856"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1080)
                     /gene="glyS"
                     /locus_tag="VK031_07100"
     CDS             complement(<1..>1080)
                     /gene="glyS"
                     /locus_tag="VK031_07100"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HLS53723.1"
                     /translation="GACRQIKEKFEGIFEEHNINYEKIQLYSTPRRLTTIISGLDERQ
                     KDKEENIKGPSKRISFDEDGEPTKALLGFMRGQKVELEDIIFMEHNGEDYVYAQVLKK
                     GKSIEEILEESVPKLIRTINFPKSMKWGGKNLRFARPIRWIVSLLDDQVLSFDLEGIK
                     VSNATRGHRFLGSSNLIINNIDEYEKVLRENYVLIDPQERKEKIQYASERLAREKGGR
                     LQCDEDLLDEVTHLVEYPTPIIGRIKEEYLSLPMDVVITPMKEHLRYFPVLDSKGRLL
                     PYFITVRNGGEDYIDVVVKGNEKVLGARLEDARFFFNEDISRPLDSYVDELKDIIFQE
                     KLGTVYDKTVRIKKLAKRIAGYLEVG"
BASE COUNT          288 a          250 c          138 g          404 t
ORIGIN      
        1 tccaacctcc aggtagccag caattctttt tgccaatttt ttaatcctta cggtcttatc
       61 ataaacagta cctagttttt cttggaaaat tatatctttt aattcatcca cataactatc
      121 taagggtcta cttatatctt cattaaagaa aaatctagcg tcctccaatc tagctcccaa
      181 aactttttca ttacccttta ccaccacatc tatgtagtcc tctccaccat ttcggacagt
      241 aataaaatat ggtagcaatc tacccttact atctaaaact ggaaaatatc ttaggtgctc
      301 cttcattggt gttattacca catccattgg caggcttaaa tattcttcct taatccttcc
      361 tataatgggt gttgggtatt caaccaggtg ggttacctca tctaatagat cttcatcaca
      421 ttgtaacctg ccaccctttt ccctggccag cctttcacta gcgtattgga ttttttcctt
      481 cctttcctga ggatcaatta gcacataatt ttctcttaat accttttcat attcatctat
      541 gttattaata attagattgc tacttcctaa aaatctgtga cctctggttg cattggaaac
      601 ctttattccc tccaggtcaa aactaagaac ctgatcatct aatagagata caatccatct
      661 aatgggtctt gcaaatctta aatttttacc tccccacttc atagacttag gaaaatttat
      721 ggtcctaatt agttttggca cactttcttc taagatttct tctatagatt tacccttttt
      781 caaaacctgg gcataaacat aatcctcacc attatgttcc ataaaaatta tatcttctag
      841 ttctactttt tgccctctca taaagcctaa aagagccttg gtcggttctc catcctcatc
      901 gaaggaaatc cttttggagg gtccctttat attttcttcc ttgtcttttt gtctttcatc
      961 caggccagaa attatggttg taagccttct aggagttgaa taaagttgaa ttttttcata
     1021 gtttatattg tgttcttcga aaattccttc aaatttttcc tttatctgtc tgcaggcccc
//
LOCUS       DATGSI010000460         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_504013, whole genome shotgun sequence.
ACCESSION   DATGSI010000460 DATGSI010000000
VERSION     DATGSI010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504013"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /gene="tyrS"
                     /locus_tag="VK031_07105"
     CDS             <1..583
                     /gene="tyrS"
                     /locus_tag="VK031_07105"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HLS53724.1"
                     /translation="TFQLLTTASGTKMGKTESGTIWLDPEKTPPYELYQYLRNVDDRD
                     VEKFLKLLTFIPMDEIQELASLEGSEINKAKEILAFEVTKTIHGEGEAVKAQNAARAL
                     FAGEQKEGAIPFTEIDKRQLEAGMGIIELLRKAELIKSNSEGRRLINQGGISIDDERV
                     QSIHRQVTLDEFVDGKLLLRKGRKVYHQIRVKP"
     gene            903..1637
                     /locus_tag="VK031_07110"
     CDS             903..1637
                     /locus_tag="VK031_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HLS53725.1"
                     /translation="MFRKNRVLVLSIITLLLLGTVGQAAGFDYRGHNPKSYNVNYSFI
                     DQYTNVSYNYNWVVTNFTSRPSQAPKPSLPMVPERPEKPENTDRPERPNRPENPGKPV
                     LPEEVGKPELPNTSTLSQVEAEVVRLVNIERQKQGLAPFKVSSELSNVARVKSQDMAQ
                     NNYFSHTSPKYGSPFDMMRSFGIKYTTAGENIAKGYLTAQSVVNGWMNSSGHRANILN
                     PSFNTIGVGAATDARGHIYWTQMFTN"
     gene            complement(1693..2313)
                     /locus_tag="VK031_07115"
     CDS             complement(1693..2313)
                     /locus_tag="VK031_07115"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HLS53726.1"
                     /translation="MDITYLTRLLKNRVPRSIEPMNKFAVLLPLIWEKGEWRLIFEKR
                     AKDLNSQPGEISFPGGGLESDESFLEAAVRETMEELNLEKDNIEVIGELDYLISYAAL
                     EIHCFLGTIRGIDVDKIEPNPAEVDHIFTVPLGYFLENQPKEYYLDLVTRYNEEFPYN
                     LIPKGKDYDFRPVRRNIYFYSYKDYIIWGYTASMIKNLVDIIKGKK"
     gene            complement(2337..3548)
                     /locus_tag="VK031_07120"
     CDS             complement(2337..3548)
                     /locus_tag="VK031_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HLS53727.1"
                     /translation="MYGLVLEGGGAKGSYHIGAYKAILEEGIEIKGIAGTSIGALNGA
                     MIVQGDFEICYKLWEELSYSMIIGEKDKEIEKLLQFEPSVENFKLAVQKTFEIITGKG
                     ISTEPFRKLLKTYIDEERIRQSNMDFGIVTVNLSDLKPEEIMLEDIPEGKLIDYLMAS
                     AYLPVFKIERLGGKIYLDGGFYDNLPFRILLNKGYKDLILVRTHGRGLTRKIDTNKIN
                     ATIISPSDDIGRSYTYEANIAKRNIRLGYFDGLKALRGLLGNKYYIDDKNCKDFALDF
                     LLSIEDEKIDKIVDLMKGPEVKGKRALFEYIIPKLGSIMGLKDDFSYQEFLVELLEKR
                     ALKSQVERFKIYSIKEFFHILKNKVKESEYKKMEERSSLEKVIEKVDIALLFNKEDLL
                     LEITDILLKED"
     gene            complement(3572..>3846)
                     /locus_tag="VK031_07125"
     CDS             complement(3572..>3846)
                     /locus_tag="VK031_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HLS53728.1"
                     /translation="DGTTYQTLAEAQEAGAITLNYGVFIGAVVDFIIIAFSIFFVIRQ
                     INKLRPEEVVEEEPTTQECPYCITEISVKASRCPNCTSSLEGKLAN"
BASE COUNT         1202 a          765 c          591 g         1288 t
ORIGIN      
        1 gaccttccaa cttttaacta ctgccagtgg aacgaagatg ggtaaaaccg aatctggtac
       61 tatttggtta gatcctgaaa aaactccgcc atatgaatta tatcagtact tgagaaatgt
      121 ggatgataga gatgtggaga agtttttaaa actattgacc tttataccta tggatgagat
      181 ccaggagtta gcatctcttg aaggctcaga aataaataag gctaaagaaa tcttagcctt
      241 tgaggttacc aaaaccattc atggagaagg tgaagcagtt aaagcccaaa atgctgctag
      301 agcactattt gcaggagaac aaaaagaagg ggccatacct tttactgaaa tagacaaaag
      361 acagcttgag gcaggcatgg gcataataga acttctaaga aaggcagaac ttattaaatc
      421 taatagtgaa ggtagacgat taataaatca aggtggcatt tccatagacg atgaaagagt
      481 tcagtctatc catagacaag ttaccttaga tgagtttgta gatgggaaac ttttactgag
      541 aaaaggcaga aaagtatacc atcaaattag agtaaaaccc tagcagtgct agggttttac
      601 ttttctccct tttaaggccc cttccatagc ttatttctaa ccttatacaa tttctttcct
      661 ttccaccaca gttgtagaaa atcctatcta gcttcattta cttttatttt cttttaaacc
      721 atatgtttca tactagaatt ttcaagtgtc cttatgctgg cttaaaaata tctccatata
      781 agtggattag ataacaaatt tatgtaattt aaccaaaaac tgtgccttta ataggcttag
      841 ggctcatgct atacttatta aaagttacaa aattattaca aaacaaattt aaggaggatt
      901 ttatgtttag aaaaaataga gttttagttt tatcaataat taccctatta ttgttaggga
      961 cagtaggaca agcagctggg tttgattata gaggtcataa tccaaagagc tataatgtta
     1021 attacagttt tatagatcag tatacaaatg taagctataa ctataattgg gtggtgacta
     1081 actttacaag taggcctagt caggcaccaa agccttccct acctatggtt ccagaaaggc
     1141 ctgagaaacc tgaaaatact gacaggcctg aaagacctaa taggccggaa aatccaggta
     1201 aacctgtact gcctgaggaa gttgggaaac ctgaattacc taatacttcc accttatcac
     1261 aggtagaggc agaggttgtt agactagtta atatagaaag acaaaaacaa ggtttagctc
     1321 cttttaaggt cagttcagag ctatctaatg ttgcaagggt taagtctcaa gatatggcac
     1381 aaaataacta ctttagccat acttcaccta aatatggtag cccctttgat atgatgaggt
     1441 cctttggtat taagtacact acagcaggtg aaaatattgc taaaggttac ttaactgccc
     1501 aatcagttgt aaatggctgg atgaattcct caggccatag ggcaaatatc ttaaatccca
     1561 gctttaacac cataggcgtc ggcgcagcca ctgatgccag gggccatatc tactggacac
     1621 aaatgtttac taattaaaaa tttaaaaagt agatagggga gactccctat ctactttttt
     1681 ctatctttat ctttattttt tcccctttat tatatccact aaatttttaa tcatactggc
     1741 tgtataaccc catataatat aatccttgta actataaaaa tatatatttc ttcttactgg
     1801 tctaaagtca taatcctttc ccttaggtat caaattatag ggaaactctt cattgtacct
     1861 ggttaccaaa tctagatagt attcttttgg ttgattttct aaaaaatatc ctaaaggaac
     1921 tgtgaatatg tggtccactt ctgcagggtt aggttctatt ttatccacat ctattcctct
     1981 aattgttcct aaaaagcaat ggatttctaa agctgcatag gaaattaaat aatctaactc
     2041 tcctataacc tctatgttat ccttttctaa gtttaactct tccattgtct ccctaactgc
     2101 agcctctaaa aaactttcat cactttccaa gccaccgcca ggaaaggata tttctcctgg
     2161 ctgagaattt aaatccttag cccttttttc aaatataagc ctccattcac ccttttccca
     2221 aatcaaaggt aacaaaacag caaatttgtt cattggctct atggatctag gcactctgtt
     2281 ttttaatagc cgggttaaat aggttatatc caactgctcc accccttttg taaatcttaa
     2341 tcctctttta ataagatatc tgttatttct aaaagcaagt cttctttatt aaataatagg
     2401 gctatgtcca ccttttcaat aaccttttcc aaagagcttc tttcctccat ctttttatat
     2461 tcactttcct taaccttatt ctttaaaata tggaaaaact ctttaatact atatatttta
     2521 aatctttcta cctgggattt aagtgctctt ttttccaata actccactaa aaattcctgg
     2581 tagctaaaat catccttaag ccccataatg gaacctagtt tagggattat atattcaaat
     2641 aaggctcttt tccctttaac ttctggtccc ttcattaagt caacaatctt atcaattttt
     2701 tcatcttcta tagataatag aaagtctaag gcaaaatcct tacagttttt atcgtctata
     2761 taatacttat tgcccaatag gccccttaag gcctttaatc catcaaaata tcctagcctg
     2821 atatttcgtt ttgctatatt ggcctcatag gtataggacc ttccaatatc atctgacgga
     2881 gatattatgg tggcattgat tttattagta tcaatttttc tagtgagacc cctgccgtgg
     2941 gtccttacca gaattaaatc cttatatccc ttatttaata ggattcgaaa aggtagatta
     3001 tcataaaaac caccgtctaa atatattttt cctcccaatc tttctatttt aaatactggc
     3061 aaataagcag aagccatcaa ataatcaatt aatttacctt caggaatatc ttccaacata
     3121 atctcttctg gttttagatc tgataggttt acagttacta tgccaaaatc catatttgat
     3181 tgccttattc tttcttcgtc tatataggtt tttagtaatt tccgaaatgg ttccgtacta
     3241 attcccttgc ccgttattat ctcaaaggtt ttttgtaccg ccagtttaaa gttttctaca
     3301 ctaggttcaa actggagaag tttttctatt tccttatcct tctcaccaat tatcatggaa
     3361 taagataact cttcccagag cttatagcaa atctcaaagt ctccttgtac tatcatggca
     3421 ccgtttaaag cacctatgga tgttcctgca atacctttta tttctattcc ttcctccaaa
     3481 atcgccttat aggctcctat gtgataggag cccttagcac ctccaccttc taatactaaa
     3541 ccatacattt ttaccacccc ggtgagaaat tttaatttgc cagtttcccc tctaaactag
     3601 aggtacaatt tggacatcta ctggccttta cagatatttc tgtaatacaa taagggcatt
     3661 cttgagttgt aggttcttct tctaccactt cttctggtct tagcttgttt atctgtctaa
     3721 taacaaagaa tatggaaaag gcaataatta taaaatcaac aaccgcccca ataaacactc
     3781 cataatttaa ggttatggcc cctgcttcct gggcctctgc taaagtttgg taggtagtcc
     3841 catcta
//
LOCUS       DATGSI010000461         1852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_313373, whole genome shotgun sequence.
ACCESSION   DATGSI010000461 DATGSI010000000
VERSION     DATGSI010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313373"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VK031_07130"
     CDS             <1..883
                     /locus_tag="VK031_07130"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HLS53729.1"
                     /translation="NFNPDIVIGTGGYVCGPVVFQATRRGIPTLIHEQNAFPGVTNKI
                     LSRYVDKVAITFKESVEYFKHPEKTILTGNPIRDELFQINRELAYKELKVDPKTPLIL
                     SFGGSGGQKALNDAILGIIKNNLIYNDFQLIHITGHRLYDDFVEQIEKLNIKLGDNIK
                     ILPYLYEMPAALNIADLIISSAGAITLAEISAVGIPSILIPKGYTAENHQEYNARAFE
                     EAGASLVILEKDLEERRLGEKINKLLFDQDLLNRMAVKSKKIGKINAAEEIYLTYFLG
                     KINAAEEIYEIISQLIS"
     gene            953..>1852
                     /locus_tag="VK031_07135"
     CDS             953..>1852
                     /locus_tag="VK031_07135"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HLS53730.1"
                     /translation="MAKYIIQGGNRLEGEVEIKGAKNSILPILAATVISGEVSKISNI
                     PQLRDVEVMTKILLSIGAKVEKEGDQLLIDTSSIDKTQLPEELVREMRSSIILMGALL
                     ARFGEVSVSYPGGCEIGPRPIDLHLKSLRQMGAIIEESHGFLHCRSLGLQGADIQLDY
                     PSVGATENIMLAASIAEGRTIIRNAAREPEIIDLQDYLRKAGAKIKGAGTSVITVEGV
                     PSLKGCSHRIIPDRIVAGTLMVAATITKGEMVVKDIVVEHLQSTVAKLRETGANIFIN
                     GNAIKLIGPERVEPIELLQTLPYP"
BASE COUNT          648 a          292 c          404 g          508 t
ORIGIN      
        1 aaactttaac ccagatattg tcataggaac aggaggctat gtttgtggac ctgtggtttt
       61 tcaagctaca aggaggggca ttcccaccct tatccacgaa caaaatgcct ttcccggtgt
      121 aaccaacaaa attttgtccc gctatgtgga caaggtggcc ataacattta aggaatctgt
      181 agaatatttt aaacacccag aaaagactat ccttactggc aatcccataa gggatgaatt
      241 atttcaaata aatagggaac tggcatataa agaactaaag gtagacccta aaacacctct
      301 tatcctatcc tttggtggca gtggggggca gaaggcctta aatgatgcaa tccttggaat
      361 tataaaaaac aaccttatat ataatgactt tcaactaatt catattacag gccataggct
      421 ttatgatgat tttgtggagc aaatagaaaa acttaatata aagctgggag acaatataaa
      481 aatacttcct tatctatatg aaatgcctgc agccttaaat attgcagact tgattatatc
      541 tagtgctggg gctattacct tggctgaaat atcggcagta ggaattccta gtatcctgat
      601 tcctaaagga tatactgctg agaatcatca agagtataat gcaagggcct ttgaggaagc
      661 cggagccagc ctagtaattt tagaaaaaga tttagaagaa agaaggctgg gagaaaaaat
      721 taacaaactt ttatttgatc aagacctact gaataggatg gctgtaaaga gcaagaaaat
      781 aggtaagata aatgcagcag aagaaattta tcttacctat tttctaggta agataaatgc
      841 agcagaagaa atttatgaaa ttatttccca attaatatca taagagtaac cgcctccaaa
      901 tttatgcata agataaaaat aaatagaatc taatgcattt ggaggtggaa taatggctaa
      961 gtacattatt caaggtggga atagattgga aggggaagta gaaataaagg gagctaaaaa
     1021 ttcaattctt cctatcttgg ctgcaacagt tatcagtgga gaggtaagca agatatcaaa
     1081 tatacctcag ttacgagatg tggaagtgat gactaagatt ttattatcta ttggggccaa
     1141 ggtggaaaag gagggggacc aattattaat agatacttcc tccatagata aaacccaact
     1201 tccagaagaa ttggtaagag aaatgcgctc atctattata ttaatggggg ctctgttggc
     1261 caggtttggt gaggtcagtg ttagttatcc aggaggctgt gaaatagggc caagacctat
     1321 agacctacat ttaaaatcct tgagacaaat gggggcaatt atagaagagt ctcatggttt
     1381 tctccattgc aggagtcttg gtcttcaagg agctgatatt cagttggatt atccttctgt
     1441 tggggccaca gaaaatataa tgttggctgc ttccatagct gaaggtagaa ctattattag
     1501 aaatgcggcc agagaaccag aaattataga tctacaggat tatttaagaa aggccggtgc
     1561 taaaataaaa ggcgcaggca ccagtgtaat aacagttgaa ggagttccat ctttaaaggg
     1621 ttgttcccat aggataattc ctgataggat agtggctggt accttaatgg tggcagcaac
     1681 aataactaag ggtgagatgg tggttaaaga tattgtagta gagcatctac aaagcacagt
     1741 tgctaaatta agggaaactg gtgccaatat atttataaat ggaaatgcta tcaagcttat
     1801 aggaccagaa agagttgagc ctatagaatt attacaaact ttaccatatc ca
//
LOCUS       DATGSI010000462         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_647241, whole genome shotgun sequence.
ACCESSION   DATGSI010000462 DATGSI010000000
VERSION     DATGSI010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647241"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1005)
                     /gene="fliM"
                     /locus_tag="VK031_07140"
     CDS             complement(16..1005)
                     /gene="fliM"
                     /locus_tag="VK031_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HLS53731.1"
                     /translation="MKQVLSQQEIDALLNALSTGEIDPETIEEETNKVRSYDFRRPIK
                     LSKEYINTIQMIFENFSKIADNLLSTQLRTNVDINLGSLEQISYDEFIRSIPRFTLAA
                     LFTSDNLGGTQIMEINPQFCLQVIELVCGGSPSDYTEKLSKKDSFTDIELGILEELVR
                     NLLTAFESAWSEILDIKTNINSIETNPQLTQTMSPNEPVVLASFTVEIFETRSFINIC
                     IPYISFEQILDKLSFKSWFDFEKEVDEDNREVLEKRVKSVAVDLEVLIGKAYLTVSDF
                     LQLEKGDVFQLDLKTTDPLKLYIEGKPYYLVRPGKVNDKYGVEILQYIEEGVD"
     gene            complement(1008..1412)
                     /locus_tag="VK031_07145"
     CDS             complement(1008..1412)
                     /locus_tag="VK031_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HLS53732.1"
                     /translation="MQIIIFTIGDKYYGLNTEEVEEIIDETQSKKVPNSPDWVEGLIN
                     LRGSVVTTINLSKLLRQKEDLCYNNIIIINDNEEKIGLLIKDVVEVIDIDEKDIEQID
                     MEAEDAIIGILRVNDRLVNIIDINILFSKNEG"
     gene            complement(1425..1787)
                     /locus_tag="VK031_07150"
     CDS             complement(1425..1787)
                     /locus_tag="VK031_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLS53733.1"
                     /translation="MSKRVLIVDDAVFMRMKLKDILEKHGYEVVAEAANGIEAMEKYK
                     QEKPDLVTMDITMPDMDGITALKGIKEVDPDAKIIMCSAMGQQTMVMEAIQAGALDFI
                     VKPFDTDRVIQSLDRISG"
     gene            complement(1801..2400)
                     /locus_tag="VK031_07155"
     CDS             complement(1801..2400)
                     /locus_tag="VK031_07155"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HLS53734.1"
                     /translation="MNKGEFTPLQIDLIKELANIGGGNAATSLSQLINKPTSMTVPTI
                     EILNYNEVYKEIMAEETIVDAVLMKMLGDAEGLFLFVNSEESSQHLVEMMVDENIELT
                     EQIYISAIKELVNIIVSSFLNAVSKMVDVSLISSVPVHVKDMFGAILSSVYIETGQYD
                     ENIMIIKNEFLYLGEKIESSLYFVPKPGVLKSLFQIIGV"
     gene            complement(2393..>3902)
                     /locus_tag="VK031_07160"
     CDS             complement(2393..>3902)
                     /locus_tag="VK031_07160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HLS53735.1"
                     /translation="GAYERGYKAYSILVKVEDYCSLKGARAYLVVNKLEQQGDVIKTN
                     PNPEELEEGNYDKIFMLVYLSKLDKAAIKDMIEGIAEIESVLVKEMEDTTSKVDEKAA
                     TKEKPASNKRRDRKVHQMNQSIRVDLGRLDRFMNLVSELVIYRTRLEDLSNNYDIPEI
                     HEPLEYVGRITSELQDLVLKIRMQPVNVVLNRFPRMVRDLSQELNKEIEFVIEGEDTE
                     LDRTIVSELGEPLVHLIRNAADHGIESVEERRALGKPDQGLIKLNAYQEGNRVVIIVS
                     DDGKGIDPDKIKQSAEEKGIDTEGMSQREIIQLIFDQGFSTVKDVTNVSGRGVGMDVV
                     RQKISSLGGNIEVISEVDKGTSFIIRLPLTLSIIQTLMVYIGEEVFAIPLGIIERVVK
                     IRPEEIVESHNSEIYIYMDRAVPVVRLNEKLNLESSSSDNHLILIQLGNQYYGLLVDE
                     LIGQQETVIKKLSGPLSKMKEYLGATILGNGEIVLILDVGNLCDRTEGGLIE"
BASE COUNT         1069 a          860 c          549 g         1424 t
ORIGIN      
        1 aaaagtcttt attcattagt caacaccttc ttctatatat tgtaatattt ctaccccata
       61 tttatcattt actttccctg gccgtaccag atagtagggt ttgccctcta tatatagttt
      121 tagaggatct gtggtcttta aatctagctg gaaaacatct cccttttcca gttgtagaaa
      181 gtcactaaca gttaaatatg ccttaccaat tagaacctct aaatctactg ctacagactt
      241 gactcttttt tccaatacct ccctattgtc ctcatccact tctttttcaa agtcaaacca
      301 acttttgaag cttagcttgt ccagtatttg ctcaaaggag atgtagggaa tgcatatatt
      361 tataaaggat ctggtttcaa aaatctccac cgtaaaactg gccaatacca ccggttcatt
      421 aggtgacatg gtttgggtta gttgtggatt agtttctatt gaattaatat tagtctttat
      481 atctaaaatc tcgctccagg ctgattcaaa ggctgtaagc aagtttctaa ctaactcctc
      541 taaaatcccc agctctatat ctgtaaagct atccttcttg gaaagttttt ctgtatagtc
      601 actaggtgag ccaccacaaa ccaactctat aacctgtagg caaaattgag ggtttatttc
      661 cattatttga gttcctccca ggttatcaga ggtaaatagg gctgccaagg taaatctagg
      721 aatggagcgg ataaattcat cataactaat ctgttccagg gaacctagat ttatatccac
      781 attggtccta agttgggtag acaaaagatt atctgcgatt ttagaaaagt tttcaaatat
      841 catttggatt gtatttatat attccttaga aagttttatg ggccttctaa aatcgtagct
      901 tcttaccttg ttagtctcct cttcaattgt ttcagggtca atttcacctg tactaagcgc
      961 atttaaaagc gcatctattt cttgttgtga taatacttgt ttcaagttta tccctcattt
     1021 ttcgaaaata atatatttat atcaatgata ttcactaatc tatcatttac tcttaaaata
     1081 cctataatag catcttctgc ttccatatct atttgttcta tatccttctc atctatatct
     1141 ataacctcta ctacatcctt aattaaaagt cctattttct cctcattatc attgattata
     1201 ataatattat tataacataa atcctccttc tggcgaagga gcttagatag gtttatagtg
     1261 gtcaccacgc tacctcttag gttgatcaat ccttcaaccc agtctggaga attgggtact
     1321 tttttagact gtgtttcatc gattatttct tctacttctt ctgtatttaa gccataatat
     1381 ttatccccaa tggtaaatat tataatttgc ataatttcac ccctttatcc actaatccgg
     1441 tctaaggact gaataaccct gtctgtatca aagggcttga ctataaagtc taaggcaccg
     1501 gcttgaatag cctccattac catggtttgt tgccccatgg cactgcacat aattattttt
     1561 gcatcaggat ctacttcctt aatgcctttt aaggcagtaa tcccatccat atccggcata
     1621 gtaatatcca tggttaccag atcaggtttt tcttgtttat atttttccat ggcctcaata
     1681 ccattggcag cctcagccac tacttcataa ccatgttttt ctaatatatc ttttagtttc
     1741 attctcataa aaacagcatc gtctacaatt aaaactctct ttgacatttt catatacctc
     1801 ctaaactcca atgatttgaa ataaagattt taatactcct ggttttggaa caaaatatag
     1861 ggatgattct attttttccc ctagatatag aaattcattt tttattatca taatattttc
     1921 atcatactgg cctgtctcta tatagacact acttagtatg gctccaaaca tatcctttac
     1981 atggacaggc acagatgata ttaggcttac atctaccatt ttggaaactg catttaagaa
     2041 gctggatact attatattga ccaactcctt tatggcagat atataaattt gttcagtaag
     2101 ttctatattt tcatctacca tcatttccac caggtgttgg gaggactcct cagaatttac
     2161 aaagaggaaa agtccttctg catcacctag cattttcatt agcactgcat ctactattgt
     2221 ttcttctgcc ataatttctt tatatacttc attataattt aaaatttcta tggttggtac
     2281 tgtcatgctt gtaggtttgt ttattagttg ggacaggctg gtggcagcat tcccaccacc
     2341 aatgttggcc aattccttta taaggtctat ttgtaaggga gtaaattcac ctttattcaa
     2401 tcaaaccacc ttcagttctg tcacatagat taccaacatc taagattaat acaatttcac
     2461 cattgcctag aatagtagcc cctaaatact ccttcatctt ggataagggt ccacttaact
     2521 ttttaattac tgtttcctgc tggcctatta gctcatctac taaaagacca taatattgat
     2581 tgcccaactg aattaggatt aggtggttgt ctgaactgct ggattctaaa tttaattttt
     2641 catttaaacg aactacaggc actgccctat ccatataaat atagatttcg ctgttgtggg
     2701 actctacaat ttcttccggc ctgattttaa ctaccctttc aataatgcct aaaggaattg
     2761 caaagacttc ctcccctata taaaccatca aggtttggat tatcgataag gtaagaggta
     2821 acctgatgat aaagctggta cccttgtcca cttcacttat gacttcaata tttccaccta
     2881 agctactgat cttttgcctt accacatcca tgcctacgcc tctacctgat acattggtta
     2941 catcctttac agtggaaaaa ccttgatcaa aaattagctg gataatctcc ctttggctca
     3001 tgccttccgt gtctattccc ttttcttctg cactttgttt gattttatct gggtctatac
     3061 ctttaccatc atcacttaca ataatgacta ccctgttgcc ctcctgatag gcatttagct
     3121 tgattaatcc ctggtctggc tttcctaggg cccgcctttc ttctacagat tcaataccat
     3181 gatcagctgc atttcttatc aggtggacta aaggttctcc caattcgctt actatggtcc
     3241 tatctaactc agtatcttct ccttcaataa caaattcaat ctccttattt aattcctgag
     3301 ataaatccct taccattctt ggaaacctat taagtaccac atttactggc tgcatcctaa
     3361 tttttaagac taaatcctgt agttcagatg ttatccttcc tacatactct aagggctcat
     3421 gaatttcagg aatatcatag ttgttactta aatcttccaa cctggttcta taaattacca
     3481 gctctgatac caggttcata aacctatcca gcctacctaa atcaacccga atagactggt
     3541 tcatctgatg aacctttcta tcccttcttt tatttgaagc tggtttttcc ttggtggctg
     3601 ccttttcatc taccttgctt gtggtatctt ccatttcttt tactaaaaca ctttctattt
     3661 ctgcaatgcc ttctatcatg tctttaattg cagccttatc cagcttgctt aagtatacta
     3721 acataaatat tttatcatag tttccttctt ccagctcctc tggattagga tttgttttta
     3781 ttacatctcc ctgctgttcc agtttattta ctaccaaata ggccctggct ccttttaggc
     3841 tacagtagtc ttctacctta actaaaatac tataggcctt gtaacctctt tcataggccc
     3901 ct
//
LOCUS       DATGSI010000463         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_362204, whole genome shotgun sequence.
ACCESSION   DATGSI010000463 DATGSI010000000
VERSION     DATGSI010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362204"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..>1136)
                     /locus_tag="VK031_07165"
     CDS             complement(23..>1136)
                     /locus_tag="VK031_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53736.1"
                     /translation="WVGSDYEITPDNPAYVDFYIDGVRKHGQEFVLPKGGRQVVWFKW
                     HTPKNPTTVTVSASPSRGYIIDNPSKSLAIKDLDEYEPPDPRAKDPETGKVVEKPEWF
                     EMVNPPSLANQISNEWSVWSAYWQENWVWQENWVWQEDWRWQENWVWQENWVWQEDWR
                     WISAWRWIITGIDENGNVEGYWADEGYWVDEGYWVDKGEWVDKGEWVDEGEWIYYQNH
                     YDASISNYNMTFKPDERVPTVKQWYGRWEMCSGYGYNIETNMRVSTNDRSAVAQQGNS
                     ITYLPEYMYLGYLRLSDRIKNLEFKLKKNRYSTFNNRVHFTPLWYPDEKYTTYTRIID
                     VWTPAGHLYVNLDDYINIKGSVLDDYKAVPSKVNRR"
BASE COUNT          309 a          273 c          144 g          410 t
ORIGIN      
        1 ggagctttta atttacttaa agttatcttc tatttacttt actaggtacc gctttataat
       61 catctaaaac agaaccctta atattaatat aatcatctaa atttacatat aaatggcctg
      121 ctggtgtcca tacatctatt attctagtgt aagttgtata tttctcatct ggataccata
      181 gtggtgtaaa atgaactcta ttattaaagg tcgaatatct attcttcttt aatttaaatt
      241 ctaaattttt aatcctatca gataatctta aataacctaa atacatatat tctggtagat
      301 aggttattga gttgccctgc tgtgctactg ctgatctatc attagtagag actctcatgt
      361 tagtttctat attataacca taaccactac acatttccca tctaccatac cattgcttta
      421 cagtaggtac cctttcatct ggtttaaatg tcatattgta gttgcttatt gaggcatcat
      481 aatggttttg ataatagatc cactctcctt catctaccca ttctccttta tctacccatt
      541 ctcctttatc tacccaataa ccttcatcta cccaataacc ttcatctgcc caatagcctt
      601 ccacatttcc attttcatca attcctgtaa ttatccatct ccaagctgaa atccatctcc
      661 agtcctcctg ccatacccaa ttttcctgcc atacccaatt ttcttgccat ctccaatcct
      721 cctgccatac ccaattttcc tgccataccc aattttcttg ccaataggct gaccaaactg
      781 accattcatt agatatttga tttgctaggc ttggaggatt aaccatttca aaccactctg
      841 gtttctccac taccttccca gtttcagggt ccttggccct tggatctggt ggttcatact
      901 catctaaatc cttaatagcc aagcttttgg agggattatc tattatatac cctctggagg
      961 gtgaagccga tacagttact gttgtagggt ttttaggggt atgccattta aaccatacca
     1021 cttgccttcc accttttggt aagacaaatt cctgcccatg ttttctaact ccatctatat
     1081 aaaaatccac ataggcagga ttatcaggag ttatttcata atctgatcct acccag
//
LOCUS       DATGSI010000464         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_313497, whole genome shotgun sequence.
ACCESSION   DATGSI010000464 DATGSI010000000
VERSION     DATGSI010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313497"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VK031_07170"
     CDS             complement(<1..598)
                     /locus_tag="VK031_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53737.1"
                     /translation="MGIGIREKDIELIEKTIERLNSIISVKFVLDQEKNIEEIHIVSD
                     GQRSAKQLSRDVQSILIATYDLNVDYKKISIAELPELALKKDKARLKIEKISYEKHGK
                     KAKVQVVLKKDRQYFETSMEGPNTKRSVQIMLAEATLSAVEKAYGYGQVFVVEDVNSF
                     QLAIDKVAIVIIMCLRDGEEKRFIGSSLIKDDSNDAIVR"
     gene            complement(932..1063)
                     /locus_tag="VK031_07175"
     CDS             complement(932..1063)
                     /locus_tag="VK031_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53738.1"
                     /translation="MLLFEANSVYEKYEINYFWLHEHRKDDMIVNYLIKMILDNVLK"
     gene            1111..2091
                     /locus_tag="VK031_07180"
     CDS             1111..2091
                     /locus_tag="VK031_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HLS53739.1"
                     /translation="MKIAIVTDRSRESFIPSEEARLEDQQKSETVSAIKEILSTKYDC
                     ISLIADDNLINTLRDEKVDLVFNLCNGIEGDSKLAQLPAILEYANIPYTGSSILGHSI
                     AINKIFSSTIFTHSKIATPKFASISGMEDLDNIDLQYPILVKPSDEGSSRGIHQDSLV
                     FNEEDLRKKVQEELNTYNPPIMLNEYIEGKEVSVGIIGNGSAPNVLAIQEVDFSQLPD
                     DLLKFYSFEIKSYYKDKTRYHIPARLNEEEKRIIEKAALKAFNSLHLRDYARVDIILS
                     NGVPYVLEINSLPGLQKGRSSLYRMAEATGLGYEGLIFKIVEVARKRYGL"
BASE COUNT          710 a          400 c          345 g          813 t
ORIGIN      
        1 cccttacaat ggcatcatta ctgtcgtcct ttatcaagga ggatcctata aagcgttttt
       61 cctctccatc tcttaaacac attataatta caattgctac cttgtctata gctaattgga
      121 aagaattaac atcttcaact acaaagacct gaccataacc ataagccttt tcaacagcgc
      181 ttaaggtggc ctctgccaac attatttgaa cacttctttt agtatttggt ccttccatgc
      241 tggtttcaaa gtattgcctg tcctttttta aaacaacctg aaccttagcc tttttcccat
      301 gcttttcata agaaattttt tctatcttaa gcctagcttt atcttttttt aaggccaatt
      361 ctggcagttc ggctatacta atctttttat aatccacatt tagatcatat gtggcaatca
      421 aaatggattg tacatccctt gaaagttgtt tggcacttct ttggccgtca gacactatat
      481 gtatttcttc aatgtttttt tcctgatcta aaacaaattt tactgatatt attgaattga
      541 gtctctctat agttttttct attaattcaa tatctttttc cctaatccct atacccaacc
      601 aattcacctc atatctcttt ataaaggatt tttagttaaa tcgtataaat gtttaaaaac
      661 ctgtcattgg attaatttta tcattattga ttgcatactt caacaatatt cgcacccaat
      721 tattcatata ttttttgttg aatccatgct tctttctgct ttatatattg agacttgcac
      781 agtttggtag gcttacatca aatttcttac ttgaccaaag gctataaata agatatgcta
      841 taatacaagg catcttatca ctaattttgc cttgcttgtt tcttaaccta atttcataag
      901 actttttttg caattctttt attttatcat tttattttag cacattgtct aaaatcattt
      961 ttattagata gtttactatc atatcatcct ttctgtgttc atgtaaccag aaataattaa
     1021 tttcatattt ttcataaaca ctattggctt cgaataggag cattttaatg cttaaatttc
     1081 attatttaat atacatgagg ggggatttta ttgaaaattg ctattgttac agaccgttca
     1141 agagaaagtt ttattccatc agaagaggcc cgtttagagg accagcagaa atctgaaact
     1201 gtgtcagcaa ttaaagaaat tctatctact aagtatgatt gcatatcttt aattgctgat
     1261 gacaacttga ttaatacctt aagggatgag aaggtagatc tggtctttaa cctatgtaat
     1321 ggtattgaag gtgacagcaa actggcccaa ttgccggcta ttttagaata tgctaatatt
     1381 ccctacacag gatcatccat attagggcat tctatagcca ttaataaaat attttcttct
     1441 actattttca cacattccaa aatagctaca cctaagtttg catccatatc tggtatggaa
     1501 gatttggata atattgattt acaataccct atattggtaa aacctagtga tgaaggctca
     1561 agtagaggaa ttcatcagga tagtttagtt tttaatgaag aagacctaag aaagaaggtt
     1621 caagaagagt taaatactta taacccacct ataatgttaa atgagtatat agagggtaag
     1681 gaagtctctg taggtataat aggtaacggc agtgcaccta atgtattggc tattcaggaa
     1741 gttgacttct cgcaattacc agatgaccta ttaaaattct atagttttga aattaaatca
     1801 tactacaagg acaaaacaag gtatcatata ccagcccgct taaatgaaga agagaaaagg
     1861 ataatagaga aggctgccct taaggccttt aattcactac atttaagaga ctatgctagg
     1921 gtagatatta tacttagcaa tggtgttccc tatgtactgg agattaattc cttgcctgga
     1981 cttcaaaagg gtagaagctc cttatatagg atggcagaag ctacaggcct aggatatgaa
     2041 gggttaatat ttaaaatagt cgaggtggct agaaaaaggt atggactcta aaaagacaag
     2101 cttatttgtc tttcacctta tagattaaag ggaaatatag taagatatac tatatttccc
     2161 tttattaatt tatccatcat ctataatcat ccataaattc ttttgcatct tctggatccc
     2221 atttcctatc tactaaaggt ggcccatccc ttaggtaact tgttttgt
//
LOCUS       DATGSI010000465         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_455781, whole genome shotgun sequence.
ACCESSION   DATGSI010000465 DATGSI010000000
VERSION     DATGSI010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455781"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..910)
                     /locus_tag="VK031_07185"
     CDS             complement(263..910)
                     /locus_tag="VK031_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HLS53740.1"
                     /translation="MKAVIFDMDGVIIDSEPVHFEVEGELLEELGGVFDEEEHSSFVG
                     TTDYAMWDTFKREFNLKPSVEEMVEMKKERFIENLHRIPLVENVTKIMSLLQDNGYLM
                     ALASSNNRKAVDAIIERFHLGKYLAYSISGEDVKNGKPDPEIFLTAAENLNVDPENCL
                     VIEDAANGVKAAKAAGMKCIGFKNPNSGNQDLSKADLVMDSYDQFDLEKIQKLFK"
     gene            1081..1935
                     /locus_tag="VK031_07190"
     CDS             1081..1935
                     /locus_tag="VK031_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLS53741.1"
                     /translation="MKYHSKENIHPTDIVLRVQDIDRSEEFYREVLGLKLLERKDREI
                     SLTADGKKRLIRLISPENVVIKPPGRTGLYHFALLLPNRAQLALFFKHIRRLGYPLIG
                     ASDHGVSQAVYLEDLDGNGIEVYADIDINRWHRKEANTEIISQPLDYRGLIKEVEGLR
                     WKGLPQETIMGHIHLQVSDLEEAKKFYIDGIGLELIREIPNSAVFTASAGYHHHIAFN
                     IWNSKGAEPLPKESVGMEYYSLKFPNEGLLKKTVKKLSGLGFKSFENRGKIFVEDPSR
                     NKIALISQ"
     gene            complement(1960..>2293)
                     /locus_tag="VK031_07195"
     CDS             complement(1960..>2293)
                     /locus_tag="VK031_07195"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HLS53742.1"
                     /translation="SGEYIGQIDLLNIDWPNRVARIGIVIGVKKYLGRGYGTEAIKLL
                     KDFAFNRLNLHKLELEVRAFNKRAINCYKKCGFIEEGRLRENFYHKGRYEDTLLMGIL
                     KRDWENLQ"
BASE COUNT          730 a          432 c          388 g          743 t
ORIGIN      
        1 cctctgcagg cctggccact acaagattag gtttgattcc tattaaggcc tcctttgaag
       61 gtaaatcttc ttgcatatta atagaatcta atatttcaaa ggccatcctt ctatcttggt
      121 tgccataaag tatatataag gaatttttat ccatacttac ccccctaatt agtttaaaat
      181 taattattaa tatataggaa ttaaatcttt tagtcaaaat atgtataaaa aaggcagttt
      241 tacaactgcc aattacctct ctttatttga aaagcttttg tattttttcc aaatcaaatt
      301 ggtcataact atccataact aaatcagctt tagataggtc ctggtttcct gaattaggat
      361 ttttaaaacc tatacatttc atgcctgcag cctttgcagc cttaactcca ttggcagcat
      421 cttcaataac caggcaattt tctggatcta catttaagtt ttcagcagca gttaagaaga
      481 tttctggatc aggcttacca tttttcacat cttcgccact aatggagtat gccaaatact
      541 tcccaagatg gaatctctcg attatggcgt ctacagcctt tctattattg gatgaagcca
      601 aagccattaa ataaccatta tcctgtaaaa gagacatgat ctttgttaca ttttctacta
      661 ggggtattcg atgaagattt tcgataaatc tttccttttt catctctacc atttcttcta
      721 ctgatggctt taggttgaac tcccttttaa aggtatccca catggcataa tcagtagtac
      781 ctacaaagga ggaatgttct tcttcgtcaa aaacaccgcc taattcttcc aacaactctc
      841 cctctacttc aaagtgtaca ggttcactgt caattattac tccatccata tcaaatatga
      901 ctgccttcaa tttaccactc cttttctgat ttatttccta gctattaata tacatgatac
      961 cctatcttaa gtcaaagaat atgttttatg ttccaaagtg ggaaatattt atattgtgcc
     1021 ttactaccaa gatattcttc tgcttatagg gtatataaat agaaaatagg aggtggttag
     1081 atgaaatatc acagcaagga aaatatccac cctactgata tagtactaag ggtccaagac
     1141 atagatagat cggaagagtt ttatagggaa gttttaggtc taaaactact agaaaggaag
     1201 gatagagaga ttagcttaac agctgatgga aagaaaaggt taattaggtt aataagtccg
     1261 gaaaatgtag tgattaaacc acctggacgg actggcttat accattttgc cctgttatta
     1321 cccaataggg cccagttggc cttatttttt aaacacataa ggaggctagg ttacccatta
     1381 attggggcct ctgaccatgg ggtaagccag gccgtatatt tggaggacct ggatggtaat
     1441 gggatagaag tttatgcaga tatagatatt aataggtggc atagaaagga agctaataca
     1501 gaaataatta gccagccctt agattatagg ggacttatta aggaagttga gggtcttaga
     1561 tggaagggcc ttccccaaga aactattatg ggacatatcc atctacaggt gtcagactta
     1621 gaagaggcaa aaaaatttta tatagatggt ataggcttag agttaattag ggagatccca
     1681 aattcggcag tctttactgc ctctgcaggc tatcatcatc acattgcttt taatatatgg
     1741 aacagcaaag gggcagaacc tttaccaaag gaaagtgtag gtatggagta ctatagcctt
     1801 aaatttccca atgaaggact tttaaagaaa acagttaaaa aattatcagg cttaggattt
     1861 aaatcctttg aaaatagagg aaaaatattt gttgaggatc ctagtagaaa taaaatagcc
     1921 cttataagcc aataaaccat agggtataat tcctatggtt tattgcagat tctcccaatc
     1981 tctttttaat atgcccataa gcagggtatc ctcgtatcta cccttatgat agaaattttc
     2041 tctaagtcta ccttcctcta taaaaccaca ttttttataa cagttaatgg ctcttttgtt
     2101 aaaggcccga acttcaagtt ctaatttgtg caaatttaat ctgttaaagg caaaatcctt
     2161 aagtaacttt atagcctctg taccataacc tcggcctaag tattttttca caccaattac
     2221 tattccaatc cttgccaccc tattgggcca atctatattt agcaaatcta tttggcctat
     2281 atactctcca cta
//
LOCUS       DATGSI010000466         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_553252, whole genome shotgun sequence.
ACCESSION   DATGSI010000466 DATGSI010000000
VERSION     DATGSI010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553252"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VK031_07200"
     CDS             <1..841
                     /locus_tag="VK031_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HLS53743.1"
                     /translation="IMKYRIVADSSCDLNEGMQEQLNIDLVPFNISVDDEHFVDDKNL
                     DVPQLIKSMKKSPNPIKTSCPSPGDFLKYFRKADNIFGVTISANLSGTYNSAVLARDM
                     ALKEDPNKFVHIFNSKSASVGETLVALKIDELIKEKLNHMEIVEKVEEYIEQMKTYFI
                     LESLDNLVKNGRISKTKGLIASVLNFKPIMGEDGEGNIELVENIRGSKRAFNRLVEII
                     GETKENFEDKVLAISHANALEKAMALKEKIQELYNFKEILIIETKGLSTAYANDGGVI
                     LAF"
     gene            929..1012
                     /locus_tag="VK031_07205"
     tRNA            929..1012
                     /locus_tag="VK031_07205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:963..965,aa:Leu,seq:tag)
     gene            1214..2503
                     /gene="tig"
                     /locus_tag="VK031_07210"
     CDS             1214..2503
                     /gene="tig"
                     /locus_tag="VK031_07210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HLS53744.1"
                     /translation="MKAVFEGKEGNKVSFNFEIGAEEFEKAVQQAYLKNRSRFNIPGF
                     RKGRAPRKIIELNYGEGIFYEEALNSILPEQYEKAVEELELEPVDTPEVDIEEIEKGN
                     PILVKIEVDVKPEVKLGDYKSIELEKIEYNVTDEMIETEIESLQNANARLIDANDREA
                     KERDLLTIDFEGYVDGEKFPGGSAEGHQLELGSNTFIPGFEEQLVGKAKDEEVEVNVT
                     FPEEYHEESLAGKDATFKVKIHDIKEKQLPELDDEFAKDVSEFDTLEELREDIKERLE
                     KDLKEQERIEKENKVIEKVMEISEVDIPEGMIESQIDDELRQFDFRMRGQGLDLEQYL
                     KLTGMTVEDLREQFRPAATNRVNADLVLEEIAKVEEITATEEDIDEELKKMAEQYNAE
                     DIDEFIADMKKGQLEILKSGIINSKVIDLLLENVKFN"
     gene            2518..>2896
                     /locus_tag="VK031_07215"
     CDS             2518..>2896
                     /locus_tag="VK031_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HLS53745.1"
                     /translation="MALVPIVVEQTSRGERSYDIYSRLLQERIIFLGSEISDPIADLV
                     VAQLLFLEAEDPDKDIQIYINSPGGSVSAGFAIYDTMQYIKPDVSTICIGRAASMGAF
                     LLAAGTKGKRYSLPNSDIMIHQPL"
BASE COUNT         1138 a          364 c          614 g          780 t
ORIGIN      
        1 tattatgaaa tatagaatag ttgcagacag tagttgtgac ttaaatgaag gcatgcagga
       61 acaattgaat atagatttgg taccttttaa tatttctgtt gacgatgaac attttgtgga
      121 tgataaaaat ttagatgtgc cccagttaat taagtctatg aaaaaaagtc ccaatcctat
      181 taaaacatca tgtccgtctc caggagactt cttaaaatac tttagaaagg cagacaatat
      241 ttttggagta accatatcag ctaatttatc aggaacctat aatagtgcag ttttggctag
      301 ggacatggcc ttaaaggagg atccaaataa attcgttcat atatttaatt ctaagtctgc
      361 cagtgtaggc gaaactttag tagccttaaa aatagatgaa ttaataaagg aaaagcttaa
      421 ccatatggaa atagtagaaa aggtagagga atatatagaa cagatgaaaa cctactttat
      481 attagagagc ctggataatc tagttaaaaa tggcagaata tctaaaacaa aagggttaat
      541 tgcttcagtt ttaaatttca aacctattat gggggaagat ggagaaggaa acatagaatt
      601 ggtagaaaat atacgtggta gtaaaagggc ctttaatcgc ctggtagaaa taatcgggga
      661 aacgaaggaa aactttgaag acaaggtact agctatttcc catgccaacg cattggaaaa
      721 ggcaatggct ttaaaggaaa agattcaaga attatataat ttcaaagaaa tattaataat
      781 agaaaccaag ggcctaagta ctgcttatgc taacgatggt ggagttatct tagcatttta
      841 aaagtttgag gaaaatatcc tttaattttc aaaaaaataa ttgacaaaat actacagggt
      901 gatataataa ttaagcagtg aattttatgc gggagtggcg gaactggcag acgcgctaga
      961 cttaggatct agtgtctttg acgtgggggt tcaagtcctc tctcccgcac cacaaaaacc
     1021 aatggtttaa ccattggttt tttcctatgt ttatataagt ttctacatat tgggtatagg
     1081 ttgattagat aaaccgtaac catgaataag ccatattttt gtaatatata gattgttatg
     1141 ttattatatt gtgatataat gtttatggaa aacagtaaca aagatagaac aaatttgagg
     1201 aggatatagg ataatgaagg cagttttcga aggtaaagaa ggaaataaag tttcttttaa
     1261 ttttgaaata ggagcggaag aatttgaaaa ggctgtacaa caagcatatt taaagaacag
     1321 atcaagattc aacatacctg gtttcagaaa gggaagggct ccaagaaaga taatagaatt
     1381 aaactatggg gaaggtatat tctacgagga agctttaaat tcaatacttc ctgagcaata
     1441 tgaaaaggct gtagaagaat tagaattaga accagtagac acaccagaag tagatataga
     1501 agagattgaa aaaggaaatc cgattttagt taagatagaa gtagacgtaa agccagaagt
     1561 taaactaggt gactataaaa gtatagaatt agaaaagata gagtataatg taacagatga
     1621 aatgattgag actgagatag agtctttaca gaatgctaat gcaagattaa tagatgccaa
     1681 tgatagggag gcaaaagaga gagacctttt aactatagat tttgaaggtt atgtagatgg
     1741 ggaaaaattc ccaggtggct cagcagaagg acaccaatta gaattaggat ctaatacctt
     1801 tatcccaggc tttgaagagc aacttgtagg aaaagccaag gatgaagagg tagaagtaaa
     1861 tgtaactttt cctgaagaat accatgaaga gtctttagca ggtaaagatg ccacctttaa
     1921 agtaaagatc catgacatta aagagaagca actaccggaa ttagatgatg aatttgctaa
     1981 agatgtaagt gaatttgaca ccctagaaga actaagggaa gacataaaag aaagattaga
     2041 aaaagactta aaagagcaag aaagaataga aaaagaaaac aaggtaattg aaaaggtaat
     2101 ggaaatttca gaggtagaca taccagaagg tatgatagaa tctcaaatag atgatgaact
     2161 aaggcaattt gatttcagaa tgagaggtca aggactagat ttagagcaat atctcaaatt
     2221 aacaggaatg acagtagaag atttaagaga gcagtttaga ccagcagcaa ccaatagggt
     2281 aaatgctgat ctggttttag aggaaattgc taaagtagaa gaaattacag caacagaaga
     2341 agatatagat gaagaactta agaagatggc tgaacaatac aatgcagagg atatagatga
     2401 attcatagcc gatatgaaga agggccagtt agaaatttta aaaagtggta taattaatag
     2461 taaagtaata gacttattac ttgaaaatgt gaaatttaat taattggagg gattcagatg
     2521 gcattagtac caatagtagt agaacaaacc agtaggggtg aaagatccta tgatatttat
     2581 tccagattat tgcaggaaag aataatattc ttaggttcag aaataagcga tcctattgca
     2641 gatttagtag tagcccaatt attgttccta gaagctgaag acccagataa agatatccaa
     2701 atatatataa acagtcctgg tggctcagta agtgctggat ttgctattta tgatactatg
     2761 caatatatta aaccagatgt atccaccatt tgtataggaa gagcagcaag catgggtgca
     2821 tttttattag ctgccggaac taagggtaaa agatactcct tacccaattc agacataatg
     2881 atccaccaac ctttag
//
LOCUS       DATGSI010000467         3187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_694227, whole genome shotgun sequence.
ACCESSION   DATGSI010000467 DATGSI010000000
VERSION     DATGSI010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694227"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VK031_07220"
     CDS             complement(<1..455)
                     /locus_tag="VK031_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53746.1"
                     /translation="MKTFNKKEAEGILKSHPVIKELKESIISMSKSPHAHLVGGAVVD
                     ILEGREPKDYDFNVSFCGKGWVKINENPDFKFLYNSATAITYLFKGKYVVQILEKQSE
                     YFPFFIEKFKYNIVTETMVTPNPFTEVSFNSKKLIPFGEVFKDGSITHKA"
     gene            complement(465..683)
                     /locus_tag="VK031_07225"
     CDS             complement(465..683)
                     /locus_tag="VK031_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53747.1"
                     /translation="MEVTAKYEDLMKERWLRQRDNGEIQWETKDGRRIPIKDISDEHL
                     ENTIRVVEKMRAKSKHYDDMGVWDDPNY"
     gene            complement(807..1361)
                     /locus_tag="VK031_07230"
     CDS             complement(807..1361)
                     /locus_tag="VK031_07230"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLS53748.1"
                     /translation="MKTIFLTAGHHISDQGASGSKFKENTLTLEFINLVTTYAKELNP
                     YINIWNDDFKDNLSTVISQVKSKGTREDLWIEVHFNAFNTKAIGYEAIVSDNASDRSK
                     QVANDLSFIPDIVGIANRGVKTESDSARGKLGMFRTVVPSVLLELGFIDNDSDMAKWK
                     DKRVEVAKELAKYLNNLYTDGWKE"
     gene            complement(1367..>3187)
                     /locus_tag="VK031_07235"
     CDS             complement(1367..>3187)
                     /locus_tag="VK031_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53749.1"
                     /translation="DDGGSVIETHLGDKLEHDFNRYVNVLYFGLGKPGGAEVDAEFDN
                     LLDFLNKDTSETVVYFPKGYKYRISSTKTFRTQVNIEAEASINYTGDHTEPAIVVGSP
                     VQRNNGRSIRLGVTSTENRSTWDSGDGDKGIVLYNCYSSTIRVGASFFKTGVELIGTN
                     GNGFVYNEITLSNFNGNHEALVLNSDNEGWCNENNFFGGRIHASGGTPSNDNRYGVVL
                     RSGDGYRQNNNIFYKTSIELNSGGVDSPYRAIAIKIENGRQNEWVALRDETNSLTVQT
                     FNDSHNNKIHATFSVVGASYLSRVEDLGDYPSTIFTSNQQLINNTRGWELLNITNVND
                     YFFESTDDGKVSVLSPLKIQSDSSSNPSSSPTDSLQLNSDTNSIELTTLAGFGVRINT
                     QIRKRFTTSITTVDKDNLGRIYIVCRDENQEIIPDSFDLVRISGRHSKVISSRFGGGV
                     ALVFSYNIDSGNFMHFFCVDDVVKYVDIFYSGYGSTPVSLKSVNLYSIDGSTDLIPYN
                     TNIIKTADSMPNNEVGSVGDFRIKRTGDEIGYIKKTSGWVEVGKEATTTTKGEVLMAE
                     ASDNTADPVGSEYNQNEVQGILDELRDLKSKMRSAGLLES"
BASE COUNT          899 a          653 c          456 g         1179 t
ORIGIN      
        1 gccttgtgag ttatagagcc gtccttaaac acttctccga acggtattaa tttcttgctg
       61 ttaaaactta cttctgtgaa agggtttggc gtgaccattg tttctgtaac tatgttgtat
      121 ttaaattttt cgatgaaaaa cgggaagtat tccgattgtt tttcaagtat ttgaactacg
      181 tacttccctt tgaaaaggta ggtaatagct gtagctgaat tgtatagaaa tttgaaatct
      241 gggttttcgt taatcttaac ccaccctttt ccacaaaaag aaacgttaaa atcatagtct
      301 ttaggctcac gcccttccaa tatatccacc accgcccccc ctactaagtg tgcgtgaggt
      361 gattttgaca tagatattat agactcttta agctccttta ttacagggtg ggattttaga
      421 ataccctccg cttctttttt attaaatgtt ttcatagtta ttgtttaata gttagggtcg
      481 tcccatacgc ccatatcgtc ataatgttta gatttagccc tcatcttctc caccactctt
      541 attgtatttt ctaagtgttc gtcactaata tcttttatag gtatccttct tccgtcttta
      601 gtttcccatt gtatctctcc gttatccctt tgacgtagcc acctttcttt cattaaatct
      661 tcgtattttg ctgttacttc catatcttta tcttttaatg ttcaactgca atattacaga
      721 atagttttca tttatccaaa tatttacaaa taaaaaaccc gacaactgct tagctatcgg
      781 gcttaattaa aggtttatgt tatacgttac tctttccaac catctgtgta taagttgttt
      841 aggtacttag ctaactcctt ggctacttcc actcttttat ctttccattt agccatgtca
      901 ctatcattat ctatgaaccc taattctaaa agaactgaag gaaccactgt acggaacatt
      961 ccaagcttgc cccgtgctga atcactctct gtcttaaccc ctctgttagc tatcccaaca
     1021 atgtctggaa taaaggagag gtcgttagct acctgtttgc ttctgtcgct tgcattgtca
     1081 gatactattg cttcataacc tatagccttt gtgttgaaag cgttgaagtg aacttctatc
     1141 cataaatctt ctcgtgtacc tttagacttt acctgtgata ttacggtgct taggttatct
     1201 ttaaagtcat cgttccatat atttatgtat ggattaagtt ctttggcata agtggtgact
     1261 aagttaataa actcaagagt aagtgtattc tctttgaact tacttcccga agccccttgg
     1321 tctgaaatat gatgtcctgc tgtaagaaat attgttttca tattaattag ctttctaaca
     1381 gtcctgccga cctcatttta gattttaaat ctcttagttc atctaatatc ccctgtacct
     1441 cgttttgatt gtattctgat cccactggat cagctgtgtt gtcagaagct tctgccatta
     1501 gaacctctcc cttagtagta gtagtagctt ccttacctac ttctacccac cctgatgttt
     1561 ttttaatata ccctatctca tcaccagtcc tctttattct aaagtcacca acacttccta
     1621 cttcattgtt tggcatagaa tctgctgttt ttataatatt tgtattataa ggtattaaat
     1681 cagtgcttcc atcaatagaa tataaattaa ctgatttaag actgacagga gtagatccat
     1741 atccactata aaatatatct acatatttaa ctacatcatc aacacaaaag aaatgcataa
     1801 aatttccaga atcaatatta taggaaaata ctaatgcaac tcctccccca aacctagatg
     1861 aaataacttt ggaatgtctt ccacttattc ttaccaagtc aaaagaatct gggattatct
     1921 cctgattttc atctctacat acaatataaa tacgtcctaa attatcctta tctacagtag
     1981 ttatagaggt agtaaatctt tttcttatct gggtgtttat tcttacccca aaacccgcta
     2041 acgttgttaa ttctatagag ttagtatcag agttaagctg taaactatct gtaggagaag
     2101 aagaagggtt tgaagatgag tcactttgaa tttttaatgg ggatagtact gatactttgc
     2161 catcatctgt tgattcaaag aaataatcat ttacattagt tatattaagt aattcccatc
     2221 ctctagtatt atttattaat tgttgattac ttgtaaatat agtactagga taatccccaa
     2281 ggtcttcaac cctgcttaaa taacttgctc ctactacaga aaaagtagca tgtattttgt
     2341 tattatggga atcattaaaa gtctgtactg tcagagaatt agtctcatcc ctcaatgcta
     2401 cccactcgtt ttgtcttcca ttttctattt taatagctat tgctctataa ggagaatcta
     2461 ctcctcctga gtttagttct attgaggttt tgtagaaaat attattgttt tgtctgtatc
     2521 cgtcacctga tcttaaaaca acaccatatc tattatcatt agagggagtg cctcctgaag
     2581 catgtattct tcctccaaag aagttatttt cgttacacca tccttcatta tcactattta
     2641 aaactagggc ttcatggtta ccattaaaat ttgatagagt tatctcattg tacacaaacc
     2701 cattaccatt agttcctatt aattccacac ctgttttaaa aaatgatgct cctactctaa
     2761 ttgtagaact atagcaatta taaagtacta tacctttatc cccatctcct gaatcccatg
     2821 tagatctatt ttcagttgag gtaactccta gtctaatgga tctaccattg tttctttgca
     2881 caggagatcc tactactatt gcaggttctg tatgatcccc tgtataatta atacttgctt
     2941 ctgcttctat atttacttga gttctaaaag tcttagttga ggatattcta tatttataac
     3001 ccttagggaa gtatactact gtttctgagg tgtctttatt taggaagtct aagagattat
     3061 caaattctgc atctacctca gctccacctg gtttacccag tccaaaataa agaacattca
     3121 catatctatt aaaatcatgt tctaatttat cccctaaatg agtttctata acacttcctc
     3181 catcatc
//
LOCUS       DATGSI010000468         1383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_647745, whole genome shotgun sequence.
ACCESSION   DATGSI010000468 DATGSI010000000
VERSION     DATGSI010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647745"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1383
                     /locus_tag="VK031_07240"
     CDS             <1..>1383
                     /locus_tag="VK031_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HLS53750.1"
                     /translation="MDYLKGLNDRQKEAVLETEGPILIMAGAGSGKTKVVTHKIAYLI
                     EEKKIFPGNILAITFTNKAADEMKERVAKLLKKDVEGMWIGTFHAVCARILRRDIDKI
                     GYDRSFSIYDRDDQITLVRECINELNINKDLFKESSILARISSLKDEQISPDEYINEN
                     YNNYHHRKTGEIYDLYVKKLKEYNALDFDDLILKAVELLNNHSEILSYYQKIFQYVFV
                     DEYQDTNKIQYELVKLLSGGHENICVVGDSDQSIYSFRGADISNILDFEKDFPGAKVI
                     LLEQNYRSTQTILNAANQVIKYNFGRKEKNLWTANSQGKEIIYEELENSDEEAQYVAQ
                     KIHHLIYKGHKPSDIAILYRTNAQSRAFEEAFMKEELPYKIVGGLRFYDRKEVKDVLA
                     YLRVLQNPTDRISLKRIINTPRRGIGAATVAKIEEYADSNGLSMYQALLSLNQIDGLS
                     TRAKNRVQDF"
BASE COUNT          519 a          197 c          290 g          377 t
ORIGIN      
        1 aaatggatta tttaaaagga ttaaatgata gacaaaaaga ggctgtgctg gaaacagaag
       61 ggcctatact tataatggca ggagcaggca gtggaaaaac aaaagttgtt acccataaaa
      121 tagcctactt aattgaggaa aaaaagattt tcccaggaaa tattttggca attaccttta
      181 ccaacaaggc tgctgatgaa atgaaggaga gagtggccaa gctactaaaa aaagatgtgg
      241 agggaatgtg gataggaacc tttcatgctg tctgtgccag gattttaagg agagatattg
      301 ataaaatagg ttacgacaga tcctttagta tatatgatag ggatgatcag attaccctag
      361 ttagagaatg tataaatgag cttaatataa ataaggattt atttaaggaa tcctcaatcc
      421 tagccaggat aagctcttta aaagatgaac aaattagtcc agatgaatat ataaatgaaa
      481 attataataa ttaccaccat agaaaaacag gtgaaatcta tgatttatac gtaaaaaaat
      541 taaaagaata caatgccctt gactttgatg atttaatact taaagcagtg gaattattaa
      601 ataatcatag cgagatttta tcttattatc agaaaatctt tcaatatgtt tttgtggatg
      661 agtatcagga tacaaataaa atccagtatg agttggtaaa gctcttatct ggcgggcatg
      721 aaaacatctg tgtagtagga gactcagatc agagtattta ttcctttaga ggggcagata
      781 tatcaaacat tttagacttt gagaaagatt ttcctggagc caaagtaata cttttagagc
      841 agaattaccg ctccactcaa accatcttaa atgctgctaa tcaggtgata aagtataatt
      901 ttggtagaaa agagaaaaac ttatggacag ccaattctca aggtaaagag ataatttatg
      961 aagaactgga gaactcagat gaggaggccc aatatgtggc ccaaaagatc caccatttaa
     1021 tttacaaggg gcataaacct agtgatatag caattcttta taggaccaat gcccagtccc
     1081 gtgcctttga agaggccttt atgaaggagg agttaccata taaaatagtt ggaggtttaa
     1141 ggttctatga tagaaaggaa gttaaggatg tgctggcata tttaagggtc ttacagaatc
     1201 cgacagacag aatatcctta aaaagaatta taaatactcc aagaagggga attggagctg
     1261 caacggtagc taaaatagaa gaatatgcag acagtaatgg tttgtccatg tatcaggccc
     1321 tattatcctt aaaccaaata gatggcttgt ctaccagggc aaaaaacagg gtccaggact
     1381 ttg
//
LOCUS       DATGSI010000469         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_313953, whole genome shotgun sequence.
ACCESSION   DATGSI010000469 DATGSI010000000
VERSION     DATGSI010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313953"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="VK031_07245"
     CDS             <1..1558
                     /locus_tag="VK031_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HLS53751.1"
                     /translation="GMTIMGSGLQKAAGARLKKVIEVLTTNIFMGILVGALVTMVIQS
                     SGATTVMVIGFVNAGIMTLLQATGVIMGANIGTTVTAQLIAFNLTDYAPIAVIVGVGL
                     WITTSKKRTKDLAEILIGVGILFIGMGMMGDGLSPLSESPAFTRLMISLDNPVLGMLT
                     GFALTTLVQSSSASIGLLQALGGQGLISINLAFPILFGENIGSTTTALLSSVSANKTA
                     KRAAIIHFLFNVIGTIIFMTVLRYPIMWIVTRLSPDSIPRQIANAHTLFNLVNVIIQL
                     PFASFLVRIAEWVVPGEDEKEKMASLYLDERIIETPSIALGQVNKEVLRMGDIVLENL
                     KKSKEVLLNNKIDYIPEVLEGEKLINKIEKEITEYLVKISNAPLSDEQQNEVNTFIYT
                     INDLERIGDHIDNIIELSQHMSEQAIEFSTDAVEGLEKMFDKCVKIVKKTREAFEFNN
                     MDLVREVELIEEKVDKLEKENRKGHIDRLNKMECQTEPGIIFLDALSNLERVADHSLN
                     IAMYVYDRLK"
     gene            1928..>2052
                     /locus_tag="VK031_07250"
     CDS             1928..>2052
                     /locus_tag="VK031_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLS53752.1"
                     /translation="MYDVVIIGGGPAGLSAGLYAARARLKTLIIEKEAYGGQIATT"
BASE COUNT          737 a          270 c          416 g          629 t
ORIGIN      
        1 tggtatgaca attatgggtt ccggcttaca aaaggctgca ggtgctaggt tgaaaaaggt
       61 aatagaggtt ttaaccacta atatttttat gggcattttg gtaggagcct tggtaaccat
      121 ggttatccaa agtagtggag caactactgt tatggttata gggtttgtaa atgctggtat
      181 tatgaccttg ttacaggcaa caggggtaat aatgggtgcc aatattggta ctactgtaac
      241 agcacaatta atagccttta acttaacaga ctatgctcca atagctgtta tagtaggggt
      301 aggcttatgg attaccactt ccaaaaaaag aactaaagat ttggcagaaa ttttaatagg
      361 tgtaggaatt cttttcatag gcatgggaat gatgggagat ggcttaagcc cattatcgga
      421 atcaccagcc tttactagat tgatgattag cctagataat ccagttctag gtatgttgac
      481 aggatttgct cttaccactc tggttcaaag tagtagtgcc tctattggtc tattacaggc
      541 cttaggtgga cagggactta ttagcataaa tttagctttt ccaatacttt tcggggagaa
      601 tatagggtct acaactacag ccctactatc cagtgtaagt gccaataaaa cagcaaaaag
      661 agctgcaatt attcactttt tatttaatgt tataggaaca attattttta tgacagttct
      721 aaggtatcct attatgtgga ttgtaaccag attgtcacct gatagtattc caaggcagat
      781 agccaatgca cataccttat ttaatctagt gaatgtaata atccaacttc cctttgccag
      841 cttcttagta agaattgcag agtgggtagt accaggagaa gatgaaaaag aaaagatggc
      901 ttctctttac ttagatgaaa gaattattga gacaccttcc atagccttag gccaggtaaa
      961 caaagaagtt ctaagaatgg gagatatagt tttagaaaat ttgaaaaagt caaaagaagt
     1021 attgctaaat aataaaatag actatattcc cgaagtttta gaaggggaaa aactaatcaa
     1081 taaaatagaa aaagaaataa cagaatactt ggtaaagatt tccaatgcac ccttatcaga
     1141 tgaacaacaa aatgaagtaa atacctttat ttatactatt aatgatctgg agagaatagg
     1201 ggatcatata gataatatta tagaactatc ccaacatatg tctgaacagg ccattgaatt
     1261 cagtacagat gcggtggaag gtctggagaa aatgtttgat aaatgtgtaa aaatagttaa
     1321 aaagactagg gaagcttttg aatttaataa tatggacttg gttagagaag tggagcttat
     1381 agaggaaaaa gtagataaac tggaaaaaga aaatagaaaa ggccatatag acaggttaaa
     1441 taagatggaa tgtcaaactg agcctggtat catattttta gatgccctta gtaatctgga
     1501 aagagtggca gatcactcct taaatatagc catgtatgta tatgataggc tgaagtaatc
     1561 taatagattc aggattttta aattataaaa ctttttctat tgtaataagc tttataatag
     1621 attaagatga aattagattg tcaaataagt catatattta aatatttgat acatacacag
     1681 gctagtttat tctcaaatag ctttaccaaa ttatagaaac tagtttaaaa gggagtaact
     1741 atagtcttta agggatatta gataacaaat agagtaaatg ggctgaagtc tatttactct
     1801 atttatttat aaaggagtct ttaatttctt taaaagatac agttatttat ttaattagta
     1861 tatataatat agggtaaaaa taaaaaggat ttaaatttgt attaaatact aaggaggata
     1921 aaagatgata tatgatgttg taattatagg tggaggaccg gcaggcctat ctgcaggttt
     1981 atatgcggct agagcccggt taaaaacatt aattattgaa aaggaggcct atggtgggca
     2041 gattgccacc ac
//
LOCUS       DATGSI010000470         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_222753, whole genome shotgun sequence.
ACCESSION   DATGSI010000470 DATGSI010000000
VERSION     DATGSI010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222753"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VK031_07255"
     CDS             <1..1103
                     /locus_tag="VK031_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53753.1"
                     /translation="IKSVLRRVDVDDNNCRVIYSDGNDTKLKVSRGKTTDEPKKINFI
                     TSKAFEGADIYDVDGKVVVISDGNREHTLLDISTKLIQITGRIRDTKYFDEIYHITAF
                     NRYDDNKLTYDEYRTSILTSLSKDEEFINYNIKRFKEQVVKYVENGVAVFHYLDKEGN
                     VKYDSNRIKIDLYNYKIANEIYKNRINLTSAYNRNNLNNSEFKTVDNSHIEIKNLDDV
                     VNDFKVAIEAIKERYEKGEEYLSLVMGYYEQFPYLLEAVSKLGFKEIKEMKYRTSNIK
                     RKLEMMKLTNKEDSYIDKVKVALRYNTGIKVGMGYTGKELKELLQKTYDEVGCQSKAK
                     GVDINDYYLTTKKSVREDNKVVTKTFIVAKKH"
     gene            1173..1889
                     /locus_tag="VK031_07260"
     CDS             1173..1889
                     /locus_tag="VK031_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53754.1"
                     /translation="MKNSIKNTILVAVMVVLGVFTIGCSKDEIVPEYNFEEPEEEKYL
                     VTAFRLKVYYTKLNLVDTTYRENGGKIVETKVVPKGDISITNFREGKYDLVDSTDFWY
                     YTSVFEFNHNVNGGKWVSKNGTLFRGLYKEGYVHKVVVEMSDIDSYDTNKGYDPKRLD
                     MITKRTFIEAFKETTYNRFKANYIKYLGRGIERGYIFYIPDFRDEVSFGNEYIEEEEY
                     LEFKDVPYYDYFSSVRKKVK"
     gene            2054..2209
                     /locus_tag="VK031_07265"
     CDS             2054..2209
                     /locus_tag="VK031_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53755.1"
                     /translation="MTNKANKNNDRLKTIQIICATVVTLAGGVGVMLSMPYAIWLLIA
                     GVIFFLN"
     gene            2283..>2695
                     /locus_tag="VK031_07270"
     CDS             2283..>2695
                     /locus_tag="VK031_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53756.1"
                     /translation="MKQIYDKPIPTDPNYQIVYNEKRIEKMEEEIAMIKSKRATKSTP
                     FILKELEGRIKALKAYPKAQFEFTKDQYAPFDAYVKSEDIPEVIKSIIEIKDRSVAST
                     KYYDAQFELGKLTKMINIAQSRGINNVIFMAHYTD"
BASE COUNT         1116 a          342 c          479 g          758 t
ORIGIN      
        1 tcattaaaag tgtccttcgt agagttgacg tagatgataa caactgtaga gttatatact
       61 ctgatggtaa tgatacgaag cttaaagtta gtagaggtaa gactactgat gagcctaaga
      121 agattaactt tattacttct aaagcttttg aaggagcaga tatttatgat gtagatggaa
      181 aggtagtagt aatcagtgat ggtaacagag aacatacctt attagatatt tctactaagc
      241 ttatacagat tactgggaga attagagata ctaaatactt cgatgaaatc tatcacataa
      301 cagcttttaa tagatacgat gataataagc ttacctacga tgaatacaga acttctatac
      361 ttacttctct tagtaaggac gaagagttca ttaattacaa tataaagagg tttaaagagc
      421 aggtagttaa gtatgtagag aatggagtag cagtattcca ctacttagac aaagaaggta
      481 atgttaaata cgattctaat aggattaaga tagacctata taactataag attgctaatg
      541 agatatataa gaatagaata aaccttactt ctgcttataa cagaaacaac ctaaacaact
      601 ccgagtttaa gacagtagat aactctcata tagagataaa gaacttagat gacgttgtaa
      661 atgattttaa ggtcgctata gaagctataa aagaaagata cgagaaagga gaagagtatc
      721 tatcattagt aatgggttat tacgagcaat tcccttactt attagaagca gtatctaaat
      781 tagggttcaa agaaattaaa gaaatgaagt atagaacatc aaacattaag aggaaattag
      841 aaatgatgaa acttactaac aaagaagatt cttatataga taaagttaag gttgctttga
      901 gatataatac aggaattaaa gtaggtatgg gatatactgg taaggaactt aaagagcttc
      961 tccagaaaac ctatgatgag gtaggttgcc aatctaaggc taaaggggtt gatataaacg
     1021 attattacct aactactaag aagagtgtta gagaagataa taaagtagta actaagacct
     1081 ttatagtagc taagaagcat taaacctttt aatattaaaa cagtctaata agtaacagaa
     1141 taaaataata acaaattaaa acaaataaca aaatgaaaaa ttcaatcaaa aacacaattt
     1201 tagtagcagt gatggtagta ttaggagtat tcactatagg atgtagtaaa gatgaaatag
     1261 ttcctgaata taattttgaa gaacctgaag aggagaagta tctcgtaact gcatttagat
     1321 taaaagtcta ttatactaag ttaaatctag tagatacaac ttatagagag aacggaggaa
     1381 agatagtaga aactaaagta gttccaaagg gggatatatc tataacaaat ttcagagaag
     1441 gtaaatatga tttagttgac tctactgatt tttggtatta tacttctgtt tttgaattta
     1501 accataatgt taatggtgga aagtgggtat ctaaaaacgg tactttattc agaggacttt
     1561 ataaagaggg ttacgtacat aaggtagttg tagaaatgtc tgacatagac agttatgata
     1621 ctaataaagg ttatgaccct aaaagattag atatgattac taaacgtaca tttatagaag
     1681 cgtttaaaga aactacctac aatagattta aagctaatta cataaaatac ttaggtagag
     1741 gtattgagag aggctatata ttttatatac ctgattttag agatgaagta agtttcggga
     1801 atgagtatat agaagaagaa gaatacctag aatttaaaga cgtaccttac tatgattatt
     1861 ttagttccgt ccgtaaaaaa gtaaaataag aagaatagta aaaccaatta taacctaata
     1921 ttatgtaata acctactaac caaaggttta tacaaaaaga ccctagtcaa atacattgaa
     1981 aacaattccg tatattagta gtacagattc acagagataa acaaatttaa caactaaaaa
     2041 acaaaatatc aaaatgacaa acaaagcaaa caaaaacaac gacagactaa aaacaatcca
     2101 aatcatttgt gcaacagtag ttactctagc aggaggagtc ggagtaatgt tatcaatgcc
     2161 ttatgctata tggctattaa tagcaggagt tattttcttt cttaattaat gtaaagggat
     2221 gtactccccc aatgtaaaac aaatatagat aaacaaacta acatcaacaa acctaaaaga
     2281 aaatgaaaca aatttatgac aaaccaattc caacagaccc taactaccag atagtttata
     2341 acgagaaaag gatagagaag atggaggaag agattgcaat gattaaaagt aagagagcta
     2401 ctaaatcaac accatttata cttaaagagt tagaaggtcg tattaaagcc ttaaaagctt
     2461 accctaaagc acagtttgaa tttactaaag accagtatgc accttttgat gcttacgtaa
     2521 aatcagagga cataccagaa gtaataaagt ccataataga gataaaggat aggagcgttg
     2581 cttctactaa gtattacgat gcccaatttg agttaggtaa gttaactaag atgattaata
     2641 tagcacagag tagaggtatt aataacgtaa tctttatggc tcactacaca gacaa
//
LOCUS       DATGSI010000471         1569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_222833, whole genome shotgun sequence.
ACCESSION   DATGSI010000471 DATGSI010000000
VERSION     DATGSI010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222833"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VK031_07275"
     CDS             complement(<1..182)
                     /locus_tag="VK031_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53757.1"
                     /translation="MSYTWIDKCVDGIIDHCYSRDIYEICNTLEIVIRKLDEDHELLQ
                     GNEALYIRDFLGDEII"
     gene            complement(245..517)
                     /locus_tag="VK031_07280"
     CDS             complement(245..517)
                     /locus_tag="VK031_07280"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLS53758.1"
                     /translation="MVSRNVYRIIQSRQAVKYIEKQDRIFKERIEKAYTEILNNPKDP
                     RKLLKGFKNKYRHRMGSYRIIYSVCDAKLVIEIILVDSRGQVYNRH"
     gene            complement(490..969)
                     /locus_tag="VK031_07285"
     CDS             complement(490..969)
                     /locus_tag="VK031_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53759.1"
                     /translation="MGKVTTTRTRLTMRNLNTYKSPAIHIHHLSDIVYNENKGNIYYT
                     PSASSYPEHWSTGRSEHFMIYKMNNSDIQFFFNRVDAFIKFAPINLKDEFLREMAEVL
                     KTMHQLSELSLLEQFVEEWMDTVYLYSNPDTIREIEEAEEELAKGEGVEWYPGMYIE"
     gene            complement(985..1311)
                     /locus_tag="VK031_07290"
     CDS             complement(985..1311)
                     /locus_tag="VK031_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53760.1"
                     /translation="MGKKTLKFIYEKSDDYREFTVDGMFGGVSPYGLLQAYLTEETIN
                     IPEDELYNIIDGGRLEKKDSTNIENIVKKSVHAKINIPRETIPSIVEWLNAKYDEYKE
                     IKKGNY"
     gene            complement(1335..>1569)
                     /locus_tag="VK031_07295"
     CDS             complement(1335..>1569)
                     /locus_tag="VK031_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53761.1"
                     /translation="YLLGRTSDRNLNSTVPNDDNLFPEEFTDPKEARAYVNKHQIFGS
                     GGFDADKLDDEEILEFANALLEQMKMVSYKYRK"
BASE COUNT          443 a          315 c          206 g          605 t
ORIGIN      
        1 aatataatct catcccctaa aaaatctctt atatataatg cttcatttcc ttgtaatagt
       61 tcatggtctt catctaattt ccttattaca atttctaatg tattgcatat ctcataaata
      121 tccctagagt agcagtgatc tattattcca tccacacact tgtcaatcca ggtataactc
      181 atgtcatcac ctcataaacc tatgttctta cgtgtttaaa ataacagaca ttaatctatt
      241 ttgactaatg tctgttatat acctgtccac gggaatcaac tagtataatt tctataacta
      301 gtttggcatc acatactgaa taaataattc gatagcttcc cattctatgt ctatacttat
      361 ttttaaaacc ttttaacagc ttccgaggat cctttgggtt attaagaatt tcggtataag
      421 ctttttcaat tctttcctta aatattctat cttgtttttc tatatacttt acggcctggc
      481 gagattgaat tattctatat acattcctgg ataccattct accccttctc ccttagcaag
      541 ctcctcctcc gcttcttcta tttcccttat tgtgtcagga ttactgtata gatatactgt
      601 gtccatccat tcttcaacga attgctcaag tagggataat tcagaaagtt ggtgcatagt
      661 tttgagtact tccgccattt ctcttaaaaa ttcatctttt agatttatag gtgcaaattt
      721 aataaaagca tctactctat taaagaagaa ttgaatatca ctgttattca tcttataaat
      781 catgaaatgc tcactccttc cggtggacca gtgctcagga tatgagctgg cacttggcgt
      841 atagtatata tttcccttat tttcattata tactatatct gataggtggt gaatatgaat
      901 agcaggactt ttatatgtat taagattgcg catagttaac cttgttctgg ttgtagttac
      961 cttacccata ccattagctc ctttctaata atttcccttt ttaatctctt tgtattcatc
     1021 atatttcgcg tttaaccatt ctactatgga aggaattgtt tctctaggaa tatttatctt
     1081 tgcatgaaca gattttttta ctatattttc aatatttgta ctatcttttt tctctagcct
     1141 tccaccatca ataatattgt ataattcatc ttcaggtata tttatagttt cctcagttag
     1201 ataagcctgt aataggccat aaggactgac accaccaaac attccatcta cagtgaactc
     1261 cctatagtca tcacttttct catagataaa ttttaaagtt tttttaccca tttaaatcac
     1321 tccttttaca aatattactt cctatactta tagctaacca ttttcatttg ctcaagtaga
     1381 gcattagcaa attccaatat ttcttcatca tctagcttgt cagcatcaaa ccctccagaa
     1441 ccaaatattt gatgtttatt tacgtatgca cgtgcttctt tagggtcggt gaactcttct
     1501 gggaagagat tgtcatcgtt ggggactgtt gaattaagat tcctatcgct tgtacgacct
     1561 aggagatag
//
LOCUS       DATGSI010000472         5069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_222864, whole genome shotgun sequence.
ACCESSION   DATGSI010000472 DATGSI010000000
VERSION     DATGSI010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222864"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /gene="eno"
                     /locus_tag="VK031_07300"
     CDS             complement(<1..507)
                     /gene="eno"
                     /locus_tag="VK031_07300"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HLS53762.1"
                     /translation="MSMIIDVYAREVLDSRANPTVEVEVVTESGAFGRALVPSGASTG
                     AFEAVELRDGDKSRYLGKGVLKAVKNVNEIIADEIIGMDVFDQLAIDQLLIDLDGTPN
                     KGKLGANAILGVSVAVARAAADALGMPLYQYIGGINAKQLPVPMMNILNGGEHADNNV
                     DIQEFMIMP"
     gene            complement(593..2125)
                     /gene="gpmI"
                     /locus_tag="VK031_07305"
     CDS             complement(593..2125)
                     /gene="gpmI"
                     /locus_tag="VK031_07305"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HLS53763.1"
                     /translation="MIKKPVMLIILDGWGIGDNYPGNAIEIGNTPNFDRLMKAYPNTS
                     LEASGLAVGLPEGQMGNSEVGHLNIGAGRVVFQELPRITKAIEEGEFFKKKEFLAAVE
                     NAKKNNSKLHLIGLVSDGGVHSHMKHLYGLLELMKKEELDQVYIHAILDGRDVPPTVG
                     ASHIEELNNKTKQLGVGKIATVSGRYYTMDRDRRWERTQLAYDAMTQAKGQTAQDPVA
                     AVKSSYEEGVTDEFMLPTIIMEEDKPVATVEDGDSIIFFNFRPDRARQITRAFVDENF
                     QGFEREKKVNTFYVTMTEYDKTIENVHVAYRSEAPENTLSQYISSKGLNQLKIAETEK
                     YAHVTFFLNGGIEEPYENEDRVLIPSPKVATYDLQPEMSAIKVKDEVLDRLNMDKYDL
                     IVVNFANPDMVGHTGVIPAVKKAVETVDSCLGEIIKLLEEKGGKALVTADHGNAEKLL
                     DKEGNPVTAHTTNRVPLILVGEEKAGLKEGKLADLAPTLLDLMGLDKPEEMTGKSLLV
                     KE"
     gene            complement(2140..2886)
                     /gene="tpiA"
                     /locus_tag="VK031_07310"
     CDS             complement(2140..2886)
                     /gene="tpiA"
                     /locus_tag="VK031_07310"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HLS53764.1"
                     /translation="MRTPIIAGNWKMNKTIAEAIDLVEGIKDHELNKEVEAVLCVPFT
                     DLRDVKKTLKGTNIKLGAQNMHWEESGAYTGEISPLMLKELGIDYVILGHSERRQYFN
                     ESDEIVNKKMKAALKHNLKPILCVGESLEERESNKAEDVVKEQITKAFNGINKEEIEN
                     IVIAYEPIWAIGTGKTASADDANNMIKFIRNIIGKLYGAEEQEKIRIQYGGSVNPGNV
                     KELMAKSDIDGALVGGASLKAEDFVQLINY"
     gene            complement(2899..4092)
                     /locus_tag="VK031_07315"
     CDS             complement(2899..4092)
                     /locus_tag="VK031_07315"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HLS53765.1"
                     /translation="MLDKKTLKDLNFQGKRVLVRCDFNVPMDEEQNITDDTRITSSLP
                     TINYLIEEGAKVILMSHLGRPKGEAKPEFSLEPVAKRLSQLLKKEVVFARDDQVVSAN
                     VKNQIAQLEDGDVALLENTRYRLEETENGEDFAKDLASLGEIFVNDAFGTCHRAHASN
                     VGVSSILPSAVGFLVEKEISIMGKALEDAERPFVSILGGAKVSDKIGVIENLLDKVDT
                     ILIGGGMAYTFLKAQGYEIGQSLLEEDKIDLAKELLDKAKSKGVELLLPVDVVAAKEF
                     KNDTEIKTVDIDKIPADMMGLDIGPKTIDLFSDVIAKAKTVIWNGPTGVFEMENFSKG
                     TYAVAKAMAESAATTIVGGGDSAAAVEKAGYKDKISHVSTGGGASLELLEGKILPGID
                     AISNR"
     gene            complement(4150..>5069)
                     /gene="gap"
                     /locus_tag="VK031_07320"
     CDS             complement(4150..>5069)
                     /gene="gap"
                     /locus_tag="VK031_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HLS53766.1"
                     /translation="DLVAINASGDLNDLAHLLKYDTMYGKFDGTIEVEEDGFVINGKK
                     VKVVAHRDPLEIPWKELGVELVVDSTGAFRDREGLSKHLEAGAKKVIVTAPAKDEDIT
                     IVLGVNEDLYDADKHNIISNASCTTNCLAPVAKVVSDKLGIKKGLMTTIHAYTNDQQI
                     LDKRHKDLRRARAAAANIVPTTTGAAKAVALVLPELEGKLNGFSVRVPVPTVSMVDVV
                     FEVEKSTTAEEVNKLFKEASENELKGVLGFSEEPLVSSDYVGDPRSSIVDGLSTMVID
                     NMVKVVAWYDNEWGYSERVIDLARLVATK"
BASE COUNT         1253 a         1199 c          771 g         1846 t
ORIGIN      
        1 aggcataatc ataaattctt gtatatccac gttattgtcg gcatgctctc caccgtttaa
       61 tatattcatc attggaaccg gcaattgttt ggcattgatt ccaccaatat attgatacag
      121 tggcattcct aaagcatcag ctgctgctct agccacagca acagacacac ctaagatggc
      181 atttgctcct aatttcccct tgtttggtgt gccatctaaa tctattagta gctggtcaat
      241 agccaattgg tcgaatacgt ccattcctat tatttcatct gctattattt catttacatt
      301 ttttactgct tttaagaccc ctttacccaa atacctggat ttgtctccat cccttaattc
      361 aacggcttca aaggctccag ttgatgctcc agaaggtacc aatgctctac caaaggctcc
      421 actttcagta actacttcta cctctactgt tggattggcc cttgaatcta atacctctct
      481 tgcatataca tcaattatca tactcaagct tatcatcctt tctttgaata tatttatctt
      541 tatttgtttg tctaagatta cttagattat ttataactaa taatttacct atctattctt
      601 tgactagcag ggatttacct gtcatctcct ccggcttatc tagccccata aggtccagta
      661 aggtaggtgc caaatcagcc aatttacctt cctttaggcc ggccttttct tctcctacca
      721 aaattaaagg aactctattg gtggtatggg ctgtaaccgg attgccttct ttatctaaca
      781 atttctctgc attcccatgg tctgcagtaa ccaaggcttt gccgcccttt tcctctaaca
      841 attttataat ctcacctaaa catgagtcta ctgtttcaac tgccttttta acagcaggaa
      901 ttactcctgt atgacctacc atatctggat tggcaaagtt taccacaatt agatcatatt
      961 tatccatgtt taatctatct aaaacctcat ctttcacctt aatagcactc atctctggtt
     1021 gcaaatcata tgtggctacc ttaggcgaag gtataagcac cctatcttca ttttcataag
     1081 gctcttctat tccaccattt aagaaaaagg ttacatgggc atatttttct gtttctgcaa
     1141 tttttaattg atttaatccc ttagaactta tatattggga caaagtattt tctggggctt
     1201 ctgatctata ggccacatga acattttcta tggttttgtc atattcagtc atggttacat
     1261 agaaggtatt taccttcttt tccctttcaa atccttggaa attttcatct acaaaggccc
     1321 tagtaatttg tcttgcccta tcaggcctaa aattgaaaaa tataatactg tccccatctt
     1381 ctactgtggc cacaggctta tcttcttcca taataattgt tggaagcata aactcatctg
     1441 tgaccccttc ttcataggaa ctttttactg ctgccactgg atcctgggca gtctgtccct
     1501 tagcctgggt cattgcatca taggccagtt gtgtcctttc ccatcttcta tccctatcca
     1561 tagtatagta tctacctgat actgttgcta tctttcctac ccctaactgc ttggtcttat
     1621 tatttaactc ttcaatatgg cttgctccta cagtaggcgg tacgtctcta ccatccagga
     1681 tggcatgtat ataaacctga tctaattcct cttttttcat cagctctaaa agtccatata
     1741 ggtgtttcat atggctatga accccaccgt ctgaaactaa gccaatcagg tgtaattttg
     1801 aattattctt ctttgcattt tcaacagctg ctaggaactc tttcttttta aagaattctc
     1861 cttcttctat ggccttggta atccttggca attcttgaaa aactactctg cctgcgccaa
     1921 tatttaaatg gcctacctca gaattgccca tttgtccttc tggtaatcct actgccagtc
     1981 ctgaagcttc aaggctggta ttagggtagg ctttcatcag tctgtcgaaa tttggtgtgt
     2041 ttccaatttc tattgcattt cctgggtaat tatcaccaat tccccagcca tctagtatga
     2101 ttaacattac tggtttcttt atcatcaata ctcctccagt taatagttta taagctgtac
     2161 gaaatcctcc gctttaaggc tggctcctcc aactaaggcc ccatcgatat ctgacttggc
     2221 catcaattcc tttacattgc ctggatttac actgcctcca tactggattc ttattttttc
     2281 ctgttcttca gctccataaa gtttgcctat aatatttcta ataaacttaa tcatattgtt
     2341 ggcatcatct gctgaagcag ttttccctgt ccctatggcc cagataggct cgtaagctat
     2401 aactatattt tctatctctt ccttatttat cccattaaag gctttggtta tctgttcctt
     2461 gacaacatcc tctgccttgt ttgactctct ctcttccagg ctttccccta cacaaagtat
     2521 gggttttaga ttgtgtttta gggctgcttt catcttttta tttacaatct catcgctttc
     2581 attaaaatat tgtcttcttt ctgaatgtcc taagatcaca taatctattc ctaattcctt
     2641 tagcattaag ggagagattt ctcctgtata ggctccgctt tcctcccagt gcatattttg
     2701 tgctcctagt ttaatattag tgccctttaa ggtcttttta acatccctca ggtctgtaaa
     2761 gggtacacat aaaactgctt ctacctcttt atttaactca tggtccttaa tgccttctac
     2821 taaatcaatt gcctctgcaa ttgtcttgtt cattttccaa tttcccgcga ttataggagt
     2881 tcgcataatt tacctccttt acctattaga tatggcatct atacctggta atatctttcc
     2941 ttctaataat tctaaagaag ctcctccgcc tgtagacaca tggctgattt tatctttata
     3001 gccggccttc tcaactgcag cagcagagtc tcctccacct actatggtag tagctgctga
     3061 ctcagccatg gccttggcta cagcgtaggt tcctttactg aagttttcca tttcaaagac
     3121 tcctgtcggt ccattccaga taacagtttt agccttggct atcacatcag aaaataggtc
     3181 tatagtttta ggtccaatgt caagtcccat catatctgca ggtatcttat ctatatctac
     3241 agtttttatt tcagtgtcat ttttaaactc cttggctgcc accacatcta ctggcagtaa
     3301 aagctctact cctttagatt tagccttatc taaaagctct tttgctagat ctattttatc
     3361 ctcctctaat agagactggc caatttcata gccttgggcc tttaaaaatg tataggccat
     3421 tcctccacct ataaggatgg tatctacctt gtctagtagg ttttctatta ctcctatttt
     3481 atctgaaacc ttggctccac ctaagattga tacaaagggt ctttcagcat cttctaaggc
     3541 cttgcccatt atagagattt ccttttccac taaaaagccc actgctgatg gcaatataga
     3601 agatactccc acattagatg catgggccct atggcaggtt ccaaaggcat catttacaaa
     3661 aatttcacct aaagatgcta aatcttttgc aaaatcttcg ccgttttcag tttcctccag
     3721 cctgtatctt gtgttttcca acagggctac atctccatct tccagttgtg caatctgatt
     3781 tttaacattt gcagaaacca cctgatcgtc tcttgcaaag actacctcct tctttaataa
     3841 ctgggatagc cttttggcaa ctggctctag gctaaattct ggtttggcct ctccctttgg
     3901 tcttcccaga tgtgacataa ggattacttt tgcgccctct tctattaaat aatttatagt
     3961 tggtagtgaa cttgtgattc ttgtatcgtc agtaatattt tgttcctcat ccataggtac
     4021 attaaagtca catctaacta agactctttt accttggaag tttaaatcct tcaatgtttt
     4081 tttatctagc atattaccac ctcacctaac attagtagtc gggtcttaaa aaagacccga
     4141 cttttatatt tacttagtag ctactaatct tgcaaggtct ataactctct cagagtagcc
     4201 ccactcgttg tcataccaag ctacaacttt aaccatgtta tctattacca tagttgatag
     4261 gccatctact attgaggatc ttggatctcc tacatagtct gaggatacta atggctcttc
     4321 tgagaatcct agtactcctt ttagttcatt ttcgcttgct tctttaaata gtttatttac
     4381 ttcttcagca gtagttgatt tttctacttc aaataccaca tctaccatgg atactgtagg
     4441 aactggtacc cttactgaga aaccatttag ttttccttcc aattctggca atactaatgc
     4501 tactgcctta gcagcacctg tagttgttgg cactatgtta gctgcagctg ctctagcacg
     4561 tcttaaatct ttgtgtctct tatctagaat ttgttggtca tttgtataag catgaattgt
     4621 agtcattaga cctttcttta tgcctaattt atctgatact acctttgcta ctggagccaa
     4681 gcagtttgtt gtacaggatg cgttggatat aatgttatgc ttatctgcat catataggtc
     4741 ttcatttact ccaagtacta ttgtaatgtc ttcatctttg gctggtgctg ttactataac
     4801 tttctttgct ccagcttcta agtgcttgga taggccttct ctatctctga aagcacctgt
     4861 tgagtctact actaactcta cacctagttc tttccatgga atttctaatg gatccctgtg
     4921 ggcaacaact ttaaccttct tgccgtttat tacaaaaccg tcttcttcca cctctattgt
     4981 tccatcaaac tttccataca tagtgtcgta ttttaatagg tgggccagat cattcagatc
     5041 tcctgaagca ttaatcgcaa ctaggtcaa
//
LOCUS       DATGSI010000473         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_30998, whole genome shotgun sequence.
ACCESSION   DATGSI010000473 DATGSI010000000
VERSION     DATGSI010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30998"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VK031_07325"
     CDS             complement(<1..1191)
                     /locus_tag="VK031_07325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002834787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HLS53767.1"
                     /translation="MNKERGHNDMTIDSFIKSVQDIMRQDAGVDGDAQRISQLIWMLF
                     LKVYDAKEAEWELFEEDYKSIIPEDLRWRNWAEDEEGMTGDELLEFVNERLFKELKDL
                     TVDETTDKRALIVKSVFEDSYNYMKSGTLLRQVINKLNELDFEDYEDRHAINDMYETI
                     LRDLQSAGNAGEYYTPRVLTDFIIEMLNPQIGERIADFACGTGGFLVSALKHLEKQIK
                     TVEDRRIMQSSLFGIEKKPLPHLLCMTNMLLHDIDSPNILRDNSLSTNVRDYTEKDKY
                     DVIAMNPPFGGVEEDGILINFPTEFRTSETADLFMTLIMYRLKETGRAGVILPDGFLF
                     GDDNKKLAIKKKLLSEFNLHTIVRLPNGVFAPYTPIATNVLFFDKSKPTEKIDYYQVP
                     LPEGK"
     gene            complement(1218..>2453)
                     /locus_tag="VK031_07330"
     CDS             complement(1218..>2453)
                     /locus_tag="VK031_07330"
                     /inference="COORDINATES: protein motif:HMM:NF020052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HLS53768.1"
                     /translation="KVDKYGNLIEDRIYNVSDFDRNLILEKRTEIVAKKITEFLKKTN
                     RFDKTIVFCVDIDHAERMRQALINENSDLVKGNDKYIMKITGDDEVGKAQLDNFIDPA
                     SKYPTIVTTSKLLTTGVDAQTCKLIVLDSNINSMTEFKQIIGRGTRIKEEYGKLFFTI
                     MDFRGVTRLFADPDFDGDPVVIYEPKEGEDIVPPTEREYDDFEGEPGAYVNEDEGDFD
                     LDENGGGKRVKYVVDDVEVMVVNERVEYYDKDGKLVTESLKDYTRKNILNEYADLNEF
                     INRWNSEEKKSAIIEELKERGVFLDAIKEEVGKDLDDFDLILHLAFDQKPLTRQERAN
                     NVKKRDYFSKYSDVAREVLEALLDKYMNEGITELESMEILKLDPFKKIGTPTKIIKAF
                     GGRDKYIQTIKELEREIYA"
BASE COUNT          692 a          500 c          289 g          972 t
ORIGIN      
        1 tttaccttct ggtaatggca cttgatagta gtctatcttc tcagtaggtt tggatttatc
       61 aaagaataat acattggtag caataggggt atatggtgca aatactccat tcggcaatct
      121 tacaatagta tgtagattga attcacttag tagtttcttt ttgattgcaa gctttttatt
      181 atcatctcca aataaaaatc catcaggtag tattacacct gctctgcctg tttcttttaa
      241 tctatacata ataagagtca tgaatagatc tgcagtttct gatgttctaa attctgttgg
      301 aaaattaatt aaaataccat cttcctcaac ccctccaaaa ggtggattca tggcaattac
      361 atcatattta tccttttctg tatagtccct tacgttagtt gaaagggaat tatctcttaa
      421 gatatttgga ctatctatat catgtaataa catatttgtc atacataaca aatgagggag
      481 tggctttttt tctattccaa atagtgaaga ttgcattatt cttctatctt ctacagtttt
      541 aatttgtttt tctaaatgtt ttaaagcaga tactaaaaag cctcctgtac cacaggcaaa
      601 gtctgctatt ctttctccta tttgaggatt taacatctca attataaaat ctgtcaaaac
      661 ccttggtgta tagtattcac cagcattacc agcactttgc aagtctctta gtattgtttc
      721 atacatatca ttaatagcat gtctatcttc ataatcctca aaatctaatt cgtttaattt
      781 atttataacc tgacgaagaa gggttcctga tttcatataa ttatagctat cttcaaatac
      841 agatttaaca attaaagctc ttttatctgt agtttcatct actgttaaat cttttaactc
      901 cttgaacaac ctttcattta caaattctaa tagttcatct cctgtcattc cttcttcatc
      961 ttctgcccag tttctccatc ttaaatcttc aggaataatt gacttatagt cttcttcaaa
     1021 tagttcccat tcagcttcct ttgcatcata aacctttaaa aaaagcatcc agataagttg
     1081 ggatatcctt tgagcatctc catctactcc tgcgtcttgt ctcattatat cttgtactga
     1141 ttttataaat gaatcaatgg tcatatcatt atgtcccctt tctttattca agtaaaatgc
     1201 tccttataaa ggagcgtcta tgcatatatt tctctttcta attctttaat tgtttgaata
     1261 tacttatctc tcccaccaaa ggcttttatt atctttgtag gagttcctat ttttttaaat
     1321 gggtctagct taaggatttc catactttct aattctgtta ttccttcatt catatattta
     1381 tctaaaagag cttctaacac ttcccttgcc acatctgaat atttactaaa atagtctctc
     1441 ttcttaacat tattagctct ttcttgtctt gtaagtggct tttggtcaaa ggctaaatga
     1501 agtattaaat caaagtcatc tagatctttt cctacctcct cttttatcgc atctagaaat
     1561 actcctcttt ccttaagctc ttcaataata gctgattttt tctcttcact attccatcta
     1621 ttaatgaatt catttaagtc tgcatattca tttagaatat tctttcttgt ataatccttt
     1681 aatgattcag taactaactt accatcctta tcatagtatt cgaccctttc atttacaacc
     1741 atgacttcca catcatctac tacatattta actcttttac ctccaccatt ttcatccaag
     1801 tcgaagtctc cctcatcttc attaacataa gctccaggct ctccttcaaa atcatcatat
     1861 tctctttcag tcggtggaac tatgtcttct ccttctttgg gttcatatat aactacaggg
     1921 tcaccatcaa aatctgggtc tgcaaagagc cttgttactc ccctaaaatc cataatagta
     1981 aaaaataatt tcccatactc ttccttaatc cttgtgcccc tacctataat ttgcttaaac
     2041 tctgtcatac tgtttatgtt gctatcaagg actataagtt tacaagtttg agcgtctact
     2101 cctgtggtca aaagtttaga tgttgttact atagttggat atttacttgc aggatctata
     2161 aagttgtcta attgtgcttt acctacttca tcgtctccag ttatcttcat aatatactta
     2221 tcatttcctt ttactaaatc cgaattctca tttataaggg cttgtcgcat tctttctgca
     2281 tggtctatat caacacagaa aacaatggtt ttatcaaatc gattagtctt ctttaagaat
     2341 tcagtaattt tctttgctac tatttccgtc cttttttcaa gtattagatt tctatcaaaa
     2401 tctgatacat tatatatcct atcttctata aggtttccat acttatctac ttt
//
LOCUS       DATGSI010000474         1060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_175918, whole genome shotgun sequence.
ACCESSION   DATGSI010000474 DATGSI010000000
VERSION     DATGSI010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175918"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VK031_07335"
     CDS             complement(<1..916)
                     /locus_tag="VK031_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004837338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HLS53769.1"
                     /translation="MVLLFVGIAILLIGGVLYSKYVESQFQPDDRPTPAVKKADGVDY
                     VTMNKKKNWLINLLNIAGTGPILGPIQGILFGPIAFLAIPLGCVFAGAVHDYFTGMIS
                     IRNGGSQVPKLVGKYLGKNVRRFYNIIIAILLLLTGTVFVYTPGDLIANDILGLDPNS
                     NVIWIIYGAIFLYYILSTVLPIDKLIGRIYPLFGAFLLISSLGVFIGIIYKGLPHLQF
                     EGYGLLYDHPTGQRFIPSFFITVACGILSGFHGSQVTLVSRTVGSEKEGIGTFYSTMI
                     AEGFIAMVWAAGALVLFSTGMAALDTAGT"
BASE COUNT          365 a          192 c          162 g          341 t
ORIGIN      
        1 gtgttcctgc tgtatcaagg gctgccatgc ctgttgaaaa caataccaag gctccagctg
       61 cccaaaccat agcaataaaa ccttctgcaa tcatagttga gtaaaatgtt cctatacctt
      121 ctttttcact tcccactgtt cttgaaacaa gggttacttg tgagccatgg aatcctgaaa
      181 gaataccaca agctacagtt ataaagaaag atgggataaa tctttgacca gttggatggt
      241 catataaaag cccatatcct tcaaattgta agtgtggaag tcccttatag attattccta
      301 taaatacacc caaggatgaa ataagtaaaa aggctccaaa tagtggataa attcttccta
      361 ttagcttatc tattggaagt actgttgata atatgtaata aagaaaaata gcaccatata
      421 taatccaaat tacatttgaa ttaggatcaa gtcctagaat atcatttgca attaagtctc
      481 ctggtgtata aacaaatact gttcctgtaa gaagtagtaa aattgcaata attatattat
      541 aaaatcttct tacatttttt ccaagatact ttccaacaag tttaggaact tggcttcccc
      601 catttcttat ggaaatcata cctgtaaagt aatcatgtac cgcacctgca aatacacaac
      661 ctaggggtat ggccaaaaag gctataggtc caaacaaaat tccttggatt ggtccaagaa
      721 ttgggcctgt accagctata tttaataggt ttattagcca gtttttcttt ttgttcatag
      781 taacatagtc gacaccgtcc gcttttttta ctgcaggagt gggtctgtca tctggttgaa
      841 attgactttc aacatatttt gaataaagga ctcctcctat aagtaggata gcgattccta
      901 caaaaagtaa taccatcata acctccttta ttataatgtc tacacttttg attataacac
      961 ttttaacaaa aaaattcaat attaatagat tatagttaat atattatttt ttgtaaaaaa
     1021 atataggctt ttagatttaa ataaaaaata accaggccct
//
LOCUS       DATGSI010000475         5784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_80056, whole genome shotgun sequence.
ACCESSION   DATGSI010000475 DATGSI010000000
VERSION     DATGSI010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80056"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VK031_07340"
     CDS             <1..480
                     /locus_tag="VK031_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLS53770.1"
                     /translation="VLDPYLVILNSLPKTALAPIIIIWVGAGYSGIIVTAISISIVVT
                     IMNISLSFRSVDEEKIRLLETFGASKTQILTKLILPSSVPSIISTLKVSIGLSWVGVI
                     VGEFLVSKAGIGYLIVYGGQVFRLDVVMMGVVVLALISALMYKVINLVEEKFTKWIQ"
     gene            complement(501..1106)
                     /locus_tag="VK031_07345"
     CDS             complement(501..1106)
                     /locus_tag="VK031_07345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLS53771.1"
                     /translation="MSLEQLKPPKAGEEIAIIKTSLGHIKIRLFPQIAPKAVENFKVH
                     AQNKYYDGLLFHRVIHDFMIQGGDPEGSGRGGESIWGQAFEDEFHLYYRNFRGALSMA
                     NSGPNTNGSQFFIVQKQTRDKKIIEQMRNMGKDRGYPEEVVDIYEKYGGSYWLDFKHT
                     VFGQVFQGMEVVDQIARLKTDDRDRPLEPVLMEKIRIIKYK"
     gene            complement(1119..1808)
                     /locus_tag="VK031_07350"
     CDS             complement(1119..1808)
                     /locus_tag="VK031_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HLS53772.1"
                     /translation="MLEYWIIITRLLLSAILGGLIGLEREASNRPAGFRTHILVTIGS
                     TLIMLVSVDSFHGFADPSRIAAQVVSGIGFLGAGTILRTGNNIEGLTTAASLWVCAGI
                     GLAIGNGYYLAAIATAIIVLFFLKNIGFFERRVLRKTLGHFQFVCQVRAGLIGDIGTI
                     LGEHHVIIKDINILGIDLGDKVKEEINLRVKLPSKADLDKLFIELAAVKGMYKILYNG
                     EEIKETGQLVR"
     gene            complement(1834..3198)
                     /locus_tag="VK031_07355"
     CDS             complement(1834..3198)
                     /locus_tag="VK031_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HLS53773.1"
                     /translation="MDRNIDLTKGNIFKTLVKLALPIMGTSFIHMAYNLTDIMWLGRL
                     STEAVAAAGTAGFFMWFGSGLILMSQIGVGVEVAQAYGRNDIDEARKYISNGIQLNII
                     MAMVYGIILIIFRHLIIGFFKLTDINVYNMSIEYLIIISIGMVFNFINPVLSTILNSS
                     GDSMTPFKVNAVGLVFNMLLDPLFIFGLGPIKGMGIKGAAIATVLSQVVVTLIFLSIG
                     KKNQTLYFQAKLFNKLEKAYILRIVKLGFPAFLQTSAHSSISMVLTRIIAKFGPVPIA
                     VQSVGSKIESISWMTAEGFSAAISAFMGQNYGAKKYERIREGYKKGMQIVGSIGIFAT
                     LLLILAAEPLFAIFVPEDPMAIAEGARYLRILGYSQFFMSIEIGTAGAFNGLGRTLPP
                     AIVGVSLNALRIPVAIYLSTYTALGLSGVWWSISSTSIVKGILLATLFITMLRRRFPI
                     EENI"
     gene            complement(3360..4589)
                     /locus_tag="VK031_07360"
     CDS             complement(3360..4589)
                     /locus_tag="VK031_07360"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HLS53774.1"
                     /translation="MSRTRNNLTAKIFALIIAIFLWSYVMSEVNPEIPKEIRNVDVTL
                     KNTDLLKRQGLVVMEPKDFKVNVRIAGKKSDIDRFLSKGSGNILAQADLAGYGEGQHR
                     VPVTISLLDNSSNIRVISWEPREILFTFDKIITKEKTVSIKTEGEVGENYVVDSITAK
                     PQTILIKGPRTWVNQVAEARVLVNMKDRTSSTSLTLPVQLLDDQENDVSDLEKDPGTV
                     DIDIKILRKKTLPIRLQIEGDIPDNYNIEDIKVYPSTITVKGDNSIEGLTHINTKPIK
                     IQDLLDGSFDGVELDLPENVQLLDPQQKITLNYSVEEIIEKEYEIDFDNVEIRNLEEG
                     LSLEDESPGSISVKLKGPKSSLEKILEDNLKLYVDLKGLKEGKHEVDVKIEGITDITV
                     EYIMPDKIEIDLIKEEE"
     gene            complement(4570..5409)
                     /gene="cdaA"
                     /locus_tag="VK031_07365"
     CDS             complement(4570..5409)
                     /gene="cdaA"
                     /locus_tag="VK031_07365"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HLS53775.1"
                     /translation="MSLQQIISTFLAIRMRDVIDIVIVAVVVYKLFTLIKETRAEQLT
                     KGIIMLLILAKLTEWAQLYTINWILNNAMTVGTLAILIVFQPELRRGLEYIGRSSFLT
                     KSFLEIRGESLSKIVEELVDAVASLSRQKIGALIVIERQTGLNEVAETGTLIEGEVTS
                     DLLINIFIPNTPLHDGAVIIKDDKIKAAGCFLPLTDNTGISKELGTRHRAALGISERS
                     DSISIVVSEETGGISIAESGAISRYVDIKTLRQILLDMYKPEENKQTFMSRWRLRNEQ
                     DKE"
     gene            complement(5502..>5784)
                     /locus_tag="VK031_07370"
     CDS             complement(5502..>5784)
                     /locus_tag="VK031_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53776.1"
                     /translation="NQRMETMGNSYAEDGVMVRFSIEKDLNTEETMIRKVEFIPTWVH
                     KYIEKNKTHYKILPVEEVIEEKLDIKLSPSVEERIEKSLIDTFNTLDPK"
BASE COUNT         1636 a         1233 c          859 g         2056 t
ORIGIN      
        1 gttttggatc cttatttggt catattaaat agcctaccta aaactgcttt ggctcctatt
       61 ataatcatat gggtaggggc aggttattct gggataatag ttactgcaat ttctatatct
      121 attgtggtta ctattatgaa tataagtttg agttttcgct ctgtagatga agaaaaaata
      181 agacttttgg agaccttcgg agccagcaag actcaaattc taacaaaatt aatcctgcct
      241 tcttccgttc ccagtatcat aagtacttta aaggtaagta ttggcctatc ttgggttgga
      301 gtgattgtag gtgaattttt agtatccaag gctggcattg gttacttgat cgtttatggt
      361 ggccaagtat ttaggctaga tgtggtaatg atgggagtag tggtcctggc cttgatttct
      421 gccctaatgt ataaggtaat caatttagta gaagaaaagt ttaccaaatg gatacaataa
      481 aagtctccta agggagactt ttacttatat tttataattc ttatcttttc catgaggacc
      541 ggttctaagg gtctgtctct gtcatcagtt tttaatctag caatctgatc caccacttcc
      601 atcccctgaa aaacctgtcc aaatactgta tgcttaaaat ccaaccaata acttcctcca
      661 tatttttcat aaatgtctac tacttcttct ggatatcccc tatccttgcc catatttctc
      721 atctgctcaa ttatcttttt atctcttgtt tgcttttgga ctatgaaaaa ctggctgcca
      781 ttggtattag gtcctgaatt agccatagat agggcccccc taaagtttct ataataaaga
      841 tggaattcat cttcaaaggc ctgcccccaa atgctttccc cccctctacc gctaccttct
      901 ggatctccgc cctggatcat aaagtcatgg attaccctgt ggaaaagaag gccatcataa
      961 tatttgtttt gtgcatgaac tttgaagttt tccacagcct ttggtgctat ctggggaaaa
     1021 agcctgattt taatatgtcc aaggctagtc tttattatgg caatttcctc tcctgccttc
     1081 ggtggcttca attgttctaa actcaagttg ctccctcctt accttactaa ttgtcctgtt
     1141 tccttaatct cttctccatt atataggatt ttgtacatgc cctttactgc agctagttct
     1201 ataaataatt tatctagatc agccttagag ggcagtttta ctctaagatt aatctcttcc
     1261 ttaaccttat ctcccaggtc tattcccaat atatttatat ccttaattat tacatggtgt
     1321 tcacctaaaa ttgtaccaat atctcctatt aggcctgccc ttacctggca aacaaattgg
     1381 aagtgtccta aggtttttct aaggaccctc ctttcaaaga aacctatatt tttcagaaaa
     1441 aagagtacaa ttatggcagt ggcgattgcg gccaagtagt agccattacc aattgccaat
     1501 cctattcctg cacataccca taggctggct gctgtagtta acccctctat attatttcct
     1561 gttcttaata tggtacccgc tcctaaaaat cctattccgc ttacaacctg tgcggcaatc
     1621 cgggaaggat cagcaaagcc atgaaaacta tctacagaaa ctagcattat tagtgtagag
     1681 cctatagtca ccaggatatg tgttctaaat cctgccggcc tattactagc ctctctttct
     1741 aaaccaatca ggccacctaa tatggctgat aataaaagcc tagtaattat aatccaatac
     1801 tctaacataa ccttacctcc ctatactggg caactatata ttttcttcaa tgggaaatct
     1861 tctccttaac attgttataa acaaggtggc taatagaatg ccctttacaa tgctggtgct
     1921 actaatactc caccatactc ctgatagtcc taaggctgta taagtagata aataaattgc
     1981 cacaggtatt cttaaagcat ttaaactgac cccaactatg gcaggaggta gtgtacgtcc
     2041 aagaccattg aaagctccag cagtacctat ttctatactc ataaaaaatt gggaataacc
     2101 cagtattctt aaatatctag ccccttcagc tatagccata ggatcctccg gtacaaagat
     2161 ggcaaatagt ggttctgccg ccaatattaa taacaaagta gcaaatatgc ctatactgcc
     2221 tactatttgc attccctttt tataaccttc cctaattctt tcatactttt tggctccata
     2281 attttgaccc ataaaggctg atatggctgc tgagaagccc tctgccgtca tccaggatat
     2341 ggactctatt ttagagccta ctgattggac tgcaatggga acaggaccaa atttagctat
     2401 gattcttgtt aagaccatac ttatgctaga atgggctgag gtttgtaaaa aggcaggaaa
     2461 gcctagttta actattctta ggatataggc cttttccagc ttattaaata attttgcttg
     2521 gaaataaagg gtctgatttt tcttgcctat tgataaaaat ataagagtta ccacaacttg
     2581 ggaaagtact gtagctatag ctgccccttt tattcccatg cccttgattg gtcccagacc
     2641 aaatatgaat aagggatcca ataacatatt aaacaccagg ccaactgcat taaccttaaa
     2701 aggagtcatg ctatctcctg aactatttaa tatggtagaa agtactggat ttataaaatt
     2761 aaaaaccata cctatagata tgataatcag gtattcaata gacatgttat aaacattaat
     2821 atctgtcagt ttgaaaaagc ctatgatcaa atgtctaaaa ataattaata taattccata
     2881 gaccatggcc attattatat ttaattgaat tccattggaa atatatttcc ttgcctcatc
     2941 tatatcattt ctcccgtagg cctgggccac ctcaactcct actcctatct gtgacataag
     3001 gattagcccc gatccaaacc acataaaaaa acctgcagtt cctgctgcgg ccacagcctc
     3061 tgtacttaac ctacctagcc acataatatc agtcaggtta taggccatat gtataaaaga
     3121 tgtacccatt ataggtaacg ctagtttgac caatgtttta aatatatttc cctttgttaa
     3181 atctatattc ctatccattc tgtctccctc tactgtattt ttttctttat tgctttagac
     3241 aattttatat tattagtagt atttaatcaa gattttttat tttctccagt ttaatcttaa
     3301 aaaggaaaat cccttacaac ttgggatttt cccttttatc ttcctttcat ttaacctagt
     3361 tattcttcct cttttattaa gtcaatttct attttatctg gcataatata ttcaacagtt
     3421 atatcagtta taccttcaat ttttacatct acttcatgct tgccttcttt caagcctttt
     3481 aagtctacat aaagctttaa attatcctct agaatttttt ccaagcttga tttaggaccc
     3541 tttaacttta cagatataga tccagggctc tcatcttcta ggcttaagcc ctcttctaaa
     3601 tttctgatct ccacattatc aaaatcaatt tcatattcct tctctataat ttcttccact
     3661 gaatagttta gggttatctt ctgctgggga tccaatagtt gaacattttc tggcaaatcc
     3721 aattcaaccc catcaaagct tccatccaat aggtcttgga tctttatagg ctttgtatta
     3781 atatgggtta ggccttctat actattgtca ccttttactg ttattgtact tggataaacc
     3841 tttatgtctt ctatattata attgtcaggt atgtcacctt ctatttgtaa tctaataggc
     3901 agggtctttt tcctcagtat ctttatatca atatccacag ttcctgggtc tttttccaaa
     3961 tcagatacat cattttcttg atcatccaat agctggactg gtaatgttaa gctagtggaa
     4021 gaagtcctat ccttcatatt aaccaaaact ctggcttctg ccacctggtt tacccaggtc
     4081 ctaggtccct ttataaggat tgtttgtggc tttgcagtaa tactatcaac cacataattt
     4141 tctcctactt ctccctctgt ttttatgcta actgttttct ctttagtaat aatcttatca
     4201 aaggtaaaaa gaatctccct tggttcccag cttattaccc gaatattaga tgaattatct
     4261 aaaagactga tagttacagg taccctgtgt tgaccttctc catagcctgc caggtctgcc
     4321 tgggccaata tatttccaga acctttagat agaaacctat ctatgtcaga cttttttccg
     4381 gctattctca cattaacttt aaagtcttta ggttccatga ctactaagcc ttgccttttt
     4441 aacaggtctg tatttttcaa ggtcacatcc acattcctaa tctctttagg gatttcagga
     4501 tttacctcac tcattacata actccataag aatatggcga ttattaaagc gaaaatttta
     4561 gctgttaaat tattccttgt cctgctcatt tcttagcctc catctagaca taaatgtctg
     4621 cttgttttcc tcaggcttat acatatctaa taagatttgt cttaaggttt ttatatctac
     4681 atacctagag attgcaccgc tctctgcaat agatatgcca ccagtttcct cagaaaccac
     4741 tattgaaata ctgtctgatc tttctgaaat tccaagggct gccctgtgcc tagtacctaa
     4801 ttctttactt atgcctgtat tgtctgttaa aggcaaaaaa catccggctg cctttatttt
     4861 atcatccttg attatgactg ccccatcgtg taaaggtgta ttaggaatga aaatatttat
     4921 aagtagatca ctggttacct ctccttctat taaagtgcct gtttctgcca cctcatttag
     4981 tcctgtttgc ctttctatta ctattagggc tcctattttt tgtctagaca aggatgctac
     5041 tgcatctacc agttcttcta ctatctttga caggctttcc cctctgattt ctaaaaagga
     5101 tttagtaaga aagctacttc ttcctatata ctctaaacct ctacgtaatt ctggctgaaa
     5161 aactattaat atggctaaag tacctacagt catagcatta tttaaaatcc aattgattgt
     5221 atataattgg gcccattccg ttaatttggc taggattaga agcattataa ttcccttagt
     5281 caactgctct gccctagttt ctttgattaa tgtaaataat ttatatacaa caacagcaac
     5341 tattactata tctattacat cgcgcattct tatagccaaa aatgtactta taatttgttg
     5401 caaagaaatc acacctttaa aataattgtt gtgcgaacct atatttcact actactccta
     5461 ttatataata aaggctttag gattaaaatc cttttaactc tttatttagg atctagggta
     5521 ttaaaagtat caattaaaga cttttctatc ctttcttcca cactggggct taattttata
     5581 tctaattttt cctctattac ttcttctact ggcagtattt tataatgggt tttattcttt
     5641 tcaatatact tatgaaccca ggttggtata aattccactt tacggatcat tgtttcctca
     5701 gtattaagat ccttttctat agaaaatcga accattaccc catcttctgc ataggaattt
     5761 cccatagttt ccatccgttg gtta
//
LOCUS       DATGSI010000476         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_314239, whole genome shotgun sequence.
ACCESSION   DATGSI010000476 DATGSI010000000
VERSION     DATGSI010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314239"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1656
                     /gene="valS"
                     /locus_tag="VK031_07375"
     CDS             <1..1656
                     /gene="valS"
                     /locus_tag="VK031_07375"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HLS53777.1"
                     /translation="QVKEHENAVGHCERCRTVIEPIISKQWFVKMEPLAKPALEAYKN
                     GELNFIPERFGKIYTHWLENIKDWCISRQLWWGHRLPVYYCQDCDEIMVGREKPEKCS
                     KCAATNICQDTDTLDTWFSSALWPFSTLGWPKDTEDLQYFFPTDVLVTDYDIIFLWVI
                     RMVFSSLEQMGQLPFKDVFLTGLVRDSQGRKMSKSLGNGIDPLEVIEVYGADALRFTL
                     VTGNTPGNDMRFHMERVEASRNFANKLWNATRFVLMNLDESVAEQEFRMEDLEEEDKW
                     ILSRLNRIIGEVTNNLDKYEIGLAAQRVHDFIWDDYCDWYIEMVKSRLYGDNKESKEL
                     AQQVLLYVLKDINKLLHPFMPFITEEIWQHLPGNEKDLIETTWPTTRKDLDFPQAEER
                     IQYVKESIKAIRNARAEMNIAPSRKATIIFVSREKDIRDLIERGRSHYINLASAEDIK
                     VQASKEGLGDDNVAVVLNKAEAFLPLKDLIDYEKEVERLEREKEKLEEELRRVRGKLA
                     NEGFVSKAPEKIVQEERDKLAKYENMMDKVLERLENIEKYRKI"
BASE COUNT          644 a          256 c          412 g          430 t
ORIGIN      
        1 caggtaaagg agcatgaaaa tgcagtgggc cactgtgaaa gatgtagaac agtaatagag
       61 cctataatat caaaacaatg gtttgttaag atggagcctc tggccaaacc agccctggaa
      121 gcatataaaa atggtgagtt aaactttata ccagaaaggt ttggaaaaat atacacccac
      181 tggctagaaa atataaagga ttggtgtatc tctagacaat tatggtgggg acacaggcta
      241 ccagtatatt actgccaaga ctgtgatgaa ataatggtgg gaagagagaa gccagaaaaa
      301 tgtagtaaat gtgctgcaac taatatttgc caagatactg acaccttaga tacctggttt
      361 tcctctgcac tatggccctt ctctactctc ggttggccta aggatacaga ggatttgcaa
      421 tactttttcc caacagatgt tttggttaca gactatgaca taatcttctt atgggtaatt
      481 agaatggtat tttccagctt ggagcagatg ggtcagctac cctttaagga tgtcttttta
      541 acaggactgg taagggacag ccagggaaga aagatgagca aatcactagg aaacggaatt
      601 gacccactag aagttataga ggtttatgga gcagatgccc taagatttac cttggtaaca
      661 ggcaatactc ctggaaatga tatgagattc catatggaaa gggtagaggc cagcagaaac
      721 tttgccaata aattatggaa tgctaccagg tttgtcctaa tgaatctgga tgaaagtgta
      781 gcagaacaag aatttaggat ggaagattta gaagaagaag acaaatggat cctatctagg
      841 ttaaatagga tcatagggga agtaaccaat aatctggaca aatatgaaat aggcctggca
      901 gcccaaaggg tccatgactt tatttgggat gattactgcg attggtatat tgaaatggtc
      961 aaatctagat tatatgggga taataaagaa agtaaagagt tggcccaaca agttttactt
     1021 tacgtattaa aggatataaa taaactactt catcccttta tgccctttat aacagaagaa
     1081 atatggcagc atctgcctgg aaatgaaaaa gatctaattg aaaccacctg gccaacaact
     1141 agaaaagatc tggattttcc acaggcagaa gaaaggatcc aatatgttaa ggaatccatt
     1201 aaggccatta gaaatgcaag ggcagagatg aatatagcac cttccagaaa ggctactatt
     1261 atctttgtaa gtagagaaaa ggacataaga gatttgatag aaagaggcag gagccactat
     1321 ataaatctgg ccagtgcaga agatataaag gttcaggcca gcaaagaagg tttaggagat
     1381 gacaatgtgg ctgtagtttt aaacaaggca gaggccttcc tacctttaaa ggatttaata
     1441 gattatgaaa aggaagtaga aagactggaa agggagaaag aaaaactgga ggaagaatta
     1501 aggagagtaa gaggaaaact ggccaatgaa ggttttgtgt ccaaggcgcc agaaaaaatt
     1561 gtccaagaag aaagggataa gctggccaaa tatgaaaata tgatggataa agtcttagaa
     1621 agactggaaa atatagaaaa atatagaaaa atatagataa ggtgggttat tgatggaata
     1681 cagggaagct ctatcttata taaacgataa gaataaattt ggctccaggc taggtttaga
     1741 ct
//
LOCUS       DATGSI010000477         1244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_31068, whole genome shotgun sequence.
ACCESSION   DATGSI010000477 DATGSI010000000
VERSION     DATGSI010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31068"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..>1244)
                     /gene="lepB"
                     /locus_tag="VK031_07380"
     CDS             complement(69..>1244)
                     /gene="lepB"
                     /locus_tag="VK031_07380"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HLS53778.1"
                     /translation="LFAVVAASIIHTYFIQPFTIPTSSLEKTLLVGDYLFVSKLHYGA
                     RAPMTAVSLPMVHDTIPVVKSKSYLNKPQLPYVRIPGFQKIKRNDIVVFNWPVDTLVD
                     INNPAKGSRIKPIDKKSNYVKRAVGVPGDSLSIVDKQMIINGEALELPDRAKLQFSYV
                     VVSEKPLNLNYIAQRYDITDIFPLNREFTGFQAYMTDEAYERLKSYPDIKSITPITHE
                     KGKVDNSIFPHDSRYGWNMDNFGPIYIPEKGATVAITPETIPFYKSIIEIYEGREMGI
                     DNKIQVDYGNVLLNGKPITEYTFLQDYYWMMGDNRHNSQDARNWGYVPFNHVVGKPVM
                     VFMSINNRLQGLGNKIRTERLFTTVHGKGEPTSYLYYVIAGIVLLIVGGEIRKRRKK"
BASE COUNT          356 a          224 c          237 g          427 t
ORIGIN      
        1 taattgcagt gcacaaggga aatatgcagg atagactaaa accttatcca taatattaaa
       61 atatatactt atttctttct tcgctttcta atttctccac ctacaataag aagtacgata
      121 cctgcaataa catagtacaa ataggatgta ggctcacctt ttccgtgtac ggtagtaaat
      181 aggcgttcgg ttcgtatttt attgcctaaa ccttgtagac ggttgttaat gctcatgaac
      241 accataactg gtttccctac tacatgatta aaaggtacat agccccaatt cctagcatct
      301 tgtgagttat gtcggttatc acccatcatc cagtaatagt cttgtaggaa ggtgtactct
      361 gtaatcggct taccgtttag aagtacattg ccgtagtcta cttgtatctt attatcaatt
      421 cccatttctc taccttcata tatttcgatg atagatttat agaacggaat agtttctgga
      481 gtgatagcaa cggtagctcc tttttcagga atatatatcg gtccaaaatt atccatattc
      541 caaccatatc ggctgtcatg tgggaagatg ctattatcca ctttaccttt ctcgtgggta
      601 attggggtaa tgctttttat atctggatag ctttttagac gttcataagc ctcgtcggtc
      661 atataagctt gaaatccagt gaactctctg ttaagtggga atatatctgt gatatcatat
      721 cgctgggcta tataatttaa attcaatggt ttttcactaa ctactacata agaaaattgg
      781 agctttgctc gatctggtaa ttctagtgct tcaccgttta taatcatttg tttatctaca
      841 atagagaggg agtctccagg aacaccaacg gctcgcttta cgtagttaga ttttttgtca
      901 ataggtttaa ttcggcttcc cttggctgga ttatttatgt ctactaatgt gtctacaggc
      961 cagttgaata ctacgatatc gtttcgtttt attttctgaa aacctggaat tctaacgtaa
     1021 ggtagttgag gtttatttag gtaagatttg cttttaacta caggaatggt gtcatgaacc
     1081 attggaagac taacagcagt cataggtgca cgagctccat agtgtagttt acttacgaac
     1141 aagtaatctc ccacaagtaa tgttttttct aaagaagagg tgggaattgt aaagggctga
     1201 ataaaatagg tgtggataat acttgctgct actactgcaa atag
//
LOCUS       DATGSI010000478         1338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_130315, whole genome shotgun sequence.
ACCESSION   DATGSI010000478 DATGSI010000000
VERSION     DATGSI010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130315"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /gene="rpsD"
                     /locus_tag="VK031_07385"
     CDS             complement(<1..548)
                     /gene="rpsD"
                     /locus_tag="VK031_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HLS53779.1"
                     /translation="MARYTGPVCRLCRREGMKLYLKGDKCYTDKCPITRRNYAPGQHG
                     QSRKKVTEYGLQLREKQKVKRFYGVGESQMRKYFNMADRMSGITGENLLGLLESRLDN
                     VVYRLGFASSRAEARQLVRHGHFTVNGQRADIPSIITQVGDVIEVKSKSRKNEKFKSL
                     VENHNGNTASWLTVDLEQLKGT"
     gene            complement(567..965)
                     /gene="rpsK"
                     /locus_tag="VK031_07390"
     CDS             complement(567..965)
                     /gene="rpsK"
                     /locus_tag="VK031_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HLS53780.1"
                     /translation="MATRRGKASRVRRRERKNIERGQAHISSTFNNTMVTLTDMQGNV
                     ISWASAGQLGFKGSRKSTPFAAQQAAEEAAKKAMEHGLRSVEVYVKGPGSGREAAIRS
                     LQATGLDVNMIKDVTPIPHNGCRPPKRRRV"
     gene            complement(982..>1338)
                     /gene="rpsM"
                     /locus_tag="VK031_07395"
     CDS             complement(982..>1338)
                     /gene="rpsM"
                     /locus_tag="VK031_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HLS53781.1"
                     /translation="ARIAGVDLPRDKRVEVGLTYIFGIGRSSSLEILKNAGVDPDTRV
                     KDLTEAEVGKIRELINDYQVEGDLRREVAMNIKRLREINCYRGIRHRRGLPVRGQRTK
                     TNARTRKGPKRLITKK"
BASE COUNT          319 a          330 c          182 g          507 t
ORIGIN      
        1 atagtaccct ttaattgttc taagtctaca gttaaccaag aagcagtatt gccattatgg
       61 ttttcaacca atgacttgaa tttttcattt ttcctgctct tagatttaac ctctataaca
      121 tctcctacct gggtgattat agatggtata tctgcccttt ggccatttac agtaaagtgg
      181 ccatgtctta ctagctgtct tgcctctgct ctagaggatg caaatcccaa tctatatacc
      241 acattatcta atctggattc caatagtcca agtaggtttt cacctgttat cccactcatc
      301 ctatcagcca tattaaagta tttcctcatt tgggactcgc caaccccata aaatcttttt
      361 accttttgct tctctcttaa ctgtaaaccg tactctgtaa ctttcttcct agattgtccg
      421 tgttgacctg gagcataatt tcttctagtt attggacact tgtctgtata acatttatcc
      481 ccctttaggt ataacttcat accctctcta cggcacaatc tacaaactgg acctgtatat
      541 cttgccattt gtatgcacct cctgatttaa actcttctac gttttggtgg tctacatcca
      601 ttatgtggaa ttggtgttac gtctttaatc atatttacat ctaaacctgt tgcttgtaaa
      661 gatctaatag ctgcctctct acctgagcca ggccctttaa cataaacctc cacacttctt
      721 agaccatgtt ccattgcctt tttagctgct tcctcagctg cttgttgggc agcgaaagga
      781 gtagactttc tggaaccttt aaatcctaat tgaccagcac ttgcccatga tattacatta
      841 ccttgcatat cagttaatgt aaccatggta ttattaaatg tagaagaaat atgtgcctgg
      901 cctctttcta tatttttacg ttctcttctt cttacacgac ttgctttacc tcttctggtt
      961 gccattcaac accctccttt tctacttctt agtaattagc cttttaggac cttttctagt
     1021 tctagcattt gtctttgtcc tttggcctct tactggtagg ccccttctat gcctgattcc
     1081 tctataacaa ttaatctctc ttaatctctt aatattcatg gcgacttctc ttcgtagatc
     1141 tccctctact tgatagtcat ttattagctc tcttatttta cctacttctg cttctgtcaa
     1201 atcctttact cttgtatctg gatctacacc agcatttttc aatatttcta aggagctaga
     1261 tctaccaatt ccaaagatat atgttaaacc tacttcaact ctcttatctc ttggtaagtc
     1321 aacacctgca attcttgc
//
LOCUS       DATGSI010000479         1184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_314548, whole genome shotgun sequence.
ACCESSION   DATGSI010000479 DATGSI010000000
VERSION     DATGSI010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314548"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VK031_07400"
     CDS             complement(<1..955)
                     /locus_tag="VK031_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HLS53782.1"
                     /translation="MVSEILSIINIKKNISKNDSSVKRSLSEKTISAFLWLGTGSGLQ
                     SIMQLLVLAILARLIDPSDFGVLHVTLIVVGFARLMSQMGIGPAIVQKNNLSYNHIYS
                     GFTISLVFGVILALILCLFSNAIEAFFKMHGLGFILKIISTIFIIESFIVIPQSLLQR
                     DLKLKEYATINIISYLFGYGLVGIGLAALGYGVYSLVWALVFQSILKACIATYLVPRF
                     YILTFHYKSLKELLYFGGGVTTAQFANYLAAESDSIITGRYLGAEALGLYSRAYSIMV
                     KPVNLLGSAVDKALFPSMSTIQKDHQRLKSAYLKGIGSLAYI"
BASE COUNT          401 a          212 c          167 g          404 t
ORIGIN      
        1 atatatatgc aaggctaccg attcctttga ggtatgctga ttttaacctt tgatgatcct
       61 tttgtattgt ggacatcgaa ggaaataaag ctttatccac ggctgagcct aatagattca
      121 ccggctttac cattatagaa tatgctcgac tataaagccc aagtgcttct gctccaagat
      181 acctacctgt tattatactg tcactctctg ctgcaagata atttgcaaat tgagcagtag
      241 tcactccacc tccaaaatat aacaattctt ttaaactttt ataatggaaa gttagaatat
      301 aaaatctagg cactaaatat gttgctatac aagctttcag tatagattga aatactagtg
      361 cccaaactaa tgaatacaca ccataaccca gtgcagccag cccaatacca accagaccat
      421 atccaaataa atatgaaatt atgtttatag ttgcatattc ttttaacttt agatctctct
      481 gtaatagtga ttgaggtatc actatgaaac tttctattat gaaaatagtt gatattattt
      541 ttaatataaa ccctaaacca tgcattttaa aaaaggcctc aattgcattt gaaaataagc
      601 acagtattaa ggctaatatt acaccaaata ctaaggagat agtaaatcct gaatatatat
      661 gattatatga tagattattt ttttgcacaa ttgctggacc tattcccatt tgactcatta
      721 atcttgcaaa acctaccact attagagtta catgtagaac tccaaaatca cttggatcta
      781 ttagtctagc aagtatggct aatactaata gttgcattat ggattgtaga ccactaccag
      841 ttccaagcca tagaaatgca gagattgttt tttcagaaag tgatctttta actgatgaat
      901 cgttctttga aatatttttc ttgatattaa tgattgataa tatctctgat accattttaa
      961 aaaggacccc actcctttct ctatagttgg actcgtctac taattaagat tatatatcaa
     1021 ttctatcata tcttttttta ttcacgctac atctccccat tatttaagat ataatagaat
     1081 ataatttttc tgaagcaagt gctttagttc aactataatt ttctactcgt agaaaattat
     1141 ctgtgagtaa taagtggtca ggactagcag ggcaagtaca taaa
//
LOCUS       DATGSI010000480         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_176369, whole genome shotgun sequence.
ACCESSION   DATGSI010000480 DATGSI010000000
VERSION     DATGSI010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176369"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VK031_07405"
     CDS             <1..544
                     /locus_tag="VK031_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HLS53783.1"
                     /translation="SDVDTIERMEIIRDLRLGKYDVLVGINLLREGLDLPEVSLVAIL
                     DADKEGFLRSETSLIQTTGRAARNVDGKVIMYADTITKSMERAIDETNRRRKLQDEYN
                     KKHNITPKTISKGVREIIEATLVAEDEIKYDEEFTNDEIESMIIGLEGAMLKAAEELE
                     FEKAAELRDKISDLRKMLKH"
     gene            558..>1529
                     /locus_tag="VK031_07410"
     CDS             558..>1529
                     /locus_tag="VK031_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HLS53784.1"
                     /translation="MTKDKIIIRGAKENNLKNVNIDLPRNKFIVFTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARQFLGQMDKPNVEYIEGLSPSISIDQKTTSRNPRSTVGT
                     VTEIYDYLRLLYARIGTPYCPKCGKVISSQTVDQMVDSIMELEERTRIQILAPVVRGK
                     KGAHVKVLENIKKEGFIRLMIDGELYDIADEIKLDKNKNHTIEVVVDRIIIREDVEGR
                     LTDSLETALRLADGLVVIDVIDGERLIFSQKLSCPDCNISMEELEPRMFSFNSPFGAC
                     PTCNGIGFYKTVDPDLVIPNKELSIAEGGIAPFSTSGENTYYYQVFKR"
BASE COUNT          558 a          204 c          361 g          406 t
ORIGIN      
        1 ttctgatgtg gatacgattg agaggatgga aataataaga gatttaaggt taggaaagta
       61 tgatgtatta gttggaatta atttactaag ggaagggctg gatctaccag aagtatccct
      121 ggtggccata ttagatgcag ataaggaagg atttttacgt tcagaaacat ccctaatcca
      181 gactacaggc agggctgcta gaaatgtgga tggtaaggtg attatgtatg ctgatactat
      241 tactaaatca atggaaagag ccatagatga gacaaataga agaagaaagc ttcaggatga
      301 atacaataaa aagcataaca taactcctaa aaccataagt aaaggagtta gggaaattat
      361 agaagcaact ttggttgctg aggatgaaat taaatacgat gaggaattta ccaatgatga
      421 aatagaatct atgataatag gcttggaagg agccatgtta aaggctgcgg aagaattaga
      481 atttgaaaag gccgcagaat tacgagataa aatatctgat ttaaggaaga tgttaaaaca
      541 ttgaggtgga taagtgaatg acaaaggata aaataatcat tagaggtgca aaagagaaca
      601 atctaaaaaa tgttaatata gatttaccaa ggaacaaatt tatagtgttt acagggttaa
      661 gtggctcagg taagtcttct ttagcatttg ataccatata tgcagaagga caaagacggt
      721 atgtagagtc gctttccagt tatgctagac aatttctggg ccagatggat aagccgaatg
      781 tggagtatat agaagggctg tcgccttcca tatcaataga tcaaaaaact accagtagaa
      841 atcctagatc tactgtaggg actgtaactg aaatatacga ttatttaagg cttttgtatg
      901 ccagaatagg tactccctat tgcccaaaat gtgggaaagt aatttctagc caaacagtag
      961 accagatggt agatagtatt atggagttag aggaaaggac cagaatccaa atcctagctc
     1021 cagtggtaag aggaaagaag ggagcccatg ttaaagtctt agaaaatata aagaaggaag
     1081 gcttcattcg attaatgata gatggagaat tatacgatat agcggatgaa ataaagctgg
     1141 ataagaataa aaaccatacc attgaggtag tggtagatag gattataata agggaagatg
     1201 tggaagggag attgacagat tctttagaaa ctgccttaag attggcagat gggctggtag
     1261 ttatagacgt tatagatgga gaaaggctta tttttagcca gaagctatcc tgtcccgatt
     1321 gtaatatttc tatggaagaa ttggaaccta gaatgttttc cttcaacagc ccctttggtg
     1381 cctgcccaac ctgcaatggg ataggttttt acaaaacagt agatcctgat ttggtaatcc
     1441 ctaataagga gttaagtatt gcagaaggag gtatagctcc ctttagcact tcaggagaaa
     1501 atacctacta ctatcaggtt tttaaaaga
//
LOCUS       DATGSI010000481         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_223583, whole genome shotgun sequence.
ACCESSION   DATGSI010000481 DATGSI010000000
VERSION     DATGSI010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223583"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VK031_07415"
     CDS             complement(<1..587)
                     /locus_tag="VK031_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53785.1"
                     /translation="MGNKKELLNYIKTVGKDRTWRQLANKFGYTSEDTVRKIVKRNLS
                     HYTETTTVTNISSDSTTSEESNETSYDFNKGEMKISHIWDHPPTPEEIIEHHNIDESK
                     WKLSNVWVKMKKGGYQTSALFSQKKSKDVTPDDIKRVLDNFTPPKLSLDSGSKINKKY
                     SSPCSAFIDITDFHLDKKTLDGDTIEDRVESYQKIV"
     gene            complement(760..999)
                     /locus_tag="VK031_07420"
     CDS             complement(760..999)
                     /locus_tag="VK031_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53786.1"
                     /translation="MASITFSYNITPNLKIDLYRRKAGSTASFNLEKADLTFDDSPYQ
                     IIVPSGHSYELKYITKDCKGNQIGDAKTILASKNS"
     gene            complement(999..>2086)
                     /locus_tag="VK031_07425"
     CDS             complement(999..>2086)
                     /locus_tag="VK031_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53787.1"
                     /translation="TANIHIQWDLNTQVDNVKVEYKKKAANTWTVPTSPANPTSNIQY
                     PLTVEMDVVYQVKLLTKMKTSCSSCEECDPYPVVYEFYVDSNEANNTVNCPPGYTLSD
                     DETVCFKVDTMPAQQESGGTTVTACHYTALEYANAGSWLFKKDGYNQDGTYKSGKNPY
                     HLTQNGLWRNGNLEKDRENKASIWACSSQTYSGDLGFSRSFHVPQNKTYYVALAGDNK
                     VKLEINGTVIIDQDPNALNAQSEFNQGGFADRTTFCNFFIYPVELSSGQNTLSILAHN
                     AGSIGMLSCEIYDATEAQIKSWSSHSQVENSIIFSTAKPSDGGNVGDNSNFDIGNWYC
                     ADPNYTLVYDEQSSSYSCQKIDRTNPI"
BASE COUNT          612 a          366 c          328 g          780 t
ORIGIN      
        1 actatttttt gataactttc tactctatcc tctattgtat caccgtcaag tgttttttta
       61 tccaagtgaa aatctgtaat atctataaat gcactacaag gagaggagta tttcttattt
      121 attttgctgc ctgagtccaa acttaactta ggtggtgtaa aattatcaag cactctcttg
      181 atatcatccg gggttacatc ttttgatttc ttttgtgaaa ataaagcaga tgtttgataa
      241 cctccttttt tcatcttaac ccatacatta gatagtttcc acttactttc gtctatatta
      301 tgatgttcta taatttcctc tggtgttgga ggatgatccc aaatatgaga aattttcatt
      361 tctcctttat tgaaatcata agaagtttca ttgctttcct ctgaagtagt tgaatcagaa
      421 gatatatttg ttacagttgt tgtttctgta tagtgagata agtttctttt aactattttt
      481 cttactgtat cttcacttgt gtaaccaaac ttattagcta gttgtcgcca agtcctatct
      541 ttgccgactg ttttaatgta attaagtaac tcttttttat tacccattaa atattataat
      601 tgattaatct gtaaagttag ggtatttcat tttaaaaacc aaatattttg gctttaacct
      661 tttgttaact atatacaaaa aataaagcct aaacctttgc aggagtaggc tttgagtggg
      721 aaaaccaact aaaacccact ttaatacttt aaattatttt taactgtttt ttgatgctaa
      781 tattgttttt gcatctccta tttgattccc tttgcagtct tttgttatgt attttaattc
      841 ataagaatga ccagatggga ctattatttg ataaggggaa tcatcaaatg ttaagtctgc
      901 tttctccagg ttaaatgaag cagtggaacc tgcttttctt ctgtataggt ctatttttaa
      961 gttaggggtt atattatatg aaaaagtaat actagccatt atattggatt tgttctatct
     1021 attttttgac aagaataaga agagctttgt tcatcataaa ccagagtata attaggatca
     1081 gcacaatacc agtttcctat atcaaagtta ctattgtctc ccacattccc cccatcagat
     1141 ggtttggctg tgctaaatat aatactattc tctacttgag agtgagatga ccaagattta
     1201 atttgtgcct ctgttgcatc ataaatttca caagataaca ttccaatact tcctgcatta
     1261 tgcgctaata tagataatgt attttgtcca gatgataatt ccacaggata tataaaaaag
     1321 ttacaaaaag tagttctatc tgcaaaacca ccttgattaa actcggattg ggcatttaag
     1381 gcattagggt cttggtctat tataactgta ccatttattt ctagcttaac tttattatct
     1441 cctgcaagag cgacataata tgttttattc tgtggaacat gaaaagaacg ggaaaatcct
     1501 agatcccctg aataagtctg actgctacac gcccaaatag aggctttgtt ctctctatcc
     1561 ttttcaaggt ttccatttct ccaaagccca ttttgtgtta aatggtaggg gtttttaccc
     1621 gatttatagg taccatcttg gttatatcca tctttcttaa ataaccatga tcctgcatta
     1681 gcatactcta aagcagtgta atggcaggct gttactgtgg tacctccact ctcctgttgt
     1741 gcaggcattg tatctacttt aaagcaaact gtttcatcat cagacaatgt gtaccctgga
     1801 gggcagttga ctgtattatt tgcctcatta gaatctacat aaaactcata cactacaggg
     1861 taaggatcac attcctcaca agagctacag gaagtcttca ttttagttaa aagcttaacc
     1921 tgataaacaa cgtccatctc tactgttaag ggatattgta tattagaagt tggattagca
     1981 ggagaagttg gtactgtcca agtattagct gccttcttct tatattcaac ttttacatta
     2041 tctacctgag tatttaaatc ccactgtata tgtatattag cagttg
//
LOCUS       DATGSI010000482         1743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_223594, whole genome shotgun sequence.
ACCESSION   DATGSI010000482 DATGSI010000000
VERSION     DATGSI010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223594"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VK031_07430"
     CDS             complement(<1..316)
                     /locus_tag="VK031_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53788.1"
                     /translation="MSIQDLINAASPGSIINIQPGLYKEQITIDKPLTLQGPSQSEPS
                     ATLDASDFESGPNIHILANNVVIRNLFIQDGLSHGILIGDENYPNLTGNMISDNHITG
                     HGN"
     gene            617..>1743
                     /locus_tag="VK031_07435"
     CDS             617..>1743
                     /locus_tag="VK031_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HLS53789.1"
                     /translation="MDLKLRRGIFVFFILLFIFIFLMFYSEFKNMDRLRKEYPNIEEE
                     VYRYRKDILKVWAIRLVLQFLLPLLFLTTGFSQKLSLWASHKRGLILSGFLYGLAFFV
                     ILFFLNLPLKYYSSFYLPHKYGISNQTFLRWIELNIKGLLVNNLIFSLFLWIPYYIAF
                     KSPKTWWLQIGLLIIPIIVFMVFITPMIIDPIFNKYTSIENEDLEYKISLLLDKAGIS
                     DAKVYKVDKSRDTKTMNAYMTGISKSKRIVLWDTTINNLTEEELLCIMAHEIGHYVQG
                     HIWKNIVVASCGTLLIMYLIYVTSNWILEYSYGTFGFRNIYNLASLPLFILVLNFYTF
                     LGNPIMNSISRYMEREADAYEIILTEDRESAITAMEKLYVE"
BASE COUNT          580 a          233 c          310 g          620 t
ORIGIN      
        1 cgtttccatg accagtaata tgattatcag atatcatatt accagttagg ttagggtagt
       61 tttcatcccc tattaatata ccgtgtgaga gaccgtcttg aatgaaaaga tttcttataa
      121 ctacattgtt agccaatatg tgaatgtttg ggccagattc aaaatcgctg gcatcaagtg
      181 tggcggaggg ttctgattgt gatggtccct gcagggtaag aggtttgtct attgttattt
      241 gctctttata taaacctggc tgtatattaa ttatacttcc aggactagct gcatttatta
      301 aatcttggat actcatatac atcactccaa tgttggaaaa aattatattt atatctattc
      361 aaaatattat attcacctat tttagatatg gtgaatagga aaagaaattg tagttttaac
      421 gagatgttta aatacatgaa atacttttca attaatatga ggtgggaaac tttatgatcc
      481 tctataaatt ggattaaatt aggctattag gttattttaa atataaggct agctaattgc
      541 atatgatata ataatcctat aaagtaatga tacttttgat ttataccatt agccaaaaac
      601 atgggaggtg gaggatttgg atttaaaatt aagaagagga atttttgtat tttttatact
      661 tttatttata tttatcttcc ttatgtttta cagcgaattt aaaaatatgg atagattaag
      721 gaaagaatat cctaatatag aggaagaagt atacagatat aggaaagata ttttaaaagt
      781 atgggccatc agattggttt tacagttttt acttcccttg cttttcttaa caacaggatt
      841 ttcccaaaaa ttaagcctgt gggccagcca taaaaggggc ctaattctgt caggttttct
      901 gtatggttta gcattttttg taatcttatt ttttctaaat ttaccactta aatattattc
      961 gtccttttat ttgccccata agtatgggat ttctaaccag acctttcttc ggtggataga
     1021 gttaaatata aaagggttgc tagtaaacaa tttaatattt tccttatttt tatggatacc
     1081 ttactatatt gcctttaaaa gtccaaaaac ttggtggtta cagataggat tgttaataat
     1141 tcctattata gtatttatgg tgtttataac acctatgata atagatccca tatttaataa
     1201 gtacacatca attgaaaatg aagacttaga atataaaata tctctactac tagataaggc
     1261 aggcatatcg gatgctaagg tatataaggt ggataaaagt agggacacca agactatgaa
     1321 tgcttatatg acaggaatct caaagtctaa acggatagtt ttgtgggaca ctacaattaa
     1381 taatttaaca gaagaggagc tactatgtat aatggcccat gagataggtc attatgttca
     1441 gggacatatt tggaagaata tagtagtagc cagttgtgga accttactta taatgtattt
     1501 aatctatgtt acatccaatt ggatattaga atattcatat ggtaccttcg gctttagaaa
     1561 tatttataat ttagcctcgt tgccactttt tattctggta ctaaactttt atactttttt
     1621 aggaaaccct ataatgaatt ccatatcaag atatatggaa agggaagcgg atgcttacga
     1681 aataatctta actgaggaca gggaatcagc tataactgcc atggaaaaat tatatgtgga
     1741 gag
//
LOCUS       DATGSI010000483         1067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_505480, whole genome shotgun sequence.
ACCESSION   DATGSI010000483 DATGSI010000000
VERSION     DATGSI010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505480"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /gene="thrB"
                     /locus_tag="VK031_07440"
     CDS             complement(<1..748)
                     /gene="thrB"
                     /locus_tag="VK031_07440"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HLS53790.1"
                     /translation="MIKVRVPATTANLGPGFDTLGLALNLYNEFTFTEIGRGFETIGF
                     KKAYKNSNNLVYSSMLTTFEKIGHRPKGIRIEANINIPISRGLGSSASCILAGVIGAN
                     ELVGGPLDMQEILELATEIEGHPDNVAPALFGGLVISLREQGKIIYNKMDIHAGLKFV
                     ALVPDFTLSTEEARSVLPEEISYQDAINNISRVSLLLSALANGKFGLLKTALKDRLHQ
                     PYRGCLIKDFDKVMAKVEELNGLGAYLSGAG"
     gene            complement(741..>1067)
                     /locus_tag="VK031_07445"
     CDS             complement(741..>1067)
                     /locus_tag="VK031_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HLS53791.1"
                     /translation="YLIDPHTAVAYGVYKKYLRKSQDKTKTLVASTASPFKFGKKVAS
                     SIGLDISDKDEFEILDMMSRIWKVELPESIKTLREKEVLHETVALKEKMKESVETFLR
                     VGDQND"
BASE COUNT          284 a          242 c          175 g          366 t
ORIGIN      
        1 gccctgcccc acttaagtac gctcccaggc cattcagctc ttccaccttt gccataacct
       61 tatcaaaatc tttaatgagg caacctctat aaggttggtg tagcctatcc tttaaggcag
      121 tctttaataa gccaaacttt ccatttgcca gagctgataa aagtaaggat acccggctta
      181 tattgtttat ggcatcctga taacttattt cttctggcag tactgatcta gcttcttccg
      241 tagataaggt aaagtctgga accagggcaa caaattttag gccagcatgt atgtccattt
      301 tattataaat tattttaccc tgctctctta aggatataac aagaccgcca aataaggctg
      361 gagccacatt atcaggatga ccttctattt ctgttgccag ttctaaaatc tcttgcatat
      421 ctaaagggcc tcctaccagt tcattggctc ctattactcc agccagaata caagaggcac
      481 tgctacctaa gcctctggaa ataggtatat ttatattagc ctctatcctg ataccctttg
      541 gcctatgtcc aattttctca aaggttgtta gcatggatga atagactaaa ttattactat
      601 ttttgtatgc ttttttaaag cctattgttt caaatcccct acctatttct gtaaatgtga
      661 attcattata taagtttagg gccaggccta aggtgtcaaa tcctggccct agattggcag
      721 tagttgctgg tacccttacc ttaatcattt tgatcaccta cccttaagaa ggtctccaca
      781 ctttccttca tcttttcctt taaagctaca gtttcatgta gaacttcttt ttccctaagt
      841 gtttttatgc tttctggtag ctctactttc caaatcctag acatcatatc taagatttca
      901 aattcatcct tatcacttat atccaaacct atagaagagg ccactttttt tccgaactta
      961 aagggactgg ctgtggaagc tacaagggtt ttagttttgt cctggctttt ccttagatac
     1021 tttttatata ccccgtaagc aactgcagta tgagggtcta taaggta
//
LOCUS       DATGSI010000484         1687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_223773, whole genome shotgun sequence.
ACCESSION   DATGSI010000484 DATGSI010000000
VERSION     DATGSI010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223773"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VK031_07450"
     CDS             complement(<1..458)
                     /locus_tag="VK031_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HLS53792.1"
                     /translation="MDDTSRYRDLFFEETDEYLQVLNDSLLELERNPEDNLILDEIFR
                     AAHTLKGMAGTMGYNTMAELTHHMENVFELFRQGSVKVTSELITVIFKCVDKLAEIVE
                     DLRMENYFEYNIEDLIMELEQIALEDKKGKELAKEDGSKEDLTLENFLNNL"
     gene            complement(485..1258)
                     /locus_tag="VK031_07455"
     CDS             complement(485..1258)
                     /locus_tag="VK031_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HLS53793.1"
                     /translation="MKLDFDYFYTWAMDKLNLDLQSYKQRQLQRRISTVMKTAGAQNL
                     YQYSKLIEEDEEVKKAFLDYITINVSEFFRNKDIFKEFESVVVNRLSKKFNSLKIWSA
                     ACSIGAEPYSLAIILDKSGIRPRQKILATDIDDGILERAREAVYKEHEVRNVDKKTLD
                     EYFRQKDKLYHLEPRIKNMVQFKKHDLILDDYEQGFHAIICRNVVIYFKKETKDMIYK
                     KIADSLVTGGVLFTGATESIYNPKSFGLKKISTFIYEKV"
     gene            complement(1258..>1687)
                     /locus_tag="VK031_07460"
     CDS             complement(1258..>1687)
                     /locus_tag="VK031_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HLS53794.1"
                     /translation="FAERLDREAKIKVVEAKDNMIIERNTVYLAPGDYHMLIEGNRIR
                     LNSKEKIYGVRPAVDYLFESAARKYKERLLGIILTGMGRDGAKGMKAIKEFGGCNIAQ
                     SEESCVVYGMPRHAVESGVVDAVLGLEEISNSLNKLLKVK"
BASE COUNT          459 a          377 c          219 g          632 t
ORIGIN      
        1 agattgttta agaagttttc aagggttaag tcttccttag aaccatcctc ttttgctagt
       61 tcttttcctt ttttatcttc tagggcaatt tgttctaatt ccattataag atcttcaata
      121 ttatattcaa agtagttttc cattcttaaa tcttccacaa tttcggccag cttgtctaca
      181 catttgaaaa taactgtaat aagctctgaa gtaaccttaa cactaccttg ccggaatagt
      241 tcaaatacat tttccatatg gtgggtaagt tccgccatag tattgtatcc catggtgcct
      301 gccattcctt tcaaggtatg agcagcccta aatatttcat ccaggatcag gttatcttca
      361 gggtttcttt ccaactccag taaagaatca tttaatactt gaagatattc atctgtttct
      421 tcaaagaata agtccctata tctgcttgta tcgtccaatt aatccaacct cccatttatg
      481 taacttacac cttttcatag ataaaggttg atatcttctt caatccaaag gacttaggat
      541 tgtaaatact ttctgttgcc ccagtaaata gcactccccc tgtaactaaa gagtctgcta
      601 tttttttgta tatcatgtcc ttagtttctt ttttaaagta tattaccaca tttctacaga
      661 ttatggcatg gaaaccttgt tcatagtcat ctaggattag atcatgtttt ttgaactgga
      721 ccatattctt aattctaggc tctagatggt atagcttatc tttttgccta aagtattcat
      781 ccaaggtttt tttatccaca tttctaacct catgttcctt atatacagcc tccctagccc
      841 tttctaaaat cccatcatct atatctgtgg ccaaaatttt ttgtctcggc ctaatcccgc
      901 ttttatctag tataatggcc aaggaataag gttctgcccc aatggagcag gcagcactcc
      961 agattttaag tgagttgaat ttctttgaaa gtctatttac cactacagat tcaaattcct
     1021 taaatatatc cttattcctg aaaaactcag atacattaat agttatataa tccaaaaatg
     1081 cctttttaac ttcttcatcc tcttctatta atttggaata ctggtacaaa ttttgagctc
     1141 ctgcagtttt catcactgta ctaatccttc tttgtaactg tctttgttta taggattgta
     1201 gatccaggtt taatttgtcc atggcccaag tataaaagta atcaaaatct aatttcatta
     1261 tttcaccttt aataatttat ttaaactatt ggagatctcc tccagtccta acactgcatc
     1321 aactactcca ctttctactg catgtctagg catgccatat actacacaac tttcctcact
     1381 ttgggcaata ttacaacctc caaactcttt tatcgccttc attcccttgg ctccatctcg
     1441 ccccatgcca gtgagtatta tgcctagtag cctttccttg tactttctgg cagcagattc
     1501 aaataaataa tctacagctg gcctgactcc atagatcttt tcctttgaat ttagccttat
     1561 tctatttcct tcaatcaaca tatgataatc tcctggtgct agataaactg tattcctttc
     1621 aattatcata ttgtctttag cctctactac cttgatttta gcttccctat ccaacctttc
     1681 tgcaaag
//
LOCUS       DATGSI010000485         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_505531, whole genome shotgun sequence.
ACCESSION   DATGSI010000485 DATGSI010000000
VERSION     DATGSI010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505531"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /gene="glyA"
                     /locus_tag="VK031_07465"
     CDS             complement(<1..841)
                     /gene="glyA"
                     /locus_tag="VK031_07465"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009344705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HLS53795.1"
                     /translation="MKFTNMEKHDPIIKDLIESEIKRQNTHLELIASENFVSEQVMEA
                     MGSQLTNKYAEGYPGRRYYGGCDIVDKIEDVAIDRLKQLFGAEHANVQPHSGSNANLA
                     VYFAFLEPGDKVLGMNLSQGGHLTHGSPVNISGTYYNFIDYGVDKETEKIDYEQVRKI
                     ALKEKPKMIVAGASAYPREIDFKRFREIADEVGAYLMVDMAHIAGLIAADLHQNPVPY
                     SDFVTTTTHKTLRGPRGGAILCKKRYAKEIDKAIFPGIQGGPLMHIIAAKAVSFGEAL
                     EESF"
     gene            988..1776
                     /locus_tag="VK031_07470"
     CDS             988..1776
                     /locus_tag="VK031_07470"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HLS53796.1"
                     /translation="MDNFSIDSREEVRKFIKLVLSIGTLLLKSGAEIYRVEDTMNRVC
                     NSRSNISNADSFVTNTGIFITLEYDGEVFTRIKRVRDISIDLNKINLLNDFSRKFVNQ
                     NISIDEGLETIKQIQNTKSYSIPLKIFAGGILSGFFSQMYGGNMYDFIASFIAALLTL
                     FILDRLSKYKLTFFIDSFIGAFLASFFSCLLFAFNIGHSLDMIIIGAIMCLVPGVSIT
                     NSIRDTMSGDSLAGLSKGMEAIFSALAIAFGVGIVLNLYMKGVI"
     gene            1777..2214
                     /locus_tag="VK031_07475"
     CDS             1777..2214
                     /locus_tag="VK031_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HLS53797.1"
                     /translation="MELILQFIFSFFATAGFSIFFNAPFKSILPTGIAGALSWTLYYI
                     INQNYNHKIIGVFLGAFLVGLFGEFLAIKLKKPATVFITPGIVPLVPGAGMYYTMLNL
                     VQNNFSKAASFGAETFFLAASIAIGVIASTIFSRSIRSFKGQG"
     gene            complement(2211..2477)
                     /locus_tag="VK031_07480"
     CDS             complement(2211..2477)
                     /locus_tag="VK031_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HLS53798.1"
                     /translation="MKDNSAIIRRLRRIEGQIKGIQKMVEEEKFCGDILVQIAAARSA
                     LNNAGGLILENYMKNCLLQYIEGDSTTEDLDKLVDVLLKYSKQN"
BASE COUNT          737 a          492 c          414 g          961 t
ORIGIN      
        1 taaaactctc ctctagggct tcaccgaagg atacagcctt agctgcaatt atatgcatta
       61 agggccctcc ttgaatacct gggaaaatag ccttatcaat ttcttttgca tacctctttt
      121 tacataggat tgctccaccc cttggccctc ttaaagtctt atgagtagtg gttgttacaa
      181 agtcagaata aggtactgga ttctgatgta aatcagcagc tattaagcca gctatatggg
      241 ccatatctac cattagatag gcacccactt catctgcaat ttccctgaat cttttaaaat
      301 ctatttccct aggataagca ctggctccgg ctactatcat ttttggtttc tcttttaggg
      361 ctatctttct gacttgctca taatctattt tttctgtttc cttgtctact ccgtagtcaa
      421 taaagttata ataggttcct gatatgttta caggactgcc atgggttaga tgacctcctt
      481 gggaaagatt catacctaat accttatctc ccggctctag aaaggcaaaa tagactgcca
      541 gattggcatt tgagcctgaa tgaggttgaa catttgcatg ctctgctcca aagagctgtt
      601 tcaatctatc tattgccaca tcttcgattt tatctacaat atcacagcct ccatagtatc
      661 ttctgcctgg atatccttct gcatacttgt tggtcaactg gcttcccata gcctccatta
      721 cttgttcaga tacaaagttt tccgaggcaa ttaattctaa atgtgtgttt tgtctcttta
      781 tttcactttc aattaaatct tttataatcg gatcatgttt ttccatattt gtaaatttca
      841 taaaaataat ctcccctctt agcttatctt ttgttgatta tatttaccca atattgtaat
      901 ataattctat actggcaatt agtaattatt tcaataatgc ctattatacc atatttaaaa
      961 gacttttttc ttgaagaggt gcaatttatg gataattttt caattgatag cagagaagaa
     1021 gttagaaaat ttataaaatt agtattatct attgggactc ttttattaaa aagtggtgct
     1081 gaaatttatc gagtagagga cactatgaat agggtatgta attctagaag taatatttcc
     1141 aatgctgatt cctttgttac taatacagga atttttatta cattagagta tgatggtgaa
     1201 gtatttaccc ggattaaaag ggttagagat atatctattg acctaaataa aattaattta
     1261 ttaaatgatt tttccagaaa atttgttaac caaaatattt ccattgatga aggtctggaa
     1321 acaatcaagc agatacaaaa tacaaaaagt tactctatcc ccttgaaaat atttgcagga
     1381 gggattcttt ctggattttt cagccaaatg tatgggggca acatgtatga ttttatagcc
     1441 agctttatag ctgccttact taccttattt atccttgata gattaagtaa atataaatta
     1501 acttttttta tagatagctt cataggagca tttttagctt cctttttttc ctgtctttta
     1561 tttgccttta atataggtca ttctctagat atgattatta taggagccat catgtgcctg
     1621 gttcctggag tgtccataac aaattccata agggatacaa tgtctggtga ttctctggca
     1681 ggcttgtcta agggtatgga ggctattttc tccgccctgg ccattgcctt tggagttggt
     1741 attgtactca atctatatat gaaaggagta atataggtgg aacttatttt acaatttatt
     1801 ttttcctttt ttgccacggc aggtttttcc atctttttta atgcaccctt taaatccata
     1861 cttcctactg gcattgccgg tgccctgtct tggaccctat actatataat taaccaaaat
     1921 tataaccaca aaataattgg agttttttta ggtgcctttt tagttggatt atttggtgaa
     1981 tttttagcca tcaaattaaa aaagccagct acagttttta tcactcctgg aatagttcca
     2041 ttagtacctg gcgctggcat gtattacacc atgttgaatt tggtccaaaa taatttttcc
     2101 aaggcagcta gctttggtgc tgaaaccttc tttctagctg cttccatagc aataggtgtt
     2161 attgcatcta caatattttc ccgttctatt agaagcttta aagggcaggg ttaattttgc
     2221 ttagaatact ttaataaaac gtcaaccaat ttatccaagt cctctgttgt cgagtcgcct
     2281 tctatatatt ggagtaggca atttttcata taattttcca aaattaaccc acctgcatta
     2341 tttaaggcag atctggcagc tgctatttgt accagtatat ctccacaaaa tttctcttcc
     2401 tctaccattt tctgaatacc cttgatctgt ccttctattc tccttagtct tctaattatt
     2461 gccgaattat ccttcaaata ccaatccctc ctacaagatt cttacaattc ttattgatga
     2521 atctgctttc ttagaaacct ttttatctat aactctaaca atgaggaagg aaatggccat
     2581 ggttataaaa ccagtaaaga agct
//
LOCUS       DATGSI010000486         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_31850, whole genome shotgun sequence.
ACCESSION   DATGSI010000486 DATGSI010000000
VERSION     DATGSI010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31850"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VK031_07485"
     CDS             <1..312
                     /locus_tag="VK031_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004822657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HLS53799.1"
                     /translation="IFDVRFLPNPYYIDELKKVSGINKETKEYVFKWPQTNIFVSKIT
                     EMLEFLIPYYVEEGKTQLVIGIGCTGGFHRSVAIANKIGEILKENGHRTIINHRDLKE
                     D"
     gene            324..1331
                     /locus_tag="VK031_07490"
     CDS             324..1331
                     /locus_tag="VK031_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HLS53800.1"
                     /translation="MLDRSKTIELKEYQPKVVVIGGGTGLSILLRGLKQFTTNITAIV
                     NVADDGGGSGVLREDLGMLPPGDIRACMLALANTEPTMEKLLNYRFDSGLLKGQSFGN
                     LFLAAMNGIYGSFEMGIKEASNVLALTGQVVPVTLEKVCLYAELENGIYVKGESNIPI
                     VSKEQNSRIKRVFIDPAISNPLEEGIEAIQEADLIVLGPGSLYTSIIPNLLVNNIVDT
                     IYKAKAPKIYIVNVMTQPGETDGYGVMDHIEALLSHSREDLLDYVIVNTELIPEDIVL
                     ERYLTDGSSPVLLKNKEMKIISNLGIKLIKGKLIDIKKDYIRHDNIKLSKMLINMAEE
                     R"
     gene            complement(1334..1840)
                     /locus_tag="VK031_07495"
     CDS             complement(1334..1840)
                     /locus_tag="VK031_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53801.1"
                     /translation="MINEKFIINLQGKKFVTYEGLLDLAHKKGLLSIQVDLLQIPNQD
                     NDMTAICRAVATTEDSKFIDIGDANPRSVNSALVPHIIRMASTRAKARALRDLTNVGM
                     TAVEELSMVEEDSAPISQEDPPTRRQMETIRGLAEELNIPIEYNKLTKATAGNLIGKL
                     LDEKKGKS"
     gene            2057..3010
                     /gene="whiA"
                     /locus_tag="VK031_07500"
     CDS             2057..3010
                     /gene="whiA"
                     /locus_tag="VK031_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HLS53802.1"
                     /translation="MSFSASTKNEICRIPIEEFCCALAELAAFVRMSGTIKILGGKQI
                     NLNFTTENAAIARRIFTILKELYDTEVQVVVRRNRQLKKNNNYLIMVNNSSISKRILK
                     DVGFIDKSNQTLLNSFYKVDGNLIGNRCCKRSYIRASFLGGGSISNPEKSYHLELVTS
                     NQEHGKDLSKIINSFGLKSKIVLRKDNHVIYIKEGEQIVDFLNIVGAHQALLKFEDIR
                     VLKDVRNNINRLVNCETANLGKTIDASMRQIEDIKLVQRRLGLNKLPKNLREVAELRL
                     SHREASLKEIGKMLNPPVSKSGVNHRFRKITQIARELGGDK"
     gene            3010..3276
                     /locus_tag="VK031_07505"
     CDS             3010..3276
                     /locus_tag="VK031_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HLS53803.1"
                     /translation="MEKVTLTITNEDGLHARPAAIFVQEANKFESDIELIFDGNKVNG
                     KSIIGVMSLGAYTGDEITIIAKGIDEEEAIKTLEELITDGLKDI"
BASE COUNT         1270 a          473 c          703 g         1011 t
ORIGIN      
        1 atatttgatg taagattttt accaaacccc tattatatag atgagcttaa aaaagtgtct
       61 ggaataaata aggaaactaa ggagtatgta tttaaatggc cacagaccaa tatatttgta
      121 tctaaaatta ctgaaatgtt agagttttta attccctact atgtagagga gggcaagact
      181 caattggtaa taggtatagg ctgtacagga ggtttccaca gatctgtggc aattgccaat
      241 aaaataggag aaattttaaa agaaaatggt catagaacaa taataaacca tagagaccta
      301 aaagaggatt aaggggaata tatatgttgg ataggagcaa aaccattgaa ttaaaagaat
      361 atcaaccaaa ggttgtggta ataggtggag gtacaggcct atcaatccta ctaagggggc
      421 taaaacaatt taccactaat ataactgcca tagtaaatgt ggcagacgac gggggaggtt
      481 caggggtttt aagggaagac ctgggcatgt tgcctcctgg agatattagg gcttgtatgt
      541 tggctctagc caatacggag ccaaccatgg aaaaattatt aaattacagg tttgatagcg
      601 ggctcttaaa gggacagagc tttggaaatc tttttttggc agccatgaat gggatctatg
      661 gaagctttga aatgggaata aaagaggcca gcaatgtttt ggctttaaca gggcaagtcg
      721 ttccagtaac tttagaaaag gtttgcttat atgcagaatt ggaaaatgga atatatgtaa
      781 agggtgagtc taatatccct attgtatcta aggagcaaaa tagcagaata aagagggtat
      841 ttattgatcc agccatttca aaccctctag aggaagggat agaagcaatc caggaggctg
      901 acctaatagt cctagggcca ggtagcttgt atacgtccat aataccgaac ctactggtaa
      961 acaatatagt agataccatt tataaggcca aggcccctaa gatatatata gtaaatgtga
     1021 tgactcaacc gggagaaact gatggctatg gtgttatgga tcatattgag gccttattaa
     1081 gccatagtag agaagattta ttagattacg ttatagtaaa tactgagtta atacctgaag
     1141 atatagtctt agaaagatat ttaacagatg gttcatcgcc tgtgctactt aaaaataaag
     1201 agatgaaaat aatcagtaat ttgggtataa aattaataaa agggaagcta atagatatta
     1261 aaaaggatta tatacgccat gacaatataa aattaagtaa aatgctaata aatatggcag
     1321 aagaaagata aatctaggac ttacctttct tctcatcaag tagttttcct attaaattac
     1381 cagctgtagc cttagttagt ttattatatt caatcggaat atttaattcc tctgctaagc
     1441 ccctaatagt ttccatttgt cttctagtag gtggatcttc ttgtgaaata ggagctgaat
     1501 cttcttcaac catagacaat tcttcaacag cagtcatacc tacattggta agatctctaa
     1561 gtgctctggc cttggctctt gtggatgcca tcctaataat atggggcacc aaagcagaat
     1621 ttactgacct aggattagca tctcctatgt ctataaactt tgaatcttca gtagtggcta
     1681 ctgctctgca tatggccgtc atatcattat cttgattagg tatctgtaat aggtctacct
     1741 ggattgataa tagccccttt ttatgggcaa gatctagcaa gccttcatag gttacaaatt
     1801 tctttccttg taaattgata ataaattttt catttatcat aagatcaccg taactatata
     1861 gtacccaagg agagatacta atatttaaaa ttaaaagaat ttttatttgg aaactttaac
     1921 aaattaaaga gaaagtatag tataattgat ataataataa ctaaggatcc tgttttaata
     1981 aatatattta ctttggcaat ttgaaagtct taatagttta tgtcaaagtc aggctaaaaa
     2041 ggccggaggt gataggatgt cattttctgc atccaccaaa aatgaaatat gtagaatccc
     2101 tatagaggaa ttttgttgtg ctttagctga attggcagct tttgtaagga tgagtggcac
     2161 aatcaagata ttagggggaa aacaaattaa cttgaatttt actacagaaa atgcagccat
     2221 tgcaaggaga atatttacta ttttaaaaga gctatatgat acagaggttc aagtagtagt
     2281 tagacgaaat aggcaattga agaagaacaa caattatctt ataatggtta ataatagctc
     2341 tatatctaaa agaatactaa aagatgtagg tttcatagat aaaagtaacc aaactttatt
     2401 aaattcattt tataaggttg atggaaatct tattggtaat agatgttgca aacgttccta
     2461 tataagggct tcttttctgg gagggggctc aattagtaat ccagagaaaa gttaccacct
     2521 agaattggta acaagcaacc aggaacatgg taaagaccta tctaaaatta taaattcttt
     2581 tggactaaaa tcgaaaatag ttcttagaaa ggataaccat gttatttata taaaagaggg
     2641 agagcaaata gtagattttt taaacatagt aggtgcccat caggcccttt taaaatttga
     2701 agatattagg gtattaaagg atgtaagaaa caatatcaat aggctggtaa attgtgaaac
     2761 agctaactta ggcaagacca tagatgcttc catgagacaa atagaagaca ttaaactggt
     2821 acaaagacga ttaggcttaa acaaattgcc taaaaatcta agagaggtag ctgagttaag
     2881 attaagccac agggaggcat cattaaaaga aatagggaag atgttaaatc caccagttag
     2941 caaatctgga gttaatcata gatttagaaa aattacacaa attgcaagag aattaggagg
     3001 agataagtaa tggaaaaagt aactttaaca attactaacg aagatggact acatgcaagg
     3061 cctgcagcta tttttgtgca ggaagcaaac aagtttgaaa gtgacataga gcttatattt
     3121 gatggaaata aggtcaatgg taaaagtata ataggggtaa tgtccctggg agcatacaca
     3181 ggggatgaga taacaattat tgctaaaggg attgacgagg aggaggcaat aaagaccctg
     3241 gaagaattga taactgacgg attgaaggat atataaaagg cagtggggaa atctttaaat
     3301 ctataatttt tgagaaggga agagctaaat caatttaagg atttaaacct aacttaaaat
     3361 aagtgtatga aggatattaa tatgtgatag cataaataaa ctttttatct tccctttaga
     3421 aggtctaaag attatagatt taccgcttat ttaatat
//
LOCUS       DATGSI010000487         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_130968, whole genome shotgun sequence.
ACCESSION   DATGSI010000487 DATGSI010000000
VERSION     DATGSI010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130968"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /gene="groEL"
                     /locus_tag="VK031_07510"
     CDS             <1..1122
                     /gene="groEL"
                     /locus_tag="VK031_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HLS53804.1"
                     /translation="TVEESRSIGTTLEVVEGMQFDRGYVSPYMVTDTEKMIAEYEDPY
                     ILITDKKISNIQEILPILEEIAQQNKPLIIIAEDIEGEAMATLVVNKLRGTFNCAAVK
                     APGFGDRRKEMLQDIAILTGGQVISEELGYELKDATIDMLGSAAKVKIDKEDTVIVDG
                     AGDQEEIENRVNQIRAQLEETDSEFDAEKLQERLAKLAGGVAVIQVGAATETELKERK
                     SRIEDALAATRAAVEEGIVPGGGTVLLDAIPEVAKLLDETEGDANTGVRIILKALEEP
                     VRQIASNAGLEGSVIVEKVKAEDSGVGFDALNEKYVNMIEAGIVDPTKVTRSALQNAA
                     SVAAMVLTTEAAVVDIEEEDPMAGMGAGAGGMGGMGGMM"
     gene            1305..1868
                     /locus_tag="VK031_07515"
     CDS             1305..1868
                     /locus_tag="VK031_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53805.1"
                     /translation="MSRNHTIYILLSHSGSIFSKAINMYTRGPYTHVSIALDEDLKEL
                     YSFGRLKPHNPIFGGFIKEDIVNGTYRRFPKTKCILYSLKINKQQYEKLKRELNRFKR
                     EADKYGYNFLGIIGAMFNHPIQRRYNYFCSQFVSEILHKSDIKILDKDPGLTAPMDFA
                     NCKELEFIYEGYLNLYNQERKEELLQL"
     gene            2044..>2562
                     /locus_tag="VK031_07520"
     CDS             2044..>2562
                     /locus_tag="VK031_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLS53806.1"
                     /translation="MEKEGLLIEKAKRGDELAFAQLIDLYKSYIFAIILNFIKDYEEV
                     ENVAQEVFLQVYTSLPGFENDNFKAWISRIATNKSIDSLRRKRSKFKEETMEETEILV
                     DSLGVYNDNNPESDFFKRKDMEEINKVLNSLPQIYVDVIRKFYFEEKSYEEISKEENI
                     SYKTVESRLYRGR"
BASE COUNT          987 a          333 c          544 g          698 t
ORIGIN      
        1 actgttgagg aatccagatc cataggtaca actctagaag ttgtagaggg tatgcaattt
       61 gatagaggct atgtgtctcc ttatatggtt acagacactg aaaagatgat agcagagtat
      121 gaggatccat atatccttat aactgacaaa aagatctcaa atattcaaga aatattacca
      181 atactagaag aaatagccca acaaaataag ccattgataa tcattgctga agatattgaa
      241 ggggaagcca tggctacact tgtagtaaac aagctaagag gaaccttcaa ttgtgcagca
      301 gttaaggcgc ctggctttgg agatagaaga aaagaaatgc tacaggatat agcaatcctt
      361 acaggcggtc aagtaatcag cgaagaatta ggatacgaac tgaaagatgc cacaatagat
      421 atgttaggaa gcgctgcaaa ggttaaaata gataaggaag atactgtaat agtagacgga
      481 gctggggatc aagaagaaat agaaaatagg gtaaatcaaa ttagagccca attagaggaa
      541 acagattcag agtttgacgc agaaaaacta caagaaagac tggctaaatt agcaggtgga
      601 gtagcagtta tccaggtagg tgctgcaact gaaacagagc tgaaagaaag aaaatcaaga
      661 atagaagatg ctctagcagc aacaagggct gcagtagaag aaggtatagt acctggtggt
      721 ggaacagtac tactagatgc tatcccagaa gtagctaaac tattggacga aactgaaggt
      781 gacgcaaaca ctggagtaag gattatatta aaagctttag aagagccagt aagacaaata
      841 gcaagcaatg caggattaga aggatctgta atagttgaaa aagtaaaggc agaagacagt
      901 ggtgtaggtt ttgatgctct aaatgaaaaa tatgtaaata tgatagaagc tggaatagta
      961 gacccaacta aggtaactag atcagcatta caaaatgcag ccagtgtagc agccatggtt
     1021 ctaactacag aggcagctgt agtagacatt gaagaagaag atccaatggc tggcatgggt
     1081 gcaggagccg gcggaatggg tggaatgggc ggtatgatgt aagccctaag ataaaagttt
     1141 aggcaacaca ctgcttatat aaagggtctg tcataggttt ataaacttat gacaggcttt
     1201 ttttatttta ataaatcctt ttgctgacag gtttaattta aattctattg aataaatata
     1261 caaaacaatg tattattaat atattattta aatgaggtgt aggcatgtca aggaatcata
     1321 caatttatat tttattatcc cacagtgggt ccatattttc caaggccata aatatgtata
     1381 ctagaggccc gtatactcat gtttccattg cactagatga agatttaaaa gaactctata
     1441 gttttggaag gctaaaacct cataatccta tatttggtgg ttttattaag gaagatatag
     1501 tgaatggaac ctataggagg tttccaaaaa ccaagtgtat actttattca ttaaagatta
     1561 ataagcaaca atatgaaaag ctgaaaaggg aattaaatag gtttaaaaga gaggcagata
     1621 aatatggcta taacttttta ggtataatag gagccatgtt taaccatcct atccaaagga
     1681 gatataatta tttttgttcc caatttgtat cagaaattct gcataaaagt gatataaaaa
     1741 tattagataa ggacccaggt ctaacagccc caatggattt tgctaactgc aaagaattag
     1801 aatttattta tgaagggtat ttaaacctgt ataaccagga aagaaaagaa gaattattgc
     1861 aattataaat tttatacaag aactagggat taccctagtt tttttatttg gaatatatat
     1921 agaaagtact tatataaaga ctacatatta aaacaattag gtataattga aataaatatg
     1981 aattttcttg cagtgattat gttttaattt acgttatatt taatgatagg aaggagggga
     2041 ggtatggaaa aggaaggact acttattgag aaagctaaaa gaggggatga attagctttt
     2101 gcccaattaa tagatctgta taaaagttat atatttgcca ttatattaaa ctttataaag
     2161 gattatgagg aagtagaaaa tgtggcccaa gaagtatttt tacaagtcta tacctccctt
     2221 cccggctttg aaaatgataa ctttaaggct tggattagcc gtatagcaac caataaatcc
     2281 atagattccc tgagaagaaa aagaagtaaa tttaaagaag agaccatgga agagacggaa
     2341 atcctagtag actcattagg tgtatataat gataataatc cagagtcaga cttttttaaa
     2401 cgaaaggata tggaggaaat aaataaagtt ttgaactcct tgccacaaat atatgtggat
     2461 gtaataagga agttttattt tgaagaaaaa agctatgagg aaattagtaa ggaagaaaat
     2521 atttcctata aaactgtaga atcaaggctt tatagaggga ga
//
LOCUS       DATGSI010000488         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_130970, whole genome shotgun sequence.
ACCESSION   DATGSI010000488 DATGSI010000000
VERSION     DATGSI010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130970"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VK031_07525"
     CDS             complement(<1..1114)
                     /locus_tag="VK031_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HLS53807.1"
                     /translation="MGKKMMTMDGNMAASYVSYAFTDVAAIYPITPSSDMAEHVDAWA
                     AEGRKNIFGQEVLVKELQSEAGAAGAVHGALQAGALTTTYTASQGLLLMIPNMYKIAG
                     ELLPGVFHVSARALASHALSIFGDHQDVMAARQTGFALLASGSVQEVMDLGGVAHLAA
                     IKGRVPFLHFFDGFRTSHEVQKIEVIEYEDLEKLLDKEALQEFRERALNPENPVTRGT
                     AQNPDIYFQAAEASNPFYDEIGEVTYDYMRKISELTGREYKPFVYYGHPEAERIIVAM
                     GSVTQTIEETIDYLVDRGEKVGLIKVHLYRPFVAEYFFDVLPETVKKIAVLDRTKEKG
                     AIAEPLHLDVNTIFYNRDEKPVIVGGRYGLGSKDTTP"
     gene            complement(1327..2259)
                     /gene="hprK"
                     /locus_tag="VK031_07530"
     CDS             complement(1327..2259)
                     /gene="hprK"
                     /locus_tag="VK031_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HLS53808.1"
                     /translation="MNSIDLLQIIEDLDIEIICKSSSIEDMEIHSSEISRPGLQIAGH
                     YDKFVPERLQIIGKAEWTYLSKLPSNERYEKLDKFLSFTIPALIVSRGLPVFPELIDL
                     AKKYDRTILRSDKSTSRIINETINTLNFYLAPETRVHGVFVEVYGVGVLITGKSGVGK
                     SETALDLVMRGHRLISDDIVIIKRLEDKLIGKSPEITRHFMEIRGLGILDIERLYGVG
                     SVKNDEFIELVIELELWDQNKEYDRVGLDEQFTEILGLEISTATIPVRPGRNIAMIIE
                     VATRNFRQKRLGYNAAEELNKRIRERGISRRRQK"
     gene            complement(2269..2460)
                     /locus_tag="VK031_07535"
     CDS             complement(2269..2460)
                     /locus_tag="VK031_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HLS53809.1"
                     /translation="MAKKLYRSREDRMLAGVCGGIGEYFNIDPTIIRLIWAILSIPTA
                     FFGGVVLYIIAAVIIPEKI"
     gene            complement(2441..>3988)
                     /gene="uvrC"
                     /locus_tag="VK031_07540"
     CDS             complement(2441..>3988)
                     /gene="uvrC"
                     /locus_tag="VK031_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HLS53810.1"
                     /translation="IKTRQVKKDGAKYFGPYPSANAVNDAIDILHDVYPIRTCKLNLE
                     KRLENFRPCLEYFIGRCQAPCLGKVDHDEYMEMIDEVIQFLNGRDEKIVNMIEKKMKE
                     ASKNLEFEKASIYRDQLISLEHLQERQKIVSSQLIDQDIIAMARGIEEVCVQVFFIRS
                     GKITGREHFLLDDTFSTERKEVLSSFIKQFYMGSAYVPKEILIEEEIDDAETIGQWLS
                     ARRGSRVNIHVPKRGEKSQLIEMVRKNARDMLNKYGDRFLKKHRENMKALEEIQYILD
                     LEEPINRIEAYDISNISGVQSVGSMVVFEKGEAKKSDYRRFRIREVTSPDDYASMEEV
                     IRRRFLRGVAEKNAAKESGLEMKGFSSFPDLIMIDGGKGQVNVALRVLSDLGVNIPVC
                     GLVKDNFHKTRGIIYNNREYRLDKRSLGFRLIYRIQEEAHRFAISYHRSLRTRDMFRS
                     QLDDIKGIGERRKKALMKHFQSIEKIKNASIEELLEVESMNRPAATNLYNHFRKKGGN
                     KNGKEII"
BASE COUNT         1081 a          891 c          553 g         1463 t
ORIGIN      
        1 aaggagttgt atcctttgaa cctaaaccat atcttccacc tacgattact ggcttttcat
       61 ctctattata gaaaatggta tttacatcta agtgtaatgg ctcagctatt gcaccttttt
      121 ccttggttct atctagaact gctattttct ttactgtttc tggtaaaaca tcgaagaaat
      181 attctgcaac aaaaggccta tataggtgta ccttgattag gcctaccttt tcccctctgt
      241 caactaagta atctatagtt tcttctatag tttgtgttac tgaacccatg gcaacaataa
      301 ttctttctgc ctctggatgt ccgtaatata caaatggctt atattctcta cctgttaatt
      361 cgcttatttt cctcatgtag tcataagtaa cttctccaat ctcatcatag aatggatttg
      421 aggcttctgc agcctggaaa tatatgtctg gattttgtgc cgtacctcta gttactggat
      481 tttctggatt taatgccctt tccctaaact cttgtagtgc ttctttgtct aagagttttt
      541 ctaaatcttc atactctatt acttcaattt tttgtacctc gtgacttgtc ctaaaaccat
      601 caaagaagtg taggaaaggc actcttcctt taatagcagc cagatgggcc actccaccta
      661 aatccataac ttcttgaaca ctaccggatg ctagtaatgc aaatccagtc tgtctagcag
      721 ccataacatc ttggtggtcg ccaaaaatac ttaaggcatg ggaagctagt gctcttgctg
      781 atacatgaaa tacacctggt aatagctctc ctgctatttt atacatatta ggaatcataa
      841 gtaataaacc ttgagatgca gtgtatgttg ttgtcaatgc tcctgcctgt aaagcaccgt
      901 gaactgctcc tgcggctcct gcctcagatt gcaactcttt tactaaaact tcttgaccga
      961 aaatgttttt tcttccttca gctgcccaag catcaacatg ctctgccata tctgaagatg
     1021 gggttattgg gtaaatagct gcaacatcgg taaatgcata ggatacataa gatgcagcca
     1081 tattaccatc catggtcatc atttttttgc ccatagtatg tagtcctcct ttaaatttga
     1141 tttatgaaaa acctttgcat atctagatag ttaaattttc tactaattaa tatgcaataa
     1201 atatgtctta ttttgtactt cctttccgta ccataattag tatattaaac attaaataca
     1261 atgtcaactg atgaaaacgt tttttaagct aaatttttct ttatttgcga aaactcccta
     1321 caaatcctat ttctgccttc ttctggatat acccctttct cttatccttt tattcagttc
     1381 ttcggccgca ttataaccta atcttttttg tctaaagttc ctagtggcta cttctattat
     1441 catggctata tttcttcctg gcctcactgg tatggtggcc gttgaaattt ccaggcctaa
     1501 aatttcggta aattgctcat ctagacctac cctatcatat tccttatttt ggtcccataa
     1561 ctccagctct attaccaatt ctataaattc gtcatttttt acagaaccaa caccatataa
     1621 cctttctata tctaatattc ccaaacccct tatttccata aaatgtctag taatctcagg
     1681 tgattttcca attaatttat cttccaacct ttttattatg actatatcgt ctgagataag
     1741 cctatgtcct ctcatgacca gatctagggc tgtttcagat tttcctactc ctgatttacc
     1801 tgtaattaag actccaaccc cataaacttc tacaaagaca ccatgtaccc tggtttctgg
     1861 tgctaagtag aagttaaggg tatttattgt ttcgttaatt attcttgaag tggacttgtc
     1921 agatctgagt atggttctat catatttttt ggccaaatct attaattccg gaaagactgg
     1981 tagtcctctg gatactataa gggctgggat agtaaagctt aaaaatttat ccagtttttc
     2041 gtacctttca ttactaggta gtttagatag ataggtccac tctgcctttc caattatctg
     2101 aagtctctct ggcacaaatt tatcataatg gccggctatt tgtagtccag gcctacttat
     2161 ctcagatgaa tgtatttcca tatcctctat tgaacttgac ttgcaaataa tttctatatc
     2221 taaatcctca ataatctgta gtaaatctat agaattcatg tctacctcct atattttttc
     2281 tggaattatt acagctgcaa taatatataa gaccacgcca ccaaagaagg cagtaggtat
     2341 ggatagaatt gcccaaatta atctaattat tgttgggtca atattaaaat actcaccaat
     2401 cccaccgcag actccggcta gcatcctatc ttctctggat ctatataatt tctttgccat
     2461 ttttatttcc cccttttttc ctgaaatggt tatataaatt agtagccgct ggcctgttca
     2521 tactttccac ttctaataat tcttctatac tagcattttt aatcttttct atactttgga
     2581 aatgtttcat caaggctttc ttccgccttt ccccaattcc ttttatatca tctagttgtg
     2641 atctaaacat gtctctagtt ctcaaactcc tatgatagct gatggcaaat ctatgtgcct
     2701 cttcctggat tctgtagatt aacctaaacc ctagtgatct tttatccagc ctgtattccc
     2761 tgttattgta tataataccc cttgttttat gaaagttatc ttttactaaa ccgcatacag
     2821 gaatatttac ccctaaatct gataaaaccc ttaaggcaac atttacctgc cccttacctc
     2881 catctatcat tattaaatct ggaaaactag aaaaaccttt catctccagg ccactttctt
     2941 tagctgcatt tttttctgca acccctctta aaaatcttct tctaattacc tcctccatgc
     3001 tggcataatc atctgggctg gttacctccc tgattctaaa tcttcggtaa tcagactttt
     3061 ttgcctctcc tttttcaaaa actaccatag atcctacaga ttgaacaccg ctaatattgg
     3121 atatatcgta ggcttctatc ctatttatcg gttcttccaa atctaatata tattgaattt
     3181 cctccaaggc cttcatgttc tctctatgtt tttttaggaa cctatctcca tatttattta
     3241 acatatccct agcatttttt ctgaccattt ctattaattg tgacttttcc cctcgttttg
     3301 gaacatgaat atttaccctt gaacctctcc tggcacttaa ccattgccca atagtttctg
     3361 catcatctat ttcctcctct attagaattt cctttggcac ataggctgaa cccatataga
     3421 attgttttat aaaggagctg agtacttctt ttctttctgt actaaaagtg tcatctaaaa
     3481 ggaaatgttc tctccctgta attttacctg agcgtataaa aaacacctgg acacatactt
     3541 cttctatacc ccgggccatg gcaattatat cctgatctat aagctgggaa gaaacaattt
     3601 tttgtctttc ttgtaaatgt tctaaagata tcagttggtc tctgtagatg ctagccttct
     3661 caaattccaa gttttttgaa gcttccttca tttttttctc aatcatattg actatctttt
     3721 catccctacc gttgaggaac tggattacct catcaatcat ttccatatat tcatcatgat
     3781 ccaccttacc taaacaaggg gcctgacacc tgcctataaa atattctaaa cagggtctaa
     3841 agttttccaa cctcttctcc aagtttagtt tacaagtcct tataggatat acatcatgca
     3901 aaatatctat agcatcattt acagcattgg cagaaggata aggtccgaaa tattttgccc
     3961 catccttttt aacctgtctg gttttaat
//
LOCUS       DATGSI010000489         5171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_80891, whole genome shotgun sequence.
ACCESSION   DATGSI010000489 DATGSI010000000
VERSION     DATGSI010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80891"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VK031_07545"
     CDS             complement(<1..998)
                     /locus_tag="VK031_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HLS53811.1"
                     /translation="MSNKVIVVGGGAAGMMAAIVARRNGAEVTILERNDRVGRKILAT
                     GNGRCNYTNINLSASNYHGKNPKFVYSALSSFDLYQTLDFFERLGITPAIEDDGRVFP
                     LSFQSSSVLDVLRYEIKDLGVRLITDAQVVDIKKKKDFLVSLKDGRQFRSDKLILATG
                     GKAMPTSGSDGNGYNLARKLGHSITEIFPGLVQLKLDGDLFKQMKGVRFVGKASLYTN
                     DNFLLADKGDILFTDYGISGPPILQLSRTALDCLNKGERVSLKIAVIHTMDKDDLDKY
                     LIQRFKYMPSKPISQALIGLINKRLILPILKELNINKDKNVANISRAHINKLSKLLT"
     gene            complement(1097..1477)
                     /locus_tag="VK031_07550"
     CDS             complement(1097..1477)
                     /locus_tag="VK031_07550"
                     /inference="COORDINATES: protein motif:HMM:NF017701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HLS53812.1"
                     /translation="MANLDPNLLSLEVELYDELPEVERDILFNELHFNRGDVSEYMIR
                     STQSRVKYKGHKFELINPKEIEKGDIIIESSLYTHYAGEMQIALKAMENSGRSSVVGK
                     VVDEEIFLLDQIRPWQKFRLREKI"
     gene            complement(1483..2055)
                     /locus_tag="VK031_07555"
     CDS             complement(1483..2055)
                     /locus_tag="VK031_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HLS53813.1"
                     /translation="MKKTLNKFKEVVNIAKDLGMEVIADVSPDVFKDLNVKINDLKVF
                     KDIGLGGIRVDLGFSGNEESIMSYNQYGLKIELNISGGTKYIDNVMSYLPNKDNIIGC
                     HNFYPHRYSGLSRTHFLEATRRFKQYGLRTAAFVSSQSATFGPWDVKEGIPTLEEHRK
                     LPIGVQAKDLFNTKLIDDIIIANVMLLSRN"
     gene            complement(2036..2176)
                     /locus_tag="VK031_07560"
     CDS             complement(2036..2176)
                     /locus_tag="VK031_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HLS53814.1"
                     /translation="MNKRGISIYSDRSNFDDVKYLNLAAKYGFSRVFINFLTVKDEKN
                     LK"
     gene            complement(2180..2500)
                     /locus_tag="VK031_07565"
     CDS             complement(2180..2500)
                     /locus_tag="VK031_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HLS53815.1"
                     /translation="MELELIIMNIINHSGEARSLSMEAITLAKQGDIVGAEKNIEEAN
                     ERLREAHKSQTQLIQGEARGEKNQISLLLVHAQDHLMNALTVKDMAIEFIDLYRSIQD
                     EKGN"
     gene            complement(2683..3348)
                     /locus_tag="VK031_07570"
     CDS             complement(2683..3348)
                     /locus_tag="VK031_07570"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53816.1"
                     /translation="MAHILNLDPARVRIDFAGLNHLVYGFNIYVDYIDKTEEMFNKFL
                     ASRQDISMKNIGLQDWDRNFLKALNLLPCPYHKYYYKKEDMLKEQLEEFNKGKTRALE
                     VMETEKELFSIYQNPKLAEKPKELELRGGAYYSDVACNLILSIYNDKGDIQVVNTINK
                     GTIKDFPDHYALEISSYISRQGPVPCQFIDEFPLKIKNLIHQIKAWEILAAEAAVEGD
                     YNK"
     gene            complement(3410..3964)
                     /locus_tag="VK031_07575"
     CDS             complement(3410..3964)
                     /locus_tag="VK031_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HLS53817.1"
                     /translation="MNIIDKDHFVQSIKDMKKRCNIIGIVSPLEISIPGIPFIPAIDI
                     FTKRGEVLLKELVEDDEVYRKIKESIYKHLKNVKDTDLIDNIRYVIKNIEALLKIKIA
                     RDVEMGIVLHIAFLVDSLVGGSRPRRFEGLKNFKLAHGNKMALIKNSFQFMEDKYQVD
                     LGEDEIAYILKLFIATGSLYDLIY"
     gene            complement(3976..4476)
                     /locus_tag="VK031_07580"
     CDS             complement(3976..4476)
                     /locus_tag="VK031_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLS53818.1"
                     /translation="MRKVDEIYRIILELEGEKNQGVTTSQVADLAGIARSNASRYLNQ
                     LHNRNKLEKIEGRPVLYKLKDKDDLSLGSNLDKEKTNYSFNKMVGADQSLSSDIEQAK
                     SAIMYPPKGLHTLILGETGVGKSLFAEQMYNFALESKVLDQDTPFIRFNCADYTDNPA
                     LLTAQT"
     gene            complement(4667..>5171)
                     /locus_tag="VK031_07585"
     CDS             complement(4667..>5171)
                     /locus_tag="VK031_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HLS53819.1"
                     /translation="GLNRTLLGDGLATMFAAAVGGPANTTYGENTGVLAITKNYDPSV
                     LRLAAMFAIALGFVAKIGGVLASIPEAVMGGISMMLFSMIALIGVKTIRNEKVEFNFK
                     NTLIMGTILVIGLGSDFLSQLLNINIGISLNETVSISGLSFAALVGIGLNLAISLMEM
                     LKNKKEI"
BASE COUNT         1504 a         1084 c          678 g         1905 t
ORIGIN      
        1 gttaataact ttgataattt atttatatgg gccctactta tattggccac atttttgtct
       61 ttattaatat ttaattcctt gagaataggt agaattagcc ttttgtttat tagcccaatt
      121 agggcctgtg atataggctt acttggcata tatttgaatc tttggattag atatttatct
      181 aaatcatcct tgtccattgt atggataaca gcaattttaa gactaactct ttcaccttta
      241 tttaaacagt ctagggcagt cctactaagc tgtaagattg gaggcccact tattccataa
      301 tcagtaaata aaatatctcc cttatccgcc aacaagaaat tgtcattagt ataaagggaa
      361 gccttaccaa caaacctgac acctttcatc tgcttaaaca gatccccatc taattttaat
      421 tgtaccaggc ctgggaatat ttctgtaata ctatgaccca gcttcctggc caaattatat
      481 ccatttccat ctgacccact tgtaggcatg gcttttccac ctgttgccaa tattaattta
      541 tcgctcctaa attgtctgcc atcttttaaa ctgacaagaa aatctttctt tttcttaata
      601 tccactacct gggcatctgt tataagcctt actcccaaat ctttgatttc atatcttaaa
      661 acatctaaaa cacttgaact ttgaaaggat aaaggaaaca ccctaccatc atcttctatg
      721 gcaggagtta tgcctagccg ttcaaagaaa tcaagggttt gatatagatc aaaactagat
      781 agagcactat agacaaattt agggtttttc ccatgataat tactggctga tagattaatg
      841 ttggtatagt tacatcttcc attgccagtg gctagtattt ttctacctac cctatcattt
      901 ctttctaata ttgtaacttc tgcaccattc ctcctggcca caatagcagc catcattcct
      961 gcggctcctc cgccaactac aataacctta ttagacattt tctactcctt ttttaaagtc
     1021 ttcttctatt ttatacttaa aaagttattt tgtccataaa atgaaaaaac tgtgtattaa
     1081 tacacagttt tttatattat atcttttccc ttagtctaaa tttctgccaa ggccttatct
     1141 gatctagaag gaaaatctcc tcatctacca cttttcctac tacagaggat ctgccactat
     1201 tttccatagc tttaagagct atctgcattt ctcctgcata atgggtataa aggctgctct
     1261 caattataat gtctcccttt tctatctctt taggatttat tagttcaaac ttatgacctt
     1321 tatatttcac cctactttgt gtggatcgta tcatatattc tgatacatct cccctgttaa
     1381 aatgtagctc attaaataga atatctctct ccacttctgg cagctcatca tataactcaa
     1441 cctctagact taatagatta gggtctagat tagccagtgt ttttaattcc tgctcaacag
     1501 cataacattt gctattatta tatcatctat tagcttggtg ttaaataaat cctttgcttg
     1561 gacacctatt ggaagctttc tatgttcctc caaggttggt ataccttctt ttacatccca
     1621 aggtccaaag gtggcagact gggatgatac aaaggctgca gtccttaatc catattgttt
     1681 aaaccttcta gtagcctcta aaaaatgggt ccttgacaga cctgagtacc tatgggggta
     1741 gaaattgtga cagcctataa tattatcctt atttggtaaa tagctcataa cattatcaat
     1801 atatttagtg ccaccactta tatttaactc tatttttaaa ccgtattggt tatagctcat
     1861 aatactttct tcattgccac taaaacctag gtctactctt atgccaccaa gacctatatc
     1921 cttaaatact tttaaatcat ttatcttaac atttaggtcc ttaaacacat ctggagatac
     1981 gtctgctatt acctccatgc ctaaatcttt ggcaatattt actacttcct taaatttatt
     2041 taaggttttt ttcatcctta actgttaaaa agtttataaa tacccggcta aaaccgtatt
     2101 tagcagcaag attcaaatac tttacatcat caaaatttga tctatcagaa taaatagaga
     2161 ttcctctttt attcataaat caattcccct tttcatcttg tatgctccta tacaagtcaa
     2221 tgaattctat agccatatcc ttaacagtta aagcattcat cagatgatcc tgggcatgaa
     2281 ctaataataa agaaatttga ttcttttctc cccttgcctc cccttgtatt aattgagttt
     2341 ggcttttgtg agcctctcgt aacctttcat tggcctcttc tatatttttt tccgcaccga
     2401 caatatcccc ctgtttagca agggttatgg cctccataga caggcttcta gcttctccac
     2461 tatgatttat aatattcatt attattagtt ctaattccat tatgatctct cctcgtacaa
     2521 tataatataa tctgttcaaa tataaggcta ttttaacata atttaacttt tctggtcaaa
     2581 aaaattaggt aaatactttt gatggctaga tagtagttta tcaaagacca tccttcctgt
     2641 gctgtcattt tgaactaaag gattagatat cattgataaa agtcacttat tataatctcc
     2701 ttctactgct gcttctgctg ccaatatctc ccaggccttt atctgatgaa tgaggttctt
     2761 tatctttaat ggaaattcat cgataaattg acagggtaca ggtccttgtc tacttatata
     2821 ggaacttatt tccaaggcat aatgatcagg aaaatcctta attgtccctt tattaattgt
     2881 atttactacc tgtatatctc ccttgtcatt atagattgat aagattaaat tacaagccac
     2941 atctgaataa taggctcctc ctctcagttc caattctttc ggtttctctg ccaatttagg
     3001 attttgatat atgctgaata attctttctc tgtttccatc acttccaagg ccctggtttt
     3061 acctttatta aactcttcta attgctcctt caacatatct tctttcttat aataatattt
     3121 atggtaaggg caaggcagta ggtttaaggc ctttaaaaaa ttcctatccc aatcctgcag
     3181 gccaatattt ttcatgctta tatcctgtct acttgctaga aacttattaa acatttcctc
     3241 agttttgtct atgtaatcta cataaatatt gaaaccatat actaaatggt ttaatcctgc
     3301 aaaatcaatc ctaactctgg ctggatctaa gttaagaata tgggctattt ctcattccat
     3361 actaacaggt acattacata aacctataaa tttcctatgg gaggaatatc taataaatta
     3421 aatcgtataa gctacctgtt gctataaata attttaatat ataggctatt tcatcttccc
     3481 ctaagtccac ctggtattta tcttccataa attgaaagct gttttttatc aaagccattt
     3541 tattcccatg agccaattta aagtttttca aaccttcaaa tcttctaggc ctacttcctc
     3601 ctaccaggct atcaactaaa aaggcaatat gaagcactat gcccatctct acgtcccttg
     3661 ctatttttat ttttaatagt gcttctatat ttttgattac atatctaata ttatctataa
     3721 gatctgtatc cttcacattt ttcaaatgct tgtatataga ttctttgatt tttctatata
     3781 cttcatcatc ttcaactagt tcctttaata aaacttcccc tcttttagta aagatatcta
     3841 tggctggtat aaaaggaatt ccaggaatac taatctccaa tggcgatact atccctataa
     3901 tattacatct ttttttcata tcttttatac tttgaacaaa atgatccttg tcaattatat
     3961 tcattggtat tagatctatg tttgtgccgt taatagggct ggattgtcag tataatcagc
     4021 acagttaaat ctaataaaag gagtatcttg gtctaatacc tttgactcta aggcaaaatt
     4081 atacatttgc tcagcaaata aggatttgcc taccccagtt tctcccaata ttagggtatg
     4141 aagtccctta ggtggataca taatggcaga cttggcctgt tcaatgtccg aagataagct
     4201 ctggtcagct cccaccattt tattgaaact atagtttgtt ttttctttgt ccaagttact
     4261 tcctaggctt aggtcatctt tatctttcaa tttatataaa actggtcgcc cctctatctt
     4321 ttccaatttg ttcctgttat gcaattggtt aagataacga ctggcatttg atctggcaat
     4381 accggctaaa tctgccactt gtgaggtagt tactccctga ttcttttccc cttctaattc
     4441 taaaatgatt ctataaatct catctacctt cctcatatcc atccccctaa gtctaaagct
     4501 ttcataaaac cttcttgctt caaatcatct aaactccatt ttacttccga tatctatttg
     4561 tactttaact tttattaata aatttgtcta tataaaggtt actcaccaaa ttggatagcc
     4621 taattcatac caataaaaaa ggcctacctc ttggtagacc ttttaattat atttcttttt
     4681 tatttttcaa catttccatt aatgagattg ctaagtttaa tcctattccc accaaggctg
     4741 caaaacttaa gcctgatatg gaaacagtct catttaagct tatccctata tttatattta
     4801 gtagctggct taaaaaatca ctgcctaggc ctattaccaa aatagttccc attattagag
     4861 tatttttaaa gttaaactct actttttcat ttctaattgt cttaacacct attaaggcta
     4921 tcatactaaa tagcatcata gatattccac ccattaccgc ttctggtata gaagctagaa
     4981 ctccaccaat tttagctaca aatcctagtg caattgcaaa catggctgct agccttaaaa
     5041 ctgaaggatc atagttctta gttattgcca gtacacctgt gttctctcca taggtggtgt
     5101 tggcaggccc tcctactgct gctgcaaaca tggttgcaag cccatctcct aagagagtcc
     5161 tgtttaaacc t
//
LOCUS       DATGSI010000490         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_363516, whole genome shotgun sequence.
ACCESSION   DATGSI010000490 DATGSI010000000
VERSION     DATGSI010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363516"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VK031_07590"
     CDS             <1..792
                     /locus_tag="VK031_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53820.1"
                     /translation="KQAVVFLCLFNQYGGVRMNPFIPRKKANVVLVAGNAGIEIFSSL
                     KNLDLQVIPTIKCEDVYKSIAYHPDIVIHPVNHKTLVIAPNVFDYYKETLKGLDLRLI
                     KGEKKLGRKYPDTIAYNVGRIGNKAIHNFKYTDEVLKFYLEKEKIEFINIKQGYSKCS
                     LAIAGEKGIITSDPPMYKKLKSLGYNSLLIKPGHIYLEGQEYGFIGGATGNLSQNTIL
                     FSGHLNQHPNQWEIIKFLLKNKLKIKYLSKEEISDIGSIISLYCN"
     gene            857..1702
                     /locus_tag="VK031_07595"
     CDS             857..1702
                     /locus_tag="VK031_07595"
                     /inference="COORDINATES: protein motif:HMM:NF020391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HLS53821.1"
                     /translation="MDPVTIGSTIPAEKMYPIFDRMPFKKRVNINYKEYKDRFEYNFS
                     LPKKKLNEDITFTNIMANLAQDMIMKFYIKDLVSQRVTKIIGKMDYANKVKIIDEVYK
                     VVLEDELFVYEKEKIYKEIFEYLIENNILIIDGYLKFRSDSFLDLVDKAVEFVLSHIQ
                     IDMEYNEFISTLKHFVETQSSEIDLVNLVLEKGDFQLLDKTHQKIDNDHITELLQDIF
                     LDEVNKSDILLSSLIGLSPKEIIIHEKEKEEGLVDTLKMIFTDRIKLCPGCSLCHLKI
                     SNEKD"
     gene            1787..>2672
                     /locus_tag="VK031_07600"
     CDS             1787..>2672
                     /locus_tag="VK031_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HLS53822.1"
                     /translation="MSGKIVKPSILPGFMELLPEEQLIFNQMLDTIRNNYEKFGFLPI
                     DTPVIERSEVLLAKGGGETEQQVYRFERGNTDMALRFDLTVPLARYVAQYYSDLNFPF
                     RRYHIGKVYRGERNQRGRYREFYQCDIDIIGNMELDIINDAEIPALIYSTFKDLNIGD
                     FTIKINNRKLLKGLLESLKIKDGTFVLRAIDKLEKIGVSQVKKELEDLGLLDEAIEQI
                     LGFINIKGTNKEIIKQLENLEVANKDFKLGLEEIRTVTKYISLFGVPEKYYKIDLTTV
                     RGLDYYTGTVYETILDDYP"
BASE COUNT         1044 a          328 c          500 g          800 t
ORIGIN      
        1 aaacaggcag tagttttttt atgtttattt aatcaatacg gaggagttag gatgaatccc
       61 tttattccaa gaaaaaaggc caatgtggtc ctggtggcag gaaatgcagg aattgaaata
      121 ttttcaagct taaaaaacct agatcttcag gtaattccta caataaaatg tgaagatgta
      181 tataagtcaa ttgcctatca tccagatata gtaatccatc ctgtaaatca taaaacccta
      241 gtcattgccc caaatgtttt tgactactat aaggagactt taaaaggatt ggacttaagg
      301 ttgattaagg gagaaaagaa attaggaagg aagtatcctg atactattgc atataatgta
      361 gggaggatag gaaataaggc tattcataat tttaaatata cagatgaagt attaaaattt
      421 tatttagaaa aagaaaaaat agaattcatc aatataaagc aaggttactc taaatgcagt
      481 ttagccatag caggagaaaa ggggataatt acatctgatc cacctatgta taaaaaatta
      541 aagagcttag gctacaatag cctattgatc aagccaggcc atatttattt agagggacaa
      601 gagtatggat ttataggtgg cgccaccggt aatttatctc aaaatacaat cctattttca
      661 ggacatttaa atcaacatcc caaccagtgg gagattataa aatttttatt aaaaaacaaa
      721 ctgaaaatca aatatctatc aaaggaagaa atatcagata taggaagcat aattagtctt
      781 tactgtaatt agtgtcaaat ctttactgca tatgtgaata taaagggtac atactagtag
      841 agaagggagt gatattatgg accctgtaac cattggtagt acaatcccag cagagaagat
      901 gtaccctata tttgatagaa tgccctttaa aaaaagagtt aatataaatt ataaggagta
      961 taaggatagg tttgaatata atttttcttt gcctaaaaag aaattaaatg aagatattac
     1021 ctttaccaat attatggcca atctggccca agatatgatt atgaaatttt atataaaaga
     1081 tttggtgagc caaagggtta ctaaaataat tgggaaaatg gactatgcaa acaaggtaaa
     1141 gataatagat gaggtatata aggtagtttt agaagatgaa ttatttgttt atgaaaaaga
     1201 aaagatttat aaggagatat ttgaatattt aatagaaaat aatattttga ttatagacgg
     1261 ctatttaaaa tttagatcag attccttttt agatttagta gataaggcag ttgaatttgt
     1321 cttaagccat attcaaattg atatggaata taatgaattt ataagcacct tgaaacactt
     1381 tgtagaaacc caaagttcag aaatagacct ggtaaatttg gtactggaaa aaggagactt
     1441 tcaactgtta gacaaaacac atcaaaaaat agacaatgat catattactg agcttttaca
     1501 agatattttt cttgatgaag taaataaatc cgatatcctg ttaagctcat taatagggct
     1561 atcacccaaa gaaattataa tccatgaaaa agaaaaggaa gaaggacttg tggatacttt
     1621 aaagatgatt tttacagata gaattaaact ttgtccagga tgtagcctat gccatttaaa
     1681 aatctccaat gaaaaggatt aaagagttga tttatcaact ctttttcttt gacaattttt
     1741 gaattaatgt tattattaaa tgagagataa aattggaggg tttttaatga gtggaaagat
     1801 tgttaaacca agtattctac caggatttat ggaattatta ccagaggaac aattaatatt
     1861 taatcaaatg ctagatacta taagaaataa ttatgaaaaa tttggttttc tgccaattga
     1921 tactccagta atagaaaggt cagaagtact attggcaaag ggcggaggag agacagaaca
     1981 acaggtatat aggtttgaaa ggggcaacac tgatatggcc ctgagatttg acctaactgt
     2041 acccttagcc agatatgtgg cccaatatta ttcagactta aactttccct ttagaagata
     2101 ccatataggt aaggtatata gaggcgaaag aaaccaaagg ggccgatata gggaatttta
     2161 ccagtgtgat atagatatta tagggaatat ggaattggat ataattaatg atgcagaaat
     2221 ccctgctctt atatattcaa cctttaagga tctcaatata ggtgacttta ccataaaaat
     2281 taacaataga aaattattga aaggtttgct tgaaagctta aagattaagg atggtacctt
     2341 tgttttaagg gctattgaca agctagagaa aattggtgtc agccaagtta agaaggaatt
     2401 agaagactta ggacttttag atgaggctat tgagcaaatt ttagggttta taaatataaa
     2461 aggaacaaat aaggagataa ttaaacaatt agaaaaccta gaagtagcca ataaggattt
     2521 taaattaggt ttagaggaga taaggacagt tactaaatat ataagccttt tcggggtacc
     2581 tgaaaaatat tacaaaatag acctgacaac agtaagaggg ctggattact atactggaac
     2641 agtttatgaa acaatacttg acgattaccc at
//
LOCUS       DATGSI010000491         2846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_649163, whole genome shotgun sequence.
ACCESSION   DATGSI010000491 DATGSI010000000
VERSION     DATGSI010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649163"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VK031_07605"
     CDS             complement(<1..236)
                     /locus_tag="VK031_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HLS53823.1"
                     /translation="MSEIINNREYRQEQLKEVIRELHAGKSVEEVKEKFAAVIDGVSP
                     REIGEMEVQLVKEGLPIEDIQYLCDVHAAVFKGS"
     gene            complement(322..>2846)
                     /locus_tag="VK031_07610"
     CDS             complement(322..>2846)
                     /locus_tag="VK031_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HLS53824.1"
                     /translation="KERYTGCGSSILRRDELDNFQYRSRQGQCGRCVNNCLLTINIFS
                     NRERYLTGNRCERGVGIKKELTELPDLYDYKYKRTFNYKSLPLDKAPRGTVGIPRVLN
                     IYENYPFWHRFFTELGFRVELSKESSRRVYEIGLASIPSETACYPAKISHGHIIDLIN
                     KDIKFIFYPSVFYEEKEDHLSDNHLNCPVVTGYPEVIKHNIQELRDKDILFLNPFISF
                     DNKNSLRRELHKSLKVFNISKREINKATEKAWEEMYRYKLDIRLKGEETLKYIKEKKI
                     KGIVLAGRPYHIDPAINHGIPNLINSLGLAVLTEDSIDHLEGLPQPLRVLDQWTYHSR
                     LYRAAAYVSKSEDLELIQLNSFGCGLDAVTTDQVQEILQSRHKIYTQLKIDEVSNLGA
                     AKIRVRSLLAALQEKGRQIKTEADKLYQDRVIFTKENRKKHTILIPQMAPIQFKLIER
                     VLNTSGYQVEVLDQVDARAVDEGLKYVNNDACYPSIIVIGQFIAALKSGKYDLDNTSL
                     LISQTGGVCRASNYVGFLRKALKEAGFEHVPVISVSVQGIEKNPGFTFSYDMVKRGIM
                     ATLLGDLLMRVSLRVRPYEKIKNSTNLLLNRWIDKLIASLDNINQAQFIKYANDIVTS
                     FDKIEIIDTPKPKVGIVGEILVKYHPVANNQLIEILEEEGAEVVLSDLTDFLLYCLYN
                     ADFKYKALGQGLKGKLIGDIGIKYIEYYRKHIRKALNKSQRFTPPLTIEELGERAGKL
                     ISLGNQSGEGWLLTAEMLELIEHGAENIVCVQPFGCLPNHITGKGMIKAIREIYPKAN
                     IIPIDYDPGASAVNQLNRLKLMMATAEENIKEKNEIEATVAK"
BASE COUNT          753 a          600 c          444 g         1049 t
ORIGIN      
        1 aagctaccct taaatacagc tgcatgtaca tcacataagt attggatatc ttctataggg
       61 aggccttcct tgaccaattg gacttccatt tctcctattt ccctaggaga tacaccatca
      121 attactgctg caaatttttc cttaacttct tccacagact tgcctgcatg cagttctctg
      181 attacctcct ttaattgttc ttgtctatat tcacgattat taataatttc gctcatatta
      241 ctttcctcct atacatttaa ttaatatatt aatacccatg atctaagcaa cttaaatcat
      301 gggtattaat cttcttatat tttattttgc tactgtagcc tctatttcat tcttttcttt
      361 aatattttct tctgcagttg ccatcattag cttcaaccta tttaattgat ttactgcact
      421 ggcacctgga tcgtagtcta taggtataat atttgccttt ggatagattt cccgaatggc
      481 cttaatcatt cccttaccgg ttatatggtt aggcagacaa ccaaagggtt ggacacaaac
      541 tatattctct gctccatgct ctattaattc caacatttct gcagtaagta gccagccctc
      601 tcctgattgg ttccctagag atattagttt tccagccctt tcccccagtt cctcaatagt
      661 aaggggagga gtaaatcttt ggcttttatt taaggccttt ctaatgtgtt ttctatagta
      721 ttcaatatat tttataccta tatctcctat tagtttccct ttcaatcctt ggcctaaggc
      781 cttatattta aagtcggcgt tatataaaca gtataggagg aaatctgtta aatcgcttag
      841 tactacttct gctccctctt cttctagaat ttctattagt tggttattgg ctacaggatg
      901 atatttgact aatatttccc caactatgcc taccttaggc ttaggagtat ctataatttc
      961 tatcttgtca aaggatgtaa ctatatcatt ggcatattta ataaattggg cctggtttat
     1021 attgtctaag gaggcaataa gcttatctat ccacctattt aataagaggt tggtagaatt
     1081 ctttatcttc tcatagggcc ttaccctaag gcttactctc ataagtaaat ctcctaaaag
     1141 ggtggccatg attccccttt ttaccatatc ataggaaaag gtaaagcctg gattcttttc
     1201 tattccttga acacttacac tgattactgg tacatgttca aaacctgcct cctttaaggc
     1261 ctttcttaag aagcctacat agttagatgc tctgcacact ccccctgttt gagatattaa
     1321 aagagaagtg ttatcaagat catattttcc agattttaag gcggctataa actgaccaat
     1381 tactataata gatgggtaac atgcatcatt atttacgtat tttaaccctt catctacagc
     1441 cctggcatct acctgatcta aaacttccac ctggtaacct gaagtattta aaactctctc
     1501 tatcagttta aactgaatag gtgccatttg tgggattagg atggtatgtt ttttcctatt
     1561 ctccttagta aatataaccc tgtcctgata aagcttatcc gcttccgttt ttatctgtct
     1621 gcccttctcc tgtaaggccg ccagcaagga tctaaccctt attttggctg cccccaggtt
     1681 acttacctca tctatcttaa gctgggtata gattttatgt cggctttgta aaatttcttg
     1741 aacctggtct gttgttacag catctaggcc acagccaaag gaatttagct ggattagttc
     1801 taaatcttca ctttttgata cataggctgc cgccctatat aaacgagagt gataggtcca
     1861 ttggtccaag actcttaaag gttgtggtag cccttctagg tgatctatgc tatcttccgt
     1921 tagcactgcc agccctaggg aatttattaa gtttggtatc ccatggttta tagcaggatc
     1981 tatatggtag ggcctgcctg ccagcacaat ccctttaatc tttttttcct taatatattt
     2041 aagagtttct tctcccttta accttatatc cagtttatac ctgtacattt cttcccaggc
     2101 cttttctgtg gccttattaa tttctctttt gcttatgtta aagactttta aagatttgtg
     2161 taattctctt cttagactat ttttattgtc aaagctgata aaagggttta aaaataaaat
     2221 atccttgtcc cttaattctt gaatattatg cttaattacc tcagggtaac cagtaactac
     2281 tggacaattt agatggttgt ccgatagatg atcctctttt tcctcataaa aaactgatgg
     2341 ataaaagata aatttaatat ccttatttat aagatctata atatggccat ggcttatttt
     2401 tgcagggtaa caggcagttt cactggggat agaagctaat cctatttcat aaaccctcct
     2461 gctggattcc ttagataact ctaccctaaa acctagctct gtaaagaatc tatgccagaa
     2521 gggataattt tcataaatat ttaaaactct aggtatgcct actgttcctc taggcgcctt
     2581 atccaggggt agggacttgt aattaaaggt tcttttatac ttgtagtcgt ataaatctgg
     2641 tagttctgtt aattcctttt taatacctac tcctctttca catctattgc ctgttaaata
     2701 tctttcccta ttggaaaaga tatttatggt cagtaggcaa ttgttgacac atctgccaca
     2761 ttgcccctgt cttgacctgt attgaaagtt atccagctca tctctcctta atatactaga
     2821 gccacatcct gtatatcttt ctttag
//
LOCUS       DATGSI010000492         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_649191, whole genome shotgun sequence.
ACCESSION   DATGSI010000492 DATGSI010000000
VERSION     DATGSI010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649191"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..873)
                     /locus_tag="VK031_07615"
     CDS             complement(178..873)
                     /locus_tag="VK031_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HLS53825.1"
                     /translation="MYGSYFLGYGGGSWTLFLFAAILFASYAQMKVSSAYNRYSRISN
                     GTGYTGAQIARMILDRNGLHHVRIETTPGRLTDHYDPRERVLRLSAPIYNGSSVASMS
                     VAAHEAGHAIQHDRGYFMLVLRNNLAPIVGLGARFVWLFIILGFLVSPFLADIGIALF
                     LGVVIFQIVTLPVEFDASRRALVQLENGISPRDRMHASRDMLNAAALTYVASTLVAIG
                     QLLRLLAITNRRR"
     gene            complement(893..>1552)
                     /gene="fmt"
                     /locus_tag="VK031_07620"
     CDS             complement(893..>1552)
                     /gene="fmt"
                     /locus_tag="VK031_07620"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HLS53826.1"
                     /translation="LKKELLDIPKYGCINIHASLLPKYRGAAPINWAIINGEKETGIT
                     IMEMEEGLDTGPIMKKRSIPILPEDNSGTIHDKLALLGGELILETIDDISKGKAIKTP
                     QDHSKSTYAPMIYRDTGKINWNDTGTNIKNLVRGVNPWPGAYTIYEGQTMKIFNIALI
                     SGRKEGKAGQIVKVSQEGIYVNTGDNTAIIKELQFPNKRRMTVREYLAGNEIKLGSIL
                     K"
BASE COUNT          441 a          343 c          232 g          536 t
ORIGIN      
        1 gatctcttga atctattttg tccactaata atttgtttat ctcaatattt gagtaggccc
       61 tttccttatt tatatctacc aggattttga aggccaattc tcttgcattt gaagccatcc
      121 tattcaccct tatcaattaa atatataaaa aataaaaggg gtagtcaccc ccttggttta
      181 tcttcttcta tttgtaattg ctaataatct taacaattgt cctatggcta caagtgtgga
      241 ggctacatag gttaaggcag ctgcatttaa catatctcta gatgcatgca tcctatccct
      301 aggagaaatt ccattttcca gttggaccag ggcccttcta ctggcatcaa actccactgg
      361 cagtgtaact atttgaaaga ttacaactcc taaaaataag gcaatcccta tatctgctaa
      421 gaagggactt accaaaaaac ctagtataat aaagagccat acaaaccttg ctcccaagcc
      481 tactattggg gccagattat ttcttaaaac cagcatgaag tagcctctat catgttggat
      541 agcatgacct gcctcatggg ctgccacact catggaagcc acactacttc cattataaat
      601 aggagcagat aatctcagaa ccctttctct tggatcataa tgatcagtta accggcctgg
      661 agttgtttct attctaacat ggtgtaaacc atttctatct aatatcattc tagcaatttg
      721 agcacctgta taacctgtac cattgctgat tcttgaatac ctattgtaag cgctgctaac
      781 cttcatttgt gcataagatg caaatagaat ggctgcaaag agaaataagg tccaagaacc
      841 tccaccgtaa cctaaaaaat aacttccata catagttgct cacctctcaa ctctatttta
      901 aaatactacc taattttatt tcattaccag ccaaatactc cctaacagtc attctccttt
      961 tgtttgggaa ttgcaattct tttataattg cagtgttatc tccagtatta acgtagatac
     1021 cttcctggga taccttcaca atttggccag ctttaccctc ttttctccct gaaatgaggg
     1081 ctatattaaa aattttcatt gtctggcctt catatatggt ataagctcct ggccaaggat
     1141 taactcctct taccaggttc tttatatttg ttcccgtatc attccagttt attttcccag
     1201 tatccctata tatcatagga gcatatgttg atttactatg gtcctgagga gtttttattg
     1261 cctttccctt acttatatca tcaattgttt ctaagataag ctccccacct aaaagagcca
     1321 gcttatcatg gattgtacca ctattatctt caggtaaaat aggtattgac ctctttttca
     1381 taataggtcc agtatctaaa ccttcttcca tttccataat ggttatacct gtttcttttt
     1441 ctccatttat tatggcccaa tttattgggg ctgctcccct gtattttggc agtaaggaag
     1501 catggatatt tatacagcca tatttaggta tgtctaataa ttctttcttt aa
//
LOCUS       DATGSI010000493         6180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_265842, whole genome shotgun sequence.
ACCESSION   DATGSI010000493 DATGSI010000000
VERSION     DATGSI010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265842"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VK031_07625"
     CDS             <1..349
                     /locus_tag="VK031_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HLS53827.1"
                     /translation="IDAGSSTLDNMISNTEYGLYAKKLGGGSVNPATGDFNFAVMEGY
                     IIENGKILKPVRGATLIGNGIKVLKLIDMVGNNLALGQGMCGASSGSIPANVGQPSIR
                     VKSLTVGGRKGDK"
     gene            351..1694
                     /locus_tag="VK031_07630"
     CDS             351..1694
                     /locus_tag="VK031_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HLS53828.1"
                     /translation="MRYRGLLNQLLYKAKDEFEESEAFLIENKEVNISLYKGEIDKFS
                     IAESGGLSLRGTRAGRMGYAYTEKIDEEALDILIEEASENRKYIDDKEPEVIFEGSKS
                     YKSQDNYSQALEEVGTEEKINFLKKLEAEALRLDKRVFSVNNCGYQEFYQNRHILNSK
                     GVDLEDRTNGAFAFISVVVKEGEDTKTGLSYRIVKDFSELDYKKMAKEAVKNASSMLG
                     AESIKSDNYPIVFENKTFANFLSAFSSIFSAENVQKGLSGLKGKLGEDIANPILTIVD
                     DPFLKDGFASKNFDDEGTSTQVTKLIDKGKLSSLIYNWKAAKKDKVQSTGNASRSYKG
                     QISISPTNLYVQKGETEFTGLIDLKEGVFINDLQGLHSGLNPVSGDFSLSAAGFMIED
                     GQLGKPVNQITIAGNLYELLKDIEAIGKDLEFSLPGASYIGSPSVRVSSLSVSGK"
     gene            complement(1741..2478)
                     /locus_tag="VK031_07635"
     CDS             complement(1741..2478)
                     /locus_tag="VK031_07635"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HLS53829.1"
                     /translation="MKKIFSLIIIFTLIVAITGCESQSSLQGDSPSNNQDGYPEILED
                     KSNEGEITDSQMPQVDEQRDDSNKNTDDLNTPVSDAEQPISNSNSKSVAEEAEIIVDP
                     TPEIKSKEVNLYFANMEYIETGNEDLKHLIPEKRLVKYGDISLAEVVVRELMKGSQNP
                     KLGSSIPTTVKLLEVEVADDTAYVNFAREGLHGGSLQETFTINQIVTSLLELDNINKV
                     QFLVDGKRAESLMGHITVLEPFDSQFK"
     gene            complement(2580..3923)
                     /locus_tag="VK031_07640"
     CDS             complement(2580..3923)
                     /locus_tag="VK031_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLS53830.1"
                     /translation="MAFLFFSINILNNYKTTSIKSEETRLFQTANIVADLYRENMDDI
                     IYLNNMIRDYAKRIDARILVLNDNKTVLIDSNNDLYGQFINNKEIESSLNNHPGSDIY
                     NIDEKKILQATVPIRINHQGQEQIRGVVLISKSISFLWENISNLRKDIIKVSSIALFI
                     AIFLIAITTNTLTKSLRELSKGVSAISGGSLGYKVEHKSKDEVGELIDTFNKMSTSLY
                     DIEANRKLLINSVSHELRTPLTSIEALIDSLLIGENDIRVYKEFLKDIKNETARMKSL
                     INHLTQSIKLEDINLNLTYQDLGKLLEETVKFITPYANKNQVDISLDLDGNIKVYCDR
                     DKLKEVFINLIENAIKYKDKNKKNPMVQISLREEKNSAQIVIKDNGIGIDKSDIPAIF
                     KREFRRINSALTEPYVEGQGIGLAIVKKIVDKHQWKIHVESSLGQGSQFIITIPK"
     gene            complement(3955..4641)
                     /locus_tag="VK031_07645"
     CDS             complement(3955..4641)
                     /locus_tag="VK031_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLS53831.1"
                     /translation="MIGINILIVEDEKLIAKGLKRSLEQQNYKVFVAYDGHQAINTVK
                     NEEIDFIILDLMLPDIDGMMLCKKIREDYDMPILMLSAKDGDYDKILGFEFGADDYMT
                     KPFNTLELIARIKAISRRVESKVRKVAGLIKQGDMVINLEERIVTIKDREVVLTAKEF
                     DMLHLLAKNPGRVYTREEIFSLIWGEEALDVRTIDVHIKNIREKIERNVKEPEYIMTK
                     WGVGYYFNKI"
     gene            5162..5884
                     /locus_tag="VK031_07650"
     CDS             5162..5884
                     /locus_tag="VK031_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS53832.1"
                     /translation="MLKIKNVSKSYAGGTIKAVDNISLEVKAGEIFGFLGPNGAGKTT
                     TIKMIVGLLKPDEGTIMIKDLDVWDKPLKAKSKISYVPDNPAVYDKLKGIEYLNFIAD
                     MYEISKEERKDKIERYTKLFRIEDALNDMIGSYSQGMKQKLVLTSALLNNPDLFILDE
                     PMVGLDPKSSFNLKEIMKAMCEEGRTVFFSTHVLEVAEQLCDRIAIINKGKIITTGSM
                     EELRADAKERESLEKIFLELTE"
     gene            5884..>6180
                     /locus_tag="VK031_07655"
     CDS             5884..>6180
                     /locus_tag="VK031_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53833.1"
                     /translation="MEKLLSLIKIDLLNTFGLSSLVHSMKDRRTRWQFIILAIAFLSL
                     IPSYVMFVKGLSNIYVAYDQLGQRSYFLQLGIFLAQMAVFLFGILYVMSKYYFAN"
BASE COUNT         2005 a         1007 c         1110 g         2058 t
ORIGIN      
        1 tatagatgct ggaagctcca ccttagataa tatgatatct aatacagagt atggcctata
       61 tgccaaaaaa ctaggtggag gtagcgtaaa cccagctact ggtgatttta actttgctgt
      121 aatggaaggt tatataattg aaaatggtaa gattttaaag ccagttaggg gggctaccct
      181 aataggaaat ggaataaaag ttttaaaact aatagatatg gtaggcaaca atttggctct
      241 aggccagggc atgtgtggtg cctcaagtgg ctctattcca gccaatgtag ggcaacctag
      301 tattagggtt aaatctctaa ctgtaggtgg aagaaagggg gataaatagg ttgagatata
      361 gaggactttt aaaccagcta ctttataagg ctaaggatga atttgaggag tctgaagcct
      421 ttttaataga aaataaggaa gtaaatatat ctttatacaa gggagaaata gataagttta
      481 gcatagcaga gtctggtggc ctatccctta ggggtactag agcaggcagg atgggttatg
      541 cctatactga aaaaatagac gaagaggcct tagatattct aattgaggag gcatcagaaa
      601 atagaaagta tattgatgat aaggaaccag aagttatatt tgaaggttcc aagtcctata
      661 agagccaaga taactatagc caggcattgg aggaagtagg gacagaggaa aagataaact
      721 tcttgaaaaa attagaagct gaggctttaa ggcttgataa gagggtattc tctgtaaata
      781 attgtggata tcaagagttt tatcaaaaca ggcatatctt aaacagcaaa ggtgtggact
      841 tagaagatag aacaaatggg gcttttgcct ttatttctgt tgtagtaaag gaaggagaag
      901 atacaaaaac tggcttaagc tataggatag taaaagactt ttcagaactg gattataaaa
      961 aaatggctaa agaggctgta aaaaatgcca gctccatgtt gggagcagaa agtattaaat
     1021 cagataatta tcctatagtg ttcgaaaaca aaacttttgc caacttctta tctgcctttt
     1081 cctctatctt ttccgcagaa aatgtgcaaa aaggtttgtc cggcttaaag gggaaattag
     1141 gtgaagatat tgccaaccct attttaacta ttgtggatga tccattttta aaggatggtt
     1201 ttgcctccaa aaattttgat gatgaaggaa ccagcaccca ggttaccaag ctaatagata
     1261 aaggtaaatt atcatccttg atttacaatt ggaaggctgc taaaaaggat aaggttcagt
     1321 ctactggtaa tgctagcaga tcctataagg ggcagatttc tatttctcca actaacctgt
     1381 atgttcaaaa aggagaaaca gaatttactg gcctaataga tttaaaagaa ggagtattta
     1441 taaatgacct ccaaggatta cactctggat taaatccagt ttcaggtgat ttttccttgt
     1501 ctgctgctgg ttttatgatt gaagatggac aactgggaaa accagtaaac cagataacaa
     1561 ttgcaggaaa cttatatgaa ctactgaagg atatagaagc cataggcaag gatttagaat
     1621 ttagcctgcc aggagccagt tatataggct ctccaagtgt tagggttagc tccctatctg
     1681 tttcaggcaa ataaaaaaag gtgcaagttt taacttgcac cttttctaaa ttagaaagtt
     1741 ttacttaaac tggctatcaa aaggttccaa aacagtaata tggcccatta aagactcagc
     1801 cctttttcca tcaactagaa attgaacctt atttatatta tccagctcca gtaggctggt
     1861 gacaatttga ttgatggtga aggtttcctg caaggaacct ccatgtagtc cttccctggc
     1921 aaagtttaca taagctgtat catctgccac ttcaacttcc aatagcttta cagttgttgg
     1981 tatagaggaa cccaattttg gattttgact acctttcatc aattctctaa ctactacctc
     2041 tgccaggcta atatctccat atttaactag ccttttttcc gggattaaat gttttaaatc
     2101 ttcattacca gtttcaatat attccatatt ggcaaaataa agattaactt ccttagattt
     2161 aatctcgggg gttggatcca ctattatttc tgcttcttcg gccaccgatt tgctattact
     2221 gttagatata ggctgttctg catcactaac aggtgtattc aggtcatctg tattcttatt
     2281 actatcgtct ctttgctcat ctacctgtgg catttgactg tcggtaattt ccccttcatt
     2341 ggatttatct tctagtattt ctgggtatcc atcttgattg ttactaggac tgtcaccctg
     2401 caggcttgat tggctttcgc accctgttat ggcaactatt aaggtaaata ttattattaa
     2461 ggaaaatatc tttttcattt ttactctcct ttcatgtaag tatattttat gcaagaatgt
     2521 ttaaataaga gtaaagagtt gtgaagaaat tattaacaaa gagaaaataa aaagtaaaat
     2581 tacttgggta ttgtgattat aaactggcta ccctgtccca ggctactttc tacatggatt
     2641 ttccactgat gtttgtcaac aattttttta actattgcaa gcccaattcc ctgtccttcc
     2701 acgtaaggct ctgtgagagc tgaatttatc cttctaaatt ctcgtttaaa tatggcaggt
     2761 atatcacttt tatctattcc tattccattg tccttaataa caatctgggc tgaatttttt
     2821 tcttctctta ggctaatttg aaccatagga tttttcttat ttttgtcctt atatttgata
     2881 gcattttcta ttaggtttat aaatacctct ttaagcttat ccctatcaca atagacttta
     2941 atatttccat ccagatctaa gcttatatct acttgatttt tgtttgcata aggagttatg
     3001 aatttaacag tttcctcaag taatttaccc agatcctggt aggttaaatt taaattaata
     3061 tcctccagct ttatagactg ggtaaggtgg tttattaaac ttttcattcg tgcagtttca
     3121 ttctttatat cttttaaaaa ctccttataa acccttatat cattctcacc aattaaaaga
     3181 gagtcaataa gagcctctat agaggttaag ggagttctta attcatggga tacgctgttg
     3241 attaataatt tcctattggc ctcaatatca tataggctgg tactcatctt attaaaggta
     3301 tctataagct ctcccacttc atccttagat ttatgctcga ctttatagcc taagctacca
     3361 ccagatatgg cactaactcc ctttgataat tctcttaagg atttggtcaa ggtatttgtg
     3421 gtaatagcta ttagaaagat agctataaaa agagctattg aggatacttt tataatatct
     3481 tttcttaaat tagaaatatt ttcccataga aaggaaatgg atttggaaat caaaacaact
     3541 ccccttattt gttcttgacc ttgatggttg attctaatgg gaacagtggc ctgtaggatc
     3601 tttttttcat caatattata aatatctgat cctggatgat tatttaagct actttctatt
     3661 tctttattgt taataaactg tccgtaaaga tcattattgc tgtctattag gacagtttta
     3721 ttatcattga gaactaaaat ccttgcatct atccttttgg catagtccct aatcatatta
     3781 tttaaataaa ttatatcatc catattctcc ctatatagat ctgccacaat attagcagtt
     3841 tgaaaaagcc tggtttcctc acttttaatg gaggtggttt tatagttgtt taatatattg
     3901 atgctaaaga ataaaaaggc caaaataatt attaggctaa aaataatcag tgatttatat
     3961 cttattgaaa tagtacccta ccccccattt ggtcataatg tattcaggtt cttttacatt
     4021 tctctcaatt ttttctctga tatttttgat atgtacatct atggttctta catctaaagc
     4081 ttcttctccc cagattaggc taaaaatttc ttctcttgta tagacccggc cagggttttt
     4141 ggccaaaaga tgtaacatat caaattcctt ggcagttaaa acaacctccc ggtccttgat
     4201 agtaactatc ctttcttcta aatttataac catatctcct tgtttaataa ggccagcaac
     4261 ttttctaacc ttactttcca cccttctaga aatagcctta attctggcta tcagctctaa
     4321 ggtgttaaag ggtttggtca tataatcatc agccccaaat tcaaagccta atattttatc
     4381 ataatcccca tctttggctg atagcattaa gataggcata tcataatctt ccctgatttt
     4441 tttacataac atcattccat caatatctgg caacattaaa tccagtataa taaaatctat
     4501 ttcttcattt ttcactgtat ttatagcttg atgtccatca taggctacaa ataccttata
     4561 attttgttgt tccagacttc ttttaagtcc tttagcaatt agtttctcat cttctacgat
     4621 taaaatatta ataccaatca ccacctttta ttattactta tataataaca ccagcttaaa
     4681 aaaataacaa tttataatta aacatctggt aaggtgatgt atgtttgata tataatatgt
     4741 ataacagaaa ggaagggcaa gcatatgttt atcaagattc agttagactc tgatctacct
     4801 atttataaac aattaaaaga caactaatta taggaatagc aaaggaacaa ctcagtccca
     4861 ggggctgcct tctgtaagac agttaagtgg agatttaggt ataaaccttc acactgtaaa
     4921 taaggcctat aatatattaa aggatggagg ataattggtt ataaatagaa gccagggtgc
     4981 tatggaacaa aagggtaaaa agattaaagg aggaaagacg agtacacatc catatttgtt
     5041 ggaaaacggg taggaattgg ctggactgtc aatccaagaa gacctttaga aatgatttta
     5101 ataatcattt ccttattgat cataattatt acaatttttt taattaaata ggaggtgcta
     5161 tatgttaaag ataaaaaatg tgtccaaaag ttatgccggt ggaacaataa aggcagtgga
     5221 taatattagc ttggaagtaa aagcaggaga gatttttggt tttttgggac ctaatggggc
     5281 aggcaaaacc accactataa aaatgattgt agggctttta aaacctgatg aagggactat
     5341 tatgataaag gatcttgatg tatgggataa gccccttaag gctaaatcaa aaatatctta
     5401 tgttccagat aatccagcag tttatgacaa gttaaagggt atagaatatt taaatttcat
     5461 agcagatatg tatgaaatat ctaaggagga aagaaaagac aagatagaaa gatatactaa
     5521 actctttaga atagaagatg ccctaaacga tatgataggc agttactccc aagggatgaa
     5581 acaaaagtta gttttaactt cagccctact gaacaatcca gatttattta tattagatga
     5641 acctatggta ggtttagatc ctaagtcgtc ttttaactta aaggaaatta tgaaggccat
     5701 gtgtgaagaa ggaaggactg tatttttttc cacccatgtt ttggaggtgg cagagcagtt
     5761 atgtgatagg atagccatta taaataaggg aaagattata accactggaa gtatggagga
     5821 attacgggca gatgccaaag aaagagaatc tttggagaaa atattcttgg agttgactga
     5881 ataatggaga aattattatc tttaataaaa atagatctgc ttaacacctt tggcctgtct
     5941 agcctggtcc atagtatgaa agacagaagg acccgttggc aatttatcat attggctata
     6001 gcctttttat cattaatacc cagctatgta atgtttgtaa agggattatc caacatttat
     6061 gttgcctatg accagttagg tcaaagatca tactttttac aactggggat ttttttagct
     6121 cagatggcag tatttttgtt tggaatccta tatgtaatga gtaaatatta ctttgccaat
//
LOCUS       DATGSI010000494         1102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_602879, whole genome shotgun sequence.
ACCESSION   DATGSI010000494 DATGSI010000000
VERSION     DATGSI010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602879"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VK031_07660"
     CDS             complement(<1..456)
                     /locus_tag="VK031_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HLS53834.1"
                     /translation="MIIIKTEEQIEKMKKAGEILANIHKEIGKMIKPGMTTMEIDSFV
                     EDYLKEVGALPEQKGYQGYPYATCASINDEICHGFPRKEPIKDGDIVTIDMVVNLDGW
                     LADSAWSYGVGELSETAEQLLKVTKRSLYLGIEQAVIGNRLGDIGHAIQS"
     gene            complement(479..>1102)
                     /locus_tag="VK031_07665"
     CDS             complement(479..>1102)
                     /locus_tag="VK031_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLS53835.1"
                     /translation="IEKDNKYDRAYYYAANIHDRLGQRDKAINLYKRVIELNPNDFYA
                     YNNLGSLYEELGKYEVAKKYLKGSLDLNPNFYKALFNMGVVKSRLKDNERALAYYRKA
                     IETNKKYYYTYLNMAVIYIGEDKYKNAVEILTEGIRNNPSKEDLYYKRASCFTKLGLE
                     DRAIEDIRQVLSLNLDYLDLIKKDKDFYRLSKVKKFKRILKEYNHNK"
BASE COUNT          297 a          236 c          137 g          432 t
ORIGIN      
        1 ggattggata gcatggccta tatcccctag cctgtttcca attactgcct gctctatacc
       61 aagatacaag gatcttttag ttacctttaa aagttgctct gcagtttcag aaagttctcc
      121 tactccataa ctccaagcag aatcagccaa ccaaccatct aagtttacca ccatatctat
      181 ggttactata tctccatcct ttataggttc ctttctagga aagccatgac agatttcatc
      241 attaatggaa gcacaagtgg cataagggta gccttggtat cctttttgtt ctggtaatgc
      301 tcccacttct tttaggtagt cttctacaaa actatctatt tccatggtgg tcataccagg
      361 ctttatcatc ttcccaatct ctttatgtat attggccagg atttctccag cctttttcat
      421 cttctcaatc tgttcttctg tttttattat tatcataagg tccttccttt cttaaagatt
      481 acttattatg attatattcc tttaaaattc ttttaaactt cttaacttta cttaacctat
      541 agaaatcctt atcttttttt attaaatcta aatagtctaa atttaagctt aatacctgtc
      601 ttatatcctc tatggcccta tcttccaagc ctagcttagt aaaacaagag gccctcttgt
      661 agtataggtc ttctttgcta ggattgttcc taattccctc tgttaagatt tccactgcat
      721 ttttgtattt gtcctctcct atataaatta cagccatatt taaataagta tagtaatatt
      781 ttttatttgt ctctatggcc ttcctataat aggccaaggc cctttcatta tcctttaacc
      841 tgctctttac tactcccata ttaaataggg ccttgtaaaa gtttggattt aaatctaaag
      901 atccttttaa atatttttta gcaacttcat atttacctag ctcttcatat aaagagccta
      961 gattattata ggcataaaaa tcatttgggt taagttctat taccctttta tacagattta
     1021 tagccttatc cctttgtcct aacctatcat ggatgttggc agcataataa taggccctat
     1081 catacttatt atctttttca at
//
LOCUS       DATGSI010000495         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_131314, whole genome shotgun sequence.
ACCESSION   DATGSI010000495 DATGSI010000000
VERSION     DATGSI010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131314"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VK031_07670"
     CDS             complement(<1..428)
                     /locus_tag="VK031_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HLS53836.1"
                     /translation="MLLKVLTEASGVSGNEKEVRDIIISEIKDHVDHIKIDRLGNLIA
                     FKAGKKTDKTLMVTAHMDEVGLLVTDIDSSGLLKFITVGGIDKRILVSKPVTIGKNKI
                     KGVIGAKPIHLQKRNEWTNPLEIDDLYIDIGAKSKADAEK"
     gene            complement(419..1474)
                     /locus_tag="VK031_07675"
     CDS             complement(419..1474)
                     /locus_tag="VK031_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HLS53837.1"
                     /translation="METKLFLESICSNSGISGFEHLLADSIKQYFSKYTDQVTVDKMG
                     NVIALKKGKSGQDNIKVMIAAHMDEIGLMVTDIEDNGFLRFTNVGGIDPRTIVGQEVI
                     VHGSEDLFGVIGSKPPHLQDRDEQSKAIKMKDMTIDIGYPKEKVKELVQVGDPISIKR
                     DLMSLANNRISGKALDDRVGIGSLFKCMKELANYKHDADIFFVATVQEEVSMAGALTS
                     AYNINPDIGIAVDVGFGRTPELNKSNSIELGKGPGITLGGNIHPGLRKELVSIADKYK
                     VPYQMEITPGPTGTDARAMQITREGIPCLVISIPLRYMHTSVEVVDMDDIWSTGKLLA
                     FFIKSLSNDRLEGLLCY"
     gene            complement(1581..>2613)
                     /gene="rpsA"
                     /locus_tag="VK031_07680"
     CDS             complement(1581..>2613)
                     /gene="rpsA"
                     /locus_tag="VK031_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HLS53838.1"
                     /translation="SDSSLSPKELFQEGDEIDVYVLKVDDGEGNVVLSTKRVEEVKNW
                     DEVEEIFENQEIVEVKVLRVVKGGLIALINNINGFIPASHISVDFITDLEQFKDKTLK
                     VRVIDFNRQKRRVILSRRVILSRKVVEEEELEKKREELWNSLEVGSLVEGTVQRLTDF
                     GAFVDLGGVDGLIHISDLSWYRVKHPSEVVQAGDKVEVKVLDFSQERNRISLGLKQTV
                     KEPWVVFLENANVGDVVEGKVVNLLDFGAFIRLKEGVDGLLHVSQISREHVDKPSDVL
                     NIGDQVTVEIIDINEEDRRISLSKRALEEEEEPKEVEEKNDQPKEDESDDFSNEEEYV
                     TIEDVINSK"
BASE COUNT          748 a          606 c          343 g          916 t
ORIGIN      
        1 aacttttctg catctgcctt agatttggca ccaatatcta tatataaatc atctatttct
       61 aaaggattag tccattcatt tctcttttgc aaatgaatag gcttagcccc aataacaccc
      121 ttaatcttat tttttcctat ggtaactggt ttagatacca agatcctttt atcgataccg
      181 cctactgtaa taaactttaa gaggcctgag gaatcaatat ctgtaaccaa taaacctacc
      241 tcatccatat gggcagtgac cattaatgtc ttatctgttt tctttcccgc cttaaaggct
      301 ataagattgc ctagcctatc gatttttata tggtcaacat ggtccttaat ttcagatata
      361 ataatatctc taacctcttt ttcatttcca gaaactccgg atgcttcagt aagaaccttt
      421 aataacataa taacccctcc agcctatcat tggataatga tttaataaag aacgctaaaa
      481 gtttaccagt agaccatata tcatccatat caactacctc tacagatgta tgcatatacc
      541 ttaagggaat tgagatgact aagcatggta taccttctct agtaatttgc atagcacgtg
      601 cgtctgtccc agtagggcca ggagttattt ccatttggta agggacctta tatttatcgg
      661 cgattgatac tagttccttc cttagcccag gatgaatgtt tccacccagg gtaattccag
      721 gtcctttacc taattcaata gaatttgatt tattaagctc tggagtcctt ccaaagccta
      781 catctacagc tatcccaata tctggattaa tattataggc agaagttagg gcaccagcca
      841 tactgacctc ttcttgcact gtagctacaa aaaaaatatc tgcatcatgt ttataatttg
      901 ccaattcctt catacattta aataaggacc caattccaac cctatcatct aaggctttgc
      961 cactgatacg gttattggct aaactcataa gatccctttt tatagatata ggatcgccta
     1021 cttggactaa ttccttaacc ttctctttcg gatagccaat atctatggtc atatctttca
     1081 ttttaattgc cttactttgc tcatccctat cctgtaggtg aggaggttta gagcctatta
     1141 caccaaatag gtcttcgctt ccatggacta taacctcctg gcctacaatc gttctaggat
     1201 caattcctcc tacattggta aatcttaaaa agccattatc ttcaatatca gtaaccataa
     1261 gtcctatttc gtccatatgg gctgcaatca taacctttat attgtcttgg cccgattttc
     1321 ccttctttag ggcaattaca tttcccattt tatccacagt aacctggtct gtatatttag
     1381 aaaaatattg ttttatactg tctgccagta agtgttcaaa gcctgatata ccactgttgc
     1441 tacaaataga ttctaaaaat aatttcgtct ccaaattgaa gcctcctttt aatcacaaaa
     1501 attgattaat taaataaata gtactatagc tattatgata atcacatagc aaatagtact
     1561 attattaatt aattaaattt ttacttacta ttaattacat cttcaatggt aacatattct
     1621 tcttcattgg agaaatcatc tgactcatct tctttaggtt ggtcattttt ttcctctact
     1681 tcttttggct cttcctcttc ttctagagcc cttttactaa ggctgatcct cctatcttct
     1741 tcgtttatat cgattatttc aactgtaacc tggtctccta tatttaagac atcagaaggt
     1801 ttatcaacat gttccctaga aatttgtgaa acatgtaata atccgtctac gccttccttc
     1861 agcctaataa aagcaccaaa atccagtaaa tttactactt ttccttcaac cacatctcct
     1921 acattagcat tctcaagaaa tactacccaa ggctccttaa cagtttgttt taagccaagc
     1981 gaaattctat ttctttcctg gctgaaatcc aataccttaa cttctacctt gtctcctgct
     2041 tgaactacct cagatggatg cttaacccta taccaagata ggtctgaaat atggattaaa
     2101 ccgtctacac caccaagatc tacaaaggct ccaaagtcag tcaacctttg aacagttccc
     2161 tccacaaggc tgcctacttc taggctattc cataattctt ctcttttctt ttctaattct
     2221 tcttcctcta ctacctttct agacaagatg acccttctag acaagatgac ccttcttttt
     2281 tgcctgttaa agtctataac tcttaccttt agggtcttat ctttaaattg ctccaaatcg
     2341 gttataaagt ccacagatat gtgagaggca ggtataaatc catttatatt atttataagt
     2401 gcaattaggc ctccctttac tactcttaga accttaactt ctacaatttc ctggttttca
     2461 aagatttctt ccacttcatc ccaattttta acttcctcta ctcttttagt agatagtacc
     2521 acattgcctt ccccatcgtc tacttttagg acataaacat ctatttcgtc cccctcctga
     2581 aaaagctcct tagggcttag gctagaatca ctg
//
LOCUS       DATGSI010000496         1180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_177120, whole genome shotgun sequence.
ACCESSION   DATGSI010000496 DATGSI010000000
VERSION     DATGSI010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177120"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VK031_07685"
     CDS             <1..559
                     /locus_tag="VK031_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53839.1"
                     /translation="KRREYWRFKCDCGNIKEVLKYNVTSGRSTNCGCMQYVNRKGNVK
                     HGKRYTRLYTIWCSMKSRCNDSENEYYGGRGITYCDEWEDFEPFYRWAMANGYNDELS
                     IDRIDSNGNYEPSNCRWVDSTTQSINRRVKSTSTTGITGVNETLDNTYVAKITVDGNT
                     IRLGTFKNIEDAIKARKKAEEKYFK"
     gene            632..826
                     /locus_tag="VK031_07690"
     CDS             632..826
                     /locus_tag="VK031_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53840.1"
                     /translation="MSKRLELIDTKDKFSLKIDGNEIPFITEYTITAKAESSIVELGL
                     KLAIPNEKDIIIESEKAKVL"
     gene            865..>1180
                     /locus_tag="VK031_07695"
     CDS             865..>1180
                     /locus_tag="VK031_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53841.1"
                     /translation="MGLKEVIKNMVMKMLNIVPATDNQISIKEPLSYTGTVLRNRIWY
                     RGDPSEIDQFFKQAAIDDVGRSRFWAAVPSADSSIRKFHSGLPGEIVDKLADIVIADL
                     DSI"
BASE COUNT          467 a          142 c          257 g          314 t
ORIGIN      
        1 caaaaggcgt gagtattgga gattcaagtg tgattgtggc aatataaaag aggtgttgaa
       61 atacaacgtt acaagtggta ggtctacaaa ttgtggttgt atgcaatatg ttaatcgaaa
      121 aggaaatgtt aagcatggga aaagatatac aagactatat accatatggt gtagtatgaa
      181 aagtcgatgt aacgattctg aaaatgaata ttatggtgga agaggaataa cttattgtga
      241 tgaatgggaa gattttgaac cgttttatag atgggctatg gcaaatggtt ataatgatga
      301 gctatctata gacagaattg atagtaatgg caactatgag ccttccaatt gtaggtgggt
      361 agatagtaca acacaaagca taaatagaag agttaaatct acaagcacaa caggcataac
      421 aggagttaat gaaaccctag acaatactta tgtagccaaa ataacagtag atggcaatac
      481 cataaggcta ggaacattca aaaatataga ggacgctatt aaagcaagaa aaaaagcaga
      541 agagaaatat tttaaataga gctttaaaaa ggctctattt tttatggctg gattccattt
      601 agaactaaga taggaatggg ggggtaagat tatgagtaaa agattggagc ttatagacac
      661 taaagacaaa ttttccttaa aaatagacgg taatgaaata ccctttatta ctgagtatac
      721 cattaccgct aaagcagaaa gtagtatagt tgagttaggt ttaaaacttg caatacctaa
      781 tgaaaaagac attattattg agtctgaaaa agcaaaagta ctataataat ttccagtaac
      841 tagtattgaa taggaggata aattatggga ttgaaagagg tgataaaaaa catggtaatg
      901 aaaatgttaa atatagtacc tgcaacagat aatcaaatat caattaaaga accactatca
      961 tatacaggga cagtattaag aaatagaata tggtacagag gagacccttc agagatagac
     1021 cagttcttca agcaggctgc tatagatgat gtaggtagaa gtagattttg ggcagcagta
     1081 ccttcggctg attcaagcat aaggaaattt cacagtggac tacccggtga aatagtagat
     1141 aaattagccg atattgtaat tgctgacctg gatagcataa
//
LOCUS       DATGSI010000497         1823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_457737, whole genome shotgun sequence.
ACCESSION   DATGSI010000497 DATGSI010000000
VERSION     DATGSI010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457737"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VK031_07700"
     CDS             complement(<1..682)
                     /locus_tag="VK031_07700"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HLS53842.1"
                     /translation="MEIIEIIKAIILGIVEGITEWLPISSTGHMILVDEFMQLSISAE
                     FKEMFFVVIQLGAIMAVVLLYFNKLNPFSPRKTAREKRDTWVLWFKVVVAVIPAGVIG
                     VLYDDIINDMFYNYVTVAITLIIYGVLFILVENRNKGKRPKINDLSELTYKTAFLIGV
                     FQVLSLIPGTSRSGATIVGAMLVGTSRFVATEFTFFLAIPVMFGASLLKLLKFGLVFT
                     SSELVILSV"
     gene            973..1386
                     /locus_tag="VK031_07705"
     CDS             973..1386
                     /locus_tag="VK031_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HLS53843.1"
                     /translation="MEISAKELRKKLEENNIRPSFQRIKIFQYLLNNRCHPTVEMIYD
                     ELHKEMPTLSKTTVYNTLNLFDKANLVRVLSFDGVESRYDINTKDHGHFICESCSNIY
                     DFPIDINSLRFSQLEDFEIKEKNIYFKGLCNKCIN"
     gene            1533..>1823
                     /locus_tag="VK031_07710"
     CDS             1533..>1823
                     /locus_tag="VK031_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004834207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HLS53844.1"
                     /translation="MKKRVILGMSGGVDSSVSALLLKEAGYEVIALFMKNWDEKFDDG
                     ACTATEDANDARNVASQLGIPFYTINFEKEYWDRVFTYFLDEYKKGRTPNPDV"
BASE COUNT          661 a          286 c          283 g          593 t
ORIGIN      
        1 caactgataa aattaccaat tctgaactag taaatacaag gccaaacttt aaaagcttta
       61 aaagactggc tccaaacatt acaggaattg ctaagaaaaa ggtaaattca gtggcaacaa
      121 acctggaagt tcccactaac atggccccaa caattgtagc ccctgatcta gatgtgccag
      181 gtattaagga taatacctgg aatacaccta ttaaaaaagc agttttatat gtaagttctg
      241 acaaatcatt aatcttaggt cttttgccct tgtttctgtt ttcaacaaga ataaataaga
      301 ctccgtaaat tattagggtt atggcaacag ttacgtagtt gtagaacatg tcatttatta
      361 tatcatcata aagcacccca attacccctg cagggataac tgctaccact accttaaacc
      421 aaagtaccca ggtatccctc ttttccctag cggtttttct aggtgaaaaa ggatttaatt
      481 tgttaaaata caacaatact acagccataa ttgcacctaa ttgtatgact acaaaaaaca
      541 tttccttaaa ctctgctgat atacttaact gcataaattc atcaactaga atcatatgtc
      601 ctgtactact gataggcaac cattctgtta taccttcaac aatacccagg attatagcct
      661 taattatttc aataatttcc atatcttttt ctcctttacc taaaataata ttactttccc
      721 tatattatca agataatatt agataatcaa gtataaagaa tagggattgg tcctcattat
      781 taaattttct ttggaagatg atatagacta attatatcct aatatatgaa acttttacaa
      841 tatgtcttta atttttctat agagagtttt ttaatgcagc aattgacagg agaagtaaat
      901 taaattataa taattgtaat aattacaaga aagtttaatt aataagtaac catagtaaag
      961 aaaggaattt ccatggaaat atctgcaaag gaattaagaa aaaaattaga agaaaataat
     1021 ataaggcctt cctttcaaag gataaaaatc ttccagtacc tattaaataa taggtgtcac
     1081 cctactgtgg aaatgattta tgatgaactt cacaaggaaa tgcctacttt atcaaaaact
     1141 accgtatata acactcttaa tttatttgat aaggcaaatc tagtccgggt gttatccttt
     1201 gatggtgtgg aatctagata tgatattaat actaaggatc atggccattt tatctgtgaa
     1261 tcttgtagta atatttatga ctttccaata gatattaata gtttaagatt tagtcaactg
     1321 gaagattttg aaatcaagga gaaaaacatc tactttaaag gcctgtgtaa taaatgtata
     1381 aattaaaaga ttgattgtct tgtcagatat tccttcattt atgaaaatat ctgacatttt
     1441 ttatgtagtt agtttgtata tggactacaa ttatatgata atatatttaa taactagaat
     1501 aatagatgca actaatagaa aaggtgaact atatgaaaaa acgagttatt ttaggtatga
     1561 gtggtggtgt agactcctct gtatctgcac ttctacttaa ggaggctggc tacgaagtaa
     1621 ttgccctatt tatgaaaaat tgggatgaaa agtttgatga cggtgcctgt acagccactg
     1681 aagatgccaa cgatgcaaga aatgtggctt cccaattagg catacctttc tatacgataa
     1741 attttgaaaa ggaatactgg gatcgagttt ttacctattt tctagatgag tataaaaaag
     1801 gtaggacgcc aaatccagat gtt
//
LOCUS       DATGSI010000498         1133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_131487, whole genome shotgun sequence.
ACCESSION   DATGSI010000498 DATGSI010000000
VERSION     DATGSI010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131487"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VK031_07715"
     CDS             <1..846
                     /locus_tag="VK031_07715"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HLS53845.1"
                     /translation="GLTQALLDAAAKAKKVTMAEVIKEEYDTGIDLKRIPIFTQSGDD
                     RYINADKMIIKEADVMPHGLFNNVEEKLGLKGEKLIEYVEWLRDRVLKYRVNEDYNPV
                     FHIDVYGTIGELLDDDIEKMTEYLGRLEEVAKPFLLRIEGPMDVGSREAQMEAMRDLR
                     ASLEKEGITVELVADEWCNTLEDVKYFAENKAGHMIQVKSPDLGGINNSIEALLFCNE
                     IGIGAYLGGTCNETSRSAEICANIAIACGATQVLAKPGMGVDEGIMIINNEMNRVLAL
                     AKKRA"
     gene            894..>1133
                     /locus_tag="VK031_07720"
     CDS             894..>1133
                     /locus_tag="VK031_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53846.1"
                     /translation="MLQFRNNRFKDLINKKRWYSMTGTSASVKIREIEASQITEEVKR
                     LCVEANYFLPEDVVERLEKMKEEDGWDLSKATIDKI"
BASE COUNT          419 a          163 c          283 g          268 t
ORIGIN      
        1 ggcctaactc aagccctact ggatgcagca gccaaggcca agaaagtaac catggctgag
       61 gttatcaagg aagaatatga tacaggtatt gacttaaaga gaatccctat atttacccaa
      121 tctggtgatg ataggtatat aaatgctgac aagatgataa taaaagaagc agacgtaatg
      181 ccacatggtc tatttaacaa tgtggaggaa aagttaggcc tgaaaggtga aaaactaata
      241 gagtatgtag agtggctaag agatagggta ctgaaatata gggttaatga agactacaat
      301 ccagtattcc atatagacgt atatggtact attggagaat tgctagatga tgatatagaa
      361 aagatgaccg agtacttagg caggctagaa gaggtggcta aacctttcct actgagaatc
      421 gaaggaccta tggatgtagg tagcagagag gctcaaatgg aggccatgag agatctaagg
      481 gctagtttag aaaaagaggg catcactgta gagttggtgg cagacgaatg gtgcaataca
      541 ttggaagatg taaaatattt tgctgaaaat aaggcaggcc acatgattca ggttaaatca
      601 cctgacctag gtggaataaa caactccatc gaagccctac tattctgtaa tgaaataggc
      661 ataggagctt acctaggtgg aacttgtaat gaaaccagca gaagtgctga aatctgtgcc
      721 aatatagcca tagcctgtgg tgcaacccaa gtattggcta agccaggcat gggagtagat
      781 gaaggaataa tgataataaa caacgaaatg aatagagttt tagctttggc taagaaaaga
      841 gcatagtcca ttagactgtg taaaactgaa tacgccacct cctaggaggt ggtgtgttac
      901 agtttagaaa taataggttt aaagacttaa taaataaaaa gaggtggtat tcaatgacag
      961 gaaccagtgc aagtgtaaaa attagagaga ttgaagccag tcaaattaca gaagaggtaa
     1021 aaaggctttg tgtagaggct aattactttt taccagaaga tgtggtagaa agactagaaa
     1081 aaatgaagga agaagacggt tgggacttat ctaaggccac aattgataaa atc
//
LOCUS       DATGSI010000499         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_315578, whole genome shotgun sequence.
ACCESSION   DATGSI010000499 DATGSI010000000
VERSION     DATGSI010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315578"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..949
                     /locus_tag="VK031_07725"
     CDS             119..949
                     /locus_tag="VK031_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53847.1"
                     /translation="MDGVQILKTSLPADPYFTMTGIGLPKVSSWIIASVLGACTAQAG
                     IQPILAAKDVDVAKKSALLTALIVAPFGIFTAVLGMIARVKYPGLENAKLALPTLMMG
                     LPPVAGGIVLASMMAAILSTVSPIILAAGTMATKDIYQKLINKEATDGELLRVSRLTT
                     GVSGVICTLVALVMYGSTKVLDIVYFAYTLRGAIFVVLLLGIYWKRTTSKGAIYSMVA
                     TAVVGFVWVLYYSRTGHYPIHDGFTETYASVLVAFISTIVFSIIYKDDFNTQKLEYQK
                     "
     gene            1056..1928
                     /locus_tag="VK031_07730"
     CDS             1056..1928
                     /locus_tag="VK031_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLS53848.1"
                     /translation="MKDRLVGTLSIVIAAVIFGIMPLFAKIIFSNGGDPVSLVFYRFF
                     VPIPVLYFINKKRQIDLTISWKEVLRLIVLSLFGISASLILLYYSYNYISIGLATAIF
                     YIYPIFVAIGCVLFFEHKMTPGEVLSVILCGLGMFLFLDNIGKVNIIGILLSFLSGIC
                     YAFYIVYFDKSSLRTMDIYKTTYYLCIVSSGLLFIYSLAMDTLVIKLSPLCWFLLIFV
                     SLIVTIVAVTLFQYGIKLIGSQKASIMGTFEPITSVILGVSILEESFNVRTLLGFVII
                     LTSVVLSQYSGKVT"
BASE COUNT          619 a          271 c          388 g          747 t
ORIGIN      
        1 gaaggaaaag cgatccaagc tctcgataac tggtttgaat cggatgagag tctctcggac
       61 ctgcagcaga aatgggacga aattttcgaa tcgtgccata ttactaaccc ttggagatat
      121 ggatggtgtt caaattttaa agacttcatt accggctgat ccatatttta ctatgacggg
      181 cattggttta cccaaggtat catcttggat aatagcttct gttttgggag catgtacagc
      241 tcaggcaggt atccagccta tattagctgc caaagatgtt gatgttgcta agaaatcagc
      301 ccttcttact gcattaatag tggcaccttt tggtatattt actgctgttt taggcatgat
      361 agctagagtt aagtatcctg gtttagaaaa tgccaaactg gcattaccta cattaatgat
      421 gggattgcca ccggtggcag gaggtatagt tctagcttct atgatggctg cgatactttc
      481 tacagtatca cctattattt tagctgctgg aactatggct acaaaggata tatatcaaaa
      541 acttataaat aaggaagcca cagatgggga actgcttagg gtgtcaaggt taactactgg
      601 agtatctggt gttatctgta cattagtagc attagttatg tatggtagta ctaaggtatt
      661 ggatatagta tactttgctt acactttaag aggggccata tttgtggtat tactattagg
      721 catctattgg aaaagaacca catctaaggg agctatatat tcaatggtag ctactgcagt
      781 tgttgggttt gtttgggttt tatactattc tagaactggc cattatccta tacatgatgg
      841 ttttacagaa acttatgctt ccgttttggt tgcttttatt agtacaattg tttttagtat
      901 tatatataaa gatgacttta atactcagaa attagagtat cagaaataac ttatatttaa
      961 aatgctcttt tggaggattt ataaaaagaa tccaaaaggg cttttttatg ggaaaatcca
     1021 caaattaaga agatggagaa aggattgatc atataatgaa ggatagattg gtgggtacat
     1081 tatccatagt aattgctgca gtgatttttg gaataatgcc cttattcgct aaaataatat
     1141 ttagtaatgg aggagatcca gttagtctgg tgttttatag attttttgtt cctatacctg
     1201 tactatattt tataaataag aaaaggcaaa tagatcttac tataagttgg aaggaagttt
     1261 taagattaat agtactttca ttatttggta tatcagcttc attaatttta ttgtattatt
     1321 cctataatta tatatcaata ggactagcca cagctatatt ttatatatat ccaatatttg
     1381 tggccatagg atgtgtcttg ttttttgaac acaaaatgac tccaggagag gtattatcgg
     1441 ttatactatg tggactaggc atgtttttat ttttagataa tattgggaaa gttaatatta
     1501 taggtatttt attatctttt ttatcgggaa tttgttatgc gttttatata gtttattttg
     1561 ataaaagttc tttaaggacc atggatattt ataagactac atattattta tgcattgtat
     1621 catctggatt actatttata tatagcttgg ccatggatac attagtaatc aaattaagcc
     1681 ctttatgttg gttcttacta atatttgttt ctttaatagt aacaatagtg gcagtaacat
     1741 tattccaata tggcataaaa ttaataggtt ctcaaaaagc ttctatcatg ggtacattcg
     1801 aacctattac aagtgttata ttaggtgtgt ctatattaga ggaaagtttt aatgtaagga
     1861 cattactagg atttgtgatt attttaacat cagttgtatt atcacaatat tctgggaagg
     1921 ttacttgaga tgttgtaata aatatgaaat aacccttcta ctttgttaaa tagtagataa
     1981 tttcttcctt atttgtatat ttagttgcta cttctgctac tatac
//
LOCUS       DATGSI010000500         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_555329, whole genome shotgun sequence.
ACCESSION   DATGSI010000500 DATGSI010000000
VERSION     DATGSI010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555329"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VK031_07735"
     CDS             complement(<1..475)
                     /locus_tag="VK031_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HLS53849.1"
                     /translation="MIYISLLWNFLKIGALAFGGAYGAVPLIQDIILANNWLTEEMFG
                     NLVGISESTPGPIMVNMATYIGNSQAGLPGAILATLGVVLPSFIVILLVTIAFASWIE
                     KRKVQAALKGIKPAIMGIVLATGVSMALKEILGKFDKPSLDIRALLILALLFLVAG"
     gene            complement(476..1048)
                     /locus_tag="VK031_07740"
     CDS             complement(476..1048)
                     /locus_tag="VK031_07740"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HLS53850.1"
                     /translation="MNILLDLFFTFVKIGGFTFGGGYAMIALLDYECVERKGWITKEE
                     LMDVTVIAESTPGPIAINCATYTGYKQAGLAGAVLATLGIVFPSFLIIMIISSFLEDI
                     LTYSLVVKAFKGIRLAVALLIFQAGVKMTRSMMRKTQDKRISICFTASTFVLLLLLNI
                     LAIQLSTIYLIIIAALLGFLIFYLARREEG"
     gene            complement(1360..2469)
                     /locus_tag="VK031_07745"
     CDS             complement(1360..2469)
                     /locus_tag="VK031_07745"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HLS53851.1"
                     /translation="MSQDNYNIKKSKRFNHLNFTERNLIEKWLKEGTSVANIAKRLGR
                     HRSTIYREIKRGTVNQIKNINGYLKNVEVYYADSGQMKYERNREKSKSKGPKKFSKNF
                     FKALTKAKENKQLGGKNRIYNIKSFVHCYKRDNPLDTNIPSFKTVYRYIKKGLIDIKP
                     HDLPVMYRLAPRKNKYSKPKGQNKRILGKSISSRDPKVLKREEFGHWEADLVEGRKGK
                     NEPVILSLVERKTRYGITRKLKNSTADTAYEALKEIIESKKDMFLTITFDNGSEFSRV
                     SELEDDSLDIYFAHAYSAWERGTNENFNKLLRDFVPKGKSIKDYSQEYIIKAGKKIND
                     RIREVIGFKSASEKLQIEIDKKFALPKGETVVSKR"
BASE COUNT          740 a          525 c          401 g          895 t
ORIGIN      
        1 aaccggccac taagaaaagt aaggccaata taagtagggc ccttatatct aaggatggtt
       61 tatcaaattt ccctagaatt tctttcaagg ccatggacac acctgtggcc agaactattc
      121 ccataatggc aggctttatg cccttaaggg ctgcttgtac ctttcttttt tctatccagc
      181 tggcaaaggc tatggtaacc aataaaatta ctataaagga tggcaatact actcctaggg
      241 tggccagaat agcccccggt agacctgcct ggctattgcc tatataggtt gccatattta
      301 ccataatggg ccctggggtg gattcgctaa ttcctaccag attgccaaac atttcttcag
      361 tcagccagtt attggccagg ataatatcct ggataagtgg cacagcccca taggcaccgc
      421 caaaggctag ggcccctatc tttaaaaagt tccataataa agatatataa atcatttagc
      481 cctcctccct tctagccagg taaaagatta aaaatcctaa taaggccgct attataatca
      541 ggtagatggt ggacagctgg atggccaata tatttaaaag taggagcagg acaaaggttg
      601 aagcggtaaa gcatatgctg attcttttat cctgtgtttt tctcatcatg gacctggtca
      661 tctttactcc agcctgaaaa attaataggg ctacagccag acgaatgcct ttaaaggcct
      721 tgacaaccag agaataggtc aaaatatctt ccaggaagct ggagataatc ataataatca
      781 aaaaggaagg aaaaacaatc cctaaagtgg ccaaaacagc tccagccaac cctgcctgct
      841 tatatccagt ataggtggca cagtttatgg ctatagggcc tggagtagat tcggcaatta
      901 ctgtcacatc cattagttct tcttttgtaa tccaaccttt tctttccaca cattcataat
      961 ctaaaagggc aatcatggca tagcctcccc caaaggtaaa gccaccaatc tttacaaagg
     1021 taaaaaataa atccaacaat atattcatga ttccttcccc cccttcctat attatcatgc
     1081 ctattttcaa agccgtcaaa tatttagata ggcagaacaa attaaatctt ttaaggataa
     1141 taaaaaaggc atccaatggt gctttaccat agggatttat aactctaaaa aaattggcat
     1201 agtcggttta gatatcggct atgccgtttt gctttctgta ttaggaatat ctatagcacc
     1261 aatgcagtta tagtatatcc ttattttctg ctgtcttttg ccatttattt tctacttagt
     1321 tgtcaaatca attgcgacag ttttcaaacg gaagttttgc tatcttttag ataccactgt
     1381 ctctccctta gggagagcaa attttttatc tatttctatt tgtaatttct cagatgcact
     1441 tttaaaacct atgacttctc ttattctatc atttattttc ttgcctgctt taattatata
     1501 ctcttgagag taatccttaa ttgatttacc tttaggaaca aagtctctta gcagtttatt
     1561 gaaattttca tttgtacccc gctcccatgc tgaataagca tgggcaaaat atatgtctaa
     1621 gctatcgtct tctagttcac ttaccctaga aaactctgac ccattatcaa atgtaattgt
     1681 taagaacata tcttttttag attcgattat ctcttttaaa gcttcatagg ctgtatcagc
     1741 tgtagaattt tttaacttcc tagttatccc atacctagtc ttacgctcaa ccaatgacag
     1801 tatgactggt tcattctttc ctttcctacc ttctactaga tcagcttccc agtgtccaaa
     1861 ttcttccctt tttaatactt tgggatctct tgaggaaatt gactttccca atatcctttt
     1921 attttgacct ttaggctttg aatacttatt ttttcttggt gccaacctgt acattacagg
     1981 aagatcatga ggcttaatat caattaaacc ttttttaata tatctgtaaa cagttttaaa
     2041 ggaaggaata ttagtatcta aggggttatc tcttttgtag caatgaacga aactttttat
     2101 attatatatt ctattcttcc cacccaattg tttattttct ttagccttgg ttagcgcttt
     2161 gaaaaaattc tttgagaatt tcttaggtcc cttagattta gacttttccc gatttctctc
     2221 atatttcatt tgtccagaat cagcataata tacttctaca ttttttagat atccattaat
     2281 atttttaatt tgattaacgg ttcctctctt aatttcacgg taaatagttg atctatgcct
     2341 accgagcctt ttagctatat tagcaacaga agttccctcc tttaaccact tctctattaa
     2401 gtttctttca gtaaaattta aatgattgaa cctttttgat ttttttatgt tataattgtc
     2461 ttgagacatg aaatttgctc ctttcttggg tgtggtacct taagtatagc aaatttctat
     2521 gtctcttttt tatttgtcgc agttcatttt acaatacacc a
//
LOCUS       DATGSI010000501         1186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_131620, whole genome shotgun sequence.
ACCESSION   DATGSI010000501 DATGSI010000000
VERSION     DATGSI010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131620"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VK031_07750"
     CDS             <1..801
                     /locus_tag="VK031_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HLS53852.1"
                     /translation="PSDYMIERMIREDLLEKLDKENIPNLEKVGQRFLNLDYDPDNQY
                     SVPYMWGTVGILYNKSMISDKLDSWNDLWNEKYKEEIVMLKSQRDTIAVALKKLGYSM
                     NTRNIEELERAKEELIKQKPLVYSYLGDEVRDIMVGEDAAIAVVWSGDAVEMIRENDN
                     LEYVIPKEGTNLWFDAMVIPKGAKNKEGAEKFINFLTRPDIAAKNAEYMGYSSPIPEA
                     VELLSNELKSSQVAYPSEDMLANTEIFKDPKDIVKEYDRIWTDIMAGQ"
     gene            918..>1186
                     /locus_tag="VK031_07755"
     CDS             918..>1186
                     /locus_tag="VK031_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HLS53853.1"
                     /translation="MKYITNNSNDPRYNLAFEEYCFKYLPKDEDYVILWINSPAIIVG
                     KNQNTIEEVNQGYVKKKGISVVRRITGGGAVYHDLGNLNFSFIVNT"
BASE COUNT          459 a          169 c          251 g          307 t
ORIGIN      
        1 ccttcagact acatgataga aagaatgata agagaggatt tattagaaaa actagataaa
       61 gaaaatatcc ctaacctgga aaaagtaggc caaagatttc taaacttaga ctatgatcct
      121 gacaatcaat attctgtacc ttacatgtgg gggactgttg gtattctcta taacaaatct
      181 atgatttcag ataagttaga tagctggaat gatttgtgga atgaaaaata caaggaagaa
      241 atagtcatgc taaaaagtca aagggatact atagctgtag ccttaaaaaa attaggctac
      301 tccatgaaca ctagaaatat agaagagttg gaaagggcca aggaggaatt aatcaaacaa
      361 aaacctttag tatactccta cctaggcgat gaagtaagag atataatggt aggggaagat
      421 gcagccatag cagtggtgtg gtctggagat gcagtagaga tgatcagaga aaatgacaac
      481 ttagagtatg taatcccaaa agaagggacc aacctatggt ttgatgccat ggttatcccc
      541 aaaggggcca aaaacaagga aggggcagaa aagtttataa actttctgac tagaccagat
      601 atagcagcaa aaaatgctga atatatgggt tattcctcac ctattccaga ggcggtagag
      661 cttctgtcaa atgaactgaa aagtagccag gtggcctatc caagtgagga tatgctagca
      721 aatactgaaa tattcaagga tcctaaggat atagtcaagg aatatgatag gatttggact
      781 gatataatgg caggccaata gaaaacttta tatttaccaa aaatattgta taataagggt
      841 agaagcatag acttcctgca taaagatttt tctactgggt aaaaatcaaa tgattattaa
      901 aattggaggt ctaatgtatg aaatatataa caaataattc caatgatcca agatataacc
      961 tggcctttga agagtattgt ttcaaatatt tgccaaaaga cgaggattat gtaatactat
     1021 ggataaacag ccctgccatt attgtaggga aaaatcaaaa taccatagaa gaagtcaacc
     1081 aaggatatgt aaagaaaaaa ggtatcagtg tggtcagaag aataacaggt ggtggagcag
     1141 tctatcatga tctgggtaac ttaaattttt cctttatagt taatac
//
LOCUS       DATGSI010000502         1386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_555364, whole genome shotgun sequence.
ACCESSION   DATGSI010000502 DATGSI010000000
VERSION     DATGSI010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555364"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VK031_07760"
     CDS             complement(<1..878)
                     /locus_tag="VK031_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004822379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLS53854.1"
                     /translation="MKEKTVYICSNCGYETIAYMGKCPGCNSWNSLEETKIVASRGTK
                     QRASSISRKKPIKRLREIELSNSQRIVTSINEFNRVMGGGIIKDSVTILSARPGAGKS
                     TLLLQVANELASMGHKVLYASGEESESQIKSRANRILDKVDANTWVISDNSLDNILDN
                     IDEIDPDFIIIDSIQTFTLEAFPNSRAGSPTQTMECAHQLVKVAKNPDRPRAIFMVGQ
                     MTKEDELAGVRALEHLVDTVLLIEGESGEELRTLLTTKNRYGSTGEMGFFNMTDKGMV
                     SIDNPSEYFTTARDQG"
     gene            complement(912..>1386)
                     /locus_tag="VK031_07765"
     CDS             complement(912..>1386)
                     /locus_tag="VK031_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLS53855.1"
                     /translation="IIMTSNVGATSIRKQNVLGFAASTDQDKEEYERMKETVTEELKK
                     TFRPEFLNRLDEVIVFHSLKEEQVREIVDIMVGDLEKRMAKLDINIKVTENTKEHISK
                     QGFDPVYGARPLERTIRRMIEDQLAEEILRGNLSKEDSIIIEYEDNQLVFKKESL"
BASE COUNT          353 a          343 c          192 g          498 t
ORIGIN      
        1 tttccctggt cccgggcagt agtaaaatat tctgatgggt tgtctatgga aaccattccc
       61 ttatctgtca tattaaaaaa acccatttca ccagtagaac catatctatt tttagtagta
      121 agtaaggttc ttaactcctc cccactttcc ccctcaatca acaaaacagt atctaccaaa
      181 tgctccaagg ccctgactcc tgccaattca tcttccttgg tcatctgtcc taccataaag
      241 atagctctag gtctatcagg atttttagca acctttacca gttgatgggc acactccatt
      301 gtctgggtcg gtgatcctgc cctagaatta ggaaaggcct ccaatgtaaa ggtttgaata
      361 ctatctatta ttataaaatc tggatctatt tcatcaatgt tatctaatat attatccagg
      421 ctattgtctg agattaccca agtattagca tctaccttat ctaggatcct attggcccta
      481 gatttaatct gggattcaga ctcctcgcct gatgcataca gtaccttatg ccccatactg
      541 gccaactcgt tggccacctg taacaataag gtggatttac ctgcacctgg cctagctgaa
      601 aggatagtaa ctgaatcctt tattattcct ccacccataa cccggttaaa ttcattaata
      661 cttgttacaa tcctttggct gttacttagc tctatttccc tcagcctctt tataggcttt
      721 ttcctactaa tagaacttgc cctttgtttt gtccccctac tggctacaat tttcgtctcc
      781 tccaagctat tccagctatt acaaccagga cacttaccca tataagctat agtttcgtaa
      841 ccacaattgc tacatatata tacagttttt tctttcatat ttttcccctc ttgtttaaag
      901 agagcctgta tctacaggct ctccttttta aatactaatt gattatcttc atattctatt
      961 ataatgctgt cctctttact tagattaccc cttaggattt cctctgccaa ctggtcttct
     1021 atcatccttc taatggtcct ttctaaaggt ctagcaccat atactggatc gaagccctgc
     1081 ttactaatat gttccttagt attttctgtt acttttatat ttatgtcaag tttagccatt
     1141 ctcttctcta aatctcctac cataatgtct acaatttctc ttacctgctc ctctttcaag
     1201 gagtggaaca ctataacttc atccaatcta tttaaaaatt ctggcctaaa ggtctttttc
     1261 aattcttcgg ttacagtttc cttcatccgc tcatattctt ccttgtcttg atctgttgaa
     1321 gctgcaaaac caaggacatt ttgctttcta attgaggttg cccccacatt agatgtcatg
     1381 ataatt
//
LOCUS       DATGSI010000503         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_177422, whole genome shotgun sequence.
ACCESSION   DATGSI010000503 DATGSI010000000
VERSION     DATGSI010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177422"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VK031_07770"
     CDS             <1..283
                     /locus_tag="VK031_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53856.1"
                     /translation="DDIRRVKLDSTFGSMKVYFENITMKDNHLLVDVDATFTGVELYI
                     PREWDVINDADVTFGSVNEKNKNISTGAGTNTLLLKGDITFASVDIFYI"
     gene            394..861
                     /locus_tag="VK031_07775"
     CDS             394..861
                     /locus_tag="VK031_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HLS53857.1"
                     /translation="MTEEELLAKVKKEAEDYYRRGEFFCSESVVYTINNLLGKPYDDN
                     IIRMASGFPMGMGKAGCLCGAVSGGQMALGMIYGRVRGQCMDKKMIEISKELHDYVKK
                     EYGSNCCRIITRQWRGDDFQSPERKKHCVEITGRIAQWVAKKLIEDGQINLEK"
BASE COUNT          351 a          127 c          243 g          314 t
ORIGIN      
        1 agacgatata agaagggtaa agttagatag cacctttgga tccatgaagg tttattttga
       61 aaatattaca atgaaggata atcatttgct ggtagatgta gatgctacct ttaccggggt
      121 agagttgtat atacctaggg agtgggatgt gattaatgat gccgatgtaa cttttggatc
      181 tgtaaatgag aaaaataaaa atattagcac tggagctggt acaaataccc tcttattaaa
      241 aggagatata acctttgctt cagtagatat tttctatatt taaaatagga gaatttaaat
      301 acctggtatt ttagatactg ggtatttttc tgtttaattg tatatttttt tggtaaataa
      361 aaaggagagt ctatatatac ttaggaggtt attatgacag aagaggaatt attagctaaa
      421 gtaaaaaagg aggcagaaga ctattataga agaggtgagt ttttctgctc cgaatctgtg
      481 gtatacacca taaacaatct attaggtaag ccctatgatg acaatataat aaggatggcc
      541 tcaggctttc ccatgggtat gggtaaggca ggatgtttat gtggagctgt aagcggtgga
      601 caaatggctt taggtatgat ctatggcagg gtccgtggcc agtgtatgga taagaaaatg
      661 attgaaatat ctaaagaact acatgattat gtaaaaaagg aatatgggtc caattgctgc
      721 aggattataa ccaggcagtg gaggggcgat gattttcaaa gcccggaaag aaagaagcac
      781 tgtgtagaaa ttactggtag gatagcccaa tgggtggcta agaaactaat agaagatgga
      841 cagattaacc tagaaaaata aactctcctt ccctaagata ttccttgggg aaggagagtt
      901 ttatttaata ggtggaatct aaaaattcta tggtcctatt taataagtct tcggtacata
      961 ggctgtcttc atttctatca aagtcatgct catcaagatg aaaggttagt aggctagagt
     1021 ttttgattat agaag
//
LOCUS       DATGSI010000504         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_555498, whole genome shotgun sequence.
ACCESSION   DATGSI010000504 DATGSI010000000
VERSION     DATGSI010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555498"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VK031_07780"
     CDS             complement(<1..793)
                     /locus_tag="VK031_07780"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HLS53858.1"
                     /translation="MKKINLALVGATGLVGGTILKLLEERQFPVGQLYLFSSKRSAGE
                     RLKFKGREYVVEELKSDSFDRDIDIALFAAGGAVSERFAPLAAERGVRVIDNSSVFRM
                     KEGIPLVVPEINPEDIKGENIIANPNCSTTQSVIPLKALYDKYGIKRIIFSTYQAVSG
                     SGLGGLRDLEKGKVEFYPYQIQHNALPHIDSFLDNGYTKEEMKMIEETKKILKDDKIG
                     ITATAVRIPVKYGHSVSINVELKKSFKIEDIKKELRNFPGIKLYDN"
     gene            complement(807..2120)
                     /locus_tag="VK031_07785"
     CDS             complement(807..2120)
                     /locus_tag="VK031_07785"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HLS53859.1"
                     /translation="MEIIVSKFGGSSLADARQFKKVKEIIDQDPRRKFIIPSAPGKRH
                     GRDHKITDLFYMCYQLASHGLNFDEVFNIIRERFLSICQGLKIDLNMDNILEEIKAKI
                     EKGSSKDYCGSRGEYLNGLILSKYLNFHFLDPKDFILFTDNGELDQKATEHRLRERVK
                     NIDRAVIPGFYGARKDGQIKTFSRGGSDVTGSIVANGLGAILYENWTDVSGFLMADPN
                     IVKEPNPIEEISYKELRELSYMGAPVLHEEALFPIKNSGIPINIKNTNAPKEPGTMIL
                     DDESTISYPGTITGIAGKKDFTAITVEKTLMSRDKGFLRKLISVMETNNIFIEHMPSS
                     IDSISLIVSNAKLGANLQKIIEEIRIYCNPDNIVHYNNMALIAVVGRGMIRTKGISAK
                     VFTALAEAGINIRMITQGSSELNIIVGVDNSDFEKAIRAIYKVFN"
     gene            complement(2578..3258)
                     /locus_tag="VK031_07790"
     CDS             complement(2578..3258)
                     /locus_tag="VK031_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HLS53860.1"
                     /translation="MAQLYRKLKDAGYTRSYGSMCRQIRKMKLKKKKKHKTYPKSKYK
                     KPTASYPGQRVQIDVKYVPNECIGFASHHSRYYQITAIDEYSRKRYLELVKENSTYTT
                     SEFLKRLEEKLGFKIDLVQTDNGFEFVNDLDVTNKKTRFEKQLEKMQIKHKRIRPYSP
                     WQNGIVERSHRIDNELFYTKRRFSSFAQMKKAFKRYSRRYNNISRKILGFKTPNEVVS
                     EYFSKNAA"
BASE COUNT         1051 a          740 c          532 g         1373 t
ORIGIN      
        1 cattatcata aagtttaata cctggaaagt ttcttaactc tttttttata tcttctattt
       61 taaatgattt tttcaattcc acattaatag acacactatg gccatatttg actggaattc
      121 ttacggctgt agctgttatg ccaatcttat catcctttag gattttcttt gtctcttcaa
      181 tcatcttcat ctcttctttt gtataaccat tatctaaaaa actatctata tggggcaagg
      241 cattatgttg aatttggtag ggataaaatt ctacctttcc cttttctaaa tccctaagcc
      301 cgccaagtcc agatcctgat actgcctgat aggtggaaaa tataattctt tttattccat
      361 acttatcata aagggctttt aatggtataa cagattgggt agttgaacaa ttaggattgg
      421 caattatatt ctctccctta atatcttctg ggtttatttc aggtaccacc aaaggaatac
      481 cttccttcat tctaaataca ctgctattat caatgaccct tacccccctt tctgctgcca
      541 gcggtgcaaa tctttcactt actgctccgc cagctgcaaa tagggcaatg tctatgtctc
      601 tatcaaagga gtcagacttt aactcttcaa ctacatattc ccttccttta aactttaatc
      661 tttctccagc tgatcttttg gaagaaaata agtataattg gcctactgga aactgtcttt
      721 cctctaaaag ttttaaaatt gtaccgccta ccaagcctgt agcacctact agagctagat
      781 taattttttt cattttccat tcccccttaa ttaaatactt tatatatggc ccttatggcc
      841 ttttcaaaat cactattgtc cacacctact ataatattta gctcactgga gccttgagtt
      901 atcattctga tatttatccc agcttctgct aaggctgtaa ataccttggc tgaaataccc
      961 ttagttctaa tcatccctct tcctaccact gcaatcagag ccatgttatt ataatggaca
     1021 atattatctg gattacaata tattctaatt tcttcaataa tcttttgtag attagctccc
     1081 agcttggcat tagacactat caaagatata gagtctatgc tggaaggcat atgctcaata
     1141 aaaatattat tggtctccat aaccgaaatt agttttctta aaaacccttt gtccctactc
     1201 atcagagttt tttctacagt aatggctgta aaatccttct tacctgcaat accggttatg
     1261 gtgcctggat aggagatggt agattcatcg tctaatatca tggttccagg ctccttgggc
     1321 gcattggtat tctttatatt tataggaata ccagaattct ttatgggaaa caaagcttct
     1381 tcatgtagta caggggctcc catgtaggat aactccctta attccttgta gctaatctcc
     1441 tctattggat ttggctcctt aactatatta ggatcagcca ttaaaaagcc tgaaacatct
     1501 gtccaatttt cataaagaat tgctcctaac ccattggcca caatagagcc ggttacatct
     1561 gaacctcccc ttgaaaaggt cttaatctgc ccatcctttc ttgccccgta aaatccaggt
     1621 attactgccc tatctatatt ttttaccctt tcccttagtc tatgttctgt agctttttga
     1681 tccaattctc cattatctgt aaataagata aagtccttag gatcaaggaa gtgaaaattt
     1741 aggtattttg ataatataag tccatttaaa tactcccccc tgcttccaca gtagtctttg
     1801 gaggaaccct tttctatttt tgcttttatt tcttctaaaa tattatccat gttaaggtct
     1861 atttttagtc cttggcatat tgataaaaac ctttccctaa ttatgttaaa tacttcatca
     1921 aagtttaaac catgggaagc caattgataa cacatataaa ataaatcagt aattttatga
     1981 tcccttccat gccttttacc aggcgccgaa ggaattataa attttcttct tggatcctgg
     2041 tcaatgattt cctttacctt tttaaactgc ctggcatctg ccaaagagct accaccaaat
     2101 ttacttacaa ttatctccat acttttctcc cctttctcta taaaaaatac ccgtccttaa
     2161 taggacgagt attggacccg catccaccta ataaaaatta agtaaattag ctgtaacgta
     2221 agcaaacgca aatccctact ggcttcagga ttgggctcat aggtggtttt cgacttaatg
     2281 taccaaagaa gcctttcacc cggtgggctt ctatctctgt tggcctaatt aaatctactc
     2341 ttcctaatcc tagcctctta atctttactt tgcaatattt tacaacttta tcacaggaaa
     2401 gtcaagggga aattaaaaag gctgtgttta ttcttctaga gtaatcaaac atttgtgaca
     2461 caggcctctg aaaatattct ctaaacaatg ctgtagctat ttttgatttc tagaaattat
     2521 tttgtcaagg aggaaagcgt agcgatcctt gataaaataa ttatttgaaa tatgcttcta
     2581 agcagcattc ttagagaaat attcacttac tacttcattt ggtgttttaa atcctaatat
     2641 ttttcttgag atgttattat atcttctgct atatctttta aatgccttct tcatttgagc
     2701 aaagctactg aaccttcttt tagtataaaa taactcattg tctattctgt gacttctctc
     2761 tactatacca ttttgccatg gggagtaggg tctgattctt ttatgcttta tttgcatttt
     2821 ttctaattgc ttttcaaagc gtgtcttttt attagttaca tctagatcat tcacaaattc
     2881 aaaaccatta tctgtctgca ctagatctat tttaaatcct aacttctctt ctaatctttt
     2941 taggaattcc gatgttgtgt aagtactatt ttcctttact aactctagat atctttttct
     3001 tgagtattca tctattgctg ttatttggta atatcgacta tgatggcttg caaagcctat
     3061 acactcattt ggcacatatt ttacatctat ttgcactctt tggcctgggt agctggcagt
     3121 aggtttcttg tatttgcttt ttggatatgt cttgtgtttc ttctttttct ttaatttcat
     3181 tttccttatc tgtctacaca tggagccata acttcttgtg taacctgcat cttttagctt
     3241 acgatataat tgtgccaagc cttcatgccc atgatatcta tatttatgcc ttattagatc
     3301 tagttcttct tctgtatgct ggtttggatg actatggggt ctagtacttt tatttgctag
     3361 tgaatatatt gtaccgtcat atttattacg ccatcttgaa acctgttgtc tactagtatg
     3421 ataccttctt cctgctttag aattattatc atatttaatt gcatattcaa cgttagtgta
     3481 aaattacttt gggaggaaaa acaaaaaata aaataagagc acctcaatgt gataatatat
     3541 tagttgaaca accataaaaa tcacaaagga gagatgctct tatgaatata attatacaac
     3601 aagttgtaga aataatcatt gattatgtga atttaaatat ggaaactttg ttaaaagaag
     3661 gaaaaggaat atcagacttc atagtaaacc tgaaga
//
LOCUS       DATGSI010000505         1638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_696505, whole genome shotgun sequence.
ACCESSION   DATGSI010000505 DATGSI010000000
VERSION     DATGSI010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696505"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VK031_07795"
     CDS             <1..707
                     /locus_tag="VK031_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HLS53861.1"
                     /translation="DDPMLEDAGIEVELEKTSLVPGESTVAYADYTVTQEDIDSGLIY
                     NIATATGTPPGYDPENPPTDPTDPGYPPVSPPSEVEVPTKQTPDVTLEKAADKEEVTK
                     IGEKLVYTFTITNTGNVTLYDLVLNDPMLGGEIKLAVSSLAPGESTEVSVDHTVTEED
                     GKKNSIVNEADVTGTTKTGESVTDEDQELVRVNIEQPGEELPKTATNMFNYLLIGGLA
                     TLLGAIVLVYARRREA"
     gene            1404..>1638
                     /locus_tag="VK031_07800"
     CDS             1404..>1638
                     /locus_tag="VK031_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53862.1"
                     /translation="MNTFSKITKKHTFKLLSIFLLLFSMISSSFPQQITFAESDPGYQ
                     EGHGSIRNDQGEIDYDEATINKVVSFGNIPGEYF"
BASE COUNT          588 a          254 c          322 g          474 t
ORIGIN      
        1 tagacgaccc gatgttagag gatgcaggta ttgaagttga actagaaaag actagcttag
       61 tacctggtga atctacagta gcttatgcag actatacggt aacccaagag gatatcgata
      121 gtggcttaat ttacaacatc gcaacagcaa caggaacacc tccaggatat gatcctgaga
      181 atccgcctac agacccaaca gaccctggtt atccaccagt aagtccgccg tctgaagtgg
      241 aagttccgac aaagcaaact ccagatgtta ccttagaaaa ggcagcagat aaggaagaag
      301 tcactaagat tggcgaaaaa cttgtctaca ccttcactat aacgaataca ggcaatgtaa
      361 ccttatatga cctagtgttg aacgatccga tgttaggtgg agaaataaag ttagccgttt
      421 ctagtttggc accgggtgaa tctactgagg tttctgtaga ccatacagta actgaagaag
      481 atggaaagaa gaactctata gtaaacgaag cagatgtaac aggaacaacc aaaacaggtg
      541 aatcagtcac tgacgaagac caagaacttg ttcgcgtgaa tatagagcaa ccaggtgagg
      601 aattacccaa aaccgcaaca aacatgttca attatctcct aataggtggt ttagcaacac
      661 tacttggagc cattgtcctg gtctatgcta gaagaagaga agcataagat cataaaaatc
      721 gacatggagt ttaaggactc tatgtcgatt ttttcacatc tttcatcccc attaatttat
      781 gcaatagaaa tagttttgat agggtaaact cattttctat taaagatatt gaagatccta
      841 aaagtatatt ctctctttta tcatccaagt aaaacgaatg actcaccata aaaaaaggag
      901 aatatacgat aaaagattct aaatcggtga aaacaagact gatcaataaa tcttattttc
      961 aatatataaa tatatgataa ttccattagt tctagtataa agtatagaaa agtttattct
     1021 aactattgac attgtacata aattgtatag aatagataga gggttgggaa ataatcccaa
     1081 aaatagacaa tatattacag aggttgaaat agcttgtttt aggggatata agggcatcta
     1141 gtaatatttt ataaaagact aatttccata ttatgtttag gtcatgaaga agacggatgc
     1201 cagataagtc tcttagctta tgaactttcg atatatttag cagaagattt cagaaatgaa
     1261 ctatattttt ttcacatttt ggtgtggtta aaaatatata attaatctgc gtgtaggagg
     1321 ttggtagttt tcattctata taagatatgt tgagagggaa tgtttataga agttaaaggg
     1381 attatatgaa tggaggaata taattgaata cgttttctaa gataacaaaa aaacatacat
     1441 ttaagttgtt atcaattttc ttactgttat tttctatgat ttcctcatcc tttcctcagc
     1501 aaatcacctt tgcagaatca gatccaggct atcaagaagg acatgggtca attaggaatg
     1561 accaagggga aattgattat gatgaggcga caattaataa ggtagttagt tttgggaata
     1621 taccggggga gtatttta
//
LOCUS       DATGSI010000506         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_364461, whole genome shotgun sequence.
ACCESSION   DATGSI010000506 DATGSI010000000
VERSION     DATGSI010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364461"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VK031_07805"
     CDS             complement(<1..266)
                     /locus_tag="VK031_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HLS53863.1"
                     /translation="MLKDITIGQYFPGDTIIHKLDPRIKILIIALFIGSLFFIKSFYP
                     YIFILIFIVTALTLSKVPIKFVLKGLKPLSFVILITFFINVFLT"
     gene            complement(259..1122)
                     /locus_tag="VK031_07810"
     CDS             complement(259..1122)
                     /locus_tag="VK031_07810"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HLS53864.1"
                     /translation="MIVETKDLKYIYNPNTPFEKRALDGINLQIKEGEFIGLIGHTGS
                     GKSTLVQHLNGLIKPSHGQVIIDGTDIAHKEVDMKLIRQKVGLVFQYPEYQLFEETVY
                     KDIAFGPKNLGLKEDKVHKRVVEAMEIVGLDYQALKDRSPFELSGGQKRRVALAGVIA
                     MRPKVLILDEPTAGLDPHGRDEILKEIYKLFQKEKITIILVSHSMEDVARLVDRILVM
                     DEGKIAMDGKPRDIFKRAEELEKMGLGIPQITKFMQEYKKKGKQIRDDILTVEEAKVE
                     LTKYLRSKKDA"
     gene            complement(1119..>1550)
                     /locus_tag="VK031_07815"
     CDS             complement(1119..>1550)
                     /locus_tag="VK031_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS53865.1"
                     /translation="MIDYKKHAPHLLSGGQKQRVAIAGILAMRPDCVILDEPTSMLDP
                     IGRIEVMETIEKLNKEEGKTIVLITHYMDEAVLGDRIIVLNKGEILMEGTPKDVFKQV
                     DRIKEIGLDVPQVTELVYELRKEGIDIRDDVLSVEELLDIL"
BASE COUNT          415 a          338 c          216 g          581 t
ORIGIN      
        1 ttagttaaga aaacattaat aaagaaggtt attaatataa caaaggataa aggtttaagc
       61 cctttcagta caaacttaat cggaaccttt gataaagtaa gggctgttac gataaatatt
      121 aagataaata tatagggata aaagctctta ataaaaaata aagaacctat gaatagagcg
      181 attattaata tttttatcct tggatccagt ttgtgaatta tggtatctcc tggaaaatat
      241 tgacctatgg ttatgtcctt aagcatcttt tttactcctt aaatatttag ttaattctac
      301 ttttgcctct tctaccgtta atatgtcatc ccttatttgc ttgccctttt ttttatactc
      361 ttgcataaat ttagttatct gaggtattcc cagccccatc ttttctaatt cctctgccct
      421 tttgaatatg tcccttggtt tcccgtccat ggcaattttc ccttcatcca ttaccagtat
      481 cctatctacc aacctggcca catcttccat gctatgtgat actagaataa tggttatctt
      541 ctctttttgg aatagtttgt agatctcttt tagtatttca tccctgccat gtggatcaag
      601 gcctgctgta ggttcatcaa gaataagaac cttaggcctc atagctatca ctcctgccaa
      661 ggctacccgc cttttctgac ccccacttaa ctcaaaaggg gatctatcct ttaaggcctg
      721 ataatcaagt cctacaatct ccatggcctc tactaccctt ttatggactt tatcctcttt
      781 taagcctaga ttcttgggac caaaggctat atccttatat actgtttctt caaataattg
      841 atattctgga tattgaaata ccagacctac cttttgccta attaacttca tatccacttc
      901 cttatgggct atatctgttc catctattat aacttgacca tggcttggtt tgattaggcc
      961 atttagatgt tgaaccaagg tagattttcc tgatcctgta tgtcctatta gcccaataaa
     1021 ttctccttct ttaatctgta aatttatccc atctagggct cttttttcaa agggtgtatt
     1081 agggttatat atatatttta aatctttagt ttcaactatc atagtatatc taacaactcc
     1141 tctacggaaa gtacatcgtc ccttatatct ataccttctt ttcttaattc atatactaat
     1201 tctgttacct gcggcacatc taaacctatt tcctttattc tatctacctg tttaaataca
     1261 tcttttggtg tgccttccat caggatctct cccttgttta agacaattat cctgtctcct
     1321 aatacagcct catccatgta atgggtgatt aatactatag tttttccctc ttccttgtta
     1381 agcttttcta tagtctccat aacttcaatt ctacctatag gatccaacat ggaggtaggc
     1441 tcatctaaaa ttacacagtc tggtctcatg gccagtatac ctgctatggc taccctttgt
     1501 ttttgtcctc ctgataaaag atggggcgca tgcttcttat agtctatcat
//
LOCUS       DATGSI010000507         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_506583, whole genome shotgun sequence.
ACCESSION   DATGSI010000507 DATGSI010000000
VERSION     DATGSI010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506583"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1474)
                     /locus_tag="VK031_07820"
     CDS             complement(<1..1474)
                     /locus_tag="VK031_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HLS53866.1"
                     /translation="MNQKYEVLDKSGKWYKINANGRTGWVHGDYVKLVEEAAKPEPIE
                     KVSFKSLTISGSKNVGDKHNIKAEASSKNGVLYKFVIKDLETNKSTVLRDFNKSNTVS
                     WTPNKAGKYSYQVYMKDSKDKDGQKSIKQSKDITINPIPPAKLEEFTIEGTGYATDTH
                     IIKAKASSQNQVLYQFRIMDLNSGKTTIIRDYDKSNTAEWIPEYAGQYLYEVRIKDSN
                     STKDADASASKEVTINSYIFYSTSKYIQSLEEMINEQMKRNPQTDKGGGGWRSANREE
                     LEEYINPEHFLQFSPDLGDNSNKIATITANPRLNVRKGPGTGYQILGQVNYGETYVIL
                     AESKNWYKISVDGIEGWISGAYTKVDVGYSKLRAIEVTTDTLNVREKATTSSPVLKQV
                     KKGDVFIVLDEANGWYQVNVGDTKGWISGSYTRPTNNVPREMYQFLVLSGSSGIGASD
                     LNKELKGKGILEGMGQAFIDAGKKYNINEIYLLSHALLETG"
     gene            2181..3134
                     /locus_tag="VK031_07825"
     CDS             2181..3134
                     /locus_tag="VK031_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HLS53867.1"
                     /translation="MNRKKKNTNKKRTNAQIFRRVFLISLVVFTILGMGFVYVYRLLD
                     KMNPVDIAEDDEALGITEDTFENKKVVNIALFGVDRRSSSGVGRSDSIMIATIDREHK
                     KIKLTSLLRDLYVDIPGRGMDKLGHAYAYGGPELAIRTINHNFNMNIREFATVDFDSF
                     EKIVDILGGIEIDIKPEEVRYVPGSKAGTQVLNGKQALAYSRIRKVGTDYQRTERQRT
                     VMEHIINKGLRAGITKYPSLLNAVLPHVDTSISKTEAFTLGTSTLTSGIKNVDQYRIP
                     VKGYVKSQMINGRSYEVPDTLEDNIKFLHKFIYEDEKELGE"
BASE COUNT          919 a          604 c          527 g         1129 t
ORIGIN      
        1 tacctgtttc cagtaacgca tgggataata aatatatttc atttatattg tatttctttc
       61 ccgcatctat aaaggcctgt cccattcctt ctaatatgcc cttgcccttt aactccttgt
      121 taaggtcact ggcaccaatt ccagaacttc ccgataggac caagaactga tacatttctc
      181 taggtacatt atttgttggt cttgtataag acccgcttat ccatcctttg gtgtcgccta
      241 catttacctg ataccatcca tttgcttcgt ctaaaacaat aaatacatca ccttttttta
      301 cctgtttcaa caccggactg gatgtagtag ctttttccct aacatttaaa gtatcagttg
      361 taacttcaat agccctaagc ttggaataac ctacatctac ctttgtatag gccccgctta
      421 tccaaccctc aattccatct acacttatct tgtaccaatt ctttgattca gctaatataa
      481 catatgtttc accatagttt acttgaccaa ggatctgata accagtacca ggtccttttc
      541 ttacattgag tcttggatta gcagtaattg tagctatttt attggaatta tcccctaaat
      601 ctggagaaaa ttgaaggaaa tgttcaggat taatatattc ttctaattcc tctctatttg
      661 ctgatctcca gccaccgcca cctttatctg tctgtggatt tcttttcatc tgttcattaa
      721 tcatttcctc aagactttgg atatatttac ttgtgctata aaatatataa ctatttatgg
      781 tgacttcctt agaggcacta gcatcagcgt cttttgtact atttgaatcc tttattctta
      841 cctcgtataa atactgtcct gcatactctg gaatccattc tgctgtattt gatttgtcgt
      901 aatcccttat tatagtagtc tttccactgt ttaaatccat aattctaaac tgatataata
      961 cctgattttg actactggcc ttagccttta taatatgagt atctgtagcg tatcctgttc
     1021 cttctatagt aaattcttct agctttgcag gtgggattgg attgattgtt atatcctttg
     1081 actgcttgat gcttttttga ccatccttat ccttactatc tttcatgtaa acctgatatg
     1141 agtatttacc agccttatta ggagtccaac ttacagtatt agacttatta aaatctctta
     1201 atactgtact tttatttgtt tctaaatctt taataacaaa cttataaagt accccatttt
     1261 tgctgctagc ttctgcctta atattatgct tatctcccac attctttgat ccactaattg
     1321 tcaggctctt aaagcttact ttctcaattg gctctggctt agctgcttct tctaccagct
     1381 ttacatagtc tccatgtacc cagcctgtcc ttccattagc atttatcttg taccatttac
     1441 ctgatttatc tagcacttca tacttttggt tcaatcttac ttgagtaagt ctgctgctgg
     1501 atgtagtagc cttggcccta acatttagga ctgctgtggt tacctggatt acatctccgg
     1561 tgcccttctt gctaatatca atatagtcac cgtaaaccca gcctgtctta ccattggccc
     1621 ttatcttatg ccatttgcct gatgtatcta gtatttcata tgtttcgttt tttcttactt
     1681 gagtaactat gctactggat gtggtagcct tggctctaac atttaagacg cttactgtaa
     1741 ccttgccggt cttggtactt ttattagtag catatgaggt atggtgatta aggcttgtac
     1801 taaagaatag taataaagat agtagaataa ggacttttac aatccttttt ttgttcagga
     1861 ttttcccccc cttcctttat gattcttcca acttatactt ctatttaaaa gtataaattg
     1921 gaaaatggtg taaacatatt atatcctaag aaactaacac tttcaagcat tagagaatgg
     1981 ataaaagctt aatatattta agccttttat taatttaact tatccttgga cagcctattt
     2041 caactataga aaaaattcat caatttgtaa tatctaattt attgtattat ccttcccatc
     2101 tggtataata tcaatatgca ttagcagttg aatgcttatt tttgcgaact tttcgcatac
     2161 tgaagatgag ggggaaaata atgaatcgaa agaaaaaaaa tacaaataaa aaaagaacca
     2221 atgcccaaat atttaggcga gtctttttaa tttctcttgt agtatttact attttgggca
     2281 tgggctttgt atatgtatac aggttattag ataagatgaa tccagtagat attgctgaag
     2341 atgatgaggc tttaggcata actgaggata cttttgaaaa taagaaggtg gtaaatattg
     2401 ccttgtttgg agtagataga aggtcctctt ctggagtagg tagatcagac agtattatga
     2461 tagccaccat tgatagggag cataaaaaaa tcaaacttac ctcgctatta agggatcttt
     2521 atgtagatat accaggcagg ggaatggata agcttggcca tgcctacgcc tatggtggtc
     2581 cagagttagc cattaggact attaaccaca actttaatat gaatattcga gaatttgcca
     2641 ctgtggattt tgatagtttc gaaaagatag ttgatattct tggtggtata gaaatagata
     2701 tcaaacctga agaggtcaga tatgtaccag gcagcaaggc aggtacacag gtacttaatg
     2761 gtaagcaggc cttggcctat tctaggataa ggaaggttgg gactgactat cagagaactg
     2821 aaagacagag aactgttatg gaacatatta taaataaggg cttaagggct ggtataacta
     2881 agtatcctag tctcttaaat gcagtactac cacatgttga tacaagtatt tctaaaacag
     2941 aggccttcac acttggaaca tctactttaa catcaggtat caaaaatgtg gaccaatacc
     3001 gtattccagt caagggatat gttaaaagtc aaatgataaa tggtagatct tatgaagtac
     3061 ctgatacact ggaagataat ataaagtttt tacacaagtt tatatatgaa gatgaaaaag
     3121 aactaggtga gtagcctagt tctttttcta actctgattc tagcctctct tgatgtgca
//
LOCUS       DATGSI010000508         1650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_316005, whole genome shotgun sequence.
ACCESSION   DATGSI010000508 DATGSI010000000
VERSION     DATGSI010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316005"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1533
                     /locus_tag="VK031_07830"
     CDS             67..1533
                     /locus_tag="VK031_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53868.1"
                     /translation="MEYIDDIYNYWLNEYVTLRREVISTLLSDYSAPNRYRIGNDFKF
                     VEERPEYIDQTIWDKLFIKDRMQIPEFTYGPDRPPYSIYGNQIKEGVLLCTNKLCGYF
                     YIIECNDVIIPEGCLPTSYENALGFTQRFISTSRIDNRAKIFLDTAIEVYGKEDVWVG
                     FTSAHPYLSASDRFDEGFYMIIHHGDVWVEDNTGLRYKARNLVTTINLNRRGIIGNTG
                     SVYGMSLTKDNLLIGNNFDHPHIDISRNAFEFSNVCLGSESSISRQIGVISNAEEGDL
                     SEEECLSFWFHLSALLEEESKEGVPFHYIMGNILDEEEMGLEVSFSSIVSAIVKGYVD
                     INCDIVGVTLRTQVCEASLIDYLNEATDDGEIYRSPRGSYHRKELSKRLADVELGIVG
                     HFPFFDNYIPIEVTEVCEDNYDLSVMDEWDTAITSSIPRSVISDVEIMLDKVVNFKLE
                     YDVTKRKKEYKGNKELATLIEVQRSTNIEDLAKFIGED"
BASE COUNT          545 a          249 c          341 g          515 t
ORIGIN      
        1 tctaaataac tataaagagg ctctcagata tagtctggga gcctctatta tttaatacaa
       61 tactatatgg aatatataga cgatatttac aactactggt taaatgaata tgtcaccctc
      121 cgccgtgaag taatatcaac cctcctatca gactacagtg ctcctaatag atatcgaata
      181 ggtaatgatt tcaagtttgt agaagaaaga cctgaatata tagatcaaac tatttgggat
      241 aagctgttta taaaagatag aatgcagatc ccagagttca cgtatggacc agacagacca
      301 ccatattcaa tttatggtaa tcagataaaa gaaggagttc ttctgtgtac taataagcta
      361 tgtgggtatt tctatataat agagtgtaat gatgtcatca tccctgaagg ttgtctccct
      421 acctcctatg agaatgctct tggctttaca caaagattca tatctacatc aagaatagat
      481 aatagagcta agatctttct tgatactgct atagaagtat atgggaaaga ggatgtatgg
      541 gtaggattta caagtgctca tccatatttg agtgctagtg atagatttga tgaaggcttt
      601 tatatgatta ttcaccacgg tgatgtgtgg gtagaagata acactggtct tagatataag
      661 gctaggaacc ttgttactac tattaactta aacagaagag gtattatagg taatactggt
      721 tctgtatatg gaatgtcctt gactaaagat aacctactta taggtaacaa ctttgatcat
      781 cctcatatag atatatcacg caatgctttt gaattttcta atgtttgctt agggtctgaa
      841 tcatctatca gtaggcagat aggagtcata tctaatgctg aagagggtga tctctctgaa
      901 gaggagtgtt tatctttttg gttccatctc tctgcgctac tagaagaaga gtccaaagag
      961 ggtgttccct ttcactatat catgggcaac attcttgatg aagaagagat gggcttagaa
     1021 gtaagcttta gttctatagt atctgctata gtcaaaggtt atgttgatat taattgtgat
     1081 attgtgggtg taactctaag aacccaagta tgtgaagcta gtctaataga ttacctaaat
     1141 gaggccacag atgatggtga aatatataga tcacctagag gaagttatca tcgtaaagag
     1201 ttaagtaaaa gactagctga tgtagaactc ggtatagtag gtcacttccc tttctttgat
     1261 aattatatac cgatagaagt aacagaggta tgtgaagata attatgacct atctgttatg
     1321 gatgagtggg atacagccat tacttctagt attccacgat cagttatttc agatgtagaa
     1381 atcatgttag acaaagtagt caactttaaa ttggagtatg atgtcactaa gcgtaaaaaa
     1441 gaatacaaag gtaataaaga actcgccacc cttattgagg ttcaaaggtc aaccaacatt
     1501 gaggatctcg caaagtttat tggagaagat tgattacttg cacctcaatg taggaggtaa
     1561 agagtggagt ggtagacttg tatataagat catagaggga tctcttggtg atccagacaa
     1621 tatgattata gaagctactg atattttcct
//
LOCUS       DATGSI010000509         1587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_413513, whole genome shotgun sequence.
ACCESSION   DATGSI010000509 DATGSI010000000
VERSION     DATGSI010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413513"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..592)
                     /gene="citX"
                     /locus_tag="VK031_07835"
     CDS             complement(56..592)
                     /gene="citX"
                     /locus_tag="VK031_07835"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HLS53869.1"
                     /translation="MQEEKILMDILDAREARALRQKELLKEFKTSLVSFTLNIPGQVK
                     DSPLYREIHREGERLLVEKLEKIGVAILHKERRYKVTGPEFFLLADKDALELKKLAME
                     IEEENPLGRIFDMDVFDKGHNQVSRSSLKRRPRRCLICNRPAKECIREGNHNLEELLK
                     RIEEISKKYFKASKREEI"
     gene            complement(697..>1587)
                     /locus_tag="VK031_07840"
     CDS             complement(697..>1587)
                     /locus_tag="VK031_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HLS53870.1"
                     /translation="IYCTANGAKILKAHFHEDWNFVEVKTGDTLNIGESTLTFIEARM
                     LHWPDSMMTYMDSDAILFSNDAFGQHYASDQMYNDTVDQCELFQEAIKYYANILTPFS
                     PLVTNKIKEILGFNLPLNMIATSHGIIWRDNPAQIVEKYLEWADNYQENQVTLVYDTM
                     WNATRRMAEAIAEGIKSIDNSITIKLYNAAKTDKNDIITELFKSKAFLFGSSTVNRGI
                     MNATGGLLEMLRGIGFKNKKAAAFGSYGWSGESVKIIEEKLKDAKLEVIEGGPRALWN
                     PDKESIEKCIEFGKEFARSL"
BASE COUNT          412 a          355 c          241 g          579 t
ORIGIN      
        1 tcataaagca tggattttat acacaaattt gctatgtaat tacaaaactt atccttcata
       61 tttcctccct tttacttgct ttaaaatatt ttttagatat ttcctctatc ctttttaata
      121 attcttctaa gttatgattt ccctccctga tacattcctt ggcaggccta ttacatatta
      181 ggcaccgtct aggtctcctc tttaaactag acctacttac ctggttatgg cccttatcaa
      241 atacatccat atcaaatatt ctgcctaaag gattttcctc ttctatttcc attgccagct
      301 tttttaattc taaggcatcc ttatctgcca gcaggaaaaa ctctggtcca gttaccttat
      361 atctcctttc tttgtggaga atggctaccc ctatcttttc taatttctcc accaaaagcc
      421 tttccccttc cctatgtatt tccctataaa gggggctatc ttttacctgt cctggtatat
      481 ttaaggtgaa ggataccagg cttgttttaa attcttttag caattctttt tgcctcaggg
      541 ccctggcttc cctggcatcc aatatatcca ttagaatttt ttcttcctgc atcttatcct
      601 ccttgattta actttacata tattatagac tagcaggtgg aaaaaataaa gaaggatagc
      661 aggctatcct tctttagcta aaatagattt tctttcttat aggcttcttg caaactcttt
      721 tccaaactct atacattttt caatggactc cttgtctgga ttccacaggg ccctaggccc
      781 tccttcaata acctctagtt tggcatcctt tagtttctcc tcaattatct ttacagattc
      841 accggaccaa ccgtaggaac cgaaggctgc tgctttttta tttttaaagc cgattcccct
      901 taacatttcc agcaatccac cagtagcatt cattatccct ctgtttacag tggaagaacc
      961 aaataggaag gctttagact taaataattc tgtaattata tcgtttttgt cagtcttggc
     1021 cgcattatat agcttgatgg taatactatt atctatagac ttaattccct cagctatggc
     1081 ctctgccatt cttctagtgg cattccacat ggtatcatat actagggtta cctggttttc
     1141 ctgataattg tctgcccact ccaagtattt ttctactatt tgggctgggt tatctctcca
     1201 gataatcccg tggctggtgg ctatcatatt taaaggtagg ttaaagccta agatttcttt
     1261 aatcttattg gtcaccaaag gactaaaggg agttaaaata ttggcatagt atttgatagc
     1321 ttcttggaaa agttcacact ggtctactgt atcattatac atttgatcag atgcataatg
     1381 ttgcccaaag gcatcattac tgaaaagaat ggcatctgag tccatataag tcatcatact
     1441 gtctggccag tgtaacatcc ttgcttctat aaaggttagg gtagattcac ctatatttaa
     1501 ggtatctccc gtttttacct ccacaaaatt ccaatcttca tggaaatggg cctttaagat
     1561 cttggctcca ttggctgtac agtaaat
//
LOCUS       DATGSI010000510         1073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_132434, whole genome shotgun sequence.
ACCESSION   DATGSI010000510 DATGSI010000000
VERSION     DATGSI010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132434"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VK031_07845"
     CDS             <1..861
                     /locus_tag="VK031_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53871.1"
                     /translation="LLKTYDKRKENPNGPVTCKRNDILDLNVGDYLDWADKVEEGFYE
                     AAQFLMEQYIFTRRDVPYPTQLVPLAAIFTVLGNRVYNKTIREKIGQWYWSGVFGELY
                     GSAVESRFAKDLPQVLDWLEGGTEPATVNDANFEIDRLLTMRTRNSAAYKGVHALLMK
                     KGCHDFMSGVPIDLQTYFSNSIDIHHIFPIRYCNRNNIQPAIYNSIVNKTPLSSTTNR
                     SIGGSAPSEYLEKLENQRNIPYEVLNNILETHLIDVDSIRSDDFENFFEKRKESLFNL
                     ILEAMGQENN"
     gene            905..1051
                     /locus_tag="VK031_07850"
     CDS             905..1051
                     /locus_tag="VK031_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53872.1"
                     /translation="MWEFSQGVVNIFLVIITLAMKESENKWDNLSRLDHSFLQKYFHI
                     LNSK"
BASE COUNT          391 a          160 c          209 g          313 t
ORIGIN      
        1 ttattaaaaa catatgacaa aaggaaggag aaccctaatg gcccagttac ttgtaaaaga
       61 aatgatattt tagacctaaa cgtaggagac tatttagact gggcagacaa ggtggaagaa
      121 ggattttatg aggcagcaca atttttaatg gagcaatata tattcaccag aagggatgta
      181 ccatacccaa ctcaattggt gcccctagct gcaattttta ctgtgttggg taatagggta
      241 tacaataaaa ctatcaggga gaaaattggc caatggtatt ggtctggagt attcggagaa
      301 ctatatggtt cagcggtaga aagtagattt gctaaggatt taccacaagt tttagattgg
      361 ttagaaggtg gaactgaacc tgccacagtt aatgatgcaa actttgaaat tgataggcta
      421 ttaactatga gaactagaaa tagtgccgct tacaagggag tacatgcact tttaatgaaa
      481 aaaggttgcc atgactttat gtctggagtt cctatagatc ttcaaacata tttcagtaat
      541 agcatagata tccatcatat tttccccatt agatactgta acaggaacaa tatacaaccg
      601 gctatatata attcaatagt taataaaaca cccctatcat caacaactaa taggtcaatt
      661 gggggaagcg ctcctagtga atacttggaa aaactagaaa accaaagaaa tataccctat
      721 gaggtattaa acaatattct agaaactcat ttaatagatg tagatagtat acgatcagat
      781 gactttgaaa acttctttga gaaaagaaaa gagtcattat ttaatcttat cttagaggcc
      841 atgggacagg aaaataatta gcattttgtt tataagactt atcctttata cttaggaggg
      901 caatatgtgg gaatttagcc aaggagtagt caatatattt ttagtcataa tcaccttggc
      961 catgaaagaa agtgaaaaca aatgggataa cctatctagg ttggatcatt ctttcttgca
     1021 aaaatacttt catattttaa atagtaagta acctttaata ggcaagacat ggt
//
LOCUS       DATGSI010000511         8134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_316234, whole genome shotgun sequence.
ACCESSION   DATGSI010000511 DATGSI010000000
VERSION     DATGSI010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316234"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VK031_07855"
     CDS             complement(<1..443)
                     /locus_tag="VK031_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53873.1"
                     /translation="MRMLIHDLEDEEKLAEVYPYPLENMVIISNEKEIHYCLRCFACW
                     VKTPAQCIIQDNCGNLGQSLSKCKEISIVSKCCYGGFSPFVKNVLDRSISYLHPYFEV
                     KMGKLHHKRRYKNQIDLYIYFYGEQLTEEEKKTAKDLVEANAVIFG"
     gene            complement(785..1735)
                     /locus_tag="VK031_07860"
     CDS             complement(785..1735)
                     /locus_tag="VK031_07860"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HLS53874.1"
                     /translation="MLDFTLLGSGGSMPSLKRHFSAALINFQGRKILIDCGEGTQVAM
                     RKLHTGFKSIDLICFTHFHGDHIFGLPGLLSTISKSGRKEPITIIGPHGLNKVINGFL
                     ATMSRLPFELQLIEEPRDELYLTIENQLLTISPSRQARNKKIILTNMELDHSLPCLGY
                     SFYIVRRPEFNLEKAMKLGIAKEHWSRLQKGEDVYIGDKLISPGEVLGKERKGIKLSY
                     ILDTRPIEGITDFVKGSDLFVCEGTYGSDDDLDKAISNKHMTFREAAELAYKAEVKKL
                     LITHFSPAMEEPGIYAENATDIFKNTIIGYDGFRKKLSYD"
     gene            1914..2279
                     /locus_tag="VK031_07865"
     CDS             1914..2279
                     /locus_tag="VK031_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53875.1"
                     /translation="MGFKNKFLIFSIVLATLIPTFVFSDNLVEPEDTNLEKEIEPEGG
                     LTFLRVEQEEDKIPEDVKEEILNLKQKLKNGQINKEEFKEELEKLIPDSQKNIFKNNL
                     KERLPQDIKENIDKLKISL"
     gene            2392..3102
                     /gene="sfsA"
                     /locus_tag="VK031_07870"
     CDS             2392..3102
                     /gene="sfsA"
                     /locus_tag="VK031_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HLS53876.1"
                     /translation="MKYNKVVDGIFVKRPNRFIAHVLINDKEEKVHVRNTGRCKELLI
                     PGAKVILEDCSNNPNRKTKYSLIAVWKEDILVNMDSQIPNKVIFNSLEANQINGLEGL
                     KQVKREVTFGQSRYDLYFESQFEQGFIEVKGVTLENNGIAMFPDAPTERGTKHVLEMV
                     EAVKKGYKGIIFFLIQMKGPKVFRLNWKMDKKFSEAVVFAKEKGVEILAYDSHVTENT
                     IILGQPIKLDLFNQDPLF"
     gene            complement(3153..4346)
                     /locus_tag="VK031_07875"
     CDS             complement(3153..4346)
                     /locus_tag="VK031_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLS53877.1"
                     /translation="MDYPIVFIPGLFGSLGDEVIPGTGNFSFGLAEGIYKPFIKILNS
                     IGYVEGENLFVSYYDWKNSILKSTDKYLDKDIKKVKKKTGSKKVILIGHSLGGLLGRS
                     YMTYFSSSSVDKLIMIGTPNFGAANAYYFWSGGRLPYPRVERNILYNGIKLGFILYFQ
                     LFHNEGYLKPLRKLFPVVQDLLPSYDYGNYLYYEEDGERKEIPIEKMSVKNPFLSKLN
                     QRPINRANTFLITGKGEETSMEFLVDLNKGKQNIWVDGEPIGVNKTNYGDGTVTTFSS
                     LGNLGQQYIIIEGDHTNILYRSQEYLAKVLNKPISERIFIKEVEKLHLIFADNCREME
                     IITSDTSRISTEKSDIRDKRLQVINLGNNRFWIMAKGDKDLEVGLNMTNKVKREANIY
                     QTSLG"
     gene            complement(4407..4943)
                     /locus_tag="VK031_07880"
     CDS             complement(4407..4943)
                     /locus_tag="VK031_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLS53878.1"
                     /translation="MRWETIETEWLEWDAPWEMEEGPFNLEEYRRLMTKRLSRKLDPN
                     EFRYSFQICINDESKKHIGWVNAYYIDNDYQYTEGQGYLTIGINIPDLNSRRKGYATE
                     AWLLYIRYALNNGYKDIFTQTWSGNFRVIGLIEKLRFKECMRKKDLRKVRGKLYDGLT
                     FKLDLNSFKKAYNGGYKK"
     gene            complement(5071..5517)
                     /locus_tag="VK031_07885"
     CDS             complement(5071..5517)
                     /locus_tag="VK031_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53879.1"
                     /translation="MKRLVVFALCITIFFVLLGCGRKEKNKILDFKAKDVENIEIYRF
                     DIPVEAEGMLVTEREDIEKVMKAFWQIKIQGDAKDLYLVGGELISFRFNLKNGEEFTL
                     AYLGRVMLKTDKGDYRVWAEEVGDLWQGLDYPKSYVSEEKLPKFNN"
     gene            complement(5598..5858)
                     /locus_tag="VK031_07890"
     CDS             complement(5598..5858)
                     /locus_tag="VK031_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53880.1"
                     /translation="MILAAGPLLSLIFGIVLLYVARNMEDKTLMGIILIREMIILTRS
                     YQLYMFISTSIPIIYPKAWMGYGGYPSDGYQIRKLLKSKNTY"
     gene            complement(5979..6128)
                     /locus_tag="VK031_07895"
     CDS             complement(5979..6128)
                     /locus_tag="VK031_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53881.1"
                     /translation="MGLVNFTLEIVLTGVFFYVIFLFSTIVHEFGHALWALILTKENV
                     KIILG"
     gene            complement(6169..6660)
                     /locus_tag="VK031_07900"
     CDS             complement(6169..6660)
                     /locus_tag="VK031_07900"
                     /inference="COORDINATES: protein motif:HMM:NF028342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HLS53882.1"
                     /translation="MKLRRKRIGSKASGKGVLLSEESMNKYIEITIIIKQREKDKMFF
                     VFGISTKEKELDFSQPIVCPTCEAYGRLEAFMTYTYFSLFFIPLFRWNKKYFIKSVCC
                     GSLYTIDRELGRAIERGEITRIDESDLEVVDPGYGRRRTCRNCMSPLEKDYEYCPRCG
                     MKA"
     gene            complement(6731..7312)
                     /gene="pyrE"
                     /locus_tag="VK031_07905"
     CDS             complement(6731..7312)
                     /gene="pyrE"
                     /locus_tag="VK031_07905"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HLS53883.1"
                     /translation="MISKKRIKEIFEELEVITYGHFLFTSGRHGDTYMQCAKLLKHPD
                     YTREIVEPIVDRFKGDKIDIVIGPAIGGIILAYEFARQLGVQNFFAERENNVMTLRRG
                     FRIPKGARVLVVEDVITTGGSVREVIDIVEREEGELVGVAVLVDRTGGQVDFGTKFVT
                     ALSEELVSYSPKDCPLCREGRLPLEKPGSKKFA"
     gene            complement(7352..>8134)
                     /locus_tag="VK031_07910"
     CDS             complement(7352..>8134)
                     /locus_tag="VK031_07910"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HLS53884.1"
                     /translation="GISSKGLTLEMREGNKGRRIHETAAGIMNSIGLQNPGVQGFINN
                     ELAYLEEKDTVILANLGGGRIEDYVIGAQLLDKTGIDFIELNISCPNVKKGGMAFGIK
                     CDPAYEVVKATREVTSKPIIVKLSPNAENIQEMAVTCVKAGADGLSLVNTFNALAIDI
                     YKRKPIFDNITAGLSGPCIKPIALRMVYEVSKVVDVPIIGIGGITCAEDALEFIMAGA
                     TAIQVGSANFIKPDISLDIIKGIEDFMKRKGIKSLEEIRGII"
BASE COUNT         2514 a         1658 c         1082 g         2880 t
ORIGIN      
        1 ccaaaaataa ctgcatttgc ctctactaaa tccttagctg tcttcttttc ttcttctgta
       61 agctgttccc cgtaaaaata aatatataaa tctatctgat ttttgtatct ccttttatgg
      121 tgcaattttc ccatctttac ctcgaagtaa gggtgcaagt aggatatact tctatctagt
      181 acgtttttca caaaggggct gaacccacca tagcaacatt tacttactat acttatctcc
      241 ttacacttag acaaagactg gcccaggttt ccgcaattat cctggataat acattgagct
      301 ggtgttttta cccagcatgc aaaacacctc aagcaataat gtatttcttt ttcattggaa
      361 attatgacca tgttttctaa gggataagga tatacctcag ctaacttctc ttcatcttcc
      421 agatcatgta taagcatcct catgctaaaa cctacctttc actttaaact aaaccagcta
      481 gacagggctt tgattgttaa ttgtcaaaca tcacttgcta taaatatagt ctttccccat
      541 tccctaaata aaccttcttc aattacaatt atatcatatt ttcagtaaat tataatgaat
      601 tataagtttt tatataaatt atatattcct taaggagcaa attaaaaatc taaccttaag
      661 aaaaacttta tacctagaaa attagtctta tcttgattgt gtattcttta tattcaacca
      721 atacaccctt tgttaatcca aatataaata aggcctttgg ccaactacca accccatttt
      781 ttaattaatc atagcttagt ttttttctaa atccatcgta tccaatgata gtatttttaa
      841 aaatatctgt tgcattttca gcatagattc ccggttcttc catggctggg ctaaaatgtg
      901 taattagtaa ctttttcact tctgccttat atgccaattc tgctgcctct ctaaaagtca
      961 tatgtttgtt gctaatggcc ttgtccaaat catcatcact gccgtaggtt ccttcacata
     1021 caaataaatc agatcctttt acaaaatctg ttatcccctc tataggtctt gtatctaaaa
     1081 tatagctaag tttaattccc ttcctttctt ttcctaaaac ctcaccgggg ctaataagtt
     1141 tatcccctat atatacatcc tccccttttt gtaacctaga ccagtgttct ttggctattc
     1201 ccagtttcat ggccttttcc aaattaaatt ctggccttct tacaatataa aaactataac
     1261 ctaggcaagg caaggagtgg tccagttcca tattggttag gattattttc ttattcctgg
     1321 cctgtctgct gggggaaata gtaagtagtt gattctcaat tgttaaatat agttcatccc
     1381 ttggttcttc tattagctga agttcaaagg gtagcctaga catggtggct aaaaatccat
     1441 taattacctt atttaatcca tgaggaccta taatggtgat aggctctttt ctcccacttt
     1501 tacttatagt tgaaagcaaa cctggcagac caaaaatatg gtctccatga aaatgtgtaa
     1561 aacatataag atctatactt ttaaatccgg tgtgaagttt tctcatggct acttgggttc
     1621 cttcaccaca atctatcaat atttttctgc cttgaaaatt aataagtgca gctgaaaaat
     1681 gtctctttaa agatggcatg ctaccaccac tgcctaataa ggtaaaatcc aacaaaatga
     1741 tcctcctttt ttaaaaaaat atttctttta ttgtatcata ttgttttttc tttatttcat
     1801 tataaattta aaaattttct tatatatcca taaatcttat aacattatta atttactttt
     1861 caacataata atattaggct tcattattta aaaaagaaag gagctaattt tttatgggct
     1921 ttaaaaataa gtttcttatt ttttctattg ttttggctac cttaattcct actttcgttt
     1981 tttctgataa tttagttgaa cctgaagata caaacttaga aaaagaaata gagcctgaag
     2041 gaggcctaac ttttttaagg gtagagcaag aagaagataa aattcctgaa gatgtgaaag
     2101 aggaaatttt aaacttaaaa caaaaactaa agaatggtca aattaataaa gaagaattta
     2161 aagaagagtt ggaaaaatta atacctgata gtcaaaaaaa tatttttaaa aataatttaa
     2221 aagaaagatt accacaagat attaaagaaa atatagataa attaaaaatt tccctttaat
     2281 cattatctca caaccggtct tcggttgtga gttttttata tttcatcttc ttgcttgttg
     2341 aaatttaaag tgatacaatt tatagagatt attattaata aaggagtttc aatgaaatat
     2401 aataaggttg tagacggtat ttttgttaaa agacctaata ggtttatagc ccatgttcta
     2461 ataaatgata aggaagaaaa agtccatgtt agaaacacag gaaggtgtaa agagctttta
     2521 ataccaggag ccaaggtgat tttagaagat tgttccaata atcccaatag aaagactaag
     2581 tattccttga tagctgtttg gaaggaagac atcttagtta atatggattc acaaatacct
     2641 aataaggtca tatttaattc tttagaagct aatcaaataa atggtttaga aggtttaaaa
     2701 caagtaaaaa gggaagtaac tttcggtcaa tcaagatatg atctttattt tgaaagccaa
     2761 tttgaacaag gctttataga ggtcaagggg gtaaccttag aaaataatgg tatagccatg
     2821 ttccccgacg ctcctactga aagaggtact aagcatgttt tagaaatggt ggaggctgta
     2881 aagaagggat acaaaggtat tatatttttc ctcatccaga tgaaagggcc taaagttttt
     2941 cgtctaaact ggaaaatgga taaaaaattt tcagaagctg tagtatttgc taaggaaaaa
     3001 ggtgtagaaa tacttgctta tgacagccat gtaactgaaa ataccatcat acttggtcag
     3061 cctattaagc ttgatttatt taatcaagat cccctatttt aagccttagt atttaattta
     3121 tttgttttct cttatatgta aaaccagcct tattaaccta ggctggtttg atatatattg
     3181 gcttcccttt ttaccttatt tgtcatatta agtcctactt ctagatcctt gtctccctta
     3241 gccattatcc aaaatctatt attacccaaa ttaataactt gtagcctttt atcccgaata
     3301 tctgattttt ccgttgagat tctactggta tcagaagtaa ttatctccat ttccctacaa
     3361 ttatctgcaa agattagatg taatttttca acttccttta taaaaattct ttcagatata
     3421 ggtttattta aaacttttgc tagatattct tgagacctat ataaaatatt ggtgtggtct
     3481 ccctctatta tgatatattg ttggcccaaa tttcccagac tactaaaggt ggtcacagtt
     3541 ccatccccat aatttgtctt attgacccca ataggctctc catctaccca tatgttttgc
     3601 ttacctttat ttaaatctac taaaaactcc atactggttt cttcaccttt accagttatt
     3661 agaaaagtat ttgccctatt tataggtctt tggtttaatt tacttaaaaa aggatttttg
     3721 actgacattt tctcaattgg aatttctttt ctttcaccat cctcttcata atacagataa
     3781 ttgccataat catagctagg caacaggtct tgaactacag gaaataattt tcttaaaggt
     3841 tttaaatacc cttcattatg aaacagttgg aaatatagga taaatccaag ttttatgcca
     3901 ttgtatagaa tatttctttc cacccttgga taaggtagcc tacctccact ccaaaaataa
     3961 taggcattgg cagctccaaa attcggtgtt cctatcatga ttagcttatc tacactggat
     4021 gaactaaaat aggtcatata gctcctacca agcagaccac ctaggctatg gcctataaga
     4081 attactttct tagacccggt tttcttttta acttttttta tgtccttatc taaatactta
     4141 tctgttgatt ttaatattga atttttccaa tcataatagg atacaaagag attttcccct
     4201 tccacatatc ctatagagtt aagaattttt ataaatggtt tgtagatccc ttcagccagg
     4261 ccaaaggaaa agtttccggt acctggtatt acctcatctc ctaaggaacc aaaaagtcct
     4321 ggtataaaaa caataggata atccatctaa atccacctta cctttatacc aggatattca
     4381 atagtatttt tttcgttatc ccatatttat tttttatatc caccgttata agccttttta
     4441 aaactattta gatcaagttt gaaggtcaac ccgtcgtaaa gctttcctct tacctttctc
     4501 agatcctttt tacgcataca ttctttaaat cttaattttt caattagtcc tattacccta
     4561 aaattacctg accaggtctg ggtaaaaata tctttatatc cattatttaa agcatatctt
     4621 atatataaca accaggcctc tgtggcataa ccctttctac gtgaattaag atctgggata
     4681 tttatcccaa ttgttaggta tccttgcccc tcagtatact ggtaatcatt atctatataa
     4741 taggcattaa cccagccaat atgctttttg ctttcatcat taatacaaat ttgaaaacta
     4801 tacctaaatt catttggatc caacttccga gatagtcttt tagtcattaa tcttctgtat
     4861 tcttctaaat taaaaggacc ctcttccatc tcccaagggg catcccattc taaccattca
     4921 gtctctatgg tctcccatct aatcctatct tatatatctt cttttataaa atctctgaga
     4981 aatactttct cccccttaat aagcaccctt ctttcccccc ttggatattg ctgcctatat
     5041 ttaaaacata tatcaatatt atttgattga ctagttgtta aatttaggca gtttttcctc
     5101 acttacataa cttttaggat aatctagccc ctgccatagg tctccaactt cttctgccca
     5161 tacccggtaa tctcccttat cagtctttaa cataaccctt cctaaataag ccaaagtgaa
     5221 ttcttcacca tttttcagat taaatcgaaa gcttataagt tcaccgccaa ccaagtatag
     5281 gtcttttgca tctccctgaa tttttatttg ccaaaaggcc ttcattacct tttcaatatc
     5341 ttccctttct gtaaccaaca taccttctgc ctcaacagga atatcaaacc tatatatttc
     5401 tatattttcc acatcttttg ccttaaaatc cagtatcttg tttttctcct tcctcccaca
     5461 tcctagtaaa acaaaaaata ttgtgataca taaagcaaat acaactaatc ttttcatttg
     5521 accctgtccc ctcttaaaat aaatacaatc tagcctattc aagttaactg gttttaggat
     5581 tatatacata ctatctttta gtaagtattt ttagacttta gtagttttcg tatctgataa
     5641 ccatcactag gatagccacc ataccccatc caggcctttg gatatattat tggaatagag
     5701 gtagatataa acatatatag ttgataactt ctagttaaaa tgatcatctc tcttataagt
     5761 attattccca tcagtgtttt gtcttccata tttctagcaa catacaatag gactatgcca
     5821 aagataagag acaacaatgg cccggctgca agaatcatta ttttttcaat tctagtaagt
     5881 ttggattcat cccaataggt aaatcctata aaggggttta gacccttaag gttaatatct
     5941 aatcttccta agtttaattt ttttaccttc ctcccatttc agcccaatat tatttttaca
     6001 ttttctttag ttaggattaa agcccaaagg gcatgcccaa attcatgaac aattgttgaa
     6061 aatagaaaaa tcacataaaa gaaaacacca gttaagacaa tctctaacgt aaaatttaca
     6121 agtcccatac aattccccct tgaactactt ataaagctgc ggctgtaatt aggctttcat
     6181 cccacatctt ggacaatatt cataatcctt ttcaagagga gacatacagt ttctgcaagt
     6241 ccttctcctg ccataacctg gatccacaac ttccaaatct gattcatcta ttctagttat
     6301 ttctcctctc tcaatggccc tacctaattc cctgtcaatt gtatataggc tgccgcaaca
     6361 tacacttttt ataaaatatt ttttattcca tctaaatagg gggataaaga ataaactaaa
     6421 atatgtatag gtcataaagg cttccaaacg gccgtatgct tcacaagttg gacatactat
     6481 aggctgagaa aaatctaatt ccttttcttt tgttgatata ccaaatacga agaacatctt
     6541 atccttctcc ctttgtttta ttatgatagt aatttcaata tatttattca ttgactcctc
     6601 agatagcagg acccctttcc cactagcttt agacccgatt ctctttctcc ttaatttcat
     6661 tatagcagta attagtccct tttagtagaa aaaaaccacc taaccgatgg tttttctaaa
     6721 tttaattgga ctaggcaaat ttcttactgc ctggtttttc taagggaagc cttccctccc
     6781 tacaaagggg acagtcctta ggagagtagg atacaagttc ttcagataag gcagttacaa
     6841 attttgttcc aaaatctacc tggcctccag ttctatctac taatacagct acccctacca
     6901 gctctccttc ctccctttct acaatatcaa ttacttccct tacagagcca ccagtagtaa
     6961 tcacatcttc cacaaccagt acccgggccc cctttggtat tctaaagcct ctcctaagag
     7021 tcattacatt attttccctt tcagcaaaaa aattttgcac acctagttgc ctggcaaact
     7081 catatgccaa aataattcct ccaattgctg gccctattac aatatctatt ttatctcctt
     7141 taaacctatc tacaatgggt tctactattt ccctagtata atcgggatgc ttcagcaatt
     7201 ttgcacattg catgtaggta tctccatgcc tacctgatgt aaataaaaag tgaccatagg
     7261 ttattacttc caattcttca aatatttctt taatcctttt tttgcttatc acaaatatcc
     7321 ctcctattga taaggtctcc aattctatta gctatataat tcctcttatt tcttctaggg
     7381 acttaattcc cttccttttc ataaagtcct ctattccttt gattatatct aagcttatat
     7441 ctggtttaat aaagttcgcg ctgcctacct gaatagcagt ggctccagcc ataataaatt
     7501 ctagagcatc ctcggcacat gtaatgcctc caattcctat tatgggaacg tcaactacct
     7561 tacttacttc atataccatt cttaaggcta ttggttttat acaagggcct gaaagcccag
     7621 ctgttatgtt gtcaaagata ggttttctct tatatatatc gatggctaag gcattaaaag
     7681 tatttactaa agaaagccca tctgcaccgg cctttacaca agttactgcc atttcttgaa
     7741 tattttccgc attgggagat aatttgacaa taattggttt gcttgtaacc tctcttgtgg
     7801 ccttaactac ttcataggca gggtcacact taattccaaa ggccatgcca cctttcttta
     7861 cattaggaca agaaatatta agctcaataa aatctatacc tgttttatcc aataattggg
     7921 cccctatcac ataatcttct atccttcctc cacctagatt ggccagtata accgtatcct
     7981 tttcctccag ataggctaat tcattattaa taaagccttg tactcctgga ttttgaagac
     8041 ctatactatt cattatccca gcagcagttt catggatcct tctcccctta tttccttccc
     8101 tcatctccaa ggttaggccc ttagaggata tgcc
//
LOCUS       DATGSI010000512         4383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_650571, whole genome shotgun sequence.
ACCESSION   DATGSI010000512 DATGSI010000000
VERSION     DATGSI010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650571"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VK031_07915"
     CDS             complement(<1..1463)
                     /locus_tag="VK031_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HLS53885.1"
                     /translation="MPKLISKDIEKNLDYIRELFEDCYDLIIREIEIGEVLAVKVAFI
                     YIDGLVDKEFLSEYAIGSLLQIEEIKTLTEQGYRTTVFEAIKKEGLATSEIDEVDDLD
                     EGIDLVLSGDTLLLVDGSDKALQIGTGSWPTRGIDEPSTETVIRGPRDGFNETLKFST
                     TLIRRRIKDTNLKIEIYTVGRRSKTNVAVMYIKDIVSNSLIEEVRKRLSDIDIDSVVD
                     SSTLEHLIEDNYLSPFPQIESTERPDSVAASLYEGRVALVVDNSPFVLLVPATIGTFL
                     QSSEDYYTRWLEASVIRLFRILAVFLVLLPPALYVAITAYHPGILPTKLMYFLAASRI
                     RVPFPALVEALLMEMTMELLRESGTRFSGPIGTTIGIVGGLIIGQAAVDAGIVSSLMI
                     IIVAITTIATFVIPSYELSATLRILKFGLIILSGILGLYGITLGVILIGSHLSTLNSF
                     GAPLTSPYSGLGIKEGDLKDTLVKGPTQKLWLRPGFT"
     gene            complement(1518..2363)
                     /gene="ispE"
                     /locus_tag="VK031_07920"
     CDS             complement(1518..2363)
                     /gene="ispE"
                     /locus_tag="VK031_07920"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HLS53886.1"
                     /translation="MEEIIMESHGKVNLILDILYKRPDGYHEINSIMQEISLKDRLKF
                     KEIEKGMIIESNHPTMPRDSNNLIYKVWEKLSSLFGINKGIYVEVKKEIPMAAGLAGG
                     SSNAAATLKALNELWDLKLSQEELIEIGMELGADIPFCLMGGTALVQGIGERLTKISP
                     FSGKYLLLCKPHISISTAQAYRMISPGKDRLDIDKMIKCIEEGNIHCVARRLGNKMED
                     VIIKEHPIIQEIKDIMETYGALGALMSGSGPTVFGLYDDKDKMIFAQKKLLEKMNKVY
                     ICETI"
     gene            complement(2427..3962)
                     /locus_tag="VK031_07925"
     CDS             complement(2427..3962)
                     /locus_tag="VK031_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOCS domain-containing protein"
                     /protein_id="HLS53887.1"
                     /translation="MSIEIIKENINIEELKGQSEVQTLVETEIYLNTIKENIKEIIWT
                     EGRIDITNTMIIKDKILVNGLVKFNLVYKSEEENIHSLDSIKDFNVEMFIKGLREDMI
                     PTVESNIEFIEEELKENKLELRAVINLNGEVVETKTLEVIQGIEGRDDLEVLSENIKY
                     EEVYGRGQSQLDIREMFHIGEGQASIERVIKFFVETREIESAVTEDRIILSGEALITL
                     IYVADNELYSIKETIPFNHFLDLPEVEEGAQCEIKLSVIEAIYDVMEDEVGEQRLVDV
                     EIKLGVSGRAYDENTKDLIIDAYSTEEEIILEKEEINIPEKIDKIEYNERVNLEAPIN
                     AIDILDIRREHSIIDKKITEEGVIIEGAINVVVYYIDRIGGEIANFIELYPYTFTIPY
                     EGEDRDLSIVVTTRLADIDYSIKRDAVLIENNVYHSIVLSKKRWIYGIRDIQGTGNII
                     DKSNMPSITIYIVQEGDLLWDIAKRYNTTAEDILSSNNLEVDYELQVGDKIIIEKKLD
                     LDF"
     gene            complement(4079..4339)
                     /locus_tag="VK031_07930"
     CDS             complement(4079..4339)
                     /locus_tag="VK031_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HLS53888.1"
                     /translation="MDSLAKIRKDLEGCIGKTVILRANKGRKRIVVREGILESAYPNL
                     FVVKISNEYDSTRRVSYTYSDVLTSTVEVTVCDGQDRQTKIS"
BASE COUNT         1249 a          948 c          588 g         1598 t
ORIGIN      
        1 aatgtaaacc ctggccttag ccataatttt tgagttggcc ccttaacaag tgtatctttt
       61 aagtcccctt cctttatccc caatccagaa tagggagatg ttaggggtgc tccaaaacta
      121 tttaaggtag ataagtgaga acctatcaaa ataaccccta aagtaattcc atataaacct
      181 aatatgcctg ataatataat taaaccaaat tttaagatcc ttaaggtggc ggaaagttca
      241 taagaaggga ttacaaaagt tgctatagtg gttatagcta caattatgat cattaacgaa
      301 cttacaatac ctgcatctac tgctgcctgc ccaataatta gacctcctac tatacctatg
      361 gtggttccaa ttggccctga aaatctagtt ccagactctc ttaataattc catagtcatc
      421 tccatcaaaa gtgcctcaac taaggctgga aaagggacac ggattctgct ggctgccaaa
      481 aaatacatta acttagttgg caatatacct ggatgatagg ctgttatggc tacatataag
      541 gctggaggta aaagaactaa aaaaactgcc aatattctaa acaacctaat aaccgaagct
      601 tccagccatc tggtataata atcctctgaa gattgtagaa aagtacctat tgtagctggt
      661 actaacaata caaaaggtga attatctacc accagggcca cccttccttc atatagagaa
      721 gctgccacac tatccggcct ttctgtactt tcaatttgag gaaaggggga tagataatta
      781 tcctcaatta aatgctctaa ggttgaacta tctaccactg agtctatgtc tatgtcagat
      841 aacctttttc taacttcctc tattaaacta ttagacacga tatctttaat atacattact
      901 gctacattag ttttagacct tctacctact gtatagattt caattttgag attagtatcc
      961 tttatccttc ttctaattaa tgtagtgcta aactttaatg tttcattaaa gccatcccta
     1021 ggacctctta taactgtttc tgtactaggt tcatctatac ctcttgtggg ccaggaccca
     1081 gttccaattt gtaatgcctt atctgagccg tctaccaata agagggtatc tccagataag
     1141 acaagatcaa ttccttcatc caaatcatct acctcatcta tctcagaagt agccaagcct
     1201 tcttttttta tggcttcaaa aactgtagtc ctataaccct gttctgtaag tgtttttatt
     1261 tcttctatct gtaaaagaga gccaattgca tactcagata aaaattcttt atccaccagt
     1321 ccatctatgt atataaaagc aacctttaca gctagaactt ctcctatttc aatttctctt
     1381 ataatcaaat cataacaatc ttcaaataat tccctaatat agtccagatt tttttctata
     1441 tctttagata ttaatttggg cataaaaata ctcacctctg taaactatat agtttccaaa
     1501 aggtgagact attatactta tatggtttca catatatata ctttattcat cttttcaagc
     1561 agcttcttct gcgcaaagat catcttgtcc ttatcatcat ataaaccaaa aacagtaggc
     1621 ccgcttccgc tcatcagggc ccctagggct ccataggtct ccattatatc tttaatttcc
     1681 tggattatgg gatgttcttt aatgattaca tcctccatct tattgcccag ccttctagcc
     1741 acacaatgga tatttccctc ttctatacat ttaatcatct tatcaatatc cagcctatct
     1801 ttgccagggc taatcatcct gtaggcttgg gcagtagata ttgatatatg gggcttacat
     1861 aataataggt atttgcctga gaagggactt attttagtta gtctttctcc tataccttgg
     1921 actaaagccg tccctcccat aaggcaaaag ggtatatctg ccccaagctc catgcctatc
     1981 tctattaact cctcctggct tagttttaaa tcccaaagtt catttagtgc ctttagggtg
     2041 gcagcagcat tggaactacc acctgctaaa cctgcagcca tagggatttc cttttttact
     2101 tccacataaa tccccttatt tataccaaat aggctactta acttttccca aaccttgtat
     2161 attaaattat tagagtccct aggcatggta gggtggtttg attctattat catacccttt
     2221 tctatttcct taaacttcag cctatcttta aggctaattt cctgcataat actattgatt
     2281 tcatgataac cgtcaggcct tttatatagg atgtctaata ttagatttac cttgccatgt
     2341 gactccatta ttatttcttc catgtctatc cccttatatc agttaagata gggttattat
     2401 atcataagaa tagaaatatt tgtaatttaa aagtccaggt ccaatttctt ttctattatt
     2461 attttatctc ctacctggag ttcataatca acctctaagt tgttagaact tagtatatcc
     2521 tcagcagttg tgttatatct tttggctatg tcccataaaa gatctccctc ttggactatg
     2581 taaatagtaa tactgggcat attactttta tctattatat ttcctgtacc ttgtatatct
     2641 ctaatgccgt aaatccatct ttttttgctt aacacaatac tatggtaaac attattttct
     2701 atcaagactg catctctctt tatagagtag tctatatctg ctagcctggt ggttacaact
     2761 atactaaggt ctctatcctc tccttcatag ggtatggtaa aagtatatgg atatagctca
     2821 ataaagttgg ctatctctcc ccctatccta tctatgtaat aaaccactac atttatggct
     2881 ccttctatta tgactccctc ttcagtaatc tttttatcta taatactatg ttctcttcta
     2941 atatctagga tatcaattgc attaataggg gcctccaggt taaccctttc gttgtactct
     3001 atcttatcta tcttttctgg tatgtttatt tcttctttct ctagaataat ttcctcctct
     3061 gtggaataag catctatgat taaatctttt gtattttcat cataggccct accagaaact
     3121 cctagtttaa tttccacatc taccaatctt tgctctccta cctcatcctc cattacatcg
     3181 tatatggcct ctattacact taatttaatc tcacactggg ccccttcttc tacctctggc
     3241 aggtctagaa agtgattaaa gggtatggtc tctttaatcg agtaaagctc attatcagct
     3301 acataaataa gggtaatcag ggcttcacca gataagatta ttctatcttc tgtaacagca
     3361 gattctattt ctctagtttc tacaaagaat tttataactc tttctatact ggcttgacct
     3421 tcccctatat gaaacatttc cctaatatct aactgggact ggcctcggcc ataaacttcc
     3481 tcatatttta tattttcgct taatacctct agatcatctc taccctctat tccctgtatt
     3541 acctctaaag ttttagtttc tactacttct ccattaagat taataacagc ccttaattct
     3601 aacttatttt cttttagttc ttcctcaata aattctatat tgctttcaac tgtaggaatc
     3661 atatcctctc ttagcccctt gataaacatt tctacattaa agtccttaat actatctaga
     3721 gagtgtatgt tttcttcctc tgatttatag accaggttga attttactaa cccatttacc
     3781 agaatcttat cttttattat catggtatta gttatgtcaa ttcttccctc agtccaaatt
     3841 atctccttta tattttcctt tattgtattt agatagatct ctgtttctac caaggtttgt
     3901 acttctgatt gaccttttag ttcctcaatg ttaatatttt cctttattat ttcaatggac
     3961 atattctcac ccctataaat tacttttaat cctatagaaa tatatatgag gtgatgtcct
     4021 attttatgta taaataaaaa aagtactatc ttgaaaccaa aatagtactt cctctctttt
     4081 atgaaatttt cgtttgtcta tcttgaccgt cgcagactgt tacttcaact gtagaagtaa
     4141 gtacgtctga ataggtgtag gaaactcttc ttgtgctatc atattcatta gatatcttta
     4201 ccacaaataa attgggataa gctgattcta gtataccttc tcttactaca attctttttc
     4261 tacctttgtt tgctcgtaaa attactgttt tgccaataca tccttccagg tccttacgaa
     4321 ttttagctag actatccata tactaggcta aaccaccttc cattggattc tctcctgtgt
     4381 gta
//
LOCUS       DATGSI010000513         1088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_697226, whole genome shotgun sequence.
ACCESSION   DATGSI010000513 DATGSI010000000
VERSION     DATGSI010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697226"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VK031_07935"
     CDS             complement(<1..992)
                     /locus_tag="VK031_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53889.1"
                     /translation="MTSVGGSVGGENWCVYEDLDPYCDTAAIMSYGMAWAGSAPGPVS
                     PRDWLEGIYNYASKVMTPEKIFLGLPAYGWNWRIHDSPANLGNVYRGVSNTYYAAQLW
                     MTGGYNFTDDGPPQPMIPIIAYWDDYDKVPWALPHVYDYMEGPDATGRTYPLLGESYN
                     RRRYLTAYSKEQEVEFGTVYIDRDGGSPDTYSEGVILSSSSATLGEDGEAEYEFNIAN
                     SGTYDVAVRILFPFWDKNSINISLDGVSKNFTENRLWWPYWRSTCWLSLSSSVFLSAG
                     THTVSITTGVPGVLCYGFRVCSNFKEQPFAGEAEFTLSPRKFKDVNGVMAEPDRG"
BASE COUNT          347 a          253 c          186 g          302 t
ORIGIN      
        1 cctctatctg gctcagccat tacaccatta acgtccttaa actttctagg tgataaggta
       61 aactcagcct caccagcgaa gggctgttct ttaaaattag agcagacacg aaatccataa
      121 cacagtactc caggaacccc agtagttatg cttacagtat gagtaccagc tgaaagaaag
      181 acactagatg ataaagagag ccaacaggta gatcgccaat atggccacca taatctattt
      241 tcagtaaagt ttttacttac tccatcaagg gatatattga tgctattctt atcccaaaag
      301 ggaaataaga tgcgaacagc cacatcatat gttccagagt tagcaatatt aaattcatat
      361 tcagcctcac catcttcacc aagggttgct gagctagagg atagaatgac tccttctgaa
      421 taggtatcag gactcccacc atcacggtca atatagactg taccaaattc tacttcttgc
      481 tctttactat aagcagttaa atatctacgt cggttataac tttctccaag gaggggatat
      541 gttctacctg ttgcatctgg cccttccata taatcgtata catggggtaa agcccatggc
      601 actttatcat aatcatccca ataagcgata attggaatca tgggctgtgg tggaccatcg
      661 tctgtaaaat tatatcctcc agtcatccaa agctgtgccg cataataggt attagaaact
      721 ccacgataaa cattacctaa atttgcaggt gaatcatgaa ttcgccagtt ccaaccatat
      781 gcaggtagcc ctaaaaatat cttttctggt gtcataacct tagatgcata attatatatg
      841 ccttctagcc agtccctagg ggatacaggg ccaggagcag aacctgccca tgccatacca
      901 taactcataa tggctgcagt atcacaatac ggatctaagt cctcataaac acaccagttc
      961 tcgccaccaa cagaaccccc tacacttgtc attcctggca agcaaatatt aactagtttt
     1021 gatgaattat aacctttaac agtattataa atatcacgaa atagaatatt ggcagcatct
     1081 ttattttc
//
LOCUS       DATGSI010000514         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_266981, whole genome shotgun sequence.
ACCESSION   DATGSI010000514 DATGSI010000000
VERSION     DATGSI010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266981"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VK031_07940"
     CDS             <1..359
                     /locus_tag="VK031_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HLS53890.1"
                     /translation="KKKALLDNWFRERANLIFDESLNRMHRLVEKYNIEKPEMKIRKM
                     KSRWGTCYVNRKIIILNLELIHAPKYCIDYVVLHELVHFKHKNHNKDFYNLLTTLMPD
                     WKNRKKILDQVIIKKI"
     gene            complement(386..1816)
                     /locus_tag="VK031_07945"
     CDS             complement(386..1816)
                     /locus_tag="VK031_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HLS53891.1"
                     /translation="MNKIESKEREQWGTRIGFILAAAGSAVGLGNIWRFPYMTGENGG
                     SAFILIYLIFVIIICLSIMIAEFAIGRRSRLSAVGAYKSISPNWTFAGVLGVLCAFLI
                     MGFYPVVGGWSLAYIFKSISGLLSSTAISDAFGDFIGNPIEPIIWMAIYLSFNLFIVA
                     KGISEGIEKAGRILMPTLFILLFIIIGRSVTLPGSSAGMDFLLNPDFSKVTGQTFLAA
                     LGQAFFSLSLGMGCMMTYGSYLSSKENIPKNALLITMLDTVCAILAGLAIFPALFAFG
                     LEPTEGPGLVFVVVPTIFSKMGSIGVILSIIFFIALTVAALTSSVSLLEVVVAYLIDE
                     KKLARKIAVGISGGVMVITSMLSSLSMGILQDFHVLGLGVGFFDFFDILTDKIFLAIG
                     GMLLAIFVGWNVNKEDLKDELTNGGEIDFPLFNLWYILMKWIIPIAIIIVASMGMVSM
                     IKNGLLPLMLFGIGIIIASAIFSKKL"
     gene            complement(1989..2423)
                     /locus_tag="VK031_07950"
     CDS             complement(1989..2423)
                     /locus_tag="VK031_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLS53892.1"
                     /translation="MDIVMKEIKYGSPEYKETIQLRNEVFRKPWGLDIKDDDLSPDKE
                     MDIYGAYLDGKLIGTVFLAHHEEGVARVRNVAIYKEYRGKGLGKYLMDFIEDKAREKG
                     YRKMYLMGRTNVEKFYKKLGYKTMGKPYDYRTIPHVDMIKDL"
     gene            complement(2461..>2815)
                     /locus_tag="VK031_07955"
     CDS             complement(2461..>2815)
                     /locus_tag="VK031_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53893.1"
                     /translation="IDQLSIALLVLSIVLAIVFAFVPYEFIRLLYIGLLVYTYFRIFS
                     RNINQRYKENYAFLKFISPVRKKWNQILAQFKGRKTHKFYKCSFCSQMIRVPKGKGKI
                     KITCPKCRNTMIRRT"
BASE COUNT          996 a          563 c          409 g          847 t
ORIGIN      
        1 aaaaaaagaa ggccttatta gacaattggt ttcgagagag ggccaatctc atatttgatg
       61 aatccttaaa taggatgcat aggctggttg aaaaatataa tattgaaaag ccagaaatga
      121 aaataaggaa aatgaaatct agatggggaa cctgttatgt caataggaag attattatct
      181 taaacctaga attaatccat gcacctaaat actgtataga ttatgtggtc ttgcatgaat
      241 tagttcactt taagcataaa aaccataata aagacttcta caatctccta accaccttga
      301 tgccagattg gaaaaataga aagaaaatat tagaccaagt aatcataaaa aaaatataaa
      361 gggtccaatt taggaccctt tatctctaca gttttttaga aaagatagct gatgcaatta
      421 taatcccaat cccaaacagc atcagtggca ataacccatt ctttatcata gataccatcc
      481 ccatgctggc aacaattatt atggctatag gaattatcca tttcatcaag atataccaaa
      541 ggttaaataa tggaaaatct atttcaccac cattagttaa ctcatctttt aggtcctctt
      601 tatttacatt ccaacctaca aatatggcta aaagcatacc tcctatagct aggaatatct
      661 tgtcagtcag aatatcaaaa aaatcaaaaa aacctacgcc taagcccaag acatgaaagt
      721 cttgcaatat tcccatggat agagaagata gcatactggt tataaccata actcctccag
      781 aaataccaac tgcaattttt ctagccagct ttttctcatc aattagatag gctactacaa
      841 cttctaaaag ggatacagat gaagttaagg ctgccacagt taaggcaatg aaaaatataa
      901 tagacagtat aactccaata cttcccatct tactaaatat ggtaggtact actacaaata
      961 caaggcctgg tccttcagta ggctctaacc caaaggcaaa gagagccggg aaaatggcta
     1021 aacctgctaa gatggcacaa actgtatcca acatggtaat tagtagggcg tttttaggga
     1081 tattttcctt actactaagg tagcttccat aagtcatcat acaacccatt cctaaactta
     1141 aggaaaaaaa agcctggcct aaggcagcta aaaaagtttg acctgtaacc ttagaaaaat
     1201 ctggatttaa taaaaagtcc atgccagcag aggacccagg tagggtgaca ctacggccaa
     1261 taataatgaa taataatatg aataatgtgg gcatcaaaat tcttcctgcc ttttctattc
     1321 cctcagatat tccttttgcc actataaaaa gattaaagga caagtaaata gccatccata
     1381 taataggttc tattgggtta cctataaaat caccgaaagc atcagaaata gcagtagacg
     1441 ataataggcc ggatatagac ttaaaaatat aggcaaggct ccatccacct actacaggat
     1501 aaaagcccat tattagaaag gcacataaaa cccctaatac tcctgcaaag gtccagttag
     1561 gactaatact tttataggca ccaacggctg aaagtctact cctcctacct atggcaaact
     1621 ctgcaatcat aatacttaag caaattatta taacaaaaat taaataaata agtataaatg
     1681 cacttcctcc attttcgcca gtcatatagg gaaatctcca tatattacct agacctacag
     1741 cagatccagc ggctgctaaa atgaagccaa tcctagttcc ccattgttct ctttcttttg
     1801 attcaatttt attcattttt atcctccttc ctaattgtaa ctttgtataa tggtataatt
     1861 ccttaaatta tacaacatgc tttaacataa tgcaacacaa aagtgataaa acgaaaaagt
     1921 tttaggagga tattctaaga aaaaaccata gcagtatatg ctatggttaa atttttccta
     1981 tgaggagctt ataaatcctt gatcatatct acatgaggaa tagtcctata atcataaggt
     2041 ttacccatgg tcttatatcc taatttttta taaaattttt caacgttggt tcgccccatc
     2101 aggtacattt tcctataacc tttttcccta gctttatctt caataaagtc cattaggtat
     2161 ttgcccaaac ccttgcccct atattcttta taaatggcca cattccttac cctggctact
     2221 ccttcttcat gatgggccaa aaatacagtc cctatgagtt tgccatctaa gtaggcacca
     2281 taaatgtcca tttccttgtc agggcttagg tcatcatctt taatgtccag tccccaaggc
     2341 tttctaaaga cctcattcct aagctgtata gtctctttat attctggact accatatttg
     2401 atctctttca taactatatc cataaaaatc cccctttatt tagacactta tcttcaaata
     2461 ctatgttctt ctaatcatag tatttctaca cttaggacaa gttattttaa ttttaccctt
     2521 accctttggg acacgaatca tttggctgca aaaggagcat ttataaaact tatgggtctt
     2581 tctcccctta aattgagcca aaatttggtt ccatttcttt cttactggac taataaattt
     2641 taagaaggca taattttcct tatacctttg attaatattt ctagaaaaga tcctaaagta
     2701 tgtatataca agcaaaccta tgtaaagaag ccttataaat tcatatggga caaaagcaaa
     2761 tacaatggct aatacaatag ataagactaa aagagcaatg gaaagttgat ctatc
//
LOCUS       DATGSI010000515         1448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_650821, whole genome shotgun sequence.
ACCESSION   DATGSI010000515 DATGSI010000000
VERSION     DATGSI010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650821"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..816)
                     /gene="rpsB"
                     /locus_tag="VK031_07960"
     CDS             complement(37..816)
                     /gene="rpsB"
                     /locus_tag="VK031_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HLS53894.1"
                     /translation="MSVVTMKSLLEAGVHFGHQTRRWNPKMAPYIFTERNGIYIIDLQ
                     KTVKMVDEAYNFVREIAAEGGEILFVGTKKQAQEAIETEAKRCGMHYVSQRWLGGMLT
                     NYRTIRKRIDRLHELREMEEEGIFDVLPKKEVIQLNHEADRLEKFLGGIKNMDRLPDA
                     IFVVDPRKERIAVREARILGIPVIAIVDTNCDPDEIDYIIPGNDDAIRAVKLLTETMA
                     NAVLEGKQGEQMEMEEEVQEETETVEEVEAEEKVETEVEVE"
     gene            complement(992..>1448)
                     /gene="codY"
                     /locus_tag="VK031_07965"
     CDS             complement(992..>1448)
                     /gene="codY"
                     /locus_tag="VK031_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HLS53895.1"
                     /translation="GKIATFIPINSAGKRLGTLVLARFGEEFTEEDLILAEYSATVVG
                     MEILRSKNEEIEEEARKKSIVQMAIGTLSYSELEAMEHIFNELDGTEGLLVASKIADR
                     VGITRSVIVNALRKFESAGVIESRSLGMKGTHIKILNDKLLEGLKKARS"
BASE COUNT          372 a          348 c          184 g          544 t
ORIGIN      
        1 aaaaggtgat taaccttaga acccttacct tctaaattat tctacttcaa cttcagtttc
       61 aactttttct tcagcttcaa cttcttctac agtttcagtt tcctcttgga cctcttcttc
      121 catctccatt tgttcaccct gttttccttc caaaacagca ttggccattg tttctgttag
      181 taattttaca gctcttatgg cgtcatcgtt tcctggaatg atataatcaa tttcatcagg
      241 gtcacaatta gtatctacta ttgctataac cggtatacct aaaattctag cctctcttac
      301 agcaatcctt tcctttctag gatcaactac aaatattgca tctggaagtc ggtccatatt
      361 tttaattcca cctaaaaact tctccagtct atcagcttcg tgatttaact gaattacttc
      421 tttcttaggt aagacatcaa atattccttc ttcttccatt tcccttagct catgtaatct
      481 atcaattctt tttctaatag ttctatagtt ggtaagcatc cctccaagcc atctttggct
      541 tacatagtgc ataccacacc ttttggcttc agtttctata gcctcttgag cttgcttttt
      601 agttcctaca aataggattt ctccaccttc tgcggcaatt tccctcacaa agttataggc
      661 ctcatccacc atcttaactg ttttttgtag gtcaataata tagataccat ttctttctgt
      721 aaagatatac ggagccatct tagggttcca tcttctagtt tgatgtccaa aatgaacacc
      781 agcttctaat aagcttttca tcgtaactac tgacatactt tttcacctcc aagttttatt
      841 cctccatttt cttcatctac agtgggaccc taattgggac cgcccactat atcggaaaat
      901 gtgcgtaatc actatagtag tataacataa agttattttt taggcaataa attttagaaa
      961 atatattcac attcttacca tgacttttaa attagcttct tgccttctta agtccttcta
     1021 gcaacttatc atttaatatt tttatatggg ttcccttcat ccctagagat ctagactcaa
     1081 tgacacctgc actttcaaac tttctaagtg catttactat aactgatctt gtaatgccaa
     1141 ccctgtctgc tatcttgctg gcaaccagaa gtccctctgt tccatccagt tcattgaaaa
     1201 tatgttccat tgcctccaac tcagagtagg ataaggttcc aatggccatt tggactatag
     1261 atttttttct tgcctcttct tctatctctt cattcttaga ccttaatatt tccatgccaa
     1321 ctactgttgc actgtattcg gccagtatta gatcctcttc tgtaaattct tcaccaaatc
     1381 tagccaaaac taaagttccc agccttttgc ctgcgctgtt tataggtatg aatgtagcta
     1441 ttttacct
//
LOCUS       DATGSI010000516         1512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_178515, whole genome shotgun sequence.
ACCESSION   DATGSI010000516 DATGSI010000000
VERSION     DATGSI010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178515"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..277
                     /locus_tag="VK031_07970"
     CDS             104..277
                     /locus_tag="VK031_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53896.1"
                     /translation="MNNQDPVGVGQIQIPLPEYPEWILDYLAPKLLEYAKKVQRDAIK
                     YFIENMRGDNGNY"
     gene            264..761
                     /locus_tag="VK031_07975"
     CDS             264..761
                     /locus_tag="VK031_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53897.1"
                     /translation="MEIIKVFGGVVEALQTYNRDVDTLMELGVEVGESTSPKMIESDL
                     LTIKYFPCTGDIPDTLKGLSCDKLIFCGSVDPHLKSIIESSVKDRGKEKKQSEDIRNK
                     REELENKIKSLKEDISSLQDMCLHPYLKKEYSSSGGGWDDSTNDMSWIDFSCPDCGKR
                     WSEDY"
     gene            792..1013
                     /locus_tag="VK031_07980"
     CDS             792..1013
                     /locus_tag="VK031_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53898.1"
                     /translation="MKENLFSVGKTVYNALNVKPIRAYSTATEDRVRIEFIDGDRVEV
                     GTFWPSNRDNYLEIYRKFYICPTTHEVVL"
     gene            1028..1213
                     /locus_tag="VK031_07985"
     CDS             1028..1213
                     /locus_tag="VK031_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53899.1"
                     /translation="MFRLRERARIRRQIPTRKSVQEGKPDCISDLLEEAAEEIELLRG
                     TLKYFVRINSEQSLGER"
     gene            1216..1404
                     /locus_tag="VK031_07990"
     CDS             1216..1404
                     /locus_tag="VK031_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53900.1"
                     /translation="MKEVLLKLEGVIGEVEECPKYLLGIKMDIQNHLLRIELGEKLEL
                     IFPTTSLYGEGVVLSMNG"
BASE COUNT          475 a          236 c          393 g          408 t
ORIGIN      
        1 gtctgataaa ggtggctccg ttttttggtt tttcgtatct tgctgctctg ctgtgacagt
       61 atttaacctt atatatgttt atcttgttct aggaggatga agagtgaaca atcaagatcc
      121 cgtaggggtg ggtcaaatcc aaatcccctt gccagaatat cctgaatgga ttcttgacta
      181 cctagctcct aaacttttgg agtatgcaaa gaaagtacaa agagatgcaa taaaatattt
      241 tattgaaaac atgaggggag acaatggaaa ttattaaagt atttggtggt gtggtggagg
      301 ctctccaaac atacaacagg gatgtggaca cattaatgga attgggagtg gaggtagggg
      361 agagtacctc tccaaagatg atagaaagtg atctgctgac tattaaatac ttcccctgta
      421 caggagacat tccagacacc ttgaaaggac tttcttgcga caaacttata ttctgtggta
      481 gtgtagaccc acatctgaaa tctattatcg aatcttcagt aaaggatagg ggtaaagaga
      541 agaagcagtc tgaggacatc agaaataaga gggaagaatt agagaacaag ataaagagcc
      601 ttaaagaaga catttcatcc cttcaggata tgtgcctcca cccctacctt aagaaggagt
      661 atagtagtag tgggggaggg tgggacgaca gcactaacga tatgtcttgg attgacttct
      721 cctgtcctga ctgtggtaaa aggtggtctg aggattatta agttccccca cttagttagg
      781 gagaatcata aatgaaagaa aacttatttt cagtaggtaa gactgtatat aatgccctta
      841 acgtgaagcc tataagggcc tactccactg ctacagagga tagggttagg atagagttta
      901 ttgatgggga tagggtggag gtagggactt tctggccctc aaacagagat aattatctag
      961 aaatctatag gaagttttac atttgcccaa caacacatga ggtcgtgcta tgacagaaga
     1021 agatttagtg tttcgattaa gagagagggc cagaatacgt agacagatcc ctactcgtaa
     1081 atctgtacaa gagggaaagc cagattgtat ttccgatctt ctcgaagagg cggcagaaga
     1141 aattgaattg ttaaggggca ctctgaaata ttttgttcgg attaattccg aacaaagctt
     1201 gggggagagg tgagaatgaa agaagtattg ctgaaactgg aaggggtgat aggtgaagtg
     1261 gaggagtgtc caaaatatct tctgggcata aagatggaca tccaaaacca tttgctgagg
     1321 attgaattag gagagaaact tgagttaatc ttcccgacca cctctcttta tggggagggt
     1381 gttgttctat caatgaatgg atgaaaatag ttaagtttga agaaggttct ggaagagaga
     1441 ttagttggcc tgatgatggg aagcagcccg aagatgggtg gtatttacaa ttggggtttc
     1501 ctacaggagc ct
//
LOCUS       DATGSI010000517         3037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_604310, whole genome shotgun sequence.
ACCESSION   DATGSI010000517 DATGSI010000000
VERSION     DATGSI010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604310"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VK031_07995"
     CDS             <1..367
                     /locus_tag="VK031_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53901.1"
                     /translation="MNDIYRAERAIKDILGEEFDSRIIRFPGGSFETRKKPMRKAIVR
                     AGYQYFDWNALNGDAEGKYKDKRQLLNRLKETTKDKENIVVLMHDTDAKQSTVEFLRD
                     GIEYLISQGYEFAVLKKNN"
     gene            383..1075
                     /locus_tag="VK031_08000"
     CDS             383..1075
                     /locus_tag="VK031_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLS53902.1"
                     /translation="MTRTILIVEDEDSIRKFIRINLEKAGFTVLEAETGEKGIDIARS
                     ESIDIVVLDIMLPGIDGFETCKVLREEFPELGIIMLTAKSQDVDKIMGLEYGTDDYMT
                     KPFNPMELVLRVKSLARRMDIPDEPEESILSFGPFRIDKYSREFYKEGTEMDLTPTEY
                     QIVKLFMENPGRAFKRDEILNLVWGEDFVGDSKIVDVNIRRLRSKIEDDPSNPYFIET
                     VWGLGYRWRNKE"
     gene            1084..2484
                     /locus_tag="VK031_08005"
     CDS             1084..2484
                     /locus_tag="VK031_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLS53903.1"
                     /translation="MKKSIKTRLTRSFMILIIITVLILLIILTNAIKDYYYKSLEDIM
                     TSQMEYSIDLYSRYYSSLSMNEILVDDIDIFGRHDNLQFQLLSLDGELLMDSLGAQTD
                     SHINTSDVADAIRDGKGVWIGNVDYDSYPVMAVSMPLKGHGEAIGIVRFVSSLKETNK
                     IIQRITMLLVSLGVAVVVVSGIVGIFLSNSIVKPLVELTRVAEKMADGQLRVRSTLEL
                     DDEIGRLSNTLNYMADEVLKREEIKNDFISSVSHELRTPLTSIKGWAMTLQADDIRDE
                     NLTKDGLKIIENESDRLAQMVEELLDFSRFVSGRITLEKDIFDIGSALETVSRQYMPR
                     AKEKKIDFSLHVNEDVDYFLGDENRIKQVLINLLDNAFKFTNEGGKVKLSAYKTEKNL
                     VLQVKDTGIGIPDDDLPHIKEKFYKGKNSKSQSGIGLSICDEIVKLHDGALKIKSKLN
                     YGTTVEAILPIKEEEV"
     gene            2481..>3037
                     /locus_tag="VK031_08010"
     CDS             2481..>3037
                     /locus_tag="VK031_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53904.1"
                     /translation="MKKLLKVFLILSALLTMTACNISETDINERLSAPSNQLPPIEGK
                     WVIKEHIDLDQKPGEDGEERFIGREAMFHREAVILASNYTKEPSYKIKNVYTRDYFIY
                     KYKLRSSTLDIENEKVQVITVLDDNQYFAEFVKVSEEDLLVYLNNSFYRMEKVSEQVS
                     LNEINRYIEIEKAVVREFDSYEDENL"
BASE COUNT         1120 a          367 c          670 g          880 t
ORIGIN      
        1 tatgaatgat atatataggg ccgaaagagc tataaaagac atattaggtg aggaatttga
       61 ttcaagaata atcagatttc caggcggttc ttttgaaacc aggaaaaagc caatgagaaa
      121 ggcaatagtt agggctggct atcaatactt tgactggaat gcacttaatg gtgatgctga
      181 aggaaaatat aaggataaaa ggcaactatt aaatagatta aaggaaacaa ctaaggataa
      241 agaaaatata gtagttttga tgcatgatac tgatgccaaa caatccactg ttgaattttt
      301 aagagatggg atcgaatatt taatatccca gggatatgaa tttgccgtat tgaaaaaaaa
      361 taattaagag ggtagtgatt tcatgactag gacgatctta attgtggaag atgaagattc
      421 tattagaaag tttattagga tcaatttgga aaaggctgga tttacggttt tagaggctga
      481 gaccggtgaa aagggtatag acatagccag gagtgaaagt atagacatag ttgtattaga
      541 tattatgtta cctggtatag atgggtttga gacttgtaag gtcttaagag aagaatttcc
      601 tgaactaggt ataattatgc ttacagctaa aagccaggac gtagacaaga ttatgggact
      661 agaatacgga acagatgact atatgaccaa gccctttaat ccaatggaat tagttttaag
      721 agtaaagtct ttggccagaa ggatggatat tccagatgaa cctgaagaat caatacttag
      781 ctttggtccc tttaggatag ataagtattc cagagagttt tataaagagg gtaccgagat
      841 ggacttaact ccaacagaat atcagatagt taaattgttt atggaaaatc ccggtagggc
      901 ctttaaaagg gatgaaatct taaacctagt ctggggcgaa gattttgtag gagattcaaa
      961 aattgtggat gttaacatta ggcggcttag atctaaaata gaggatgatc caagtaatcc
     1021 ttattttata gagactgtat ggggacttgg atatagatgg aggaataaag aatagatgtg
     1081 aagatgaaaa aaagtataaa aactaggcta actagaagct ttatgattct aattattatt
     1141 actgtattaa tattattgat tatacttaca aatgctatta aagattatta ctataaaagc
     1201 ctggaagata taatgaccag ccagatggaa tattctatag atttatattc tagatattac
     1261 agttctctaa gtatgaatga gattttagtt gatgatatag atatatttgg aagacatgat
     1321 aatcttcagt ttcagctatt atctttggat ggagaattgt taatggactc attaggagcc
     1381 cagactgact cacatataaa tactagcgat gtggccgatg ccataagaga tggcaaaggt
     1441 gtatggattg gtaatgttga ttacgattct tatcctgtaa tggcagtatc tatgcctttg
     1501 aagggacatg gtgaggccat agggatagtc aggtttgtat cttcattaaa agaaacaaat
     1561 aaaataattc agagaataac catgttatta gtatccttgg gagttgccgt tgtagttgtt
     1621 tcagggattg tgggtatatt tttatctaat tctattgtaa agcctttagt tgaacttact
     1681 agggtggcgg aaaaaatggc agatggccaa ttaagggtaa gaagtacatt agaattagat
     1741 gatgagatag gcagactgtc taatacccta aattatatgg cagatgaggt tttaaaaagg
     1801 gaagaaatta aaaatgactt tatatcatca gtttcccatg aattaagaac cccactaaca
     1861 tctattaaag ggtgggccat gaccttgcag gcagatgata taagggatga aaatctaact
     1921 aaggatggac taaaaattat tgaaaatgaa agtgatagat tggcacaaat ggtagaggaa
     1981 cttttagatt tctctagatt tgtttcagga agaataacct tagaaaagga tatttttgat
     2041 ataggttcag ccctagaaac tgtatccagg caatacatgc ctagggctaa ggaaaagaag
     2101 atagactttt ccttgcatgt aaatgaggat gtggactact ttctaggtga tgaaaaccga
     2161 ataaagcagg tattgattaa tcttttagat aatgccttta aatttactaa tgaaggcgga
     2221 aaagtcaagc taagtgctta taaaacagaa aaaaatctag tattacaggt aaaggatact
     2281 ggcataggca tccctgatga tgatttacca catataaaag aaaagtttta taagggtaaa
     2341 aacagtaagt cacagagcgg aataggccta tccatatgtg atgagatagt taagttgcat
     2401 gatggagcct taaaaattaa gtccaagctt aattatggca ctactgtaga ggccatccta
     2461 cctattaagg aggaagaagt atgaaaaaac tgcttaaggt cttcctaata ttgtcagctt
     2521 tattgaccat gactgcatgt aatattagtg aaaccgatat taatgaaagg ctaagtgcac
     2581 caagcaacca gttaccccca atagagggca aatgggtaat aaaagaacat atagatttag
     2641 atcaaaagcc tggggaagat ggtgaggaaa gatttatagg aagggaagcc atgttccata
     2701 gggaggctgt aattttagcc agtaattata ctaaagagcc atcatacaag ataaaaaatg
     2761 tatacactag agattatttt atctacaaat ataaactaag atcaagtaca ttggacatag
     2821 aaaatgaaaa ggtacaggtt ataacagtgt tagatgataa tcaatacttt gctgaatttg
     2881 taaaggtgag tgaagaggat ctattagtat atttaaataa cagtttttac aggatggaaa
     2941 aggtgtctga acaagttagt ttaaatgaga taaataggta tatagaaata gaaaaggcag
     3001 tagttagaga gtttgatagt tacgaagatg aaaatct
//
LOCUS       DATGSI010000518         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_413864, whole genome shotgun sequence.
ACCESSION   DATGSI010000518 DATGSI010000000
VERSION     DATGSI010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413864"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VK031_08015"
     CDS             complement(<1..798)
                     /locus_tag="VK031_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HLS53905.1"
                     /translation="MLDKKIIEDVLYAALSKGGDFAEIFVEDKRNGHMKMVGGLLETN
                     VSGRDHGVGIRIFDKHHSIYAYTNDSSRENLIKVAKEAAAALNTEKIHLDLTLDKRKT
                     SNHNPIEISPIPVDKKEKVDLLKKGHEAAKNYDEVISQVTVNYLEEEQNVLIANTDGL
                     LVTDSRVRTRYAIQSVASKDGEMQVGHYAPGASMGFEFYKKIDVEEVAKEASRIAKTS
                     VYADYCPSGVMPVIIDNEFGGVLFHEACGHALEAEFVAKGTSAFANKL"
     gene            complement(891..1436)
                     /locus_tag="VK031_08020"
     CDS             complement(891..1436)
                     /locus_tag="VK031_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HLS53906.1"
                     /translation="MEKELEVKVLGLDLEKIEKRIIKLGGKLIAEEKQVNTLIDSKDR
                     PIKSFTDAYLRIRETKDLLNKEERVTLTLKKNISLAGVRENHEFNTEVEDKEVMLAIL
                     KELGFDKIEVGYKDRRSYQLMEARIDLDKWDEKTYPYPYLEIEVEDLKQLNEICSILK
                     IPRENISTKSIVELRKELNLI"
     gene            complement(1421..>2018)
                     /locus_tag="VK031_08025"
     CDS             complement(1421..>2018)
                     /locus_tag="VK031_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLS53907.1"
                     /translation="KNIPVSMAHSYASYEETMKGIEAGITIATHLYNGMREFNHREPG
                     IIGASLTDDRVYCEVIYDRFHLHDAAVKMALRMKGYDRIILVSDAMRAAGLEDGEYDL
                     GGQRVYVQDGKPKLESGSIAGSTLDLKEAVYNMVTYMQVPINEAVKMASLNPAKAIKV
                     DEEIGSIEIGKKADLILFDRDININKVFLGGQLAWKKN"
BASE COUNT          535 a          469 c          276 g          738 t
ORIGIN      
        1 cagcttattg gcaaaagcag aagtaccctt agccacaaat tctgcttcta aagcatgccc
       61 acaggcctca tgaaataata caccaccaaa ttcattatct ataattactg gcataacacc
      121 actagggcaa taatcggcat atacagaagt ttttgcgatc ctggaggcct cttttgctac
      181 ttcttccaca tctattttct tgtagaactc aaatcccatg gaggcaccag gagcataatg
      241 acctacctgc atctccccat cttttgaagc cactgactgg atggcatacc tggttcttac
      301 tcttgaatct gttactaaaa gcccatctgt attggcaatt aaaacatttt gctcttcctc
      361 caaataattt actgtcactt gagatattac ttcatcatag ttttttgcag cctcatggcc
      421 ctttttaagt aggtctactt tttccttttt atccactgga atcggggaaa tttctattgg
      481 gttgtggttg ctggttttcc ttttatccag ggttaaatct agatggattt tctcagtatt
      541 taaagctgct gcagcctctt tggccacctt tattaagttt tccctgctag aatcattggt
      601 ataggcatat atactatggt gtttatcaaa aattctaatt cctacaccat gatcccttcc
      661 agatacattg gtttccaata ggcctcctac cattttcata tggccatttc ttttgtcttc
      721 cacaaatatt tctgcaaaat ctccgccctt agataaggca gcatacaata catcttctat
      781 aatcttttta tctagcaatt tacgaccccc tattaaatat aaatgcttat tagcctattt
      841 aagattgaaa tggagaaacc ctcagaagaa ttaggattcc tccatttagt ttaaattaga
      901 tttaattctt tccttaattc tactatagac ttagtagata tattttctct tggtatcttt
      961 aaaatactac aaatctcatt taattgtttc aaatcttcta cttcaatttc tagataggga
     1021 taaggatatg ttttttcatc ccacttatcc aaatctatcc tggcctccat taactggtag
     1081 gaccttctat ccttgtaacc tacctctatt ttgtcaaatc ctaactcctt taaaattgcc
     1141 agcataactt ccttatcttc cacttctgta ttaaattcat gattttccct aacaccagcc
     1201 aggcttatat tcttttttaa ggttaaagtg accctttctt ccttatttaa taagtccttg
     1261 gtttctctaa ttcttaaata ggcatctgta aaagatttaa tcggcctatc ttttgaatct
     1321 attaaggtat ttacctgttt ttcttcagct attagttttc caccaagttt tattattctt
     1381 ttttctattt tttctaaatc tagacctaaa acttttacct ctaattcttt ttccatgcca
     1441 attgtcctcc aaggaatacc ttatttatat ttatatccct atcaaataga attagatcgg
     1501 cttttttccc tatttcaata ctgcctattt cttcatccac cttaattgcc tttgccggat
     1561 ttaggctggc catctttact gcttcattta taggcacctg catataggtt accatattgt
     1621 aaacagcttc cttaagatcc aaggtagagc ctgcaatgct acctgattct agtttgggtt
     1681 tcccatcttg aacataaacc ctttggcctc ccaaatcata ctctccatct tctagacctg
     1741 ctgccctcat ggcatctgat actaggatta tcctgtcata ccctttcatt ctcagggcca
     1801 tctttacagc tgcatcatga aggtggaatc tatcataaat tacctcacaa taaacccggt
     1861 catcagtcag ggaagcccct ataattccag gctccctatg gttaaactct ctcataccgt
     1921 tatataggtg ggtggcaatg gttatacctg cttcaatccc cttcattgtc tcttcatagc
     1981 tggcataaga atgggccata gacacaggaa tattcttt
//
LOCUS       DATGSI010000519         1201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_604392, whole genome shotgun sequence.
ACCESSION   DATGSI010000519 DATGSI010000000
VERSION     DATGSI010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604392"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VK031_08030"
     CDS             <1..1195
                     /locus_tag="VK031_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLS53908.1"
                     /translation="APPILTNIQREGKKLDVVAQVTKSGHVFVFNRETGKSIFPIEEV
                     EIPASVIPEEEVWPTQPIPSLPAPFSRQNLTEDDLNMYSPDYDSLLKVFRNSKKGRFV
                     PFSETPTIIFPGLNGGAEWGGAAVDPGGIMYVNSNEIPWVEWLIPTSLKKDSFSRGQN
                     IYRNSCGSCHRMDRTGNPGSGYPSLVNVKQRLTQDEVSKIISNGQGTMPGFTQLTSIE
                     KKELINFLFDKKEKEITTDLNTKIDSSNESEKVPWKIDGHRQFRDSEGVPGISPPWGT
                     LTAINLNTGKHQWRIPLGNLEKYNGKESLPTGTENYGGPVATAGGLLFIAATKDAKIR
                     AFNKKTGKLLWDFELPTGGTATPSTYEVNGKQYVVIAAGGGRLGLDKGDSIIAFALPE
                     SCCKN"
BASE COUNT          401 a          191 c          276 g          333 t
ORIGIN      
        1 ggctccacct attttaacta atatacagag agagggtaaa aagttagatg tagttgcaca
       61 agtaactaaa tctggccatg tgtttgtctt taatcgagaa acaggaaaat ctattttccc
      121 catagaagag gtagagattc ccgcatctgt cattccagaa gaagaggtct ggccaactca
      181 acccatacct tccttaccgg ctccatttag taggcaaaat cttacagagg atgatttgaa
      241 tatgtattca ccggattatg attctttgtt aaaggtattt cgtaattcta aaaagggaag
      301 atttgtaccg tttagtgaaa cgccaacgat tatatttcct ggtttgaacg gaggagcaga
      361 atggggcgga gctgcagtgg acccgggcgg tattatgtat gttaattcta atgagatacc
      421 atgggtagag tggttaatac cgacctctct gaagaaagat agttttagcc ggggccaaaa
      481 tatatatagg aatagttgtg gatcttgtca tagaatggac cgcacaggaa atcctggtag
      541 tggatatccg tcattggtta atgttaaaca aagattaacc caagatgaag tatcaaaaat
      601 tatatctaat gggcagggta cgatgcctgg ttttactcaa cttacttcga tagaaaaaaa
      661 agaactgatt aattttttat ttgacaagaa agaaaaagag attacaacag atttaaatac
      721 taaaattgat agtagcaatg aatctgaaaa ggttccttgg aagatagatg gacatagaca
      781 gtttcgcgat agcgagggcg taccgggcat ttccccacct tggggaactc taactgctat
      841 taatctaaat actggaaaac accagtggcg tattcccttg ggtaatttag aaaaatataa
      901 tggtaaagaa tctttaccta caggtacaga aaattatggg gggcctgtag ctacagctgg
      961 tggactgtta tttatagcag ctacaaagga cgctaagata agagcattta ataagaagac
     1021 tggtaaactg ttatgggact ttgaattacc aacaggagga actgcaaccc ccagtactta
     1081 tgaagttaat ggaaaacaat acgtagttat agcggctgga ggtggaagat tgggtttaga
     1141 taaaggagat tctattattg cttttgcact accagaatca tgttgtaaaa actgaagggg
     1201 g
//
LOCUS       DATGSI010000520         1440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_178731, whole genome shotgun sequence.
ACCESSION   DATGSI010000520 DATGSI010000000
VERSION     DATGSI010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178731"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..679)
                     /locus_tag="VK031_08035"
                     /pseudo
     CDS             complement(1..679)
                     /locus_tag="VK031_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(894..1409)
                     /gene="tpx"
                     /locus_tag="VK031_08040"
     CDS             complement(894..1409)
                     /gene="tpx"
                     /locus_tag="VK031_08040"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HLS53909.1"
                     /translation="MVEKRRGIVTMGGQPVTLVGREVKVGDKAPDFTALKRDLTEYSL
                     KDAKDKIKIISVVPSLDTGVCELQTIRFNEEAAKLEDVVILTISVDLPFAQDRFCSAN
                     SIDKVITLSDHKDLSFGLNFGFIMEEHRLLCRGIVVLDRDNTVRHVEYVKEVRNHPGY
                     DKALEEVKKLI"
BASE COUNT          395 a          298 c          192 g          555 t
ORIGIN      
        1 atatgcgtag catttttcct tctcactttt atatagcatt ccactatctg ggtcggtttt
       61 acttatcttt acttccttgt attctgtttt ttttactcat atcaaggggc ttcttgccat
      121 gtacttctct atcttcattt atttctttgt ctagtaattc ttggtagcta cgcattttct
      181 tttctaattt aactttattg tgcttcttct tattggcatt agcttttacg tgggtagaat
      241 ctatgaagac tgcatctgta tctatgaatc ctgcatctat tgcttgttct aggatacgca
      301 taaatatttt ttcaaatacg tctgtatctt taaatcttct aacatagttc tttccaaatg
      361 tagtaaagtg gggtatctta tcatgtagac catatcctag aaaccagcga taggctacat
      421 tagtttctat ttcagctata gtttgcctca ttgattttat cccaaagata tattggatga
      481 agataatttt aaataaaact acagggtcta tacttggtct gccggtttca gtactataca
      541 tgtcctttac taaatcatag ataaagctaa aatctatgca agcatcaagt tttctaacta
      601 aatgatcttt tgggaccaat tggtcaatag atatgatttc aacttgattt cttttatcag
      661 atttatttat cgttagcata ataacacctc atttaaaaat attttatata ttatatattc
      721 tacaaaaaag cagaaaatcc tttttgattt caaggatttt ctgctttttt ataaaaaatt
      781 tatactttgt cttcagtctg aaggaaggac gaaagtcctt ccttattcta tcttaagcat
      841 taccgcctaa tggtcgcttc tcattgtctc cgcatattct tcatatattg ttattatata
      901 agctttttca cttcctctag tgccttatca tagccggggt ggtttctcac ttcttttaca
      961 tattcaacat gtcttacagt attatcccta tccaaaacta ctattcccct acatagaagc
     1021 ctgtgttcct ccataatgaa tccaaaattt agaccaaaag acagatcctt atgatccgat
     1081 aatgtaataa ccttatctat actattagca ctgcagaatc tatcctgagc aaagggaaga
     1141 tccacactaa tggttaatat tactacatcc tctaatttgg cggcttcctc attgaaacgg
     1201 atagtttgga gttcacaaac ccccgtatcc aaggatggga ctacactgat tatcttaatc
     1261 ttgtcttttg catccttcaa tgaatactcc gttaaatccc ttttaagagc tgtaaaatct
     1321 ggtgccttat cccctacctt aacttccctg ccaactaatg ttacaggctg acctcccatg
     1381 gttactatgc cccttctctt ttctaccatc ttcatctccc ttgtcaattt aaatacttta
//
LOCUS       DATGSI010000521         7014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_651179, whole genome shotgun sequence.
ACCESSION   DATGSI010000521 DATGSI010000000
VERSION     DATGSI010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651179"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..180
                     /locus_tag="VK031_08045"
     CDS             28..180
                     /locus_tag="VK031_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53910.1"
                     /translation="MRKEIYNMTQKEINRLRVINQTIDKVETISGAAEALDLLKWKQA
                     LLIYLQ"
     gene            complement(113..502)
                     /locus_tag="VK031_08050"
                     /pseudo
     CDS             complement(113..502)
                     /locus_tag="VK031_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
     gene            complement(495..1082)
                     /locus_tag="VK031_08055"
     CDS             complement(495..1082)
                     /locus_tag="VK031_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HLS53911.1"
                     /translation="MSFKKLKVFLVLFKITFYISAFTFGGGYIVIPMMRRYFVHDLEL
                     ITEKKLMDMSAISHSSPGAIAVNVAVLIGYKIVGLAGAIVATIGTILPPLLILSVISI
                     FYKAFRDNAIVSALLKGMEARVAAIIVDILIDMWQGILKEENLLLTLMAPAAFIANYI
                     FNVNVLYIISFSATLCSLQVYIKSQTNRKEGIDNE"
     gene            1362..1838
                     /locus_tag="VK031_08060"
     CDS             1362..1838
                     /locus_tag="VK031_08060"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HLS53912.1"
                     /translation="MINDLDLKYLRRCIELAEIALEKGDQPFGSVLVSEDGKILAEGH
                     NEIASGDNTQHPEFNLARWAAENMNPEERSKATVYTSGEHCPMCAAAHGWVGLGRIVY
                     ATSSEQLVQWLEEMDIPQGRVKTLPILEVIRDTEVVGPVPELAEEVYRLHRQYYGK"
     gene            2505..2981
                     /locus_tag="VK031_08065"
     CDS             2505..2981
                     /locus_tag="VK031_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLS53913.1"
                     /translation="MKNKEFENLFMKYKGDIYRLAYTYVNNEVDALDIVQETAYQAYI
                     SRDTIRDKEKFKSWILKIAVNKSKDMLRKNRGILLEELSKARKLGSKDNDSINLFMDN
                     LKNLSLEEKNIIVQKIYFQYTFEIISEKLTIPIGTVKSRYYRALEKLRLEESKYER"
     gene            2971..4233
                     /locus_tag="VK031_08070"
     CDS             2971..4233
                     /locus_tag="VK031_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53914.1"
                     /translation="MKDRLEVNLNKIPLPENLDERIEKGFNRAKKEESIGRRWPKALA
                     ITAAVVTILATSIGIIGLDKVEAAIKQALQYVPGYNIVVDREVGEILALKDPVIYRKN
                     NVFINIKAASKLGSHLNISVESNLKLGDETKDYVDIHLKDREGKTIKYDRWSTAAGGE
                     FWQGDYYFKVDEDEEAFILEMEGHEIFFTLEKTKEVESLRQLGGYHSKKGIHIVAIKK
                     MVEDRLMVSLLNQSDEKTIYEYPFEENLIGEGWGKRGDLEKSMYIIDDKGQKLYPQIP
                     SSFGNSLSDFYFDTVDKEGLELVLPYVKVIYDDLESEKVKIKKPKDGEIIDINKRVSL
                     GEFEIEILDIKNKGEELVVSLDSRSPKDEILEEVRIRGIDGYGIWFNEKTGYVELFIN
                     KEDVGRSFSLYFESPVSLLLGEWRINLD"
     gene            4443..5276
                     /locus_tag="VK031_08075"
     CDS             4443..5276
                     /locus_tag="VK031_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HLS53915.1"
                     /translation="MKFISWNIDSLNAALTGVSARAELSREVLDTIIGHDPEVIALQE
                     TKLPSTGPTKKHLSILDKMFPDYEIVWNSSREPARKGYAGTMFLYKKNLDPLVTFPVL
                     DAPDTMDYEGRIITLEFDDFYLTQVYTPNAGEGLRRLTERQVWDEKYADYLEKLDKEK
                     YVIATGDFNVAHKEIDLAHPENNRRSPGFTDEEREGFTNLLAKGFTDTFRYIHGDVEG
                     VYSWWAQRARTSKINNSGWRIDYWLVSNRIAGRVRKSEMIDSGPRQDHTPLLLEIDLN
                     F"
     gene            5657..6532
                     /locus_tag="VK031_08080"
     CDS             5657..6532
                     /locus_tag="VK031_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HLS53916.1"
                     /translation="MTKIIMDSTADLPKDLINKYQIDILPLRLSIGDKEYIDKVTISL
                     EEVYDAMRNNIYPKTSQPNPKTSYDLFETYASQGKDFIFFSFSSKLSSTYQTSYLIIK
                     ELKSRFPQVKMDIIDTKGGSFASGLIALQAAIMSKEKIEFEDIVRISRSNVLHVEHIF
                     TIDDLDWLVKGGRIKKSEAIVGKTLNIKPILEVQDGKMIVIDKIRGRKRALKTVVDLV
                     EERIEKFPNQIVGITHADDLGAANRVKNLVQEKIGPNNILVEKIGSVLGSHLGIGGVG
                     VFFLNNKPEFYVSGF"
BASE COUNT         2592 a          896 c         1423 g         2103 t
ORIGIN      
        1 ttaaaattat tctgataaaa tgtcagtatg agaaaggaaa tatataatat gactcaaaaa
       61 gaaattaaca gacttagagt tatcaatcaa acaattgata aggtagaaac tattagtgga
      121 gctgctgaag ctttagatct attgaaatgg aagcaagccc ttttaatata tttgcaatag
      181 aatctacatt cttattcttc ataaaaaacc tataaagtaa gatagaaata agaaaaccag
      241 aaaaaatact acctacagta gcaatgatag ctccccaggt acctgctatc cttatgtcta
      301 caaaagatgc agtgttgaca accaaagggc caggagtcat ttgagatatg gtgataatat
      361 ctgtatactc atttagagta agccaatttg ttcagttaac aacttgaacc tgtatcaggg
      421 ggataatggc atatcctcca ccaatactaa ggctacctat acgaaaaaat gtatggaaca
      481 aggtaaatat aattttattc attgtctata ccttctttcc tattagtctg acttttaata
      541 tatacttgca ggctgcataa agtagcagaa aaggaaatta tatacaatac attaacatta
      601 aaaatgtaat tggctataaa ggcagctgga gccatcaggg tcaacaatag gttctcttcc
      661 tttaaaattc cttgccacat atctattaaa atatcaacaa ttatagctgc aactcttgcc
      721 tccattcctt tcaatagggc agaaacaata gcattatctc tgaaagcctt ataaaaaata
      781 gatataacag ataatattaa taaaggaggc aggatagtgc caatggttgc aacgatagcc
      841 ccggcaagtc ccactatctt gtagccaatt agtacagcaa cattaacagc aatagctcct
      901 gggctggaat gggaaatggc agacatatcc attaacttct tttctgtaat taattctaaa
      961 tcatgaacaa aatatctcct catcattgga ataactatat atccaccacc aaaggtaaag
     1021 gcactaatat aaaatgtaat tttgaaaagg actagaaata cttttaattt tttaaagctc
     1081 atatagaaac cctccaaata ataaacctaa ttacctatta tacatctaaa gttatataag
     1141 taaaatgaaa ggcaggcaag gaattttgaa actgagcata tgaatttttc aaaggaagaa
     1201 tatctagact attttgatta tatgcagttg ctgataatgg ggctaaaaat agctaataat
     1261 aagtagcctg gcttaattat aagactatga tgttaggatt taaggatatc tgggtataat
     1321 ttttactaaa ttatggcttt gataaagtag gaggttttgg gatgataaat gatctagatt
     1381 taaaatattt acgaagatgt attgagttag cagaaattgc tttagaaaag ggggatcaac
     1441 cctttggttc agtcttggtt tcggaagatg gtaagatttt agcagaggga cataatgaaa
     1501 tagcaagtgg agacaatacc cagcatccag aatttaatct ggcccgttgg gcagccgaaa
     1561 atatgaatcc tgaggaaaga agtaaggcca cagtatatac ttcaggagaa cactgcccta
     1621 tgtgcgcagc agcccatggt tgggtaggcc tgggaagaat tgtttatgca acttcttcag
     1681 aacaattggt tcaatggctg gaagaaatgg acataccaca gggaagggta aaaactttgc
     1741 ctatattaga agttattaga gacacagagg tggttgggcc tgttccagaa ttggcagagg
     1801 aggtatatag gcttcatcgc caatattatg gaaaataata taagcattta ataaactata
     1861 aattatttta aatacttttc ctgcatatat aaaaggttaa ttggttttat actgattaac
     1921 cttttaataa tttaagagaa gggaaagcag acagagattt taccttttct atctaatgtg
     1981 gccagaattt ttaatcttag agcccttttc tttataatac aattagtata cataggggcg
     2041 aggacataag gccaagacct aaggaaggtt agttgccagc tacctctatt ttcataatat
     2101 gttgggagga tttgttggat ttttattaat ggccattatt tcctatcata gaaaatagaa
     2161 acatcatttt ctcgttgaat tttaatgggt aggtggtttc atttaactga attatttctt
     2221 aaatggaatt gataaaaagg tagaaagaca agatggtgaa attagggtaa ttaaggacgt
     2281 aaactaaata atattaaaat acataagagg atgggaagct tatgctaacc atcctctctt
     2341 cttcttataa agatgaaaac agatccatca aagggcaagt ggtataatga aaattagatg
     2401 ggaaatataa aaaattatat aaaaaaattt tttgaagcta ttttggactt taatccgtct
     2461 tatatataga agatcttcta gagaagaggt gtattgatat taaaataaaa aataaagaat
     2521 ttgaaaattt atttatgaag tataaaggag atatctacag gctagcctac acttatgtaa
     2581 ataatgaggt agatgcctta gacattgtgc aggaaactgc ctaccaggcc tatatatcaa
     2641 gggatacaat aagagacaaa gagaaattta aatcctggat attaaagatt gcagtaaata
     2701 agtcaaagga tatgcttaga aaaaacaggg gaattctatt ggaggagtta tctaaggcaa
     2761 gaaaattagg gagtaaggat aatgatagta tcaatttatt tatggacaat ttgaaaaatt
     2821 tgtccttaga agaaaaaaat attattgtcc aaaaaatata ttttcaatat acctttgaaa
     2881 taatttccga aaagttaact atccctattg gtacagttaa aagtaggtat tacagggctt
     2941 tggaaaagtt aagattggag gagtccaaat atgaaagata ggttagaagt taatttaaat
     3001 aaaattccat taccagaaaa cttagatgaa aggattgaaa aggggtttaa tagggctaag
     3061 aaggaagaaa gtattggcag gagatggcca aaagctttgg ctataactgc agctgttgtg
     3121 accatacttg ctacttcaat agggataatt ggactagaca aggtagaagc agccattaaa
     3181 caggctctac aatatgtgcc aggctataat attgtggtag atagggaagt aggagaaata
     3241 ctggctctta aggatcctgt tatatacagg aagaataatg tctttataaa tataaaagct
     3301 gcatctaaac taggcagtca cctaaatata tctgtagaaa gcaatttgaa actaggggat
     3361 gaaactaaag attatgtaga cattcattta aaagataggg aaggaaaaac tattaaatat
     3421 gatagatgga gtacggcagc tggcggagaa ttttggcagg gtgattatta ttttaaggta
     3481 gatgaagatg aagaagcttt tatccttgaa atggaaggac atgaaatatt ctttaccttg
     3541 gagaaaacca aggaggtgga aagtttaaga cagctaggtg gttaccatag taaaaagggg
     3601 attcatatag tggctataaa aaagatggtg gaagataggc taatggtaag cttattaaac
     3661 cagtcagatg aaaaaacaat ttatgagtat ccctttgagg agaacctaat aggtgaaggc
     3721 tggggcaaga gaggagattt ggaaaaaagt atgtatataa tcgatgataa ggggcaaaag
     3781 ttatatccac aaataccatc ttcctttgga aattccttat cagactttta ttttgacaca
     3841 gtagataagg aaggcttaga actggtcctg ccatatgtaa aggtaatata tgatgattta
     3901 gaatcggaga aggttaagat caaaaaacct aaggacggag aaataattga tataaataag
     3961 agagtctctc tgggagaatt tgaaatagaa attttagata taaaaaataa gggtgaggag
     4021 ctggttgtta gcttagatag taggtcccca aaagatgaaa ttttggaaga agttagaatt
     4081 aggggaatcg atggctatgg aatatggttc aatgaaaaaa caggctatgt ggaactcttt
     4141 ataaacaagg aggatgtagg aaggagcttt tcactttact ttgaatcacc agtatccttg
     4201 cttttaggag agtggagaat aaatttagat taggaaggtg tttaaagtac taatatactt
     4261 gcttcggcat aggtataata aaacaaaatc tagggctaac taggttctct ttactctttc
     4321 cacttgaaga ataactcagc ctagttatat aatagttgta atgatattga atctaaaata
     4381 ttgggtataa aaaatatagt tatataggag aaaagtagat taagaaacta agggggaaaa
     4441 taatgaagtt tatttcttgg aatattgatt cattaaatgc agctttaaca ggagtttcag
     4501 ccagggctga gttgtctagg gaagtattgg atacaattat agggcatgat ccagaagtta
     4561 tagccttaca ggagacaaaa ttacctagta ctgggccaac taaaaaacat ttatctattt
     4621 tagataagat gtttccggac tatgaaatag tatggaatag ctctagggag cctgctagaa
     4681 aaggttatgc aggtaccatg tttctatata agaagaattt agatccactt gttaccttcc
     4741 ctgttttaga tgcaccagat actatggatt atgaaggaag gattatcact cttgaatttg
     4801 atgattttta tttaacccag gtctatacgc ctaatgcagg agaaggttta aggcgactta
     4861 cagaaagaca ggtttgggat gaaaaatatg ctgattattt ggaaaaactt gataaggaaa
     4921 aatatgttat tgccacagga gattttaatg tggcccataa ggaaatagac ctggctcatc
     4981 ctgaaaacaa tcgccgctca ccaggcttta cagatgagga aagagagggc tttactaatc
     5041 ttttagcaaa gggatttact gacaccttcc gttatattca tggagatgtg gaaggggtat
     5101 atagctggtg ggcacaaagg gcgaggacca gtaagataaa caactctggc tggaggattg
     5161 actattggct agtcagcaat aggatagcag gcagggttag gaaatcagag atgattgatt
     5221 ctggacctag gcaagatcat acgcctttat tactggaaat tgatttaaac ttttaagaag
     5281 gctacgatga aattgaaata atttaagagt tttaaattcc agaaaggctt agctctttaa
     5341 agagttaagc cttgtttttt attctaagaa tcaaaggaag cctattttac ctgtagcctg
     5401 tataaagaaa gtttggagtc agagtgccct actttataat aaatgtcctg gcatatccaa
     5461 tatattatga gtatttaata attatggtgc cgactattac tagcttccta taaaggtcat
     5521 ttttaaattt ttagtaaagt ggggagcctt aaatgtgggc caagggtaag cactaattct
     5581 agtgggaaag tagtataatt ttaaatttat gatataattg tctgttaaga tttaaacaat
     5641 aaaggaggat attttcatga caaagataat aatggatagt actgctgatc tccctaagga
     5701 tcttattaat aagtatcaga ttgatattct gcctcttaga ctaagtattg gtgacaagga
     5761 gtacatagat aaggtaacca tatccttaga agaagtatat gatgctatga gaaacaatat
     5821 atatcctaaa acatctcaac ctaatcctaa aaccagctat gatttatttg aaacatatgc
     5881 atcacaagga aaagatttta tatttttttc tttttcatct aaactatcca gtacatatca
     5941 gacttcttat ttgattataa aagaattaaa aagcaggttt cctcaggtta aaatggatat
     6001 aatagatact aaaggcgggt cctttgcatc aggattaata gccttgcaag ctgcaattat
     6061 gtccaaagaa aagattgaat ttgaggatat agtaaggatt tcaagatcca atgtattaca
     6121 tgtggagcat atatttacca tagatgattt agattggctt gtaaaaggtg gcagaattaa
     6181 aaaaagtgag gcaattgtag gtaagacttt aaatataaag cctattttag aggtacaaga
     6241 tggaaaaatg atagtaattg ataaaataag aggtagaaaa agggccttaa aaactgttgt
     6301 agacttggtt gaggaaagga tagaaaaatt tcctaaccaa atcgtaggaa taacccatgc
     6361 tgatgactta ggtgcagcta acagggttaa gaacctggtt caggaaaaga taggaccaaa
     6421 taatattttg gttgaaaaaa ttggtagtgt tttaggttct catttaggta ttggaggtgt
     6481 gggagtcttt tttttaaata ataagccaga attttatgta agtggttttt agtttaattt
     6541 aagaaaattt atttcataac aaatatacag tattttaaat attggacatt tgaatattat
     6601 aaaactaatg ttaaaaaatt accaattaat ttaaaatgtc ttgttatata caccaaagta
     6661 tgttaaaatt taaacaaaca gttggaagtt taattaggtg ttagtggaat taagccttta
     6721 acttagtgag agtaaattga tatgattgat aaatttaaca tatggggcaa cttatgccta
     6781 aatatgttaa atttaaaaca atagattaaa gctgattact ataattaagg aggaataaaa
     6841 atgaaaatag taattttagg tggagctggt gggcaaggtt tatactcaac taaagaaatc
     6901 attagaggtg gagtttttaa ggagatagtg gtggcaagta gaaatcttga gaaaacccag
     6961 ggaattgttg atggactaaa tgcttcaaat gtaagagcag cacaggtaga tgtt
//
LOCUS       DATGSI010000522         1183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_507830, whole genome shotgun sequence.
ACCESSION   DATGSI010000522 DATGSI010000000
VERSION     DATGSI010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507830"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..891
                     /locus_tag="VK031_08085"
     CDS             94..891
                     /locus_tag="VK031_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53917.1"
                     /translation="MGTELLLEYHKKATESLSPLLYQFKPHIGSFGVNVPTYRHQFST
                     ETEYIPYYMIRSDTFNIMSENLEGSTLPNPVEHIVYNHSTINRLAFAGIGFDQISSII
                     PGCHEGYSALPYYLRYNGSVIMMLATPTYRVREAFSLVREAVINSSLYRELGEEAHTE
                     KGSQFLASVYMNDMIDKDSRVQELLYGEATAIINPDMIDPYYSQPITDLNRFPLINAS
                     KKIISNDTDYINSLLLVDKDVPKFSDIDGPEEYSLKAIRSFYKDLNK"
     gene            950..>1183
                     /locus_tag="VK031_08090"
     CDS             950..>1183
                     /locus_tag="VK031_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53918.1"
                     /translation="MIDVNDTAEIYDEETSPTIRNGQIKVRMKVETVVEISYNIDLIH
                     RIGAKETIVESVDEFLKKMKSNSYEVRQSVAERI"
BASE COUNT          418 a          185 c          221 g          359 t
ORIGIN      
        1 taccaactat attactaaca gagatgcaga tatgagggag cttccttttg agactaaata
       61 tatagcgtca atgttcctct ttgaaacaaa ctaatgggta ctgaattact tctagaatac
      121 cataagaaag caactgaaag tctatctcct ttactgtatc aatttaagcc acatattgga
      181 tcatttgggg ttaatgtacc cacctataga catcagttta gtacagaaac tgaatatata
      241 ccctattata tgatcagatc tgacacattc aatatcatgt cagagaactt agaaggctct
      301 acacttccta accctgttga acatatagtt tacaatcatt ctactattaa tagattggct
      361 ttcgctggga taggttttga tcaaatttca tcaattatcc ccggttgtca tgaaggatac
      421 tcagcactcc catactatct gagatataat ggctctgtga tcatgatgct tgctacacca
      481 acatatagag tgagggaagc atttagccta gttagagaag ctgtaataaa tagtagctta
      541 tatagagaat taggggaaga ggcacatact gaaaagggaa gtcaattcct tgcctctgta
      601 tatatgaatg atatgattga taaagatagc agagtacaag agctattata tggagaggcc
      661 acggctataa taaatccaga tatgattgat ccttattata gtcagcctat aaccgactta
      721 aacagatttc ctttaataaa cgcttcaaag aagataatca gtaacgacac tgattatata
      781 aatagtcttc tcttagtgga taaagatgtc ccaaagttta gtgatataga tggacctgaa
      841 gagtattcct taaaagctat tagaagcttt tacaaagatt taaataagta actgaaattg
      901 gatgcctcca tatattgggg gcatcttcat tttttaatcc ttttgtgata tgattgatgt
      961 taatgataca gctgagatat atgacgagga gacgtctcct acaatacgca atggtcaaat
     1021 taaggtaaga atgaaggtgg aaactgttgt agaaatatca tataacatag atttaataca
     1081 tcgtatagga gctaaagaaa ctatagttga gtctgttgat gagttcttaa agaagatgaa
     1141 aagcaatagt tatgaagtaa gacaatcagt agctgagaga ata
//
LOCUS       DATGSI010000523         5364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_226111, whole genome shotgun sequence.
ACCESSION   DATGSI010000523 DATGSI010000000
VERSION     DATGSI010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226111"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VK031_08095"
     CDS             complement(<1..401)
                     /locus_tag="VK031_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HLS53919.1"
                     /translation="MSFDGVVTNAVSNELSATLLGGRVDKIYQPEDDEIILNIRNKGK
                     NFRLLISASSHNPRIYLTEHTTKNPAMPPMFCMLLRKHLGSGTILDIKQGAFDRILYI
                     DISSIDELGSLTEKRLVVEIMGRHSNIILID"
     gene            585..1466
                     /locus_tag="VK031_08100"
     CDS             585..1466
                     /locus_tag="VK031_08100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HLS53920.1"
                     /translation="MVRSMTGYGRGEYENDLYRVKIEMKSLNHRYNDINIKLPRHILY
                     LEEHIKKVITKYIYRGKIDVFINLEYISDSPLEVKVDLPLAKSYKLALDSLCKELVID
                     EDVSLDHLINIPDIITTERKGVDEEAILDCLDHALEMALENIVKMRESEGNILKQDIL
                     SKLANLDRYLDRIEKRAPRLVLEYEERLRERIEELLDVSNELDEDRLNNEIAYFIDKK
                     GIDEEIVRLESHIKQLESILEEEGAIGRKLDFLIQEFNREINTIGSKSNDEEISKHVI
                     EFKAELERIREQVQNIE"
     gene            1480..1737
                     /locus_tag="VK031_08105"
     CDS             1480..1737
                     /locus_tag="VK031_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HLS53921.1"
                     /translation="MSIRLINIGFGNMVSANRIISVISPESAPIKRLIQEGRDNGLLI
                     DATYGRRTRAVIITDSDHIILAAIQPETIANRLTNKDDNNK"
     gene            1755..2378
                     /gene="gmk"
                     /locus_tag="VK031_08110"
     CDS             1755..2378
                     /gene="gmk"
                     /locus_tag="VK031_08110"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HLS53922.1"
                     /translation="MSKGFLLVISGPSGSGKGTVSQALLKRNRNIVFSISATTRKRRL
                     GEKDGVNYFFVDEEKFQQMVENNEFLEHALVHNNYYGTPREFVLEEIEKGEIVLLEID
                     VQGAMQVKKNYKNVVFVFLLPPTMDELKNRIIKRGTETKEDIERRFRNAFQELDFVGE
                     YDYFVVNDKVESAVEDIEAIIRAEKLRVKRHSDIKKKVISQKEEDKC"
     gene            2372..2581
                     /gene="rpoZ"
                     /locus_tag="VK031_08115"
     CDS             2372..2581
                     /gene="rpoZ"
                     /locus_tag="VK031_08115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HLS53923.1"
                     /translation="MLNPSFKGVLREGDSKYTLVMLIAKRARQLIDGAEPLIETESKK
                     PVTIAIEEIVEGKIKYENPPADGIK"
     gene            2590..3795
                     /gene="coaBC"
                     /locus_tag="VK031_08120"
     CDS             2590..3795
                     /gene="coaBC"
                     /locus_tag="VK031_08120"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HLS53924.1"
                     /translation="MLKGKHIVLGVTGGIAVYKAADLVSRLKKQGASVEVIMTEAAEE
                     FVDPLTFQTMSLNPVHTDMFREIEKYDVEHISLAQKSDIILIAPATANTIGKIANGIA
                     DNLLTTVVMASTAKVIFAPAMNTFMYKNPIVQENIKKLEGLDYEFIEPGVGLLACGDY
                     GAGKMAEPEVIVDYLINSINKKDLKGREILITAGPTIEPIDPVRYITNHSSGKMGYSL
                     AEEATKRGANVTLISGPTNLEAPKGVRLIKVKTTQEMFKAVEDNFEQCDALIKSAAPA
                     DYRPEHRQERKIKKKDDNDELNIKMVRNPDIAKYFGNRKGNRVVIGFAAETNNLIEHA
                     KVKLKSKNLDFIVANDVTQGGAGFGTDTNIISVVDANEDVTDYPKMGKKEVANIILDR
                     LVDVFKNES"
     gene            3838..>5364
                     /gene="priA"
                     /locus_tag="VK031_08125"
     CDS             3838..>5364
                     /gene="priA"
                     /locus_tag="VK031_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HLS53925.1"
                     /translation="MSIKYADLIIDNTSTELDQLFTYIVGEDLVDIIRPGMRVIVPFG
                     LGNKIIKGLVVNVKSHYQGQYKLKEIIDVLDDKPIISNDLINLALWMKKEYLATYLDA
                     FQPVLPPGNFKEVRTFVSLLREGKKVYKKEEKKIINYLKKRQVVDLDRLKRELKIRQI
                     NKYLNNLENKGIISTSIDIKTTVQKKKEKWVKLLDKCITLEELDEIIGKRAYKQREIA
                     KYLLTTKEVSVQDLLEKLNTSLNTLRALEEKGVIHIFEKEVHREPIRRKIPVYKKHKL
                     SKKQQYILDEIVTGIHKENRRNFLIHGVTGSGKTEVYLQLVEKMLEENKDSIILVPEI
                     ALTPQTIDRFVGRFGKEVAILHSRLSYGERFDQWRKIKEGQVKIVVGARSAIFAPFQN
                     LGLIIIDEEHESTYKSSQNPKYDTIEVAYKRAEIESAALVLGTATPSLETYYKAQLGK
                     FALLELPGRINKTQLPQVQLVDMRDELAGGNKSIFSLAMYKEIKENLDRKKQTILFLN
                     R"
BASE COUNT         2032 a          711 c         1131 g         1490 t
ORIGIN      
        1 ttgtctatca agataatatt gctatgtcgg cccataattt caactaccaa tcttttttca
       61 gttaaggaac ctaactcatc aatagaagat atgtctatat aaagaatcct atcaaaggcc
      121 ccttgtttta tatccagtat ggtgccactg cctaagtgct ttcttaaaag catacagaac
      181 atgggaggca tagcagggtt ctttgtagta tgttctgtta agtaaattct aggattgtgg
      241 ctactggcag atattaatag gcgaaaattc tttcccttgt ttcgaatatt taatataatc
      301 tcatcatctt caggttggta aattttatct accctgccac ctaataaagt ggcggataat
      361 tcatttgaaa cagcattagt tacaactcca tcaaaggaca taatcattcc tccaaatatt
      421 gtttaataat caaagtatat cattttatcc taattttata aaccttagta aaagataaag
      481 gaattattaa ggtttcttaa caaatagtag actattatta taagaggttg tataatacat
      541 atttaaggaa gcaatatatg gatttactat aatgaggtga tgttttggta cgaagtatga
      601 caggatatgg caggggagaa tatgagaatg atttatatag ggtaaaaatt gaaatgaaat
      661 cccttaatca taggtataat gatataaata taaaattgcc taggcatata ttatacctgg
      721 aggaacatat aaaaaaggtc attactaaat atatttaccg gggaaagata gatgttttca
      781 ttaatttaga atatattagt gattcccctt tggaggtaaa ggtggatctg cctttagcca
      841 aatcctataa attggcccta gatagcttat gtaaggaatt ggttatagat gaggatgtca
      901 gcctggatca tttaattaat atacccgaca ttataacaac ggaaagaaaa ggtgtagatg
      961 aagaagcaat actcgattgc ctggatcatg ctttggagat ggccttagaa aatattgtaa
     1021 aaatgagaga aagtgaagga aatatcttaa agcaagatat cttatctaaa ttagctaatc
     1081 tagataggta tttagataga atagaaaaaa gggcacctag gttagtatta gaatatgaag
     1141 aaaggcttag ggaaaggatt gaagaattac tggatgtaag caatgaatta gatgaggata
     1201 ggcttaataa tgaaattgct tattttattg ataagaaggg aatagatgag gaaatagtaa
     1261 gactagagag ccatataaaa cagcttgaat ccattctgga ggaagaagga gccattggca
     1321 ggaagttaga ttttctaata caagagttta acagagagat caatactatt ggcagcaagt
     1381 ccaatgatga ggaaatttct aagcatgtta ttgagtttaa ggctgaactg gaaagaataa
     1441 gagagcaagt acaaaacata gagtaatgga ggttttgata tgtccataag attaataaat
     1501 attgggtttg gaaatatggt atccgccaat agaataattt cggttattag ccctgaatca
     1561 gcccctataa agagattgat tcaagagggt agggacaatg ggctactgat agatgccaca
     1621 tatggcaggc ggacaagggc agttataata actgatagcg accatattat cttagcagct
     1681 attcaacctg aaacaattgc aaatagatta acaaacaagg atgataacaa taaataaaag
     1741 gttggtgaca aaaattgtct aaaggatttt tactagttat atcaggccca tctggcagtg
     1801 gcaagggtac agtcagtcag gccctgttaa aaagaaatag aaatattgta ttttcaattt
     1861 ctgccactac cagaaaaaga aggcttggag aaaaggatgg agtaaattat ttctttgtgg
     1921 atgaagaaaa gtttcaacag atggtggaga ataatgaatt tttagaacat gcacttgtcc
     1981 ataataatta ttatggtacc cccagagaat ttgttctaga ggaaatagaa aagggagaaa
     2041 tagtcctatt ggaaatagat gtacagggag ccatgcaggt taagaaaaac tataagaatg
     2101 tagtatttgt atttttgcta ccgcctacca tggatgaatt aaaaaatagg attataaaaa
     2161 ggggaactga aacaaaagag gatatagaaa gacgttttag gaatgctttc caggaattag
     2221 attttgtagg tgaatatgat tacttcgtgg taaatgacaa ggtggagagt gcagtagaag
     2281 atatagaggc cattatcaga gctgaaaaac taagagttaa aagacattca gatattaaga
     2341 aaaaggttat tagtcaaaag gaggaagata aatgttaaat ccatctttta agggtgtttt
     2401 aagggaaggg gatagcaaat atacactggt tatgttaatt gctaaaaggg ccagacaact
     2461 tattgatgga gccgagccct taatagagac agaatcaaag aagcctgtaa ccattgcaat
     2521 agaagaaata gtggaaggta aaattaaata tgaaaatcct ccagcagatg ggattaagta
     2581 ggtgacaaga tgctaaaggg aaaacatatt gtattaggtg taactggtgg aattgcagta
     2641 tacaaggcag cagacctggt tagcaggctg aaaaaacaag gggccagtgt agaagttata
     2701 atgacagaag cagctgagga atttgtagat cctttaacct ttcaaacaat gtctttaaat
     2761 cctgttcata ctgatatgtt tagggaaata gaaaaatatg atgttgaaca tatatctttg
     2821 gcacaaaaat cagatattat attaattgct ccagcaacag ccaatactat tgggaaaata
     2881 gccaatggta ttgcagacaa tttattgacg acagttgtca tggcatccac tgccaaagtt
     2941 atatttgccc ctgccatgaa cactttcatg tataaaaacc caatagttca agaaaatatt
     3001 aaaaagctgg aaggcttgga ctatgaattt atagagccag gggtaggcct actggcttgt
     3061 ggagattatg gggccggtaa aatggcggaa cctgaagtta ttgttgatta tttaattaac
     3121 agcatcaaca agaaagattt aaagggtagg gaaatactga tcactgctgg tcctaccata
     3181 gagccaatag atccagtaag atatattacc aatcattcca gtggaaagat gggttatagt
     3241 ttggcagagg aggccaccaa acgaggtgct aatgtcactt taatatctgg gccaacaaat
     3301 ttggaagcgc caaaaggagt tagattaata aaagttaaaa ctactcaaga gatgtttaag
     3361 gcagtagagg ataattttga gcaatgtgat gcattaataa agtcagctgc accagcagac
     3421 tataggccag agcacaggca agaaagaaaa attaagaaaa aagatgataa cgatgaatta
     3481 aatattaaaa tggttagaaa tcctgatatt gcaaaatact ttggcaatag gaaaggtaac
     3541 agggtagtta taggttttgc tgcagaaaca aataatttga tagaacatgc aaaagttaag
     3601 ttaaagagta agaatcttga ttttattgta gccaatgatg taactcaagg tggtgcaggt
     3661 tttggcacag atactaatat tattagtgtt gtagatgcta atgaagatgt gactgattac
     3721 cctaaaatgg gtaaaaaaga agtagccaat ataattttag ataggttggt agatgttttt
     3781 aaaaatgaaa gctagttaat ctagctttca ttttttcaaa ataaagggga taatcatatg
     3841 tctataaaat atgcagattt aataattgat aatacatcaa cagaattaga tcaacttttt
     3901 acctatattg taggtgaaga tctagttgat ataatcaggc cgggaatgcg ggttatagtg
     3961 ccctttggat taggaaataa gataataaag ggcctggtag taaatgttaa atcccactac
     4021 caaggccaat acaagttaaa agaaatcata gatgtgttgg atgataagcc tattatttca
     4081 aatgatctaa taaacttagc cttatggatg aagaaggagt atttggcaac ttatctagat
     4141 gcctttcaac cagttttgcc accaggtaat tttaaagaag ttagaacctt tgtaagcctg
     4201 ttaagagaag gtaaaaaggt ttataaaaaa gaggagaaga aaataattaa ctacctaaag
     4261 aaaagacagg tagttgactt agataggctg aagagggagt taaaaataag gcagatcaat
     4321 aaatatctaa ataatttaga aaacaaggga ataatatcta caagtataga tataaaaacc
     4381 acagttcaaa agaaaaaaga gaagtgggtg aagctactcg acaagtgtat aactttagaa
     4441 gagttagatg agataatagg aaagagggcc tataaacaaa gagagatagc caaatatttg
     4501 ttgaccacaa aagaagtttc tgtgcaagat ttgcttgaaa aactaaatac cagtttaaat
     4561 actctaaggg ccttagagga aaaaggagtc atccatatct ttgaaaagga ggttcatagg
     4621 gaacccataa ggagaaaaat ccctgtttac aagaagcata aactaagtaa gaaacagcaa
     4681 tatatccttg atgaaatagt aactggtata cataaagaaa acaggagaaa tttcctaatt
     4741 catggagtta caggcagtgg aaaaactgag gtataccttc aattagtaga aaaaatgtta
     4801 gaagagaata aagactccat aatcctagtg cctgaaatag ccttaacccc ccaaactatt
     4861 gataggtttg tagggagatt tggaaaggaa gttgctattc tccatagtcg cttatcttat
     4921 ggggaaaggt ttgaccaatg gagaaaaata aaggaagggc aggtaaaaat tgtagtaggg
     4981 gccagatcag ctatttttgc cccctttcaa aacttaggat taatcattat tgatgaggaa
     5041 catgaatcaa cctataaatc ttcccaaaat cccaaatatg acactataga ggtggcttat
     5101 aaaagagcag aaatagaatc tgctgctcta gttttaggaa ctgcaactcc ttcgttggag
     5161 acctattata aggctcagtt gggtaaattt gcgctactag aattaccagg aagaataaac
     5221 aaaacacaac tacctcaggt tcaactagtt gatatgagag atgagttggc aggtggcaat
     5281 aagtcgatct ttagtctggc catgtataaa gaaattaaag aaaatttaga tcggaaaaag
     5341 caaaccattc tttttttaaa ccga
//
LOCUS       DATGSI010000524         1584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_556991, whole genome shotgun sequence.
ACCESSION   DATGSI010000524 DATGSI010000000
VERSION     DATGSI010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556991"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..306)
                     /locus_tag="VK031_08130"
     CDS             complement(100..306)
                     /locus_tag="VK031_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53926.1"
                     /translation="MPYKLKLEKLEESSKEKIESEIKYLSEENTELRRTIREGRDARK
                     VLEKNEVRMKELMECYLGLKEEEK"
     gene            complement(365..514)
                     /locus_tag="VK031_08135"
     CDS             complement(365..514)
                     /locus_tag="VK031_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53927.1"
                     /translation="MSITYENSAKEITLGQAIDAHKHGIAVIVNDGRDITVVIEKEPT
                     TDQVK"
     gene            complement(528..716)
                     /locus_tag="VK031_08140"
     CDS             complement(528..716)
                     /locus_tag="VK031_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53928.1"
                     /translation="MTREEREKRKKEQKEQFINDVNSCIRVLETLKKFDSMYIPNHIN
                     SAKVTRARLVIHDILKKY"
     gene            complement(713..871)
                     /locus_tag="VK031_08145"
     CDS             complement(713..871)
                     /locus_tag="VK031_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53929.1"
                     /translation="MDTSQDKLAMGDTVFWSSVYSFHRRIEEGAKDHTVMAVLFIKKR
                     NRKGEFIV"
     gene            complement(897..1082)
                     /locus_tag="VK031_08150"
     CDS             complement(897..1082)
                     /locus_tag="VK031_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53930.1"
                     /translation="MEKNISVKEAAEILGVSEQFIRIGLQRGKLPIGTAVKMSSRWTY
                     HISPKLLKEYIGEERKM"
     gene            complement(1060..1404)
                     /locus_tag="VK031_08155"
     CDS             complement(1060..1404)
                     /locus_tag="VK031_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HLS53931.1"
                     /translation="MNKERQAFRITDRGIIAKLKFENKWLRIGLVITSSIIAVLVSIL
                     IFIWISNIHGKGYTHSETVEIVRRDGTPDIKMNTKWTQKGRLFLYELLKENGILPIIE
                     QGDDMDGKKHIS"
BASE COUNT          386 a          358 c          194 g          646 t
ORIGIN      
        1 ttcctctttt ataagccaat gccgcaccca ccaatgcaat ggcctctgtt aattgatcta
       61 gcccttctat ggtgtgttta atttcaattt gatttctcat tacttttcct cctcctttaa
      121 ccctaaataa cactccatta attctttcat tctaacttcg tttttctcta ataccttcct
      181 tgcatctctg ccttctctga ttgttcttct tagctctgta ttttcttctg ataaatattt
      241 aatttctgat tcaatcttct cttttgaact ctcctctaat ttttctaatt ttaatttata
      301 gggcattgat tatttttctc ctttcgtggt atacttctaa taagaattat ttatttatga
      361 gaacctattt cacttggtcg gtggtaggtt ctttttctat tactacagta atatccctcc
      421 cgtcattcac tattactgcg attccatgct tgtgggcatc aattgcctgt cctagagtta
      481 tttctttcgc cgaattctcg tatgttatgc tcatccttca acacctccta atattttttc
      541 agtatgtcat ggattactag ccttgcccta gtgactttag ctgaatttat gtgatttggg
      601 atatacatag aatcaaactt ttttaaggtt tctagcaccc taatacaaga gttcacatca
      661 ttaataaatt gttctttttg ttcctttttt cttttctctc tttcttcccg agtcatacta
      721 taaattctcc tttcctattt ctttttttta taaacaatac agccattaca gtatggtctt
      781 tcgccccctc ctcaattcgt ctatgaaagc tataaacact gctccaaaaa acggtatccc
      841 ccatagccag cttgtcctgg ctagtgtcca tagatacaat ctaaaatcac ccaattttac
      901 atcttcctct cctctcctat atactcttta agcagttttg gactaatatg gtatgtccat
      961 ctactagaca tttttactgc tgttcctatg ggaagttttc ccctttggag tcctatcctt
     1021 atgaattgtt cagatacccc aagaatttcc gctgcttctt taactgatat gtttttttcc
     1081 atccatatca tctccctgct caatgattgg cagtatgcca ttttccttta acagttcgta
     1141 taaaaataat ctcccttttt gtgtccactt agtgttcatc tttatgtctg gagtcccatc
     1201 ccttctaact atctctactg tttcggaatg ggtatatccc tttccatgaa tatttgaaat
     1261 ccagataaat atcagtattg aaactaatac tgctataatc gaggaggtta taactaaacc
     1321 gatacgcaac catttatttt caaatttaag cttagctata atacctctat ctgttattct
     1381 aaaagcttgt ctttctttat tcacgccttt cacctctctt ttttatatta gggtctccat
     1441 ttctatgtgg tttgttcttc cacaaaagta tctaacggaa tctccaaggc ctggcaaatt
     1501 aaaaaatatt cttctgctaa tatttttctg tttccattta aagcagcatt taaagttgaa
     1561 attggaatcc ctgttttatt tgat
//
LOCUS       DATGSI010000525         1700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_557144, whole genome shotgun sequence.
ACCESSION   DATGSI010000525 DATGSI010000000
VERSION     DATGSI010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557144"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..374)
                     /gene="atpE"
                     /locus_tag="VK031_08160"
     CDS             complement(90..374)
                     /gene="atpE"
                     /locus_tag="VK031_08160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HLS53932.1"
                     /translation="MFEGITSEAFILGCSAIGAGLAMIAGIGPGIGQGHAAGHAASAV
                     GRQPEAQADITRTMLLGQAVAETTGIYGLVIAIILLFVRPLLTAYINLGL"
     gene            complement(402..1070)
                     /gene="atpB"
                     /locus_tag="VK031_08165"
     CDS             complement(402..1070)
                     /gene="atpB"
                     /locus_tag="VK031_08165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HLS53933.1"
                     /translation="MDILLTIGGREIMIHDTIVNTILVVIALSIFAIIINKKIKEADM
                     EETPTGLLNLVEILVEAINNLVDSTMGSKNMRFAPYIFSLMCFLAVSNLLGLIGLTPP
                     TSDYSVTFSLALITFSLTQIMAFKTARGLGGYLKGFTEPMAFLTPLNVIGELANPISL
                     SFRLFGNIMSGGIIMGLLYSALGYFAPIVTPVLHGYFDVFSGLLQTFIFGMLTMIFIG
                     GATE"
     gene            complement(1132..1503)
                     /locus_tag="VK031_08170"
     CDS             complement(1132..1503)
                     /locus_tag="VK031_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HLS53934.1"
                     /translation="MNDDLIFKIYKRTSIISLLIMGFSFFIFKQPKPIVLGYLFGVLI
                     SMLGFKLLDITINKAVKMNPKRAQAYTTFHYFLRYLIYFIVLSISAIANYLNFLATAL
                     GLLMIKFVIIGSTIFDKKFVE"
     gene            complement(1508..>1700)
                     /locus_tag="VK031_08175"
     CDS             complement(1508..>1700)
                     /locus_tag="VK031_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HLS53935.1"
                     /translation="LVLISQIGISIITPILLGVFIGQFVDKWLKTEGIFVIIFIILGT
                     GAGFLNLFKLTGAWKDKGK"
BASE COUNT          553 a          317 c          259 g          571 t
ORIGIN      
        1 tgtaataatc attgaaggta tttcaggaag tgctaatact cttaattcca taaactgacc
       61 tccttaggtt gggttttatc atattttaat tatagcccta ggttaatata tgctgtaagt
      121 agtggtctta caaatagtag aataatagct attactaaac cataaatacc tgtagtctcc
      181 gcaactgcct gacctaataa catggtccta gttatatctg cttgcgcttc tggttgccta
      241 cctaccgctg aggctgcatg gcctgctgca tgaccctgac caatcccagg tcctatacct
      301 gctatcattg ctaaacctgc tcctattgct gaacatccta atatgaaagc ttcacttgtt
      361 attccttcaa acattatatt tcctccttaa attttttatt tttattctgt tgctccccca
      421 ataaatatca tggttagcat accaaatatg aaggtttgca acaaacctga aaatacatca
      481 aagtaaccgt gtaatactgg tgttactata ggtgcaaaat aacctaatgc actatatagt
      541 aaacccataa ttatgcctcc actcattatg tttccaaata aacggaagga taatgagatt
      601 ggatttgcca actcccctat tacatttaaa ggagttaaaa atgccatagg ttctgtaaaa
      661 ccttttaaat aacctccaag gcccctggct gttttaaagg ccataatttg ggtgaggcta
      721 aaggttatta aggccaatga aaaagtgaca ctataatcgg aagttggtgg tgtaagtcct
      781 attaatccca gtaggtttga aactgctaag aaacacatga gactgaatat ataaggtgcg
      841 aatctcatgt tcttagatcc catagttgag tctactaagt tattaatagc ttctaccaga
      901 atttccacca aatttaatag tcctgttggt gtttcttcca tatctgcttc tttgattttt
      961 ttgttgatta taatggcaaa tatggataag gcgattacca ctaggatggt attaacaatg
     1021 gtatcatgaa tcataatttc tctgccacct attgttagta gtatatccaa gatattcctc
     1081 cttccttacg aaatggtttt attagtatgc cctagaattg atttataata gttattccac
     1141 aaatttcttg tcgaaaattg tagagcctat tataacaaat ttgatcatca atagaccaag
     1201 tgctgttgcc agaaaattta aataattcgc aatagcggaa attgacaaga caataaaata
     1261 tatcaagtaa cgtagaaaat aatgaaaggt tgtgtatgcc tgtgctcttt tagggttcat
     1321 tttcactgct ttatttattg ttatatctaa cagcttaaat cctagcatac taatcaatac
     1381 accaaacaga tatcctaaaa ctataggttt tggttgttta aatataaaga agctaaaacc
     1441 cataattaac aggctgataa ttgaagttct cttgtaaatt ttaaatatta aatcatcatt
     1501 cactgactca cttccccttg tctttccagg ccccagttaa tttaaataag tttaaaaaac
     1561 ctgcacctgt tcctaaaata ataaatataa ttacaaagat accttctgtt ttaagccatt
     1621 tatccacaaa ttggcctata aaaacaccta gtaatattgg cgttattatt gatattccaa
     1681 tctgtgagat taaaaccagg
//
LOCUS       DATGSI010000526         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_557241, whole genome shotgun sequence.
ACCESSION   DATGSI010000526 DATGSI010000000
VERSION     DATGSI010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557241"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VK031_08180"
     CDS             <1..1056
                     /locus_tag="VK031_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HLS53936.1"
                     /translation="KMRKRTLSLFLIVVLLSSVILTACQGGGQDKDENSSSKNTAGAP
                     VHLTFATQEVGTGAYQYASAITSAFTKALPEGSNVDLTTESPGGVGAPIVIENGQADI
                     IMSNAGPAKWAAEDGILGNPPTENVRAIAGGLGRDFVNVLFTQEFVDKTGITTVEDIV
                     EKEYPVRIAVKKIGTLGHLAFEKLFEALGVTVEDIESWGGKVDLLAGDTVKTYLQDGK
                     ADITVDHVAAGQANTTELCMTTEMHFPQLADETLNKLQEMGFAPTTIEADTWKGQTTE
                     IQSVGSQQVILVPEDMDDEIAYALTKGLVENREDLAAQISALSHFNPEEAWKPEYLGV
                     EIHPGAKKYFEEAGYMN"
     gene            1107..2996
                     /locus_tag="VK031_08185"
     CDS             1107..2996
                     /locus_tag="VK031_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HLS53937.1"
                     /translation="MTRLNNRDKLLYSIMAIFLLFQLYIALIKPLDPMLQSPIHLLLA
                     LLVVFLMKPLKKDNMAAKIFEFICYMAIIFLFYYTFSQTDRLVNRVQYVDQVFTIDYI
                     ATAVTIFILLEAVRRTLGSVLYGLIIFFIIYDWFGMYFPGILAFKGTNWRSFTEVMTM
                     GSSGVYGAPLYTSVTSLFYFILFGALFSTCGGGQLLIDIGMKTSSKGSGGPAKAAVIS
                     SGLMGMISGSAVANVSTTGVLTIPMMKEVGYEPHEAGAIEAVASTGGQIMPPIMGIGA
                     FIMAEMLGVPYKDVAAAAIIPALAYFISILLLVTFLAKRRDAGDIEEISTEIKIEPIL
                     PRLYLLLPAIILVYYIIQGKSLMRSGMMGIFAIIAVNFAGLLIQDGKNYIPIKSIWES
                     IKDGIQQAANIAIPTAACGIIIGIVIQSGLASRFADLIASLGTENLLVALLIAMLGCM
                     ILGMALPTVAAYLVSNILFVPVLIRLGIGQLPANMFVFYFGIMAQITPPVCLASYTAA
                     GIAGADSMKTGVTGFVYALVAFLVPFIFIYQPAILLIGSVKEIIMASFVLLLGTVYLA
                     AAMAGYLIIPLNKVKRGILLAGALLLIMPEPITDIIGLVMGTSILVLNLIISRKQKLG
                     DSVEG"
     gene            2986..>3537
                     /locus_tag="VK031_08190"
     CDS             2986..>3537
                     /locus_tag="VK031_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HLS53938.1"
                     /translation="MKVKEILSYKILIIGAGSSALTLASKLVKEGENDIGIYSLAHGA
                     SPEIAGINFLTEDTIEERELYVEDMLKSGYRLNEIEHVKRMVGESFETLGFMEEIGVE
                     FDKDGRTYKKRHLSGHSKPRTLYNSKEFIGTNILKKQRNYLKDAGVNVNLRHECIDVR
                     EVNGRKSALFIKDGELIEIYADII"
BASE COUNT         1180 a          525 c          771 g         1061 t
ORIGIN      
        1 aaaatgagaa aaagaacttt atcattattt ttaatagtag tgttgttatc atcagttatc
       61 ttaacggctt gccaaggtgg cgggcaagat aaagatgaaa attcttcatc taagaataca
      121 gcaggagcgc cggtgcattt aacatttgca actcaggaag taggaacagg agcttatcaa
      181 tatgcatcag ccatcacatc agcttttaca aaggccttgc cggaaggatc taatgtagac
      241 ttaaccacag aatcaccggg aggagtggga gctccaattg ttatagaaaa tgggcaggct
      301 gatattatta tgagtaatgc tggaccagcc aaatgggcag cagaagatgg aatactaggt
      361 aatcctccaa cagaaaatgt aagagcaatt gcaggaggac tgggacgaga ctttgtaaac
      421 gttctattta cacaagaatt tgtagacaag actggtataa ccacagttga agatatagtt
      481 gaaaaagaat atcctgtaag gatagctgtt aaaaaaatag gtacactagg acacctagct
      541 tttgaaaagt tatttgaagc cttaggggtt acagtagaag atatagaaag ctggggcggt
      601 aaggtagatc tactggctgg agatactgta aaaacttatt tgcaagacgg taaggcagat
      661 ataacagtgg atcacgttgc agccggacaa gcaaatacta ctgagttgtg catgactact
      721 gaaatgcatt ttccacagtt agctgatgaa accctaaata agcttcaaga aatgggtttt
      781 gcccctacaa ctatagaagc tgatacatgg aaagggcaaa ccacagagat tcaatcagta
      841 ggttcacaac aagttatttt ggtacctgaa gacatggatg atgaaatagc ttatgcattg
      901 actaagggat tagtagaaaa tagagaagat ttggcggcac aaatctcagc attatctcac
      961 tttaatcccg aggaagcctg gaaaccagaa tacttaggtg tagagattca tccaggtgcc
     1021 aaaaaatatt ttgaagaggc aggatatatg aattagttac ttagcaggct taggcctgct
     1081 aagattatga aaaggagcta ggaattatga caagattaaa taatagagat aaattattat
     1141 atagtattat ggctatattt ctgttgtttc aactatatat agccttaata aaacctttag
     1201 accctatgtt acaaagtcct attcacttat tactggcatt gcttgtagta tttctgatga
     1261 aacctttaaa gaaggataat atggcggcta agatatttga atttatctgt tatatggcga
     1321 taatatttct attttattat acatttagcc aaacagatag actggttaat agggtccaat
     1381 atgtggatca agtatttacg attgattata tagcaacggc ggtaacaata ttcatattat
     1441 tggaagctgt taggagaacc ttaggttctg tgctgtatgg gttaattata ttctttatca
     1501 tatatgattg gtttggaatg tattttcctg ggattttagc ctttaaggga actaactgga
     1561 gaagttttac agaggttatg accatgggtt cttcaggagt atatggagcc cccttatata
     1621 catcagtaac atctcttttt tactttatat tatttggtgc tttgttttcc acatgtggag
     1681 gtggacaact actaatagat atcggaatga aaacatcaag caaaggttca ggagggcctg
     1741 ctaaggcggc agtaatatct tctggtttaa tggggatgat atcagggtct gcagtagcaa
     1801 atgtttcaac tacaggagta ttgacaatac ctatgatgaa agaagtagga tatgaacccc
     1861 atgaggctgg agccatagag gctgtggcat ctactggtgg acaaattatg ccacctatta
     1921 tgggaatcgg tgcctttatt atggcagaaa tgcttggagt tccctataag gatgtggcag
     1981 cagcagccat cattccagca ttggcatatt ttatttccat cttattgcta gtaaccttct
     2041 tagcaaaaag aagggatgct ggggatatag aagaaatatc tacagaaatt aaaattgagc
     2101 caattttacc aagactttat cttttattac cagccattat attggtttac tatattatcc
     2161 agggtaagtc attaatgcgt agtggaatga tgggaatttt tgcaattatt gcagttaact
     2221 ttgcagggct tttaattcag gatggaaaaa attacattcc tattaagagt atatgggaat
     2281 ctattaaaga tggtattcaa caggcagcca acatagctat tcctacagca gcatgtggaa
     2341 ttataatagg aatagtaatt caatcaggcc tagcctctag gtttgcagac ttaatagcca
     2401 gtttaggaac agaaaatctt cttgttgcct tactaatagc catgttaggt tgcatgatcc
     2461 taggaatggc actacctacg gtagctgctt acttagtttc aaatatacta tttgtgccag
     2521 tattaataag attaggcatt ggccaactac cagcaaatat gtttgtattt tattttggaa
     2581 taatggcaca aattactcca cctgtgtgct tagcatctta tacagctgca gggatagcag
     2641 gtgcagattc tatgaagact ggagtaacag gatttgttta tgccctggtt gcattcctgg
     2701 taccattcat attcatttat cagccagcta ttttattaat aggtagcgta aaagagatta
     2761 ttatggcaag ttttgtgttg ttattaggaa ctgtttattt agcggcagct atggcagggt
     2821 acctaattat acctttaaac aaggttaaaa gaggaatttt actggctgga gctttgctct
     2881 taattatgcc agagcctata acagacatta ttggtcttgt gatggggaca agtatattag
     2941 tattaaactt aataatttct agaaaacaaa aattaggtga tagtgttgaa ggttaaagaa
     3001 atattatctt ataaaatttt aatcataggg gcagggtcca gtgctctgac cctagcttct
     3061 aaactagtaa aagaagggga gaatgatata ggtatatact ccctggccca tggggctagt
     3121 ccagaaatag ctgggattaa ctttttaaca gaagatacaa tagaagagag agaactttat
     3181 gtagaagata tgttaaagtc tggatataga ttaaatgaga tagaacatgt aaaaaggatg
     3241 gttggggaaa gctttgaaac actaggcttt atggaggaaa ttggtgtaga gtttgataaa
     3301 gatggccgca catataaaaa gagacacttg tctggccact caaaaccaag aaccctttat
     3361 aattctaagg aatttatagg cacaaacatt ttaaaaaagc aaagaaacta tttaaaggat
     3421 gcaggtgtaa acgtaaattt aaggcatgag tgtattgatg ttagagaggt taatggcagg
     3481 aaaagtgcct tatttataaa agacggtgaa ttaatagaaa tatatgcaga tataatt
//
LOCUS       DATGSI010000527         1033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_557253, whole genome shotgun sequence.
ACCESSION   DATGSI010000527 DATGSI010000000
VERSION     DATGSI010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557253"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1033)
                     /locus_tag="VK031_08195"
     CDS             complement(<1..>1033)
                     /locus_tag="VK031_08195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLS53939.1"
                     /translation="QVSSLNKIVSDLDLNGNAYGWIINEKGTIVAHHDQDIAFQRSIK
                     EYIESDEALIDKIYENKTGVINYTGADGNKMLAFFTGIKYSPNWKFIVSVPEKEIYKK
                     VNELRYTMALVFVLGLSSIIVFAAIYSRSLSKPIMDLKNVFEKAANGNFEVVANEAIP
                     NELGNAATSFNRMLGQIKELTYEDLVTHSYNYSGFLYELPSKKELLRKKGQIVGISII
                     SIDDFKRINSIYGYEMGDEVLRLFTNRLKEFIDKEELVARFLGDEFILLLGAETRGEL
                     ECKVKDLWKISSTELIVGGNEFVLKTSVGASIKEDDIYSIEEIINQATVAKLIAKKAG
                     GNSCKFYDEE"
BASE COUNT          317 a          224 c          107 g          385 t
ORIGIN      
        1 actcttcatc ataaaactta cagctgttac caccagcctt tttagctatt aatttggcaa
       61 cagttgcctg gttgattatt tcctcaattg aatatatatc atcttctttt atgctggctc
      121 ctacactggt cttcagaaca aattcatttc ctcctactat taactctgta ctactaatct
      181 tccataaatc cttaacctta cattccaatt ctccccttgt ctcagctccc aagagcaaaa
      241 taaattcatc tcctaaaaat cttgccacca gctcttcctt atctataaat tccttcagcc
      301 tatttgtaaa taatcttaaa acttcgtcac ccatttcata tccatatata ctatttatcc
      361 ttttaaaatc atctatagaa attattgata tacctactat ttgtcctttc ttgcgcaaca
      421 actccttctt tgaaggtaat tcatataaaa aaccgctata gttataggag tgggtaacta
      481 aatcctcata ggttaattct ttaatttggc ctaacatcct attaaagctg gtagcagcat
      541 tacctaattc attaggtata gcttcattag ccactacttc aaaatttcca ttggctgctt
      601 tttcaaaaac attttttaaa tccataatag gttttgacag gctccttgaa tatatagcag
      661 caaaaacaat tatactactt agcccaagta caaatactaa agccatggta tatctaagct
      721 cattaacctt tttatatatt tctttttccg gaacagatac tataaacttc caattgggag
      781 aatatttaat tccagtaaaa aaagctagca ttttattgcc atctgctcct gtgtaattta
      841 ttacgcctgt tttattttca tagattttat caattaaagc ttcatcactt tctatatatt
      901 ccttaatgct cctttgaaaa gctatatcct gatcatgatg ggctactatg gttccttttt
      961 catttataat ccaaccataa gcatttccat ttaaatctaa atcacttact atcttattta
     1021 aggaagatac ctg
//
LOCUS       DATGSI010000528         1667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_459741, whole genome shotgun sequence.
ACCESSION   DATGSI010000528 DATGSI010000000
VERSION     DATGSI010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459741"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VK031_08200"
     CDS             complement(<1..328)
                     /locus_tag="VK031_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004818266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HLS53940.1"
                     /translation="MGLKKIIDKLKKKYISNKVAWADVPEFSPSNLVRKRIIFSGRVQ
                     NVGFRYEVFNMANRLELTGWVKNLADGNVESEIQGTEEKILFLTEHMKSLKRAQVEDV
                     TIEDLEV"
     gene            complement(596..>1667)
                     /locus_tag="VK031_08205"
     CDS             complement(596..>1667)
                     /locus_tag="VK031_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HLS53941.1"
                     /translation="VRGRNKQKFSEACRPILKDKVDSFLGKVIDLIAVFALLAGTATT
                     FSMATPLLSAAISRVFGIKPGIRLTIIILLVIALVYTLTVLFGIKGISKLANLCTYLF
                     GILLVYFLFGGGEIVYIIETGISSIGNLVQNFISMATWLDPLRTSGDGGLGFVQNWTI
                     FYWAYWMAWCVATPFFIGVISEGRTIRNVITGGYLWGVAGTFTSFIVFGNFGLSQQMK
                     YGIDIAGALKAGQEPSHVILSIFDRLPLTNLGILLLVITMIAFYATTFDALTMVISYY
                     SYKRLDVSQGPDKKVRVFWAILFIILPIALIFSESSLKNLQTVSIIAAFPIGLVVLLI
                     VGSFFKDAKTYIDNYENSRSVE"
BASE COUNT          539 a          351 c          229 g          548 t
ORIGIN      
        1 ttacttctaa atcctcaatg gttacatctt ccacttgagc tctttttaaa gatttcatat
       61 gttctgttaa gaaaaggatt ttttcttctg ttccctgtat ttccgattcc acattgccat
      121 ctgccaggtt tttaacccag cctgttagct ctaatctatt ggccatatta aatacctcgt
      181 atctgaagcc tacattttga acccttcctg aaaatatgat tcttttccta accagattag
      241 aagggctaaa ttctggtaca tcagcccagg caaccttatt tgatatatat tttttcttaa
      301 gtttatcaat aatcttttta agacccatat actaacctcc aagattatct tttatttagt
      361 tctaatctaa atttctccaa tggctagaac ctgctgtcat gcgcatctag ctagccttaa
      421 tcctattcta tcctaggata tacttaagtt caattaaaga acttcttgct atatataaag
      481 atagcttagg ttttaagctt atttaactat aatcccccag tttagtatgc atattctaca
      541 atccattaca ttccttccct cgcttcacca aaaccttatt catattcttt ggctattatt
      601 ctacacttct tgaattctca taattatcta tataagtctt tgcatcttta aaaaagcttc
      661 ctacaatcaa caatacaaca agtccaattg gaaaagcagc tattatggaa acagtttgta
      721 gattcttaag cgagctttcg gaaaatatta atgcaattgg taaaataata aatagtatag
      781 cccaaaagac acgaaccttt ttgtcaggcc cctggctaac gtctaatctc ttgtatgaat
      841 aatatgaaat taccattgtt agtgcatcaa acgttgtggc ataaaaagct atcattgtta
      901 taaccaacaa taaaatccct agattggtta aaggaagcct atcaaatatg gataggataa
      961 catgtgatgg ctcctgccct gcctttagtg caccagctat atctattcca tatttcatct
     1021 gttgagaaag cccaaaattt ccaaaaacaa taaaagaggt aaaggtccct gctacccccc
     1081 ataaatatcc acctgtaata acattcctga tggttctacc ttcactgata acgccgataa
     1141 aaaaaggtgt tgctacacac caagccatcc aatatgccca gtaaaatata gtccaatttt
     1201 gaacaaatcc taagcctcca tctcctgatg tacgaagcgg atctagccag gtggccatac
     1261 tgataaaatt ttgaactaaa ttaccaattg aagatatacc ggtttcaatg atatatacaa
     1321 tctctccacc accaaataag aaatatacta gtaaaattcc aaataagtag gtgcatagat
     1381 ttgccaattt ggagattccc ttaattccaa ataaaacagt taaggtatat actaaggcaa
     1441 tcaccagtag gattattatg gtcaacctta tacctggttt tataccaaag actctactta
     1501 ttgcggctga taataaaggc gtggccatag agaaggtagt tgcagttcct gccaataatg
     1561 caaaaactgc tattaaatca attaccttgc ctaaaaagga gtctacttta tccttaagaa
     1621 ttggtctaca tgcttctgaa aatttctgct tatttcttcc tcttaca
//
LOCUS       DATGSI010000529         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_34081, whole genome shotgun sequence.
ACCESSION   DATGSI010000529 DATGSI010000000
VERSION     DATGSI010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34081"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VK031_08210"
     CDS             <1..447
                     /locus_tag="VK031_08210"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HLS53942.1"
                     /translation="KNYNMMIGERTAERLKHSIGCVYPMEEEKFESVKGRNLLTGLPI
                     NVDISSNDMLEAFESTIEEIVEAIHSTLERTPPELAADIGNRGIVMTGGGALIYGMDK
                     LLAEELGIDVHIAPNAMECVARGTGRSLRWINMLKDSDKTNFNFRR"
     gene            complement(981..1057)
                     /locus_tag="VK031_08215"
     tRNA            complement(981..1057)
                     /locus_tag="VK031_08215"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1021..1023),aa:Pro,seq:tgg)
     gene            1299..1763
                     /locus_tag="VK031_08220"
     CDS             1299..1763
                     /locus_tag="VK031_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HLS53943.1"
                     /translation="MGNFLASIKKELLVIFFSMVPVFELRGAIPLGISLGLSPLHSWV
                     LSVISNILMAGFLLKLLNPIMNYFEKTRLFKSTIGWVKTRSMKKAATIKKYSLIGLFF
                     FVAIPIPTTGVWTASVIASILNLDIKKSLISMGLGVVAAASIVLGLYYGIFI"
     regulatory      1823..1929
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2009..>2256
                     /locus_tag="VK031_08225"
     CDS             2009..>2256
                     /locus_tag="VK031_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009344844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HLS53944.1"
                     /translation="MEKWFFTSESVTEGHPDKVCDQISDAILDAILEKDPEARVACET
                     MATTGMVLVAGEITTDCYVDIPKIVRETVEEVGYVRAK"
BASE COUNT          781 a          313 c          436 g          726 t
ORIGIN      
        1 aagaattata atatgatgat aggagagagg acggctgaaa ggctaaagca tagtataggc
       61 tgtgtatatc ctatggaaga agagaaattt gaaagtgtaa agggtaggaa ccttttaaca
      121 ggccttccca ttaatgtaga tataagcagt aatgatatgc ttgaggcttt tgaaagtact
      181 attgaggaga tcgttgaagc tattcactct actttggaaa gaacgccacc tgaactggcc
      241 gctgatatag gaaatagggg tatagttatg accggtgggg gagctttaat ttacggaatg
      301 gacaagctat tggcagaaga attaggtata gatgtacata tagcacctaa tgccatggaa
      361 tgtgtagcca gaggtacagg aagatcttta agatggataa atatgttaaa agatagcgat
      421 aaaacaaatt ttaactttag aagataaaaa agcttaggaa taatgtccta agttttttat
      481 ttttcataaa accagtatta aatttgagtt aaaataagag gcctataaac cattagaata
      541 aaaatcaaaa ccatataata aaataccaac tgaatcagtt cagttggtgt tttcaattcc
      601 ttataggtag tctaataacc cattgtatgc cttgatatta ctatattttt atttttctgt
      661 caaatggtgt tttctcatat ttttatatgc ttaaatatat caaaattgga ttctgtagtt
      721 gtaagtaaga ggtctatggt cctgttatat aaaaatgtcg acctccaggg gtttttgcac
      781 tattgggggt cggcgacaaa aaatatataa actacctagg tgtttatata ggtatataat
      841 aggtaatata aaactcaaaa atgttctcga aataaaatgt gtattgatcc taatggtaat
      901 atccattaga ttctatgatt aatacttttt aataaaaata aaataggaat ggttaaataa
      961 ccattcctat tagtaaatgt tggtcggggt gagaggattt gaacccccgg ccccatggtc
     1021 ccaaaccacg tgcgctacca aactgcgcta caccccgtca tttccaacga ctcttatatt
     1081 ctaatatata aatttactta atgcaatatt aaaatatacc ctttaaacat ctataaatgc
     1141 aattaaataa gaataagcca aatatataga gacttatcta agtatatctc gctatttcta
     1201 ggtatttatt taaaaaacct caaaagccat taattttctt gcttaatatt gtattctgcc
     1261 atataataat tttgataata agcgagaaag gatagattat gggtaacttt ttggcgagta
     1321 ttaagaaaga attattggta atattttttt ctatggtgcc agtatttgag cttagaggtg
     1381 ccattccctt agggatatct ttaggattaa gtcctttaca tagttgggta ttaagtgtta
     1441 ttagcaatat attaatggct ggttttttgt taaaactatt aaatcctata atgaactact
     1501 ttgaaaaaac taggcttttc aaaagcacca ttggctgggt taaaactaga tcaatgaaaa
     1561 aagcagctac catcaaaaaa tatagtttaa taggcctatt tttcttcgtt gctattccaa
     1621 ttccgaccac cggggtttgg actgcctctg tgattgccag tattttaaat ttagatataa
     1681 aaaaatcatt aatttctatg gggcttggag tagtggcagc tgctagcata gtattaggcc
     1741 tatattatgg tatatttata tagaacatgt aactatttga ctatatatat aatatatgct
     1801 aacatgggaa taacttaata ataccttatt aagagtgatg gagggaatag gcccaatgaa
     1861 gtccggcaac cttgtattat atacaaaggt gctaaatcct acagaatgaa gaattctgaa
     1921 agatgaggtt actggaattg gccttttctt tttagaatgg gcttttttta tggcctcaac
     1981 aaataattta cacaaggaga gataagtgat ggagaaatgg ttttttactt ctgaatcagt
     2041 aacagaaggg catccggaca aggtttgtga tcaaatatcc gacgccatat tggatgccat
     2101 attggaaaaa gatccagagg ccagagtagc ttgtgaaaca atggctacta caggcatggt
     2161 attagtagcc ggagaaatta ctacagattg ttatgttgat attccaaaga ttgtaaggga
     2221 aactgtagaa gaggtaggct atgttagggc taaata
//
LOCUS       DATGSI010000530         1360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_226690, whole genome shotgun sequence.
ACCESSION   DATGSI010000530 DATGSI010000000
VERSION     DATGSI010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226690"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..>1360
                     /locus_tag="VK031_08230"
     CDS             312..>1360
                     /locus_tag="VK031_08230"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HLS53945.1"
                     /translation="MPTKYIFVTGGVVSSLGKGITAASLGQLLKSRGLKVTMQKFDPY
                     INVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDVSLNKNCSVTTGKVYWSVLNK
                     ERKGEYDGRTVQVIPHITNEIKERIIRASKDRDIDIVITEIGGTVGDIESLPFLEAIR
                     QIKYDVGKENVMYIHVTLVPYLKKAGELKTKPTQHSVKELRGLGIQPDVLICRAEKPL
                     GEELKDKIALFCDLDPSEVIESTDANNIYELPILMEEQGLAKLTIEHLNLKCGELKLD
                     HWYELLDKMNRISGKVKIALVGKYVELRDAYISVVESLQHGGIENSVDVEIKWIQAED
                     LNNSNCEELLKDMDG"
BASE COUNT          486 a          166 c          299 g          409 t
ORIGIN      
        1 ccaaatttga gggttatttt tatgtgaaat atttggcctt ttagcctata atttgtgtta
       61 ttataggtac taaggcagat aatacaaata ggatgattaa aatccaggcc attttttgtt
      121 tcatatggat caccttctag attattatac tcttattata gttgatttaa ttaatattgc
      181 aattatttat taagtttgaa ttatgcatag gtttagcttt gtagattatg gtggattgga
      241 tttgattgac taataatttt tattaatata gaatgtaata gttgatttag aaacaaaagg
      301 agaggaatag gatgccaact aagtatattt ttgtaactgg tggagtagtg tcttccttag
      361 gtaaaggaat tacagctgcc agcttgggcc aacttctaaa aagtagaggt ttaaaggtta
      421 ctatgcaaaa atttgaccct tatataaatg ttgatccagg aaccatgagt ccatatcaac
      481 atggagaggt ctttgtaact gatgatgggg cagaaacaga tttagacctt ggccattatg
      541 aaagatttat agatgttagt ttaaataaaa attgtagtgt aaccacagga aaggtgtatt
      601 ggtcagtact taacaaggaa agaaagggag aatatgacgg aagaactgtg caagtaatcc
      661 ctcatattac caacgaaatc aaggaaagaa ttatcagagc ctcaaaggat agagatatag
      721 atattgttat tacagaaatt ggagggactg taggtgacat agagtccttg ccctttttag
      781 aggccataag acagattaaa tatgatgtgg ggaaagaaaa tgttatgtat atccatgtaa
      841 ctttagtccc atatctaaaa aaggctggtg agctaaaaac taagccaacc cagcatagtg
      901 taaaagagct taggggccta ggaattcagc cagatgtact aatatgtagg gctgaaaaac
      961 ctttaggaga agaattgaaa gataaaattg ccctattctg tgacttagat ccatcagaag
     1021 ttatagaaag cacagatgcc aacaacattt atgagctacc aatcctaatg gaagagcagg
     1081 gattggcaaa gttgaccata gagcatttaa atcttaaatg cggagaattg aaattagatc
     1141 attggtatga acttttagat aagatgaaca ggataagcgg taaggtaaaa attgccctag
     1201 tgggaaaata tgtggagcta agggatgcct atatttcagt ggttgaatct ttacagcatg
     1261 gtggtattga aaatagtgtg gatgtagaaa ttaaatggat ccaggcagaa gacttaaata
     1321 atagtaattg tgaagaacta ttaaaagata tggacgggat
//
LOCUS       DATGSI010000531         1286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_698634, whole genome shotgun sequence.
ACCESSION   DATGSI010000531 DATGSI010000000
VERSION     DATGSI010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698634"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VK031_08235"
     CDS             <1..487
                     /locus_tag="VK031_08235"
                     /inference="COORDINATES: protein motif:HMM:NF006132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLS53946.1"
                     /translation="LFDLDEEGIRFVFDLNFLGTFIPSQEFVKDMVGREGCTIINISS
                     MNAYTPLTKIPAYSAAKSAISNFTQWLAVHLSKVNIRVNGIAPGFFLTSQNEALLLNP
                     DGTYTDRSEKILAGTPMGRFGKAEELAGVLLFLVSQEASGFVNGTVIPVDGGFSAYSG
                     V"
     gene            complement(575..>1286)
                     /locus_tag="VK031_08240"
     CDS             complement(575..>1286)
                     /locus_tag="VK031_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLS53947.1"
                     /translation="PEYSKLFSDTFGVTSKELLSKYPTPEDMLSIDTNTLTELLSKAS
                     KGRFGISKANEIQESVSSTFGVNFAKDAFAFQIKQIISQINFIEDQLKELETEISTLL
                     HKTNAVITTITGIGDVLGAIIIGEIGDISRFESAPQLVAYAGLDVAVKQSGDFVGTQT
                     KISKRGSPYLRRAIWLAATVAAFKDPALSIYYQSLKARGKHHLTAVGYVKFRQMIIPA
                     IQFYWVLRNSVVLFLTRD"
BASE COUNT          415 a          218 c          256 g          397 t
ORIGIN      
        1 cttatttgat ctggatgagg agggcataag gtttgtattt gacctgaact ttttaggcac
       61 ctttatacca tctcaagagt ttgtaaagga catggtaggc cgtgaaggct gtactataat
      121 taatatttct tcaatgaacg cctatacacc attgaccaaa atccctgctt attcagcagc
      181 taaatcggca atttcaaact ttacccagtg gttggctgtt cacctatcta aggtaaatat
      241 tagagtaaat gggatagctc ctggtttctt tttaactagc cagaatgagg cccttttact
      301 aaatcctgat ggaacctata cagatagatc tgagaaaata ttagcaggta ctcccatggg
      361 caggtttggg aaggctgaag agttggcagg agttctacta ttcctagtaa gccaagaggc
      421 ttctggcttc gtaaatggaa cagtgatacc agtggatgga ggattctcag cttattcagg
      481 tgtttaaaat gtagtaagaa ttaagggcta attttataaa ggcaaattaa ataaaattag
      541 cccttttata gataagtttc tatgaaaaaa gtatttaatc cctagtcaga aatagaacaa
      601 cagaattcct gagaacccaa tagaactgaa tagctgggat aatcatctgc cgaaatttta
      661 cataacctac agctgttaaa tgatgctttc cacgtgcttt aagtgactga taatatatag
      721 ataatgctgg atctttaaag gctgcaacag tagcagctaa ccaaattgct ctacgaagat
      781 atggagaacc acgttttgaa atcttagttt gagttccaac aaaatctcca gattgtttta
      841 cagcgacatc aagtccagca taagcaacta gctgtggtgc agattcaaaa cgggaaatat
      901 cgccaatttc tccgatgatt atggctccaa gaacatctcc aattccagtg atcgtagtga
      961 ttactgcatt ggtcttatga agcaaggttg aaatttcagt ttcaagttct ttaagttgat
     1021 cttcgataaa gttaatttga gaaataattt gtttaatttg aaatgcaaag gcatcctttg
     1081 caaagtttac tccgaaggta ctagaaacag actcttgtat ttcattagct tttgagatac
     1141 caaatcgacc tttacttgct tttgataaaa gctctgtaag agtgtttgta tctatggata
     1201 acatatcttc aggagtagga tatttagaaa gaagttcttt agaagttaca ccaaaggtat
     1261 ctgaaaaaag cttagaatac tcgggg
//
LOCUS       DATGSI010000532         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_460079, whole genome shotgun sequence.
ACCESSION   DATGSI010000532 DATGSI010000000
VERSION     DATGSI010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460079"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VK031_08245"
     CDS             <1..535
                     /locus_tag="VK031_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HLS53948.1"
                     /translation="YKEVEENGALISEFPLGMPPLAYNFPRRNRIIAGLSLGIIVIEA
                     EKKSGSLITAHHALEQGKDVFALPGNINSIYSQGTNRLIRDGARPLLEIDDILEEIKI
                     LQDKISIQEKDRLNLDGLSKTEQDIVATLKEGPIHSDLIAHKTGLEISTVIGALTILE
                     MKGIVKELNNGIFTLSQ"
     gene            559..2646
                     /gene="topA"
                     /locus_tag="VK031_08250"
     CDS             559..2646
                     /gene="topA"
                     /locus_tag="VK031_08250"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HLS53949.1"
                     /translation="MSRKLVIVESPAKAKTIGKLLGRNYKVVASVGHIRDLPKSTLGI
                     DIENNYEPRYITIRGKGPVIKELKKEARKASKVYLATDPDREGEAISWHLAHILDIEE
                     DEKVRVGFNEITKEAVQKAIKNPRSLDSDLIDAQQARRVLDRLVGYKISPLLWKKIRK
                     GLSAGRVQSVATKLICDREKEIENFVPEEYWSIRSIFTKDNLDFEGNFIGKYENAKVK
                     KIKLNNEEKVNAILQELDREQFIVKDVRKGSKRRNPYPPYTTSTLQQDASRRLRFSIR
                     KTMTIAQQLYEGIDIGGEGTVGLITYMRTDSTRISNEAIGAAKNYIIENFGEEYSNGG
                     RGYGGKNKDSQDAHEAIRPTIGLRDPESIKDSLTNDQYKLYKLIWDRFVASQMSAAKY
                     DTLRVNIDNNGYLFRASGSKLIFPGFLKVYAIIDNKDKEMDLPELAEKDKLTVKDIVP
                     KQHFTQPPARYTEASLIKTMEELGIGRPSTYAPTIGTILARDYVELDRRSFRPTELGI
                     LVNDLLTEYFNEIINEEFTAQLEEDLDHIAEGKYYWKKVIDDFYTNFDKILKKAEEEI
                     DKIEIKDEVTDKVCEKCGKNMVIKHGRYGKFLACPGYPECRNTKPILDKIDVECPKCG
                     GDVVKKRSKRRRVFYGCSNYPDCDFVSWDEPVEEKCPECKGFMVLKKTRKEDTIRCSN
                     RECGYSKEVNKKD"
BASE COUNT         1077 a          381 c          624 g          749 t
ORIGIN      
        1 atacaaagaa gtagaagaaa acggggcctt aatttcagaa tttcctttgg gaatgccacc
       61 cttggcttat aattttccca gaagaaatag aataatagct ggcctatcat taggtataat
      121 agtaattgag gctgagaaaa aatctggttc cttaattaca gctcatcacg ctttagaaca
      181 ggggaaggat gtatttgccc tgccaggaaa cataaacagt atttacagcc aaggtaccaa
      241 caggctaata agggatgggg ccaggcctct tttagaaata gatgatattt tagaggagat
      301 taaaatcctg caagataaaa tttctataca agaaaaagat aggctgaacc ttgatggact
      361 aagcaagaca gaacaagata tagtggctac tttaaaggaa gggcctattc attctgattt
      421 aattgcacat aagacaggat tagaaatatc tactgtaata ggtgccttaa caattttgga
      481 aatgaagggt atagtaaagg agctaaataa tggaatcttt actcttagtc agtaagcaag
      541 aggtatggag gtgtggtaat gtcaagaaaa ctagtaattg tagagtcacc agctaaggct
      601 aaaactattg gaaaattatt agggagaaat tataaagtag tagcctcagt agggcatatt
      661 agagatctac ctaaaagtac tttaggtatt gatatagaaa acaattatga gccaagatat
      721 ataaccataa gaggtaaggg gccggtaata aaagaactaa agaaggaagc caggaaggca
      781 agcaaggttt atttggctac agacccggat agggagggtg aagccatatc ctggcatttg
      841 gcccatatcc tagatataga agaggatgaa aaggttcggg ttggtttcaa tgaaataacc
      901 aaggaggctg tgcaaaaagc tattaaaaac ccccgaagct tagacagcga tttaatcgac
      961 gcccagcagg caagaagagt cttagataga ttggtaggat ataaaatcag ccctctgctg
     1021 tggaaaaaaa ttagaaaggg cttaagtgct ggaagagtac aatcagtggc aactaaattg
     1081 atttgcgaca gagaaaaaga aatcgaaaac tttgttcctg aagaatattg gagtatcagg
     1141 tctattttta caaaggataa tctagacttt gaaggaaact ttattgggaa atatgaaaat
     1201 gcaaaagtaa aaaagattaa gctaaataat gaagagaaag taaatgccat acttcaagaa
     1261 ttagataggg aacaatttat agttaaggat gttagaaaag gcagcaaaag aaggaatccc
     1321 tacccaccat atacaactag tactttacag caggatgcat caagaaggtt aaggtttagc
     1381 attagaaaaa ccatgactat agcccaacag ttatatgaag gtatagatat aggaggagaa
     1441 ggaacagttg gtttaatcac ctatatgagg acagactcca ccagaatatc aaatgaagcc
     1501 attggtgcag caaaaaatta tattatagaa aacttcggtg aagaatattc aaatggtggc
     1561 aggggctatg gaggaaaaaa taaggactca caggatgcac atgaggccat aaggcccact
     1621 ataggattaa gggatccaga gtcaattaaa gattctttaa caaatgatca atataaactt
     1681 tataagctta tatgggatag atttgtagct tcccagatgt ctgcagcaaa atacgatacc
     1741 ttaagagtaa atatagataa taatggttat ttatttaggg catctggttc taaattaata
     1801 tttcctgggt ttttaaaagt atatgctata atagataata aggacaaaga aatggacctg
     1861 ccagaactgg ctgaaaagga taaactgact gttaaggata tagttcctaa gcaacacttt
     1921 actcagccac cggctaggta cactgaagca tctctaatta agaccatgga agagctaggt
     1981 attggcaggc ctagtactta tgcacctacc attggcacta tactggccag ggattatgta
     2041 gaattggata ggagaagttt tagacctact gagctgggta tattagttaa tgatttatta
     2101 actgaatatt ttaatgaaat aataaatgag gaatttacgg cacaattaga ggaagattta
     2161 gaccatatag cagaaggtaa atactattgg aagaaagtta ttgacgactt ttacactaac
     2221 tttgataaaa tattaaagaa agcagaagaa gagatagata aaatcgaaat taaagatgaa
     2281 gttactgata aagtttgtga aaaatgtggc aagaatatgg taattaaaca tgggcgctat
     2341 ggaaagtttt tagcttgccc aggttatcca gagtgtagga atacaaagcc tattttggac
     2401 aaaatagacg tagaatgtcc taaatgtggt ggcgatgttg taaaaaagag aagtaaaagg
     2461 cgtagggtgt tttatggttg ctctaactat cctgattgtg attttgtatc ctgggatgag
     2521 ccggtagaag aaaaatgtcc tgaatgtaag gggtttatgg ttcttaaaaa aaccaggaag
     2581 gaagatacta taaggtgcag caatagggaa tgtggatatt caaaagaagt aaacaagaag
     2641 gattaaaagt gctgtacttt ttccttcttt tgtagtattc tatatattag aatattcaga
     2701 attaacttta ttatgaaatt aaaattgcaa tatacaatta gataatagaa caaggaggat
     2761 ttatgtggaa agtttattaa agaagacaag acgattaaat aaaaccttac aaagttatgg
     2821 ctcacatcca g
//
LOCUS       DATGSI010000533         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_226736, whole genome shotgun sequence.
ACCESSION   DATGSI010000533 DATGSI010000000
VERSION     DATGSI010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226736"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VK031_08255"
     CDS             <1..220
                     /locus_tag="VK031_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLS53950.1"
                     /translation="TAGIQATKENSADIGMVSRQLNEFEREDLLYETIALDGIAIIVN
                     KENPIDNLNLEELKSIYTGDIISWDQLK"
     gene            330..1190
                     /gene="pstC"
                     /locus_tag="VK031_08260"
     CDS             330..1190
                     /gene="pstC"
                     /locus_tag="VK031_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HLS53951.1"
                     /translation="MDRKNYEKVMKFIFTLSALTSIVAIMLICIFIFAEGLPFIKNYG
                     LKLFILGNSWKPANSPPSFGILPMILGSLYITIGAIILGVPLGILTAIYMVQFANSRT
                     RKILRLGVNLMAGIPSIVYGFFALTFLVPIIRNIFGGTGMNILTASILLGIMILPTII
                     TISEAAIRAVPNSYYLSSKALGASHERTVAKVILPAAKSGILSSIILGIGRAVGETMA
                     VILVTGNQARIPNNLFQGVRTLTTNIVIEMAYASGIHRQALIATALILFVFILIINLC
                     FFIIKGRSLE"
     gene            1187..>1599
                     /locus_tag="VK031_08265"
     CDS             1187..>1599
                     /locus_tag="VK031_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004826602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HLS53952.1"
                     /translation="MNKLIKTIVRLAAFTSLFILIFIIAYILFKGIPHLNPALFKWEY
                     TSDNVSLLPSLITTILTIILTLIIAVPLGIFTALYLIEYADKDGRLVQLIRITTETLA
                     GIPSIIYGLFGMLFFVIRLNFQYSLLSGSLTMSIM"
BASE COUNT          508 a          255 c          256 g          580 t
ORIGIN      
        1 tactgcagga attcaagcta ctaaggaaaa tagtgcagat attggtatgg tctccaggca
       61 attaaatgaa tttgaaagag aagatttact ttatgaaacc atagccttag atggaattgc
      121 aatcatagtt aataaagaga accctattga taatttaaac ttagaggaac taaaatctat
      181 atacactggt gatattatta gctgggacca attaaagtag ccctttaacc tggctacttt
      241 ttacatagat tttacaagtc taataaggta tagtttacaa actattgata ttgtttttgt
      301 aaacattcta aaaaggagca cttataatta tggatagaaa aaattatgaa aaagttatga
      361 aatttatttt tactctctct gccttaactt caattgtggc aattatgttg atctgtatct
      421 ttatttttgc agaaggtcta ccttttatta aaaattacgg tttaaaatta tttatcttag
      481 gcaacagttg gaagcctgct aactctccac caagctttgg aatcttgcct atgatattag
      541 gatccttata tattactata ggtgcaataa ttttaggagt acccttaggt atattaacag
      601 ctatttatat ggtacaattt gctaatagca ggactcgtaa gattttaaga cttggcgtta
      661 atctaatggc agggataccc tcaattgtat acggtttttt tgctttaact tttctagtgc
      721 ctattattag gaatattttt ggtggaactg gtatgaatat acttactgca tctattcttt
      781 tgggaataat gattcttcct accattatta ctatatctga agcggctata agggcagtac
      841 caaacagcta ctacctatcc agtaaagcac taggagccag ccatgaaagg accgtagcaa
      901 aggtaatttt accagcagca aaatctggta tattatcttc tatcatactg ggtataggcc
      961 gtgctgtagg tgaaaccatg gctgttattt tagttactgg taatcaagcc aggatcccaa
     1021 ataacctttt tcaaggcgtt agaacactta ctacaaatat tgttattgaa atggcttatg
     1081 cttccggtat tcatagacaa gcccttatag ccactgctct tatactattt gtattcatat
     1141 taataataaa cctttgtttc tttataatta aagggaggtc cctagaatga ataaacttat
     1201 taaaactatt gttaggttag ctgcatttac aagccttttt atcttaattt ttataatagc
     1261 ttatatttta tttaagggta taccccatct aaaccctgca ttatttaaat gggagtatac
     1321 atctgataat gtttccctat taccatcatt aataacaact atactaacga ttattttaac
     1381 ccttattata gccgtccctt taggtatttt tacagccctt tatttaatag agtatgctga
     1441 taaggatggt aggttagttc agcttataag aatcactact gaaaccttag ctggtatacc
     1501 ttcaattata tatggcttat ttggtatgtt attctttgtt attagattaa attttcaata
     1561 ttcactttta tcaggtagct taaccatgtc cataatgat
//
LOCUS       DATGSI010000534         1252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_268492, whole genome shotgun sequence.
ACCESSION   DATGSI010000534 DATGSI010000000
VERSION     DATGSI010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268492"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1252)
                     /locus_tag="VK031_08270"
     CDS             complement(<1..>1252)
                     /locus_tag="VK031_08270"
                     /inference="COORDINATES: protein motif:HMM:NF017116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HLS53953.1"
                     /translation="PDKPRIRLIIPLNRPVLPDEYQAIARRVAYDIGIDMFDDTTYTP
                     VRLMYWPSTAKDGEYVFKHQKGSLLDADKILDSYFDWTDINEWPKSSREDNLISNKAK
                     RQEDPYEKKGFIGAFCRTYSISEVIEKYLADVYAPTTHEGRYTYKKGSTVGGLVVYED
                     KFAYSHHGTDPISEKLCNAFDLVRIHKFGGLDESDGVKENTPVNRLPSFTKMIDLASS
                     DEKVKITLGKERFENLDDEFEFGEVEVADLDWMEKLSYNKKGEIENTIQNALVVLEND
                     PRVKGRLFYDEFANRAIVKKGVPWTDMDDHDWSDMDDSGVRFFLENNYNITTAYKIED
                     AKNLAFDRNKSHPVREYLKSLEWDGRERIESLFIDYLGAEDNLYTRSVAKIQLVGAVA
                     RVMRPGVKFDTMPTLTGPQGIGKST"
BASE COUNT          368 a          283 c          165 g          436 t
ORIGIN      
        1 agtagatttt cctatacctt ggggcccggt taaagtaggc atagtatcaa atttaactcc
       61 tggcctcatt actctagcca cagccccaac aagttggatc ttagctacac ttcgtgtata
      121 caaattatcc tcagccccta aataatcaat aaacaatgat tctattcttt ccctaccatc
      181 ccactcaagt gatttaagat actccctaac aggatggctc ttatttctat caaaagctag
      241 attcttagca tcttctattt tgtaagcagt ggttatgtta tagttattct ccaaaaagaa
      301 tctgacacca gagtcgtcca tatccgacca atcgtggtca tccatatcag tccatggaac
      361 tcctttcttt acaatagccc tattagcaaa ctcatcataa aatagcctac ccttaaccct
      421 tggatcattt tccaatacca ctaaagcatt ttgtatagtg ttctcaatct cacctttttt
      481 gttatagctt aatttctcca tccagtctaa atcagctact tctacttcac caaattcaaa
      541 ctcatcatct aaattttcaa atctttcttt acctaatgta atttttactt tttcatcact
      601 agatgctaaa tctatcattt ttgtaaaaga gggtaatcta tttacaggag tgttctcttt
      661 aacaccatca ctctcatcta aacctccaaa tttatgaatc ctgactaaat caaaagcatt
      721 gcatagtttt tcacttatag ggtctgtacc gtggtgtgaa tatgcgaatt tatcttcata
      781 cactacaagc ccaccaacgg tactaccttt cttataagtg tacctgcctt catgagttgt
      841 cggagcatat acatctgcta aatatttttc aattacttca gaaatagaat atgtcctaca
      901 aaaagcgccg ataaaacctt ttttctcata agggtcttct tgtcttttgg ccttattgga
      961 aattaaatta tcttctctac tacttttagg ccattcatta atatccgtcc aatcaaaata
     1021 tgaatcaagt attttatcag catctaaaag acttcccttt tggtgtttaa atacatattc
     1081 cccatcctta gctgtgctag gccagtacat taatcggact ggtgtgtaag ttgtatcatc
     1141 aaacatatca atccctatat cataagccac acgtcttgca atagcttggt actcatctgg
     1201 aagaactggc ctatttagtg ggataattaa tctgattcta ggtttatctg gt
//
LOCUS       DATGSI010000535         1178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_83828, whole genome shotgun sequence.
ACCESSION   DATGSI010000535 DATGSI010000000
VERSION     DATGSI010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83828"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /gene="flgB"
                     /locus_tag="VK031_08275"
     CDS             complement(<1..365)
                     /gene="flgB"
                     /locus_tag="VK031_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HLS53954.1"
                     /translation="MSDLAYDLIKKGLDAASLRQRTISSNIANINTPNYKVNKVEFEN
                     LLDQAIRGIGLKKTHKDHFGIGEIKDVEAIVRKRTNTFLNDNGNNVDIDLEMTELAAN
                     EIYYSTLIKQVNAKLGNLS"
     regulatory      complement(431..512)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(640..1011)
                     /locus_tag="VK031_08280"
     CDS             complement(640..1011)
                     /locus_tag="VK031_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53955.1"
                     /translation="MDTNLENLLAKRDQLIKNIEEGINGWEGSVEEAVKIVEKNQTHF
                     KNLESLDKELRAISHPSIYSKEYEDRLKDILQAQKELIGKIKAKKESLLHGMKQLKKK
                     EKVINNYISQDKRSIFIDKDL"
     gene            complement(996..>1178)
                     /gene="fliS"
                     /locus_tag="VK031_08285"
     CDS             complement(996..>1178)
                     /gene="fliS"
                     /locus_tag="VK031_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HLS53956.1"
                     /translation="IFELMNTLNFEAGGEIAQNLYNLYDYMYTGLVRANIDKDMEKTK
                     EIRKYLEELRDAWTQI"
BASE COUNT          315 a          248 c          157 g          458 t
ORIGIN      
        1 tagcttaagt ttccaagttt tgcattaacc tgcttgatta aggtagaata atagatttca
       61 ttggctgcca attccgtcat ttctaaatca atatccacat tgtttccatt atcgttaaga
      121 aaagtattgg tcctttttct cacaatggct tccacatctt taatttctcc aatgccgaaa
      181 tgatccttat gggtcttttt gaggccaatt cctcttatgg cctgatccaa taagttttca
      241 aattccacct tatttacctt gtaattaggt gtatttatgt tagctatatt gcttgatatg
      301 gttctttgcc ttaatgaggc agcatctaga cccttcttta ttaaatcata ggctaagtca
      361 ctcatttttc tccctctttc tacaataaat gcccaaatat ctctttttat acaaaaaaaa
      421 gccatcctac taaagcaact ggctgccaac ccaaagggta aggccctaca gctttgcgtc
      481 cctatttttc aataggtttg cccttgtttt attggaaagc taatacttcc tctttagaga
      541 taatattatc acacaaattt acatttatca acaaaaaaac caaaaagacg ttatactagt
      601 aacgtctgga aaaatggtat gagattggat ctaaacaaac tataaatcct tatctataaa
      661 tatggacctt ttatcctgag atatataatt atttatcacc ttttcctttt tctttaactg
      721 cttcattcca tgtaaaaggc tctctttttt agcctttatt ttccctatta attccttctg
      781 agcctgcagg atgtctttta acctatcctc atactcttta gagtaaatgg atgggtgaga
      841 gatggccctt aattccttgt ctagtgattc taaattttta aaatgtgtct gatttttttc
      901 tactatcttt acagcctctt ctactgagcc ttcccagcca tttattcctt cttctatgtt
      961 tttaataagc tgatctcttt tagctagcaa attctctaaa tttgtgtcca tgcatctctc
     1021 aactcttcca ggtacttcct tatttccttt gtcttttcca tatccttgtc tatattggcc
     1081 ctgactaaac ctgtatacat ataatcgtat aggttatata ggttctgggc tatttctcct
     1141 cctgcctcaa agtttaaggt gttcattagt tcgaatat
//
LOCUS       DATGSI010000536         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_226996, whole genome shotgun sequence.
ACCESSION   DATGSI010000536 DATGSI010000000
VERSION     DATGSI010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226996"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VK031_08290"
     CDS             <1..1239
                     /locus_tag="VK031_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HLS53957.1"
                     /translation="RQVLEEFEHNEIIYEYDDLEDEELIEVTSAPIVRLLNSIVEQAV
                     RNKASDIHVEPYSNEVRIRIRVDGDLQKIMTLPKNNLSPIITRIKIIGKMNIAEKRIP
                     QDGRVETIINGKEIDMRISTLPTVYGEKAVIRLLDKAGLSFTKESLGFNTENLKVFNE
                     ILYQPYGMILVTGPTGSGKTTTLYTALRELNTMEKNIITVEDPVEYKLDGINQVQVNA
                     KAGLTFATGLRSILRQDPDIIMIGEIRDSETAEMAIRAAITGHLVLSTLHTNDSPSAV
                     ARLVDMGVEPYLVSSAVIGIMSQRLIKVLCNNCKKAYEASYAEKKFLNYPVNQDLTLY
                     SAIGCNTCNQGYRGRVGVHEIMPIDAKMRRLIDENVNIEDLREAAIKSGMVPLTEAVM
                     NLAIEGRTSFDEVVKVGFTL"
     gene            1253..>2252
                     /locus_tag="VK031_08295"
     CDS             1253..>2252
                     /locus_tag="VK031_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HLS53958.1"
                     /translation="MKLSEIVKQGTVREASDIHITVGSPIIFRINGILVPLNDRKLNP
                     EDTKSLVFQALDDRLRAKLKEKGEIDTSYSIPGVGRYRVSAFLQRGSYSMVFRIIPLR
                     IPTMEELGLPSIVKTFSEYRRGLILVTGPTGSGKSTTLATIIDKINSERKCHILTLED
                     PIEYLHKHKKSIVNQREIGSDSKNFKAGLRAALRQDPDVILVGEMRDLETISIALTAA
                     ETGHLVLSTLHTSGAAKTIDRIIDVFPAHQQQQARVQLSSVLQAVISQQLLLSTNEDR
                     RVAAFEIMLATPAIRNLIREDKIHQIDISIQTGSNVGMITMDKSLIDLFRRGLISRE"
BASE COUNT          789 a          345 c          490 g          628 t
ORIGIN      
        1 aggcaggtat tagaagaatt tgaacataat gaaattattt atgaatatga tgatttagaa
       61 gatgaagaat taatagaggt gacatcagca cctatagtta ggctattaaa ttcaatagtt
      121 gaacaggctg taagaaacaa agcatctgat atacatgtgg agccttattc caatgaagta
      181 aggataagga taagagtaga tggagacttg caaaaaatta tgaccctacc taaaaacaac
      241 ttatctccaa taataaccag aataaaaatt ataggaaaga tgaatatagc agaaaaaaga
      301 atcccccagg atggacgggt ggaaaccatc ataaatggca aggagataga catgagaata
      361 tcaaccttgc ctactgtata tggggagaag gctgtaataa ggttacttga caaggcagga
      421 cttagtttta ctaaggagag cctagggttt aatacagaaa atttaaaagt ttttaatgaa
      481 attttgtatc aaccctatgg catgatttta gttacagggc ctacaggtag tggtaagacc
      541 accaccctat atactgccct aagagaacta aacactatgg agaaaaatat tattaccgtt
      601 gaagatccgg tagaatataa acttgatggt ataaatcagg ttcaggtaaa tgctaaggca
      661 ggactaactt ttgccacagg tctaaggtca atcttaagac aagatcctga tataattatg
      721 ataggggaga ttcgagacag cgagacggct gaaatggcta ttagggctgc tataactgga
      781 catttagtcc tgtctacttt gcataccaat gacagcccat cggctgtggc cagattggtt
      841 gatatgggag ttgagcccta tttagtatct tctgctgtca ttggcattat gtctcaaagg
      901 cttataaagg ttttatgtaa taattgtaaa aaggcctatg aggccagcta tgctgagaag
      961 aaatttttaa attatcctgt taatcaggat ctaacccttt attcagctat aggatgtaat
     1021 acctgtaacc aaggatatag aggtagggtt ggagtccatg aaattatgcc tattgatgca
     1081 aaaatgagaa ggttaattga tgaaaatgtt aacattgaag acctaagaga ggctgccata
     1141 aaatctggta tggtgccttt aacagaagct gtaatgaacc tggctatcga aggaagaaca
     1201 tcctttgatg aagttgtaaa ggttggattt accttataat aggaggcaga aaatgaaatt
     1261 atctgaaata gttaagcaag gaacagtcag ggaagcatct gacatccata taactgtagg
     1321 ttcgcctatt atatttagaa taaatgggat cctagttcct ttaaatgata gaaaactgaa
     1381 tccagaggat acaaagtctc tagtctttca agccttggat gataggttaa gggctaagct
     1441 taaagaaaaa ggtgaaatag atacttccta ttccattccg ggagtaggta gatacagggt
     1501 aagtgccttt ttacaaaggg gtagctatag tatggtattt aggataattc ctttaagaat
     1561 accaaccatg gaggaattag gcctacctag cattgtaaag actttttcag agtatagaag
     1621 aggtttgata ctggttactg ggcccactgg cagtggaaaa tctaccacct tagccaccat
     1681 tatagataaa atcaatagtg aaagaaaatg ccatatactt actttggaag atcctataga
     1741 atacttgcat aaacataaaa agtctatagt aaaccaaaga gaaataggta gcgattcaaa
     1801 gaatttcaag gcaggattaa gggctgcttt aaggcaagat cctgatgtaa ttttggtagg
     1861 tgagatgcgt gatttagaga ctatctcaat agcccttact gcagcggaaa ctgggcattt
     1921 ggttttatca accttgcata cttcaggtgc agctaaaacc atagatagaa taattgatgt
     1981 atttcctgcc caccagcagc agcaagccag ggttcaacta tcttctgtct tgcaggcagt
     2041 tatttctcaa cagctccttt taagtactaa tgaagataga agggtggctg cctttgagat
     2101 aatgctggct acaccagcca ttagaaattt aattagggag gataagattc atcaaataga
     2161 tatatctatt caaacaggtt ccaatgtagg gatgataacc atggataagt ccttaataga
     2221 tttatttagg agaggattaa ttagtagaga aa
//
LOCUS       DATGSI010000537         1129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_34645, whole genome shotgun sequence.
ACCESSION   DATGSI010000537 DATGSI010000000
VERSION     DATGSI010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34645"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..122
                     /locus_tag="VK031_08300"
     CDS             18..122
                     /locus_tag="VK031_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HLS53959.1"
                     /translation="MDRKLKRASHKNIIHKNAVARQISKLTKKLNEAM"
     gene            complement(227..>1129)
                     /gene="holA"
                     /locus_tag="VK031_08305"
     CDS             complement(227..>1129)
                     /gene="holA"
                     /locus_tag="VK031_08305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HLS53960.1"
                     /translation="SLESLNFSRIQGKDGTLDRLINACETLPFMASKKIVLVRDISAF
                     LKALSPKEEKDFYQYMDSLGEHICLILMDDNSSVKKNYKIYKHFNKKNQVVEFGKLTD
                     RDLNQWVLSLLRKHGKTMLASDINYFLQLSSYRSRNITVSLFELENALLKLINYVKDK
                     EITREDIDIVLIKSIDTNIFELLDAINQSDAEKALSIFNEMYMTNEPILGMLGMITRQ
                     VRLMLSYQLYRQKGYGDSSIQAKLGIGNYEYRIIKSRSRKFTVNQLENIMKELLNVDI
                     KIKTTAVDDKLLMEMLLVKMCNNK"
BASE COUNT          384 a          211 c          136 g          398 t
ORIGIN      
        1 aagaattaat aaaagtaatc gatagaaaac taaagagagc aagccataaa aatataattc
       61 ataaaaatgc tgttgcaaga caaataagta aattaactaa aaaattaaat gaagcaatgt
      121 agatctatat aataggtctt acaaaacaga cggaaagttc ttttcttagc aaggtcgaac
      181 tttcctatta tttttttgca ataaaaaact tcggtttccc gaagttttat ttattattac
      241 acattttcac caaaagcatt tccataagta acttatcatc aactgcagta gtcttaatct
      301 taatatctac atttagtaat tccttcatta tattttccaa ctgattcaca gtaaactttc
      361 tgcttctaga cttaataatc ctatattcat aatttcctat gcccaattta gcctggatac
      421 tgctatcacc atagcccttt tgcctatata attgatagct tagcattagc ctaacctgtc
      481 gcgtaatcat acctaacata cccaatatag gctcattggt catatacatc tcattaaaaa
      541 tagatagggc tttttcagca tctgactgat ttatggcatc taaaagttca aatatattgg
      601 tatcaattga ttttattagt acaatatcta tatcctccct agttatttct ttatccttta
      661 cataattaat aagctttagc agggcatttt caagttcaaa caagctaaca gtaatatttc
      721 tactcctata ggaactaagc tgtaaaaaat aatttatatc agaggctagc atggtctttc
      781 catgttttct taaaagactt aaaacccatt ggtttaaatc cctatctgtt aacttaccaa
      841 actcaaccac ctgattcttt ttgttaaaat gtttgtagat tttataattt ttcttcacag
      901 aggaattatc atccatcaat ataaggcaaa tatgttctcc taaactgtcc atatactgat
      961 aaaaatcctt ctcttccttt ggacttaagg cttttagaaa tgccgaaata tctcttacca
     1021 atactatctt tttactagcc ataaagggta gggtttcaca ggcatttata agcctatcta
     1081 aggtcccatc ttttccctgt atcctagaga aatttaagct ttccaagga
//
LOCUS       DATGSI010000538         1304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_34770, whole genome shotgun sequence.
ACCESSION   DATGSI010000538 DATGSI010000000
VERSION     DATGSI010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34770"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1066)
                     /locus_tag="VK031_08310"
     CDS             complement(95..1066)
                     /locus_tag="VK031_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005955683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HLS53961.1"
                     /translation="MEEKVVDQGKETVALDGALTASLAPHIKSNETVRSVMLDVIIAL
                     TPAMIGSIYFFKLSALKLILVSVISAVAFEAATQKFFKKDILIGDLSAVVTGILLAFN
                     LPANAPWWIAVFGAGFAIIIVKEFFGGIGSNFMNPALAARAAIVASWPSIMADYVNPD
                     GITSATPLSIIKDGGGVSALPSIKRMLIGDIGGSLGETVTILLLIGGIYLLIRQVIEW
                     RIPTFYILSTVAMLLVLGVEPELITYHVLGGGLILGAFFMATDYSSSPVTPKGKIVFA
                     IGAGILTAIIRVKGSMPEGVSYAILLMNVATPVIERFTKPKVFGEVK"
     gene            complement(1082..>1304)
                     /locus_tag="VK031_08315"
     CDS             complement(1082..>1304)
                     /locus_tag="VK031_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLS53962.1"
                     /translation="QCGKCIEVCPINLEPLFISAYALKDRFEEAEALSAEACIECGSC
                     SYICPSKRPLSESIAFAKQEIRANRRKSQ"
BASE COUNT          391 a          273 c          211 g          429 t
ORIGIN      
        1 taggtgctgt aaattcattg gatatggcca gtacacctgc tgatattgct gtaaacaata
       61 ataatactat tcctaattta attgtttccc tcatctattt tacctcccca aacacttttg
      121 gctttgtgaa tctttcaatt actggagtgg caacattcat taatagaatt gcataggaaa
      181 ctccttctgg catactgccc ttaactctaa ttattgcagt taaaatacca gcaccaatgg
      241 cgaaaactat tttaccctta ggtgttactg gtgaagatga atagtctgtt gccataaaga
      301 aggctcctag tataagacct ccacctagaa catgatatgt aatcagctct ggctctactc
      361 ctaaaactag taacatggct actgtactta gtatataaaa ggttggtatt ctccactcaa
      421 ttacctgtct aattaatagg taaattccac ctattagtaa aaggattgta actgtttctc
      481 ctaaggagcc acctatatct ccaattaaca ttctttttat tgatggtaag gctgatactc
      541 cgccgccatc ttttattatt gataaaggtg tggcactagt tattccgtca ggatttacat
      601 aatcagccat tatagatggc caggatgcca ctatggctgc tctagctgct aaggctgggt
      661 tcataaagtt tgaacctata ccaccgaaaa attccttaac tattataatt gcaaatccag
      721 caccaaatac tgcaatccac caaggtgcat tggctggcag gttaaaagct agtaaaattc
      781 cagttactac tgcacttaag tctccaatta aaatgtcttt tttgaaaaac ttctgagttg
      841 ccgcttcaaa ggctactgca gaaattactg atactaggat cagttttaaa gcagatagtt
      901 taaagaaata tatactacca atcatggctg gagtcagtgc tattattaca tctaacatta
      961 cacttctaac agtttcattg gacttaatat gaggtgccaa ggatgctgtt agtgcaccgt
     1021 ctaaagcaac tgtttctttg ccctgatcta caactttttc ttccattata cttcctccta
     1081 actattgtga ttttcttcta ttggctcgta tttcctgttt tgcaaaggca attgattcgc
     1141 ttaatggcct tttagatgga caaatataag aacaggaacc acactctata caagcttctg
     1201 cacttaaggc ttctgcttcc tcaaacctat cctttagcgc ataggcacta ataaataaag
     1261 gttctaagtt tataggacat acttctatac atttaccaca ttgg
//
LOCUS       DATGSI010000539         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_415346, whole genome shotgun sequence.
ACCESSION   DATGSI010000539 DATGSI010000000
VERSION     DATGSI010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415346"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VK031_08320"
     CDS             complement(<1..730)
                     /locus_tag="VK031_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HLS53963.1"
                     /translation="MTKKQSLGRGLGALIPQMIPEYSLEDKEVNEPSKLAEEGILKLP
                     IDRIEAREDQPRKKFDEDSLNELKESIIEYGIIQPIVVRKISDNYQIIAGERRFRAAK
                     LAELEEIPVRVMEMDDQSIKEVALIENIQREDLDPIEEALGYAQLMEDYSYTQKEVSE
                     RLGKSRSYIANAIRLLGLDPEIKDALRDKLITPSQGRTLLSVKNKEERLKYLEQFKAK
                     TLNIRDVEDAVRNSNDTAKTKKSSK"
     gene            complement(755..>1526)
                     /locus_tag="VK031_08325"
     CDS             complement(755..>1526)
                     /locus_tag="VK031_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HLS53964.1"
                     /translation="EMVNIENRESILKKSLREVDGYDYILIDCPPSLGVLSINALTAS
                     DTVLIPIQCEYYALEGVSQLISTIDLVKMALNPKLEVEGILMCMFDGRNNLSLEVVEE
                     VKKYFKNQVFKSVIPRNIKLAEAPSHGTSILKYDDESKGAIAYKRLSLEIIEKNKVKK
                     TTEKPEDNSEADKKNIDLKDENSKEQSNKVESEKEIEENLEIEEEITEEIKEENSDII
                     EVDTLLEGEELVDVKEIDLNTKSTEEEIIKEDEEAKNI"
BASE COUNT          423 a          301 c          159 g          643 t
ORIGIN      
        1 ttttactact ttttttagtt ttagctgtat catttgaatt tcttactgca tcttctacat
       61 ctcttatatt taaagtttta gctttaaatt gttctaaata ttttagtctc tcttctttat
      121 tttttacaga aagtaaagtt ctaccttgag aaggagttat aagtttatct cttaaagcat
      181 ccttaatctc tgggtctaaa cctagaagcc tgatagcatt tgctatataa gatctacttt
      241 tacccaatct ctcacttacc tctttttgag tataggaata gtcctccata agctgagcat
      301 atcctaaagc ctcttctata ggatctaagt cctccctttg gatattttca atcaatgcga
      361 cctcttttat agactggtca tccatttcca ttacccttac aggaatctct tctaattccg
      421 ccaatttagc agctctaaat cgtctttcac ctgcaattat ttggtaatta tcactaattt
      481 ttctaacaac tatcggctgg ataattccat actctatgat agattccttt aattcgttta
      541 aactatcctc gtcaaatttt ttacgaggtt gatcttctct tgcttctatc ctatcaatag
      601 gcagttttaa tataccttct tcagctaatt tagaaggttc atttacttcc ttgtcttcca
      661 aagaatattc aggaatcatc tgaggaatta aagcacctaa acctcttcca agactttgtt
      721 ttttagtcat taaatcatca cacctatctt aatattatat gtttttagcc tcttcatctt
      781 ccttgataat ctcttcttca gtagattttg tatttaaatc tatttctttt acatctacta
      841 attcttcacc ttctaaaaga gtgtccactt ctattatatc ggaattttct tcttttattt
      901 cttctgtaat ctcttcctca atttctaagt tttcctctat ctctttttct gattctactt
      961 tattagattg ttccttagaa ttttcatctt ttaaatctat atttttttta tcggcttcag
     1021 agttatcttc aggcttttca gttgtttttt taactttatt tttttcgata atttctagac
     1081 ttaatctctt ataggcaata gcacccttag attcatcatc gtatttaagt atacttgtac
     1141 catgactagg agcttcagct aattttatat ttctaggtat cactgattta aagacttgat
     1201 ttttaaagta tttctttact tcttctacaa cttctaaaga cagattattt cttccgtcaa
     1261 acatacacat caatatacct tcaacttcta atttaggatt taaagccatc ttaactaaat
     1321 ctatagtaga tattaattga ctaacacctt ctaaagcata atactcacat tgtattggta
     1381 taagtaccgt atcagaagct gtaagagcat ttatacttaa tactcctaat gatggaggac
     1441 aatctattaa gatataatca taaccatcta cttcccttaa agattttttc aatatacttt
     1501 ctctattttc aatatttacc atttca
//
LOCUS       DATGSI010000540         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_652168, whole genome shotgun sequence.
ACCESSION   DATGSI010000540 DATGSI010000000
VERSION     DATGSI010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652168"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..453)
                     /locus_tag="VK031_08330"
     CDS             complement(19..453)
                     /locus_tag="VK031_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53965.1"
                     /translation="MNIGDDVHKVTSISNKRNNRALVRIDEDRLGRLFDNPLRSPNEE
                     DVYYLETGGGNLEVFGLVDTLVVRFLKVPKKAYLSMKTVTITAGSRTVERTEPDPDNS
                     VNLEWSNTEYKTILNRVLNMFSTPNRDIFVTQKTERDKVSNE"
     gene            complement(849..>1113)
                     /locus_tag="VK031_08335"
     CDS             complement(849..>1113)
                     /locus_tag="VK031_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53966.1"
                     /translation="LNKVEHQPEVVNRNFATAETKLKSKIIGLFRNGVLKNTNGIVTW
                     ADGGLEVGQFKVTEENKLVDQMVAFVAKGSKEAKAFEKKLAEI"
BASE COUNT          269 a          238 c          166 g          440 t
ORIGIN      
        1 atggttgtgt tattactgtt actcattgct tactttgtcc ctctctgtct tttgggttac
       61 aaatatatct ctattcggag tggagaacat attaagtacc ctgttaagaa tcgttttata
      121 ctctgtgttt gaccactcta agttgactga attgtctggg tctggctctg ttctttctac
      181 tgttctacta cctgccgtaa tagttaccgt tttcatggaa aggtatgcct ttttaggtac
      241 tttcaggaat cttacgacca atgtatccac aagaccaaaa acctccaaat taccacctcc
      301 tgtttcaagg taatagacgt cctcttcatt tgggcttcta agcggattat caaacaatct
      361 tcctaatctg tcttcatcta ttctgactaa tgctctgttg tttcttttat tactaataga
      421 agttacctta tgtacgtcat ctcctatatt cacctcagaa tctattacga ctacctcttt
      481 tttatctcta aatgggtcta gtctgtggtc tgcgtcttga ttgttaccat aacctactct
      541 tgtccttccg tctactcttt tattagtggc ccctattaat tctttgaagt actccccctg
      601 tactgcattt actattctgt taaactcttc tacctaaata aaccttccat tgttcttgga
      661 tagttgttca ttcactgagt tatataaatc tataatatta atcatctctc ttctttatct
      721 ttctttcttt tgtacaaata taaagaactt taatagtggt ttatggtaag ggtcttttta
      781 aaaatgaaaa acccctccga agaggggcag gttaaacaga tggttattag ataaccttta
      841 tagttgtctt atatttcagc tagtttcttc tcgaatgctt tggcttcttt agagccttta
      901 gctacgaagg ctaccatctg gtctactaac ttattttctt cagtcacttt aaactgtcct
      961 acttctaatc ctccatctgc ccatgtaacg attccgttag tgttctttaa aactccgttt
     1021 cggaatagtc caataatctt agatttaagc ttagtctctg ccgtagcaaa gtttctgttt
     1081 accacttctg gttggtgttc aactttatta agt
//
LOCUS       DATGSI010000541         1305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_84316, whole genome shotgun sequence.
ACCESSION   DATGSI010000541 DATGSI010000000
VERSION     DATGSI010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84316"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VK031_08340"
     CDS             <1..524
                     /locus_tag="VK031_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HLS53967.1"
                     /translation="FLITDTVGFVSKLPTHLIEAFKGTLEEVRYADLILHVVDASNED
                     LDIQISTTLDILNSLEVLDKPIITVFNKMDLVELKELVYDNYSIDEKIFISAKDTSDL
                     NRLLELIEEKLPQKFKKVWIQIPYDKQQLLNYLMNNYEVEDISHQVEGSLVNLSINSM
                     DLERFKEFVIDRS"
     gene            553..993
                     /locus_tag="VK031_08345"
     CDS             553..993
                     /locus_tag="VK031_08345"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HLS53968.1"
                     /translation="MIIRNCAGGVVFSGKKVLILKNEKNEWVLPKGKIRNNNLPPETA
                     IARVEFETGVKGKIVSSAGETAYEFYSITRNQPVCNKIVWYIMEAEDENYKINSEEGF
                     QDGGFYPIEEAIEMITYSQDKSLVSLSYNRYLEFKEYEKRMANL"
     gene            1031..>1305
                     /locus_tag="VK031_08350"
     CDS             1031..>1305
                     /locus_tag="VK031_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HLS53969.1"
                     /translation="MKENYRTTFSYGSDEIIINKSRFIGYSMPINNEEEAIEFIEKIK
                     AKHRDATHNVYAYVVGPNSNIQRFSDDGEPSGTAGIPALEVIKKEDL"
BASE COUNT          484 a          168 c          269 g          384 t
ORIGIN      
        1 atttcttaat aacggatact gtaggttttg tatcaaaact tcctactcat ttaattgagg
       61 cctttaaggg gaccttggag gaagtaagat atgcagactt aatcttacat gtggtagatg
      121 catccaatga ggacttagat atacaaatat ctaccacctt agatattctg aatagcttgg
      181 aagttttaga caagccaatt ataaccgttt ttaataaaat ggatttggta gaacttaaag
      241 agcttgtata tgacaattat tctatagatg aaaagatttt tatatcagca aaggatacat
      301 cagatttaaa taggctgcta gaattaattg aagaaaagct tcctcaaaaa tttaaaaaag
      361 tatggattca aattccctat gacaaacaac aacttttgaa ctatttgatg aataattatg
      421 aggtggagga tattagccac caagtggagg ggtccctggt taatttatca ataaattcta
      481 tggatctgga aaggttcaaa gagtttgtaa tagataggtc ttaaatatat aaatgatagg
      541 gagatgatac aaatgataat aaggaattgt gctggagggg tagtattcag tggcaagaag
      601 gttttaattt taaaaaatga gaagaatgaa tgggtacttc ctaaagggaa aataagaaac
      661 aataacctgc ctcctgaaac tgccattgcc agggtagagt ttgaaactgg agtaaagggc
      721 aagatagttt cctctgcagg ggaaacagcc tatgaatttt actctatcac aagaaatcag
      781 ccggtttgta ataagatagt ttggtatatc atggaagctg aggatgaaaa ttacaaaata
      841 aattctgagg aaggctttca agatggaggc ttttatccta tagaggaggc tatagagatg
      901 ataacttata gccaggataa atccttagtt tctttatcat ataacagata tttagagttt
      961 aaggaatatg aaaaaagaat ggctaattta taatataagc aatctcactt tgaaagggag
     1021 ataaaagtag atgaaggaaa attacagaac aactttttcg tacggtagcg atgaaattat
     1081 aattaacaaa tcaagattta ttggttattc catgcctatt aataatgaag aagaggcaat
     1141 agaatttata gaaaagatca aggccaaaca tcgggatgcc acccataatg tttatgccta
     1201 tgtggttggt cccaattcaa acattcaaag gtttagcgat gatggtgaac cttcaggaac
     1261 agctggtata ccagctttgg aggttattaa aaaagaagac ttaag
//
LOCUS       DATGSI010000542         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_34939, whole genome shotgun sequence.
ACCESSION   DATGSI010000542 DATGSI010000000
VERSION     DATGSI010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34939"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            588..2489
                     /locus_tag="VK031_08355"
     CDS             588..2489
                     /locus_tag="VK031_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLS53970.1"
                     /translation="MMKRNLVLLLILSLVLSTFLIGCTPAEQDTGQQQDDGQEEEPAG
                     APEEPVGEIKIGDTTELSGDWVPYWTNNAADYNVYNFITGYGTVDMKFDGEYVVNETV
                     VKDYQVEENEDGSKTYIWTINDNLVYADGTPITAEDYVASALLWSSKQVQDTKGKASY
                     GFYWKGYDAFNTGEKKEFEGVRLLDEYTFSVTIDESNLPYFYELPMVSVAPTQLAYWT
                     DETVEIKDDGEGAYFSDNFTAEAYKERFEKARFGVEDFPASGPYKLVEYDESAKTAVM
                     EVNENYLGNWEGQKPKIKTIIYKKVTNETALNELETGGVDILRGLSSGDEIDGGLDLV
                     EKGGFDFADYPRAGYGQLQFVCDFGPTKDAEVRQAIAHLLDRNDFATAFTGGHGTVVH
                     GPYGEAQWFYQETKADINAKVNTYPYDPEKAKELLEQAGWTLDKDGNEYKEGLRHKKD
                     EDGNIVPLVIEWASSEDNPVSDLLVTKLMENPDVAAVGMEIKKTEMSFDELLNYIYRE
                     GAEDPKYGVPTYHMFNLGTGFNPTYDMTTTYTDDPAMIEAGYNTNFIADEELAKFARE
                     MVLVDPEDREGFKENFVNFIVRWNELLPDLPLYSNIYHDFFTDKLKNYETNDIVKISD
                     SLLYAYVEE"
     gene            2646..3632
                     /locus_tag="VK031_08360"
     CDS             2646..3632
                     /locus_tag="VK031_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLS53971.1"
                     /translation="MTRYILKRIMYMIFVFAIMSVILFSLYEMIPGDPARAEVEHLKT
                     QLRPEEYERAYQQARKDWGLDDPIHEKYIRWAKGLLKGDLGYSHVHKKEVNALIKTPL
                     KNTLFINFFSVTAGLLITIPLGVYCAINQHSKFDKVVQVLTIIGHSIPSFIFGLLFIF
                     VFSVKLRWFPVSGMSTPNLKGSDWVLFKDKVWHLALPLMVMTISTLAGLTRYIRAAMI
                     ESLSMDYIKTARAKGLKEKVVIWSHAWRNALLPIITLLVSWFASIFYGSLIIESMFNL
                     NGLGKFLIDALNNKDFNLVMAVQLLYIVVALLSNLITDISYGLVDPRVRVDR"
     gene            3650..>3865
                     /locus_tag="VK031_08365"
     CDS             3650..>3865
                     /locus_tag="VK031_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53972.1"
                     /translation="MDGNKVTEKNKETKERTSFSRLLFRNLFGGRRELSIFEEEEVQG
                     PWRTVFATFLSNKMAMTGLVIFLLIFLT"
BASE COUNT         1388 a          575 c          750 g         1152 t
ORIGIN      
        1 aagaattaaa agaaaagtaa tagaagctta tcagattcct ttttatgaca aaaataacat
       61 atttttttct cacaacatgt gacaaatagc tagttttaca tctttgtaag gaaaaattgg
      121 ctggcttttt ctatatttgc tttattttta aaggagccat gaaaaaataa actaatgttt
      181 ccttgcatta ctggttaaat gttgctataa ttataataat ttattcaaag atagaattta
      241 aacaagcctt gctaaggttt ttttattata attttcactt ataataatca tacgaacatt
      301 gataataggg taaattacac atctgtaaga ctaataataa atagcagttg atatttttta
      361 agaaataata tagaatgtaa tagacattac agctttagca tgataaaatc ctatcacgtt
      421 acaagacttt agctataaaa attcataaat tatacttgtg attaatatgt taaataaagg
      481 ttgactaatg taatataaaa gccttattgt aggtaagcac aagttaaatc taaaaaagta
      541 gtagctttat aactactttg aaatataatt taagggggaa agcttagatg atgaaaagaa
      601 acttagtact attactaatt ctatccttgg ttctttcaac attcctaata ggctgtacgc
      661 cagctgaaca agacacaggc caacagcaag acgatggaca agaggaagaa ccagcaggag
      721 caccagaaga gcctgtagga gaaataaaaa taggtgatac tacagagctt tcaggtgact
      781 gggttccata ttggaccaac aatgctgctg actataatgt gtataatttt ataacaggtt
      841 atggtacagt agatatgaag tttgacggtg agtatgtagt taacgaaact gtagttaagg
      901 attaccaagt agaagaaaat gaagatggat ctaaaactta tatttggact atcaacgaca
      961 atttggttta tgctgatggt actccaataa ctgcagaaga ttatgttgct tctgccctac
     1021 tatggtcttc aaagcaagtt caagatacta agggtaaggc aagctatgga ttctactgga
     1081 aaggctatga tgcctttaac actggagaaa agaaagagtt tgaaggtgta agattactag
     1141 atgaatatac attctcagta acaatcgacg aatctaacct accatacttc tatgaattac
     1201 caatggtttc agtagctcca acccaattag cttactggac agatgaaact gtagaaatca
     1261 aagatgatgg tgaaggtgct tacttctcag acaactttac tgcagaagct tataaagaaa
     1321 gatttgaaaa ggctagattt ggtgtagaag acttcccagc ctcaggacca tacaaattag
     1381 ttgaatatga tgaatcagct aaaacagctg taatggaagt taacgaaaac tacctaggaa
     1441 actgggaagg ccaaaagcca aagataaaga ctataatcta taagaaggta actaacgaaa
     1501 ctgcacttaa tgagttagaa acaggcggag ttgacatctt aagaggattg tccagtggag
     1561 acgaaatcga tggtggctta gatctagttg aaaaaggtgg tttcgatttt gcagattatc
     1621 cacgtgctgg atatggacaa ttacaatttg tatgtgactt tggacctact aaagatgcag
     1681 aagtacgtca agctatagct cacttattag atagaaatga ctttgctact gcctttacag
     1741 gtggacatgg tacagtagta catggaccat acggtgaagc acaatggttc taccaagaaa
     1801 ctaaggctga tataaatgct aaagttaaca cttacccata tgaccctgaa aaagcaaaag
     1861 aacttctaga gcaagctggt tggaccttag ataaggatgg taacgaatat aaagaaggtc
     1921 taagacataa gaaagacgaa gatggaaaca tagttccact agtaatcgag tgggcttctt
     1981 cagaagataa cccagtatca gacctattag ttaccaagct aatggaaaac ccagatgtag
     2041 ctgctgtagg tatggaaatt aagaaaactg agatgagctt tgatgagcta ttaaactata
     2101 tctatcgtga aggagcagag gatccaaaat atggagttcc aacatatcat atgtttaacc
     2161 taggtactgg atttaaccca acttatgata tgactaccac ttatactgat gatcctgcca
     2221 tgatagaagc tggatacaac accaacttca tagcagacga agaattagct aagtttgcta
     2281 gagaaatggt acttgtagat ccggaggata gagaaggatt taaggaaaac tttgtaaact
     2341 ttattgtaag atggaacgaa ctattaccag acctaccatt atactccaat atctatcatg
     2401 acttcttcac tgataagttg aagaactatg aaactaatga tatagtaaaa atcagtgact
     2461 ctttactata cgcttatgta gaagaataat ttttaaacac aaatatattt gatacaaaat
     2521 agggtatgca aataccctat ttttacataa gataggcttc cccaaattta aatttgggga
     2581 agtctattga acattaattt agtaggacta tatttaaata aatttagaca aggtaggtat
     2641 ataaaatgac tagatacatt ttaaagagaa ttatgtacat gatatttgta tttgcaataa
     2701 tgtcagttat cttattctcc ttatatgaaa tgataccagg tgaccctgcc agagctgagg
     2761 tagaacatct taaaacacaa ttaagaccag aggagtatga aagggcctac caacaagcta
     2821 ggaaagattg gggactggat gatcctatcc atgagaaata tataagatgg gcaaaaggat
     2881 tattaaaagg agatttggga tattcccacg tacacaaaaa ggaagttaat gctttaatta
     2941 agactccctt gaagaacacc ctatttataa atttcttttc agttacagca ggtttattga
     3001 taaccatacc cctaggggtt tattgtgcaa taaaccagca ttccaaattt gacaaggttg
     3061 tacaagtttt aaccattata gggcatagta ttccctcctt tatctttggg ttattgttta
     3121 tttttgtttt ttcagtgaaa ttaagatggt tccctgtaag tggtatgagt acaccaaatt
     3181 taaagggatc tgattgggta ttgtttaagg acaaggtatg gcacttagcc ctacccttaa
     3241 tggtaatgac tatatcaact ttggctggtt taaccaggta tattagagca gctatgattg
     3301 aatccttatc aatggattat ataaagacag ccagggcaaa ggggcttaag gaaaaggtgg
     3361 taatctggtc ccatgcttgg agaaatgcct tacttccaat aataacttta ttggtaagct
     3421 ggtttgccag tattttctat ggatccttaa tcatagaatc tatgtttaac ctaaatggct
     3481 taggtaaatt tttaatagat gctttaaata ataaggactt taatctggta atggctgtac
     3541 aactactata tatagtagtt gccttactaa gcaacttgat tacagatata agttatggac
     3601 tagttgaccc aagggtcaga gtagacagat agaaggaggg taattttaaa tggatggaaa
     3661 taaagtaaca gaaaaaaata aggaaacaaa agaaagaacc agcttttccc gccttctatt
     3721 taggaacttg tttggtggga ggcgtgaact ttccatattt gaggaagaag aggttcaagg
     3781 cccatggagg actgttttcg ctacattttt gtcaaataaa atggcaatga ctggcttagt
     3841 aatcttttta ttaatatttt taact
//
LOCUS       DATGSI010000543         1833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_509184, whole genome shotgun sequence.
ACCESSION   DATGSI010000543 DATGSI010000000
VERSION     DATGSI010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509184"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VK031_08370"
     CDS             <1..190
                     /locus_tag="VK031_08370"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HLS53973.1"
                     /translation="VGGIKEKVLAANRMGIKKVLLPHENKKDLEEIPDRIKRKLEFVL
                     VKNMEDVLEHALAKGTEE"
     gene            190..774
                     /gene="yihA"
                     /locus_tag="VK031_08375"
     CDS             190..774
                     /gene="yihA"
                     /locus_tag="VK031_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HLS53974.1"
                     /translation="MRIIKADLQRVAVKPEQYPEDRLPEIAFAGRSNVGKSSLINTLV
                     NRRGLARTSGKPGKTRTINFYIVNDEFRLVDLPGYGYARVSKKEQEKWGHIINEYLSI
                     RENLVEVILIVDIRHQPTQQDVMMYEWIKSFGYSGIVIATKADKISRGKRHRHENMVR
                     QKLGIKDPQLVIPFSATDRLNKDKILKAIDHLLR"
     gene            complement(817..975)
                     /locus_tag="VK031_08380"
     CDS             complement(817..975)
                     /locus_tag="VK031_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HLS53975.1"
                     /translation="MDRWVCEPCGWVYDPAEGDPDGGIEPGVPFEELPEDWVCPMCGA
                     PKEDFYKE"
     gene            1165..1521
                     /locus_tag="VK031_08385"
     CDS             1165..1521
                     /locus_tag="VK031_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLS53976.1"
                     /translation="MKSFVDNILEKLNNILDNKDLIFDIRLIINELIINGVFHGNECV
                     DTKCVNVNLDVNGDQVTIIVEDEGEGFDFNIDSYNPLELKCGGRGLVLVKGLSDELII
                     DKNKIKAIKYLYKEDN"
     gene            1664..>1833
                     /locus_tag="VK031_08390"
     CDS             1664..>1833
                     /locus_tag="VK031_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLS53977.1"
                     /translation="MDLIKENIRNIAIIAHVDHGKTTLVDSLLQQAGIFRANQLIEDR
                     ILDSGDIERERG"
BASE COUNT          690 a          244 c          378 g          521 t
ORIGIN      
        1 tgtaggcggc attaaggaga aggtgctggc agccaatagg atgggaatca aaaaagtctt
       61 actgccacat gaaaataaga aggatctgga ggaaatacca gacaggataa agagaaagct
      121 ggagtttgtc cttgtaaaaa atatggaaga cgtattggag catgctctag caaaagggac
      181 tgaagagtaa tgaggataat aaaagcagat ttacaaagag ttgctgtaaa accagaacaa
      241 tatccagagg ataggctacc tgaaatagcc tttgctggta gatccaatgt aggtaagtca
      301 tcgctgataa acactttagt aaatagaagg ggcttggcca gaaccagtgg taaaccaggt
      361 aaaaccagaa ccataaactt ttatatagta aatgatgagt ttaggctagt ggatttgcca
      421 gggtatgggt atgctagggt ttcaaaaaaa gaacaggaaa agtggggcca tatcattaat
      481 gaatatttat caattaggga aaacctagta gaagtaatcc taattgtaga tataaggcac
      541 cagccaaccc aacaagatgt gatgatgtat gaatggataa aaagttttgg ctacagtggt
      601 atagtaatag ctaccaaggc tgataaaata tctaggggta agcggcatag acatgaaaat
      661 atggtaagac aaaagctagg aataaaagat cctcagctgg taattccatt ttctgcaaca
      721 gacagattaa ataaggataa aatattgaag gcaatagatc atctattaag atagacaaaa
      781 gaccagctga acaagtcagc tggtctttta tgtattttat tccttataga agtcttcctt
      841 aggtgcccca cacattggac atacccaatc ttcaggaagt tcttcaaaag gtacacctgg
      901 ttctatacct ccatctggat ctccttcagc tggatcatat acccaaccac atggttcaca
      961 tacccatcta tccatgtcaa ttctcctttc tctttggtta ctctttctta atttacccaa
     1021 tatttatata agtaaacatt gggtaaataa taattataag ttaaatatac cttattttgg
     1081 gtaaaatatc aatgaaaata ttttttgggc gtgataaaat tgaaattcac ctatagtgga
     1141 tctgtatgta gtgatattaa cagagtgaaa agcttcgtag ataatatatt agaaaaatta
     1201 aataatattt tagataataa ggatcttata tttgatataa ggttaataat taatgaatta
     1261 ataataaatg gagtttttca tgggaatgaa tgtgtagata ctaaatgtgt aaatgttaac
     1321 ttagatgtta atggagatca ggtaacaata atagtggaag atgagggtga aggatttgac
     1381 tttaatatag atagttataa tcctcttgaa ctgaagtgtg gaggaagggg acttgtattg
     1441 gtaaagggcc tatctgatga acttattatt gacaaaaata aaattaaggc cataaaatac
     1501 ttgtacaagg aggataatta aggaaaatat gataaaatcc taagaggctg gtataataaa
     1561 gaaagcttat accagctatt tctttgcaat taggcttgtt cacttaaact attagatatg
     1621 atagaataat tatgaaaatt tacaagatga aaggatatta tatatggatt taattaaaga
     1681 aaatattaga aatatagcca taatagccca tgtggatcat gggaaaacta ctttagtaga
     1741 tagcctgctg cagcaagcag gaattttccg ggcaaatcaa ctaatagaag accgtatctt
     1801 agattcagga gatatagaaa gggaaagggg aat
//
LOCUS       DATGSI010000544         3744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_134804, whole genome shotgun sequence.
ACCESSION   DATGSI010000544 DATGSI010000000
VERSION     DATGSI010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134804"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VK031_08395"
     CDS             complement(<1..502)
                     /locus_tag="VK031_08395"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLS53978.1"
                     /translation="MLEVTSPQNRIIKEIKSLYRRKNRWDKNLFIIEGPKIIEEALTE
                     QIKLKYLVFGESFLENKESAYLIEMAGNYQKIKVTDRLFEEISDTESPQGILAVATFI
                     ERRLEDICIKDQPFLVYLDQVQDPGNIGTIIRTADAFNIDGIILGKGTVDPYNPKVVR
                     ATMGSIF"
     gene            complement(637..987)
                     /gene="rplT"
                     /locus_tag="VK031_08400"
     CDS             complement(637..987)
                     /gene="rplT"
                     /locus_tag="VK031_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HLS53979.1"
                     /translation="MARVKRGANARRRHKKVLKQAKGYYGAKSKLFKPANQAVMKSLD
                     YAYIGRKQRKRDFRRLWITRINAATRANGMSYSRFINGLKKANIEINRKMLSEMAIHD
                     AEGFAKLVEIAKNN"
     gene            complement(1023..1220)
                     /gene="rpmI"
                     /locus_tag="VK031_08405"
     CDS             complement(1023..1220)
                     /gene="rpmI"
                     /locus_tag="VK031_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HLS53980.1"
                     /translation="MSKVKMKTHKGSAKRFKRTASGKIKRHKAYRSHLTGKKSPKRVR
                     NLRKSTLVSKGDQKRIDSMIP"
     gene            complement(1240..1752)
                     /gene="infC"
                     /locus_tag="VK031_08410"
     CDS             complement(1240..1752)
                     /gene="infC"
                     /locus_tag="VK031_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HLS53981.1"
                     /translation="MIKELQVNEGIREREVRLIDVDGEQLGIVPINQALDKAFERKLD
                     LVNVAPNARPPVCRIMDYGKYKYEMAKREKEARKKQRVINIKEIRLTPNIEDHDLNVK
                     ARQANGFLKSGDKVKVSVRFRGREMGHKNIGRTVLKNFADLTKDHGFVEKPARMEGRN
                     MTMFLAPKTE"
     misc_feature    complement(1772..1916)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2152..3705
                     /locus_tag="VK031_08415"
     CDS             2152..3705
                     /locus_tag="VK031_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLS53982.1"
                     /translation="MLKKKNVLLFFLILGMIASGLLILQYFLSIEGLEGIKTGEKLTV
                     WLITLTLLIYYIKYRSKPQLVPSILTIEKNNERNQDKPNVSFKDVAGLEEIKEELQET
                     IDFINNPLKYKKMGARIPKGILFHGPPGTGKTLLAKAVAGETNSSFLYASGSEFVEKY
                     VGVGAKRVRTLFEKAKKDTPSIIFIDEIDAIGTKRNMESNNEKDQTLNQLLVELDGFN
                     SDDTVVIIGATNRLDLLDEALLRPGRFDRHIYIGNPNMAAREKILQVHTSNKPLDGSI
                     NIKELAKKTTGLSGAQLANIANEAAIIAVRNNKSFISPTEFEAAIERVIAGLELKNPN
                     VLLKEKKIVAYHEAGHALVSKILNTDLVQKISIVPRGQALGYLLKYPDEDRYLLTKQE
                     LFNKIVCLLAGRASEEIIFKEVTTGAKDDLDKATAIAREMVCSYGMSSMGTLALDEVY
                     IRYNYDAIRLEIKKITDSAYQEAKEIIQENIRILKHISKTLMEKETIDNKELDQIFKL
                     YQVPCDYAT"
BASE COUNT         1119 a          707 c          609 g         1309 t
ORIGIN      
        1 taaatatgga acccatggtg gcccgcacca ccttaggatt ataagggtcc actgttcctt
       61 ttcctaaaat aatcccatct atattaaagg catctgcagt tctaattatg gttcctatat
      121 tgcctggatc ctggacctgg tctaggtata ctaaaaaggg ctggtctttt atacaaatat
      181 cttccagcct tctttctata aaagtagcta ctgccaagat cccttgaggg ctttctgtat
      241 ctgaaatttc ttcaaagagc ctatctgtaa ccttgatttt ttgataattc cctgccattt
      301 caattaaata agcagactct ttattttcta aaaagctttc cccaaaaact aaatatttaa
      361 gtttgatctg ttctgttaaa gcctcttcta tgattttagg tccttctatt ataaatagat
      421 tcttatccca tctatttttt cttctatata agctttttat ctctttgata atccggtttt
      481 gcggtgatgt aacctctaac ataccttcac cttccattta cttaaaatac ttgctaatat
      541 ggtgatttta tccagcctag ttttaattat actggcctct tttagatttt ctatttccct
      601 ataaaaaaag aaaaagttcc tatcaggaac tttagattaa ttattttttg caatttcaac
      661 tagttttgcg aaaccttctg catcatgaat agccatctct gatagcattt ttctatttat
      721 ttcaatattg gccttcttta gtccatttat gaatctacta tagctcatgc catttgctct
      781 agtagccgca ttgattctag tgatccacaa tcttctaaag tctcttttcc tctgttttct
      841 gccaatatat gcatagtcta atgatttcat aacagcttgg ttagctggtt taaacaattt
      901 gcttttggca ccataatatc cttttgcttg ctttaatact tttttatgtc tccttctggc
      961 atttgcgcct ctttttactc ttgccattgc tataactcct cctatatatt aaataatgat
     1021 ccttatggta tcattgaatc tattcttttt tggtcacctt tacttactaa ggttgacttt
     1081 cttaaatttc taactctttt tggtgatttt ttaccagtta agtggcttct gtatgccttg
     1141 tgccttttaa ttttgccact tgcagttctt ttgaaccttt ttgctgaccc tttatgggtt
     1201 ttcatcttta cctttgacat aatctatcct ctcctataat tactctgttt ttggtgctag
     1261 aaacattgtc atatttctgc cttccatcct agcaggtttc tccacaaaac catggtcttt
     1321 agttaaatct gcaaagtttt ttaatactgt tcttccaatg tttttgtgtc ccatttctct
     1381 acctctgaat ctgacagata cctttacctt gtcgcctgat tttaggaaac cattggcctg
     1441 cctggctttt acgtttaagt catggtcttc aatattaggg gttaagcgga tttctttaat
     1501 attaataact ctttgttttt tcctagcctc tttttccctt tttgccattt catacttgta
     1561 tttaccgtag tccattatcc tacatacagg tggcctggca tttggagcca catttaccaa
     1621 atctaatttt ctttcaaagg ccttatctaa agcctggttt atagggacaa ttcccaactg
     1681 ctcaccgtcc acatcaatta accttacttc cctttctctg atcccctcat ttacctgaag
     1741 ttctttaata attcagcacc tcctataaat atttaaaata aaaaagcgag tacaaaatac
     1801 ccgctttatc cacataacaa gctaaacctt acctagtaag atctacaccc atattcttgc
     1861 cattacctta tgagccctag ctataaggtg agaagcggac acttctactt gaattaacaa
     1921 tatttatttc accatgctat tataagccaa ataaaaggct ttgtcaaatc ttttatttcc
     1981 tatttagtct atattgtaaa ttattttcaa ataggcattt aaggccagtt taaattaaag
     2041 taaatatctt ccaactatat tatatactat tttcttgaaa aatttacttt aatttttata
     2101 ttaaaacatt aaatggatac actaaaacta gaatagattt ggggaggaga tgtgttgaag
     2161 aaaaagaatg tattgctttt ttttctaata ttgggaatga tagcatctgg tctgcttatt
     2221 ctccaatatt ttttatctat agaaggatta gaaggtatta agactggtga aaagcttact
     2281 gtctggttaa ttacattaac cctcttaata tattatatta aatatagatc taagccccaa
     2341 ctggtaccct ccatattaac tattgaaaaa aacaatgaaa gaaaccaaga taagcctaat
     2401 gtatccttta aggatgtggc cggtttggaa gagattaagg aagagttgca ggaaactatt
     2461 gactttatta acaatccttt aaagtataaa aaaatgggtg ctagaatacc taagggaatt
     2521 ctttttcatg gtcctcccgg tactggtaaa actcttttag ccaaggctgt ggcaggggag
     2581 accaattcca gttttttata tgccagcgga tctgaatttg tggaaaaata tgtaggtgtt
     2641 ggtgccaaaa gggttaggac actttttgaa aaggctaaaa aggatactcc cagcatcatc
     2701 ttcattgatg agattgatgc cataggtacc aaaagaaata tggagtctaa taatgaaaag
     2761 gatcaaacct taaatcagct cttagttgag ttggacggct ttaacagcga tgacacagtg
     2821 gtcataattg gagccaccaa tagattagat ttattagatg aggccctatt aaggcctggt
     2881 aggtttgata ggcacatata tataggaaat cctaatatgg ctgcccggga gaaaatcctt
     2941 caggtccata ctagtaacaa acccttagat ggaagtatta atattaagga actggctaaa
     3001 aaaaccactg gcctgtctgg tgcccagctg gccaatatag ccaatgaagc agccattatt
     3061 gctgtcagga acaataaaag ctttatttct cctactgaat ttgaggctgc cattgaaagg
     3121 gttatagcag gtttggaact taaaaatcct aatgtcctat taaaggaaaa gaaaatagta
     3181 gcctatcatg aggctgggca tgccttagta tctaagattt taaatacaga tttggtacaa
     3241 aaaatctcca ttgtgcctag gggccaggcc ttaggatact tgcttaaata tccagatgag
     3301 gatagatatc tacttacaaa acaagagcta tttaataaga ttgtttgtct tttggccggt
     3361 agagcatcag aggagataat atttaaggag gttaccactg gggcaaagga tgatctagat
     3421 aaggctacgg ccatagccag agaaatggtc tgtagctatg gcatgagttc tatgggaacc
     3481 ttggctctag atgaggttta tatccgctac aattatgatg ccattagatt agaaatcaaa
     3541 aaaataactg acagtgccta ccaggaggcc aaggaaataa ttcaggaaaa tataagaatc
     3601 ctaaaacata tttccaaaac cttaatggaa aaagagacta tagataataa ggagctggac
     3661 caaatcttca aattgtatca ggttccctgc gattatgcca cataaaaaat ataaaaggaa
     3721 gggttaaccc ttccttttat attt
//
LOCUS       DATGSI010000545         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_415637, whole genome shotgun sequence.
ACCESSION   DATGSI010000545 DATGSI010000000
VERSION     DATGSI010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415637"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VK031_08420"
     CDS             complement(<1..225)
                     /locus_tag="VK031_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53983.1"
                     /translation="MLDIDKNRLLLNLFQEDINENHKNTYISQLALITIKSLLPSSKL
                     AGNIIDYRRFNKELKLWKYYKLGQAGPLEEM"
     gene            complement(235..1935)
                     /gene="argS"
                     /locus_tag="VK031_08425"
     CDS             complement(235..1935)
                     /gene="argS"
                     /locus_tag="VK031_08425"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005955710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HLS53984.1"
                     /translation="MIDFKEKVIELISNEVDFLDRDEISNTIEIPPSPEMGNYAFPVF
                     RLSKVYRKAPNLIAEELVTKFGDNQYFEKIENVGPYINFFIDKSKLIETVLNEIMDKG
                     EKYGSSKMGEGKNVLIDFSSPNIAKPFHMGHIRTTVIGNALYKIYNFLGFNTVAINHL
                     GDYGTQFGMLISAYKKWGDRQVIEQDPINELLKLYVKYNEAAEEDEKLRDEARYWFKE
                     LENKNEEAVELWQWIRDISLKEFNRVYDMFKIKFDSYAGESFYADKTDEVVDKLKEKG
                     LLTESEGAYLVDLEEYDMPPALIMKSDGSTLYTTRDIAAAIYRKEKYDFYKNIYVVAS
                     QQNLHFRQLIKVLELMGYDWAKDCVHIPFGMVSLEDGPLSTRRGRVVFLEDVLNKAVE
                     NTLEIINERNPDLENKEEVAKQVGIGAVVFQELFNQRIKDYVFSWDRTLSFEGETGPY
                     VQYTHARANSLLERGKFNLDDTVDLSLLESEDEVNVIRLLYDFPRVVVDAMEKNEPYF
                     ITRHIVEIAKAFNKFYTNSQIIVEDEEIKRARLMLTYCTKTVIKVGLGLLGIESPNKM
                     "
     gene            complement(1987..>2368)
                     /locus_tag="VK031_08430"
     CDS             complement(1987..>2368)
                     /locus_tag="VK031_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HLS53985.1"
                     /translation="ENGNLNVQVGIMKVNVHINTLRRTEANIDEDLTKRTRNIITSKS
                     KTIKNEIDLRGSTIEEAILDLDKYLDDSYIAGLKEVYVIHGKGTGALRKGLQDYFKRH
                     RLIKSFRLGEYGEGGSGVTIVRLI"
BASE COUNT          644 a          496 c          325 g          903 t
ORIGIN      
        1 catttcctct aagggccctg cctgccctag cttataatat ttccacaatt taagttcttt
       61 attaaatctt ctataatcta ttatatttcc agctaacttg ctactaggca gtagtgactt
      121 tatggttatt aaggccaatt gggaaatata ggtgttttta tgattttcat taatatcttc
      181 ttgaaacagg ttcagtaaaa gtctattttt gtctatatcc aacatactat gctcctacat
      241 cttatttggg gattcaattc ccaataagcc taggcctacc tttataaccg ttttagtaca
      301 gtaggtcaac ataagtctag ccctttttat ttcttcatct tccacgatta tttgtgaatt
      361 ggtataaaac ttattaaagg cctttgctat ttctactata tgcctagtta taaagtaggg
      421 ctcatttttt tccatggcat ctactacaac tcttgggaaa tcatatagta gtctgattac
      481 atttacttca tcttctgact caagcaggct taagtctact gtatcatcta aattaaattt
      541 acccctctct aatagtgaat tggccctggc atgggtatac tgcacataag gtccagtttc
      601 tccctcaaaa cttaaggtcc tgtcccagga gaaaacatag tcctttattc tttgattaaa
      661 taattcttgg aaaactacag caccaattcc tacctgtttg gctacttctt ccttgttttc
      721 taagtctggg tttctttcat ttataatctc taaggtgttt tccactgcct tatttaaaac
      781 atcttctaaa aatactaccc tgcccctcct agtggatagg ggaccatctt ccaggcttac
      841 catgccaaaa ggaatatgga cacaatcttt ggcccaatca tagcccatta attctagaac
      901 ctttatcaat tgtctaaaat gtaaattctg ttgggatgcc accacatata tgtttttata
      961 gaagtcatat ttttcctttc tataaatggc cgccgctata tctctggttg tatatagggt
     1021 agagccatca cttttcataa ttagggccgg tggcatatca tattcttcta gatctactaa
     1081 ataggctccc tctgattctg ttaataggcc tttctctttt aacttatcta ctacttcatc
     1141 tgttttatct gcatagaagc tttctccagc ataggaatca aactttatct taaacatatc
     1201 atatactcta ttaaattcct tcaggctaat atctctaatc cattgccaaa gctctacagc
     1261 ttcttcattt ttgttctcta actctttgaa ccagtacctg gcctcatctc ttaatttttc
     1321 atcttcttct gctgcctcat tgtattttac atatagctta agcagttcat ttattggatc
     1381 ctgctctatt acctgcctgt ctccccactt tttgtatgca gatattaaca taccaaattg
     1441 ggttccataa tcacctaaat ggtttatggc tacagtgtta aatcctaaaa agttataaat
     1501 tttgtatagg gcattaccta taactgtggt tctaatatgg cccatatgga aaggcttggc
     1561 tatgttaggt gatgaaaaat ctattaaaac attttttcct tctcccatct tggaagatcc
     1621 gtatttctcg cctttatcca ttatttcatt taaaactgtc tctattaact tggatttatc
     1681 aataaaaaag ttaatataag ggcctacatt ttcaattttt tcaaagtact gattgtctcc
     1741 aaattttgtt accaattctt ctgctattag gtttggtgcc ttcctataga ccttgcttag
     1801 tctaaagaca ggaaaagcat aattccccat ttcaggagag ggagggatct ctattgtatt
     1861 ggatatttca tccctatcta agaaatcaac ttcatttgat attagctcta ttactttttc
     1921 cttaaagtct atcattttaa acctcctagt tcttatccaa atgtatatta aaatttttaa
     1981 ttataactaa attaacctta ctatggttac accgctgcca ccttcaccgt attcacctaa
     2041 acggaaggat ttgatcagtc tatgcctttt aaaatagtcc tgaaggcctt ttcttagggc
     2101 accagttcct tttccatgaa ttacatacac ttcctttagg ccggctatat aagaatcgtc
     2161 taaatattta tctaaatcta agatggcttc ctctatggtg ctacccctga gatcaatttc
     2221 attctttata gttttagatt tgctagttat gatgtttcta gtccttttag taaggtcctc
     2281 gtctatattt gcctctgttc ttcttaaggt attaatatga acatttacct tcattatacc
     2341 cacttgtaca tttaaatttc cattttca
//
LOCUS       DATGSI010000546         5634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_269184, whole genome shotgun sequence.
ACCESSION   DATGSI010000546 DATGSI010000000
VERSION     DATGSI010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269184"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VK031_08435"
     CDS             complement(<1..615)
                     /locus_tag="VK031_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HLS53986.1"
                     /translation="MANLMIQGTTSSAGKTLISTVLCRIFKEDGYSVYPFKSQNMTSK
                     FYETKEGYKLSPAQVLQAKAAGKIPNPNMNPILLLPVTNQGSRVILLGEEKYQMEAWE
                     YFKFRKSLKGFISTIYKEIEGENDIVIIEGAGSPAEINLKENDIVNMGMAKIADAPVL
                     LVADIDRGGVFASLYGTVMLLEEEERERIKGLIINKFRGDKSLLD"
     gene            complement(654..1550)
                     /locus_tag="VK031_08440"
     CDS             complement(654..1550)
                     /locus_tag="VK031_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLS53987.1"
                     /translation="MFSHIGELAALGTALGYTLNGIYFEAAGKRVGSLAVNFIRLVIG
                     FIFLSLFTYFTRGYLLPTDSTLHNWIWLGISGFIGFFLGGIFLFQAYVEIGTRITLLI
                     LGVSPPITALLGYIFLKETISIMGIIGMFVTIAGIALVILGKDTDEEKIEFKHPIKGI
                     FFAFLGALGNSIGMIFSKIGMQDYHPLAATQIRIVIGFISFVILFAYLNKWSVLRKAI
                     KDREAMKKITLGSLFGPFLGVSLQLTSLKYTTAGVSATITSIMPITVLPHSILVEKEQ
                     INAKEIIGTLLSVAGIAILFLI"
     gene            complement(1579..1854)
                     /locus_tag="VK031_08445"
     CDS             complement(1579..1854)
                     /locus_tag="VK031_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HLS53988.1"
                     /translation="MKNSYRFFNNKDCQFLPCHEVKDVDKFNCMFCYCPLYFLEDCGG
                     NYKDNHGIKDCSDCLIPHGPKGYDIINKKIMEINEKKVTERLLATEE"
     gene            complement(1986..2423)
                     /locus_tag="VK031_08450"
     CDS             complement(1986..2423)
                     /locus_tag="VK031_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53989.1"
                     /translation="MSNLDLDQLIQDLSEKISNINLDDKGINQLIEEVKEKIESNQLY
                     LSISDDVKTTLEMVTDWREGKYTDLSQNTIFIVLAGLFYIVNPLNILPKLFKDSILDE
                     IIVMGYILKKVRDELGEYRKWKENQDWQEDWTEEDDTVYIEIK"
     gene            complement(2560..3426)
                     /locus_tag="VK031_08455"
     CDS             complement(2560..3426)
                     /locus_tag="VK031_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HLS53990.1"
                     /translation="MKRRRFVVSTVLILTLILTFNFIAHADSAVVVTLAKDLNDKQRA
                     DILELFNVKEDEVNIIEVNNQEERRYLEGIAPEEQIGNITMSSAYVELLEAGSGIDVQ
                     TFNISWVTEDMYKGALVTAGVKDAKVMAAAPFSVSGTGALTGIIKAFEQATGEVIDEE
                     QKRVANEEIVQTGELGEEIGKDKASELINKVKEEVVDKGVKSPEDIKKIIIEIAASLD
                     INLNDEQLAKLTQLMEKISKLNINTDDIKEQLKGIGEKLDDVLKDNQEVKSILQKILD
                     AIKNFFKSIFGK"
     gene            complement(3587..4231)
                     /gene="pcp"
                     /locus_tag="VK031_08460"
     CDS             complement(3587..4231)
                     /gene="pcp"
                     /locus_tag="VK031_08460"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HLS53991.1"
                     /translation="MKILVTAFDPFGGDKINPALEAVKLLKEEIAGAEIVKLMIPTVF
                     KKSVEKTVEAIEKERPDVVLSVGQAGGRFDLTPERVAINIDDARIKDNEGNQPIDQPI
                     YKDGKPAYFSTLPIKAMVENIRKVGLPASVSNTAGTFVCNHIMYGVLYHIDKTGKDIR
                     AGFIHVPFTPEQVVSRPSPAPYMATEDIARGLEAAIEAIVENDQDIKSIGGKIH"
     gene            complement(4249..5178)
                     /locus_tag="VK031_08465"
     CDS             complement(4249..5178)
                     /locus_tag="VK031_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HLS53992.1"
                     /translation="MDLNTLIGEIFFSIIGIIFIICGIYALRDKELENRIPTAIFWFI
                     LAFTFIVGPYIPNWITGVCILIIAGITAFDKVQPAKTDEVTEEETRKNADKLGYKVFI
                     PVISLAIIAVVVAYILVPLNLSTNHAIGISAVFALIITFIITKAPGKYAVSDGSRLMM
                     NVGPVGILPQLLAALGALFTAAGVGDVIANGVSAVIPMGNKLLGVVAYCIGMALFTII
                     MGNGFAAFSVITVGIGIPFVISQGADPIVVGALGLTAGYCGTLLTPMAANFNIMPAAL
                     LETKDKNAIIKAQAPFAITMLIIHIILMYIFAF"
     gene            complement(5195..>5634)
                     /locus_tag="VK031_08470"
     CDS             complement(5195..>5634)
                     /locus_tag="VK031_08470"
                     /inference="COORDINATES: protein motif:HMM:NF017916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF969 family protein"
                     /protein_id="HLS53993.1"
                     /translation="FIGGIKGATTGKVLSLYQTIRAISAAFSVRLGGHPQFIRPLINP
                     MSQAAAIARYGDLDEDTEDQIKGAAAASENYGNFFAQNVFMGASGTLLIVSTLSEQGY
                     AEVTPLNIAMWSIPVAVISVIVGIIQNRWLDIRLAKKYGNRDK"
BASE COUNT         1647 a         1186 c          815 g         1986 t
ORIGIN      
        1 atctaaaagg cttttatccc cacgaaactt atttattatt agacccttta ttctttctct
       61 ttcttcctcc tccagcaaca taactgtgcc gtaaagggag gcaaacactc ctcctctatc
      121 tatatcagct accagaagaa ctggggcatc tgctattttc gccataccca tattgacaat
      181 atcattttcc ttcaaattta tctctgcagg acttcctgcc ccttctataa ttacaatatc
      241 attttctcct tctatttcct tatagattgt actaataaac ccctttaaac ttttcctaaa
      301 cttaaaatat tcccaagcct ccatttgata cttttcttca cctagtagaa ttaccctgga
      361 tccttggttg gtaactggca acaataggat aggattcata ttaggattgg gaattttccc
      421 tgctgccttt gcttgtaata cctgggctgg actaagttta taaccttcct ttgtttcata
      481 gaatttagag gtcatattct gcgacttaaa aggatacaca ctatagccat cctccttaaa
      541 tattctacat agaacagtag atattaaggt tttccctgct gaggatgtgg tgccttgaat
      601 cattaaatta gccatatcat tctccttata aatttaattt tataggtatt tagttagatt
      661 aaaaataaaa ttgcaatacc agctactgaa agcaaggttc ctattatctc tttagcattt
      721 atttgttctt tctctaccaa tatggagtgg ggaagaacag ttatgggcat aatggaagta
      781 attgttgctg atactcctgc tgtagtgtat tttaaagatg taagctgtaa agacacccct
      841 aagaaggggc caaatagtga gcctagggta atctttttca tggcttccct atctttaatg
      901 gccttcctta agactgacca cttatttaaa taggcaaata atatgacaaa ggaaataaag
      961 cctatgacaa tccttatttg agttgctgcc aagggatggt aatcctgcat acctattttg
     1021 ctaaagatca ttccaataga atttcctagt gctcctaaga aggcaaaaaa tatacctttt
     1081 atagggtgtt taaattctat tttttcctca tctgtatcct tgcctaatat taccaaggca
     1141 attccagcta tggttacaaa cattccaatt atccccataa tagatatggt ttccttgaga
     1201 aatatatagc ctaaaagggc agttattgga ggacttactc ctaaaatcag taaagttatt
     1261 ctggtgccta tttctacata ggcttgaaac aaaaatatac ctcccaggaa gaatccgata
     1321 aagcctgata tccccagcca gatccaatta tgcaaggtgg aatctgtggg caaaagataa
     1381 cctcttgtaa agtatgtaaa taggcttaaa aaaataaaac ctattactag tctgataaaa
     1441 tttacagcca aggagccaac tctttttcct gccgcttcaa aatagattcc atttaatgta
     1501 taccctaagg ccgtacctag ggcggccaac tcacctatat gagaaaacat aactttactc
     1561 cttttcaagt tattattatt attcttctgt agccaataat ctttctgtca cctttttttc
     1621 atttatctcc attatcttct tgtttataat gtcataaccc ttaggtccat gggggattaa
     1681 acaatctgaa cagtccttta ttccatgatt gtccttataa ttcccaccac aatcctctaa
     1741 aaagtataag gggcaatagc aaaacataca attaaacttg tccacatcct tcacctcatg
     1801 acagggaaga aattggcaat ccttgttgtt aaaaaatctg taagaattct tcatatatat
     1861 cgtcctttct tatgtataag tttaaagtaa ggataaaagg ctagcctttt atccttactt
     1921 taactgctga agatttacct ataaaataag ttttaaaact tactttaagt ttaagaagaa
     1981 cctgcctact ttatttctat atatacagta tcatcctctt ccgtccagtc ttcttgccag
     2041 tcctgatttt ctttccattt cctatattcc cctaattcat ctcttacttt tttcaatata
     2101 taacccatta ctattatttc atccaggata ctatctttaa aaagtttggg cagaatattt
     2161 aatggattta ctatataaaa taggcctgcc aatacaataa atatagtgtt ctgggatagg
     2221 tcagtgtact tgccttcccg ccaatctgtt accatttcaa gagtagtttt tacatcatct
     2281 gatatgctta aatataattg gttggattct attttttctt taacttcctc aatcaattga
     2341 tttattcctt tatcatcaag atttatattg ctaatttttt cacttaagtc ttgaattaat
     2401 tgatctaaat ctaaattact catatcaaca cctcctggga ccagctaaca catgataggt
     2461 cttttatagc tatagtatta ttatagcaaa taattttcta aatagttaaa aaaggtcata
     2521 aatatgacct tttttaatct tacctatatt attttatttt tactttccaa agattgattt
     2581 gaagaaattc ttaatagcat ctagtatttt ttgtaaaatt gattttactt cctggttgtc
     2641 ctttaagaca tcatctagtt tttctcctat gccttttaat tgctccttaa tatcatcagt
     2701 atttatattt agtttgctga ttttttccat taattgtgtt aattttgcca attgttcatc
     2761 atttagattg atgtctaggc tagccgctat ttctataatt attttcttta tatcttctgg
     2821 actcttaact cccttgtcta ctacctcttc ctttaccttg tttattaact cactggcctt
     2881 atccttccct atttcttcac ctaattcacc agtttggact atttcttcat tggctactct
     2941 tttttgttct tcatctatta cttctccagt tgcctgctca aaggccttga ttattccagt
     3001 taaggctccg gtaccagata ctgaaaaggg ggctgcagcc ataaccttag catccttaac
     3061 tccagcagtt accagggccc ctttatacat atcttcagtt acccatgaaa tattaaatgt
     3121 ttgcacatca ataccagaac ctgcttctaa aagttccaca taggcagagg acatggttat
     3181 attgcctatt tgctcctcag gtgcaattcc ttctaggtat cttctttctt cctgattatt
     3241 tacttctatt atatttacct catcttcttt tacattaaat agctccaata tatctgccct
     3301 ttgtttatca tttaaatctt tggccaatgt taccactact gcgctatctg catgggcaat
     3361 gaaattaaat gttagtatta aagtcaagat tagaactgta ctaaccacaa atcttcttct
     3421 tttcattttt acaccatcct tattttaaag taactatatt tatatcttac catattagat
     3481 ttatttttaa tagttacaat ccagttaagc ataggataaa atagggaaaa taaaagcgga
     3541 tgtattaagc tacatccact ttttcttcca tttactttaa taatttttaa tgaatctttc
     3601 cacctatact tttaatatct tggtcatttt caactatagc ctcaattgct gcttctagcc
     3661 ccctagctat atcttccgtg gccatatagg gagctgggct aggtcttgat accacctgct
     3721 ctggtgtaaa gggcacatga ataaatcctg cccgtatatc ttttccagtc ttatcaatat
     3781 ggtacaaaac cccatacatt atatggttac agacaaaggt tcctgctgta tttgaaactg
     3841 aggcaggtaa tcctactttc cttatatttt ccaccatggc cttaataggt aaagtggaga
     3901 aataggctgg cttcccatcc ttataaattg gctgatcaat aggctgattg ccctcattat
     3961 ccttgattct tgcatcatct atgtttattg ccaccctttc aggcgttaaa tcaaacctac
     4021 ctccagcctg acctacagat aataccacat caggcctttc tttttctata gcctctactg
     4081 ttttctctac agatttttta aaaacagtag gaatcataag ttttacaatt tccgctcctg
     4141 caatttcttc ctttaataat ttcacagcct ctaaggctgg atttatcttg tctcctccaa
     4201 agggatcaaa ggctgtaact agaattttca ttttcaaacc tccttacatt aaaaggcaaa
     4261 tatatacatt aaaattatat ggataattaa catggttatg gcaaaggggg cctgggcttt
     4321 aattattgcg tttttatcct ttgtttctaa tagggcagcc ggcataatat tgaaatttgc
     4381 agccatcggt gtaagaagcg tcccacagta acctgcagtc aggcctaagg ctccaacaac
     4441 tatagggtct gctccctggg agataacaaa aggaatccct atacctactg taattacaga
     4501 aaaagctgca aagccattcc ccattataat ggtaaatagg gccatgccta tacagtaggc
     4561 cacaactccc aatagcttat tacccatagg aataactgct gacacaccat ttgcaattac
     4621 atctcctact cctgctgccg taaataaggc tcccagggct gccaaaagtt gaggcaggat
     4681 acctactgga cctacattca tcattaaacg gctaccatca cttacggcat acttaccagg
     4741 tgctttagtt ataatgaagg taatgataag tgcaaagact gctgaaatac caatggcatg
     4801 attggtactt aaatttaatg gtaccaatat ataggccact actactgcta taatggccag
     4861 tgatattact gggataaata ccttatagcc caacttatcg gcatttttcc ttgtttcttc
     4921 ctcagtcact tcatcagttt tggcaggttg aaccttgtca aaggctgtta tacctgcgat
     4981 aattaatatg caaactccag ttatccaatt tggtatataa ggtcctacta taaaggtaaa
     5041 ggccaaaata aaccagaata ttgcagttgg tattctattt tccaactcct tgtctctcaa
     5101 agcataaatt ccacaaatta tgaatattat tccaattatg gagaaaaata tttctcctat
     5161 tagggtattt aaatccatta tcattcacct acctttactt atctctatta ccatattttt
     5221 tggccagcct tatatctagc catctattct gaattatacc tactattact gaaattacag
     5281 ccactggaat agaccacatg gctatgttta aaggggttac ctctgcatat ccttgctctg
     5341 ataaagtact tactattaat aaggttcctg aagcccccat aaacacattt tgggcgaaga
     5401 aatttccgta attttctgaa gctgctgccg ctccttttat ttggtcttca gtgtcctcat
     5461 ctaagtcccc gtacctggct atggctgcag cctggctcat agggttaatt aaaggtctta
     5521 taaattgagg atgacctcca agtcttactg aaaaggctgc tgatatggcc cttatagttt
     5581 ggtataaaga caatacctta ccagttgtag ctccctttat acctcctata aagt
//
LOCUS       DATGSI010000547         1677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_35181, whole genome shotgun sequence.
ACCESSION   DATGSI010000547 DATGSI010000000
VERSION     DATGSI010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35181"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..969)
                     /gene="glyQ"
                     /locus_tag="VK031_08475"
     CDS             complement(82..969)
                     /gene="glyQ"
                     /locus_tag="VK031_08475"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HLS53994.1"
                     /translation="MYFQDLMLKLLNYWGEKGCLIMEPYDIEKGAGTMSPHTFLRALG
                     PEPWKVVYVEPSRRPADARYGQNPNRVYQHHQLQVILKPSTENIQDLYLDSLKAIGID
                     PLKHDIRFVEDNWEAPTLGAWGLGWEVWLDGMEITQFTYFQQVGSINCELESAEITYG
                     LERIAMYLQDVDNIFDIKWNKDITYGDIFNRAEFEHSVYSFEKANVETLKDLFNIYET
                     EAKRIIEKNLVLPAYDFVLKCSHTFNVLDARGAISVSERTNYIGRVRSLARDIAANYL
                     GQREELGLPLIEKVGEINE"
     misc_binding    complement(1012..1185)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1248..>1677)
                     /gene="recO"
                     /locus_tag="VK031_08480"
     CDS             complement(1248..>1677)
                     /gene="recO"
                     /locus_tag="VK031_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HLS53995.1"
                     /translation="NERIFLLLLKGLEVLSKLDEGFLKFTYAFGLKYISFLGYRPYID
                     KCVICNEIPFGKFKFSHIEGGIICRNCFDQDLNAKSMTSDMYKDMQEILYTPLDDLDK
                     LDIKESNLKRLQNILEEYILYSIDRKSFNSLKLIRSIEYK"
BASE COUNT          511 a          367 c          215 g          584 t
ORIGIN      
        1 aatttttcct ttatctgtct acaggcccca tctatatacc tggccggtaa ttcttccaca
       61 cctatttcaa gtaaataatt tttactcatt aatttcacct actttttcaa ttaaaggtag
      121 tcctaactct tccctttgcc ctaagtaatt ggcggctatg tctcttgcaa gactccttac
      181 cctgcctata taattggtcc tttctgatac agatatggct cctcttgcat ctaatacatt
      241 aaaagtatgg gagcatttta ggacaaaatc atatgcaggt aagactaagt ttttctctat
      301 aatccttttg gcctcagtct catagatatt aaataaatcc tttaaggttt ccacattggc
      361 cttctcaaaa ctatagacag aatgttcaaa ctccgcccta ttgaaaatgt caccataggt
      421 tatatcctta ttccacttta tatcaaatat attatcaaca tcctgtaggt acatggctat
      481 tctttctaaa ccataagtta tttcagctga ctctaattca cagttaatac tacctacctg
      541 ttgaaaatat gtaaattggg tgatttccat tccatctaac caaacttccc atccaaggcc
      601 ccaggccccc aaggtaggtg cctcccagtt gtcttcaaca aacctgatat catgttttaa
      661 aggatctata cctatggcct ttaaactatc caaatataaa tcttgaatat tttctgttga
      721 aggttttagt ataacctgca attggtgatg ttggtagacc cgattagggt tttggccata
      781 tctggcatca gcaggccttc ttgaaggctc cacataaact accttccacg gttctggccc
      841 caaagccctt aaaaaggtgt gagggctcat agttccagca cccttttcta tatcataggg
      901 ctccataatt aaacatccct tttctcccca gtaattcagt aacttcagca ttaagtcctg
      961 aaagtacata ttttcatcct cctttataat acaaaaaaac cttatctccc ctatagggac
     1021 gaaaggttaa ttccgcggtt ccaccctatt taataccata tcggtattct ctttaggtta
     1081 gatactcaga agctcctttc aatcatatat ttatatctag ttccacctaa cctagactct
     1141 ctataataaa taactatgat ctactctctt cctcatcgta ctaatattta gctatcttct
     1201 ttataattta gcaaataata catttaatgt caatagatac aagcctgcta cttgtattct
     1261 atacttctaa ttaattttaa agaattaaaa cttttcctat caatactata tagtatatat
     1321 tcttctaaaa tgttttgaag ccttttaaga ttggactctt ttatatcaag tttgtctaaa
     1381 tcatctaaag gagtatataa aatttcttgc atatctttat acatatcact tgtcatactt
     1441 ttggcattta agtcctgatc aaaacaattt ctacaaatga taccgccttc tatatggcta
     1501 aacttaaact tgccaaaggg tatctcatta cagattacac acttgtctat atagggccta
     1561 tatccaagaa aggatatata ttttaagcca aaggcatagg taaactttag aaagccctca
     1621 tccaacttgg ataagacttc tagtcccttt aaaagtaaaa gaaaaatcct ttcattt
//
LOCUS       DATGSI010000548         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_699788, whole genome shotgun sequence.
ACCESSION   DATGSI010000548 DATGSI010000000
VERSION     DATGSI010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699788"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VK031_08485"
     CDS             <1..811
                     /locus_tag="VK031_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53996.1"
                     /translation="EQRAEDRRMARLLEHEAKLLDMEDEFEAEQEMLDFQREQELLRL
                     GEEHEQGLLSEENYLKAVENLNKQHAEAQKEIDQAIHDNKIMLASKTLASLATIMGEE
                     TQAGKFFASAQALVDTYSAATSAYKAMAGIPVIGPALGAAAAGTAVATGLRNIQKINQ
                     VRTQKYTGGPTPDGGMFTRTGYVHAGEVVFSQQDVRQMGGADAVERLRPTSNQFGKMP
                     TGLSGDNDNMINWRQIASIMGQEVRRGSQIGTGSGIIEANDNAEVRRSAQF"
     gene            817..1050
                     /locus_tag="VK031_08490"
     CDS             817..1050
                     /locus_tag="VK031_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53997.1"
                     /translation="MNQKAILIGLKNYNKDTDVNKVRLEKIANCKHAVEAPIKALRLS
                     DRYDELNNKICNKCYCPLPTKARQEIEKCECWM"
     gene            1345..1527
                     /locus_tag="VK031_08495"
     CDS             1345..1527
                     /locus_tag="VK031_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53998.1"
                     /translation="MISSEAVYKNGRNRLEKNWNEGGNILLLIYIISGAILSAGVYVA
                     LLKLNILINVNDYLTK"
     gene            1536..1748
                     /locus_tag="VK031_08500"
     CDS             1536..1748
                     /locus_tag="VK031_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS53999.1"
                     /translation="MEIEDRIKQIKDINKKIEYLSDIKRDLSRIEKEGSARHISLRLF
                     NNTINISNPDFVKTISVLTIDYLKRV"
BASE COUNT          798 a          289 c          420 g          621 t
ORIGIN      
        1 tgagcaacgt gcagaagata gaagaatggc taggctactt gagcatgagg ctaaacttct
       61 ggacatggaa gatgagtttg aagctgaaca agaaatgcta gacttccaaa gagaacagga
      121 gttattaaga cttggtgagg aacatgagca aggtttgtta agcgaagaga attacttaaa
      181 agcagtagag aatctaaaca agcaacatgc agaagctcag aaggaaatag accaagctat
      241 acatgataac aagattatgt tagcgtctaa gacactagct agtctagcta ctataatggg
      301 tgaagaaaca caagctggca agttctttgc atcagcacaa gcgttagtag acacctactc
      361 agctgctaca tctgcttata aggctatggc tggtattcct gttattggtc cagcattggg
      421 tgctgctgct gcaggtactg cagttgctac tggacttaga aatattcaga aaataaacca
      481 agttagaaca cagaaataca caggtggacc aactcccgat ggaggtatgt ttactagaac
      541 tggttatgtt catgctggtg aggtagtgtt ttcacaacaa gatgttaggc aaatgggagg
      601 tgctgatgca gtggaaaggt tacgacctac tagtaaccaa tttggaaaaa tgcctacggg
      661 actaagtggt gataatgata acatgattaa ttggcgacag atagcatcta ttatgggtca
      721 agaagtaagg cgtggtagcc aaattggaac aggtagtgga ataatagaag ctaatgataa
      781 tgctgaggta agacgaagcg cacaatttta aaagatatga atcaaaaagc aatattaata
      841 ggacttaaga attataataa agatactgat gttaacaaag tcagattaga aaagatagct
      901 aattgcaaac atgcagtaga agcacctata aaagcgttaa ggcttagtga tagatatgac
      961 gaactaaata ataaaatatg caacaaatgt tattgtccac taccaacaaa agcaagacaa
     1021 gaaatagaga agtgcgaatg ctggatgtag attatattag taagctgtat gaatctggaa
     1081 tagtaacaag ggcttcactt gatcgtgcta atataattaa tgactattat gctatgcgta
     1141 gcaagtttag acgtgtagag cttttatatt ataagcttgg cgagatacac cataaatcac
     1201 attggacgat tagaaatata gtacttgctt atgaaaggag tttgtagtat atttgtgact
     1261 aactaaataa ttatagatat tacaaatttt cttattatcc taggggtaac tcaatctctt
     1321 ttattaatac attggctata cgtgatgatt agttcagaag cagtgtataa aaatggtaga
     1381 aacagacttg aaaaaaactg gaatgaaggt ggtaatattt tactgctaat atacattata
     1441 tctggtgcga ttctttctgc aggagtgtat gtagctttat tgaagctaaa tattttaatt
     1501 aacgtaaatg attatttaac taaatagata tagagatgga aatagaagac agaataaaac
     1561 agataaaaga tataaataaa aagatagaat acttatctga tattaaaaga gatttatcta
     1621 ggattgaaaa agaaggtagt gcaaggcata taagtctaag gttatttaat aacactataa
     1681 atattagcaa tcctgatttt gtaaaaacaa ttagcgtatt aactattgat tacttaaaaa
     1741 gagtataaca tgatttagag gaagaaataa aaaactttca ataaacaacc ctctttattc
     1801 tctagtttgt ttaacccatt actttaatta gtagtgggtt tctttatatc tatatactat
     1861 taattatacc aatcgtaaca gcttaaccca caaatataca aaactactat tactatacta
     1921 ctctatattt tatatttatt tattttcgat aaaaagagta tggtagtagt atagcaacat
     1981 gttgcattat aattattcga cacgtgtaac ttattgatag tcagctccgt gacgcattga
     2041 cgcataactt tctaaaaagt tgtcagaaaa taaataggct tcttttattt ctagagagag
     2101 agtataggta aacactaaaa ccgtcatt
//
LOCUS       DATGSI010000549         4888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_460957, whole genome shotgun sequence.
ACCESSION   DATGSI010000549 DATGSI010000000
VERSION     DATGSI010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460957"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..209
                     /locus_tag="VK031_08505"
     CDS             48..209
                     /locus_tag="VK031_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54000.1"
                     /translation="MKDLKIRVLSGIIGILLLFFLVNKGGLYLALSICLLSLIGLNEF
                     YKAIKNMGH"
     gene            213..824
                     /locus_tag="VK031_08510"
     CDS             213..824
                     /locus_tag="VK031_08510"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HLS54001.1"
                     /translation="MQNIGYIATLGIFLSSFFERISVAFILSAVMISMLTILVFKKST
                     KVIDIGLTLLSVLYIPFFLFHIYYLDGSKYIWLVFIIAFGTDTFAYLSGNLLGKRKLS
                     PNISPNKTIEGSLGGIMGSVFCTTVYSIYYKLSPLWKLALLAVIASIFSQIGDLVASK
                     IKRWAKIKDYGNIMPGHGGVLDRFDSIIFSSPIIYYYIKLFII"
     gene            841..1848
                     /gene="rseP"
                     /locus_tag="VK031_08515"
     CDS             841..1848
                     /gene="rseP"
                     /locus_tag="VK031_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HLS54002.1"
                     /translation="MQTAIAAIFVFLLVILLHELGHFAVAKFVGIKVNEFAIGMGPKI
                     FQTKKGETKYTLRALPIGGYVNMEGEDEASDDPRGFSQASPLARMAVIAAGALMNFVL
                     AIIVLSIVSFNIGEPTTIIGEIQPGSPAEIAGIEPGDKIVNINKTKIKTWEDLSTTIA
                     GTSPEEEMEIVLIREEGEKILHVRPIVEEGRSLIGIIPEYESSLTLAIKGGFEKTAAF
                     IRLMFQFVGMIFRGQVSTQDLAGPVGVINEVGQAAKMGFTSLLLILGFISVNLGFFNL
                     LPIPALDGSRLVFIFIELIRGKPIDPEKENFIHLVGFAFLIGLMLVVTYRDLIRINIL
                     K"
     gene            1858..2913
                     /gene="ispG"
                     /locus_tag="VK031_08520"
                     /gene_synonym="gcpE"
     CDS             1858..2913
                     /gene="ispG"
                     /locus_tag="VK031_08520"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HLS54003.1"
                     /translation="MKRKTRVIKLGEVPIGGDNPITIQSMTNTITADTDSTIAQIKDL
                     EAAGCDIVRVAVANIDDAQAIKDIKAQINIPIIADIQYNYRLALESIKYGVDGLRINP
                     GNIGSLTRVKEIVKSCKERSIPIKIGVNSGSIKQEYLEKYNGVNAKSMVYSALEQIKL
                     LEELDFYDIKISLKASDVPLTIESYTMMAEMRNYPLHLGITEAGPAWRGTIKSAVGIG
                     SLLSKGIGDTIRISLTADPVEEIKVGREILKSLGLLNQGLEIISCPTCGRTRINLMEI
                     VEEAEKRLENIKGHMKVAIMGCPVNGPGEAREADIGIAGGNGKGIIFSKGQIVKRVRE
                     EDLLDELIKEVKKIDNL"
     gene            2944..4086
                     /locus_tag="VK031_08525"
     CDS             2944..4086
                     /locus_tag="VK031_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HLS54004.1"
                     /translation="MKRIKLASLVKDEKDSQLGELDIDIVKVEVVRELMEINLFLEAK
                     EIIDLRQQEKIEQFLSEKFPLFKVKTKIKYSLNKEQLEKVMGSYIKNLGLIIQEGIPS
                     ANSWLDKLDYKVNEGTLTLILPNELALFSMNRNGLVSLLKNKVQTELKLQLEIIKDDK
                     KEVEKVDFLEDQNIEERKISKDVLKNTKKTSRNKGTQARTDLLYGKRIKGSPIKINEV
                     NLQTGSAVIRGKIFQIESRSIRGNRSLILFFISDGTESIQVKLFVSKEEGLKLLSQLQ
                     EDMSLLIEGDIVYDNFSKSQVMMLKSAMVVNEEIRMDKAKNKRVELHLHTQMSSMDGV
                     ASLKKLAERAHQWGHKALAITDHGVVQAFPEGMELRKIRNKNDLWS"
     gene            4070..>4888
                     /locus_tag="VK031_08530"
     CDS             4070..>4888
                     /locus_tag="VK031_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HLS54005.1"
                     /translation="MIYGVEGYLVNDQKEIISNFNPDKEYEEFIVFDIETTGLSAIND
                     AITEIGAVKIRDGQVVDTFAQLINPERPIPDFIKELTGITDDMVRDKPTIDQVIPKFS
                     EFIGQGILVAHNASFDVGFIREKMRLNNISLNNPVLDTLELSRIVFPELKSFRLNNLA
                     KHLGVSLENHHRALDDAMATKDIFIKILELLEERNISNLKDINQLGAKGNINKDRPFH
                     VTILAKNQTGLKNLYRLISDSHIKHFYRRPRIPKSLLEEHREGLIVGSACEAGEL"
BASE COUNT         1726 a          669 c         1060 g         1433 t
ORIGIN      
        1 ccataattga ttaccaagct cgggatagaa gattcggagg tatataattg aaagatttaa
       61 aaattagagt cttaagcggc ataattggca tattgctatt attttttcta gtaaacaagg
      121 gtggtttata cctggcccta tccatatgcc tattatccct tattggatta aatgaatttt
      181 ataaggcaat aaaaaacatg ggacactagc caatgcaaaa tataggatat attgctactc
      241 ttggcatatt tttatcatcg ttttttgaaa ggatctctgt agcatttatt ttatcagctg
      301 taatgataag catgctaacc attttagtct ttaaaaaaag tacaaaggtt atagacatag
      361 gtcttacttt gttaagtgtt ttgtatatac ctttttttct ttttcatata tattacttgg
      421 atggcagtaa atatatatgg ctggttttta taattgcttt tggaacagac acctttgctt
      481 atttaagtgg gaacctatta ggaaaaagga agctatcccc aaacattagc ccaaataaaa
      541 ctattgaagg ctcattaggt ggtataatgg gaagtgtttt ttgcactact gtttattcca
      601 tctattataa attaagccct ttgtggaaac tagccctact ggcagtgatt gcctcaattt
      661 tttcacagat tggtgattta gttgcatcga aaataaaaag atgggctaaa ataaaagatt
      721 acggcaatat aatgccaggt cacgggggag tacttgatcg ctttgatagt attatttttt
      781 cttctcccat aatatattat tatataaaat tgtttataat ctaaatttgg tggtgataat
      841 atgcaaacgg ctatagcagc aatatttgtc tttttactag taatattgct acatgagttg
      901 ggccattttg ctgttgcaaa atttgttggt attaaggtaa atgaatttgc cataggaatg
      961 ggacctaaga tctttcaaac aaaaaaaggg gagactaaat atactctcag ggccttgcct
     1021 attggaggat atgtaaatat ggagggagaa gatgaggctt ccgatgatcc tagagggttt
     1081 agtcaggcct ctcccttggc cagaatggcg gtgatagctg ctggtgccct tatgaacttc
     1141 gtcctggcca taattgtatt atcaatagta tcctttaata taggtgagcc aactacgatt
     1201 ataggggaga ttcagcctgg atcacctgct gaaattgcag gaatagaacc aggggataaa
     1261 atagttaata ttaataaaac aaaaattaaa acctgggagg atttatcaac cactatagcc
     1321 ggcacttcgc cagaggagga aatggaaatc gtccttatta gggaagaagg agaaaagatc
     1381 ctacatgtca ggccaatagt ggaggaaggt aggagtttaa ttggcataat ccctgagtat
     1441 gaaagctctc ttaccttagc aataaagggt ggctttgaaa agacggctgc ctttatcagg
     1501 ttaatgtttc agtttgtagg aatgatattt agagggcagg tatcgaccca agacttggca
     1561 ggaccggtgg gagtaatcaa cgaggtgggc caggcggcga aaatgggttt taccagtcta
     1621 cttttaatat taggttttat tagtgtaaat cttggttttt tcaacctttt acctatacca
     1681 gccttagatg gaagtaggct agtatttatt tttatagagc taattagagg taagcccata
     1741 gatcctgaaa aggaaaattt catccatctt gtaggctttg catttctaat tggcttaatg
     1801 ttggttgtaa catatagaga tctaatcagg atcaatattt taaaataggt gatataggtg
     1861 aaaagaaaaa ctcgagtaat taaactgggg gaagtgccga taggtgggga taatccgatt
     1921 acaattcaat ctatgaccaa tacaattaca gcagatacag attctactat tgctcaaatt
     1981 aaggatttag aggcagccgg ctgtgatatt gtacgggtgg ccgtagccaa catagatgat
     2041 gctcaggcca ttaaagatat aaaggcacag attaatattc ctataatagc tgacatacaa
     2101 tacaattatc gtttggcatt agagtctatt aaatatggag tagatggatt aagaataaat
     2161 cctggtaata taggaagcct tacaagggtc aaggagatag ttaagtcttg taaggaaagg
     2221 agtattccta taaagatagg tgtaaattct ggatctatta aacaggaata tttggaaaaa
     2281 tataatgggg taaatgcaaa atccatggtc tatagtgccc tagagcagat aaagctttta
     2341 gaagaattag atttttatga tatcaaaatt tctttaaagg ccagtgacgt accccttact
     2401 atagaatcct atacaatgat ggctgaaatg agaaattacc cgctacatct agggattact
     2461 gaggcagggc ctgcttggcg gggtaccatc aaatcggctg tagggatagg aagtctccta
     2521 tctaaaggga taggagatac cataaggatt tccttaactg cagatccggt ggaagaaata
     2581 aaggttggaa gggagatatt aaaatcttta ggtttattaa accaagggtt agagattatt
     2641 tcatgcccta cctgcggtag aaccaggatt aatttaatgg aaatagttga agaagcagaa
     2701 aaaaggttgg aaaatataaa aggtcatatg aaggtggcta ttatgggttg tccagttaat
     2761 ggaccgggag aggctagaga agctgatata ggtatagcag gtggaaatgg taagggaata
     2821 atttttagta aaggccaaat agtaaaaaga gtaagagaag aggatttact agatgaatta
     2881 ataaaagagg taaaaaaaat tgataattta taagaagaaa taagggtgga gggatatcct
     2941 tatatgaaaa gaattaaatt ggctagtctt gttaaggatg aaaaagattc tcagctagga
     3001 gaattagata tagatattgt taaggtggaa gttgtacgag agttgatgga gataaactta
     3061 tttttagaag ctaaagaaat aatagatcta aggcaacagg aaaaaataga acaattctta
     3121 tcagaaaaat tccccttatt taaagtgaaa actaagatta agtattcact caataaagag
     3181 caattagaga aagttatggg ctcatatatt aaaaatcttg gactaattat tcaagaaggg
     3241 ataccatcag ccaattcctg gctagataaa ctagactata aagttaatga aggtacccta
     3301 actttaatac tgcccaatga attggccctg ttttctatga atcggaatgg attagttagt
     3361 ctacttaaga ataaggttca aacagagtta aaattgcaat tagagattat taaagatgat
     3421 aaaaaagaag tagaaaaagt tgacttttta gaggaccaga atatagagga aagaaagatt
     3481 tcaaaggatg ttttaaaaaa tacaaagaaa accagtagaa ataaaggaac ccaagctagg
     3541 accgatcttt tatacggtaa aaggataaag ggaagtccta taaaaataaa tgaggtaaac
     3601 ttgcagactg gcagtgcagt aataagaggt aaaatatttc agatagaatc taggagtatt
     3661 agagggaata ggagtctgat actcttcttt atttcagacg gtactgagtc tattcaggtg
     3721 aaactatttg tttcgaaaga agaaggattg aaactattat ctcaacttca ggaggatatg
     3781 tctttattaa ttgaaggcga tatagtttat gacaacttct ccaaaagtca ggtaatgatg
     3841 ttaaaatcag ccatggttgt taatgaagag attagaatgg ataaggctaa gaataagagg
     3901 gtagagttac acctccatac ccaaatgagc tctatggatg gggtagcaag tcttaaaaaa
     3961 ttggctgaaa gggcccacca atggggccac aaggccttgg ccataactga tcatggagtg
     4021 gtacaagcct ttcctgaggg aatggagctg agaaaaatta ggaataaaaa tgatctatgg
     4081 agttgaagga tatttagtaa atgatcagaa ggagataatc tcaaacttca atccagataa
     4141 ggaatatgaa gaatttattg tattcgatat agagactact ggtctatctg caattaatga
     4201 tgctattaca gaaattggag ctgtgaaaat aagagacggt caggtagttg atacctttgc
     4261 acaattaatt aaccctgaaa ggcctatacc agattttatt aaggagctaa ctggcattac
     4321 agatgacatg gtaagggata aacctaccat agaccaagta attcctaaat ttagtgaatt
     4381 tataggacag ggtatattag tagcccataa tgccagtttc gatgtaggtt ttattaggga
     4441 aaaaatgcga ttaaataata ttagtttaaa taatcctgta ttagatactc tggagctatc
     4501 tagaattgtt tttcctgaac taaaaagctt tagattaaat aacctagcaa aacatttggg
     4561 agttagcctg gaaaaccacc acagggccct agatgatgct atggcaacca aggatatatt
     4621 tataaagatc ttagaactgt tggaggaaag aaatataagc aatttaaagg atattaacca
     4681 gttaggtgct aaaggcaata taaataagga tagacccttt cacgtcacaa tattggctaa
     4741 aaaccaaaca ggattaaaga atttgtatag attaatttct gattcacata ttaaacattt
     4801 ttatagaagg cctaggatac ccaagtccct gttagaagag catagggaag gcttaatagt
     4861 tggaagtgcc tgcgaggcag gagagttg
//
LOCUS       DATGSI010000550         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_135034, whole genome shotgun sequence.
ACCESSION   DATGSI010000550 DATGSI010000000
VERSION     DATGSI010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135034"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VK031_08535"
     CDS             <1..1229
                     /locus_tag="VK031_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HLS54006.1"
                     /translation="ELTSLFNFNIPQRWSIAHLYQAILNNEEHIPKLSFFTTLAGYVH
                     WKLTGEKLLGIGDASGMFPIDEEIKDYDRGMIEKFNTLIEGRGVKLDIADLLPRCVLA
                     GEEAGVLTKEGARLLDPSGELEAGSIFAPPEGDAGTGMIATNSIATNTGNISAGTSVF
                     AMIVLEKQLSQVYPEIDMVMTPDGLPVAMVHANNCTSEINSWVGMFEDLLKRLNVEYD
                     INQLYQSLFLSALEADEDAEKYLVYGYHSGENITNIPEGRPLFARLPDAKLTVANFMR
                     AQLESAFATLNIGMQILFNEGIKLESIVGHGGIFKTEGVAQRVVAAAVESTVTVMETA
                     DEGGAWGMAILAEYRRSGKEIPLREYLHRYVFKDVKEIKYTPTQGEIDKYREYVKRYT
                     EGLDIERVAINSLKGN"
     gene            1273..1965
                     /locus_tag="VK031_08540"
     CDS             1273..1965
                     /locus_tag="VK031_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004839083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HLS54007.1"
                     /translation="MLKDLKKQVYEANMLLKESGLIVLTWGNVSGIDRDKGLFVIKPS
                     GVPYDELSPEDMIVMDLEGNKVEGDLNPSSDTPTHLELYRSFENIGGVAHTHSKWATI
                     WAQAGKGIEAYGTTHADHFYGMIPCTRDMTDEEIKGDYELETGKVIVETFKGLNPDHI
                     PSVLVKSHGPFSWGKDAMDAVTNAIVLEEVALMAYGTVQLKKDITKMDQNLLDKHFLR
                     KHGSDAYYGQNK"
     gene            2048..>2523
                     /locus_tag="VK031_08545"
     CDS             2048..>2523
                     /locus_tag="VK031_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54008.1"
                     /translation="MKISKKDFGTHNGQAVTEYTLDNGKNLVVKILNLGGVIREINFK
                     GKNRVLGFDNVEDYINKRGSIGALIGRVAGRISKGRMEIDGEVYELDKNEGTSCLHGG
                     EENFSKKLWTLVEEEVGEEQACISLKYKSADGEAGFPGELDVLVKYIIDKDDSLTM"
BASE COUNT          845 a          361 c          631 g          686 t
ORIGIN      
        1 aggagcttac tagcctattt aattttaata tacctcaaag atggagcatt gcccatttat
       61 atcaagcaat attaaataat gaggagcata ttccgaagct atcatttttt actacactgg
      121 caggatatgt acattggaaa ctaactgggg aaaaattatt gggaattgga gacgcctcag
      181 gcatgtttcc aattgatgaa gaaataaagg attatgacag gggaatgata gaaaagttta
      241 acactcttat tgaaggaagg ggtgttaagc tggatatagc tgaccttttg ccaaggtgtg
      301 ttttggccgg ggaagaggct ggagttttaa ctaaagaggg tgccaggtta ttggatccat
      361 ctggtgaatt ggaggcaggc agcatctttg cacctcctga aggggatgca ggaactggca
      421 tgatagccac caatagtatt gcaaccaata caggtaatat ttccgcagga acctcagtct
      481 ttgccatgat tgttctggaa aagcaattaa gccaggtcta tccggagatc gatatggtaa
      541 tgactcctga tgggctgcct gtagccatgg tgcatgccaa taactgtaca tcagagatta
      601 attcctgggt aggcatgttt gaagacttat taaaacgctt aaatgtagaa tatgacatta
      661 atcaactata ccaaagccta ttcttatcag ccctagaggc tgatgaggat gcagaaaagt
      721 atttggttta tggttatcat tctggagaaa atattacaaa tataccagag ggaaggccac
      781 tttttgctag gttgcctgat gccaagttaa cagtagctaa ttttatgagg gcacagctgg
      841 aaagtgcctt tgccacctta aatataggta tgcaaatcct atttaatgaa ggaatcaaac
      901 tagaatccat agtaggtcat ggtgggatat ttaagaccga gggcgtggct cagagagtcg
      961 tagctgccgc agtagaaagt acagtgacag ttatggaaac tgctgatgaa ggtggagcat
     1021 ggggaatggc catcttagca gagtatagaa gaagcggtaa agaaattcct ttaagggaat
     1081 atttacacag gtatgttttt aaagatgtaa aggagattaa atatacaccg acccagggtg
     1141 agattgataa atatagagaa tatgtaaagc gatataccga gggactggat atagaaagag
     1201 tagcaattaa tagccttaag ggaaattaac cctattatca gactttgtta gtttaatata
     1261 aggagttgta ttatgctaaa ggatttaaag aaacaagttt acgaggcaaa tatgctttta
     1321 aaggaaagcg gcttaatagt attaacctgg ggaaatgtct caggtataga tagggataag
     1381 ggactgtttg taataaaacc ttctggtgtt ccctatgatg aattaagtcc agaagatatg
     1441 atagtaatgg acttggaggg caataaggtg gagggtgact taaatccttc ttctgatact
     1501 ccaacccatt tagaattgta tagaagcttt gaaaacattg gtggagtagc ccatacccat
     1561 tccaagtggg caaccatatg ggcccaggcc ggtaaaggta tagaggccta tggaactacc
     1621 catgcagatc atttttatgg gatgattcct tgtactaggg atatgacaga tgaagaaatc
     1681 aaaggtgact atgaactgga gaccggtaag gttatagtgg aaacctttaa gggactaaat
     1741 ccagaccata tcccttccgt tttggttaaa tcccatggac cctttagctg gggtaaggat
     1801 gcaatggacg ctgtaaccaa tgctatagtt ttggaggaag tagccctgat ggcctatggt
     1861 acagtacagt taaagaagga tattacaaaa atggatcaaa atttattaga taagcatttc
     1921 ttaagaaaac acggtagcga tgcctactat gggcaaaata aataattgtt agatagaaaa
     1981 atagatttag gcattgccta aatctatttt taatcctatt tatagtataa aaagggggca
     2041 aatatatatg aagataagta agaaagattt tggaactcat aatggacagg cagttacaga
     2101 atatacgcta gataatggaa aaaatttagt agttaaaatt ttaaatctag gtggggtaat
     2161 tagggagatt aattttaagg gtaaaaatag ggtgttagga tttgacaatg tggaggatta
     2221 cataaataaa aggggctcta taggggcctt gataggcagg gtggctggta ggatttccaa
     2281 gggcagaatg gaaatagatg gggaagtgta tgaactggat aaaaatgaag gcaccagctg
     2341 tcttcatggt ggagaggaaa acttctcaaa gaagttgtgg accctggtgg aggaagaggt
     2401 tggggaagag caggcctgca tcagcttaaa atataaaagt gcagatggag aagctggttt
     2461 tccaggtgaa ttagatgtgt tggtgaaata cattatagat aaggatgaca gcttaaccat
     2521 gga
//
LOCUS       DATGSI010000551         1229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_509570, whole genome shotgun sequence.
ACCESSION   DATGSI010000551 DATGSI010000000
VERSION     DATGSI010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509570"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1021
                     /locus_tag="VK031_08550"
     CDS             245..1021
                     /locus_tag="VK031_08550"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS54009.1"
                     /translation="MSREKIVEIRNLGITYYTLKEETEALKDISLDIYDGEIISIVGP
                     SGCGKSTLLSIISGLIKPSYGQVKVNGKLVEGPLNNIGYMFQSDQLFEWRSILSNVCI
                     GLEIQNKLDQEAKTRAVDMLKTYGLGDFIYSYPYQLSGGMRQRAALIRTLLLEPDLLL
                     LDEPFSALDYQTRLAISDEIGLILKREKKTAIMVTHDIAEAISLSNRIVILSKRPGTI
                     KDIIDIKLTFTEDETPIKAREAPEFRHYFNRIWKELDIHV"
     gene            1014..>1229
                     /locus_tag="VK031_08555"
     CDS             1014..>1229
                     /locus_tag="VK031_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLS54010.1"
                     /translation="MSKIMSEKHKEFLKAVKSKNRTILATQIGLFLLVIIIWELAAQL
                     RLIDTFLTSSPSGIVRLLIEYTLRGSLF"
BASE COUNT          398 a          189 c          236 g          406 t
ORIGIN      
        1 ttgcttttta ttttgaaaat ttatcatata aatatacagg ctagatatgg ctataataag
       61 taaacatatg gctatggtta agtatatatt tgtctttttc cccttcatga ttatcccccc
      121 attaggaaaa gcctttcatc taccttgatt atacccaatc ttttacccta cccacttatt
      181 taatttcacc aaaggtatct attttgaata atatatctat atattccttt ttgggagtga
      241 taaaatgagt agggaaaaaa ttgtagaaat ccgtaattta ggtatcacct attatacttt
      301 aaaggaagaa actgaggcct taaaagatat aagtttagat atatatgatg gtgaaattat
      361 cagcattgta gggcctagcg gctgtggtaa atctaccctt ctttctatta tatctggcct
      421 gattaagcct agctatggtc aggttaaagt taatgggaaa ttagtggaag gtccacttaa
      481 taatatagga tatatgtttc aaagtgatca attatttgaa tggagaagca tcttaagcaa
      541 tgtttgtatt ggcctggaga tacaaaataa attggatcaa gaggccaaaa ctagagctgt
      601 agacatgcta aagacctatg ggctgggaga ttttatctat tcctatcctt atcaattgtc
      661 tggcggcatg aggcaaaggg ctgccttgat taggactctt ctattagagc ctgatctatt
      721 attgttagat gagcctttct ctgccttaga ctatcagact agactagcca tatcagatga
      781 gattggttta attttgaaaa gggagaaaaa aactgccatt atggtaaccc atgatattgc
      841 agaagccatt agtttatcta atagaattgt gattttaagc aaaagaccgg gaacaatcaa
      901 ggatattatt gatataaagt taactttcac cgaggatgaa acacctatta aggcaaggga
      961 agcacctgag tttagacact atttcaacag gatttggaag gagttggata tccatgtcta
     1021 aaattatgtc tgaaaaacat aaggaatttt taaaagctgt taagagtaaa aataggacca
     1081 tactggccac ccaaattggt cttttcctcc tggtaataat aatttgggaa ttggcggccc
     1141 agttaaggct tatagatact tttttaacca gcagtccctc tgggattgtt aggttgctaa
     1201 ttgaatatac tttaagaggt agtcttttt
//
LOCUS       DATGSI010000552         1443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_699895, whole genome shotgun sequence.
ACCESSION   DATGSI010000552 DATGSI010000000
VERSION     DATGSI010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699895"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VK031_08560"
     CDS             <1..314
                     /locus_tag="VK031_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54011.1"
                     /translation="AAKAILHDVQGENRKEIIEPSAVKINDDIFNKYKDKLAELGDRV
                     FQYVKVSEDLEAALEELNKLLKDEEIEKDIKAYQAVNAAKLIIKSSTKRKNSIGSFII
                     K"
     gene            422..613
                     /locus_tag="VK031_08565"
     CDS             422..613
                     /locus_tag="VK031_08565"
                     /inference="COORDINATES: protein motif:HMM:NF022223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor G inhibitor Gin"
                     /protein_id="HLS54012.1"
                     /translation="MYCNICGNMEKDINLFKITICKDCFYKLSNISVEDKEYERYKNL
                     VRIILSYYIGPRPKFYPVN"
     gene            complement(665..>1443)
                     /locus_tag="VK031_08570"
     CDS             complement(665..>1443)
                     /locus_tag="VK031_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLS54013.1"
                     /translation="SNSKFINVFINPVFSDNILIGAVLLFVDSTEKHKADLIRKEFSA
                     NVSHELKTPLTSINGYAEMIENGLVQGEDIKIFASIIKKEGDRLLKLIDSIIKLSKIE
                     NQVPNKDMDYVDLYSIAKVVSQRLNIFIKEKNLTFLLRGESTFVKANPTLIEELIYNL
                     LDNSIKYTETCGIITIDIYQELSKAIIKIKDSGVGIPIEHQDRIFERFYIVNKNRPHK
                     TKSTGLGLSIVKHIVQYHNASINLVSEPNKGTEIIIKFNL"
BASE COUNT          529 a          181 c          233 g          500 t
ORIGIN      
        1 tagcagctaa ggcaattctc catgatgtac agggtgaaaa tagaaaagaa ataatagaac
       61 cttctgctgt aaaaataaat gatgatatat ttaacaaata taaggacaag ttagcagaat
      121 taggggatag ggtcttccaa tatgtaaaag taagtgaaga tttagaagct gccttagaag
      181 aattaaataa gcttcttaaa gatgaggaga ttgaaaaaga tataaaggcc tatcaggcag
      241 tcaatgcagc taagttaatt attaaaagtt ctacaaagag aaagaattct ataggtagtt
      301 ttataataaa atagtaaagt tctttaataa taggaaaacc ctgccaagca caaaaaaaat
      361 tatttagaat aaaatatagt aggctggcaa taatctaaat aaaaggcagg agtgagtatt
      421 tatgtattgt aatatttgtg gcaatatgga aaaggatata aacctattta agataactat
      481 atgcaaggat tgtttctata aactttcaaa tatatcagtg gaagataaag aatacgaaag
      541 atataaaaat ttagtaagaa ttatacttag ttactacata ggccctagac ctaaatttta
      601 ccctgtaaat taatacaggg taaaatttag tattatatag tttccataaa gccttagaag
      661 agtatcaaag attaaattta attattattt cagtgccttt attaggctca cttactaggt
      721 taattgaggc attatgatat tggacaatat gcttgactat agaaaggcct aggccagtac
      781 ttttggtttt atggggtcta tttttattta caatatagaa tctttcaaaa attctatcct
      841 gatgctcaat aggtatacct actcctgaat ccttaatttt tataattgct ttagatagtt
      901 cttgatatat atctatggta attatgccac aggtttctgt atatttaata gaattgtcta
      961 aaagattata gatgagctct tctattagtg tagggtttgc cttaacaaag gtgctttctc
     1021 ctcttaaaag gaatgtaaga tttttttctt taatgaatat attaagcctt tgactaacta
     1081 ctttagcaat agagtatagg tcaacataat ccatgtcctt atttggaacc tgattttcta
     1141 ttttagatag ttttattata gagtcaataa gctttaataa tctgtctcct tcttttttaa
     1201 taatagaagc aaatatttta atatcttctc cttggactaa cccattttct atcatttcag
     1261 catatccatt tatagaggtc aagggagttt ttaattcatg agaaacatta gccgagaatt
     1321 cttttctaat aaggtccgcc ttatgttttt ctgttgaatc tacaaaaagt agaacagcac
     1381 ctataaggat attatcagaa aatacaggat ttatgaatac atttataaat ttagagttag
     1441 att
//
LOCUS       DATGSI010000553         1431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_135193, whole genome shotgun sequence.
ACCESSION   DATGSI010000553 DATGSI010000000
VERSION     DATGSI010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135193"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VK031_08575"
     CDS             <1..597
                     /locus_tag="VK031_08575"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HLS54014.1"
                     /translation="GGLDIKKIDQISENLLAAVFFLTVLAGLFSWVLTLFLPEQYSPI
                     SYLLPICLLGPFFYTLSETTAIGITLSRRTIYSMLASVGAMLVNALGNYLFVPKWGAL
                     GAASSTAIAFVIFYALRTEFSCLVWRQIPRKKQYNTILVLTAIAITFGFVAVQYEFTP
                     ALWAILLLLGTKHFRNQLIIFWKYINNQININGLGSGK"
     gene            587..>1431
                     /locus_tag="VK031_08580"
     CDS             587..>1431
                     /locus_tag="VK031_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54015.1"
                     /translation="MENKIQSFESINPYLVFLFFIFLSPFSNYITGSGKITYLPIIIA
                     GLYVVAYEIWSFKREDKKPIIEALLFLPFTILSLFAYYGHPFEEKALNYITFNLSLLP
                     VLALTAIRVFRYKQDSLEINIQTITNIIIAFAIGQLIICIGQFFTYTFGYGFPIVEEY
                     RHRNFISGTFYNPNNMSASILLLSFILLGLKKHLTIVQLRSSLIVFSLVLLFGGSRSA
                     IILFIAITIINLRPRPIKIAKYILLLFASIIFIYTLSTFVDNSALDRIIDRLHSFINI
                     LNRGIS"
BASE COUNT          429 a          291 c          231 g          480 t
ORIGIN      
        1 ggagggctgg atataaaaaa gattgatcaa atttcggaaa acctgctggc tgcagttttt
       61 tttctgaccg tattggcagg attattctca tgggtgctca ccttattttt gccagagcag
      121 tatagcccta tctcatattt attacctata tgcttactcg ggcctttttt ctatactcta
      181 tcggaaacaa cagccattgg catcacatta tccagacgca caatatattc catgctcgca
      241 tccgttggtg ccatgctggt taatgcactg ggtaattatc tatttgttcc caaatggggg
      301 gcattggggg ccgcctcctc tactgctatt gcttttgtca tcttttatgc gctacggaca
      361 gagttctcat gtttggtatg gcggcaaata ccaagaaaaa aacaatacaa cacaatcctt
      421 gtgctcacag caatcgcaat cacttttgga tttgtagcag ttcagtatga atttacacct
      481 gctttatggg cgatactgct actgcttgga acaaaacact tcagaaacca gcttattata
      541 ttctggaaat atataaacaa ccaaattaat atcaacggat taggcagtgg aaaataaaat
      601 tcaatcgttt gaaagtatta acccttatct ggtttttttg ttttttatat ttctgtctcc
      661 tttcagtaac tatattactg gatctgggaa aatcacctat ctgccaataa taattgcggg
      721 actgtatgta gtagcttatg aaatatggag ctttaagcga gaggacaaaa aaccaatcat
      781 agaagcatta ttatttctac cattcactat tttatccctc tttgcatact acggacaccc
      841 tttcgaagaa aaagccctga actatattac ctttaatcta tcactccttc ctgttctggc
      901 tcttaccgca attagggtat ttcgctataa acaggatagc ctggaaataa acatacaaac
      961 cataacaaac ataataatag catttgctat tggccagttg ataatttgca taggtcaatt
     1021 tttcacttat acattcggtt atgggttccc tatagtcgag gaatatcggc atagaaactt
     1081 catttcggga acattctata atcccaacaa tatgagcgcc tcaatcttgc tcttatcttt
     1141 tattctacta gggctaaaaa aacatctcac gatagtacaa ctaagatctt ctttaattgt
     1201 attttcttta gtcctgttat ttggaggttc aagatccgct attatattat ttatagcaat
     1261 aactataatt aatcttagac cacgtccaat caaaatagct aaatatattc tcctgctctt
     1321 tgcaagcatt atatttattt ataccctatc tacttttgta gataactctg ccttagacag
     1381 aatcattgat agactgcact cctttataaa catactgaat agaggaatat c
//
LOCUS       DATGSI010000554         1988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_558884, whole genome shotgun sequence.
ACCESSION   DATGSI010000554 DATGSI010000000
VERSION     DATGSI010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558884"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..568)
                     /gene="nifU"
                     /locus_tag="VK031_08585"
     CDS             complement(131..568)
                     /gene="nifU"
                     /locus_tag="VK031_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HLS54016.1"
                     /translation="MYSEKVMDHFMNPRNVGEITDADGIGEVGNAKCGDIMRMYLKIN
                     DGVIEDVKFKTFGCGSAIASSSMATELIKGKTIEEAMALTNRAVADALDGLPPIKMHC
                     SVLAEQAVKSALLDYSKRHNVDIPGLEDINFDEDHHEHNHIEE"
     gene            complement(569..1744)
                     /gene="nifS"
                     /locus_tag="VK031_08590"
     CDS             complement(569..1744)
                     /gene="nifS"
                     /locus_tag="VK031_08590"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HLS54017.1"
                     /translation="MKKVYMDNAATTPVKKEVLEEMLPYFTEKFGNPSSVYSLGSTSK
                     VAIEKAREQVAQVLGADKREIYFTGGGSESDNWAIKGAAFNNRKKGKHIITTKIEHQA
                     ILDTCKYLERQGFEVTYLNVDKDGLVDMEELKEAIREDTILISVMFANNEIGTIQPIK
                     EIGQIAKENDVIFHTDAVQAIGHIEIDVNELNIDLLSLSAHKFYGPKGVGALYIRRGV
                     RIDPLISGGGQESRKRGGTENVPAIVGMGKAIQLAYENFKEKNNYLIKLRDRLIEKIE
                     KNIDHVKLNGHRTKRLPGNVNISFEFIEGESLLLSLDMVGIAASSGSACTSGSLDPSH
                     VLMSIGLTHEIAHGSLRLSLGDFNTEDEVDYVVEQLIKIVERLRKMSPLYDNNKEEN"
     gene            complement(1773..>1988)
                     /locus_tag="VK031_08595"
     CDS             complement(1773..>1988)
                     /locus_tag="VK031_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HLS54018.1"
                     /translation="PEDITVGEVLRSLEGDLSPAECTSGKSIDCEKEENCATKLVLVK
                     IKDSIDEVIDSITLMDMIEDIKKMQRN"
BASE COUNT          557 a          441 c          262 g          728 t
ORIGIN      
        1 caggtagtaa attatcaaaa atagtaatat ggttagtagt atccacaaag cctttataaa
       61 aaaatctggt ggtccattta acagcaaatc atcaccccat atttaatgcc ttataaatat
      121 attataccat ttattcttct atatgattat gttcatgatg atcttcatca aaatttatat
      181 cttctaatcc tggaatgtcc acattatgtc ttttagaata atctaaaaga gctgacttaa
      241 ctgcctgctc agccaaaaca gaacagtgca tcttaatcgg tggtaagcca tctaaagcat
      301 ctgccactgc cctgtttgtt aaggccatgg cctcttctat ggtctttccc tttattaact
      361 ctgttgccat acttgatgaa gcaattgcag agccacaacc aaaggtttta aatttaacat
      421 cttctattac gccatcattt atttttaaat acattctcat aatgtctcca cacttagcat
      481 ttcccacttc accgatgcca tctgcatcag taatctcacc tacatttctg ggattcataa
      541 agtgatccat aaccttttct gaatacattt agttttcctc cttattattg tcatacaaag
      601 gtgacatttt tctaagtctt tctactatct ttatcagttg ttctaccaca taatctactt
      661 catcttctgt attaaagtct cccaggctta accttaagga accatgggct atttcatggg
      721 ttaaaccaat ggacattaaa acatgggacg gatccaagga accagaagta caggctgatc
      781 cactggatgc agcaattcct accatatcca agcttaatag tagggactca ccttctataa
      841 attcaaagga aatatttaca tttcctggta atcttttagt cctatgtcca tttaatttaa
      901 catgatctat gtttttctca atcttttcta ttaacctatc tctaagttta attaaataat
      961 tatttttttc cttaaaattc tcataggcca actggatagc ctttcccatt ccaactatgg
     1021 caggtacatt ttctgtacct cccctttttc tgctctcctg cccaccgcca gaaattaatg
     1081 ggtctattct tactcctcgt ctaatatata aggcccctac ccctttaggt ccataaaact
     1141 tatgggcaga tagggataaa agatctatat ttaattcatt tacatctatc tctatatggc
     1201 ctatggcctg aacagcatct gtatggaaga ttacatcatt ttctttggct atttgaccta
     1261 tttcttttat aggttgtatg gtgcctattt cattattggc aaacattaca ctaattaata
     1321 tggtgtcttc ccttatggct tcctttaatt cttccatatc aaccagccca tctttatcaa
     1381 cattcaaata tgtaacttca aacccttgtc tctctaaata cttacaagtg tctaatatgg
     1441 cctggtgttc aattttggta gttattatat gtttaccctt tttcctatta ttaaaagcag
     1501 ctccttttat tgcccagttg tcagattcag atccaccacc agtaaagtaa atctcccttt
     1561 tatctgcccc taagacctgg gcaacttgtt ccctagcttt ttcaatagcc accttggatg
     1621 tggaacctaa ggagtaaaca ctggatgggt taccaaattt ttcagtaaaa tagggtaaca
     1681 tttcttccaa aacttccttt ttaactggtg tagttgcagc attatccata tatacctttt
     1741 tcattttttc tcctcacctt ctgttttatc atttagtttc tttgcatttt cttaatatct
     1801 tcaatcatat ccatcaaagt tatagaatca attacttcat ctatactgtc tttgattttt
     1861 accagcacca gcttggttgc gcaattctcc tctttttcac agtcaattga tttaccagag
     1921 gtgcattctg caggtgataa atctccttct aaagatctaa gaacttcacc tacagttata
     1981 tcttccgg
//
LOCUS       DATGSI010000555         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_85316, whole genome shotgun sequence.
ACCESSION   DATGSI010000555 DATGSI010000000
VERSION     DATGSI010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85316"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VK031_08600"
     CDS             <1..656
                     /locus_tag="VK031_08600"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HLS54019.1"
                     /translation="EIAQTSSQGLLSIAAILGLWTSSSGLKAVIRAINRAYDYGETRS
                     FIKLRIISLLFTIALIVLLTLVSLTLVFGELLAKKIFLFFGGVHIFSTLWTYMRIIIP
                     LLYMILIFALLYKFSPCSNKRRKITLRSTLPGAVFTTLGWMITSIFFSYYVNNFGRFA
                     ITYGSLVGIILLLIWLYISSIIIVLGGEINATLEYFNIYGFHWNQNKSLVAKVINNN"
     gene            complement(658..1314)
                     /locus_tag="VK031_08605"
     CDS             complement(658..1314)
                     /locus_tag="VK031_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HLS54020.1"
                     /translation="MEYLEAFHNLIFPTRNLCYFCMERDYKVEKYLCPSCRGHLEVVN
                     KNITLNSYVDEAYYSILYNRMARKIIKDFKFNGKSYLFKPLGEFLLDTLNLIDIDYDK
                     VYFVPAHRRKEAIRGYNQSELLAKYLAERVNKPLSKGNLIKVKHTKDQSKLDRIGRMK
                     NLGGAFRIKNKRKVENKSILLIDDIITTGYTMMECGHVLKKAGAKKVVGLGVTSGQIL
                     "
     gene            complement(1324..>2202)
                     /locus_tag="VK031_08610"
     CDS             complement(1324..>2202)
                     /locus_tag="VK031_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HLS54021.1"
                     /translation="IDQLPSVGPGNVLRDIIDSESIEVVMLDEIFRQAEESMIIVNSH
                     RINKGQNPILNQKDKDFYFISKRNTRETLETIVNLNKERLPNFYKVDPIFDIQVLTPT
                     KRGDVGINSLNKHLQRALNPKSKERAEKQIGDEIFRVGDKVMQIKNNYNIEWKITKNN
                     MELDQGQGIFNGDFGYISHIDKEAQILEVLFDEEKKVRYDFNQLDELKLAYATTVHKA
                     QGSEFPVVIMPIHWGPPMLLTRNLIYTAITRARQLVVLVGEEKYLYMMIKNNRIAKRY
                     SALDKKIRNFLETFSL"
BASE COUNT          614 a          423 c          328 g          837 t
ORIGIN      
        1 cagaaattgc ccaaaccagt agccaagggc tgttatcaat tgcggctatc ttaggcttat
       61 ggacttcttc ttccggactt aaggcggtta tccgggccat aaatagggcc tatgattatg
      121 gtgaaaccag atcttttata aaacttagga taatttccct actttttact atagctttaa
      181 ttgttctatt aaccctggtt tctttgacct tggttttcgg tgaattattg gctaaaaaaa
      241 tattcttatt ttttggtgga gttcacatat tttccaccct ttggacatac atgaggataa
      301 tcatccccct tttatatatg atattaatat ttgctctatt atataaattt agtccttgca
      361 gcaataaaag gagaaaaatc accttaagaa gtaccctgcc aggggctgtt ttcaccacct
      421 tgggctggat gattacttcc atatttttct catattatgt taacaatttt ggacgttttg
      481 caataaccta tggtagtttg gtcggtatta tccttctgct aatatggcta tatatcagca
      541 gtatcattat agttttaggt ggtgagataa acgcaacctt agaatatttt aacatctatg
      601 gctttcactg gaatcaaaat aaaagtttgg tggctaaggt tatcaataat aattaattta
      661 aaggatttga ccagaggtca ctcctaaacc taccaccttt ttggctcctg ccttttttag
      721 tacatgtcca cattccatca tagtataacc tgttgtaata atgtcatcta ttaaaagaat
      781 acttttgttt tcaacttttc tcttgttttt tatcctaaag gccccaccca ggtttttcat
      841 tctacctatt ctatctaact tgctctgatc ttttgtatgt ttgaccttta taaggttacc
      901 tttagacagg ggcttgttta ccctttctgc caggtatttt gccaaaagtt ctgattgatt
      961 atatcccctt atggcctcct ttctcctatg ggcaggaaca aagtagacct tatcataatc
     1021 aatatcaatc aaatttaaag tatctaataa gaattcacct aaaggtttga ataggtaact
     1081 tttcccatta aatttaaaat cttttattat ttttctggcc attctattat ataaaataga
     1141 atagtaagcc tcatcgacat aagaatttag ggtaatgttt ttgtttacca cttcaaggtg
     1201 tcctctgcag gatgggcata aatatttctc tactttatag tctctttcca tgcaaaaata
     1261 gcagagattt ctagtgggaa atattagatt atgaaaggct tctagatact ccatatttct
     1321 cacctataga ctaaaagttt ctagaaaatt tctaatcttt ttatctaaag cagaatacct
     1381 tttggcaatt ctattatttt tgatcatcat atataggtat ttttcttcac ctactaaaac
     1441 aaccaattgc ctggccctgg taatggcagt ataaattaaa ttccttgtaa gtaacatggg
     1501 aggtccccag tggattggca ttatgaccac aggaaactca cttccttggg ccttgtggac
     1561 tgttgtggca taggccagct ttaattcatc cagctgattg aaatcatatc ttaccttttt
     1621 ttcttcatca aataatactt ctaagatttg tgcctccttg tcaatatggc ttatatatcc
     1681 aaagtcacca ttgaagattc cctgtccctg atctaattcc atattatttt tagtaatctt
     1741 ccactcaata ttatagttgt tttttatctg cattaccttg tctccaaccc taaaaatttc
     1801 atctcctatt tgtttttccg ccctttcctt ggacttagga tttagagccc tttgtaagtg
     1861 tttgtttaag gaatttatac caacatctcc acgttttgta ggcgttaata cctggatatc
     1921 aaagattgga tccaccttat aaaaatttgg taatctttcc ttgtttaggt taactattgt
     1981 ctctaaagtt tctctggtat ttcttttgga tataaaataa aaatccttat ctttttgatt
     2041 aagtattgga ttttgcccct tattaatcct atgggaattg actattatca tactttcttc
     2101 tgcctgccta aatatttcat ctagcataac cacttctata ctttccgagt caataatatc
     2161 tcttaaaaca tttcctggcc ctacactagg tagctgatct at
//
LOCUS       DATGSI010000556         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_228163, whole genome shotgun sequence.
ACCESSION   DATGSI010000556 DATGSI010000000
VERSION     DATGSI010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228163"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VK031_08615"
     CDS             <1..793
                     /locus_tag="VK031_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HLS54022.1"
                     /translation="FDFPVAIGFGLRDEEFFDQSVDFIKELKESSDKPFYTFMVTLTS
                     HTPFVIPEEYHELELEQELMDNMIGNYFQAIHYTDKHFGHFLERLKEEGLYDDTVIAL
                     YGDHFAIKADQEEATLLSDLLGMEYDYDEMMHIPLVIHVPGEDIKETISTVGSHLDFY
                     PTIANIMGLDNEKGLVFGRDLTNYDGETYVAPQTYLPKGSFISRDILFEMSKDGIFEH
                     SRAVNRITREEVDLEPLKDISEKLKLEIYKSTYILENDLLKDIID"
     gene            888..1220
                     /locus_tag="VK031_08620"
     CDS             888..1220
                     /locus_tag="VK031_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54023.1"
                     /translation="MQKNIPFKIIESDKKGLGKEAVTLAEDDDKVRLTITKEVQTPAY
                     SVEIEKIVEEDEYFKIYYRVVPPETDTVQLQVMTYKTIDLEINKEDIGDPPYKFVLNE
                     DKEKLIER"
     gene            1296..>2036
                     /locus_tag="VK031_08625"
     CDS             1296..>2036
                     /locus_tag="VK031_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HLS54024.1"
                     /translation="MGKENKVAQSAAIIAIFTLASKGLGFFREVLIASKFGSGMVTDT
                     YFVAMTATVIIMGTLGSALNTTLIPIFSEIRESSGRQGQRRYLNNILNLVFLITSLLV
                     IMAYILSPAIIRVLAKGFEGEQFKLAVRLNRIGLPIIIFLGFTHVFSGYLQSNEIFGP
                     HAIMGIPYNLVFLIYLIFSKGEKVELLMLISVFAALTQVLIQLPAVIHSRYRYSPRIN
                     LRDPYLYKTMSLVLPVLLGSAVHQINAIV"
BASE COUNT          720 a          310 c          395 g          611 t
ORIGIN      
        1 ctttgatttt ccagttgcta ttggatttgg tttaagagat gaggaatttt tcgaccaatc
       61 ggtagatttt ataaaagaat taaaggagtc cagtgacaag cctttttata cctttatggt
      121 aaccctaaca agccatactc cctttgtaat tcctgaagaa tatcatgaat tagaattgga
      181 gcaagaactt atggataata tgataggtaa ttattttcag gccatccatt atacagataa
      241 acattttgga cattttttag aaaggctaaa agaagaaggc ctctatgatg acacagtaat
      301 agccttgtat ggagaccatt ttgccattaa ggcagaccaa gaagaagcca ccctattatc
      361 agatttgcta ggtatggaat atgactatga tgaaatgatg catataccct tggtaattca
      421 tgtgccagga gaagatataa aagaaaccat atctactgta ggcagtcatt tggattttta
      481 tccaactata gcaaatataa tgggcttaga taacgaaaag gggctagtat ttggaaggga
      541 tttaaccaac tatgatggag aaacatatgt ggcaccacag acctatctac ctaagggctc
      601 atttattagt agggatatat tatttgaaat gtcaaaggat ggaatctttg agcatagcag
      661 ggctgtaaat agaataacta gagaagaggt agatcttgaa ccattgaagg atatatcaga
      721 aaaattaaag cttgaaattt ataaatccac ctatatatta gaaaatgatt tattaaagga
      781 tattatagat taatattttc taaaaattat ggtcttaatt aatattattt atataccagg
      841 cataggctat aataggacca tatccagatc ggattagggg ggagcttatg caaaaaaaca
      901 ttccatttaa aataatagaa tcagataaaa aaggcttggg aaaagaggca gtaactctgg
      961 cagaagatga cgacaaagta agacttacta ttacaaagga agttcagacc cctgcctatt
     1021 cggtggagat tgaaaaaata gtagaagagg atgaatattt taaaatatat tatagggtag
     1081 tccctccgga aactgatacg gtacaattac aggtaatgac ctataagact attgatttag
     1141 aaattaataa ggaagacata ggggacccgc catataagtt tgtattaaat gaggacaaag
     1201 aaaaactgat agaaagataa gggccaaagg attttcctct ctggcccttt tattatgata
     1261 taataaaaag acaatagact aaaagaggtt aaattatggg aaaagaaaac aaggttgctc
     1321 aatcggcagc tataatagca atatttacct tagccagtaa aggcttaggt ttttttagag
     1381 aggtattaat agcatctaaa tttggctctg gcatggtgac agacacttac tttgtggcca
     1441 tgacagccac tgtaattatt atgggtacac taggttccgc ccttaatact accttgattc
     1501 ctatattttc agaaataaga gaaagcagtg gaagacaagg gcagagaagg tatttaaata
     1561 atattttgaa tctggtattt ttaatcacca gcctactggt aataatggca tacatactat
     1621 ctccagctat aatcagggtc ctggctaaag gttttgaagg agaacaattt aagctggctg
     1681 ttaggctaaa taggatagga ctacctataa ttatattcct aggatttacc catgtattct
     1741 ctggatacct acagagtaat gaaattttcg ggccccatgc cataatgggt ataccctata
     1801 atctggtttt tcttatatat ttaatatttt ccaaagggga aaaagtagag ctattaatgc
     1861 ttataagtgt ttttgccgcc ttaacccagg ttttaattca actaccggca gtaattcact
     1921 ctagatacag atattctcct agaataaatt taagggatcc ttacctttac aaaaccatgt
     1981 ctctggtgct acctgtttta ttaggttcag ctgtacacca aatcaatgcc atagta
//
LOCUS       DATGSI010000557         1330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_367914, whole genome shotgun sequence.
ACCESSION   DATGSI010000557 DATGSI010000000
VERSION     DATGSI010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367914"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..902)
                     /locus_tag="VK031_08630"
     CDS             complement(186..902)
                     /locus_tag="VK031_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54025.1"
                     /translation="MPIQNEVITNKAHVYIYEDKAVKNFHDRSRNELEFANLKEVQAL
                     FREFKLNGWTYKFVQAFKVTKDEGIVMEKVLGVPMLDDPKFTADNYFHAGVWLGYFHK
                     STRQKDLVKSFGDFTFDNIFISHESKTITGMDPQMLKNKNYYLDLLLFFSSAIISGLK
                     RKLPYNTDCVNKFVEGYQTYSNFQYNREIYKNTVKKVLEGFKSGAVMKRHGKLRFYAG
                     HFLLGFNLKWHLKKVLKQYK"
     gene            complement(941..>1330)
                     /locus_tag="VK031_08635"
     CDS             complement(941..>1330)
                     /locus_tag="VK031_08635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLS54026.1"
                     /translation="YFEAHLALYDFISYKGIAESDKVHATLGEYDVLLLPTRHEGEGF
                     PGTILDAYLSGIPVIVSEWKFLPEFVDQNQTGFICKLGDNSGFYNKVIQLYENPSQLC
                     EMKRNAYEKSHEYSKDIAWDILKPYIA"
BASE COUNT          409 a          261 c          186 g          474 t
ORIGIN      
        1 tggaatttct aattggtcga agaaatctcc agacatcttt ttatcataat gctgtcctgt
       61 atgtaccaat ctaaaatcta tatccttccc ttcttctttg gccttattta tagcctgaat
      121 aataggagct attttcataa aattgggtct cgcaccagcg actatcgtaa tttgcatagt
      181 tgtatttatt tatattgttt taaaactttt tttagatgcc attttaaatt aaaaccaagt
      241 agaaagtgtc ctgcataaaa ccttagcttt ccatgtcttt tcataacagc accactctta
      301 aatccctcca ataccttttt gacagtattc ttataaattt ctctattata ctgaaaatta
      361 ctataagttt gatagccttc tacaaactta tttacacagt ctgtattata tggcagtttt
      421 ctcttaagac ctgaaataat agcactacta aaaaacaaga gcaaatcgag gtaatagttt
      481 ttattcttca gcatttgcgg atccattcct gtgatggttt tcgattcatg ggagataaaa
      541 atattatcaa aagtaaaatc cccaaagctt ttcactaaat ctttttgtct tgtagactta
      601 tgaaaatagc ctaaccaaac tcctgcatgg aagtagttgt ctgcagtaaa cttaggatcg
      661 tctaacatag gaacaccaag tactttctcc attacgatac cttcgtcttt agtgacttta
      721 aaagcttgta caaatttata tgtccatcca ttcaacttaa attccctaaa cagagcctgt
      781 acttctttta aatttgcaaa ctcaagttca tttctcgatc tatcgtgaaa gttctttact
      841 gccttatcct catatatgta tacatgagct ttattggtaa ttacttcgtt ctgtataggc
      901 atagtacatt atttctaaag ttataatatt tattcccacg ctaagctatg tacggcttaa
      961 gtatatccca agctatatct tttgaatact catggctttt ttcataagca ttgcgcttca
     1021 tttcgcacag ctgggaaggg ttctcatata attggataac tttattgtaa aaaccgctat
     1081 tatctcccaa tttacatata aatcccgttt ggttttggtc tacaaattca ggtaaaaact
     1141 tccattctga tacgattaca ggaatgccag acaggtaggc atctaaaata gttcctggaa
     1201 acccctcccc ttcatgtcgg gtaggtaata atagtacatc atactctcct aaggtagcat
     1261 gtaccttatc tgattcggca attcctttat aagagataaa atcatataaa gccagatgag
     1321 cttcaaaata
//
LOCUS       DATGSI010000558         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_700621, whole genome shotgun sequence.
ACCESSION   DATGSI010000558 DATGSI010000000
VERSION     DATGSI010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700621"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..2206
                     /locus_tag="VK031_08640"
                     /pseudo
     CDS             1..2206
                     /locus_tag="VK031_08640"
                     /inference="COORDINATES: protein motif:HMM:NF023295.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
BASE COUNT          933 a          304 c          534 g          637 t
ORIGIN      
        1 cttagatgac cagctttatg gtactttttc tagggtaaaa gattttttaa gacaggaacc
       61 agagcaggct agaagtaggg aacacctgaa agaactatta gatggaagga aggctaatgg
      121 gatctttttt actaccatgc aaaaattttc tgaatctgag gaaccactaa cagatagaag
      181 ggatattata gtgatttcgg atgaggccca taggagtcaa tatggtttaa gagaggttat
      241 taaaagggat ggttctatac aggtaggaat ggccaggatc attagagact cactgccaaa
      301 tgcatcttat atagggttta ccggtacgcc aatttctcag aaggacaagg atacacaaga
      361 agtttttggt gattatatag acatatatga tatgactcag gcggtagaag atggtgccac
      421 taaacccata tattatgaaa atcgtgttat gaatttaggc ttagatgaag atttattaaa
      481 agaaatagat gctaaatatg aagaattggc tacacaggca gctgaagaag acattgcaaa
      541 aagcaagaag gaattgggca aattagaatc aatattaggt acagattcag taatagatag
      601 cttatgcagg gatataatta accattatga gaatgaaagg gcccatctac ttaccggaaa
      661 ggccatgata gtggcctaca acagaaaaat tgctatgaaa atatatgaaa aaattttgga
      721 tgttaggcct gaatgggagg aaaaactcaa ggtagtcatg acaggtcgta ataatgaccc
      781 tgaggagtgg aaagatataa taggcaataa tcgatataga aaagaattag ccactagatt
      841 taaagacaac gaagatccat ttaaaattgc aatcatagtt gatatgtggt taacaggttt
      901 tgatgtacca agcctggcta ccatgtatgt attcaaacca atgaaggatc acaatttagt
      961 ccaggctatc gccagggtaa acagggtatt tgaggataag gaaggcgggc tagtagtaga
     1021 ttatattgga atagcaaagg ccttgaagaa tgcaatgaat gattacactg taagggatag
     1081 gaaaaacttc tccaatcctg atattaggga acatatttat cctaaatttc aagaaaagct
     1141 agaggtttgt cgtaatgaat ttttatatgg gctaaattat tcagaaatat ttagggatga
     1201 tgtggaggat agggtaaggt cggatctgat aacagaaggg attaaccata tatacaggtt
     1261 caatgaaaag caacagaaaa gcttcaagga agagtcctac atcctaagac aggctcatag
     1321 tttgtgttcc tctattacaa caagagctga gcaaagagag gcatcattta ttgaggctat
     1381 aaggattggt gtaaacagaa ttagggaaga aggccaaata tccttaaaag aaattaataa
     1441 gcagattaca gagcttttgg agcatagcat aaaaagtgaa ggaataatca acctattttc
     1501 agatgtggat acagagtttt caatattcaa tgaagaattt ttaaatagta tattagaaat
     1561 gaagcagaaa aacttagcta tataattgat gcacaaatta ctaagggaag atatacaact
     1621 cagggctaga acagatatag taaaatcgga ggaattctcc aaaagactga agaaagtcat
     1681 ggaaagttac agaagaagtc aaatagataa cgccaaaagc ctggatcttt tattacatga
     1741 atattataaa gatggtgaat ttactgaaga ggttatcagg gaaaaagagg atagttacgg
     1801 tatggatata gagagattta caagtcagga agattatact agggaactcc atgaaataat
     1861 caaagaccta ataaatatgg caagagatat ggtaagagcc gatcaggcag gagcagaaat
     1921 agggcttagc aaggaagagt tatcctttta taatgcgtta acctttactg aaggtattga
     1981 agatttttac gaggatgatg tattaataaa aatggcaaag gaattaacaa gacagctaaa
     2041 ggaaaatgaa tctatagact ggcagtacaa acaatcaggc agagcaagaa tgaggagtat
     2101 agtaaggaga cttttaacta aatataatta tccacctgag gatagaaagg aagccttaca
     2161 gatagtttta aaacaatgtg agcactggtc agaaacaagg atgtaattat aaattaatcc
     2221 aaaacaggtt aagaaatcag gccaggtaaa tattaaaatg ggcatattga aaagttatta
     2281 atactagagt aatttctagt agtattaaga cttcatcttg attgatcagt agttattgtc
     2341 ttaaaatact gatcgtctaa aggcgaagtc tttttaaatt taatttataa aatgagataa
     2401 ggaaattg
//
LOCUS       DATGSI010000559         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_510346, whole genome shotgun sequence.
ACCESSION   DATGSI010000559 DATGSI010000000
VERSION     DATGSI010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510346"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /gene="mltG"
                     /locus_tag="VK031_08645"
     CDS             <1..576
                     /gene="mltG"
                     /locus_tag="VK031_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HLS54027.1"
                     /translation="FEDEFEFLKELDQGQSLEGFLFPSTYEVYKDAKEEEIISRMLKQ
                     FEEVYNTDIKDRMADLDMTLNEIVTMASLVEREARVEEERPLIAGVYYKRLDIGMKLQ
                     CDATVQYALGERKERLLYKDLEIDSPYNTYLHYGLPIGPISSVGKASIQAALYPEETD
                     YLYYVARGDGSHIFTKTLEEHNKAKKEALGR"
     gene            609..1256
                     /locus_tag="VK031_08650"
     CDS             609..1256
                     /locus_tag="VK031_08650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HLS54028.1"
                     /translation="MSNINEKYIEKYIREVIPEKPIYLREMEDYAKKHHIPIIEPEVA
                     QFLKVILKVKKPRKILEIGTAIGYSALVMANALSGTCQITTIERREDMIALADQYIKK
                     TPYTDNIKIIKGQAEEILPRLKKKYDLIFIDAAKGQYMEFFNECTKCLNPEGLIISDN
                     VLFRGMVASDDLVVRRKKTIVKRLRQYLEYINEIPGYTSSLIPIGDGVALTYREA"
     gene            1261..>2036
                     /locus_tag="VK031_08655"
     CDS             1261..>2036
                     /locus_tag="VK031_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HLS54029.1"
                     /translation="MDKIELLAPAGDLEKLKMAITYGADAVYLGGEAFGLRAASKNFT
                     LDEIAEGVKYAHKRGKKVYVTLNIVPHDKDFEGLEEYVTNLYNLKVDAVIVSDPGMFS
                     VIKRTVPKLPIHISTQASVTNYETIMFWYNLGVRRIVLARELSLEEIRAITDRIPEDL
                     EIEAFVQGAMCMAYSGRCLLSNYMTGRDANMGDCAQPCRYKYHLVEQKRPGEYFPIYE
                     EDGGTFIFNSKDLCMLEHIPQLINSGITSFKIEGRVKSVY"
BASE COUNT          717 a          306 c          448 g          565 t
ORIGIN      
        1 tttgaagatg aatttgaatt tttaaaagag ttggatcagg gccaatcatt ggaaggcttt
       61 ttatttcctt caacctatga agtctataag gacgctaaag aggaagaaat tatatcaaga
      121 atgctgaaac aatttgagga ggtttataat acggacatta aagacagaat ggcagattta
      181 gacatgaccc taaatgaaat tgtaacaatg gcctccttag tggaaaggga ggcaagggtg
      241 gaggaagaga gacccttaat agccggtgta tactataaac gattagatat aggaatgaaa
      301 ttgcaatgcg atgccactgt tcaatatgct ttaggtgaaa gaaaggaaag gctactttat
      361 aaggatctgg aaatagattc tccatacaat acctatcttc attatggttt accaattgga
      421 cccatctcct cagttgggaa agcttctatc caggcggccc tttatccaga agagaccgat
      481 tatttatatt atgtggccag aggtgacggc agtcacattt tcactaaaac tttagaagaa
      541 cataacaagg caaaaaaaga ggccctaggc cgataacaaa actattctag caaataggga
      601 tgataaagtt gagcaatata aatgaaaaat atattgaaaa gtatataaga gaagttattc
      661 cagaaaaacc gatctattta agagaaatgg aggattatgc aaagaaacac catataccca
      721 taatagaacc tgaagttgcc cagtttttaa aggtaatctt aaaggtgaaa aagccaagga
      781 aaatattgga gataggtact gcaataggtt attcggccct ggttatggct aatgccttat
      841 ctggtacatg ccagataaca acaattgaac gtagggaaga tatgatagcc ctggcagatc
      901 aatatataaa aaagacaccc tatacggata acatcaagat tataaaagga caggctgagg
      961 aaatcttgcc tagactaaaa aagaagtatg atctaatttt tatagatgct gccaaggggc
     1021 agtatatgga gttttttaat gagtgtacca agtgccttaa tccagaaggc ttaattattt
     1081 ctgacaatgt tttgttcagg ggcatggttg cttcagatga tttggttgta cgcagaaaaa
     1141 aaaccatagt aaagaggctt agacaatatc ttgagtatat aaatgaaata ccaggctaca
     1201 cctcttcact gattcccata ggagacggtg ttgctttaac atatagagag gcgtgatatt
     1261 ttggacaaaa ttgaactttt ggcaccagcc ggtgatttag agaagttaaa aatggcaatt
     1321 acttacgggg ctgatgcagt gtatcttggt ggtgaggctt ttggccttag agctgcatct
     1381 aaaaatttta ccttagatga aatagcagaa ggggtaaaat atgcccacaa gagaggtaaa
     1441 aaagtatatg ttaccttaaa tatagtaccc catgacaaag attttgaagg cctagaggaa
     1501 tatgtgacca atttatataa cctaaaggtg gatgctgtaa tagtatcaga tccaggtatg
     1561 ttttctgtaa ttaaaagaac tgtaccaaaa ctgcctatcc acataagtac ccaggccagt
     1621 gttaccaact atgaaactat tatgttttgg tacaatttag gagttagaag gattgttttg
     1681 gccagggagt tatctttgga agaaataagg gcaattaccg atagaatacc agaggattta
     1741 gaaatagaag cctttgttca gggagccatg tgtatggcct attctggaag atgtttgttg
     1801 agtaattata tgaccggtag agatgctaat atgggggatt gtgctcaacc ttgcagatat
     1861 aaatatcatc tggtggagca aaaaaggcca ggagaatatt ttccaattta tgaggaagat
     1921 ggcggcacct ttatatttaa ctccaaggac ttatgtatgt tggaacatat tccccaactt
     1981 ataaattcag gaattaccag ttttaaaata gaaggtaggg taaaaagtgt ctacta
//
LOCUS       DATGSI010000560         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_461981, whole genome shotgun sequence.
ACCESSION   DATGSI010000560 DATGSI010000000
VERSION     DATGSI010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461981"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VK031_08660"
     CDS             <1..211
                     /locus_tag="VK031_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLS54030.1"
                     /translation="HGIIILQIHDKTPAQKAGIMANDIITKIDDIEVENMQTLKKSLY
                     KYRHGDKAVLTIVRNREEMEVEIIF"
     gene            387..1256
                     /locus_tag="VK031_08665"
     CDS             387..1256
                     /locus_tag="VK031_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HLS54031.1"
                     /translation="MKRLFSLLLILVLIFTMTACKKEEEIITMGFVPLIDGDKLIESV
                     EPLANMLTEEIGIKVEGFTATNYVGVVEGLGSGQVDFGIIPPFAYILANEESGAEVIL
                     SAINDQGKAKYRSEFLVRKDSDIKTFQDIKGKTVAFVDPSSTSGYLFPGAHLIKEGID
                     LEKDIDYMYSGGHDKSLQLLLNKDVDVIATFSHIRERYKEEFPTALEELEILGHTEDI
                     PFISVTTRKDMDPEIKEKLQDALLKLGETEEGRQLLPELFNIHGFIKATDEDYQVIRD
                     TAKIMDVDLEEIK"
     gene            1268..1999
                     /gene="phnC"
                     /locus_tag="VK031_08670"
     CDS             1268..1999
                     /gene="phnC"
                     /locus_tag="VK031_08670"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HLS54032.1"
                     /translation="MLKIEKLAVSYDGKKLAIDNINLKVGQGEFVGIIGASGSGKSTL
                     LKAINLLVRPRKGRVLIDNIDLTGLNSSQLRRVRRDIGFIFQDYNLIDRLTVLENVLI
                     GRLGYKSSFKSLLGLFTEEEYERASIALRQVGLKDKMFDRADQLSGGQMQRVAIAKTL
                     CQRPKIILADEPVASLDIASSQVVMDYFQRVNKKKNMTILINLHDVDLARKYCQRIIA
                     LKDGKVYYDGKAGDLDEKQLEKIYL"
     gene            1968..>2311
                     /locus_tag="VK031_08675"
     CDS             1968..>2311
                     /locus_tag="VK031_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HLS54033.1"
                     /translation="MKNSLKRYIYKRKLLSLLNLFIILIILIFLGYNVDFDPIRLYEG
                     MPSMFNLLKRMLRPNASYIGEVFEKLVETVEIAFISSLTGVFLALPLSLLVAENIAPS
                     IYLGSGLRAFFA"
BASE COUNT          797 a          347 c          508 g          659 t
ORIGIN      
        1 tcatggaatc ataattctac aaatccatga taaaacaccg gcccaaaaag caggtataat
       61 ggccaatgat ataattacca agattgatga tatagaagta gaaaatatgc aaacacttaa
      121 aaaatcccta tataaatata gacacggcga taaagcagtg ctaacaattg ttagaaatag
      181 ggaggaaatg gaagtagaaa ttatctttta aatattgtaa aagttgatgt aggccccagc
      241 ggtttccccc ttccgctggg gtttttactg ccctaggaac tgtacccata caaacaaata
      301 cacttatata aaaatgatga aagtgtattg aatcttttaa gaaagtgtag tagtctataa
      361 gtaagaaata tattaggggg attgcaatga aacgtttatt ttcactacta ctaatacttg
      421 tattaatttt tactatgacg gcttgtaaaa aagaagaaga aataattacc atgggctttg
      481 ttccattaat cgacggggat aagttaatag agtcagtgga gcccttggcc aatatgttaa
      541 ctgaggaaat aggaataaag gtagaaggct ttaccgcaac caactatgta ggtgtagttg
      601 aaggtttagg ctccggccaa gttgattttg gaataattcc gccttttgcc tatattttag
      661 ccaatgagga aagtggagca gaggttatat tatctgccat aaatgatcag ggaaaggcaa
      721 aatatagatc agaattcctg gtaaggaagg atagtgatat aaaaactttc caggatatta
      781 agggtaaaac agttgccttt gttgatccat cctctacttc aggctatctt tttccgggag
      841 cccatctgat aaaggaaggt attgatttgg aaaaagatat agattatatg tatagtggag
      901 gccatgataa atccttgcag ttactattaa ataaagatgt ggatgtaata gccactttct
      961 cccacataag ggaaaggtac aaggaagaat ttcccactgc cctagaagaa ttggaaattc
     1021 taggccatac agaagacatt ccttttataa gtgtaaccac aaggaaagat atggatccag
     1081 agataaagga aaagttacag gatgcccttt taaagctggg ggaaacggaa gaaggtagac
     1141 aacttctacc tgaattattt aatatacatg gatttataaa ggctacagat gaggactatc
     1201 aggtcataag ggatacagca aagattatgg acgtggatct agaagagatt aaataggggt
     1261 ggttccaatg ctaaagatag aaaagctggc agtatcctat gatggaaaaa aattagcaat
     1321 agacaatata aatctaaagg tggggcaggg agaatttgtg ggtattatag gggccagtgg
     1381 cagtggtaaa tccacccttt tgaaggccat caatttatta gtaagaccaa gaaagggaag
     1441 ggtcttaata gataatatag atttaactgg cttaaattct agtcagctta gaagggtaag
     1501 gcgggatata ggctttatct ttcaagacta taatctaata gacaggctaa cagttttgga
     1561 aaatgtccta ataggaaggc ttggttacaa gtcttccttt aagtcacttt taggcctatt
     1621 tactgaggaa gaatatgaaa gagctagcat tgccctaagg caggtaggcc ttaaggataa
     1681 aatgtttgat agggcagatc agttaagcgg tgggcagatg cagagggtgg ccattgctaa
     1741 aaccctttgc caaaggccta agataatttt agcagatgag ccggtggcta gcttggacat
     1801 agccagttct caagttgtta tggattattt tcaaagggta aataaaaaga agaatatgac
     1861 catattaatc aaccttcatg atgtggactt ggctaggaaa tactgccaaa gaattattgc
     1921 tctaaaagat ggcaaggtct actatgatgg caaggcaggt gacttagatg aaaaacagct
     1981 tgaaaagata tatttataaa aggaaactgc taagtctttt aaatttattt ataatattaa
     2041 taatcctaat ttttctaggt tacaatgtag actttgatcc tataaggctg tatgaaggca
     2101 tgcccagcat gtttaacctc cttaagcgaa tgctaagacc taatgcatct tatattggag
     2161 aagtttttga aaaattggtg gagaccgttg aaattgcctt catttcctcc cttacaggag
     2221 tatttttagc cctgcccttg tctctactag tggcagaaaa tattgcacct agtatatatt
     2281 taggaagtgg actgagggcc ttttttgcct t
//
LOCUS       DATGSI010000561         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_559704, whole genome shotgun sequence.
ACCESSION   DATGSI010000561 DATGSI010000000
VERSION     DATGSI010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559704"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VK031_08680"
     CDS             <1..134
                     /locus_tag="VK031_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HLS54034.1"
                     /translation="DGYQAGRWILLDFVDIIVHIFHREERDYYDLERLWTEDLGKVE"
     gene            152..532
                     /locus_tag="VK031_08685"
     CDS             152..532
                     /locus_tag="VK031_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLS54035.1"
                     /translation="MSLELLTTDKAPKAVGPYSQGVKAGNIIFTSGQLPIDPETGEMS
                     QADIQEQTRLCLENVKAVLEAAGASLEDVVKVTVFVTDMGQFGQINEIYGEYFSKHKP
                     ARSLVEVSKLPLGGQIEIEAVAIK"
     gene            complement(571..>1047)
                     /locus_tag="VK031_08690"
     CDS             complement(571..>1047)
                     /locus_tag="VK031_08690"
                     /inference="COORDINATES: protein motif:HMM:NF019555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54036.1"
                     /translation="GKEVYADTYKLLDYGYSNFETIALGREREFIDNVEIKNGTLPVV
                     SAILDRDILFTVKKDEQEEIKRKVVLNEDLKAPLSEGDLLGSVEYYLGDELIAEGNLV
                     STLSVGLLPPVKLYKRILDKWYIALLLVFIAYRCFHVFIRARKRRTSKYYGYTSMK"
BASE COUNT          349 a          199 c          196 g          303 t
ORIGIN      
        1 aagatggcta ccaggcaggt agatggattc ttttagattt tgttgatata atagtccata
       61 tttttcatag ggaagaaagg gactactatg acctagaaag attgtggaca gaagatttgg
      121 ggaaagttga ataacttaaa ggaggaattt tatgtcattg gaacttttaa caacagacaa
      181 ggcaccaaaa gcagtagggc cttattctca aggggttaaa gctggcaaca ttatctttac
      241 atcaggccaa ctacctattg atcctgaaac tggagaaatg tctcaagcag atatccagga
      301 acaaacaaga ctttgcctag aaaatgtaaa ggctgtattg gaagctgcag gggccagctt
      361 agaagatgtg gttaaggtaa ctgtttttgt aacagacatg ggccaatttg gtcagataaa
      421 tgaaatctac ggtgagtact ttagcaagca caagccagcc agaagtctgg tagaagtatc
      481 caagcttcca ctaggtggac aaatagaaat agaagcagta gcaattaaat aataatcaaa
      541 taagaaaaat aagctggttt ataaaccagc ttatttcatt gaagtatatc cataatattt
      601 acttgttctt cttttccttg ctcttataaa gacatggaag cacctatagg ctataaagac
      661 caataaaaga gctatatacc acttatccaa aatcctttta tatagcttga ctggaggaag
      721 caagcctacc gacaaggtgg agactagatt tccttcagct ataagctcat ctcccaggta
      781 atactctaca ctaccaagca ggtctccctc tgatagggga gcctttaagt cttcatttaa
      841 gactaccttt cttttaatct cttcctgttc atcctttttc acagtaaata gaatatccct
      901 atccaaaatg gcagacacaa caggcaaggt gccatttttt atttccacat tatctataaa
      961 ttccctttcc ctgcctaggg ctatggtttc aaaattacta tagccatagt ctaaaagctt
     1021 ataagtatct gcataaactt ccttacc
//
LOCUS       DATGSI010000562         1157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_36313, whole genome shotgun sequence.
ACCESSION   DATGSI010000562 DATGSI010000000
VERSION     DATGSI010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36313"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /gene="def"
                     /locus_tag="VK031_08695"
     CDS             complement(<1..444)
                     /gene="def"
                     /locus_tag="VK031_08695"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015777611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HLS54037.1"
                     /translation="MALRKIRTEGDPILRKKSRPIDEITDRIKTLLEDMEETMRDAQG
                     VGLAAPQVGILRRAVVIDVGDGIIKMINPEIIDTKGAEIDVEGCLSIPGIAGTVERPE
                     YVKVKFLNEDGEEVLLEGEGLLARAICHEIDHLDGILFTDKVIEYV"
     gene            complement(461..>1157)
                     /locus_tag="VK031_08700"
     CDS             complement(461..>1157)
                     /locus_tag="VK031_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HLS54038.1"
                     /translation="RMDSDTTSHRDIYEQIFADMKQEKIDILIGTQMISKGLDFKNVT
                     LVGIIAADTILNLPDFTAPERTFQLITQVAGRSGRGKHPGKVILQTYDPDHYSIRLAK
                     KQDYINFYEIEASLRKEFLYPPFINLIRILLYGEDQDKVGKISKKIYNIIGNRIYEIY
                     NNNYGNYINGPNPAPLEKIKNNYRWQVIVKVEDKYLDTMKELIEKVCIKNEYKIDMGG
                     IKLSIDINPNSIL"
BASE COUNT          317 a          265 c          145 g          430 t
ORIGIN      
        1 tacatattct ataaccttat cagtaaataa gataccgtct aaatgatcaa tttcatgaca
       61 gatggccctg gctaaaagac cctccccttc caacaggacc tcttccccgt cttcatttaa
      121 aaattttacc tttacatact ctggcctttc cacagtacca gctatgccag gtatagatag
      181 gcaaccttcc acatcaattt cagcaccctt tgtatctata atttctgggt taatcatttt
      241 tataattcca tcacccacat ctattactac tgcccttctc aatatgccta cctgtggagc
      301 tgctagacca acaccttgtg catctctcat ggtctcttcc atatcttcca gtaaagtttt
      361 tatcctatca gttatctcgt caattggcct tgacttcttt cttaagatag ggtctccttc
      421 tgtgcgaatt tttcttaatg ccatttactt tcctcccata ttacaaaata gaatttggat
      481 taatatctat acttaatttt ataccaccca tatctatctt atattcattt ttaatacata
      541 ctttctctat tagttccttc attgtgtcta gatatttgtc ttcaaccttc actataacct
      601 gccagcgata attattcttt atcttttcta aaggtgctgg atttgggcca tttatataat
      661 ttccataatt attattatag atttcataaa tcctattacc aattatatta tatatcttct
      721 ttgaaatttt gccaaccttg tcttgatcct ccccatacag taaaatccta attaaattta
      781 taaaaggtgg gtataaaaac tccttcctga gactagcctc gatctcataa aagtttatat
      841 agtcctgttt tttcgccaac cttatactat aatgatctgg atcataggtt tgtaaaatta
      901 ccttacctgg atgcttgccc ctacctgatc ttcctgccac ctgagtgatt aactgaaagg
      961 tcctttcagg tgccgtaaaa tctggcagat ttaggattgt atcagcggca ataataccta
     1021 caagggttac attcttaaaa tctaatcctt tggaaatcat ctgggttcct ataaggatat
     1081 caatcttttc ctgcttcatg tcagcaaata tttgctcata tatatctcta tggctagtgg
     1141 tatcactatc cattctg
//
LOCUS       DATGSI010000563         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_368479, whole genome shotgun sequence.
ACCESSION   DATGSI010000563 DATGSI010000000
VERSION     DATGSI010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368479"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VK031_08705"
     CDS             <1..254
                     /locus_tag="VK031_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54039.1"
                     /translation="GAYAAGSAKEKAILLIDGEMLFKEGELLADGEPHNPSNSERKQM
                     LIETLMHEFGHALEDLFKAEFDEDFIEQCVKSYRDYETK"
     gene            241..525
                     /locus_tag="VK031_08710"
     CDS             241..525
                     /locus_tag="VK031_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54040.1"
                     /translation="MKPSKYREQFEEEVGIALGLLDPVEDDYTLWLEEQLTERTNQVD
                     RLTSLLDNCKNAFLTLPEKALGECRDDTYKRWYIRDELIHNIKKVIKEVE"
     gene            522..830
                     /locus_tag="VK031_08715"
     CDS             522..830
                     /locus_tag="VK031_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54041.1"
                     /translation="MIEVLLASTDPEVISQFLEWAKVKHTLSFFLFVILLVVGGLMLS
                     DANKESDWEVMESQAHTFKVVFGILLVVVSVVKICVNIYYIAYIETAPELYLIEFFNE
                     "
     gene            836..908
                     /locus_tag="VK031_08720"
     tRNA            836..908
                     /locus_tag="VK031_08720"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Ile,seq:gat)
     gene            929..1198
                     /locus_tag="VK031_08725"
     CDS             929..1198
                     /locus_tag="VK031_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54042.1"
                     /translation="MNIEKELAQKLREVLFDCRSFFNQYRNLDGPNRKLKIQIKIEDT
                     IERLDMEISRSKRNSKYGNSNSTMGFNYSYKHSNRALNHYSRYKK"
BASE COUNT          597 a          236 c          332 g          470 t
ORIGIN      
        1 ttggggctta tgctgctgga tctgctaaag agaaagctat tcttctgata gatggagaga
       61 tgttgtttaa agaaggtgag ctattagcag atggagaacc acataatcct tctaatagtg
      121 agagaaagca gatgctgata gaaactctaa tgcatgagtt tggacatgct ttagaagatc
      181 tatttaaagc tgaatttgat gaggacttta tagaacaatg tgtaaaatct tatagggatt
      241 atgaaaccaa gtaaatatag agaacaattt gaggaagagg tgggtattgc acttggtcta
      301 cttgacccag ttgaggatga ttatactctt tggttagaag agcaactaac tgagagaact
      361 aatcaagtgg atagactcac ttcgctactt gataactgta agaacgcctt tctcactcta
      421 ccagaaaaag ctcttggtga gtgtagagat gatacttaca aaagatggta tattagggat
      481 gaacttattc ataacatcaa aaaggttata aaagaagttg aatgatagaa gtattacttg
      541 ctagtacaga tccagaagtg atcagtcaat tcctagaatg ggctaaagtc aaacatacct
      601 taagtttctt tttatttgtt atcctattag tagtgggggg attgatgcta agtgatgcta
      661 ataaagaaag cgattgggag gtaatggaaa gtcaagccca tacatttaag gtagtctttg
      721 gtatactctt agtagtagtg tctgtcgtta agatatgtgt taatatatac tacatagctt
      781 atatagagac agctcctgag ctatatctaa tagagttttt taacgagtaa acacaaggct
      841 tatagcttaa ttgggtaaag caccacactg ataaagtgga gatgtaggtt cgagtcctac
      901 taagcctttt acctaaaata gaggacatat gaacatagaa aaagagctag cccagaagtt
      961 aagagaagtc ttatttgatt gtagaagctt cttcaatcaa tatagaaacc ttgatggacc
     1021 taacagaaag ctcaagattc agataaagat agaggacact atagaaagat tagatatgga
     1081 aatatctagg tctaaacgca attcaaagta tggaaacagc aattctacta tgggctttaa
     1141 ttatagttat aagcatagca atagggctct taatcactat agccgctata agaaataagg
     1201 tgttctacct acattctaag acatacctta ataaagaaga ggtgatgtac atattaaacc
     1261 tatcccatga taagtacatc agggattatt ctaactggtt tgataagaac atcaatagac
     1321 atatatagaa gcattagtca atagaggaga taactccatg aaggagttgt ttacagagag
     1381 agatacctag tcaacatatg atactaacta ggttgcacat acaaaccata tagttagtat
     1441 tgctaaacat ttaaaaactc tctaaacaaa taagttatga gtaacaataa agaccaaagt
     1501 gtaaaacagt cagctgaaga tatcacaaaa gagaactttg tctggaggac taaagacaaa
     1561 gaagtaactc ttgatgagat ggatattgac ttcaagttgg ctgctcttat acactctttt
     1621 aagctatcca atact
//
LOCUS       DATGSI010000564         1407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_368503, whole genome shotgun sequence.
ACCESSION   DATGSI010000564 DATGSI010000000
VERSION     DATGSI010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368503"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /gene="proS"
                     /locus_tag="VK031_08730"
     CDS             complement(<1..670)
                     /gene="proS"
                     /locus_tag="VK031_08730"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HLS54043.1"
                     /translation="MRMSKMYMPTLKETPAEAEIDSHKLLLRAGMIRRLVSGVYSYLP
                     LGYRVIRKVEQIVREEMDKAGSQELLMSAIQPRELWEATGRWDNFGPEMWKLKDRHGR
                     EFCLGPTHEEYFTDLIKNEINSYKQLPLNLYQIQTKYRDEKRPRFGLMRSREFIMKDA
                     YTFDVDEKGMVESYNLMWQAYDNVFTRLKLEYKVVEGDTGAMGGNVSHEFMATSDSGE
                     GLIAF"
     misc_binding    complement(821..1029)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1041..>1407)
                     /gene="ispF"
                     /locus_tag="VK031_08735"
     CDS             complement(1041..>1407)
                     /gene="ispF"
                     /locus_tag="VK031_08735"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HLS54044.1"
                     /translation="AIMDGILGALALGDIGRHFPDNDPKYKDISSLELLKRVYELMVD
                     KGYEIGNIDATVAAEQPKLGPYIKDMKKIISNILNTGIGNINIKATTTEELGPIGQGE
                     GISAYAVCLLKTIDFNPAI"
BASE COUNT          388 a          343 c          198 g          478 t
ORIGIN      
        1 agaaggcgat taacccttcc cccgaatctg aggtggccat aaattcatgg gacacatttc
       61 cacccatagc tcctgtgtca ccttccacca ccttgtattc taatttaagt ctggtaaata
      121 cattgtcata ggcctgccac attaaattgt atgactctac cattcccttc tcatcaacat
      181 caaaggtata ggcatccttc ataataaact ctctacttct cataagccca aatcttggcc
      241 ttttttcatc cctgtatttt gtttgtatct ggtaaagatt taggggtaat tgtttatagg
      301 aatttatctc atttttaatc aaatctgtaa agtattcctc atgggttggc cctaagcaaa
      361 attccctgcc atgcctatct ttcagcttcc acatctcagg accaaaatta tcccatctac
      421 ctgtggcctc ccaaagttct cttggttgga tggcagacat taaaagctct tgggagccgg
      481 ccttatccat ttcttccctt actatctgct ccacttttct tataactcta taacctaatg
      541 gcagataaga atatacacca ctaactagcc ttcttatcat tcctgccctt agcaaaagct
      601 tatgactgtc tatttctgcc tccgctggtg tttcttttag tgtgggcata tacatttttg
      661 acattctcat cattaaccac tccttttatt gtaaatatat agatacctct agggtaaaaa
      721 ctgtctaatt aattataagt tccttgattg gtttctttat taaatattca gacgatacta
      781 cttacaactt aaaagaacaa tttaaaaaac ccttcatccc tgtagagacg aaaggttact
      841 tccgcggtac cactctaata aaccataagg ttcccttaat agctataacg taactaccgt
      901 ctagatttac taataccttc catctagatg ctccagggct ggttccttgc caagagggta
      961 aaggccttcc accaaatagc cttctctctg caaccattaa caagtactat tccctttccc
     1021 agccatcact atcaatatta ctaaattgcg gggttaaagt caattgtttt caacagacac
     1081 acagcatagg ccgatattcc ttccccttgt ccaataggtc ccaattcttc tgttgtggta
     1141 gcctttatgt ttatattacc aataccagta tttaatatgt ttgatattat ctttttcata
     1201 tcctttatat atggacctaa tttgggctgc tcagcagcca cagttgcgtc aatatttcct
     1261 atttcataac ccttatctac cataagttca taaactctct taagtagttc caagcttgaa
     1321 atgtccttat acttaggatc attatctgga aagtgtctac ctatatctcc cagtgccaag
     1381 gctcctaaaa tgccatccat aattgca
//
LOCUS       DATGSI010000565         1676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_181656, whole genome shotgun sequence.
ACCESSION   DATGSI010000565 DATGSI010000000
VERSION     DATGSI010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181656"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /gene="purH"
                     /locus_tag="VK031_08740"
     CDS             complement(<1..291)
                     /gene="purH"
                     /locus_tag="VK031_08740"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585998.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HLS54045.1"
                     /translation="MVRRALISVYNKEGIIDLALSLERLNWELISTGGTAQLLKEAGI
                     KVTEVEDVTSFPEILDGRVKTLNPYIHGGLLYKRDKKDHVATLEKLKIFPIDM"
     gene            complement(294..917)
                     /gene="purN"
                     /locus_tag="VK031_08745"
     CDS             complement(294..917)
                     /gene="purN"
                     /locus_tag="VK031_08745"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HLS54046.1"
                     /translation="MVKIGVLISGGGSNLQALIDKVKVGEIKGDIRLIISNNKGAYGI
                     ARGRKAGIESIYIDPKEFENMEEYNRRLIEEFKKRKIDLIVLAGFLKILSKEFISTFR
                     NRIINIHPSLIPSFCGKGHYGERVHRKVLDYGCKITGATVHFVDEGTDTGPIILQEAV
                     RVEEGDTVESLGKKVLKVEHRLLPEAVKLFCDNRIVLRGRKTIIKQE"
     gene            complement(911..>1676)
                     /gene="purM"
                     /locus_tag="VK031_08750"
     CDS             complement(911..>1676)
                     /gene="purM"
                     /locus_tag="VK031_08750"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HLS54047.1"
                     /translation="VNDILCQGAKPLFFLDYLATGRLVPEKMAQVVEEISNACIKSGV
                     VLIGGETAEMPDFYKSDEYDMAGFAVGLVDRKKIIDGSQIEEGDHIIGLPSTGLHSNG
                     YSLVRKIVFEKMGLKVDSYIEELASTIGEELLKPTRIYTEPVYDLIEKFNLKGISHIT
                     GGGFYENLPRIMPKGLTANIDRKKIEKHKIFDLLQVWGNISTKEMYSTFNMGVGMVLV
                     VAKAEIDQVKKSLEEREEKYYILGQVSRGKKGVALW"
BASE COUNT          440 a          409 c          220 g          607 t
ORIGIN      
        1 catatctatg ggaaatattt ttaatttttc aagtgtggcc acatgatcct ttttatccct
       61 tttatataat agaccgccgt gtatatatgg atttaaggtc tttaccctgc catctaaaat
      121 ttctggaaaa ctggttacat cttccacttc tgtaaccttt atgccagcct cttttagcaa
      181 ctgggctgtt ccccctgtag aaataagctc ccagtttaat ctttctaaac ttagagccag
      241 atctataatg ccttccttat tataaacgct gattaatgcc cttcgcacca caatcactcc
      301 tgtttaatta tagtttttct tccccttaaa acaatcctat tgtcacaaaa aagctttact
      361 gcctcaggta aaagcctgtg ctctactttt agcacctttt tccccaaact ttcaacagta
      421 tctccctctt ctacccttac ggcctcctgt aagataatgg gacctgtatc tgttccctca
      481 tctacaaaat gaacagtagc tccagtaatt ttacatccat agtcaagaac tttcctatgg
      541 actctttctc cataatggcc cttgccacaa aaactaggaa tcagggaagg atgaatattg
      601 attattctat ttctaaaggt agatatgaat tccttggaca aaatctttaa aaagccagcc
      661 aatactatta aatctatctt tctttttttg aattcttcta ttagccttct attgtattct
      721 tccatatttt caaattcttt aggatctata tatatacttt caatcccagc ctttcttccc
      781 ctggcaatcc cataggcccc tttattgttt gagattataa gcctgatgtc tccctttatt
      841 tctccgacct ttaccttatc tatcaaggcc tgtaagttac tacctccacc tgagattaaa
      901 actcctatct ttaccataag gctactccct tcttgcccct acttacctgg cctaggatat
      961 agtatttttc ttctctttcc tctaagctct ttttaacctg gtctatttca gctttggcta
     1021 ctactagaac catccctaca cccatattaa aggtagaata catctccttt gtagatatat
     1081 tgccccatac ttgtaacaga tcaaatattt tatgcttctc tattttcttc ctgtctatat
     1141 tggctgttaa tccttttggc attatcctag gtaagttctc ataaaatcct ccccctgtta
     1201 tatgggagat ccctttaaga ttaaattttt caattaagtc atatacaggt tcagtatata
     1261 ttctagttgg ttttaacaat tcttcaccta tggtgctagc taactcttct atatagctat
     1321 ctactttaag gcccattttc tcaaaaacaa tttttctaac caaggaatag ccattactgt
     1381 ggagccctgt ggaaggcagc cctattatat ggtctccttc ttctatttgg gagccatcga
     1441 taattttctt cctatctacc aatcctactg caaagcctgc catatcatat tcatcacttt
     1501 tgtaaaagtc tggcatctct gctgtttcgc cacctattag tactactccc gacttgatac
     1561 aagcattgga tatctcttct accacctggg ccattttttc aggtactaac cttcctgtgg
     1621 ccaggtaatc taagaaaaat aagggcttag ctccctggca caaaatatca tttacg
//
LOCUS       DATGSI010000566         1234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_510684, whole genome shotgun sequence.
ACCESSION   DATGSI010000566 DATGSI010000000
VERSION     DATGSI010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510684"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..>1234
                     /locus_tag="VK031_08755"
     CDS             6..>1234
                     /locus_tag="VK031_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HLS54048.1"
                     /translation="MNKILEAILSDIKNLIKIDNPKKFILSNIPYLSFFYIGNIFSKH
                     INSYVGEDIIDRIMVGISDIGTLSYIPSINPRDLLVGVSVAGLVKLIVYSKGKNKKKY
                     RQGKEYGSARWGESKDIVPYIDPKFENNVLITNTERLTMNSRPKNPKYARNKNVLVIG
                     GSGSGKTRFYVKPNLMQMHSSYVVTDPKGTLVLECGKMLYENGYDIKILNTINFKKSM
                     KYNPFAYLRSEKDILKLVQTIIANTKGDGEKAGEDFWVKAEKLYYTALIGYIYYEAPE
                     EEKNFKTLLDMIDASEVREDDETYMNPIDRLFEALEKKDPTHFAVKQYKKYKLAAGKT
                     AKSILISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISDTDDTFNFVVSI
                     MYSQLFNLLCDKADDVYG"
BASE COUNT          472 a          168 c          223 g          371 t
ORIGIN      
        1 ctacgatgaa taagatatta gaagccattc tttctgatat taaaaactta attaaaatag
       61 acaacccaaa gaaatttata ttatcaaaca ttccttatct atcatttttc tacattggaa
      121 atatcttttc taagcacatc aattcttatg taggagaaga tattattgat agaataatgg
      181 tgggaatttc tgatatagga actttatctt atataccaag tattaatcca agggacttgt
      241 tagtaggtgt ttcagttgct ggtcttgtta agctaattgt ttatagtaaa ggtaaaaaca
      301 aaaagaaata taggcaaggt aaagagtatg gatctgcaag atggggagaa agtaaggata
      361 ttgttccata tatagaccct aagtttgaaa acaatgttct cataactaat accgaaagac
      421 taacaatgaa ctcaagacct aaaaacccta aatatgctag aaataaaaat gtattggtaa
      481 taggtggttc tggatcaggt aaaacaagat tttatgtaaa gccaaaccta atgcaaatgc
      541 actcttccta tgtagtaaca gaccctaaag gcacacttgt gttagagtgt ggaaaaatgc
      601 tttatgaaaa tggatatgac ataaagattt taaatacaat aaactttaaa aaatctatga
      661 aatacaatcc ctttgcttat ttaagaagtg aaaaagatat tttaaaactt gtccaaacta
      721 taattgctaa caccaaggga gatggagaaa aggcaggaga agatttctgg gtaaaggctg
      781 aaaagctcta ttacactgcc cttatcggtt atatttatta tgaagcacct gaagaagaaa
      841 agaactttaa gacacttttg gatatgattg atgcaagtga agttagagaa gatgacgaaa
      901 cctatatgaa cccaattgat aggctctttg aagctcttga aaagaaagac cctactcatt
      961 ttgcagttaa gcaatacaag aaatataagc ttgcagcagg aaaaactgct aagtcaattc
     1021 taatatcttg tggagcaaga cttgcacctt ttgatataag agagcttaga gaactaatga
     1081 gtgaagatga acttgaactt gataaaattg gagataggaa aactgctcta ttcgtaataa
     1141 tatcagatac agatgatacc tttaactttg tagtttcaat aatgtattct cagttattta
     1201 atctactatg tgataaggca gatgatgtgt atgg
//
LOCUS       DATGSI010000567         1059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_701163, whole genome shotgun sequence.
ACCESSION   DATGSI010000567 DATGSI010000000
VERSION     DATGSI010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701163"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VK031_08760"
     CDS             complement(<1..873)
                     /locus_tag="VK031_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HLS54049.1"
                     /translation="MSLNNLTFKGGIRIKGEKELTKDKPIEKALEPEMVHIPLHQHSG
                     APCKPLVKRGDKVKVGQKIGESEAFLSAPVHSSVSGVVRRIGNTYTHDGYNVQCVVIE
                     SDGLNEIHESVKPKGDLESLSKEEILDAIREAGIAGMGGAAYPTHAKIADCVEDGVQT
                     VILNGAECEPYLTCDYRIMVEKPDLVVEGLRAILKIVDSNDGYIGIEDDKMEAVEKIK
                     EAAAKENNIKVATMKTKFPQGDSYRLVDSLTGRKVPKGGRCKDVGAIVNNVGTAVAIA
                     EAILEGKPLYERVVT"
BASE COUNT          271 a          244 c          164 g          380 t
ORIGIN      
        1 agttacaacc ctttcataca aaggctttcc ttccagtatg gcctctgcaa ttgcaacagc
       61 tgtacctaca ttatttacta tagctcctac gtctttacat ctgccacctt taggtacctt
      121 tctgcctgta aggctgtcta ccaatctata ggaatcccct tgaggaaatt tagttttcat
      181 agtagctact ttaatgttgt tttccttggc tgcagcttcc ttaatctttt ctacagcttc
      241 catcttgtcg tcttcaattc ctatgtaacc gtcattgcta tcaactatct ttaagatagc
      301 ccttaaacct tcaactacca gatccggttt ttccaccata attctataat cacaggttag
      361 atatggttca cactctgctc catttaggat aacagtctgt actccatctt ctacacagtc
      421 tgctattttc gcatgtgttg gataagctgc tcctcccatg ccggcaatac ctgcttccct
      481 tatggcatct aggatttctt ccttacttag agactctagg tcccctttag gttttactga
      541 ttcgtggatc tcgtttaatc catcagactc tattacaaca cattgaacat tgtatccatc
      601 atgggtatag gtgttgccta ttcttctaac cactcctgat acacttgaat ggactggtgc
      661 tgataagaag gcttcagact ctccaatttt ctggcctacc tttaccttgt ctcccctttt
      721 tactaaaggt ttacatggag cacctgagtg ctggtgtaaa ggaatatgta ccatttctgg
      781 ttctagggcc ttttcaatgg gcttgtcttt tgttaattcc ttttcgcctt ttatacgaat
      841 tccaccttta aaagtgagat tattaagact cattaattat tccacctctt tgctctattt
      901 taatttaaat tgaaagttaa gttttgtctc ttaacccaat tccaaagctc attataacat
      961 taattgttaa aaatgtacac aagttagtta gaaattaaac atattttgca aaaacaaaag
     1021 atagtctatg taactggtgc agaggaaggg agtcgaacc
//
LOCUS       DATGSI010000568         1932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_319634, whole genome shotgun sequence.
ACCESSION   DATGSI010000568 DATGSI010000000
VERSION     DATGSI010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319634"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VK031_08765"
     CDS             complement(<1..543)
                     /locus_tag="VK031_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HLS54050.1"
                     /translation="MMKIKVNVVGLGEVQVDKGTSLLQLSKEIYKDQYKKYLGARINN
                     QIYNLNKTIEEEIEIRFLDNRDVDGYRIYTKTISAVFIMACKELYPEATVKINHFIGP
                     GLYVELDTEEGIRFTQLQEVEKKMKEIVEKDYDIVRTRHPKEEALKTFEKHGYTDKIR
                     LFSSIDKKEVSIYKINDHIDS"
     gene            complement(650..1555)
                     /locus_tag="VK031_08770"
     CDS             complement(650..1555)
                     /locus_tag="VK031_08770"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HLS54051.1"
                     /translation="MKFAKIGMRTVKTVIAVILTLIVAEVFKLRSPLLASIAAIMTME
                     SSISESFVTGTNRMYGTILGGFTALIVTLIVPGNFIALAIGLIFIINICTKFNWEKAA
                     RISMVVFLAIVLNYDMDDGLSYAVNRTLDTLIGVIIGTAINYFVRPPKLDEKIKQTAE
                     KMLRTTQTMLERLIWKQEYDNLDHFSNEIDSMKKDYKTLREDLKFQIGKTTNTAIYAD
                     LSEAFEKLQLHMSILHLIKEWPVINKNNKDTIEGYYGKAIPKEKNGNDDELDVIYNYH
                     LESLMKELLSIKELIYKSQDIVESN"
     gene            complement(1735..>1932)
                     /locus_tag="VK031_08775"
     CDS             complement(1735..>1932)
                     /locus_tag="VK031_08775"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HLS54052.1"
                     /translation="TLMPREQSILEMRYGLNGYEPHTLEEIGLKYDLTRERIRQIQER
                     ALRKLRHPSKGKYLEEFLYKK"
BASE COUNT          558 a          376 c          231 g          767 t
ORIGIN      
        1 ggaatctata tgatcgttaa ttttgtatat gctaacttct tttttatcta tgctgctaaa
       61 tagccttatt ttgtctgtat atccatgttt ttcgaaagtc tttaaggcct cttccttagg
      121 gtgtctagtt cttacaatgt cataatcttt ttctactatt tccttcatct ttttctcaac
      181 ttcttgtagc tgggtaaatc taattccctc ttctgtatct aattctacat acaaacctgg
      241 ccctataaag tgattaatct ttacagttgc ttcaggatat agctccttac atgccataat
      301 aaaaacagcc gaaatagttt ttgtatagat cctatatccg tccacatccc gattatctaa
      361 aaatcttatt tctatttcct cttctatagt tttatttaga ttgtaaattt gattattaat
      421 tctagctcct aggtatttct tatactggtc cttgtaaatc tctttagata gctgaagtaa
      481 acttgttcct ttatctactt gaacttctcc tagacctaca acatttacct taatcttcat
      541 caaagatcaa tccccttcta ataatgtgtt atataatatg gtatcacaaa cttaatactt
      601 tgcataaagc tatgtcccat ttcttttctt ttaataccta cttggtaagt taattgcttt
      661 ctacaatatc ctgactcttg taaattaatt cctttataga taataactcc ttcatcaggc
      721 tttccaaatg atagttgtag attacatcta actcatcatc attaccattt ttctccttgg
      781 gaatagcctt gccatagtaa ccttctatgg tgtccttatt gtttttatta attacaggcc
      841 attctttaat taaatgtaat atactcatat gaagttgtag tttttcaaag gcctccgata
      901 aatctgcata tatggctgta tttgtagtct tccctatttg gaattttaaa tcttctctta
      961 gagttttata atcctttttc atactatcta tttcattgct aaaatggtcc aaattatcat
     1021 actcttgttt ccatattaac ctttccagca tggtttgtgt tgtccttaac attttctctg
     1081 cagtttgctt tatcttttca tccagctttg gtggtcttac aaaataattt atagccgtcc
     1141 ctattattac cccaattaag gtatctaagg ttctgttaac tgcataacta agtccatcat
     1201 ccatgtcata gtttaaaact attgccaaaa acacaaccat ggatattctg gctgccttct
     1261 cccagttaaa cttggtacaa atatttataa taaagataag gcctatggcc agggctataa
     1321 aatttccagg gacaattaaa gtaacaatta aggctgtaaa acctcctaaa atagttccat
     1381 acattctatt ggttcctgtg acaaaagatt ctgatatgga agattccata gtcattatgg
     1441 ctgcaatact tgccaataat gggcttctaa gcttaaacac ctccgctact atcagtgtta
     1501 aaataactgc aataacagtc tttactgtcc gcattcctat ttttgcaaat ttcattaata
     1561 tacccctctt aattttatta ttttccatta tattcatagg ttgacaaaaa atcaactggg
     1621 tcaattactt aaaaattctt ccataataaa aataccctaa actaagataa ttatcttaat
     1681 ttaaggcatt agatttatta cttaaattat ataaatatta ttcccattta attactattt
     1741 tttatataag aattcttcca ggtatttgcc tttagaaggg tgcctaagtt tcctcaaagc
     1801 tctttcttga atttgcctta ttctttctct agttaaatca tatttcaagc caatttcttc
     1861 cagtgtgtga ggttcatatc catttaagcc atacctcatt tctaaaatac tttgttctct
     1921 aggcattagg gt
//
LOCUS       DATGSI010000569         1366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_181876, whole genome shotgun sequence.
ACCESSION   DATGSI010000569 DATGSI010000000
VERSION     DATGSI010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181876"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VK031_08780"
     CDS             <1..802
                     /locus_tag="VK031_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54053.1"
                     /translation="EKAEVAKKDMAPNSLQEIHNDLVDILDKVGIIEKLNLGMTIPSE
                     EDEMEIQDIKGKDISDEDGSDDKEKQEYLKNKDMENQGAINENQDNKEEIIRENWLDI
                     DEKLEKVHSTWNKYAVEALEKGAINERINNFNSDLNKATNFIEKREVLDIYNYTSQAI
                     GSLKYFYELYTEEIRGDIAQLQYQVYQYYIMAITGDLQAALASLSNNSEAINRVKLKI
                     KDDEEKLKDIEKISLAINNLSQALQENSRQLFIIKKDIIIEDLKKLAQ"
     gene            1097..>1366
                     /locus_tag="VK031_08785"
     CDS             1097..>1366
                     /locus_tag="VK031_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HLS54054.1"
                     /translation="MKIKILSDSACDLPEDIIKEYDIDILPIIVLKNEEEYLDKVDIE
                     PQEVYDGMREGQVYKTAQISLATFMKKFEEYAEKEIPVIYLAFSSE"
BASE COUNT          575 a          164 c          261 g          366 t
ORIGIN      
        1 ggaaaaggca gaggttgcta aaaaagatat ggcaccaaat tcccttcaag aaatccataa
       61 tgatctagta gatatactgg ataaggtagg tataattgaa aaattaaacc tagggatgac
      121 cattccctca gaagaagatg agatggaaat acaggatatt aaggggaaag atataagtga
      181 tgaggatggt agtgatgata aggaaaagca ggaatatttg aaaaacaaag atatggaaaa
      241 ccaaggtgca atcaatgaaa accaagataa taaggaagaa atcataaggg aaaattggct
      301 ggatatagat gaaaagttag aaaaagttca ttctacctgg aacaaatatg cagttgaggc
      361 cctagagaag ggggcaatca atgaaagaat aaataatttt aactcagatc taaataaagc
      421 gactaatttt atagaaaaaa gggaagtatt agatatatat aactatacta gccaagccat
      481 aggaagtttg aagtattttt atgagctgta tacagaggaa ataagaggag atatagccca
      541 gctacagtat caagtatacc aatactacat tatggccatt acaggagact tacaggcggc
      601 actagcctct ttaagcaata atagtgaggc tataaataga gtgaagctaa agataaagga
      661 tgatgaggaa aaactaaaag atatagaaaa aatatccttg gccataaaca atctatctca
      721 agctttgcag gagaatagta gacagctttt tataataaaa aaggatataa taatcgaaga
      781 tttgaaaaaa ttagcacagt agaaaatagc agataagtct gctattttta aaaacctatt
      841 atatgtagtt taaaaaatag ttatttgaag atagataaaa gtattagaca aaaaattaca
      901 atcataatta ttgcgattaa ccaatttaga acccttttac ttataaagaa aaatctcata
      961 tatgaccacc ccttttaaca tacctaatat aatctattta actagtgcaa attatagacc
     1021 tgtattttta aatagctttg tttttttggt aatttattgt attattattg tataagtaaa
     1081 aatggaggga agagtagtga agatcaaaat actaagcgat agcgcctgtg acctaccgga
     1141 agatataatc aaagaatatg acatagatat tttacctatt atagtattaa aaaatgaaga
     1201 agagtatttg gacaaggtag atatagagcc tcaagaagta tatgatggaa tgagagaggg
     1261 acaggtctat aaaacagccc agattagttt agcaaccttt atgaaaaagt ttgaagaata
     1321 tgcagaaaaa gaaatacctg taatttactt ggccttctct tcagaa
//
LOCUS       DATGSI010000570         1352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_560174, whole genome shotgun sequence.
ACCESSION   DATGSI010000570 DATGSI010000000
VERSION     DATGSI010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560174"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VK031_08790"
     CDS             <1..458
                     /locus_tag="VK031_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54055.1"
                     /translation="TRNDYLNPILDVTIEHCEPNYMYDYRDYIGLSTSTSLGIGSSNV
                     IGEYKVDMDKRLIVLNPEFTYSCIVMKYLKSSTPDGEYFINELASEALLAYIEYVWHR
                     GRNVPMSTKQDLERRWYNEKRKAKRRIKPITKSDLNHNSRQSAKGSLKM"
     gene            462..>1352
                     /locus_tag="VK031_08795"
     CDS             462..>1352
                     /locus_tag="VK031_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54056.1"
                     /translation="MGEIVEQKILVGGMDSDNSIESVREDKYLTAHNYRPTGTDQQDR
                     GYGNNIESTVLIDTDTAPSTGINKDLKIESFETIGKVISIMYNSQGYHQIREYDYDTN
                     SINVIFENLTDTDGIDILRLTPQSFVNDLRLVQERYIVITDGVNEIYCFDREELLKGY
                     GRPINIEDITLYKPNPKHEPKSEYRDDENYSTNNLRGKLFQFRSQYKYGGNMFSAWSP
                     MSEREVPSTEASVITGLAVGKDNVMVITVKLESDSVERVLVSSRFNDSDWFIIKDVST
                     EHIKSLSSTPINLTTVTETYD"
BASE COUNT          524 a          212 c          269 g          347 t
ORIGIN      
        1 ttacaagaaa tgattattta aaccctatat tagatgttac aatagaacac tgtgaaccta
       61 attatatgta cgattacaga gattacatag gacttagcac aagtacatct cttgggatag
      121 ggtcttcaaa tgtaataggc gagtacaaag tggacatgga caaaagactt attgttttaa
      181 atccagaatt tacttattca tgtatagtaa tgaaatactt gaaaagtagt acaccagacg
      241 gagagtattt tataaacgag ttagcaagtg aagctttact ggcatatata gagtacgtat
      301 ggcatagagg taggaatgtt cctatgagta ccaaacagga tttagaaaga agatggtaca
      361 acgaaaagag aaaagctaag agaagaataa aacctatcac aaaatcagac ttaaaccaca
      421 acagcagaca gtcggctaag ggaagtttaa aaatgtaact tatgggagag atagtagaac
      481 agaaaatatt agtcggagga atggactctg ataactctat tgaatcagta agagaagaca
      541 aataccttac tgcccacaat tacagaccta ctggtacaga ccagcaggac agagggtatg
      601 gaaataacat agagtctaca gtacttatag acacagacac agcaccgagt acaggtataa
      661 ataaagacct caaaatagaa tcttttgaga ctataggaaa ggtaatatct attatgtaca
      721 acagtcaagg gtatcatcag ataagagagt acgactacga taccaactct ataaatgtga
      781 tatttgaaaa ccttactgac actgacggaa tagacatttt aaggctgacc ccacagtctt
      841 ttgtaaacga tttaagactt gtacaggaaa ggtatatagt cataacagac ggagtaaacg
      901 agatatactg ctttgacagg gaagaactac ttaaagggta tggcagacct attaatatag
      961 aagacataac actatataaa cctaatccta agcacgagcc taaatcagaa tacagggatg
     1021 acgaaaacta ctctacaaac aaccttagag ggaagttatt tcagttcagg tctcaataca
     1081 agtacggtgg aaatatgttt tcagcttgga gtcctatgtc tgaaagagaa gtaccaagta
     1141 cagaagcttc tgtaattaca ggacttgcgg taggtaagga taacgtaatg gttattactg
     1201 taaaattgga aagtgattca gtagaaagag ttttagtttc ttcaaggttt aacgactctg
     1261 actggtttat tataaaagat gttagtacag aacacattaa aagtttatcc agcacaccta
     1321 taaatttaac cactgtaacc gaaacctatg at
//
LOCUS       DATGSI010000571         1731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_229035, whole genome shotgun sequence.
ACCESSION   DATGSI010000571 DATGSI010000000
VERSION     DATGSI010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229035"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1378)
                     /gene="mtaB"
                     /locus_tag="VK031_08800"
     CDS             complement(77..1378)
                     /gene="mtaB"
                     /locus_tag="VK031_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HLS54057.1"
                     /translation="MKKVAFHTLGCKVNQYETEAMEELFEKANYSIVRDEEFAHIYVI
                     NTCTVTNLSDRKSRQFIRRVRRINKDATIAVVGCYSQISPEEISKIEGVDVILGTSQR
                     NRIVELCEEAEKKNRQINIVESARSYQEFEEISVEDIKSKTRAYIKIQDGCNQYCSYC
                     IIPFARGPIRSRRLEDTMVEIEKLADSGFKEIVLTGIHVASYGKDLGDINLDQAIKKI
                     STIAGIERIRLSSVEPNIVDDEFMTTIVETGKACDHFHLSLQSGSDKILRRMNRKYTC
                     NSFKEKVELIRKYMPDAGITTDIIVGFPGETDKDFEETLDFVKDIGFSRIHVFKYSPR
                     EGTAAARFKEQVNGKVKNERSRILIHLADQLSREFNSRFINSHMEVLYEVEDENNPGV
                     FEGYTSNYIRVKTKSDEDLVGQILRTQIVDSKGDILIGKLI"
     gene            complement(1378..>1731)
                     /locus_tag="VK031_08805"
     CDS             complement(1378..>1731)
                     /locus_tag="VK031_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HLS54058.1"
                     /translation="TIAQEAAKQAKRDYIPEVKGILTFEEMLECLKGEDNIIVPYEAE
                     ENNSIGHNLRNIKNGRINLIIGPEGGFEKEEIKRLEEYGAKIVTLGPRILRTETAGLV
                     AATIILYKLGDLGVI"
BASE COUNT          486 a          393 c          215 g          637 t
ORIGIN      
        1 actaggaata tcaccattga ctattttgca aaatatacag ttatccatgg attcacctcc
       61 tatggtctta aatttattag attaactttc ctattaaaat atcaccttta ctatccacaa
      121 tctgtgttct taatatttgg ccaaccaagt cttcatctga cttggttttg accctaatat
      181 aattactagt atacccttca aaaactccag gattattttc atcttcaact tcatatagaa
      241 cttccatatg ggaattgata aatctactat taaattccct gctcagctgg tcagccaggt
      301 ggattaagat cctacttctt tcatttttaa ccttaccatt tacctgctcc ttaaatctgg
      361 ctgccgctgt accttccctt ggagaatact taaatacatg gattctagaa aaaccaatat
      421 cctttacaaa atctaaggtt tcttcaaaat ctttatcagt ctctcctgga aaacctacaa
      481 taatgtcggt ggtaattcca gcatcaggca tatattttct aatcaattcc accttttcct
      541 taaagctatt gcaagtatac tttctgttca tccttcttaa gattttatca gaaccacttt
      601 gcagagacaa atggaaatgg tcacaagcct taccagtttc aactatagta gtcataaatt
      661 catcgtcaac tatattaggt tccacagaac ttaacctaat cctttctata cctgctatag
      721 tggaaatctt ttttatagct tggtctaagt ttatatcccc taagtccttt ccataggagg
      781 ctacatgaat accggtcagt actatttcct taaaacctga atctgccagt ttctctatct
      841 caaccatagt atcttctagc cttctacttc ttatgggccc tctagcaaag gggataatac
      901 aatatgaaca atactgatta catccatcct gaatttttat ataggctcta gttttagatt
      961 ttatatcttc cacactaatt tcttcaaatt cttgatagct tcttgcactt tctactatat
     1021 tgatctgcct atttttcttt tcagcttcct cacatagctc cacaatccta tttctttggc
     1081 tggtacctaa aattacatct acaccctcaa ttttagaaat ttcttcagga gatatttggg
     1141 aatagcatcc aactacagca attgtagcat ccttatttat ccttctgacc cttcttataa
     1201 attgtctgct tttcctgtct gaaaggttgg ttacagtgca ggtattaata acatatatat
     1261 gggcaaattc ctcatctctt acaatagaat aatttgcctt ttcaaaaagc tcttccatag
     1321 cttcagtttc atactggttt accttacacc ctaaggtatg aaaggctact tttttcatta
     1381 aatcactcct aaatccccca gcttatataa aataatagta gctgccacaa gtcctgcagt
     1441 ttcggtccgt aatatcctag gtcctagggt aactatcttg gccccgtatt cctccagcct
     1501 ttttatttct tctttttcaa atccaccttc tggaccaata attagattga ttctaccatt
     1561 tttaatattt cttaaattat ggcctatact attattttct tcagcctcat agggtactat
     1621 tatattgtcc tcgcccttta agcattcaag catttcttca aaggttaaaa tacccttaac
     1681 ttcaggtata taatctctct ttgcctgttt agctgcctct tgggcaatag t
//
LOCUS       DATGSI010000572         4463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_86367, whole genome shotgun sequence.
ACCESSION   DATGSI010000572 DATGSI010000000
VERSION     DATGSI010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86367"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..554
                     /locus_tag="VK031_08810"
     CDS             249..554
                     /locus_tag="VK031_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLS54059.1"
                     /translation="MRKYRIVNKTRFTLFILSLIVLIYLVVLLTSTVVRAQEFKLAKK
                     HLEYQIKKGDTIWNIALKYKPDKYDVRKMVYDIKEYNQLDNYYLYPGDTIKVPLHEK"
     gene            complement(608..1582)
                     /locus_tag="VK031_08815"
     CDS             complement(608..1582)
                     /locus_tag="VK031_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HLS54060.1"
                     /translation="MSEIPIVLEDYLNYMEMIKGSSPNTVREYFFDLRTFLKYLKVRF
                     KLVDSDQEFEEIDISDIDENFLKKVTIQDLHAFISFIDKERDNKNHAKSRKVASLRSF
                     FDYLHSKVNLIDKNPAASLEFPKINSRHPVYLTLEESQHLLNTVLENKNEEYRKRDYA
                     IIMLFLNCGLRLSELSSINIDSIKNDDALSIIGKGNKERTIFLNQACIDAINDYLRVR
                     PTNVNDDKALFISKRKNRMSNRAIQHMIDRYLSRAGFDTSVYSTHKLRHTAATLMYKH
                     GNVDIRALQELLGHESVSTTQIYTHIDDERLRNAVQSNPLANVNNKEK"
     gene            complement(2129..2512)
                     /locus_tag="VK031_08820"
     CDS             complement(2129..2512)
                     /locus_tag="VK031_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HLS54061.1"
                     /translation="MSIYKGERHRQFPTTIGTTSFVRLYILHLLTEKSYYGNKIREEI
                     TERLDHKWSPSPGMIYPLLRELESKGYIEGRWDEPEKRSIRRYTITDNGLRYYKNILR
                     KYKTSFDDSLLIIKNVLKDIYKEEI"
     gene            complement(2513..3331)
                     /locus_tag="VK031_08825"
     CDS             complement(2513..3331)
                     /locus_tag="VK031_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HLS54062.1"
                     /translation="MKFDMHVHTYYSDGIFSPKEVVEKAVQLGLDGIAITDHDTVLGV
                     KEAIDASKEYPNFSVIPGIEFSCIHEDEEVHILGYFINYESLELEKILDKLNSARRNR
                     TGKIVAKLNQLGMDIEEKDVLEIGKKNFTGRVHVAKALIKKGYVSSVEEAFEKYLERG
                     QPAYVERESLSINKAIRLINSLGGVSILAHPGLLKKRDKIIKYCIDAGILGLECIHSK
                     HRKEDVEGLKRIVQENNLIATGGSDCHGRKIDGEILLGRYYCDLNKIPEMKGRL"
     gene            complement(3391..3651)
                     /locus_tag="VK031_08830"
     CDS             complement(3391..3651)
                     /locus_tag="VK031_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HLS54063.1"
                     /translation="MDILKVSAKSIPNSVAGALAGVLREKGTAEIQAIGAGALNQAIK
                     AVAIARGFVAPSGMDLICIPAFTDIQIDGDERTAIKLIVEPR"
     gene            complement(3711..>4463)
                     /gene="rny"
                     /locus_tag="VK031_08835"
     CDS             complement(3711..>4463)
                     /gene="rny"
                     /locus_tag="VK031_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HLS54064.1"
                     /translation="EKLIVDGRIHPARIEEMVDKAKKEVNTIIREEGEQAAFDTGIHG
                     LHPEIIKLLGRLKYRTSYGQNVLNHSIEVAHLAGIMAAELGADIKIAKRAGLLHDIGK
                     AVDHEVEGPHVDIGVDLARRYRESKEVLHAISAHHGDIEPTTVEAVLVQAADAISAAR
                     PGARRETLEAYIRRLEKLEEIANSIEGVEKSFAIQAGREIRIMVKPEVIDEIQTVHTA
                     REIVKKIESELDYPGQIKVNVIRETRAIEYAK"
BASE COUNT         1347 a          839 c          623 g         1654 t
ORIGIN      
        1 aagggggata cccctgcctc tgaatttcac ttttaataaa aagtaaaatt tctacctgct
       61 tctgatttag gtcttcatac atagtcaaca ccccttcaca tataatttta ttaatgatat
      121 tatagcatat ttattcataa atatcaaaca ggggttctta aattttgcaa atactattga
      181 caaagaacct atgtttatgt acaatataat tgaactattg ttcagaacta acgtgcgagg
      241 tgtttgggat gagaaaatat aggattgtta acaagaccag atttacttta tttattctca
      301 gtctaattgt acttatttac ttagtagttt tattaacctc aactgtggta agggcccagg
      361 agtttaaact ggctaagaaa catttagagt atcaaattaa aaaaggggac accatatgga
      421 atattgctct aaagtataag ccagataaat atgatgtaag aaaaatggta tacgatataa
      481 aagaatataa tcaactggac aattattatc tctaccctgg agatactata aaggtccctt
      541 tacatgaaaa ataggctgat tgtggaatca gcctattttt atagcataat ataatttata
      601 ctttaaatta tttctcttta ttattcacat tagctaaagg attactttgg actgcattcc
      661 gtaacctttc atcatcaata tgagtatata tttgagtggt agatactgat tcatggccta
      721 acaattcctg aagagctctt atatccacat tcccatgctt gtacataaga gtagctgccg
      781 tatgtctcaa cttatgggta gaatatacac tagtatcaaa cccagcccta gatagatacc
      841 tgtcaatcat atgttgaata gccctattac tcatccgatt tttcctttta ctaataaaca
      901 aggccttgtc atcgttaaca ttagtgggcc ggacccttag gtaatcgtta atagcatcta
      961 tacaggcctg atttaaaaag atagttcttt ctttatttcc ttttcctatg atagagaggg
     1021 catcatcatt ttttatacta tcaatgttta tactagataa ttcagataga cgaagtccac
     1081 aatttagaaa aagcattata atagcataat ctcttttacg atattcctcg tttttatttt
     1141 caagaactgt atttaataga tgttgagatt cctccaaggt caagtatact gggtgtctag
     1201 agtttatttt aggaaactcc aggcttgctg caggattctt atcaatcaaa tttacctttg
     1261 agtgcagata gtcaaagaag gacctgaggg aggcaacctt tcttgattta gcatgatttt
     1321 tattatctct ttctttatct ataaaagaga tgaaggcatg aaggtcttgg atagttactt
     1381 tttttaaaaa attctcatca atatctgata tgtcaatttc ttcaaattcc tgatctgaat
     1441 caacaagctt aaatctaacc tttaagtatt ttagaaaagt tctaagatca aaaaaatatt
     1501 ccctaacagt attaggggaa gatcctttga tcatttccat ataatttaaa taatcttcca
     1561 aaacaatagg tatttctgac ataaatatcc tccttaagta tatttaaagc aaaattaagt
     1621 gatttttact gtatattagt cacaaaatag ttattttgtg actaatcatt tttctaagta
     1681 aattatagta tattttggga taaattgcaa attagttttt tatacaaatc tattcttttt
     1741 ccttataatc taattgttat taaccaaata taaaaactat ccatcaattg tctaacttgc
     1801 taaactaaat atgtatattc cctacatttt aagtttttta taaactttac atgatttttt
     1861 aatttaattt caaggaaaaa tctaaaaaag tgctcaaggc cccacaattg ccttcagggg
     1921 agttttggtg gcgtattcat gtcaatgtat ggaaggtaaa agaaaggccc cttacagggc
     1981 gttttgaagg gccgtttttc gaaaacttta agaaaatata attatatttc tattggcagt
     2041 caaaagctta tataaaatgg ctagatatgt aaatttatat tatttatata ttctagttat
     2101 ataatgtgta taaaaatact ttcaataatt aaatttcctc tttataaata tcttttaaaa
     2161 cattttttat aattagtagt gaatcgtcaa aggaagtttt gtacttacgt agaatattct
     2221 tataatacct aagtccattg tctgtaattg tatatcttct tatagacctc ttttccggtt
     2281 catcccatcg cccttctata tatcctttgg attctagctc tcttaacaaa ggatatatca
     2341 ttccagggct tggactccat ttatgatcta atctttctgt aatctcctct ctaattttat
     2401 ttccgtaata acttttttca gtcaataggt gtaaaatata taatctaaca aaagaagtgg
     2461 ttcctatagt cgttgggaat tgtctgtgcc tttctccctt ataaatactc atctataacc
     2521 ttcctttcat ttcaggtatt ttgtttaaat cacaataata tctgcctaat aagatttccc
     2581 catcaatttt ccttccatgg caatcagatc cccctgttgc aattaaattg ttttcttgga
     2641 caattctctt aagcccttcc acatcttctt ttctgtgttt agagtggatg cattctaaac
     2701 ctaagatacc tgcatcaatg caatatttta taattttatc tcttttcttt aataagccag
     2761 gatgtgctaa tatggataca cctcctaggc tattaattag tctaatagcc ttgttaatac
     2821 ttaagctttc cctttcaaca taggctggtt ggcctctttc taaatatttt tcaaaggctt
     2881 cttccacgct ggatacatat ccttttttta ttaaagcctt ggctacatgg actctaccag
     2941 tgaaattttt cttgcctatt tctaaaacat ccttttcttc tatatccata cctaattgat
     3001 ttaatttagc tacaatcttt ccagttctat ttctcctggc ggaatttaat ttatctaaaa
     3061 ttttttctag ttctaaggac tcataattga taaagtaacc taaaatatga acttcttcat
     3121 cttcatgaat acaactgaat tcaattcctg gaattacaga aaagttggga tattcttttg
     3181 atgcatctat tgcctcttta actcccaata cagtatcatg atcagtgatt gcaattccat
     3241 ctaggcctaa ctggacagct ttttcaacta cttcctttgg gctaaatatc ccatcagaat
     3301 aataggtgtg tacatgcata tcaaacttca agtatgtcta cctcccattt attttgaata
     3361 aaaaaacctg ccatatggca ggttttgtca ttatcttggt tcaacaatca attttattgc
     3421 cgttctttcg tcaccatcaa tttgaatatc tgtaaaagct ggaatacaga taagatccat
     3481 tccgcttggc gctacaaatc ccctagctat agctaccgct ttaattgcct ggtttaatgc
     3541 ccctgcccct atggcttgta tttctgcagt tcctttttct ctaagcacac cagccaatgc
     3601 acctgcgaca gagtttggga ttgattttgc tgatactttt aatatatcca tttaatagcc
     3661 cccttatact aatttaatat attttttatt atatattata ttcaaattaa ttatttagca
     3721 tattcaatag ctcttgtttc acgaataacg ttaaccttga tttgaccagg ataatctaat
     3781 tctgattcaa ttttcttaac aatttcccta gccgtgtgaa ctgtctgtat ttcatcaatg
     3841 acttcaggtt ttaccataat tcgaatttca cgcccagctt gtattgcaaa ggatttttca
     3901 actccttcga ttgaattagc tatttcttca agcttttcca atctccttat ataggcctca
     3961 agggtttctc gccttgcacc tggtctagcc gctgatatgg catcggctgc ttgtaccaaa
     4021 actgcttcta cagtagttgg ctcaatgtct ccatgatggg ctgatatagc gtgaaggact
     4081 tccttagatt ctctatatct tcttgctaaa tcaactccta tgtcaacatg aggaccttct
     4141 acctcgtgat ccaccgcctt acctatgtca tgcaataagc ctgcacgttt agcaatcttt
     4201 atgtcagctc ctaactcagc cgccataata cctgctaaat gtgccacctc gattgaatgg
     4261 ttcaatacat tttgtccata actagttcta tacttcagtc tacctaataa ttttatgatt
     4321 tctggatgaa gtccatgaat accagtatca aatgctgcct gttctccttc ttctctgata
     4381 atagtattga cttccttttt agccttgtca accatctctt caattctagc cggatggatt
     4441 cttccatcta ctattaattt ctc
//
LOCUS       DATGSI010000573         1652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_462781, whole genome shotgun sequence.
ACCESSION   DATGSI010000573 DATGSI010000000
VERSION     DATGSI010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462781"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VK031_08840"
     CDS             complement(<1..342)
                     /locus_tag="VK031_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54065.1"
                     /translation="MKTNIKDLEVLEKYLSEEELKEVAKEVAYNAFKSSLGVENPFSA
                     SNLNFYTSQGAYEAVKQHITDNDFREEEFVEELRSRVSSVIKGLNYYQLPLDEVIEEV
                     VDEKKGEIRDRI"
     gene            complement(365..952)
                     /locus_tag="VK031_08845"
     CDS             complement(365..952)
                     /locus_tag="VK031_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54066.1"
                     /translation="MKKYEDKLKAHPVVRDLIEVAKKHNTRSAYLTGGAVVDILDGRE
                     PKDYDISWIACQNIHETLGKHKYFKLIYSSRTSTTYLFKEKYKVQILKTHERDFPFMN
                     DRILFNLRGELEIRDNPYLLSILKERIIVVNDEMFERGGISRKLFKARVKKWESKGYK
                     MHPITYKSYIRMTKHKSIFRRFVEVFTGSKSETES"
     gene            complement(1108..1341)
                     /locus_tag="VK031_08850"
     CDS             complement(1108..1341)
                     /locus_tag="VK031_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HLS54067.1"
                     /translation="MEVKTWKYKMPFGKYKGKTLEEIYKRDKSYLAWMYSKEVLKFSP
                     ELAKETSKIKLESLYPSKWEFPHWADDMGYYIG"
     gene            complement(1366..1620)
                     /locus_tag="VK031_08855"
     CDS             complement(1366..1620)
                     /locus_tag="VK031_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54068.1"
                     /translation="MSEQIGKYYTDNNGYVRWELERLGFNHLPIPVTGEYLYSFISRH
                     ANSSEEWIEKNYVLFNKEDKKEIVTQYLPNEEYLIRIACL"
BASE COUNT          436 a          350 c          210 g          656 t
ORIGIN      
        1 aattctatct cttatctccc ctttcttttc atcaacaact tcttctataa cttcgtctaa
       61 aggcagttgg tagtaattta accccttaat aaccgaggat actcttgatc taagctcttc
      121 tacgaactct tcttctctga aatcattatc tgtaatgtgt tgcttaacag cttcgtaagc
      181 gccttgagat gtgtagaagt tcaagttact tgcactgaaa gggttctcaa cccctaaaga
      241 acttttaaat gcgttatacg ctacctcttt agcaacttct tttaattctt cttctgatag
      301 gtatttctct aatacttcta aatctttaat gtttgttttc atatctataa atatttatgt
      361 gtgtttaact ttctgtttct gatttactac ctgtgaatac ttctacaaac ctacgaaaga
      421 tactcttatg ttttgtcatc cttatgtaac tcttgtaagt tatcggatgc atcttatatc
      481 ctttactctc ccacttttta acacgagctt taaataactt tctagatatt ccacctcttt
      541 caaacatctc atcatttact actataatgc gctcctttaa tattgaaagt aagtaaggat
      601 tgtctcttat ctctaactct cctcttaggt taaatagaat tctatcgttc ataaagggga
      661 aatccctctc atgagttttc agtatttgca ccttatactt ttccttaaac aaatatgtcg
      721 tggaagttct ggagctatat attagtttga agtatttatg tttacccaag gtttcgtgta
      781 tgttttgaca agctatccat gaaatatcgt agtccttagg ctctctacca tctaatatat
      841 ccactactgc tccgcctgta aggtaagcac tccttgtatt atgtttctta gcaacttcta
      901 ttaagtccct cactactggg tgagctttta atttgtcttc gtattttttc ataatttata
      961 tcttttattt gcctaatccc ttttatttaa ttctctcatc actatccatc ctacgtatat
     1021 aacgaatcct aatgggatta ctaccatagc gaggacacct attaaaatta atgttattat
     1081 tgttttcatc gtttttatat ctgtttgtta accaatgtaa taccccatat cgtctgccca
     1141 gtgagggaac tcccatttag aggggtacaa agattctaat ttaatcttac ttgtctcctt
     1201 agctaactct ggactaaatt taagaacttc tttggagtac atccatgcta aataactctt
     1261 atcccttttg taaatctctt ctaaggtttt ccctttgtac ttaccgaaag gcatcttata
     1321 cttccaagtc tttacttcca taattatttt acttttaaaa attatctata aacatgcgat
     1381 tcttattaaa tactcctcgt taggtaagta ttgtgtaaca atctctttct tatcctcttt
     1441 gttaaaaagt acgtagtttt tctcaatcca ttcttccgag gagttagcgt gtcttgatat
     1501 gaaagagtat aaatactccc ctgtcactgg aattggtaag tggttaaacc ctaacctttc
     1561 taattcccac cttacgtacc cgttgttatc tgtatagtac ttacctatct gttcactcat
     1621 cttctcctcc tacttctatt tcgattgttc ca
//
LOCUS       DATGSI010000574         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_229215, whole genome shotgun sequence.
ACCESSION   DATGSI010000574 DATGSI010000000
VERSION     DATGSI010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229215"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VK031_08860"
     CDS             complement(<1..132)
                     /locus_tag="VK031_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HLS54069.1"
                     /translation="MRNDNFKLISIGLVAGLINGLFGSGGGTIVVPALVFIMGLEDHK
                     "
     gene            222..953
                     /gene="nadE"
                     /locus_tag="VK031_08865"
     CDS             222..953
                     /gene="nadE"
                     /locus_tag="VK031_08865"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HLS54070.1"
                     /translation="MDKMDLLVKDLVSWLKVHVEGAGGKGLVFGLSGGVDSAVMAAIS
                     KLAFPDTSLGIIMPCYSDPRDKEDALLVADALDLKTQVVDLGPVFDIFIKQLKLQKSK
                     LAEANLKPRLRMSTLYYYAQNLNYLVAGPTNKSEFHVGYFTKHGDSGVDLLPLANIVK
                     EDIWNMARYLKVPEPIIEKTPTAGLWEDQNDEEEMGFSYKLLDKFLRTGKGPENIIEK
                     IDKMNITSSHKRKFPPIYKYHNLVD"
     gene            987..1712
                     /locus_tag="VK031_08870"
     CDS             987..1712
                     /locus_tag="VK031_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLS54071.1"
                     /translation="MAGHSKWSNIKRRKGKEDAKRAKVFTKLARYIMVAAREGGPDPE
                     FNPALKAAIDKAKAENMPNDNIERAIKRGSGELGADNYEEITYEGYGPSGVAVFATCL
                     TDNRNRTASEIRHAFDKFGGNLGQSGSVTFMFDRKGIIGIEKNDKIDEEELTLLAIEL
                     GAEDFEEQEEGYEIITDPKDFARVKEGLEEAGYSFAIVELGYIPQTTVSLEDEEDIKN
                     MENLIDTLEDNDDVQEVYHNWEN"
BASE COUNT          695 a          271 c          417 g          534 t
ORIGIN      
        1 cttgtgatct tctagaccca ttataaatac taatgctgga actactatgg ttcctcctcc
       61 tgagccaaat agaccattta taaggcctgc tactaggcca atgcttatta atttaaaatt
      121 atcatttctc atatggctct cctttgtggt ataataaaaa aagtatttgt attaatttca
      181 ccagaattat gtaagatata catagggagg ataaggcctt tatggataaa atggatttgc
      241 ttgtgaaaga tttggtaagc tggttaaaag ttcatgtaga aggtgctggt ggaaagggat
      301 tggtatttgg tttaagtggt ggagttgatt ccgctgttat ggctgccata tcaaaactgg
      361 cctttccaga tacttctttg ggtataatta tgccctgcta tagtgaccct agggataaag
      421 aggatgcttt actggtggca gatgccttag acctaaagac tcaggttgtt gatttaggac
      481 cagtttttga tatttttata aagcagctta aacttcaaaa aagtaaactg gcagaagcca
      541 acctaaaacc taggttaaga atgagcacct tgtattatta tgctcaaaat cttaactatt
      601 tagtggctgg acctactaat aaatctgagt ttcatgtagg ctactttacc aaacatggag
      661 acagtggagt agatttgctt cctttagcca acatagttaa ggaagatata tggaatatgg
      721 ctagatactt aaaggtacct gagcccatta tagaaaaaac tcctactgct ggcctatggg
      781 aggatcaaaa cgatgaagaa gagatgggct tttcctataa gctattagat aagttcctta
      841 ggaccggaaa aggacctgaa aacattatag aaaagataga taaaatgaat ataacaagta
      901 gtcacaaaag aaaattcccc cctatttaca aataccataa tctggtggat tagatatttt
      961 atataattta taaggaagtg aaagtaatgg caggacattc taaatggagt aatattaaac
     1021 gaagaaaagg taaggaagat gcaaaaaggg ccaaagtatt tacaaaacta gctagataca
     1081 ttatggttgc agcaagggaa ggtgggccag acccagaatt taatcctgct ttaaaggcgg
     1141 ctatagataa agcaaaggca gaaaatatgc ctaatgataa tattgaaagg gctattaaga
     1201 gagggtctgg tgaattaggg gcagacaatt atgaagaaat tacctatgaa ggctacggtc
     1261 cttcaggtgt tgctgtgttt gccacttgtt taacagacaa tagaaatagg acagcctctg
     1321 aaataagaca tgcctttgat aaatttggtg ggaatttagg acaatcaggg tcagtaacct
     1381 tcatgtttga tagaaaagga ataataggta tagaaaaaaa tgataaaata gatgaagaag
     1441 aactaactct cttggccata gaattagggg cagaagattt tgaagaacag gaagaaggtt
     1501 acgaaattat aactgatcca aaggactttg caagggttaa agaaggattg gaggaggcag
     1561 gctatagctt tgccattgtg gagctgggct atatacctca gactactgtg agtttggaag
     1621 acgaagaaga tataaaaaat atggaaaatt taatagatac ccttgaagat aacgatgatg
     1681 tacaagaagt ataccataat tgggaaaatt agaatggctt aatcctgtat aagtgaaaat
     1741 cctaactgac ataataataa caaatacacc tatgtaagga ggttgcttat ggagggaaaa
     1801 gaaaatttcc aacatattct agaaagattt caggaagctg atttagaaca aaaaatagaa
     1861 atatatacca ccgttagagg attaagtgta gaacaatata aggagctact cagatat
//
LOCUS       DATGSI010000575         1315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_136113, whole genome shotgun sequence.
ACCESSION   DATGSI010000575 DATGSI010000000
VERSION     DATGSI010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136113"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VK031_08875"
     CDS             complement(<1..702)
                     /locus_tag="VK031_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLS54072.1"
                     /translation="MDLNLKDKVYIVTGGFKGIGRAIALQLAKEGAIPVVLNRKNNVA
                     EEFENTLKEITDTYSIYYIDLNDTQSIEGIVKDVVDKYGKINGIVNNAGRNDNLSLEE
                     TSWQEFEASIHSNLTHYYELVHQAVSYLKESKGAVVNISSKTAVTGQGKTSAYAAAKG
                     AILGLTREWAAALAKDSVRVNAIVVAESWTPLYKDWIKTFGDEEEQEKRLKLITDNIP
                     LEKRMTYPEEIANETV"
     gene            complement(724..>1315)
                     /locus_tag="VK031_08880"
     CDS             complement(724..>1315)
                     /locus_tag="VK031_08880"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009352376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HLS54073.1"
                     /translation="GLTAATGMDALTHAIEGYITKDANELSDTFHLKSIELIANSLKK
                     ACENDKDARERMALAQYVAGMGFSNVGLGLVHSMAHPLGALYDTPHGVANAILLPTVM
                     EYNAEETGEKYREIARVMGVKDVDKMSQDEYRKAAVDAVKDLSKAVDIPEDLKDIVKK
                     EDIDFLAQSAMDDACRPGNPKDPTKEEVIGLYEKLI"
BASE COUNT          325 a          262 c          201 g          527 t
ORIGIN      
        1 tacagtttca tttgcaattt cttcaggata ggtcattctc ttttctaacg gtatattgtc
       61 tgttattaat tttagtcttt tttcttgttc ttcttcatca ccaaatgtct ttatccaatc
      121 tttataaagt ggtgtccaag attcagcaac tactatagca ttaactctta cactatcttt
      181 ggctaaggct gctgcccatt ctcttgtaag acctaggatt gcaccctttg ctgctgcata
      241 ggctgatgtt tttccttggc ctgtaactgc agtttttgaa gaaatattaa caactgctcc
      301 cttgctttct tttaggtagg aaactgcttg gtgaacaagt tcataataat gagttaggtt
      361 tgaatggatt gatgcttcaa attcttgcca gcttgtttct tcaaggctta agttgtcatt
      421 tcttccagca ttgttaacta ttccatttat ttttccatat ttatcaacaa cgtctttaac
      481 gattccttca attgattgag tatcgttaag gtcaatataa taaattgaat atgtatcagt
      541 aatttctttt agggtatttt caaattcttc tgctacatta ttttttctat taagtacgac
      601 aggaattgct ccttctttgg caagttgtaa ggcaattgcc cttcctattc ctttgaaacc
      661 gcctgttact atatatactt tatcttttaa atttaaatcc atttcattct cctttttata
      721 tttttatatt aacttttcat aaagacctat aacttcttct tttgttgggt cttttgggtt
      781 tcctggccta catgcatcat ccatagctga ttgggctagg aagtctatat cttctttttt
      841 aactatatct tttaaatctt caggaatatc tactgctttt gataagtcct ttacagcatc
      901 tactgcagcc tttctatact catcttggct cattttatcg acatctttaa cgcccataac
      961 tcttgctatt tccctgtatt tttctccagt ttcctcagca ttatattcca taactgttgg
     1021 aagtaatata gcatttgcaa ctccgtgtgg agtgtcatat aaggctccaa gtgggtgggc
     1081 cattgagtgt actaaaccaa gtcctacatt tgaaaaaccc attcctgcta catattgggc
     1141 aagcgccatt cgctctcttg catccttgtc attttcacaa gcttttttta aggaatttgc
     1201 tataagttct attgatttta agtggaaggt atcagaaagc tcattggcat cttttgtaat
     1261 atatccttct atagcatgag ttagagcatc cattcctgta gcagctgtaa ggcca
//
LOCUS       DATGSI010000576         1469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_229323, whole genome shotgun sequence.
ACCESSION   DATGSI010000576 DATGSI010000000
VERSION     DATGSI010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229323"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..540
                     /locus_tag="VK031_08885"
     CDS             256..540
                     /locus_tag="VK031_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54074.1"
                     /translation="MIHFNQNTRLRMDATIQVKAEDYDKDEGIEALSEFYKLIGHKLH
                     ELGYLEITAQELRVDEDINVQYEGEILLPRNGEDDDDDDGELIVDKSFTF"
     gene            551..925
                     /locus_tag="VK031_08890"
     CDS             551..925
                     /locus_tag="VK031_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54075.1"
                     /translation="MKLGRLLCSMSIHGIMRREEIFRKEPTYPERLKNEEDRINAIVS
                     QYRMTSTSVKPYEKEYWGSSEEERIKEHRNLSKELLDKKVSDYYYRTNLDVELDYGGG
                     HKVTFLADSIKIEDGKLTIKAK"
     gene            929..1129
                     /locus_tag="VK031_08895"
     CDS             929..1129
                     /locus_tag="VK031_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54076.1"
                     /translation="MELVGIIVYMISVCICFKEVSKVQIRDFEGKGYIVSILLGIVGI
                     IPMLNTITATLIVMYNLEMNKD"
     gene            1132..1398
                     /locus_tag="VK031_08900"
     CDS             1132..1398
                     /locus_tag="VK031_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54077.1"
                     /translation="MNSSNLKVGDSCEVVKVLTGGGYPKGTFKVGRDTVANLVYESDR
                     GEESYITLYNSLLNGEVSIAGMSKLTMFRNELKFIGRLVVTKVK"
BASE COUNT          566 a          175 c          339 g          389 t
ORIGIN      
        1 catggcatag tgacagagca tacagcacag gcaatagagg aaggatacgc aaagagtagg
       61 ttgagtaagt agttaattaa taaattagga ctggtagaga ggttttaggc acaaagtgag
      121 tagaatggta atgaatagta agaaagtgcc ttaaatctca cacctttcct caaataagac
      181 aaaattatga caataatatt aagtatagtt tttatatttg taacattgat aattggattc
      241 agacttttag gagggatgat tcactttaat caaaatacaa ggttacggat ggatgcaacg
      301 attcaagtga aggctgagga ttacgataag gatgaaggta tagaagcttt atcagagttt
      361 tataaattaa ttggacacaa acttcacgag ttaggttatt tagaaataac agctcaagag
      421 ctaagagtcg atgaagacat aaatgttcaa tatgagggag agattcttct tccacgtaat
      481 ggcgaagatg atgacgatga tgacggagaa ttaatagtag acaaaagttt tacattttaa
      541 ataagcagac atgaaattag gaaggctttt atgctcaatg agcatacatg gaattatgag
      601 gagagaggaa atctttagaa aggaaccgac atacccagaa aggctaaaga acgaggagga
      661 caggattaac gcaatagtca gtcaatacag gatgacttcc acatccgtta agccgtatga
      721 aaaggaatat tggggtagct ccgaggaaga acggattaaa gaacaccgta atttatctaa
      781 agaactttta gataagaagg tatcggacta ctattacaga acgaatcttg atgtagaact
      841 cgattacgga ggagggcata aggtaacatt cttagcagac tctattaaaa tagaagatgg
      901 taaattaact ataaaagcaa agtaagatat ggaattagta ggaataatag tttatatgat
      961 ctcagtatgc atatgtttta aagaagtaag caaggtgcag attagagact ttgaagggaa
     1021 agggtacata gtatccatcc tgctaggtat agtcggtata atcccaatgc taaacacgat
     1081 tacagcaact ttaatagtaa tgtataactt ggagatgaat aaagattaaa tatgaacagc
     1141 agtaatttaa aagttggaga ctcttgcgaa gttgtaaagg tacttacagg cggaggatac
     1201 cctaagggta cttttaaggt gggaagggat accgtagcga atttagtcta tgaaagtgat
     1261 aggggtgagg agtcgtacat aacattatat aactctctac taaatggaga ggtaagtatt
     1321 gcaggtatgt caaaattaac gatgtttaga aatgagttaa agttcatagg cagattagta
     1381 gttactaaag taaaataata tatggaagaa ttaagaaagt atattggagg taaagaaaaa
     1441 gcgtatatca ggactagaac tcaatttga
//
LOCUS       DATGSI010000577         1833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_369111, whole genome shotgun sequence.
ACCESSION   DATGSI010000577 DATGSI010000000
VERSION     DATGSI010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369111"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1129
                     /gene="wecB"
                     /locus_tag="VK031_08905"
     CDS             14..1129
                     /gene="wecB"
                     /locus_tag="VK031_08905"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HLS54078.1"
                     /translation="MKVITIIGTRPEGIKLAPVIKELESRQEIESIVCITAQHRQMLD
                     QVLNIFEIIPDYDLDIFKKGQSLTDITVRAIKGLEEVLIKEKPDILLVQGDTTTVFTG
                     ALAGFYHRTKIGHVEAGLRTKNLYSPFPEEANRRLTSTLSNYHFAPTAENKLNLLKEN
                     YPIERIFVTGNTVIDALKYVVKNNYVFETNLLNKLDYKERRIILLTCHRRENIGPPME
                     NTFQAIRDMAKKYQDVEFVFPMHLNPKVRNIANKIFSGIERIHLIEPLSYQEITNLIN
                     KSYFVVTDSGGLQEEAPSLGKPVLVVREETERPEGIRAGTAKLIGTSYESVYKGIDSL
                     LSNKREYEKMSKTTNPYGDGHASKRIVDIIIDEISNR"
     gene            1192..1572
                     /locus_tag="VK031_08910"
     CDS             1192..1572
                     /locus_tag="VK031_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HLS54079.1"
                     /translation="MTRKVYDTDLSVASGPYSQAVDAGDFVFVSGQTARNSKKLESNA
                     ISIKEQTQECFKLLFDILNSANLTPDSVVKVNVFLTDMRYFDEMNEVYKRQFSAPYPA
                     RTTVAVSELPLGADVEIELIARKK"
BASE COUNT          672 a          271 c          350 g          540 t
ORIGIN      
        1 tattttgaaa aagattaaag tgataaccat aattggaaca aggccagagg gaataaagct
       61 ggcaccagtc ataaaagaac tggaaagtag acaagagata gaaagtatag tgtgtataac
      121 tgctcaacat aggcaaatgc tagatcaagt attaaatata tttgaaatta tacctgatta
      181 tgatttggac atatttaaaa agggacaaag cctaacagac ataactgtaa gggccatcaa
      241 ggggttggaa gaagttttaa ttaaggaaaa gcctgacata ctactggttc aaggtgatac
      301 aaccacagta tttaccgggg ccttagctgg attttatcat agaacaaaaa taggccatgt
      361 agaagcagga ttaagaacaa aaaatttata ctctcccttt ccagaagaag ccaatagaag
      421 acttacaagt actttaagta actatcattt tgctccaact gcggaaaata agctaaacct
      481 gttaaaagaa aattatccta tagaaagaat atttgtaact ggaaatacag taatagatgc
      541 actaaaatat gtggttaaga ataactatgt ctttgaaact aacttgttaa ataaactaga
      601 ttacaaagag aggaggataa ttttactaac ctgccacaga agagaaaaca ttgggccgcc
      661 tatggaaaat acttttcagg ctataaggga tatggctaaa aaatatcagg atgtagaatt
      721 tgtttttcct atgcatttaa accctaaggt aagaaatata gccaataaaa ttttctcagg
      781 tatagaaaga attcatttaa tagaaccctt atcttatcaa gaaattacta atctaattaa
      841 taaaagttat tttgttgtta ctgattctgg tggccttcaa gaggaagctc cttctttggg
      901 aaaaccagtc ttggtggtga gagaagaaac ggaaagacct gaaggaatta gggcaggtac
      961 agctaaactt ataggtacca gctatgaatc tgtatataaa ggcatagatt ctttattaag
     1021 taataaaaga gaatatgaga aaatgtcaaa aacaacaaac ccctatggtg atggccatgc
     1081 atccaagaga atagtggaca taataattga tgaaatttca aatagatagt tttacagctt
     1141 gatatctgtg gtatattata gagaaactat attattttag ggggaaaaaa catgactagg
     1201 aaggtgtatg atacagattt atcagtagca tcaggaccct actcacaagc agtagatgct
     1261 ggtgattttg tatttgtatc gggacaaact gcaagaaact ctaaaaaact tgaatctaac
     1321 gcaattagta taaaggaaca aacccaggag tgctttaagc ttttatttga tattttaaat
     1381 tcagctaatt taactccaga ttctgtagta aaggtaaatg tttttttaac ggatatgaga
     1441 tactttgatg agatgaatga agtatataaa aggcagtttt cagcaccata cccggctaga
     1501 actactgttg ccgtaagcga actacctctt ggggcagatg tagaaataga attgatcgca
     1561 aggaaaaaat gacctcaatt aggaggtcat ttgctgtttt tgattatttt ctgtatacaa
     1621 taagcataat cttaattcca atccgtattc ggggtctgtt acactccttc ccaaaatttc
     1681 ctcgcattta tcaatccgat atttaacagt atttctatga atatacattg tgttcgcagt
     1741 atccgtaata ttacaattca aatttagata agtttttagt gtgtgtctta ggtcttgcgt
     1801 agcgctatct gtcggatagg ctagatcctt acg
//
LOCUS       DATGSI010000578         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_607783, whole genome shotgun sequence.
ACCESSION   DATGSI010000578 DATGSI010000000
VERSION     DATGSI010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607783"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="VK031_08915"
     CDS             <1..1563
                     /locus_tag="VK031_08915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLS54080.1"
                     /translation="ELDKNVYRITKFPVKLLDKKYGVGTFIVDVTKEYNYKKKLEEIN
                     SKLEEEQQRLALILDSTAEGIFGIDLEGNCTFFNRSGLRILGYDHQEELLGKNVHWII
                     HHSYEDKRPIPIGDCKIMRTVTTGKSIKVDDEVFWRADGSYFNVEYFVNPQYKDGEIV
                     GAVVTFSDITDRKREEEEITFYSYRDPLTGLYNRRFFEMELIRLDRKENLPISVVIGD
                     VNRLKLINDIFGHTSGDILLKKTAEVLRKGFRTDDIIARLGGDEFAVLLPNTGSVEVR
                     KVIERIKKLLSQESIEDLKISMSIGADTKEIPAQDIYKIMENAEEEMYIEKTIHRKSI
                     NKRAVDSLLETLHRKEPREKKHSENSKRLCERIGRELGLPENEIEILKNAAYYHDIGK
                     IVLEKDIIDKRDKLTTRQWKEVKQHPIVGYRILNSCSKTANIAKYVLYHHENWDGTGY
                     PEGLKGDSIPLYSRIIYLANYYERRISAVRGEVATEEKILSDIKMYAGTKFDPKLVQV
                     FIRVIRRESKGK"
     gene            1781..3154
                     /gene="rlmD"
                     /locus_tag="VK031_08920"
     CDS             1781..3154
                     /gene="rlmD"
                     /locus_tag="VK031_08920"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HLS54081.1"
                     /translation="MARKRKNRLIELEIVDNIFPNKAVGEYDGKRIIVKGAIKGQKVK
                     ALLGRKRKDHIEARVLEVIERSPLEKNIGLEEIDNNGGCIYASLLYEDELNLKEKLVL
                     DLFEREGLTDINYQGIEGSPVVKGYRNKMEYTFGDEEKDGPLILGLHKQGKFYSIVDS
                     DGHYLVDKDFTTIRKKVMHYFRNLGTAFYNKRRHEGFLRHLVIRKALSTGEILLNLVT
                     TSQDKLDWEDFIDFLLNIKNMEGKIVGILHTTNDGLADTVQADKLEILFGRDYIVEEI
                     LGLKFEVSPFSFFQTNTFGAEKLYSMVRDFAGNMDKGLVFDLYSGTGTIAQVMASVAE
                     RVIGIEIVKEAVEKARENAKLNKLNNVEFIAGDVLKAVDKLEEEPDLIVIDPPRDGIH
                     PKAINKIIDFNPDSFVYVSCNPVTLVRDLKVFSERGYRIDKMKLMDMFPRTPHVECIA
                     LIQREIM"
BASE COUNT         1245 a          363 c          691 g          904 t
ORIGIN      
        1 gagctagata aaaatgtata taggataact aaattcccag taaagctttt agataaaaag
       61 tatggagtag gtacttttat agttgatgtt acaaaagaat ataattataa aaagaaatta
      121 gaagaaataa actcaaaatt agaagaagaa cagcaaaggc tagctctaat tttagactct
      181 actgcagaag ggattttcgg aatagatttg gaagggaatt gtaccttttt taatagaagt
      241 gggttacgaa ttttaggata tgaccatcag gaagaattgc taggtaaaaa cgtccattgg
      301 attattcatc atagctatga agataaaaga ccgattccca taggggactg taaaattatg
      361 aggactgtaa caacaggcaa aagtataaag gtagacgatg aagtattttg gagggcagat
      421 ggaagctatt tcaatgttga gtactttgta aaccctcagt ataaggatgg agaaattgta
      481 ggggcagtgg taaccttcag tgatattaca gatagaaaga gggaggaaga agaaataacc
      541 ttctacagct atcgagaccc attaaccggc ctatataata gacggttctt tgaaatggaa
      601 ttaattaggc tagatagaaa ggaaaatcta cctatttcag tggtaattgg agatgtgaat
      661 agattaaaac taattaatga tatatttgga catacatcag gagatattct tcttaaaaaa
      721 acagcagagg ttttaagaaa gggatttaga acagatgata taattgctag gctgggtgga
      781 gatgagtttg cagtcttatt accaaataca ggcagcgtgg aagttagaaa ggtaatagaa
      841 aggattaaaa aacttttatc tcaagaaagt attgaagatc ttaaaataag catgtctata
      901 ggagcagaca ctaaagaaat acctgcccag gatatatata agataatgga aaatgctgag
      961 gaagaaatgt atatagaaaa gactattcac agaaaaagca ttaataaaag agctgtagat
     1021 tctttattag aaacattgca cagaaaggaa cctagagaga agaaacattc tgaaaactca
     1081 aaaaggcttt gcgaaagaat aggtagagag ctaggactgc cggaaaacga gatagagatt
     1141 ttgaaaaatg cagcttacta tcatgatata ggaaaaatag ttttagagaa ggatattata
     1201 gataaaagag ataagttaac tactagacaa tggaaagagg taaaacagca tccaatagta
     1261 ggatatagga tacttaactc ttgttctaaa acagcaaata tagctaaata tgtgctttac
     1321 catcatgaaa actgggatgg aactgggtat cctgaaggat taaaaggaga ctctatccca
     1381 ttatattcta ggataatata tctagcaaac tactatgaaa gaagaattag tgctgttaga
     1441 ggagaggtag ctacagaaga gaaaatttta tcagatataa aaatgtatgc tggtacaaaa
     1501 tttgatccga aattagtcca ggtatttatc cgggtaatta ggagagaatc taaaggtaaa
     1561 tagatgtaga tatttttaat aaagccattg cgtatgcagt ggttttattt tattaacaga
     1621 agaatccagt ttaaatttgt tgttaataat aatctcaatc tttataagct gtcagttgcg
     1681 atttaaattt aattataata attttatata ctaaaacttt catcttaaaa gctagttttt
     1741 tacaataaga ctggtataat aatttggaag gagtgagaag atggcaagaa aaagaaaaaa
     1801 caggctgata gagctggaga tagtagataa tatttttcca aataaagcag taggagaata
     1861 tgacggtaaa aggattatag tgaagggtgc cattaaagga caaaaagtaa aggctctctt
     1921 aggaagaaaa agaaaggatc acatagaagc tagggtcttg gaagtcatag aaaggtcacc
     1981 tttagaaaaa aatatagggc ttgaggagat agataataat ggcggttgta tatatgcttc
     2041 tcttttatat gaagatgaat tgaacttaaa agaaaaacta gtattagatc tatttgaaag
     2101 ggaaggcctt acagatataa attaccaggg tattgaggga agcccagtgg ttaaaggcta
     2161 tagaaacaaa atggaatata cctttggaga tgaagaaaaa gatgggccct taattttggg
     2221 acttcataaa caaggaaagt tctattctat agtagacagt gatggacatt acttagtaga
     2281 taaagacttt accaccatta gaaaaaaagt aatgcattat tttagaaatt taggtacagc
     2341 cttttataat aaaaggaggc atgaaggctt cttaaggcat cttgttatta gaaaggcctt
     2401 gtctactggc gaaatacttt taaatttagt aacaactagc caggacaaat tggattggga
     2461 ggattttata gattttttat taaatattaa aaatatggaa gggaagatag tcggtatact
     2521 tcatactaca aacgatggat tagccgatac tgttcaggcg gataagttag aaattttatt
     2581 tggaagggat tatatagtag aagaaatatt aggcttaaaa tttgaagtat cgcccttttc
     2641 ctttttccaa acaaatactt ttggggcaga aaaattatat tctatggtta gggattttgc
     2701 aggaaatatg gataaagggt tagtgtttga tttgtattct ggtactggaa ccatagcaca
     2761 ggtaatggca tctgtggctg aaagggtaat aggaatagaa atagtaaaag aggcagtgga
     2821 aaaggccagg gaaaatgcta aattaaataa gctaaacaat gtagagttta ttgcaggaga
     2881 tgtattaaag gctgtagaca aattagagga agaaccagat ctaatagtaa tagatccgcc
     2941 aagggatggg atccatccga aagctataaa taagataata gattttaatc ctgatagctt
     3001 tgtttatgta tcttgcaatc cagtaacact agttagagac ttgaaggtat tttcagaaag
     3061 aggatatagg atagataaaa tgaaactgat ggatatgttt cctaggactc ctcatgtgga
     3121 gtgcatagcg ttgatacaaa gagaaattat gtagaaatcc ttagatttaa gctatttcat
     3181 gactatttag ctttccaaga aga
//
LOCUS       DATGSI010000579         4667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_417666, whole genome shotgun sequence.
ACCESSION   DATGSI010000579 DATGSI010000000
VERSION     DATGSI010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417666"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VK031_08925"
     CDS             complement(<1..599)
                     /locus_tag="VK031_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HLS54082.1"
                     /translation="MVHPVKLGKRVRMSYSRIDEVLDLPDLIEVQKSSYNWFLEEGLK
                     EVFDDISPIQDYTGNIILEFVNYHIGDSLKYDEDEARERDTTYSAPLKVKVRLINKET
                     GEVKEQEVFMGDLPLMTEKGTFIINGAERVVVSQLVRSPGVYYAENIDKAGRKLYSAT
                     VIPNRGAWLEYETDSNEIVYVRIDRTRKIPITILLRALG"
     gene            complement(867..1250)
                     /gene="rplL"
                     /locus_tag="VK031_08930"
     CDS             complement(867..1250)
                     /gene="rplL"
                     /locus_tag="VK031_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HLS54083.1"
                     /translation="MANEKVLNLIEEVKGLTVLELSELVKALEEEFGVSAAAPMAVAA
                     APAAGADAPAAEEKTEFDVILEAAGGQKIKVIKAVREITGLGLKDAKDLVDNAPKPIK
                     EGASKDEAEEIKAKLEEVGAEVELK"
     gene            complement(1276..1803)
                     /gene="rplJ"
                     /locus_tag="VK031_08935"
     CDS             complement(1276..1803)
                     /gene="rplJ"
                     /locus_tag="VK031_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HLS54084.1"
                     /translation="MREQTLQAKIQNVEEIKEKIEKAQAVVLVDYRGLNVEELTELRS
                     KYREAGIDYKVYKNTMMRFAFKDSGLEEFNEFLKGPSAIAFGYDDPVQAAKITSEFAK
                     EHGKLEIKAGIVDGEIIDVDGVKSLASLPSREVLIAKALGGINAPIQGFANVLQGTIR
                     SLATVLNAIAEEQGA"
     misc_feature    complement(1822..1951)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1997..2698)
                     /gene="rplA"
                     /locus_tag="VK031_08940"
     CDS             complement(1997..2698)
                     /gene="rplA"
                     /locus_tag="VK031_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HLS54085.1"
                     /translation="MGKRGKKYNESLELIDRHKEYGVNEAIDLVLKTAKAKFDETVDL
                     SVRLGVDPRHADQQVRGAVVLPHGTGRTRRVLVFARGEKVSEAEAAGADFVGGEELVE
                     KIQNEGWLDFDVVVATPDMMGVVGRIGRILGPKGLMPNPKSGTVTFEVKKAVEEIKAG
                     KVEYRVDKSSIVHVPIGKVSFGAEKLKENFLVMMDTLIKAKPVAAKGKYLRTVAISST
                     MGPGIKVNAQKLMED"
     gene            complement(2750..3175)
                     /gene="rplK"
                     /locus_tag="VK031_08945"
     CDS             complement(2750..3175)
                     /gene="rplK"
                     /locus_tag="VK031_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HLS54086.1"
                     /translation="MAKKVIAVVRLQIPAGKATPAPPVGTALGPHGVNIMQFTKEFNA
                     KTADQAGMIIPVVLTVYQDRSFSFITKTPPASVLIKKATGINLGSGEPNKTKVAKITE
                     AQVREIAEMKMPDLNASSVEAAMKMIAGTARSMGVEVVQ"
     gene            complement(3269..3820)
                     /gene="nusG"
                     /locus_tag="VK031_08950"
     CDS             complement(3269..3820)
                     /gene="nusG"
                     /locus_tag="VK031_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HLS54087.1"
                     /translation="MTNDVNDREKRAKDAKWYVVHTYSGHENKVKANIEKMVENRGMR
                     DDIFEVAVPTEEFVDTKTGKPKVKERKLFPGYVLVKMIVNDESWYLVRNTRGVTGFVG
                     PASKPIPLTPEEVQALGVQETIVATVDFEVGDVVKVISGPFENFMGTIDSINMEKGKV
                     KVFVSMFGRETLVELDFNQIEKM"
     gene            complement(3860..4063)
                     /gene="secE"
                     /locus_tag="VK031_08955"
     CDS             complement(3860..4063)
                     /gene="secE"
                     /locus_tag="VK031_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HLS54088.1"
                     /translation="MAARTSKKKGRVSAYFRGVRAEMKKVVWPNRQELVNYTGVVILI
                     SAIISVIVYILDLGIHGILKLFI"
     gene            complement(4092..4241)
                     /gene="rpmG"
                     /locus_tag="VK031_08960"
     CDS             complement(4092..4241)
                     /gene="rpmG"
                     /locus_tag="VK031_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HLS54089.1"
                     /translation="MRERITLACTECKQRNYVSKKNKQNTTERLELKKYCKFCKTHTV
                     HRETR"
     gene            complement(4515..>4667)
                     /gene="tuf"
                     /locus_tag="VK031_08965"
     CDS             complement(4515..>4667)
                     /gene="tuf"
                     /locus_tag="VK031_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269603.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HLS54090.1"
                     /translation="EEGVEMVMPGDNAKFTIELITPVAMEEGLRFAIREGGRTVGAGV
                     VSKILE"
BASE COUNT         1273 a         1098 c          605 g         1691 t
ORIGIN      
        1 atgcctagag ctcttagcaa tatggttatg ggaatctttc tagttctgtc tattcgcaca
       61 tatactattt cattggaatc tgtctcatac tctaaccagg ctcccctatt tgggataaca
      121 gtggcagagt atagctttct acctgcctta tcaatatttt ctgcatagta tactccaggg
      181 gatcttacca attgactgac cacaactctt tcagccccat taatgataaa ggtccctttt
      241 tcagtcatta agggcaaatc acccatgaag acttcctgtt ccttaacctc accagtctcc
      301 ttattgatca accttacctt tacttttaat ggtgctgagt aggtagtatc tctttccctt
      361 gcttcatctt catcatattt taaagaatca ccaatatgat aattgacaaa ttccaaaatt
      421 atattcccag tataatcttg aatgggggaa atatcatcga aaacttcctt tagcccttct
      481 tccaaaaacc aattgtatga tgatttttgg acttcaatca aatcaggcaa atctagcact
      541 tcatcaatgc gagaataact catcctgact cgtttaccta attttacagg atgtaccata
      601 aaaaaattca ccccttatta aactaaaata tccaaaagca taaaatactt ttggtaatac
      661 attccaaatc atatttatta atattgcctt cttatggtct gtttatacaa aaagacaata
      721 tctttccata ttatgcaata agttatacta tcacataaaa ctaccaatgt caattagaaa
      781 atcgggactt aagctatctt gtaccattaa ttcaaagtaa attaaaaagg ctctcaattg
      841 aaagcctttt taaatttcat ccctaattat tttaattcta cttctgctcc tacttcttct
      901 aatttagctt tgatttcttc tgcttcatct tttgaagcac cttctttaat tggttttgga
      961 gcattgtcta ctaaatcttt tgcatccttt aagcctaggc ctgttatctc tcttacagcc
     1021 ttgattactt ttattttttg tccacctgca gcttctaata taacgtcaaa ctcagttttt
     1081 tcttctgctg ctggtgcgtc agcgccagct gctggagctg ctgctactgc cattggagct
     1141 gctgcactta ctccaaactc ctcttctaaa gctttaacta attctgataa ctctaatact
     1201 gttaaaccct taacttcttc tattaaattt aatacttttt catttgccat tttataaaac
     1261 ctccatattt ttattttatg cgccttgctc ttcagctatg gcatttagta cagttgccag
     1321 ggatctaatt gtaccctgta atacattagc aaatccttga attggtgcat taattccacc
     1381 aagtgctttt gcaataagaa cttctcttga tggtaagctg gctaagctct taacaccatc
     1441 tacgtctata atttcaccat ctactattcc agccttaatc tctaatttgc catgttcttt
     1501 agcaaactca gatgtgattt tagctgcctg gactggatca tcatagccaa aagctatggc
     1561 agatggcccc tttaggaatt cattaaattc ttctaagcca gaatccttaa aagcaaatct
     1621 catcatggta tttttatata ccttataatc tatacctgct tctctgtatt tacttcttaa
     1681 ttcggttaat tcctctacat ttaaccctct atagtctact aaaactactg cctgagcttt
     1741 ctcgattttt tcttttattt cctcaacatt ttggattttc gcttgaaggg tctgttctct
     1801 caccacttca cctcctacag gttataaaat aataaaggcc tccacgtaga cacgaagaga
     1861 ccttaatttc acctttaaac attcgatcta cctcggtagg aagtttaaac ctttcggtcc
     1921 ctactgtcta cggtacctat ttaattgact cataaaagta tagcaatttt cttgctgctt
     1981 gtcaacaagt taattgttaa tcttccatta atttttgtgc attgacctta ataccaggac
     2041 ccatagtact tgaaatagct actgtcctta agtattttcc tttagcagcc acaggcttag
     2101 cctttataag tgtatccatc ataactaaga aattttcttt tagcttttct gcaccaaatg
     2161 atacctttcc gattggcaca tgcacgatac ttgatttatc tactctgtat tctaccttac
     2221 cggctttgat ttcttcaaca gcctttttaa cttcaaaggt tacagttcca gattttgggt
     2281 ttggcattag accttttggt ccaagtatcc taccaattct tcctactacc cccatcatat
     2341 ctggagtagc tactaccaca tcaaaatcta gccaaccttc attttggatt ttttctacca
     2401 actcttctcc accaacaaag tctgcacctg ctgcctcagc ttcgcttacc ttttcacctc
     2461 tggcaaatac taaaaccctt ctggttctac ctgttccatg aggtaaaact acagcacctc
     2521 taacttgctg gtcagcatgt cttggatcta cgcccaatct tacagatagg tctacagtct
     2581 catcaaactt tgctttggct gtttttaata ctaaatctat ggcttcatta acgccatatt
     2641 ctttatgtct atcaattagt tctagacttt cattatactt ttttcctcgt tttcccatta
     2701 tgcttcctcc ttgtggtatt aacggttacc ctcccaccat atttattaac tattgtacta
     2761 cttctacacc catgctcctt gcagtacctg ctatcatctt catggcagct tcaacggagc
     2821 tggcatttaa gtcaggcatt ttcatttctg caatttccct tacctgagcc tctgtaattt
     2881 ttgcaacctt tgtcttatta ggctctcctg agcctaggtt tattcctgta gcttttttaa
     2941 tcagcactga tgctggtggt gtttttgtaa taaagctaaa ggatctatct tgatacactg
     3001 taagaactac tggtattatc attccagctt gatctgctgt ttttgcatta aactccttag
     3061 taaattgcat aatatttact ccatgtggtc ctaatgcagt tccaacaggt ggcgcaggtg
     3121 ttgctttacc agcaggtatt tgtaatctta caacagctat aactttctta gccatgcgac
     3181 ttgcacctcc tcatatcttg tggtttagcg ggattaaccc tcccacttgc ttcataaatc
     3241 tatttaaaaa gcataattgc ttaataaatt acattttttc aatttggttg aaatccagtt
     3301 ccaccaaggt ttccctacca aacatggaca caaaaacttt taccttgccc ttttccatat
     3361 ttatactatc tatggttccc ataaagtttt cgaaaggtcc agagattacc ttgaccacat
     3421 ctccaacttc aaaatctaca gtagccacta tagtttcttg aactcctagt gcttgaacct
     3481 cttctggagt caagggaata ggcttacttg ctggccctac aaaacctgta actcccctag
     3541 tatttctaac caagtaccag gactcatcat taacaatcat ttttaccaat acatagcctg
     3601 ggaatagttt tctttctttc accttaggtt taccagtttt tgtatcaaca aattcttctg
     3661 taggtacagc cacttcaaaa atgtcatctc tcatgcctct gttttcaacc attttttcaa
     3721 tattggcctt aaccttattt tcgtgccctg aataagtatg gactacatac catttggcat
     3781 cctttgccct tttttctcta tcattaacat cattagtcat acatttggga tcctttcaga
     3841 tcctcccctc cttttaccac tatataaata gcttcaaaat tccatgtatc ccaagatcta
     3901 gaatataaac aatcacagaa ataatggcac tgattagtat gactacccct gtataattca
     3961 ctaattcttg tctattaggc caaacaactt ttttcatttc tgccctaacg cctctgaagt
     4021 aggccgatac tctacctttt ttcttactag ttcgtgctgc catttatctc acatcctaat
     4081 atataatctt cttaccttgt ttccctatga acggtatggg ttttacaaaa cttacaatac
     4141 tttttcaact ctagtctctc tgttgtattt tgcttattct tcttagaaac atagtttctt
     4201 tgtttgcact cagtacaagc tagtgtgatt ctttcacgca taaaaataca cctcccacca
     4261 tcggcacctc tatatactgt atactgcttc ttaatttcat ctgtatttta aaaaaatatc
     4321 ccaacactaa caagggtact agtataaact accacaaaat aaaatggatg tcaataagtt
     4381 attatatact taaaacacct tatcaatacg gctaataaca aactaatagt ctagtattat
     4441 aaatatctaa acctggtcct tcataatctt ccctattcgt tataaaagac taggccaaag
     4501 acctagtctt ttcattattc taaaatctta gatactactc cagctcctac tgtccttcca
     4561 ccttcacgga tagcaaacct tagtccttct tccatggcta caggtgttat tagctctatg
     4621 gtaaatttag cattgtcacc tggcattacc atttctactc cttcttc
//
LOCUS       DATGSI010000580         1807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_136540, whole genome shotgun sequence.
ACCESSION   DATGSI010000580 DATGSI010000000
VERSION     DATGSI010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136540"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VK031_08970"
     CDS             <1..623
                     /locus_tag="VK031_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54091.1"
                     /translation="RQMVGRGGRSTDEIYKPNFTLIDGGGNIDRFGMWSDELDWKDIF
                     FNGKGDDAPKKKIDLEDVDVCENCGYMYPKRTKSCPECGFAPPAPEVTDELDLSDKVA
                     EPIRRIPPPSARAIISYTKRQNEGKAFAFRVLFGCVVDMFRYYRVDKEQYERSKQSGE
                     LKRKVSDMVRPVYFSIINSTEIKEGANRRLPTIIDMALDKVEEFYS"
     gene            1021..>1807
                     /locus_tag="VK031_08975"
     CDS             1021..>1807
                     /locus_tag="VK031_08975"
                     /inference="COORDINATES: protein motif:HMM:NF020379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BT4734/BF3469 family protein"
                     /protein_id="HLS54092.1"
                     /translation="MKMIVSAFKRVNEPNNPYHRSVLKCLDRIKRGVSKDIILNYRKT
                     GDDKYKLQLPGYCFAGTFSKRSIDGLKSPSGLAILDFDEFKSSSEAVDFKDSVCDLEY
                     VFSAFISPSGKGVKVLVKIPKCNDEEYRLYFESLKEYFNTPYWDDSGKDISRFCFESY
                     DPDIYINQDSKVWTKKEEPEAEDIGVGHDKVIVPLRSENQIINRLQSWFSRKYSMDEG
                     QRNDNVYKFAIALSDFGIPQYTAESHLLQYAQKGFSDEEIKKTI"
BASE COUNT          622 a          254 c          378 g          553 t
ORIGIN      
        1 ttcgtcaaat ggttggacgt ggtggacgtt ctaccgatga aatatataag ccaaacttta
       61 ctttaataga tggtggaggt aatatcgata gatttggcat gtggtcggat gaattagatt
      121 ggaaagatat tttctttaac ggaaaaggag atgatgcgcc aaaaaagaaa atagatttag
      181 aggatgtaga tgtatgcgaa aactgcggtt atatgtaccc aaagcgaact aaatcctgtc
      241 cagagtgtgg atttgctccg ccagcgccag aagtaaccga tgagttagat ttatcagata
      301 aggtagcaga gcctattagg aggatacctc cacctagtgc acgtgcaata ataagttata
      361 cgaaaagaca gaatgaagga aaggcatttg cattccgtgt attgtttgga tgtgttgtag
      421 atatgtttag atattataga gtagataaag agcagtacga gcgatctaaa caatcgggag
      481 agttgaagcg caaggttagt gatatggtaa ggccagttta cttttccata ataaacagta
      541 ccgaaataaa agaaggtgct aatcgtaggt tacccactat tatagatatg gcattagata
      601 aagtagaaga gttttactca taacatatat acatcattga ttatacaacc accttaacgg
      661 gtggtttttt atttcctata gtaaaatgac gtaatgacgg tttcaccaca ttccctatac
      721 tctctctcta gaaataaaac aaggttaatt tatttctagc gttgaaaact ttttctatgt
      781 gtcaatgcgt cacggaatgt gttaagccct ttatatatgc gcgttaccga catgacggac
      841 tttattgtat aacgacatat acaacatggc tacaataaaa cttgtattga tagtattatt
      901 tactatattt gcatacatag gcctggaagc cgaaagcatt tttaattacc cgatgtgatt
      961 cttcgatatt ccaggcccga agttttgcat cgggtatttt ttttaaaaaa atattgcatt
     1021 atgaagatga ttgtgtctgc atttaaaagg gttaatgagc ctaataaccc atatcataga
     1081 agcgtattaa aatgcttaga tagaattaaa agaggcgtat ctaaagatat tatacttaac
     1141 tacagaaaga ctggagatga taagtataaa ctgcaactac ctggatattg ttttgctgga
     1201 acgtttagta aaaggtctat tgatgggtta aagtctccat caggtttggc tatactagat
     1261 tttgacgaat ttaaatcaag tagtgaagct gtagacttca aagattctgt ttgtgattta
     1321 gaatatgttt tttcagcgtt tatttctcct agtggcaaag gagtaaaagt attggttaaa
     1381 ataccaaagt gtaatgatga agaatacagg ttgtattttg aatctctaaa ggagtatttc
     1441 aatacacctt attgggatga ttcgggtaaa gatattagta gattctgttt tgaaagctat
     1501 gaccctgata tttatataaa tcaggactct aaggtatgga ctaagaaaga agagccagaa
     1561 gcagaagata taggagtagg tcatgataag gtaatagtgc cacttcgctc cgaaaaccaa
     1621 ataataaata gacttcaatc atggtttagt agaaagtact ctatggatga aggtcaaaga
     1681 aatgataatg tatataaatt cgctattgca ttaagcgatt ttgggatacc acaatataca
     1741 gcagaaagcc atctattaca atatgcgcaa aagggatttt ctgatgaaga gataaaaaag
     1801 actattg
//
LOCUS       DATGSI010000581         1012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_182694, whole genome shotgun sequence.
ACCESSION   DATGSI010000581 DATGSI010000000
VERSION     DATGSI010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182694"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VK031_08980"
     CDS             complement(<1..548)
                     /locus_tag="VK031_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54093.1"
                     /translation="MNFRPKVHYINSGGSDKNLGGHYNDTIKHLPDEEWICVSDQDAL
                     WFPEFVLKQVEDAINTHGNKYKLMGAYTNRIAAERQRAFPSKFDNMDIFEFRKDAEKL
                     HKDRYNRVKPIKDIAGFFMLFSKELWNRVQFRCNTIACDTFFCQDIKRLGYKIGLIEG
                     IYVYHWYRAQSNDPTHYKEHLI"
     gene            complement(514..>1012)
                     /locus_tag="VK031_08985"
     CDS             complement(514..>1012)
                     /locus_tag="VK031_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54094.1"
                     /translation="FVLMDDDTKIKKKGLGKFLIKKHKETGIEHFTIAGKGAKAVSKR
                     MGCTQWDKGAGVMIFCTRKIIDTVGYMNIEYPSKWGHSHIGWSIRVLKSGFMKPFTGW
                     RVSPDGIENYFYSEDIHGDGLHEQNYSKDEKDKAMVLNKKEFIKETTRVNRYYEFSTK
                     STLHK"
BASE COUNT          288 a          241 c          116 g          367 t
ORIGIN      
        1 catatcaaat gctctttata atgtgtaggg tcattacttt gcgctctata ccaatgatat
       61 acataaatgc cctcaataag cccaattttg taacccaatc tcttaatatc ttgacaaaaa
      121 aaggtatcac aggctattgt attacaccta aactgaaccc tattccacaa ctccttagag
      181 aataacataa aaaatcccgc tatatcctta attggtttga cccggttata tctatcttta
      241 tgtaatttct ctgcatcctt cctaaactcg aatatatcca tattatcgaa cttactaggg
      301 aaagcccttt ggcgttcagc tgctattcta tttgtataag ctcccattaa tttatactta
      361 ttcccatgag tatttatagc atcctccact tgctttagta caaattcagg gaaccataag
      421 gcgtcttggt ctgaaacaca tatccattcc tcatcgggta aatgtttaat cgtgtcatta
      481 taatgacccc ctaggttctt atccgagcca ccgctattta tgtaatgtac ttttggtcga
      541 aaattcataa tatctattta ctcgggtggt ttccttaata aattccttct tatttaatac
      601 catagcctta tctttctcat ccttgctata attctgttca tgcaaaccat ctccatgaat
      661 atcctctgaa taaaagtaat tttcaatacc atccgggcta accctccatc ctgtaaatgg
      721 cttcataaaa ccactcttta atactctaat actccaacct atatgagaat gaccccactt
      781 tgaaggatac tctatattca tataccctac tgtatctatt atcttcctcg tacagaatat
      841 cataactcct gcccctttat cccattgagt acatcccatt ctctttgaca ctgctttagc
      901 tcctttccct gcaatagtaa aatgttctat tcctgtttct ttgtgtttct taattaggaa
      961 tttccctaat cctttctttt ttatctttgt atcatcatcc attaatacaa ag
//
LOCUS       DATGSI010000582         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_561245, whole genome shotgun sequence.
ACCESSION   DATGSI010000582 DATGSI010000000
VERSION     DATGSI010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561245"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..503
                     /gene="rbfA"
                     /locus_tag="VK031_08990"
     CDS             126..503
                     /gene="rbfA"
                     /locus_tag="VK031_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HLS54095.1"
                     /translation="MNIKRINRISEEVRKAVSELLYNGLKDPRINSMITVTKVEVTRD
                     LSYANIYVSVLGDDEDKSNSLAGLESAKGFIRNEIGRKVDLRHVPEPVFYLDESIEDA
                     IRLSKLIDKVKEEDSKLRGSDDD"
     gene            493..1461
                     /locus_tag="VK031_08995"
     CDS             493..1461
                     /locus_tag="VK031_08995"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HLS54096.1"
                     /translation="MMIRQMEEAIRLINKSSNIFIASHINPDGDSLGSSLALALALKE
                     KEKKVHVLKSDIVPKDFAFLPGIHLVKEYAEDMGKIDLFIAVDSSDPDRLGENKSILD
                     LAKNIINIDHHISNTNFGTVNIVDPKAAATGQLIFDLIKEMGIRLNKEMAENIYTAIS
                     TDTGNFVYESVTGQTHRIVAQLIDTGINVGEINQNLYENMTIEKIRLLVNTLSAVKFY
                     QGNQVAIAKVTQEMLKDAGALWEDTEGLVSLIRKIDTVEVACLLKEYEPKEIKASLRS
                     KNYLDVSKVCSKLGGGGHIRAAGCTIYSDIEEAEEKIIAEIKSALR"
     gene            1466..2350
                     /gene="truB"
                     /locus_tag="VK031_09000"
     CDS             1466..2350
                     /gene="truB"
                     /locus_tag="VK031_09000"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HLS54097.1"
                     /translation="MNGIINFFKPKGMTSHGAVNFIRKTLGIKRVGHTGTLDPEATGV
                     LPICIGKATRISQYLLEADKEYIAELTLGHQTDTQDSKGNIIAWSDKTVTEDEIMEVF
                     HSFKNKKSQIPPMYSALKHKGKKLYELAREGKTIERKPRPIKIYHLNVLNIIDGKKIT
                     FHIKCSKGTYVRTICNDIGLALGTYGYMSALTRTAVGPFKLADSVGEEYFQDKSPTDL
                     KDIILPADQGLYNLESIKVDKKHYKRLTNGVLIPISNGDLKINKELKVYCSDKFIGIG
                     RILIKDNEKLLKMDKVII"
BASE COUNT          905 a          320 c          513 g          643 t
ORIGIN      
        1 tgtaagagag gtaagtgcag gttatgaagc aggtataggt cttgaagact ataacgacat
       61 aaaagaagga gatatgctag aggcctatat cctagaagag gtaagaaggt agaggagttt
      121 gtcatatgaa tattaaaaga ataaacagaa tctctgaaga agtaaggaaa gctgtatctg
      181 agcttttata taatggatta aaggatccta ggataaactc aatgataact gtaacgaaag
      241 ttgaggttac tagggattta agctacgcta atatctatgt atcggtccta ggtgacgatg
      301 aggacaaatc caattcactg gcaggtttag aaagcgctaa gggatttata agaaatgaga
      361 taggaagaaa agttgattta agacatgtac cagagccagt tttctacttg gatgaatcta
      421 ttgaggatgc tattaggcta tctaaattaa tagataaggt taaggaagaa gactctaagt
      481 taagaggaag cgatgatgat taggcaaatg gaggaggcaa ttagattaat taataaaagt
      541 agtaacatct ttattgcctc ccacataaac ccagatggcg atagcctagg ctcttcctta
      601 gctttggccc tggctttaaa agaaaaggaa aaaaaagtac atgtcttaaa aagtgacata
      661 gttcctaaag attttgcttt tttaccaggg attcacttgg taaaggaata tgctgaggat
      721 atgggtaaaa tcgacttatt tatagctgta gactccagtg atccagatag gctaggagaa
      781 aacaaatcta tattagattt ggcaaaaaat ataataaata tagatcatca tataagtaat
      841 acaaactttg gaactgtaaa tattgtggat ccaaaggctg cagctacagg tcaactaata
      901 tttgacctga ttaaggaaat gggtattaga cttaataaag aaatggctga aaatatatat
      961 actgccataa gtacagatac aggcaacttt gtctatgaaa gtgtaactgg tcaaacccat
     1021 aggatagtgg cccaacttat agatacaggt ataaatgtgg gggaaattaa ccaaaatctt
     1081 tatgaaaata tgaccattga gaaaattaga ttgttagtta atacattaag tgctgtaaaa
     1141 ttttatcagg gtaatcaagt agcaatagcc aaggtaactc aggagatgct aaaagatgct
     1201 ggagccctat gggaagatac agaaggtctg gtgtcactaa ttaggaaaat agacactgtt
     1261 gaagtagcat gcctgctaaa ggagtatgag ccaaaggaaa ttaaggcaag tttacggagt
     1321 aaaaactacc tagatgtttc taaggtatgt agtaaacttg gtggtggagg acatattagg
     1381 gcagcaggtt gtacaattta ttcagacata gaagaggctg aggaaaaaat cattgcagaa
     1441 ataaaatcag cattaaggtg ataaaatgaa cggtataatt aattttttta agcctaaagg
     1501 gatgacatct catggagctg ttaactttat ccgtaagacc ttaggtatca aaagagtagg
     1561 ccatacgggt actttagatc cggaagctac aggagtattg cctatttgta ttggaaaggc
     1621 aactaggatt tcccaatacc tattagaggc agataaagaa tatatagcag agctaacttt
     1681 gggccatcag acagatactc aagatagtaa aggaaatatt atagcatggt cagataaaac
     1741 agttacagag gatgaaataa tggaagtttt ccattcattt aaaaacaaga aaagtcaaat
     1801 cccgcctatg tattcagctt taaagcataa gggtaaaaaa ctatatgaac tggctaggga
     1861 aggaaaaacc atagaacgga aacctagacc aatcaagatc taccatctaa atgttctcaa
     1921 tataatagat ggcaaaaaaa taacctttca tattaagtgc tcaaaaggca cctatgtaag
     1981 aaccatatgt aatgatattg gcttggcctt aggtacatat ggttatatgt ctgctttaac
     2041 aagaactgct gtaggacctt ttaaactggc tgattctgtt ggggaagaat attttcaaga
     2101 taaatcgcct actgatttaa aggatataat actaccggca gatcagggcc tatataacct
     2161 ggaaagcata aaggtagata aaaagcatta taaaaggctt actaacgggg ttttaatccc
     2221 tatatcaaat ggtgatttaa aaataaacaa ggaacttaag gtctattgct ctgataaatt
     2281 tataggcata ggtaggatac ttataaaaga taatgagaaa ctactaaaaa tggacaaagt
     2341 aataatataa ggtggtaaag atatggaaat tatagatctg g
//
LOCUS       DATGSI010000583         1045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_87270, whole genome shotgun sequence.
ACCESSION   DATGSI010000583 DATGSI010000000
VERSION     DATGSI010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87270"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VK031_09005"
     CDS             complement(<1..281)
                     /locus_tag="VK031_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54098.1"
                     /translation="MKNRPTEKLLEMLTKIKDEKELKRYIKTSSSEKESLKLSKYILE
                     VCNKKDIKKSEIIRNADIYRTYGYEILNGSKLPSRDKLLRICIGNKFSL"
     gene            346..>1045
                     /locus_tag="VK031_09010"
     CDS             346..>1045
                     /locus_tag="VK031_09010"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLS54099.1"
                     /translation="MEIISKNYKISLYEELVELVEDKIYIARSSLDDRIYIKKILAPE
                     NYEIYKELKGLNIDNVPKIYEIIKQDGQVIVIEEYINGKTLEDILNEGKLLTEKEVIG
                     HILSLIEILDKLHCPSTNIIHRDIKPSNIMINNDGILKLIDFDISRIHKESKTTDTEI
                     LGTFGYAAPEQFGFDQSDIRTDIYSLGVTMNEMLTGKLPTEETYVGGLSKIISKCLEI
                     DPDRRYQTTGQLKRA"
BASE COUNT          380 a          139 c          182 g          344 t
ORIGIN      
        1 tttaagctga atttgtttcc tatacagatt ctaagtagct tatccctgct gggaagcttg
       61 ctaccattta aaatttcata accataggtt ctgtaaatat ctgcatttct aataatctca
      121 ctttttttga tgtctttttt attgcaaact tccagtatat atttggaaag cttaaggctt
      181 tccttttcag aactggaagt ttttatatat ctttttaatt ctttctcatc tttaattttg
      241 gtcagcattt ctaaaagttt ttctgtaggc ctatttttca tattatcacc tgataaaaaa
      301 ggattatggt ataattataa cataacttct tggaaggtga aggttatgga gattataagt
      361 aagaattata aaatctcttt atacgaagag ctggtagagc tggtggaaga taaaatttat
      421 atagccagat ctagcctaga tgatagaatc tatattaaaa agattttagc gccggaaaat
      481 tatgaaatat ataaagaatt aaaaggctta aatatagata atgtacctaa aatttatgaa
      541 ataattaagc aagatgggca agtaatagtt atagaggaat atataaatgg aaagacactg
      601 gaagacatat taaatgaagg taaactttta actgaaaaag aggtaatagg tcatatccta
      661 tccctaatag agatattgga taaacttcat tgccctagta caaacataat tcatagggat
      721 attaagccat ctaatattat gataaataat gatgggattc taaaactaat agactttgat
      781 atttctcgaa tacataagga gtctaagact acagatacag aaatattagg cacctttggg
      841 tatgcggcac cagaacaatt tggatttgat caaagtgata taaggactga tatatactct
      901 ttaggagtta ctatgaatga aatgctgaca gggaaattgc ccacagaaga gacgtatgta
      961 ggaggtctta gcaagattat ttctaaatgc ttagaaattg atccagatag aagatatcaa
     1021 acaactggcc agcttaaaag agccc
//
LOCUS       DATGSI010000584         1172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_512082, whole genome shotgun sequence.
ACCESSION   DATGSI010000584 DATGSI010000000
VERSION     DATGSI010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512082"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VK031_09015"
     CDS             complement(<1..411)
                     /locus_tag="VK031_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HLS54100.1"
                     /translation="MKRVKLIINPSSGRQLIEGRIDQLSKLLLDDGYIVGKYFTEKKD
                     DAFHETIRTCQGDWDMIIACGGDGTVNEVAAGIAESENKLPVAILAAGTVNDFANYMD
                     IPSRINDFFQMIKREKIIDIDLGMVNNKYFANVAA"
     gene            complement(525..998)
                     /locus_tag="VK031_09020"
     CDS             complement(525..998)
                     /locus_tag="VK031_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54101.1"
                     /translation="MDSTLTVQDLFKLILFLLGIGVGTYFILVLNKINQILGQAKSLI
                     DTNFEELDLTIKQLPGISSNVNEITQEVNQTIKDITPGVDQLINNVNNISGKVSSITE
                     SVDEVTEKAEETFDKVSESIVDTALTFQANTKTFTDYINIIREVFEIIKNALSKK"
BASE COUNT          332 a          242 c          139 g          459 t
ORIGIN      
        1 tgcagccaca ttggcaaaat atttattatt taccattcct aagtcaatat caattatctt
       61 ttcccttttg atcatttgga agaaatcatt tattctacta ggtatgtcca tataattggc
      121 aaaatcattt acagtacctg ctgccaaaat tgcaactggt agtttatttt cactttcggc
      181 tatacctgca gccacttcat ttacagttcc atctccacca caggctataa tcatatccca
      241 atctccctga caggtcctta tggtctcatg aaatgcatca tctttcttct cagtaaaata
      301 tttccctaca atatatccat catctaatag taatttgctt agttgatcta tcctgccctc
      361 tattaattgt cttccagagg aagggttaat gattaacttt actcttttca tttaatcata
      421 cctttcaaat aataatttaa accttagcat tatattaata gtatatcagg aattaatggg
      481 tttacaatat taaattccca taagaaaagc ccttcaccaa gggcttactt tttgcttaag
      541 gcatttttta ttatttcaaa tacttctctg attatattaa tgtagtcagt aaatgttttt
      601 gtattggctt gaaaagttaa agccgtatct actatacttt cagaaacttt gtcaaaagtc
      661 tcctcagcct tttctgtaac ctcatcaaca gattctgtta tggaggatac cttgcctgat
      721 atgttgttta cattattaat cagttgatca acaccaggag ttatatcctt aatagtttgg
      781 tttacctctt gggtaatttc attaacattg ctactaatac ctggcagttg cttaatggtc
      841 agatccagtt cttcaaaatt tgtatcaatc aaacttttcg cttgccctaa aatttgattg
      901 atcttattta agaccaatat aaaatatgtg cctaccccaa tacctagtag gaataaaatt
      961 agtttaaaaa gatcttgtac agttaaagtt gaatccacaa tcctgccccc ttatcttaag
     1021 tgagtcctct tactttatat cttcttttac atccttagta gtttcttcta ctatctcttc
     1081 tatttccttt gccccctttt ttaagtctct agcaacctta tcctttttat ctttcatctc
     1141 tttccaattt tcttttaatt catccttggt tt
//
LOCUS       DATGSI010000585         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_320681, whole genome shotgun sequence.
ACCESSION   DATGSI010000585 DATGSI010000000
VERSION     DATGSI010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320681"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..737)
                     /locus_tag="VK031_09025"
     CDS             complement(51..737)
                     /locus_tag="VK031_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HLS54102.1"
                     /translation="MELIYRGKTKDVYQLGQGQVLFKFKDELTGQDGVFDPGADTVGL
                     SVEGAGRSALRVSKFFFEKLRDEGIPTHYIDADIDKGMMTAYAAEFFGNGLEVICRYR
                     AVGSFIRRYGMYIEKGQPLDAYVEITLKDDDRQDPLITDEALEMLDILTKEEYKLLVG
                     LTRQISKIIRDELAKKELELYDIKLEFGRIGADKHIALIDEISAGNMRVYKSGEYIEP
                     FRLEEILLNE"
     gene            complement(750..2054)
                     /locus_tag="VK031_09030"
     CDS             complement(750..2054)
                     /locus_tag="VK031_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HLS54103.1"
                     /translation="MDSFFQLKEHNTDVKTEVLAGITTFMTMAYILAVNPNILSDAGM
                     DAGGVFTATALSAAIATLIMALYAKYPFALAPGMGLNAFFAYSVVLGDMGKSWQFALT
                     AVLIEGIIFIALSAVNVREAIFDSIPTSLKNAVSVGIGLFIAFIGLEGAEIIVAGDGT
                     LLGLGDLTSGAPIVAVIGILVTAYLFTKEVKGALLLGIIISTIIGIPLGVTILPENFN
                     SIISLPPSVSEVAFQFVSLEEIFSWEMLVAVFTFLFVDIFDTVGTLAGVASKADMLDE
                     EGKLPRVGKALMADAVGTVAGACLGTSTVTTFVESASGVAEGGRTGLTALSTAGMFIL
                     ALFFAPLFTLIPSAATAPALVIVGLFMMSPITKIDFDDFTEAIPAFLTIVMMPYAYSI
                     AEGIVFGMISYVVLKSVTGKFKDISITMWLVSIAFILKIIFI"
     regulatory      complement(2222..2322)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT          770 a          547 c          353 g          808 t
ORIGIN      
        1 tcttcggaac cctaggtaca ctttcactca tagtaaatta tgagcctgtc ctattcattt
       61 agtaatattt cttctaatct aaacggttct atatattctc ctgatttata tactctcata
      121 tttcctgcag atatttcatc aattaaggct atatgtttat ctgctcctat cctaccaaac
      181 tctaacttaa tatcatataa ttccaattcc ttctttgcca gctcatccct tataatctta
      241 gatatttgcc tggttaagcc tactaataat ttgtattctt ccttggttaa aatatctaac
      301 atttctaagg cctcatctgt aattaaagga tcctgcctgt catcatcttt taatgtgatt
      361 tccacataag catctaaagg ctgtcccttt tctatataca ttccatatct cctaataaag
      421 cttcccactg ccctgtatct acatattacc tctaatccat taccaaaaaa ttcagcagca
      481 taagcagtca tcatcccttt atctatatct gcatctatat aatgggttgg tattccttca
      541 tctcttagct tttcaaagaa aaatttactt acccttaagg ccgatcttcc agccccttct
      601 actgagagac ctacagtgtc tgctccagga tcaaatactc catcctggcc tgtaagctca
      661 tccttaaatt taaacaggac ctgtccctga cctaactgat aaacatcctt ggttttaccc
      721 ctatagatta gctccatcta ttccacctac tatataaaga ttatttttaa tataaatgct
      781 atagaaacta accacatggt gattgaaata tccttaaatt tgcctgtaac tgactttaat
      841 accacataag atatcattcc aaatactatt ccctcagcaa tactataagc atagggcatc
      901 attactatgg ttaaaaaggc tggtattgcc tctgtaaaat catcaaaatc tatcttggta
      961 ataggactca tcataaagag gcctactata actaaagctg gagcagttgc tgctgacggt
     1021 attaaggtaa acagcggtgc aaagaataag gccaatataa acatacctgc tgttgaaagg
     1081 gctgttaatc ctgttcttcc cccttcagct actcctgatg cagattccac aaaggtagta
     1141 acagttgaag ttcctaaaca ggctcctgcc acggtaccta ctgcatcagc cattaaggcc
     1201 tttcctaccc ttggaagttt accttcctca tctaacatat ctgcctttga tgcaactcct
     1261 gccaaggttc caacagtatc aaatatatct acaaatagga aggtaaatac agcaactaac
     1321 atttcccagg aaaatatttc ttctaagctt acaaattgaa aggcaacctc gctaacagaa
     1381 ggtggtaagc taattatact attaaaattt tctggaagta ttgtaacccc taaaggaatt
     1441 cctattatag ttgatataat aattcccaat agaagggcac ctttaacttc ctttgtaaat
     1501 aaataggctg taaccaatat acctattact gctacaatag gagctccact ggttaagtct
     1561 cccagaccaa gtagggttcc atctccggca acaattatct ccgctccctc taagccaata
     1621 aatgcaataa ataggcctat acctacagaa actgcatttt ttaagctagt tggtatagag
     1681 tcaaatatag cctctcttac atttacagct gataaagcaa taaatataat accttcaata
     1741 agtactgcag ttaaagcaaa ttgccaactt ttccccatat cacctaaaac tacagaatag
     1801 gcaaagaatg cattaagtcc cattcctggt gccaaagcaa agggatattt ggcatataag
     1861 gccatgatta aagtagctat agctgccgat aaggctgtgg cggtaaatac tcctcctgca
     1921 tccatacctg catctgacag aatattaggg tttacagcca aaatataagc catagtcata
     1981 aaagtagtaa ttcctgccag cacctcagtt tttacatcag tattgtgctc ctttagttga
     2041 aaaaaactgt ccattaaaga tcctccttat ttttttatcc tataaatatt tctactcata
     2101 taaaaattta agcccagtca ggtaggaaaa gcgaaaccta cccgactggg cttttatccc
     2161 ttcggtgtga tacctattag gtatctgtac cgcttggtcc agcaagaata ctcttcggaa
     2221 ccctaggtac actttcactc atagctaaat gatttacggt catttgtaga tacttcccaa
     2281 ccatattatg ggaattatac gagctttatg aaatatttat atgtacaatt agatattagc
     2341 aatagtcttt atataagtca agctaatttt ttattttttc ggattacttt tttgtgtctg
     2401 ccaatctttt aaaagccttt agaaaagttt ttgttaattt gtgcttattt ttctctacat
     2461 aagcttcaat gtggtgga
//
LOCUS       DATGSI010000586         1046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_463695, whole genome shotgun sequence.
ACCESSION   DATGSI010000586 DATGSI010000000
VERSION     DATGSI010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463695"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VK031_09035"
     CDS             <1..762
                     /locus_tag="VK031_09035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HLS54104.1"
                     /translation="HEFSTIPGVLEDVPEIILNIKGIAAKIHTDEPVTLLIDAEGPKE
                     ITAGDLITGLDVEIVNKDHHIATINEDGKLYMELELVRGRGYVVAEKNKKEGQPVGVI
                     AVDSSFTPVKKVNFNVTNTRVGQITDYDKLELEVWTNGAIGASEATSLGAKILTEHLN
                     LFIGLTEHVNDVEIMVEKEEDEKEKVLEMLVEELDLSVRSYNCLKRAQIDTVEELIQK
                     TEEDMMKVRNLGKKSLEEIQNKLAELGLSLRKSDE"
     gene            786..>1046
                     /gene="rplQ"
                     /locus_tag="VK031_09040"
     CDS             786..>1046
                     /gene="rplQ"
                     /locus_tag="VK031_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HLS54105.1"
                     /translation="MTKLRKLGRPTAHRNAMLRNQVTSLLRNGRIETTYTRAKETQRM
                     AEKMITLGKRGDLHARRQALAYIYEKDVVDKLFSEIAPEYEER"
BASE COUNT          421 a          144 c          228 g          253 t
ORIGIN      
        1 cacgagttct ccacaatacc aggagtattg gaggacgtac ctgaaatcat cttaaatata
       61 aaaggcattg ctgccaaaat acacacagat gagcctgtaa cattattgat agatgctgaa
      121 ggtccaaaag aaatcactgc aggagactta ataactggcc ttgatgtgga aatagtaaac
      181 aaggaccatc atatagcaac tattaatgaa gatgggaagc tatatatgga attagaactg
      241 gttagaggca gaggttacgt agttgctgaa aaaaacaaaa aggaaggcca gcctgtggga
      301 gtaatagctg ttgattcctc ttttacgcca gtaaaaaagg tgaatttcaa tgttactaat
      361 actagagtag gccagattac cgattacgac aaattagaat tagaagtttg gactaatggt
      421 gcaatcggag caagtgaagc tacttcatta ggtgccaaaa tacttactga acatctaaat
      481 ctatttatag gactaactga acatgtaaat gatgtagaaa ttatggtaga aaaagaagaa
      541 gatgaaaaag agaaggttct tgaaatgttg gttgaagagc ttgatctatc tgttagatca
      601 tataactgct taaaaagagc ccaaattgat acagtagaag aattaataca aaaaactgaa
      661 gaagatatga tgaaggtaag aaatttaggt aagaaatccc tagaagaaat tcagaataaa
      721 ctagctgaat taggattaag ccttagaaaa agtgatgaat aatcaagcgt gaaggaggga
      781 ttacaatgac gaaattaagg aaacttggtc gacctacagc ccatagaaat gctatgctga
      841 gaaaccaagt aacttcacta ttgagaaatg gtagaataga aacaacttac actagagcca
      901 aagaaactca gagaatggca gaaaagatga taactttagg taaaagagga gatctacatg
      961 ccagaagaca ggctttagct tatatatatg aaaaggatgt tgtggataaa ttattttcag
     1021 aaatagcacc agagtacgag gaaaga
//
LOCUS       DATGSI010000587         3382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_271742, whole genome shotgun sequence.
ACCESSION   DATGSI010000587 DATGSI010000000
VERSION     DATGSI010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271742"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VK031_09045"
     CDS             complement(<1..980)
                     /locus_tag="VK031_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54106.1"
                     /translation="MLLSILLILKFILFTYLTNVKYNKATIIVVSSLISILILTLIYN
                     SKIKKKNRAALIFYSIISLIIFADAAYYSHFDMLPSILALKQLNQLPAASESVKVVLT
                     WKVNLLVLDVPFLLIFRKRLKDTGLFREETRKTLVSLGILSLLGIFIFTGISGNVAAI
                     KNQELYSHHIRDIKDVIIGSYNVKAKEELLTQEDIIELKTRAKLKYGKHTGIGKGKNL
                     IVIQVEALQDFVIGLEYKGQEITPNLNRFIKDRSSIYYDQYYQLIGSGNTSDAEFVSH
                     NSLYPTLGEPIYNKYTENTFYGLPWLLRDNNYTTWALHGYVKEFWNRDKA"
     gene            complement(1167..2540)
                     /gene="ypeB"
                     /locus_tag="VK031_09050"
     CDS             complement(1167..2540)
                     /gene="ypeB"
                     /locus_tag="VK031_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HLS54107.1"
                     /translation="MDNNRRHWVAPVILGILLIASIAWGYNQYAIRKDLEVTLDNHYQ
                     RLFHDVKEHVEKVQVSLSKALVASTIDRNILLFSQIMNDANFAQDKLAQLPVSHAEIG
                     NTQKFLAQAADYSFYLIQRHLDGEDITDEQRKTLASLQNNSTEFNNELSMLHETMANL
                     DLMNISTSLLGFKRNNSNDKENPIHTSLTNLEAHVEQTPELIYDGPFADQILNKKPQG
                     LPNNTIQEKEAADIAKEFYGGKKDVEIEPFEQGENIDELRMPAHTFHLFPEGQQKELA
                     VYMGVSQKGGKVLWMADPRPVGEPTISVEEAESRALEYLKNKGFDSMEPNYSLQYDGS
                     ILFNFAYKENDIVIYPDLVKVKVAMDTGDIVGFDASAYYMNHHNRDINPPTISLEEAK
                     KRLRPSFQLESARLAIIPKGKDEVLCYELKGKQDEGEFLIYVSAYDGKEEQILQLIQN
                     ENGTLTF"
     gene            complement(2562..3278)
                     /gene="sleB"
                     /locus_tag="VK031_09055"
     CDS             complement(2562..3278)
                     /gene="sleB"
                     /locus_tag="VK031_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HLS54108.1"
                     /translation="MLKRKRKIVIRTIALLVLFLAIFQLVGTKNTVETAHKYDRYFAP
                     RTLYWGVTGDDVRTVQDKLSRWGYYDGQVDGIFGARTYRAVRRFQEKNGLRVDGVVGP
                     ATANALGMPAATGGGQGVNRQSDEYLLARTVHGEARGEPYVGKVAVAAVVLNRTRHPS
                     FPNTIAGVVYQPLAFTAVADGQINLTPDKDSLKAARDALNGWDPTYGCLYYWNPATAT
                     SKWIWSRKVTLKIGKHWFGI"
BASE COUNT          931 a          673 c          547 g         1231 t
ORIGIN      
        1 taggccttat ccctattcca aaactccttt acataaccat gaagggccca ggttgtataa
       61 ttattgtctc ttaataacca aggcaggcca taaaaggtgt tttctgtata tttgttataa
      121 ataggctccc ctaaggttgg atacaaggaa ttgtgggata caaattcagc gtctgatgta
      181 ttaccactac ctattaattg ataatattgg tcataataaa tggaggatct atctttaata
      241 aatctattta aatttggcgt aatttcttga cccttatact ccaggcctat gacaaagtct
      301 tgcaaggcct ccacttgaat tactattaaa tttttccctt tacctatacc tgtatgcttg
      361 ccatatttta gcttggccct tgtttttaat tctattatat cttcctgggt caacaattct
      421 tcttttgcct tcacattgta tgaacctatt attacatcct ttatgtccct aatatggtgg
      481 ctgtataatt cttggttttt tatggcagcc acattgccac ttatgcctgt gaatataaaa
      541 attcctagca aagataaaat tccaagtgaa actaaggtct ttctagtttc ctccctgaaa
      601 agtccagtat cttttaacct ttttctaaag attaataaaa aaggaacatc taacactaaa
      661 agatttactt tccaggtcaa aactaccttt acactttcag aggccgctgg cagttggttt
      721 aattgtttta aggctaaaat tgaaggtaac atatcaaaat gtgagtaata ggctgcatct
      781 gcaaagatta ttaaggaaat tatagaatag aaaattaggg ctgccctatt tttcttcttt
      841 attttactgt tataaataag ggttaatata agaatggaaa ttaaactact gacaactatt
      901 atggtggcct tgttatattt tacatttgta agatatgtaa atagaataaa ttttaaaata
      961 agtagtattg acaataacaa tctctatctc cccattctga aacactttcg ttgatatata
     1021 ccctagttta ttttatttga taaaaatttt caagtaagca tcagtctatt tatccagtcc
     1081 ttttatgaaa aatacggcag ataagcctgc cgtattttgt aaatatatga aatgattttt
     1141 acaaatataa ttaaacttac ttattgttaa aatgttagtg taccattttc attttgtatt
     1201 agttgtaata tttgttcttc tttaccatca taggcactaa cgtaaattaa aaattctcct
     1261 tcatcctgct ttcctttaag ttcataacat aatacttcgt ccttgccctt aggtattatg
     1321 gcaagtcttg ctgactctag ttggaagcta ggccttagtc ttttcttagc ttcttctaag
     1381 cttatggttg gagggtttat atctctatta tggtggttca tataataggc tgaagcatca
     1441 aaacctacta tatctcctgt atccatggca acctttacct taaccagatc tggataaatt
     1501 actatatcat tttccttgta ggcaaagtta aatagaatac ttccatcata ttggagagaa
     1561 taattaggct ccatactatc aaaaccctta ttctttaaat attctaaggc cctgctttct
     1621 gcttcctcaa cagatatggt aggctctcct actggccttg gatctgccat ccaaagaacc
     1681 ttgccacctt tttgagaaac acccatatat acggccagtt ctttctgctg tccctctggg
     1741 aaaaggtgga aggtatgggc tggcattctt aattcatcta tattttctcc ctgttcaaag
     1801 ggctcaattt ctacatcctt tttgccaccg taaaattcct tagctatatc tgcagcttcc
     1861 ttttcctgga tagtattgtt tggcaagcct tggggtttct tgtttaatat ctggtctgca
     1921 aagggtccat catatattaa ttctggtgtt tgctccacat gggcttccag atttgttaaa
     1981 ctggtgtgaa ttggattttc tttatcatta ctattattcc ttttaaatcc aagtagactg
     2041 gttgatatat tcattagatc caagttggcc atggtttcat gaagcattga taattcatta
     2101 ttgaattctg ttgaattgtt ttgtaaagag gctaaggttt tcctttgttc atctgttata
     2161 tcctccccat ctagatgcct ttgaatcaaa taaaagctgt agtctgctgc ctgggctaga
     2221 aatttttgtg tattacctat ttccgcatgg cttactggta gctgggccag tttatcctgg
     2281 gcaaaattgg catcattcat tatctgggaa aacaataaga tgtttctatc tattgtggat
     2341 gctaccaagg ccttagataa gctaacctgt accttttcta cgtgttcttt tacatcatga
     2401 aataatcttt gataatggtt atctagtgtt acttccagat cttttcttat ggcatattgg
     2461 ttatagcccc aggctataga tgctataagt agtatgccta ggattactgg agctacccaa
     2521 tgtcttctat tattatccat aacttcctcc atttctataa tctatattcc aaaccagtgt
     2581 tttcctatct ttagtgttac ctttcttgac caaatccatt tagatgtggc tgttgctgga
     2641 ttccaataat ataggcaacc ataagttgga tcccaaccgt ttaaagcatc cctagccgct
     2701 ttcaatgaat ccttatccgg tgttaagtta atctggccat cagccactgc agtaaaggct
     2761 aagggctgat agactactcc tgctatggta tttgggaagg atggatgtct ggttctgttt
     2821 aatacaactg ctgctacagc taccttacca acatagggtt ctcctctggc ctctccatgg
     2881 actgtcctgg ccaataggta ctcatcagac tgcctattaa ccccttggcc tccgcctgtt
     2941 gctgcaggca ttcctaaagc attggccgta gcagggccta ctacaccatc tactctaaga
     3001 ccattctttt cctggaatct tcttacggcc ctgtatgttc tggccccaaa tattccatct
     3061 acctgaccgt catagtatcc ccatcttgaa agtttatcct gaactgttct tacatcgtca
     3121 ccagtaaccc cccaatacaa ggtacgtggg gcaaaatatc tatcatattt atgtgcagtt
     3181 tccactgtgt ttttggttcc tacaagctga aatatggcta aaaacaaaac tagtaaggca
     3241 atagttctaa taacaatttt tctctttctc ttcaacataa agcctccttt gaatcttttc
     3301 tttatttttt gtaagttctt gtgatttatt catattattt gtaggaatgg attaattatg
     3361 tataagtttt ctaacttctg gc
//
LOCUS       DATGSI010000588         1416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_369927, whole genome shotgun sequence.
ACCESSION   DATGSI010000588 DATGSI010000000
VERSION     DATGSI010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369927"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VK031_09060"
     CDS             complement(<1..233)
                     /locus_tag="VK031_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54109.1"
                     /translation="MDKEPTESYSDETIWEDEALAEAFANRAYKLLPFGFQRFSWRML
                     GYANMVDESNSRGSWGSIGNIIMGNHGSSYSGP"
     gene            complement(382..>1416)
                     /locus_tag="VK031_09065"
     CDS             complement(382..>1416)
                     /locus_tag="VK031_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLS54110.1"
                     /translation="NPDITWEVATNQNIGLDGRFWDGLFSFSIDLFEQRRENILTSRG
                     TEVPIYTGLVLPDENIGVTQNQGIEIELKHENNSLSSDLSYSIAGNVAYAKSKIVDIS
                     EPQDIPEYQKREGNPIGAQLLYQAEGIFTTQQEVDSNPTMPGTQVGDLQYQDINDDGV
                     INSADRIRIDKGPIPEITYGFHANMAYKNFSLYANFAGQTRAWTYIFQNCRTTVNCMR
                     DIVLNAYTPGSMDAKYPIMSQESAPGEGVISAMPSTFWLENASFLRLKTLQLGYALPN
                     NIISQIGLTSARVYINGNNLFTVTPMKWFDPEGDPGGVSGESADQVQYSTGDFYPQTR
                     IFNLGINISF"
BASE COUNT          440 a          287 c          231 g          458 t
ORIGIN      
        1 atggggccag agtaagaaga cccatgattc cccattatta tatttccaat agaaccccaa
       61 gaaccccgag aattagattc atcaaccata ttggcatagc ccaacattcg ccaagaaaag
      121 cgttgaaacc caaaaggtaa taacttgtag gcgcgattag caaatgcttc tgctaaagct
      181 tcatcttccc aaatagtctc atcagaatat gattctgtag gttctttgtc caaaacacta
      241 ctaatatcat tacatgaagg gagatgtgat atgaataaac ccagtaataa tatgcataaa
      301 aacccccgct tccttatctt aggcaaatgt ttattcaaac tcttcatagt tatattttag
      361 agattgtaaa atttatcata tctaaaaaga aatgtttatc ccaagattaa agatccttgt
      421 ttggggataa aaatctccag tagaatactg tacttgatca gcagactcgc cactaacacc
      481 accaggatct ccttcaggat caaaccattt catgggtgta actgtgaata aattgttacc
      541 attaatataa actcttgctg atgtcagacc tatttgggat ataatattat taggcaaagc
      601 gtatcccagt tgtaaagttt ttagccggag aaacgaagca ttttccaacc aaaaagtact
      661 tggcattgca cttattactc cttctcctgg agcagattct tgagacataa tggggtattt
      721 tgcatccata cttccagggg tataagcatt taaaactata tctctcatac aatttacagt
      781 agtcctacag ttctggaaga tataggtcca ggctcttgtc tgccctgcaa agtttgcata
      841 taatgagaag ttcttatagg ccatatttgc atgaaatcca taggtaattt caggtatggg
      901 gcctttatct attctgattc tatctgcaga attaataaca ccatcatcat taatgtcttg
      961 gtattgaaga tcaccaacct gtgtacctgg cattgttgga tttgaatcaa cttcttgctg
     1021 ggttgtaaat ataccttcgg cctgatataa aagttgtgca cctattggat taccttccct
     1081 tttttgatat tcaggtatat cctgaggctc gcttatatcc actattttac tttttgcata
     1141 tgcaacatta cctgctatcg aataagaaag atcacttgac aaggaattat tttcatgttt
     1201 caactctatt tcaattcctt gattttgagt aacacctata ttctcatccg gtagtacaag
     1261 tccggtataa ataggaactt cggttcctct tgatgtcagg atattttctc tcctttgttc
     1321 aaataaatct atgctaaagc taaataaacc atcccaaaat cgaccatcga gaccgatatt
     1381 ctgatttgtt gcaacctccc aagttatatc aggatt
//
LOCUS       DATGSI010000589         1921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_271864, whole genome shotgun sequence.
ACCESSION   DATGSI010000589 DATGSI010000000
VERSION     DATGSI010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271864"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VK031_09070"
     CDS             <1..759
                     /locus_tag="VK031_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HLS54111.1"
                     /translation="SENINNFAERIGINLNKIKSERVLILGGGNIGFYLAEKLEKLGI
                     KATIIEKDKERCEYLAERLDRTLVIYGDGTDMDLLQEEDLESYDTFIGVTGFDELNLL
                     MALVAKQSGVDKTIAKTSRPNYAHIVDRLDVDIALNPINITISNILKYIRGGKVVSVS
                     LLLGGEGEVTEMIISKGSPIIGKPLAKLGLPKGIIIGAIVHNGKVHIPNGNSIIQEGD
                     RIIVFSLASDTPILNKLTRPSKKGGLFNGIWGSR"
     gene            737..>1921
                     /locus_tag="VK031_09075"
     CDS             737..>1921
                     /locus_tag="VK031_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HLS54112.1"
                     /translation="MEYGVVAKVLGYLLMLEAGLMTPALLIALYYKSEDWLALLISIF
                     VTGIIGFILSKAFRYRDNVQIKEGMVIVGLGWILVSLFGSLPFILSGAIPSWIDAFFE
                     SVSGFTTTGSSIVDNIESFPKGVLFWRSFIHWIGGMGILVFTVALLPMLGLGAFQLFR
                     TESPGPRPDRIVPRVRDSARILYKIYSVITIIQITLLVVGGMPFYDSMLHAFGTVGTG
                     GFGIKNNSVGFYDSSYIHLIIASFMTICGLNFSLYYSLIKGKWREVIRDEELRLYLGI
                     ILISIVLIALNIYNSMNYSDFFLALRDSYFQVSSIITTTGYSTVDFDQWPAFSKIILF
                     ILMLIGGCAGSTAGGIKVIRILVLLKAIKAEIIRVLHPRAIIPVKVNDKPVSKDIVTG
                     IAI"
BASE COUNT          592 a          297 c          432 g          600 t
ORIGIN      
        1 agtgaaaaca taaataattt tgcagaaaga attggaatca atttaaacaa gattaaatct
       61 gaaagggttc ttattttagg agggggaaat ataggcttct acttggctga gaagctggaa
      121 aagttaggaa ttaaggcaac cattattgaa aaggataagg aaagatgcga atacttagca
      181 gaaaggttgg accgtacact agtaatctat ggggatggga cggatatgga tctactacag
      241 gaggaggatt tagaatccta tgataccttc ataggggtta caggctttga tgaactgaac
      301 ttattgatgg ccttggtagc aaaacaatct ggtgtagata agaccatagc caaaacaagt
      361 aggcccaatt atgcccacat agtagatagg ttagatgtag acatagcttt aaatcccatt
      421 aatataacca ttagcaatat tttgaaatat ataagagggg gcaaggtggt ttcagtttcc
      481 ttactcctag gaggggaagg agaagttact gagatgatta ttagcaaggg ttcacctata
      541 attgggaaac ctttggctaa gttaggatta cccaagggaa tcatcatagg ggcaatcgta
      601 cacaatggca aggtccatat tcccaatgga aatagtataa ttcaggaagg ggatagaata
      661 atcgtatttt cactggcatc ggatacgccc atcttaaata agctaacccg tccaagtaaa
      721 aaaggaggcc tttttaatgg aatatggggt agtcgctaag gtattaggtt atttgcttat
      781 gctagaagct ggattaatga cacctgctct tttaatagcc ttgtactata agtctgagga
      841 ctggttggcc ttattaataa gtatatttgt tactggtatc attggtttta tcttgagcaa
      901 ggcttttaga tatagggata atgttcaaat aaaggagggt atggtcatag tagggctagg
      961 atggatatta gtatccttgt ttggttccct acctttcata ttatcaggag ctattccctc
     1021 ctggattgat gccttttttg aatccgtatc tggatttaca acaacaggct catcaatagt
     1081 agataatata gaatcttttc ctaaaggagt gctattttgg cgttccttta tccactggat
     1141 tggggggatg gggattctag tctttaccgt agcccttttg cccatgttag ggctaggtgc
     1201 cttccagtta tttagaacag aaagtccagg cccaaggcca gataggattg tgcccagggt
     1261 aagggatagt gcaaggattt tatataaaat ttattcggtt ataactataa tacaaatcac
     1321 cttattggtg gtaggaggaa tgccctttta tgattccatg ctccatgcct ttggaaccgt
     1381 tggtactgga gggtttggga taaaaaacaa tagtgtaggc ttttatgata gtagctatat
     1441 ccacctaatt atagctagct ttatgacaat ctgtgggctc aatttttccc tctactactc
     1501 cctaattaag ggcaagtgga gggaggtaat tagggatgaa gaactaaggc tatatttagg
     1561 tataatactt atttctatag tcctaatagc gttaaacatt tataattcca tgaattatag
     1621 tgattttttc ctagctctaa gggattccta ttttcaagta agctccataa taactactac
     1681 aggatattct actgtagatt ttgatcaatg gcctgccttt agcaagataa tcttatttat
     1741 tcttatgcta ataggtggat gtgctggttc tacagccgga ggaattaagg ttataaggat
     1801 tttagtttta ttaaaggcca ttaaggcaga aattattagg gttttacatc ccagagcaat
     1861 tataccggtt aaggtcaatg ataagcccgt atcaaaagat attgttacag ggatagccat
     1921 c
//
LOCUS       DATGSI010000590         1093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_320992, whole genome shotgun sequence.
ACCESSION   DATGSI010000590 DATGSI010000000
VERSION     DATGSI010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320992"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /gene="cbiD"
                     /locus_tag="VK031_09080"
     CDS             <1..779
                     /gene="cbiD"
                     /locus_tag="VK031_09080"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HLS54113.1"
                     /translation="IKRKGLFGKIGQAAINPVPRQMIEKEIRKVASHGFNILISARKG
                     EEIAKKTFNPNIGIEGGISILGTKGIVYPMSDEAYIKSICMEIDKVEADYGRDHIILV
                     PGNYGRKIAKELQVQGPVVKVSNYIGESLLYIYNKGFKSVTLLGHIGKFSKLSIGIFN
                     THSRICDARLEAFIYYLAFMEADISLLKQVMGALTAEEGLNICIDKGYGQIVALMEKG
                     AEERIKRYIKDEDYKVKVIIYSMERGVDFGNSGRCRSGKS"
     gene            751..>1093
                     /locus_tag="VK031_09085"
     CDS             751..>1093
                     /locus_tag="VK031_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HLS54114.1"
                     /translation="MAGVGPGNPKYISLEVIEKIREFKYVVAFPRIATSLKDIREDII
                     VAKRVEDVLSLIDKQEEILLLASGDPNFYGIVEFLKRKKIKIREILPGLTSFQYMMAK
                     LGKAWQGAHFLS"
BASE COUNT          379 a          159 c          262 g          293 t
ORIGIN      
        1 ggattaaaag aaagggcctg tttggaaaaa taggtcaggc agccatcaat ccagtaccta
       61 gacagatgat tgaaaaggaa ataagaaagg ttgcctccca tggctttaat atacttattt
      121 ccgcccggaa gggagaagaa attgctaaaa aaacctttaa tcctaatatt ggtatagaag
      181 gtggcatatc catcctaggg accaagggaa tagtctatcc tatgagtgat gaagcctata
      241 taaaaagtat atgtatggaa attgataagg tggaggctga ctatggcaga gaccatataa
      301 tcttggttcc aggaaactat ggaaggaaaa tagccaagga gcttcaggtt caaggacctg
      361 tagtaaaggt gtctaactat atcggagaaa gtcttcttta tatatataat aagggattta
      421 aatctgtaac cttactaggg catataggaa agttttccaa actatctata ggaatattta
      481 atacccatag cagaatatgt gatgccaggc tggaggcctt tatatactat ctagccttta
      541 tggaggctga catttcccta ctgaaacagg taatgggggc cttaacagca gaagaaggat
      601 taaatatttg tatagataag ggatatggac agattgtagc ccttatggaa aagggggcag
      661 aagaaaggat taaaagatat ataaaagatg aggactacaa ggtaaaggtc atcatatact
      721 ctatggaaag aggtgttgat tttggtaaca gtggcaggtg taggtccggg aaatcctaaa
      781 tatatttcct tggaggttat agaaaaaata agagaattta aatatgtggt tgcctttccc
      841 agaatagcaa caagcctaaa ggatataagg gaggatataa ttgtcgcaaa aagggtggaa
      901 gatgtattgt ccttgataga taagcaggag gaaatcctac ttctggcatc tggtgatcct
      961 aacttttatg ggattgtaga atttcttaaa aggaagaaga tcaaaatcag ggagatctta
     1021 cctgggctca catcttttca gtatatgatg gctaagttag gaaaggcctg gcaaggggcc
     1081 cactttttat ccc
//
LOCUS       DATGSI010000591         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_463938, whole genome shotgun sequence.
ACCESSION   DATGSI010000591 DATGSI010000000
VERSION     DATGSI010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463938"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VK031_09090"
     CDS             complement(<1..218)
                     /locus_tag="VK031_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54115.1"
                     /translation="MKTKGKLLIIIIIIITLLLMACGSEKEKAEVAKKDMAPNSLQEI
                     HNDLVDILDKVGIIEKLNLGMTIPSEED"
     gene            complement(268..1725)
                     /gene="cls"
                     /locus_tag="VK031_09095"
     CDS             complement(268..1725)
                     /gene="cls"
                     /locus_tag="VK031_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HLS54116.1"
                     /translation="MRFLMENYMIIFSNLIWINMLLAILLIFFERRNPTSTWLWIMVL
                     TFLPGLGFILYLFIGQDLSKQKMFKTKEEEDACFKNLALEQKEQISSNIFSYKDPNFS
                     RYEDTIKMHLMNSDAYFTQDNSVDIYFTGQEKFDALLTSIKNAKSYIHMEYYIFKSDG
                     IGMKIINALCEKAKEGVEVKLLVDGMGGRRFSKRAIGQLREAGGEVAIFFPPFVPWLS
                     IRINYRNHRKICIIDGREAYVGGFNIGDEYLGLSKKFGNWRDTHIRIEGSAVSSLQWR
                     FFLDWRFATKEEIKSCHSHLHQPGKEKHVGIQIVSSGPDSKWPSVKDGYMKMITDARE
                     RVYIETPYFIPDDSIFETLRLAGLSGVDVRVMIPNKPDHLFVYWASMSYIGELLKAGV
                     RFFTYENGFLHSKVLIADDFISSVGTANLDIRSFKLNFEVNAFIYDEGVNLRLSEKFL
                     NDLNFCKEITLEEYKNRSRMVKIKESVSRLLSPIL"
     gene            complement(1742..>2349)
                     /locus_tag="VK031_09100"
     CDS             complement(1742..>2349)
                     /locus_tag="VK031_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HLS54117.1"
                     /translation="LVTIDVSSQYVGPIIEKLGQRKGELLSMTEAVGGYARLEFSIPA
                     RGLIGYRQEFMTDTRGNGIINTVFDGYAPYKGEIPKRKEGSIISFETGESTVYGLHAA
                     QERGVLFIEPGVQVYKGMIIGSSPKGLDVDVNVCRKKQHTNIRAAGSDEALRLSPPRL
                     MSLEEALEFIEEDELIEVTPLNIRLRKKILDMGQRHRLNKK"
BASE COUNT          676 a          502 c          343 g          828 t
ORIGIN      
        1 tcatcttctt ctgagggaat ggtcatccct aggtttaatt tttcaattat acctacctta
       61 tccagtatat ctactagatc attatggatt tcttgaaggg aatttggtgc catatctttt
      121 ttagcaacct ctgccttttc cttttcacta ccacaagcca ttagcaatag ggtaattatt
      181 attattatta ttattaatag tttaccttta gttttcaaat taatatcacc tcatggttat
      241 tgtaaccatg aggccatatt attatcatta taatataggt gatagtaaac gagatactga
      301 ttccttaatc ttgaccattc ttgatctatt tttatattct tccagggtaa tttctttaca
      361 aaagttaaga tcatttaaaa acttttcact taatcttaaa ttgacccctt catcataaat
      421 aaaggcattt acttcaaaat taagtttaaa gcttctaata tctaagttgg cagttcccac
      481 tgatgatata aagtcatcgg caattaaaac cttggagtgt aaaaatccat tttcataggt
      541 aaagaatcta actcccgcct ttaaaagctc gcctatgtag ctcatactgg cccaatagac
      601 aaataggtga tcaggtttat ttggtatcat cactctcaca tctactcctg aaaggcctgc
      661 taaccttaag gtttcgaata tgctatcgtc tggtataaaa tagggcgttt ctatatagac
      721 cctttccctg gcatctgtaa tcattttcat atagccgtcc ttgacactgg gccatttaga
      781 gtctggaccg ctggatacta tttgtatacc tacatgtttt tccttgcctg gctggtgaag
      841 atgggagtgg caagatttga tctcttcttt agttgcaaat ctccagtcta ggaaaaatct
      901 ccattgtaaa gagctgacag cagagccttc tatcctaata tgggtatccc gccagttacc
      961 aaatttcttt gataggccta ggtactcatc tcctatgtta aatccaccca cataggcctc
     1021 cctgccatct ataatgcaga tttttctatg gttccgataa tttatcctaa tacttaacca
     1081 aggtacaaag ggtgggaaaa aaatagccac ttctccccca gcctccctta attgaccgat
     1141 ggctcttttg gaaaatctcc tacctcccat accatctacc aatagcttta cctcaacacc
     1201 ttctttggcc ttttcacaca gggcattgat aatcttcata ccaataccgt cgcttttaaa
     1261 tatataatat tccatatgaa tataactttt tgcatttttt atgctggtta atagggcatc
     1321 aaacttttcc tgtcctgtaa agtatatatc tactgagtta tcttgggtaa aatatgcatc
     1381 actgttcatt aagtgcattt ttatagtatc ttcatatcta gaaaaatttg ggtccttgta
     1441 ggagaaaata ttactactta tttgctcttt ttgctccaaa gctaaatttt tgaaacaagc
     1501 atcttcttct tccttagttt taaacatttt ttgtttggat aaatcttgtc ctatgaataa
     1561 gtataggatg aaacctagac caggtaaaaa tgttaaaacc attatccaca gccaggtgga
     1621 ggtaggattt cgcctctcaa agaatattaa aaggatagcc agtagcatat taatccatat
     1681 caggttgcta aaaataatca tataattttc cataagaaat ctcatttaat cacctatttt
     1741 cttacttttt attaagcctg tgcctttggc ccatgtctag tatctttttt cttagcctta
     1801 tatttaaggg tgtcacttct attaattcat cttcttctat aaattctagg gcctcttcta
     1861 aggacattag cctaggtgga gataatctaa gagcctcatc agacccagca gcccttatat
     1921 tggtatgttg ttttttccta catacattta catctacgtc taagccttta ggactagagc
     1981 ctataatcat acccttataa acctgaactc caggctctat aaatagaact cccctttcct
     2041 gggctgcatg tagcccataa acggtacttt ccccagtctc aaaggaaata atagaacctt
     2101 cctttctttt aggtatctca cccttatatg gggcgtaccc atcaaatact gtattaatta
     2161 ttccgtttcc tctagtatct gtcataaatt cctgcctgta acctattaaa cctctggcag
     2221 gtatagaaaa ctctaacctg gcataaccac ctactgcctc agtcatactt aaaagttcac
     2281 cttttctttg tcctaatttc tcaataatag gtccgacata ctggctggat acatcaattg
     2341 ttaccaatt
//
LOCUS       DATGSI010000592         5869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_654632, whole genome shotgun sequence.
ACCESSION   DATGSI010000592 DATGSI010000000
VERSION     DATGSI010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654632"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /gene="queA"
                     /locus_tag="VK031_09105"
     CDS             <1..960
                     /gene="queA"
                     /locus_tag="VK031_09105"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HLS54118.1"
                     /translation="RSHSKLLVLNREKDTIEHRTFTDIIGYLNSGDTLVLNNTKVLPA
                     RIYGNRIGREEKIEFLLLNSINKDTWESLVRPGKKARVGTKLEFGNGLLKGEVLSIKE
                     DGTRVIKFIYTGIFEEILDRLGEMPLPPYIKARLEDKDRYQTVYSKYKGSAAAPTAGL
                     HFTDSLLADVRKKGVNIAFITLHVGLGTFRPVKVENIKEHHMHSEYFQVTEENARVLN
                     ETRRKNGKVVAVGTTTARTLETIADIDGFVKAQSGWTDIFIYPGYNFKAVDGLLTNFH
                     LPESTLLMLVSAFSTREKILNAYQIAIENQYRFFSFGDAMLII"
     gene            973..2088
                     /gene="tgt"
                     /locus_tag="VK031_09110"
     CDS             973..2088
                     /gene="tgt"
                     /locus_tag="VK031_09110"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HLS54119.1"
                     /translation="MGFKFELIKEAKDCKARLGKIHTPHGTIETPIFMPVGTRATVKT
                     MTPEEVKDLGAQIILANTYHLYLRPGHKLIKEAGGLHDFMNWKHPILTDSGGFQVFSL
                     GDLREIKEEGVEFRSHIDGSKHFISPEKAIEIQNDLGADIIMCFDECTPYPATYEYAK
                     KSMERTLRWAERCKEHHKNGDKQALFGIVQGGMYNDLRKMSAEELIKLDFPGYAIGGL
                     SVGEPKELMCEILDYTTPLLPKDKPRYNMGVGTPDYLFESVIRGIDMADCVFPTRIAR
                     NGTALTSHGRVVVRNAQYAKDFTPLDPECDCYTCTNYSRAYLRHLFNVNEILGARLAT
                     IHNLYFLIKLMENIREAIKEDRLLEYKEEFYKKYGYI"
     gene            2147..2419
                     /gene="yajC"
                     /locus_tag="VK031_09115"
     CDS             2147..2419
                     /gene="yajC"
                     /locus_tag="VK031_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HLS54120.1"
                     /translation="MPQQLQNLILPIGLLAIFYFFAIRPQRKREKEIQEMRSNLKVGD
                     EVVTIGGIYGKIVGVKEDILTLEVGSTKTRLDVTKWSVGSVINKKD"
     gene            2543..2914
                     /locus_tag="VK031_09120"
     CDS             2543..2914
                     /locus_tag="VK031_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HLS54121.1"
                     /translation="MKVRLNLLLILKALCLAILITFGLILIVTFILRFTDLRETKLPM
                     LNNIILILSIVSSSLYLITRTREKGWLYGASLGFLYYLLIIIVNLLFSRFDILQPISL
                     VKLVLAMIIGAIGGIIGINLL"
     gene            3215..4498
                     /gene="secD"
                     /locus_tag="VK031_09125"
     CDS             3215..4498
                     /gene="secD"
                     /locus_tag="VK031_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HLS54122.1"
                     /translation="MKIKDTIILILILAIIGGSTYVAFNGLEVGKIQIKKAKDSIDLG
                     LDLAGGVYVVLEANTDAKGEELAQLMNQTKAIIEERVDGLGISEPSIMVEGDRRIRVE
                     LAGVDDPQEAIDLIGKTAQLQFIEPNGDVVLTGKNVKNAGVAFQKDDLGIEQPVVTLE
                     LDKEGAEKFAQSTGRLIGETNIEDRIIFIVLDDQVISSPAVKHINEGGQPIDDGKAVI
                     SGNFEVEEASYLATLIRAGALPVDLVEQQTSVIGPTLGLEAFEKSIKAGGIALLLIFI
                     FMISIYKLPGLIASIGLIAYTLIIIFTMILMKVKLTLPGIAGLVLSIGMAVDTNILIF
                     ERIRDELLIGKTLRASVDLGFKRALTSVLDSNITTLIAGIVLYYFGVGPIRGFGITLI
                     IGILTSMVTAILLSKYLMKLTLSIFNFKSQKIYGA"
     gene            4513..5376
                     /gene="secF"
                     /locus_tag="VK031_09130"
     CDS             4513..5376
                     /gene="secF"
                     /locus_tag="VK031_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HLS54123.1"
                     /translation="MNIVEKRKIFIGISLSIIILGIIMFIINGFNYGIEFTGGTLIQV
                     NSDEFIEAEKIRPITDEFDENISIVHGGEDKKELILRSTLDLSNADVNKMADDFEKEF
                     GIKKSNFKSEKVGPSMGKEIKNKAVISITIATIAMLIYITFRFEFTFGLAAIIALIHD
                     VLITLSMYAIFKLPVNNAFIAAILTIVGYSINDTIVIFDRIREEIRLNPREELDNIIN
                     SGISHTLRRTINTTITTVIAVSVLYIVGVEDIKVLALPLIFGLLAGTYSSTFIASPLW
                     YIFKNFRSKTA"
     gene            5495..>5869
                     /locus_tag="VK031_09135"
     CDS             5495..>5869
                     /locus_tag="VK031_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HLS54124.1"
                     /translation="MEKWFIKNKGIDYREIARKLNISSIISKILVNRGIYTEDSIDIF
                     LNSSIEKMHPPSQMKDLVKAGDLLKNKIDNGKSIRIVGDYDVDGIMSVYLLYRAFKDL
                     GAKVDYIIPNRVSEGYGLNVEIV"
BASE COUNT         2120 a          776 c         1203 g         1770 t
ORIGIN      
        1 aggtcccatt ctaagttatt ggtactaaat agggagaagg acacaataga acataggacc
       61 tttacagata taataggcta tttgaacagt ggagatacgc tggttcttaa taataccaag
      121 gttttacccg ccagaatata tggaaatagg ataggcaggg aagaaaagat tgaatttcta
      181 ttattaaatt caattaataa ggatacctgg gaaagtcttg taaggccagg taaaaaggct
      241 agagtaggga ccaaactgga atttggaaat ggtctgctaa aaggagaagt cttatcaatt
      301 aaggaagatg gcacaagagt gataaaattt atctatactg gaatatttga agaaatacta
      361 gataggctag gtgaaatgcc tctgccgcct tatataaagg ctagattgga ggataaggac
      421 aggtaccaga cagtctattc aaaatataag gggtctgcag ctgccccaac agcaggactc
      481 cattttactg atagcctatt ggctgatgta aggaagaagg gagtaaacat tgcctttata
      541 accctgcatg tgggcctagg cacttttaga cctgtaaagg tagaaaatat caaagaacat
      601 catatgcact cagaatattt ccaggtaact gaagagaatg ccagagttct taatgaaact
      661 agaagaaaaa atgggaaagt tgtggctgta ggtactacta ctgctaggac tttggagact
      721 atagccgata tagatggctt tgttaaggcc caatcaggat ggacggatat atttatctat
      781 cctggctata attttaaggc agtagatggc ctacttacca acttccacct gccagaatca
      841 accttattaa tgttggtaag tgccttttca acaagagaga agatattaaa tgcttatcag
      901 atagctatag aaaatcaata tagatttttt agctttggcg atgccatgct aataatatga
      961 taggaggaag aaatgggctt taaatttgaa ctaataaagg aagctaaaga ttgtaaggct
     1021 agattaggaa aaattcatac tccccatgga acaatagaaa cacctatatt tatgccagtt
     1081 ggaactaggg ctaccgtaaa gacaatgaca ccagaggaag taaaggattt aggtgctcag
     1141 atcatcctgg ctaatactta ccacctttat ttaaggcctg gtcataaatt aataaaagag
     1201 gcagggggcc tgcatgactt tatgaactgg aaacatccta ttttgacaga tagtggggga
     1261 tttcaagtat ttagccttgg ggatctgagg gaaataaagg aagaaggagt agaatttaga
     1321 tctcatatag atggctcaaa gcactttata agtccggaaa aggctataga gatccaaaat
     1381 gacttagggg ccgacattat tatgtgcttt gatgaatgta ctccctatcc ggctacatat
     1441 gaatatgcca agaaatctat ggaaagaacc ttacgctggg cagaaagatg taaagaacat
     1501 cataaaaatg gggacaaaca ggccttattt ggtatagtgc aaggtggaat gtataatgac
     1561 ttgagaaaaa tgagtgcaga agaactgata aagttagact ttccaggata tgccataggt
     1621 ggcctaagtg taggggagcc aaaggagtta atgtgtgaga tattggatta tacaacaccc
     1681 ttacttccta aagataagcc tagatataat atgggggtag gaactccaga ttatcttttt
     1741 gagtctgtga taaggggaat agatatggca gattgtgttt tcccgaccag gatagctaga
     1801 aatggaacgg ctttaaccag ccatggtaga gttgtagtaa gaaatgccca atatgcaaaa
     1861 gactttacac cattggatcc tgaatgtgat tgctatactt gtactaatta ctctagggct
     1921 tatttgaggc atctatttaa tgtaaatgaa attttaggag ctagactggc taccatccat
     1981 aatttatatt ttttgattaa gctaatggaa aatattaggg aagctataaa agaggatagg
     2041 ctactagaat ataaggaaga attttacaaa aagtatggat atatttaata aataaggatg
     2101 atttatatta gaaatcatgt ataataaata gagaaggagg ggtaaaatgc cacaacaact
     2161 acaaaatcta atattgccaa taggattatt agcaattttt tacttttttg ctattagacc
     2221 tcaaagaaaa agagaaaaag agattcaaga aatgagaagc aatctaaaag taggggatga
     2281 agtagtaacc atcggtggaa tttatggtaa gatagttgga gttaaggaag atatcttgac
     2341 tttagaagta ggttccacca aaaccaggct agatgttact aagtggtcag taggatctgt
     2401 cattaataaa aaggattagt aacttataaa atgaaaatta agattaatcc taatgtagac
     2461 aaaacttcct ttaattaagg aagtttttgt aataaatatc tcttatctac aataaatttt
     2521 aagtagatag gaggtggata ttttgaaagt taggcttaat cttcttctaa ttcttaaagc
     2581 cttgtgtcta gctattctta ttacctttgg tttgatactt atagttactt tcatactaag
     2641 gtttacagat ttaagggaaa caaaattacc tatgttaaat aatataattt taatattaag
     2701 tattgtatct tcatcccttt acttgatcac aagaaccaga gagaagggtt ggctttatgg
     2761 tgctagttta ggttttttat actatctact tattataatt gtgaatttat tattttcaag
     2821 gtttgacata cttcaaccaa tatccctggt aaaactagta cttgctatga ttattggagc
     2881 aattggaggg attataggta ttaacctact atagggagaa gtaaaaacag ggctagacaa
     2941 tggcaaattg cactacaaaa atatttgatt ttcactatta aaaggtagtg ggctccacta
     3001 tcttttatta tttatactct taaaattttt ataacaaaag ctaagattag gttgaaaaaa
     3061 caattaagaa agattattat ataaagagta taactaaaca gatgtaatat tttagggata
     3121 aaattttata gtttcaccta taatctttaa tactgaatag tttaatattc ataaagccaa
     3181 agaacatatt gcataaatag ctaggagggt aaggatgaaa ataaaagaca ctataatttt
     3241 aatactaata ttggctataa taggtggcag tacatatgta gcttttaatg ggctagaagt
     3301 aggaaagata caaattaaga aagctaaaga ttcaattgat ctgggcctag atctggccgg
     3361 tggagtatat gtggttctag aagccaatac tgatgccaag ggagaggaat tggctcaact
     3421 gatgaatcaa accaaggcta ttatagaaga aagggtagat ggattaggta tatctgaacc
     3481 cagcataatg gttgagggag atagaagaat ccgtgttgaa ctggctggcg ttgatgatcc
     3541 gcaggaggca atagatttaa ttgggaaaac agctcaacta cagtttatag aaccaaatgg
     3601 ggatgtagta ttaacaggta agaatgtcaa aaacgcagga gtagcctttc aaaaggatga
     3661 tctggggata gaacagccag tggtaacatt agaattagat aaagaaggag cagaaaagtt
     3721 tgcccaatcc actggtaggt taattgggga aaccaatata gaagatcgga taatatttat
     3781 agtcttagat gatcaggtaa tctccagtcc ggcagtaaag catataaatg aaggtggaca
     3841 gcctatagat gatgggaagg cagttatttc tggtaatttt gaagttgagg aggccagcta
     3901 tttagctacc ctaattaggg caggagcgct gcctgtagat ttagtagagc aacaaacctc
     3961 tgtaatagga cctaccttag gtttagaagc ctttgaaaaa agcataaagg cgggaggaat
     4021 tgccctatta cttatattta tatttatgat atcaatctac aagctaccag gtcttattgc
     4081 ttctataggt ttaatcgcat ataccttaat tataatattt actatgatac ttatgaaggt
     4141 gaaattaacc ttaccaggca ttgcaggctt agttctatct ataggtatgg ctgtagatac
     4201 aaatatatta atatttgaac gaatcaggga tgaattatta ataggtaaaa cccttagagc
     4261 ctctgtagat ctaggattca aaagagcttt aacgtctgtt ttagattcta atataactac
     4321 cttaattgca gggattgttt tatattattt cggagttggg cccattagag gatttggaat
     4381 aaccttaata attggaatct taacatctat ggtaactgca atactacttt ccaaatattt
     4441 aatgaaacta acattatcaa tttttaattt caaaagtcag aaaatatatg gggcttaatg
     4501 gaggtggaga aaatgaatat agtagaaaaa cggaaaatat ttattggaat ttccttaagt
     4561 ataatcatct taggtataat catgttcata attaatggtt ttaattatgg aatagaattt
     4621 acaggaggga ccctgattca ggttaatagt gatgaattta tagaagctga aaagatccgc
     4681 cctataactg atgaatttga cgaaaatata agtatagttc atggtggaga ggacaaaaaa
     4741 gaattaattc taaggagtac cttagacctt tccaatgcag atgtaaataa aatggctgat
     4801 gactttgaaa aagaatttgg tattaaaaag agtaatttta agtctgaaaa agtaggacct
     4861 tctatgggaa aggaaattaa aaacaaggca gtaatttcaa tcactattgc cactattgcc
     4921 atgttaatat atataacctt tagatttgaa tttacctttg gtctggcagc aattattgcc
     4981 ctaattcatg atgtattaat taccttatcc atgtatgcaa ttttcaagct accagtaaat
     5041 aatgccttta ttgcagcaat tttaactatt gtaggttatt ctattaatga taccatagta
     5101 atttttgata ggattagaga agaaataaga ctaaatccga gggaagagtt ggacaatata
     5161 ataaacagtg ggattagtca taccttaaga cggactatta acactacaat aacaacagtt
     5221 attgctgtct ctgtacttta tatagttgga gtggaggata ttaaggtctt agcactacca
     5281 ttaatatttg gtttattggc aggtacctat tcctcaacat ttattgcatc acctttatgg
     5341 tatatattta aaaattttag gtctaaaact gcttaatagg cagtttttct cttatatata
     5401 ttaccaatta aactaaatag attactggaa cgaaactggt ttaaatggta aaatctagat
     5461 acttagagga attgaaacaa gatggagtgg ttatttggaa aaatggttta ttaaaaataa
     5521 gggaatagat tacagagaaa ttgctagaaa acttaatata agtagtatca tctctaagat
     5581 attagtaaat agggggattt atactgaaga ttcaatagac atatttctta atagtagtat
     5641 tgaaaaaatg catcctccaa gtcaaatgaa ggatttagtt aaggcaggag atttattaaa
     5701 aaataaaata gacaatggaa aaagtattag aatagtagga gattatgatg tggatggtat
     5761 aatgtctgtt tacctactat atagggcttt caaggatctg ggagctaaag tagactatat
     5821 aatacctaat agggtatcag aaggatacgg actaaatgta gaaattgtt
//
LOCUS       DATGSI010000593         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_561668, whole genome shotgun sequence.
ACCESSION   DATGSI010000593 DATGSI010000000
VERSION     DATGSI010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561668"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /gene="rnmV"
                     /locus_tag="VK031_09140"
     CDS             <1..500
                     /gene="rnmV"
                     /locus_tag="VK031_09140"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HLS54125.1"
                     /translation="AVKAAVDAEVISTNGFGYKKELIDTLRSLSKRRGIIILTDPDYM
                     GEKIRRDLASKLNNCKHAFLPKGKALKKGDIGVENASKEDIRKAIEGAKPIRVKATKK
                     FSKADMIKYGLSGGPDARSRREKLGEILGIGYANSKQFLNRLNNFSISKEEFLKAMER
                     IKIND"
     gene            493..1359
                     /gene="rsmA"
                     /locus_tag="VK031_09145"
     CDS             493..1359
                     /gene="rsmA"
                     /locus_tag="VK031_09145"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HLS54126.1"
                     /translation="MTKSRLYAPSYVREILKRYGFQFSKGLGQNFLIDGNIVRNIVER
                     ANITENDYVLEIGPGIGTLTEELALRAKKVVSIEIDKDLLPVLDETLSDFNNIEIVHG
                     DILEINLEELIQEKFQDQRIKVVANLPYYITTPIIAKLIEEGLNIQSITVMVQKEVAD
                     RMVAQPGSKAYGSLSVFVNFYTRPEILLRVPKTVFMPQPKIDSAVIKLTSRERLPDVN
                     REQFFALVKAGFSKRRKTILNSLSTYGFDLDREDIRKALEQAGIDPRARAENLSIEDF
                     LNISAILPPLNI"
     gene            1394..2563
                     /locus_tag="VK031_09150"
     CDS             1394..2563
                     /locus_tag="VK031_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54127.1"
                     /translation="MSRGLNYARKFIIFKKEYSNLPDTDPKGHGRLELKGKSLSIYLN
                     IDNAESNNYYDVILIGSDNIHRIGKIYTEENKSGKGEFNLQYRELVEIGFPLERINGI
                     LILRDEKVLLGGYFSQDDGTIEDYIKGLKEREIKEKSDYQEEIELSEEEQDEIDIEPE
                     KVQEKEIEDEVIQEEEIEDKKTERIEQEKAEPNKITKEAYTETMEEVFERDKEIEEDK
                     EKEETINIFKEFPKTFMPEIEEVEVDQTVGYILNILNYFPYVEPFALKLKGYDWWKID
                     VDDPHTDKSFLPYFNYILDKEKEDIIADKSTTAGDLMKEFGHYLFGFYRENRGVRFFV
                     YGVPGDFTIDQHPLKGATGFNTWFEAKDSKGYWLLYIEAATGRIIYPINPMIPEE"
     gene            2582..2944
                     /locus_tag="VK031_09155"
     CDS             2582..2944
                     /locus_tag="VK031_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLS54128.1"
                     /translation="MGKTEDRLTCLAHFAEEDLLLEIKKNLEEEKGIDSLSRIFKAMG
                     DPSRLRIIYALSKSPLCVCDIATVLDMTQSSISHHLRVLRNLNLVKFKREGKLVIYSL
                     DDEHVFRIFKEGLEHTKH"
BASE COUNT         1204 a          390 c          665 g          812 t
ORIGIN      
        1 gggctgtgaa ggctgcagta gatgcagaag ttatttctac caatggcttt ggatataaaa
       61 aggaactgat tgatacctta agaagcctat ctaaaagaag aggcataatt attttaacag
      121 atccagatta tatgggagag aaaataagaa gggacctggc atccaaatta aacaactgta
      181 aacatgcctt tttgccaaag gggaaggcct tgaaaaaggg agatatagga gtagaaaatg
      241 catctaagga agacataaga aaggccatag aaggggctaa acctattagg gtaaaggcta
      301 ctaaaaagtt ttcaaaggca gatatgataa aatatggact gtcaggtgga ccagatgcta
      361 gaagcagaag ggaaaaatta ggggaaatat taggtatagg ctatgccaat tcaaaacaat
      421 ttctaaatag gctaaacaat ttcagtataa gtaaggaaga atttttaaag gctatggaaa
      481 ggattaagat aaatgactaa aagtaggcta tatgctccca gttatgtaag ggagatatta
      541 aaaaggtatg gttttcaatt ttccaagggc ttgggacaaa attttctaat agatggaaat
      601 attgtaagaa atatagtaga aagggccaat attactgaaa atgattatgt cctagaaatt
      661 ggtccaggaa ttgggacctt gacagaagaa ctggccctaa gggccaaaaa ggtagtatca
      721 atagaaatag ataaggatct tttacctgta ttagatgaaa ccttatctga ctttaataat
      781 atagaaattg tccacgggga cattttagaa ataaatctag aggaattaat tcaagaaaag
      841 tttcaagacc aaaggattaa ggtagtggcc aacctgccct attatataac cacccctatt
      901 atagcaaaat tgatagaaga agggctaaat atacaatcaa taactgtaat ggttcaaaaa
      961 gaagtggcag ataggatggt ggcccagcca ggtagtaagg catatggatc cctgtctgtc
     1021 tttgtaaact tttataccag gccggaaata ctattaaggg taccaaaaac agtctttatg
     1081 ccacaaccta aaatagattc ggcagtaatt aaactaacta gcagggaaag gctacctgat
     1141 gtaaacaggg agcaattttt tgccctagtg aaggccggct tttccaagag aagaaaaacc
     1201 attttaaatt ctttatctac ctatgggttt gatttggaca gagaagatat aagaaaggcc
     1261 ttagagcaag cagggataga tcctagagcc agggcagaaa atttaagcat tgaagatttt
     1321 cttaatatta gtgcaatatt acctccttta aacatataaa tgtataaatg tgtttattaa
     1381 ggaggttttt tatatgagca ggggactaaa ctatgctaga aagtttatca tatttaaaaa
     1441 ggaatattct aatttgccag atacagaccc aaaaggacat ggcagattgg agttaaaggg
     1501 gaaaagccta agtatttatt taaacattga taatgcggaa agcaacaact attacgatgt
     1561 tatcctaata ggttcagaca atatccatag aatagggaaa atatatacag aagaaaataa
     1621 aagcggtaag ggagagttta atctacaata cagagaatta gtagaaatag gctttccttt
     1681 agagagaata aatggtattc ttatcttacg agatgaaaaa gttttacttg gaggttactt
     1741 ttctcaagat gacggaacaa tagaagacta tataaagggt ttaaaagaaa gagaaataaa
     1801 agaaaagtca gattatcagg aagagataga gttatcagag gaagaacagg atgaaataga
     1861 tatagagccg gagaaagtac aagagaaaga aatagaagat gaggtaatac aagaggaaga
     1921 gatagaagat aagaaaacag aaagaataga gcaggaaaag gcagagccga ataagataac
     1981 taaagaggct tatactgaaa ctatggagga agtttttgaa agggataagg aaatagaaga
     2041 ggacaaggaa aaagaggaaa caataaatat attcaaagaa tttccaaaga catttatgcc
     2101 tgaaatagaa gaagttgaag tagaccaaac tgtaggttat atattaaata ttttaaatta
     2161 ttttccctat gtggagccat ttgcacttaa attaaaaggg tatgattggt ggaaaataga
     2221 tgttgatgat cctcatacag ataagagctt tctaccatac tttaattata tacttgacaa
     2281 ggaaaaggaa gatattattg ctgataaatc cactacagcc ggcgatttaa tgaaagaatt
     2341 tggacactac ttatttggtt tttataggga aaacagagga gtaagatttt ttgtctacgg
     2401 agttccggga gattttacta tagaccaaca ccctttaaag ggagcaactg gctttaatac
     2461 ctggtttgaa gctaaagatt ccaaaggata ttggctttta tatatagaag cagccactgg
     2521 aaggataata tatccgataa atcctatgat accagaagaa taaacttaag ggaggagaaa
     2581 aatggggaaa acagaagata gattgacttg tctggcccac tttgctgaag aagatttgct
     2641 attggaaata aagaaaaatt tagaagaaga aaaaggtata gattctttgt ctagaatatt
     2701 taaggctatg ggggatccat ctagattaag aataatatat gccctatcta agtcgccact
     2761 gtgtgtatgc gatattgcca cagtattgga catgacccaa tcttccatat cccatcatct
     2821 aagggtatta agaaatttaa atttagtaaa atttaagaga gagggaaagc tagtaatcta
     2881 ttccctggat gatgagcatg tgtttagaat ctttaaagaa gggctagaac atacaaaaca
     2941 ctagtatatc ccaaatgatt aattatagat ttaaagggta ttatagaagt ataataggct
     3001 tgacactgat accctcaggg ggtatactat agataatgtt atattaggag gaataaaaat
     3061 gaaggatgtt g
//
LOCUS       DATGSI010000594         5100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_183320, whole genome shotgun sequence.
ACCESSION   DATGSI010000594 DATGSI010000000
VERSION     DATGSI010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183320"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="VK031_09160"
     CDS             complement(<1..1543)
                     /locus_tag="VK031_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLS54129.1"
                     /translation="MEILGKGIKKMREKYKKKKELHPQSKPSYFTLKENLIYSQNLLK
                     AGSWIYSLSDEKVFWTNEIYNILECRPEELDNVLEAFYDYIHPDDTREVKEAVKRALQ
                     GKEYEIEYRIVTPKGRHKFLLEKIRVIKDSRGEIEKIAGIIKDITESKIIENNNYILG
                     KHINYLRNMSGVGSWKYDYTKGQIYWSEEVYDIYEIGVEELGNKVCNLKKLIHPEDLI
                     ILEKKFNEGLEGRVIDLEHKIILKNGKEKYLLIKGEPIFNKFADVVGLVGVVWDVTKI
                     KNSQQKLDEVEREKGRAQKQFRALVQNSSDIFAIIKPDGMIKYISPAVKEILGYRPEE
                     LTGKFIFNSYDKEGVKRAKEMLEKIVHIHKGTIKRDMKCRTKSGDRVYTELIMSNYLD
                     EPFINGIVVNIRDISRRIETEQKLKHLSNHDSLTQLPNRLYFNKKLADSCKMAAKTST
                     GFTLIMLDIDGFKHVNNALGYKLGDHLIIQVVKRLRKYLGNKFMARYLGDQFAIIIDK
                     LDNRTH"
     gene            complement(1584..3815)
                     /locus_tag="VK031_09165"
     CDS             complement(1584..3815)
                     /locus_tag="VK031_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLS54130.1"
                     /translation="MNLFKTNRKKDDIKGRKEENKQGRTSDKDETRRKYETLFKEVND
                     IVEVINPDGVIKYISPSIKRNLGFVSKDLEGENLFHFFQGEQLAKLRSMVIHVLNNPR
                     ETVTENLQRISKSGETLDFEVRIKNMLDKPWIRGLVITSRNISFRIGLKERLDHTSTQ
                     DDVTMLPNKNYLCQEIQIASEAARKNKSKFILSLLDIDDFQYINHALGYSIGDKLILD
                     IGQRLNNYFKNQGLVCHYSGDQFGILLKDLDAVAKYENILAEIIDLFNEPFIIDQYEI
                     NVTTSVGASIYPDDGEDMDSLFKHASIALFHAKNEGKNRYKLFSEEASISSYKEFELK
                     NDLHKALKNGEFTSYFQPIVNVKNNRILAAEALIRWEHPNWGLVTSDEFISIAEETGF
                     IIDLGRRMLEDVCRHYKEWLDKGLPRIKISLNYSSAQFFEENFLENVEQIIRNYGLDP
                     NFLIIEVEESVLRKCDERIAKNLKKLQDIGVEIAIDDFGRGFLTVSCLESFDIDYFKI
                     TSDFLKDVPSNITNSIIVKSIVNMAHELKTKLVVKGIENWGQLSFLKGLNCYAGQGYI
                     FSKALPSKEFERLLRKRVCKPIRDESWEPEEFVERRKFFRVKFHQFLEATMTILELNY
                     KKVNVGDTLVLIKNMGPGGLCFVSNINLPIEKNLVLQFTTQLVNQEIKVVGSPVWTGE
                     IQGGLYEYGVEFNVDENQRAELIKILNLVQIKMRNNILFAEGRFVSDSLDRYFKAIDK
                     GNK"
     regulatory      4098..4221
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            4375..>5100
                     /locus_tag="VK031_09170"
     CDS             4375..>5100
                     /locus_tag="VK031_09170"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HLS54131.1"
                     /translation="MKKLLSSVLLFSIMAVFLVACSGDGGTGVGKGEIVFADAGWESN
                     KFHNAVAGLIAEEVFGYTWTEVPGSTAVMHEGLIKGEVDVHMEEWTDNIPQYQPDVDA
                     GKLHELSVNFDDNYQGIYVPRYVIEGDPERGIEASAPNLKYVWELKDYPDIFEDDEEK
                     GKGRIYGAIPGWEVDNILERKYEYYGLDENFIYFSPGSDAALQSAIISAYDKGEPIAA
                     YYWEPTWLLGMYDMVLLEDEPYTN"
BASE COUNT         1490 a          978 c          719 g         1913 t
ORIGIN      
        1 tatgagttct attatctagc ttatctatta ttatagcaaa ctggtcacct aaatatctag
       61 ccataaactt attgcctaaa tactttctta gtctttttac cacttgtatt attaaatgat
      121 cgcctagctt ataccctaag gcgttgttta catgtttaaa gccatctata tctagcataa
      181 ttaaggtaaa gccggtggag gttttggctg ccattttaca ggaatcggct agttttttat
      241 taaaatataa tctatttggt agttgtgtca gactatcatg attggataaa tgctttaatt
      301 tctgttctgt ttctatcctt ctgcttatat ctcttatatt gacaacaata ccatttataa
      361 aaggttcatc tagataatta ctcattatta actctgtata aaccctatct cctgatttgg
      421 tcctacattt catatctctt ttaattgttc ccttatgaat atgtacaatt ttttccaaca
      481 tttctttggc ccttttaacc ccttctttat catagctatt aaatatgaat ttaccagtta
      541 actcctctgg cctataacct aatatctctt ttactgcagg gctaatgtat tttatcattc
      601 catctggttt aattatggca aaaatatcac tggagttttg aaccaaggcc ctaaattgtt
      661 tttgtgctct acccttttcc ctttctactt catctaattt ttgctgggaa tttttaattt
      721 tggtcacatc ccaaactacc cccaccaatc ctaccacatc tgcaaactta ttaaatatgg
      781 gttccccctt tattaataaa tacttttcct ttccattttt taatatgatc ttgtgttcta
      841 aatctatgac ccttccttct aaaccttcat taaacttctt ttctaaaatt atcagatctt
      901 caggatggat tagttttttt aagttgcaaa ccttatttcc tagctcttcc acccctatct
      961 cgtatatatc ataaacctct tccgaccagt aaatttgacc ctttgtataa tcatatttcc
     1021 aactacctac ccctgacata tttcttaaat aattgatatg tttacctaag atatagttat
     1081 tattttcaat tatttttgat tcagtaatgt ccttaataat tccagctatt ttttcaattt
     1141 ctccccgact atcctttata acccttatct tttctaatag aaatttatgc cttcccttag
     1201 gtgttacaat ccgatattct atctcatatt ccttgccttg gagggccctt tttaccgcct
     1261 cttttacctc ccttgtgtcg tctggatgaa tataatcata aaaggcctcc aatacattgt
     1321 ctagctcctc aggcctgcat tctaaaatat tgtatatttc attggtccaa aatacctttt
     1381 catctgacaa actgtaaatc caactgcctg cctttaagag attttggcta taaattaaat
     1441 tttccttcaa ggtaaaataa gagggctttg actgtggatg tagttctttc ttctttttgt
     1501 atttttctct catctttttt atgccttttc ctaatatctc catcctacac cagcctttcc
     1561 ttgcctattt gtagccactt aaattatttg ttacctttgt caatagcttt gaaatatcta
     1621 tctaaagaat cagatacaaa cctaccttct gcaaatagaa tattatttct catttttatt
     1681 tgtaccagat ttaatatttt aatcaattct gcccgttgat tttcatctac attaaattca
     1741 acaccatact catataagcc accttgtatt tctccggtcc atacagggct gccaacaacc
     1801 tttatctcct gatttactag ttgggtagta aactgtaata ccaagttttt ctctataggt
     1861 agatttatat ttgatacaaa acacaggcca ccaggcccca tgttttttat caagaccagg
     1921 gtatctccta cattaacctt cttgtaattt aattccaata tggtcatggt tgcctctaaa
     1981 aattggtgga attttaccct gaaaaacttc cttctttcca caaattcctc tggctcccag
     2041 ctttcatccc taattggttt acatactctt ttccttaata atctttcaaa ttcctttgaa
     2101 ggtagggcct tgctaaatat atatccttgt ccagcataac aattaagccc ttttaaaaag
     2161 cttagctggc cccaattttc tatccctttt actaccaatt ttgttttcaa ctcatgggcc
     2221 atatttacta tagatttaac aataatcgaa tttgtaatat tagagggcac atcttttaaa
     2281 aaatcagaag ttattttaaa atagtcaata tcaaaggatt ctaaacaact tacagttaaa
     2341 aaacctcttc caaaatcatc aattgcaatc tctaccccta tatcttggag ttttttcaga
     2401 ttttttgcaa tcctttcatc acatttacgc aggacagact cttccacttc tataattaaa
     2461 aaattaggat ctagtccata attcctaatt atttgttcaa cattttccaa aaaattttct
     2521 tcaaagaact gggcacttga ataatttaag gatatcttta tcctaggtag ccccttatct
     2581 aaccattctt tataatgacg gcaaacatcc tccagcatcc ttctgcctaa gtctataata
     2641 aatccagttt cttctgcaat agatataaac tcatcagagg taaccaatcc ccaatttggg
     2701 tgctcccagc ggattaaagc ctctgccgct aatatcctat tatttttcac atttactata
     2761 ggttgaaagt aggaggtaaa ctctccattt tttaaggcct tatgcagatc atttttaagt
     2821 tcaaattctt tataacttga gatactggct tcttccgaaa aaagcttgta cctgtttttc
     2881 ccctcattct tagcatgaaa aagagcaatg cttgcatgct taaataggct atccatatcc
     2941 tctccatcat caggatatat acttgcccca acactggtag taacatttat ttcatattgg
     3001 tcaattatga aaggttcatt aaatagatca ataatttctg ctaatatatt ttcatacttt
     3061 gcaactgcat ccaaatcttt taataggatc ccaaattggt cacctgaata atgacaaacc
     3121 agcccctggt ttttaaaata gttatttaac ctttgcccta tatctaagat taacttatca
     3181 cctatggaat aacctaaggc atggtttatg tattggaagt catctatatc taatagggat
     3241 agaataaatt tagatttgtt ctttctagct gcctctgaag ctatttgtat ttcttgacaa
     3301 agatagtttt tattaggcaa catagttaca tcatcttgtg tagatgtatg gtctaatctt
     3361 tcttttaagc ctattctaaa ggaaatattt ctagatgtta ttacaagccc cctaatccag
     3421 ggtttgtcca acatgttttt tatccttact tcaaaatcaa gtgtctctcc acttttacta
     3481 atcctttgta aattctcagt aactgtctct ctaggattat ttagtacatg tattaccatg
     3541 gatcttaatt tggccaactg ctccccttgg aaaaaatgaa ataaattttc cccttctaaa
     3601 tccttagaga caaatcctaa attccttttt atcgatggac ttatatattt aataactcca
     3661 tcaggattaa ttacttccac aatatcgtta acttccttaa ataaggtttc atattttctt
     3721 ctagtttcat ccttatccga agtcctaccc tgtttgtttt cctcctttct gcctttaata
     3781 tcgtcttttt tcctatttgt tttaaataaa ttcataaaat ttcctccttt tttctgctct
     3841 taatatgtaa tataaaggag agttagtttg taaaatttag tgatataact taattatacc
     3901 actttttacc ctaaattaag ttaaaatttc gacatattta agcgtttctc gacttgactt
     3961 aaattgaccc aattattgta aataacacaa tgggagcgtt tcttaattta ttaactttag
     4021 taatggctaa attcagccat ttgcaatggt gatttacttg tgctatactc tttagatagt
     4081 aatttaataa ttgaaaatga attgcatcgc ttaattcttt tttaagaagc gggggaacca
     4141 attttggggt gaatcgttat gaagagctga ccctttgctc taacccgaca gctaacctcg
     4201 taggcgttaa agggagtcat tttgtgctaa tttttagact atgagctctc atagtttttt
     4261 ttgtttttca aatttagtat ttaaatgtat ttctcctttt aatattgtaa ttttacgatt
     4321 actaatgcaa tcattaggta aaaatactaa atattataag gaaggtgtgt tattttgaaa
     4381 aaattattaa gtagtgtgtt gctattttct attatggctg tatttttagt tgcctgttca
     4441 ggagatggag gaacaggtgt tggtaaaggc gagattgttt ttgctgatgc tggatgggaa
     4501 agcaataagt ttcataatgc tgtagcaggc ttaatagcag aagaagtttt tggttatact
     4561 tggactgagg ttccaggttc aactgcagtt atgcacgaag gtttaataaa gggtgaagtg
     4621 gatgttcaca tggaggagtg gacagacaat atcccacagt atcaaccaga cgtggatgca
     4681 ggtaagctac atgaattaag tgtaaacttt gatgataatt accaagggat ttatgttcct
     4741 agatatgtta tagaagggga tccagaaaga ggcattgagg cttctgcccc aaacttaaaa
     4801 tatgtgtggg agctaaagga ttatcctgat atatttgaag atgatgaaga aaaaggaaaa
     4861 ggcaggatct atggagctat tccaggctgg gaagtagata atatcctaga gagaaaatat
     4921 gaatattatg gattagatga aaatttcata tactttagcc ctggttcaga tgcagcccta
     4981 cagtctgcta taatatcagc ctatgacaaa ggtgaaccaa tagctgccta ttactgggag
     5041 cctacctggt tattaggtat gtatgatatg gtgcttttgg aagatgaacc atatacaaat
//
LOCUS       DATGSI010000595         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_38206, whole genome shotgun sequence.
ACCESSION   DATGSI010000595 DATGSI010000000
VERSION     DATGSI010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38206"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..726
                     /gene="fsa"
                     /locus_tag="VK031_09175"
     CDS             79..726
                     /gene="fsa"
                     /locus_tag="VK031_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HLS54132.1"
                     /translation="MKLFIDTANTDEIKEINDWGVICGVTTNPSLIAKEGRDFKDVVR
                     EIAQIVDGPISAEVISLDKEGMIREARELAEIHPNIVIKVPMTKDGLKAVKVLTEEGI
                     KTNVTLIFSASQALLAAKAGATYVSPFIGRLDDISNEGLYIIEDIARIFSLHNIGTEI
                     IAASIRNPIHVLDAARAGSDIATIPYKVYMQMLEHPLTDIGIEKFLKDWEGIEVK"
     gene            complement(786..1904)
                     /gene="ald"
                     /locus_tag="VK031_09180"
     CDS             complement(786..1904)
                     /gene="ald"
                     /locus_tag="VK031_09180"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HLS54133.1"
                     /translation="MIIGVPKEIKSQENRVGITPAGVDLLVRHNHKVLVENQAGINAG
                     FSNQDYIEAGAEIINNPTKIWKLSDMIIKVKEPQPSEYKYFREDLILFTYLHLANEPE
                     LTKALLKSNSVALAYETVQLKDKTLPLLAPMSEIAGKLAIQQGAIYLESTNGGKGKLI
                     DGAPGVPPAHVVVVGGGIVGTAAAKRAIGLGARVTVLDIDINRLRYLGEVFMGRVETL
                     YSNHYNMIQAIRSADLVIGAVLVPGAQTPKIITEDMVKEMEPGGVIVDVAIDQGGSVE
                     TIQKPTTHQEPVFIKHGILHYAVANIPASVAHTSTLALTNVSISYVLDIANQGWKQAL
                     INDSALAQGANIIQGKITNKAIAQAHNMDYVSLENILF"
BASE COUNT          646 a          374 c          430 g          648 t
ORIGIN      
        1 caatctttgg atctaaggat gtggcagaaa tgactaggtt atttaaaagc ttataaaatc
       61 atgggggagc gtgaagatat gaagctattt attgatactg caaatacgga tgaaattaaa
      121 gagatcaatg attggggcgt aatctgtgga gtaactacca atcctagctt gatagccaag
      181 gaaggtaggg actttaaaga tgtggtaagg gaaattgccc aaattgtaga tggaccaata
      241 agtgcagagg ttatttcttt agataaggaa gggatgatta gagaagccag agagttagct
      301 gaaattcatc caaatattgt tattaaggtt cctatgacca aggatggcct taaggctgta
      361 aaggttttga cagaggaagg gattaaaact aatgttaccc taattttctc tgccagccag
      421 gcacttttgg cggccaaggc aggtgctaca tatgtaagcc cctttatagg taggctagat
      481 gacattagta atgaaggctt atatattata gaagatatag ctagaatatt tagcctacat
      541 aatataggca cagaaattat tgcagccagc attagaaatc caattcatgt tttggatgca
      601 gccagggcag gctcagatat agctaccatt ccttacaaag tctatatgca aatgttagaa
      661 catcctttga ctgatatagg aattgaaaag tttctaaaag attgggaagg catagaagtg
      721 aaataagata aagaggcaaa ttggaaaaag ggagggttta gaaaactctc ccttttttat
      781 gcctattaaa ataagatatt ctccagtgac acatagtcca tattgtgggc ctgggcgata
      841 gccttatttg tgatcttacc ctgaattata ttggcacctt gtgccagggc ggaatcattt
      901 attaaggctt gtttccagcc ttggttggct atatctaata catagctgat gctgacgttt
      961 gttaaggcta aggtggatgt atgggccaca gaggctggaa tatttgccac cgcatagtgg
     1021 aggatgccat gttttataaa gactggttcc tggtgagtgg taggcttttg aatggtttca
     1081 acagagccac cctgatcaat ggcaacgtca actataactc ctcccggttc catttctttt
     1141 accatatctt ccgttataat cttaggagtt tgtgccccag ggactaagac agcacctatt
     1201 accaggtcag cacttcttat tgcttggatc atattataat ggttagaata aagggtttct
     1261 actctgccca taaatacttc acctaggtaa cgaagtcgat taatatctat atctaaaaca
     1321 gttaccctag ctcccaaacc tatggccctt tttgcagcag cagtacctac tatacctcca
     1381 ccaactacca caacatgagc aggaggtaca cctggagctc catcaataag tttgccctta
     1441 ccaccattgg tactttctaa gtaaattgct ccctgctgga tagcaagctt gcctgcaatt
     1501 tcactcatag gggctaaaag tggcagagtc ttatctttta gttgaacagt ttcataggcc
     1561 agggctactg aatttgattt taataaagcc ttagtaagct ccggttcatt ggctaaatgt
     1621 aagtaggtaa aaaggattag atcctctcta aaatatttat attcggaagg ctgaggttct
     1681 ttcaccttta taatcatatc agataatttc caaatctttg taggattgtt aattatctcc
     1741 gcaccagctt ctatgtaatc ttggtttgag aaaccagcat ttataccggc ctgattttct
     1801 acaagcactt tatgattatg tctaacaagt aagtcaactc ctgcaggggt gatgcctacc
     1861 ctattttcct gacttttaat ttcctttggt actccaatta tcactggtac tcctccttgt
     1921 tatgtaaaaa aataatattg tatttatttt atcacaaact agatctattt tcattacttt
     1981 aaattgggaa agccttacca tttgcttaca gtataaaata cgaataaaat tataaaaaat
     2041 tgtcaaggat tagaatgttg ggaggctaaa tttgttgaaa aatcataaaa gtggtagg
//
LOCUS       DATGSI010000596         8018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_87854, whole genome shotgun sequence.
ACCESSION   DATGSI010000596 DATGSI010000000
VERSION     DATGSI010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87854"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VK031_09185"
     CDS             complement(<1..1274)
                     /locus_tag="VK031_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLS54134.1"
                     /translation="MTKTSETIFNLEGAIEHLSQAIKFKTISYPDYDKINFNIFKDFL
                     EFLKDSYPNIHKECEKKIINKYSPVFHWKGKDESLKPLLLIAHYDVVPVEEATLDEWE
                     VEPFEGVIKDNYIWGRGTLDNKNQLIAAMEAVEYLIGIGFVPNRDIYLAFGFDEEVGG
                     NQGARKIAQYFEDKDIQFEAVIDEGGAIIEDMMEDLDKTAALVGVAEKGTTNIRIGVT
                     GQGGHSSMPPSSTAVEKLAKIIDNVNKNPMPARLIGPVEDMMVTMAPHMKGKSFPLKN
                     IRLLFPIIKKVLSKSPVTNSFIRTTITFTMLEGGDAANVLPQSAWATANLRILQGDSV
                     ESTINHIKKVNPGQDIKIEKLHVSEPSLISDTSSKAYQILGQTIKEIYPDVLIVPYLM
                     SGGTDSRKYYNLCKNIYRFMPINITQKDFESI"
     gene            1464..1994
                     /locus_tag="VK031_09190"
     CDS             1464..1994
                     /locus_tag="VK031_09190"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1887..1889,aa:Sec)
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HLS54135.1"
                     /translation="MEGEISLVYWDVIDSPIGPIYLAAVENWLVYCGSTRVNGIDMHT
                     WLDKHLPGMKHKKERNKVLGKAVGQLEPYFKGESKTLDVPVKLIGTEFRKKVWKALQT
                     IPYGESRTYGEIAKQIGNEKATRAVGQANNKNPISYFVPUHRVIGAGGDLVGFGGGLD
                     AKRWLLDLEGITYRNR"
     gene            2171..2458
                     /locus_tag="VK031_09195"
     CDS             2171..2458
                     /locus_tag="VK031_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein, ric"
                     /protein_id="HLS54136.1"
                     /translation="MKKFNKIVEKNMEKLNLYVPVVERVHGGSHPEFYDVRKLFDKIS
                     EKIGKNETEDLEKEFLQLRKVTNNYSIPEDVCESYEAVYNMLEELDRSYGR"
     gene            2495..4348
                     /locus_tag="VK031_09200"
     CDS             2495..4348
                     /locus_tag="VK031_09200"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010276737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HLS54137.1"
                     /translation="MQMRILRRKNQIAFIGGILIAIAFINKWTVNNMTIFNGALILAS
                     LIGLVPIAIQAYQALRVKVVSIDVLVTIAVLGAFFIKNYEESAIVTFLFLFGAYLELR
                     TLHHTRSAIKELTEMAPESALRLTENGEYEEIEVDDVDEGDLLLVKTGGKVPVDGTVL
                     SGEGHINEASITGEAIPVEKEKGSQVFAGTILENGTIQIRADRVGEDTTFGRIIELVE
                     EAQDSKSEAERFIDKFSKYYTPAVLIISLIVWILSKDIELSITILVLGCPGALVIGVP
                     VSNVAGIGNGARHGVLLKGSEVIHDFSRTDTVIFDKTGTLTIGNPKVSETIYCDNQVD
                     DALSYLASIERESSHPLAKAVLEEIGDVKIFPVENTEVIRGEGIIAQVDNSRVAVGNV
                     SLMERENVKLSDKVKGQISSLEEGGNSLVLTAVDGELKIIMGVRDQIRQGAKKDLERL
                     KSLGIKNLIMLSGDNQGTVNIVSQELGLTEAHGNMLPEDKAQYVKELKRQGRTVAFVG
                     DGVNDSPSLAIADIGIAMGGGTDVAIETSDIVLINSDLGHLPHALGLTKATARNMIQN
                     IVIAVGVVLFLLASVFLSEWMNMSIGMLVHEGSILVVILNGMRLLGYKLRR"
     gene            4401..4628
                     /locus_tag="VK031_09205"
     CDS             4401..4628
                     /locus_tag="VK031_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HLS54138.1"
                     /translation="MKTATIQLETLTCPSCMLKIEGAVKGLEGIEKDTVKVSFNSSRV
                     RLDFDEDKISVDEIEKAITKVGYEVKKSRVR"
     gene            4830..6227
                     /locus_tag="VK031_09210"
     CDS             4830..6227
                     /locus_tag="VK031_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HLS54139.1"
                     /translation="METKENKMGVMPIRQLIISMSLPMMVSMLVQALYNIVDSIFVAQ
                     ISENALTAVSLAFPIQNLMIAIAVGTGVGINSLLSRSLGEKNQKKANDTANNGIILSL
                     FSYSFFLIFGLFFSRAYFKSQTQNIEIINYGVTYLSIVAIGSISIFLQITFERLLQAT
                     GKTFYTMITQGVGAIINIILDPILIFGLLGMPKMGMAGAAVATVLGQVVAAILGIYFN
                     YKVNDDIEIRLKGLRPKLDVIKEIYKVGIPSIIMLSITSVTTYGINNILIKFSSTATA
                     VFGVYFKLQSFVFMPVFGLNNGMIPIIAYNYGARNKDRIIETIKLSLTYALAIMTIGL
                     ILIQIFPRQIFFLFNASENMLNIGVPALRIISLSYIFASINIVLSAVYQAFGNGVLSL
                     INAATRQLIVLLPSAYGLSLLGDVNLIWWSYPIAETVALVLSIVFLKYVYDRMINPME
                     EKEKNMTRIARTDNI"
     gene            complement(6272..6640)
                     /locus_tag="VK031_09215"
     CDS             complement(6272..6640)
                     /locus_tag="VK031_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54140.1"
                     /translation="MEIRKNVIVPLGYGKFIRSDKIIGLEPIEDDRGPGKRTYVYVEG
                     SDKPMIASRTETTILSNMVETPKEIIEATAALELLQDILDDISQIGPMLRKSIKKEAK
                     LDLEKIALKIEEIINEPEED"
     gene            7015..>8018
                     /locus_tag="VK031_09220"
     CDS             7015..>8018
                     /locus_tag="VK031_09220"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HLS54141.1"
                     /translation="MAEKNLEAKIKSLKENIITRFKQEDKQWKKILLTFSVVLVLAIG
                     VYITYQSGFSYQVSFNGEVVGFVKEEETVDEAFSLVEDKMADKYGEKAYFHKEVALER
                     VRGHNKDVVDLEELVDTLSEKIGVLKPASVIVVDGIEEVIVETKQIAEEILEELKKPY
                     IEKDDEDKNVEVLEIGFGQEIEIKSKDVPVESILSKKEALAELERTEEEVEKYQIARG
                     DTAWNISRSLNIGMSRLESANPDKDMEKLQPGDEINLTVPKTVVDVIKVEKHKSTEEI
                     DFKTEEKNDSSIYVGEKKVRQEGKKGKKEIITEVTLTNGIVTERKKVKETIIEEPVNK
                     "
BASE COUNT         2775 a         1136 c         1674 g         2433 t
ORIGIN      
        1 tggattgatt caaaatcttt ttgagttata tttataggca tgaacctata tatgttttta
       61 catagattgt agtattttct tgaatcagtt cctccagaca ttaaataggg gactattaaa
      121 acatcagggt atatttcttt aattgtttgg cctagtattt gataggcctt agaacttgta
      181 tcagatataa gggaaggttc tgatacatga agtttctcaa tctttatatc ctgacctggg
      241 ttaacttttt taatatggtt tatggtagat tccacactat ctccctgcag tattcttaaa
      301 ttggcagtag cccaggcact ttgtggcaat acattggcag catctccacc ctccagcatg
      361 gtaaaagtaa tggtggttcg gatgaaagaa ttggtaactg gagatttgga taagactttt
      421 ttaattattg ggaataaaag tcttatattt tttaaaggga aagacttacc cttcatatga
      481 ggtgccatgg ttaccatcat atcctcaacc ggccctatta accgggctgg cataggattt
      541 ttatttacat tatcaataat tttagccaac ttttccacag ccgtactact tggtggcata
      601 gaagagtgtc ctccctgacc tgttactcca attctaatat tggtagtacc tttttctgca
      661 actcctacta gggcagcagt cttatctaaa tcctccatca tatcctcaat aattgcccca
      721 ccttcatcta tgactgcctc aaactgtata tctttgtctt caaaatactg ggcaatcttt
      781 ctggcaccct gatttccccc aacttcttca tcaaagccaa aggccaggta aatatcccta
      841 ttgggtacaa agccaattcc aattaaatat tctacagcct ccatggcagc aataagctgg
      901 tttttgttat ctaaggttcc cctgccccat atataattat ctttaataac accttcaaag
      961 ggctccactt cccattcatc taaggtagct tcctccacag gaaccacatc atagtgggca
     1021 ataagtaata aaggtttaag gctttcatcc tttcccttcc aatgaaatac tggactatat
     1081 ttatttatta ttttcttctc acattcttta tgaatattag ggtaagagtc ttttagaaat
     1141 tctagaaagt ccttaaatat gttaaaattg attttatcat agtcgggata ggatatggtt
     1201 ttaaatttaa tagcttgaga tagatgttca atagccccct ctaggttaaa tatggtttca
     1261 cttgttttag tcattccaat tcccccttcc gtttcttata tattatagta tcaatttagt
     1321 gtgaaagagt aaataaaaaa atgaagactt taaaaagata ctattctaat ttatttaatc
     1381 cttgaatctt aaatatatta ctgatagaat tttccacata aggtatatga agaagtttat
     1441 atttggaaaa caattaaggg ctggtggaag gagagattag cttggtttac tgggatgtaa
     1501 ttgactcacc tattggacca atatatttgg cagcagtaga aaattggcta gtctattgcg
     1561 gtagcactag ggtaaatgga atagatatgc atacttggct ggacaagcat ttgccaggta
     1621 tgaagcataa aaaggagaga aataaagttc taggtaaggc agtagggcaa ttggaaccct
     1681 attttaaagg agaaagtaag accttagacg tgccagtaaa gttaatagga acagaattta
     1741 gaaaaaaggt atggaaggct ttacagacca taccctatgg cgaaagccgt acctatggag
     1801 aaatagcaaa acaaataggc aatgaaaagg ctacccgggc tgtgggccag gccaataaca
     1861 aaaaccctat atcctatttt gtgccctgac acagggtaat aggagccggt ggcgacctgg
     1921 taggctttgg agggggactg gatgctaaaa ggtggttatt agacctggag ggtataactt
     1981 ataggaatag ataaatactg aaagatcttg gcttaagcca ggatcttttt gtgtattaaa
     2041 tgtatagatg attaggagtt agaaaaacta ataaaaagaa aaagtaaaat tttgattagg
     2101 atcaattttt tttagttaga cccgtgctag acttatatac agaaagaaaa gataagagga
     2161 gtgggataaa atgaaaaaat ttaataaaat agtagaaaaa aatatggaaa agttaaattt
     2221 atatgtacca gtggtagaaa gagttcatgg aggaagtcac ccagagtttt atgatgtccg
     2281 taaattattt gataaaataa gcgaaaaaat tgggaaaaat gaaactgaag acttagagaa
     2341 agaatttcta cagctaagga aggttacaaa taactatagt attccagaag atgtatgtga
     2401 aagctatgaa gctgtttata atatgttgga ggaactagat agatcatatg gaagatagat
     2461 ttgggaaaaa ttttattaaa ggagggatat agttatgcaa atgcgtattt taagaaggaa
     2521 aaatcaaatt gcatttatag gtggcatatt aattgcaatt gcttttataa ataaatggac
     2581 agtaaataat atgacgattt ttaatggggc cttgattctt gcatccctta tagggctggt
     2641 acctatagct atccaggcct atcaggcctt aagagtaaag gtagtcagta ttgatgtatt
     2701 agtaacaatt gcagttttag gggcattttt cattaaaaat tatgaagaat cagccatagt
     2761 tactttctta ttcttgtttg gggcatattt ggaattaaga actctccacc atactaggtc
     2821 agccataaag gagttaacag agatggcccc agaaagcgca ttaagactaa cagaaaatgg
     2881 agagtatgaa gaaattgaag tggacgatgt ggatgaagga gatctacttt tggtaaagac
     2941 tggaggaaag gtgcctgtag atggaactgt tttaagtgga gagggccata ttaatgaggc
     3001 cagtattaca ggcgaggcaa ttccagttga aaaggaaaaa ggctctcagg tctttgcagg
     3061 tacaatttta gaaaatggaa ctatccaaat tagggcagac cgggttggag aagatactac
     3121 ctttggaaga attattgagc tggtagaaga agctcaagat tcaaaatctg aagcagagcg
     3181 ctttatagac aaattctcaa aatactatac acctgcagtt cttattatat ctttaatagt
     3241 ttggatcttg tccaaggata tagaattgtc cataaccata ttggttcttg gttgtccagg
     3301 tgctctggtt attggtgtac cagtatcaaa tgtggctggt ataggaaatg gggccaggca
     3361 tggggtgctt ttaaagggta gtgaggttat tcatgatttt agtcggactg atactgtaat
     3421 ctttgacaaa acaggtaccc taaccatagg aaatccaaag gtgtctgaaa ccatatattg
     3481 tgataatcag gtggatgatg ccctatctta tctggccagc atagaaaggg agtctagcca
     3541 tcccttagcc aaggctgtgt tagaagaaat aggggatgtg aaaattttcc ctgtagaaaa
     3601 cacagaggta atcagaggag aaggtattat agcccaggta gataatagca gggtggcagt
     3661 aggaaatgta agcttgatgg aaagggaaaa tgtcaagctt agtgacaagg ttaaagggca
     3721 gataagctcc ctggaagagg gtggaaactc tttagtattg actgctgtag atggagaatt
     3781 aaagataata atgggagtcc gcgatcagat cagacaaggg gctaagaaag atttagaaag
     3841 gctaaaaagc ttaggtatta aaaatttaat tatgctatca ggagataatc aaggtactgt
     3901 caatatagtc agccaagaac taggactaac ggaggcccat ggcaatatgt tgcctgagga
     3961 taaggcccaa tatgttaagg aattaaaaag acaaggtagg actgtggctt ttgtagggga
     4021 cggggttaac gatagtccat ccttagccat agctgatata gggatagcca tgggtggagg
     4081 aacagatgtg gctattgaaa cttcagatat agttttaata aattcagacc taggacatct
     4141 accccacgcc ttagggttga caaaggctac agcaagaaat atgatacaaa atattgttat
     4201 tgcagtaggt gtagtattat ttttactggc cagtgtgttt ttaagtgagt ggatgaatat
     4261 gtctattggt atgctggttc atgagggcag tattttggta gttattttaa atggtatgag
     4321 attattaggt tataaattaa gaagatagat ggactttgat ataatctatc tataataaaa
     4381 agatgaaagg ggcaaatgaa atgaaaacag ctactataca attagaaact ctaacatgtc
     4441 cttcatgcat gttaaagatt gaaggggcag ttaaaggatt ggaaggtata gaaaaggaca
     4501 cagtgaaagt atcctttaac tccagtaggg taagattaga ttttgatgag gataagattt
     4561 cagttgatga aatagaaaag gccataacca aggttggcta tgaagttaaa aaatctaggg
     4621 ttaggtaatt atttagcagg cttcctattt atttaagata agtgggaagc ctatttttgt
     4681 atgcttatat aagtacgttt actaggaagg taaattgggt aaaaaaatag ggaatgtgca
     4741 aaccagtaaa tttgagacta gactttttcc caaaaaaggt atacaatata aatttgtgac
     4801 taaatttata atactaggga gggtgagata tggaaacaaa agaaaataaa atgggtgtaa
     4861 tgcctattag acaacttata ataagtatgt ccctgcccat gatggtatca atgttggtgc
     4921 aggcgttata taatatagta gatagtatat ttgtggctca aataagtgaa aatgcattaa
     4981 ctgcggtttc cttagccttt cctatacaaa atttgatgat agccattgca gtaggaacag
     5041 gtgtcgggat taattcatta ctttccagat cactaggaga aaagaatcag aagaaggcta
     5101 atgacactgc taacaatgga attatattaa gtttatttag ctatagcttt tttctaatat
     5161 ttggattgtt cttctccaga gcctatttta agtctcaaac ccagaatatt gagataatta
     5221 attatggagt aacctattta agtatagtgg ccatcggttc tattagtata tttttacaga
     5281 taacctttga aagattacta caggctacag gaaaaacatt ttatacaatg atcacccaag
     5341 gtgttggggc tattattaat ataatcctgg atcctatttt aatctttggg ctgttaggta
     5401 tgcctaaaat gggtatggct ggtgcagcag tggcaactgt attgggacaa gttgtggctg
     5461 ccatacttgg tatttacttc aactataagg taaatgatga tattgaaata agattaaaag
     5521 gattaaggcc taaactagat gtgatcaaag aaatctacaa ggtaggtatt ccatctataa
     5581 ttatgctatc tattacatct gttacaacat atggaatcaa caatattttg attaagttct
     5641 cttccacagc tacagcagtg tttggagttt attttaaact acaaagcttt gtttttatgc
     5701 cagtatttgg attaaataat ggaatgatcc cgattattgc ttataattat ggagcaagaa
     5761 ataaggatag gattattgaa actataaagt taagtctaac ttatgccttg gccataatga
     5821 ctattgggct tatattaatt caaatattcc ctagacaaat tttcttcttg tttaatgcat
     5881 cagaaaatat gttaaatata ggggtaccgg ctttaagaat aattagctta agttatatat
     5941 ttgccagcat aaatattgta ttaagtgcag tttaccaggc ctttggtaat ggagtcttaa
     6001 gcctgataaa tgctgcaaca aggcagttaa ttgtactttt accttctgca tatggactat
     6061 ctttattggg agatgtaaac ctgatttggt ggtcttatcc tatagcagag actgtggctt
     6121 tggtattaag cattgtattt ttaaaatatg tatatgatag gatgattaat cctatggaag
     6181 agaaggaaaa aaatatgacc aggattgcta gaactgataa tatataaaag gtagaattcc
     6241 aaggtggatt ctaccttttt tgtagaaaga tttaatcttc ttctggttca ttaattattt
     6301 cttcgatttt tagggcgatt ttttccaaat ccaatttagc ttcttttttg atactctttc
     6361 ttaacatggg accgatttga ctaatatcat cgagaatatc ttgtaatagt tccagggcag
     6421 cagtggcctc aataatttcc ttgggagttt cgaccatgtt agataggatg gtggtttctg
     6481 tcctggaggc aatcataggc ttatctgatc cttctacata aacataagtc cttttacctg
     6541 gccccctatc atcttctata ggttccaagc caataatctt atctgatcta ataaacttcc
     6601 cataccctag agggacaata acattttttc taatctccat accttcactt cctttttaaa
     6661 tacacaacta ttagtgttac tatctattat aattataccc tagcttgatt ataccaacat
     6721 ttatagaagg aaaatatatg aagtaattta aaataaaagt cttaatctga caactacttt
     6781 aatacagctt agaaaaattt aatcaaaaat actataacca agggatgaaa ctaggtagat
     6841 attaaggatt caaaaggtaa aaatgctaaa aagatctaag agttgttaaa tccttgtgat
     6901 tttaccaaac tttaattgga aaaagattaa aaaaacattg tcaaatggtt acaataatgt
     6961 tacaatacat aaataagaag ataaatcact ttagatttag ggggatgaac aggagtggcc
     7021 gaaaaaaatt tagaggcaaa aataaagtct ctaaaggaaa atatcataac aaggtttaag
     7081 caggaagaca agcaatggaa aaaaatctta cttacctttt ccgtagtttt ggttttagcc
     7141 attggagttt atattacata tcaaagcggt ttttcttatc aagtaagctt caatggagaa
     7201 gtagtaggat ttgttaagga agaggagact gtagatgaag cctttagctt agtggaagat
     7261 aaaatggcag ataagtatgg agaaaaggcc tatttccaca aggaagttgc attagaaagg
     7321 gtaagaggac acaataaaga tgtggtagac ttggaagaat tggtagatac tctttcagaa
     7381 aaaattggag ttttaaagcc agcgtcagtt attgtggtag acggcataga agaggttatt
     7441 gtagaaacca aacaaatagc agaggaaata ttagaagaat tgaaaaagcc ttacattgaa
     7501 aaagatgatg aagataaaaa tgtggaagta ttggaaatag ggtttggaca agagatagaa
     7561 attaaatcta aggatgtacc tgttgaaagc atactctcta aaaaggaagc cttagcagaa
     7621 ttggaaagaa cagaagaaga agtcgaaaaa tatcagatag caaggggaga cacggcttgg
     7681 aatatttcca gatccttaaa catagggatg agtaggctgg aaagtgctaa cccggacaag
     7741 gatatggaga agcttcaacc aggcgatgag ataaacttaa cagtgccaaa gacagttgtt
     7801 gatgttatta aagttgaaaa acataaaagt actgaggaaa tagattttaa gacagaggaa
     7861 aaaaatgatt catccatata tgtaggagag aaaaaggtta ggcaggaagg taaaaaaggt
     7921 aaaaaggaaa taattactga agtcacccta actaatggta tagtaacaga aagaaaaaag
     7981 gtaaaggaaa ctattataga agagcctgta aacaagat
//
LOCUS       DATGSI010000597         5787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_137378, whole genome shotgun sequence.
ACCESSION   DATGSI010000597 DATGSI010000000
VERSION     DATGSI010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137378"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VK031_09225"
     CDS             complement(<1..260)
                     /locus_tag="VK031_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HLS54142.1"
                     /translation="MNLVTNNIRLITGNAHRELAEKIAKSLNVEMAKCEVTYFMDGEV
                     NVNIGETVRGMDVYIIQPTCTPVNDNLMETLILIDAVKRASAG"
     gene            complement(283..1668)
                     /gene="glmU"
                     /locus_tag="VK031_09230"
     CDS             complement(283..1668)
                     /gene="glmU"
                     /locus_tag="VK031_09230"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HLS54143.1"
                     /translation="MDIAIVLAAGEGTRMRSKRPKVLHRICGMTLLDYVLDASIGAKI
                     EKNIVITGHGGEKVKKHFKDKPIIFKDQPIGEDVPYGTGFAVMQAEDMIEDDSTVVIL
                     CGDTPLITADTLKKFVAYHKEGQYHGTVLTALLDEPAGYGRIKRDKKGNISKIVEDKD
                     ASEEEKEINEINSGIFCFNGRLLKGALKKLDDNNAQNELYITDVVEILNREGYKIGGY
                     IIEDSIEIYGVNSRNQLAFCEKVMRERINKAHMEKGVTLIDPSATYIEPKVIIGADTT
                     IYPGVVLEGNTSIGRDCVIRTGTRIVDSKIEDGVVVESSLIEASKVGQGSNIGPNAHL
                     RPDSRLGKNVKIGNFVEVKNATIGDNTKAGHLAYVGDAIVGRNVNIGCGVIFANYDGV
                     NKHVVTVGDNAFVGSNSNLVAPLKVNDYAYIAAGSTITKEVEKGALAIERGTQVNKEG
                     WVEKRGLKKNN"
     gene            complement(1796..2059)
                     /gene="spoVG"
                     /locus_tag="VK031_09235"
     CDS             complement(1796..2059)
                     /gene="spoVG"
                     /locus_tag="VK031_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HLS54144.1"
                     /translation="MNITDVRVRKITEEGKMKAIVSVTFDDEFVVHDIKIIDGQNGLF
                     IAMPSRKMADGEFRDIAHPINATTRQKIQDAVFAEYEKALATE"
     gene            2319..3734
                     /gene="murC"
                     /locus_tag="VK031_09240"
     CDS             2319..3734
                     /gene="murC"
                     /locus_tag="VK031_09240"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HLS54145.1"
                     /translation="MFNFDLNNKEYNHIHFIGIGGISMSGLAEILLNKGYKVTGTDLK
                     DNPILKRLEKLGATIYIGHSKNNINGADLIIYTDAISTDNEELQAARQSGIPTVDRAM
                     FLGALMKNYTYSIAVSGTHGKTTTTSMIASITNHGTLDPTILLGGELDDIGGNVKLGS
                     QDYILTEACEYKGNILKYFPTTAIILNIDEDHLDYFRDIDHIVDTFAGYAANLEEDSY
                     LLINGDDPHAPKIIESTKAQVVKFGISHKAHYRAENISFSKEGYPSFTLNINNKEFWP
                     INLKVMGKHNIYNALASIAAAHIHGVSMEDITDYIELYNGVHRRLEVKGLVKDIKIMD
                     DYAHHPTEIKATLDALRDVPKNKLYCIFQPHTFTRTKALLDKFASSFMNADQIIITDI
                     YAARENDNGEIHSKDLVQAINDKGADAIYLSTFEEVKKYLMLNIEAGDIVLTMGAGNV
                     CQIGDLLLDKLSNKSNEKAAV"
     gene            complement(3768..4769)
                     /locus_tag="VK031_09245"
     CDS             complement(3768..4769)
                     /locus_tag="VK031_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HLS54146.1"
                     /translation="MKTIIKVLIVSLIFLGLIFSIGNKSYIKDNKDKLGTEINPPKRD
                     EEIIENDEIDWKESKEPENYNTLEEAFNHSKRVNFLILGLEELNSDTIMLASMCQDEE
                     SIDLISLPRDTYIHRKGYDAGEQRKINAVYYNHGIAGVKKTVSYILKGIPIHHYLILD
                     YEGVEAIVDTVGGVEIDVPFHMKYDDPYSNPPLHIDIPAGRQTLNGKMALDFIRYRQG
                     NQGGGYPDGDLGRIRAHQEFLKSFATRAKKNILSLIPSVLKYVETDVSILKALGYGKS
                     MLNLENENINFILLPGSPEFRRVQGKRFSYYIYDKEKIDFMLKEIYRVVEKDKKDSL"
     gene            complement(4844..>5787)
                     /locus_tag="VK031_09250"
     CDS             complement(4844..>5787)
                     /locus_tag="VK031_09250"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004839518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HLS54147.1"
                     /translation="LRDLLDLIEGKDISLNVISKSGTTTEPAIAFRILKDYMEKRYGK
                     KEARSRIFITTDGEKGALKSLAEDEGYESFIIPDDIGGRYSIFTPVGLLPIAVAGLDI
                     ESLIEGARSGQKEYGQVDMDKNISYQYAAIRNILYGKGKNIEILANYKPQLHFLSEWW
                     KQLFGESEGKDGKGIFPAAVSFTTDLHSMGQLIQDGRRNIFETVINVEEAKKDMLLEA
                     DKENLDGLNYLAGKSLDFVNKKAFEGTLKAHVKGGVPNIVINLPRLDEFYFGKLIYFF
                     KKGCALSGYLMGVNPFDQPGVEEYKRNMFQLLGKPGY"
BASE COUNT         1752 a         1177 c          801 g         2057 t
ORIGIN      
        1 cctgctgatg ccctctttac tgcatcaatt aggattaggg tttccattaa gttgtcatta
       61 acaggcgtac aagtgggctg aataatgtat acatccattc ctcttactgt ctcaccgatg
      121 tttacattaa cttctccatc cataaagtaa gtaacctcgc attttgccat ctccacattt
      181 aggcttttag ctattttttc agccaactcc ctatgggcat ttcctgttat tagcctaata
      241 ttgttagtca ccaaattcat tatgtttctc ctcctaggat ttttaattgt tcttctttag
      301 tccccttttc tctacccaac cctctttatt aacctgggta cctctttcta tggctaaggc
      361 tcctttttct acctccttag taattgtaga accagcagca atataagcat agtcatttac
      421 cttcaagggt gccactagat tagaattact tccaacaaat gcattatctc ccacagttac
      481 aacatgctta tttacaccat catagtttgc aaatattacc ccacagccga tatttacatt
      541 cctgccaact atggcatccc caacatatgc caagtgaccc gcctttgtat tatcacctat
      601 agttgcattt tttacctcta caaaattccc tattttaaca tttttaccta gcctgctatc
      661 tggtcttaaa tgagcattag gtccaatatt tgagccctgg cctaccttac ttgcttctat
      721 caggctagat tcaaccacaa ccccatcttc tattttgcta tctactatcc ttgtaccagt
      781 tcttataaca caatctcttc ctatactggt attaccctct aaaacaacac ctggataaat
      841 tgttgtatca gctccaataa taacttttgg ttctatgtag gtagccgatg ggtctattaa
      901 ggttacccct ttctccatat gggccttatt aatcctttct ctcattacct tttcacaaaa
      961 agccaactga tttctagaat tcacaccata aatttctata gaatcttcta ttatatagcc
     1021 cccgattttg taaccttctc tgtttaatat ttcaacgaca tctgttatat ataattcatt
     1081 ttgtgcatta ttgtcatcta gtttttttaa agcacctttt agtagcctac cattgaaaca
     1141 aaatatacca gaattaattt cattaatttc tttctcttcc tcactggcat ccttgtcttc
     1201 tactattttt gatatattcc cttttttgtc cctttttatt ctaccatatc ctgcaggttc
     1261 atccaaaagt gcagtcagta ctgttccatg atattgtcct tctttgtggt aagctacaaa
     1321 tttctttaaa gtatcagctg taatcaatgg cgtatcccca cataagatga ccactgtact
     1381 atcatcttca atcatatcct ccgcctgcat tacggcaaag ccagtaccat agggtacatc
     1441 ttctcctata ggctgatctt taaagattat aggtttatct ttaaagtgtt tctttacttt
     1501 ctctcctcca tgaccagtta tgacaatgtt tttttctatt tttgcaccta tgctagcatc
     1561 cagcacataa tcaagtaaag tcatgccaca tatcctgtgt aagaccttag gcctttttga
     1621 cctcattcta gtgccttcac cggctgcaag tactatagca atatccatta taacacctct
     1681 ttttagatta tccctattac tattttaacc tatttatatt atatgtccac ataaaactat
     1741 tttttaaaga taattagaat ttttagacaa taaaatagag ccatttggcc ctattttatt
     1801 ctgtcgctaa agctttttca tattcagcaa atactgcatc ttgtatcttt tgacgtgtgg
     1861 tggcatttat agggtgagcg atgtctctaa attcaccatc tgccattttc ctactgggca
     1921 tggctataaa caagccattc tgcccatcaa taatcttgat atcatgtaca acaaattcat
     1981 catcaaatgt tactgaaaca atagccttca ttttcccttc ctcagttatt ttccttaccc
     2041 taacatcagt aatattcatt atgtttcacc accttccatt gaagcaacaa aatggtaatt
     2101 atttactata ttctccatta aattgaaaaa tccttctttt ttgtgaaatt tttctataaa
     2161 attattattt ttttaaatat gtaactaaat tgttcttttc aactgcttct ggtatgatat
     2221 aatcaatcag tgacaatttg ttcatattag aatacaataa tattattaga tatcttggta
     2281 acaataaaat atataaagaa ttcctgggag gcaattatat gtttaacttt gacttaaaca
     2341 ataaagaata taatcatatc cattttatag gcatcggtgg catcagcatg agtggattgg
     2401 ctgaaatttt actaaacaaa ggctataagg ttactggtac agacttaaaa gacaacccaa
     2461 ttttaaaaag actagaaaaa ttaggagcaa ccatttatat aggtcatagc aaaaataata
     2521 ttaatggagc agaccttatt atatatactg atgctatttc tactgataat gaagaactgc
     2581 aggcagccag acaatcaggt attcctactg ttgatagggc catgtttctt ggggctttaa
     2641 tgaagaatta tacatattct attgcagtat ctggcaccca tggtaaaacc actaccacca
     2701 gtatgattgc aagtattact aatcatggca ccttagatcc tactatctta ttaggtgggg
     2761 aattggatga tatagggggc aatgtaaaat taggcagtca ggattatatc ctaactgaag
     2821 cttgcgaata caaaggtaac atcttaaagt attttcctac aacggcaatt atattaaata
     2881 ttgatgaaga tcatttagat tattttagag atatagatca tatagtagat actttcgcag
     2941 gttatgctgc caatctagag gaagatagtt acttattaat caatggagat gatcctcatg
     3001 ctccaaaaat aattgaaagt accaaagcac aggtagttaa gtttggtata tctcataagg
     3061 cccattatag ggcggaaaat attagctttt ctaaagaagg ttatccatct tttactttaa
     3121 atattaacaa taaggagttt tggccaatta acttaaaagt tatgggcaag cataatattt
     3181 ataatgcttt agccagtatt gctgcagccc atatacatgg agtttctatg gaggatatta
     3241 cagactacat agagctttac aatggtgtcc atagaagact tgaagtcaag ggcctagtta
     3301 aggatataaa aattatggat gattatgccc atcaccctac agaaatcaag gctaccttag
     3361 atgctttaag agatgttccg aaaaataaat tatattgtat tttccaacca catactttta
     3421 ctagaaccaa ggctttacta gataagttcg cttcttcttt tatgaatgca gaccaaatca
     3481 tcataactga tatttatgcg gctagggaaa atgataatgg agagatccac tcaaaagatc
     3541 tggtccaggc cattaatgac aagggagcag atgccatata tctttcaacc tttgaggaag
     3601 ttaaaaaata tttaatgctt aatatagaag caggagatat tgtcttaacc atgggggcag
     3661 gcaatgtttg tcaaataggt gatttgttgt tagataaatt atctaataag tctaatgaaa
     3721 aagcagctgt ttaattaaat ataggcttaa aagataaaaa ggattcttta taaagaatcc
     3781 tttttatctt tctcaactac cctgtaaatt tcctttaaca tgaaatctat cttttctttg
     3841 tcatatatat aataagaaaa tctcttcccc tgaacccttc taaattctgg acttccaggt
     3901 agcaagataa aatttatatt ttcattttct aaatttaaca tacttttacc atagcctaag
     3961 gcctttaaaa tactaacatc agtttccaca tactttaaga cactgggaat caagcttaga
     4021 atatttttct tggccctagt tgcaaaggac ttaagaaatt cttggtgggc cctaatcctg
     4081 cccaaatcac catcaggata gcctcctcct tgatttccct gcctatatct aataaaatct
     4141 aaggccattt tcccatttaa agtttgtcga ccagccggta tatctatatg caaaggagga
     4201 ttgctatagg ggtcatcata tttcatatga aagggtacat ctatttctac cccacctact
     4261 gtatcaacta tggcttcaac cccttcatag tccagtatta agtaatggtg aataggtata
     4321 ccttttaata tataggatac tgtctttttc acaccagcta taccatggtt ataataaaca
     4381 gcatttatct tcctttgttc tcctgcatca taacccttcc tgtggatgta tgtatctcta
     4441 ggtaaagaaa tcaaatcaat actttcttca tcttgacaca tggaggccag cataatagta
     4501 tcgctattta actcttccaa gcctaagatt aaaaagttta ctcttttact atgattaaag
     4561 gcttcttcta aagtattata gttttccggc tccttgcttt ctttccagtc tatttcatca
     4621 ttttctataa tctcttcatc tcttttagga ggatttattt cagttcctaa cttgtcttta
     4681 ttatctttaa tatatgattt atttccaatt gagaaaataa gccctaaaaa aatgagggac
     4741 acaataagta cctttatgat ggttttcatt aaattcacct ttcttctgct atatccctta
     4801 ttgtatccca attaaataat tatatttaaa ttaatccctg cctttaatag cctggtttgc
     4861 ccaataattg aaacatattt cttttatatt cttctacccc tggttgatca aagggattta
     4921 ctcccattaa atatcctgat agggcacaac ctttcttgaa aaagtaaatc aacttaccaa
     4981 aataaaattc atctaagcga ggtagattta ttactatatt aggcacccct cctttaacat
     5041 gggcctttaa tgtaccttca aaggcttttt tattaacaaa atctaggctt ttacctgcca
     5101 aatagttaag tccatctaaa ttttccttat cagcctctaa aagcatatct ttcttagcct
     5161 cttccacatt tattacagtt tcaaatatat tccttctacc atcttgaatt aactgaccca
     5221 tagagtgtag atctgttgta aaactgacag ctgcaggaaa tatgcccttg ccatcttttc
     5281 cttccgactc tccaaatagc tgtttccacc attcagatag gaaatgtagc tgaggcttat
     5341 aatttgctaa aatttcaata tttttccctt ttccatatag tatattccta atggctgcat
     5401 attgataaga tatgttttta tccatatcca cctgtccata ttctttttgt ccagacctag
     5461 ccccttctat tagactctca atatctagac ctgctactgc aataggtaat agacctacag
     5521 gcgtaaaaat agaatatctt ccacctatat catctggaat tataaagctt tcgtatcctt
     5581 catcttcagc caaagatttt agggctccct tttctccatc tgtagtaata aaaatacggg
     5641 atctagcctc tttttttcca taccttttct ccatatagtc ttttaatatt ctaaaggcaa
     5701 tggctggttc tgtagtggtg ccagacttag atataacatt aaggcttata tcctttcctt
     5761 ctattaaatc taatagatcc ctaagat
//
LOCUS       DATGSI010000598         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_512866, whole genome shotgun sequence.
ACCESSION   DATGSI010000598 DATGSI010000000
VERSION     DATGSI010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512866"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VK031_09255"
     CDS             <1..973
                     /locus_tag="VK031_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLS54148.1"
                     /translation="TTKEMYVQGASSTLPEEVQLKMYKEIIGLENVKFMRPAYAIEYD
                     AIDPTILKRTLEHKEIENLFFAGQINGSSGYEEAAAQGLIAGINAAQNLKGKEPFILD
                     RSEAYIGVLIDDLVTKGADEPYRMMTSRAEYRLTLRQDNADLRLTKKGYEIGLVTEER
                     YQKALEKERAVNGELERLKNVQITPTAENNEKLKELGSTEIRTPVSLYDLIKRPELNY
                     DSLAIFDPDRKHLLKEIRLQVETQIKYEGYIRKQMIQIEQFKKLENKKLAMDFDYSMV
                     KGLSNEAVQKLNEIKPDSVGQASRISGVSPADINVLLIYLEQKRRKE"
     gene            978..1694
                     /gene="rsmG"
                     /locus_tag="VK031_09260"
     CDS             978..1694
                     /gene="rsmG"
                     /locus_tag="VK031_09260"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HLS54149.1"
                     /translation="MLNLDTLVKGVNQLDIELKEEELDKFLKYKELLIEWNEMINITA
                     ITENREVDIKHFLDSLTPLTTKLFEGNKKVIDIGTGGGFPGLPLKITNDNLEVLLLDS
                     LNKRIKFLNEVISQLQLSNIKALHGRAEELSRRTEYREEYDIAISRAVASLDTLSEYC
                     LPFVKPGGYFIAMKGPDMEEELEKSLNSINILGGRLKDKIYITIPESDIVHSLIIIEK
                     IRQTPRKYPRGGGQPRKNPL"
     gene            1794..2594
                     /gene="noc"
                     /locus_tag="VK031_09265"
     CDS             1794..2594
                     /gene="noc"
                     /locus_tag="VK031_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HLS54150.1"
                     /translation="MRLHQEVKYIPITAIRPNPYQPRKNFSKRSLDELSQSIKSYGVI
                     QPISVRQITRDSYELIAGERRLRASQMAELEEIPALVVEYRDRESAMIALIENLQRED
                     LNFIEEAEGYNNLIKDHNFTQQELAEKIGKSQSTIANKLRLLKLPENIQKTLVEADLT
                     ERHGRALLKLPDDALKEQVLEEIINSNLNVKATEELVEDILEELTKEEEPEEEKPRQN
                     IKSLINVRIYLNSLKKAYRAIKEFGVNAEYKEYDKDDHVEVVIKIPKT"
     gene            2693..>3279
                     /locus_tag="VK031_09270"
     CDS             2693..>3279
                     /locus_tag="VK031_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLS54151.1"
                     /translation="MGKIISIFNQKGGVGKSTTSINLSAGLGRLGKNILLVDLDPQGN
                     STSGLGLEKEEIQCQLYDVLAIGRPIEEAIYNTSAKNVQIIPSDNELAGLEVELAREG
                     SWEELLRNRLLEIRDKFDYIFIDCPPSLGILSVLGLIASDSVIIPIQCEYYALEGVSQ
                     LFTTIELVKGSYNPELEIQGIVLSMFDGRTNLSIQV"
BASE COUNT         1292 a          418 c          674 g          895 t
ORIGIN      
        1 tacaactaag gagatgtatg tccaaggagc cagctcaact ctgccagaag aagttcaact
       61 taaaatgtat aaagaaatta taggtcttga aaatgtcaaa tttatgaggc ctgcctatgc
      121 tatagaatat gatgccatag atcctactat tttgaagagg accttagaac ataaggaaat
      181 agaaaatcta ttctttgcag gacaaataaa tggttcatct ggttatgagg aagcagcggc
      241 ccagggattg atagccggta taaatgcggc acaaaacttg aaagggaagg agccctttat
      301 tcttgataga tctgaagctt atattggagt tttgatagat gatctagtaa ccaagggggc
      361 agatgagcct tataggatga tgacatctag ggcagaatat agattaactt taagacagga
      421 taatgcagat ttaagactta caaaaaaggg ttatgaaatt ggcttagtaa cagaagaaag
      481 atatcaaaaa gccttagaaa aggaaagggc tgtaaatggc gaattagaaa gattaaaaaa
      541 tgtacaaata acaccgactg cagagaacaa tgaaaagctg aaagaactgg gaagtacaga
      601 gattagaaca ccggttagtt tatatgattt gattaagagg cctgaattaa attatgattc
      661 tctagctatt tttgatcctg acagaaaaca cctgttaaag gaaatcagat tgcaggtaga
      721 aactcaaata aagtatgaag gatatattag aaaacaaatg attcagattg aacaatttaa
      781 aaagttagaa aacaaaaaat tggccatgga ttttgattac agtatggtta aaggactaag
      841 taacgaagct gttcaaaaat taaatgaaat aaaaccagat tctgtaggtc aagcctcccg
      901 tatatctggc gtatcaccag ctgatataaa tgttttacta atatatctag aacagaaaag
      961 aagaaaggaa taagtaaatg ttaaatttag atacactggt taaaggagta aatcaattag
     1021 atatagagtt aaaagaggaa gaactggata agtttcttaa atacaaggaa ttgctgattg
     1081 aatggaatga aatgattaat ataactgcta taacagaaaa tagggaagta gatataaaac
     1141 attttttaga cagcctaact cctttaacta ctaaattatt tgaaggaaat aaaaaagtaa
     1201 tagatatagg aactggtggt gggtttccag gcttaccatt aaaaataact aatgataatt
     1261 tagaagtact tttattagac agtttaaata aaaggattaa atttctaaat gaggttattt
     1321 cccaactaca gttaagcaat ataaaggccc ttcatggtag ggcagaagaa ttaagcagaa
     1381 ggaccgaata tagggaagaa tacgatattg ccatttccag ggctgttgca tccttggata
     1441 cccttagtga atattgttta ccctttgtta aacctggagg gtattttata gctatgaagg
     1501 gtcctgatat ggaagaagaa ttggaaaaaa gtttaaatag tattaatata cttggtggta
     1561 gactaaagga caagatatat ataactattc cagaaagtga tatagtacat tctttgataa
     1621 taatagagaa aatacgtcaa acaccgagga aataccctag aggaggagga caacctagga
     1681 aaaacccctt ataaaatggg ggaaaatgtt tgaaattact tgggaaataa aaaaggaaat
     1741 agacaagata aatagaatat cttaagtaaa gattacaggg agaggtgtaa tagatgagat
     1801 tacatcagga ggtaaagtat attccgatta cagccataag accaaatcct taccagccaa
     1861 ggaaaaactt tagcaaaaga tcattggacg aattgtcaca gtctataaaa tcctacggag
     1921 ttattcagcc tattagcgtt aggcaaatta ctagggatag ttacgaatta attgctggag
     1981 aaagaaggct aagggcttca caaatggcag aactggaaga aataccggca ttagttgtag
     2041 aatataggga tagagaatcg gcaatgatag ccttaattga aaatttacaa agagaagatt
     2101 taaattttat agaagaggct gaaggctata ataaccttat aaaggatcat aattttaccc
     2161 aacaggaact ggcagaaaaa ataggaaagt ctcaatctac tatagctaat aagctgaggc
     2221 tattaaagct accagaaaat attcaaaaaa ctttggtaga agcagactta acagaaagac
     2281 atggtagagc cttattaaaa ctaccagatg atgcccttaa agaacaagta ttagaagaaa
     2341 taataaacag taatttaaat gtaaaggcca cagaagagtt agtagaagat attttagaag
     2401 aactaactaa agaagaagag ccggaggaag aaaaacctag gcaaaatatt aaatctttaa
     2461 ttaatgttag aatttattta aatagcttga agaaggccta tagagccatt aaagagttcg
     2521 gtgttaatgc agaatacaaa gaatatgata aggacgacca tgtagaagtt gttataaaaa
     2581 ttccaaaaac ataagattta tttaaaaatg ttccacgtgg aacatttttt tcttttgttt
     2641 tgatttaaat atgttattat taatataaga ctataattaa agggtgaaat atatggggaa
     2701 aataatctct atctttaatc aaaaaggtgg agtaggtaaa agcaccacca gcataaactt
     2761 atctgccggt ttagggagat taggtaaaaa tattttatta gttgatttag atccccaagg
     2821 aaattctaca agtggtttag gcctagaaaa ggaagaaatt caatgtcaat tatatgacgt
     2881 tttggcaatt ggcagaccaa tagaagaggc tatatataat acttcagcaa aaaatgttca
     2941 aattataccc tcagacaatg aattggcagg cttagaggta gaactggcga gagaaggtag
     3001 ctgggaagag ttgttgagaa ataggctact ggaaataagg gacaagtttg actatatatt
     3061 tatagattgt ccaccttctt taggaatttt gtcggttcta ggtttaattg cctctgatag
     3121 tgtaattatt cctattcaat gtgaatacta cgccttagaa ggggttagtc aattgtttac
     3181 caccatagaa ttggtaaagg gaagctataa tccagaacta gaaattcaag ggattgtttt
     3241 aagtatgttt gatgggcgga ccaacctgtc tatccaggt
//
LOCUS       DATGSI010000599         1632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_38411, whole genome shotgun sequence.
ACCESSION   DATGSI010000599 DATGSI010000000
VERSION     DATGSI010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38411"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..>1632
                     /locus_tag="VK031_09275"
     CDS             140..>1632
                     /locus_tag="VK031_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HLS54152.1"
                     /translation="MGRKTIFFRCCLLFSIFFTIIILLPLKTEASSGPYKVYDLELNE
                     GKKSVKFVEKARKNKFSDALKEMKKYTNGVVTYEKSPSPLKIVAASRSQAQSYPYRKG
                     KDPAGVTLNIYNNKGLTSAHTYIPAHYMMYVHDFLESNGRIIAQVEIQGGRGYVEVNK
                     VDIIPMIYIENGIEIEIGGNEDYYSKKESPYKVVPKQEEYIVNYNSKYKVNELTVNVY
                     RPSPDVSSFSLTYAIGPDKLKPGTYYSADGINFYKDQDLKKEAYKGFYAYFQWLPLRS
                     MTNHEPKAFDRLLEDYGKRNSVMMGQTKHFIDQGLKYGMNPVFIFQQANLESAYGTSK
                     YARERNNLFGWGAVDSDPDQAYTYDNIGEGIGIHMSSQISGYMNVNDWRHFGPAFGNK
                     SAGITVKYASDPYYGIKVASMYYRMDKLNGFKDYNAYNLSLLADGKSYSVKKNKSTSS
                     EWYKTRSGLKNQLIINLGEENNRIKTPLWMPNYDGVSYPGSFPLNLF"
BASE COUNT          542 a          259 c          335 g          496 t
ORIGIN      
        1 aagaatctaa agcctccttt taaaattaac cgctacttgt attaatattt caggctattt
       61 gttcgatata tatggtatgg ccaagtaatt ttataatcat gccagtataa tttatcttat
      121 gttaggaggt ttgcatttaa tggggcgtaa gacaattttt tttagatgct gtttactatt
      181 ttccatattt tttacaatta ttatcttgct tccccttaaa acagaagcaa gttctgggcc
      241 ctataaagtt tatgacttgg aattaaatga gggtaaaaaa agtgttaagt ttgtagagaa
      301 ggctaggaaa aataaatttt ctgacgcttt aaaggaaatg aagaaatata caaatggagt
      361 agttacatat gagaaaagtc cctccccctt gaagattgtg gcagctagcc gtagccaggc
      421 ccagtcttat ccttatagga aggggaagga tccggctggt gtcaccttaa atatttataa
      481 taataaggga cttacatctg cccacaccta tatacctgcc cattatatga tgtatgttca
      541 tgacttttta gagtccaatg gtaggattat agcccaggtg gagatccagg gcggtagggg
      601 atatgtggag gtaaataaag tagatataat tccaatgatt tatatagaaa atgggattga
      661 aatagaaatt ggcggtaatg aggattacta ttcaaaaaag gaaagccctt ataaggtagt
      721 acctaagcag gaagaatata ttgttaacta taatagtaaa tacaaggtta atgagttgac
      781 tgtcaatgtc tacagacctt caccagatgt aagtagtttt tccctaacct atgccatagg
      841 accagacaag ttaaaacctg gaacctatta ttcagcagat gggattaatt tttataaaga
      901 tcaagacctt aaaaaggagg cctacaaagg cttttacgcc tattttcagt ggttgcctct
      961 aagatctatg acaaaccatg agccaaaggc ctttgataga cttttggagg attatgggaa
     1021 aaggaactct gttatgatgg gtcaaaccaa acattttatt gatcagggtt tgaaatatgg
     1081 aatgaatcca gtatttattt tccagcaggc caatttggaa tcagcctatg gaacatccaa
     1141 atacgctaga gaaagaaata acctctttgg ctggggtgca gtcgactcag acccagatca
     1201 ggcctatacc tatgacaata taggtgaagg aattggaatc catatgagtt ctcagatttc
     1261 aggctatatg aatgtaaatg attggagaca cttcggacct gcctttggta ataaaagtgc
     1321 agggattaca gttaagtatg catctgatcc atattacgga attaaggtgg ccagcatgta
     1381 ttataggatg gacaagctta atggcttcaa agattacaat gcctataatc tttcgctgtt
     1441 ggcagatggt aagtcttatt cagttaagaa aaacaaatcc acaagtagtg aatggtataa
     1501 gaccagatca ggtttaaaaa atcaacttat tattaattta ggcgaggaaa acaataggat
     1561 taaaactccc ttatggatgc ctaattatga tggtgtatcc tatccaggct cctttcccct
     1621 taatttattt ga
//
LOCUS       DATGSI010000600         1145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_370428, whole genome shotgun sequence.
ACCESSION   DATGSI010000600 DATGSI010000000
VERSION     DATGSI010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370428"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..>1145)
                     /locus_tag="VK031_09280"
     CDS             complement(250..>1145)
                     /locus_tag="VK031_09280"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HLS54153.1"
                     /translation="KAIYLSLPIFVGVAINDINAIVDKRLASYLVKGSISALNYANKL
                     KSLILSIFISAITTVVYPMLSREVSDENMDGVKKIINYGVDLIFLITIPATVGIVILA
                     EPIVKVAFQKGEFDSLATMMTAQALLFYSISIVISALRSLVTRVYYSLHDTKTPTINS
                     AITVATNIVFNLILMGPMGHAGLALATSISITPSTIWMYYRLRNKIGNLGLKSHLTNG
                     LKISAISLVMGVISYFSYNGFFQLFGPGLMGTAIALGLAVFLSVIAYGTMCYVFNVNV
                     VRDMVNTGTYNLKKLVSERKK"
BASE COUNT          371 a          220 c          148 g          406 t
ORIGIN      
        1 gagattcata tagttcatcc atcagttttc ctcctaacta ccattattgt acctattcac
       61 caattcttaa tggccacctt tacctttata taaaggtatg gtatataaaa tcaataattt
      121 aattataaca caattaaatt attggcacaa ttattcaata ttcttgataa tttacctgct
      181 aataacgtgg ctcacttcaa tttttctctt cacaaggata atttgtatcc tatttttcct
      241 tatactagtt tatttctttc tttcgctcac caactttttc aagttataag tacctgtatt
      301 aaccatatct cttacaacat tcacattaaa tacataacac atggttccat aggctattac
      361 acttaagaaa actgctaatc caagagctat agctgtaccc attaggcctg gtccgaatag
      421 ttggaaaaat ccattgtaac taaagtagga aataaccccc attaccaagg agattgcact
      481 aattttgagc ccatttgtta agtggctttt taatcctaaa tttcctattt tatttctcaa
      541 tctataatac atccaaatag tggatggagt tatagatata ctagttgcta aagccaagcc
      601 tgcatgtccc ataggaccca ttagaatcaa attaaacact atattagtag ctactgtaat
      661 agcactgtta attgtaggag ttttggtatc atgtagtgaa tagtagactc tagtaacaag
      721 ggacctaagt gcacttatta ctatgcttat ggagtaaaat aatagtgcct gggccgtcat
      781 catagtagcc aaactatcaa attcaccctt ttggaaagct acctttacta taggctctgc
      841 cagtataact atacctactg ttgctggtat ggttattaaa aatattagat ctactccgta
      901 atttatgatt ttctttaccc catccatatt ttcatcacta acttctctag ataacattgg
      961 ataaaccacc gtagttattg ctgaaataaa aatactaagt attaagcttt ttagtttgtt
     1021 tgcataattt aatgcggata tacttccctt aactagataa gatgctaatc ttttatccac
     1081 tatagcattt atatcattta tagcaacacc aacaaatatt ggtagactca aataaattgc
     1141 cttct
//
LOCUS       DATGSI010000601         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_655249, whole genome shotgun sequence.
ACCESSION   DATGSI010000601 DATGSI010000000
VERSION     DATGSI010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655249"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..417
                     /gene="tlp"
                     /locus_tag="VK031_09285"
     CDS             184..417
                     /gene="tlp"
                     /locus_tag="VK031_09285"
                     /inference="COORDINATES: protein motif:HMM:NF039720.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small acid-soluble spore protein Tlp"
                     /protein_id="HLS54154.1"
                     /translation="MKNKPNPDDRRDNVERIQFNIDNTIENYRETKRMIAITDDEKTK
                     KDLIEKNKRRRESLKNMREEIRDEAIAREEGYK"
     gene            complement(493..1746)
                     /locus_tag="VK031_09290"
     CDS             complement(493..1746)
                     /locus_tag="VK031_09290"
                     /inference="COORDINATES: protein motif:HMM:NF022889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HLS54155.1"
                     /translation="MLLKEILRLIIWSAENAFIQVTVFVGAVLMLFGYVNFRLSGRLV
                     EAIGNSKKTQPIVGSLLGLTPGCGGAIFVMPLFPQGVVSFGTIVATLIATMGDSAFVL
                     MSVMPFHYLLVSIFSFIVAIIVGYLVDQTTIGEQLLENINLKQKQRQEEHLSKKEEDH
                     LLQAYESGRTADSLKHIGHREGDAIDLILHHSIKGHQAKDTLGYKITHTAYPIYWLII
                     SVGLIFGILDLFQVDLNNLFIPNLGTILGVIGTFSSVFMMIMSNKFIQDDTHEEAELK
                     AMSLKETIIHNAQETAFVATWVFVAYLVYELFVLGLGGGVYALGETKITSFLSQTGFM
                     AVIIGTLIGIIPGCGPQIIFVSLYTKGLIPFAALLANAVSQDGDALFPLLAIDKRASL
                     WATVFNTMAAFVVGIFAYFVELKFF"
     gene            1953..2645
                     /locus_tag="VK031_09295"
     CDS             1953..2645
                     /locus_tag="VK031_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HLS54156.1"
                     /translation="MFGQDISLPIAFGAGFLSFFSPCILPMIPAYIMYLTGSSLEEAK
                     EKRLYALSRTIGFVIGFTIIFMIMGTSASFLGKIFIRNKSVFSKISGILIIIFGLNMM
                     GIFKIDILNMERRVKGPKAVSSWLSSTVMGMAFAAGWTPCFGPALASILLYAGSVETI
                     SKGVWLLFIYSVGMGIPFILTALFINKFIEIINRAEKLLIYIPKIGGLIMVIFGLLIF
                     FNKVIIISNWFL"
     gene            2659..>2852
                     /locus_tag="VK031_09300"
     CDS             2659..>2852
                     /locus_tag="VK031_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54157.1"
                     /translation="MKNKKIIYSLIAIIIVAGALYLAFNKNDKDTQYGIVDKGVEIAL
                     EEKSPDFILQDLKKNDVRLS"
BASE COUNT         1073 a          433 c          487 g          859 t
ORIGIN      
        1 ggaggaaaag tattttacta agtttgttga actattaagt gaatatatag attgggaaag
       61 gtttgtataa aatttttaaa ataaaaaaat actttttaat ccttaacttt tattatttac
      121 taggataata attgtatcta agaacaaaat aaaaacataa aaataaaaga taaggagatg
      181 aatatgaaaa acaaacctaa tccagatgat agaagagaca atgtagaaag aatacaattt
      241 aatatagata atacaataga aaattacagg gaaactaaga gaatgatcgc aattacagat
      301 gatgaaaaaa caaaaaaaga tttaatagaa aaaaataaaa gacgtagaga gtccttaaaa
      361 aatatgagag aagaaatcag agacgaagca atagctagag aagaaggata caaataaaaa
      421 taagaacctc cataaggctc ctctaactcc atatttggac ttggaggagt ttaaaggagg
      481 ttctttactt tatcagaaaa atttcaattc aacaaaataa gcaaaaatgc ccactacaaa
      541 agctgccatt gtgttaaaaa ctgtagccca caaggaagcc cttttatcta tggccaaaag
      601 aggaaacaag gcatctccat cctgagatac tgcgttagct aaaagagcag caaaaggaat
      661 aagcccttta gtatacaaag atacaaaaat aatttgaggt ccacatcctg gtataattcc
      721 aattaaagta cctattataa cggccataaa acctgtttga cttaaaaaag aagttatttt
      781 tgtctcacct aaagcataaa ctcctccgcc taggcctaaa acaaaaagtt cataaactag
      841 gtaagccaca aaaacccagg tggctacaaa agcagtttct tgagcattat ggataatggt
      901 ttcttttaaa ctcatggcct ttaactccgc ttcctcatgg gtgtcgtctt ggataaattt
      961 attactcatg atcatcataa aaacggatga aaaagtacct ataacaccca gtatggtgcc
     1021 taaatttggg ataaacaaat tatttaaatc aacctggaaa agatctagaa taccaaaaat
     1081 caatcctact gaaataatca accaataaat tggataggca gtatgagtaa ttttatatcc
     1141 taaagtatcc tttgcctggt gtcctttaat actatgatgt aagattaggt ctattgcatc
     1201 tccctcccta tggccaatat gttttaaact atcagctgtt cgaccagatt cataggcctg
     1261 gagtagatgg tcctcttcct ttttagatag atgttcttcc tgcctttgct tctgttttaa
     1321 atttatattt tccaacaact gttccccaat agtagtttgg tcaactaggt aacctacaat
     1381 tatggcaact ataaaggaaa atatacttac caataaataa tgaaaaggca taacagacat
     1441 aagtacaaag gctgagtcgc ccatggtagc aattagtgtg gcaactatgg taccaaaact
     1501 gacaactccc tgtggaaata ggggcataac aaagatggcg ccaccacaac caggtgtaag
     1561 tcctaaaaga gaacctacaa taggctgagt ttttttagaa ttacctattg cttccaccaa
     1621 tcttcctgat agtctgaaat taacatatcc aaataacatt aaaacggcac cgacaaaaac
     1681 tgttacctga ataaaagcat tttcggcact ccaaataatc aatctgagaa tttcttttaa
     1741 taacaaaata tcaactccca actatgtctt attatatacc ctatgataaa atttttaaat
     1801 tgttatatta atttgtagat agatacatta taatataaga ttataatata gggtatgggg
     1861 gtatatataa atttaaccat atgattacct aatatataac aagtatacct gacttatatt
     1921 ataatacaat atttagtaaa aggaggaagt atatgtttgg gcaagatata tctttgccaa
     1981 tagcctttgg ggccggtttt ttatcctttt tttctccctg tatcttacca atgattcctg
     2041 cttatataat gtatttaact ggcagtagtc ttgaagaagc aaaagagaaa aggttatatg
     2101 ccttaagcag gactattggc tttgttatag gttttacaat tatatttatg ataatgggaa
     2161 cttctgctag ttttcttggg aaaatattta taagaaataa aagtgttttc tcaaaaataa
     2221 gtggaattct aataatcata tttggtttaa atatgatggg catatttaaa atagatattt
     2281 taaatatgga aaggagagta aaggggccta aagctgtaag cagctggtta agttcaactg
     2341 taatgggaat ggcctttgca gcagggtgga ccccatgttt tgggccagcc ctagcatcta
     2401 ttttacttta tgcaggcagt gtggaaacaa ttagtaaggg agtatggcta ttatttattt
     2461 attctgtagg catgggcata ccctttatac ttacagccct gtttataaat aaatttattg
     2521 agataataaa tagggcagaa aaattactaa tatatattcc aaagattggt ggccttatta
     2581 tggttatatt tggtttatta atattcttta ataaagtaat aattataagc aattggttct
     2641 tatagaaagg atggagagat gaaaaacaag aaaataattt actcactgat agccataatc
     2701 atagtggcag gagcattata tttggctttt aacaaaaatg acaaggacac ccagtatggc
     2761 attgtagata aaggtgtaga aatagcctta gaggaaaagt cgcctgattt tattctacag
     2821 gacctaaaga aaaatgatgt aaggttaagc ga
//
LOCUS       DATGSI010000602         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_272657, whole genome shotgun sequence.
ACCESSION   DATGSI010000602 DATGSI010000000
VERSION     DATGSI010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272657"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VK031_09305"
     CDS             <1..621
                     /locus_tag="VK031_09305"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HLS54158.1"
                     /translation="DESLVEFVQDFSKRTGLFTIDLLSPLMNTVSQVLNTEPKREPGI
                     IRRLDEAYFDRVAAIEFAVKYDDGKDPRGLLDADLVLIGVSRTSKTPLSMYLANKNIK
                     VANVPLVMEVKPPKELFEISARKIIGLTNSPENLNEIRLQRLKSLGLNTNSSYASLDR
                     ILKELDYADKVMKKIGCPIIDVSDKAIEETSEIILGLLRKNNINSY"
     gene            647..3274
                     /gene="ppdK"
                     /locus_tag="VK031_09310"
     CDS             647..3274
                     /gene="ppdK"
                     /locus_tag="VK031_09310"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HLS54159.1"
                     /translation="MAKKYVYAFSQGHKDMKNIMGGKGANLSEMTRIGLPVPAGFTIS
                     TEACIRYFKENKSLWPELLDEISQQMENLERLVGKKFSDKNNPLLVSVRSGAVVSMPG
                     MMDTILNLGLNDESVEGLARSTGSRKFAYDSYRRFIQMFADVVMGVPKVRFDSSLNKL
                     KKDKGAVEDEELTGKDFKYLVEEYRKVYLEETKKEFPQDPKEQLFLAIQAVFDSWENP
                     RAKVYRQLHNIEDDLGTAVNVQSMVFGNMGERSGTGVAFTRDPATGENKLFGEYLINA
                     QGEDVVAGIRTPMDISQLKETMPQIYDEFLQISKKLEKHYKDMQDIEFTIENEKLYIL
                     QTRSGKRTTQAAIKIAIDMVKEGLISKEEALLRIEPNDLNQLLHWNFKEEQLKEAQII
                     ASGLAASPGAASGKIYFHAEDVVNAKKRGEEAILVRQETSTEDIEGMVSAEGILTSRG
                     GMTSHAAVVARGMGKCCVAGCNDISVDEGTKTVKSGGLILKEGDYISLDGSTGKVYLG
                     QIDKVQPSLDGSFKDFLELVDKVRDLKVRINADNPKDVRQGLEFGAEGIGLCRTEHMF
                     FDESRILSVRKMILANNLKERQAALAELLPVQRKDFLSLYRAIGERPVTIRLLDPPLH
                     EFLPNRAEEIRELAQAMEVDFERLKVRIEELKEFNPMLGHRGCRLAITYPEIYRMQVR
                     AIIEAALDMKKKGYAGIIPEIMIPLVGHVNELKYVKEEIIDEIEKVFKERKMQVNYSI
                     GTMLEVPRAAITADEIAGEVDFFSFGTNDLSQMGFGFSRDDAGKFLEEYLEKGIFKKD
                     PFQSIDRKGIGRLMEIAIEYGKEGNPNLTLGICGEHGGEPSSIEFCYLIGLDYISCSP
                     YRVPIAKLAAAQAKIRNTR"
     gene            3469..>4173
                     /gene="yaaA"
                     /locus_tag="VK031_09315"
     CDS             3469..>4173
                     /gene="yaaA"
                     /locus_tag="VK031_09315"
                     /inference="COORDINATES: protein motif:HMM:NF002543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HLS54160.1"
                     /translation="MRIIISPAKAMKVDNDVFQPKRLPVFIEKTEEILSYLKGLDYEE
                     LKDLWKTSHKLTQLNFQRVKRMDLYKNLTPAILSFDGLQYKYMGPTVFSYDELDYIDE
                     HLRILSGFYGILTPFDGVVAYRLEMQAKPKNWEYENMYEFWGDKIAKELFSQSNCIIN
                     LASMEYSRVVKKYLKDNVRFINCIFGELIEGRIIQKGTLAKMARGELVQFMAREKIKD
                     PEDIKGYKGLGYVFKSS"
BASE COUNT         1496 a          554 c          988 g         1135 t
ORIGIN      
        1 gatgaaagtt tggtggagtt tgtacaggac ttttctaaaa ggacaggact ttttactata
       61 gatctattaa gccctttaat gaacactgta agccaggtat taaatactga acctaaaagg
      121 gaaccaggga ttataaggag gctggatgag gcctattttg atagggtggc tgccattgaa
      181 tttgctgtaa aatatgatga cggaaaggat cccagaggcc tattggatgc agatttggtg
      241 ttaataggag tgtctagaac ttccaagaca cctttaagta tgtatttagc caataaaaat
      301 attaaggttg ccaatgttcc cctggtaatg gaggtgaaac ctcctaagga attatttgaa
      361 atatctgcaa gaaagataat tggacttaca aattcacctg aaaatttaaa tgagattagg
      421 ttgcagagat taaagtcttt aggattaaat actaactcaa gctatgccag tttagataga
      481 atactaaagg aactagatta tgcagataaa gttatgaaaa aaattggatg tccaattata
      541 gatgtatcgg ataaagccat agaagaaaca tcagaaatca tattaggatt acttaggaaa
      601 aataatataa attcctatta aggagaatgt ggagggggaa ttttcagtgg ccaaaaagta
      661 tgtatatgcc ttcagccaag gacataaaga tatgaaaaac ataatgggtg gcaagggggc
      721 caatctatct gaaatgacca ggataggttt acctgtgcca gcaggtttta ccataagcac
      781 ggaggcttgc attagatatt ttaaggaaaa taaaagccta tggccagaac tgctagatga
      841 gatttctcag caaatggaga atttagagag gcttgtagga aagaagtttt cagataaaaa
      901 caatccccta ttggtatcag tcagatcggg ggctgtggtg tctatgccag gaatgatgga
      961 tacaatactt aacctagggt taaatgatga gtcagtggag ggactggcca ggtccaccgg
     1021 tagtagaaaa tttgcatacg acagttatag aagatttatt caaatgtttg cagatgtggt
     1081 tatgggggtt cctaaggtaa ggtttgattc tagcttaaat aagcttaaaa aagacaaagg
     1141 ggcagtagaa gatgaggagc tgacaggcaa ggattttaag tatttagtgg aagaatacag
     1201 aaaggtctac ttagaggaaa caaaaaaaga atttcctcaa gatccgaaag aacagctatt
     1261 tttagctatc caggcagtat ttgattcttg ggaaaatcca agggccaagg tatatagaca
     1321 attacataat attgaagatg atttagggac tgcagtcaat gtccaaagta tggtgtttgg
     1381 taatatggga gaaaggtctg gaacaggggt agcctttacc agggacccag caacaggtga
     1441 aaacaaatta tttggagaat atttaatcaa tgcccaagga gaggatgtgg tggccggtat
     1501 taggacacca atggatatat cccaactaaa ggagactatg cctcagattt atgatgagtt
     1561 tttacaaatc agcaaaaaat tggaaaaaca ttataaagat atgcaagata tagaatttac
     1621 cattgaaaat gaaaagctat atattctgca aactagaagt ggaaaaagga caacacaggc
     1681 agccattaaa attgccatag acatggtaaa ggaaggctta ataagtaagg aagaggccct
     1741 gttaaggata gaacccaatg acttaaacca gctattacat tggaacttta aagaagaaca
     1801 gttaaaggag gcccaaatta tagcaagtgg gctggcagca tcaccagggg cagcctcagg
     1861 aaaaatatat tttcatgctg aagatgtggt aaatgcaaag aaacggggag aggaagccat
     1921 attggttagg caagagacat ccactgagga tattgaaggg atggtatctg cagaaggcat
     1981 actaacttca agaggaggaa tgacctccca tgctgcagtg gtagctaggg gaatgggcaa
     2041 atgttgtgtg gcaggttgta atgatataag tgtagatgaa ggtacaaaaa cagtaaaaag
     2101 tggtggcctt atcttaaagg agggggatta tatttcccta gatggaagta caggtaaggt
     2161 ttacttaggc caaatagata aggtacagcc aagccttgat ggaagcttta aagacttttt
     2221 agagctggta gataaggttc gagatttaaa ggtaagaata aatgctgata atccaaagga
     2281 tgtaaggcaa ggtctagaat ttggtgctga aggaattggt ctttgcagaa ctgagcacat
     2341 gttttttgac gaatctagaa ttctatctgt tagaaaaatg attctagcca ataacctaaa
     2401 agaaagacaa gcagctttag cggaactttt gcctgtacaa aggaaggatt ttttaagcct
     2461 ctatagggct ataggtgaaa ggcctgttac cattaggctt ttagatccac ctctacatga
     2521 atttttacct aatagggctg aggaaataag ggaattggcc caggccatgg aggtagattt
     2581 tgaaagacta aaggttagga ttgaagagtt aaaagaattt aatcctatgt taggtcatag
     2641 aggttgtaga ttggctataa cctatccaga aatatataga atgcaggtaa gggccataat
     2701 tgaggcagcc ttagacatga aaaagaaggg ctatgcaggt attattccag aaataatgat
     2761 acctttggta ggacatgtaa atgagctaaa gtatgtaaaa gaagagataa tagatgagat
     2821 agaaaaagtt tttaaagaaa gaaaaatgca ggtaaactat agcataggaa ccatgttaga
     2881 ggtgcctaga gcagctatta ctgccgatga aatagctggt gaggtagatt tctttagctt
     2941 tggaaccaat gatttaagcc agatgggatt tggtttttcc agggatgatg caggtaaatt
     3001 tttagaagaa tacttggaaa aaggtatttt taaaaaggat cccttccaat ctatagatag
     3061 aaaaggaata ggaaggctga tggaaatagc cattgagtat ggaaaagagg gtaatccaaa
     3121 tctcacacta ggcatatgtg gtgaacatgg aggagaacct agttccatag aattttgcta
     3181 tttaatagga ctagactata taagttgttc cccatatagg gtccctatag caaaactggc
     3241 agcagcccaa gctaagatta ggaatacccg ttaaagatca agaaaaagtt tttataaaaa
     3301 aggtacagca gttagatggc tgtacctttt ttagtgggag tataattttt gaaatgatat
     3361 ttttactagc ttttcaactt gtaaaagaaa aaaagtagga ctaaaatata gatgttgatg
     3421 ggtatatgta agatatattg tttcgatata aggagttgaa tataaattat gcgcattatt
     3481 atttcaccgg ccaaggctat gaaggtagat aatgatgttt tccaaccaaa gaggctacca
     3541 gtctttattg aaaaaacaga ggagatttta tcctatttaa aagggctgga ctatgaagag
     3601 ttgaaagacc tgtggaagac tagccataaa ttaacccagt taaactttca aagggtaaaa
     3661 aggatggatc tatataaaaa tttaactcct gccatcctat catttgacgg attacaatat
     3721 aaatatatgg ggcctactgt attttcctat gatgaattag attatattga tgagcattta
     3781 aggattctgt caggctttta tgggatactt actccctttg atggggttgt ggcctatagg
     3841 ctagaaatgc aggctaagcc taaaaattgg gaatatgaaa acatgtatga gttttgggga
     3901 gataagatag ccaaagagct tttttcccag tctaactgta ttataaattt agcttctatg
     3961 gaatatagta gagttgtaaa aaaatactta aaggataatg taaggtttat aaactgtata
     4021 tttggagagt taatagaagg aaggattata caaaaaggca ccttagccaa gatggccaga
     4081 ggagaattgg tgcaatttat ggcaagggag aagattaagg atccagaaga cattaagggg
     4141 tataaaggac taggttatgt ttttaaaagt agc
//
LOCUS       DATGSI010000603         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_655295, whole genome shotgun sequence.
ACCESSION   DATGSI010000603 DATGSI010000000
VERSION     DATGSI010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655295"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1023)
                     /gene="uxaC"
                     /locus_tag="VK031_09320"
     CDS             complement(<1..>1023)
                     /gene="uxaC"
                     /locus_tag="VK031_09320"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HLS54161.1"
                     /translation="EKLYHEYAKDMPIFDYHCHLSPKEIAEDKSYRNITEIWLGGDHY
                     KWRAMRSNGIDEKYITGDASDYDKFKAWARTLPYCLGNPLYHWSHLELKRYFNIDTII
                     NEDTADEIWEKTNEMLATDEFTARELLKKMNVKVVCTTDDAIDSLEYHRQIREDKDFD
                     IKVLPTLRPDKGINIQNDEFVDWVNKLSEVSGIEINNYDDFIKALENRVEFFHEEGCR
                     LSDHGIDKMFYEEATDQEVDEIFKKAMSKEKLSQVEMDKYKTRTMIHLARMFNRLGWT
                     MQLHMGAIRNNNSRMFEKLGPDTGFDSIGDGLIAEDLSNLLDAMDKTDQLPKTILYCL
                     NASDNDV"
BASE COUNT          273 a          214 c          147 g          389 t
ORIGIN      
        1 aacatcattg tcactggcat ttaggcaata caggatggtt tttggcaatt ggtctgtttt
       61 atccatagca tctagcaggt tagatagatc ttcagctatt aggccatctc ctatggaatc
      121 aaaacctgta tctggtccta acttttcaaa cattctgctg ttgttatttc taatggcacc
      181 catgtgtaat tgcatggtcc agcctaatct attaaacatt ctagctaggt ggatcatagt
      241 tcttgtctta tatttatcca tctccacctg gcttagcttt tctttggaca tggctttttt
      301 aaatatttca tctacctctt ggtcagttgc ctcttcataa aacattttgt caattccatg
      361 atctgacaat ctacaacctt cctcatggaa gaattctacc ctgttttcaa gggccttgat
      421 gaaatcatca tagttgttaa tttctattcc actaacttct gatagcttat ttacccagtc
      481 tacaaactca tcattttgta tattaattcc cttatctggc ctaagagttg gaagtacttt
      541 tatatcaaag tccttatctt ctctgatttg tctatgatat tctaggctat ctatagcatc
      601 atcagtagta caaacaacct ttacattcat ttttttaagt aattctctag cagtaaattc
      661 atctgtagcc agcatttcat tagttttttc ccaaatttca tctgctgtat cttcatttat
      721 tatggtatct atgttgaagt acctttttaa ctctaaatga gaccagtggt ataaggggtt
      781 acctaaacag taaggtaagg ttctagccca ggccttgaac ttatcataat cagaggcatc
      841 tcctgttata tatttttcat caataccatt gctcctcatg gccctccatt tatagtggtc
      901 tccacctaac caaatttcag ttatattcct ataacttttg tcctcagcaa tctccttagg
      961 acttaagtgg cagtgataat caaaaatagg catatctttt gcatattcat gatataattt
     1021 ttc
//
LOCUS       DATGSI010000604         1292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_230789, whole genome shotgun sequence.
ACCESSION   DATGSI010000604 DATGSI010000000
VERSION     DATGSI010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230789"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            677..>1292
                     /locus_tag="VK031_09325"
     CDS             677..>1292
                     /locus_tag="VK031_09325"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HLS54162.1"
                     /translation="MSIVAKTYRGDIVDLTHIGHIAVVDHKGQILHYFGDPKRVTFAR
                     SSAKPMQAIPVVESGAVEKYGIDKREIALFTASHSGEDFHVKAAESILDKAGLSKNHL
                     QCGIHYPLNAKVRDKMKREGLEAETIHNNCSGKHAGMLVTTKYYGDDLESYYKKDHPT
                     QKRVLKTIADICQYEEEKIILGVDGCGVPVHAMPLYKFAQGYARL"
BASE COUNT          469 a          203 c          265 g          355 t
ORIGIN      
        1 taattcattt atgtacgagc cgataattga tatgtccgac catcatttaa aaaaggttct
       61 caaaggtgct atgcaattgt ggcatgaaga aaatgaatat gagaacttag cagtagaaaa
      121 gaactgatac agaagtaaag attaggcgac aagcgcctaa tctttttacg ataattcaga
      181 acaatattct tgttatcaaa ttcaaataac tcttacctaa gatatccaca tgatggtatt
      241 agcaataagc ccaacaattt aacttaagct ggtataaaat tgttgttggg gaggaaataa
      301 aaaacaaggt aaaaggaccc aattgaactg tatattagtt gaggaactat taaagattat
      361 aagagatatg cccttatgga tatagcaact ggagaaataa aatgagtgaa aaaagcgcta
      421 agaaaggtcc taataggaaa actgatagtt acaaaaaaca tataagtttt cccatggatt
      481 ttacctccta tttttccata tatagtataa tagtttcatg tgtattagtt ttaggttgta
      541 tttttaaatg aaggatatgg aaaatgatat ctgtataaag atttaacttt actcaatgtg
      601 aaaaaggtag gtgatttctt ggtacctagg ccaggatctt aactatctac aaggataaat
      661 aataggaggg gtatacatgt caattgtagc taaaacctat aggggagata tagtagacct
      721 aacccatata ggccatattg cagtagtaga tcacaaggga caaatcttac actactttgg
      781 agatcccaaa agagttacct ttgcccgttc ttcagccaag ccaatgcagg ccatacctgt
      841 agtggaaagt ggggcagttg aaaagtatgg aatagataaa agggagatag ccttgtttac
      901 cgcatcccac agcggggaag attttcatgt aaaggcagcc gaaagcattt tagataaggc
      961 tggattaagt aaaaatcacc tccaatgcgg aatccactat cctttaaatg ccaaggttcg
     1021 ggacaaaatg aaaagggaag ggcttgaagc ggaaacaatt cacaataatt gctctggcaa
     1081 acatgccggt atgcttgtaa ccaccaagta ttatggtgat gatttagagt cctattataa
     1141 aaaggatcat ccaacccaaa aaagggtttt aaaaacaata gcagacatat gccagtatga
     1201 agaagagaaa attatcctag gtgtagatgg ctgtggagtt ccagtccatg ccatgccttt
     1261 atataagttt gcccagggct atgccagact tt
//
LOCUS       DATGSI010000605         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_230845, whole genome shotgun sequence.
ACCESSION   DATGSI010000605 DATGSI010000000
VERSION     DATGSI010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230845"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VK031_09330"
     CDS             complement(<1..293)
                     /locus_tag="VK031_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HLS54163.1"
                     /translation="MKLGFVGLGIMGKPMVRNLLKGGYEVVVYDIIQENIDEVVKDGA
                     KAATSSKAVAEETEIIITMLPNSPHVKTVVMGENGILDGAKEGTIIIDMSSIAP"
     gene            complement(344..>1527)
                     /locus_tag="VK031_09335"
     CDS             complement(344..>1527)
                     /locus_tag="VK031_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HLS54164.1"
                     /translation="AGKAIGVGIVFSAIGTILSIAALIFVAPQLAKLTIKFGPWEYFS
                     VTLFSLTLIASLAGNSISKGLIAGVLGMMFATVGLAPVDSVQRFTFNSIQLSSGFDVL
                     TVLVGLYAISEIIKTAGTLGSGQEMEIQSDFNMKGFGFSFSEFKSQIGNAIRSTLIGI
                     GIGILPGIGGGTSNILSYSVAKNQSKHPEKFGTGIIDGIVASESANNATIGGAMIPLL
                     TLGIPGDGVTAMLLGGFVLHGIQPGPLIFTNNADVVYGVFAAMIIATIAMLVFEFVGI
                     RFFVKILKVPKNLLLPMIIILCSVGAFALNNRTFDIWAVILFGIVGYFMEKFEYPLPP
                     FILGFVLQETFEVNLRRGLQYSQGNIFAVFSQPIAVVFIVGTLVSLFFSLRKQFKVER
                     A"
BASE COUNT          482 a          335 c          218 g          492 t
ORIGIN      
        1 ggggctattg aactcatatc tataataatg gttccctctt ttgcaccgtc taatattcca
       61 ttttcaccca tcaccacagt tttaacatgt ggagaattag gtagcatagt tataattatt
      121 tccgtttctt cagccacagc cttagaagat gtggcagctt ttgcaccatc ctttactacc
      181 tcatctatat tttcctggat tatatcgtat acaactactt catatccacc ctttaataaa
      241 ttccttacca tgggcttacc cattatccct aatcctacaa aacctaactt cattgataca
      301 tctctccttt aatttattat aatcttttat tatatattgc tacttatgcc ctctctactt
      361 taaactgctt tcttaatgag aaaaataatg atactaatgt tcctacaata aataccactg
      421 ctataggttg gctgaaaact gcaaagatat taccttggga atactgcaat ccccttctta
      481 aattaacttc gaaggtttcc tgtaatacaa agcccaatat aaaaggcgga agaggatatt
      541 caaatttctc cataaagtat cctactattc caaaaagtat cacagcccat atatcaaatg
      601 tcctgttatt taaggcaaag gcacctactg aacaaagtat tattatcata ggaagcaata
      661 ggttttttgg tacctttagt atctttacga aaaaccttat tcctacaaac tcaaatacca
      721 gcatagcaat tgttgctata atcatggcag caaatactcc atagactaca tctgcattat
      781 tggtaaaaat taatggtcct ggctggatcc catgtaagac aaaaccacct aataacatgg
      841 cagttacacc atctcctggt atacctaaag ttaatagggg gatcatggcc ccaccgatgg
      901 tagcgttgtt tgcagactca gatgccacta ttccatcaat tattcctgta ccaaatttct
      961 ctggatgttt agattggttt ttggcaactg aataggataa gatattactg gttcctccac
     1021 caattcctgg taaaatacct atgcctatac ctattagggt tgatcttata gcattgccta
     1081 tctgagattt aaattcggag aaactaaatc cgaaaccttt catgttaaaa tcagattgta
     1141 tttccatttc ttgccctgaa cctaaggttc ccgccgtctt tataatctcc gatatggcat
     1201 ataagcctac taatactgtt aaaacatcaa agcctgagct taattggata ctattgaatg
     1261 taaatctctg taccgaatca actggcgcca agcctactgt tgcaaacatc atacctaaga
     1321 caccagctat tagcccctta cttatagaat tacctgccaa acttgctatt aatgttagtg
     1381 aaaataaggt aacactaaaa tactcccaag ggccaaattt aattgttagt tttgctagtt
     1441 ggggagcaac aaaaatcaaa gcagctatac ttaatatagt tccaatggca gaaaatacta
     1501 ttccaacacc tattgctttt cctgcct
//
LOCUS       DATGSI010000606         1157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_38779, whole genome shotgun sequence.
ACCESSION   DATGSI010000606 DATGSI010000000
VERSION     DATGSI010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38779"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VK031_09340"
     CDS             <1..525
                     /locus_tag="VK031_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLS54165.1"
                     /translation="KSLAYEIWEQLGYICPDNIICPVGNGSIALGLVQGFKELLNNSE
                     VDKLPRIFGVEPANSNAIYRKFNGLKEDFEAEPTIAEGIALRYSSKAQEVVKAISESK
                     GEMEAVTEGEIIKALEKISKKGFFIEPTSAAALAGLTRLIDKGEIAQDEKTVVIISGN
                     GLKASDKILQYLVK"
     gene            573..>1157
                     /locus_tag="VK031_09345"
     CDS             573..>1157
                     /locus_tag="VK031_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HLS54166.1"
                     /translation="MKLGFIINPIAGMGGPVGLKGTDGTEILEKALKLGAVARSPKKA
                     KAALEKLIPLKDDLRLITYPGKMGEKVAIELGFKPVVIGELDERTSGRHTEEAAKAML
                     DLGVDLIIFAGGDGTARNIYNAVGSKLPVIGIPTGVKIHSGVYASHPRAAGLIAYKFL
                     TSQDRETMEAEVMDIDEEAFRRGLITAKLYGYVLT"
BASE COUNT          399 a          167 c          297 g          294 t
ORIGIN      
        1 aaatctttag cctatgaaat ctgggagcaa ttaggctata tatgtcctga taatattata
       61 tgtcccgttg gcaatggaag tattgcctta gggctggtgc aagggtttaa agaactatta
      121 aataattcag aggtggataa attaccaagg atttttgggg tggagcctgc aaatagcaat
      181 gccatatata gaaagtttaa tggattaaaa gaagattttg aagcagaacc taccatagca
      241 gaagggattg ccctaagata ttccagcaag gcccaggaag tggtcaaggc cattagtgag
      301 tcaaaaggag aaatggaagc agtaactgaa ggggaaataa ttaaggcttt agagaaaatt
      361 agcaagaaag ggttttttat agaacctact tccgcagctg ccttagccgg tttaactaga
      421 ttaatagata aaggggaaat agctcaggat gaaaaaacag ttgtaattat atcaggaaat
      481 ggcttaaaag ccagcgataa gattcttcaa taccttgtga aataaaatcc taagggtaag
      541 ggtttataag gaacagttag gagaggaaat agatgaagct tggttttatt attaatccta
      601 ttgcaggtat gggtgggcca gttggcttga agggtactga tgggactgag attcttgaaa
      661 aggctttaaa actgggagca gtagccagat cacctaagaa ggcaaaggct gccttagaaa
      721 aattaatccc tttgaaagat gacttacggt taataaccta tcctgggaaa atgggggaga
      781 aggtggccat tgaactggga tttaagccgg tggtaatagg agagttagat gaaaggacaa
      841 gtggcagaca cacagaagaa gctgccaaag ccatgcttga tttaggagtt gacttaataa
      901 tctttgctgg aggagatgga actgccagaa atatttataa tgcagtagga agtaagctac
      961 cagtgatcgg aatacctaca ggagtcaaaa tccattctgg agtatatgcc agccacccta
     1021 gggcagctgg gttaatagca tataagtttc taactagtca agatagagaa actatggaag
     1081 cagaggttat ggatattgat gaggaggcct tcagaagggg gctaattact gccaaactat
     1141 acggatatgt tttaacc
//
LOCUS       DATGSI010000607         1258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_703496, whole genome shotgun sequence.
ACCESSION   DATGSI010000607 DATGSI010000000
VERSION     DATGSI010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703496"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..>1258
                     /locus_tag="VK031_09350"
     CDS             128..>1258
                     /locus_tag="VK031_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54167.1"
                     /translation="METEKFSVSRTGSVTHAPTSDSGSYWNTFTFFGGQRGNQLTINN
                     KEGHLWLKHWNSSTASGNWRRVAEIPDLFSGDYNDLSNKLSAGNNISISTTNEISAVD
                     TKYSAGARMSLSGTTFSANKQTWGDIQNKPNDLAKTGDIKDGTLSISTGSLLTGSGSF
                     SANQGSNESIDIDLTQATKDKINNGTQTLNFNSNNGNLSISGGNIVGLDSRYPIKGSL
                     PSSIHSTDVRKMSGSDGTYSYMPAPNDPVLDESLVLLFSKDVPSSVSGSLASILTAKA
                     WREENLSYTAWQLAGNASTLSSNSDKFAIRTGNGATWNDWKIIATEEWVNAQSYLKSV
                     NLSSSLSGNNLTVSNDAGGSTTVDIGASFLSIDKDNPNPIIDN"
BASE COUNT          418 a          196 c          263 g          381 t
ORIGIN      
        1 actctctatc tcctctagta caggatcagg agatgatata gaccttgagg gtatatttga
       61 tggtagttac ttagatagag cagatgatgg ttttaatgat tttgattcct tgatacctag
      121 ttcaggagtg gaaacagaaa aattcagtgt ttcaagaaca ggatctgtaa ctcatgcacc
      181 cacatcagat tctggttctt attggaatac ttttactttt tttggtggtc aaagagggaa
      241 tcagctgact attaataata aagaggggca tttgtggcta aagcattgga actcttctac
      301 agcttcagga aattggagaa gggtagcaga aatacctgat ttattttcag gggattacaa
      361 tgatcttagc aataagcttt cagcagggaa taatattagt atttcaacta ccaatgagat
      421 ttcagctgtt gacacaaaat atagtgcagg tgcgagaatg tcattatccg gcactacttt
      481 ctctgctaat aaacaaacat ggggggatat acaaaataaa cctaatgatt tagccaaaac
      541 gggggatata aaagatggta ctctatctat ttctacaggg agcttattaa cgggttcagg
      601 ttctttctcg gcaaatcaag gtagcaatga gagcatagat atagacttaa cacaggctac
      661 aaaagataag attaataatg gaactcaaac attaaatttt aatagtaata atggtaattt
      721 atctataagt ggagggaata tagttgggtt ggatagtaga tacccgatta aagggtcttt
      781 accttcttcc atacattcca ctgatgtaag aaaaatgagt ggcagtgatg gcacttattc
      841 atatatgcct gctcctaatg atcctgtatt agatgaatca ttagtattat tgttttcaaa
      901 agatgtacct agttctgtaa gtggtagttt ggcatctatt cttactgcaa aagcttggag
      961 agaagagaat ttaagttata ctgcttggca actagccggg aatgcaagca ctctgtcttc
     1021 aaattctgac aaattcgcca ttagaacagg aaatggtgcg acatggaatg actggaagat
     1081 aatagcaaca gaagaatggg ttaatgctca aagttattta aaatccgtaa atctttcttc
     1141 ttccctttcc gggaacaatc taaccgtaag taatgatgca ggaggaagca ctacggtaga
     1201 tataggagct tcttttctta gtatagataa ggataaccct aatccaataa tagataac
//
LOCUS       DATGSI010000608         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_370876, whole genome shotgun sequence.
ACCESSION   DATGSI010000608 DATGSI010000000
VERSION     DATGSI010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370876"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VK031_09355"
     CDS             <1..460
                     /locus_tag="VK031_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HLS54168.1"
                     /translation="IKSKVTEILQEVNSKPVYTIEEFLYGKKIEPKLINIIGGPAKVL
                     QPILIDKFNLPCYSPKNYQVANAVGAALAKPTAEINMVANTAQGLLSVPEMGIHERIG
                     RSFTLNEAKKRALDLVEKHALSLGALQDEIEAEIIEESSFNMVQGFYTSG"
     gene            522..1829
                     /locus_tag="VK031_09360"
     CDS             522..1829
                     /locus_tag="VK031_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HLS54169.1"
                     /translation="MIKAKNKLGLVFFPAFDWAISPTHPEREERLLYTQDQLFEEGIE
                     DIDGINFYNPTIAEERDISRVHFAVPDVKSRVTQSHLISVGGAIKAFQKVMEGEVDKS
                     FALVRPPGHHAHRVVYGDRGFCIVNVEAVMLEKIRQEYKDLRVAIVDTDCHHGDGTQD
                     IYWNDKDTLFISFHQDGRTLYPGTGHIDEFGGPKAYGYNVNIPLPPGTGEEGFLYVLD
                     HVVLPILDEYQPDIIINSAGQDNHYTDPITNMNFTAQGYAKLNHKLNPDIAVLEGGYS
                     IEGALPYINLGIILAMAGIDYSNVKEPDYDPDKLRQTDDITEYIKQISEHVYKQWRNK
                     EELGHKLFKGKDWVKSNRRIYYDTDNIMEKQTQSFKVCNKCSGLNTIESRNNRGHRIF
                     AITIPRKACSACIDQAYSLYKNSNSTYTNLYLQDRVHDKYYAK"
     gene            complement(1925..>2243)
                     /locus_tag="VK031_09365"
     CDS             complement(1925..>2243)
                     /locus_tag="VK031_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HLS54170.1"
                     /translation="EGFNVVMEDNLFVILDTSLTPELINEGYAREFISKVQQMRKNNG
                     YEVLDKINIYYSGSDEIKEAIKEHEDFIKTETLAKSIIRVDEELEKQDLNGQMTGIKL
                     EKI"
BASE COUNT          717 a          391 c          446 g          689 t
ORIGIN      
        1 gattaagtct aaggtgacag aaattttaca agaggttaat agcaaacctg tttataccat
       61 agaggaattt ttatatggga aaaaaataga gcctaaatta atcaacatta tcggagggcc
      121 tgctaaggtt ttacagccca tattgattga taagtttaat ctgccttgtt attcaccaaa
      181 gaattatcag gtggccaatg cagttggagc cgccttagct aaacctacag ctgaaataaa
      241 tatggtagcc aacacagccc aaggtcttct ttcagttcct gagatgggta tccatgaaag
      301 gataggtagg agctttaccc ttaatgaggc caagaaaagg gccctagatc tggttgaaaa
      361 acatgcctta tctttaggag ccctacagga tgagatagag gctgaaatta tagaggaaag
      421 tagttttaat atggtgcaag gtttttatac cagtggctaa aatatacgga ttcgtgccca
      481 ggtaaaacct ggactaattg aggaatttag aggtgaatct aatgattaaa gcaaagaata
      541 aattaggttt agtgtttttt cctgccttcg attgggccat aagtcccacc catcctgaaa
      601 gggaggaaag gcttttatat acacaggatc aactatttga agagggaata gaggacatag
      661 atggaattaa cttttataat cctaccatag cagaagaaag ggacataagt agggtccatt
      721 ttgcagtacc agatgtgaag tctagggtta cccagtccca tcttatttca gttggtgggg
      781 ccatcaaggc ctttcaaaag gtaatggagg gagaagtaga taaatccttt gccctggtta
      841 gacccccagg tcatcatgca catcgggtgg tctatggtga taggggcttt tgcatagtta
      901 atgtggaagc agttatgtta gaaaaaatca ggcaggagta taaggattta cgggtagcca
      961 tagtagatac tgattgtcac catggtgatg gtacccaaga catatattgg aatgataagg
     1021 ataccctctt tatctctttt caccaggatg gcagaaccct ttatccaggt actggccata
     1081 ttgatgaatt tggtgggcct aaagcctatg gatacaatgt aaatatccca ctacctcccg
     1141 gtacaggtga ggaaggcttt ctctatgtcc ttgatcatgt ggttttacct attttagatg
     1201 aatatcagcc agatatcata ataaattcag ctggccagga taaccattat actgatccaa
     1261 ttactaatat gaattttaca gcccagggat atgctaagct aaaccacaag cttaatcctg
     1321 atattgcagt tttggaaggt ggttattcca tagaaggtgc tctgccttat attaatctag
     1381 gtataatttt ggccatggct ggcattgatt attctaatgt taaagagcct gactatgatc
     1441 ctgataagct aagacaaact gatgatatta ctgaatatat aaaacaaatt tcagaacatg
     1501 tatataaaca gtggagaaac aaagaagagc ttggtcataa gctttttaaa ggcaaggact
     1561 gggttaaaag caataggcgg atatattatg atacagacaa cattatggaa aaacaaaccc
     1621 aaagctttaa ggtttgtaat aagtgttcag gcttaaacac cattgaatca agaaacaata
     1681 gaggtcatag aatctttgcc ataactattc ctagaaaggc ctgttctgcc tgcatagacc
     1741 aagcttatag cttatataaa aactctaatt ctacctacac caacctgtat ttgcaagata
     1801 gggtacatga caaatattat gctaagtagg aaatataatt gtttattgtt agttataagt
     1861 aatatgaaaa gtgatgcaca aaaaataaat ctggaaaatg gtttatccat tttccagatt
     1921 ctctttatat cttctctagt tttattcctg tcatttggcc gttaagatct tgcttttcca
     1981 attcttcatc cactcttatg atggattttg ctagagtttc agttttgata aagtcttcat
     2041 gctcttttat ggcctctttt atttcatcag agccactata atagatattt atcttatcca
     2101 agacctcata accattattc ttcctcattt gctgtacctt ggagataaat tccctggcat
     2161 atccttcatt aattagctct ggtgttaagc tagtatctag tatcacaaat aagttgtctt
     2221 ccattactac attaaatcct tcc
//
LOCUS       DATGSI010000609         1046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_703644, whole genome shotgun sequence.
ACCESSION   DATGSI010000609 DATGSI010000000
VERSION     DATGSI010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703644"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VK031_09370"
     CDS             <1..553
                     /locus_tag="VK031_09370"
                     /inference="COORDINATES: protein motif:HMM:NF017328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ger(x)C family spore germination C-terminal
                     domain-containing protein"
                     /protein_id="HLS54171.1"
                     /translation="HMPVGIAGNNEVIVSGAGVFKNYKLVGYLNEMENRDVAILNGDL
                     VQDGLDTQYEDSNLSLLIANLKSKRKIITGDEDIKVIFNVQVEGQIHEFILDDNDKID
                     SDLIIKDMEKVLCKEMEKGLNKTIYKLQRELNADVIGILEHLYRFHPSLWEQVKDDWD
                     SIFPQLDIKVNIDLNIRRRGLTT"
     gene            complement(573..>1046)
                     /locus_tag="VK031_09375"
     CDS             complement(573..>1046)
                     /locus_tag="VK031_09375"
                     /inference="COORDINATES: protein motif:HMM:NF021093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HLS54172.1"
                     /translation="QKTKLLVRDEILRVMEEKLKDAQSLAESKKIIEKNLSLIKSVSQ
                     TKLRELGQDLDVRVSLHRENFPIRKYGSLVLPQGEYESLVVELGKAQGENWWCVMFPP
                     LCFIDITQSTRNMSSQEDNNFFIDETQPFKLKSIIGDWFKNIFNKGSKDNQSLET"
BASE COUNT          346 a          157 c          200 g          343 t
ORIGIN      
        1 ccacatgcca gtaggtattg ctggtaataa tgaggtaata gtatctggtg caggtgtttt
       61 taaaaattac aagctagtag gttatctaaa tgaaatggaa aatagggatg tggccatatt
      121 aaatggagac ctggtccagg acggcttaga tactcaatat gaagattcaa atctttcctt
      181 acttatagcc aatttaaagt caaaaaggaa gataattaca ggcgatgaag atataaaggt
      241 tatatttaac gtacaagtgg aaggacagat ccatgaattt atcctagatg acaatgataa
      301 aatagatagt gacttgatta taaaagatat ggaaaaggta ctgtgtaagg agatggagaa
      361 aggattaaat aaaacaattt ataaactaca aagggaacta aatgcagatg ttattggaat
      421 tttagaacac ttatataggt ttcatccaag cctgtgggag caggtaaaag atgattggga
      481 tagtattttt ccccaattag atattaaggt aaatatagat ttgaatatta gaagaagggg
      541 actgacaacc taatgccagg gaggatctgg cattaggtct ctagggattg attatcttta
      601 ctccctttat taaatatatt tttaaaccag tctcctatta tggattttaa tttaaaaggt
      661 tgggtttcgt ctatgaaaaa gttattatcc tcttggctgg acatatttct tgtagactgg
      721 gttatatcta taaaacacag aggtggaaac ataacacacc accaattttc gccctgtgcc
      781 ttacccaatt ctaccactaa agattcgtat tctccttgag gtaaaactag ggagccatat
      841 tttcttatag ggaagttttc tctatgaaga gataccctta catccaaatc ttgacctaac
      901 tctcttaatt ttgtttggga gactgatttt attagggata agttcttttc aataatcttc
      961 ttactttccg ctaaggattg ggcatctttt aacttttctt ccatgaccct taaaatctca
     1021 tctcttacca gcaattttgt tttttg
//
LOCUS       DATGSI010000610         1108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_88677, whole genome shotgun sequence.
ACCESSION   DATGSI010000610 DATGSI010000000
VERSION     DATGSI010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88677"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VK031_09380"
     CDS             <1..854
                     /locus_tag="VK031_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLS54173.1"
                     /translation="EIFKTGISMSKYIASNPDNYQEKILQAQEKLERLKMEKAYLLTE
                     SNIPLDYMEIKYDCQGCEDTGYLSNGDKCNCLRQALVSKAYKMSNLGNILEKENFQNF
                     DITIFSDEPFEGEELTPRENMRDLVGIAEGFVNNFDEDNGENLLFYGTTGLGKTYLCN
                     CIAKSLLDKNKIVVYQTAFTILEILENRRFGRGDRELGDYQYNLLFDSDLLIIDDLGT
                     EISNTFTNAEIFNIVNTRIIAGKKTIISTNLTPNEISQIYTDRIFSRIFEKFIPLKFY
                     GKDLRWE"
BASE COUNT          429 a          140 c          203 g          336 t
ORIGIN      
        1 aagaaatatt taaaacggga atcagtatgt ctaaatacat agcaagcaat ccagataatt
       61 atcaggaaaa aattcttcaa gcccaggaaa aattggaacg attaaagatg gaaaaggcct
      121 atcttttaac ggaatctaat attcctctag actatatgga aataaagtat gattgccaag
      181 gctgtgaaga tacaggctac ctatccaatg gagataaatg taattgccta agacaggccc
      241 ttgtatctaa ggcctataag atgtctaact tgggaaatat tttggaaaaa gaaaacttcc
      301 agaactttga tataaccata tttagcgatg aaccctttga aggggaagaa ttgactccaa
      361 gggaaaatat gagggatctg gttggaatag ctgaaggatt tgtaaacaat tttgatgagg
      421 ataatggaga aaatctattg ttctatggta caactggtct tggaaaaaca tatctttgta
      481 actgtatagc caaatctctg ttggataaaa ataaaatagt ggtttatcaa acagccttta
      541 ccatactaga aattttagaa aataggagat ttggtagagg tgatagggag cttggtgatt
      601 atcaatacaa tcttctattt gattcagatc ttttaataat agatgattta ggtacagaaa
      661 tatctaatac ctttacaaat gcagaaatct ttaatattgt aaacacaaga attatagctg
      721 ggaaaaaaac aattatatca actaatttaa caccaaatga gatctcgcaa atatacacag
      781 ataggatatt ttccaggata tttgaaaaat ttatcccctt aaagttttat ggaaaggact
      841 taagatggga ataataaaaa aacctgtaga gaatctacag gtttttttat gtattaatgt
      901 tataatacta ataattaata aaataagggt ggtaaggcat ggaaaaccaa gaagctaagg
      961 ataagataaa ggattattta gtcaaggtat taaatggtat ggccctaggc ctgttttctt
     1021 cattactaat aggacttatt ataaaacaaa taggaacttt atttaatttg gacttattaa
     1081 ttatttttgg agatatggcc caaaaact
//
LOCUS       DATGSI010000611         1277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_322077, whole genome shotgun sequence.
ACCESSION   DATGSI010000611 DATGSI010000000
VERSION     DATGSI010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322077"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VK031_09385"
     CDS             complement(<1..311)
                     /locus_tag="VK031_09385"
                     /inference="COORDINATES: protein motif:HMM:NF021056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase N-terminal domain-containing protein"
                     /protein_id="HLS54174.1"
                     /translation="MTKKDLKGRLTLPSEENFYKETLEMLKKLKADAIRDSDGTKLPD
                     EIKDIEDALIYTTYFTVRDINDFAKDNMEEAIRLYLSSEFKTATDTSLTIDLMEDYFD
                     E"
     gene            complement(311..1186)
                     /locus_tag="VK031_09390"
     CDS             complement(311..1186)
                     /locus_tag="VK031_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004839240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLS54175.1"
                     /translation="MKDNSKFFKIFIYFCLVVMALSILIPVIWVFMSSLKTNPEFYQN
                     PWKLPASFYLENFKDAWQAANMGDFFLNSVIVTFLGLVILLLIALPAAYILARMEFKG
                     KSIVQTILKAGLFVNLSYIVIPIFMLLRDVDKMVNKTGILSGGFMLNNRLVLAIIYAA
                     TAIPFTVYLLTNFFSSISKSYEEAAYIDGAGYFRTMVDIMIPMAKPAVITVVLFNFLS
                     FWNEYILALTLMTDESKKTLPVGLINLQQAARGAANYGRLYAGLVIVMIPTLIMYILV
                     QKQLTEGMMVGGDKG"
BASE COUNT          401 a          227 c          205 g          444 t
ORIGIN      
        1 tgctcatcaa agtaatcttc catcaaatct atggtaagac ttgtatctgt agctgtctta
       61 aattcgcttg aaagatataa tcttatggct tcttccatat tatctttggc aaagtcattg
      121 atatccctta ctgtaaaata agttgtataa ataagggcat cttcaatgtc tttaatctcg
      181 tcaggaagct tagttccatc tgaatctctt atggcatctg ccttaagctt tttgagcatt
      241 tctaaagttt ctttataaaa attttcttca cttggaaggg ttagccttcc ctttaaatcc
      301 ttttttgtca ttatcccttg tctcctccta ccatcattcc ttctgtaagt tgtttttgta
      361 caagtatata cataataagg gttggtatca ttactattac aagtcctgca taaagcctac
      421 catagtttgc agcccctctt gctgcttgtt gtaggtttat aagtcctaca ggtagggttt
      481 ttttgctttc gtctgtcata agggttaagg ctaggatata ttcattccaa aaagacaaaa
      541 agttaaataa tactacagtt ataactgcag gttttgccat tggaatcatt atatctacca
      601 tggtcctaaa atatcctgcc ccatctatat aggctgcctc ttcataggat ttagaaatag
      661 atgaaaaaaa gtttgtcaaa agataaaccg taaaaggaat ggctgttgct gcataaatta
      721 tggctaaaac tagcctatta tttagcataa aaccacctga taggattcct gttttattga
      781 ccatcttgtc aacatccctt aaaagcataa agattggaat aactatataa gaaaggttaa
      841 caaaaagtcc tgccttaagt atggtttgaa ctatactttt tcccttaaat tccatcctag
      901 ctagaatata ggctgctgga agggctatta gtaaaagtat tacaagtcct aaaaatgtaa
      961 ctatgacaga atttaggaaa aaatctccca tatttgccgc ctgccaggca tctttaaagt
     1021 tttctagata aaagcttgct ggaagcttcc atggattttg gtaaaattct ggatttgtct
     1081 taagcgatga catgaaaacc catataactg gtataaggat tgacagtgcc ataactacaa
     1141 ggcaaaagta tataaatatt ttgaaaaatt tagaattgtc tttcatatct ttctcctagt
     1201 attggattaa atctcttttt gttatcctat ggactatggc tgataggata aatgagaata
     1261 aaaatactac aactcct
//
LOCUS       DATGSI010000612         1159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_231281, whole genome shotgun sequence.
ACCESSION   DATGSI010000612 DATGSI010000000
VERSION     DATGSI010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231281"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1159
                     /locus_tag="VK031_09395"
     CDS             <1..>1159
                     /locus_tag="VK031_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HLS54176.1"
                     /translation="SQYALADMILSQPYLKSGTVLFVNASLDSNSLEDKRYAGLEGGS
                     IPAGVNKDKVIYYKTREATLNAVDMGQADYGYGNAYSVSYYTLKNGYKNIVTIPRGIE
                     TREYCIGLVKEDYILLSIINKAIASMDETAVQTLILDMTSKIDRKITLPMILDLYDLE
                     IFLGSMLIIGLLILGIATNVRAKHHLKIENQRYKILSHISNEYLYEYNFKSDRLTLSE
                     SLSHIFLNRQDLDLFKARLKEDLIKQNFKEIIPRLDLPLSKSERRIFKGINSKIYDDR
                     NNIYSVIGKLVDVTEETAEKDALITKAQVDGLTGLYNASTTKELILEKIEARDNLQKD
                     VFMIIDGDRFKEINDTFGHLEGNRVLKKIAQAMRLTFRQTDIIGRVGGDEFA"
BASE COUNT          428 a          163 c          232 g          336 t
ORIGIN      
        1 catctcaata tgcactggca gatatgatcc tatcacaacc atacttaaag tcggggaccg
       61 ttttatttgt aaacgcctcc ttagactcca atagcctaga agataaaaga tatgcaggct
      121 tagaaggggg atctatacct gctggagtta ataaggacaa ggtaatttat tataaaacta
      181 gggaggctac cttaaatgca gtggatatgg ggcaggctga ttatggttat gggaatgcat
      241 attctgtttc atattatacc ctgaaaaatg gttacaaaaa tatagtgact ataccaagag
      301 gaattgaaac tagagagtac tgtatagggc tggttaaaga ggattatatc ctactttcca
      361 taattaataa ggcaatagct tctatggatg agacggctgt acaaacccta atattagata
      421 tgacatctaa gatagacaga aaaattaccc tccctatgat attagattta tatgatctag
      481 aaatattttt aggttccatg ttaataatag gcctattaat attggggata gccactaatg
      541 tgcgggcaaa acaccatctt aaaattgaaa atcaaagata taaaatactg tcccatattt
      601 ctaatgaata tttatatgag tataatttta aatccgatag actaaccctg tcggaaagct
      661 taagccatat attcttaaat aggcaggatt tagacctatt taaggctaga ttaaaggagg
      721 atttaattaa gcaaaatttc aaggaaatta ttcctagact agatttgccc ttaagcaaat
      781 ctgaaagaag gatttttaaa gggattaatt ctaaaatcta tgatgatagg aataacatat
      841 attctgttat tggtaaactg gtagatgtaa ctgaagaaac ggcagaaaaa gatgccttaa
      901 ttactaaagc gcaagtggat ggcctgacag gtctttataa tgcaagcaca actaaggaac
      961 ttattttaga gaaaatagaa gctagagata atttacaaaa agatgtattt atgataatag
     1021 atggggatag atttaaagaa ataaacgata cctttggcca tttggaagga aacagggtac
     1081 tgaaaaagat agcccaggcc atgagactta cttttagaca aacagatata attggtcggg
     1141 taggtgggga tgaatttgc
//
LOCUS       DATGSI010000613         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_231304, whole genome shotgun sequence.
ACCESSION   DATGSI010000613 DATGSI010000000
VERSION     DATGSI010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231304"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VK031_09400"
     CDS             <1..793
                     /locus_tag="VK031_09400"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HLS54177.1"
                     /translation="VADIVHRGGTILRSSRSDEFETEAGFKKAMNIIRVFGIDGLVIL
                     GGDGSLKGAQELSKAGIPTIGIPCTIDNDLGYTDYTIGFFTAVETVIDAISKIRDTSS
                     SHGRANIIEVMGRHCGDIALYAGFAGGAESIIVPEVPFDMDEVNKKVIQGKNRGKLHH
                     IIVLAEGVGNAYKVAEEVVEKTGVDTRVTILGHIQRGGTPSAFDRILASEMGRKAVEL
                     LIDGESGKTLGIKCNEVISVDIPEALETEKVFNIDMYETTKLLSI"
     gene            805..>2252
                     /gene="pyk"
                     /locus_tag="VK031_09405"
     CDS             805..>2252
                     /gene="pyk"
                     /locus_tag="VK031_09405"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HLS54178.1"
                     /translation="MKKTKIVGTIGPASESEEVLEELFKNGLNVCRLNFSHGDHEEHK
                     IRIDRIKKVREKLNLPIGIMLDTKGPEIRLGNFKEEPVELSQGDIFTLTTRDVLGDKS
                     IVNVSYEGLANDVEVGSIILIDDGLVELEVLEITDGTDIKCRALNSGELKNHKGVNVP
                     NVEINLPAITQKDINDILFGIENGVDFIAASFIRKADDVTEIRRILEENNGHSIDIIS
                     KIENQQGVDNIDEILTVSDGIMVARGDLGVEIETEIMPLVQKELIRKCNAVGKPVITA
                     TQMLDSMIRNPRPTRAEVTDVANAILDGTSAIMLSGETAAGKYPVESVKTMYNIAMKT
                     EESLDYPELLRTRSSTSDVSTTNAISRATCYTAQDLQASAIITATSSGHTSKAISKFR
                     PKPPIIAATTTESVTRKLSLAWGVYPILVPDSGSTDEVISNSVEHAIKNGYAKEGDLV
                     VLTAGIPVGMSGSTNLIKVQTIGKVLLTGTGIG"
BASE COUNT          818 a          316 c          516 g          602 t
ORIGIN      
        1 tgtagcagat attgtacata ggggaggtac tatcttaaga agtagtaggt cagatgaatt
       61 tgagacagaa gctggattta agaaggctat gaatatcata agagtttttg gaatagatgg
      121 cctggtaata ctaggtggag atggaagctt gaaaggtgcc caagagttgt ctaaagcagg
      181 aatacctact attggaattc cctgtaccat agataatgat ttagggtata cagattatac
      241 tataggcttc tttactgcag tagaaacagt aattgatgcc ataagtaaaa tcagagacac
      301 ctcttcttcc catggtagag ctaatattat agaggtaatg ggaagacatt gcggtgatat
      361 agccttatat gctggatttg caggaggagc ggaaagtatc atagttccag aggtaccctt
      421 tgatatggat gaggtaaaca agaaagtaat ccaaggtaaa aacaggggta aacttcacca
      481 tattatagtt ttagctgagg gagtaggtaa tgcttataaa gtagctgaag aagtagtgga
      541 aaaaacagga gtagatacca gggtcaccat actaggtcat attcaaagag gagggacccc
      601 atctgccttt gacaggatac tggccagtga aatgggtaga aaggcagtag aattattaat
      661 agatggtgaa agtggcaaga ccttaggaat caaatgtaat gaggtcataa gcgtagatat
      721 tcctgaagct ttagaaactg aaaaagtatt taatattgac atgtatgaaa ccactaaatt
      781 attatccatt taggaggaat acttatgaaa aaaactaaaa ttgtaggaac tataggacca
      841 gcttcagagt cagaagaggt tctagaagag ttatttaaaa atggcttaaa tgtttgtaga
      901 ttaaactttt cacatggaga tcatgaagaa cacaagataa gaattgatag aataaaaaag
      961 gtaagggaaa aattaaatct acctataggt attatgctgg atacaaaggg accagaaatc
     1021 agattaggaa actttaaaga ggagccagtt gaattatcac agggagatat ttttactcta
     1081 actaccaggg atgtacttgg agataaatca atagtaaatg tgtcctatga gggactggct
     1141 aatgatgtgg aagtaggttc tataatctta atcgatgatg gcttggttga gttggaagtt
     1201 ttggaaatta ctgatggcac tgatataaaa tgtagggccc taaatagcgg tgagttaaag
     1261 aaccataagg gtgtaaatgt acctaatgtt gaaattaatt tgcctgctat tactcagaag
     1321 gatattaatg acatattgtt tggtatagaa aatggagtag actttatagc agcatctttc
     1381 ataagaaagg cagacgatgt tacagaaatt aggagaattc ttgaagaaaa caatggacac
     1441 agcatagata taatctcaaa gattgaaaac caacagggtg tagataatat agatgagatt
     1501 cttacggttt ctgatggaat tatggtagct agaggagatt taggtgtaga gatagaaact
     1561 gaaataatgc ctctagtaca aaaagaatta attagaaaat gtaatgctgt aggtaaacct
     1621 gtgattacag ccacccagat gttagattcc atgataagaa atccaaggcc aactagggct
     1681 gaagtaactg atgtggccaa tgctatactt gatggaacca gtgcaattat gttgtcagga
     1741 gagacagcag caggaaaata tccagtagaa tcagtaaaga ctatgtataa tatagctatg
     1801 aaaacagaag aatcactgga ctaccctgaa ttattaagga ccagatctag tacatctgat
     1861 gttagcacaa caaatgccat aagcagggct acctgttata cggctcaaga tttgcaggcc
     1921 tctgccataa taactgcaac ttcctcagga catacatcaa aggcaatatc caaatttagg
     1981 cctaagccgc ctattattgc agctacaaca actgaatcag taacaagaaa gctatctttg
     2041 gcctggggag tatatcctat attggtgcca gatagtggtt caactgatga ggttatttcc
     2101 aattctgttg aacatgccat taaaaatgga tatgcaaaag aaggagattt agttgtacta
     2161 acagcaggaa taccggtagg aatgtctgga tcaactaatc taattaaggt gcaaaccata
     2221 ggaaaagtac tacttacagg tactggaata gg
//
LOCUS       DATGSI010000614         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_418751, whole genome shotgun sequence.
ACCESSION   DATGSI010000614 DATGSI010000000
VERSION     DATGSI010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418751"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VK031_09410"
     CDS             <1..1085
                     /locus_tag="VK031_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLS54179.1"
                     /translation="SQTFREAMNNSCNVAFINIARQLGRDKLYSYLKAFGFGKKTGID
                     LLGEQTGLIPGKVESIREINLATMSYGHGIAVTPIQLLNATAAIANGGDLMKPRLVKE
                     LIDSEGNIVEKLEPEVVRKVISKETSETMLSLMETVVTDGSGSRAHVPGYRVGGKTGT
                     AQKIIDGVYAQGKYIGSFVAVAPIEDPKIVLLVIVDEPSGQYYGGSIAAPVAKDLLEE
                     IFNYLEIPPVYDEEEDEELGMVTVPDLRNKPVEEAGRILTELGLRYTTEFMEITDNSK
                     ILDQFPKAGVEVQKGSIIDLYVDIIPENRVSMPYLVGKSKEEVIKILDEMNLQYTLHG
                     QGNAVVQDPMPGEEVKQNTKIVIEFE"
     gene            1162..2610
                     /locus_tag="VK031_09415"
     CDS             1162..2610
                     /locus_tag="VK031_09415"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HLS54180.1"
                     /translation="MDLRDIIGSYPILEIKGPRDINISKLTHDSRKVNKGDLFIAQRG
                     FTYDGHEFITDAINKGALAIVCEEDFTYEKITKIKVLDSTDALGYFSSRFYKLPWARL
                     GMVGITGTNGKTSIAHYLRYIFGQEKIKTAILGTMGAIINKSQIELINTTPDALLIQE
                     IVNEMVRKKVKYCFMEVSSHALDMKRVKYMDFDMAIFTNLSNEHLDYHKNIDNYFQAK
                     SRLFNKTKKNIINLDDPYGRRLIKEIDGKIPTFTYGLGKKADAYASNISYTNNTSNFT
                     LNILGERAEVKLNALGQFSIYNALAASLCAYLYGIDIKTIKNALKSMETIKGRTEFIP
                     NKSGINIVIDFCHTPDGLREVLLALKKITKGNIIVVFGAGGNRDPDKRPEMGEVVGRL
                     ADLAIISSDNPRFENPETIINDIIIGVKKSQADYVKIIDRREAIEYALRLAKTGDTIL
                     LAGKGHEEYMVIEDKKYPFSERELVLNILNNE"
     gene            2638..>3160
                     /locus_tag="VK031_09420"
     CDS             2638..>3160
                     /locus_tag="VK031_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HLS54181.1"
                     /translation="MIDYIDIIRTIMIAFFISLILGPIIIPMLKLLNIGQSIREEGPK
                     SHMAKSGTPTMGGIIMVIALIITVISSGLMNRDMYVLLLSTLGFGLIGFVDDYIKVVK
                     KRNLGLKAYQKLIGQVILAVILAIYQSQTSILGTQVIIPFVENRYLDLGVLYIPFIAF
                     VVVGTVNSVNLTDG"
BASE COUNT         1141 a          429 c          673 g          917 t
ORIGIN      
        1 gtagccaaac ctttagagag gccatgaata attcatgtaa cgttgctttt atcaacattg
       61 caagacaatt aggcagggat aaactttatt catacctaaa ggcctttggt tttggtaaaa
      121 aaacaggtat agatctttta ggggaacaga caggcctaat acctggaaag gtggaatcca
      181 taagggagat aaatctagct accatgtcct atggacatgg aattgcagtt acacctatac
      241 agcttttaaa tgccactgct gccatagcca atggtggaga tctgatgaaa ccaaggctag
      301 ttaaagaatt gatagatagt gaaggaaata ttgtagaaaa attggaacct gaagttgtaa
      361 gaaaggtgat ttctaaagaa acatcagaaa ccatgttaag cctgatggaa acagtagtaa
      421 cagacgggtc aggctcccgg gctcatgtac caggctatag ggttggaggt aagacaggaa
      481 ctgcgcaaaa gattatagat ggtgtatacg cacaaggaaa atatataggt tcctttgttg
      541 ctgtagctcc tatagaggat ccaaagatag ttcttctagt aatagtggat gaaccaagtg
      601 gccaatatta cggtggctcc atagcagccc cagtggccaa ggatttgctt gaggaaatct
      661 ttaattacct agagatacct ccagtatacg atgaagagga agatgaagaa ttaggaatgg
      721 tgacagtacc tgatctaaga aataagccgg tagaagaggc tggaaggatt ttgacagagt
      781 taggcttaag atatacaaca gagtttatgg aaataacaga taactctaag attctagacc
      841 aatttcctaa ggcaggagtg gaagttcaaa aaggttccat tatagaccta tatgttgata
      901 taataccgga aaacagggtc agcatgcctt atttagtagg caaaagtaag gaagaagtaa
      961 taaaaatact tgatgaaatg aatttacagt atactttgca cgggcaaggc aatgcagttg
     1021 tccaggatcc tatgccaggt gaagaagtta aacaaaacac caaaatcgta atcgaatttg
     1081 agtaactaat agatgaacta atataaggta actatttata gttgccttat aattaattat
     1141 agtctattag gaggtggcat attggatcta agggatatta taggaagtta tccgatcctt
     1201 gaaataaagg ggcctagaga tattaatatc tctaaactga cccatgattc caggaaagta
     1261 aacaagggag atttatttat agctcaaagg ggttttactt atgatggtca tgaatttata
     1321 actgatgcca ttaataaggg ggctctggca atagtttgtg aagaagattt tacctatgaa
     1381 aagataacta aaattaaagt tttagattct accgatgcct tagggtattt ttccagtagg
     1441 ttttacaaat tgccgtgggc caggctaggg atggtaggta ttacaggaac aaatggaaaa
     1501 acaagtatag cccattattt gagatatatt tttgggcaag aaaagataaa aacagctatc
     1561 cttggaacta tgggggctat aataaacaaa tctcagatag agttaattaa tactacacca
     1621 gatgctttat tgatacaaga aattgtgaat gaaatggtaa ggaagaaggt caaatattgt
     1681 tttatggaag tctcctccca tgccctagat atgaaaaggg tcaaatatat ggactttgat
     1741 atggccatat ttactaactt atccaatgaa catctagact atcataagaa tattgataat
     1801 tattttcaag ctaaatctag actctttaac aagactaaaa agaatattat taatttagat
     1861 gatccctatg gaagaaggct tattaaagaa atagatggga aaattccaac atttacatat
     1921 ggattaggta agaaggcaga tgcttatgcc agtaatataa gttatactaa taatacttct
     1981 aactttacct taaatatatt aggggagagg gcagaggtaa aattaaatgc cttaggccaa
     2041 tttagtattt ataatgccct agcggccagc ctctgtgctt acctgtatgg aatagatata
     2101 aaaactatta aaaatgcttt aaagagcatg gaaactatta agggtaggac tgaatttatt
     2161 ccaaacaaaa gtggtataaa tatagttata gatttttgtc atacacctga tggtttaagg
     2221 gaagtgttat tagccttaaa aaagataacc aagggaaata taatagtagt ttttggggct
     2281 gggggtaata gggatccgga taaaaggcct gaaatggggg aagtggtagg acggctggca
     2341 gaccttgcca taataagttc tgataatcca agatttgaaa acccagaaac aataattaat
     2401 gatataataa taggggtaaa gaaaagtcag gcagactatg ttaaaataat agatagaaga
     2461 gaggctatag aatatgccct aaggcttgca aagaccggtg atactatact attggcaggt
     2521 aagggacatg aagagtatat ggttatagaa gataaaaaat acccttttag tgagagggag
     2581 ctagtactaa atatattaaa taatgaataa tgtaatttac ttgagaggag taacttaatg
     2641 attgattata ttgacataat acggactatt atgattgctt tttttatatc cttaatttta
     2701 ggacctatta taattccaat gttaaaactt ttaaatattg ggcaaagtat tagggaggaa
     2761 ggacctaaat cccatatggc taaatcaggc acaccaacta tgggtggaat aataatggtt
     2821 atagccctaa taattactgt tataagttca ggtttaatga acagagacat gtatgtatta
     2881 cttttatcaa ctttaggttt tggattaata ggttttgttg atgattatat taaggtagtt
     2941 aaaaaaagga atttaggctt aaaggcctat caaaaattaa taggccaagt aattttggca
     3001 gtaattcttg caatatacca atcccagact tctattttag gtacacaggt aataatcccc
     3061 tttgtagaaa atagatactt agatctggga gtcttgtata taccctttat tgcttttgtg
     3121 gtagttggaa ctgtaaatag tgtaaatttg acagacggtt
//
LOCUS       DATGSI010000615         1482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_609788, whole genome shotgun sequence.
ACCESSION   DATGSI010000615 DATGSI010000000
VERSION     DATGSI010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609788"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VK031_09425"
     CDS             complement(<1..202)
                     /locus_tag="VK031_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HLS54182.1"
                     /translation="MTFLKETSMLKELRLLQHIESYPNTTQKQIAKAIDSAPSMVNAY
                     IDNLEKKQYLKRVYKSAKNVSYH"
     gene            complement(331..1221)
                     /locus_tag="VK031_09430"
     CDS             complement(331..1221)
                     /locus_tag="VK031_09430"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HLS54183.1"
                     /translation="MKFTKKRLALALTLLMLLFTLTGCGGGKDTIIKVGGKNFTEQYI
                     TSHMLSILIEENTDLKTEIKDNLSATVIFEAIKSDEVDLYVEYIGSGLSNLHMELEGS
                     TEEQFEAVKTRFADELNITWLDPLGFNNTYAMVVTPETAEKYKLETVSDMAKVADDLV
                     LGCTQIFTERADGVPGLTSHYDFEFKDIKAMDAALMYQALVQDDVDVISGFLTEGRIA
                     AHDLVVLDDDKDFFPPYDSSIIVRNEVLEKHPELEEVLNKLTGKIDNNTMAELNAAVD
                     LDKRDPEEVAREFLEKEGLI"
     gene            complement(1234..>1482)
                     /locus_tag="VK031_09435"
     CDS             complement(1234..>1482)
                     /locus_tag="VK031_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLS54184.1"
                     /translation="QALSVIMAGVRIATVINIGTTTIAAYIGGGGLGDLIFKGIQMYR
                     NDMILAGAIPAALLAIIADKLLGYVELKLTPRGMKKNI"
BASE COUNT          393 a          315 c          232 g          542 t
ORIGIN      
        1 tatgatagga tacatttttt gcagacttat agaccctctt tagatattgc tttttctcta
       61 aattgtctat ataggcattt accatggagg gtgcactgtc tatggccttg gctatttgtt
      121 tttgtgtagt atttgggtaa ctttcaatgt gctgcaaaag ccttaattcc ttcagcattg
      181 atgtttcctt taggaatgtc atcctacttc cccttcttta ttcgacgtct gaatgattat
      241 agtttaccac attattccac atgggtaaac agattctaaa aaaagatgtc aaaagtccca
      301 gtttttctgg gacttttctt ttaggtcagc ttatattaat ccttcttttt ctaaaaattc
      361 tctagccacc tcttctgggt ctcttttatc taggtctact gctgcattta attctgccat
      421 tgtattgttg tctatttttc ctgttagttt atttaatacc tcttctagct ctggatgttt
      481 ttcaaggact tcatttctaa ctatgattga agaatcatag ggtggaaaaa agtctttgtc
      541 atcatctagt actactaaat catgggctgc aatcctacct tcagttaaaa agccactgat
      601 tacatccaca tcatcctgga ccagagcctg atacattaag gctgcatcca tagcctttat
      661 atctttaaat tcaaaatcat aatgggaggt aagtccaggt accccatctg ccctttcagt
      721 aaagatttgt gtacagccaa gtaccaaatc atctgccacc ttagccatat ctgatacagt
      781 ttctagcttg tacttttcgg cagtctctgg tgttaccacc atggcatagg tattattaaa
      841 tcctaaagga tctagccagg taatgtttaa ttcatctgca aatctagttt ttactgcctc
      901 aaattgctct tctgtgctgc cttccaactc catatgaaga tttgacaatc ctgagccaat
      961 gtattccaca tataggtcta cttcatctga ctttatggct tcaaaaatta cagttgcaga
     1021 taaattatcc ttaatttctg ttttcagatc tgtattttcc tctattaata tacttagcat
     1081 atgggatgtt atatattgtt cagtaaaatt tttcccacct actttaataa ttgtatcctt
     1141 cccgccacca catcctgtta aggtaaatag tagcattaaa agagttaaag ctaaagctaa
     1201 tcttttctta gtaaatttca taataatcct cctttagata tttttcttca ttcctctagg
     1261 tgtaagttta agttccacat agcctaatag cttgtctgca ataattgcca gcagggctgc
     1321 cggtatggct cctgccagga tcatatcatt tctatacatt tggattccct taaagattaa
     1381 atctcccagg cccccgccgc ctatataggc tgcaattgtg gtggtgccta tatttatgac
     1441 tgtagctatc ctaactccag ccataattac agacaaggcc tg
//
LOCUS       DATGSI010000616         7472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_273296, whole genome shotgun sequence.
ACCESSION   DATGSI010000616 DATGSI010000000
VERSION     DATGSI010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273296"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="VK031_09440"
     CDS             complement(<1..1413)
                     /locus_tag="VK031_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HLS54185.1"
                     /translation="MNFKDWEASLAEGKNPCYKCRQSGRDEAGDNLFYYGEGEGYHCF
                     SCGWTKPSDDFLEELEELEPQEYEYMATEWNEELYNQIKENSGVDSKNYRGIRTDISA
                     TLGVRYEYDTETGEVAKTFYPVTKGCLEKEIKDSLCGLKIREHPKKFYSVGSAGSDCD
                     LFMQWKFRTHRGILVITAGEIDAMSAYQMLYDMHQRSGNAKKYDEIAVVSSTIGEGGT
                     AKQVRQQYQWVDQFSKIIVLMDNDEAGRKATEEVVNVLPKGKAFVADLRYKDINEYLE
                     RGDEQAFINDFWGHRPYTLDGVKSSFDGFLEIEEEILKPRIPLPKYMHKLQENTGGGL
                     ARGAIHNIIAATGVSKTTHLRQIVYHMIMYTDLKPTIISLEETAARYNLELLQLHAGE
                     NFTFGKTGEEIIDYISTDRMVKLRQELIQNELGEPRYYIIDERSGDIKSIEKQMDLMY
                     NKFGSEVFVIDVLSDLLRGSI"
     gene            complement(1501..2607)
                     /locus_tag="VK031_09445"
     CDS             complement(1501..2607)
                     /locus_tag="VK031_09445"
                     /inference="COORDINATES: protein motif:HMM:NF021059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase"
                     /protein_id="HLS54186.1"
                     /translation="MHLIEYIKSNGLDSLKEDLGIKIREYAGGLSVFNYYQINSPKTH
                     PVVMECRGVALDEDLNIIRRPFDRFFNLGEAECSQPDIDWESSYVYEKPDGTLMILYY
                     WQGDWHVGTRGTAFGENGVGTSGITFVGLFEKALGVGSPVEMQEACGELDKDFTYMFE
                     LTSPHNRVVTPYESTSITLIGKRHTQSGEYADINEEKGIEGVKIAKRYPLGSPEGVLE
                     AAKSLPPMEEGYVVYNSLMQPVVKVKNPAYVALHRIRGNGIKNSPRSVAELVVLREDQ
                     EYLSYFPEDREIIDKAANAWIWLYKDAQSLYEKYKDVESQKEFALSVKVSPMSSVLFN
                     ARKEGKTFPEALGDMPLNFKVKVLLENWDIDKQE"
     gene            3107..3340
                     /locus_tag="VK031_09450"
     CDS             3107..3340
                     /locus_tag="VK031_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54187.1"
                     /translation="MSIGKSIMPDISTVEVEEKYFKRILKDHLLLEMLISGGVERWTY
                     WDSALDTFNRLKESYVSTAMKDNVVEKRVYKRK"
     gene            3507..3935
                     /locus_tag="VK031_09455"
     CDS             3507..3935
                     /locus_tag="VK031_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54188.1"
                     /translation="MCKDENTTSRGVFEKMLEGEGENLTFEQKQKIFYKKISTSIGEV
                     VEATEMGGVFGKMFDSEEYDDYTFSQKEELFCKRVGILAENIAKTYMENLEIEFIPEE
                     DNTYSCILKIDCNHFKDLINKGLEAMLQGDDLEELGKIKE"
     gene            4112..4453
                     /locus_tag="VK031_09460"
     CDS             4112..4453
                     /locus_tag="VK031_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54189.1"
                     /translation="MKHVFVFGSNLAGRHGKGAALFARKHYGAIYGQGVGMQGHSYAI
                     PTKDYNLRTLPLATIEGYVEEFKSFTREHPDKLFIITPIGTGLAGYSKKDIWPMFKGV
                     GSNCVFSHTWC"
     gene            4474..5499
                     /locus_tag="VK031_09465"
     CDS             4474..5499
                     /locus_tag="VK031_09465"
                     /EC_number="6.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase (ATP)"
                     /protein_id="HLS54190.1"
                     /translation="MGRKLVSIRTIDEVKPIEGADRIEACRVGGWWVVGKKGEYKVGD
                     KAIYCEIDSWIPHELAPFLSKNREPREYGGIRGERLSTVKLRGQISQGLLLPLSVLRD
                     YAVPDWAEGRDLSAFLGVIKWEPPIPAVLSGEARGVFPNNISRTHQERVQNLGDGDHV
                     GWHLVTEKLDGASMTAYLHDGEFGVCSRNVNLREDSSNTYWKVARDLYLENVLNRLCE
                     TTFSSEGYAIQGELVGEGIQGNPYGIKGHDFYVFDIWDIKKQAYIPFNVVMFLLQGTG
                     LKNVPVVGKRFLDRVNSQEILKDVDGLKSVVNPKVLAEGVVYKSLERGNKSFKAISNE
                     WLLRGRR"
     gene            complement(5753..6106)
                     /locus_tag="VK031_09470"
     CDS             complement(5753..6106)
                     /locus_tag="VK031_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54191.1"
                     /translation="MSENNCSTTSAAKSRVHDLSNKFDKISGKVVTIGVSVLIALMSM
                     LYTDMKDRVNKMEERVTFLYNDKLSRSEFLDTMREIKEDMLLSRKETDKQIQSVKTEI
                     IERLDLILQYNRDRR"
     gene            complement(6253..6642)
                     /locus_tag="VK031_09475"
     CDS             complement(6253..6642)
                     /locus_tag="VK031_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54192.1"
                     /translation="MLSFLKSHTIFIGVILVCVVVMSVMYATLSKQKATINSLSYDVM
                     VLEGTVVSLEHTLDNIKQLRQIENAELEKSREKFENLESANNKNINRVEEVYKNEEAV
                     DSPLPDDISRLLNEACAEVRGSPCPPP"
     gene            complement(6642..7253)
                     /locus_tag="VK031_09480"
     CDS             complement(6642..7253)
                     /locus_tag="VK031_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HLS54193.1"
                     /translation="MGVKRKLTKRLAALGVSVAAALSGGYLIYPYEGSVTNKDGLHTV
                     YLDPIGIPTACWGQTGRDLYGKPIQMGATYTEDECIQMLANTLKSFEQQINQMTKNNY
                     ASDWQKAALISFAYNSGTGNFQRSTLRSYILAGDHGSACDQLSRWVYANKKKLRGLVL
                     RRGEEREWCLGNVDDAAAIGTYYEIMEKNMDYFKKYRMGEGSE"
     gene            complement(7275..>7472)
                     /locus_tag="VK031_09485"
     CDS             complement(7275..>7472)
                     /locus_tag="VK031_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54194.1"
                     /translation="NWVQNRQDRAISYIFQKGIPEWDESTEYIAGKSFIQHEGSLYRA
                     STTNTGKRPDESPNDWDVVFS"
BASE COUNT         2107 a         1614 c         1473 g         2278 t
ORIGIN      
        1 gatagacccc ctcagcaaat cacttaatac atctatgaca aacacctcac ttccaaactt
       61 attatacata agatccattt gtttctctat gctcttaata tctccacttc tctcatcaat
      121 aatgtaatat ctaggctccc ccagctcgtt ctggataagt tcttgcctta gttttaccat
      181 tctatctgta ctaatgtaat caataatctc ttccccagtt ttgccaaatg tgaagttctc
      241 gccagcgtgc aactgcagta gctctaggtt gtatctagct gcggtttctt ccaaggagat
      301 gattgtaggc ttcagatctg tatacataat catgtgatac acaatctgcc tcaaatgtgt
      361 agtcttagaa accccagtag cggcaattat attgtgtata gcccctctgg ccagcccccc
      421 tccagtattt tcttgcaact tatgcatata cttgggcaag gggattctag gtttcaaaat
      481 ctcttcctca atctccaaaa acccgtcaaa actacttttt accccatcca aagtgtacgg
      541 cctgtgtccc cagaaatcgt taatgaaagc ctgctcgtcc cccctttcca aatactcgtt
      601 aatatcttta tatcgcaaat ctgccacaaa tgccttaccc ttaggaagaa cattcacgac
      661 ttcctctgtg gcctttctgc cagcttcatc attgtccatg agaacaataa tcttgctaaa
      721 ctgatctacc cattgatatt gctgccttac ttgtttagca gtgcccccct ccccaatagt
      781 ggagctcacc acagcaatct cgtcatactt cttggcattg ccactacgct gatgcatgtc
      841 atataacatt tggtaggcag acatagcatc gatctcccct gcggtgatca ctaagatacc
      901 tctgtgagtg cgaaacttcc actgcatgaa gaggtcacaa tcagaccctg cagaccctac
      961 gctataaaac ttctttgggt gttcccttat ttttagcccg caaaggctat ctttaatctc
     1021 tttctctaaa caccctttag tgacagggta aaatgtttta gccacctccc ctgtttccgt
     1081 gtcatactca tatctcacac ctaaagtagc agagatgtct gtacgaatgc ccctatagtt
     1141 tttactatcc actccagagt tttctttaat ctggttgtac aactcttcgt tccactctgt
     1201 tgccatatat tcatactcct gtggctctaa ttcttccaat tcctctaaaa aatcatcaga
     1261 tggttttgtc catccgcatg aaaaacaatg gtagccctca ccttccccgt agtagaaaag
     1321 gttatcccct gcttcatctc taccagattg acgacactta tagcaagggt ttttaccctc
     1381 cgccaaagag gcctcccaat ctttaaaatt catcacaaga gcctcccaca cattgtgtcc
     1441 tgcataacga cctccttgtt tatacttcac ctcctagctg cgaaacacac tataccacaa
     1501 ttactcttgt ttgtctatat cccagttttc aagcaacact ttcaccttaa agtttaatgg
     1561 catatccccc aaagcttctg ggaacgtttt gccctctttc cttgcattaa atagtacaga
     1621 agacatgggg ctgactttta cagacaaagc aaactctttc tggctttcaa catctttata
     1681 cttttcatac aaagactgtg catctttata taaccaaatc caagcattcg ccgccttatc
     1741 tataatttcc ctatcttctg ggaaataact aaggtactct tgatcttctc ttagcaccac
     1801 taactctgca acactccttg ggctattttt tatcccattc cccctaatac gatgtaaagc
     1861 cacataggca gggtttttaa ccttcaccac aggctgcatg agggaattat acaccacata
     1921 gccctcttcc atagggggca gacttttagc agcctctagc actccctcag ggcttcctag
     1981 tgggtatctc ttagcaatct tcactccctc aatccccttc tcttcattaa tatctgcata
     2041 ctctccactc tgagtgtgtc tcttgccaat aagagtgata gaggtgcttt catatggggt
     2101 taccacccta ttgtgagggg atgttagctc aaacatgtat gtaaaatctt tatccaactc
     2161 cccgcaggcc tcttgcatct caacaggaga tcccacaccc aaggctttct caaacagccc
     2221 cacaaaggta atacctgaag tccccactcc attttctcca aatgctgtgc ctctagtccc
     2281 tacatgccag tctccttgcc aataatagag aatcataaga gtcccatctg gcttttcata
     2341 cacataagaa gactcccaat caatatctgg ttgtgagcac tctgcctccc ctaagttgaa
     2401 gaatctatcg aaaggtcgcc tgatgatgtt tagatcttca tccaacgcca ctcctctaca
     2461 ctccataacc acaggatgtg ttttagggct attgatttgg taatagttaa acacactaag
     2521 tcctcctgcg tattctcgaa tcttgatgcc caagtcttcc ttcaaagaat ccaagccatt
     2581 acttttaata tactctatta agtgcatccc tctctcctta aaatcctcaa gatgacccca
     2641 ctatacccca acattttgaa gttgtcaaac agaatcttaa aagacctatt ttgagcccta
     2701 aaagacccaa tttgtagccc tcctttttag agtggtgttt ctcactctgt gtacacctaa
     2761 atccaagtgt ccattatttg gacgattaac acacttattc ctttagaatc atagacttac
     2821 tttcaaaact gtaggaaagg ggaggggagg gacgatactc cccttcatat gtacatatat
     2881 ttctactctt atttacatct gtatatatgt ataaatttgt atagaagaag agataaaatt
     2941 acatacaata aatacataat aaaagataaa tataatatct gttatagggg acatctgttc
     3001 ctatgtccac agatagtatc ccacacttga caaacagatt ataatacatt acaatacaaa
     3061 caatatatat atatatttta agatttactt tgtaagggag ggagaggtgt caataggtaa
     3121 gagtattatg ccagatattt ccacagtgga agtagaggaa aaatacttta agagaatctt
     3181 aaaggatcac ctactgctag agatgttaat atctggtgga gtggagaggt ggacatattg
     3241 ggatagtgcc ttagatactt tcaacaggct gaaagagtct tacgtgtcca cagctatgaa
     3301 agacaatgtt gtagaaaaga gagtttataa aaggaagtga gtgtatactc ctataaagac
     3361 tttagagacg ttctaagagg ttttaattcc ttccatatgg gtagggtagg gtggttagta
     3421 gaaaacctct ctgtgaggct ctcagggact cctaggggta ttactatctt tcttgttgtg
     3481 tactatttca atatctaggg gatattatgt gtaaagatga aaacaccacg tcaagaggtg
     3541 tatttgagaa aatgcttgag ggggagggag aaaatctcac ctttgagcag aaacaaaaaa
     3601 tattttataa gaagattagc acttctatag gggaggttgt agaagccaca gaaatgggag
     3661 gggtgtttgg caagatgttt gacagcgagg aatatgatga ttacactttc tcccagaaag
     3721 aggaactctt ttgtaagaga gtgggaatcc tagcagaaaa catagctaaa acttatatgg
     3781 agaatttaga gatagagttt attcctgaag aggacaacac ctactcttgt atcttaaaaa
     3841 tagattgtaa tcattttaaa gatttaatca acaaaggttt agaagctatg ttacaaggag
     3901 atgacctaga agagctggga aagataaaag aatagtaata ctctggaatt ggggtggata
     3961 cattttgtat aatgcctcta ggaatctgtg agaagctcat ggtgaggttt aaatggagca
     4021 acctatatgg tagcctacct attgctataa agctctttaa aagagctcta agagcttcct
     4081 gagacttaag acatatttac aagaggtggt gatgaagcat gtttttgtat ttgggagtaa
     4141 tctggctggg aggcatggca agggagcagc cttatttgcc agaaaacatt atggggctat
     4201 ttatggccaa ggtgtgggaa tgcaaggcca cagttatgct atacccacta aagattacaa
     4261 tttgagaaca ctccctttgg ccactataga aggttatgta gaagagttta aaagttttac
     4321 cagagaacac ccagacaaat tgtttattat aacccccata ggcacaggcc tagcaggcta
     4381 cagtaagaag gatatttggc ctatgtttaa aggtgtgggc agtaattgcg tgttttccca
     4441 cacttggtgt tgatcttgtt aaaggagagt gaaatgggaa gaaagttagt atccataagg
     4501 actatagatg aggtgaagcc cattgaaggg gcagacagga ttgaagcctg ccgtgtgggt
     4561 ggctggtggg tagtgggaaa gaagggggag tataaagtgg gggacaaagc catctattgt
     4621 gaaatagata gttggattcc tcacgagctt gctcctttcc tttctaagaa cagagagcct
     4681 agagagtatg gaggtattag aggggaaaga cttagtactg taaaactaag aggacaaatc
     4741 tctcaaggct tgttgttgcc cttgtctgtc ctaagggact atgctgttcc cgattgggcg
     4801 gaaggtaggg atttgtcggc atttttggga gtgatcaaat gggaaccccc tatccctgct
     4861 gtgttatctg gggaagcaag aggagttttt ccaaataata ttagtcgtac acaccaagag
     4921 agggttcaaa acttggggga tggtgaccat gttggatggc atctagtgac agaaaagcta
     4981 gatggggctt ccatgactgc ctatctccat gatggggaat ttggggtgtg ttctaggaat
     5041 gttaatctta gagaagatag ctccaacact tattggaaag tagcaagaga cttgtacttg
     5101 gaaaatgtgt tgaacagatt gtgtgaaaca actttctcgt ccgaagggta tgcaatacaa
     5161 ggtgagcttg ttggggaggg tatacaagga aacccatacg ggataaaggg gcatgacttc
     5221 tacgtgtttg acatctggga tataaagaag caagcttata taccttttaa tgttgttatg
     5281 tttttattac aaggaactgg attaaaaaat gtgcctgtgg tgggtaaaag attcttagat
     5341 agggtaaact cccaagagat tcttaaagat gtagatggcc tgaagtctgt tgtaaaccca
     5401 aaagtgttgg ctgagggagt tgtttataaa agcttagagc gtgggaacaa atctttcaag
     5461 gccatctcta acgaatggtt actgagagga aggagatgat tattaaatgg aaattaaggt
     5521 tgtgggtggc caacagctta tctaaatggc ttcataggaa ggatatgtat gggaagtttc
     5581 cttattatga tcaccatgtg tgagaaaagg ttggaatgtt tttaaacaag agggagtaaa
     5641 cttctcttga tttatagaca gaaaaagccc ccaagaggaa atcctcaagg gggcaaagtt
     5701 tttattgtta tatggcaata tgcttattgc ttctttttaa ggggaatgct tcctaccttc
     5761 tatctctatt atattgcaag attaaatcta gtctttctat aatctctgtt tttacagatt
     5821 ggatctgctt atctgtttct tttctactta ggagcatgtc ttccttaatc tctctcattg
     5881 tgtctaaaaa ctcacttcta gagagtttat cgttatatag gaatgtaaca cgctcttcca
     5941 ttttgtttac tctgtctttc atgtctgtgt agagcataga cataagagca attaggacgc
     6001 tgacccctat ggttaccacc ttgccagaga tcttgtcaaa tttgttgctc agatcatgca
     6061 ccctagattt tgcagcagat gtggtggagc agttattctc cgacattttt tagttcccta
     6121 ttataaatct gaatagaatc taccactgcc ttatacttac ctatgcaagt aatgttggca
     6181 agataccctt tctgaagact cttcacactg tccccagcac ccactggaga acaagggtgg
     6241 tgtataaggc tgtcagggag gggggcaggg acttccacgt acctctgcgc aggcttcatt
     6301 gagcaacctg ctaatatcgt caggcagagg actatcaaca gcttcctcat tcttatacac
     6361 ctcctccact ctattgatgt ttttgttgtt agcagactct agattttcaa acttctctct
     6421 gcttttctct aactctgcat tctctatttg ccttagttgt tttatgttgt ctaaagtgtg
     6481 ctctaaagac actacagtgc cttccaacac catcacatca taagaaagag aatttattgt
     6541 tgccttctgt tttgataatg tggcatacat tacagacatg accaccacac acactaagat
     6601 aacacctatg aatattgtat ggcttttaag gaaagatagc atcactcact cccttctccc
     6661 atacgatact ttttgaaata gtccatgttc ttttccatga tttcgtagta ggttcctatg
     6721 gctgctgcat catctacatt ccccaagcac cactcccttt cttctcccct gcgcaacacc
     6781 aaacctctta gcttcttctt attggcatac acccacctgc taagttggtc acaagctgat
     6841 ccatgatccc ccgccaatat gtaacttctt agggtgctcc tttgaaagtt tccagtgcca
     6901 ctgttgtagg caaaagatat aagggctgct ttttgccaat cagacgcata gttgtttttc
     6961 gtcatctgat ttatttgttg ctcaaaagat tttaaagtgt tggcaagcat ctgtatgcac
     7021 tcatcttcag tgtaggtggc tcccatctgt ataggcttgc cataaaggtc tctgcctgtt
     7081 tgcccccaac aggcggtagg aatacctata gggtcgaggt acacagtgtg tagcccgtct
     7141 ttgtttgtca cggagccttc ataggggtat attagataac ccccagatag ggctgctgct
     7201 acagacactc cgagagcagc cagcctttta gtaagttttc tctttacgcc catctgctct
     7261 ctcctttaaa ccctctacga aaacacaacg tcccagtcat tagggctctc atctggcctt
     7321 tttccagtat tagttgtgga cgctctgtaa agagaccctt catgctgtat gaaggatttt
     7381 ccagctatgt actcagtgct ttcgtcccac tctgggatac ctttctggaa aatataagaa
     7441 atagccctat cttgcctatt ctgtacccaa tt
//
LOCUS       DATGSI010000617         1948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_465300, whole genome shotgun sequence.
ACCESSION   DATGSI010000617 DATGSI010000000
VERSION     DATGSI010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465300"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VK031_09490"
     CDS             <1..320
                     /locus_tag="VK031_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HLS54195.1"
                     /translation="LDLASLTKALKKIMEARNLDQEDISAGEIRREEYSIVECTETIM
                     EKIQIEKRFKFSSLLSPDTNKQEIISFFLSMLELIRLGYIEALQDKSGSDILILGLNM
                     EES"
     gene            324..866
                     /gene="scpB"
                     /locus_tag="VK031_09495"
     CDS             324..866
                     /gene="scpB"
                     /locus_tag="VK031_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HLS54196.1"
                     /translation="MDIREVKSIIEGLLFTWGEPLDIKEIAKVLDIKKDKLEDILKEM
                     IDDFDYNRRGLRIIKIEDKYQLSTRPEHYEYIKKLSYSGVNRNLSKAAMETLSIIAYK
                     QPITKVEIDHIRGVRSDRAISTLLERQKIEEVGRLDRPGKPIIYCTTDVFLRSFGLES
                     LDELPELKNVEATPDELNEK"
     gene            934..>1948
                     /locus_tag="VK031_09500"
     CDS             934..>1948
                     /locus_tag="VK031_09500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HLS54197.1"
                     /translation="MEKKIISILVFFLAIGLMIMPVQGEGLNLSGSSFVLMEEESGRI
                     LIDKDSQKRMPMASTTKIMTALIGLENGNLNDKVLIDGESATIEGSSIYLQEGELIKL
                     KDLIYGLMLRSGNDAATAIAKHIAGSEDEFVLMMNKRAKEIGTKNTEFANPHGLSQKN
                     HYSTAYDLALITREAMKNSDFRDIFNCKTYTAQRDKNSYFINKNKTLWEYEGGDGGKT
                     GYTMEAGRCLVSSAKRNGMRLIAVSLNARNWFNDNYQLLDYGFEKYSPYMVYNKNQLI
                     DKIKIPNGYEKLNIVTSKELFYPLKDDEKKEIKVKVSINEDLKAPIERGESVGNIETY
                     LKGV"
BASE COUNT          745 a          253 c          415 g          535 t
ORIGIN      
        1 ggttagattt agcttcttta accaaggcat taaagaaaat aatggaagct agaaatttag
       61 accaagaaga tattagcgcc ggtgaaataa gaagggaaga atattctatt gtggaatgta
      121 cagagaccat aatggagaaa attcaaatag aaaaaaggtt caaatttagt agcctactaa
      181 gccctgatac aaacaaacag gaaattatat ctttttttct atctatgttg gaactaatca
      241 gattaggcta tatagaagcc ctacaggata aaagcggttc tgatatactt atattaggac
      301 tgaatatgga ggagagctaa ggaatggaca taagagaggt taaatcaatt atagaaggct
      361 tattattcac ctggggagaa cctttggaca taaaggaaat agccaaggtg ttagatataa
      421 agaaggataa attagaggat attttgaaag aaatgataga tgattttgat tataataggc
      481 ggggtttaag aataataaaa attgaagata aataccagct cagtaccagg ccggaacatt
      541 acgagtacat aaaaaagctt agctactctg gagtaaatag aaacctatct aaagcggcca
      601 tggaaacctt gtctataatt gcttacaagc aacctataac caaggttgaa atagatcata
      661 ttagaggggt tagatcagat agggctataa gcaccctatt ggaaaggcaa aagatagagg
      721 aagtaggccg gttggacagg cctggtaaac ctataattta ctgtacaaca gatgtgtttt
      781 taaggtcttt tggactagaa tctttagatg aactacctga gttgaagaat gtggaagcta
      841 ctcctgatga acttaatgaa aaataataca tcttgtatta tttttcgtgt tttttggtaa
      901 catatttagt ataaactatc aggagtgata aagttggaaa aaaagataat atcaatcctt
      961 gtattttttc tggccattgg acttatgata atgccggtgc aaggagaggg tttaaattta
     1021 agtggatcta gctttgtact aatggaagaa gagtctggca ggattttaat agacaaagat
     1081 agtcagaaaa ggatgcctat ggccagtaca acaaaaataa tgacagcttt aataggctta
     1141 gaaaatggca atttaaatga caaggtttta atcgatggag aaagtgcaac tattgaaggc
     1201 tccagtatat atttgcaaga aggggaatta ataaagttaa aagatctcat atatggctta
     1261 atgcttaggt ctggcaatga tgcggcaaca gctattgcca aacatatagc tggatcagaa
     1321 gatgagtttg ttcttatgat gaataaaagg gccaaagaga ttggaacaaa gaatacagag
     1381 tttgctaacc cacacggatt aagccaaaaa aaccattatt ctactgccta tgatttagcc
     1441 ctgatcacta gggaggccat gaaaaatagc gatttcaggg atatatttaa ctgtaaaact
     1501 tatacagccc aaagggataa aaattcctac tttataaaca aaaataaaac cttatgggaa
     1561 tatgaaggtg gagatggagg taaaactgga tatactatgg aggctggcag atgcttggtt
     1621 tccagtgcca aaagaaatgg aatgagatta atagctgtaa gcctaaatgc caggaattgg
     1681 tttaatgata actaccagtt gcttgattat ggctttgaaa agtattcgcc ctatatggtt
     1741 tataataaaa atcaactgat agataaaata aaaattccaa atggttacga aaaactaaat
     1801 atagtaacct ctaaagaatt attctatcct ttaaaagatg atgaaaagaa agaaataaaa
     1861 gtaaaggtta gtataaatga agatttaaag gccccaattg agaggggaga gtctgtaggt
     1921 aatatagaaa cctaccttaa gggagttt
//
LOCUS       DATGSI010000618         1973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_418845, whole genome shotgun sequence.
ACCESSION   DATGSI010000618 DATGSI010000000
VERSION     DATGSI010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418845"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VK031_09505"
     CDS             complement(<1..290)
                     /locus_tag="VK031_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS54198.1"
                     /translation="MALIDIRNLTFEYIGGLEPIFEDLNLQLDTDWKLGLIGRNGYGK
                     TTFLQLLMGKYLYQGAIIKPIPMAYFPFSIDNYEMMTLDLLRSLQADFQQWK"
     gene            complement(667..1275)
                     /locus_tag="VK031_09510"
     CDS             complement(667..1275)
                     /locus_tag="VK031_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HLS54199.1"
                     /translation="MHVLDKSSWNVRTMTKVSVLGTISFILMFFKFPLAWLAPPFLKM
                     DISDVPSLIGAFALGPVAGVLIQLLKNILNLAFEGTSTATVGEFANFLSGGIFAFIAG
                     YIYQKDRNFKNAILGMGMGIIAMTAIICIANYYFIFPLYAKFLGIPMDTIISMGSAVT
                     KKVTDLRSLIVYTVVPFNLIKGALTAAITILIYKKVSPLLQK"
     regulatory      complement(1358..1472)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1575..>1973)
                     /locus_tag="VK031_09515"
     CDS             complement(1575..>1973)
                     /locus_tag="VK031_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLS54200.1"
                     /translation="VDDLAKEGKGAIVGPTLGDRSKFELKNMTFDTPKILNDGGVKIA
                     ITTDAPVVPLQYLSLCAGLAHKAGLDEMEAFKAITINPAEIIGLDDRVGSLEVGKDAD
                     IVIFDGNPIKDVDYHTFMTIIDGKVVYRRK"
BASE COUNT          639 a          378 c          277 g          679 t
ORIGIN      
        1 aatttccact gttgaaaatc cgcttgtaaa cttcttaata aatccaaggt catcatttca
       61 tagttatcga tggaaaaggg aaaataggcc ataggtattg gtttaatgat tgcaccttga
      121 tagagatatt tacccattaa aagttgtaaa aaggtggtct tcccatatcc attcctacct
      181 attaatccta atttccaatc tgtgtctaat tgtaaattta aatcttcaaa tataggttct
      241 aaacctccta tatattcaaa ggttaaattt ctaatatcta ttaatgccat ataagattct
      301 cccttcgtaa attaatagat agcgagaatc tccttgtgat cttttattta aaaacaaaaa
      361 ctgagagtag aaacctctca gtaaaagaca ccaaaaaatt taattatatt tggtaatagg
      421 ctcgattata tattgaaaga gatttctcgc taaaatgggc acaagaaaat aaaccatggg
      481 ttaaaattta tatcttgtac ttattaaatt ccttatctag caagaaatct gttcatggat
      541 acaccctctc tcattttctt acttaaatag taccatattt tttccttact ttctagtatt
      601 ccataaaaaa cagagagtaa tctactctct gttaaataaa attaaataca taattaatat
      661 aatcttttac ttctgtagta agggtgaaac ctttttatat attaatatgg taatggctgc
      721 tgttaaggct cccttaatta ggttaaatgg tactactgtg taaactatta agcttcttaa
      781 atcagtaact tttttagtaa cagccgaacc catactaata atagtatcca ttggaatgcc
      841 tagaaatttt gcataaaggg gaaatatgaa ataataattg gctatacaga ttatggcagt
      901 catagcaatt atccccatac ccattcctag aatggcattt ttaaaattcc tgtctttttg
      961 gtaaatatat cctgcaataa aagcaaatat acctcctgat agaaagttgg caaattcccc
     1021 tactgtagct gtgctagtcc cttcaaaggc tagatttaaa atatttttta aaagctggat
     1081 taagactccg gctacaggcc ctaatgcaaa agctcctatt aaggatggta catctgaaat
     1141 atccatcttt aaaaagggtg gtgccagcca ggccaatgga aatttaaaaa acattaaaat
     1201 aaatgaaatt gtacccaata ctgatacctt tgtcatagtt cttacattcc agctactttt
     1261 gtctaaaaca tgcatacata tccctccatt ttttatgctt cagggataaa aaaaccccga
     1321 agtattcttc ggggaattag acataaacaa ttgtctaata catcttcttt catccagact
     1381 ataactgtcg gccttggatt ttcaccaagt cagccttaag gctcgcgggc tttaccgccg
     1441 gtgaggaatt ccacctcccc ctgaagatat atcttttatt taattttatt atatttaatc
     1501 gtttataatt tgtcaagcac tttttgttac ttttttgata atctcagact attaaaattg
     1561 attatcctgc tctattattt tctcctatat acaacttttc cgtctataat agtcataaat
     1621 gtgtgataat ctacatcttt aataggattt ccatcaaaga taacaatatc tgcatctttt
     1681 cctacttcta ggctcccaac cctatcatct agccctataa tctctgctgg gttaatagta
     1741 atagccttaa aggcctccat ctcatctaaa ccagccttat gagccagtcc tgcacataag
     1801 gataggtatt gcaagggaac tacaggagca tctgtggtta tagcaatctt tactcctccg
     1861 tcattcaata tttttggagt atcaaaggtc atattcttta attcaaactt agatctgtct
     1921 cctaaggtag gtcctactat ggccccctta ccttctttgg ctaagtcatc tac
//
LOCUS       DATGSI010000619         1790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_418925, whole genome shotgun sequence.
ACCESSION   DATGSI010000619 DATGSI010000000
VERSION     DATGSI010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418925"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..739)
                     /locus_tag="VK031_09520"
     CDS             complement(11..739)
                     /locus_tag="VK031_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HLS54201.1"
                     /translation="MAYREAVILLNEKIGPNIYKLVVEGQFEGEPGQFYMLRGWNGLD
                     PFLARPFSIGDLDEGKISFFYEERGRATRIMSDLQKGQKLSLLGPLGRGFPYKRKGRI
                     ALVAGGIGIAPIYYLAKRLAVKGDLFLGFRNHTYLTEELTPYGEEIFISTEDGSIGHK
                     GYIVDILDPKKYDLIMACGPSPMLKAIMDKSGDKIKLYLSMESHMGCGVGTCLGCTIK
                     TSSGMERVCKEGPVFSGREVLFYD"
     gene            complement(756..1610)
                     /gene="pyrF"
                     /locus_tag="VK031_09525"
     CDS             complement(756..1610)
                     /gene="pyrF"
                     /locus_tag="VK031_09525"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HLS54202.1"
                     /translation="MIIDRLYDEVEKKGPVCVGLDSRLAHIPSWIKEKYQDLDEIIFQ
                     FNKKIIDATEDLVAIYKPQIAFYEAYGIKGLLAYSRTLEYIRKKQLLSIGDIKRGDIS
                     STAVMYGRAHFQGDFEADFITLNPYMGFDSITPYLKYLDTGKKGIFVLLRTSNPGAKD
                     IEYLKANNKRIYYHVGDKLEEIGRSYIGSRGYSLIGAVVGGTYREEAEEIRERYKNMF
                     FLIPGYGAQGASGGDVNLYLEKGNGGVVNSSRGIIRSYKNYGDGEKNFQKYTRQAVEE
                     MIKDIRQD"
BASE COUNT          502 a          447 c          244 g          597 t
ORIGIN      
        1 aatttccacc ttagtcatag aagagaacct cccttcctga aaatactggt ccttccttgc
       61 agaccctttc catgccagaa ctggtcttta tggtgcaacc taaacaggta cctacaccac
      121 atcccatatg actttccatg gacaggtata gcttaatttt atctccactt ttatccataa
      181 tagcctttaa cataggacta ggtccacaag ccatgattag atcatacttt tttggatcaa
      241 gaatatctac aatataaccc ttatgtccta tggaaccatc ttcagtggat ataaaaatct
      301 cttctccata aggagtcaac tcttccgtaa gataggtgtg atttctaaag cctagaaaca
      361 aatctccctt tacagctaac cttttggcca aataatatat tggagctata cctatgccac
      421 cagccaccaa agcaatcctt cccttccttt tgtaaggaaa tcctctccct aaagggccta
      481 acaaactaag tttttgcccc ttttgtaaat ctgacattat cctggtagct ctgcctcttt
      541 cctcataaaa aaaactaatc tttccctcat ctagatcccc tatgctgaag ggcctagcta
      601 aaaagggatc cagcccattc caacccctta gcatataaaa ttgacctggt tcaccttcaa
      661 actggccttc caccactaat ttatatatgt tgggacctat cttttcattt aataaaatta
      721 cagcttccct ataagccata aggcacctcc tccccttaat cttgcctaat atctttaatc
      781 atctcctcta cagcctgcct agtatatttt tgaaaatttt tctccccatc cccatagttt
      841 ttataagacc tgattattcc gcgagaggaa ttaaccactc caccattgcc tttttctaga
      901 taaaggttta catcccctcc actagctcct tgggctccat agcctggaat aaggaaaaac
      961 atatttttgt acctttctct gatctcttcc gcttcctccc tataggttcc acctactact
     1021 gcccctatta atgaataacc cctgctacct atatatgatc tgcctatctc ctctaatttg
     1081 tcacctacat ggtagtagat ccgcttatta ttggccttta aatattcaat atctttggcc
     1141 cctggatttg aggttcttaa aaggacaaag attccttttt ttcctgtatc caagtacttt
     1201 aaataggggg ttatactatc aaatcccata taaggattaa gggttataaa gtcagcttca
     1261 aaatctcctt gaaaatgtgc cctcccatac atgactgccg tggaagatat gtctcccctt
     1321 ttaatgtcac ctatggacag aagttgtttt tttcttatat attctaatgt cctactatag
     1381 gctagtaggc ctttgatccc atatgcctca taaaaggcta tctgtggttt ataaatggcc
     1441 accaaatctt ccgtagcatc tataattttt ttgttgaatt gaaatattat ttcatctagg
     1501 tcctggtatt tttccttgat ccaactggga atatgggcaa gtcttgagtc taaacctaca
     1561 caaactggtc cctttttttc tacttcatca taaagcctgt ctataatcat agctaatcca
     1621 cccctttaaa agatttacta aagtatttta tttgtccacc ctttactgtc aacttaacct
     1681 tgccataaaa acttttccct tcaaagggtg tattcctgga tttagatgca aactggctag
     1741 aggctatttg aaatctttca tctaaatcaa gtagcaccac atcaccatca
//
LOCUS       DATGSI010000620         1129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_371314, whole genome shotgun sequence.
ACCESSION   DATGSI010000620 DATGSI010000000
VERSION     DATGSI010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371314"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VK031_09530"
     CDS             <1..846
                     /locus_tag="VK031_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HLS54203.1"
                     /translation="WRKYTMNDINSWCVVSVPTKSWAKRVFPNLNEDEAVDQLWQAIF
                     KATRMDLKDPVAAWQKHIAGLEKKVEYLNRKSFKTLYYSSSNGTKLKVDLPKDHIWAG
                     GGGYNAKGTYFVANMPTEEVFTMPLKTGVNGVVYSTKPLNYGGKLVDNFKLTFKDGKV
                     VDYEAQIGQEVLRDLFAIDEGAKYLGEVALVPYDSPISKSNIVFMNTLFDENASCHFA
                     FGKAYPTNLKDGEQMTDEELEAHGVNNSLTHVDFMIGTKDLSIIGEDYSGEKIQIFKD
                     GNWAI"
     gene            complement(923..>1129)
                     /locus_tag="VK031_09535"
     CDS             complement(923..>1129)
                     /locus_tag="VK031_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54204.1"
                     /translation="ELQKMGQTLPSEMSFKTTEIVMGDTSARRKDIKKKEIAKDSQTT
                     TSVEMKDKSTGDKEKKGGFGFLFK"
BASE COUNT          342 a          202 c          230 g          355 t
ORIGIN      
        1 tggagaaaat ataccatgaa tgatataaat tcctggtgtg tggtgtctgt tcctaccaaa
       61 tcctgggcta aaagggtatt tccaaattta aatgaagatg aagctgtaga tcagctttgg
      121 caggccatct ttaaggctac cagaatggac cttaaggatc ctgtggctgc ttggcaaaag
      181 catatagcag gtctagaaaa aaaggtagaa tatttaaata ggaaatcctt taagaccctt
      241 tattactcat cctcaaatgg aacaaagctt aaggtggacc tacctaagga ccatatttgg
      301 gctggaggtg gaggatataa tgctaaggga acttattttg tagccaatat gcctacagaa
      361 gaagtattta ccatgcctct aaagactggg gtcaatgggg ttgtatatag tactaagcca
      421 ttaaattatg gaggtaagtt ggttgataat tttaaactta cctttaaaga tggaaaggta
      481 gtagactatg aggcccaaat aggccaagaa gttcttaggg atctatttgc tatagatgaa
      541 ggtgccaagt atctagggga agttgctttg gttccctatg actcccctat ttctaaatca
      601 aacattgtct ttatgaatac cttgtttgat gaaaatgcat cttgccattt tgcctttggt
      661 aaggcctatc ctactaattt aaaggatgga gagcagatga cggatgagga attggaagcc
      721 catggcgtta ataactcgct aacccatgtg gattttatga taggaaccaa ggacctatca
      781 ataatcggtg aagactatag tggagaaaaa atccaaatat ttaaggatgg caattgggcc
      841 atataaaaaa gtccaagtga tttccacttg gactttttat ttggctaata ggatccaact
      901 aacctatgga taaccattat cattatttaa acaggaatcc gaatccacct ttcttctcct
      961 tgtcacctgt acttttatct ttcatttcca cactggtggt ggtttgacta tctttggcaa
     1021 tctccttctt ttttatatct tttctcctag ctgaagtatc tcccataact atttcagtag
     1081 tcttaaagct catctcacta ggcaaagtct gtcccatttt ctgtagttc
//
LOCUS       DATGSI010000621         1059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_610180, whole genome shotgun sequence.
ACCESSION   DATGSI010000621 DATGSI010000000
VERSION     DATGSI010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610180"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VK031_09540"
     CDS             complement(<1..691)
                     /locus_tag="VK031_09540"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HLS54205.1"
                     /translation="MGIPIKIFTSFYWKTYKMRDLLVRSLSGLFYVSLILSTLFIDRN
                     WTIILFGILGFLTLRELLNLLKTKAYLSYLLLFFSFIFLYIEKDTIFVPVLLIASCIF
                     NAVLAYQLFASIPLKNNSLYRYGLSFLYLTGGFILITKIADANLVNSSSKLLVSIFLL
                     IWASDSFAYLIGVNFGKRKLFPSVSPNKSVEGLIGGLVGAILVSIIVAFKIYPSIAIE
                     NWIIIAILTSAL"
     gene            complement(642..>1059)
                     /locus_tag="VK031_09545"
     CDS             complement(642..>1059)
                     /locus_tag="VK031_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HLS54206.1"
                     /translation="SILLENDWYETNVCCLNSELAKRFDGYNLKFMSKNNAASFMLST
                     CESLVADNGSILLSSNQLKGNKLWQLPDNIVIFATTSQIVSNISDGMRIIKMQNKNNI
                     PVNISAIRHFGSMQKEDFMTYGNSNKNLYLILLEDL"
BASE COUNT          363 a          170 c          185 g          341 t
ORIGIN      
        1 ctaaggcact agttaaaatt gctatgatta tccaattttc tatagcgata cttggatata
       61 ttttaaaagc tacaataata cttacaagta tagctcccac taagccacca ataaggcctt
      121 caacgctttt atttggcgat acgcttggga agagctttct tttcccaaaa ttcactccga
      181 ttaggtatgc aaaactatcg ctagcccata ttaatagaaa tatggaaact agtagttttg
      241 aagagctatt tactaaatta gcatcagcta ttttggttat aagtataaag ccaccagtca
      301 gatataagaa actaagaccg tacctataaa gtgagttgtt ttttaatggt atagaagcaa
      361 ataactgata tgctaataca gcattaaaaa tacaactggc aattaataat acaggcacaa
      421 atatagtgtc cttttcaata taaaggaaaa taaagctaaa aaatagtagg aggtaagaaa
      481 ggtaggcttt ggttttaagt aggtttaaaa gttctctaag ggttaaaaaa cctaagatcc
      541 caaatagtat tatggtccaa tttctatcta tgaataaagt tgataggata agggatacat
      601 aaaacaaccc tgaaagggaa cgaacaagta gatctctcat cttataagtc ttccaataaa
      661 atgaggtaaa gatttttatt ggaattccca taggtcataa agtcttcctt ctgcatgctt
      721 ccaaaatggc gtatggcgct tatgtttaca gggatattat ttttgttttg catctttatt
      781 attcgcatac catcacttat attactaaca atttgactag tggtcgcgaa tataactatg
      841 ttatctggta gttgccatag tttgtttcct tttagttgat tggatgagag taggatggag
      901 ccattatcgg cgacaagaga ctcgcaagtt gacagcataa atgaggcagc gttattttta
      961 ctcataaact ttaagttgta accatcgaat cttttggcca gttcagagtt taaacaacat
     1021 acgttcgttt cataccaatc gttttctaat aagatgcta
//
LOCUS       DATGSI010000622         1137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_562731, whole genome shotgun sequence.
ACCESSION   DATGSI010000622 DATGSI010000000
VERSION     DATGSI010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562731"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VK031_09550"
     CDS             <1..363
                     /locus_tag="VK031_09550"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54207.1"
                     /translation="GTYSLHTASKEVELNVEVDREVIQADGADLSYIRVGLVDEKGNH
                     NLQLVKNISVNVEGEGYLQGFGSADPLTENHYYNTSWDTYDGYVLAVIRSTNNAGEIR
                     VTFTAEGCEKKEVIIKTK"
     gene            512..895
                     /locus_tag="VK031_09555"
     CDS             512..895
                     /locus_tag="VK031_09555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HLS54208.1"
                     /translation="MKDEIVELYFSIEAEYRKDAVFIMNDETAFYLRTLKDDDGNYLW
                     NSNNDTIFGKEVITSPYMPSIESGKQPIIFGDLSFYWVIERKPLAIQVLNELYSNQGQ
                     IGFIGFERVDGKLIRPEAVKTIKMA"
BASE COUNT          418 a          153 c          236 g          330 t
ORIGIN      
        1 ggcacatatt ctcttcatac tgcgtcaaaa gaagtggaat taaatgtaga ggttgatcgg
       61 gaagtaattc aagcagatgg agcagatttg tcctatattc gtgtaggtct agtagatgaa
      121 aagggcaacc ataacttaca acttgttaaa aatataagtg ttaacgtgga aggggaaggt
      181 tatctacaag gctttggaag cgctgatcca ttaacagaaa atcattatta taatacaagt
      241 tgggatactt atgatggata tgtacttgct gtaattcgtt ctactaataa tgctggagaa
      301 attagagtta catttactgc agaaggttgt gaaaagaaag aggtaataat taaaacaaaa
      361 taaaactccc aagtgtattg ttggagcacc ggaacctgac ataacaatta ataaagcttt
      421 tctagcagga gcaaaagtca ccgtttttgg ttgttatggc tggttcctac aggcaaaata
      481 gagttaagta gtttctttat aaattaaaac aattaaagat gagatagtag aactgtattt
      541 ttcaatagaa gcagaataca gaaaagatgc tgtatttatt atgaatgatg aaacagcttt
      601 ttatcttaga acattaaaag atgatgatgg taactattta tggaactcta ataatgatac
      661 gatctttggt aaagaagtta ttacatctcc ctatatgcca agtattgaat ctggcaaaca
      721 gccaattatc tttggagatc tatccttcta ctgggtaatt gaaagaaagc ctttagcaat
      781 acaggtatta aatgaactat acagtaatca aggtcagata ggcttcatag gctttgagag
      841 ggtagacggt aagcttataa gaccagaggc agttaaaaca attaagatgg cttaaaaaca
      901 tacggaccct ttagctcaaa aatgagcaat agctaaagat gtatatgtat aaattatttc
      961 taggcactga acccaaatct gggctcagtg tctaactctt taaaaggaag gagtgatttt
     1021 gtgattaaag gcgagaaatt taggactagc aaaaaagaaa tagctggtac tacctatata
     1081 gttgaatctg gggaatgtga agaggctaaa gaaacacctt attctaaaat tgaaagg
//
LOCUS       DATGSI010000623         4016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_89225, whole genome shotgun sequence.
ACCESSION   DATGSI010000623 DATGSI010000000
VERSION     DATGSI010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89225"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VK031_09560"
     CDS             <1..214
                     /locus_tag="VK031_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54209.1"
                     /translation="LSNLLLNFLTKPDFIAYEHKYANNLSFTICRWIYKTPAIAYTIQ
                     SEDDLVKNKDRFDLFIFDSFIPKSRP"
     gene            complement(261..563)
                     /locus_tag="VK031_09565"
     CDS             complement(261..563)
                     /locus_tag="VK031_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HLS54210.1"
                     /translation="MMYNSKIRSKQTDELFEAILKLETLEECYRFFEDICTIKEIQAI
                     SQRLEVAKLLTANKTYNEIEEETGASTATISRINRALNYGVDGYNLVLRKLGIIDE"
     gene            complement(695..2365)
                     /locus_tag="VK031_09570"
     CDS             complement(695..2365)
                     /locus_tag="VK031_09570"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HLS54211.1"
                     /translation="MQTDVQIAQGAKMLPISKVAEKLGIPEDKLIQYGNYKAKISLDL
                     MEDLKNREDGKLVLVTAISPTPAGEGKTTTNIGLSMGLNKIGKKAITTIREPSLGPSF
                     GIKGGAAGGGYAQVVPMEDINLHFTGDFHAITTAHNLIAALIDNHIHQGNQLGIDPRR
                     VVWKRVLDMNDRALRNIVVGLGGRSNGLPREDGFDITVASEIMAIFCLARDLEDLKER
                     VGEIVLAYKFDGSPVLAKDLNAQGAVALIMKDAMNPNLVQTLENTPALIHGGPFANIA
                     HGCNSLVATKMALKLADYTITEAGFGADLGAEKFFNIKARLGNLKPDAAVIVATVRAL
                     KHHGGAKKEELEIENLDQLSKGMANLEKHIENVKKFGVPVLVAINKFPTDTEGEINYV
                     INRCQELGVDAVLSEVWSKGGAGGEELAKAVVRACENYQSNFQPIYQVEDCIKTKIEK
                     IAREIYGADGVDFTGKAEKEIENLESLGLDKMPICMAKTQYSLSDNPALLARPEGFRI
                     TVRDIRISKGAGFLVALTGDIMTMPGLPKVPAANKIDILPTGEIVGLF"
     gene            complement(2492..3742)
                     /gene="purD"
                     /locus_tag="VK031_09575"
     CDS             complement(2492..3742)
                     /gene="purD"
                     /locus_tag="VK031_09575"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HLS54212.1"
                     /translation="MKVLVIGSGGREHTLCWKLGQSSKVKKVYCAPGNGGTGEVGENI
                     EIAANDIDSLLDFAIKKGIDLTVVGPEGPLVLGIVDRFKEKGLKIFGANKAAARLEGS
                     KEFSKTFMEKYEIPTARYKSFSNYDEAVKGLKGFDYPLVIKADGLCFGKGVVICNTKE
                     EAIQCLEEILVDRVFGEEGSTVIIEEYLDGEEVSLLCLVTGDNIIPMEPARDYKRIFD
                     QDQGPNTGGVWCFSSSKLLPEKLRNTINKNILERIRLGLKEEGLGYKGILFIGLMIKE
                     NDPRVLEFNVRFGDPETQVLLPRLESDLGKLLLKSIDGGLKEEDLCWSQKECVTVIAT
                     SKGYPGDYEAGFPISGIERMDETIIPFHNGSRNEGGKLLTNGGRVLSITALGNTVEEA
                     RNIIYNNIKKISFEGMHYRKDIGK"
     gene            complement(3758..>4016)
                     /gene="purH"
                     /locus_tag="VK031_09580"
     CDS             complement(3758..>4016)
                     /gene="purH"
                     /locus_tag="VK031_09580"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034726.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HLS54213.1"
                     /translation="GIGLGEVNRFWAVENAIGRAGNKVKGSVLASDGFFPFKDSVEAL
                     GKAGVSAIIQPGGSIRDKEVIKEANKHKIAMVFTGNRHFKH"
BASE COUNT         1052 a          962 c          563 g         1439 t
ORIGIN      
        1 tttaagcaat cttttactta actttttaac aaaacctgac ttcattgcct atgagcataa
       61 atacgccaat aacctgtcat tcaccatatg taggtggata tataaaactc ctgccatagc
      121 ctatactatt cagtcagagg atgacctggt taaaaataaa gataggtttg atctatttat
      181 atttgatagt tttattccta agtctaggcc ttagttacaa atgaaaagga tttccctatg
      241 ggggatcctt tctacagatc ttattcgtct atgattccta attttctaag aaccaagtta
      301 tacccatcta ctccataatt tagtgctcga ttaattctac ttatggtagc tgtgcttgct
      361 cctgtttctt cttcaatctc attataggtt ttatttgctg ttaataattt ggcaacctcc
      421 aacctttgtg atatggcttg tatttctttt attgtacata tgtcttcgaa aaatctataa
      481 cattcttcca gagtttccaa ctttaaaatt gcttcaaaga gttcatcagt ttgtttgctc
      541 ctaattttag aattgtacat catattagcc cactcctttt tttcaatagt acattcatag
      601 tttaacacat tcaagtgttt atgtaaaact aaattgtttt tatattttta ttcaattgga
      661 caaaagaaaa agatttgtgt ctgacacaaa tcttttaaaa gagacctact atttcacccg
      721 ttggtaatat atctatttta ttagcagcag gtacctttgg taaacctggc atggtcatta
      781 tgtctcctgt taaggccacc aaaaatcctg caccctttga aatccttata tcccttactg
      841 ttattctaaa accttcaggc ctggccaata gggctggatt atcagataaa gaatattgag
      901 tcttggccat gcaaataggc atcttatcaa gccccaggct ttctaagttt tctatttctt
      961 tttcagcctt acctgtaaag tcaaccccat ctgctccata tatttccctt gctatttttt
     1021 ctattttagt ttttatgcag tcctccacct ggtaaatagg ttgaaaatta gattggtaat
     1081 tttcacatgc cctaactacc gccttagcca gttcttctcc accggctccg cctttggacc
     1141 atacctctga caatactgca tctacaccta attcctggca cctatttatt acataattaa
     1201 tttccccttc cgtatctgtt ggaaacttat ttatagccac caggacaggg actccaaatt
     1261 ttttcacatt ctctatatgt ttctctaaat ttgccatccc cttacttagt tggtctaaat
     1321 tttctatttc tagttcttcc ttttttgccc ctccatgatg ttttaaggcc ctaactgtcg
     1381 caactataac agctgcatct ggctttaaat tgcccaagcg ggcctttatg ttgaagaatt
     1441 tttccgcccc taaatctgcc ccaaaacctg cctctgttat ggtgtaatct gccagtttta
     1501 gggccatttt tgtggcaact aaagaattac aaccatgggc tatattagca aagggccctc
     1561 catgaattaa ggctggagta ttttccaggg tttgaaccaa attaggattc attgcatcct
     1621 tcataatcag ggctacagct ccctgggcat tcaagtcttt ggccaaaact gggctcccat
     1681 caaacttgta ggctaataca atctctccta ccctttcctt taagtcttct aaatccctag
     1741 ccaaacagaa gatggccata atttctgaag ccactgtaat atcaaatcca tcttccctag
     1801 ggagcccatt actcctacca cctaaaccta ccacaatatt tcttaaggcc ctgtcattca
     1861 tatctagcac ccttttccaa accactcttc taggatctat acctagttgg tttccctggt
     1921 ggatatggtt atctataagg gctgctatca gattatgggc tgttgtaatt gcatggaaat
     1981 ctcctgtgaa atgaagatta atatcctcca tagggactac ttgagcatat cctcccccgg
     2041 ctgctccgcc tttaattcca aaacttggcc ctagggaagg ttcccttatg gtggttattg
     2101 ccttttttcc tattttattt aaccccatgg aaaggcctat atttgtagtt gtttttccct
     2161 ctccagctgg agtagggctg atggctgtca ccagtactag cttcccatct tccctatttt
     2221 ttagatcttc cattaaatct aaagaaatct ttgccttata atttccatac tggattagct
     2281 tatcttctgg aatacccagt ttttcagcca ccttactaat aggtaacatt ttagcccctt
     2341 gggcaatttg gacatcagtt tgcacagtat ttcctcctta tgtaaaattt cctcaatagg
     2401 cttaggccca tcacatttaa ttaggactat tatctactgt tataaatatt tttaccctta
     2461 taaaacccta gtattcaatt ctttacacaa tctatttgcc aatatccttc ctataatgca
     2521 taccttcaaa gcttattttt ttaatattat tatatatgat atttctggcc tcttctacag
     2581 tatttcctaa ggctgtaatg cttagcaccc tgccaccgtt agttaatagt tttcctcctt
     2641 catttctgct tccattatga aatggtatta ttgtttcatc catcctttct attccagata
     2701 taggaaatcc tgcttcataa tcaccagggt agccctttga tgtggctatt acagtgacac
     2761 attctttttg tgaccagcaa aggtcttctt ctttcaagcc tccatctatg gattttaaaa
     2821 gtaacttccc taaatcactt tccagcctag gtagtaaaac ttgggtttct ggatctccaa
     2881 atctaacatt aaattctaaa accctaggat cattttcctt tatcatcagg cctataaaaa
     2941 ggattccctt atagcctagc ccttcctctt tcaagcctag ccttattctt tctagtatgt
     3001 ttttgtttat tgtatttctt aacttttctg gaagtagctt gctggaggaa aagcaccata
     3061 caccacctgt attagggccc tgatcttgat caaatattct tttatagtct ctggccggtt
     3121 ccatagggat tatattatct cctgttacca aacataaaag tgaaacctct tctccatcta
     3181 agtattcttc tataattaca gtgcttcctt cctctccaaa gactctatct accaatattt
     3241 cctccaaaca ctgtattgcc tcttctttag tattacagat aactactccc ttaccaaagc
     3301 ataggccatc tgcctttatt accaagggat agtcaaatcc cttcaaacct ttaaccgcct
     3361 catcataatt agaaaaactt ttgtacctgg ctgttggtat ttcatatttc tccataaagg
     3421 ttttagaaaa ctctttgcta ccttctagtc tagcagccgc tttattggct ccaaatattt
     3481 tcaggccttt ttccttaaat ctatcaacta ttcccaacac caaaggccct tccggtccaa
     3541 ctacagttaa atctatacct ttctttatgg caaagtccaa taaactatca atatcattgg
     3601 ctgctatctc tatgttttct cctacttctc ctgttccccc attacctgga gcgcagtata
     3661 cttttttcac cttgctggat tgtcctaatt tccagcataa ggtatgctcc ctacctccac
     3721 tacctataac taaaaccttc ataagtccct cctagtttta atgtttaaag tgcctattac
     3781 ctgtaaatac catggctatc ttgtgtttat ttgcctcctt aattacttcc ttatccctga
     3841 tggagccgcc tggttgtata atggcgctaa ctcctgcttt acctaaagct tctactgaat
     3901 ccttaaaggg aaagaagcca tctgaagcca acacacttcc ttttactttg tttccagccc
     3961 ggcctatagc attttccacc gcccaaaacc tatttacctc tccaagtcca atccca
//
LOCUS       DATGSI010000624         1728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_465768, whole genome shotgun sequence.
ACCESSION   DATGSI010000624 DATGSI010000000
VERSION     DATGSI010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465768"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1728
                     /locus_tag="VK031_09585"
     CDS             <1..>1728
                     /locus_tag="VK031_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLS54214.1"
                     /translation="LDHDLTVEEIFDTLIKNYEVDENLSKYEARGIFGLYNQLKKQGY
                     LAYQPINIAYGIKETTVAKIEEGLMEFSGIDVSIEPVRYYPEGTTAAHILGYLGKISQ
                     PNEIQKYIGEKNYSPGTIIGKTGIEESFEDQLSGEHGIKRIEVDSVGNTTSVISEIKP
                     VPGDNLYLSIDLDLQKKAEEVLEHSLTEIQKGGTYKSPWGDYKYSINRSKRRPYKYAT
                     SGAVVAVDVKTGQVLASASFPAYDPNLFATGITDSDWLSLFPENEDDLLAPRPLYNIA
                     TQTALEPGSIFKMATALTALEKGLSPDKKIRDMGYVNVGKQMNCLLWTMSRRTHGNVN
                     VYEALRDSCNYYFYSLALGQNQRTGESIGTKIEIEDIVDMVRKLGMDDKTGIEINIPA
                     ERSGGAPEPYRKIQRDKSMLRNHLKLIIDDAFKEDFEYDEDYKTEVIEEILSWMELEE
                     ELSRGEVIRRLESLGIDAEKKLPSEGRGLADIIKFDYLRFAGWNMLETLNVTIGQGMG
                     SYTPIQMANYIATIANGGNRHKLTLIDNIKNYNNSETVHKHEPQAEKIQLNDYDNLEH
                     IKKGMLMVTK"
BASE COUNT          641 a          309 c          386 g          392 t
ORIGIN      
        1 ctggatcatg atttaactgt agaagaaatc tttgatacat taataaaaaa ttatgaagta
       61 gacgaaaact taagtaaata tgaagccaga ggcatctttg gcctatataa ccaattgaaa
      121 aagcagggtt acctggccta tcagcctata aatatagcct atgggatcaa ggaaactacg
      181 gtggccaaaa tagaggaagg cctaatggag ttttccggca tagatgtatc catagaacca
      241 gtaaggtatt atccagaagg aactacagcg gcccatatcc taggctattt aggcaagata
      301 agccagccta atgaaattca aaaatacatt ggagaaaaga actactcccc tggtaccata
      361 ataggtaaaa cagggataga agaaagcttt gaagatcagt taagtggaga acacggcata
      421 aaaaggatag aggtagattc agtaggaaat accaccagtg taatttctga aatcaaaccg
      481 gtaccaggag acaacctcta cctgagtata gatttagacc tacagaaaaa ggcagaagag
      541 gtactagaac attccttaac tgaaatccaa aaaggtggca cctacaaaag tccttgggga
      601 gattataaat attctataaa tagaagcaaa aggcggccct ataaatacgc cacatctgga
      661 gctgtggtgg ctgtagatgt aaaaacagga caggtgctgg ccagtgccag tttcccagcc
      721 tatgatccta acttatttgc cacaggaatt acagattcag actggttaag tcttttccct
      781 gaaaatgagg atgacctact ggctccaagg cccttatata atatagcaac acagacagcc
      841 ttagaaccag gctccatctt taagatggcc acagccctaa cggccctaga aaagggacta
      901 agtccggaca aaaaaatccg ggacatgggt tatgttaatg taggtaagca gatgaattgt
      961 ctactgtgga ccatgagcag gaggacccat ggcaatgtaa atgtttatga ggccttgaga
     1021 gattcctgta actactattt ttactctttg gccctgggac aaaaccaaag gacaggtgag
     1081 tccataggca ccaagataga aatagaagac atagtagata tggtaaggaa attaggtatg
     1141 gatgataaaa ctggaataga gatcaatata ccagcagaaa gatcaggagg agctccagaa
     1201 ccctatagaa aaatccaaag ggacaagagc atgctaagaa accatctaaa gcttattata
     1261 gatgatgcct ttaaggaaga ctttgaatat gacgaagact ataaaactga agttatagaa
     1321 gaaatcttaa gctggatgga attggaagaa gagctaagtc gaggagaggt tataagaagg
     1381 cttgaatctt taggtataga tgcagaaaaa aaattaccca gtgaaggcag aggcctggca
     1441 gatattataa agtttgacta tctaaggttt gccggctgga atatgttgga gaccttaaac
     1501 gtcaccatag gccagggtat gggttcttac accccaatcc agatggccaa ctatattgca
     1561 accatagcca atggaggcaa caggcacaag ctgaccttga tagataatat taaaaattac
     1621 aacaattcag aaacagtgca taaacatgag cctcaagcag aaaagatcca gctaaacgac
     1681 tatgataatt tagaacacat caaaaagggt atgttaatgg taaccaag
//
LOCUS       DATGSI010000625         1197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_273812, whole genome shotgun sequence.
ACCESSION   DATGSI010000625 DATGSI010000000
VERSION     DATGSI010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273812"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VK031_09590"
     CDS             <1..349
                     /locus_tag="VK031_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54215.1"
                     /translation="IDKDRLVKNEINRLTRLFKDIDRNRRLTAKGLIEEAAYIKITLI
                     ELKEEIDKAGPIDEMPQGSYSILREHPALRSYNTMVQRYTNIIDKLVNLHPKEEIKKE
                     VDDGFDAFVASKP"
     gene            362..>1197
                     /locus_tag="VK031_09595"
     CDS             362..>1197
                     /locus_tag="VK031_09595"
                     /inference="COORDINATES: protein motif:HMM:NF015321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HLS54216.1"
                     /translation="MTAVRKIQYDLNYNPIIEYWNWINLNRQNRRRTSDKVHKVYNEL
                     VRIINDPKSEWEYDPAKGNHAIEFIENYCRHSKGKMGGKPFILELWQKALVAATFGMV
                     HKIDGTRKYQEVMLVVGRKNGKSTLAAAIGLYLQMADGEPGAEIYAVATKKDQAKIIW
                     LEAKRMVRKSPTLRKRMKTLVAEINSDFNDSFFRPLGRDSDSLDGLNVHGALLDEIHA
                     WKDVNLYDVVVDGTTAREEPLVFITTTAGTVRESVFDIKYDEAERVINGFEDPEGYKN
                     ER"
BASE COUNT          483 a          146 c          249 g          319 t
ORIGIN      
        1 aatagacaaa gatagattgg ttaaaaatga aataaataga cttactagat tatttaaaga
       61 tatagacaga aatagaagac tgactgcaaa aggccttata gaagaggctg catacattaa
      121 aattacactt atagaactta aagaggaaat agataaagct ggacctatag atgaaatgcc
      181 acaaggaagt tactctattc ttcgtgaaca tcctgcccta agaagttata atacaatggt
      241 gcaaagatat acaaatataa tagataagct agttaatttg catcctaaag aggaaattaa
      301 aaaagaagta gatgatggat ttgatgcatt tgtggcgagt aaaccatagg tgtgatatga
      361 aatgacagca gtaagaaaaa tacaatatga cttaaattat aatcccatta ttgaatattg
      421 gaattggata aaccttaata gacaaaacag aagaagaaca tctgataagg tccataaagt
      481 atataatgaa ttagttagaa ttattaatga tcctaaatca gaatgggagt atgacccagc
      541 taaagggaat catgcaatag aatttattga gaattactgt agacactcca aaggtaaaat
      601 gggaggaaag ccatttatat tagaactatg gcaaaaagct ttagttgctg caacctttgg
      661 aatggttcat aaaatagatg gtaccaggaa gtatcaggaa gtaatgttag ttgtaggtcg
      721 gaagaatggc aagtctacct tagctgctgc tataggatta tatctacaaa tggcagatgg
      781 agaaccagga gcagaaatat atgcagtagc cactaaaaaa gaccaggcta aaataatatg
      841 gttagaagct aaaagaatgg taagaaagtc ccctacactt aggaagagga tgaaaacttt
      901 agtagctgaa ataaatagtg attttaacga ttcttttttt agacccttag gtagagattc
      961 agattcacta gatggtctta acgttcatgg agcattactt gatgaaatac acgcatggaa
     1021 agatgtaaat ttatatgatg tagtggtaga tggtaccact gctagagaag aacctttagt
     1081 atttattact actacagcag gaacagttag ggaaagtgta tttgatataa aatatgatga
     1141 agctgaaaga gtaataaatg gattcgagga tccagaggga tataaaaacg aaagatt
//
LOCUS       DATGSI010000626         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_465856, whole genome shotgun sequence.
ACCESSION   DATGSI010000626 DATGSI010000000
VERSION     DATGSI010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465856"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..645)
                     /locus_tag="VK031_09600"
     CDS             complement(28..645)
                     /locus_tag="VK031_09600"
                     /inference="COORDINATES: protein motif:HMM:NF006777.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HLS54217.1"
                     /translation="MTKSGIKKIFSMGVWEDNPVFIQILGICSALAVTNLMLNSLIMG
                     LGLIFVTALSSFTISLIRQFTPKHIRMMVQVLIISAYVIMVDIFLKAYLPEMSKALGP
                     YVGLIITNCIIMGRAEAYAQQNPPIPSFLDGVASGVGYTIILLIIAFFRELMGFGTLF
                     GVQVLGDWWTPWSIMVMPPAAFFLLAIIIWVTHDMQNKDEEEVAK"
     gene            complement(649..>1094)
                     /locus_tag="VK031_09605"
     CDS             complement(649..>1094)
                     /locus_tag="VK031_09605"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HLS54218.1"
                     /translation="TPSDEPEDIDKTFDENIKEEKINGERVFIQEKDGEVVGYAIPTG
                     GAGLWGSIDAYVGISADLEHILGLEFIEHSETPELGGRISEEEYKGQFRDIDISSADE
                     GNYIIYRPSSGGNVDAITGATLTSESVRDFLNKDILKFIEEGEVE"
BASE COUNT          367 a          220 c          126 g          381 t
ORIGIN      
        1 taaatataat aaatggattt aattctatca tttcgccacc tcttcttcat ccttattttg
       61 catatcatgg gtaacccaaa ttataatagc taaaaggaag aaagctgctg gaggcattac
      121 cattatactc caaggagtcc accaatcacc aagtacctgt actccaaaca atgttccaaa
      181 acccataagt tctctaaaaa atgcaataat tagtaagata atagtatatc ctacaccaga
      241 tgctacacca tctaagaaag agggaattgg cggattttgt tgtgcatatg cttctgctct
      301 acccataata atacagttag taataattaa accaacataa ggtcctaaag ccttactcat
      361 ttctggtaaa taagctttta aaaatatatc taccattatt acatatgctg atataatcaa
      421 tacttgaacc atcattctaa tatgtttagg tgtaaattgt ctgataagag aaattgtaaa
      481 agaagaaaga gcagtaacaa atattaatcc taatcccata attaatgaat ttaacattag
      541 attagttaca gccaatgctg aacatatacc taatatttgt ataaaaactg gattatcttc
      601 ccaaacaccc attgaaaata tctttttaat accactttta gtcattgact attccacctc
      661 tccttcttca ataaatttaa gtatatcttt atttaaaaaa tctcttactg attcagaagt
      721 aagggtagca ccagtaatag catctacatt tccacctgat gatggtctat aaataatata
      781 atttccctca tctgctgaag atatatctat atctctaaat tgtcctttat attcctcttc
      841 tgaaatccta ccacctaatt ctggtgtttc agaatgttct ataaattcta atcctaatat
      901 atgttctaaa tctgcagata tacctacata agcatctata ctaccccata atcctgctcc
      961 tcctgttgga atagcatatc ctactacttc tccatccttt tcttgaataa agactctctc
     1021 tccgtttatt ttctcttcct ttatattttc atcaaatgtt ttgtctatat cttctggttc
     1081 atctgatgga gttg
//
LOCUS       DATGSI010000627         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_610562, whole genome shotgun sequence.
ACCESSION   DATGSI010000627 DATGSI010000000
VERSION     DATGSI010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610562"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VK031_09610"
     CDS             <1..438
                     /locus_tag="VK031_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLS54219.1"
                     /translation="LRIENTKIKTYKEAMKNMGGQPLVHRVDSHRIDGGYKAGPKLVE
                     ILQKHDATAVFCCQDELAIGLMNYMYDNNIKVPEDVSVVGYGDISVAGIYRPALTTIR
                     EPYYDIGAVAIRKILKELIGESIDDRNIELPVRLIKRKSSQRI"
     gene            608..>1139
                     /locus_tag="VK031_09615"
     CDS             608..>1139
                     /locus_tag="VK031_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HLS54220.1"
                     /translation="MEKIYIDGNSLTLTDFIKVARHNYKIDLTESAKEEVERSRRLVE
                     KFVNSNKTIYGITTGFGKFSEHIINKEESKRLQRNLIVSHACGVGHPLKEEYVRGIIL
                     LRINALAKGYSGIRLSTLNTLIEMLNKGIHPIIPEKGSLGASGDLAPLAHMALVFIGK
                     GEAIYKGQRMSGQEAME"
BASE COUNT          429 a          141 c          259 g          310 t
ORIGIN      
        1 ctaaggatag agaatacaaa gattaaaact tataaggaag ccatgaagaa catgggcggc
       61 cagcctttgg tccatagggt tgatagccac agaattgatg gaggatataa ggcaggccca
      121 aagctggtag aaattcttca aaaacatgat gctactgcag tgttttgttg ccaggatgaa
      181 ttggcaatag gactaatgaa ctatatgtat gataataata ttaaggtgcc tgaagatgtt
      241 tctgtagttg gctatggaga tattagtgtg gctggaattt atagacctgc acttactacc
      301 attagggagc cctattacga tataggagca gtagccataa gaaaaatttt aaaggaatta
      361 ataggcgaat caatagatga tagaaatatt gaactgcctg tgagactaat aaaaagaaaa
      421 agttctcaga gaatataaag atctaaaaat agaaattata gaaaagatta tggtgtaaga
      481 aggttgaatt gatgagaaag aatcttacaa gtataaaaaa attaagaagt gtataaaacc
      541 acagcatttg ctgtggtttt atacacttta cttaattaat atattagctt gatttggagg
      601 gaaagacttg gagaagattt atatagatgg aaatagcttg actttaactg attttataaa
      661 agttgccaga cataactata agatagactt aacagagtcg gctaaagaag aagtagaaag
      721 gtcaaggagg ctggtagaaa aatttgtaaa tagtaataaa acaatttatg gtattacaac
      781 cggatttgga aagtttagtg agcatataat taacaaggag gaaagtaagc ggttacaaag
      841 aaacctaata gtcagtcatg cctgcggggt aggacatccc ttaaaagaag aatatgtaag
      901 agggataatt ctacttagaa ttaatgcttt ggctaaagga tattcaggaa ttaggttatc
      961 aactttaaac accttaattg aaatgttaaa taaagggatt catcctataa tacctgaaaa
     1021 gggttcctta ggggctagtg gggatttagc acccttggcc catatggccc tagttttcat
     1081 cggtaaaggt gaggctattt acaagggaca aaggatgtct ggccaagagg caatggaaa
//
LOCUS       DATGSI010000628         1104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_185206, whole genome shotgun sequence.
ACCESSION   DATGSI010000628 DATGSI010000000
VERSION     DATGSI010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185206"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1104)
                     /locus_tag="VK031_09620"
     CDS             complement(<1..>1104)
                     /locus_tag="VK031_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54221.1"
                     /translation="NSSLNPIFITDETGNRRILQDYVKDITKDEFLENKELQNIIRKN
                     MSAEGLIDDLRRLNIKEVIPFLSSDIKGSVLQINNDFKTLYSLDDIFKAEDKQGKEVS
                     FEEAINAGVNISYAPTSSRRFAGKNDVLYEFNEDNEIGRFLKESLKTHSSNKLIGKYF
                     SSVVGTTLSDLPGYFGKMTEERNIALAERYLAAIDSYELTIVKGDEIFDELLSEVGKV
                     PSKHDKGYDLSSGTSITIRQNLADDQFVDAYMRVRDPGDRGNYNYVKVGEYDKSNIEN
                     IIGVPLTAHQGSPYISKPAPILSEDFGPDSLKGNGIFKMAATENKAERGVIIPASAKE
                     VSYERFKENPYAIEIFEDPRFKKEQEQIKGWYN"
BASE COUNT          277 a          221 c          157 g          449 t
ORIGIN      
        1 ttgttatacc atcctttgat ctgctcctgt tcttttttaa atctaggatc ttcaaatatc
       61 tctatagcat aaggattttc tttgaacctc tcgtatgaaa cttctttggc tgaggcaggt
      121 attattactc ctctttcagc cttattttca gtggctgcca tcttaaatat cccatttcct
      181 tttagggaat ctggtccgaa atcttcactg agtataggag caggcttact tatgtaagga
      241 gacccttgat gtgctgttaa aggtactcct attatatttt ctatgttact cttatcgtat
      301 tctcctactt ttacatagtt gtaatttcct ctatctcctg gatctcttac tctcatatat
      361 gcgtctacaa attgatcatc tgccagattc tgtctaatag tgatactagt tcctgaagat
      421 aagtcgtaac ctttatcatg tttagaaggc acttttccta cttcacttaa cagttcgtca
      481 aatatttcat ctccttttac tattgtcaat tcataagaat ctattgcggc taggtacctt
      541 tctgctagcg ctatatttct ctcttctgtc atttttccaa agtaccctgg taaatcacta
      601 agagtagtac ctactacgga tgaaaaatat ttcccaatta gcttgttgga agagtgtgtc
      661 tttaaagact cttttaaaaa ccttcctatt tcattatctt cgttgaattc gtatagtacg
      721 tcattttttc cagcaaatcg tctagacgat gtaggagcat aacttatatt cactcctgca
      781 tttatagctt cttcaaaact tacttctttc ccttgtttgt cttctgcttt aaatatgtca
      841 tctaaagaat ataaggtttt gaagtcgtta ttaatctgta gtacacttcc ttttatatct
      901 gaagataaaa agggtattac ttcttttata ttcaacctcc ttaagtcatc tatcaaacct
      961 tcagcagaca tgttttttct gattatgttt tggagctctt tattttccag aaattcatcc
     1021 ttggttatat cttttacata gtcttgtaat attcttctgt tgcctgtttc gtctgtaata
     1081 aatattgggt tcaagctact gttt
//
LOCUS       DATGSI010000629         1108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_89595, whole genome shotgun sequence.
ACCESSION   DATGSI010000629 DATGSI010000000
VERSION     DATGSI010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89595"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VK031_09625"
     CDS             <1..924
                     /locus_tag="VK031_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HLS54222.1"
                     /translation="SPDLVEGVIEYLHFHGYKNIVILEGSWVGDRTSRAFKACGYIEL
                     SEKYNIPLIDLQRDGFQEHQVGDIEVAICNEMMKLDYLINMPVLKGHCQTNITCALKN
                     LKGCIPDKEKRRFHTMGLHKPIALLNKVLKQDLIIVDGLIGDLNFEEGGNPVQMNRVI
                     VGKDPVMIDSYVAQLLGYDVEEVPYILMSKNLGIGDLVKEENIKELNKDSTPMTIPKS
                     RLVENLSQYVEENMACSACYGSLIHALDRLKYTGHLDKLPGKIHVGQGYERKIHSGIG
                     IGICTKGFDKSIPGCPPSAKSIIDFLTSLED"
BASE COUNT          354 a          177 c          238 g          339 t
ORIGIN      
        1 tctcctgatc tagtagaggg tgtaatagaa tatttgcatt ttcacggata taaaaatatt
       61 gtgatcttag aaggatcctg ggttggggac aggaccagca gggcctttaa ggcctgtggc
      121 tacatagagc tttctgaaaa atataatatt cccttgattg atctacagag ggatggcttt
      181 caagagcatc aggtaggaga tattgaagta gccatatgta atgaaatgat gaaactagat
      241 tacctgatta atatgcctgt tctaaagggt cattgccaga ctaatattac ctgtgccctt
      301 aaaaatctca aaggctgtat tcctgataaa gaaaaaagaa ggttccacac tatgggactg
      361 cacaaaccta tagccctttt gaataaggta ttgaaacaag accttattat tgtggatggg
      421 cttatagggg atttaaattt tgaggaaggt ggaaatccag tccagatgaa ccgagttata
      481 gtaggtaagg accctgtaat gatagatagc tatgtagccc aactattggg ctatgatgtg
      541 gaggaagttc cctacatttt aatgtccaaa aacctgggaa taggagattt agtgaaggaa
      601 gaaaatatta aggaactaaa caaagatagt acacctatga ccatacctaa aagccgttta
      661 gtggaaaacc taagccaata tgttgaggaa aatatggcct gttccgcctg ttatggtagt
      721 ttaatccatg ccttagatag gcttaagtat acaggtcatt tagataaatt acctggaaag
      781 atccatgtgg gacagggtta tgaaagaaaa atccattcag gtattggcat tggtatatgt
      841 accaagggct ttgataaatc aatacctggt tgtccaccta gtgccaaaag tattattgat
      901 tttttgacta gcttggaaga ctagttgtat aaaaacagtg ataaaagaac ccctagacct
      961 atatggatct aggggttttc ttaattcgtt ttttctgttt ctagattttc tatcacttct
     1021 ttaaactggg gtagatcctt tttatgtgct atcaacattt catctaacac agtcttgcct
     1081 attgttcctc caggaattaa tggattta
//
LOCUS       DATGSI010000630         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_139234, whole genome shotgun sequence.
ACCESSION   DATGSI010000630 DATGSI010000000
VERSION     DATGSI010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139234"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2182
                     /gene="nifJ"
                     /locus_tag="VK031_09630"
     CDS             <1..2182
                     /gene="nifJ"
                     /locus_tag="VK031_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HLS54223.1"
                     /translation="FSYDSKKSGGVTISHLRFGHEPIKSTYLISDADFISCSKQSYVY
                     QYDLLKGLKKGGTFLLNCIWDEEELDEHLPANLKRYIAENDINFYTINATKIASEVGL
                     GGRTNMVMQAAFFKLSEVLPLDEAIEHLKNSIVTAYGDKGEHIVEMNYAAVDRGIESL
                     VKVDVPEAWKDAEETEEKQDVPEFIREILEPMNRQEGDNLPVSAFVGREDGTFPHGTA
                     AYEKRAIAVEVPRWIPDHCIQCNQCSLVCPHATIRPFLLDEEEKENAPESFVTKKAIG
                     RGLENLEYKIQVSVLDCTGCGNCAEVCPSREKALVMVPFEEEYEVESKNWEYAMTIED
                     KGDLMNVNTIKGSQFRQPLLEFSGACAGCGETAYAKLITQLFGDRMYIANATGCSSIW
                     GGSAPSTPYCTNSEGKGPAWANSLFEDNAEFGYGMAIANRQIRDKIKRLMEEFLALEI
                     DSDLNEHFNDWIEGMNDAKASKAAAGMIIPRLDTDTGNEEANRILAEIKDRKDYLIKK
                     SIWIFGGDGWAYDIGFGGLDHVLASGEDVNVLVMDTEVYSNTGGQSSKATPTAAVAKF
                     AASGKKIRKKDLGLMMTTYGYVYVAQIAMGANMMHTIRAINEAESYDGPSLIIAYSPC
                     INHGIRTGMGTSLRQEKNAVDSGYWHLFRFDPRLKDQGKNPFVLDSREPKESFQDFIN
                     SEVRYTALKKRFPELADELFKAAEEDAKERYENYKRMARQYDEE"
     gene            2327..3241
                     /gene="murB"
                     /locus_tag="VK031_09635"
     CDS             2327..3241
                     /gene="murB"
                     /locus_tag="VK031_09635"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HLS54224.1"
                     /translation="MQTSDILKRLESKNVGEILIDEPMKKHTSFKIGGPVDIMLLPKN
                     EEQVVNGVSFCREHKIPYFIMGNGTNLLVRDGGIRGVVIKINRGFNKIEIDGGKIYSQ
                     AGAPLTVVSRKAKDNSLTGMEFANGIPGTIGGAVTMNAGAYGGEMKDIISKVRVLEPD
                     NQIKELSNEEMDFRYRGSKVIDEELIVLSVELQLKYGDKEKIEETMRDLNHRRTSKQP
                     LDLPSGGSTFKRPTGYYAGKLIDDSGLRGLRHGGAQVSEKHCGFVVNVDNATCKEVLD
                     LISVIKKTVKDKFGVDLEMEIKVIGEEA"
     gene            3242..>3456
                     /locus_tag="VK031_09640"
     CDS             3242..>3456
                     /locus_tag="VK031_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HLS54225.1"
                     /translation="MELYGDYHTHTIYSHGTGTIEDNVKYAIKKGLKEVAICDHGPAH
                     YLYGVKREKLPKMRDEIDRLNDKYSKH"
BASE COUNT         1266 a          517 c          790 g          883 t
ORIGIN      
        1 tttctcttat gatagtaaaa aatctggtgg agtaactatt tcccacttaa gatttggaca
       61 tgagccaatt aagtcaactt atttaatctc agatgctgac tttatatcct gttcaaaaca
      121 atcctacgta tatcaatatg atctactaaa gggattgaaa aaaggtggaa cattcctact
      181 aaactgtatc tgggatgaag aagaacttga tgaacactta ccagctaact taaagagata
      241 tattgctgaa aatgatatta acttctacac aataaatgct actaagatag ccagcgaagt
      301 aggcttaggc ggaagaacca acatggttat gcaggctgcc ttctttaagc tatcagaagt
      361 attgccacta gatgaagcaa ttgagcactt gaagaattca attgttacag cctatggcga
      421 taaaggtgag catattgtag agatgaacta tgcagcagtt gatagaggta ttgaatcact
      481 ggttaaagta gatgttccag aagcttggaa agacgcagaa gagactgaag aaaagcaaga
      541 tgtaccggag tttatcagag aaatcctaga accaatgaat aggcaagaag gagataattt
      601 acctgtaagt gcctttgttg gtagggaaga cggtactttc ccacatggaa cagctgcata
      661 tgagaaaaga gctattgctg ttgaagtacc acgttggatt ccagaccatt gtatccaatg
      721 taaccaatgt tcactagtat gtcctcacgc aactataaga ccattcttac tagatgaaga
      781 ggaaaaagaa aatgcaccag aaagctttgt aaccaagaag gctattggta gaggattaga
      841 gaacttagag tacaagattc aggtttcagt actagactgt acaggttgtg gaaactgtgc
      901 agaagtatgt ccttcaagag aaaaggcact agttatggta ccatttgaag aggaatacga
      961 agtagagtcc aaaaactggg agtatgccat gactattgag gataagggcg acctaatgaa
     1021 tgtaaacact attaaaggta gccagtttag acaaccactt cttgaattct ctggagcttg
     1081 tgcaggatgt ggagagacag catacgctaa attaattact caactatttg gagatagaat
     1141 gtatatagcc aatgctacag gttgttcatc aatctggggt gggtcagcac cttcaacacc
     1201 atactgtaca aatagcgaag gtaaaggacc tgcatgggca aactcactgt ttgaagacaa
     1261 cgccgagttt ggctacggta tggctattgc taatagacaa ataagagata aaatcaaacg
     1321 cttaatggaa gaattcctag cattagaaat agattctgac ttaaatgagc atttcaatga
     1381 ttggatagag ggaatgaatg atgcaaaggc gtcaaaagct gcagcaggta tgataatacc
     1441 aagattagac actgacacag gaaatgaaga agcaaataga atactagcag aaataaaaga
     1501 taggaaagac tatctaatca aaaaatcaat ctggatcttt ggtggagacg gctgggccta
     1561 tgatatagga ttcggtggat tagaccacgt gctagcatca ggtgaagatg taaacgtact
     1621 agtaatggac acagaggtat actcaaatac tggtggtcaa tcatctaaag ctactccaac
     1681 agcagcagtt gcgaaattcg cggcttctgg taagaagatt aggaagaaag acttaggtct
     1741 gatgatgaca acatatggtt atgtatatgt agcacaaata gccatgggtg ccaatatgat
     1801 gcatacaatt agagcaatca atgaagcaga gtcttatgac ggaccatccc taatcatagc
     1861 ttattcacca tgtataaacc atggtataag aactggtatg ggtactagct taagacaaga
     1921 aaagaatgca gtagattcag gttactggca tctgtttaga ttcgacccaa gattaaaaga
     1981 tcaaggaaag aatccattcg tcttagattc aagagagcct aaggaatcct tccaagactt
     2041 cataaattca gaggttagat acactgccct gaagaagaga ttcccagagc tagctgacga
     2101 gttatttaaa gcagcagaag aagatgctaa ggaaagatac gaaaactaca agagaatggc
     2161 aagacaatac gacgaagaat aatttataaa aaaggttatc agttccggct gataaccttt
     2221 taaatttttc aacttgatca atcaaattat taataatatt gctcgtgtgg tataaatata
     2281 tattatatga taaaatatgt ggagaatact gtttaggagt gatattttgc aaacttcaga
     2341 tatactaaaa agacttgaaa gtaaaaatgt gggggaaata ttaatagatg aaccaatgaa
     2401 gaaacacaca tcctttaaaa taggtggacc agtagacata atgttgctac ctaaaaatga
     2461 ggagcaggta gttaatgggg taagcttttg cagggaacac aaaatccctt attttattat
     2521 gggaaatgga acaaatctat tagtcagaga tggtggaatc aggggtgttg taattaagat
     2581 aaataggggc tttaacaaga tagaaataga tgggggaaaa atatatagcc aggcgggagc
     2641 acccttgact gtagtcagta gaaaggctaa agataactcc ttaacaggta tggaatttgc
     2701 taatggtata ccaggcacca taggtggggc tgttactatg aatgcaggag cctatggtgg
     2761 ggaaatgaag gatataataa gcaaggttcg ggtactggag cctgataatc agataaaaga
     2821 attaagcaat gaagaaatgg actttagata tagaggtagc aaggtaatag atgaagaact
     2881 tatagtccta agtgtagagt tgcaattaaa atatggtgac aaggaaaaaa tagaggaaac
     2941 catgcgggat ttaaatcata gaaggacttc aaaacagcct ttagatctgc ccagtggtgg
     3001 cagcaccttt aaaaggccta caggttacta tgctggaaaa ttaattgatg attcagggct
     3061 taggggtctg agacatggtg gagctcaggt atcagaaaaa cattgtggct ttgttgttaa
     3121 tgttgacaat gcaacatgta aggaagtttt agacctaata tcggttatta aaaagacagt
     3181 aaaagataag tttggtgtag atctggaaat ggaaataaag gttataggag aagaggccta
     3241 gatggaactt tatggagact atcacactca tacaatctat agtcatggga ctggtaccat
     3301 agaagacaat gtaaaatatg ctattaaaaa aggcttaaag gaagtggcta tttgtgatca
     3361 tgggccagcc cattacctat atggagtaaa gagagagaaa ttgcccaaaa tgagggatga
     3421 gatagacaga ttaaatgaca aatattctaa acataa
//
LOCUS       DATGSI010000631         1226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_610791, whole genome shotgun sequence.
ACCESSION   DATGSI010000631 DATGSI010000000
VERSION     DATGSI010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610791"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VK031_09645"
     CDS             <1..695
                     /locus_tag="VK031_09645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLS54226.1"
                     /translation="ATKVYKRFDRIFTVSEEAREKLVKMVPSINDKTKVFTNMISPEL
                     IIEEAKMGVGFQQDNFTGIRIVTVGRLAFEKGQDLAIKAMVKLIKDGINVKWYCIGEG
                     KGRKEYEELIDRYQLQDKFILLGENSNPYRFIKECDIYVQPSRHEGYCITLAEARALD
                     RPIVTTSFTGAKEQVKHNETGLVVSIHEDQIYYAVKGLIYNPKQREKFSRNLSNEMFE
                     SIGRIGRIYNIV"
     gene            798..>1226
                     /locus_tag="VK031_09650"
     CDS             798..>1226
                     /locus_tag="VK031_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54227.1"
                     /translation="MKKIVFVTERMIMGGIEKSLISKLEKIPKDRYEVTVLLMGNNGE
                     LLDRIPKNVIVKNTFGIEGTTIKRMKGNIKKGRPITAFKVGWYTWLIQRTKSRFDQNR
                     YYSKIAMAEDTEYDLAIAYHGPVSFPVKHVIENITAKRKIA"
BASE COUNT          476 a          157 c          263 g          330 t
ORIGIN      
        1 ttgcaactaa ggtatataag aggtttgata ggatatttac tgtttcagaa gaagcgaggg
       61 agaaattagt aaaaatggtc ccgtctataa atgataaaac aaaggtattt actaatatga
      121 tatctcccga gttaattatt gaagaagcta aaatgggagt aggctttcaa caggataact
      181 ttacaggaat taggatcgta acagtaggta ggctagcatt tgaaaaaggg caagatttgg
      241 ctattaaagc aatggtaaag ttaataaagg atggaatcaa tgtcaaatgg tattgcatcg
      301 gtgaggggaa aggtaggaaa gaatatgagg aactaattga tcgttatcaa ctgcaagata
      361 aatttattct tttgggggaa aattctaatc cgtataggtt tataaaagaa tgcgatatat
      421 acgtacaacc atccagacat gagggatatt gtattacact ggctgaggca agggccctag
      481 acagacctat tgttacaact agctttactg gggcaaaaga gcaagtaaaa cataatgaga
      541 cggggctagt tgtaagtata catgaggacc aaatttacta tgctgttaaa ggtctaatat
      601 acaacccaaa acaacgcgag aaatttagta ggaatttatc aaatgaaatg tttgaaagta
      661 tcggtagaat aggccgtatt tataatattg tttaggagaa atatggttta tcatcttaat
      721 gcaatcgtaa aagacgaata atcggcgata aatactagac tattcagtgt tataaggaag
      781 gtgaaccgta tatacagatg aaaaagattg tatttgtaac tgaaagaatg attatgggtg
      841 gtatagaaaa atccctgatt tccaaactag aaaaaatccc aaaagacaga tatgaagtga
      901 cagttttact aatgggaaat aatggggaac tcttagacag gatccctaaa aatgtcatag
      961 taaagaatac ttttggtatc gaaggaacta caattaaaag aatgaaaggt aatataaaaa
     1021 aaggcagacc aatcaccgct ttcaaggtgg gatggtacac atggttaata caaagaacaa
     1081 aatcaaggtt tgaccaaaat aggtattatt ctaaaatagc catggctgaa gataccgaat
     1141 atgatttggc tatcgcatat catggtccag tatcatttcc cgtaaagcat gtaatagaaa
     1201 atattactgc taaaaggaaa atagct
//
LOCUS       DATGSI010000632         1157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_563390, whole genome shotgun sequence.
ACCESSION   DATGSI010000632 DATGSI010000000
VERSION     DATGSI010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563390"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     repeat_region   2..300
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2..31
                     /rpt_unit_seq="gttattagtctacctataaggaattgaaac"
     gene            504..>1157
                     /gene="cas6"
                     /locus_tag="VK031_09655"
     CDS             504..>1157
                     /gene="cas6"
                     /locus_tag="VK031_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HLS54228.1"
                     /translation="MKETIILESVEQDREIILPIDYNHMVQAMIYNQLEEEVADFLHE
                     EGFRKEKRTYKLFTFSRLLGRFRLDRKKGQIKFIGPIKLIISSPYNDFNNSIGNNILT
                     NKYIRLGNNRLVAKELSVKKERVENQQITIDALSPITVYSTLLREDGRKFTYYFNPKE
                     EDFSRIISENLRNKYKAFYGTEPPKGEVIIESKGRSKMSIINYKGFIIKGYMGRFTML
                     "
BASE COUNT          453 a          160 c          205 g          339 t
ORIGIN      
        1 tgttattagt ctacctataa ggaattgaaa catatctgca aaatcaaaga ctcttggaga
       61 tacattatag ttattagtct acctataagg aattgaaacg gatatctata tactactata
      121 cctccaccat tccatgggtt attagtctac ctataaggaa ttgaaactga aactgcttgg
      181 gataatcatt tgaataaatt agggttatta gtctacctat aaggaattga aaccactgtt
      241 atgcgtgctt gagtatatcc ttgatcctaa gttattagtc tacctataag gaattgaaac
      301 caccgacacc ttcagagaaa aagactaagt atataatcaa tagactacct aaaagaaata
      361 ataaaaatgc ttaaaagccc tatgacctac ttgaaggtca taggtttttt taaaaaaaca
      421 ataaaaaaga aggacttttt aaaaacgtgt agaatatagt aaataagaaa ttatttaatt
      481 ggtgtatgag gggagaccta attttgaaag agacgattat acttgaatca gttgaacaag
      541 atagagaaat tattcttcca attgactaca accatatggt acaagccatg atctataacc
      601 agttggagga agaagtggcc gactttttac atgaggaagg gtttagaaaa gaaaaaagaa
      661 cttataaact atttactttt tctcggttac taggaagatt tagattagac aggaaaaaag
      721 ggcagattaa atttataggc cccattaagc taattatttc ttcaccatac aatgatttta
      781 ataactccat aggaaataat atactaacca ataaatacat taggctagga aataataggt
      841 tagtggcgaa agaattgtct gttaagaaag agagagtaga aaatcagcaa ataactattg
      901 atgcactatc tcccataaca gtttatagta ccctgcttag agaggatggg agaaagttta
      961 catattattt caatcctaaa gaagaagact tttctagaat aattagtgaa aacctaagaa
     1021 ataaatataa ggccttctat ggcacagaac ctccaaaagg agaggtaatt attgagtcta
     1081 aagggagaag taaaatgtcc ataattaatt ataagggctt tatcataaag ggatatatgg
     1141 gcaggtttac tatgtta
//
LOCUS       DATGSI010000633         3434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_610817, whole genome shotgun sequence.
ACCESSION   DATGSI010000633 DATGSI010000000
VERSION     DATGSI010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610817"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VK031_09660"
     CDS             complement(<1..954)
                     /locus_tag="VK031_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HLS54229.1"
                     /translation="MKNLTPMMKQYMEIKSRYKDSILFFRLGDFYEMFFEDAITASRE
                     LEITLTQRDCGMEEKAPMCGVPHHVADTYVARLVAKGYKVAICEQVEDASEAKGLVRR
                     DVIRVVTPGTITDDNVLEEKSNNFLTSIYFDDLGVGISYVDNSTGEMYTTEYSGYTVE
                     DNYRFVLDELGKISPAEIICNEKFMSDSNYINIINNKINPFFNIYKDSAQARTKLGGK
                     ITNLFDGKNFKDLNIEGKIYSILATDKLIEYLYETQKNSLDHINQLIYYKPYEYMVLD
                     INTRTNLELHETIMSKQKKGALIHVIDKTSTAMGGRLLKKWL"
     gene            complement(968..2293)
                     /gene="miaB"
                     /locus_tag="VK031_09665"
     CDS             complement(968..2293)
                     /gene="miaB"
                     /locus_tag="VK031_09665"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HLS54230.1"
                     /translation="MKKYMIQTYGCQMNEHDSEKISWILEGMGYVLTDNREECDLIIY
                     NTCAVRKSAEDRVYGQLGELKELKRRNPDLILSVCGCMMQREEAIEIITSKYKHVDII
                     FGTNNIHKLPQLVNNYKKTGQTIIDIVEDSREIVESIDANRRYDFKAFVNITYGCNNY
                     CSYCIVPYTRGRERSRKPEHIIEEVKDLASKGCKEVTLLGQNVNSYGNTFDNDYNFAD
                     LLRDINEINGIERIRFMTSHPKDISDELISLFGELDKLCDHLHLPVQSGNNRVLRYMN
                     RRYTREDYLEIIKKIRKVNPDISLTTDIIVGFPGETEEEFEDTIKLVEEVEFDSAYTF
                     LYSVREGTPAAKFKDQMDHKTKHERFQRLLKVLHPIGLEKNKRLVGKTLPVLVEEVSK
                     NDEKILNGRTTSGKLVHFEGDKDLIGKILDVKIENGKTFTLEGKIIDLE"
     gene            complement(2437..3024)
                     /locus_tag="VK031_09670"
     CDS             complement(2437..3024)
                     /locus_tag="VK031_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HLS54231.1"
                     /translation="MLDYNVEYSISEAAEIIGYAPHVLRYYEKEFEIDIPRKDSNHRY
                     YTYKEIELMQYIKSLKDKGFANKQIKMIINSPETILSQDDTNTGLVKSQAQVVKPDDM
                     AILISEALEERFFEKLAQHISNHSDDTSKLIEDLKEEIVNLRNEINSQERDVLICENA
                     KLKMKVKEKTYEIVRLKESLLRSQEKKGLFRKIFG"
     gene            complement(3195..>3434)
                     /locus_tag="VK031_09675"
     CDS             complement(3195..>3434)
                     /locus_tag="VK031_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HLS54232.1"
                     /translation="YVRGTLEDLAKKKDIQYQLEVLEFGGTDSGAIHVTRGGVPAGVL
                     SIPTRYVHSAIEMAAKNDINSCSKLLVEFLNYNFE"
BASE COUNT         1030 a          708 c          442 g         1254 t
ORIGIN      
        1 tagccatttt ttcagtagcc gtccccccat agccgttgat gttttatcaa taacatggat
       61 taaggccccc tttttctgct ttgacattat ggtttcatgt agctctaagt tcgtcctagt
      121 attaatatct aaaaccatat actcataggg cttgtagtat attaactggt ttatatgatc
      181 taaagagttt ttttgtgttt cgtataaata ctctattagt ttatcagtgg ctaaaatgga
      241 atagatttta ccctctatgt ttaggtcctt aaaatttttt ccatcaaata ggttagttat
      301 tttgccacct aatttagtcc tagcttgagc actgtcctta taaatattaa agaaggggtt
      361 tattttgtta ttgataatgt ttatatagtt ggaatcagac ataaattttt cattacatat
      421 aatctctgca ggcgaaatct tacctaactc atcaagaaca aacctataat tatcttccac
      481 agtatagcct gaatattctg tagtatacat ttcaccagta gaattatcta cataacttat
      541 acctacacca aggtcatcaa aatagataga agtgagaaaa ttattagact tttcttccaa
      601 aacattatca tcggtaatag taccaggagt taccaccctt ataacgtctc ttcttaccag
      661 gcccttagcc tctgaggcat cttccacttg ctcacatatg gcaaccttat atccctttgc
      721 cactagtctt gccacataag tatcagctac gtgatgggga accccgcaca taggtgcctt
      781 ttcttccata ccgcaatccc tttgggtaag agtaatttcc agctcacggg atgctgtaat
      841 agcatcctca aaaaacattt catagaaatc acctaaccta aagaataaga tagagtcctt
      901 atatctagat tttatttcca tatattgctt catcataggt gttaaatttt tcaaattata
      961 acctccgtta ttcaaggtct attatttttc cttccaaagt aaaggtctta ccattttcga
     1021 ttttaacatc caaaatcttc ccgattaagt ccttgtctcc ctcaaaatga accagtttgc
     1081 cactggtagt tctaccattt aagatctttt catcattctt gcttacctct tcaactagaa
     1141 ccggcaaggt tttgcctact aatcttttat ttttttctaa cccaattggg tgcagaacct
     1201 taagcaacct ttggaatctt tcatgtttag tcttatgatc catctgatcc ttaaactttg
     1261 cagcaggtgt gccttcccta actgaataaa ggaaggtgta ggcagaatca aactctacct
     1321 cctctacaag cttaatggta tcctcaaact cctcttcagt ctcacctggg aagcctacaa
     1381 ttatatcagt ggttaaacta atatcaggat taacctttct tattttcttt atgatttcta
     1441 gataatcttc cctggtatac cttcggttca tatatcttag aaccctatta tttccagact
     1501 gtaccggcaa atgtagatga tcacataatt tatctaattc accaaacaag ctgattaatt
     1561 catcagaaat atctttagga tgggatgtca taaatcttat tctttctatt ccattaatct
     1621 catttatatc tcttaacaaa tcagcaaaat tatagtcatt atcaaaggta tttccatagg
     1681 agtttacatt ttgaccaagt aatgttactt ccttgcaccc ctttgaggcc aggtccttaa
     1741 cttcttcaat aatatgttca ggctttcggc tcctttccct acctctagta tatggtacta
     1801 tacaatagct gcaatagtta ttacagccat atgttatgtt tacaaaggcc ttaaaatcat
     1861 accttctatt agcgtcaatg ctttccacaa tttccctact atcctcaact atatctataa
     1921 ttgtttggcc tgttttttta tagttattta ccaactgagg cagcttgtgt atattatttg
     1981 ttccaaatat aatgtccaca tgcttatatt tagaagtaat tatctctata gcctcttccc
     2041 tttgcatcat acagccacag acacttaaaa taaggtcagg atttctacgc tttaattctt
     2101 ttagttctcc cagctgacca tagaccctat cttcagctga ttttctaact gcacaagtat
     2161 tatatattat aagatcacat tcttccctat tgtcagttag aacataaccc ataccttcaa
     2221 gaatccaaga aattttttct gaatcatgtt cgttcatttg acaaccatag gtctgaatca
     2281 tatatttctt catttatgtt aattcccctc tttctaattt tatatgacta ttataacaaa
     2341 taatattgaa aaataaagga ctagcctatt aaactattat accatattat aaataaaaaa
     2401 aggctagtcg gcaaaactaa ccttttacaa gcattactat ccaaaaattt tcctaaaaag
     2461 ccccttcttc tcctggcttc ttaggagact ttcctttaat cttacgattt cataagtttt
     2521 ttcttttact ttcatcttca actttgcatt ttcacatatt aatacatctc tttcttgaga
     2581 gttaatttca tttctcaaat ttactatttc ttcctttaaa tcctctatta atttagatgt
     2641 atcatcactg tgattggaaa tatgttgggc taacttttca aaaaatcttt cctctaaggc
     2701 ctcagaaatt aatatagcca tatcatcagg cttaactact tgggcttggc ttttcactaa
     2761 acctgtgttc gtatcatctt gagataaaat cgtttcagga gaattgatga tcatttttat
     2821 ttgcttatta gcaaaacctt tatcttttag gcttttaata tattgcatta actcaatttc
     2881 tttataggta tagtagcgat ggtttgagtc ctttcttggg atatctatct caaattcctt
     2941 ctcataatat cttaatacat gtggcgcata ccctataatt tctgcggcct cagatatact
     3001 atattctaca ttgtaatcta acaagacaac atcccctcca caacaaatca aaatagctat
     3061 atactataga tttctctaga ggatgtataa aatcctttat ttattaaaaa gcaaattact
     3121 acaacttcct atataaattg gcaaaaaatg ataaaaagat ctgtaaaaat aaggaaggct
     3181 gttttcagcc ttctttattc aaaattatag tttaaaaatt ctactaataa cttgctacaa
     3241 ctatttatat catttttagc agccatttct atagctgaat gaacatatct ggtaggtata
     3301 gatagtactc cggcagggac ccctcctcta gttacatgga tagcacctga gtctgtacca
     3361 ccaaattcca atacctctaa ttggtactga atatcctttt tcttagctag gtcttccaag
     3421 gtgcctctaa cata
//
LOCUS       DATGSI010000634         1121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_704659, whole genome shotgun sequence.
ACCESSION   DATGSI010000634 DATGSI010000000
VERSION     DATGSI010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704659"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VK031_09680"
     CDS             complement(<1..631)
                     /locus_tag="VK031_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54233.1"
                     /translation="MQYNNLTVLISTIVEFCNKHSMINSSSVRNEVNFRAIPDTDYPT
                     VNIEPVEAPISGNQISQGFVITVADLMNTDFPNYNTYHLIDNSQRLAQDIINYLQNVE
                     EFEASPNVTLNPFEDEGTDSTAGVVFRINITYTRDTNICVFDDLVNIERPFCFNEDLR
                     TVTFCKWLMYPYLDQYEVTFDGGDNWITMDRFTYDIGDEEIPVKTIGQRI"
     gene            complement(643..>1121)
                     /locus_tag="VK031_09685"
     CDS             complement(643..>1121)
                     /locus_tag="VK031_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54234.1"
                     /translation="KSLDDKILRVTVYEVQDLELKPIIGKELYKSLEEEVKKKNDDSS
                     YVIPDKFLDLLDVIRPFISYGVLVHLVVPITYKATNKGFAVKNDTNASIKEGSDLQFV
                     MNYYKTKFDAYKKRLTEEVGKHCNSFMEKDLGWTTGWYIQNNQKRRERIERAVNKY"
BASE COUNT          338 a          213 c          153 g          417 t
ORIGIN      
        1 taattctttg tcctattgtt tttactggta tttcttcatc ccctatatca taggtaaacc
       61 tatccatagt aatccagtta tctcctccat caaatgttac ctcgtactgg tctaagtaag
      121 ggtacataag ccatttacag aaagtaactg ttctaaggtc ttcgttaaag cagaaaggtc
      181 tttctatgtt aaccaaatca tcaaatacac agatattagt gtctctggta taagttatat
      241 taattctaaa gactacccct gctgtactgt ctgtaccttc atcttcaaaa ggatttaaag
      301 taacattagg agaagcttca aactcctcta cgttttgtaa gtagtttata atatcctgtg
      361 ccaatctttg ggaattatct ataagatggt aagtattata attagggaag tcagtattca
      421 ttaagtcagc tacggtaata acaaacccct gtgaaatctg atttccagat ataggtgctt
      481 ctactggttc tatatttaca gtagggtagt cagtgtctgg aatagctcta aaattaactt
      541 cattcctaac tgaactacta tttatcatag aatgtttgtt acagaactct actatggttg
      601 atataagtac tgttaaattg ttatactgca ttatatttct aattaatatt tatttactgc
      661 cctttctatt ctctctcttc ttttctggtt attctgtata taccatccag tagtccaacc
      721 taggtctttc tccataaaag aattacagtg tttaccaacc tcttctgtta acctcttctt
      781 ataggcatca aacttagttt tataatagtt cattacaaac tgtaaatctg agccttcttt
      841 tatagaagca ttggtatcgt tcttaacagc gaacccttta ttagtagctt tataggttat
      901 gggaacaact aagtgaacta atactccata ggatatgaag ggtcttataa catctaataa
      961 atctaagaac ttatcaggga taacatagct actatcatca ttcttcttct taacctcttc
     1021 ctctaatgat ttatataact ctttacctat aataggcttt aactctaaat cctgtacctc
     1081 atatacagta acccttagaa ttttgtcatc cagacttttt t
//
LOCUS       DATGSI010000635         1727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_274313, whole genome shotgun sequence.
ACCESSION   DATGSI010000635 DATGSI010000000
VERSION     DATGSI010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274313"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VK031_09690"
     CDS             <1..349
                     /locus_tag="VK031_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HLS54235.1"
                     /translation="RGIVSYSNQSKIEELKVKKSTLKTYGAVSNKTAYEMAKGLLNKT
                     NIDIALSVTGIAGPGGGSAEKPVGTVFVCVMSNKDYMVEEYRLNGNRIMIQNRTTTRA
                     LNLLRKFILDNRY"
     gene            437..1477
                     /gene="recA"
                     /locus_tag="VK031_09695"
     CDS             437..1477
                     /gene="recA"
                     /locus_tag="VK031_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HLS54236.1"
                     /translation="MTDMKEKKKALDLALNQIEKQFGKGSIMRLGEDVKMELDFIPTG
                     SLDLDIALGIGGVPRGRIIEVFGPESSGKTTVALHMIAEAQKLGGIAAFIDAEHALDP
                     GYAKRLGVDIENLVISQPDTGEEALEIAEALTRSGAIDIVVVDSVAALVPKAEIEGEM
                     GDSHIGLQARLMSQALRKLAGAISKSNTTCIFINQLREKVGVMFGNPETTSGGRALKF
                     YSSIRLDVRRIDTIKQGDEILGNRTRVKIVKNKVAPPFKQAEFDIMYGKGISKEGNIL
                     DAGVTAEVVRKAGSWYSYGDIRLGQGRENSKDFLRDNPEICLEIENKVREKYGLTKED
                     DKSEEDSEESQD"
     gene            1533..>1727
                     /locus_tag="VK031_09700"
     CDS             1533..>1727
                     /locus_tag="VK031_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLS54237.1"
                     /translation="MKLLFFTDTHIRGTTPKNRKDDFPEALERKLYEVLDLINKENID
                     YVLHGGDLFDRPDLSVSIVSR"
BASE COUNT          616 a          243 c          408 g          460 t
ORIGIN      
        1 aaggggtatt gtaagctata gtaatcagtc taaaattgaa gaattaaagg ttaaaaaatc
       61 tactttaaaa acatatgggg ctgtaagcaa taagacagcc tatgagatgg ccaaaggcct
      121 gctcaataaa acaaatatag atatagcctt atctgtaaca gggattgctg ggccaggtgg
      181 aggtagtgcg gaaaagccag taggtaccgt atttgtttgc gtaatgagca ataaggatta
      241 tatggtagaa gaatatagat taaatggtaa caggataatg attcaaaacc ggactaccac
      301 cagagcacta aatctactga gaaagtttat attagacaat aggtattgac taaatatggt
      361 taatattata ttataaatat acaaaactaa cgttcgagca tgagagaggg gtgaatccag
      421 tttaactgga aaagttatga ctgatatgaa agaaaaaaag aaggctttag acctagcctt
      481 gaatcaaatt gaaaagcaat ttggaaaggg atccatcatg agactgggtg aagatgttaa
      541 aatggagtta gattttatcc caacaggatc cctagattta gatattgccc taggaatagg
      601 tggagttcca agagggagga ttattgaggt atttggacca gaatcatctg gtaaaacaac
      661 agttgcatta catatgattg cagaggccca aaaattaggc ggcatagctg cattcataga
      721 tgctgagcac gcactagacc ctggatatgc taaaagatta ggggtggata ttgaaaatct
      781 ggttatttca caacctgata caggtgagga ggctctagag attgcagagg ctttaaccag
      841 aagtggtgcc atagacatag tagtggtaga ctcagtggca gccctggttc ctaaagctga
      901 aatagaaggg gaaatgggtg atagccatat tggactacag gctagattaa tgtctcaggc
      961 tttaaggaaa ctggcaggag caattagtaa gtccaataca acctgtatct ttataaatca
     1021 gcttagggaa aaggttggag ttatgtttgg aaaccctgaa actacttcag ggggacgggc
     1081 acttaagttc tattccagta ttagattaga tgttagaaga atagatacaa ttaaacaggg
     1141 tgatgaaatc ttaggaaata gaactagggt aaagattgtt aaaaacaagg tggcacctcc
     1201 ctttaaacag gcagaatttg atattatgta tggcaagggc atatctaagg aaggcaatat
     1261 tttagatgct ggagttactg ccgaagtagt tagaaaggca ggctcttggt atagctatgg
     1321 tgatataagg ctaggtcaag gtagggagaa ctctaaggac tttctaagag acaatcctga
     1381 aatatgctta gaaattgaaa ataaagtcag agaaaagtac ggattaacta aagaggatga
     1441 caagtccgaa gaggattccg aagaatccca agattaacta cttttcattt aaaacctcct
     1501 gttaaggagg ttttgtttta ggagtgaaaa ctatgaaact attattcttt actgatacac
     1561 atatacgggg tacaacaccc aaaaatcgaa aagatgattt tccagaggcc ctagaaagaa
     1621 aattatatga ggttcttgat ttaattaata aggagaatat agactatgtt ctccacggcg
     1681 gtgatctgtt tgataggcca gatttatctg tatctattgt aagtaga
//
LOCUS       DATGSI010000636         3287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_185594, whole genome shotgun sequence.
ACCESSION   DATGSI010000636 DATGSI010000000
VERSION     DATGSI010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185594"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..304
                     /gene="rpsO"
                     /locus_tag="VK031_09705"
     CDS             38..304
                     /gene="rpsO"
                     /locus_tag="VK031_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HLS54238.1"
                     /translation="MLSKETKEQIIKEYGLHEGDTGSPEVQIALLTHRINSVNEHLKT
                     HKKDHHSRRGLLKMVGQRRGLLRYLREKDIVRYRSLIEKLNIRG"
     gene            460..2553
                     /locus_tag="VK031_09710"
     CDS             460..2553
                     /locus_tag="VK031_09710"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HLS54239.1"
                     /translation="MEKKFSYDLAGRELTVTIGKVAEQAGGACLIQYGDTSILVTATS
                     SKEPREGIDFFPLSVDYEERLYSVGKIPGGFIKREGRPSEKAILTSRLIDRPIRPLFP
                     EGYRNDVQVIATVLSVDQDHSPEIAAMIGSSVALSISDIPFDGPTGSVLVGLVDGKFI
                     INPNSEERDKSDIHLIVSGTKDAIMMVEAGADEVPEETILEGILFAHEEIKNICDFIE
                     GIANEVGKEKQDYQVFMPDKELEEKIIEYGTEKLIKAINTVEKQEREDKIDLVKDEIL
                     EKFIEEYPDNEKDILAVIDTIIKKEVRKLISEKNLRPDNRQPDEIREITSEVGLLPRT
                     HGSGLFTRGQTQVLTIATLGASSDVQIIDGLGEEESKRYMHHYNFPPYSVGETRMLRG
                     PGRREIGHGALAERALEPVIPSEEEFPYTIRLVSEVLSSNGSSSQASVCGSTLSLLDA
                     GVPIKASVAGIAMGLIKEGDKVTILSDILGMEDHLGDMDFKVAGTRKGITAIQMDIKI
                     PGIDREILEEALEKAREGRLYILDKMEETISEPRKELSPYAPKIFTMQIDPEKIRDVI
                     GPGGKMINKIIDETGAKIDIDDDGQIFIATSDSDSGERAKEMIESIVKEAEVGDVYLG
                     KVTKITNFGAFVEILNGKEGLLHISNIARERINRVEDVLKVGDDVLVKVIGIDSKGIS
                     LSRKALLPDPDKKEE"
     gene            2662..>3287
                     /locus_tag="VK031_09715"
     CDS             2662..>3287
                     /locus_tag="VK031_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLS54240.1"
                     /translation="MRLIILNKRTLIILVSLALVVLLILAFTLGRFRSKEVFNPDLFY
                     QGTTEEKLVAFACNIDWGGEYLEEMLAILDINEIKITFFPTGRWAEKNEALLKLIYDK
                     QHEIGNHGYNHLDYDKLDEANNYREIKTAHEIIEKIINDQPKYFAPPSGAFNEHTISA
                     AKQLGYKVILWSIDTIDWREDSNKDVIYQRVIEKLHNGGIVLMHPTEET"
BASE COUNT         1254 a          449 c          713 g          871 t
ORIGIN      
        1 tagctttagg tgtatacaaa atatggaggt gcaaataatg ttatctaagg aaacaaagga
       61 acaaattatt aaggagtatg gactacatga aggtgacaca ggttcacctg aggtgcaaat
      121 cgcactttta actcatagaa ttaattccgt taacgaacat cttaagactc acaaaaaaga
      181 tcaccattct agaaggggac tattaaagat ggttggtcaa agaaggggac tactaagata
      241 cttaagggaa aaagatattg taagatatcg ttctttaatt gagaaactaa acattagagg
      301 ttaataagag cgggaaagtc ccgctcttta gtttataaaa gggtaaaatt aggcctcata
      361 taaaattcac taaaaataaa atatgataaa gtgatattag aaatattgag ggatataata
      421 tttatagtgt tacaaaagag aaaagcaagg aggtattgta tggagaagaa gtttagttat
      481 gatttagcag gtagagaact aactgttaca ataggaaaag ttgctgaaca agcaggaggt
      541 gcttgtttaa tccagtatgg agatacttca atactagtta ctgctacttc ttctaaagaa
      601 cctagggaag gtatagattt tttcccatta agtgttgatt atgaagaaag attgtactct
      661 gtaggaaaga taccaggagg ttttataaaa agagagggta ggcctagcga gaaggcaatt
      721 ctaacatcca ggttaataga tagacccata agacccttat ttccagaagg ttatagaaac
      781 gatgtacagg taatagccac agtattgtca gtggaccaag atcattcgcc agaaattgct
      841 gcaatgatag gttcttctgt agccctttct atatcagata ttccttttga tggaccaaca
      901 ggatctgtac ttgttggatt agttgatgga aagtttatta tcaatcctaa cagtgaagaa
      961 agggataagt cagatattca cctgatagta tctggtacca aggatgcaat catgatggta
     1021 gaagctggag cagatgaagt accagaggaa accatattgg aaggaatctt gtttgcccat
     1081 gaggaaatca aaaatatatg tgattttata gaaggtattg ccaatgaggt aggaaaagaa
     1141 aaacaagatt atcaagtatt tatgcctgat aaggaactgg aagaaaaaat cattgaatat
     1201 ggaactgaga aactaattaa agctataaat acagttgaga aacaggaaag agaagataag
     1261 atagacctgg taaaggatga aatcctggag aagtttattg aagaatatcc tgacaatgaa
     1321 aaagatatat tggcagtaat agacacaata ataaaaaagg aagtaaggaa attaataagt
     1381 gaaaagaacc tgagacctga caatagacaa cctgacgaga taagggagat aacatcagag
     1441 gtaggcctac ttcctcgtac acatggttca ggcctattta caagaggtca gacccaagtc
     1501 ttaaccattg ctaccctagg tgcatcttca gatgttcaaa taatagatgg actaggtgag
     1561 gaagagtcaa aacgttatat gcatcattac aatttcccgc cttattcagt aggagaaacc
     1621 aggatgctaa gagggcctgg taggcgtgaa ataggacatg gggctttggc agaaagggct
     1681 ctagagcctg taataccatc agaagaagaa ttcccatata ctattagatt agtatctgaa
     1741 gttttaagct ccaacggatc atcatctcag gccagtgtat gtggaagcac tctttcatta
     1801 ttggatgcag gggtacctat aaaagcttct gtagcaggta tagccatggg attaatcaaa
     1861 gaaggagata aggttacaat cctatcagat atactaggta tggaagatca tctaggagat
     1921 atggacttta aggtggctgg tacaagaaag ggtataacgg ccatacaaat ggatataaaa
     1981 attccaggaa tagataggga aatcttagaa gaagctttag aaaaagctag ggaaggtaga
     2041 ttgtatatcc tagataagat ggaggaaacc atatcagaac ctaggaaaga attatcacct
     2101 tatgcaccaa aaatatttac catgcagata gatccggaaa aaattaggga tgtaattggt
     2161 ccaggtggaa agatgattaa taaaatcatt gatgaaactg gagccaaaat agatatagat
     2221 gatgatggac aaatatttat agctacatct gattcagata gtggtgaaag agctaaagaa
     2281 atgatagaat ccatagtgaa agaagcagaa gtaggagatg tctacttagg taaagttacc
     2341 aaaataacta attttggggc cttcgtggaa attttaaatg gaaaagaggg actgcttcat
     2401 atttcaaaca tagctaggga aagaataaac agggtagaag acgtattaaa agtaggagac
     2461 gatgttctag taaaggtaat aggaatagat agcaagggta taagcctatc tagaaaggct
     2521 ttactaccag atccagataa aaaagaagaa taaagtaata acctccacat ggacctattt
     2581 aggttcatgt tttacttttt tgtataatat ttttcttatc taagaatata cattttacaa
     2641 agagtaaata ggaggatgta aatgagatta ataatcctaa ataaaagaac cttaataatt
     2701 ctagtatcat tggcactagt agttttatta atacttgctt ttacccttgg aagatttagg
     2761 agtaaggaag tatttaaccc agatttgttt tatcagggaa caacagagga aaagctagtt
     2821 gcctttgcct gcaacattga ttggggtggg gaatatcttg aagaaatgct agctatcctt
     2881 gatataaatg aaataaaaat tacctttttt ccaactggca gatgggcaga gaagaatgag
     2941 gctttgttaa aattaatcta tgataaacaa catgagatag gaaatcacgg atataatcat
     3001 ctagattacg ataagttaga cgaagccaat aactatagag agataaaaac agctcatgaa
     3061 attatagaaa aaataattaa tgaccaacct aaatactttg caccaccctc tggagcattt
     3121 aatgaacaca cgatttcagc agccaaacaa ttaggatata aggtaattct ttggagtatt
     3181 gataccatag attggagaga ggatagcaat aaggatgtaa tttatcaaag ggtaatagaa
     3241 aaacttcaca atggcggaat tgtgctgatg caccctacag aagagac
//
LOCUS       DATGSI010000637         1175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_657194, whole genome shotgun sequence.
ACCESSION   DATGSI010000637 DATGSI010000000
VERSION     DATGSI010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657194"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..>1175
                     /locus_tag="VK031_09720"
     CDS             11..>1175
                     /locus_tag="VK031_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HLS54241.1"
                     /translation="MTIGVIIIALFMIVPLVIGFIASAKSEDSSDDYFIQGRGMGSVA
                     AFFTVSATWWSAFAFLGSNASFYMEGPVYLTAFAWNILFGYLYFVVGKRIWYLGKKFD
                     YVTPSDLLGDFYNSEKVRIIVGLIVIIFTIPYLQIQLTGGAYLIEVASGGVIPFWLAA
                     LLFYAVIITYVWVGGMRAVAWTDIVYGALVFFGLLFAGLYISSIVGGPVAMFDQMQAS
                     HPGHLTLPGPEGSSGYSAWISLCFITAIGALMGPQIWLRMYAVKNSKLFNLMPFLLSF
                     VAIAYIGSVFVSWTGVLEMPGVENADQILPLMVMKYLPVAIGALILAGGASAAMSTAN
                     SQIHAVSAVVTKDFYQRYANPLASQSKLIQMGRYFLLIFSILAYFLALFAPGLL"
BASE COUNT          299 a          180 c          266 g          430 t
ORIGIN      
        1 gtgagtaaag ttgactattg gggttattat tattgcttta tttatgattg ttcccttagt
       61 aattggattt atagcgtcgg caaaatcaga ggattccagt gatgattatt ttattcaagg
      121 taggggtatg ggatctgttg ctgccttttt tacagtatca gccacatggt ggagtgcatt
      181 tgcctttctt ggctctaatg cttcgtttta tatggaagga ccagtttatt taacagcttt
      241 tgcgtggaat atattattcg gttatttata ttttgtcgtg ggaaaaagaa tttggtatct
      301 aggaaaaaag tttgattatg taactccttc tgacctgtta ggggattttt ataatagtga
      361 aaaggtacgt attattgtag ggttaatagt tattatattt acgatacctt atctgcaaat
      421 tcaattaaca ggtggcgcct atttaataga ggtggcttct ggaggagtta tccctttttg
      481 gctagctgcg cttctgtttt atgcagttat tataacttat gtttgggtcg gtggaatgcg
      541 agcagtagca tggacagata tcgtatatgg agcacttgtg ttttttggat tgctttttgc
      601 tggtctttat atctccagta tagttggagg accagtagcc atgttcgatc aaatgcaagc
      661 gtcacatccg ggacatctta ccctacctgg acctgaagga tcatctgggt atagtgcatg
      721 gatttcttta tgttttataa ctgcgattgg agctttaatg ggaccccaga tttggttacg
      781 tatgtatgcg gtgaaaaata gtaaactatt taatttaatg ccatttttac tttcctttgt
      841 agccattgca tatattggtt ctgtgtttgt tagttggaca ggtgtattgg agatgccagg
      901 ggtggagaat gcagatcaaa tactacctct aatggttatg aaatatttac cagttgctat
      961 tggtgcttta atattagctg gaggggcctc agcagcaatg tcaacggcta atagtcaaat
     1021 tcacgcagtc tcagcggtag tgacaaaaga cttctaccaa aggtatgcca atccattagc
     1081 atctcagagt aaattaatcc agatgggaag gtatttcctc cttatatttt ctatactagc
     1141 ttatttttta gctctatttg caccaggtct tttag
//
LOCUS       DATGSI010000638         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_564008, whole genome shotgun sequence.
ACCESSION   DATGSI010000638 DATGSI010000000
VERSION     DATGSI010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564008"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VK031_09725"
     CDS             <1..369
                     /locus_tag="VK031_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HLS54242.1"
                     /translation="NPMHGRDENGALASLNSVAKIPYGSVCQDGISNTFSITPQTLGS
                     NYGDRIKNLVGIMDGYFEQDAFHLNVNVLNRDLLMDAMENPAQYPTLTIRVSGYAVHF
                     NRLTREQQLEVINRTFHERV"
     gene            375..1145
                     /gene="pflA"
                     /locus_tag="VK031_09730"
     CDS             375..1145
                     /gene="pflA"
                     /locus_tag="VK031_09730"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004837182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HLS54243.1"
                     /translation="MTKIAEARLHSIETLGLVDGPGIRTVFFLQGCPLRCLYCHNPDS
                     QTMFSKRQISSDEVVEIAKRYKPYYKNTGGGVTFSGGEPLLQAEFIIETAKKLKEEGI
                     TTAIDTTGYGDKRYLEEVLKHVDIVLLDIKHFDNEGYKNVTGKSMGGFYNFLSLLDDF
                     NGTIWIRHVMVPSFTDNKESIINLFNRVKHLAHKINRFEILPYHKMGLEKYERLNRKY
                     PLEGIPEMDREKAQEYQDMLAKMLEQERKSMQLDKKIG"
     gene            1186..1905
                     /locus_tag="VK031_09735"
     CDS             1186..1905
                     /locus_tag="VK031_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLS54244.1"
                     /translation="MLQDIKCNRNCPICEGNSCIHNIPLLSGLNNKEVDKIHAGVTTK
                     AYKKGEHIFRTGDKADKLYIVCAGKIKIYKYLSDGREQILYIYSAGDFVGAFNLLKED
                     EYKYNAQALEDTVISTLTKKKFDEIVIKNPRMTLKILEKAYERIRWAENLVDRLSSGN
                     ADSKVAGLLLYLIDDFGTKTAEGIELNLSINREEMGSYAGISRETMTRKLLQFREFGY
                     IDFEGNKKIIILDKEKLKELL"
     gene            2018..>2748
                     /locus_tag="VK031_09740"
     CDS             2018..>2748
                     /locus_tag="VK031_09740"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HLS54245.1"
                     /translation="MKIKNRLFDIWQSLKESSQRFPITVGMSLLLFILLIYYNENLEN
                     LSNLQQDRFIRFNMIIGLGIGLSLVISLIGERFFPKDKTRKILAHMGGTIGLIFYYKY
                     FLREVRGVTIIRYFGVLILLWILFFYIPRYKVKESYEKYVIKIFANFFVTLIYSLVLL
                     FGIFIILFTVDSLFDVNIKGKYYYYVYLFVSLVFSIALFLSKLPEADERFFDYNYSNS
                     LRVLLTYIIIPLISIYTIILYLYFA"
BASE COUNT          997 a          372 c          527 g          852 t
ORIGIN      
        1 aatccaatgc atggcaggga tgaaaatggt gccctagcct ccttaaattc agttgctaag
       61 ataccttatg gtagtgtatg ccaggacgga atatctaaca ccttctccat aacaccacag
      121 acacttggta gcaactacgg agacaggatt aagaatttgg taggaattat ggacggatac
      181 tttgagcaag atgccttcca tttaaacgta aatgttttaa atagggatct attaatggac
      241 gccatggaaa atccagccca atacccaacc ttaaccataa gggtatctgg atatgcggtc
      301 cacttcaata ggttaacaag agagcaacag ttagaggtta taaacagaac attccacgaa
      361 agagtataga aagggtgact aaaatagcag aagctagact tcattcaata gaaactttag
      421 ggttagtaga cggtccagga attagaacgg tgttctttct acagggttgc cctttaagat
      481 gtctttattg ccacaatcca gacagccaaa ccatgttttc taaaagacag ataagctcag
      541 atgaagtggt ggaaattgct aaaaggtata aaccttacta taaaaatact ggcggaggtg
      601 tcaccttttc aggaggtgaa cctctgcttc aggcagaatt tataattgaa actgcaaaaa
      661 aactgaagga agaaggaatt accacagcta tagatacaac tggatatggt gacaaaagat
      721 atctagaaga ggttttaaaa catgtagata tagttttatt agatattaaa cattttgata
      781 atgaaggtta taaaaatgtt actggaaaaa gtatgggggg tttctataat tttctatccc
      841 ttttagatga ctttaatggt acaatatgga taagacatgt tatggttccc tcttttacag
      901 ataataagga aagtattata aacttattta atagagtcaa acacttggcc cataaaataa
      961 atcgttttga aatcctgccc taccataaga tggggttaga aaaatatgaa agactaaata
     1021 gaaagtatcc tcttgaaggt attccagaaa tggacagaga aaaagcacaa gaataccagg
     1081 atatgttggc taagatgtta gagcaggaaa gaaaaagtat gcagctggac aaaaaaatcg
     1141 gatagggaat ctagatatgt ctataacata taagcaggtg attaaatgtt acaagacata
     1201 aaatgtaata ggaattgccc catatgtgaa ggtaattcct gcattcacaa tattccccta
     1261 ttatcaggtt taaataataa agaagtagat aaaattcatg caggtgttac tacaaaggcc
     1321 tataaaaaag gagaacacat ttttagaact ggagataagg cagataaatt atatatagtt
     1381 tgtgcaggca aaataaagat atataagtat ttgtctgatg gtagagaaca aattctttat
     1441 atatattctg ccggagactt tgtaggtgca tttaacttgc taaaggagga tgagtataag
     1501 tataatgctc aggctcttga agacacagtt attagtacct taacaaagaa aaagtttgat
     1561 gagattgtaa taaaaaatcc tagaatgact ttaaaaatat tggagaaggc ctatgaaaga
     1621 atcagatggg ctgaaaatct ggtagataga ttatcttcag gaaatgcaga ttctaaggta
     1681 gcaggtctac ttctatattt aatagatgat tttggcacga aaacggcaga gggaatagaa
     1741 ttaaaccttt ctattaatag ggaagaaatg ggaagttatg caggtatatc tagagagacc
     1801 atgaccagaa agctattaca atttagagaa tttggctata ttgattttga aggtaacaaa
     1861 aagattatta tactagataa ggaaaagctt aaggagctat tataggttaa cctataatag
     1921 ctcctttttt tagagatatt aactatatat tactgcaata tatgggtaga tttgatatga
     1981 taagatcaag taaataggaa aagttggaga tgatgagatg aagattaaaa ataggttatt
     2041 tgatatctgg cagtccctta aggaaagtag ccaaaggttt ccaattaccg ttggcatgag
     2101 tctcttatta tttattttat taatttatta taatgaaaat cttgaaaacc tatccaacct
     2161 acaacaagat agatttataa ggtttaatat gattataggg ctgggaatag gtttatcact
     2221 ggttattagc ctaataggtg aaagattttt tcccaaggat aaaacaagga aaattttagc
     2281 ccatatgggt ggcactatag gattaatttt ctattataag tatttcctaa gagaggtaag
     2341 aggagtaact attattaggt attttggagt tttaatactt ttatggattt tattttttta
     2401 tattcctaga tataaggtca aggaaagtta tgaaaagtat gtaatcaaaa tttttgccaa
     2461 cttttttgta accttgattt attccctggt tctactattt ggaatattta ttatcctatt
     2521 taccgttgat agtctatttg atgtaaatat taaggggaaa tattattact atgtatattt
     2581 atttgtatcc ctagtattta gtatagcctt attcctatcc aaactacctg aggcagatga
     2641 acgtttcttt gactataact actccaattc cttaagggtt ttgttaacct atataataat
     2701 acctctaatt agcatttata ccataatcct ttatctatat tttgcaaa
//
LOCUS       DATGSI010000639         5140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_564153, whole genome shotgun sequence.
ACCESSION   DATGSI010000639 DATGSI010000000
VERSION     DATGSI010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564153"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VK031_09745"
     CDS             <1..614
                     /locus_tag="VK031_09745"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HLS54246.1"
                     /translation="GALMYDFGGPIGEIMISTMGHPEVFTIPRVLPEAKNITIKLGFY
                     PSESFEAMRDLIEAGLASEEPLLVNGKEVVPRDFLVSQMINDIKHDKAERKLGEPVTG
                     SVVRVTGIKDGKRVEYTNRSFGRMGPATGYPLAIGAEMLAQGKIEKKGMMVPEECIDP
                     IPFLTEFATRSAKAGHLSIGRTDIKTEEVSDPQGLLSKHGIKF"
     gene            855..1826
                     /gene="pta"
                     /locus_tag="VK031_09750"
     CDS             855..1826
                     /gene="pta"
                     /locus_tag="VK031_09750"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HLS54247.1"
                     /translation="MSLIENLKKEIRDKQASIVFPEGNDSRVLRAAIRLKEDGDLNPI
                     VLGNVEEIQNLASKENVDLGKLEIIDPENYEDFDAMVDAFVERRAGKATAEEAREILK
                     QPNYFGTMYIYMDKADGMVSGAIHTTGDTVRPALQIIKTKPGIRRVSGAFIMLREDEM
                     LFMADCAINTDLDEDDLAELAVVSENTARQFGMDPKVAMLSFSTKGSAKHDRVDMVRN
                     ATEKAKELNPDMVIDGELQFDTAYVESVGAQKAPDSPIKGDANVFIFPNLEAGNIGYK
                     MAQRLGGYEAVGPILQGLNKPVNDLSRGTSEDEVYALAIITAAQAVL"
     gene            complement(1873..3126)
                     /locus_tag="VK031_09755"
     CDS             complement(1873..3126)
                     /locus_tag="VK031_09755"
                     /inference="COORDINATES: protein motif:HMM:NF019244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HLS54248.1"
                     /translation="MKNESTNNFLNKYKIELITLPYLGFFLITGLFMQPFSQLLEGLI
                     NIFTAPGVLLTDYILIAGLGPALVNASLVGFLGYFLLIFSGATLNGLSVAAIYTLFGF
                     GLFGKTIWSILPIMLGVFIYSRLSGQSMATNVYAALFGTALAPFVTQTAFGFRLGLIP
                     GILIGIIAGFLVAPIAKHAFTFHQGYNLYNIGFASGLVGLLILSILRAFNLDSQGTIL
                     WSTEYDGLLRILVIGMFAFMLGLGAFLSRTELLDYKKILKHAGRPAVDFTEEAKLGHT
                     LINMALVGLIGVVYIELVNGNYNGPSLGGLYTMVGFAAFGKHPFNVTPIMLGVWLATA
                     FSIYSPADPGPMVAALFGTALAPVAGDFGPKIGILAGIIHLFIVTQIGVVHGGLNLYN
                     NGFSAGFVAAIFLAFKKDFSLKKRY"
     gene            3312..4853
                     /locus_tag="VK031_09760"
     CDS             3312..4853
                     /locus_tag="VK031_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HLS54249.1"
                     /translation="MEESNTFLNRAKKKIKNIKNKKRFFAVISTALILLSIGTGFGIN
                     YYKKLKNPQDLFIPEIENNEDPTDIDISEQFDRNIVNILMVGFDRDEKRSKEESAFRT
                     DTNVVASINLEKKTVDLLSVPRDSYVPIANMGGKKDKFNSSFVYGYIYGNGPDRDENG
                     YKYLLETTRELLGGAPLHYYVAVDMDVVVEIVEAIGGVELDIPEDVVRGNKVRIKKGY
                     QKLDGRSLLFYARNRNLPGGDIDRVKNQQRILMAIFDTLKKSNKFTALPKIYRSMMDK
                     IDTNLNFNQLTALALFGMELDKDNINMHMLKGNYGHFNGISYWIVDQEARVELIEKLY
                     GLRIEPDPQDPIRETAQSFKANITRTNFEVGQQAKITARGATNVTAYKEFRPSEMRFS
                     SSNSNVVVVDKNGIVKAVGPGEAIITVSIDGLTNQLYLVVNKPAAHKDKKDEEKDHKK
                     DPDKDKTKVEDEKDKEEPDEGQNGNEGQNKGEEGAGEDKMENDEKGNGSTKPDEDKDK
                     RNKEE"
     gene            5002..5127
                     /locus_tag="VK031_09765"
     CDS             5002..5127
                     /locus_tag="VK031_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54250.1"
                     /translation="MCKYRLKIISEKQSRTIVGNAGFPPLTGTCIRMYMKSAARI"
BASE COUNT         1905 a          734 c         1099 g         1402 t
ORIGIN      
        1 agggagccct tatgtatgat tttggtgggc caattggaga aattatgatt tccacaatgg
       61 ggcatcctga agtatttaca ataccaagag tattgccaga agctaaaaat ataaccataa
      121 aattaggctt ttatccaagt gaatcctttg aagcaatgag agacttaatt gaagcaggtc
      181 tggcttctga ggagccattg cttgttaatg gaaaagaagt agtgcctaga gactttttag
      241 taagtcagat gataaatgac ataaagcatg ataaagcaga gaggaaatta ggtgagccgg
      301 taactggctc tgtggtaagg gttacaggta taaaggatgg aaaaagggta gaatatacaa
      361 atagatcttt tggtagaatg ggaccagcta ctggttatcc tttagcaata ggggcagaaa
      421 tgttggcaca gggtaagata gagaaaaaag gaatgatggt accagaagag tgtatagatc
      481 caattccctt tttaacagag tttgctacac gttctgcaaa ggctggacat ttgtctatag
      541 ggagaacaga tattaaaact gaagaggtat ctgatccaca aggcctgtta tccaaacatg
      601 gtattaagtt ctagattaaa ttaaaaatat taaatattat agctagaaga tgacattgac
      661 cataactatg attgatgtca tcttttgttt catagacata tgccttgtac aagactatca
      721 catatattat aattgggctt ggaggtcctt attgataagt atagaaatca aaagtttgtc
      781 aataatgtaa ctttatttta taatggtatg tagtctatta atatatgaaa atgataagta
      841 taaaggagga caaaatgtca ctgattgaaa acttaaagaa agaaataaga gacaagcagg
      901 catcaatagt atttccagaa ggaaatgaca gcagggtttt acgggctgcc ataagattaa
      961 aagaggatgg agacctaaat cctatagtat taggaaatgt ggaagaaata caaaatttag
     1021 caagtaaaga aaatgtagat ttaggaaagc ttgagattat agacccggaa aactatgaag
     1081 actttgatgc tatggtggat gcctttgtgg aaagaagggc aggaaaagca acagctgagg
     1141 aagcaagaga aatcttgaaa cagccaaatt attttggaac catgtacatt tacatggata
     1201 aggcagatgg catggtatca ggagctatac ataccactgg tgatactgta aggccagctt
     1261 tacagattat taaaactaag ccaggaatta ggagagtaag cggtgctttt ataatgctta
     1321 gagaagatga gatgttattt atggcagact gtgctataaa tacagattta gatgaagatg
     1381 atctggcaga actggctgta gtgtcagaaa atacagctag acaatttggt atggatccaa
     1441 aggttgccat gttatctttc tctactaaag gttcagccaa acatgataga gtagatatgg
     1501 tgagaaatgc aacagaaaag gccaaagaat taaatccaga tatggtaata gatggagaat
     1561 tacaatttga tacagcctat gtagagtcag taggggcaca aaaggcacct gattctccaa
     1621 ttaagggaga tgccaatgta tttatttttc caaacctgga ggcaggaaat attggttaca
     1681 agatggctca aagattaggt ggatatgagg cagtaggtcc aattttacag gggctaaata
     1741 agcctgtaaa tgatttatct aggggaactt cagaagatga ggtatacgct ttagccataa
     1801 ttactgcggc tcaggctgta ttataaataa aaagggtcca actaagattt cttaagttgg
     1861 acccttttaa ttctagtatc ttttctttaa ggaaaaatct tttttaaagg ctagaaaaat
     1921 tgcggcaaca aaacctgccg agaagccatt attgtataga tttaagcctc catgcacaac
     1981 tccaatttgt gtaactataa ataaatgtat tatgcctgct aagataccaa tcttaggtcc
     2041 aaaatcacct gccacaggag ctaaggcagt accgaaaagg gcagccacca tagggccggg
     2101 atcagctgga ctatatatgg aaaaggctgt agccaaccat actccaagca taataggtgt
     2161 tacattgaat gggtgttttc caaatgcagc aaagccaacc attgtatata atccacctaa
     2221 gctagggccg ttataatttc cattaaccaa ttctatataa acaactccaa ttaaaccaac
     2281 cagggccata ttaataagag tatggcctag ttttgcttct tctgtaaaat ctactgctgg
     2341 ccgaccagca tgtttaagaa ttttcttata atctaaaagc tcagtcctgc ttagaaatgc
     2401 tcccagtcct aacataaagg caaacatacc tataaccaag attcgtaaaa gaccatcata
     2461 ttctgtagac cataggattg taccttggct atctaggtta aaagccctaa gtatgcttaa
     2521 gattaatagg cctactaaac ctgaagcaaa gcctatatta tataaattat atccttgatg
     2581 aaaggtaaag gcatgtttag ctataggagc tactaaaaag cctgctatta tacctattaa
     2641 aataccagga ataagaccta gtctgaagcc aaaggctgtc tgggtaacaa agggtgccaa
     2701 agcagtacca aacaaggctg cataaacatt ggttgccata ctttgtccac ttagtctact
     2761 gtatataaat acacctagca ttataggtag gatagaccat atggtttttc caaaaagacc
     2821 gaatccaaat agggtataaa tcgcagctac agatagacca ttgagggttg ctccactaaa
     2881 tattagaaga aagtatccta aaaatccaac tagtgaagca ttaactaggg ccggacctag
     2941 gccagctatt aagatatagt cggttagaag aacacctggt gcagtaaaga tatttattaa
     3001 tccttccagt agctgactaa agggctgcat aaagaggcca gttattaaaa agaatcctaa
     3061 atagggtagg gtaataagtt caattttgta tttgtttaaa aaattattag tagattcatt
     3121 tttcatgtca agtctcctat atttttaagt tagattataa caaaatatag ggataaaata
     3181 aagcaaataa ttattatcta agatatttaa aatgtatatt taaaggatat ggactatatg
     3241 atacaatagg tataattaaa taaaggggct tatagttatt tataaatagt ttttacaagg
     3301 aggaaattcc catggaggag agtaatacct ttttaaatag ggctaaaaag aaaattaaaa
     3361 atataaagaa taaaaagcgt ttttttgcag ttattagtac agcactaata ctattaagca
     3421 taggaactgg attcggaatc aattattaca aaaagctgaa aaatccccag gatctattta
     3481 taccagaaat agaaaacaat gaggatccaa cagatataga tattagtgaa cagtttgata
     3541 ggaatattgt aaatatctta atggtaggct ttgatagaga tgaaaaaagg agtaaagaag
     3601 agtctgcttt taggactgat accaatgtgg tggcaagtat taacttagag aagaagactg
     3661 ttgatttatt gtctgtacct agagatagct atgtacctat tgcaaatatg ggtgggaaaa
     3721 aggacaaatt taactcatcc tttgtatatg gatatattta cggaaacgga cctgataggg
     3781 acgaaaatgg ttacaaatac ttattagaaa ctaccagaga attattagga ggggcacctc
     3841 ttcactacta tgtggcagta gatatggatg tggtagttga gatagtagaa gccataggtg
     3901 gagttgaact agatattcca gaggatgtag taagaggaaa taaagttagg attaaaaaag
     3961 gctatcaaaa actagatggt agaagcttgt tattttatgc aagaaataga aatttgcctg
     4021 gaggagacat agatagggta aaaaatcagc aaagaatttt aatggccatt ttcgataccc
     4081 ttaaaaagtc aaataagttt acagccttgc ctaaaatata tagaagcatg atggataaga
     4141 tagacacaaa tctaaatttc aatcaattga cagccctagc cctatttggt atggagctgg
     4201 ataaggataa tataaatatg catatgttaa agggaaatta tggacatttt aacggcattt
     4261 cctattggat tgtagatcaa gaagcccggg tagagttaat tgaaaaacta tatggactcc
     4321 gtatagagcc tgatcctcag gatcctatta gagagacagc ccaatccttt aaggctaata
     4381 ttacaaggac caattttgag gtaggccaac aagctaaaat tactgctagg ggggctacaa
     4441 atgttactgc ctataaggaa tttaggccta gtgaaatgag attttcatct tctaactcga
     4501 atgtagttgt ggtagataaa aatgggatag taaaggcagt aggacctgga gaggccatca
     4561 ttacggtaag catagatggc ttaacgaacc aattatattt ggtagtaaat aaaccagcag
     4621 cccataagga taagaaggat gaagaaaaag atcataagaa ggatccagac aaggacaaaa
     4681 caaaagttga agatgaaaag gataaagaag aacctgatga ggggcagaat ggtaatgagg
     4741 gccaaaacaa gggtgaagaa ggtgccggtg aggataaaat ggagaatgat gaaaaaggaa
     4801 acggctcaac taaacctgat gaggataagg ataaaagaaa taaagaagaa taaaaaggaa
     4861 aatgcttatc taaaatttcc attgtaaatg ggaaagattt attgtaaaat atatatatct
     4921 aatttaatat ataaattcta tgaagagaaa gagtaggctt attatttgtt ccccagagag
     4981 ttggcgatta ggtgaaagcc aatgtgcaaa tataggttga aaatcatctc tgagaagcag
     5041 agccgaacta tagtaggtaa tgcaggtttt ccaccgttga caggaacatg tattaggatg
     5101 tacatgaaaa gtgctgcaag gatttagcaa atttttgtag
//
LOCUS       DATGSI010000640         1258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_323671, whole genome shotgun sequence.
ACCESSION   DATGSI010000640 DATGSI010000000
VERSION     DATGSI010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323671"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VK031_09770"
     CDS             complement(<1..1145)
                     /locus_tag="VK031_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HLS54251.1"
                     /translation="MRNYLQRIGRSLMLPVAVLPAAAILMGIGYWIDPSGWGGGNAVA
                     AFLISAGSAIIDNMPILFAIGVALGMSKDQDGSAALSGLVAFLVVTTLLSTDTVAMLM
                     GIDAAEVNPAFGSIGNQFVGILSGVVAAAMYNRFSHVQLPDALAFFSGRRLAPIVTSV
                     AMIGVSVILFFVWPAIYTGLVNFGTGIVGLGAIGAGIYGFFNRLLIPVGLHHALNSVF
                     WFDVAGIDDITKFWASEGTKGVTGMYQAGFFPIMMFGLLGAAFAIYQTARPEKKKQIA
                     SLMLAAGFASFFTGVTEPLEFSFMFVAPLLYFFHAVLTGISLTIAATFQWIAGFGFSA
                     GFVDWFLSLRMPLANKPWMLIVQGLVFAAIYYVVFRFAIVKFNLTTPG"
BASE COUNT          425 a          301 c          194 g          338 t
ORIGIN      
        1 cctggtgtag ttaaattaaa tttaacaatt gcaaacctaa atactacata ataaattgct
       61 gcaaagacta gcccttgtac tattaacatc caaggcttat tggccagagg catccttaaa
      121 cttaagaacc aatctacaaa cccggcacta aatccaaatc ctgcaatcca ttggaaagtt
      181 gcagctatgg ttagagaaat acctgttagc actgcatgga agaagtataa taatggtgcc
      241 acaaacataa atgagaactc taaaggttca gttacccctg tgaagaagga tgcaaaacct
      301 gctgctaaca ttaatgaagc aatttgcttc ttcttctctg gcctggctgt ttgatagatt
      361 gcaaaggctg cacctagtag gccaaacatc ataatcggga agaatcctgc ctgatacatg
      421 ccagtaactc cctttgttcc ttcactggcc cagaacttag ttatatcatc aattcctgcc
      481 acatcaaacc agaatacaga gtttaaagca tggtgcaaac ctactggtat taataatcta
      541 ttaaagaatc cataaattcc tgcacctatt gcacctaaac caacaatccc agtaccaaag
      601 tttactagcc ctgtatagat agcaggccat acaaagaata atataacaga aactcctatc
      661 atggctactg aagtaactat tggtgccaat cttctaccac tgaagaaggc caatgcgtct
      721 ggaagctgta catgactaaa cctattatac atggccgccg ccaccacacc agaaagtatt
      781 cctacaaatt ggttgccaat actaccaaaa gctggattta cctctgctgc atcaataccc
      841 attaacatgg ctactgtatc agtagacagc aaggttgtaa ctactaaaaa tgctacaagt
      901 ccacttaatg ctgcagaccc atcttgatcc tttgacatac ctaatgctac ccctatagca
      961 aatagtatag gcatattatc aatgattgca gaaccagcac ttattaagaa agcagctaca
     1021 gcattacctc caccccaacc agatggatca atccagtaac caattcccat taatattgct
     1081 gctgccggca atactgccac gggaagcatt aatgaacgtc caattctctg taaataattt
     1141 ctcataatta ctgtcctcct ttaattttta taatcaatta taaatgcgac ttaaaactag
     1201 cctatccccc tttccttaaa agttaaaagg cataaaccta cccatacttt tggtatga
//
LOCUS       DATGSI010000641         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_564306, whole genome shotgun sequence.
ACCESSION   DATGSI010000641 DATGSI010000000
VERSION     DATGSI010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564306"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VK031_09775"
     CDS             complement(<1..513)
                     /locus_tag="VK031_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54252.1"
                     /translation="MKPVQVLENLLASVRARKVKSGFIYLVSEELLPEVYAGIKAKEY
                     KLKGGNIYVLYGKDGMEVYVPVEDGAPDDSNLISAGGGSEEIVEVLDNIATEVGRENS
                     GDPTYESTFEDLLSGMLGIVIDLESQAPLATEQYVTENFAPNVHTHTIGQVTGLQGEL
                     DGKANSTHTHV"
     gene            complement(518..1108)
                     /locus_tag="VK031_09780"
     CDS             complement(518..1108)
                     /locus_tag="VK031_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54253.1"
                     /translation="MKQVGYIVTLCLTLLLLNSCGLLRKQSKVEFKEEIVQKAEKEVV
                     EKEEEVVEQKDVKQKEEKIVKDSSKESKIEFKGDLEVIENVSLKEGLNVLETKSGGTV
                     FVVKKDDDISLVINRPDITVFNKDFDILEEFKKGLTDSSNTRTDTKSKEGRESRSESK
                     ALLKNEKKYTPSLKVILIMIGLITGLGILTYRKFKN"
     gene            complement(1228..>1597)
                     /locus_tag="VK031_09785"
     CDS             complement(1228..>1597)
                     /locus_tag="VK031_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54254.1"
                     /translation="NINDVESKREIKVDINRDFIDRKIEGGVGGVERLFHLALKSPLT
                     LLLVLSILANIYLGNKLIKTNEEMRIQIIEEVRRQVPVEVQKETSEQLNSVSEKVDTI
                     YNQSREFYRTIKEIGGIAHD"
BASE COUNT          398 a          364 c          222 g          613 t
ORIGIN      
        1 tacatgggta tgtgtagagt tagctttacc atccagttcc ccctgaagtc cagtcacttg
       61 ccctattgta tgtgtgtgga cattaggtgc aaagttttct gttacatact gttctgtagc
      121 aaggggggct tgggactcta aatctattac aatacctaac ataccagaga gtaagtcctc
      181 aaaagtagat tcatatgtgg ggtctcctga attttccctt cctacctctg ttgctatatt
      241 atcaaggact tctacgattt cttctgaacc tccgcctgct gaaattaggt tactatcatc
      301 gggtgcgcca tcttctacag gtacgtaaac ctccatacca tccttaccgt atagtacgta
      361 tatgtttccc ccttttaatt tatattcttt agccttaatt cctgcgtaca cctcaggtag
      421 taattcttca ctgactaggt atataaatcc tgactttact ttccttgctc ttacactcgc
      481 taataagttc tctaatactt gtactggttt cattccttta atttttaaac tttctatatg
      541 ttaatattcc cagacctgtt attaatccta tcataataag aataaccttc aagctaggtg
      601 tgtatttctt ctcgtttttg agtagagctt tgctctctga gcgactttct ctaccttcct
      661 tactctttgt atcagtccga gtattggagc tgtctgtcag tcctttttta aactcttcta
      721 atatgtcgaa gtctttgttg aatactgtga tgtctggtct atttataaca agggatatgt
      781 catcatcctt cttaactaca aacactgttc ctccagactt agtttctaaa acattcaacc
      841 cttctttcag ggacacattc tcaataacct ctaagtctcc tttaaactca atcttacttt
      901 cctttgaaga gtctttaact attttctctt ctttctgctt aacgtccttt tgttctacta
      961 cttcctcttc cttttcaact acttccttct cagccttctg gactatctct tctttaaact
     1021 caaccttact ttgttttcgt aataatccgc aactgttcaa tagaagcagt gtcagacata
     1081 aggtcactat atatcctact tgtttcatct agttttatat ttaaatctcg ttttaactcg
     1141 cataaagagt ggaggttgac ttcttccatt tgtttagggg aagacaatgt gtaaacaaac
     1201 aatagcgcca ttataaatac tatcttacta gtcatgcgca atacctccta tctctttgat
     1261 agttctgtaa aactcccgac tttgattgta tatagtatca actttttcac taacactatt
     1321 cagctgttcg gaagtttcct tttgaacttc tactggtact tgtctacgta cctcttctat
     1381 aatctgaatc ctcatctctt cattagtctt aattaatttg ttccccaaat aaatgttagc
     1441 tagaatactt agtactaaca ataatgttaa agggctttta agagcgaggt ggaagagcct
     1501 ttcaactcct cctacaccgc cctctatttt tctatctatg aagtctctat taatatctac
     1561 ctttatttcc cttttgcttt ccacatcatt tatgttt
//
LOCUS       DATGSI010000642         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_274899, whole genome shotgun sequence.
ACCESSION   DATGSI010000642 DATGSI010000000
VERSION     DATGSI010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274899"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(718..1677)
                     /locus_tag="VK031_09790"
     CDS             complement(718..1677)
                     /locus_tag="VK031_09790"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLS54255.1"
                     /translation="MKRLFVYIFLIVLIITACSKQEEKVQFSHEEYNLDLDEGTIYGT
                     LTLPEGKGPFPVALIIAGSGPTDRDGNNPLAGVNNSLKMLAEALAENSIASVRYDKRG
                     IAESKDLMVREEDLSFEDYVEDAAAWMDKLNRDERFKGHYIIGHSEGALIGVVAANKS
                     DVEGYISIAGVGRPADDLLKEQLKNQSEYIYKKSLPVIEELKKGNLVPDIDKELEIIF
                     RPSVQPYLISWFKYDPREEIGKLKEPILILQGDKDLQVSVDDAESLFKSISTRNKMEI
                     IKGMNHVLKNVSGDEKENIKSYSNPDLPIDQDLVTEIVKFIGR"
     gene            1910..2374
                     /locus_tag="VK031_09795"
     CDS             1910..2374
                     /locus_tag="VK031_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HLS54256.1"
                     /translation="MKIYVDADGCPVVDVAISIAKIYGLDIIIVKNYAHQIEDDYAEI
                     VSVDISSDSADLYIVNQIKRNDIVITQDYGLAALSLSKGAIAINQNGIIYTNKNMDQL
                     LGQRHIHRELRRQGHFVGKARKRNSKQDKSFEIRLKDLIKSKKEEMYGSDHR"
BASE COUNT          748 a          472 c          350 g          900 t
ORIGIN      
        1 ttgtatattt ttcagtaaca agactatcca aaacatcata ttcataaata tccatattat
       61 ctaatcactc cctttttgtg aacaatagct tagtcccact gaatttaaat ttactatatt
      121 taatacaacg gtctttatta acaccctgtg tggcttgtcc atgttgatgg caaacaaggt
      181 ctttaacaac ctatgacaag gcttttgttc atctaagggg aaagccatgt aaatttaaaa
      241 tttcctatta cctatattga taagttcact tccttacagc ttccattatg tcccttatat
      301 taatttaaat cctccttcca taattattct tgtatctgta aaatccatat aaaagcttta
      361 tccaaggtct aaactctctt tgatttagtc aataatataa gttcctttat tttattatat
      421 catgttcaga aaaaatcggt tctagctaat catcagaggt tctccacgct ttatgttgaa
      481 tgcaggataa atttatactt catccaaagt atatttttga atttattgta tattatattc
      541 cctttcatct tctagtagat tccatattta tgcctaggtc cttattattt attgtataat
      601 tacattatca ggagcacctc ttgtttaact tctgttattt atatgttatt atatgaaaat
      661 gtttttgttt actttaacaa gattatctta cactagtcta ttacctgatt gaaagaatca
      721 tcttccaata aattttacaa tttctgtcac caagtcctga tctataggca ggtctggatt
      781 actataggat tttatatttt ccttttcatc tccggacaca tttttaagca catggttcat
      841 cccttttatt atttccatct tatttctagt gcttatagat ttaaaaagac tctctgcatc
      901 atctactgac acctgcaaat ccttatctcc ctgcagaatt aaaataggct ctttcagttt
      961 cccaatctct tcccttggat catatttaaa ccaggatata agatagggtt gcacacttgg
     1021 cctaaagatt atttccaatt ccttatctat atctgggact aaattaccct tttttaactc
     1081 ttctattaca ggtaggcttt ttttgtaaat atattcagat tggtttttta actgttcttt
     1141 cagtaagtca tctgcaggcc taccaacccc tgctatggat atatagcctt ctacatctga
     1201 tttgtttgca gcaaccaccc ctattagggc cccttcgcta tgccctatta tataatggcc
     1261 tttaaacctt tcatccctat ttaacttatc catccatgca gccgcatcct ccacatagtc
     1321 ttcaaagctt aaatcttctt cccttaccat taggtcctta ctttcagcaa ttcctctctt
     1381 atcatatctt acgctggcaa tagaattttc cgccagggcc tcagccaaca ttttaaggct
     1441 attgtttact cctgctaagg gattatttcc atccctatct gttggtcctg aacctgcaat
     1501 aattaaagcc acaggaaagg gaccctttcc ttctggcagg gttaaggtac catagattgt
     1561 tccctcatca agatctagat tgtattcttc atgggaaaac tggacctttt cttcctgctt
     1621 tgagcaagct gttattatca aaactattag aaatatatag acaaataatc ttttcattat
     1681 ctacactcct tacattacac attattaatg atatggctac ttactttata ttctatataa
     1741 gtctatctta tacattaatt tatatggtaa tcccacctat ctagtcccct tctttaaggt
     1801 taccattttt gtatcttaac tacattatac tattctatcc aattcctgtt ggcatattat
     1861 gttataataa attaacagat aatacatttt tataaataag gatgagaata tgaaaatata
     1921 tgtggatgct gatggctgcc ctgtggtaga tgtggctatt tctatagcca aaatatatgg
     1981 attagatatt ataattgtaa aaaattatgc ccatcagatt gaagatgact atgcagagat
     2041 tgtatctgtt gacatatcct ctgacagtgc agacctttat attgtaaatc agataaaaag
     2101 gaatgatata gtgattaccc aggattatgg tctggcagcc ttatctttat ctaaaggagc
     2161 aatagccatt aatcaaaatg gaatcattta tactaataaa aatatggacc aacttttagg
     2221 tcagcgccat atccataggg aattgagaag acaaggccac tttgttggca aagcaaggaa
     2281 acgtaatagt aaacaggata aaagttttga aatcaggctc aaagatctta ttaaatctaa
     2341 aaaggaggaa atgtatggct cggatcatcg gtagacaaat tatgcttagg gaatatagga
     2401 aggaggatct gccttctatc agaaggtggg ttaatgatag tgaaataaca gataacctgt
     2461 ctgatatttt
//
LOCUS       DATGSI010000643         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_90506, whole genome shotgun sequence.
ACCESSION   DATGSI010000643 DATGSI010000000
VERSION     DATGSI010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90506"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..591
                     /locus_tag="VK031_09800"
     CDS             238..591
                     /locus_tag="VK031_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HLS54257.1"
                     /translation="MAINYKNNYGKVSISETVIANIAASSAMESYGVVGLASRSTKDG
                     IYKLLGLENMNRGVEVSRNINGTVTINISLILNYGVRIAVVCQNIIENVKYNIEQSLN
                     VGVDSVNILVQGVRS"
     gene            599..>1526
                     /locus_tag="VK031_09805"
     CDS             599..>1526
                     /locus_tag="VK031_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HLS54258.1"
                     /translation="MKKIDSSKFKSLIISAFELLNEKKDIVDSLNVFPVPDGDTGTNM
                     TMTMKSGVEKVKSVEGSSCHDIAKALSQGTLMGARGNSGVILSQLCRGMAKAIKGKDF
                     ISSSEINEIFQTANKTAYKAVMKPTEGTILTVSRLMAEKAQEIFEENLDIDKYLLQIV
                     SAGQKALLDTPNLLPVLKEAKVVDSGGQGLMFLLEGALKALNSDIDIETDLSELEIED
                     VKSKDYNINLDIAIDENESDKLKSALTEISSSLNFDFDNGLAQTSFDIDDLNSLYRLL
                     EEFDGLINLNIENTNPDSLANIKINKKPSKKYG"
BASE COUNT          588 a          210 c          278 g          450 t
ORIGIN      
        1 cttaagagat cattccctga aacagctgac caactatacc aagaagcaga aaaagctgct
       61 aacgataggt atcaaacata caagagatta gctggtaaat aagataaatt ttaatataaa
      121 gataaaagga tgagcttgct catccttttt ttatgatata aatgaattat aaattaatta
      181 tactagagta tgatttcatg gtataatgtt tatatatgaa ttaaaatgga ggactttatg
      241 gcaattaatt ataaaaataa ttacggaaaa gtaagcattt cagaaacagt aattgcaaat
      301 attgctgcat cttctgctat ggagtcttac ggagttgtag gacttgcaag caggagtact
      361 aaagatggaa tatataaact tttaggcctt gaaaatatga ataggggcgt ggaagtttct
      421 agaaatataa atggaacagt taccattaat atatctctaa ttttaaatta tggggtaagg
      481 atagctgttg tatgtcaaaa tataattgaa aatgtgaagt ataatataga acaatcctta
      541 aatgttggcg tagatagtgt caacatatta gttcagggag taagaagtta ggtggattat
      601 gaaaaaaatc gatagttcta agttcaaaag cttaattatc agtgcttttg aactattaaa
      661 tgaaaagaaa gatatagttg atagtctaaa tgtatttcct gtacctgacg gggatactgg
      721 taccaatatg accatgacta tgaaatcagg tgtagaaaaa gttaagtcag ttgaaggctc
      781 atcttgccat gatatagcca aggctttaag ccaaggaacc ctaatgggag ctcgtggtaa
      841 ctcaggagtt atcctatccc agctgtgtag gggcatggca aaggcaatta agggcaaaga
      901 ttttatatca agtagtgaaa taaatgaaat atttcaaact gctaataaaa cagcctataa
      961 ggcagtaatg aaaccaactg aaggaactat acttacagta tcaagactta tggctgaaaa
     1021 agcccaagaa atttttgaag aaaatcttga tattgataaa tacctactac aaatagtatc
     1081 agctggacaa aaggccctac ttgatacacc aaaccttctt ccagtactaa aagaagccaa
     1141 ggttgtcgac tcaggtggac aaggattgat gtttttactt gaaggagctc taaaggccct
     1201 aaattctgat attgatatag aaactgacct ttcagaactt gaaatcgaag atgttaaatc
     1261 aaaagattac aatataaatc ttgatatagc aattgatgaa aatgaaagtg ataagctaaa
     1321 atcagccctt acagaaattt cctcatcttt aaactttgat tttgataatg gcctagctca
     1381 aacttctttt gatatcgatg atttaaacag tctttataga cttttagaag aatttgatgg
     1441 ccttattaac ttaaatatag aaaatacaaa ccctgatagc ctagctaata taaaaataaa
     1501 taaaaaacca tctaaaaaat atggtt
//
LOCUS       DATGSI010000644         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_140127, whole genome shotgun sequence.
ACCESSION   DATGSI010000644 DATGSI010000000
VERSION     DATGSI010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140127"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1087
                     /locus_tag="VK031_09810"
     CDS             155..1087
                     /locus_tag="VK031_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLS54259.1"
                     /translation="MILTVALDPVLENRYYVENLYPKTINKADKVLYKISGKGIICTR
                     ILKSLNMDVFSTGFLGGLKGKYIFEELKRQNIYNDFVFIKDETRGNLLLYQKEELIST
                     IAEDGPRITREEIGTFYELFNKLIDRFDLICALGDLPIGVPEEIYFDLIDIAKKRDKR
                     FVLDAKGTELIFGLEAAPFMAIVDREDLEQISRLKLDFENEIIKVGISLLEMGIEAVI
                     IDLDGSGTIVLNHKQGYRLSVPGMENMKVKKDQGYLAAGYVFGIDKGYKFETTMKIGQ
                     GFRLAYAMGDDRDLIDMGHIKKIMNEIDISSIYY"
     gene            1098..1646
                     /locus_tag="VK031_09815"
     CDS             1098..1646
                     /locus_tag="VK031_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HLS54260.1"
                     /translation="MEKFTLDREKTVLLVIDIQERLVPVMKYKDQVIDNTKILLHAAR
                     EMDFPVVVTEQYPKGLGSTVSEIKEDLQDAKIFAKNSFTAYIDEVKESLEDIGRKKII
                     ITGMETHICVFQTTRDLIKAGYEVHIVKDAITSRSKENYLNGLELMKEMGAVITNTET
                     VVFDLLKVAGTDEFRNMSKLIK"
BASE COUNT          752 a          250 c          425 g          593 t
ORIGIN      
        1 tactttgaaa tagataatta ctggtatata atgtatatag agttataaaa gctatattag
       61 gcctaaatgc aaatagaagg taaaagaaag acttaatgga aggacttaaa tcatattatt
      121 tatgggagta agaaataatt aagggggcaa gcctatgata ctgactgttg ccttagatcc
      181 tgtgttggaa aataggtatt atgtggaaaa cttatatcct aaaactatta ataaggcaga
      241 caaggttttg tacaagatat ctggtaaagg gattatctgc actagaatac tgaaaagcct
      301 taatatggat gtcttttcca ctggattttt aggtggccta aagggtaaat acatatttga
      361 agaattaaaa aggcagaata tatataatga ttttgtattt ataaaggatg agactagggg
      421 aaatctactc ttgtaccaaa aggaagagct tatctctacc attgcagagg atgggcccag
      481 gattactaga gaggaaatag ggacctttta tgaattgttt aataaattaa tagatagatt
      541 tgatctaatt tgcgcccttg gagatctacc tattggagta ccagaggaga tatattttga
      601 tttaatagac attgccaaaa agagggataa aaggtttgtc ctagatgcca agggaactga
      661 actaatcttt ggcctagaag ctgctccttt tatggctata gtagacaggg aagatttaga
      721 acagattagt aggctaaaat tagattttga gaatgaaata atcaaggttg ggataagcct
      781 attggaaatg ggaattgaag cggttattat agatttggat ggatcaggca ccatagtttt
      841 aaatcacaaa cagggatata gactttctgt accaggaatg gaaaatatga aggttaaaaa
      901 ggatcaggga tacttggccg ccggttatgt atttggcata gacaagggat acaagtttga
      961 aaccaccatg aaaataggcc aaggctttcg tctagcctac gccatgggcg atgatcgaga
     1021 tttaatagat atgggtcata ttaaaaaaat aatgaatgaa atagatattt catcaatata
     1081 ctattaggag gaattttatg gaaaagttta ctttagatag agaaaaaaca gttttattgg
     1141 ttattgatat tcaggaaaga ttggtcccgg tgatgaagta caaggatcag gtaattgaca
     1201 atacaaaaat cctattacat gcagccaggg aaatggattt tccagtggta gttaccgaac
     1261 aatatcctaa agggctggga agtactgtaa gcgaaataaa ggaagactta caggatgcaa
     1321 aaatatttgc aaagaacagc tttaccgcct atattgatga agtaaaggag agcttagagg
     1381 atatagggcg aaaaaagatt ataataactg gcatggaaac ccatatttgt gtctttcaaa
     1441 ctactagaga tctaattaag gccggatatg aggtacatat tgttaaggat gccattacct
     1501 caagaagcaa ggaaaattac ctgaatggcc tagaactaat gaaggagatg ggagctgtta
     1561 ttaccaatac agagactgtg gtctttgatc tattaaaagt ggcagggaca gatgaattta
     1621 gaaacatgtc aaagttgata aagtagctag ggaattatta aaatagttat agcattgttt
     1681 agataatatt ttcagaagct ggtatgacaa gtgtgtgatt aggtcagaag tttgcgatat
     1741 ttccgtcatc aaaatattga catgcagaaa atgatatggt aaattgacat taagattaaa
     1801 tatttcaatt atatagttaa tagacatagc tgaatataat aaatttaata actggatcac
     1861 aatatattca ctcgcatttg tgtagctttt aaatgatgcg acttccaaaa tactattacc
     1921 aatcatatta acaatatatt aataattcca cttcagaaac aataaaaaag tctttctata
     1981 gaataaaaat attcataagc gtgtaactga gttaaatcag
//
LOCUS       DATGSI010000645         4427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_140152, whole genome shotgun sequence.
ACCESSION   DATGSI010000645 DATGSI010000000
VERSION     DATGSI010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140152"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VK031_09820"
     CDS             complement(<1..488)
                     /locus_tag="VK031_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HLS54261.1"
                     /translation="MKKLVSVSLGEIALKGLNRRYFEDRLINQIIRAIKDIGYEKIYK
                     EQGKIYIEAEEKDLDIITHRVQKIFGIANISPSIRVDKDIKSIERAALQAVEDAMDSR
                     PHIETFKVETTRADKSYPMKSPEVSRHIGATVLKGMKVLKVDVHNPDLYVNIDIKQNH
                     AY"
     gene            complement(489..1646)
                     /locus_tag="VK031_09825"
     CDS             complement(489..1646)
                     /locus_tag="VK031_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HLS54262.1"
                     /translation="MIYLDNCATTKMRKEVLNKMYTALAEDFGNPSSLHRLGLKTEKK
                     VEGAREIIGDFLQVEKEEIYFTSGGTESNNLALQSIINGFKRRGKHIITSKIEHPSIL
                     NVVKNYEKLGFSVSYLNVDKNGQIKLDEFRKALRQDTILVSIIHVNNEIGTIQDIAAI
                     KRIMKEERSQALLHVDGIQSFGKIDFSLRELEVDSFSFSGHKIYGPKGVGGLYINKNL
                     KLDPITYGGNQEKGLRSGTENVPAIIALGEAVRILKERQKKEAQHVKEIRGYMIEKLK
                     ENVKDVRINTPLDEKSSPYVLSLSFINTRGEVLLHYLEDREIYVSTTSACSSKGTEKS
                     HVLQAIGLSDREIEGTIRICFSHETKKEDIDYTVEVLKEIVAEIRQIMGGD"
     gene            complement(1661..2263)
                     /locus_tag="VK031_09830"
     CDS             complement(1661..2263)
                     /locus_tag="VK031_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HLS54263.1"
                     /translation="MEKYQELVKKLEINGFTCNVFEDSYQVKKYLLEEIKLEDSIAIG
                     GSMTIKSLNLFEDFKERGNQVFWHWKADKVDEELKRAKTAKIYLTSTNALTLDGKLVN
                     MDGNGNRVASMVFGHEKVFIIVGKNKICKDYESAIERIETVAAPKNAKRLGLNTYCSQ
                     TGECNDCYTADRMCSVETIIHKNPGKTKINICLVDEELGY"
     gene            complement(2546..2713)
                     /locus_tag="VK031_09835"
     CDS             complement(2546..2713)
                     /locus_tag="VK031_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54264.1"
                     /translation="MSKLKEIMDELDAKVRKLEKDYKKMKESIKNVEEFLIKNTGGHY
                     LKSIKKKRKAP"
     gene            complement(2749..3798)
                     /locus_tag="VK031_09840"
     CDS             complement(2749..3798)
                     /locus_tag="VK031_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54265.1"
                     /translation="MAFYNDQVIIIPVGQEEIYIFKLIKEEIIMDYYNIKEGNRQEVL
                     VTDALKEFDAMVDEGRIYLIYQNKAYYLKMLIIDSKNVEEISLSEVKLPRLYELKLKK
                     IDKHHNILYMVRDGEREDYFHIHHYLLVDEKWESYQVAEVRVAKVLNPLICLNNQDKL
                     SLAYYHGNKIHLQEFNLEEKRWMEAIGLTDEGEKLYLDLLEDGNLLHLAYAQYEDNNF
                     SIKYKKFTRDSIGYHLEEEKLLSNEGNASHPTLLIQAGVLWLVWKESLNLLSRYSLDQ
                     GQSWSPLYLWKDSKALDYYRYKYVSERDIKDTKFKYSFGTLYPDVKLLGFGALENVEE
                     IPVKKKNRGYIIPRF"
     gene            3922..>4427
                     /locus_tag="VK031_09845"
     CDS             3922..>4427
                     /locus_tag="VK031_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54266.1"
                     /translation="MSSYKKPDCAHKPILPITEGCCTGTTGCEWDGTLRNVKTEPIFV
                     QKVYDAALFNMQALSTVNNVRFSPSLPRGSSVEGILDIKCRKYFNPSNIDDNRNFTID
                     PETILSGGQFLKDKHGKYVEVVGPDGLTNERLIYADTSACDKENKGTPVFGTQRLRLR
                     GNVVITMDL"
BASE COUNT         1215 a          894 c          637 g         1681 t
ORIGIN      
        1 aaataggcat ggttttgttt tatatctata tttacatata aatcaggatt atggacatcc
       61 acctttaata ccttcattcc ctttaagact gtggccccta tgtgcctaga aacttctggt
      121 gacttcatag gataagattt gtcagccctg gtagtttcca ccttgaaggt ttctatatgg
      181 ggcctgctat ccatggcatc ttctacagcc tgtagggccg ctctttctat acttttaata
      241 tctttatcta ccctaataga tgggctaatg tttgctatgc caaaaatctt ttgtacccta
      301 tgggtgatta tatctaaatc cttttcttca gcttctatat agattttacc ttgttcctta
      361 tagatctttt catagcctat atcctttatg gccctgataa tttgattaat taacctatct
      421 tcgaagtacc ttctatttaa tcccttaagg gctatttctc ctaaacttac acttactagc
      481 tttttcaact aatcacctcc catgatttgt ctaatttctg ctacaatttc ttttaaaact
      541 tccactgtat aatctatatc ctcttttttg gtttcatggg aaaaacatat tctaatggtt
      601 ccttctattt ccctatcact taggcctatg gcttgtaaaa catggctttt ttctgttcct
      661 ttagaagaac aggctgatgt ggttgataca taaatttccc tatcttctaa ataatgaagc
      721 aagacctctc ccctagtatt tataaaggat aggcttaaaa catagggaga acttttttca
      781 tctaagggtg tatttatcct tacatccttt acattttcct ttagtttttc tatcatgtag
      841 cctcttattt ctttcacatg ttgggcttcc tttttctgcc tttcttttaa gatccttaca
      901 gcttctccta gggctataat agccggcaca ttttctgtgc ctgatctcaa tcctttttcc
      961 tgattgcccc cataagttat gggatccagt tttaaattct tatttatata taagcctccc
     1021 acacccttag gtccatagat tttatggcca ctaaaggaga agctatccac ttctaattcc
     1081 cttagggaaa agtcaatctt tccaaaggat tggattccat ctacatgaag gagggcctgg
     1141 gatctttctt ctttcataat ccttttaata gctgctatgt cttgtatggt gcctatttca
     1201 ttgtttacat ggattatgct tactaaaatt gtatcctgtc ttaaagcctt tctaaattca
     1261 tctagtttga tttggccatt tttatccaca tttaaatagc ttacactgaa acctaatttt
     1321 tcataatttt ttactacatt cagaatagat ggatgttcta ttttgcttgt aataatatgt
     1381 ttaccccttc ttttaaaacc atttattatg ctttgtaaag ccaggttatt actttctgtt
     1441 cctcctgagg taaagtaaat ctcttctttt tccacctgga ggaaatctcc tattatttcc
     1501 ctggcccctt ccaccttctt ttctgttttt agtcctaatc tatgcaggga agaagggttg
     1561 ccaaagtctt cggccagggc tgtatacatc ttgtttaaaa cttccttcct catctttgta
     1621 gttgcacagt tatctaaata aatcaatctt tacacctctt ttagtaacct agttcttcat
     1681 ctaccaggca gatattaatc ttagtcttcc caggattttt atgaattatt gtttccacac
     1741 tgcacattct atcagctgta taacaatcgt tacattctcc tgtctggcta cagtaagtat
     1801 ttaggcccag tcttttagca tttttaggtg cagccaccgt ttcaatcctc tctatggcag
     1861 attcatagtc tttgcatatc ttgtttttac ctactattat aaagaccttt tcatggccaa
     1921 agaccatgga ggccaccctg ttgccattgc catccatatt gacaagtttc ccatctaggg
     1981 ttagggcatt ggtgctggtt aagtaaattt tagcagtttt agcccttttt aattcttcat
     2041 caaccttgtc tgccttccaa tgccaaaata cctggttacc tctctcttta aaatcttcaa
     2101 aaaggtttaa agactttatg gtcatggaac cgcctatggc aattgagtcc tccagcttta
     2161 tttcttccaa taaatatttt ttcacttgat aagagtcttc aaatacatta caagtaaagc
     2221 cattaatttc tagtttttta actaattctt ggtacttttc catttcagtc atcccttcta
     2281 tgcctattta ccatatatat tatagcacag ctatctagtt aggataggat ttttcagcaa
     2341 gtaattagat ttgattatcc atttttccat aatttgacaa atgctaaaaa acaattaaat
     2401 tttatcattt tttatcttgc aaagtttgtc actttttgct ataatatttc ttgaggttat
     2461 ccccctggta accttaaaag atctctattc ccaatacccc ttttgaacga ttttgttcca
     2521 tccgggtacg gatgaaaagg ctcctttatg gagcctttct tttctttttg atacttttta
     2581 aataatgacc acctgtattc ttaattagaa attcttccac attttttata ctttctttca
     2641 tcttcttata atccttctca agctttctta cctttgcatc taattcatcc ataatttctt
     2701 ttaacttact catatctatt aaaagtttat ctaatttatt ttcatcttct aaaacctggg
     2761 aattatgtag cctctgttct ttttttttac tggtatttct tccacatttt ctaaggcacc
     2821 aaagcctaat aattttacat ctggataaag ggtaccaaag ctatatttaa atttagtatc
     2881 ttttatatcc ctttcactta catatttata tctataataa tctaaagcct ttgaatcttt
     2941 ccataggtat aaagggctcc agctttggcc ttgatccaga ctatatctgc taagcaagtt
     3001 taatgattcc ttccaaacca gccacaagac tcctgcctgg atcagcaggg tagggtggct
     3061 ggcatttccc tcattggata aaagcttttc ttcttccaag tggtacccta tgctatctct
     3121 ggtaaatttt ttatacttga tgctaaaatt attgtcttca tattgggcat aggccagatg
     3181 tagaaggttc ccatcttcca ataggtccaa atataatttc tccccttcat ctgtaaggcc
     3241 tatggcctcc atccatcttt tttcctccaa attaaattct tgtaaatgga ttttattacc
     3301 atggtaatag gccaagctta gtttatcctg attgtttaaa caaattaaag gatttaaaac
     3361 cttggctacc ctaacctctg ccacttgata actttcccat ttttcgtcta ccaaaagata
     3421 gtggtggatg tggaaataat cctctctttc tccatcccga accatatata agatattgtg
     3481 gtgcttatct atttttttca atttaagctc atataatcta ggaagtttta cttcacttag
     3541 gctaatctct tctacatttt tagagtctat gattaacatt tttaaataat aggccttatt
     3601 ttgatatata aggtagattc tcccttcatc taccatggca tcaaattctt ttaaggcatc
     3661 tgttactaat acttcctgcc tattgccttc cttaatatta taatagtcca ttataatctc
     3721 ttcttttatt agcttaaata tatatatttc ttcctggcct acaggtataa ttatgacctg
     3781 atcattataa aaagccatcc tatcacctct tttagactat attattactt acataaaaaa
     3841 tttatgacag acagctagta acacatttgc aaacctttga atatattatg attgagtaat
     3901 cttttaaaag gaggtaaata tatgtcttca tataaaaagc cagattgtgc ccataagcca
     3961 atattaccaa taactgaggg gtgctgtaca ggaactacag gctgtgagtg ggatggcacc
     4021 ttaagaaatg ttaaaacaga gcctatattc gtacaaaaag tatacgatgc agccctgttt
     4081 aatatgcagg cacttagtac tgttaataat gttagatttt caccaagttt accaagggga
     4141 agttcagtgg aaggtatctt agatattaaa tgtagaaagt attttaatcc ttccaatatt
     4201 gatgacaata ggaactttac catagatcct gaaactatct tatccggtgg acagttttta
     4261 aaggataagc atggaaaata tgtggaagta gttggaccag atggactgac caatgaaagg
     4321 ttaatatatg ctgatacttc tgcctgtgat aaggaaaata aggggacacc agtatttggc
     4381 actcagagac ttagattaag aggaaatgtg gtaattacta tggatct
//
LOCUS       DATGSI010000646         1201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_324008, whole genome shotgun sequence.
ACCESSION   DATGSI010000646 DATGSI010000000
VERSION     DATGSI010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324008"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VK031_09850"
     CDS             complement(<1..1079)
                     /locus_tag="VK031_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLS54267.1"
                     /translation="MAFAYKEYETLPANISSDTSEIDMIFVGLAGMIDPPRPEAKDAI
                     RLCKQAGIVTIMITGDYKETAFAIAKELGMAEDENQAITGTELDNLSDEELREVVKTA
                     RVFARVSPEHKVRIIDALKDNGHITAMTGDGVNDALALRKADIGVSMGITGTDVAKST
                     AELILTDDNFASIVAAVEEGRVIYDNIRKFVYFLLSCNIGEIILVFASIIIGLPIPLI
                     PIQLLWLNLVTDSFPALALGVESGDPDIMKNPPRNPSEPLLNRDLMIRTVIQSIAIGI
                     STISAYLLALNFYPDNLTMARTITFTTLILAELLRAYSTRSQKHTLLEIGIFTNTTLV
                     YGTLLSFFLTLAVLYIPQMQPIFNTL"
BASE COUNT          374 a          256 c          215 g          356 t
ORIGIN      
        1 agggtgttaa atatgggttg catctgcgga atatataaga cagccaaggt taaaaagaag
       61 gacaaaaggg ttccgtaaac cagggttgtg ttggtaaaga ttcctatttc caatagggta
      121 tgtttctgtg acctggtgga ataggccctt aacaattcag ccaaaattaa agtggtaaag
      181 gtaatggtcc tggccatggt taaattatca ggataaaagt ttaaggccag caaataggct
      241 gaaatagttg atatgcctat ggcaatactt tggattacag tcctgatcat taaatcccta
      301 tttaataaag gctcactggg atttctagga gggtttttca taatatcagg gtccccactt
      361 tccacgccca aagccagtgc agggaagcta tcagtcacta aattcaacca taataattgg
      421 ataggtatca aaggaattgg cagtccaatt ataatactag caaagaccaa aataatttca
      481 cctatgttac aggataggag aaagtaaaca aatttcctaa tattatcata aataactctg
      541 ccctcttcaa cagctgccac tatactagca aagttatcat cggttaaaat taactctgca
      601 gtagacttag ccacatctgt accagtaatt cccattgaca cacctatatc tgcctttctc
      661 aaggccaaag catcattgac cccatcccca gtcatggcag taatatgccc attatccttt
      721 agagcatcta taatcctaac cttatgttct ggagaaacac gagcaaaaac tctagcagtt
      781 tttaccactt cccttaattc ctcatcagat aaattatcaa gctctgttcc agttatggcc
      841 tgattctcat cttctgccat accaagctcc ttggcaatgg caaaggcagt ttctttatag
      901 tctccggtaa tcattatagt tacaatacca gcctgtttac atagcctgat ggcatcttta
      961 gcttcaggtc ttggtggatc aatcatgcct gccagtccta cgaaaatcat atctatctca
     1021 ctggtatcag aagagatatt ggccggcagt gtttcatact ccttataggc gaaggccaaa
     1081 actcgtaagg catccttgaa aaagaaatat tttgttctag taccttttct ttcaagtctt
     1141 ggctaaaagg aattaccttt ccatccatat atatatggct acacctgttt ataataacgt
     1201 c
//
LOCUS       DATGSI010000647         4180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_420785, whole genome shotgun sequence.
ACCESSION   DATGSI010000647 DATGSI010000000
VERSION     DATGSI010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420785"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..809
                     /locus_tag="VK031_09855"
     CDS             180..809
                     /locus_tag="VK031_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54268.1"
                     /translation="MRITKVTQEKILTYFINNDELDELNTILRQQRLLRKHKIAHSLA
                     KGTRGYNILIWMHPIIEDLSKVYGKTYQEMAVMYINIGIDLMVRGYGINKFKYYNEDI
                     LNRVDQLMTVENDESPDKTERLFRYYSRKTFEHYNKKITTMTVEDQYHLIKLREFIES
                     RKINARKHIDGIFEQHNKVFPIYVLYGVKDEEVTKVDIKHSKLSKIING"
     gene            809..2155
                     /locus_tag="VK031_09860"
     CDS             809..2155
                     /locus_tag="VK031_09860"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HLS54269.1"
                     /translation="MEIRVGPYESKLIAPRNVIRAVRERMKVRVDGYQYTDAYGRGWD
                     GYKYFMTGTGTFKSGFVDSVLNILQSYEVEVVTHSDPLEVEELDTAVGNGWTLTPDQE
                     DAVRDIMNHKYRNGTPSTHGVIDAAVGSGKTSIFASLIRNFPDKDFIILVNSTDLFRQ
                     TIRFFFDIYGEDEIGIIQGPTIRLRRITVAMVQTLSRRINKEISEYFDVLIADESHNL
                     GNKTGEKVLSKLKCSYRYFFSGTAYDKDKKIDQVMLVGMCGPRIVRITNKQNQEKGIT
                     LEADVYMHEIDYPVLAKSTDYSEYLKVHPVRNTKIAQIVKSRPNQKILIVCKFIEHCE
                     NLLEYLPNNIYYTHGQDPDRHSVIDKYTKDPSGVLLTTFIIKEGINIPDIQVLISCFG
                     GQDEVSTKQVAGRAIRNDGVLDKVEIHDFYDKGGLEHHSKKRIEYYKSEGYTIKYD"
     gene            2148..2396
                     /locus_tag="VK031_09865"
     CDS             2148..2396
                     /locus_tag="VK031_09865"
                     /inference="COORDINATES: protein motif:HMM:NF023181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HLS54270.1"
                     /translation="MIKHPEHYGGKDDPYEAIKVIEAWDLCFHLGNVLKYIKRRDRKG
                     DELQNLEKAAFYINRKIENLIDERKVRKTSIIKKHPTG"
     gene            2344..2706
                     /locus_tag="VK031_09870"
     CDS             2344..2706
                     /locus_tag="VK031_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54271.1"
                     /translation="MNVKYVKPVSLRSTLPDDLPKCFGVGFDPKNTECLQCTAFNRCA
                     LDVLQKDDGNYYLDQVVEISDKDITKFIGLEKPVQEVVDYIKNTTKMKDKIAIQNRLK
                     RYIQRNETTVEGKNIKVL"
     gene            2703..>4180
                     /locus_tag="VK031_09875"
     CDS             2703..>4180
                     /locus_tag="VK031_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54272.1"
                     /translation="MRFIFDRKDDIYKLGDIIEAFENKPKYEYSTIKPPPDNFRNMDG
                     LFDKLVKTYNEEALIIVSSGYCRAHTGKSVMQWDKQPVVRGGQSIFITPSIAFLMRRE
                     ADLMDFAKVISSAMYLKSTGGEGKAFAEEVKVRTKTDIDNCIIHVKETGVVAFDFETY
                     VDDEKAQDYEWYRDDFHASLLSITPQPGISYTIPLKHFENPIPDSVREYILKQLNDGI
                     FNNPDVRKIGWNVKFDMHVAKKIGMDLRGRYDDPMVMYSILDNTVTNRRLKQVAGEYF
                     QDTIGYDTEVGAYRWKEVPLDILSKYAASDTSITILLAAVFEAQMDETLTRLYRNIVA
                     PGTKVLFEAEHRGMDVDIEVLNEAIAYSEKLEEDITKELMSYPQVIRYDEARIQREID
                     KAIKQDKAKIHKWESEEGNKEYYIERAKERIRDIKTGVREVAKPLNLASVPMMKGLLY
                     SKEGFGFKTKIKNTSEATLVDIKDDTGFIDKFLLRRKLAKNRS"
BASE COUNT         1570 a          526 c          930 g         1154 t
ORIGIN      
        1 agcatttaaa ttagcttcga agttgaaggg gaaagaagtg tatatcaagg tgtctccaga
       61 aggtgatgcg aacagtttag gtttcgaaaa ataccataaa ctagataaaa ttaaatttac
      121 atactcaaac tggatgaaat atgataagtt ataatgactt gaagaaaggt ctaaaagatt
      181 tgaggataac taaagttact caagaaaaga ttttaaccta ttttattaat aacgatgagt
      241 tagatgaact aaatactata ttgaggcaac agagactact acgaaaacat aagatagctc
      301 attcattagc taaaggtact agggggtata atatactcat atggatgcac cctattatag
      361 aggatctttc taaagtgtat ggcaaaacat accaagaaat ggcggtgatg tatattaata
      421 taggtataga tttgatggta agagggtatg ggataaataa attcaagtac tataatgaag
      481 atatattaaa tagggtagat cagttaatga cagtagagaa tgatgagtca ccggataaga
      541 ctgaaaggtt atttagatac tactcaagga aaacatttga gcactacaac aagaaaataa
      601 ccactatgac agtggaggat cagtatcact tgattaagtt gcgtgaattt atagagagta
      661 gaaaaataaa cgctagaaaa catatagatg gtatattcga gcaacacaat aaagttttcc
      721 ctatatatgt tctatacggt gtaaaagatg aggaggtaac taaagtagat attaaacaca
      781 gtaaattaag taaaataata aatggttgat ggagattaga gtaggaccat atgaatctaa
      841 gctaatagcc cctagaaacg ttataagggc agttagagag cgtatgaaag ttagggtaga
      901 tggttaccaa tataccgacg cttatggtag agggtgggat gggtataaat attttatgac
      961 aggtacgggt acttttaaat ccggttttgt tgactcggtt ctcaatattc tacaatcata
     1021 tgaggttgaa gtcgtaacac attcagatcc tttagaggta gaggagttag atacagctgt
     1081 aggtaatgga tggacgttga caccagatca agaggatgcg gttagggata taatgaacca
     1141 taaatatagg aatggtaccc ctagtactca cggagttata gatgcagctg taggtagtgg
     1201 taaaacgtct atattcgcat ctttgataag aaacttccca gacaaagatt ttataatact
     1261 agttaatagt acagacttat tcaggcaaac tataagattt ttttttgata tatacggtga
     1321 agatgagata ggaattatac aaggacctac aatccgttta cgtaggataa cggtagctat
     1381 ggtacagacg ttatcaagac gtataaataa agagatatca gagtattttg atgtcttaat
     1441 agctgatgag agccataatc taggtaacaa gacaggtgag aaagtattat ctaaattaaa
     1501 gtgtagttac cggtatttct tttctggtac agcttacgat aaagataaga agatagacca
     1561 agttatgtta gtaggtatgt gtggtccacg tatagttagg ataactaata aacagaatca
     1621 ggaaaagggt ataacattag aggctgatgt ttatatgcat gagatagatt atccagtatt
     1681 ggctaaaagt actgattatt cagaatacct aaaggtgcac ccggtgagga atactaaaat
     1741 tgctcaaata gtaaagagta gacctaatca gaagatatta atagtgtgta agtttataga
     1801 gcattgtgag aatttattag agtacctccc taataatata tattacacac atggacagga
     1861 tccagatagg cattcagtta tagataaata tactaaagat ccttcgggag tattattgac
     1921 gacttttata ataaaggaag ggataaatat accggatata caggtattaa tatcttgttt
     1981 tggtggtcaa gatgaggtta gtacaaagca ggtagcaggt agagctatca ggaatgatgg
     2041 tgtgttagat aaagtagaga tacacgattt ttatgacaaa ggagggttag aacaccattc
     2101 taaaaaacgt atagaatatt ataaatcaga aggttataca ataaaatatg attaaacatc
     2161 cagaacatta tgggggtaaa gatgaccctt atgaagctat aaaagtaatt gaggcctggg
     2221 atctatgctt ccatttaggc aatgttttaa agtacattaa aaggagagat agaaaaggtg
     2281 acgaattaca gaatttagag aaggccgcat tttatataaa caggaaaata gagaatctaa
     2341 tagatgaacg taaagtacgt aaaaccagta tcattaagaa gcaccctacc ggatgattta
     2401 cctaagtgtt tcggagtagg gttcgaccct aaaaatacag agtgcctaca atgcacagca
     2461 tttaaccggt gtgctctaga tgtgttacaa aaggacgacg gtaattacta cttagatcaa
     2521 gtagtagaaa tatccgataa agatataacc aaatttatag ggttagagaa accagtacag
     2581 gaagtagtag actatataaa aaatactacg aaaatgaagg ataagatagc tatacagaac
     2641 aggctgaaga gatatataca gagaaatgaa actacagtag agggtaaaaa tattaaggta
     2701 ctatgagatt tatattcgat cgtaaggatg acatatataa gttaggtgat attatagagg
     2761 cttttgaaaa taaacctaag tatgagtata gtactataaa gccgccacca gacaattttc
     2821 gtaatatgga tggtttattt gataagttag taaaaacgta taacgaagaa gcgctcataa
     2881 tagtgagttc aggttattgt agggcacaca ctggtaagtc ggttatgcag tgggataaac
     2941 aaccagtggt tagagggggt caatctatat ttataacccc gtctatagct tttctaatgc
     3001 gtagagaagc tgatcttatg gacttcgcta aagttataag ctccgctatg tatctgaaat
     3061 ctaccggagg agaaggtaag gccttcgctg aggaggttaa ggttagaact aaaacagata
     3121 tagataactg tattatacat gttaaagaga cgggtgtagt agcttttgac tttgagactt
     3181 acgtagatga cgagaaagcg caagattatg agtggtatag ggatgatttt catgcttcgt
     3241 tgcttagtat cacaccgcaa ccaggtataa gctatactat accgttaaaa cactttgaga
     3301 atcctatacc agatagtgtt agagagtaca tactaaagca gttgaatgac ggtatattta
     3361 ataatccaga tgtaaggaaa atagggtgga acgttaagtt tgatatgcat gtagctaaga
     3421 aaataggtat ggatttaagg ggtagatatg atgaccctat ggtcatgtat tctattttag
     3481 ataatacagt gaccaatagg agattaaagc aagtagctgg agaatacttt caagatacta
     3541 taggttacga tacagaggta ggggcataca ggtggaagga ggtacctttg gatatattga
     3601 gtaagtatgc tgcttcggat actagtatca caatactact agcagcggta tttgaggctc
     3661 aaatggatga gacattaact aggttatata gaaacatagt agcaccagga accaaagttt
     3721 tgtttgaggc tgagcataga gggatggatg tagatataga ggtgttgaat gaagctatag
     3781 catactcaga gaaattagag gaggatataa ccaaagaatt aatgagttat ccccaggtta
     3841 taaggtacga tgaagccagg atacagagag aaatagataa agctataaaa caggataaag
     3901 caaaaataca taaatgggag tcagaagaag gtaataaaga gtattatata gaacgagcta
     3961 aagagcgtat tagagacatt aaaacaggcg ttagagaagt agctaaacca cttaacttag
     4021 catctgtacc tatgatgaag ggtttgttat actctaaaga aggtttcggg tttaaaacta
     4081 agattaaaaa cacctcagaa gctactctag ttgatataaa ggatgataca gggtttatag
     4141 ataagttctt attacgtaga aagctagcta agaacagatc
//
LOCUS       DATGSI010000648         6117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_40969, whole genome shotgun sequence.
ACCESSION   DATGSI010000648 DATGSI010000000
VERSION     DATGSI010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40969"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..581)
                     /locus_tag="VK031_09880"
     CDS             complement(27..581)
                     /locus_tag="VK031_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HLS54273.1"
                     /translation="MNTRYITKASLIAAIYIVLVLIQMLPAPFVNLTFGPIQLRLAEG
                     LTLLPLVEAAAVPGLFVGCLLANLLLAAYSGFGLIDIIGGSAVTLLAAYLTYKVGQST
                     IQARGKEGRGIFKTRISLKGVILGMIPPVALNGLIVSIWVSYFTKVPYIITVLGIAGG
                     ELASVIFGILTLVAYEKATGFKEY"
     gene            complement(683..2344)
                     /locus_tag="VK031_09885"
     CDS             complement(683..2344)
                     /locus_tag="VK031_09885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HLS54274.1"
                     /translation="MSKKSKLSIIPLGGLGEIGKNITVFEYENDIIVVDCGMTFPEDE
                     MLGVDIVIPDITYLIKNKDKIRGMVLTHGHEDHIGAIPYALKKIDMPIYGTKLTLGLL
                     ENKLKEHRLEKTRLNVVKHGQTIRLGKFGIEFIKTGHSIPDSASLAIHTPVGTVVHTG
                     DFKIDFTPISGDVTDLHKFAELGQKGVLALLADSTNAERPGFSMSESTVGDTFNGIFS
                     NTKGRIIIATFASNVHRIQQIIDAAEKHKRKVVLSGRSMINTTTVASELGYLHFKDGT
                     LIDINDMNKYRKDEIVIITTGSQGEPMSALTRIAFSEHRRISLLEDDLVILSASPIPG
                     NEKTISRVINKLIENGTEVIYETLADVHVSGHARQEELKLIQNLVKPKYFIPVHGEYK
                     HLKSHKDIAKSMGMREENIFLLENGQSLDFTKDGARLGQKVPSGNILVDGLGVGDVGN
                     IVLRDRKHLSEDGLIVVVVTMSKQEGKVLAGPDIISRGFVYVRESEDLMEESRERVKR
                     VLEECERKNITDWATLKYNIRDALRSYLYEKIKRNPMILPIIMEV"
     gene            complement(2414..2857)
                     /locus_tag="VK031_09890"
     CDS             complement(2414..2857)
                     /locus_tag="VK031_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HLS54275.1"
                     /translation="MELDIREYKETLRREGYKLTDQRKAILSTIIEHHEEHLSCDEIF
                     NLVSKKYPDIGIATVYRTLQLFEKLNIVYKLNFDDGFSRYEINYGTGEHHHHHLICVK
                     CGEVLEVQLDLLEALEKEIENRGIFTIIDHNVQFYGFCNDCNKDV"
     gene            complement(2911..3159)
                     /locus_tag="VK031_09895"
     CDS             complement(2911..3159)
                     /locus_tag="VK031_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HLS54276.1"
                     /translation="MGERFLFYDELGNKIEFLVKAKFTLDDTDYAALLPADDIESEVY
                     ILRIELDKNGDPVFASIDGSELEEAIETYEQLLKENLQ"
     gene            complement(3161..3583)
                     /gene="ruvX"
                     /locus_tag="VK031_09900"
     CDS             complement(3161..3583)
                     /gene="ruvX"
                     /locus_tag="VK031_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HLS54277.1"
                     /translation="MERIIGLDVGDKTIGVAISDLLHLTAQGLTTIRREGKRKDFQKL
                     EDIIDEYDVKKIVIGLPKNMNGTIGPQGEKVLKFGEKIKNKFNIDLIYMDERLTTVAA
                     ERILIEGDVRRENRRKLIDKVAATYILQSYLDSNRKEC"
     gene            complement(3885..4148)
                     /locus_tag="VK031_09905"
     CDS             complement(3885..4148)
                     /locus_tag="VK031_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HLS54278.1"
                     /translation="MEKDLDKTMMFKISKDVDMEPREIISHVYNALEEKDYDPINQII
                     GYILSGDPTYITSHKNARSIIRKLERDELLEEILKKYMESEKK"
     gene            complement(4248..>6117)
                     /gene="alaS"
                     /locus_tag="VK031_09910"
     CDS             complement(4248..>6117)
                     /gene="alaS"
                     /locus_tag="VK031_09910"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HLS54279.1"
                     /translation="DLSNYKYGNDDKKDISIRVITDHARAMTFLVSDGVLPSNEGRGY
                     VLRRLIRRAARHGKLLGIEGEFLAEIVKEVIDSWKVAYKDLVTRQEQIERVIKAEEDK
                     FQETIDQGIFILENYIKEMEDEGQKILDGDKAFKLYDTYGFPIDLTKEILEEKSLSVN
                     LVEFNTKMEEQRDRARRARQEINSGWKDNQLNDLLKDLESSFTGYEEIKTHTEIIGLF
                     KDNERVGVLEREDEGILILKKTPFYGEAGGQVGDIGVIYNENFTAKVLDTSLSNNDLT
                     LHMVKLKKGSVAEGDKVVAKVEDLNRDSIKRNHSATHLLHKALKEVLGEHVNQAGSLV
                     LPNRLRFDFTHFEAVSQRELEEIENRVNEKILKALTVSTEVTSLDRAEKLGAIGLFED
                     KYEDEVRVVKMGDYSIELCGGTHVDNTANIGLFKIISEGGIASGVRRIEAITGLEVYN
                     YLKELEKDMDKLTVVLKTNREHLQDKASSMVEELREKDREIEKLKAKLATDIAKDIIE
                     EAKYVEDIPIVSYKTDSMDVNALRNLGDEIRNRLDSAVVVLASTQNSKLVFISMVTKD
                     LIDKGILAGNLVREVAKVTGGNGGGRPDMAQAGGKDISKLDEALDLVPDLVKKQLK"
BASE COUNT         1776 a         1353 c          773 g         2215 t
ORIGIN      
        1 ttttttcctc tccaatctat tgaaaactaa tattccttaa aacctgtggc cttctcataa
       61 gctaccaatg ttaaaatacc aaatattacc gaggccagct caccgccagc tatacctaaa
      121 actgtaatta tatatgggac ctttgtaaag taggataccc aaattgatac aattaatcca
      181 ttaagtgcca ctggtggtat catgcctaaa atgacgccct ttaggcttat tctggtttta
      241 aaaatgcccc ggccttcctt tcccctggcc tgtatagtac tttggcctac cttataagtt
      301 aaataggcag ccagtaaggt aactgcactt ccacctataa tatctattaa accaaaacct
      361 gagtaggctg ccaaaagtaa atttgccaac aaacaaccta caaagagtcc aggaactgct
      421 gcagcttcta ccaagggtaa aagtgttagt ccttccgcca atcttaattg gataggtcca
      481 aaggtaagat ttacaaaagg tgccggcaac atttgaatca atactaaaac aatgtatatg
      541 gcagcaatta gactagcttt agttatatat cttgtgttca tactctacct tcctttccaa
      601 ttttaaatca tcttccatta taccattatt ttttaatatt aaaaagctaa atccccggta
      661 tatttaatat accagggatt aattaaactt ccataataat aggcaatatc attggatttc
      721 ttttaatctt ttcatataga taggatctga gggcatctcg gatattatac tttaaagttg
      781 cccaatcggt aatgttcttc ctttcacatt cttctagaac cctttttact ctctctcttg
      841 actcttccat taagtcttca gactccctaa catatacaaa gcctcttgag attatatctg
      901 ggccggcaag taccttgcct tcttgcttgc tcatagtaac tactacaact attaagccat
      961 cctcagatag gtgtttccta tctcttaaaa caatatttcc aacatctcct actcctaggc
     1021 catccaccaa aatatttcca gaaggcacct tttgccctaa tctagcccca tccttagtaa
     1081 aatctaggct ctgtccattt tccaatagga aaatattttc ttctctcatt cccatagact
     1141 ttgcaatatc cttatggctc tttaagtgtt tatattcgcc atggacaggt atgaaatatt
     1201 tcggtttgac taaattttgg attaacttta attcttcttg acgggcatgg cctgaaacat
     1261 gaacatcggc taaagtctca taaattactt ctgtgccatt ttctattagt ttatttatta
     1321 cccttgaaat tgttttttcg ttacctggta taggtgaagc cgataatata actaaatcat
     1381 cttcaagcaa tgaaatcctt ctatgctctg agaaggcaat tctagttaga gccgacatgg
     1441 gctccccttg ggatcctgta gttataatta ctatttcatc tttcctatat ttattcatat
     1501 cgttaatatc tatcaaggtt ccgtctttaa aatgcaaata tccaagttca ctggcaacag
     1561 tagtagtatt tatcatagac ctacctgata agaccacttt tctcttatgt ttttcagctg
     1621 catcaataat ctgttgaatt ctatgcacat ttgaagcaaa agttgcaatg attatccgac
     1681 ccttagtgtt agaaaaaatg ccattgaagg tgtcacctac cgtactttca ctcatggaaa
     1741 aacctggcct ttcagcatta gtactatccg ccagtaaggc taaaacacct ttttgcccta
     1801 actcagcaaa cttgtgtagg tcagttacat ctcctgaaat aggggtgaaa tctatcttga
     1861 aatcaccagt atgaactaca gttcctaccg gcgtatgaat ggctaaagat gcactgtctg
     1921 gaatgctgtg tcctgttttt ataaattcaa ttccaaattt tcctaatcta attgtctgtc
     1981 catgttttac aacatttaag cgtgtttttt ccaacctgtg ctcttttagc ttgttctcca
     2041 atagacctaa ggttagtttg gtaccataaa taggcatatc aatctttttt aatgcataag
     2101 gtattgcacc tatatggtcc tcatggccgt gggttaatac cataccacgg attttatcct
     2161 tattttttat taaatatgtt atgtctggaa taacaatatc cacacctaac atttcatctt
     2221 ctggaaaggt cattccacaa tctactacaa ttatatcatt ctcatattca aaaactgtta
     2281 tattcttacc aatttcccct agtcctccta agggaataat acttaactta ctttttttac
     2341 tcaccgtatc actcctgttt attgtttact ttgtactatt ctaattataa ttgtctaatc
     2401 ttaacaagaa aatttataca tccttattac aatcgttgca aaatccataa aactgtacat
     2461 tatggtcgat tatagtaaaa attcccctat tctcaatttc tttctctaag gcctccaaca
     2521 gatccaattg tacttctaaa acctctccgc attttacaca aattaagtgg tgatggtgat
     2581 gttccccagt accataatta atctcatacc tgctaaatcc atcatcaaag tttagtttat
     2641 atacaatatt aagtttctca aaaagttgta gagtcctata cactgttgca ataccaatat
     2701 ctggatattt tttagagaca aggttaaaaa tctcatcaca gcttaaatgt tcctcatgat
     2761 gctcaataat agtgcttaga atggcttttc tctgatctgt taacttatat ccttcccgtc
     2821 ttaaggtttc tttatattcc cttatatcaa gctccattaa tttcacccgt ccatatcaac
     2881 ttattaataa tatgcctaca aatcttttac ctattgcaaa ttttctttca gcaactgttc
     2941 ataagtctct attgcttctt ccaattcact accgtctata ctggcaaata caggatcccc
     3001 attcttatct agttctatac gcaatatata aacttctgat tctatatcat cagccggcaa
     3061 caaagcggca taatcagtat catccagtgt aaattttgct ttcactaaaa attcgatttt
     3121 gtttcccagt tcatcataaa acaaaaacct ttctcccata ctaacactcc ttcctatttg
     3181 aatctaaata agactgtaat atatatgtgg ctgccacttt gtctattaat ttcctcctgt
     3241 tttctctcct aacatctcct tctatcagga ttcgttctgc tgccacagtt gtcagccgtt
     3301 catccatata tataagatct atattaaact tattctttat tttctcccca aatttaagca
     3361 ccttttcacc ctgcgggcca atggttccat tcatattttt tggcaaacca attactattt
     3421 tctttacatc atattcatca attatatctt caagtttttg aaagtctttt cttttccctt
     3481 cccgtcgaat tgtagtcagc ccctgggctg ttaagtgcaa taaatcacta atagcaacac
     3541 caatagtttt atctccaaca tctaatccta ttattctttc catttctact cctttagtat
     3601 atccttaact tttcaattac caagcataaa ataacaagcc ctatttaggt gaccttactt
     3661 atatataata aggccaccta aatagacttt tcctttgtgt tggatagact taactttatt
     3721 taatattatt atacataaca atgtcttctt taataattta aatacagaaa ataaaattta
     3781 agtaaaataa tttattttaa atttattgaa aaactatgaa atttctaatt tacaagcata
     3841 ttaatcttta taatagctga agcataaaaa tatttagaga aatattactt cttttcactt
     3901 tccatatact tctttaaaat ttcttctaac agctcatctc tttccagctt cctgattata
     3961 gatctagcat tcttatggct ggtaatataa gttggatctc cagacaggat atatccgata
     4021 atttgattaa taggatcata atccttttcc tctaaggcat tgtatacatg agaaataatt
     4081 tctctaggct ccatatccac atccttggaa atcttaaaca tcatggtttt atctaaatct
     4141 ttttccatgg ctacaccccc acttagtttt attataccat aaaatcgaag acttcccagc
     4201 atgaaaaaat attagcctaa gctaatattt tttctgctag gttaaaatta cttcaattgt
     4261 tttttgacca aatcaggtac cagatctaaa gcttcgtcca gctttgaaat gtcttttcct
     4321 cctgcctggg ccatatccgg tcggcctccg ccgtttcctc cagttacctt ggcaacctcc
     4381 ctaactagat ttcctgctaa aattccctta tctattaaat ctttcgtaac catagatatg
     4441 aaaacaagtt ttgaattttg agtagaagcc aaaacaacca ctgcagaatc cagcctattt
     4501 ctgatttcat ctcccaggtt tcttaaggca tttacatcca tactatcagt tttatagctg
     4561 actataggaa tatcttcaac atatttggcc tcttctataa tatctttagc tatatctgta
     4621 gccaacttgg cctttaactt ttcaatttcc ctatctttct ccctaagctc ttcaaccata
     4681 gaactggcct tatcttgtaa gtgttcccta ttagttttta acacaactgt taatttatcc
     4741 atatcttttt ccagttcttt caaatagttg taaacctcaa ggccagtaat tgcttcaatc
     4801 cttcttactc ctgaagcgat tccgccttcg gaaatgattt taaataaacc aatattggct
     4861 gtattatcca catgggttcc accacataat tctattgaat aatcccccat tttaactact
     4921 cttacttcat cttcatactt atcttcaaaa agacctatag ctcctaattt ttcggcccta
     4981 tctaaggagg tgacttcagt agaaacagtt aaggctttta atatcttttc attgacacga
     5041 ttttctatct cctctaattc cctttggctc actgcttcaa aatgggtaaa gtcaaatctt
     5101 aatctattag gtagtaccag ggagccggcc tggtttacat gttcgcctaa aacttctttt
     5161 agggccttgt gaagtagatg ggtagcagag tggtttcttt ttattgagtc tcgattcagg
     5221 tcttctacct tggctactac cttatcccct tcagccactg agcctttttt taatttgacc
     5281 atatgaagtg tcagatcatt gttcgataaa ctagtatcta aaaccttggc tgtaaaattt
     5341 tcgttgtata taacacctat atctccaacc tgtcctccag cctccccata aaatggggtc
     5401 tttttaagaa ttaggattcc ctcatcttcc ctttctaaaa ctcctaccct ttcattatcc
     5461 ttaaataagc caattatttc agtatgggtc ttaatttctt catatccggt aaaggaagat
     5521 tctaagtctt ttaataaatc attaagctga ttatccttcc agccactgtt tatttcctgt
     5581 ctagcccttc ttgccctatc tctttgctct tccatcttag tattgaactc tactaaatta
     5641 acacttaatg atttttcctc cagtatttcc ttagttaaat ctataggaaa accataagta
     5701 tcatataatt taaaggcctt gtctccatct aatattttct gaccttcatc ttccatttcc
     5761 ttaatataat tttctaggat aaagatacct tgatctatag tttcttggaa tttatcctct
     5821 tcagctttaa taaccctttc aatttgttct tgtctagtta ctagatcctt gtaggccacc
     5881 ttccaggaat ctataacttc cttaactatt tcggctaaaa attcgccttc tattcctagt
     5941 aattttccat gacgagcagc ccttctaatc aaccttctta gaacatagcc tcgtccctca
     6001 ttactgggca aaactccatc tgaaactaaa aaggtcatgg ccctagcatg gtcagtaata
     6061 accctaattg aaatatcttt tttgtcatca ttaccatatt tatagttact taaatct
//
LOCUS       DATGSI010000649         1690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_705797, whole genome shotgun sequence.
ACCESSION   DATGSI010000649 DATGSI010000000
VERSION     DATGSI010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705797"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="VK031_09915"
     CDS             complement(<1..1417)
                     /locus_tag="VK031_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLS54280.1"
                     /translation="MGLLRRIFGTYSSRELKKIDHLVEEILDLEDHMQNLSDEQLRDK
                     TTEFKDRLDKGETLDNILPEAYAVVREASWRVLGMKHFKVQLYGGIILHQGRIAEMKT
                     GEGKTLVATLPAYLNGLTGKGVHIVTVNDYLAKRDRQWMGKVYEFLGLSVGCIVHGLS
                     NQERKEAYRADITYGTNNEFGFDYLRDNMVIYKEEMVQRPLYYAIVDEVDSILVDEAR
                     TPLIISGAGDKSTDLYKRADNFVRGLTSRVISPEEEKQDPFDREIKEETVDFLIDEKA
                     KTCSLTEKGTAKAEAYFKVENLADMENMEIAHHINQALKARNIMKRDIDYVVKDGEVV
                     IVDEFTGRLMYGRRYSNGLHQAIEAKEGLDVRSESKTLATITFQNYFRMYKKLAGMTG
                     TAKTEEAEFREIYNMDVIEIPTNKPVIREDFPDSVYKNEKGKFRAVVEEIKERHKTGQ
                     PILVGTITIEKSELLSKMLKRE"
     gene            complement(1567..>1690)
                     /locus_tag="VK031_09920"
     CDS             complement(1567..>1690)
                     /locus_tag="VK031_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54 modulation/S30EA ribosomal C-terminal
                     domain-containing protein"
                     /protein_id="HLS54281.1"
                     /translation="ILQMELLGHNFFVFRDADTDDINVIYKRKDGNYGLIEPEI"
BASE COUNT          450 a          398 c          226 g          616 t
ORIGIN      
        1 cctctctctt aagcatttta cttaatagct ctgatttttc aatggtaatg gttcctacta
       61 aaataggctg gccagttttg tgcctttcct taatttcttc aactactgcc ctaaatttac
      121 ccttctcatt tttgtagaca ctatctggaa agtcttccct gattactggc ttattagttg
      181 gaatctctat tacatccatg ttatagattt ccctaaactc agcctcttcc gttttggctg
      241 tccctgtcat tccagccagt tttttataca ttctaaaata attttggaag gtaattgtag
      301 ccaaggtctt agactcggat cttacatcta gcccttcttt agcctctatg gcctgatgta
      361 gtccattgct gtatcttctg ccgtacatca gtcttccagt aaattcatct acaattacaa
      421 cttccccatc ttttaccaca tagtctatat ctcttttcat tatattccta gcctttaagg
      481 cctggttaat atggtgggca atttccatat tttccatatc tgccaaattc tctaccttaa
      541 aataggcttc tgccttagca gttccctttt ctgttaagga acaggtctta gccttttcat
      601 cgattagaaa atcaactgtc tcttctttaa tctccctatc aaaggggtct tgcttttcct
      661 cttctgggct tataactcta gatgttagac ctctaacaaa attgtctgcc cttttgtata
      721 agtcggtgga cttatctcca gccccagaaa ttatcagggg agtcctggcc tcatctacta
      781 aaatactatc cacctcatcg actatggcat agtacaaggg cctttgaacc atttcttcct
      841 tataaattac catattgtcc cttaaataat cgaagccaaa ctcattgttt gttccatagg
      901 taatatctgc cctataggct tcctttcttt cttggttgga caggccatgg actatacagc
      961 ctacacttaa acctaaaaat tcatatacct tgcccatcca ttgcctatct cttttggcca
     1021 ggtaatcatt tactgtaaca atatggacgc ctttaccagt taggccattt aaataggccg
     1081 gtaaggttgc aaccaaggtc ttaccttccc ctgtcttcat ttccgctatc ctaccttggt
     1141 gcaatattat gccaccataa agctggacct taaaatgttt catacctaat actctccagg
     1201 aagcctctct taccactgca taggcctctg gtaaaatgtt gtctaatgtt tctcccttat
     1261 ctaatctatc cttaaactct gtagtcttat cccttagctg ttcatctgat aaattttgca
     1321 tatgatcttc tagatccaaa atttcctcaa ctaaatgatc aatttttttc aattctcgag
     1381 aactataggt gccaaatatt cttctcaaca agcccatgac ttattaacca cctttcaata
     1441 tataacctaa cttattttat catttataaa aggctctttc aactagagag tataattggc
     1501 cctattagtt tataaaccta caaaaaaata aaagttgtag aaatctacaa cttttatatc
     1561 tatatattaa atttctggtt ctattaaacc atagtttcca tcttttcttt tatagattac
     1621 atttatatca tcagtgtctg catctctgaa tacaaaaaag ttatggccta acagttccat
     1681 ctgcaggatt
//
LOCUS       DATGSI010000650         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_705798, whole genome shotgun sequence.
ACCESSION   DATGSI010000650 DATGSI010000000
VERSION     DATGSI010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705798"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..669
                     /locus_tag="VK031_09925"
     CDS             40..669
                     /locus_tag="VK031_09925"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HLS54282.1"
                     /translation="MRKHEVLQRIEDVGVVAVVRAENSQEAKKIALACMEGGIDSIEI
                     TFTVPGADKVIEALNEEFGDKLLVGAGTVLDSETARIAILAGAKYIVSPGFDLDTAKL
                     CNRYQIPYMPGCMTITEMIKAMEAGADVIKVFPGSAFGPSFIKAIKGPLPQAVLMPTG
                     GVSIDNVGEWIKNGCIAVGVGGNLTKGTSEDMTNAAKEFVAKVKEARSQ"
     gene            689..1717
                     /locus_tag="VK031_09930"
     CDS             689..1717
                     /locus_tag="VK031_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HLS54283.1"
                     /translation="MKKGKVVTLGEIMLRLTTPRYERFVQAESFDVVYGGGEANVAVS
                     LTNYGYEGHFVTKLPKHEIGQAAVNHLRRFGVNTDYIVRGGDRVGIYYLETGASMRPS
                     KVIYDRAHSAIAEADASEFDFDEIFKDAKWFHFSGITPALGEKAAKLTEEALKAAKKH
                     GVTTSVDLNYRHNLWTPEEAQKVMTKLMEYVDVCIGNEEDAHLTLGFKPGETDVTSGE
                     VDLAGYESIFKQMMEKFNFKYVISSLRESYSASDNGWSACAYDGNEFYHSRKYDIRIV
                     DRVGGGDSFASGVIYGLLEGKNFKDALEFGVAASALKHTIHGDFNMVTVEEVENLLGG
                     DASGRVQR"
     gene            complement(2078..>3020)
                     /locus_tag="VK031_09935"
     CDS             complement(2078..>3020)
                     /locus_tag="VK031_09935"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLS54284.1"
                     /translation="VTSYLPSTGADALDTIKGQLREGRKAMENYHPNKGADILGFHLE
                     GPFINKEYRGMQKEEYCLNPSIDILEGFFQVAGRENIKLITLAPELDGSLEFIKYVNR
                     EGIQISIGHSAAEFEDIRDLKDYGLGGVVHTYSGMRGFHHRRLGVVGAVMYFDDLYAE
                     FAKQTGMTVKPEAFAIMYKIKGPEKIILTSDSTGLAHVRQPFYHYIRKCNFIPDGDYV
                     KLVYDDGKEERIYKYDYERVKDLELSYIDSVKNVVTNIDASIGDIVKMTAENPAKYIG
                     VYDRKGSIEIGKDADLLVIDGLWNIKDVYVKGVKQEI"
BASE COUNT          974 a          559 c          567 g          920 t
ORIGIN      
        1 attagtttcg cagtatagaa caccaaggag gtcgattata tgagaaagca tgaagttctt
       61 caaagaattg aagacgtagg cgttgtagct gtagtccgtg ctgaaaatag ccaagaggct
      121 aagaagattg ccttagcttg catggagggt ggcattgatt caatagaaat cacctttaca
      181 gtacctggcg cagacaaagt tatagaagct cttaatgaag agtttggtga caaacttctg
      241 gtaggagcag gcacagttct tgatagcgaa acagcaagaa tagctatctt agctggtgcc
      301 aagtatatag taagtccagg ctttgattta gatacagcca aattatgcaa taggtatcaa
      361 attccatata tgcccggctg catgactatt acagaaatga taaaagccat ggaggctgga
      421 gcagatgtaa ttaaagtatt cccaggaagt gccttcggac catcctttat aaaggcaatt
      481 aagggtcctt taccacaggc tgttttaatg ccaacaggtg gcgtaagcat agacaatgtg
      541 ggagaatgga ttaagaatgg atgtatagct gtaggcgttg gtggtaactt aactaaggga
      601 actagtgaag atatgactaa tgctgccaaa gaatttgtag caaaggttaa agaggccaga
      661 agccaataaa attaacggag ggataaatat gaaaaaaggt aaagttgtaa ctttaggtga
      721 aatcatgtta aggcttacaa ctcctagata tgaaagattt gttcaagcag aatctttcga
      781 tgtggtatac ggtggtggag aggcaaatgt tgctgtatct ctaactaact acggatatga
      841 aggacatttt gttaccaagc ttcctaagca tgaaattggg caagctgctg taaaccactt
      901 aagaagattt ggagttaata ctgattatat tgtaagaggt ggagacaggg ttggtattta
      961 ctacctagaa acaggtgcct caatgagacc ttcaaaagtt atttatgaca gggctcattc
     1021 tgcaatagct gaagcagatg catctgaatt tgactttgat gaaatcttta aagatgctaa
     1081 atggttccac ttctcaggaa ttacgcctgc cttaggtgaa aaagcggcta agttaactga
     1141 agaggccttg aaagctgcta aaaaacacgg ggtaactact tcagtagact taaactatag
     1201 gcacaaccta tggactccag aagaagcaca aaaagtaatg acaaagttaa tggagtatgt
     1261 agatgtatgt ataggtaatg aagaggatgc tcacctaaca ttaggattca aaccaggtga
     1321 aacagatgtt acttcaggtg aagtagatct ggccggatat gaaagcatat ttaagcaaat
     1381 gatggagaaa ttcaacttta aatatgttat ctcttcctta agagaatcct actcagcatc
     1441 agacaatggc tggtcagctt gtgcctatga tggaaatgaa ttctaccact ctagaaaata
     1501 cgatattaga atcgtagaca gggttggtgg aggagattcc ttcgcatcag gcgtaatcta
     1561 tggattacta gaaggcaaaa acttcaaaga tgctttagag tttggtgtag ctgcatcagc
     1621 attaaaacac acaatccatg gtgactttaa catggtaaca gtagaagaag ttgaaaactt
     1681 attaggcgga gacgcttcag gtagagtgca aagataatta tatataaata aaagaaaact
     1741 tattcattaa ccttccaaaa caggagtcct aggactcctg tttccttttt ggtatccatc
     1801 tttcagctat accttaggaa ccaatataag gctgtatcaa attttacata ttaaatatat
     1861 taatagctcc caaaaataat aaaaacttag actttcccaa tattacttct ataaaaactt
     1921 ttcagataaa taaggtctta aagggtttat tattgctaga tttgttcata tattatatgt
     1981 ggctttcaaa ccatgtatga ttacatattt taagaattat tatataagct acaaatttaa
     2041 aaggcagcct gatagactca ccagactgcc tttcatccta tatctcttgc tttacacctt
     2101 ttacgtatac atctttaata ttccacaatc catctattac taaaagatct gcatcttttc
     2161 ctatttctat agatcccttc ctatcataca cccctatata ttttgcagga ttttctgcag
     2221 tcatcttcac tatatctcct atggatgcat ctatattagt tacaacattt ttaactgaat
     2281 ctatatagct taattctaaa tcctttactc tctcataatc atatttatag atcctttctt
     2341 ccttaccatc atcataaact aactttacat aatctccatc tggaataaag ttgcattttc
     2401 tgatgtaatg gtaaaatggc tgtcttacat gagccaaccc tgtagaatca gaggtcaaga
     2461 ttatcttttc tggtcccttt attttataca ttatggcaaa agcctcaggc tttacggtca
     2521 tgcctgtttg ttttgcaaat tcagcataga gatcatcaaa atacatgact gcccctacaa
     2581 ctcctaacct tctatgatgg aatcctcgca ttccactata ggtatggacc accccaccta
     2641 agccataatc cttcaaatct cttatatcct caaattcggc agcactatgc cctattgaga
     2701 tttgtattcc ttccctattg acatatttta taaattccaa tgagccatcc agctccggtg
     2761 ccaaggttat caactttatg ttttctcttc cggctacctg gaaaaaacct tccaatatgt
     2821 ctatggaggg gtttaaacaa tattcctctt tctgcatccc cctatattcc ttgttaatga
     2881 atggcccttc taaatggaaa cctagtatat ctgcaccctt gttaggatgg tagttttcca
     2941 tagcctttct accttctctt aattgccctt taatagtatc taaagcatct gcacctgtgg
     3001 agggtaagta agaagtaacc
//
LOCUS       DATGSI010000651         1178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_612229, whole genome shotgun sequence.
ACCESSION   DATGSI010000651 DATGSI010000000
VERSION     DATGSI010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612229"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VK031_09940"
     CDS             <1..229
                     /locus_tag="VK031_09940"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HLS54285.1"
                     /translation="DGKYIVFVEVKTRTNTAFGYAFEAVNYPKQKRIMKVSLAFLKQE
                     GINNRQVRYDIIEVYLQEETKINHIENAFCL"
     gene            305..>1178
                     /locus_tag="VK031_09945"
     CDS             305..>1178
                     /locus_tag="VK031_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HLS54286.1"
                     /translation="MYSKLMTCVLQGLNGYIVEVETDLSRGLPAFNIVGLPDAAIKES
                     KERVRASIKNSGFVFPLNRITINLAPANLRKEGSQIDLAIAVGILASSGMVRQEKLSD
                     TVLLGELSLDGKLNRIDGALPMVISMREENIKSCIVPYDNREECGLITDIEIIAVKNL
                     KEAVAYLNGEKEILPYKGKEIQWDIGEEHKLDYSDIRGQESLKRALEIAAAGSHNCIL
                     VGPPGSGKTMAARRLPTILPKLSFEESIEVTKIYSVAGLMDSKGLIMERPFRSPHHTS
                     SSAALIGGGRIPKPG"
BASE COUNT          409 a          184 c          268 g          317 t
ORIGIN      
        1 ggatgggaag tatattgttt ttgttgaagt taaaacaagg acaaatactg cttttggtta
       61 tgcatttgag gctgtaaatt accctaagca gaaaaggata atgaaggtgt cactggcatt
      121 tttaaaacag gaaggaataa ataacaggca ggtaaggtat gacataatag aggtatatct
      181 acaagaagaa acaaaaatca accatattga gaatgcattt tgtctataaa gaggtgaaaa
      241 tggctattta ccactattac ccaggcaatc tatatttcaa tagaaagata ggtgagagat
      301 aaaaatgtat tccaaattaa tgacttgtgt gttacaaggc ttaaatggtt acatagtgga
      361 agtagaaacc gatctatcca ggggactacc agcatttaat attgttgggt taccagatgc
      421 agctattaaa gagtcaaagg aaagggtaag ggcttccata aagaatagtg gctttgtttt
      481 tcctctaaac cggattacca ttaatttggc tccagctaac ctaagaaagg aaggctccca
      541 aatagatttg gcaatagctg taggtatttt agcttcaagt ggtatggtaa ggcaagaaaa
      601 gttatcagat actgttttac taggtgaatt atccttagat gggaagctta acaggataga
      661 tggcgccttg cctatggtta tttccatgcg ggaagaaaat atcaaaagct gtattgtacc
      721 ctatgacaat agagaagaat gtggcttaat aacagacatt gagataatag ctgttaagaa
      781 tttaaaggaa gcagttgcct acctaaacgg cgaaaaagaa atcttaccct acaagggaaa
      841 agaaatccag tgggatattg gagaagaaca taagctagat tattctgata taaggggtca
      901 agaaagccta aaaagggctt tagaaattgc ggctgctgga agccacaatt gtattttagt
      961 aggccctcca ggttctggta aaaccatggc tgccagaagg ctacctacaa tattacctaa
     1021 actttctttt gaagagtcga tagaagttac gaaaatctac agcgtagcag gtttaatgga
     1081 ttctaagggc ttgattatgg aaaggccttt caggtccccc catcatacaa gctccagtgc
     1141 tgcccttata ggaggcggta gaatccctaa accgggag
//
LOCUS       DATGSI010000652         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_233747, whole genome shotgun sequence.
ACCESSION   DATGSI010000652 DATGSI010000000
VERSION     DATGSI010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233747"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VK031_09950"
     CDS             complement(<1..354)
                     /locus_tag="VK031_09950"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004836690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HLS54287.1"
                     /translation="MKKFMDENFLLGNETAEKLYHEYAKDMPIFDYHCHLSPKEIAED
                     KSYRNITEIWLGGDHYKWRAMRSNGIDEKYITGDASDYEKFKAWAGTLPYCLGNPLYH
                     WSHMELQRYFNIDTII"
     gene            complement(407..1900)
                     /locus_tag="VK031_09955"
     CDS             complement(407..1900)
                     /locus_tag="VK031_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HLS54288.1"
                     /translation="MKETILIDKKDNVLVALRDIEAGKLVKDGDKEIELLEDVKAGHK
                     IARRDIKKHENIIRYGYPIGHATEDIKKGRWIHTHNLKTNLSGSLEYEYKPKLVETHK
                     KPGPTTFMGYKRKDGQVGIRNELWIVPTVGCVNGIADLIIKEFENSYDIDYTNIDNIH
                     VYKHSYGCSQLGDDHVNTRTILADIVKHPNAGGVLVLGLGCENNTIDEFKTALGDYDK
                     DRTRFLVTQEVGNEIEKGVELLTQLYESMREDRREELPISELKIGLKCGGSDGFSGIT
                     ANPLLGEFSDYLIAHGGTTILTEVPEMFGAETILMDRAQSQQVFEDTVNLINNFKEYF
                     IEHNQPVYENPSPGNKEGGITTLEEKSLGCTQKGGKSTVVDVLEYGEILEKKGLNLLN
                     APGNDLVASTALGASGCQMVLFTTGRGTPFGSFVPTMKIATNSRIYKLKPHWMDFNAG
                     QLLEGKSMEKLTEEFVEYVVKVASGEYVNNERSGFREIAIFKTGVTL"
     gene            complement(1902..>2862)
                     /locus_tag="VK031_09960"
     CDS             complement(1902..>2862)
                     /locus_tag="VK031_09960"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: protein motif:HMM:NF002969.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HLS54289.1"
                     /translation="LLILPCELIDNNGDTLKEIVLKLAENWGLEEDFAQWVEDANIFY
                     NTLVDRIVTGYPKDNIDDLEKELGYEDDFLVQGEIFHLWVIEGPNQIKEEFPADKLGL
                     NVLFVDDLTPYRERKVRILNGAHTSMVPVSYLYGLETVRESIEDEVVGKFIKSAIYDE
                     IIPTLDLREEELLEFADAVVDRFKNPFINHYLMDISLNSMSKFKTRVLPSLLEYNKRK
                     DTLPEKLVFSLAALIYFYKGERKGERINLSDDKEILTFYKEIWNEYNGTQESIETIVE
                     EVLGLESLWEEDLNNIVGLKEKTVDYLGKIIDKGMEETLKEMV"
BASE COUNT          819 a          633 c          380 g         1030 t
ORIGIN      
        1 tattatggta tcgatattaa agtatctttg tagttccata tgcgaccaat gatatagtgg
       61 attgcctaag cagtaaggaa gtgttcctgc ccatgctttg aatttttcat aatcagaggc
      121 atctcccgtt atatattttt catcaatacc attgctcctc atggccctcc atttatagtg
      181 atctccacct aaccaaattt cagttatatt tctataactt ttgtcctcag caatctcctt
      241 aggacttaag tggcagtgat aatcaaaaat aggcatatct tttgcatatt catgatataa
      301 tttttccgca gtctcgttcc caagcaaaaa attttcatcc ataaactttt tcatccaaat
      361 gacctcccaa tttatattga agataagctt acataaatat atttaattat aaggttactc
      421 cagtcttaaa tatggctatt tctctaaaac cacttctttc attgtttaca tattctccac
      481 tggcaacctt tacaacatat tcaacaaatt cttcagttag tttttccata gacttacctt
      541 ccagcaattg acctgcatta aagtccatcc aatgaggctt taatttataa atcctgctat
      601 tggtagcaat cttcatagtt ggaacaaaac ttccaaaggg tgtccccctt ccagtagtaa
      661 acaaaaccat ttgacaaccg gacgcgccta aggctgtgga agccaccaga tcatttccag
      721 gggcatttag aaggtttaag ccctttttct ctagaatttc tccatactcc agtacatcta
      781 ctactgtaga cttcccacct ttttgtgtac agcctaaaga tttttcttct aaggtagtaa
      841 tgcctccctc cttgttacct ggagaaggat tttcataaac tggctgatta tgctctataa
      901 aatattcttt aaaattatta atcaaattta cagtatcttc aaacacctgc tgtgactggg
      961 ccctatccat taaaatggtc tcagccccaa acatttcagg aacctctgtt aaaatagtag
     1021 ttcccccatg ggctattaag tagtcagaga attctcctaa caggggattg gctgtaatac
     1081 cggaaaagcc gtctgaccca ccacatttaa ggccaatttt taattcagaa ataggaagtt
     1141 cttcccttct atcttccctc atgctttcat ataattgggt taatagctct accccttttt
     1201 caatctcatt gcccacttcc tgtgttacta aaaatctagt tctatcttta tcataatcgc
     1261 ctaaggcagt cttaaattca tcaattgtat tattttcgca accaaggcct aggactaaaa
     1321 ctcctcctgc attagggtgc ttaactatat ctgctagaat agttctagta tttacatgat
     1381 catctcccag ttgggagcaa ccataactat gcttatatac atgaatatta tcaatgtttg
     1441 tataatctat atcataacta ttttcaaatt ccttaattat taagtctgct ataccattta
     1501 cacagcctac tgttggcact atccataatt catttctaat tcctacctgc ccatcttttc
     1561 ttttataacc cataaaggtg gttggacctg gttttttatg ggtttccacc agtttaggct
     1621 tatattcata ctctaaggaa ccagataaat tggtcttcag attatgtgta tggatccacc
     1681 ggcctttttt tatatcctca gtggcatgtc ctataggata gccatatctg ataatgtttt
     1741 catgcttttt gatatctctt ctagcaattt tgtggcctgc cttaacatcc tccagtagtt
     1801 ctatttcttt gtctccatct ttaaccaatt ttccagcctc aatatctctt aaggccacca
     1861 aaacattatc tttcttatca attagaattg tttctttcat attacaccat ttcctttaaa
     1921 gtttcttcca tacccttatc gataatctta cccaaataat ctacagtctt ctcctttaag
     1981 cctacaatat tgtttagatc ttcctcccat agggactcta gacctaaaac ctcttcaact
     2041 atagtttcta ttgattcttg cgttccatta tattcattcc atatttcttt atagaaagtt
     2101 agtatctcct tatcatcaga aagatttatt ctttctccct ttctttcgcc cttatagaaa
     2161 taaatcaagg cagctaggga aaatactaac ttttcaggta atgtatcttt ccttttattg
     2221 tactccaaca gggatggtag aaccctggtt ttaaatttgc tcatagaatt taaagatata
     2281 tccattaaat aatgatttat gaaaggattt ttgaacctgt caacaacggc atctgcaaat
     2341 tctaataatt cttcctccct taagtctaga gttggaataa tttcatcata aattgcagat
     2401 ttaataaact ttcctaccac ttcatcctct atggattctc ttacagtttc aagtccataa
     2461 aggtatgaaa ccggtaccat ggaagtatgg gccccattta aaattctaac ctttctctcc
     2521 ctataagggg ttaagtcatc tacaaatagt acattcagtc ccagtttatc tgcaggaaac
     2581 tcttctttta tttggttagg tccttcaata acccaaagat ggaaaatctc accttgaact
     2641 aaaaagtcat cctcatagcc tagttctttt tctaaatcat ctatattatc ctttggataa
     2701 cctgttacaa ttctatctac taaagtattg tagaagatgt ttgcatcctc tacccactgg
     2761 gcgaagtctt cctccagccc ccaattttca gcaagtttta atactatttc tttcaatgtg
     2821 tctccattat tgtctattag ctcacaaggt aatattaata gt
//
LOCUS       DATGSI010000653         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_140708, whole genome shotgun sequence.
ACCESSION   DATGSI010000653 DATGSI010000000
VERSION     DATGSI010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140708"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VK031_09965"
     CDS             <1..367
                     /locus_tag="VK031_09965"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLS54290.1"
                     /translation="NRISSYYGWRVHPIHNVSRLHTGIDIPAPTGTIVRAAAAGKVMT
                     SGAQGTYGNTVTIDHGGGISTLYAHNSSLLVSAGQTVNAGDPIAKVGSTGLSTGPHCH
                     FEVRKNGTHVDPLPWLRGN"
     gene            467..1690
                     /locus_tag="VK031_09970"
     CDS             467..1690
                     /locus_tag="VK031_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLS54291.1"
                     /translation="MSKRRIVTISIILLIVTNIITFGLTNALTIQLDSRVIIPKKEYD
                     ELYSAYKDYTKVILVESLINEDYLQDVDREQLLEGQLKGMVHALGDPYSQYLTAEEYK
                     DLMEQTSGSFGGIGVIVTPGDDNLITVVSPIEDTPGERAGIRPGDKIIKVDGVEYTGD
                     SMDKAVNVMKGEPDTEVTLTIMRKGKNGQDEMIDLEIVREVIRIVTVKSSIIDDEIGY
                     INLTSFDELTYGDFKKDLKKLQEANVKGLVLDLRNNPGGLLDACADIADEFLGEGVIV
                     YTENKQGERRYYRSKKSHTDIPMVVLVNEGSASASEILAGAIKDHNRGELIGTKTFGK
                     GIVQSIKRLGDGSGLKLTVSEYFTPKGINIHKIGIEPDIVVELPEDIETIGVENIKED
                     TQLQKALEVLKNKIQ"
     gene            1718..>2566
                     /locus_tag="VK031_09975"
     CDS             1718..>2566
                     /locus_tag="VK031_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54292.1"
                     /translation="MYGLYQIIYFTVIDILKTLLSPFFLVIVLIIFFQYKQVGDRPLR
                     ATIESILYGILGGIIATVSFIYLRIYIEPKDFMYILIFAIILSQINARFVCFSYGGSL
                     LVLWHLITGYPKIRPYQIMTVVAILHIVESILIFLNGKAQTSSGYFKLNGKLTAGLLF
                     NRFWPIPFVIFIGDVMIRPITLIGMLNYADFTISDYPRLKLIETGFTLFCYSSLLLII
                     TVSSSNQYLPPIFALVGHEAIIYLSKIREKRRSGSQRPGIRGLRVMQVRKDSVAEEVG
                     IRQGDII"
BASE COUNT          898 a          369 c          538 g          761 t
ORIGIN      
        1 taataggata agttcttatt atggttggag agtgcacccg atacacaatg tatctaggct
       61 acatacaggt atagatatac cagcaccaac aggtaccatt gtgcgagcag cagccgcagg
      121 aaaggttatg accagtggag cccagggaac ctatggtaat acagtaacta ttgaccatgg
      181 tgggggaatt tccaccctct atgcacataa ttcaagtcta ctggtttcgg caggccagac
      241 tgttaatgct ggggatccaa ttgcaaaggt cggtagtacc ggattgtcaa caggaccaca
      301 ttgtcacttt gaagttagga aaaatggtac tcacgtagat ccgttaccat ggctaagggg
      361 gaattaattt cccctttttt tattttattt aaatccatag aatggtataa tgttgggaga
      421 ggtttaataa aataacatag tagtctaaaa tatatgaggt gaaaagatgt ctaaacgtag
      481 aatagtaaca atatctataa tattattgat agttactaat ataataacat tcggtttaac
      541 aaatgcgttg actatccaat tagattctag ggtaataatt cccaagaagg aatatgatga
      601 gttatattct gcctataagg attatactaa ggtaatatta gttgaatcct taatcaatga
      661 agattatttg caagatgtgg atagagaaca attgttagaa gggcagctaa aggggatggt
      721 ccatgcccta ggcgatcctt attcccaata tttaactgca gaagaataca aggatcttat
      781 ggaacaaaca tcaggatcct ttggtggtat tggggtgata gtaactcctg gtgatgataa
      841 tctaattaca gttgtatctc ccattgaaga tactccagga gagagagctg gaataaggcc
      901 aggtgacaaa ataattaagg tggatggcgt agagtatact ggagatagca tggataaggc
      961 tgtaaatgtt atgaaaggtg agcctgacac tgaagttacc ctaaccataa tgcgtaaggg
     1021 gaaaaatgga caggatgaaa tgatagacct agaaatagta agagaagtca taagaatagt
     1081 tactgtaaag tccagtatta ttgacgatga gataggatat ataaatctaa cctcctttga
     1141 tgagttaaca tatggagact ttaaaaagga tctgaaaaaa ttacaagagg ccaatgtaaa
     1201 ggggcttgtc ttagatttaa gaaataatcc aggtggacta ttagatgctt gtgctgatat
     1261 agcagatgaa tttttaggag aaggggtaat agtatataca gaaaacaagc agggagaaag
     1321 aagatattat agatctaaaa agtcacatac agacataccc atggttgttt tagtcaatga
     1381 gggcagtgcc agtgcttcag aaattttagc tggagccatt aaagatcaca acagaggaga
     1441 attaataggc actaaaacct ttggaaaagg aatagttcaa agtataaaaa gactaggaga
     1501 tggttcagga ttaaaattaa cagtatcaga atattttact cctaaaggaa ttaatatcca
     1561 taaaataggg attgaaccag atattgttgt tgaattgcca gaagatatag aaacaattgg
     1621 agttgaaaac attaaggaag atacccagct acagaaggcc ttagaggtat taaaaaataa
     1681 aattcaatag tctgaaagta aaaaagggga aacttaaatg tacgggctat atcaaataat
     1741 ttatttcaca gttattgata ttcttaaaac attgttatct cctttttttc ttgtaatagt
     1801 cctaatcatt ttttttcaat acaagcaggt aggagataga cccttaagag ctaccataga
     1861 atcaattctt tatgggattt taggtggaat tattgccaca gtttcattta tttatctgag
     1921 aatatatata gagcctaaag actttatgta tattttaata tttgctatca ttttgtccca
     1981 aataaatgct agatttgtct gtttctccta tggaggtagc ttattagttt tgtggcattt
     2041 aattacaggc tatcctaaaa taaggcccta ccagattatg acagttgtag ccatactgca
     2101 tatagtagaa agtattttaa tattcttaaa tggaaaggcc cagacttcat caggatattt
     2161 taagctaaat ggaaaactta cagctgggct tttatttaat agattttggc caattccctt
     2221 tgttatcttt ataggtgatg tgatgatcag gccaataacc ttaataggga tgcttaacta
     2281 tgcagatttt actatctctg attatcctag gttaaaatta atagaaacag gatttaccct
     2341 attttgctat agtagcctct tattaataat cactgtttct tcttccaatc aatacctgcc
     2401 cccaatcttt gccctagtag gccatgaagc aataatttat ctaagcaaga ttagagaaaa
     2461 aagacggagt ggatcacaaa ggcctggtat aaggggatta agggtgatgc aagtaagaaa
     2521 agacagtgtg gctgaggaag taggaataag gcaaggggat attatt
//
LOCUS       DATGSI010000654         6591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_658281, whole genome shotgun sequence.
ACCESSION   DATGSI010000654 DATGSI010000000
VERSION     DATGSI010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658281"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="VK031_09980"
     CDS             complement(<1..1390)
                     /locus_tag="VK031_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS54293.1"
                     /translation="MGKLLPYMKKYRLYAILTPILMILEVLGDIMLPYLMSLIVDIGI
                     KNKDTQYVIKIGVVMIIATLLSMLFGIISAHFGARAGHGFASEIRKETFKRIQGFSFA
                     NLNDFTVSSLVTRLTTDCNIIGRVTMMSLRLAVRAPFMMIFALIMATRINPSLVRIFL
                     VSIPITVGIISVVIWLARPLFLKLQTKVDDVNAVIQENLTAIRVVKSFNREEHEEKKF
                     KKRNDSLKDTALEALSLVIFIMPVLNLVIFSTIIAILWFGGMQVRMGTMEGGQLIAFI
                     TYVTQILITLMMISMFFMQLLRGITSAERIIEVWDKTSEIVEIDNPVKEVKNGSVEFN
                     NVSFCYPNSKEKTLRNINFRIEAGEVLGIIGSTGSSKSTLVQLIPRLYDVTEGSVKVG
                     EVDVRDYDIELLRDEVAFVLQKNTLFSGTIRENMLWGNENATDEEIIQALKHAQAWEF
                     VSKYDDQLDHRVE"
     gene            complement(1415..1855)
                     /locus_tag="VK031_09985"
     CDS             complement(1415..1855)
                     /locus_tag="VK031_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLS54294.1"
                     /translation="MLKTDVALQLRATHHKMIQLMKAKISEYDLTFGLLNILVRIEKN
                     PNTNQKQLAEDMNFTQGAMSIAVKRLIENDMIKQVPLESDMRYNRLNLTKKGKAMIAS
                     CKDHMTERYENIFYGFDDGELTQLYNFLKKINNNLDKINKEDCL"
     gene            complement(1970..3061)
                     /gene="ychF"
                     /locus_tag="VK031_09990"
     CDS             complement(1970..3061)
                     /gene="ychF"
                     /locus_tag="VK031_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HLS54295.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDDRLDVLAKIYPSKRTVPTAIEFCDIAGLVKGASQGEGLGNKFLSHIREVDAIVHVV
                     RCFQDEDITHVDGSVDPVRDIEVINLELMLSDMELLQRRLDRTQRAAKGDKGLVAEVK
                     LIERILNTLEEGGSIRGLDLAEEENKILRSFNLLSNKPVIYVANISEDDLEAGAKNDY
                     VKQVVKYAAGEDAEVVVISAAIEEEISQLADEEKLAFLQDLGLERSGLDQLIVASYKL
                     LGLISFLTAGPQEARAWTIKEGTAAPQAAGKIHSDMERGFIRAEVISFEDLVESGSMV
                     AAREKGLVRIEGKDYIVQDGDVVLFRFNV"
     gene            3264..4151
                     /locus_tag="VK031_09995"
     CDS             3264..4151
                     /locus_tag="VK031_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLS54296.1"
                     /translation="MTDKKDNSKLGCEVLQPFNERKPLKDIERSIIKTYRKHIWRKFV
                     KAIREYKLIEDGDRIAVAISGGKDSLLMAKLFQELHRYGNKNFDVEYIAMDPGYHPEI
                     RELLEENCQYLDIPVQIFETDVFQVADKIAGDYPCYMCARMRRGALYGKAEELACNKL
                     ALGHHFNDVIETIMLNILCSGNFKTMMPKLKAKNFENMELIRPLYLIEEEYIKRFIQN
                     SGIMPLNCACMVAAKKIGNKRFEIKDLIENLKKNFQNVDMSIFRAAENVNMEAIIGWE
                     KDGKKYSFLDYYDEGDEDK"
     gene            4283..4951
                     /locus_tag="VK031_10000"
     CDS             4283..4951
                     /locus_tag="VK031_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54297.1"
                     /translation="MGKIIKRLLRLFLGLFLFALGAVTIINAKIGVAPWDVFHQGLSN
                     VTSLTVGRANIYTGLIFVILNTILGQRLGWGTVLNMIFIGVFIDILMLNNLVPMPGNL
                     LIRYMMLFLGIIAQGLGTYFYISTSLGIGPRDGLMVILMKKTGYSVGIIKNIMEISAV
                     TIGFFLGGNFGIGTLIMAFVAGHIWDYLFKRLNFVVDDVEHRAIKDDYEYFLKRLKKE
                     EDAN"
     gene            complement(4944..6296)
                     /locus_tag="VK031_10005"
     CDS             complement(4944..6296)
                     /locus_tag="VK031_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLS54298.1"
                     /translation="MKNYPLSVQIWIVFALITLAITTLLSLIFPLTLRLFFTNEIYST
                     IESAQNILLNRYPIEDFWNEPAIDGNEGLDDIRTVNHFLVYGDNQILLNRPVSFDLIR
                     QVRKDISQQENVRQRYSGKYSEGKLFYIITKVRSFGRDTYLVSYMGDSYRKDLVKTLF
                     YRLISIMVLIIILSWIPALLLSRYLSSPLVNLESRVDRLAKRDWREPINLGRKDEIGK
                     LGNSVEKLRQELIRQDEAERTFLQNISHELKTPVMIIRSFAQAIKDGIFPKGNLDNSV
                     EVIDEEAERLEKKIENLLYFSKLHYMFNHEMKVKSFPLDSLIVNVIDRFLWNLQDIQL
                     ETYLEPLDIVGDEDQWRVVIENILDNQIRYAKEKISISLKQLEDHILLEIWNDGPHIE
                     TETMENLFTEYNKGYSGKFGLGLAIVYRILNNHNSSIQAINEDQGVKFIIKIKEEDAN
                     "
     gene            complement(6293..>6591)
                     /locus_tag="VK031_10010"
     CDS             complement(6293..>6591)
                     /locus_tag="VK031_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLS54299.1"
                     /translation="KPTDLIKIGPYTINTGSRIVSLDGKDIDLTSKEYELLSYFATNL
                     GLALSREQILDQIWGSDYFGSDRVVDDLVRRLRKKMPKLNIETVYGYGYRVNQS"
BASE COUNT         2097 a         1237 c         1079 g         2178 t
ORIGIN      
        1 gctctaccct gtgatccaac tgatcatcat acttggaaac aaattcccaa gcttgagcgt
       61 gctttaaggc ttgaataatt tcttcatctg ttgcattttc attaccccaa agcatattct
      121 ctctaatggt accagaaaaa agagtgtttt tctgtaaaac gaaggctact tcatctctta
      181 acagctcaat atcgtaatcc cttacatcta cttctccaac ctttacagaa ccttctgtta
      241 catcatagag cctagggatt agttgaacta aagtagattt ggatgaaccg gtagaaccta
      301 taatacctag aacctcacct gcttctatcc taaagtttat atttcttaag gtcttctcct
      361 tactattggg atagcagaag gacacattat taaattctac agagccgttt ttcacttctt
      421 ttacaggatt atcaatttcc acaatttcag aagtcttatc ccatacttct attattcttt
      481 ctgcagaggt tatacccctt aacagttgca taaagaacat ggaaatcatc atcagggtga
      541 tgagaatctg ggttacatag gtaataaagg caattaactg accgccctcc atggtcccca
      601 tccttacctg cattccacca aaccaaagaa tagctattat ggtggagaat ataaccagat
      661 ttagcacagg cataataaat atgactaaag aaagagcctc taaggcagta tcctttaagg
      721 aatcatttct ctttttaaat tttttctctt catgctcttc ccggttaaag gacttaacaa
      781 ccctaatggc agttaagttt tcttgaatta ctgcgttaac atcgtcaacc ttagtttgta
      841 actttaaaaa caaaggcctg gctagccata taacaacaga tataatacct actgtaatag
      901 gtatagatac tagaaatatt ctaactaaag atggattaat tctagttgcc ataatcaagg
      961 caaaaatcat cataaaagga gctctaactg ccagccttaa actcatcatg gttaccctac
     1021 ctataatatt acagtctgtt gtaagcctgg taaccaagga tgaaactgta aagtcgttaa
     1081 gattggcaaa agaaaatcct tgaatcctct taaaggtctc ttttctaatt tcagatgcaa
     1141 atccatgtcc tgctctggcc ccaaaatggg cacttattat accaaatagc atactaagca
     1201 gggttgctat aatcataact accccgattt taattacata ctgggtatcc ttatttttta
     1261 tccctatgtc aacaataagt gacataaggt aaggtaacat tatgtcacct aatacttcta
     1321 aaatcataag tataggtgtt aaaattgcat agagcctata ttttttcata tagggtagta
     1381 atttccccaa aatatccctc ctgttctgga aaatttataa gcaatcttcc ttattaatct
     1441 tgtctaaatt attattaatc ttttttaaaa aattatataa ctgggttaac tcaccatcat
     1501 cgaatccata aaagatattt tcatatcttt cagtcatatg atctttacaa ctggctatca
     1561 tagctttacc ctttttagtt aggtttaacc tgttatatct catatctgat tctaagggaa
     1621 cttgtttgat catatcattt tctattagcc ttttaacggc tatactcatg gcaccctggg
     1681 tgaaattcat atcttctgcc agttgttttt ggttggtatt aggatttttt tctatcctta
     1741 ctaatatatt aagtaggcca aaggtcaggt catactcact aatcttggcc ttcattaact
     1801 gtatcatctt atgatgggta gccctaagct gtagagccac atcagttttc agcatatgat
     1861 caccccaata gcttgcttta agtaataata ctttaatact aaactaattt ataaatactg
     1921 tcaataaaaa ttaatttatt aacaaaaaag actaagcatt gcttagtctt tacacattaa
     1981 atctaaataa caccacatct ccgtcttgga caatataatc cttaccttca atccttacca
     2041 gccccttttc cctggcggct accatactgc ctgattcaac taaatcctca aaggaaataa
     2101 cttcagccct tataaaacct ctttccatat cagaatggat ttttcctgcc gcctgcggag
     2161 cagctgtacc ttccttaatg gtccaggctc tagcctcctg gggaccagca gttaaaaagc
     2221 taattaaacc taataatttg tagctggcca caattaactg atcaaggcca gacctttcta
     2281 aacctagatc ttgtagaaag gctagctttt cctcatctgc caattgagaa atttcttcct
     2341 ctattgctgc actgataact accacctcag catcttctcc agctgcatat tttacaactt
     2401 gttttacata atcattcttg gctccagctt ccaaatcatc ctctgatata ttggccacat
     2461 aaattacagg cttgttactt agaaggttga aggatcttaa aattttattt tcctcttctg
     2521 ctaaatccag tccccttata gagccacctt cttctaaggt atttaatatt ctttcaataa
     2581 gtttaacctc agccactaaa cctttatctc ccttggctgc cctttgggtc ctatctaatc
     2641 ttctttgtaa aagttccatg tctgatagca ttaattctag attaataact tcaatatccc
     2701 taacagggtc cactgaacca tcaacatggg taatatcttc atcttgaaag catctaacta
     2761 catggactat ggcatctacc tccctaatat gagatagaaa cttattgcct aaaccttccc
     2821 cttggctagc cccttttacc aagcctgcaa tatcgcaaaa ttctatggct gttgggactg
     2881 ttctttttga aggataaatt ttagccagta catctaacct atcatctgga actgatacaa
     2941 ctcccacatt aggatctatg gtacaaaatg gataattagc cgactctgca ccagcttgag
     3001 ttatggcatt aaataaggta ctttttccta catttggtag gcctacaatt cccagtttca
     3061 tctaaaatgt cctttctata ttaataattc ttgtaataat tatacttaga aatcttcaca
     3121 aaattatatt atactataga tgactataac aagcaaagaa atagtaagag attcttaaga
     3181 agtccaaaat atatctcatt tagtttttat attaattata gtatactata taatgattac
     3241 aaactttagc tggagtgata ctaatgacag ataaaaagga taactctaaa ttagggtgtg
     3301 aagttctaca accctttaat gaaagaaagc ctttaaagga tatagaaaga agcattatta
     3361 agacctatag gaaacacatt tggagaaaat ttgtaaaagc aatcagagaa tataaattaa
     3421 tagaggatgg ggacaggata gctgttgcca tatctggagg gaaggatagc ctgcttatgg
     3481 ccaagctttt ccaagagcta catagatatg gcaataaaaa ttttgatgta gagtatattg
     3541 ccatggatcc aggctatcat cctgagataa gggagctttt ggaggaaaac tgccaatatt
     3601 tagatatacc agtccaaatc tttgagacag atgtttttca agtagcagat aaaatagcag
     3661 gggattatcc ctgttatatg tgtgccagaa tgagaagggg agccttatat ggaaaagcgg
     3721 aagaactagc ttgtaataag ctggccctag gccatcattt taacgatgtc attgagacta
     3781 ttatgcttaa tatattatgt tctggcaatt ttaaaaccat gatgcctaaa ttaaaggcta
     3841 aaaactttga aaatatggag ctaattaggc ccttatatct aatagaagaa gaatatatta
     3901 aaaggtttat tcaaaatagt ggtattatgc ccctaaattg tgcctgtatg gtggcagcca
     3961 aaaaaattgg taacaaaaga tttgaaataa aggatttaat agagaattta aagaaaaact
     4021 ttcaaaatgt ggatatgtca atttttaggg cagctgaaaa tgttaatatg gaggcaatta
     4081 taggttggga aaaggatggg aaaaagtact ccttcctaga ttattatgat gagggagatg
     4141 aagataaata aattatggaa gtttaacaat ttctatgtaa aaaagtactt ctttggtata
     4201 gaatggatat ataataaact ttaaatgggg gataggacag tgaatccttg atgtttcctt
     4261 gcaggggagt agctgaaaag atatgggaaa aattataaaa aggcttctta gattgttttt
     4321 aggacttttc ttgtttgcct taggtgcagt gactattata aatgctaaaa ttggagtagc
     4381 tccctgggat gtgtttcatc agggcctctc taatgtaacc agcttaacag tcggtagggc
     4441 aaatatttac acaggcttaa tatttgttat tttaaatacc atactaggtc aaagattggg
     4501 ctgggggaca gtgttaaata tgatctttat aggtgtcttt atagatattt taatgctaaa
     4561 taatctggtt cctatgcctg gcaatctact aataagatat atgatgctct ttttaggcat
     4621 aattgctcag gggcttggaa cctactttta tatatctact agcctaggaa ttgggcctcg
     4681 ggatggcctg atggttatac taatgaaaaa aacaggctac tctgtaggta taattaaaaa
     4741 tattatggaa attagtgctg taactatagg cttttttttg ggaggaaatt ttggaattgg
     4801 gactttaatt atggcctttg tggcaggcca tatttgggat tatcttttta agaggcttaa
     4861 ctttgtggta gatgatgtag aacatagggc gattaaggat gattatgaat attttctaaa
     4921 aagacttaaa aaagaagagg atgctaatta gcatcctctt cttttatttt aattatgaat
     4981 ttgacacctt gatcttcatt tatagcctga atactagaat tatggttatt taaaatccta
     5041 taaactatgg ctaggcctag accaaatttt ccgctatacc ccttattata ttcagtaaag
     5101 agattctcca tggtttcagt ctctatatgg ggcccgtcat tccatatttc taataggata
     5161 tgatcctcca gctgcttaag gctgatggaa attttttctt ttgcatacct gatttgattg
     5221 tctaatatat tttctataac caccctccac tgatcctcat ctcccacaat atctaaaggt
     5281 tccagataag tttccagctg aatgtcttgc aaattccaca aaaatctgtc aataacgttt
     5341 acaattaagg aatcaagagg aaaactttta actttcattt catgattaaa catataatgt
     5401 agtttggaaa aatacaaaag attttctatc tttttttcca atctttcagc ttcctcatct
     5461 ataacttcca ccgaattatc caaattccct ttagggaaaa taccatcctt tatggcctgg
     5521 gcaaagcttc tgattatcat aactggagtc tttagctcat gggaaatatt ttgcaaaaag
     5581 gtcctttctg cttcatcctg ccttataagc tcttgcctaa gtttttctac agagttgcct
     5641 agctttccaa tctcatcctt tcgacctaga ttgataggtt cccgccaatc ccttttagcc
     5701 agcctatcta cccgtgattc cagattgacc agagggctag aaaggtatct agataaaagt
     5761 agggccggta tccagcttaa aatgataata agaaccatta tggatattag acgataaaac
     5821 aaggttttaa ccaggtcctt cctataggaa tcacccatat aagagaccag gtaggtatct
     5881 ctaccaaagc ttcttacctt ggtaattata taaaatagtt ttccttcact atattttcca
     5941 ctatagcgct gccttacatt ttcctgttga ctaatatctt ttctaacctg ccttattaaa
     6001 tcaaaggaaa caggcctgtt taaaaggatc tgattatctc catatacaag aaagtggtta
     6061 acagtcctaa tatcatccag cccttcattc ccatcaatgg caggttcatt ccaaaaatct
     6121 tcaatagggt acctattaag taagatattt tgggctgact ctattgtaga ataaatttca
     6181 ttggtaaaaa acaagcgtag agttaagggg aaaattaggg ataataaggt ggtaatagct
     6241 aaagttataa gggcaaagac aatccatatt tgtacactta aagggtaatt tttcatgatt
     6301 gattcaccct atatccatag ccataaactg tttctatatt aagctttggc atcttttttc
     6361 tcagccgtct aaccagatca tctactactc tatctgaacc gaaatagtcg ctaccccata
     6421 tttggtctaa gatttgttct ctagacaggg ctaagccaag attggtggca aagtatgata
     6481 ataactcata ttccttggaa gttaagtcaa tatctttacc atcaagactg actattcgac
     6541 tacctgtatt gattgtatag gggcctatct ttataagatc tgtaggcttt t
//
LOCUS       DATGSI010000655         1829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_275708, whole genome shotgun sequence.
ACCESSION   DATGSI010000655 DATGSI010000000
VERSION     DATGSI010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275708"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VK031_10015"
     CDS             <1..884
                     /locus_tag="VK031_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HLS54300.1"
                     /translation="NELQKLPVYDIVLGSIGLIVGLLIAYLLGKPLDNLKIPYLGFIA
                     QLILLGIFGYLGVKIPTRKRDDITTAIGDMNINKKFVKDKLKDKYKSCPKILDTSVII
                     DGRIADICKTGFIEGPLIIPEFVLEELQHIADSSDSLKRNRGRRGLDILNKIQKELDI
                     EIIIHDKKFQDIEEVDSKLLKLTQMLEGKIITNDYNLNKVAGVQGIQVLNINELANAI
                     KPVVLPGEEMVVHIVKDGKETGQGLAYLDDGTMIVVESGKKYIGETVDVVVTSVLQTS
                     AGRMIFAKPKALVDKAV"
     gene            1056..1799
                     /gene="ispD"
                     /locus_tag="VK031_10020"
     CDS             1056..1799
                     /gene="ispD"
                     /locus_tag="VK031_10020"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HLS54301.1"
                     /translation="MYKNYHVSAIIAAAGMSNRMGSKINKQFIAIDNKPILVHTLEKF
                     EKCKYIDEIILVSKEEEVEYCRKEIVKKYGFKKVVKIIRGGKERQDSIYNGLLALNEN
                     TDIVLTHDGARPFVKIKSIEAGIKGVVEHGACVIGVPVKDTIKAVDEKELIHHTPKRS
                     LLWAAQTPQCFWTSLIKQGYEHAIKEGIVGTDDSSLVEKIGHKVKMIMGSYDNIKITT
                     PEDLVIGEFLLKSKENILQSRAYLFKVDR"
BASE COUNT          695 a          213 c          390 g          531 t
ORIGIN      
        1 aaaatgagtt acagaaatta cctgtttatg atattgtttt agggtctatc ggcctcatag
       61 taggtttatt aatagcctat ttactaggca agcctttaga taatttaaaa attccctatt
      121 tagggtttat agctcagtta attttactag gtatttttgg ttatttaggt gtaaagatcc
      181 ctactagaaa gagggatgat attacaactg ccattggaga tatgaatata aacaagaagt
      241 ttgttaagga taagctaaaa gacaagtata aatcatgtcc caagatatta gatacctctg
      301 tcataataga tggaagaata gctgatatat gtaagacagg ttttattgag gggcctttga
      361 ttataccgga atttgtgtta gaagagttgc agcatatagc agattcttca gacagcttga
      421 agcgcaatag aggtagacga ggtttagata tacttaataa aattcagaag gaattggata
      481 ttgaaataat aattcatgac aaaaaatttc aagatataga agaagtggat tcaaagttat
      541 taaaattaac ccaaatgtta gaagggaaaa ttatcaccaa cgattataac ttaaataaag
      601 tagcgggagt gcaaggaatt caagtcttaa acattaatga attggctaat gctattaagc
      661 ctgtagttct acctggagag gaaatggtag ttcatatagt taaagatgga aaagaaactg
      721 gccagggatt ggcttattta gatgatggaa ccatgatagt ggttgaatca ggcaaaaaat
      781 atataggaga aacagttgat gtggttgtaa cttctgtact ccagacttca gctggaagaa
      841 tgatttttgc aaagccaaag gcactggtag acaaggcagt ataaggaccc ggaaagggtc
      901 ttttttattg ggaaaattac tgtttttatt gaaagatttt atgaaaacaa agtgtattat
      961 tttgcctatc tattaaagat ataatatata aattataaaa tatgctataa tataagataa
     1021 ttaagtttta caaatgacag attagaggtg aaataatgta caaaaattat catgtgtctg
     1081 ccataatagc ggcagcaggc atgagtaata gaatgggaag caaaataaat aaacaattta
     1141 ttgctataga taacaagcct atattagtac atacattgga aaaatttgaa aaatgtaaat
     1201 atattgatga aatcatccta gtttccaaag aggaagaggt ggagtactgt agaaaggaaa
     1261 tagtaaaaaa atatggtttt aaaaaggtag ttaaaatcat aagaggggga aaagaaagac
     1321 aggactcaat atataacgga ttacttgccc ttaatgaaaa tacggatata gtccttaccc
     1381 atgatggggc caggcccttt gtaaaaataa aaagtataga ggctggaata aagggagtag
     1441 tagaacatgg tgcctgtgtt ataggtgtgc cagttaagga tacaataaag gcagtagatg
     1501 aaaaggaact tatccatcat acgcctaaac gaagcctttt atgggctgca caaactccac
     1561 aatgcttctg gactagtctt attaagcagg gttatgaaca tgccatcaag gaaggcatag
     1621 taggaacaga cgatagttct ttggttgaga aaataggtca caaggttaaa atgataatgg
     1681 gaagttacga taacataaaa ataactactc cagaagattt ggtaattgga gagtttttac
     1741 ttaagagtaa ggaaaatatt ttacaaagca gggcctattt atttaaggtg gacaggtgat
     1801 aaaatgagaa ttggatttgg atatgatat
//
LOCUS       DATGSI010000656         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_140935, whole genome shotgun sequence.
ACCESSION   DATGSI010000656 DATGSI010000000
VERSION     DATGSI010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140935"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VK031_10025"
     CDS             complement(<1..406)
                     /locus_tag="VK031_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HLS54302.1"
                     /translation="MKITFPHLGNVYIAGKAFLEELGHTVIPPPVCSRKTLEIGTKLS
                     PEMICLPFKIFMGNYMESIEKGANTILLTGSCGPCRFGFYSLMQRDLLKDLGYKVDMI
                     VLESPREGLDEFRNNVGKLIHSNSLIKIARNIK"
     gene            complement(378..1343)
                     /locus_tag="VK031_10030"
     CDS             complement(378..1343)
                     /locus_tag="VK031_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HLS54303.1"
                     /translation="MKKKTIGIPRGMLYYEFYPMWERYFRSLNFNIISSPRTNKNILD
                     KGILSCVDEACLPVKIFHGHVEYLKDKVDYIFIPKIMSLYKREYCCPKILGLAEMIEN
                     SIKDCPEIIDPIFDYKGRTAFKKAYMETGAQLRVAKKASKKAYKASQIDKNIYRSWLK
                     KEVLKDASLKQENARFNLLILGHSYNVYDEFINMGIIEKLIKEKVNISFAEDVAERDI
                     RSFSNQVRKRIFWTHGRRTVGAALSLIDRHKIDGIIYLNSFGCGLDSVLTHIVARKAK
                     ENNLPTMELTLDEQTGEAGFNTRLEALLDMMKWRDRHEDNIPSFR"
     gene            1444..>2100
                     /locus_tag="VK031_10035"
     CDS             1444..>2100
                     /locus_tag="VK031_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HLS54304.1"
                     /translation="MQVIEREIEYGETLDELLKDNVCFFDIETTGLNRRKNLIYLVGI
                     LYYDEGSNRLKLLQIFAESLQEEPQLLNKAYKLLSSFDKIINYNGNAFDIPFFNQRLK
                     VNGIESSIPREKSLDIYAYLRANRYVLDLDNLRLKTIEEYLGIERKDIYTGRDCIEFY
                     KRYIETGQADLKEKILQHNYDDLYYLPRVMEILTIVRDKKSFLVKEKFKFQINEIKKE
                     G"
BASE COUNT          663 a          373 c          338 g          726 t
ORIGIN      
        1 gttttatatt tctggctatt tttattaaag aatttgaatg aattagcttg cccacattgt
       61 ttctaaattc atctaatcct tctctaggtg attctaatac tatcatgtcc accttgtatc
      121 ctaagtcttt taatagatcc ctttgcatta gggagtaaaa tccaaaccta caggggccac
      181 agctaccagt tagcaggatg gtattggccc ccttctcaat actttccata tagttcccca
      241 taaatatttt gaagggtaaa caaatcatct ctggtgaaag tttggttcca atttctaaag
      301 tttttctgct acagactggc ggtggtatta ctgtatggcc taattcttct aaaaaggcct
      361 tgccagcaat atatacatta cctaaatgag ggaatgttat cttcatgtct atctctccat
      421 ttcatcatat ctaacagggc ctctagtcta gtattaaagc ctgcttctcc tgtttgttca
      481 tctaaggtta gctccatggt gggcagattg ttttccttgg cctttctggc cactatatgg
      541 gtcagtactg agtctaggcc acatccaaag gagttaagat aaataattcc atctatctta
      601 tgcctatcta ttaggctaag ggcagcacct actgtccttc tgccatgggt ccaaaagatc
      661 ctttttctaa cctggttaga aaaacttctt atatcccttt ccgccacatc ctctgcaaag
      721 ctaatattaa ccttctcttt aattagcttt tctataatcc ccatatttat aaattcatca
      781 tagacattat aactatgtcc taagattaga agattaaatc ttgcattttc ttgctttagg
      841 gaagcatctt ttaagacttc tttttttaac caactcctat aaatattttt atctatttgg
      901 ctggccttat aagccttttt acttgccttt tttgccaccc ttaactgtgc tcctgtttcc
      961 atataagcct ttttaaaggc tgttctacct ttgtagtcaa agatagggtc aattatttca
     1021 gggcaatcct ttatactgtt ttcaatcatc tctgccagac ctaatatttt gggacaacaa
     1081 tattcccttt tatataggct cataatcttt ggaataaata tataatcaac cttgtctttt
     1141 aaatattcca catggccatg aaagattttt actggtaaac aggcctcatc tacacaagac
     1201 aggataccct tgtctaaaat atttttatta gtcctagggg aagatataat attaaaatta
     1261 agactacgaa aatatctttc ccacatggga taaaattcgt agtacaacat ccccctaggt
     1321 atgccaatgg ttttcttttt catataatac ctcctccaca ccaatctatt attaacattt
     1381 agccagatta atataactgt acaaatatta aataatagga taatattatg ctgaggtggt
     1441 tttatgcagg ttatagaaag agaaatagaa tatggagaaa ctttagatga actcttaaag
     1501 gataatgtct gttttttcga catagaaacg acaggtctca atagaaggaa aaatcttata
     1561 tatttggtag gtatcctata ttatgatgaa ggaagtaata ggctaaagtt acttcaaatc
     1621 tttgccgaaa gcttacaaga ggagccgcaa cttttaaata aggcctataa acttttgtct
     1681 tcctttgata agattattaa ctacaatggt aatgcttttg acattccctt tttcaaccaa
     1741 aggttaaaag taaatggtat agaaagctct attcctaggg aaaaatcctt ggatatttat
     1801 gcatacctta gggccaatag atatgtttta gacttggata atttaaggct gaaaactata
     1861 gaagaatatt taggtattga aaggaaggat atatacacag gcagggactg tattgagttt
     1921 tataaaagat acatagaaac aggacaggct gatttgaaag aaaaaattct acaacacaac
     1981 tatgatgatc tatattattt gcctagggtt atggagattt taacaatagt aagggataaa
     2041 aaaagttttt tagttaaaga gaaatttaag tttcaaatta atgagattaa aaaggagggg
//
LOCUS       DATGSI010000657         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_91249, whole genome shotgun sequence.
ACCESSION   DATGSI010000657 DATGSI010000000
VERSION     DATGSI010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91249"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /gene="tilS"
                     /locus_tag="VK031_10040"
     CDS             <1..467
                     /gene="tilS"
                     /locus_tag="VK031_10040"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HLS54305.1"
                     /translation="YKKLIFSRNKAVKNINFLYNIKLGVNEFPKIGYRFDIEVLPVEK
                     LGTIKRDKTVGLFDYDKIQGNLYLRNRKVGDRFVPFGMRGSKKIKDYFIDEKIPRQLR
                     EKIPLIVDNKNILWVVGYRTSNLYKITKDTKKILKIQFMRHRIEEDKNGKGH"
     gene            451..987
                     /gene="hpt"
                     /locus_tag="VK031_10045"
     CDS             451..987
                     /gene="hpt"
                     /locus_tag="VK031_10045"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HLS54306.1"
                     /translation="MERAIKKVLIDEETIQNRIKELGEQITKDYKGKDLLVVGILKGA
                     VVFMSELCKHIKLPLKIDFMAVSSYGESATSTGEVKIIKDLDFSVENKDILVVEDIID
                     TGLTLAYLTDNLKKRGANSVKIATLLDKVDRRNIEVKVDYLGFEIPDEFIVGYGLDYA
                     EQYRNFPFVAALKEEIYQ"
     gene            1066..>2562
                     /gene="ftsH"
                     /locus_tag="VK031_10050"
     CDS             1066..>2562
                     /gene="ftsH"
                     /locus_tag="VK031_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HLS54307.1"
                     /translation="MKRFFRGMSIYLLIIILIMFGVQMMGRNVETVKKLDTTELIKNI
                     KDDNIKSVREVGSTIEGKLKDGTQFSTYLPLSLRYNFYQDYLQDLVDSGKISYMGEPE
                     PGKPWYYDLLPTFLMLFVFGVFWFMLMQQTQGGGGGRVMSFGKSRARLHREDEGKLVT
                     FADVAGLHEEKEELQEIVDFLGNPRKYIDIGARIPTGVLLIGPPGTGKTYLSKAVAGE
                     AGVPFFTISGSDSVEMFVGVGASRVRDLFEQAKKNSPCIIFIDEIDAVGRHRGAGMGG
                     GHDEREQTLNQLLVEMDGFGTNEGIIVMAATNRPDILDPALLRPGRFDRQIYVGLPDI
                     RGREEILKVHTKNKPLASDVDLKVVARRTPGFTPADLENLVNEAALLSARYNHKEIPM
                     HLIEEASIKVQAGPEKKSRVVSEKERRLTAYHEAGHALTARLIPGMDPVHMVTIIPRG
                     MAGGFTAYIPEEDVNYRTKSEMEDRLVTLLGGRVSEALVLGDISTGAQN"
BASE COUNT          900 a          335 c          601 g          726 t
ORIGIN      
        1 gctataaaaa attgatcttt agtagaaata aggctgtaaa aaatataaat ttcctatata
       61 atattaagtt gggagtaaat gagtttccta aaataggtta tagatttgat atagaagttt
      121 tacctgtgga aaagttgggt actattaaaa gggataaaac tgtgggtctg tttgactatg
      181 ataagattca gggaaactta tatttaagga atagaaaggt tggagataga ttcgttccct
      241 tcggtatgag gggaagcaaa aaaataaaag attactttat agatgaaaag attcctaggc
      301 aacttagaga aaaaataccc ttaatcgtag acaataaaaa tattttgtgg gtagtaggat
      361 ataggaccag taatctatat aagataacaa aagatacaaa aaagatttta aagattcaat
      421 ttatgcggca tagaattgag gaggacaaaa atggaaaggg ccattaaaaa agtattaatt
      481 gatgaagaga caattcaaaa tagaataaaa gaattaggag aacaaataac taaagattat
      541 aaaggcaaag atctactggt agttggtata ttaaagggtg ctgtagtatt tatgtctgaa
      601 ttgtgcaagc atataaaatt acctttaaag attgacttta tggcagtgtc cagctatggt
      661 gaatctgcca cttctactgg tgaggttaaa ataataaaag atctagattt ttctgtggaa
      721 aataaagata ttctagtggt agaagacatt attgataccg gtcttaccct agcatattta
      781 acagataatt taaagaaaag aggagccaat agtgtaaaaa tagctactct attggataag
      841 gtagatagaa gaaacattga agttaaagta gactatcttg gatttgagat tccagatgaa
      901 tttattgtag gctatggatt ggactatgct gagcagtata ggaactttcc atttgtagcg
      961 gcattaaagg aggaaatcta ccagtagttt ttttattgta gtgtaatatt tattgtggta
     1021 taattaaaag atattatcta cccatatgtg gagggaggac ttggtttgaa gaggtttttt
     1081 agaggaatga gtatctatct tctaattata atcctaatta tgtttggcgt ccaaatgatg
     1141 gggcgaaatg tggaaacggt taagaagcta gatactactg aattaataaa gaatattaaa
     1201 gacgacaata ttaaaagtgt aagagaggtt ggaagtacca ttgagggtaa gctaaaggat
     1261 ggaacccaat ttagcactta tttaccctta tcattaagat ataattttta ccaagattat
     1321 ttacaagact tagtagacag tggtaaaatt agctatatgg gggagccaga acctggcaaa
     1381 ccttggtatt atgacctgct acctactttc cttatgttat ttgtctttgg tgttttttgg
     1441 tttatgttga tgcaacaaac tcaaggtggc ggtggaggaa gggttatgtc cttcgggaaa
     1501 agtagagccc gcctgcatag agaagatgaa ggaaaacttg ttacctttgc tgatgttgca
     1561 ggtttacatg aagaaaagga agagttacaa gaaatagtag actttttagg caatcctaga
     1621 aaatatatag atataggagc cagaattcca acaggagtac tactaatagg accacctggt
     1681 acaggtaaaa catatttaag taaggctgta gcaggggaag caggagtacc cttctttacc
     1741 atatctggtt ctgactctgt agagatgttt gtaggagtgg gagccagtag ggttagagat
     1801 ttatttgaac aagcaaagaa aaattcacca tgtatcattt ttatagatga gatagatgca
     1861 gttgggcgac atcgaggagc cggtatgggt ggtggccatg acgagagaga gcagaccttg
     1921 aatcaacttt tagtagaaat ggatggtttt ggtaccaatg aaggtattat agtaatggct
     1981 gcaaccaata gaccagatat tttagacccg gccttactaa ggccaggtag atttgacaga
     2041 caaatctatg taggtttacc agacattaga ggcagggagg aaatactaaa ggtccatact
     2101 aagaataagc ctctggcttc agatgtagac ctaaaggttg tggctagaag aactccaggc
     2161 tttacgccag cagatttaga gaacctggta aatgaggctg ctttattgtc agctagatat
     2221 aatcataagg aaataccaat gcacctaata gaggaagcat ccattaaggt gcaggctgga
     2281 ccagagaaga aaagccgggt agttagtgaa aaggaaagaa ggcttactgc ctaccatgaa
     2341 gcaggccatg ccttaactgc aagattgata cctggtatgg atccagttca tatggtaact
     2401 atcatcccta ggggtatggc tggaggattt acagcctata tacctgagga ggatgtaaac
     2461 tatagaacta agagtgaaat ggaagatagg ctggtaactt tacttggcgg tagggtaagt
     2521 gaggccctgg tcctagggga tatcagtaca ggagctcaaa at
//
LOCUS       DATGSI010000658         1831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_187145, whole genome shotgun sequence.
ACCESSION   DATGSI010000658 DATGSI010000000
VERSION     DATGSI010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187145"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VK031_10055"
     CDS             <1..256
                     /locus_tag="VK031_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HLS54308.1"
                     /translation="NNPEGLEKIGNNNYRSSNNSGEPQAGVAGAPGYGTIRQGFVEMS
                     NVDLANEFTEMIVTSRAYQANSRSITTSDEMLQELLNLKR"
     gene            321..524
                     /locus_tag="VK031_10060"
     CDS             321..524
                     /locus_tag="VK031_10060"
                     /inference="COORDINATES: protein motif:HMM:NF018043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HLS54309.1"
                     /translation="MIILTSIKGKEFCLNCELVYKVVESPDTIITLVDGKVLRVRNSM
                     EDIIEKTIAYKRQIFTNMLEEEK"
     gene            521..1324
                     /locus_tag="VK031_10065"
     CDS             521..1324
                     /locus_tag="VK031_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HLS54310.1"
                     /translation="MKRNLLPIIGSLLGMGLIIWAININGEIVNFVDISSILITVFGS
                     FCALMISFPYKTLLNIPNVMKNLLITAKDNKYELILLMSNLSRKARMEGLLALEDDMV
                     EIDNDFLTSGLQMVIDGVEPDIIKDIMELRLATLERRHRTGQEVFEKWGELAPAFGML
                     GTLIGLIVMLTRLDDPSNIGPGMATALITTFYGSLMANLVFIPIASNLSEQTDEEIFV
                     GHLIIDGLLEIQAGSNPRILEEKLTTYLSPEERELFDELRIVSEAEVYE"
     gene            1317..>1831
                     /locus_tag="VK031_10070"
     CDS             1317..>1831
                     /locus_tag="VK031_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HLS54311.1"
                     /translation="MSRRNFNRPEKNNKGAPPWMTTFSDLMSLLLTFFILLYSMSSID
                     AIKFKSISQSLQGVLSGLGYTKIVDGPSQDMEIPSQIETPLGEDKEDTLMQNILDVYN
                     LVQDYVDAQGLDANVTVDMDKRGVFVDIKEAILFEPGSAEIKEDGLKVLERLKGIFND
                     FDNEIVIEGHT"
BASE COUNT          643 a          280 c          406 g          502 t
ORIGIN      
        1 taataaccca gaaggattag agaagatagg aaataataac tacagaagct ccaataactc
       61 tggagaacca caggctggag tggcaggtgc ccccggctat ggaacaattc gtcaaggatt
      121 tgtggaaatg agtaatgtgg acttggccaa tgagtttact gaaatgattg taaccagcag
      181 ggcctaccag gccaactcaa gaagtattac cacatctgat gaaatgttac aggagctatt
      241 aaacttaaaa agatagataa atcctaggcc agtggctaaa tctcactggc ctaaggcaaa
      301 tataaagagg ggtacctgat atgataattt taacttctat aaagggtaag gaattttgct
      361 taaattgtga attagtttac aaggtagttg agtcaccaga tacaataatt acattagtcg
      421 atggtaaggt gctaagggtt agaaatagta tggaagatat aatagaaaag actattgctt
      481 acaaaaggca aatatttaca aacatgttgg aggaagagaa atgaaaagaa acttattacc
      541 tataattggt tccctactag gcatgggact gattatttgg gctattaata taaacggaga
      601 aatagtaaac tttgtagata tatcatctat attaattaca gtttttggtt cattttgtgc
      661 cttaatgatt agttttccat ataaaaccct tctaaatata cctaatgtaa tgaaaaatct
      721 cttgataaca gctaaggata acaagtatga attaatttta ttaatgagca atctatcaag
      781 aaaggctagg atggaaggac tactggcctt ggaagatgat atggtagaga ttgacaatga
      841 ctttttaact tcaggcctac aaatggtaat agatggggta gagccagaca ttataaagga
      901 tattatggag ctaaggctgg ccaccttaga aagaagacat aggacaggcc aggaagtgtt
      961 tgaaaaatgg ggagaactgg ctccagcctt tggaatgtta ggtacactaa taggtctgat
     1021 tgttatgtta accaggttag atgatcctag taatatagga ccaggcatgg ctacagccct
     1081 tataaccacc ttttatggta gtcttatggc caatttagtc tttataccta tagcatcaaa
     1141 cctaagtgaa cagactgatg aagagatttt tgtaggccat ctaatcatag atgggctgtt
     1201 agaaattcag gctggtagca acccaagaat cttagaggaa aaactaacta cctacctgtc
     1261 tccagaagaa agagaattat ttgatgagtt aagaatcgta agtgaggcag aagtatatga
     1321 gtagaagaaa ctttaacagg ccagaaaaaa ataacaaagg ggctccaccc tggatgacta
     1381 ctttttcgga tcttatgtcc ttattactaa catttttcat actcctatat tccatgtcca
     1441 gcatagatgc tattaagttc aaaagtatca gccaatcctt gcagggagtt ttatcaggcc
     1501 taggctatac taaaattgta gatgggccca gccaggatat ggagatacct tcccaaatag
     1561 aaacacctct tggggaggat aaggaagata cactgatgca aaatattttg gacgtatata
     1621 atctggtcca agactatgtg gatgcccagg gactggatgc taatgtgaca gtagatatgg
     1681 acaagcgagg agtatttgtg gacataaagg aggccatttt atttgaacca ggcagtgcag
     1741 aaataaaaga agatggccta aaggttttgg aacggttaaa ggggatcttt aacgattttg
     1801 ataatgaaat tgtaatagag ggacatacag a
//
LOCUS       DATGSI010000659         1317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_706373, whole genome shotgun sequence.
ACCESSION   DATGSI010000659 DATGSI010000000
VERSION     DATGSI010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706373"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..441
                     /gene="sepF"
                     /locus_tag="VK031_10075"
     CDS             16..441
                     /gene="sepF"
                     /locus_tag="VK031_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HLS54312.1"
                     /translation="MSSFMDKFKYFIGIEDYDDEDEVLEEEVLDAPVTTQTKRINNKV
                     LNIHTSNSMKIVVREPLTYEEAPEIVEELKSRKVVVINFEQLDINLKRQIFDFINGAL
                     YAIEGKIQKVTKDIFILAPSNVEIDGLKDELKSRGVFPW"
     gene            464..730
                     /locus_tag="VK031_10080"
     CDS             464..730
                     /locus_tag="VK031_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HLS54313.1"
                     /translation="MIIFYRSLGILVRLIEIFIIVRIFMSFVNLGRHNLFSQIVYEIT
                     EPILFPARRIIEKLGINTGMFDFSPLVAMLILRLIYDIIGRIIF"
     gene            734..>1317
                     /locus_tag="VK031_10085"
     CDS             734..>1317
                     /locus_tag="VK031_10085"
                     /inference="COORDINATES: protein motif:HMM:NF015817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HLS54314.1"
                     /translation="MYIDKEFYTAHINDRDKLVEMRRVLDKIEMVLNRHTVERTDFLD
                     PYEIRLAKSILNRFSEIGYLVDGGFKDAERKVITIYPDYYQDEINPEIRALRANVGED
                     SVSHKDYLGAILNLGIDRSKLGDILIHEDFGYILVKEEISKFILYNLERIKNMKVKLE
                     NVPLSEITVPEAKYKEISRQIASPRLDSVISAGL"
BASE COUNT          493 a          148 c          263 g          413 t
ORIGIN      
        1 attaggggtg attaaatgtc aagctttatg gataaattta aatatttcat aggtattgaa
       61 gattatgatg atgaagatga agtactagaa gaagaggttt tagatgcacc ggttactact
      121 caaactaaaa gaattaataa caaggtatta aatattcata caagcaatag catgaaaatt
      181 gtagtcagag agccactaac ttatgaagag gcacctgaaa tagtagaaga attaaaatct
      241 cgaaaagtag tagtaataaa ttttgaacag ttggatatta atctaaaaag acagatattt
      301 gattttatta atggggccct ttatgccatt gaagggaaaa tccaaaaagt taccaaggat
      361 atatttatat tagcaccaag taatgtggaa attgatggtt taaaagatga gttaaaaagc
      421 aggggtgttt tcccctggta aatatttcaa ataggagatt gttatgatta tattttacag
      481 atcgctaggt atattagtta gattaattga gatttttata atagtaagaa tatttatgtc
      541 ttttgtaaat cttggaagac ataatttatt tagtcagata gtttatgaaa taaccgagcc
      601 tatattgttt cctgccagaa gaataattga gaaacttggg attaatacag gaatgtttga
      661 tttttcgcca ttggtagcaa tgctaatttt aagacttatc tatgatatta taggaaggat
      721 tatattttaa gctatgtata tagataagga attttatact gctcatataa acgaccgaga
      781 caaattagtg gaaatgagaa gagttttaga taaaattgag atggtcttaa ataggcatac
      841 tgtggaaagg acagactttt tagaccctta cgaaataagg ttagctaagt ctatcttaaa
      901 taggttttca gaaataggct atctagtgga tggaggcttt aaggatgctg aaagaaaagt
      961 aataactatc tatccagact actatcaaga tgagataaat cctgaaatta gagccttaag
     1021 ggcaaatgta ggcgaggatt cagtaagcca taaggactat ttgggagcta tcttaaacct
     1081 gggaattgat aggtctaagc taggagatat tttaatccat gaagattttg gctatattct
     1141 agtcaaagaa gagatcagta aatttatttt atataacttg gaaagaataa agaatatgaa
     1201 agtaaagctg gaaaatgttc ctctttcaga aataacggtt ccagaggcaa aatacaagga
     1261 aatttctaga cagatagcat ctcctaggct agattcagtt atatctgctg gcctaaa
//
LOCUS       DATGSI010000660         1714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_276049, whole genome shotgun sequence.
ACCESSION   DATGSI010000660 DATGSI010000000
VERSION     DATGSI010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276049"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /gene="polC"
                     /locus_tag="VK031_10090"
     CDS             <1..1269
                     /gene="polC"
                     /locus_tag="VK031_10090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582699.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time;
                     required for leading strand synthesis; PolC exhibits 3' to
                     5' exonuclease activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HLS54315.1"
                     /translation="DVHVSSAEINRLLKGCTGIKRTSGQHPGGVMIVPDYKDILDFTP
                     IQYQADDKSSGVITTHFDYNSLSGRILKLDILGHDVPGIIKMLEDMTGVDPTKIPFDE
                     PETIEIFSSPRPLGVSEEDINCKVGTLGIPEFGTRFVRQMLVDTKPKTFSELVRISGL
                     SHGTDVWLNNAQDLVKNNIATLSEVISTREDIMLYLINAGLDKQKAFWIMENVRRGKG
                     LTEEEEVLMRNLDLPEWYIDSCNKIKYMFPKAHAAAYVMMSFRIAYFKVHYPEAFYAT
                     YFTTKVADFDAELILQGLDAVNHKINELESLGNDMTAKERNQLTVLEVVREMYLRGYR
                     FKKIDLYKSDSDKFILSDDGILPPLKGLDGVGENAAKRIVEERKISKFISVEDLVKRA
                     RLTRTVIDALTEHGCLNGLPESNQISLFNI"
     gene            1392..>1714
                     /gene="rimP"
                     /locus_tag="VK031_10095"
     CDS             1392..>1714
                     /gene="rimP"
                     /locus_tag="VK031_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HLS54316.1"
                     /translation="MGRQGGLGLVRNLCQTIADRLDYYFVDMNYVKENGNFFLRVYID
                     KKGGVNLEDCETFSELLSKELDEKDPIKGSYYLEVSSPGLDRPIKTDMDLERNLNREV
                     EISLY"
BASE COUNT          601 a          231 c          383 g          499 t
ORIGIN      
        1 gatgtacatg taagttcggc agaaataaat aggctcctaa agggatgtac agggattaaa
       61 aggacttcag gtcaacatcc aggcggagtt atgatagtgc cagactacaa ggatatttta
      121 gattttactc ctatccaata tcaagcagat gataaaagtt ctggagttat tactacacat
      181 tttgactata actccttaag cggtaggatt ttaaaattag atatactagg ccatgatgtg
      241 cccggtatta taaagatgtt ggaggatatg acgggagtcg atccaactaa aatccccttt
      301 gatgaacctg aaaccataga gatatttagt agtcctaggc ccttaggagt aagcgaagaa
      361 gatattaatt gtaaagtagg aaccttaggt ataccagagt ttggtacccg gtttgtacgg
      421 caaatgttag tggatacgaa acctaaaaca ttttctgaat tagtcaggat tagtgggctt
      481 tcacacggta ctgatgtttg gttgaataat gcacaagatt tggtaaaaaa taatatagca
      541 actctttcag aggtaatttc caccagggaa gacattatgc tatatttgat taatgcagga
      601 cttgataagc agaaggcctt ctggatcatg gagaatgtaa gaagaggtaa aggcttaaca
      661 gaggaagaag aagtacttat gaggaattta gacttacctg aatggtatat agattcctgt
      721 aataagatta aatacatgtt cccaaaagcc catgctgcgg cttatgttat gatgtccttt
      781 agaatagcct actttaaagt tcattatcct gaggcctttt acgccactta ctttaccacc
      841 aaggtggctg attttgatgc tgaattaatc ttgcaaggtt tagatgcagt aaatcacaaa
      901 ataaatgaac tggaaagcct aggtaatgat atgacggcca aggaaagaaa ccagctaaca
      961 gtattagaag tggtgcgaga aatgtatttg agaggttata gatttaaaaa gattgattta
     1021 tataaatctg atagtgacaa gttcatatta agtgacgatg gcatattacc acctttaaaa
     1081 ggtttagatg gagtagggga aaatgcggcc aaaaggattg tggaagaaag aaaaatttca
     1141 aaatttatct cggttgaaga tctggtaaaa agggctagat taaccaggac agttattgat
     1201 gctttaacag agcatggttg cttaaatggg ctacctgaaa gcaatcagat aagcctcttt
     1261 aatatttgat tttaacaaga agacatgtta taattgaact gacaataata ggactttctg
     1321 aaagagtggg gtggcccact cttgattttt tatttagtat aaatttctta ataaaaaatg
     1381 gaggtaatta tatgggtagg cagggcggtc taggtcttgt gagaaatctt tgccaaacaa
     1441 tagcagatag gctagattat tattttgtag acatgaatta tgtaaaagaa aatggaaact
     1501 tttttctaag ggtttacatt gataaaaaag gtggagtaaa cttagaagat tgtgagacct
     1561 ttagtgagtt attaagtaaa gaattagacg aaaaagatcc aataaagggt tcatactatt
     1621 tggaggtttc atctccaggg ttagataggc caataaagac agatatggat ctggagagaa
     1681 atctaaatag ggaagttgaa ataagcctat acag
//
LOCUS       DATGSI010000661         1270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_421776, whole genome shotgun sequence.
ACCESSION   DATGSI010000661 DATGSI010000000
VERSION     DATGSI010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421776"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1003)
                     /locus_tag="VK031_10100"
     CDS             complement(125..1003)
                     /locus_tag="VK031_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS54317.1"
                     /translation="MLKIRNLNKSFSTNKVLRGLNLDLEENKIYGLLGRNGVGKTTLL
                     NIVANQIKRDGGEVNFYGEELYENPRAVADICLVREKKIAAGDMKVKDIFKIAKILYK
                     NWDEAYKDYLVREFNLDLKKKYDKLSRGNQTIVGLIIGLASRAKLTIFDEPALGLDAA
                     HRDDFYNLILSDWEKNPRTIIISTHLIDEVTNLFEEVIILKEGNIFIKEEVPSLLEKA
                     YYLKGKGEDIRACVDIERIIYKESFGNTEIIGIFQNLSKEEKTSLRKKDVEISPIPLQ
                     KLFIYLTESPSIKEVS"
     gene            complement(1003..>1270)
                     /locus_tag="VK031_10105"
     CDS             complement(1003..>1270)
                     /locus_tag="VK031_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLS54318.1"
                     /translation="TNEFARIYGINPATARKGLNLLVDENILFTRRGLGMFVCKGARE
                     RILQKRQKKFLSEVLPQIVKEASRLEISLSRLIEYMEECKEGEL"
BASE COUNT          359 a          273 c          154 g          484 t
ORIGIN      
        1 ctagcacatt agatttattt aatacataat gaagtatatc tacaactagt aggacagccc
       61 aaaatttaga tagagacctt ttgctatcct catattgaaa tttaattatt ctacctgctc
      121 ccatttaact aacctccttt atagatggac tttctgtcaa gtatataaaa agtttctgta
      181 aaggtatggg gctgatttct acatcctttt ttcttaagga tgttttttcc tccttagata
      241 aattttgaaa gatgcctatt atctctgtat ttccaaagct ttctttatag attatccttt
      301 ctatatctac acaggctcta atatcttccc ctttaccctt caggtaataa gctttttcta
      361 aaagacttgg cacctcttct ttgataaata tattcccttc ctttaatatg attacttcct
      421 caaataaatt ggtcacttca tctattaggt gggtggatat tataatagtt ctaggatttt
      481 tctcccagtc acttaaaatt aaattataaa aatcatccct atgggctgca tctaaaccta
      541 gggctggctc atcaaatata gttagtttag ctcgggatgc tagacctatt attaaaccta
      601 ctatggtttg atttcctcta gaaagtttgt catatttctt tttaagatct aagttaaatt
      661 cccttactaa ataatccttg taagcctcat cccagttttt ataaaggatt tttgctatct
      721 tgaaaatatc tttcaccttc atatctccag cagctatctt cttttcccta actagacata
      781 tgtctgccac tgccctaggg ttttcatata attcttcccc gtaaaaatta acctctccac
      841 catctctttt aatttgattg gctactatat ttaatagggt ggtcttacct accccatttc
      901 tacctaatag gccatatatt ttattttcct ccagatccaa atttagacct cttaatactt
      961 tgtttgtact aaaggactta tttaaatttc taatcttaag cactatagct caccttcctt
     1021 gcactcttcc atatattcaa ttaatctact tagactaatc tccagcctgg atgcctcctt
     1081 aactatctgg ggaagaacct cagataagaa ttttttttgc cttttctgaa gtatcctttc
     1141 tctagcccct ttacaaacaa acatgcctag cccccttcta gtaaaaagaa tattttcatc
     1201 tactaaaaga tttaagccct tcctggctgt agctggatta atcccatata ttcgggcaaa
     1261 ttcatttgta
//
LOCUS       DATGSI010000662         1540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_658952, whole genome shotgun sequence.
ACCESSION   DATGSI010000662 DATGSI010000000
VERSION     DATGSI010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658952"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VK031_10110"
     CDS             <1..1443
                     /locus_tag="VK031_10110"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HLS54319.1"
                     /translation="EQFFKIREEVLNQWPTGKEVDLQDAVDYLKQVPEEKNFAIKLEK
                     AKEEGVTLAQPRAGVALIDDHIELLNYLDKEGGADFLPSTIDSYTRQNRYEECEVGIE
                     ESVKAGRSLLNGFPAVNHGVKGSRKVYESVNLPLQARHGTPDSRLLAEIIHAAGWTSN
                     EGGGISYNVPYAKAVSIEKSLRDWQYCDRLVGFYQEQGIEINREPFGPLTGTLVPPST
                     SNVTGIIEALLAAEQGVKSITVGYGQCGNLVQDVAAVRALEEQTEEYLKKFGYQDVVV
                     TTVFHQWMGGFPEDEAKAFGVISLGTTAAALAGATKVIVKTPHEAIGIPTKEANASGI
                     KATKMTINLLAGQRMPMSKELEQEIEIIKAETKCILDKVYELGKGDLAVGTVKAFEQG
                     VIDIPFAPSRYNAGQMMPARDNNGAVRYLMPGNIPFTKELIDFNRERLEERGRYENRE
                     VGFQMTIDDIFAVGKGQLIGRPEDDYGKEG"
BASE COUNT          570 a          265 c          361 g          344 t
ORIGIN      
        1 gagcaatttt ttaagataag agaagaagtt cttaaccaat ggccaactgg aaaagaggta
       61 gacctacaag atgcagtaga ctacctaaaa caggtgccag aagagaagaa ctttgcaatt
      121 aaattggaaa aagcaaaaga agagggagta accctagccc aaccgagagc tggagtagcc
      181 cttatagatg accatataga attattaaac tacttagaca aggaaggtgg agcagacttt
      241 ctaccttcaa ccatagattc ctataccagg caaaatagat acgaagaatg tgaagtaggt
      301 atagaagaat ctgtaaaagc tggtagatcc ctgcttaatg gttttccagc agtaaaccat
      361 ggggttaaag gttcaagaaa ggtatatgaa agcgtaaatt tacctctaca agcaagacac
      421 ggtacaccag attctaggct gctggcagaa ataattcacg ctgcaggctg gacttccaat
      481 gaaggtggag gaataagtta taatgtacct tatgccaagg cagtatccat agaaaagtcc
      541 ttaagagact ggcagtactg tgacagacta gtaggatttt atcaggagca aggtattgaa
      601 attaataggg agccctttgg cccactaaca ggaaccttgg taccaccgtc cacatcaaat
      661 gtgactggaa ttatagaagc cttgctggca gcagaacagg gagtaaagag cattacagta
      721 ggttatggcc aatgtggaaa cctggttcaa gacgtggcag cagttagagc cctagaagag
      781 caaacagaag aatatttaaa gaaatttggc taccaggatg tggtagtaac cacagtattc
      841 caccaatgga tgggtggctt cccagaagat gaagctaaag cctttggtgt aatatcttta
      901 ggtactacag cagcagccct ggcaggagca accaaggtaa tagtaaaaac tcctcacgaa
      961 gccataggta tcccaactaa agaggccaat gcctcaggta taaaggctac aaaaatgaca
     1021 ataaacctac tggccggcca aagaatgcca atgagtaaag agttagaaca agaaatagag
     1081 ataatcaagg cagaaaccaa gtgtatccta gacaaggttt atgaactagg aaagggagac
     1141 ttggctgtag gaactgttaa agcctttgaa caaggagtta tagatatacc atttgcacca
     1201 tccagataca atgcaggcca aatgatgcca gccagagata acaacggtgc agtaagatat
     1261 ctaatgcctg gaaatatacc atttactaaa gaactaattg actttaacag ggaaagatta
     1321 gaagaaagag gtaggtacga aaatagggaa gtaggtttcc aaatgaccat agatgacatc
     1381 tttgcagtag gtaagggtca attaatcgga agacctgaag acgactacgg taaagaagga
     1441 taatcagtct tttaagatct aactttggca aataaaaaat agggggaata gtaaatgaaa
     1501 atagttgata ttatctgttc agaaggtaaa actggctttt
//
LOCUS       DATGSI010000663         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_91582, whole genome shotgun sequence.
ACCESSION   DATGSI010000663 DATGSI010000000
VERSION     DATGSI010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91582"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VK031_10115"
     CDS             <1..518
                     /locus_tag="VK031_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HLS54320.1"
                     /translation="VVLFSNFAFLKSFNRGGYNLIPHITAGIDASAKDENFHALASSW
                     LHNQTLLELREKGNITEYQVKKIREQIKDMARLVYEHESLISDMIFEKEGIRTITKKE
                     ILHFIRNRIDFVLSNMGMRPIYGEKQGVVSEWFYNQLSSYKYSDFFSAQQIQYVRSWD
                     KSKLSFRNYVQ"
     gene            528..>2056
                     /locus_tag="VK031_10120"
     CDS             528..>2056
                     /locus_tag="VK031_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HLS54321.1"
                     /translation="MAKSYEEYSEERKVLQKAGHLPDWYTTSSFQMFDSRYKVPGEGY
                     VKERFRTIAKTLSKHMVGEEEYWEGKFFEVLWKGWLSPASPVLSNVGTDRGLPISCSG
                     GYVGDSVDEFYTSLHEQAVLSKWGFGCSGYFGDIRPRGSAISIGGKASGAVPVIEDFA
                     IMASKISQGSNRRGATASYLPIDHGDFDELVDMLEQSPDGLNIGWNVSDDFLSKLKAG
                     DAEANRRFSRALYVKLVTGKGYFFFVDRANRARPQMYKDLGLDIKASNLCTEIMLHSS
                     PSLSFSCVLSSMNLYKWDEWKDTDAVFVATVFLDCVVSEFLNKSKGIRGLEKVRKFTQ
                     LGRAIGLGVLGFSSLLQKNKIPFESLEAMYLNNGIFRKLKEDSDEASKWLAVKFGEPE
                     WCKGYGVRNTHTTALAPTKSTSLLMGGVSESVFPDPGMVFEQSSAAGGMSRITPEFYK
                     IMKERGKYNKETIDSIIANVGSVQHLDWLSDEEKLVFKTAFEVDQSVILRYASQRQKF
                     L"
BASE COUNT          662 a          326 c          482 g          586 t
ORIGIN      
        1 gggttgtgtt gtttagtaat tttgctttcc tgaagagttt taaccgaggg ggatacaacc
       61 ttattcctca tattaccgcc ggtatcgatg cttcagcaaa agacgaaaat ttccacgcat
      121 tagccagctc ttggttgcat aaccagactc tcctagagct aagagagaaa ggaaatatta
      181 cagaatacca agtcaagaaa atcagagagc agattaaaga tatggctcgg ttggtttacg
      241 aacatgaaag cttaatctca gacatgatct ttgaaaaaga gggaatcagg acaattacta
      301 aaaaagagat tcttcatttt atccggaata ggattgattt cgtactatct aatatgggta
      361 tgaggcctat ttacggagaa aaacaaggag ttgttagtga gtggttttat aatcaattgt
      421 cctcttataa atactctgac ttcttttctg cacagcaaat tcaatatgta agatcttggg
      481 ataaaagcaa actgtctttt aggaactatg tacaataaaa gggaataatg gcaaaaagtt
      541 acgaagaata tagcgaagaa agaaaagtat tacaaaaagc tgggcactta cctgattggt
      601 ataccacctc ttcttttcag atgtttgaca gcagatataa agttcctgga gaaggatatg
      661 taaaagagag atttagaaca attgcgaaaa cattgtctaa acatatggta ggggaagaag
      721 aatattggga agggaagttc ttcgaagttc tatggaaggg ttggttgtct ccggcctctc
      781 ctgtattaag taatgtagga acagacagag gacttcctat cagttgttct ggagggtatg
      841 tgggagactc tgtggatgaa ttttatacct ctctccacga acaagcagta ctatctaaat
      901 gggggtttgg atgctctgga tatttcgggg atattcgccc ccgaggaagc gctattagta
      961 ttggagggaa ggccagtgga gcagttcctg taatagaaga ttttgctatt atggccagta
     1021 agatcagcca agggtctaat agaagagggg caactgcatc ctaccttcca atagatcatg
     1081 gggatttcga tgaactagta gatatgttag agcaatcccc tgatggccta aatattggtt
     1141 ggaacgtaag cgacgatttc ctatctaaat tgaaagcagg agatgccgag gctaatagga
     1201 gattctctag ggcactgtat gttaaattag taacaggaaa agggtatttc tttttcgtag
     1261 acagagccaa tagagccaga cctcagatgt ataaagactt aggactagat attaaggcta
     1321 gcaatctgtg tacggaaatt atgctccata gttctccttc tttgtctttc tcttgtgtac
     1381 tttccagtat gaacctgtat aaatgggatg aatggaaaga cacagatgca gtgtttgttg
     1441 ccacagtttt cctagattgc gtagtatcag agttccttaa taaaagtaaa ggtattaggg
     1501 gcctagaaaa agtacggaag tttactcaac tagggagagc aatagggcta ggggtgttag
     1561 ggtttagtag ccttcttcag aagaataaaa ttccttttga gtctttagag gctatgtacc
     1621 taaataacgg tatatttaga aaattaaagg aagattcaga cgaggcctct aagtggttgg
     1681 cggttaagtt tggagagcca gagtggtgta aaggttatgg agttcgtaac acccatacaa
     1741 cagctttagc tcctactaaa tctaccagcc tattaatggg aggagtttct gaatctgtat
     1801 tcccagaccc aggtatggta ttcgaacaat cctccgcagc agggggtatg tccaggatta
     1861 cacctgagtt ctataagatt atgaaggaac gagggaaata taataaagaa acaatagaca
     1921 gtattatagc taatgtagga agtgtgcaac atttagattg gctctctgac gaagaaaaat
     1981 tagtatttaa gactgctttt gaagtagacc aaagtgtaat tcttaggtat gcttctcaaa
     2041 gacaaaagtt cttaaa
//
LOCUS       DATGSI010000664         3406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_659068, whole genome shotgun sequence.
ACCESSION   DATGSI010000664 DATGSI010000000
VERSION     DATGSI010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659068"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="VK031_10125"
     CDS             <1..1303
                     /locus_tag="VK031_10125"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HLS54322.1"
                     /translation="DEFLSKEKELEDKDKAIQLKYEEISYTIKLQDIGYGYDFDAAID
                     EAFAMGRDGGPIKRYKEVKNIEKNGIFIPLESHYDKGKIEKISQEIAEEMYVEEIDAT
                     FNFNGGKFKVTEDSPGRKVDEKKLSQLIEENIGKWEDIEIPVDIIEPKYTKEYYSRIN
                     GVIGEFSTDYSTSIPGRKHNIRLSAGKFNGKLLHPGDSISYNKTLGPITKAAGYKEAS
                     VIENGEFVTGVGGGICQTSTTLYNALLQAGLTIVQRSPHSIPIGYVPKGTDAAVAAGW
                     KDLVFKNDYDFPVYLTSKNVGDRVYFQIYGDTKVKDYQVRIESKIVETIKHKVKENYL
                     KNARPGYRKVVQTGRDGYKVKTYKYKIKNGKVIESKQISYDYYRERDAIVEVGPKKEE
                     NKPKEKEKVEANEKEDKGQDEEGKNEKEETREKKDESNEED"
     regulatory      1399..1576
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1683..2672
                     /locus_tag="VK031_10130"
     CDS             1683..2672
                     /locus_tag="VK031_10130"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HLS54323.1"
                     /translation="MKRRNLRFLSLILVILLSLSVLAGCTTGTDQDTKEPEDPIVEED
                     GNEVEESAFPLEIEDKFGNQAEFEQAPEKIVSLAPSHTEILFALGLGDKVVGIDSNSN
                     YPEEATEKEVVGDYYGTDLEKIIEMEADLVLAYGAGNEEDNARLKEAGIPVLGYMPET
                     IEEVFETIIEIGKITDSLDKANTLVDEMKAKRDEIVDKVKDADKVRVFYEIWHEPLQA
                     AGPGSFMDELINLAGGENIAHDSQAAYAEYDLEQLIERDPEVYLTAEMTEPIEGEEKI
                     TAETIAARPGYEDITAIKDGRIYLLDGDIVSRPGPRIIDALELVAKALHEDLF"
     gene            2687..>3406
                     /locus_tag="VK031_10135"
     CDS             2687..>3406
                     /locus_tag="VK031_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HLS54324.1"
                     /translation="MKISRDEKKYPIILIALTITLIFAIALFSTIGTANVGVMDVFKI
                     VVEKIPFLNRFVDMSKITDSHRTIIWNIRLPRVILGVLVGASLSMAGAAFQGMFKNPM
                     ADPYIIGISSGAALGATIAIILKLDYRIYNISTISVFAFLGALLTVFLVYNIARIGGS
                     LPITNLLLAGVAIGQFFTAIMSFLMIIYDNDMNKIIYWTLGSLSGKGWEPVNRIFLLV
                     VTVMILINFFARDLNIMLTGDE"
BASE COUNT         1249 a          466 c          766 g          925 t
ORIGIN      
        1 cgatgaattt ttgtctaagg aaaaggaact ggaagataaa gataaggcta tacagcttaa
       61 atatgaggaa ataagttata ctataaaact tcaagatatt ggatatggtt atgattttga
      121 tgcagctata gatgaggcct ttgccatggg cagggatggt ggccctatta aaagatacaa
      181 agaggtaaaa aacatagaaa aaaacggtat ttttattccc ttggaatctc attatgataa
      241 ggggaaaata gaaaaaataa gccaggaaat tgctgaggaa atgtatgttg aagaaataga
      301 tgctaccttc aactttaatg gaggaaaatt taaggttaca gaggattcac caggtagaaa
      361 agtagatgaa aagaagttaa gccaactaat tgaagaaaat ataggtaaat gggaagatat
      421 tgaaatacct gttgatataa tagagccaaa atataccaag gaatattaca gcaggataaa
      481 tggtgtaata ggtgagtttt ccacagacta ttctaccagt atacctggta gaaagcataa
      541 tattagattg tcagcaggaa agtttaatgg gaaactattg cacccaggag atagcatatc
      601 ctataataaa accctaggac ctattaccaa ggctgcaggg tataaggaag catcagtaat
      661 agaaaatgga gaatttgtaa ctggagtagg gggaggaatt tgccaaacat ctactaccct
      721 ttataatgcc cttttgcagg caggactgac cattgtccaa agaagtcccc attccatacc
      781 tataggttat gtacctaaag gtacagatgc agcagtagca gcaggttgga aggatttagt
      841 ctttaaaaat gattatgatt ttccagtata tttaacttca aaaaatgtgg gagacagggt
      901 atattttcag atttatgggg ataccaaggt taaggactac caagtaagga ttgaatcaaa
      961 aattgtagag actataaagc ataaggtgaa ggagaactat cttaaaaatg ctaggcctgg
     1021 atacaggaag gtagttcaaa caggaagaga cggatataag gttaaaactt ataaatataa
     1081 gattaaaaat ggtaaagtta tagaaagcaa acaaatcagt tatgattatt atagggaaag
     1141 agatgccata gttgaagtag gccctaaaaa agaggaaaat aagcctaaag agaaagaaaa
     1201 agtcgaagca aatgaaaaag aagataaggg ccaagacgaa gagggaaaga atgagaagga
     1261 ggagacaagg gagaagaaag atgaaagcaa tgaggaagat taaaaggtaa gagaagggta
     1321 aaatatgatt ttgttggcct tgctggacct taaaaggatt agcttgaagt aagttaatta
     1381 atttgatatc ataattatga gaaaattaaa taattaggcg tcccattagg gacttaaggg
     1441 aaagtggtgc aaatccacta cagcccccgc tactgtaatt gggaacgaaa ccaacactgc
     1501 cactgggaaa ctgggaaggc gttggaagta ggatgatcca taagtcagga tacctgccta
     1561 actatatatt gcctcggagg gagggatgag atgaagatat aaaacttatc ctagaaaata
     1621 ggcataagtt ttttcttttg tttaacaaaa ctcaaatttt ataatttcag gaggagaact
     1681 ttatgaaaag aagaaattta agatttttat ctttgattct tgttatacta ctgtcactat
     1741 ctgtattagc cggatgtaca actggaactg accaagatac aaaagagcca gaggatccta
     1801 tagtagaaga agatggtaat gaagtagagg aatcggcctt tcctctagaa atagaggata
     1861 aatttggtaa ccaggctgag tttgagcagg caccagaaaa aattgtatct ttagcaccaa
     1921 gccatacgga gattcttttt gccctaggcc taggagataa ggtagttggt attgattcca
     1981 acagtaatta tccagaggaa gccactgaaa aggaagttgt aggcgactat tatggaactg
     2041 atttagaaaa gataattgag atggaagctg atcttgtatt agcctatggt gcaggcaatg
     2101 aagaggacaa tgctagacta aaggaagccg gaataccagt tttaggatat atgcctgaga
     2161 caatagaaga agtttttgaa actataattg aaataggaaa aattacagac agcttagata
     2221 aggccaatac cttagttgat gagatgaagg ccaagagaga tgaaatagtt gataaggtaa
     2281 aggatgcaga taaggtaagg gtattttatg aaatatggca tgaaccattg caggctgcag
     2341 gcccaggttc ttttatggat gagctgataa atctggccgg cggagaaaat atagcccatg
     2401 atagccaggc tgcttatgca gagtatgatt tagagcagct aatagaaagg gatccagagg
     2461 tttatctaac agctgaaatg acagagccaa tagaaggcga agaaaaaata acagctgaaa
     2521 ccatagcagc cagaccaggt tatgaagata taacagccat taaggatggt agaatctatc
     2581 tattagatgg agacatagta tctagaccag gaccaaggat tatagatgca ttagaactgg
     2641 tagccaaagc tttacatgaa gatctatttt agaaaagttg gtttaaatga aaattagcag
     2701 agatgaaaag aaatatccta ttattttaat agccttaact ataaccttaa tatttgcaat
     2761 agctctattt tccaccattg gtacagccaa tgtgggagtt atggatgtat ttaaaatagt
     2821 tgtggaaaag attccatttc taaatagatt tgtagatatg tctaagatta cagattcaca
     2881 taggaccata atttggaaca ttagactgcc tagggttatt ttaggagtgt tagtaggtgc
     2941 cagcctttcc atggctggtg ccgcctttca aggcatgttt aaaaacccta tggctgatcc
     3001 ttatattata ggtatttcat ctggggctgc tttaggtgct accattgcaa taatattgaa
     3061 attagattat agaatataca atatatccac catctcagta tttgcctttt taggagccct
     3121 acttacagtt tttttagttt ataatattgc cagaattgga ggcagtctac ccataaccaa
     3181 tctcctttta gcaggagttg ccataggaca attttttact gcaattatgt catttttaat
     3241 gatcatttac gacaatgata tgaacaagat aatttattgg accttgggca gcctatcagg
     3301 taaaggctgg gaaccggtaa atagaatttt tctattggtg gttacagtta tgatactgat
     3361 taattttttt gccagagatt taaatattat gttaacagga gatgag
//
LOCUS       DATGSI010000665         1977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_659104, whole genome shotgun sequence.
ACCESSION   DATGSI010000665 DATGSI010000000
VERSION     DATGSI010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659104"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VK031_10140"
     CDS             <1..584
                     /locus_tag="VK031_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HLS54325.1"
                     /translation="DIDSQYADLYEEIADIAIHIIENGDVIMLTNGIINYHIAKKLNK
                     KKNITVLTNDLMISIEAASSLSNKAVLLGGDIDYEAKATFGALTAVNIQKFFVNKLFI
                     EVDGIDIDTGLSVASMDKANLINSILPNSKEKIILCLGSNFNKTAFYNISDINIAGKI
                     VTNPNIDDYYKKFISEHGIQLYTSINAFEGGNT"
     gene            584..>1977
                     /locus_tag="VK031_10145"
     CDS             584..>1977
                     /locus_tag="VK031_10145"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLS54326.1"
                     /translation="MASPILSLININKKISDTFQLSNISADFYPGEVCAVIGENGSGK
                     SSLMKVISGILTPDSGEILFNNKPVAFKSIYDAKKQGIYYVPQESNLFDNLTVAENIF
                     IDSVSHRYNLYEAVNTNEVLHNAGQLLEELNINMEVEEYVKNLNLSQKQILSFVKAYV
                     INARFTIFDEPASSLTDIENATLFKIIDLLKANGSSIILISHKLERIMEIGDKVIILK
                     DGSIIEKGSIKDYTNESIIKIMSKSTGTSKFPKLYFDNKDEILSLSNISYKRFLKDIS
                     FSMKKQEILGVIGNSGSGKDKLIRILFGLLKPDSGEIKLEGSNIDLTHPKDAMDKGFG
                     LVPEDKIRNSIFHQLDLVNNLTMSSLGRFTRNIVLDDYIMTNVAENYISKLRISPGNP
                     DDRAIHYSGGNQQKVAFAKSIMHLAKIYILDEPTRGIDIVSKNDIYNIMNNLLINGSS
                     IILFSSDFDEILGMS"
BASE COUNT          719 a          304 c          334 g          620 t
ORIGIN      
        1 ttgacataga ctcccaatat gcagatttat atgaggaaat tgcagatata gctatacata
       61 taatagaaaa tggagatgta ataatgctaa ccaatggcat tattaattat catattgcaa
      121 aaaagcttaa caaaaagaaa aatattacag tacttaccaa tgacttaatg atttccatag
      181 aggctgcctc aagtctgtct aataaggcag tacttttagg tggagatatt gattatgagg
      241 ctaaggcgac ctttggagcc ttaaccgctg taaatataca aaaattcttt gtaaataaat
      301 tatttataga ggtagatggt attgatattg atacaggatt aagtgtagca agtatggata
      361 aggccaactt aattaatagc atcctgccta attccaagga gaagataata ctatgtctgg
      421 gctccaattt caataagact gcattttata atataagtga tattaacata gctggtaaaa
      481 tcgttaccaa tccaaatatt gacgattact acaaaaaatt catttcagaa catggaatcc
      541 agctatatac atccataaat gcatttgaag ggggtaatac ataatggctt cccctattct
      601 ttcactgatt aatattaata aaaagatttc ggataccttc caactttcta atattagtgc
      661 agacttttat cctggagaag tatgtgcagt aattggtgaa aatggttccg gtaaatccag
      721 tttaatgaag gtcatcagtg ggatattaac cccagatagt ggagaaattc tattcaataa
      781 taaacctgtt gcatttaaat ctatttacga tgccaaaaaa cagggtattt actatgtacc
      841 tcaagagtcc aatctatttg ataatttaac tgttgctgaa aatatattta ttgactcagt
      901 ttcacataga tataatctat atgaagcggt aaatacaaat gaagttctcc acaatgccgg
      961 acaactcctt gaagagctta atattaatat ggaagttgaa gaatatgtaa aaaatcttaa
     1021 tttatcacaa aaacaaatcc tttcctttgt aaaggcctat gtaataaacg caagatttac
     1081 catatttgat gaaccagctt cttccttaac agatatagaa aatgctacct tgtttaaaat
     1141 aatagacctt ttaaaggcta atggtagctc aattatttta atttctcaca aattggagag
     1201 gattatggaa ataggggaca aggttatcat tctaaaggat ggaagtatta ttgaaaaggg
     1261 aagtattaag gattatacta atgaatccat aattaaaatt atgtctaagt ccactggcac
     1321 tagcaaattt cccaagcttt atttcgacaa taaggatgaa atactgtccc ttagcaacat
     1381 cagttacaaa agatttttaa aggacatatc cttctccatg aaaaaacagg aaatcttggg
     1441 ggtaattggt aattctggtt caggaaagga taaattaatt agaattctct tcggtctatt
     1501 aaaacctgac tctggagaaa taaaattaga aggttccaat atagatttaa cccatcccaa
     1561 ggatgccatg gataaaggtt ttggtctagt acctgaagat aagatacgaa actccatatt
     1621 ccatcagctg gacctagtta ataatttaac catgtcttca ttgggccgct tcacaagaaa
     1681 tatagtattg gatgattata taatgaccaa tgtggccgaa aattatattt ctaagcttcg
     1741 catatctcca ggaaatccag atgatagggc tatacactat agtggaggta atcaacaaaa
     1801 agtagcattt gctaaaagta ttatgcactt ggccaagata tatattctgg acgagcctac
     1861 tagaggaata gatattgtat ccaagaatga tatatataat ataatgaata atcttttaat
     1921 caatggttca tctattatcc tcttttcctc agatttcgat gaaatacttg gtatgtc
//
LOCUS       DATGSI010000666         1332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_565992, whole genome shotgun sequence.
ACCESSION   DATGSI010000666 DATGSI010000000
VERSION     DATGSI010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565992"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /gene="prs"
                     /locus_tag="VK031_10150"
     CDS             <1..333
                     /gene="prs"
                     /locus_tag="VK031_10150"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HLS54327.1"
                     /translation="VMNVIGDIEGKDAVMVDDIIDTGGTIVNAATILKEMGAKRVFGC
                     ATHGVLSGPAVDRICDSVIEKFIITDTIPLKKKCEDDKIEVVSVAPLFAEAIQRINEN
                     RSVSVIFD"
     gene            349..912
                     /gene="pth"
                     /locus_tag="VK031_10155"
     CDS             349..912
                     /gene="pth"
                     /locus_tag="VK031_10155"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HLS54328.1"
                     /translation="MYVIVGLGNPGEKYANTRHNVGFKVIDLLAKRNNIKVNKIKFQS
                     LYGEGNIAGKKVALIKPITYMNNSGVAVREIKDFYKLEAENILVIVDDVDIKFASIRI
                     RKKGSAGSHNGLKSIIQHIRTENFPRIKLGIGQKSHNQDLADFVLGEFSKEEESIIED
                     CILLTARAVETMLEKGIHQAMNEYNKR"
     gene            966..>1332
                     /locus_tag="VK031_10160"
     CDS             966..>1332
                     /locus_tag="VK031_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HLS54329.1"
                     /translation="MTDFLLDTLKQLDSYKGLIDNIKKEISPLATYGIIDENLGHFIY
                     GLNTHLKKQIILITDNEAKARHLYEDIKSLGKTNVDLLPSREIIFYDIDASSHDNRKQ
                     RLSVISKLINKEEGIIITTV"
BASE COUNT          512 a          159 c          291 g          370 t
ORIGIN      
        1 gttatgaatg ttattggtga tatagaagga aaagatgctg ttatggtaga cgatattata
       61 gacacaggtg gaactattgt aaatgctgca actattttaa aagaaatggg ggccaagaga
      121 gtatttggct gtgcaaccca tggtgtatta tcgggacctg ctgtagatag gatatgtgat
      181 tcagttatag agaaatttat aattacagat actatacctt taaagaagaa atgcgaagat
      241 gataagatag aagtagtaag tgtggcacct ttatttgctg aagccataca gaggatcaac
      301 gaaaacagat ctgtaagtgt aatatttgac taggcagggg atgattattt gtacgtaatc
      361 gtaggcttag gtaatccggg agaaaagtat gctaatacta ggcataatgt aggctttaag
      421 gtaatagatt tattagcaaa gaggaataat attaaagtaa ataaaataaa gtttcaatcc
      481 ctttatggag agggaaatat agcaggtaaa aaagttgcac taataaaacc tataacatat
      541 atgaacaaca gtggagtagc agtaagggag attaaagatt tttataagct ggaagcagaa
      601 aatattttgg ttatagtaga tgatgtggat attaaatttg ccagcataag aattagaaaa
      661 aagggaagtg caggttccca caatggctta aaatcaataa tccaacatat ccgaactgaa
      721 aactttccta gaattaagct aggaataggg caaaaaagtc ataatcagga cttggctgac
      781 tttgttctag gtgaattttc taaagaagaa gaaagtatta tagaggattg catactattg
      841 acagctaggg cagtagagac catgctggaa aagggaatcc atcaggcaat gaatgaatat
      901 aataaaagat aaaatagggc ccaggatcca acctgggctt aaggtgtttc caaggggtaa
      961 gatatatgac tgatttttta cttgatacat taaaacaatt ggattcttat aaggggctta
     1021 tagataatat taagaaagaa ataagcccac tggcgactta tggaattata gatgaaaatt
     1081 taggccattt tatatatgga ctgaatactc atcttaagaa gcagataatt ctaataactg
     1141 ataatgaagc gaaggctagg catctgtatg aggatattaa aagtctaggc aaaaccaatg
     1201 tggatttgtt accaagtaga gaaattattt tttatgacat agatgcatca agccacgata
     1261 accgaaaaca aagattaagc gttatatcca aactaataaa taaagaggaa ggtataatta
     1321 taactactgt tg
//
LOCUS       DATGSI010000667         1608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_374305, whole genome shotgun sequence.
ACCESSION   DATGSI010000667 DATGSI010000000
VERSION     DATGSI010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374305"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VK031_10165"
     CDS             <1..613
                     /locus_tag="VK031_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54330.1"
                     /translation="NVDKVYDWIVEESTGTATIPIGEFPITIPVDATNVKVKCMLTDE
                     LGNPIPINSELDVMETSARQDHRLKVGGMSVTLQRVSFTKTLYAVLEVRGVDPATGNQ
                     FLVTSDAIPFNFFETALLCAPAGTNLLVKISNFDCLTFVTRNEAGEITDFGLNISICQ
                     SIQTVAPVTVEMEANLCNPRREIIEHCATPGLPGDCSAIFPED"
     gene            complement(1335..>1608)
                     /locus_tag="VK031_10170"
     CDS             complement(1335..>1608)
                     /locus_tag="VK031_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54331.1"
                     /translation="GLILTISRKYNIEYGQTLDGYKVIRKYIKDNKQKIFKDQNQIDS
                     YFDLVHTRNSAVHGSVGRGTQNYLEQQEHFEELFKNGINIYEYITR"
BASE COUNT          502 a          311 c          237 g          558 t
ORIGIN      
        1 aaatgtagat aaggtatacg attggattgt agaagaatcc actggaacag ccaccattcc
       61 aataggagaa tttcctatta caatccctgt agatgctact aatgtaaagg ttaaatgtat
      121 gctgactgat gaacttggta atccaattcc aataaactca gagttagatg ttatggagac
      181 ttctgctagg caagatcata gattgaaagt aggcggaatg tctgtcactc ttcaaagagt
      241 ttcctttacc aagactttgt atgctgtcct agaagttagg ggagttgacc cagccactgg
      301 caaccaattt ttagttactt ctgatgcaat accctttaat ttctttgaaa cagccctact
      361 atgtgcacct gctggcacta acttactggt aaaaatttca aactttgact gtttaacatt
      421 tgtaacaaga aatgaagctg gagaaattac tgactttgga ttaaatatct ctatatgtca
      481 aagcatccag acggtcgccc ctgttacagt ggaaatggaa gctaatctat gtaatcctag
      541 aagagaaata atcgagcact gcgctacacc aggccttcca ggtgattgtt ctgctatttt
      601 cccagaagat taaagatcag ttttaatata aataaattca accaataata aaaacaaggg
      661 gacttaagtc cccttgtttt tattatcacc tattctatta tttatgtctg ccaatctctt
      721 aatgcctaaa acatctatgt ttaccctatt ttataccaca gttacccctt cttgaaaagt
      781 catccacctt acacactatt tcacagctta caaattatat tatgtataat ctctcgagta
      841 aatctggtag ttccactaat agttttccag cttcgtcaag cttgtaacca tagtttccct
      901 tcttttaacc tgtagtacca aataaagtaa taatcttttc caacacacca ttcacaccct
      961 aatataatca tgtctattac attctatatt tagaataaga ttccttattt atatataagt
     1021 tcccatacca cttaaaagtg atatgggaat ttctttaaaa tccttatagg tagtctaata
     1081 actttcttca ccatatgatg attttaataa ctctataggg tttcaaatcc ttataggtag
     1141 actaataacc cgttacatac attgatatta ctacattcct atttttctgt caaatggtgt
     1201 tttccaagat tttaggatgt caaaattgtc ctgtattagt ccttacacta gcaaacaagc
     1261 tatacatagc tcttttatat aaaaatgtcg acccccaggg gtttttgcac tactgggggt
     1321 cgacgacaaa tattttatct agtaatatat tcatatatgt tgataccatt tttaaacaat
     1381 tcttcaaaat gttcttgttg ttctagataa ttttgtgtcc ctcttccaac ggaaccatgg
     1441 acagcggaat tcctagtatg tactaaatca aagtaggaat caatctgatt ttgatcctta
     1501 aaaattttct gcttgttatc cttgatgtat tttcttatga ccttgtatcc atcaagagtc
     1561 tgcccatatt ctatattata ttttctgcta attgttagaa taagtcct
//
LOCUS       DATGSI010000668         1458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_706796, whole genome shotgun sequence.
ACCESSION   DATGSI010000668 DATGSI010000000
VERSION     DATGSI010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706796"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VK031_10175"
     CDS             complement(<1..1003)
                     /locus_tag="VK031_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLS54332.1"
                     /translation="MYDDKDYLDEENQMDLEDSEEIEEDFTEDVHDEKDLINDKEPKR
                     KKSFRGSLISYLALAIVASLLGGIASAYIGPSLYGKILPNPKSNEYVSQPVNIATSDD
                     INTVSAVVKKAMGSVVGITTLETRQYPFQQRDVEGLGSGVIVDRNGYILTNSHVVANG
                     KAKEITVLFDNGDNVSGKVLWNDPILDLAIVKVDITNLPAATLGDSDELEVGEIAIAI
                     GNPLGLEFQRTVTSGIISGLNRSIKIDQNTVIDDLIQTDASINPGNSGGPLLNKNGEV
                     IGINTAKIQSGEGLGFSIPINTAKSIIEEVISKGSYETVELGITGLDINEYQARLGID
                     "
     gene            1203..>1458
                     /locus_tag="VK031_10180"
     CDS             1203..>1458
                     /locus_tag="VK031_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLS54333.1"
                     /translation="MSYKIYLVEDDENLNLVLTSYLVSEGWQVTSFLTGQEAKQHIDS
                     PPDLWILDIMLPDIDGYQLIRMIKEKSPQVPVIFISARDAD"
BASE COUNT          409 a          289 c          236 g          524 t
ORIGIN      
        1 aatctattcc taatctggcc tgatactcat ttatatctag accagttatt cctaattcaa
       61 ctgtttcata gcttcccttg ctaattactt cttctattat acttttagct gtatttattg
      121 gaatggaaaa acctagtccc tctcctgatt ggattttggc tgtatttata cctatcactt
      181 ccccattttt atttaataag gggccgccac tattacctgg atttattgat gcatctgttt
      241 gtataaggtc atcaattact gtattttggt ctatctttat ggatctattt aggcctgaga
      301 ttatgcctga agtaactgtt ctttgaaatt ctaatcctaa aggattacct attgctatgg
      361 ctatctctcc cacttccaat tcatcagaat ctcctaaagt ggcggcaggt aggttagtta
      421 tatcaacctt cactatagcc aagtctagga taggatcatt ccacaaaacc ttcccagata
      481 cattatctcc attgtcaaag agtacagtaa tctccttggc cttgccattg gctaccacat
      541 gggagttggt taaaatatat ccatttctat ctactataac accagaacct agcccttcca
      601 catccctttg ctggaatggg tattgtctag tttccaaggt ggtaatacct actactgatc
      661 ccatagcctt ttttaccaca gctgaaactg tgtttatatc gtcgctggtt gcaatattta
      721 ccggctggga tacatattca ttgcttttag gattgggaag tatttttcca taaagactgg
      781 gccctatata ggcagatgct ataccaccca gtaaagaagc cactatggcc aatgccaaat
      841 aagatattag actgcctcta aagcttttct tcctttttgg ctccttgtca ttaattaaat
      901 ccttttcatc atgaacatct tctgtaaaat cttcttctat ctcttcacta tcctcaagat
      961 ccatctgatt ttcttcatct agataatctt tatcatcata catccagatt cctccctgtg
     1021 acttaggtaa aacttccagt taatcgttac ttatcctact ttaatttatt agcaatttaa
     1081 tattatttaa taaatgtaaa aagaatatgg gtaaattatg tttttgttgt gtgatttagc
     1141 tttagtaggt ttaatttggt ataataaatt tacaagttca ataggagtgt aaaggtgatt
     1201 atatgtccta taaaatatat ctagtggaag atgatgaaaa tttaaacctg gtacttactt
     1261 cttacctggt cagtgaaggc tggcaggtga catctttttt gactggtcag gaagcaaaac
     1321 aacatataga ctctccccct gacttatgga ttctggatat tatgttgcct gatatagacg
     1381 gttatcagtt aattagaatg attaaggaaa aaagtccgca ggtgccagtc atatttattt
     1441 cagccaggga tgcagata
//
LOCUS       DATGSI010000669         1392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_276689, whole genome shotgun sequence.
ACCESSION   DATGSI010000669 DATGSI010000000
VERSION     DATGSI010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276689"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1220)
                     /locus_tag="VK031_10185"
     CDS             complement(84..1220)
                     /locus_tag="VK031_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54334.1"
                     /translation="MEYIDQYFKRKTKEISFLELKEEAQLVIKNKELKQVPLPILTDK
                     LIDDIKSNSLDEGVELSRFVDGFIYTLGADPDFPYNKEYKKILLSLDSKVYGFVFTKA
                     MEDYKSNRLDDSAIRLRGLLYIEPNNVDFLFNYGIVMEEIGKRLIERDQLEEGEKFIK
                     ISTDQFETILDVDEEYSFAYYKLGFHYKYFERYLKADLIWKKFLTLSKNDLLKQEIRE
                     ELEKIKDDSSFETGLTYLNYNDYERALDSFLTLIPKHKESWNVNYLVGRCYSGLGQFN
                     MAADYLSKAIELNDQEADLYNELGILYFNIGNINKALETFSQGIEKNIEDYKLFFNRG
                     LAYVELGQPKKGLKDIEKAYSLQPNDENIKRQKEHLENILLDEK"
BASE COUNT          414 a          264 c          177 g          537 t
ORIGIN      
        1 atttatttat attttcatcc ttttcggctt gaaatagacc tggataagtc aattttatcc
       61 tgctaacctt tcaattaata aaactatttc tcatctaaaa gaatattctc tagatgttct
      121 ttctgtcttt taatgttttc atcatttggt tgtaggctat aggctttttc aatatccttt
      181 aaacctttct taggttgtcc tagctccaca taagccagac ccctattaaa aaataattta
      241 tagtcctcta tatttttttc tattccttgg ctaaaagtct caagagcctt atttatatta
      301 cctatattaa agtatagaat tcctagttca ttatataggt ctgcctcctg atcatttaat
      361 tctatggcct tagataaata gtctgcagcc atattgaatt ggcccagccc actgtagcat
      421 cttccgacta gataatttac attccaactt tccttatgtt taggtattaa tgttaagaag
      481 gaatctaagg ccctttcgta atcattatag tttaaatagg taaggccagt ttcaaagctt
      541 gaatcatcct ttatcttttc taattcttcc ctaatttcct gtttaagcag atcatttttg
      601 cttaaggtca aaaacttttt ccatattaga tctgccttta gatatctttc aaagtattta
      661 taatgaaagc ctaatttata ataggcaaag gaatactcct catccacatc taagatggtt
      721 tcaaattggt cagttgaaat ttttataaat ttttcccctt cctcaagttg atccctttct
      781 attagcctct tccctatctc ttccattact atcccgtagt taaatagaaa atctacatta
      841 ttaggctcta tatataaaag tcccctaagt cttatggcgc tgtcatctaa cctattagat
      901 ttatagtcct ccatggcctt tgtaaaaaca aatccatata ctttagagtc taaggatagt
      961 aaaattttct tatattcttt attataggga aagtctggat ctgcccctag ggtgtagata
     1021 aatccatcaa caaacctact taactctacc ccttcatcca ggctattaga cttaatatca
     1081 tctattaact tatctgttaa aataggtaaa ggcacttgtt ttaactcttt gtttttaatt
     1141 accaattgag cctcttcctt taattctaag aaggaaattt ccttggtttt tcttttaaaa
     1201 tattgatcta tatattccat attatcacct aagtttaaaa tttattcata aagcgtctct
     1261 ttctcctggt actggtactt aaaatcttaa ataaatgccc tctgtagacc atcataaagg
     1321 agaaaattaa ttggcctagt aaataccttg taatatatac tggctttagg atagtctgaa
     1381 aggttaagtg gg
//
LOCUS       DATGSI010000670         1696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_325431, whole genome shotgun sequence.
ACCESSION   DATGSI010000670 DATGSI010000000
VERSION     DATGSI010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325431"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1661
                     /locus_tag="VK031_10190"
     CDS             <1..1661
                     /locus_tag="VK031_10190"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HLS54335.1"
                     /translation="IVKDNVYIIGHKNPDSDSICSAIAYADFKNSSSDVNAIPVRLGE
                     INRETKFILDYFDVEVPQLMETVRLSVEDLNFDNVSPVSPDISLRMALNIMTNNDLNS
                     LPVIKGNEQLVGIVTVSDIMQSYIDVWDNSILGKAKTSIDNILDTLSAKALLIPEEMR
                     KMAGKILVLAMDATSSKEYLEEHDIVICGDRGDVLEMAIKSKISLLIVTGGSKIKDEL
                     IELADKKGVAIISTPHDTFTTARLISQSIPIEQVMTKDNLVVFRLDDLVDEVKVQMSQ
                     TRYRSYPVVDHENKVIGLISRYHLISSMKKKVILVDHNERSQSIDGLEEAEILEIIDH
                     HRVADVFTGSPIYFRNEPVGSTCTIIASIMFENGRRPSKKIAGILSAGIISDTLLFKS
                     PTSTKTDRIMLERLARIADLDVEEFAAEMFKAGTSLTGKTPDELLNQDFKSFNINEKK
                     VGIAQVYTMDLESLDGMKDSLIQLMQERAREYNYSIFMLMLTDIFREASEMIAVGKNK
                     ELVGQAFGKELINNSLYAPGVLSRKKQVVPPITNTLTNIQEMLR"
BASE COUNT          612 a          245 c          351 g          488 t
ORIGIN      
        1 atatagtgaa ggacaatgta tatataatag gccataaaaa tccagattca gattctatat
       61 gttctgcaat agcctatgct gattttaaaa atagtagtag tgatgtaaat gccatacctg
      121 ttaggttagg agaaattaat agggaaacta aatttatttt agattatttt gatgtagagg
      181 tccctcaatt aatggagact gtaagactaa gtgttgagga tttaaatttt gataatgttt
      241 caccagtaag tcctgatatt tccttaagaa tggccttaaa cataatgacc aataacgatt
      301 taaacagcct acctgtaatc aaaggaaatg aacaactggt aggtatagtt actgtttctg
      361 atataatgca gtcttatata gatgtttggg ataattctat cctaggcaag gcaaagacct
      421 caatagacaa tatactggat accttgtctg caaaggcctt attgattcca gaagaaatga
      481 gaaaaatggc agggaaaatt ttagttctgg ccatggatgc caccagttcc aaggaatatc
      541 ttgaggaaca tgatattgtt atatgtggtg acaggggaga cgttctggaa atggccataa
      601 aaagtaagat ttccttgttg attgttacag gcggtagcaa aataaaggat gagctaatag
      661 aattggcaga taaaaaaggt gtggccatta tatctacacc ccatgatacc tttactactg
      721 ccagattaat atcccagagt attcctatag aacaggtaat gactaaagat aatttggtgg
      781 tatttaggct agatgatcta gtggatgagg taaaggttca aatgtctcag accagatata
      841 gatcatatcc ggtggttgac catgagaaca aggtaatagg cctaatatct agataccatt
      901 taatatccag tatgaaaaag aaggtaattt tagtagacca taatgaaagg agccagtcta
      961 ttgatggatt ggaagaagca gaaatattag agataataga tcatcacagg gtggcagatg
     1021 tatttacagg aagtcctatt tattttagaa atgaaccagt aggcagtacc tgtaccataa
     1081 ttgcttctat aatgtttgaa aatggtagaa gaccatccaa aaaaatagct ggtatcctat
     1141 ctgccggtat aatttctgac acacttttgt ttaaatcacc tacatcaacc aagacagata
     1201 ggattatgct agaaaggctg gctagaatag cagatttaga tgtagaagag tttgcagcag
     1261 aaatgtttaa ggcaggtaca tctttaacag ggaagacacc tgatgaatta ttaaaccaag
     1321 attttaaatc cttcaatatt aatgagaaaa aggtaggaat agcccaagtc tatactatgg
     1381 atttagaaag tttagatgga atgaaggata gcctgattca actaatgcag gaaagggcca
     1441 gagaatacaa ttactccata tttatgttaa tgcttacaga catatttagg gaggcatcag
     1501 aaatgatagc agttgggaaa aataaggagc tggtaggcca ggcctttgga aaagaattaa
     1561 ttaacaactc cttgtatgca cctggagtcc tatcaagaaa aaaacaggtt gtgccaccta
     1621 ttacaaatac cttaacaaat attcaagaga tgttgagata aaaaaagaga gaccaagtct
     1681 ctctttttat tatttt
//
LOCUS       DATGSI010000671         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_566432, whole genome shotgun sequence.
ACCESSION   DATGSI010000671 DATGSI010000000
VERSION     DATGSI010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566432"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /gene="thrC"
                     /locus_tag="VK031_10195"
     CDS             complement(<1..1049)
                     /gene="thrC"
                     /locus_tag="VK031_10195"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HLS54336.1"
                     /translation="MEYISTRSNREPIKGSQAILRGISPDGGLYLPKALPLIDNLEDL
                     LSLNYRQMALYVLKKFFPELGKERLKNAVNKAYDEKFSAGPVKIRRTKQVNFLELYHG
                     PTLAFKDMALALLPHLLKASKDLENIKEDIIILTATSGDTGKAALEGFANVEGVKIIV
                     FYPKEGVSQVQELQMVTQEGKNTLVVGVKGNFDDTQSGVKTIFNDVDFKERLRENNYI
                     FSSANSINIGRLVPQIIYYIYAYCQMVNKGDISLGERINIVVPTGNFGNILAAYYAKR
                     MGLPVNKLICASNDNNVLTEFLNKRRYDIRRDLILSSSPSMDILISSNLERLIFHLNQ
                     KDHQLVKDKMDELEI"
     gene            complement(1063..2274)
                     /locus_tag="VK031_10200"
     CDS             complement(1063..2274)
                     /locus_tag="VK031_10200"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HLS54337.1"
                     /translation="MLGIAILGLGTVGQGLVQIINDREKEIEKLLGKKIEIRRVLVKD
                     KGKERDIKLPEGILTDDFNEIIRDEKIQVVIEVTSGLDQVYPYVKSSIEQGKHIITAN
                     KAIVSKYFEELSQLAEEKQVAFLYEASVAGGIPVIKALKEHIALNEINEIQGILNGTC
                     NYILSRMTKEGLDYEKVLKKAQELGYAEADPKADVEGHDTLRKLRIISTLALQGKVDE
                     EDILLEGIERIKAVDIRLVERLDAVIKLIGEGACYEDGFTALVMPAIVRNDSYFSNVA
                     GAFNSVAFNGSNVGELKFYGPGAGKLPTANAVLTDLLDIGLNNFTVRNPLGDRKLKNN
                     NDIIKGKYYLRISQENKKLKDKLKTISEKILNEGKDFAIISREVKLREVYNILKEFQL
                     EKQDYFIARFL"
BASE COUNT          633 a          536 c          303 g          833 t
ORIGIN      
        1 ttaatttcta attcatccat cttatcctta actagttgat ggtctttttg gtttaaatgg
       61 aagattaacc tttctaggtt actggagatt agtatatcca tagaaggcga tgaacttaaa
      121 attaagtccc ttcttatgtc ataccttctt ttatttaaaa attctgttaa aacattatta
      181 tcattggagg cacaaataag cttatttacc ggtagcccca tcctttttgc ataataggca
      241 gccaatatat taccaaagtt accggtggga acaacaatat ttatcctttc accaaggcta
      301 atgtccccct tattaaccat ctgacaatag gcatatatat aatatataat ctgcggaacc
      361 agcctaccaa tatttataga attggctgat gaaaatatat agttgttttc ccttaatctt
      421 tctttaaagt ccacgtcatt aaatatggtc tttactccac tttgggtgtc atcaaagtta
      481 ccctttactc caactactaa ggtgttttta ccttcctggg taaccatttg aagctcttgg
      541 acctggctga ccccctcctt gggataaaaa acaataatct ttaccccttc cacattggca
      601 aagccctcca gggcagcctt gcctgtatca cctgatgtgg ctgttaatat gattatatcc
      661 tcctttatat tttccaaatc cttactagcc tttagaagat gaggtaataa ggccaaggcc
      721 atatccttaa aggccaaggt aggcccatgg tataattcta gaaagtttac ctgttttgtc
      781 cttcttatct taactggccc agcactgaac ttttcatcat aggccttatt tactgcattc
      841 ttaagtcttt ccttacctag ttctgggaaa aactttttta aaacatataa ggccatctgc
      901 ctgtaattta aagaaagcaa atcctctaga ttatctatca gtggcaaagc ctttggtaga
      961 taaaggcctc catctggaga tattcctctt aatatggcct ggctaccctt aataggctcc
     1021 ctattacttc ttgtacttat atattccata atctccaccc cactataaga atctagctat
     1081 aaaataatcc tgtttttcaa gttggaattc ttttaaaata ttatatactt ccctaagctt
     1141 tacctccctg ctaataatag caaaatcttt cccctcatta aggatttttt ctgaaatagt
     1201 ctttaatttg tcttttagct ttttattctc ctgggatatt cgcaaataat attttccttt
     1261 tattatatca ttattatttt taagtttcct atctcccaaa gggtttctca ccgtaaagtt
     1321 attcaaacct atatctaaaa gatctgttag gactgcattg gctgtaggca acttcccggc
     1381 cccaggtcca taaaatttaa gctctcccac attacttcca ttaaaggcaa ctgaattaaa
     1441 agctccagcc acattggaaa aataagaatc atttctaact atggctggca taactaaggc
     1501 tgtaaatcca tcttcataac aggcaccttc tcctattaat ttgatcacag catctaacct
     1561 ttctactaac cttatatcta cagcctttat cctttctatc ccttccagta aaatatcctc
     1621 ttcatcaacc tttccctgta aggccagggt gcttataatc ctaagtttcc gtaaggtatc
     1681 atgaccttcc acatctgctt ttggatccgc ctctgcatag cctaattctt gagccttttt
     1741 tagcaccttc tcataatcta ggccttcttt agtcatccta gataatatat aattacaagt
     1801 tccatttaag atcccctgaa tttcatttat ctcatttaga gctatatgct cctttaaggc
     1861 ctttattact ggaatcccac ctgcaacgct ggcctcatat agaaaagcta cctgcttttc
     1921 ttctgccagc tgacttaatt cctcaaaata tttagatact atggccttgt tggctgtaat
     1981 tatatgtttt ccctgctcta tagaactttt tacatatgga tagacctgat ccaatccact
     2041 ggttacctct attactacct gtatcttttc atcccttatt atttcattaa agtcgtctgt
     2101 taaaattcct tcaggtagct taatatctct ttccttcccc ttatccttta ctagcaccct
     2161 tctgatttct attttcttgc ctaataactt ttcaatttct ttttccctat cattaattat
     2221 ttgaactaga ccctggccta ctgtacctag gcctaatatt gctataccca gcataagtct
     2281 ccctcctaaa gttctttggc tacca
//
LOCUS       DATGSI010000672         1058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_43023, whole genome shotgun sequence.
ACCESSION   DATGSI010000672 DATGSI010000000
VERSION     DATGSI010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43023"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1058
                     /locus_tag="VK031_10205"
     CDS             <1..>1058
                     /locus_tag="VK031_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLS54338.1"
                     /translation="VVSTVAVIYNSFNISVIERISQFGLLRSVGATPKQIRRIVLKEA
                     AILSVISIPIGLVSGVFAMKIVFYIIGLIQSEADLLNEMEITFSSSVFLISTIIGLIT
                     VFLSAIGPARQAARVSPLEAVRNTKDIKKESIKKVRGSRLIRKVLGVEGEIAHKNLRR
                     NRKRFIITVFSMVISIILFITFSTFSDFTFEIGAIDSSRSSDFEIYGNLGDRADDIYT
                     SLMDMEDVERVYRIQESGGSSLIDRKKINEKLIDMNPHMLDGDKGDIIDVYNSSLAMI
                     GDENLEVLNKHLKSGKIDIDKMNKENGVLVINKTYSYKGMTDVRVLLEGFNLKVGDKV
                     PFASYDHDVIDEDTKYTD"
BASE COUNT          380 a          156 c          224 g          298 t
ORIGIN      
        1 gtagtatcta cagtggcagt aatatataac tcctttaata tttcagtaat agaaaggatc
       61 tcccagtttg gcctacttcg ctctgtaggg gcaacaccta agcaaattag aagaattgtc
      121 ctaaaggaag cagctatcct gtcagttata agtataccaa ttggattggt atctggcgta
      181 tttgctatga aaatagtctt ttatataata gggcttattc agtcagaggc agatttatta
      241 aatgaaatgg aaataacttt ttcaagcagc gtatttttaa taagcaccat aatcggatta
      301 ataacagtat ttttatcagc cataggtcca gccaggcagg cagcaagggt atcacccttg
      361 gaagctgtaa gaaatacaaa agacataaag aaggaatcta ttaaaaaggt tagaggctcc
      421 aggttaatta ggaaggtact aggggtggaa ggggaaatag cccacaaaaa cctcaggaga
      481 aacagaaaaa gatttataat cacagtattt tccatggtaa taagtattat tcttttcatt
      541 accttctcta ccttttcaga ctttaccttt gaaatagggg ctatagatag ttccagatca
      601 agcgattttg aaatttatgg caacctggga gatagggcag atgatatata tactagtcta
      661 atggacatgg aagatgtgga gcgggtgtat agaatccaag aatctggtgg ttcaagccta
      721 atcgatagaa agaagataaa tgaaaaactt atagatatga atcctcatat gctagatgga
      781 gacaagggtg acataattga tgtttataat tctagcctag ccatgatagg ggatgaaaac
      841 ctggaagtat taaataaaca tttaaaatca ggcaagatag atatagataa aatgaataaa
      901 gaaaatgggg tcctggtaat taacaagacc tattcctaca agggaatgac tgatgtacga
      961 gtacttttag aaggatttaa cctaaaggta ggtgacaagg tcccctttgc ttcctatgat
     1021 catgatgtaa tagatgagga cacaaaatat acagattt
//
LOCUS       DATGSI010000673         1261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_707277, whole genome shotgun sequence.
ACCESSION   DATGSI010000673 DATGSI010000000
VERSION     DATGSI010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707277"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VK031_10210"
     CDS             <1..411
                     /locus_tag="VK031_10210"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HLS54339.1"
                     /translation="NLLDAIDKTDQLPKTILYCLNASDNDVLGTMIGNFQDGRTPGKL
                     QFGTAWWFHDQKDGIEKQMISLANLGLFRRFVGMVTDSRSFLSYTRHEYFRRVLCNLV
                     GTWAENGEVPYDMDLLGGMIKEICYENAVKYFGI"
     gene            598..756
                     /locus_tag="VK031_10215"
     CDS             598..756
                     /locus_tag="VK031_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54340.1"
                     /translation="MFYAFFINGIAALVIEAVMASILVDFGLGYDRVGMLLSIQSVEN
                     LVASFMEG"
     gene            1067..>1261
                     /locus_tag="VK031_10220"
     CDS             1067..>1261
                     /locus_tag="VK031_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54341.1"
                     /translation="MAVLMVLTYMNMDIGNEKKYEGSWKKEFDNSLAFLSNVDFNLSC
                     AILLFYVGVEYAVNGWMVTYL"
BASE COUNT          382 a          179 c          303 g          397 t
ORIGIN      
        1 aatctattgg atgcgattga taaaacggat caactaccaa agactatcct ttactgccta
       61 aatgctagtg acaatgatgt tttaggtacc atgattggaa acttccaaga tggaaggacc
      121 ccgggtaaac tacaatttgg gacagcctgg tggttccatg accaaaaaga tggcatagaa
      181 aagcaaatga tctccttggc aaacctgggg ctatttagaa ggtttgtagg tatggtaaca
      241 gactctagaa gtttcttatc ttatacgagg cacgagtact ttagaagagt gctttgcaac
      301 ttggtaggta cttgggctga aaatggagag gtaccttatg atatggacct attaggtggt
      361 atgataaaag aaatatgcta tgaaaatgca gtaaagtatt tcggaatata agaagtacta
      421 aaataagaat gggggaggca actagtgagg ggagttccct tccggtatgg accttcctca
      481 attctgcaat aagtaaaaac aagatttaat tgtatacaaa acgtggaaat tttcctggta
      541 cttgtcttga tgagggatag gatatatgga tggtcggggt aaaaagattt taatatcttg
      601 ttttatgcct tctttataaa tggaatagca gccctagtaa tagaggcggt aatggcatca
      661 attttggtag actttggttt agggtatgat agggtcggaa tgctactatc aattcaatct
      721 gtagaaaacc tggtagcaag cttcatggag ggataatatc gggatacacg ggtaggaaaa
      781 atgctatagc aatcttgtca tccatgactg ctctaggttt atgggcttaa ccataatcga
      841 gtctccagct ttcttatttt acttttttcc taacaggtat aggtagaggt agtgttacta
      901 aaatgagtaa tacaattgta aatgaagtag gagatggaaa acctggacca ctcaacatcg
      961 tacatatgtt ctttgtttta ggaacattta tgagcccgct ttttacatct tgggcctttg
     1021 aaatgtgatt cgatgggact cttttgtatt gtggggagtt tctggattgg ctgtgctgat
     1081 ggtcttaacc tatatgaata tggatattgg aaatgagaag aaatatgagg gaagctggaa
     1141 gaaagagttt gataactctt tggctttttt aagtaatgtt gattttaatt tatcgtgtgc
     1201 aattctactc ttctatgttg gagttgaata tgctgtgaat gggtggatgg tcacctatct
     1261 a
//
LOCUS       DATGSI010000674         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_660150, whole genome shotgun sequence.
ACCESSION   DATGSI010000674 DATGSI010000000
VERSION     DATGSI010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660150"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1902)
                     /locus_tag="VK031_10225"
     CDS             complement(292..1902)
                     /locus_tag="VK031_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HLS54342.1"
                     /translation="MKEKFGRWHILFIEYLKRDWKKIIIWILGLGLFSGGFIPAFEEI
                     TKDGGLLGMYETLQNPAMISIVGPTPITKANDYTLGAMYAHEMLLFCSLFAMTISILH
                     VIGHTRKEEDLGLTEIIRSFPIGRQANSLAVIGETVAINLLLSIFIAGVMMSFNVATI
                     SAKGAVLFALSISMSGIMGASIALFMSQVMPLSSAATGASLGLMGFLYVIRGITDISN
                     VNLSILNPLGWTYLTYPFTENNFTPISFALVFSLVLSIAAFVLEGGRDMGAGYLPERE
                     GRERARKSLLSIPGLFIRLNRGVIISWLIAFLILGAAYGAIYGDIESFVHSNEIIQQM
                     FLHSRVSVEESFTATIMMVMVSLVTILPIVIINRLFSEEENFYLSQIYGTKVTRGQLY
                     WTNTILATVAAILGVFFSILGLGGAALAVMKDSSMEFFDFLTIGFNLFPSILFFVGLA
                     ALVLGWIPRLGKVIYIYLAYSSLLNYFEDLLDLPEFILKTAPLSWIPRIPMEDFNTKI
                     FILVTSISILLILLGYSGYKRRDFIEGD"
     gene            complement(1924..>2160)
                     /locus_tag="VK031_10230"
     CDS             complement(1924..>2160)
                     /locus_tag="VK031_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HLS54343.1"
                     /translation="ELRHLTRSTVIVETEKDVALIKDVEGVHDFLKKDNKGIFSADNE
                     HINNILIEATKLGVVKFEAAPPTLEDLFMRHYEG"
BASE COUNT          759 a          438 c          312 g          651 t
ORIGIN      
        1 aacataaaaa gacctttgct tatgcatata aacataagca aaggtcttgc tacctattat
       61 aggttagtta accgggttaa accgtattga cgattaacta attgtaagct actccccttt
      121 gatatctaca ttatatatca gatattaaat catatcaaga taaatattgt ctagttaatt
      181 attaatcttt atcctataat atttaaaatt ttatatctct aaatcatcat acctataatc
      241 aaacttacca tatctggaaa gtacaatcca catttatatc cttatgaagt tttaatcccc
      301 ttctataaaa tcacgtcttt tataaccaga ataacctaat aatattaata gtatactgat
      361 tgaggtgacc aaaataaaaa tcttagtatt aaagtcctcc ataggtattc taggaatcca
      421 gcttagaggt gccgttttta aaataaattc aggcaagtct aacagatcct caaaatagtt
      481 taataaggat gaataggcta gataaatata aattactttc cccaatcttg gtatccaacc
      541 taagactaat gcagcaagac caacgaaaaa taagatagac ggaaataaat taaaaccaat
      601 tgttagaaag tcaaaaaact ccatactact atccttcatt acagcaagtg ctgctcctcc
      661 aagccctaat atggaaaaga aaacacctaa tatagctgct actgtggcca gaatagtatt
      721 agtccagtac agttggcctc gggtaacctt ggtcccatag atctggctta aatagaaatt
      781 ctcttcttca gaaaaaagcc tattaataat aacaattggt aatatggtaa ctaaagaaac
      841 cataaccatc attatggtag ctgtaaaaga ctcttccaca gaaaccctag agtgtaaaaa
      901 catttgttgg attatttcat tactatgaac aaaactttca atatctccat aaatcgcccc
      961 ataagctgct cctaaaataa ggaaggcaat taaccaactt ataatcactc ctctatttaa
     1021 tctaataaag aggccaggta tagatagtag agattttctt gctctttctc tcccctctct
     1081 ctccggtaga tatccagccc ccatatccct acccccttca aggacaaaag ctgctattga
     1141 cagaactaaa ctaaatacta aagcaaagga aattggggta aaattatttt cagtaaaagg
     1201 ataggttaaa taggtccatc ctaagggatt tagaatcgag agattcacat ttgaaatatc
     1261 agttatacct cgtataacat acaaaaagcc cattaatccc aaggaagccc ctgtagcagc
     1321 tgaagaaaga ggcataactt gcgacataaa taaagcaata cttgccccca taattcctga
     1381 cattgatatg gaaagggcaa ataaaactgc tcctttagca gaaatagtgg caacattgaa
     1441 agacatcatc actcctgcga taaaaattga caacagcaaa ttaatagcta ccgtctcccc
     1501 tataacagcc agggagttgg cttgccgtcc tatgggaaaa gagcgaatta tttccgttag
     1561 tcccaagtct tcttccttcc gggtatggcc tattacatgt aagatggata tggtcattgc
     1621 aaataaactg caaaataata gcatttcatg ggcatacatg gcccctaatg tatagtcatt
     1681 ggcctttgta atgggtgtag gccctactat agaaatcata gcaggatttt gtaaagtttc
     1741 atacattcct aaaagccccc cgtcctttgt tatctcttcg aaggccggga taaaaccacc
     1801 agagaatagg cctaaaccta atatccaaat aataattttt ttccagtctc tttttaaata
     1861 ttctataaac aatatatgcc aacggccaaa tttttctttc attataatcc ccccttccaa
     1921 ggactaacct tcataatgac gcataaataa atcttctaaa gttggaggag cagcttcaaa
     1981 ttttactact cctagttttg ttgcctctat caagatatta tttatatgct cattatctgc
     2041 agaaaaaatt cctttattat ccttctttaa aaaatcatgt actccttcta catcctttat
     2101 taaagctaca tctttctctg tttctacaat gacagtggag cgagttaaat ggcgtaattc
//
LOCUS       DATGSI010000675         1605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_92141, whole genome shotgun sequence.
ACCESSION   DATGSI010000675 DATGSI010000000
VERSION     DATGSI010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92141"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VK031_10235"
     CDS             <1..664
                     /locus_tag="VK031_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS54344.1"
                     /translation="RKGEILGISGLMGSGRTELARAIFGADTIDEGEVYLNGKKLDIN
                     NPSDAISEGIGYLSEDRKRDGLALELDVENNIILASIKDFANKFGVIQRSKAIKESEK
                     QVKNLRVRTPSISQKVKNLSGGNQQKVIIGKWLCKDMDVLIFDEPTRGIDVGAKFEVY
                     TLMNDLARSGVGIIMISSELPEILGMSDRVLVMNEGKIAAELSIEEADQETIMFYATG
                     GK"
     gene            699..>1605
                     /locus_tag="VK031_10240"
     CDS             699..>1605
                     /locus_tag="VK031_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLS54345.1"
                     /translation="MGKIEKELNLKEADKKKGIKGISRDSIQRIAALLSLVALFIFFS
                     IAAAPNFLVPQNLINIMMQTTVTGILSLGMTFVLITGGIDLSIGSLIAFSGVMTAVSI
                     GYGASPVIGVIVGILSGVVLGIISGLLISKGKLPPFIASLGMMMFARGLTLLITEGTP
                     LYFQDAPNFKYFSQGKLGAVPYPVIYFIALTILSHFILTKTSIGRYIFATGSNEEAAR
                     LSGIPVDRIKTFVYIYSGLFAAIAGIILAARLNSAQPTVGEGYELDAVAAAVIGGTSL
                     VGGEGTIAGTIIGAFIMSVLKNGLNL"
BASE COUNT          543 a          195 c          356 g          511 t
ORIGIN      
        1 aagaaagggc gaaatcctag gtatatcagg ccttatgggg tcagggagga cagaattagc
       61 aagggccata tttggagctg ataccattga tgaaggtgaa gtatatctaa atggtaaaaa
      121 gttggatatc aataatccta gtgatgcaat atctgaggga attggttatt tatctgaaga
      181 tagaaagaga gatggcctag ctttagagtt ggatgtagag aataatataa tccttgctag
      241 tataaaggat tttgctaata aatttggagt aatccaaagg tctaaggcca taaaagaaag
      301 tgaaaaacaa gttaaaaatt taagggtaag gactcctagt atatctcaaa aggttaaaaa
      361 tcttagtggt ggtaatcaac aaaaggttat tataggaaaa tggctatgta aggatatgga
      421 tgtacttatc tttgatgagc caacccgtgg aatagatgtt ggggctaaat ttgaagtcta
      481 cactttaatg aatgatttgg ccagaagtgg agtaggtatt ataatgattt cttctgaatt
      541 gccagagatt ttaggcatga gtgatagagt tttagtaatg aatgaaggca aaatagctgc
      601 tgaattaagt atagaagagg cggatcagga aactattatg ttctatgcaa caggtggaaa
      661 ataaatcaaa agtatatttt aaaaaaggaa gtgtttttat gggaaagata gagaaggaat
      721 tgaatttaaa agaagcagac aaaaagaaag gtataaaagg aattagtaga gattcaatac
      781 aaagaattgc tgctttattg agtcttgtgg ctttatttat atttttctca atagcagctg
      841 cacctaattt cttagtacca caaaacttaa ttaatattat gatgcaaaca actgtaacgg
      901 gaatattatc cctaggtatg acttttgttt taattactgg cggtattgat ttatctatag
      961 gatctttaat agccttttct ggagtaatga ctgctgtttc cataggttat ggggcctcac
     1021 cagtaatagg agttatagta ggaattttat caggagtagt tttgggaatt atttcaggat
     1081 tattaatttc taaggggaaa ttacctcctt tcatagcatc actaggtatg atgatgtttg
     1141 ctaggggttt gaccctatta attactgaag gaacaccttt atactttcaa gacgcaccaa
     1201 attttaaata tttttctcaa ggtaaattgg gggctgttcc ctatccggta atttacttta
     1261 ttgctttaac tatattaagt cattttattc taactaagac ttccatagga agatatatat
     1321 ttgccacagg aagtaatgaa gaggcagcaa gattatcggg aattcctgta gataggataa
     1381 agacttttgt atatatttac tcaggtttat ttgctgccat agctggtatt atattagctg
     1441 ctagattaaa ctcagctcaa cccacagtag gggagggata tgagttggat gctgtagctg
     1501 cagctgtaat aggtggaact agtcttgtag gtggagaagg aacaatagca ggtacaatta
     1561 taggtgcctt tattatgagt gttcttaaaa atggtctaaa tttaa
//
LOCUS       DATGSI010000676         2845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_707447, whole genome shotgun sequence.
ACCESSION   DATGSI010000676 DATGSI010000000
VERSION     DATGSI010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707447"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VK031_10245"
     CDS             <1..647
                     /locus_tag="VK031_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLS54346.1"
                     /translation="EGTGVCDISLRFIDKNFREVNHKHSLINPERQISPASTGIHGIY
                     DKDVENSPTLGDFFKFNNEFKKGTNKVYIVGHNVRYDWEFIKDYLECEYELVDTLVLA
                     KRYFQGAENYKLQTIRVMAGVHLDLSDQHSASGDTKTLLNILRVIGKNTGKDALQLCR
                     ESNKALPTDPMPIGKYRGQPIGEIVKNDPEYMGWMYNNAERLDQKVKDLIRILL"
     gene            663..833
                     /locus_tag="VK031_10250"
     CDS             663..833
                     /locus_tag="VK031_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54347.1"
                     /translation="MWVLTIILFLAGGDSTVIGDISSTKEECVAKAKYALSVEDPRVV
                     LIKAYCSKAKEV"
     gene            833..2479
                     /locus_tag="VK031_10255"
     CDS             833..2479
                     /locus_tag="VK031_10255"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HLS54348.1"
                     /translation="MAVCVEKLPHSCGTRDGLQVFEEEGKYTGYCFSCDTYIRNPYED
                     KPEDYKPNVIIKSEKDVEKEIEAISDYTTTDLPERGLRKDSLEYFNIKIGLSEEDGET
                     PVTHYYPYYDPSEKLTGYKVRLIEGKKMWVVGSIKDSKPFGWKQAVTAGGKKLFICEG
                     EMDAVALYQICKDFNAGTRYEKFDPAIISVKNGASSATKDLTDNLNEIRKIWDEIVLV
                     FDQDDAGKSAVEKVLRVIPDAKVATLPGKDVNDCLLKGRSRAVYQAVQFNASKPKNTR
                     LVNASTLFQDAKKAPEWGVPWPWKHITETTRGIRKGETIYIGAGQKMGKSEVVNALAA
                     HFITELDWKVMLAKPEEANVKTVKLVAGKIAGKSFHDPKVEFDEKAYDEAVEKIGSNL
                     LLINLYQHLGWDTLQADIREAAQEGCDAIFIDPITNLVNGMSASDQNTKLQEIAQELS
                     AMALDLDVVIFIFCHLRNPDSGDPHDRGGEVLSSQFAGSRAMARSCNLMLGIEGNKDP
                     ELSVEERNIRTLVLLEDREYGEVGRFKLYWDRHTTLFNEC"
     gene            2415..>2845
                     /locus_tag="VK031_10260"
     CDS             2415..>2845
                     /locus_tag="VK031_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54349.1"
                     /translation="MVKSEGLNYTGTDTQHYSTSVKQKEDKYWNDFISKHYNDNKSDL
                     VKRFWYLGHGAEDVVHDAYERVLRYKDSYLGEDFDRWFNTILYNAMRDYRKFILGRDD
                     DDIDEYSHVGAECKGIDSTLMHEVNKLIQTKNPKHQEVLKL"
BASE COUNT          999 a          438 c          645 g          763 t
ORIGIN      
        1 aagagggaac gggtgtctgt gatatttccc ttcgattcat cgataaaaat tttagagaag
       61 taaaccacaa acatagttta ataaatcccg aaagacaaat atcacctgcg tctacaggaa
      121 tacatggcat atatgacaaa gatgtagaga acagccccac tctaggtgat ttctttaagt
      181 ttaataatga gtttaaaaaa ggaaccaaca aggtttatat tgtaggccat aacgtaaggt
      241 acgattggga atttataaaa gattatctag aatgcgagta tgagttggta gatacattgg
      301 tactggccaa gaggtacttc caaggggcag agaactataa attacaaact attagagtaa
      361 tggcaggggt tcacctagac ttgtctgacc agcactctgc tagtggagac actaagactc
      421 ttttaaatat acttagggtt attggtaaaa acactgggaa agacgcttta cagctttgca
      481 gagaatctaa taaagcccta cccactgatc ctatgccaat aggtaaatat aggggtcagc
      541 ctataggaga gattgtaaaa aatgatcctg aatatatggg gtggatgtac aacaatgcag
      601 aaagattaga tcaaaaggtt aaagacctta taaggatttt attataaact aaggagattg
      661 tcgtgtgggt attaacaatt attttgttcc tagccggagg agattccaca gttattgggg
      721 atatttcttc tactaaagaa gagtgtgtag ctaaagctaa atacgctttg agtgtagaag
      781 accctagagt agttttaatt aaagcttatt gctcaaaagc taaggaggtt tagtggccgt
      841 atgtgtagag aaactaccgc actcttgcgg aactagagat gggcttcagg tattcgaaga
      901 agaaggtaaa tacacaggat attgtttctc atgcgacaca tatattcgca atccttacga
      961 agacaaacct gaagactata aacctaatgt aattattaaa agtgagaaag atgtagaaaa
     1021 agagatagaa gctatttctg actatactac taccgatctc ccagaaagag gcctaagaaa
     1081 agactcccta gaatatttca atattaaaat agggctgtca gaagaagacg gagaaactcc
     1141 ggtaacacat tactaccctt attacgaccc ttcagagaaa ctaacaggat ataaagttag
     1201 acttatcgag gggaagaaaa tgtgggtggt ggggtctata aaagactcta aaccttttgg
     1261 ttggaaacag gctgttactg ctggaggtaa gaaactattt atctgtgaag gagagatgga
     1321 tgcggtagct ttatatcaga tatgtaagga ttttaatgct ggaactaggt atgagaaatt
     1381 tgatccagcc attattagtg taaagaacgg agcctcttct gcaactaaag atcttacaga
     1441 taatcttaat gaaataagaa agatatggga tgaaatagtt ttagtattcg atcaagacga
     1501 tgctggtaaa agtgcagttg aaaaggtact aagggttatc cctgatgcta aggtagcaac
     1561 ccttccaggt aaagacgtta atgattgttt gcttaaaggt cgatctaggg ctgtttacca
     1621 ggcagttcag ttcaacgcat ctaaacccaa gaatactagg ctagttaatg ctagtacgct
     1681 gtttcaggac gctaagaaag cccctgagtg gggagttccc tggccttgga aacatatcac
     1741 agaaaccacc aggggcatta gaaaagggga gactatttat ataggggcag ggcaaaaaat
     1801 gggaaaaagt gaagtggtta atgccttagc agcccacttc ataacagaac tagattggaa
     1861 agtcatgtta gccaaacctg aagaggctaa tgtaaagacc gttaaattgg tagcaggaaa
     1921 aatagctgga aaatcttttc atgaccctaa ggtagaattc gatgagaaag catatgatga
     1981 ggctgttgaa aaaataggaa gcaatttact attaattaat ttataccaac acctagggtg
     2041 ggatacttta caggcagaca tcagagaagc agcccaagaa ggatgcgatg ctattttcat
     2101 agaccctatt actaatttgg tcaatggtat gtctgcatcc gatcagaata ctaagctgca
     2161 agaaattgcc caggaactta gcgctatggc tttagattta gatgtagtta tatttatctt
     2221 ttgccatcta agaaacccag attcaggaga tcctcatgac aggggaggag aagtattatc
     2281 ttctcagttc gcaggatcta gggctatggc tagaagttgt aatcttatgc taggaatcga
     2341 aggtaataaa gatccagaac ttagtgtaga agaacgtaat atcagaactt tagttttatt
     2401 agaggataga gaatatggtg aagtcggaag gtttaaatta tactgggaca gacacacaac
     2461 attattcaac gagtgttaag caaaaagagg ataaatattg gaacgacttt atctccaaac
     2521 attataacga caataagtct gatttagtaa aacggttctg gtacttaggc catggggctg
     2581 aagatgtagt acacgatgct tatgagaggg tattaagata taaggatagt tatttgggag
     2641 aagattttga ccgatggttt aacactattc tgtacaacgc tatgcgagac tatagaaaat
     2701 ttatcttagg aagagatgac gatgacatcg atgagtatag tcatgtagga gcagagtgta
     2761 aagggataga ttctacccta atgcatgagg ttaataagtt aatacaaaca aaaaacccta
     2821 agcatcaaga ggtattaaaa ctctt
//
LOCUS       DATGSI010000677         1164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_43253, whole genome shotgun sequence.
ACCESSION   DATGSI010000677 DATGSI010000000
VERSION     DATGSI010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43253"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VK031_10265"
     CDS             complement(<1..284)
                     /locus_tag="VK031_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HLS54350.1"
                     /translation="MKDSSIVRLLKLVKPYKGIVILSGIFALLVNGAELLKPYILKVV
                     IDDFLVLNKAESGLYSIKAMGLLYMGATVIGSLLTYGQVNLMNYVGQEIVT"
     gene            complement(274..>1164)
                     /locus_tag="VK031_10270"
     CDS             complement(274..>1164)
                     /locus_tag="VK031_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS54351.1"
                     /translation="ITFIGRVINITEKGMASYKRLDEVFQEKPDIQSFYEDKSLTRVS
                     GDIEFKGLNFSYPFSKNLALKNINLKIPEGTTLGIIGETASGKTTLVNLLLRLYKVDR
                     GKITIGKRDINDYPVEVLRESIGYVPQDNILFTTTIRESIHFSRDIYTDEEIEEATKL
                     SHIYENIVEFPRGFDTIVGERGVNLSGGQKQRISIARALIKDPKILILDDSLSAVDTK
                     TEEGIINNLKEVLKDKTGIIIGHRISVVQDADHIIVMEEGEIVEEGSHDELLAKEGVY
                     YDIYKSQFEEVAGELVTNEG"
BASE COUNT          326 a          264 c          168 g          406 t
ORIGIN      
        1 gtaactattt cctgcccaac atagttcatt agattaacct ggccataggt taataaggac
       61 ccaattacag tggcccccat atacaatagg cccatggcct taattgaata aaggccagat
      121 tcagccttat ttaagactag aaagtcatca attacaacct taagaatata aggttttaaa
      181 agttcagccc cattaacaag tagggcaaag atacctgata aaattactat tcccttatag
      241 ggttttacca gcttaagcaa cctcactata ctgctatcct tcattggtca ccagctcccc
      301 tgccacttct tcaaattgac tcttgtaaat atcataatag actccctctt tggctaataa
      361 ctcatcatgg ctaccttcct ctacaatttc cccctcttcc ataacaatta tatgatcagc
      421 atcttgtact actgagatgc gatgaccaat tataattcct gttttgtcct ttaagacttc
      481 ttttaaattg ttaataatac cttcctcagt ttttgtatcc actgcagata gagaatcatc
      541 cagaataagg atttttggat cctttatcag ggccctggca atggatatcc tttgcttttg
      601 tcctccagat agatttactc cccgctctcc tactatggtg tcaaaacctc taggaaattc
      661 aacaatattt tcatatatat ggctcagttt tgtggcttct tctatttctt catctgtata
      721 aatatccctg gaaaaatgga tactttccct aatagttgtg gtaaataata tattgtcctg
      781 aggtacatag ccaatacttt cccttaatac ctccactgga taatcgttta tatccctctt
      841 acctatggtg attttacccc tatccacctt atataatctt aatagaaggt tgaccaaggt
      901 agtctttcca ctggctgtct ctcctataat accaagtgtg gttccttccg ggatctttag
      961 gttaatattt ttcagggcta agtttttgct aaagggatat gaaaaattta ggcccttaaa
     1021 ctctatatca ccacttactc ttgttagaga tttgtcctca tagaagcttt ggatatctgg
     1081 tttttcctgg aaaacttcat ctaacctctt gtaagaggcc atcccttttt ccgttatatt
     1141 tattaccctt ccaatgaatg taat
//
LOCUS       DATGSI010000678         1124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_660524, whole genome shotgun sequence.
ACCESSION   DATGSI010000678 DATGSI010000000
VERSION     DATGSI010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660524"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VK031_10275"
     CDS             <1..611
                     /locus_tag="VK031_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HLS54352.1"
                     /translation="MKYFKYGKKETKYLKQQDPILGKAIDEIGMVYREINSNIFEALV
                     SSIISQQISTKAAITVKARLVKLLGQISPEKINSVSIDDIQKCGMSMRKADYIKGIAD
                     AALSGTIDFDSLHKLSDIDVTKKLTSLRGVGQWTAEMLLIHSLERPDIVSYKDLGIRR
                     GIMKLYELEELPKKQFQVYKKRYSPYGTVASIYLWEISGRKD"
     gene            770..943
                     /locus_tag="VK031_10280"
                     /pseudo
     CDS             770..943
                     /locus_tag="VK031_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
BASE COUNT          410 a          144 c          264 g          306 t
ORIGIN      
        1 ggatgaaata ttttaaatat gggaaaaaag aaactaaata cttaaagcaa caagatccta
       61 tactgggtaa ggcaatagat gaaataggga tggtctatag ggaaattaac tctaatatat
      121 tcgaggctct agtatctagc ataataagcc agcagatttc taccaaggca gctattactg
      181 ttaaagctag gttagtaaaa ttactgggcc aaattagtcc ggaaaagatt aatagtgtca
      241 gtattgacga tatacaaaaa tgtggtatgt ccatgagaaa ggcagactat attaaaggaa
      301 tagcagatgc tgccctgtct ggaacaatag attttgatag cctacataaa cttagtgata
      361 tagatgttac aaaaaaatta acatccctta gaggtgtggg acagtggact gcagaaatgc
      421 ttctgattca ttctttggaa aggccagata tagtgagcta taaggactta ggtattcgaa
      481 gggggataat gaagctgtat gaactggaag aactaccaaa gaaacagttt caagtataca
      541 aaaaaagata tagtccttat ggaacagttg cctccatata tttatgggag atatctggga
      601 gaaaggatta ggcttggtaa atttgaagta gaatttggga aaaacttggc caaagattta
      661 aaaaggctag gaataaatag ggtttagtgg aagggatgtc caactgttta ggatggctag
      721 gtctatccag aacctatatg ccagtgaaat agcttagaaa acatatctta aggttgatga
      781 ggaagggact gaagccagct ccattgtgaa ggtagagata agtagggaat gtgcacctat
      841 tggagataat atggatttta acaggccttt tatctatgca attgtggatt tggagaataa
      901 tttacctata tttatgggag taatggataa tccggttgaa taggtggcgg taaaaactgg
      961 aaagattcat aataatccat tgacaaagga ttattaactg gagaaacaat gacggttcaa
     1021 ggaggtagtg gtctcagacc ataaatataa gattttcctc aagtaaaggc ctcattgaca
     1081 gaatatttcc aagggtatac aattacatta aagtgtgaaa gggg
//
LOCUS       DATGSI010000679         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_92425, whole genome shotgun sequence.
ACCESSION   DATGSI010000679 DATGSI010000000
VERSION     DATGSI010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92425"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..274
                     /locus_tag="VK031_10285"
     CDS             35..274
                     /locus_tag="VK031_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54353.1"
                     /translation="MKSYIFEGKKNSYCSNKECGFSLFKKDRFFTDKRKKLTKEMVKV
                     SLSKGGVTVKGLCSKKTGKTYNAYISIEDTGKYIN"
     gene            394..1104
                     /locus_tag="VK031_10290"
     CDS             394..1104
                     /locus_tag="VK031_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HLS54354.1"
                     /translation="MDNYILETRNLCKFYKNQKAVNNISISVKRNSIYGLLGPNGAGK
                     STFLKMIAGILHPTSGQIIFDNQIWSRKDLANIGALIESPPLYENLTARENLKVRSIL
                     LGIQDTRIDEVLKIVGLTNTGRKKASQFSMGMKQRLGIAIALLNNPKLLILDEPTNGL
                     DPIGIQELRDLIKTFPSRGITVILASHILSEVKQLADHIGIINNGILGYQGEVDANVD
                     LESVFMEVVVSTQQEGVQ"
     gene            1101..1832
                     /locus_tag="VK031_10295"
     CDS             1101..1832
                     /locus_tag="VK031_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HLS54355.1"
                     /translation="MIKYLKAENLKCKRSFTKKIIILSPIITLLIALLSPLWFEVNGY
                     NFWYVLMLPGTITLLAILINQKEEKKLFYRGVYSLPVDLKKIWYGKLFLISIYLLLSN
                     MLLVIGIKAIGTIFNLPLRISFIKGFIAIIIMLITSFWQIPFSLFLCNKFNLLWGILI
                     NFGLGTVLNIVLVDSSIWWISPYSYTSRLMAVLLGIHPNGTPLEPGDLLINFSLVPII
                     IGISLILAVGFAILTANWFQNQEAK"
     gene            1834..>2476
                     /locus_tag="VK031_10300"
     CDS             1834..>2476
                     /locus_tag="VK031_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HLS54356.1"
                     /translation="MLMLWRSIKADFIKLKRTSITYIHILIPIIGASLFLFYYSISSW
                     STETKLAAYYHAMAIIFPLLIGIITGIITDQEEGAGGFQNMFTILKGKATSYLSKLFV
                     LLLLSVFSIIMAILTFALVFKNYSLEFYTSLGIALFLGNIPLYILHLFISFRFGKGAS
                     IGLGFAGALISALMETGLGDGIWQFIPWAWGIRFGDFLMLKELNQLTFPYIFEL"
BASE COUNT          854 a          342 c          443 g          837 t
ORIGIN      
        1 aatcaaatag ataagtatta gggagttgcc caaggtgaag agctacatct ttgaaggaaa
       61 gaagaattct tattgttcca acaaagaatg tggcttttct ttatttaaaa aggatagatt
      121 ctttacggat aaaagaaaga aactaactaa agaaatggta aaggtttcat tatcaaaagg
      181 aggagtaacg gtaaaaggat tgtgttcaaa aaagacagga aagacctata atgcttatat
      241 ttctattgaa gatacaggta aatatattaa ttagaaaatg aggttttaat aattactaga
      301 tatattttta aaaattgatc tattaattaa atccttataa aatccttaaa attatttatt
      361 acaattaagc taataaaaag aaaggatgat tatatggata attatatttt ggaaacaaga
      421 aatctctgca agttttataa aaatcaaaaa gctgtaaaca acatatctat ttcagtgaaa
      481 agaaattcta tttacggcct acttgggcct aatggagctg ggaaatctac atttttaaag
      541 atgattgctg gaattcttca tccaacttca ggacagatta tttttgataa tcaaatatgg
      601 agtagaaagg atttagctaa tataggagca ttaatagaat cacccccact atatgaaaac
      661 ttaactgcca gagaaaattt aaaggttaga tctattttac ttgggattca agatactaga
      721 attgatgagg tgctcaaaat agtaggctta acaaatacgg ggagaaagaa agcatctcag
      781 ttttcaatgg ggatgaaaca gagactagga attgccatag cccttttaaa taatccaaaa
      841 ttattaattt tagacgaacc aacaaatggc cttgacccta ttggaattca ggaactaagg
      901 gatttgatta aaacctttcc ttcccgaggc ataacagtta tactagctag ccatatttta
      961 tctgaggtaa aacagctggc agatcatatt gggataataa ataatggtat tttaggatac
     1021 cagggagaag tagatgccaa tgtagattta gaatctgtgt ttatggaggt agtggtaagt
     1081 acacaacagg agggagtgca atgattaaat atttaaaggc tgagaactta aaatgcaaaa
     1141 gaagctttac taaaaaaata attatactaa gccctattat caccctatta attgcactcc
     1201 tatcaccttt gtggtttgag gtaaacggat ataatttttg gtatgtgctt atgttacctg
     1261 gaacaataac attgttagcc atactaatta accaaaaaga ggagaagaaa ttattctata
     1321 ggggggtata ttctctacct gtagatttga aaaaaatatg gtatggaaaa ttgtttttaa
     1381 tttctatata tctactttta tcaaatatgc tactggtaat aggtataaaa gctataggaa
     1441 ctatatttaa tcttcccctg agaatttcat ttatcaaagg ctttatcgct ataattatta
     1501 tgttaattac atctttttgg cagattccat tttccttatt tctatgtaat aagtttaacc
     1561 ttttatgggg tatcttaatt aattttggcc tgggaacagt attaaatatt gtgttggtag
     1621 attctagtat atggtggatt tctccatata gctatacttc tcgtttaatg gctgtccttt
     1681 taggaatcca cccaaatggt accccactag agccaggaga tttattgata aactttagcc
     1741 ttgttccaat tattatagga atttccctta tactagctgt aggctttgca atactaacgg
     1801 ctaattggtt tcaaaatcag gaggcgaaat aatatgttaa tgttgtggag aagtataaaa
     1861 gcagatttta taaagctaaa aaggacctca atcacatata ttcatatatt aattccaata
     1921 ataggcgctt ccctatttct cttttattat tccatatcca gctggagcac tgaaacaaag
     1981 ttagctgcat attatcatgc aatggctatt atatttcccc tattaattgg aataataaca
     2041 ggcataatta cagaccagga ggagggggct gggggttttc aaaatatgtt tactattctc
     2101 aaggggaagg ctacatccta tttaagtaag ctttttgttt tacttttatt atctgttttt
     2161 tcaattataa tggccatcct tactttcgcc cttgtattta aaaactatag tctagaattt
     2221 tatacaagct tagggattgc acttttttta ggaaatatac ccttatacat tttacaccta
     2281 tttattagtt ttaggtttgg gaaaggggcc tccataggtt tagggtttgc tggagcatta
     2341 atatcggcat taatggaaac aggtcttggc gatggaatat ggcagtttat tccttgggct
     2401 tggggaatta gatttggtga ttttttaatg ctaaaggaat tgaatcaatt aactttccct
     2461 tatatatttg aactta
//
LOCUS       DATGSI010000680         8532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_375323, whole genome shotgun sequence.
ACCESSION   DATGSI010000680 DATGSI010000000
VERSION     DATGSI010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375323"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VK031_10305"
     CDS             <1..263
                     /locus_tag="VK031_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HLS54357.1"
                     /translation="ICAGPVVLERAGIIKGKKITSYPGFEKELEGAKYQASPIVVDGN
                     IITARGPYYAVNFAISIIDYLLGKEKAEELKEDILYNEILEG"
     gene            335..868
                     /locus_tag="VK031_10310"
     CDS             335..868
                     /locus_tag="VK031_10310"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HLS54358.1"
                     /translation="MILIFAVDKSWAIGFDGKMLVEIREDLKRFRKLTEGNIVIMGRR
                     TLEAIPGRNPLPNRINILVTRSDKYNNKGFYIIDDLKKLKPLLKELNPEGDKKVFVTG
                     GESIVKQLLPYCNRAYITKILKDFPKTDTNIPNLDLDNSWIIVDESPIYYQDDLGYKY
                     VEYKRKNKETPFYLNQV"
     gene            complement(888..1163)
                     /locus_tag="VK031_10315"
     CDS             complement(888..1163)
                     /locus_tag="VK031_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HLS54359.1"
                     /translation="MKLIVLNGRRIHSKEEFHKEIKDKFDFPSYYGANLDALWDMLTT
                     INHHTTIRLINHKLLVEKLGPYGEKILDLLQDLVGENKKINLILDPK"
     gene            complement(1173..1646)
                     /locus_tag="VK031_10320"
     CDS             complement(1173..1646)
                     /locus_tag="VK031_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HLS54360.1"
                     /translation="MKQRLLSLVLVLSILVGLFTGCTPAIEKISDNNTEQLSENRQVQ
                     VDEEGIYTSPEEVALYIHHFNQLPKNYITKEEARSLGWESSKGNLWEVTDQMTIGGDK
                     FGNREGLLPQAEGRQYFECDVNYEGGFRGPERLVYSNDGLIFYTQDHYNTFQRLY"
     gene            complement(1706..2335)
                     /locus_tag="VK031_10325"
     CDS             complement(1706..2335)
                     /locus_tag="VK031_10325"
                     /inference="COORDINATES: protein motif:HMM:NF015866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HLS54361.1"
                     /translation="MSILKEACVGSFTEAKKAWQLGADRIELCDNLAEGGTTPSMGTI
                     KMAKEVLDIPICVIVRPRGGSFVYTHEEIQIMEEDIKRCKQMGADGIVIGALDENNEL
                     NLEVLERLIGQAGDMELVFHMAFNLIEDKKAALDKLIDLGINRVLTSGGEGSAFDNME
                     NLKELIEYAKDRITILPGGGITEDNYMELVEKTGAKEVHGTKIVGSLSR"
     gene            complement(2361..2966)
                     /locus_tag="VK031_10330"
     CDS             complement(2361..2966)
                     /locus_tag="VK031_10330"
                     /inference="COORDINATES: protein motif:HMM:NF025536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4309 domain-containing protein"
                     /protein_id="HLS54362.1"
                     /translation="MIGEVITIKNLNLLIIMIVISLVLTSCSLGVREKDLPQEDIVEP
                     GSGSGGKEGEPNDSNDIKDEDKDHGHFSDQDYNILKNLEESARILDMDKDKVVDSLGQ
                     PEEIDYYGAEYYKYEDRIIFFEQDDQVSSIWWTDLEDLFNIPGRLTNEKVKKYLGKPK
                     FEGIDEIEPDEYYLHYQVDNFSIRFINLDNESGYVHIKDLS"
     gene            3077..4096
                     /locus_tag="VK031_10335"
     CDS             3077..4096
                     /locus_tag="VK031_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HLS54363.1"
                     /translation="MEKVVVVGAGVMGSGLAIHLANKGKRVNLWGTRWDRNLIKEMQD
                     SKQHKDFQVDLPNTINLYYHNQLEEAFKGVRLVIIAVVSKGVANICRKITPYLKDDHI
                     ILSVSKGIDETSLLTMSSLIRQTLPVHLREKVDIVKLGGPIIAAELANLKYTEAVFAS
                     HNLNSAKYVGEMFKTDKFKINISTDIEGVDLCSALKNTYAIGMGMMEGLERGSNNPQA
                     ALMARGSLEMGRIVKAYGGEILTALSIAGVGDYYVTSLGGRNGQFGKYLGKGNTIERA
                     LELMEGQTVEGLAGTLNAYRLLEKLEGEGRFNIEKEAPLFNEIYKVLYEGKLASEAMA
                     DYWKR"
     gene            complement(4138..4683)
                     /locus_tag="VK031_10340"
     CDS             complement(4138..4683)
                     /locus_tag="VK031_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54364.1"
                     /translation="MKTYKVFFIFGLLSILITGCSPILVKEEPYRLVPNEVSTSQIKT
                     RSVGENLAVEKELAINERLKETIEILNMNKADVYEKFGEPKLIDYFQVEYFKYKDKII
                     FFSHEKVSSIWWTKLDDLFGIEGEVTIEKVESVFGPAYWEGYLDSEIIIGHCLSYGIG
                     NLQINFANLDTENAFVTVNYK"
     gene            5336..5971
                     /locus_tag="VK031_10345"
     CDS             5336..5971
                     /locus_tag="VK031_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54365.1"
                     /translation="MDFRNKKIWIIGIVILALIFGLFYQNMRIKRYDRILRAYDHNSI
                     NTYKYLMAKTNFKSSIERLEDLYELDEIDDEMLIEISDIFGKITDHLMHLNVLRGYSI
                     QDDMLKENESRITDYSYILLYKEQSESDEIINKIYLGFEELLMDMYQTGIEEDHKKTI
                     EELTANLRQIEGLMKSSVNRHIDPNDLGELREEYVRIEPMLNTIVEILDKN"
     gene            6185..6745
                     /locus_tag="VK031_10350"
     CDS             6185..6745
                     /locus_tag="VK031_10350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HLS54366.1"
                     /translation="MLFFIIPTLYIKRCNRYKKRYFGKISKKQAIRSIALGIFVYIFI
                     IGFYLVIKNFIDLNHIQTILVKNLNVNRSNFIFVAFYIAFVNSLLEEYFFRGFIFLSL
                     LKIHERKSAYIISAFLFAIYHVAIMANWFNIYLFILALIGLFIAGILFNWLDEGSKNI
                     YNSWLVHMFANFAINTVGFIMFGFFK"
     gene            6932..7819
                     /locus_tag="VK031_10355"
     CDS             6932..7819
                     /locus_tag="VK031_10355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLS54367.1"
                     /translation="MSKLLDMKKTYKYILLGVVLVMVLGVTACSSGNKEVVAKVNDQV
                     ITKDELYEHMLKQNGTQVLEALIAEKIVDEEAKKEGIEPSKKEIEEEIEKIKEESGGE
                     QGFQQLLQYYGYELKDLEANITMNLKIKGLVGDDIEISDDEIKDYFDKNRENFEQEEQ
                     VRARHILVDTEEEVKEVQEKLAQGEDFEELAKEYSKDTSAQMGGDLGFFHRGEMIKEF
                     EEVAFSLEIGKISEPVKSDFGYHIIRVDERKEAKEADFAEHKDLIREILFEDKLPAVY
                     QEWYQAKYQEYNITNKLAK"
     gene            7948..>8532
                     /gene="wrbA"
                     /locus_tag="VK031_10360"
     CDS             7948..>8532
                     /gene="wrbA"
                     /locus_tag="VK031_10360"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HLS54368.1"
                     /translation="MSKVKLAIVYYSMGGTNYQLAKWAEKGALEAGAEVRIVKARELA
                     AESTIEANPIWKAHVEETKDIKVATSEDLNWADAVIFSTPTRFGNVAAQMKQFLDEQG
                     GIWAKGQLVNKVVSAMSSAQNSHGGQEATIKAIYTTMMHWGAIIVPPGYSDESIFKAG
                     GNPYGASVTVGKEGMVEDVEEAVKHQARRTVQVAQ"
BASE COUNT         2965 a         1328 c         1610 g         2629 t
ORIGIN      
        1 ccatttgtgc agggcctgta gtcttggaaa gggcaggtat tattaagggc aaaaagatta
       61 catcctatcc aggctttgaa aaggagttag aaggggctaa ataccaggct agtccaatag
      121 tggtagatgg taacattatt acagctagag gaccctatta cgcagtaaat tttgctatat
      181 caataataga ctacctgcta ggtaaggaaa aggcggaaga gctaaaggaa gatatactat
      241 ataatgaaat tttggaagga taaatagcca aggataatcc ttggccattt atccatatat
      301 tgatgaaatt tagttagata gggaggagtc agtattgata ctaatttttg ctgtagataa
      361 atcctgggct atagggtttg atggaaaaat gttagttgaa attagggagg acttaaaaag
      421 gtttagaaaa ttaacagaag gcaacatagt tataatggga agaaggaccc tggaggcaat
      481 accaggtaga aatcctttac ctaataggat aaatatactg gttactagaa gtgataagta
      541 caataataaa ggtttttaca taatagatga tttaaaaaaa ttaaagcctt tactaaagga
      601 gctaaatcca gagggggaca aaaaagtctt tgtaactgga ggagaaagca ttgtaaaaca
      661 gctacttccc tattgtaata gggcctatat tacaaagatt ttaaaggact ttcctaagac
      721 agatactaat ataccaaact tagacttgga taatagttgg ataatagtag atgaatcccc
      781 aatatattac caggatgatt taggttacaa atatgtagaa tataaaagga aaaacaaaga
      841 aacacctttc tatttgaatc aagtatgatt caagaaaggt gttttttcta tttaggatcc
      901 aggattaagt ttattttttt attttcacct accagatcct gtaaaaggtc taatattttt
      961 tccccatagg gacctaattt ttcaactaag agcttatgat taattaacct aatggtggta
     1021 tggtggttaa tggttgttaa catatcccat agggcgtcta gattagcccc atagtagctg
     1081 ggaaaatcaa acttgtcttt aatctcctta tgaaattctt ctttactgtg gattcttctt
     1141 ccatttaata caataagttt catacccatc tcctaatata atctttggaa agtattgtaa
     1201 tgatcctgtg tataaaaaat caaaccatca ttggaatata ctagcctttc aggcccccta
     1261 aatccacctt catagttaac atcacattca aaatattgtc ttccctctgc ctgaggtaga
     1321 aggccctccc tgttgccaaa tttgtctcca cctatagtca tctgatcggt aacctcccaa
     1381 agattacctt tactactttc ccaacctagg gatctggctt cctcttttgt aatgtaattt
     1441 ttagggagtt gattaaagtg gtgaatatat agggccacct cttcagggct ggtataaatt
     1501 ccctcttcat ctacttgaac ctgcctattt tctgacagtt gttcagtatt attatctgat
     1561 attttctcta ttgcaggtgt gcatccagta aaaagtccaa ctaaaataga taggaccagt
     1621 actagggata ataatctttg tttcataaaa tatctcctat actaattatt tattaaccat
     1681 taggcctata taaagcgaca atttgttacc tggataaact acctacaatt ttagtgccgt
     1741 gaacctcttt agctccagtc ttttccacca gctccatata attgtcttca gtaataccac
     1801 caccaggcag gatggtaatc ctatctttgg catactctat caattctttt aaattttcca
     1861 tattatcaaa ggctgaaccc tctcctccgc tggtaagaac cctatttatc cctaagtcta
     1921 taagtttatc caaggcagcc tttttatcct caattaggtt aaaggccata tgaaaaacaa
     1981 gctccatatc tccagcctgg cctattagcc tttctaaaac ctctaaattt agttcattgt
     2041 tctcatctaa ggcaccgatt actatgccgt cagcccccat ttgtttacat cttttaatat
     2101 cttcttccat aatctgaatt tcctcatggg tatagacaaa gctacctccc ctggggcgaa
     2161 cgataacaca tataggaata tctaaaactt ccttagccat tttaatagtc cccatactgg
     2221 gagtagtccc cccttcagcc aagttatcac ataattcaat cctatcagca cctaactgcc
     2281 aggccttttt tgcctctgtg aaacttccta cacaggcttc ctttaagata gacataaaga
     2341 tctccccctc aacttaacat ttatgataaa tcttttatat gaacatagcc tgactcatta
     2401 tctaagttaa tgaatctaat ggaaaaatta tctacttgat aatgtagata gtactcatca
     2461 ggctctattt catctatacc ctcaaattta ggcttaccta aatatttttt aaccttttca
     2521 ttagttaatc ttcccggtat attaaataaa tcctctaaat cggtccacca gatactagat
     2581 acctgatcat cctgctcaaa aaatattatt ctatcttcat atttatagta ttctgcacca
     2641 taataatcta tttcttcagg ctgaccaagg gaatctacaa ccttatcctt atccatatct
     2701 aaaattcttg ctgattcctc taaattttta aggatattat aatcctggtc actaaaatgg
     2761 ccatggtcct tatcttcatc ttttatatca ttagaatcgt taggctcccc ttcttttcca
     2821 ccgcttccac ttcctggttc cactatatct tcttgaggaa gatctttctc ccttactccc
     2881 aagctgcagg aagttagaac cagggatatc actatcataa taattaataa gtttagattt
     2941 tttatggtaa ttacctcccc aatcaatatt agcctcatta taacataaga aggttaaatt
     3001 agtttacaag aaaattacaa agactctggt aatatggtag aatatagata aataacagct
     3061 attgggagtg aaaagagtgg aaaaggttgt agttgttgga gcaggagtta tgggcagcgg
     3121 tctggcaatt catctagcaa ataaggggaa aagggtaaac ctatggggga ccagatggga
     3181 tagaaaccta attaaagaaa tgcaggatag taagcagcat aaggacttcc aggtagatct
     3241 acctaatact ataaacctat actaccataa tcaattggaa gaggccttta aaggagttag
     3301 attggtaata attgcagtag tttcaaaagg agtagcaaat atttgtagaa aaattactcc
     3361 ctacttaaag gatgatcata ttatcctaag tgtttctaag ggaatagatg aaaccagctt
     3421 acttaccatg tcttcactta ttagacagac cttacctgtc cacttaaggg aaaaggtgga
     3481 tatagtaaag cttggtggac ccataattgc agcagagctg gcaaacttaa agtatactga
     3541 agcagttttt gcctctcaca atttaaatag tgccaaatat gtgggggaaa tgttcaaaac
     3601 agataagttt aaaataaaca tatctaccga tatagaaggg gtcgacctat gttctgcact
     3661 gaaaaacacc tatgccatag gtatgggtat gatggagggg ttagaaagag gtagcaacaa
     3721 tccacaggca gccctaatgg caagaggtag ccttgaaatg ggaaggattg taaaggccta
     3781 cggtggagag atattgacag ccctatccat tgcaggggta ggtgattact atgtaacctc
     3841 cttaggtgga agaaacggtc aatttggaaa atatttaggt aaggggaaca ccattgaaag
     3901 ggccctggag ttgatggaag ggcaaactgt ggaaggattg gccggcacct taaatgctta
     3961 taggctattg gaaaaactag aaggagaagg cagatttaat atagaaaaag aggctcccct
     4021 atttaatgaa atttataagg ttctttatga ggggaagttg gcaagtgaag caatggctga
     4081 ttactggaaa agataaggat ggggcttgtc cccatccttt ttatataaag atttcattta
     4141 cttatagttt accgtaacaa aggcattttc cgtatctaga ttggcaaagt taatttgtaa
     4201 attcccgatc ccatagctga ggcagtggcc tataataatc tcactatcta aatatccttc
     4261 ccagtaggct gggccaaaaa cactttctac cttttctata gtcacttcac cttcaatccc
     4321 aaacaaatca tctaatttag tccaccaaat actggatact ttctcatggg aaaaaaatat
     4381 aatcttatcc ttatatttaa aatattctac ctgaaaataa tctattaact tgggctcacc
     4441 aaatttctca taaacatctg ccttgttcat atttagaatc tctatagttt ccttaagtct
     4501 ttcgtttatg gctaactctt tttcaactgc taagttttca ccaacacttc tagttttaat
     4561 ctgggaggtg gaaacttcat taggtaccag cctatatggt tcttccttaa caagtatagg
     4621 actacatcct gttattaaaa tggataaaag gccaaatata aaaaagactt tataagtttt
     4681 cattcctatt ccccctatca ttaagcctgt cttagtatta tagtcaaaag ggaaaaattt
     4741 atacttaatt atttggaata gattagctaa aatcattaac catataagtt aatctttaag
     4801 gagaaattat ctccataatt cgaccttaaa taagattttt tgaaaaatca tttctaagta
     4861 ggaagaaaca aggaaagata aataaatagt ataagtaaat gtttaatttt tttaaaaaag
     4921 aaaagttctt taagtttact ttaagttttt aataactaag actttatttt cctctccaag
     4981 taaagataag aaaacaggct aggaactgcg gaaataagag gatcttagat aattacagaa
     5041 aaagttggat aaaaagaatt acaaaaaact taaagacaag ctttaacttt tcactttaag
     5101 ttttctgatc aggctaagcc ttgataagtt ttataaattt agtttaagtt caaacattaa
     5161 tcaggtataa aaggaatttt cctagggtta ggaatgggga aaatatattt ataggtctta
     5221 ttgacagaaa tccttacatt attccccttt ttttgcaaat ttcatatata atatttataa
     5281 gagaggatcc gggaaattag gaagaatact aaaggaaata aggggagatg cagatatgga
     5341 ttttagaaat aaaaagatat ggattattgg gattgtaatt ttagcactta tttttgggtt
     5401 attttatcaa aatatgagga taaaaagata cgataggatt ttaagggcct atgatcataa
     5461 ttctattaat acttataaat atttaatggc aaagaccaat ttcaagtcca gcatagagag
     5521 gttagaagac ttatatgagt tggatgagat agatgatgaa atgttgatag aaatatcaga
     5581 tatatttggg aaaattactg atcatttaat gcatctaaat gtattaagag gctattccat
     5641 ccaggatgat atgttaaaag aaaatgaatc tcgaataaca gattactcat atattttgtt
     5701 atacaaggaa caaagtgaat ccgatgagat aataaataaa atttatttag ggtttgagga
     5761 attgttaatg gatatgtatc aaacaggaat tgaggaggac cacaaaaaaa ctatagaaga
     5821 gctaactgca aacttacgtc aaattgaagg tttgatgaag tcttcagtaa acagacacat
     5881 tgaccctaat gatttgggag aacttaggga ggaatacgtc aggatagaac ctatgttaaa
     5941 cactatagta gaaatattag acaaaaatta acctagcttt aaagaggcta aaaaatggcc
     6001 tctttagttt tttgaagatc ttgtggcagc aaaatgacaa gaatggtttg gggtgggaaa
     6061 tatgacaaaa gacaacaagg acaagttaac aatttatttt ataataactt tgtcgatttt
     6121 tcttatgttt ataatcgact atattataca ggctaaatat atatggaaat cactaataaa
     6181 aattttgttg ttttttataa tacctacgct ttatataaaa agatgcaata gatataaaaa
     6241 aagatatttt ggtaaaatat ctaaaaagca ggccataaga tccatagcct tagggatatt
     6301 tgtttatata tttattatag gtttttatct tgtcataaaa aactttatag atttaaacca
     6361 tatccagacc atactagtaa aaaatttaaa tgtaaacagg agtaatttta tttttgtagc
     6421 cttttatata gcctttgtta actctctttt agaagaatat ttttttagag gatttatctt
     6481 tcttagttta cttaaaatac atgaaagaaa aagtgcctac ataatcagcg cctttctctt
     6541 tgccatatat catgtggcca taatggccaa ttggtttaac atatacttat ttattctagc
     6601 cttgataggc ctttttatag ctgggatctt atttaattgg cttgatgaag gaagtaaaaa
     6661 tatatataat tcatggctgg tgcatatgtt tgcaaacttt gcaatcaata ctgtaggatt
     6721 tataatgttt ggttttttta agtaattggg ttttagactg tggctgaaag tattatttat
     6781 atatgagaag aaattaaaaa agatggaata gattagaaaa atcatttcta tcatggaaat
     6841 cctatatatt tagtgtataa tagtagttga atgtttttgt agactatgca gaaataaaag
     6901 ttgcaaatat ggaaggtggg agctttttaa gatgagtaaa ttattagata tgaaaaaaac
     6961 ttacaagtac atcctattag gtgtggtttt agtcatggtg ctaggagtta cagcatgtag
     7021 ttcaggaaat aaggaagttg tggccaaggt taatgatcag gtaataacta aagatgagtt
     7081 atatgagcat atgttaaaac aaaatggaac tcaagtttta gaggccttga ttgctgaaaa
     7141 aatagtagat gaagaggcta aaaaggaagg aattgagcca tctaagaagg agattgaaga
     7201 agaaatcgaa aagatcaagg aagaaagtgg tggagaacaa ggcttccaac aactacttca
     7261 atattatgga tatgaactaa aggatctaga agcaaatata accatgaact taaagattaa
     7321 gggcctggtt ggagatgata ttgaaataag tgatgatgaa ataaaagatt actttgataa
     7381 aaatagggaa aactttgaac aggaagagca ggtaagggct agacatatcc tggtagatac
     7441 agaagaggaa gtaaaagagg ttcaagaaaa attagcccaa ggtgaagact ttgaagaatt
     7501 ggcaaaggaa tattcaaagg atacttcagc ccaaatgggt ggggatctag gcttctttca
     7561 taggggcgaa atgattaagg aatttgaaga agtggccttt agcctagaaa taggaaaaat
     7621 tagtgaacca gtaaagtctg attttggcta ccatataatc agggtagacg aaaggaagga
     7681 agcaaaagaa gcagattttg cagagcataa ggaccttata agggagatcc tttttgagga
     7741 taagttgcca gcggtttacc aagaatggta tcaagccaag taccaagagt ataatataac
     7801 aaacaagttg gcaaaataaa tattaagata taaatactca gctgtaaagg ttgagtattt
     7861 tttattgtaa atatagatta cttcaggact ttgggggtat actaaaagtg ataaagattt
     7921 gttaacttat ggaagggaga tttatatatg agtaaagtaa agttggccat agtttactat
     7981 agtatgggtg gaaccaatta tcagctggct aaatgggcag aaaaaggagc cttggaggct
     8041 ggggcagagg ttaggatagt taaagccagg gagttagcgg cagagtcaac catagaagcc
     8101 aatcctatct ggaaggccca tgtggaggaa actaaggata taaaggtggc aacatctgag
     8161 gacttaaact gggcagatgc tgttatattc tccacaccta ctcgctttgg caatgtggca
     8221 gcccagatga aacagttttt agatgagcag ggcggtattt gggccaaggg acaactggta
     8281 aacaaggtgg tttcagccat gtcttcagca cagaattcac atggtggtca ggaagccaca
     8341 ataaaggcta tctacaccac tatgatgcat tggggagcta ttatagtacc accaggatat
     8401 tcagatgaat ccatatttaa ggcaggtgga aacccttacg gtgctagtgt tacagtaggt
     8461 aaagaaggca tggtagaaga tgtggaggag gctgtaaaac accaggctag aagaactgtt
     8521 caggtagccc ag
//
LOCUS       DATGSI010000681         1314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_707621, whole genome shotgun sequence.
ACCESSION   DATGSI010000681 DATGSI010000000
VERSION     DATGSI010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707621"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..>1314)
                     /locus_tag="VK031_10365"
     CDS             complement(8..>1314)
                     /locus_tag="VK031_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLS54369.1"
                     /translation="EATYGKIFQRALGQFDKFNLHNIIKVRPVDDLNLEIVFDKVYSN
                     NLEVLTFPIVASHRLKGQGKATYTEALREENYKPIGTGPYKFVDYERFKNVYLEKNPD
                     YWKGSPYIDKIVGKVLADNELILNAFETGQINLAISQGVDWAKYRQNKRIEVLEFISG
                     DYEFLGFNFKNKLLGSQKGQAIRQAINYAIDRQDIIERVYLKHATQIDVPIHPDSYLI
                     SKYAHSYGHNKEMAKEILKKAGFTNQDKDGTLIDEEGNKLSFTMITNSFNPLRIKTME
                     IIREDLNKVGIDIKIASPIYYNEDELTDEFIDKEWAKMEDRVKKGDFDIVLLGWNTSV
                     ITDLTPMFHSQAIKAGDNFISYENEKMDQLLERAYFSSREGKEKAFESLQELIVEDLP
                     YLSLVFRNRGLLIDKKIKGDLNPNFYNLYKGLENCYIPKDLQ"
BASE COUNT          342 a          275 c          206 g          491 t
ORIGIN      
        1 gtcacaatta ttgtaaatcc ttagggatat aacagttttc aagcccttta tagaggttat
       61 aaaagtttgg atttaaatct ccttttatct ttttatctat taaaagtccc ctatttctaa
      121 aaactaagct aaggtatggc aaatcttcta ctatcagctc ctgtaggctt tcaaaggcct
      181 tttcctttcc ttccctacta ctaaaataag ccctttctag taattgatcc attttttcat
      241 tttcataaga gataaagtta tctccagcct ttatggcctg gctatgaaac ataggagtta
      301 ggtctgtaat tactgaagta ttccagccta gcaaaactat gtcaaagtct ccttttttaa
      361 ccctatcctc catttttgcc cattccttat ctataaattc atctgttaat tcatcttcat
      421 tataatagat aggtgaggct atttttatat ctatgcctac cttatttaaa tcttccctta
      481 taatttccat ggtcttgatc ctaagaggat taaaggagtt agtaatcatt gtaaagctga
      541 gcttatttcc ctcttcatct attaaggttc catccttatc ctgatttgta aagccagcct
      601 tctttaagat ttccttggcc atctccttgt tgtggccata gctatgggca tatttgctaa
      661 ttaaatagga atctggatga attggcacat ctatttgggt tgcatgcttt agatataccc
      721 tttctataat atcctgcctg tctatggcat agttaatggc ctgccttatg gcttggccct
      781 tctggctacc tagaagttta tttttaaagt taaagcctaa gaattcataa tcgccactta
      841 taaattctaa aacctctatt ctcttgtttt gcctgtattt ggcccagtcc actccctggg
      901 agatggccag gtttatctgt cctgtttcaa aggcatttaa aataagttca ttgtcggcta
      961 ataccttgcc tactatcttg tctatatatg ggctgccctt ccagtagtct gggtttttct
     1021 caagatagac atttttaaac ctttcataat ctacaaactt gtaaggccca gttcctatag
     1081 gcttatagtt ttcctctctt agggcctcag tataggtggc cttgccttgc ccctttaacc
     1141 tatggctggc tactatggga aaggttaaaa cttccagatt atttgaataa accttgtcaa
     1201 agacaatctc taaatttaga tcatctactg gccttacctt tattatgttg tgtaagttaa
     1261 atttatcaaa ttggcctagg gccctttgga aaatcttacc ataggttgcc tctg
//
LOCUS       DATGSI010000682         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_235888, whole genome shotgun sequence.
ACCESSION   DATGSI010000682 DATGSI010000000
VERSION     DATGSI010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235888"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1854)
                     /locus_tag="VK031_10370"
     CDS             complement(<1..1854)
                     /locus_tag="VK031_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HLS54370.1"
                     /translation="MNDKTLKVLEYKKIIDSLQKKAESHLGREKIKNIKPLTDKKKVE
                     QMLMETEEALSLLIKRGNPPLFGIHNISQEIRRVQLGGFLSPAGLLKIADTLRVSRAL
                     KKYIKETKEEETESFPLIEGLIEGLRVYSHIEKSINRAIIDEEEIADNASTRLKNIRR
                     QIARRNNEIKDKLNSIITSPTYKKHLQDSIVTVREGRYVVPVKQESRGSVPGLVHDIS
                     ASGATVFIEPMAVVDLNNQLRELEIKEREEIEKILADLSNLVATEATGIFANQEKLQN
                     LDFIFAKGKLALEMDATRPLLNDKGYINLKRARHPLLDPKEVVPIDVYLGKEFTSLII
                     TGPNTGGKTVSLKTVGLLTLMAQSGLHIPADFNSELAVFDQVFADIGDEQSIEQSLST
                     FSSHMTNIVDILNRVEDNSLILFDELGAGTDPTEGAALAMAILDYLLKKDVRTIATTH
                     YSQLKLYALTTDKVRNASVEFDVETLRPTFRLLIGVPGKSNAFEISQRLGLQSFIIDY
                     ARELISKENIEFEDVLQAIDKDRRQTEEAREEAEKLRKDIKELKRDLAREKEKTEDMR
                     GQLIREARQEARDILRQAKKEADQIVAELRKASAQVGRERNRKIQQAQDRLK"
     gene            complement(1865..2419)
                     /gene="zapA"
                     /locus_tag="VK031_10375"
     CDS             complement(1865..2419)
                     /gene="zapA"
                     /locus_tag="VK031_10375"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HLS54371.1"
                     /translation="MTEKRRVDVTIDGRSFTIVGTETEEYIKSLANYVDKKIKNLSSK
                     NDKLSQTMSATLAALHIADELHRANNKYNKLEEMAKDPLEKYDEVCEKLNDSTVKIES
                     LEKVCSEYQETILQLTSEKESLLKRLEEHEKTIRNKDKIIEESKKEIKSLQKKNLETQ
                     VELVETKKELAEYLKLLDTETSSY"
     gene            complement(2517..>3339)
                     /locus_tag="VK031_10380"
     CDS             complement(2517..>3339)
                     /locus_tag="VK031_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HLS54372.1"
                     /translation="KSYDKIRLEKDSELRKYIELVNPLGEDFSSMRTTLISNMMELLA
                     RNLNRSVEEVFAFEIGNIFIPKELPVKELPIEKKTLCLGFYGKEDFYYLKETVDTLLK
                     RLGIFNVEYVREENNPTFHPGRTANLLVDGQVLGTIGEIHMDVCENYHINRRVYIGQL
                     DFEQIINLADFEKTYKPLPKYPSTSRDIAIVVDEDVMVGQVEKIILNHGKDLIEDIQL
                     FDIYRGDQIPADKKSLAFSIVYRSYERTLKDKEVNKIQEAIIKDLEDNLNAKLRS"
BASE COUNT          794 a          709 c          498 g         1338 t
ORIGIN      
        1 ttttagcctg tcttgggctt gttggatttt tctatttctt tccctgccta cctgggcaga
       61 tgcctttctt aattctgcca ctatttggtc tgcctctttt ttagcctgcc ttaatatatc
      121 cctagcctct tgcctggcct cccgaatcag ttggcctctc atatcttctg ttttttcttt
      181 ttcccgagcc aggtcccttt taagctcttt aatatccttt ctaagttttt cagcttcttc
      241 tctggcctct tccgtttgcc ttctatcctt atctatggcc tggagtacat cttcaaattc
      301 aatattttcc tttgatataa gttcccttgc ataatctatt ataaaggatt gtaagcctaa
      361 cctttgggat atttcaaagg catttgattt gcctggtact cctattagga gcctgaaggt
      421 aggtcttaag gtttccacat caaattctac agatgcattc cttaccttgt ctgtagttaa
      481 ggcatatagt tttagttggc tatagtgggt tgtggctatg gttcttacat ctttttttag
      541 caaatagtct aatatggcca tggccagggc tgccccttct gtaggatcag tccctgcccc
      601 tagttcgtca aataaaatta agctattatc ctctaccctg tttaatatat ctactatatt
      661 ggtcatatga gaagaaaagg tagaaagact ttgctctata ctttgttcat ctccaatatc
      721 tgcaaaaacc tggtcaaaga ctgctagctc tgaattaaag tctgccggta tatgcagtcc
      781 tgattgggcc attaaggtaa gtagacccac tgtttttaaa cttacagttt ttccacctgt
      841 attaggtcct gttattataa ggctggtaaa ttccttgccc agatatacat ctattgggac
      901 tacttccttt gggtctagaa gcgggtgcct tgccctttta agatttatat aacctttgtc
      961 atttaataag ggcctggttg catccatttc taaggccaat tttcccttgg caaagataaa
     1021 gtctaaattt tgtaactttt cctgattggc aaatatacct gttgcctctg tagctaccag
     1081 attagatagg tcagctagga tcttttcaat ttcttctctt tccttgatct caagctccct
     1141 taattgattg tttaaatcta caactgccat aggctctata aatactgttg ctccactggc
     1201 acttatatca tggaccaggc caggcacact tcccctactt tcttgtttaa ctggcaccac
     1261 atatctacct tctctaactg ttactatgct gtcttgtagg tgttttttgt aggtgggaga
     1321 tgtgattatg gagtttaatt tatcctttat ctcattattt cttctggcta tttgccttct
     1381 tatatttttc aacctagtac ttgcattatc agctatctct tcttcgtcta ttattgccct
     1441 atttatactt ttttctatat gggaatatac tcttaatcct tctatcaaac cttctatcaa
     1501 gggaaaactt tctgtttcct cttcttttgt ttcctttata tattttttta gggccctgga
     1561 cactctcaag gtatctgcta tctttaacag gccagcagga cttaaaaagc ctcccaattg
     1621 gacccgccta atttcctggc ttatattgtg aatcccaaat aggggtgggt tccctctttt
     1681 aattagtagg cttaaagcct cttcagtctc cattaacatc tgttctacct ttttcttatc
     1741 agttaagggt ttgatatttt taattttttc ccttcctaaa tgggattcag cctttttttg
     1801 taggctgtct attatttttt tgtattctag aacctttaag gtcttatcat tcaactactt
     1861 cacctcaata actgctggtt tctgtatcta gcagtttaag atattctgcc aactcttttt
     1921 ttgtttctac caattctacc tgggtttcta agtttttctt ttgcaaactt tttatttctt
     1981 tcttgctttc ttctattatc ttgtccttat ttcttattgt tttttcatgc tcttctaatc
     2041 tttttaacag gctttccttt tcggaagtca gttgcaaaat ggtttcctga tattcagagc
     2101 ataccttctc caaactttct atctttactg ttgaatcatt taatttttca caaacttcat
     2161 cgtatttttc caagggatct ttagccattt cttctaattt attatattta ttattggctc
     2221 tgtgcaattc atctgctatg tgaagcgccg ccaaggtagc agacatggtt tgacttaact
     2281 tgtcattttt cgatgacaga tttttaattt ttttgtctac ataattggcc aaacttttta
     2341 tatattcttc cgtctctgtt ccaactatgg taaagctacg gccatctatt gtaacatcaa
     2401 cccttctttt ctctgtcata actaccctcc catccaatct tatataccta ttatactaca
     2461 aaatctccaa ttagtattaa tatttgaaaa aggagtagat aaatctaccc cttagattaa
     2521 ctccttagct ttgcatttag attatcttcc agatctttga ttatggcctc ttgaattttg
     2581 ttaacttcct tatctttcag tgtcctttca taggacctat atacaatgga gaaggccaag
     2641 ctctttttat ctgctggtat ttgatctccc ctgtatatat caaataattg tatatcttct
     2701 atcaggtctt taccatggtt taagataatc ttttctactt gtcctaccat aacatcttca
     2761 tctacaacta tggctatatc cctagatgtg gatggatatt taggtagtgg tttatatgtt
     2821 ttttcaaaat ctgctaaatt aataatttgt tcaaagtcca actgacctat ataaaccctt
     2881 ctgtttatat ggtagttttc acatacatcc atatggattt ctccaatagt tccaagtact
     2941 tgaccatcta ccaacagatt ggcagttcta cctggatgga aggtagggtt gttttcttct
     3001 ctgacatatt ctacattaaa gataccaagt ctttttagta gtgtatctac tgtttctttt
     3061 aagtaataaa agtcttcctt gccatagaaa cctaaacata gggttttctt ctctattggt
     3121 aattctttta caggtaactc cttaggaata aagatgttac ctatttcaaa ggcaaatact
     3181 tcttctacgc ttctatttaa attcctagct aaaagctcca tcatattgga tattaaggtt
     3241 gttctcatgg agctaaagtc ttcccccagt gggtttacta gctctatata tttccttagc
     3301 tcactgtctt tttcaagcct tatcttgtca taggactta
//
LOCUS       DATGSI010000683         1137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_236022, whole genome shotgun sequence.
ACCESSION   DATGSI010000683 DATGSI010000000
VERSION     DATGSI010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236022"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..>1137
                     /locus_tag="VK031_10385"
     CDS             15..>1137
                     /locus_tag="VK031_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HLS54373.1"
                     /translation="MPKPEIASVGATFSDSEVRHGDAYSHLLELLGLQDEFTKLMEIP
                     CMKERIKYLSAANEKSNSHDPKEYFESLILFSMMVENVSLFSQFFIIMSYNKYGNMLT
                     GMSNVIEATSKEEACFIEGTEILTPEGWVDLRQAHTGMRVYQYNKDGSIEPVTATHAT
                     HKPYKGTMYSFSKRGRQAVVTEDHRITYTNHKGEWKEKLAKDFKGDTKKYIPESGTIF
                     WKGRGMSPWERLLVAFQADGFPSYWTNKEGDRLYRGAAGGKNAVISVKKQRKKDRLEF
                     LLIDCGIDFTTIESKPGYTDYKFYIKDAHKYKDFSWINLDDVGSKWANDFVEELSLWD
                     GSLVEEGVDRKLLYSSTNKSCVDIAQAIGTLAGYRCHISK"
BASE COUNT          379 a          189 c          270 g          299 t
ORIGIN      
        1 atatctacca ccacatgcct aagccagaga ttgctagtgt aggagctaca ttcagcgata
       61 gtgaagtaag acatggggat gcttattctc acctgttaga attactagga ctacaggatg
      121 aattcactaa gctgatggaa attccctgca tgaaggaaag gattaagtac ctttcggcag
      181 ctaatgagaa gtctaattca catgatccta aagaatactt tgaatccctt attctattct
      241 caatgatggt agagaatgtt tctctattca gtcagttctt tatcatcatg tcatataata
      301 aatatgggaa tatgctaact ggtatgagta atgtgattga ggcaaccagc aaggaggaag
      361 cttgttttat agagggtacc gagattctta ctcccgaggg atgggttgat cttagacagg
      421 cgcacactgg tatgagagta tatcagtata ataaagatgg ctctatagag cctgtcacag
      481 ccacacacgc cacacacaaa ccctacaaag gaactatgta ttctttttcc aagagaggga
      541 gacaggctgt cgttacagag gatcatagga taacatacac taaccataag ggagagtgga
      601 aggagaaact agctaaagat ttcaaaggag atactaagaa atatattcca gagagcggga
      661 caatcttttg gaagggaaga ggcatgtccc cttgggagag acttctagtt gcctttcaag
      721 cagatgggtt tccctcttat tggactaata aagaaggaga taggttgtac agaggagcag
      781 cagggggcaa gaatgctgta atatctgtca aaaagcaaag aaagaaggat aggcttgagt
      841 ttttattgat agactgtggt atcgatttta caactataga aagtaagccc ggatacactg
      901 actacaagtt ttatatcaag gatgcccaca agtataaaga cttctcttgg atcaatttag
      961 atgatgtagg aagcaaatgg gctaatgatt tcgtagaaga actcagccta tgggacggtt
     1021 cactggtaga ggaaggggta gatcgaaaat tattatatag ttctactaat aaaagctgtg
     1081 ttgacatagc tcaagccata ggaacattgg cggggtacag atgtcacatt agtaaaa
//
LOCUS       DATGSI010000684         1375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_277501, whole genome shotgun sequence.
ACCESSION   DATGSI010000684 DATGSI010000000
VERSION     DATGSI010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277501"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VK031_10390"
     CDS             <1..1089
                     /locus_tag="VK031_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HLS54374.1"
                     /translation="VSGKLTKDMDLYNSSKERVEKLGGLFVLRGKNQINVDEIKAGDI
                     GATSKLQHTQTGDTLCDRSNVITFDRLSYPQPTLFKAVEPKSKGDEERISSSLQRLTE
                     EDPTFTLERNPETKQLLIGGQGNMQLTVITDKLKNSFGVDIILSTPKVAYRETIKGTS
                     SVQGRHRKQSGGAGQYGDVHIRFEPTTEEFVFEEEVFGGAVPRNFFPAVEKGLRESME
                     SGVLAGYPVVNVKATLFDGSYHPVDSNEMAFKIAASLAFRKGIEQADPVLLEPVVKVE
                     INIPEEYMGDVMGDMNKRRGRILGMEQLDDGSQLVIAEAPHAEMFEYAIDLRSMTQAR
                     GSFTMEFVRYEEVPANIAEEIIEKAKAE"
     gene            1214..>1375
                     /locus_tag="VK031_10395"
     CDS             1214..>1375
                     /locus_tag="VK031_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HLS54375.1"
                     /translation="MMGLSNLIEKFIIDLIKNSQNGIIEIQRNEMAEYFECSPSQINY
                     VLSTRFTPYK"
BASE COUNT          500 a          215 c          301 g          359 t
ORIGIN      
        1 gtatcaggta aattaactaa agatatggat ctatacaatt ccagtaagga aagggttgaa
       61 aaactgggcg gcctatttgt cctaagagga aagaaccaga taaatgtaga tgagattaag
      121 gccggagata ttggagccac ctcaaaacta caacataccc aaacaggtga caccttatgt
      181 gatagaagta atgtcataac ctttgatagg ctatcatatc ctcaaccaac cttgtttaag
      241 gcagttgaac cgaaatctaa gggagatgag gaaagaatta gttcatcatt acaaaggcta
      301 acagaggaag acccaacctt tactttagaa agaaacccag aaactaaaca gttacttatt
      361 ggcggtcagg gcaatatgca actaactgta ataactgata aacttaaaaa tagctttggg
      421 gtagatatta tcctttcaac tccaaaggtt gcctatagag aaactattaa gggcacatca
      481 tctgttcaag gcaggcacag aaaacaatca ggaggtgccg gccagtatgg ggatgttcat
      541 ataagatttg aacctactac agaagaattt gtatttgaag aggaagtatt tggtggagca
      601 gtacccagaa acttcttccc agcagttgaa aagggactta gagaatccat ggaatcaggt
      661 gtgttagcag gatatccagt agtaaatgtt aaagctacat tatttgacgg ttcctatcac
      721 ccggtagatt ctaatgagat ggcatttaaa atagcagctt cattagcctt tagaaagggg
      781 atagaacaag cagacccagt attattagaa ccagttgtaa aagtagagat taatattcct
      841 gaagaatata tgggcgatgt aatgggagat atgaacaaac ggagaggtag aattttaggt
      901 atggaacagt tagatgatgg ttcccagctg gtaatagctg aagcccccca tgctgaaatg
      961 tttgagtatg ccatagactt aaggtccatg acccaggcaa ggggaagctt taccatggaa
     1021 tttgtaaggt atgaagaagt gccagcaaac attgcagaag aaataattga aaaggcaaag
     1081 gcagaataaa ataaaaggga ggtaggttta gcctacttcc ctttagtatt tacaaggagc
     1141 acttgaaatt tatttataaa tatagtatat ttatattgaa ggacaaagaa agtcaaagtc
     1201 aaaggcaggt gttataatgg gcttaagtaa tctgattgaa aagttcatta tagatttaat
     1261 taagaattct caaaatggaa taatagaaat acaaagaaat gaaatggctg agtactttga
     1321 atgttctcca tctcagataa attatgtgtt gtctacccgc tttaccccct acaag
//
LOCUS       DATGSI010000685         1750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_423183, whole genome shotgun sequence.
ACCESSION   DATGSI010000685 DATGSI010000000
VERSION     DATGSI010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423183"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VK031_10400"
     CDS             <1..270
                     /locus_tag="VK031_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HLS54376.1"
                     /translation="LATYGLIASVLYRKLNWNLIISLIFAMLMGRIFAGLAAFLLVLL
                     FAAQLDPFIFVKTATITGLPGIGIQLFLIPTLMYGINRYTTINLD"
     gene            281..1567
                     /locus_tag="VK031_10405"
     CDS             281..1567
                     /locus_tag="VK031_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLS54377.1"
                     /translation="MSILIKNVDLIPMDGEERAIYKTNIYIQDKEITHIGQIREDIEV
                     ERVIDGRNKLAMPGLINAHTHIGMSLLRNYADDLPLHDWLTKAIWPVEANLKAEDVYW
                     GSLLSMVEMIESGTTAFCDMYFFMDEVAKGVVESGMKAVLTRGLIEGDESDRKLHETR
                     NLHKSWDGKGDGRIKVMVAPHAPYTCSPNFLKRTMDLAKELNTGIHIHLSETKKEVED
                     SYKEHGKSPIKHVYDLGLLDFHTIAAHCVHLNEEDMDILLEKDVYPVNNPGSNLKLAS
                     GFAPVDKMLKKGITVALGTDGSSSNNNLNMFEEIYLAAVLNKAVNLDAVSVPAYTAIE
                     MATINGARALGWENETGSIEVGKRADMILIDLDKPHLYPRHNLISAASYGMQASDVDT
                     VIIDGKIVMEKREIKSLDVEKIKYMAEKQAKDLINR"
BASE COUNT          618 a          263 c          371 g          498 t
ORIGIN      
        1 ctagcaactt atgggcttat agcctctgta ttatatagga aacttaattg gaacctgata
       61 atatccctaa tttttgctat gcttatggga agaatatttg caggcctagc tgccttttta
      121 ttggtattat tatttgcagc acaattggac ccctttatct ttgtgaaaac tgccactatt
      181 actggactac caggaatagg gattcagtta tttttgattc ccacattaat gtatgggatc
      241 aacaggtata ccactattaa cttggattag gagggattaa atgagtattt taattaaaaa
      301 tgtggaccta attcctatgg atggggaaga aagggcaata tataagacca atatctatat
      361 acaggataag gaaattaccc atattggaca gattagggaa gatatagaag tagaaagggt
      421 cattgatgga aggaacaaat tagcaatgcc tgggcttatc aatgcccaca cccatatagg
      481 aatgtcactt ttaagaaact atgcagatga tttacccttg catgattggc taaccaaggc
      541 catctggcca gtggaggcta atttaaaggc tgaggatgta tattggggtt cccttttaag
      601 tatggttgaa atgatagagt caggaaccac cgccttttgt gatatgtatt tctttatgga
      661 tgaggtggcc aaaggggtag tagaatctgg tatgaaggct gttttaacta ggggcttaat
      721 agaaggggat gagtcagata gaaagttaca tgaaacaaga aatctacata aatcctggga
      781 tggaaagggg gacgggcgga tcaaggttat ggtggcaccc catgcaccct atacctgtag
      841 ccccaacttt ctaaagagaa ccatggattt agccaaagag ttaaatacag gcattcatat
      901 tcatctatca gaaactaaaa aagaagtgga agactcttat aaagaacatg gtaagtctcc
      961 tataaaacat gtatatgatt taggattact tgattttcat acaatagctg cccattgtgt
     1021 ccatcttaat gaggaggata tggacatact attagaaaaa gatgtttatc cagttaataa
     1081 tccaggcagt aatttaaaac tggccagtgg ctttgcacca gtagataaga tgctaaaaaa
     1141 aggcatcaca gtggccttag gcacagacgg ttcctccagc aataacaatt taaatatgtt
     1201 tgaggagatc tatcttgcag cagtactaaa caaggcagta aacttagacg cagtatcagt
     1261 tccagcttat accgccatag aaatggccac tataaatgga gcaagggccc taggttggga
     1321 aaatgaaaca ggatctatag aagttggtaa aagggcagat atgattctaa tagatttaga
     1381 caaacctcac ctttatccaa gacacaattt aatttctgca gcaagttatg gcatgcaggc
     1441 ttcagatgtg gatacagtta taatagatgg taaaatagta atggaaaaaa gggaaataaa
     1501 aagcttagat gtggaaaaaa tcaaatatat ggcagaaaaa caggccaaag atttaataaa
     1561 tagataaaag ataggcattg gtaaatttac caatgcctat tatatttttt gagaaattta
     1621 gggataattc atataaggtg attgataaat atggaaatat ctaaggactt atatgcaaac
     1681 aaggagaact taaaagagtt atttaataat acatttgatt taaccttata tgaatttgaa
     1741 acattatctg
//
LOCUS       DATGSI010000686         1149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_518914, whole genome shotgun sequence.
ACCESSION   DATGSI010000686 DATGSI010000000
VERSION     DATGSI010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518914"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VK031_10410"
     CDS             complement(<1..550)
                     /locus_tag="VK031_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HLS54378.1"
                     /translation="MAKFSTDVLSYYQGLEFDAVAPSRVDVSHINDYNLQGNFTIETK
                     VKLLSGDNAYCLWKRGNNNNGWWFGIDSTGLVFTTFGRTIYNTPMTNKSEWNHISVVF
                     DGQLVKLFINGIEEYSSSFSGLGTTTRGFRMAARSNNNGGIDGYSNHIQDETRLWNVA
                     RTQQEIQLNMNKELTGNEEGLIA"
     gene            complement(543..>1149)
                     /locus_tag="VK031_10415"
     CDS             complement(543..>1149)
                     /locus_tag="VK031_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HLS54379.1"
                     /translation="VDLNKPIFSDNTSDPFTISTILKLNDTSPHWILTQRTGSVSGPK
                     VSFETFSSNLRFFLEDASGNRIHTQTSIPVLSEYFLVTITYDGSKEVSGVKMYINSVE
                     VVPNIVTSDVLGDTFGISDLNTGIGSVIESNHAYSMRGSINEMQIWNKVLTQREIQLN
                     MNKELTGHEEGLVGYWKFDDLSDPTVVIDSSPNQNHGTIHG"
BASE COUNT          353 a          230 c          155 g          411 t
ORIGIN      
        1 aagctattag accttcctca tttccagtta attctttatt catgtttaat tgtatctctt
       61 gttgagttct agcaacgttc catagtctag tttcatcttg tatatgattg ctatacccat
      121 ctataccccc attgttattt gacctagctg ccattctaaa ccctcttgta gttgttccta
      181 acccggaaaa actactacta tattcttcaa ttccgttaat aaatagttta actagttgtc
      241 catcaaatac aactgatatg tgattccatt ctgacttatt tgtcataggt gtattatata
      301 ttgttctccc aaatgtggta aataccaaac cagtactatc tataccaaac caccacccat
      361 tattattatt tcccctcttc cataaacaat acgcattatc accagataat aattttactt
      421 tagtttctat agtaaagttt ccttgtaagt tataatcatt tatgtgagaa acatctaccc
      481 tactaggagc tacagcatca aactctaacc cttgataata acttaaaaca tctgtgctaa
      541 atttagccat gtatagtacc gtggttttga ttaggtgaag aatcaataac tacagtagga
      601 tctgataaat catcaaattt ccaatatcct actaatcctt cttcatgacc tgttaattct
      661 ttgttcatat ttagttgaat ttctctttga gttaacactt tattccatat ttgcatctcg
      721 tttatcgagc ctctcataga atatgcatga ttgctctcta taacacttcc tatacccgtg
      781 tttaaatcac ttatcccaaa tgtatcacct aaaacatctg atgtaactat attagggact
      841 acttcaacag aatttatata catcttcacc cctgagactt ctttactccc atcatatgta
      901 atagttacta aaaagtactc tgataaaaca gggatagagg tttgcgtgtg tattctattt
      961 cctgaggcat cttcgagaaa aaagcgaagg ttagagctga acgtttcaaa cgatacttta
     1021 ggtcccgata ctgaacctgt gcgttgtgtg agaatccaat gagggctagt atcattaagc
     1081 tttaatatag tagaaattgt gaaaggatca ctagtattat cagaaaatat aggtttattt
     1141 agatctaca
//
LOCUS       DATGSI010000687         1715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_376173, whole genome shotgun sequence.
ACCESSION   DATGSI010000687 DATGSI010000000
VERSION     DATGSI010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376173"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VK031_10420"
     CDS             <1..284
                     /locus_tag="VK031_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54380.1"
                     /translation="TFDMSTMILSIVILGGMGTIRGMVVGSLVLVVLPEMSRSLMDYR
                     FVLYGLVLVFMMRFRPEGLLGWRDTRPYPLSEKIRQSLKANERGVKDEL"
     gene            274..1056
                     /locus_tag="VK031_10425"
     CDS             274..1056
                     /locus_tag="VK031_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS54381.1"
                     /translation="MNYKHLLEVKDLSINFGGIKAVDQFNFHVDKNEIIGLIGPNGAG
                     KTTVFNMLTGVYTPTSGNILWKGKNIEGSLPQDIVKQGISRTFQNIRLFDDMRVIENV
                     LIGSHINIKYNLLDSVFENRRFKEHEEEATQKAIDVLKSLNMEDKIDMYADSLPYGDQ
                     RKVEIARAVATGAELILLDEPAAGMNPYESEELLEFISNIRDLGITILLIEHDMNVVM
                     NVSDRVYVLDNGKLISNGLPEEVAHDPNVIKAYLGGEAFANH"
     gene            1043..>1715
                     /locus_tag="VK031_10430"
     CDS             1043..>1715
                     /locus_tag="VK031_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLS54382.1"
                     /translation="MLTIKNLNTYYGAIHALKGVDLQVNEGEIVSLIGSNGAGKSTLV
                     NTIAGLLKPRQGSIVFQGKDITRAESYQLPKEGISLSPEGREVFGSLTVEENLRLGAY
                     IINDNKQIKESYEMVYELFPRLAERRKQYAGTLSGGEQQMLAIGRSLMSQPKLLLLDE
                     PSLGLAPNIVSQIFELVEEISEQGITILLIEQNAFMALRTSDRAYVLENGKISLEGPS
                     QEVLEN"
BASE COUNT          623 a          260 c          376 g          456 t
ORIGIN      
        1 tcacctttga tatgtctact atgatcctaa gtatagtaat cttaggtggt atgggtacca
       61 ttagaggaat ggtagtaggt tcccttgtgt tggtagtatt accagaaatg tcaagaagtt
      121 taatggacta tagatttgtg ctttacgggc tagtattagt atttatgatg agatttaggc
      181 ctgaaggatt gctaggctgg agagatacca ggccctatcc attaagtgaa aaaataagac
      241 aatcattgaa agctaatgaa aggggtgtta aggatgaact ataaacattt attagaagta
      301 aaggatttat caattaattt cggtggaatc aaggcagtag atcaatttaa ctttcacgta
      361 gataagaatg aaataatagg acttatagga ccaaatggag caggaaaaac aacagtattt
      421 aatatgctaa ctggtgtata tactcctaca tcaggaaata tactctggaa aggcaagaat
      481 atagaaggat ccttacccca agatatagtg aaacaaggta tatcaaggac ctttcaaaat
      541 ataaggctct ttgatgatat gagagttata gaaaatgtat taataggtag tcatattaat
      601 attaaatata accttttaga ttctgtattt gaaaatagaa ggttcaaaga acatgaagaa
      661 gaagctacac aaaaagcaat agatgtatta aaaagcttaa atatggaaga taagattgat
      721 atgtatgcag acagcctacc ctatggtgac caaaggaagg ttgaaattgc cagggcagtg
      781 gctacaggag ctgagttaat cctactggat gagccagcag ccggtatgaa cccttatgaa
      841 tctgaggaac tattagaatt tatatccaat attagggact taggcatcac catactttta
      901 atagagcatg atatgaatgt ggttatgaat gtatcagata gagtatatgt gctagacaat
      961 ggaaaactaa ttagtaatgg tctgccagaa gaagtagccc atgatccaaa tgttataaaa
     1021 gcatacctgg gaggtgaggc atttgctaac cattaaaaac ttaaatacct attacggagc
     1081 aatacatgca ttaaaaggag tagatttaca ggtaaatgaa ggagaaatag taagtctaat
     1141 aggcagtaat ggagctggaa aatcaaccct agtaaataca atagcaggcc tattaaagcc
     1201 tcgccagggt agcatagtct ttcagggtaa ggatattact agggccgaat cctaccaact
     1261 accaaaggaa ggaatcagcc tgtcccctga gggaagggaa gtcttcggta gcttgacagt
     1321 agaggaaaac ttaaggctag gggcctatat aatcaatgac aacaaacaaa tcaaggaatc
     1381 ctatgaaatg gtgtatgaac ttttccctag actagcagaa agaagaaaac aatacgccgg
     1441 aaccttatct ggtggggaac aacagatgtt ggccataggt agatccttaa tgagccagcc
     1501 taaactttta ctattggatg agccatcctt aggcctggca cccaatatag tttcacaaat
     1561 atttgagctg gtagaagaaa tttcagaaca gggaatcaca attcttctaa tagaacaaaa
     1621 tgcctttatg gcacttagaa catctgacag ggcctatgtt ttagaaaatg gaaagatttc
     1681 tctagaaggg ccttcccaag aagtgttgga aaatg
//
LOCUS       DATGSI010000688         1785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_519119, whole genome shotgun sequence.
ACCESSION   DATGSI010000688 DATGSI010000000
VERSION     DATGSI010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519119"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VK031_10435"
     CDS             <1..145
                     /locus_tag="VK031_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HLS54383.1"
                     /translation="EKQEAAMVEAFNDIWKIKEDYNVSMRKAAYLHSVKKVAEVMKLR
                     GWY"
     gene            314..901
                     /locus_tag="VK031_10440"
     CDS             314..901
                     /locus_tag="VK031_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54384.1"
                     /translation="MKLGSISEIRTGMILSRKRADSEFEKAETYKLIAIKNVDEEGLF
                     NNEPFEDFDSNDKLDQVYFTKEGDILFRLSHPYTALYIGKLYENLLIPSHFAKIRIKD
                     HRYLPEYVAWYLNREKIKRELLRYQTGSVVPTTNIRILSSLRIKAMDKAKQESIINYN
                     KLYLREKSLLKRLMKENERLNKAITNKLIGGKDND"
     gene            894..>1785
                     /locus_tag="VK031_10445"
     CDS             894..>1785
                     /locus_tag="VK031_10445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HLS54385.1"
                     /translation="MTKKTTQTEINQVLWEASDTFRGKIDSSIYKDYVLVMLFVKYLS
                     DVYAERVEYFHERYEGNQERIERALSRERFILSEKTSFDYLYSKRHDPEIGEIINKAL
                     DKIESDNAGKLRGVFRNIDFNSETVLGETRDRNAMLRTLLEDFNKLDLRPSKLEGNDV
                     IGDSYQYMIERFASDAGKKGGEFFTPSMVSELLARLVKPKENDRIYDPTAGSGSLLIR
                     TVNQVPSRKVAVYGQERNGQTHSLAMMNMFLHEIDDAKIEWGDTLSNPLHLEDGKLMK
                     FQVIVANPPFSLDKWAMGFAG"
BASE COUNT          667 a          252 c          390 g          476 t
ORIGIN      
        1 agaaaagcaa gaagcggcta tggtagaagc cttcaatgat atttggaaga ttaaagaaga
       61 ctataatgtt agtatgagaa aagcagccta tctccactca gtaaaaaagg tggcagaggt
      121 tatgaaatta aggggctggt attagaatat aggattggca aaagagactt cccttcagga
      181 aatcctgagg ggaagtcttt tttagaaaaa tttacaaaat attccttgac attatattta
      241 aactgaacta gaatgaaagt aacacaaaaa taaaaattgc aatataacaa aaataacact
      301 aaggaggtgc ccaatgaagc taggcagtat atcagaaatt agaacaggta tgattttatc
      361 aaggaaaagg gcagatagtg aatttgaaaa ggcggaaacc tataaactta tagctataaa
      421 aaatgtagat gaagaaggac tttttaataa tgagcccttt gaagactttg atagtaatga
      481 caagttagac caggtttact ttacaaagga gggggatatt ttatttagac ttagccatcc
      541 atatacagct ctatatatag gaaagcttta tgaaaatcta ttaatcccat cacattttgc
      601 caaaatcagg atcaaagacc atcgttactt gcctgaatat gtggcttggt accttaatag
      661 agaaaagatt aaaagagagt tattaagata tcagactgga agtgtagtgc ctactacaaa
      721 tattagaatt ttaagttccc ttaggattaa agctatggat aaggctaagc aggaaagcat
      781 tattaattat aacaagcttt acttaaggga aaagagctta ctcaaaaggt taatgaagga
      841 aaatgaaaga ctaaataagg ctataactaa taaattgata gggggaaaag ataatgacta
      901 aaaagaccac tcaaactgaa ataaaccagg tcctatggga ggccagtgat acctttaggg
      961 ggaaaattga ttccagtatt tacaaggact atgtactggt tatgctgttt gtaaagtatt
     1021 tatctgatgt atatgctgaa agggtagaat atttccatga aagatatgaa ggaaaccagg
     1081 aaagaattga aagggccctg tctagggaaa gatttatttt aagtgaaaag acaagctttg
     1141 actacctata tagtaaaaga catgacccag aaattggaga aattatcaac aaggctttag
     1201 ataagataga gtcagataat gctggaaaac ttaggggagt atttagaaat attgacttta
     1261 atagtgaaac cgtcctaggg gagaccaggg atagaaatgc tatgcttagg accctattgg
     1321 aggactttaa taagctagac cttaggccat ctaaattaga aggtaatgat gtaataggag
     1381 attcttacca atatatgata gaaagatttg cttctgatgc aggtaagaag ggaggagaat
     1441 ttttcacgcc ctctatggta tcagaacttt tggccagact ggttaagcct aaggaaaatg
     1501 atagaatcta tgaccctaca gctggttcag gatccttgct tattagaact gtaaaccagg
     1561 ttcccagtag aaaagtggca gtatatgggc aggaaagaaa tgggcagacc cattccttag
     1621 ccatgatgaa catgtttctg catgaaatag atgatgcaaa gatagagtgg ggagataccc
     1681 tatctaatcc tcttcactta gaagatggaa agcttatgaa atttcaggta atagtggcaa
     1741 acccgccttt ctccctagac aaatgggcca tgggctttgc cggtg
//
LOCUS       DATGSI010000689         1479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_327351, whole genome shotgun sequence.
ACCESSION   DATGSI010000689 DATGSI010000000
VERSION     DATGSI010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327351"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..>1479
                     /locus_tag="VK031_10450"
     CDS             359..>1479
                     /locus_tag="VK031_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLS54386.1"
                     /translation="MKWYKYPVENLIEKFNSHADHGLSDFKVNAKLKKYGYNELKEET
                     GRSFYKKLVDQFSDFLVLILIAAAIVSIFVGEKTDSIVILAIVIINAFLGIYQEGRAE
                     KALESLKEMASPTAKVIRNGHTEIIAASHLVPGDIVLLDTGDIVPADLRLTKSWNLQI
                     EEASLTGESVPVEKDYSLIFDSDVSLGDRTNMAYMSTIVTYGRAEGIVVGTGHDTEIG
                     KIATAIQTYEDESTPLQNQLNQLGKFLGLITIAICGIVFIVGLMQNRGFLEMLMVSIS
                     LAVAAIPEGLPAIVTIVLALGMNKMVKRHAIVKKLLAVETLGSTTVICSDKTGTLTQN
                     EMTVVKLYANNKLIKVTGSGYEPEGEFLEGQTKVNLKET"
BASE COUNT          486 a          226 c          301 g          466 t
ORIGIN      
        1 tttccatgta atggcagtta ccactgcttc cgctggataa ggaacaaaat ttaatagtca
       61 aaaaaattcc cccactttct ctgattatta tattataatc tatttatgat gaaaacaaaa
      121 atatatatgt cttaaattta tgctaggact atctaatccg taacgccaga ttagaatcta
      181 taagactata agcctttgaa actacatata tatgcaatag gggccataaa aattattatt
      241 tttgagattt tgggaccttg gtttaattaa ttaaagccag gtctatttta tgctgatttt
      301 catttatata tagtcctttt taaggtatac tattatagta taaggaggat ggaattatat
      361 gaagtggtac aaatatccgg tagaaaactt aattgagaag tttaacagcc atgctgacca
      421 tggacttagt gattttaagg tcaatgccaa gttaaaaaaa tatggttata atgaattaaa
      481 ggaggaaaca ggcagaagtt tctacaaaaa attagtagat caattttctg attttctcgt
      541 tttaatatta attgcagcag caattgtatc tatttttgtt ggggaaaaga ctgactctat
      601 tgtaatcttg gccattgtaa tcataaacgc ctttttaggg atttatcagg agggacgggc
      661 cgagaaggcc ttagaatcct tgaaggagat ggcatctcct actgctaagg ttattcgaaa
      721 tggacatact gaaataatag ccgctagcca tctggtgcct ggtgatatag ttttacttga
      781 tacaggcgat attgttcctg cagatttaag attaacaaaa agctggaatc tgcaaattga
      841 agaagcctcc cttacaggcg aatcagtacc tgtggagaag gattatagtc ttatctttga
      901 ttctgatgta tccctaggtg ataggactaa tatggcatat atgagtacca tagttacata
      961 tggaagagct gaaggtatag tggtaggtac aggccatgat acagaaattg gaaagattgc
     1021 taccgccatt caaacctacg aggatgaatc tactcctttg cagaatcaac tgaatcaact
     1081 aggtaaattt ttaggcctga ttactatagc catatgcgga atagtgttca ttgttggtct
     1141 tatgcaaaat agaggatttt tggagatgtt aatggtctcc attagcttgg cagttgcagc
     1201 tattcctgaa ggcctacctg ctattgttac aattgttttg gccctgggga tgaataagat
     1261 ggttaaaaga catgccattg taaaaaaact attggctgtt gaaactctag gtagcaccac
     1321 tgttatttgt tccgataaaa ctggaaccct tactcaaaat gaaatgactg tggtcaagct
     1381 atatgccaat aataaactta ttaaggtgac aggaagcggt tatgagcctg aaggtgaatt
     1441 tctagaagga cagacaaagg ttaatttaaa agagaccag
//
LOCUS       DATGSI010000690         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_519350, whole genome shotgun sequence.
ACCESSION   DATGSI010000690 DATGSI010000000
VERSION     DATGSI010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519350"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..388)
                     /locus_tag="VK031_10455"
     CDS             complement(65..388)
                     /locus_tag="VK031_10455"
                     /inference="COORDINATES: protein motif:HMM:NF025688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HLS54387.1"
                     /translation="MKTKKLIDLAKTIRSKNASPDRVTFDIIFREKEDYELVKNSGAL
                     TKESVAELFDIDIARITDFVEFDPAYAIKFTISRAFPSGSPGEGDIFGCQQYPPLLEV
                     EIPVS"
     gene            complement(401..1771)
                     /locus_tag="VK031_10460"
     CDS             complement(401..1771)
                     /locus_tag="VK031_10460"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HLS54388.1"
                     /translation="MSKKELRIHCSNGHLGFAPTKEESFHIGARTKPDYYICDSGSND
                     IGPAPLGADTSPSLKVWQKHDLELMLLAAREQGVPMIVGSAGDTGSNSRVDMFVEMIK
                     EIAEEHDLEPFKLAYFYSEVDKEILREKMEKGIKIEGLDNRPDLTLEELDGTTRIVAV
                     AGVHPYMKALDMGADVIIGGRSSDIALFAAPALREGFPENLSYYLGKVLECASFCAEP
                     YGAKETVIGTITHEDVKVEAMAPWQRCTVASVAAHAMYERSNPYYEYFAGGMLDMSEC
                     VYEQYDEKTTRITNPKYVPVDGKVKVKLEGSSKVGEMYIGIAGVRDPYTIKNIDKVLE
                     LAREHVEERFGKEGYELHYRVYGKDGVMGDLEPVKEIKSHELCIVVQGVAPTKEMAQE
                     VAMIGTRQIFYARLPDVKGTAGTAAFVVDEVLPATPAYRWSLNHIVEVDDPMEFFDVK
                     LIEIGK"
     gene            complement(2059..>2805)
                     /locus_tag="VK031_10465"
     CDS             complement(2059..>2805)
                     /locus_tag="VK031_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLS54389.1"
                     /translation="IPENLIESELFGHEKGAFTGAIQTKKGKFELAHKGTLFLDEIGE
                     LPLPMQVKFLRVLQEKQIYRIGSEQAIDVDVRIIAASNRDLKEEVEKGNFREDLYYRL
                     NVVNIEIPPLRERKEDIPLLAKKFLDDFSKEYGKDLEYIDVEAINYLVEHKWKGNVRE
                     LQNVIERAVLFADADKEILLPEDLPAELVGKRDEEIEERADMTLKDYEKILIENTLKR
                     NSGNKSKTAKILGIKRQTLYNKIKEYGIEA"
BASE COUNT          794 a          645 c          366 g         1000 t
ORIGIN      
        1 ttcctcccat aattaatggg ccgcggccct aaataaccgc ggcatttata atccattttt
       61 aaacttagct aactggtatt tctacctcta ataaaggtgg atattgctgg caaccgaata
      121 tatctccctc tccaggacta ccactgggga aagccctaga gatggtaaat ttaatggcat
      181 aggctggatc aaactccaca aaatctgtta tcctagctat atctatgtca aatagttctg
      241 ctacactttc cttagtgaga gcaccactat tttttactag ttcataatct tctttttctc
      301 taaagataat atcaaatgta actctatcag gactagcatt tttacttctt atggttttag
      361 ctaaatctat aagcttttta gtcttcatat ttacactccc ctattttcca atttcaatta
      421 atttaacatc gaagaactcc attggatcat caacttcaac aatatgattt aatgaccatc
      481 tatatgctgg agttgcaggt agtacttcat ctactacaaa agctgctgtt cctgcagtac
      541 ctttaacatc tggtagtcta gcatagaata tttgtcttgt acctatcata gctacctctt
      601 gtgccatttc cttagttggt gctacacctt gtactactat acataattca tgagatttaa
      661 tttctttcac tggttctaaa tctcccataa caccatcttt accataaact ctgtagtgaa
      721 gttcatagcc ttcttttcca aatctttctt caacatgttc tctagctagc tccaatacct
      781 tgtcaatatt cttgatagta taggggtctc tcactcctgc aatacctata tacatttctc
      841 caactttaga cgaaccctct aattttacct ttactttccc atctactggc acatactttg
      901 gattagttat acgagtagtt ttctcatcat actgctcata aacacattca gacatatcca
      961 gcattccacc agcaaaatac tcataatatg gattagatct ttcatacatg gcatgggctg
     1021 ctactgaagc aactgtacat ctttgccatg gagccatagc ctcaactttt acatcctcat
     1081 gagttatagt tccaattact gtttcctttg ctccataagg ctcagcacag aaagaagcac
     1141 actcaagtac ctttcctaga taataagata gattctctgg gaatccttct cttaaggctg
     1201 gtgcagcaaa tagggctata tcagatgatc ttcctccaat aattacatct gcccccatat
     1261 ctaaggcctt catataggga tgtacacctg ccactgcaac tattcttgta gttccatcta
     1321 actcttcaag ggttaagtct ggcctattgt ctagtccctc tattttaatt cctttttcca
     1381 tcttttctct taatatttct ttatctactt ctgagtaaaa gtaggccagt ttaaatggct
     1441 ccaggtcatg ttcttctgca atttctttaa tcatttcaac aaacatatca accctgctat
     1501 tggatccagt atctccagca gaacctacta tcataggaac tccctgctcc ctagctgcca
     1561 gtaacattaa ctctaaatca tgcttttgcc ataccttcaa acttggtgag gtatctgcac
     1621 ctaaaggtgc tggaccaata tcattgctcc ctgagtcaca tatataatag tcaggcttag
     1681 tcctggctcc aatgtggaaa ctttcctcct tggttggcgc aaatcctaaa tgaccattgg
     1741 aacaatgaat ccttagctct tttttactca ttttaactcc cccttaatta ttaggtcttt
     1801 ttccacctat aattattgca aataatatgc caacctctat atatttaaaa gtttccagcc
     1861 tataaacagt caaatattaa acctttatta aatttgccta aaataagggg gtaaataggc
     1921 aaaaaaataa ggacccatcc atttaagtta aatactaagc cttttaaagg taacttaaat
     1981 agaaacatcc caaaaagtgt ataaaaatta gactataaaa agcaagggct cctgtctact
     2041 tgttagatat ttttacattt aggcctctat cccatactct ttaatcttgt tataaagggt
     2101 ttgtcttttt atacctaata ttttggctgt cttagacttg tttccactat ttcttttcag
     2161 tgtattttct attaatatct tctcataatc ttttagggtc atgtctgccc tttcttctat
     2221 ttcttcatcc ctttttccaa ctagctctgc tggcaggtct tctggtaaaa gaatttcctt
     2281 atcagcatct gcaaataata ctgccctttc tattacattt tgtaactccc ttacatttcc
     2341 cttccactta tgttccacta agtaattaat tgcctcaaca tcaatatact ctaaatcttt
     2401 cccatattct ttagaaaagt catccaaaaa cttcttagcc agcaagggta tatcctcctt
     2461 tctctctctt aaaggaggaa tttcaatatt aaccacattt aacctataat ataagtcctc
     2521 tctaaagtta cccttttcta cttcctcctt taaatcccta ttagatgcag caataatcct
     2581 tacgtccaca tctatagcct gctctgatcc tatcctatat atttgttttt cctgtaatac
     2641 tctaaggaat tttacctgca ttggaagtgg taattcgcct atttcatcaa ggaaaagagt
     2701 tcctttatgt gccaattcaa atttaccctt tttagtttga atagcacctg taaaagcacc
     2761 tttttcatgg ccgaatagtt ctgattcaat taagttttca ggtat
//
LOCUS       DATGSI010000691         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_327437, whole genome shotgun sequence.
ACCESSION   DATGSI010000691 DATGSI010000000
VERSION     DATGSI010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327437"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VK031_10470"
     CDS             complement(<1..295)
                     /locus_tag="VK031_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HLS54390.1"
                     /translation="MDYFLIAAPVVGILALAFAYYKASSIDKVDPGKERMQEISSHIE
                     RGAMAFLSRQYRSLGIFIAVLFIVLTFAPNLGLSTAICFLLGALFSIAAGYIGM"
     gene            complement(312..539)
                     /gene="secG"
                     /locus_tag="VK031_10475"
     CDS             complement(312..539)
                     /gene="secG"
                     /locus_tag="VK031_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HLS54391.1"
                     /translation="MQTFFSIVLLISALGVIISVLLQEGKEGGLSALGGNAQEALWGV
                     NRGKSKEVILQRITIVSATIFMVATVILAAR"
     gene            complement(738..>1548)
                     /gene="eno"
                     /locus_tag="VK031_10480"
     CDS             complement(738..>1548)
                     /gene="eno"
                     /locus_tag="VK031_10480"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HLS54392.1"
                     /translation="IQEFMIMPVGAKNFREALRMGAEIFHSLSEELKSRGLNTSVGDE
                     GGFAPNLSSNEEALATIVTAIEKAGYKPGEEVVLALDVAATEIYDKERKVYKLEGEGR
                     ELNVEEMIDFYEELVEKYPVISIEDGLDEDDWEGWKALTERLGKKIQLVGDDLFVTNT
                     ERLKKGIDQGITNSILIKLNQIGTITETLDAIELAKVNGYTAVISHRSGETEDTTIAD
                     LAVALNTGQIKTGAPSRTDRVAKYNQLLRIEDELWNAAQYKGMDIFYNLDK"
BASE COUNT          437 a          348 c          210 g          553 t
ORIGIN      
        1 tcatgccaat atagcctgct gcaattgaaa atagtgcacc taataaaaag caaatggctg
       61 tgctaagtcc taaatttggt gcaaatgtta aaacaataaa taaaactgca ataaagattc
      121 ctaatgacct gtattgtctt gataagaaag ccatggcccc tctttcaata tggctagata
      181 tttcttgcat cctttctttg cctggatcta ccttatcaat actagacgct ttgtagtatg
      241 caaaagccag tgccaatata cctactactg gcgctgctat taaaaaataa tccattaacc
      301 ttcctcctct gttatcttgc tgctaagatc actgttgcca ccataaatat tgttgctgaa
      361 acaatagtta ttctttgcaa tattacttct ttacttttgc ctctatttac gccccataaa
      421 gcctcttgag catttcctcc tagggcactt aaaccgcctt ctttaccttc ctgtaaaaga
      481 acacttataa ttacccctaa ggcagatatc aataatacta tggagaaaaa tgtctgcatc
      541 cttgcacctc ctattttaga gtcttgttga aaacttattc atctcattaa cattaatatt
      601 ttaacatagg ccatatataa aatcaatcaa aactacctat ctaggctacc ctttcaaacc
      661 aaggcttgtt ggcctatctc ccttaaaatt acttaaggac cctaactaga ttatagggtc
      721 cttaagtaaa aattatacta tttgtctaaa ttgtagaata tatccatacc cttgtattgg
      781 gctgcattcc ataattcatc ttctattctt aaaagttgat tgtatttggc taccctgtct
      841 gttctagaag gtgcaccagt cttaatttga ccagtattta aagctaccgc cagatcagct
      901 attgttgtat cttctgtctc accagaccta tgggaaatta cagctgtata gccatttacc
      961 tttgccaact caatagcatc taatgtttca gtaatagtac ctatttggtt taattttatt
     1021 aatatagagt ttgttatacc ttggtcaata ccctttttca atctttcagt attggttaca
     1081 aataggtcat ctcctaccag ctgaatcttt ttacctaatc tttcagttaa agccttccag
     1141 ccttcccaat cgtcctcatc taatccgtct tcaatagaga ttactgggta tttttcaact
     1201 agttcttcgt agaagtctat catttcttcc acattaagtt ctctaccttc accctctagt
     1261 ttataaacct ttctttcctt atcatagatt tcagtggctg ccacatccaa agctaatact
     1321 acttcttctc ctggcttata acctgccttt tctatggctg ttactatggt tgctaaggct
     1381 tcttcgttgc tggataggtt tggtgcaaaa ccaccttcat cacctacaga agtatttaaa
     1441 cctcttgatt ttaattcttc acttaagctg tggaagattt ctgctcccat tcttagagct
     1501 tctctaaagt ttttagctcc tacaggcata atcataaatt cttgtata
//
LOCUS       DATGSI010000692         1132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_424102, whole genome shotgun sequence.
ACCESSION   DATGSI010000692 DATGSI010000000
VERSION     DATGSI010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424102"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..>1132)
                     /locus_tag="VK031_10485"
     CDS             complement(71..>1132)
                     /locus_tag="VK031_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54393.1"
                     /translation="IANRTRFQIRAWNFANTGYSNESTPRVVTVEGESNEFEGSIYGD
                     KVCTGIVTGGDVIYGRLHRVELSEASDNGVYQLGTTSKLSIGTVVYRGSEEGLVIGGN
                     GCEIDSYKIVGRSSAAISMQSGALKITDMRIEGNPEIGSPASILSLRPGYTAKKIEIG
                     NITGTIWGNTLMNMSPGACRSFRLLSADLEFLYTDDRAGAITQWWNLDNAEEIFIQNA
                     KVVIRDIEGDLAGSGSIFEVRLGSQGGAGNKVTLINPEIYIRNNDGGSVPGNSVRIVR
                     LNRGNFSIQGVKIFTNLSHMVGREANYLGPFVAGVNISNNKPTGTFPIGTTVYRGDGA
                     SSPYGWVYTQDGWEDLTIP"
BASE COUNT          318 a          285 c          187 g          342 t
ORIGIN      
        1 gccggtacag gatcttccag ctttgccagt gcggcttcgg cttcttgaat gatattttct
       61 aaatgctttc tcatggtata gtaagatcct cccaaccgtc ttgtgtatat acccatccat
      121 aaggagacga agctccatca cctctataaa ctgtagtccc tattggaaag gttccagttg
      181 gtttattatt agatatatta acccctgcta caaaagggcc aaggtaattc gcctccctac
      241 caaccatgtg agataagtta gtaaagattt taactccttg gatagagaag ttacccctat
      301 ttagacgaac tattctaaca ctgttccctg gaacagaacc tccatcgtta ttacgaatat
      361 agatctcagg gtttattaaa gtgactttat tcccagcacc accttgtgag ccaagacgta
      421 cctcaaatat acttcctgaa ccagcaagat ctccttctat atctcgtata actaccttag
      481 cattctgaat aaagatctct tctgcattat caagattcca ccattgagtt atggctcctg
      541 ctctatcatc agtataaaga aactctaaat ccgcagataa aagcctaaaa cttcggcagg
      601 ctccaggaga catgttcatt aatgtattac cccatatagt tcctgtaata tttcctattt
      661 ctattttctt tgcggtatag ccggggcgaa gactcagaat gctggcaggg ctacctattt
      721 ccggatttcc ctctatcctc atatcagtaa tcttcagcgc cccagattgc attgatatag
      781 ctgctgaact ccgccctacg atcttatagc tatcaatctc gcaaccgttc ccaccgataa
      841 ccagtccttc ttcgcttcct cgatagacaa cagttccaat tgatagttta gaggttgtac
      901 ctaactgata aacaccattg tccgaagcct cagataattc tactcgatga agccttccat
      961 atataacatc tccgccagta acgattccag tacaaacttt atcaccataa atagatcctt
     1021 caaactcatt agattcccct tcaactgtaa cgactcgagg cgtggactcg ttagagtaac
     1081 ctgtattagc aaaattccag gctctaatct ggaaacgtgt tcggtttgct at
//
LOCUS       DATGSI010000693         4835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_278585, whole genome shotgun sequence.
ACCESSION   DATGSI010000693 DATGSI010000000
VERSION     DATGSI010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278585"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..2311)
                     /locus_tag="VK031_10490"
     CDS             complement(212..2311)
                     /locus_tag="VK031_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HLS54394.1"
                     /translation="MDILQVLIEEFQLKEKHVKNVIKLLDEGNTIPFIARYRKELTGG
                     MEDTVIRDLSERLGYLRNLENRKEEVIRLIEEQGKLNDELKKEIQKAISLQRVEDLYR
                     PFRPKRRTRATVAKEKGLEGLAELILKQELDAKDLQEELVKYIDKEKEINNEEEALQG
                     AMDIIAEIVSDHADFRRDIKEIILSQGLIISQGTDTDEDSVYEMYYDYKEPIKSIANH
                     RILAINRGEKEKKLRVNLYIGDEKTINLIKDRMITNPKSACLDYLSQAIEDGYKRLLY
                     PSIEREIRNDLKERAEEEAINVFALNLEPLLMQAPVRGKFVMGIDPAYRTGCKVAVLD
                     DTGRLLDYTVVYPTEPQKKVDKTKHTLTSLINKYGVEIIAIGNGTASRETEKVVAELL
                     RELEKEVYYIIVNEAGASVYSASAVGVKEFPDLDVTIRGAISIGRRLQDPLAELVKIE
                     PRHIGVGQYQHDLNQKRLNEALEAVVEDCVNRVGVDLNTASPSLLAYVAGISARVAGS
                     IVDYRERYGKFSSRKQLLEVKGLGPKTFEQCAGFLRITDGDNPLDNTAVHPESYDIAE
                     KLLEMEYKNMPLEKLAERLDVGKPTLKDIIEELEKPGRDPRDEMPGPILKQDVLSMED
                     LRVDMVLNGVVRNVVDFGAFIDIGVENDGLVHISQLSEKYIKHPKEVVTVGDIVEVKI
                     IDIDKSRGRISLSMKDV"
     gene            complement(2410..3517)
                     /gene="prfB"
                     /locus_tag="VK031_10495"
     CDS             complement(join(2410..3441,3443..3517))
                     /gene="prfB"
                     /locus_tag="VK031_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128425316.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HLS54395.1"
                     /translation="MDTVNIVKERICEIKEMVKELGVSLDIEKLKEKLKKREELTYQA
                     DFWDNTERAQRIMQEIRKLNREIKSYDSLLSEIEDLEIFIQLVIEEESEENYGEIKEA
                     LKEIEKKAEEFRISALLRGEFDKNNAILSINSGAGGLEAQDWAEMLLRMYRRWAEGKG
                     YQVETLDLQPDTEAGIKSVTLLIKGFNAYGYLKAEKGVHRLVRISPYDSSGKRHTSFA
                     SVDIFPELDDELDVEINERDLKIDTYRSSGAGGQHVNTTDSAIRITHLPTGIVVQCQS
                     ERSQHSNRATAMKMLKSKLMQLKEEEQKEKIEDLQGNYSQISWGSQIRSYVFHPYSMV
                     KDHRTNAEVGEVERVIDGDIDLFINEYLKQRALP"
     gene            complement(3559..>4835)
                     /locus_tag="VK031_10500"
     CDS             complement(3559..>4835)
                     /locus_tag="VK031_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HLS54396.1"
                     /translation="REAEIVAQAGRYGAVTIATNMAGRGTDIVLGGNPEYLAKLEMKK
                     KGYPDEILEEVDGFYDTDQEEVLEARKVYDQLVKKYKEETDKEHKKVIEAGGLHIIGT
                     ERHESRRIDNQLRGRAGRQGDPGSSRFYISLEDDLMRLFGGDRLRNIVETMNMPEDEP
                     LEHAILTRSIESAQKKVEGNNFAIRKHVLKYDDVMNKQREIIYGERRKVLEGEDLKEH
                     IEGMITSLIESGIELYTRQSPYPEEWDIEGLENYLGKIFDFQPGFLLKRDIKSMDKKE
                     LQEEILNMAQDKYKEKEEEFKEERFREVERVILLQVVDRKWMDHIDAMDQLRQGIGLR
                     AYGQEDPVRAYQIEGFDMFEEMTHSIWEDTVRYLYHVENPEKIERKRVAKEIEPRETR
                     ASGTVVKGRKIGRNEPCPCGSGKKYKRCCGRN"
BASE COUNT         1185 a         1191 c          627 g         1832 t
ORIGIN      
        1 acctggagtc accagtcttc cttctgcatc tattaccttg gcatctaaag gcgctataat
       61 atcctttcca atatctacta tcttaccatc ctttaccaag attgacccat tttcaattac
      121 ttcatccttc attgtgtaaa taagtccatt ttttataaat atcatttata tcccccttta
      181 tttaaattat attttatttt ctttttataa attatacatc cttcatgctt aaagatatcc
      241 tgccccttga tttatcaata tctattattt tcacctcaac tatatcccct actgtaacta
      301 cttccttcgg atgctttata tacttttcag aaagctgtga tatatgaacc aggccatcat
      361 tttcaacccc tatatctata aaggcaccaa aatcaactac atttcttact accccattta
      421 aaaccatatc tacccttaaa tcttccattg ataaaacatc ctgctttaag attgggcccg
      481 gcatttcatc ccttgggtct ctacctggct tttccaactc ctctattata tcctttaagg
      541 taggcttgcc tacatctagc ctttcagcta gtttttccaa tggcatattt ttgtactcca
      601 tttctagaag cttctccgct atatcgtagg attctgggtg aactgccgtg ttgtctaaag
      661 gattgtctcc atctgttatt cttaaaaaac ctgcacactg ctcaaaggtt ttaggtccta
      721 gccccttcac ttccagaagt tgttttcttg atgaaaattt accatatctt tccctataat
      781 caactatact accagccacc ctggcagaaa ttccagccac ataggcaaga agggaaggac
      841 tggctgtgtt aagatctacc ccaaccctgt ttacacaatc ctctactaca gcttctaggg
      901 cctcatttaa ccttttctga tttaagtcgt gctggtactg gcctacacct atatgtctag
      961 gttcaatctt taccagttca gccaaaggat cctgcaatct cctgccaatg gagatggccc
     1021 ctctaatagt tacgtccaag tctggaaact ccttaacgcc aactgctgag gcagagtaaa
     1081 ccgaggctcc agcttcattt actattatgt agtagacttc cttttcaagc tctcttaaca
     1141 attctgcaac taccttttct gtttctctag atgcagttcc atttcctatg gcgattattt
     1201 ctaccccata tttatttatc aatgaggtta gggtatgctt ggtcttatct accttctttt
     1261 gaggttctgt tggataaact acagtataat ctaataatct tcctgtatcg tctaatacag
     1321 ccaccttgca acctgtccta taggccggat ctattcccat cacaaatttg cctcttacag
     1381 gggcctgcat taataagggt tctaaattta aggcaaatac attaatggct tcctcttcag
     1441 ccctttcttt caaatcattt cttatctccc tttcaatgga agggtataat agccttttat
     1501 atccgtcctc tatggcctgg cttaaataat ctaggcaggc tgatttaggg tttgtaatca
     1561 tcctatcttt aattaggttg attgtttttt catctccaat gtataaattt actcttaatt
     1621 tcttttcctt ctctccccta tttatggcca atatcctatg gttggcaatt gacttaatag
     1681 gctccttata atcataatac atttcatata ctgaatcttc atcagtatca gttccctggc
     1741 ttataataag tccttgggat aggataattt cttttatatc tcttctaaag tctgcatggt
     1801 cggaaactat ttctgcaatt atatccatag ccccttgtaa ggcttcttcc tcattattta
     1861 tttccttttc cttatctata tatttaacaa gctcctcttg aaggtccttt gcatctaatt
     1921 cctgctttag tataagctca gccaagcctt caagaccctt ttccttggcc acagttgccc
     1981 tagttctcct tttgggccta aagggcctat ataaatcttc aactctttga aggcttatgg
     2041 ctttttgaat ctcctttttt aattcgtcat ttaattttcc ttgttcctct attaggcgaa
     2101 ttacttcttc cttcctgttt tccaggtttc tcaagtagcc cagcctttca gacaaatctc
     2161 ttattacagt atcctccata ccgccagtta attccttccg atacctggct ataaagggga
     2221 tagtatttcc ttcatctaaa agttttatta catttttaac atgcttttct tttaattgaa
     2281 attcctctat caatacctgt aatatatcca tcttattttc ctttccttcc atatggtttc
     2341 ttctttattc tagattggaa ctgcttattc cttctaagaa tcaaatcttt aaaaagttta
     2401 agcccttacc taaggaaggg ccctttgctt taagtattca tttatgaaca gatctatatc
     2461 tccatctatt accctttcca cttcccctac ttcagcattg gtcctatgat ctttcaccat
     2521 actataggga tggaatacat aggatcttat ttgtgatccc caagatattt gtgagtaatt
     2581 tccttgcaga tcctctattt tttctttttg ttcctcttct tttaattgca tcagctttga
     2641 ctttaacatt ttcattgccg tagccctatt gctatgttgg gacctttcag actggcattg
     2701 taccactata cctgtaggca ggtgggttat tcttatggca gaatcagtag tattaacatg
     2761 ctgccctcca gctccagagg acctataagt gtcaattttt agatcccttt catttatttc
     2821 cacatcaagt tcatcatcta gttctggaaa tatatctaca ctggcaaagg aggtatgtct
     2881 tttacctgag gaatcatagg gagatattct aaccaatcta tggactccct tttcagcctt
     2941 taagtagcca taagcgttaa aacctttgat taaaagggtt acagacttaa tcccagcttc
     3001 agtatccggc tgcaagtcaa gggtctccac ctgatagccc tttccttctg cccatcttct
     3061 gtacatcctt agaagcattt cagcccaatc ttgagcttcc aagcctccag cacctgagtt
     3121 gattgataaa atagcgttgt ttttatcaaa ttctccccta agcaaggcag agatcctaaa
     3181 ctcctccgcc ttcttttcta tttcctttaa agcttcttta atttctccat aattttcttc
     3241 agactcttct tctataacca gttgaataaa tatttccaaa tcctctatct cagataataa
     3301 gctatcatat gatttaatct ctctgtttaa tttcctaatc tcttgcatga ttctttgggc
     3361 tctttctgta ttgtcccaaa aatcagcctg ataagttagt tcttcccttt tttttaactt
     3421 ttcttttagc ttttctatgt caaagagaca cacctaattc ttttaccatt tcttttattt
     3481 cacatattct ttcctttact atatttacag tatccatttt ttacaccttt ccatatgctt
     3541 tcattttgga ttatataatt agtttctccc acaacatctc ttatacttct taccactacc
     3601 acagggacag ggttcattcc ttccaatttt tctcccctta accactgttc cactagctct
     3661 tgtttcccta ggctctattt cctttgccac tctttttctt tctatttttt ccgggttctc
     3721 cacatggtat aaatacctta cagtatcttc ccaaatagag tgggtcatct cctcaaacat
     3781 gtcaaatcct tctatttgat aggcccttac cggatcctct tgcccatagg ccctcaaccc
     3841 aattccctgc cttaattgat ccatagcatc tatatgatcc atccattttc tatctactac
     3901 ttgcagtaga attacccttt ctacttccct aaatctttct tctttaaatt cttcctcttt
     3961 ctccttgtat ttatcctggg ccatatttag aatttcctct tgtagttcct ttttatccat
     4021 agacttaatg tctcgtttta gtaaaaagcc aggctggaaa tcgaaaattt tgcctaaata
     4081 gttttccaat ccttctatat cccactcttc tggatatggg gattgtctag tgtataattc
     4141 tataccactt tctattaggc tggtaatcat accctctatg tgttccttta aatcttcccc
     4201 ctctaagacc tttcttcttt ctccatagat tatttccctt tgcttattca ttacatcatc
     4261 atatttcaat acatgcttcc ttatggcaaa gttgttgccc tccacctttt tctgagcaga
     4321 ctcaatgctt cttgtaagga ttgcatgctc cagtggctca tcttcaggca tattcatagt
     4381 ctctacaata tttcttaacc tatctccacc aaaaagcctc attaaatcgt cttctaggga
     4441 aatataaaat ctggaactgc ctggatcccc ctgtctacct gctcgacctc ttaactgatt
     4501 gtctatacgt ctggactcat gtctttcagt acctattatg tgaagaccgc ctgcttctat
     4561 gaccttttta tgttctttat cagtttcctc tttatatttt ttgaccaact gatcataaac
     4621 ttttctagcc tctaaaacct cttcttggtc tgtatcataa aagccatcta cctcttccaa
     4681 aatctcatct ggatagccct tctttttcat ttctaattta gccaaatatt caggattgcc
     4741 ccctaataca atatctgtac ctctacctgc catattggta gctatggtta cagcaccata
     4801 tctacctgcc tgggcaacta tttcagcctc ccttt
//
LOCUS       DATGSI010000694         1832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_93815, whole genome shotgun sequence.
ACCESSION   DATGSI010000694 DATGSI010000000
VERSION     DATGSI010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93815"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1552
                     /locus_tag="VK031_10505"
     CDS             239..1552
                     /locus_tag="VK031_10505"
                     /inference="COORDINATES: protein motif:HMM:NF015563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HLS54397.1"
                     /translation="MGKNNKGNAKGLLPLALFLLMFLGTAAITGDFYKMPVLVAFFIV
                     SGVALAMNKNRSLTENVELFSKGAGHVDIMIMVIIFLLAGAFANVAREMGGVESVVNF
                     GLSIIPTNLLIPGVFLICCFVSLSMGTSMGTIVAIAPIAKGIADKTAITYPIALGAVV
                     SGAMFGDNLSMISDTTIAAVRTQGVDMKDKFKFNFLLVLPAAIATAIIYGIITSGTTL
                     VLDETFEYSFVKILPYLTVLIGALIGFNVMLVLAVGVVFAGVLGIVGGSFDIFGLLQA
                     IQEGMEGMMDLAMIAIVVGGLVELIKDAGGIDWLLNTINSRIKTKKGAEFGIAALTSV
                     ADIATANNTIAIVMAGPLAKDIADEYDIDPRRTASLLDIWASVWQGLIPWGGQLLAAA
                     GLAGISPFVIVPFIFYPMGMAVMAILGTVTGFPRFATKEIIEKAE"
BASE COUNT          567 a          259 c          403 g          603 t
ORIGIN      
        1 ttattcagga tatggatatg ccaatttcac taactgaata caaaatacct acagatcagt
       61 ttgaattaat ggctgaaaaa tgtaccaagg gcgggtatgt aggaagcttt aagaaacttt
      121 ataaggaaga tgtaatcaat attttaaatc tggctaggta gcttaataat acttagcctg
      181 tacagctgtt actgtagtaa gaaaataggt ttagtgaaaa ataatgaggg ggattagaat
      241 gggtaaaaat aataagggaa atgcaaaagg acttttacca ttggccttgt tcttgttaat
      301 gtttttaggt acagctgcta ttacaggaga tttttataag atgccagtat tggtagcctt
      361 ctttatagta tctggcgtgg ctttggccat gaataaaaac agaagtttaa ctgaaaatgt
      421 agaattattc agcaagggtg caggccatgt agacattatg ataatggtaa taattttctt
      481 attggctgga gcctttgcca atgtagctag agaaatgggt ggggtagaat ccgtagttaa
      541 ttttggtttg tctattatac ctaccaattt attaattcca ggagtatttt tgatctgctg
      601 ctttgtttca ttgtccatgg gaacatccat gggaaccata gttgctattg ctcctatagc
      661 caaaggtatt gccgataaaa ctgctattac ttatccaatt gctttaggag ctgtagtaag
      721 cggggccatg tttggagaca atttatctat gatttccgat actacaattg cagctgttag
      781 aactcaaggt gtagatatga aggataaatt caagtttaat ttccttctgg tattgcctgc
      841 agctattgct actgcaatta tatatgggat tataactagt ggaacaactc tggtactaga
      901 tgagaccttt gaatatagct ttgttaaaat actaccttac ctaactgtat taataggagc
      961 cttaatagga tttaatgtaa tgttggtatt ggcagttgga gttgtatttg caggtgttct
     1021 aggtattgta ggtgggagtt ttgatatctt tggactattg caagctattc aggaaggtat
     1081 ggaaggcatg atggatctgg ctatgatagc catagtggta ggtggattag tagaactgat
     1141 taaagatgca ggtgggattg attggctact taatactata aattctagaa ttaaaactaa
     1201 aaagggtgca gagtttggaa tcgctgcctt aacatcagtg gcagacatag ctacagctaa
     1261 taatactata gctatagtaa tggctgggcc attagcaaag gatatagctg atgaatatga
     1321 tatagatcct aggagaactg cctccctgtt agatatttgg gccagtgtat ggcaaggttt
     1381 gataccatgg ggtggccaac tattagcagc tgctggattg gcaggaattt caccctttgt
     1441 aattgtacct ttcatcttct acccaatggg tatggcagtt atggccatcc taggaactgt
     1501 tactggtttt ccaaggtttg caaccaaaga aataatagaa aaagctgaat aaaataagtt
     1561 taagctaagg tgtttcgtgg ctctgaccca gctaggaaac acctttttta taattcatca
     1621 actatatcct gcaggttagc ataaattata acactgatat actttgagaa atttgtgtca
     1681 taccatatat aatgttaatt taaattggaa gtttagtcaa gttgaggggg gtgggttatt
     1741 ttaatctaat acatatttta aatatagttt tcggtctttt atgcctcaat ttcaaggaag
     1801 acaaaatacc agattttatt taaaggaagc ta
//
LOCUS       DATGSI010000695         1336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_377230, whole genome shotgun sequence.
ACCESSION   DATGSI010000695 DATGSI010000000
VERSION     DATGSI010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377230"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VK031_10510"
     CDS             complement(<1..522)
                     /locus_tag="VK031_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLS54398.1"
                     /translation="MIKTKGIHHITSLVGNVEENVDFYGRILGLRLVKKTVNFDDPRT
                     YHLYFGDRKGNPGTVITFFPYEDTRAGIVGDGQVGITSYMIPNGASNFWKERLEKYNI
                     NYQLTSRLGEDFISFKDHHGLKIELVERDEANENQWSISGIGPDVAIKGFAGAVLYSI
                     KPYETAATLETLFG"
     gene            complement(574..>1336)
                     /locus_tag="VK031_10515"
     CDS             complement(574..>1336)
                     /locus_tag="VK031_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HLS54399.1"
                     /translation="IMSYNGDFQTYSKEAIKQAEKIANSKDKLISFIKDLPIESNIKW
                     DLFVVLEEAREHMKKYVSLMKKILPIFNEIYRAYKEKVNEYGIYLTGFLNKYGAKGLN
                     DISYSLIDPKLMDSEENNILISITGAYSIRIMGSTEKNYIAWGLKIEEAFKQMKEINE
                     NKTNERVQIFKNLGDKTRYEVVKLIAAGESSTKVIAEALNVSSATISYHLNNLVTSKI
                     IKLDKYNGKYGYIIDYKLLEDVIWGFKEDLNFPRV"
BASE COUNT          387 a          276 c          172 g          501 t
ORIGIN      
        1 tccaaacaag gtttctaaag tagctgcagt ttcataaggc ttaatagagt aaagaactgc
       61 tcctgcaaac ccctttatgg ccacatctgg gcctatgcca gaaattgacc attgattctc
      121 attagcttca tccctttcta ctaactctat ttttaatcca tggtgatcct taaaagatat
      181 aaagtcttca cctagccggg atgtaagctg gtagtttata ttgtattttt ccaacctctc
      241 cttccagaag ttactggctc catttggtat catatagctg gtaataccta cttgcccatc
      301 acctacgata ccagccctgg tatcttcata ggggaaaaat gtaatcactg tgccaggatt
      361 tcccttccta tcaccaaagt ataaatgata ggtcctaggg tcatcaaagt ttacagtttt
      421 tttaaccagc ctaagcccta gtatcctacc ataaaaatca acattttctt ctacattacc
      481 tacaagagat gttatatggt gaatgccttt agtttttatc ataaacctcc ccttcctata
      541 atagaattta atctatattt gccctcttat ctattatacc cggggaaagt ttaaatcctc
      601 tttaaatccc caaatgacat cttccaatag tttatagtct attatatatc catatttacc
      661 attatatttg tctagtttta ttatttttga tgtaactaga ttgtttaaat gataggaaat
      721 ggtagcacta gacacattta aggcctcagc tattacctta gtagatgatt cgccagctgc
      781 tataagttta accacctcat atctggtttt gtctcccaga tttttaaata tttgtacccg
      841 ctcatttgtt ttattttcgt taatttcctt catttgttta aaggcttctt ctattttaag
      901 accccaggct atataatttt tctctgtaga gcccataatt cttattgaat aggcacctgt
      961 tatagaaatt agtatattat tctcctctga atccataagc ttagggtcaa ttaaggaata
     1021 gcttatatca ttcagcccct tagccccata tttattcaag aaaccagtta agtatattcc
     1081 atactcattt actttctctt tataagccct gtatatttca ttaaaaatag gtaatatttt
     1141 tttcattaaa cttacatact ttttcatatg ctctcttgct tcttccaaaa ctacaaataa
     1201 gtcccacttt atattggatt caataggtaa atctttgata aatgaaatca atttatcctt
     1261 actattggct atcttttctg cttgttttat agcctccttt gaataagtct gaaaatctcc
     1321 attatatgac ataatt
//
LOCUS       DATGSI010000696         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_45029, whole genome shotgun sequence.
ACCESSION   DATGSI010000696 DATGSI010000000
VERSION     DATGSI010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45029"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VK031_10520"
     CDS             <1..660
                     /locus_tag="VK031_10520"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLS54400.1"
                     /translation="VIARRFERLEKLAERAADFEGEIYPLAGDMSKDEDIKNTVEKTV
                     EKYGRVDVVVNNAGVLDKFLSADNMEDEVWDFVMTVNVTGPMKLIREAMPYMKEQKSG
                     NIINISSVGGLFGAKGGLAYVTSKHAIIGMTKHIAAIFGDQGIRCNAIAPGTVETEIG
                     GTVKEPNMMVLDKVMKTAEVYPVVGEPDDIGNLILFLASDESKFINGATIVSDGGWTI
                     H"
     gene            complement(944..2746)
                     /locus_tag="VK031_10525"
     CDS             complement(944..2746)
                     /locus_tag="VK031_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HLS54401.1"
                     /translation="MKGTIVSAWVQTCKELYGEEITGEALSHFGMAPDRIFTPTEDID
                     DNIALGILEYIGDKLGKSSDEIWRNMGNHNIKTYTKVYPAFFRYKNLYSFLQAMYDIH
                     VIVTKRVPGAQPPILGIEPVDSHTAHMTYSSSRGMFAYFLGMLEGAAKHFEEDIQVDT
                     IEQTDTFLKLAITFPEEIYYEKNFIFNKLMSLGLIKSMEVKIALANLLFVGVPSILIF
                     NYLPKKIALPSILGLSFLIPALVSRGLFLPLNHIKAHLDEIKNKNFSITQNISTSDFF
                     EDINRQLGEVKKSVKTDFVGYKGTTDELNVFADRFAHISDNMDHTSNEISIIVEQVAE
                     GAINQAYETEQVASQLNDSIVSLNEVVKKENQGKEQLESSVEIISSGFDNLKSTSQSL
                     NQILTQFSQVRTQGDDLQNRARNVLNIVETVEEIAEQTNLLALNAGIEAARAGEYGRG
                     FSVVAQEIRKLAEGSREAAQSINNSLKAFIENIDDFVLEISNQYNILDKENTKLNSVT
                     ENNLESLNSITRVTNLIIELTEELTAEADNINSIFENIEALSAIAEENSASSQEVSAN
                     VQTYAEEIKRMTENIHEFKKVSIQFSEELERYVI"
     gene            complement(2992..3204)
                     /locus_tag="VK031_10530"
     CDS             complement(2992..3204)
                     /locus_tag="VK031_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HLS54402.1"
                     /translation="MYQSIRAEIMKRQIDLNAKRAFEEMKLEIANELDIKLADLGNIS
                     GTMIKELAEIGQRQLSDEKMEDFDPK"
BASE COUNT         1056 a          567 c          595 g         1162 t
ORIGIN      
        1 gtcatagcaa gaaggtttga aaggctagaa aaattggcag aaagagcagc tgattttgaa
       61 ggggaaattt atcccttagc aggagatatg tctaaggatg aagacataaa aaatacggtg
      121 gaaaagactg ttgaaaaata tggtagggta gatgtagttg taaataatgc tggagtattg
      181 gataagttct tatctgcaga taatatggaa gatgaggtat gggattttgt tatgacagta
      241 aacgtaacag gacctatgaa attaatcaga gaagccatgc cttatatgaa ggaacaaaag
      301 tcaggcaata ttataaatat ttcttctgta ggaggcctat ttggtgccaa gggtggcttg
      361 gcctatgtta ctagtaaaca cgccattata ggtatgacta agcatattgc tgctatattt
      421 ggtgatcaag gcattaggtg taatgcaata gcaccaggaa ctgttgaaac ggagatagga
      481 gggacagtaa aggagcctaa tatgatggta ctagataagg taatgaaaac agctgaagta
      541 tatccagttg tgggagagcc agatgatata ggcaacttaa ttttattctt agcatctgat
      601 gaatctaaat ttataaatgg tgcaaccatc gtaagtgatg gtggatggac tatccactaa
      661 aaacggtaac ccccctagat ctaatcgtag atttaggggg atttaattat tgcatctgta
      721 agatttatcc caatggcctt aaatttttga aatcttttaa tatccatagg gtggtcatga
      781 atgaaattat cggaaaaaag ttttcctaca taataaatat cttagcttaa aatcttctta
      841 tagctgttga aaatctaaaa caaaaagaca tttaactaca ggataaagtt acttctatag
      901 ttaaatgcct aatataaagg attaatacta aaacatgttc atattaaata acatatcttt
      961 ctaattcctc actaaattga atgctaactt ttttaaattc atggatgttt tctgtcattc
     1021 tcttaatttc ctctgcatag gtttgaacat tggcagatac ttcttgagag gaggctgaat
     1081 tttcttcagc tatggctgat aaggcttcaa tattttcgaa tatagaatta atattatcag
     1141 cctctgcagt aagttcctct gttaattcta tgattaaatt agtaactcta gtaatagaat
     1201 ttagtgattc tagattattt tctgttacag aatttaattt agtattttcc ttatctaata
     1261 tattgtactg gttggaaatt tctaggacaa aatcatctat attttcaata aaagccttca
     1321 agctgttatt aatgctttgg gcggcctctc ttgaaccttc tgcaagtttt cttatttctt
     1381 gggctaccac agaaaaacct ctgccatatt caccggctct ggctgcctct ataccagcat
     1441 ttaaagccaa aagattagtc tgctctgcaa tttcctcaac tgtttccaca atatttaata
     1501 catttctagc cctattttga aggtcatctc cctgggttct gacctgagaa aattgtgtca
     1561 ggatctgatt tagactttgg gaggtggatt ttaaattatc gaagcctgag ctaattattt
     1621 ctactgatga ctccaactgc tccttaccct gattttcttt tttaactact tcgtttaagg
     1681 atacaataga gtcattgagc tggcttgcca cctgctcagt ttcataggcc tgatttatgg
     1741 caccttcagc cacttgctcc actataattg atatttcatt ggaggtatga tccatattgt
     1801 cagatatatg ggcaaaccta tctgcaaata catttaattc atcggtggtg cccttgtaac
     1861 cgacaaaatc agttttaacg gattttttaa cctcacctaa ctgcctattt atatcttcaa
     1921 agaaatcaga tgtagatata ttttgagtga tggaaaaatt cttattttta atttcatcta
     1981 aatgagcttt aatatggttt aaaggcagaa aaagccccct actaactaag gccggtatta
     2041 gaaaagatag gcctaagata cttggcaggg ctatcttttt aggcagataa ttaaaaataa
     2101 gaatactagg aactccaaca aataataggt tagctaatgc tattttaact tccatacttt
     2161 taataaggcc tagggacatt aatttattaa atataaagtt tttctcataa tatatttctt
     2221 ccggaaaggt aatggctaat tttaaaaagg tatcagtttg ttcaattgta tctacctgta
     2281 tatcttcttc aaaatgtttg gctgctccct ctagcatgcc aagaaaataa gcaaacatgc
     2341 ctctggagga agaataggtc atatgagctg tatgactgtc tacaggttct atacctaaaa
     2401 ttggtggctg agcccctgga actcttttgg tgactataac gtgaatgtca tacatggctt
     2461 gtaagaagga gtatagattt ttatatctaa aaaaagctgg gtaaacttta gtataggttt
     2521 ttatattgtg atttcccata tttctccaga tttcatctga acttttccct agcttgtccc
     2581 ctatatattc caatatacct aaagctatat tatcatcaat atcctcggta ggggtaaata
     2641 tcctatcagg tgccatacca aagtgggaca aggcttcacc tgtaatttct tctccgtata
     2701 attctttaca tgtttgaacc catgctgaaa caatagtgcc cttcatattt ttcctcctaa
     2761 ataagtgtta gttcttatat accctatcaa tatagtaatc ggcaataata ataaaaaata
     2821 taggttgatt tagtaaatct tgcagaaaag gcttaaattt tagagaaacc ttataaatga
     2881 aaactccttt ccagtaagga attgaaaaat tcaaccagaa aggagttttc atgttagtaa
     2941 ggtcaataac cttattactt ataatttaat gacaggtaac ttaaccatta tctacttggg
     3001 atcaaagtct tccatctttt catctgataa ttgcctttgg cctatttcgg ccagctcctt
     3061 tatcatggtg ccactgatat tgccaagatc ggccagcttt atatctaatt cattggctat
     3121 ttccaatttc atttcttcaa aggccctttt tgcatttaaa tctatttgtc ttttcattat
     3181 ctcagcccta attgattggt acatttagtg ttttactggg ggagatttta atactaggaa
     3241 aaatatgaga ttaactatag aatccatcct ttttttcttc taagattttt aattcttcag
     3301 gtgtagactc ccggcctaga tctttatttt ggtaggggta gcggccaaat tcttctagta
     3361 cttctctgtg aagtttctca
//
LOCUS       DATGSI010000697         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_279291, whole genome shotgun sequence.
ACCESSION   DATGSI010000697 DATGSI010000000
VERSION     DATGSI010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279291"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VK031_10535"
     CDS             complement(<1..943)
                     /locus_tag="VK031_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HLS54403.1"
                     /translation="MFKKAFSILLVLVLVASLMVGCGQNADKPADGGSGTGGGETVYV
                     NIATGGTGGVYYPLGGALAKVYNDKLDNISANSQSTGASVENIGLILDDEAQIAFIQN
                     DITYYAAEGIEMFEDKGKAEDLRGMAIWYPEVIQIVATKGSGIETVEDLKGKKVAVGA
                     PGSGTEANARQILEAHGITYDDIQADYLSFDEASTNLQDGNVDAAFVTAGLPTAAIVE
                     LTTSTDVVIVPIESGVIADLSAEYPFYTEVVIEAGTYKDQAEDVTTTAVMAMLVVHKD
                     MDEDLAYELTKAMFENKDIIDETHDRGKDLQLETALEG"
BASE COUNT          474 a          317 c          208 g          538 t
ORIGIN      
        1 taccttctag agcggtttct agttgtaagt ctttccctct atcgtgagtt tcatctataa
       61 tatctttatt ttcaaacatg gccttagtta attcataagc tagatcttca tccatatcct
      121 tatgaactac taacatagcc attactgctg tagttgtcac atcttcagcc tggtctttgt
      181 aagttccagc ctcaattact acctcagtgt agaatggata ttcagcactt aaatctgcaa
      241 tgacaccgct ttcaattggt actataacta catctgtact agtagttaat tctactatag
      301 cagcagttgg taatcctgct gtaacaaacg cagcgtctac gttaccatct tgaaggtttg
      361 tggatgcctc atcaaaggat aagtagtcag cctgtatatc atcatatgtg attccatggg
      421 cttctagaat ttgtctagca tttgcttctg taccagatcc tggtgctcca acagctacct
      481 ttttaccttt tagatcttcc acagtctcaa tgcctgaacc ctttgttgct actatttgga
      541 ttacctctgg ataccaaata gccataccgc gaaggtcttc tgccttacct ttatcttcaa
      601 acatttcaat tccttctgca gcataataag taatgtcatt ttggataaat gctatctgag
      661 cttcatcgtc taatattaga ccgatatttt ctactgatgc ccctgttgat tgggagttag
      721 cagaaatatt gtctaatttg tcattgtaaa cctttgcaag agctccgcct aatgggtagt
      781 atactccacc agtacctcca gtagctatat taacgtatac tgtttctccc ccacctgtgc
      841 cactgccgcc atcagcaggt ttgtctgcat tctgcccaca ccctaccatt aatgatgcta
      901 ctagcaccaa caccaataga atagagaacg ccttcttaaa catttaaatc ccccttttta
      961 aagtttctac ctattgggaa gttacattaa atttcccaaa taagtagttt acccatatta
     1021 tacaacaaag taaccacata ttccagtaat tttcagaatt tttacttatc acaacttgta
     1081 aagttaggaa aaaattaaaa tttgtatcta tgtaacattt taaagaaaca aaaatctatg
     1141 aattatgaat actaataaac aagattctta gtaagaaaaa attaaatact gcctttaatt
     1201 aaacattatg gaaatatact aaatatacaa aataaaacaa gtaattaaaa tttctaataa
     1261 tatcctattt tatttcaata gaattattat attttgaaaa ctttgaatat ctatataaat
     1321 gaaagtagtt tataagacat tcttcacttg tcttgttaat ttatatcact atcttatagc
     1381 ttttctttat ccagctattc aaaataatcc atcttataaa atttagatgg attattttgg
     1441 gaatctattc atatactatc tagtaatctt ctccaagccc ctaaggtagg gtctaagtac
     1501 ctctggaatt actacactgc catcctcttg ctgatag
//
LOCUS       DATGSI010000698         1740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_45123, whole genome shotgun sequence.
ACCESSION   DATGSI010000698 DATGSI010000000
VERSION     DATGSI010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45123"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..85
                     /locus_tag="VK031_10540"
     CDS             5..85
                     /locus_tag="VK031_10540"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLS54404.1"
                     /translation="MDGDCGGFNLQWAWSSGYVAGTSAAL"
     gene            112..1407
                     /locus_tag="VK031_10545"
     CDS             112..1407
                     /locus_tag="VK031_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLS54405.1"
                     /translation="MIGINRKLRQLDVENKHIRVSLIGAGLMGKGMVSQMMLMKGMIP
                     ALVASNKLEDAIAAYTLAGVKKEDIIIGRSLNQVNKAMEMGKLVATDIAEYATEANLV
                     DVVVDATGVPNVGAKVAIDAIENGKHMVMLNVEADATIGPILNKLAKDAGVVYTGSAG
                     DEPGAVMELFDFADAIGFDIIAIGKGKNNPIDYEATPDSVLEKSKRFNIKPLRLASFV
                     DGTNTMVELTSMANATGFVPDIQGGHGPKAQVEDLAQIFSLKEDGGILDKSGIVDYVK
                     GIAPGVFAVVTTDLPQIKAELEMVAMGKGPNYVLYRPYHLVSLETPITIARAAIYNEA
                     TIAPMAGPVAEVVTRAKRDLKAGEYLDGIGEYTVLGSIDTYENARRNNLVPIGLISPS
                     TKVKRDIKKGELISLDMVELDTNTLIYQLKEQQDRIFSK"
     misc_binding    1514..>1740
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          598 a          249 c          433 g          460 t
ORIGIN      
        1 ggatatagac ggagattgtg gtggatttaa cctgcagtgg gcctggtcat ctggttatgt
       61 ggcagggact agtgcagcac tttaattttc cagctaatta gggaggaaat tatgataggt
      121 ataaatagaa aacttagaca attagatgta gaaaataaac atataagggt atctttaatt
      181 ggagcaggat tgatgggtaa gggcatggtg agccaaatga tgttaatgaa gggaatgata
      241 ccagccctag ttgccagtaa caaactggag gatgccatag cagcttacac cttagctggc
      301 gttaaaaagg aagatataat aatcggaagg agcttaaacc aggttaacaa ggctatggaa
      361 atgggcaagt tagtagccac agatatagct gaatatgcta cagaggccaa tctggtagat
      421 gtggtagtag atgcaacagg ggtaccaaat gtgggggcta aggtggcaat agatgctata
      481 gaaaatggaa aacatatggt tatgctaaat gtagaggccg atgcaaccat agggcctatt
      541 ttgaataagt tggccaagga tgcaggggtg gtatatacag gttcagcagg tgatgaacct
      601 ggagctgtta tggaactttt tgattttgcc gacgccatag gatttgacat aatagctata
      661 ggaaagggta aaaacaaccc tatagactat gaagcaactc cagatagtgt tttggagaaa
      721 tcgaaaaggt ttaatataaa gcctttaaga ctggcctctt ttgtagatgg gactaataca
      781 atggtagaat taactagtat ggccaatgca acaggttttg tgccagatat tcagggagga
      841 catggaccta aagcccaggt ggaggatttg gctcaaattt ttagcctaaa agaagatgga
      901 ggcatattag ataaatctgg aattgtagat tacgttaagg ggatagctcc tggagttttt
      961 gctgtggtaa ccacagatct tccacagata aaggcagagc tggaaatggt ggccatggga
     1021 aaggggccaa attatgtctt atataggccc tatcatttgg taagccttga aacacctata
     1081 accatagcca gagcagccat atataatgaa gcaaccatcg ctcctatggc aggaccagtg
     1141 gcagaagttg taaccagagc taaaagagat ttaaaggctg gtgaatattt agatggtata
     1201 ggtgaatata cagtattagg aagtattgat acatacgaaa atgcaaggag gaataacttg
     1261 gtaccaatag gcctgatttc tcccagtacc aaggtaaaaa gagatataaa aaagggtgag
     1321 ctaataagtc ttgatatggt ggaactggat accaacaccc ttatatatca attaaaggag
     1381 cagcaagata ggatatttag caaatagatt aaatataata tctttaattt taaatcctaa
     1441 aagatgcaag agctgaattt agccaaggct tgacaaatta ttaataatta agtatattaa
     1501 taactaagtg aaaatgtatg cattgacaag gacaagtagc aaggcccacc tattacagag
     1561 agataatcag ttggtgaaag attattatgg gcagctttgt gaaaatcacc ttggagcata
     1621 atacctgaaa ggccatggat tagttttttg atagaatgat tactgttgtc tagtagggta
     1681 ttagggtttg ggcccttatc ccaattaatg aagagacaag atacttcttg taattggggt
//
LOCUS       DATGSI010000699         1165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_45539, whole genome shotgun sequence.
ACCESSION   DATGSI010000699 DATGSI010000000
VERSION     DATGSI010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45539"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VK031_10550"
     CDS             <1..405
                     /locus_tag="VK031_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54406.1"
                     /translation="IGLSRKNGYWMFKGRINYNQNNKELYKDFSISAIPPKDLVNYNE
                     LVLPWEAVKRKIPEAIDVYSSPNEDFIVVVTHGDLQIFEIANGQILDSKPVKTIDLPK
                     NASIIMAEWATGRYANLWEEEVVRQDGEVIEE"
     gene            441..749
                     /locus_tag="VK031_10555"
     CDS             441..749
                     /locus_tag="VK031_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLS54407.1"
                     /translation="MREFLEYMDYAKIFIYISIGTMILTFISQHFLKRYKNAKYIPGL
                     ILLGIGFYGLISLIRATTVAKGISSLMIFVVGIGSGIIGLLTGLIINIYKRDNNVKSS
                     "
     gene            complement(814..1062)
                     /locus_tag="VK031_10560"
     CDS             complement(814..1062)
                     /locus_tag="VK031_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HLS54408.1"
                     /translation="MIGLHYVYMVKCSDNTLYTGYTVDLEARINTHNKGLGAKYTRGR
                     LPVELKYFEIFESKSKALKREYEIKQLSRKSKWKLINK"
BASE COUNT          393 a          173 c          211 g          388 t
ORIGIN      
        1 ataggcctat ctaggaaaaa tgggtattgg atgtttaagg gcaggataaa ttataatcaa
       61 aacaataaag aattgtacaa ggactttagt attagcgcaa taccacctaa agatttagta
      121 aattataacg agttggtttt accctgggag gcagttaaga gaaagatacc agaggctata
      181 gatgtatatt catcaccaaa cgaagacttt atagttgtgg taacccacgg tgatttacaa
      241 atatttgaga ttgcaaatgg gcaaattctt gattctaagc cagtaaaaac aattgatttg
      301 cctaaaaatg cttctattat tatggcagaa tgggcaacgg gaagatatgc taatctttgg
      361 gaggaagagg tagttaggca ggatggagaa gtaatagaag aataaattta tcttatcata
      421 aatgtagggg gggtgagcag atgagagagt tcttagagta tatggattat gccaagattt
      481 ttatttatat atctattgga actatgattt tgacctttat ttcccaacac tttcttaaaa
      541 gatataaaaa tgcaaaatat attcctggct taatcttatt agggataggt ttttatggcc
      601 taatttcctt aatcagggct actactgtag ctaagggaat tagtagcctt atgatatttg
      661 ttgtagggat aggtagtgga attataggct tgttaacagg acttataatt aatatatata
      721 aaagggataa taatgtaaaa tcaagctaat aaactggcac cttatcaaaa agtataaggt
      781 gccagtttaa attaatggcc aaggttagaa tttttattta ttaatcagct tccatttact
      841 tttcctgctt aattgtttaa tttcatattc cctttttaag gccttgcttt tactttcaaa
      901 gatctcaaaa tactttaatt caactggtag acggccccta gtatatttgg ctccaagccc
      961 cttgttatga gtatttatcc ttgcttctaa atcaactgta taaccagtat ataaagtatt
     1021 atcactacat ttgaccatat atacataatg caagccaatc acctactgct taaaagttct
     1081 tttaaaatcc tggtgaccac accataagaa aagcctcttc tttggagaaa gccaccaagt
     1141 tttctatata tctgatctct ttcat
//
LOCUS       DATGSI010000700         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_45715, whole genome shotgun sequence.
ACCESSION   DATGSI010000700 DATGSI010000000
VERSION     DATGSI010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45715"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VK031_10565"
     CDS             complement(<1..242)
                     /locus_tag="VK031_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLS54409.1"
                     /translation="MIRFENVTKKFGNFVAVDNLKVNFKPGETNVLIGPSGCGKTTTL
                     RMINRLIEPTKGNILIDGQDIGKIDPVELRRNIGYV"
     regulatory      complement(340..470)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(528..1226)
                     /locus_tag="VK031_10570"
     CDS             complement(528..1226)
                     /locus_tag="VK031_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLS54410.1"
                     /translation="MKKRLLIVDRDSIFSKGLKYSLEQLNYEVDRLFHGTAMLQVLEN
                     RERDYDMFIVDIDIRDMDGLRLIKDVRNLTHVPLIATGQAEQDITRIMALENGADDFL
                     DKPYNKDELKARIRAIFRRIEYEESKAEINHIIQVKDFTVDTLKRKVLAAGKQLYLTA
                     TEFQIFLVLISNPGITLTREEILENIWGADFFCDIRSIDVHITKIREKLDDTIGKPEY
                     IGTKWGEGYFFNNL"
     gene            complement(1316..3220)
                     /gene="selB"
                     /locus_tag="VK031_10575"
     CDS             complement(1316..3220)
                     /gene="selB"
                     /locus_tag="VK031_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HLS54411.1"
                     /translation="MKHIIIGTAGHIDHGKTSLIKALTGRETDRLKEEKKRGISIELG
                     FTYFDLPNGQRAGIIDVPGHERFIKNMLAGVIGIDIVLLVVAADEGPMPQTMEHLAIL
                     DLLGIERGFIVLSKTDLVEEEWLELVEEEVRDKVRGTFLEKSPIVRVSSTKKQGLDQV
                     IHLLEKAAKDIEDKEINQMPRLPVDRSFIVPGFGTVVTGTLLSGSLKIGDEVEAYPSG
                     QISRIRTLQVHEEDAEIAYAGQRVAMNLAGLKREDVNRGSVIAPVGSMEKTRMLDVKI
                     KLLDSLDRPIKNRSRLRIYLGTKELLARLVLLDRDILNPGEEAYAQLRLEEELVAQRK
                     DKFIVRFYSPMFTIGGGVVLEANPRKKRRFNKKDLEELRIKEQGTSLEILEKIIEDRS
                     PSFPTIKDLSLYTSNLEETLIKDIDKLEEAGRLISFSLTKDKYIIHKNYFKGLREKIE
                     KDLKAYHKKYPLRKGRSKEEIRGKYLKDASTRLGEAILDRLIEEGYIKQDMEHIRLTD
                     FTLSYNEEQKKIKEKIISHLQAQAYMPPKREELIEEIPGDKREIQEVFNSLVNKELIR
                     LNEEVFILKDLYHKAVEKIKDYIEENGSITVAEFRDLLDSNRRNALALLEYFDQEKIT
                     KREGDKRLLV"
     gene            complement(3217..>4102)
                     /gene="selA"
                     /locus_tag="VK031_10580"
     CDS             complement(3217..>4102)
                     /gene="selA"
                     /locus_tag="VK031_10580"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HLS54412.1"
                     /translation="EIGGSFRIPDVMEESGASLVAVGTTNKTHLEDYERAITEETAAL
                     LKVHTSNYRILGFTSSVDAKDLSSLKDKYQLPLIEDLGSGILIDLSKYGIDYEPTVQD
                     SLKKGVDVVTFSGDKLLGGPQAGIIVGKKKYIEEMKRNPLTRALRVDKFTLTALETIL
                     KLYLEEEKAVEKIPTLRMLTLGLEKIDKRAKKLFKEIKDLNLDKDLEIAIEDSYSEVG
                     GGSMPLVKLDSRSLVLSCKSISSQELEKRLRSLKLPIITRLYRDKIYMDLRTIKEEEI
                     PLISQGIKEVFKIKESQL"
BASE COUNT          968 a          947 c          672 g         1515 t
ORIGIN      
        1 attacataac caatatttct cctaagttcc actggatcta tcttgcctat gtcttgtcca
       61 tctattaata tattgccttt tgtaggctct attaatcgat ttatcatcct aagggtggtg
      121 gtctttccac aaccactggg acctattagg acgttggttt ctcctggctt aaagtttacc
      181 ttaagattgt ccactgccac aaagttgcca aactttttag ttacattttc aaatcttatc
      241 atatgcatac ctcctttttt atttgctaca gtttatctat tccttttttt cacacaaaaa
      301 aactatggag agccatagtt tttcaaaaaa acttaatacc atcactctct tccatgctta
      361 cgaggttagc tgccgggttc gggctgaaga gtagccctac ttcttaaaga agattcaccc
      421 caatatattg gttcccccgc tctaaatttt agattcagca agcttgaaat ataagcaaat
      481 attaaattat gatcttattt tatcaaatat aactaagttt gtccaattta taggttgttg
      541 aagaaataac cttcacccca tttggttcct atgtattcag gcttacctat ggtatcgtcc
      601 aacttttctc taatctttgt tatgtggaca tctatggatc ttatatcaca gaaaaagtct
      661 gccccccaaa tgttttctag tatttcttcc ctggtcaagg taatgcctgg attagatata
      721 aggaccaaga atatttggaa ttctgtggct gtaagataaa gctgtttgcc agctgccaga
      781 acctttcttt taagtgtatc tacagtaaag tcctttacct ggattatgtg gttaatttca
      841 gccttagatt cctcatattc tatccgtcta aagatggccc tgattctagc ctttagttcg
      901 tccttgttat agggtttgtc taaaaagtca tctgccccat tttccagggc cataatcctg
      961 gttatatcct gctcagcctg acctgtggct ataagtggta catgggtaag gtttcttaca
     1021 tctttaatta gccttaatcc atccatatcc cttatatcta tgtctacaat aaacatatca
     1081 tagtctcttt ccctattctc taaaacctgc agcatggctg taccatgaaa cagacggtcc
     1141 acctcatagt taagctgctc caggctatat ttcaatccct tgctaaaaat actatcccta
     1201 tcaactatta ataatctttt cttcatactt tcacccctgt tttgatagta tatttagttg
     1261 ggagccaggc tccctgttta attttagtag atataattta aaaggaataa tcaatttata
     1321 ctagtagtct tttatcccct tccctttttg ttattttttc ttgatcaaag tattctaata
     1381 atgctaatgc atttctccta ttggaatcaa gtagatctct aaattctgcc acagtaatgg
     1441 agccattttc ttctatatag tcttttattt tctccactgc cttatggtat aggtccttta
     1501 agataaatac ttcttcattc agccggatta gctccttatt taccagggag ttaaatactt
     1561 cttggatttc tcttttatct cctggaattt cttctattaa ttcttccctt ttaggaggca
     1621 tataggcttg ggcctgtagg tggcttatta ttttttcttt gatttttttc tgctcctcat
     1681 tgtagcttag tgtaaagtct gtaagtctta tatgctccat atcctgcttt atataacctt
     1741 cttctattag ccggtctaat atggcttccc ccagcctggt ggaagcatct ttcaagtatt
     1801 tgcctctgat ttcttccttg cttcttccct ttcttagagg atattttttg tgataggctt
     1861 ttaagtcctt ttctatcttt tctctaagcc ctttaaagta gtttttatga attatatatt
     1921 tgtccttagt taggctaaaa cttataagcc taccagcctc ctccagtttg tctatgtctt
     1981 taataagcgt ctcttctaaa ttggaggtat ataggcttaa gtccttaata gttggaaagc
     2041 taggacttct atcttcaata atcttttcca atatttctaa ggaagtcccc tgttccttaa
     2101 tccttaactc ttccaagtct tttttattaa atcttctttt cttccttgga ttggcctcta
     2161 aaactacccc acctcctatg gtaaacatag gtgagtagaa gcggacaata aatttgtcct
     2221 tcctttgggc caccagctct tcttccagcc ttagctgggc ataggcctct tcacctggat
     2281 ttaatatgtc cctatctaat agaaccagtc tggccagtag ctccttggtt cccagataaa
     2341 tccttaacct gcttctgttt ttgatgggcc tgtctaggga gtctaataac tttattttta
     2401 catccagcat cctggttttt tccatggatc ctactggggc tattacacta cctctattca
     2461 catcttccct tttaaggcca gccaggttca tggccaccct ttggcctgca taggctattt
     2521 ctgcatcttc ttcatggacc tgcagggtcc tgattctaga tatttggcca ctgggatagg
     2581 cctccacctc atcacctatt tttaagctgc ctgacagtag ggtaccggta actacagtgc
     2641 caaagcctgg tactataaaa gagcgatcca ctggtagcct aggcatttgg ttgatttcct
     2701 tatcttctat atccttggct gccttttcta aaaggtggat tacctggtcc aggccttgtt
     2761 tttttgtgga agatacccga actatgggac ttttttccaa gaaggttccc cttaccttat
     2821 ctcttacctc ttcttccacc agctccagcc attcttcctc caccaggtca gttttactga
     2881 gaactataaa gcctctttct atgcctagca ggtctaaaat agccaagtgc tccatggtct
     2941 gaggcatagg cccttcatcg gctgccacta ccaaaagcac tatatctatg cctataactc
     3001 cggccaacat atttttaata aacctttcat ggccaggcac atctatgatt cctgcccttt
     3061 ggccatttgg caggtcaaaa taggtaaagc ctagttctat ggagatgccc ctcttttttt
     3121 cttcctttag cctgtctgtt tccctgcctg ttaaggcctt aatcaggcta gtcttaccat
     3181 ggtcaatatg gcctgctgtt cctataatta tatgtttcat agctgactct ccttaatctt
     3241 aaatacctct tttattccct ggctaattag ggggatttct tcttccttta tggtcctaag
     3301 gtccatatat atcttatccc tatatagtct ggttataatt ggtagtttga gacttcttag
     3361 cctcttttcc aattcctggc tgcttatgct tttgcagctt aaaaccaggg acctgctgtc
     3421 tagtttcacc agaggcatgg agcctcctcc tacttctgag tagctatctt ctatagctat
     3481 ttccagatcc ttgtccaggt ttaggtcctt tatctcttta aaaagtttct tggccctttt
     3541 atctattttt tccaatccta aggttaacat ccttagggta ggtatttttt ctactgcctt
     3601 ttcctcctcc aagtaaagct ttaggatagt ttccaaggct gttaaggtaa acttatccac
     3661 ccttagggcc ctggttaagg gatttctttt catttcttct atatattttt tcttacctac
     3721 tattatacct gcctgagggc cacctaagag cttatctcca ctgaaggtta ccacatctac
     3781 tccctttttt aaggagtcct ggactgtagg ttcatagtct attccgtatt ttgacaggtc
     3841 tataaggatt ccactgccta aatcttctat aagtggcagc tggtatttat cctttagact
     3901 acttaaatcc ttggcatcta cagaagaggt aaagcctagg atccggtagt tggaagtatg
     3961 gaccttaagc agggctgctg tttcttctgt aatggccctt tcatagtctt caaggtgggt
     4021 cttattggtg gttcctactg ccaccaggct ggctccactt tcctccatta catctggaat
     4081 cctaaaggag cctcctattt ct
//
LOCUS       DATGSI010000701         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_280494, whole genome shotgun sequence.
ACCESSION   DATGSI010000701 DATGSI010000000
VERSION     DATGSI010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280494"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VK031_10585"
     CDS             complement(<1..355)
                     /locus_tag="VK031_10585"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HLS54413.1"
                     /translation="MPYKLPELKYSYDALEPHIDQLTMETHHSKHHQAYVDNANKALE
                     GHENLKAMKVEDLLKSLDEVPEEIRTALRNNAGGHYNHSLFWELMSPNGGGQPGGELA
                     EKIEEDLGGFEKFKED"
     gene            complement(457..1032)
                     /locus_tag="VK031_10590"
     CDS             complement(457..1032)
                     /locus_tag="VK031_10590"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLS54414.1"
                     /translation="MDIIMIRHGQSEANLAKIFGTEDTRLSKEGIRQIKRAKKHIDTL
                     SFSKVYVSPFARTKETLHHLGFQAKEDKRIQEYDFGIFSGLTNEEIEKKYPLEYQEWI
                     ENPMDYKIPEGESLQIVYRRVKNFLEEIIEKNENVLLVTHAGIIRLAFCWVFNKPEYF
                     FKFKVTNGSVNIITVNEDNFKYIKQVNFSPR"
     gene            complement(1023..1760)
                     /gene="cobS"
                     /locus_tag="VK031_10595"
     CDS             complement(1023..1760)
                     /gene="cobS"
                     /locus_tag="VK031_10595"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HLS54415.1"
                     /translation="MIKGFILSLQFLTRLPIHIPIDFNRENLRKSTFFYPLVGMILGI
                     LSFIPYYFLSNYNREIAAFLTVLFIIILTGGLHLDGLADTADGFFSNKDKEKTLEIMK
                     DSRIGAFGVLSLILLILFKYIIISSIKEKTLLILMLSLGNSRLSVLFQIAYKKTARPD
                     GLGRMLNQSQPGPYVLLGSFLYVIFVIFINPYYTIPLISSLVTGKLTALYSKRKIDGL
                     TGDVYGAIIELSEAISLLAFWGVIEWI"
     gene            complement(1757..2293)
                     /gene="cobU"
                     /locus_tag="VK031_10600"
     CDS             complement(1757..2293)
                     /gene="cobU"
                     /locus_tag="VK031_10600"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HLS54416.1"
                     /translation="MIRLITGGARSGKSSFAEGQFIDKEDVVYIATSRVKDKEMEERV
                     QLHQKSRPDIWRTFEGNYNLVDAIGKEKYYLLDCITVLSSNIMFDLSRGVEYIDYKLQ
                     EKIEGKILKELESLIRTVKDKGYELNLVTNEVGYSIVPEHHISRVFRDIQGRVNQKVA
                     ALSDEVYLVSCGIPVKIK"
     gene            complement(2307..>2610)
                     /locus_tag="VK031_10605"
     CDS             complement(2307..>2610)
                     /locus_tag="VK031_10605"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HLS54417.1"
                     /translation="PIVMDGFISSTAALCAVKLNPLVKEYVFTSHLSDEPGSKYIFNE
                     LGLDAMLNLKMRLGEGSGCPIAFQIIDMALYTQENMGTFQEATIDSTVLIDIREEE"
BASE COUNT          749 a          539 c          375 g          947 t
ORIGIN      
        1 agtcttcttt aaacttttca aatccaccta agtcttcttc aattttttca gctaattctc
       61 cacctggctg tcctccacca ttaggtgaca taagctccca aaagaggctg tggttatagt
      121 gtccacctgc attatttctt aaggcagttc taatttcttc tggtacttca tctaaggact
      181 tcaacaagtc ctccaccttc atagccttta gattttcatg accttctagg gccttatttg
      241 cattgtccac ataggcctga tggtgctttg agtggtgggt ttccattgtc aattgatcaa
      301 tatgaggttc tagggcatca taagagtatt ttaattctgg taatttatat ggcattatat
      361 ctctccttta aatttctttt tcttgtacta tatatattcc cattttttta attgataaac
      421 attatcattt aattatttta aaaagtccta gattttttac ctaggactaa agttaacttg
      481 ctttatatat ttaaaattat cttcatttac cgtaataata ttcacacttc cattagttac
      541 cttaaattta aaaaagtatt ctggtttatt gaaaacccaa caaaatgcca gccggataat
      601 tcctgcatgg gtcactaata agacattttc atttttttct attatttcct ctaaaaagtt
      661 tttaacccgt ctataaacta tttgtaagct ttctccctca ggtattttat aatccatagg
      721 gttttctatc cattcttgat actctaaggg atattttttc tctatttcct catttgttag
      781 gcctgaaaaa attccaaaat catactcttg gatcctttta tcttctttgg cctgaaaccc
      841 taaatggtgt aaggtttctt ttgtccttgc aaagggactt acataaacct tagaaaagct
      901 taaagtgtct atatgtttct tggccctttt tatctgcctt ataccttctt tagatagtct
      961 ggtatcttca gtaccaaata ttttagccag gttagcttcc gactggccat ggcggatcat
     1021 tattatatcc attctattac cccccagaaa gccaaaagtg atatggcttc acttagttct
     1081 ataattgccc catacacatc tcctgtaaga ccatctattt tccttttact ataaagggct
     1141 gttagcttac ctgttacaag ggagcttatt aggggaattg tgtagtaagg gttaataaag
     1201 attacaaaaa ttacatataa aaaacttccc aataatacat atggtccagg ctgactttga
     1261 ttaagcatcc tgcctagtcc atctggcctt gcagtttttt tataggcaat ttgaaatagt
     1321 actgataatc tactgttacc cagagaaagc atcagaataa gcaatgtttt ctcctttatg
     1381 ctagatatta ttatatattt gaataaaatt aataaaataa ggcttaatac tccaaaggcc
     1441 cctatcctgc tatccttcat tatttctaag gtcttttcct tatctttgtt ggaaaaaaat
     1501 ccatcggcag tatctgccag accatctagg tgtagaccac cagttaaaat aattataaat
     1561 agcactgtta gaaaggcggc aatttcccta ttgtaatttg ataagaagta gtaggggata
     1621 aaggataata ttcctaaaat catacctaca agaggataaa aaaatgtact tttccttaag
     1681 ttttccctat taaaatctat aggaatatga ataggcagcc tagttaaaaa ttgtaggctt
     1741 aagataaatc cttttatcat ttaatcttca ccggaatccc acaagatact agatacacct
     1801 catcacttaa ggcagccacc ttttgattta cccttccctg gatatcacga aaaaccctgg
     1861 atatgtggtg ttctggtact attgaatagc ctacctcatt ggtgaccagg tttaattcat
     1921 atcccttatc ttttaccgtt ctaattaaag attccagttc ttttaagatt ttcccctcta
     1981 ttttttcctg caatttgtag tctatatatt ccacgcccct acttaaatca aacattatat
     2041 tggaacttaa gacagttata caatcaagta aataatattt ctccttacct attgcatcaa
     2101 ccagattata atttccttca aaggtcctcc aaatatcagg ccttgatttt tggtgaagct
     2161 gaaccctttc ttccatttcc ttatctttaa ctctggatgt ggcaatatac accacatcct
     2221 ccttatctat aaattgacct tccgcaaagc tactttttcc acttctggcc ccacctgtta
     2281 ttagcctaat catactacca cctatcctat tcttcttccc taatgtctat aagcactgtg
     2341 ctatctattg tggcctcttg gaaggttccc atgttttctt gggtatatag ggccatatct
     2401 attatttgaa aggctatggg gcagcctgat ccctctccca gcctcatttt tagatttaac
     2461 atggcatcta ggcccaattc attaaagata tatttgcttc caggttcatc agataggtgg
     2521 gaagtaaata catattcttt aactaaagga ttcagtttta ctgcacacag ggctgctgtt
     2581 gatgatataa atccgtccat aactataggc
//
LOCUS       DATGSI010000702         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10473
            k141_280666, whole genome shotgun sequence.
ACCESSION   DATGSI010000702 DATGSI010000000
VERSION     DATGSI010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957244
            Sequence Read Archive: SRR5690625
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 19:26:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,104
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 6 of 12
            Pseudo Genes (internal stop)      :: 5 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280666"
                     /isolate="SMAG_U10473"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..741
                     /gene="sdaAB"
                     /locus_tag="VK031_10610"
     CDS             73..741
                     /gene="sdaAB"
                     /locus_tag="VK031_10610"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HLS54418.1"
                     /translation="MKDYNSFDILGPVMVGPSSSHTAGAARLGKIAKEIADKGFYEVE
                     FVLHGSFSMTYKGHGTDKALVAGILGMEPDDENIKNSFNIASDQGINFRFSAEDLGYE
                     HPNTVKIIFKYKDRNDFYIIGSSIGGGSIVITNINGIKIEFTGDKPTILLRYEDRKGV
                     ISEISSILANNDLNIATMKVTREKNIATMVCEVDTKLSENVIEEISSMKPMLYMKFIN
                     PVKR"
     gene            746..1618
                     /gene="sdaAA"
                     /locus_tag="VK031_10615"
     CDS             746..1618
                     /gene="sdaAA"
                     /locus_tag="VK031_10615"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HLS54419.1"
                     /translation="MFNTAAELLEQCKAVNKSISHIVMDKEVTGTGLTLKEVKEKMGD
                     ILKVMKKSATASLDEEKISVSGLTGGNAKRLNDYSKKGKTLSGPFINHAMAMAFSTSE
                     VNASMGKIVAAPTAGAAGILPAALLSAQEKLNLSDQDMIRGLFTAAGVGEIIAKNATI
                     SGAEGGCQAECGAAAAMAAAAIVEMSGGSPKASLDAAGFALTNILGLVCDPIAGLVEY
                     PCALRNASGVINAFISADMALAGVQALIPFDEVVDAMYQIGKSMPEDLRETAQGGLAD
                     TVTGRNYAEKFHKQ"
     gene            complement(1652..>2078)
                     /locus_tag="VK031_10620"
     CDS             complement(1652..>2078)
                     /locus_tag="VK031_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HLS54420.1"
                     /translation="DAQLAERYVRACSKVGAIVSGLMAIIFFFFGGLIASLYTNNSEV
                     VVEAGKVLKLVAFIQPFQSSQLIVSGGLRGAGDTVWALVASFVGILVVRVILAYYFVL
                     SVGMGLIGAWLAVLIDQFIRWIMITLRFRTEKWKYITIR"
BASE COUNT          684 a          390 c          426 g          578 t
ORIGIN      
        1 tgtcttctta tttgctaaaa tacaatacaa agaatataat taaagatgat tatactttta
       61 tggagggata gaatgaaaga ttataattct tttgatatat taggtcctgt aatggtaggc
      121 ccatccagct cccatacagc aggagctgcc aggttaggaa aaattgctaa agaaattgca
      181 gacaagggat tttacgaggt ggaatttgtg cttcacggtt ccttttccat gacctataag
      241 ggccatggca ctgataaggc cttagtagca ggtatattag gtatggaacc tgatgatgaa
      301 aatattaaaa attcctttaa tatagccagt gaccagggca ttaattttag atttagtgca
      361 gaggatcttg gttatgagca ccctaatact gtaaagataa tatttaaata taaagataga
      421 aatgattttt acataatagg ctcttccatt gggggaggca gcattgtaat aacaaatata
      481 aatggaatta agattgagtt tactggagat aagcctacca tactactaag gtatgaggat
      541 aggaagggtg ttataagtga gataagctct attttggcca ataatgattt aaatattgcc
      601 acaatgaagg tcaccaggga gaaaaatata gctaccatgg tctgcgaagt agacacaaaa
      661 ttaagtgaaa atgttataga agagatttca agtatgaagc ctatgctcta catgaagttt
      721 ataaatcctg taaagaggtg aagtcatgtt taatacagct gctgaattat tagaacaatg
      781 taaggcagta aacaaaagta tttcccacat agttatggat aaagaagtca ctggcactgg
      841 ccttactctt aaggaagtta aggaaaaaat gggagacatc ttaaaggtta tgaaaaaatc
      901 agctacagct tcattagatg aagaaaaaat atcagtatca ggtttaaccg gtggaaacgc
      961 caaaagactt aatgactata gtaagaaagg gaaaacttta agcggcccct ttattaatca
     1021 tgccatggct atggcctttt ccacatctga agtaaatgcc tccatgggaa aaatcgtagc
     1081 cgcccctact gcaggagcag caggtatttt accagcagcc ctcctgtcag ctcaagaaaa
     1141 attaaattta tcagatcagg atatgattag aggtctcttt actgctgcag gagttggaga
     1201 gattatagcc aaaaatgcca ctatttcagg ggcggaaggc ggttgtcaag ccgaatgtgg
     1261 tgctgctgcg gccatggctg ctgcagccat agtagaaatg tctggcggtt cccccaaggc
     1321 ttcccttgat gctgcaggct ttgccttaac aaatatttta ggactagtct gtgatcctat
     1381 tgccggttta gtagaatatc cctgtgcctt aaggaatgct tcgggagtga taaatgcttt
     1441 tatatcagcc gatatggcct tggctggggt tcaggccctt attccctttg atgaggtagt
     1501 agatgccatg tatcagattg gcaaatccat gcctgaggat ttaagagaga cggcccaagg
     1561 tggattggca gacactgtaa caggtaggaa ttatgcagaa aaattccata aacaataaag
     1621 cttaataaaa aaacagccct ggggctgttt tttatcttat ggtaatatac ttccattttt
     1681 ctgttctaaa tcgtaaggta atcattatcc acctaataaa ttgatctatt aaaactgcca
     1741 gccaggcacc tattaatccc atacctacgc taaggacaaa gtaataagcc aaaatcaccc
     1801 tgactactag tattccaaca aaactggcca ccagtgccca tacagtatct cctgcccccc
     1861 tgagtccacc agatacaatt aactgcgaac tttggaaagg ctgaataaag gccaccaact
     1921 ttaatacctt tccagcttcc accaccacct cactattatt ggtatacaat gaggctataa
     1981 gccctccaaa gaaaaagaat attatagcca ttaatcctga tactatagct cctaccttac
     2041 tacaggccct aacatatctt tctgccaatt gggcatca
//
