LOCUS       DATGUK010000001         3173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_349755,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000001 DATGUK010000000
VERSION     DATGUK010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349755"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VK103_00005"
     CDS             <1..539
                     /locus_tag="VK103_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HLQ81888.1"
                     /translation="TMAHWLHDVLVDPVTGLVRDGIHTDGTLVERTYTYGQGVVLGLE
                     VELAHRTGDRLHVDRVATLVAAVAGRLCEGGVVTDGGGGDGGLFNGILLRHLAVVAGE
                     LPGRDATATQARATARRVVLASADAAWHHRWRRSDGPVFGHLWTRPAPAPSPLVPERD
                     LSVQLSGWMAMEAAARLH"
     gene            611..1594
                     /locus_tag="VK103_00010"
     CDS             611..1594
                     /locus_tag="VK103_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLQ81889.1"
                     /translation="MTVRVGIAGAGGISRSHLAGLAATEDATVTVVQDLDAERAAEVA
                     AGCSATATTSLDELLDDVDAVFVCTPTLAHRPIVEQAAARGRAVFCEKPLAPSLAEAE
                     AMADAVAAAGVVNQVGLPLRRRGPFPVLRHLLADPENGPLLAIAMSTVSRSRDRVLAG
                     WRGNSAQAGGGMLIEVGFHDIDLLQWLAGPIGAATGQVTPGTHAGIEDAATVALGFEG
                     GGSASLTVAWNAAPVPGQSRRIHVVLDRAEYLLEADGQHQRLVADGPGDRHQEWDADG
                     IRGLTTELGLESNPQAAFVRAVRDGGPASPTLADGVEVHRVLDRVYRAALG"
     gene            1723..2526
                     /locus_tag="VK103_00015"
     CDS             1723..2526
                     /locus_tag="VK103_00015"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLQ81890.1"
                     /translation="MDEQRRRSARWREDYLRDGFLVVEGVLDAAELATLGAALERIEQ
                     RVAEESLPPRLRQYVTLERDQDHAGSADTDKISNIMELPLFDPVFRDLIINASVLDIL
                     EALFGSSEFSFHNYKAICKMPGGAASFRWHRDLPYLPHTSSNLITCMLCIDPMTTDNG
                     ATVVCPGSHRIADDRIDAGDKDIAEAEVPAERLTIECPAGSAVLFDTNIVHGGGPNRS
                     DRPRRNAIGIWAGPDVHPTTASRYAYEFVMPRSTDPQRQLQVQQTFGTA"
     gene            2650..>3173
                     /locus_tag="VK103_00020"
     CDS             2650..>3173
                     /locus_tag="VK103_00020"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6807 family protein"
                     /protein_id="HLQ81891.1"
                     /translation="MRFVIDASSHPRQSPLIRVSPEQGRGGQHPTALRDEATGEVVAV
                     QHDEGTGELSFVVPGQLAAGETRTLTATDEPVTAAHPTTAVVKLDRVVLKVGGERFAD
                     YIVAGGRRPYFWPVVSATGSSVVRGQGSSDHPHHTGLAISYGGHSEDGSTNIWSDWDE
                     PPYGPGGRMVHRGFR"
BASE COUNT          451 a         1161 c         1121 g          440 t
ORIGIN      
        1 tgacgatggc gcactggttg cacgacgtgc tggtcgaccc ggtgaccggg ctggtccggg
       61 acggcatcca caccgacggc accctggtgg agcggaccta cacctacggt cagggcgtgg
      121 tgctggggct ggaggtcgaa ctcgcccacc ggaccggtga ccggctccac gtcgatcggg
      181 tcgcaacctt ggtggccgcg gtcgccgggc ggctgtgcga gggcggcgtg gtcaccgacg
      241 gcgggggcgg tgacggaggc ttgttcaacg gcatcctgct gcggcacctc gccgtggtgg
      301 ccggtgagct gccgggccgc gatgccaccg cgacgcaggc ccgggccacg gcacgccggg
      361 tggtgctcgc ctccgccgat gccgcatggc accaccggtg gagacgttcc gacggtccgg
      421 tgttcggaca cctgtggacc cgaccggccc cggcaccgtc accgctggtc ccggagcggg
      481 acctgtcggt gcagctcagc ggctggatgg cgatggaggc ggccgcccgc cttcactagg
      541 gtgaccccga cccgtggccg cccacaatgg tgaccacgca cgttcctcgg catcgatgaa
      601 gggaccagcc atgactgttc gagtcggcat cgcaggggcg ggtggcatct cccgatcaca
      661 cctggccggt ctggccgcga ccgaggacgc cacggtcacc gtggtgcagg acctcgacgc
      721 ggagcgtgcg gccgaggtcg ccgccggctg ctcggccacc gccaccacct cgctcgacga
      781 gttgctcgac gacgtcgacg cggtgttcgt gtgcacaccg accctggcgc accggccgat
      841 cgtcgagcag gccgcggccc gtggacgggc ggtgttctgc gagaagccgc tggcgcccag
      901 cctggccgag gccgaggcga tggccgacgc ggtcgcggcc gcgggggtgg tcaaccaggt
      961 cggcctgccg ctgcgacggc gtggcccctt cccggtgctg cgccacctgc tggccgaccc
     1021 ggagaacggg cccctgctgg cgatcgcgat gagcaccgtc agccgcagcc gtgaccgggt
     1081 gctggccgga tggcgtggca actccgccca ggccgggggt gggatgctga tcgaggtggg
     1141 gttccacgac atcgacctgc tgcagtggtt ggccgggccg atcggtgcgg ccaccggaca
     1201 ggtcactccc ggcacccatg ccgggatcga ggacgccgcg accgttgcgc tcggcttcga
     1261 gggcggtggc tcggcctccc tcaccgttgc gtggaacgcc gcgccggtcc cgggtcagtc
     1321 ccgccgcatc cacgtcgtgc tcgaccgggc ggagtacctg ctggaggccg acggtcagca
     1381 ccagcggctg gtggccgacg gccccggcga ccgccatcag gagtgggacg ccgacgggat
     1441 ccggggactc accaccgagc tcggcctcga gtccaacccg caggcggcct tcgtgcgcgc
     1501 cgtccgcgac ggcggaccgg cctcccccac gctcgccgat ggtgtggagg tccaccgggt
     1561 gctggaccgg gtgtaccggg cggccctggg gtgacggccc accggaccgg caccgcccgc
     1621 caccgggtcg tccccggtcc accgttcggg acgtcgcggg accggactgg tgtcgcgcgg
     1681 gggcagcagg tttcctgagc cgcgagggac gggagcacca ccatggacga acaacgacga
     1741 cggtccgcgc gctggcgcga ggactacctg cgggacggat tcctcgtcgt cgagggcgtc
     1801 ctcgacgccg ccgagctggc gacgctcggt gccgcactgg agcggatcga gcagcgggtg
     1861 gccgaggagt cgctgccgcc ccggttgcgt cagtacgtca ccctggaacg cgaccaggac
     1921 catgctggga gcgccgacac cgacaagatc tccaacatca tggagctccc gctgttcgac
     1981 ccggtgttcc gggacctgat catcaacgcc tccgtcctcg acatcctgga ggcgctgttc
     2041 gggtcgagcg agttctcctt ccacaactac aaggcgatct gcaagatgcc cgggggcgcg
     2101 gcgtccttcc gttggcaccg tgacctgccc tacctgcccc acacctcgtc gaacctgatc
     2161 acctgcatgc tctgcatcga cccgatgacg accgacaacg gcgcgaccgt ggtgtgtccc
     2221 ggctcccacc ggatcgccga tgaccggatc gatgccggcg acaaggacat cgccgaggcc
     2281 gaggtccccg cggaacgcct cacgatcgag tgcccggccg ggtcggccgt gctgttcgac
     2341 accaacatcg tccacggcgg cggacccaac cgttccgacc ggccacgccg caacgcgatc
     2401 gggatctggg cggggccgga cgtgcacccg acgaccgcgt cacgctacgc ctacgagttc
     2461 gtgatgccgc gcagcaccga tccacagcgc cagctgcagg tgcagcagac gttcggcacc
     2521 gcctgagcgg tggcgggtcg cccctcccca gacccatccc ctctcctcgt cacacccgtc
     2581 gccaccgcag ccgtgctgac gagtgagcac cgcgcagcac cgtcaccgca cgacgctagg
     2641 ctcgctcgca tgcgcttcgt cattgacgcc agttcccatc cccgccaatc gcccctcatc
     2701 cgggtcagcc ccgagcaggg acgtggcggg cagcacccca ccgcgctgcg cgacgaggcc
     2761 accggagagg tggtcgcggt ccagcacgat gagggcaccg gcgagctcag cttcgtcgtg
     2821 cccggccagc tcgccgccgg cgagacccgt accctcaccg ccaccgacga gccggtgacc
     2881 gcggcccacc cgaccactgc cgtggtgaag ctggaccggg tcgtgctgaa ggtcggcggt
     2941 gagcgtttcg ccgactacat cgtggccggc gggcgtcgtc cgtacttctg gccggtggtg
     3001 agcgcgaccg ggtcgagcgt cgtccgcggc cagggctcct cggaccaccc gcaccacacc
     3061 ggcctggcga tcagctacgg cggccatagc gaggacggtt ccaccaacat ctggtccgac
     3121 tgggacgagc ccccgtacgg accgggcggc cggatggtgc accgcggctt ccg
//
LOCUS       DATGUK010000002         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_117636,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000002 DATGUK010000000
VERSION     DATGUK010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117636"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VK103_00025"
     CDS             <1..442
                     /locus_tag="VK103_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLQ81892.1"
                     /translation="GRQNLQMYWEAAGGRPAEAGFDDALRIAGLGEATDRLVATYSTG
                     MKQRLGIAQAMLGMPAVLVLDEPINGLDPPQIRQMREVIRNYAATGRTVVVSSHLLGE
                     MEQTCSDVVVLHHGRLITAGPVTELVPEGRGLEDVFLDLIGATP"
     gene            439..1269
                     /locus_tag="VK103_00030"
     CDS             439..1269
                     /locus_tag="VK103_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003955725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLQ81893.1"
                     /translation="MSAAGTPKRHPLPLRAEVRRQLSRRRTHLAFGIVAVTPLLLVGA
                     FALAGDDDGGTGFVDLATQGALNFTLFAMFATNGFVLVVLAALFTGDTVAAEASWGSL
                     RYLLTAPVRRSRLLRQKLVVGYGSFILAMAALVAVSLLLGTISYGWSDLLTPLGVSLP
                     ADQALVRLLLAAGYIVVQLTSVAAIAFYLGTRTQAPLGAVGGSVLVVLISAILDTITA
                     LGDWRVVLPTHDQFAWTALLAEAPDWDEVLRGAGVAMIYAAVLLAAAWRHFLRADVLS
                     "
     gene            1292..>2067
                     /locus_tag="VK103_00035"
     CDS             1292..>2067
                     /locus_tag="VK103_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HLQ81894.1"
                     /translation="MHDDRVIVEDRLHRALTERLRPAVHRIVASLEIAVWHVGLQGGE
                     GREEPVAPGLALPGPTQRSDVAYRPVDIGEWWGPAWGTSWFHLTGTVPADVVGRRVEL
                     VVDLGWDGHSPGFQAEGLLYRPDGSVVKALNPQNCWAPVAEVAAGDEVIDLYVEAAAN
                     PQVLGPDGFRPTPLGERDTAGESPLYRLSRADICVHETQVWELVNDLDVLGGLMHELS
                     ESDPRRWDIVRAVERSLDRIDLDTVETTATAARAELTEVL"
BASE COUNT          303 a          689 c          738 g          337 t
ORIGIN      
        1 tggacggcag aacctgcaga tgtactggga agccgccggt ggccgaccgg ccgaggccgg
       61 attcgacgac gccctgcgca tcgcggggtt gggcgaggcg acggaccgac tggtcgccac
      121 ctacagcacc gggatgaagc agcggctcgg cattgcccag gccatgctcg ggatgccggc
      181 ggtgctggtg ctcgacgaac ccatcaacgg cctggatccc ccacagatcc ggcagatgcg
      241 ggaggtgatc cggaactacg cggcaaccgg ccgtaccgtc gtggtctcga gccacctgct
      301 gggggagatg gaacagacct gcagcgatgt cgtcgtgctg caccatggtc ggctgatcac
      361 cgcaggaccg gtcacagaac tggtaccaga aggccgcggg ctggaggatg tgttcctcga
      421 cctgatcgga gccacgccgt gagcgcagca gggacgccga aacgccaccc cctgccgctg
      481 cgggccgagg tccggcggca actctcgcgc cggcgcacgc acctcgcgtt cggcatcgtc
      541 gcggtgaccc cgttgctgct ggtcggagcg ttcgccctgg ccggtgacga cgacggtggt
      601 acaggattcg tcgacctggc cacccaaggg gcactcaact tcacgctgtt cgccatgttc
      661 gccaccaacg ggttcgtgct cgttgtgctg gctgcactgt tcaccgggga cacggtggcc
      721 gccgaggcca gctggggcag cctccggtac ctgctgacgg cgccggtgcg ccggtcgcgg
      781 ttgctgcggc agaagctggt cgtcggttac gggagcttca tcctggcgat ggccgcgctg
      841 gtggcggtgt cgctgctgtt gggcacgatc agttacgggt ggtcggacct gctcaccccg
      901 ttgggcgtgt ccctacccgc cgaccaggca ctggtgcggc tactgctggc cgccggttac
      961 atcgtggtcc aactcacgtc ggtggcagcg atcgcgttct acctgggcac ccgtacccag
     1021 gcgccgctgg gagcggtggg cggcagcgtg ctggtcgtgc tgatctcggc catcctggac
     1081 acgatcaccg ccctcgggga ctggcgagtc gtgctgccca cccatgacca gttcgcctgg
     1141 accgcgctgc tcgccgaggc acccgactgg gacgaggtgc tccgcggcgc cggggtcgcg
     1201 atgatctacg ccgcggtgct cctggcagcc gcctggcggc acttcctgcg tgcggacgtg
     1261 ctcagctaac ggcgtgtgac agtctcggcg catgcatgat gaccgcgtca tcgtcgagga
     1321 ccgacttcac cgtgccctga ccgaacgact acgtcccgct gtgcaccgca ttgtcgcctc
     1381 cttggagatc gcggtgtggc acgttggtct ccaggggggc gagggtcggg aagagccggt
     1441 tgcacccggg ctcgccctgc cggggccgac tcagcggtcc gacgtcgcct atcgccccgt
     1501 cgacatcggc gagtggtggg gaccggcctg gggtaccagc tggttccacc tgaccgggac
     1561 ggtgccggcg gatgtggtgg gacgacgcgt cgagctggtg gtcgacctcg ggtgggacgg
     1621 tcactccccg ggattccagg ccgaaggcct gctctaccgc cccgacggca gcgtcgtgaa
     1681 ggcgctcaac ccgcagaact gctgggctcc ggtagcggag gtggccgcgg gtgacgaggt
     1741 gatcgacctc tacgtcgaag ccgcggccaa cccgcaggtg ctcgggcccg acgggttccg
     1801 gcccacaccg ctcggcgagc gggacaccgc aggcgagtcg ccgttgtacc gcctttcccg
     1861 cgccgacatc tgtgtccacg agacccaggt atgggaactg gtgaacgacc tcgacgtgct
     1921 gggcgggctg atgcacgagc tgtccgagag cgaccctcgt cgttgggaca tcgtccgcgc
     1981 cgtggagcgc agcctggatc gaatagacct ggacaccgtg gagacgacgg ccaccgcagc
     2041 ccgtgccgag ctcaccgagg tgctcag
//
LOCUS       DATGUK010000003         4293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1166880,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000003 DATGUK010000000
VERSION     DATGUK010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166880"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VK103_00040"
     CDS             <1..205
                     /locus_tag="VK103_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81895.1"
                     /translation="SYQNWQMDPDGGGGRIRLAEWDPATRTVTLTVVNTASGEVEQGP
                     GHHVCVDLTPGSADIGTIHPGIA"
     gene            complement(295..1545)
                     /locus_tag="VK103_00045"
     CDS             complement(295..1545)
                     /locus_tag="VK103_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81896.1"
                     /translation="MTQQPPPFPQPGSDPTRPPDQGPPSYEAPPSYQGGASYQGGASH
                     QEQQQYGQPPGSAPYQGQEPQQGQPPHQGQPPDQGQPPLPGQPARGSGAKVAMVVGAI
                     VLAVVLLVGGGYLVVTRVLGDGPEDPTSGGPGTTGAGQGELPEQPTVEVPPVVTEVAQ
                     SAGLTCHDEATTTVKVKGCYLFEKDHLVTLRFRIADDGSINSFYVTILHNDVDPSKRA
                     AEVRSIAEPVLAELPVSDADRDAITKALAGDETDVSGDTDWSGETGGAFAYRFSKDTT
                     TLSVGEKSTDFMPRTPLAEDHQPIFDELTARGWTCETEDISITCTDGKSGKVVGNLTS
                     AGQPNNEPRLVGFRVWFNGQQPVVDEPRMVDTYEALAKAGAKGDVLAIGMRMLADGEK
                     HFFNSDVEFYRGASYYEVSGVQFI"
     gene            1856..2986
                     /gene="trpS"
                     /locus_tag="VK103_00050"
     CDS             1856..2986
                     /gene="trpS"
                     /locus_tag="VK103_00050"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HLQ81897.1"
                     /translation="MTTTSTATSTTTAPAGPADPTEQLERSRAEAQRRSALLEQQVHS
                     APGRLRLLTGDRPTGPLHLGHLFGSLRNRVRLQDAGVPTTIVIADYQVITDREPGGRE
                     RIAATAAHVRELVCDQLAAGVDPDRSTFFVHSAVPELNQLLLPFLSLVSVAELQRNPT
                     VKEEAAAAGIRSLSGLLLTYPVHQAADILGVRGTVVPVGLDQLPHLEVARTLARRFNE
                     RYGDTFTPPEALLSPAPAVLGTDGRKMSKSRGNAIQLRHTADETAAMIRSARTDSERR
                     ISYHPQSRPEVSNLLLMTSLCTGEEPEVIADRIGDRGAGELKRITIEAVNEHLAGLRG
                     RRAELAAAGDDVLQVVRDGNVRVRAVATEVLERVREAMGMRY"
     gene            3029..3562
                     /locus_tag="VK103_00055"
     CDS             3029..3562
                     /locus_tag="VK103_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HLQ81898.1"
                     /translation="MGLSRKLLGDGEEVVLHLHTHAKKVLAPALLLIVLGGLVGVALA
                     LLPPQTRPWGPVAVAGTALVVFLAFVLVPFLRWRAATYTITNRRIITRSGLLNQVGHD
                     LPLVRINDVSYERSLSDRILGCGTLNIRTASDRGPVVLPDVPDVEHVHVEITELLFES
                     TNPAYVRSSVDEEETAP"
     gene            3559..>4293
                     /locus_tag="VK103_00060"
     CDS             3559..>4293
                     /locus_tag="VK103_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLQ81899.1"
                     /translation="MTRPHPESSAPTVRLERRGAVAALLLDRPEALNAISTAHARAIG
                     EACAEVADDPTVSAVVVASTSPKAFCVGADLKERHGMSTDDLDEQRPVLSAAFRGVLA
                     LPVPTIAAVEGHALGGGFELALCCDLIVASQDAGFALPEVGLGLVPGGGGTQLLTRRV
                     GSNTAADLVFTTRRIDGTEAHRLRIVDRLTGPGGAVTAATELAERIAAQSPVALRQAK
                     IALRHGADLSIAEGLDVEERAWREAVR"
BASE COUNT          606 a         1531 c         1524 g          632 t
ORIGIN      
        1 ctcgtaccag aactggcaga tggaccctga cggcggcggt ggacggatcc ggctggccga
       61 gtgggacccc gccacccgga cggtgacgct caccgtggtc aacaccgcca gcggtgaggt
      121 cgagcagggg cccggccacc acgtgtgcgt cgacctgacg cccggctcgg ccgacatcgg
      181 taccatccac ccggggatcg cctgaccggt caggaatcga caagccgtcc gggcactcgg
      241 cccgtcgcat gacctgcatc gacacccacc caagtccggg ttccgcggtc acggtcagat
      301 gaactgcacg ccgctcacct cgtagtacga ggcaccccgg tagaactcca catcgctgtt
      361 gaagaagtgc ttctcgccgt cggcgagcat ccgcatcccg atggcgagca cgtccccctt
      421 ggcgccggcc ttcgccagtg cctcgtaggt gtccaccatg cgcggctcgt cgaccaccgg
      481 ctgctggccg ttgaaccaca cccggaaccc caccaggcgc ggctcgttgt tgggctgacc
      541 ggccgaggtg aggttgccca cgaccttgcc ggacttgccg tcggtgcagg tgatcgagat
      601 gtcctcggtc tcgcacgtcc agccgcgggc ggtgagctcg tcgaagatcg gctggtggtc
      661 ctcggcgagc ggtgtgcgcg gcatgaagtc ggtcgacttc tccccgaccg agagcgtggt
      721 ggtgtccttg ctgaaccggt aggcgaaggc cccgccggtc tcgccgctcc agtcggtgtc
      781 gcccgagacg tcggtctcgt cgccggccag ggccttggtg atcgcatcgc ggtcggcgtc
      841 cgagaccggc agttcggcca gcaccggctc ggcgatgctg cggacctcgg cggcgcgttt
      901 cgacggatcg acgtcgttgt gcaggatcgt gacgtagaag gagttgatcg acccgtcgtc
      961 ggcgatccgg aagcgcaacg tgaccagatg gtccttctcg aagaggtagc agcccttgac
     1021 cttgaccgtc gtggtcgcct cgtcatggca ggtgaggccc gccgactgcg ccacttcggt
     1081 gaccaccggc ggcacctcga cggtcggctg ctcgggcaac tcgccctggc cggcaccggt
     1141 ggtgccgggg ccgccggacg tcggatcctc cggcccgtcg ccgagcaccc gggtgaccac
     1201 caggtagccg ccaccgacca gcagcacgac ggccagcacg atcgcaccga ccaccatggc
     1261 caccttcgct ccggagccac gcgccggctg ccccggcagc gggggctgtc cctggtctgg
     1321 gggctgtccc tggtgtggtg gctgtccctg ctgcggctcc tgcccctggt acggcgccga
     1381 ccccggcggc tgcccgtact gctgctgttc ctggtgggag gcaccaccct ggtaggaagc
     1441 gccaccctgg taggaggggg gcgcttcgta cgacgggggc ccctggtcgg gtggccgcgt
     1501 cggatcggat ccgggttgcg ggaacggggg aggctgctgg gtcacactcg tccttcacat
     1561 cggtcgttgt cggtgggggg atgatcgagt ccgccggacc gagcgaggcg cgggccgacc
     1621 ggcgtcgacg cccgacgacc atggtgtcac ggcggcggct cagccaccgg gcccggctgc
     1681 tacagtcgct ggcatgtccg ggaccgtgca gcactggtgg cgtcgtacag cgacgcctcg
     1741 ccgccgcgtg tcctgacacg caccaccaca ccaccacggc cgagtcgtca cgactcggcc
     1801 gtcgtcgtcc ggtcggccgt cgtggcggac ccggccccga acaggggaga cgaccatgac
     1861 caccaccagc accgccacca gcaccaccac ggcacccgcg ggcccggccg acccgaccga
     1921 gcagctcgag cggtcccggg ccgaggcaca acgccggtcc gccctgctgg agcagcaggt
     1981 ccacagcgca ccgggccggt tgcggctgct caccggcgat cggcccaccg gcccgctgca
     2041 cctggggcac ctgttcggca gcctccgcaa ccgggtccgc ctgcaggacg ccggcgtgcc
     2101 cacgacgatc gtgatcgcgg actaccaggt gatcaccgac cgggagcccg gtggccgcga
     2161 gcggatcgcc gcgaccgctg cacacgtacg cgagctggtg tgcgaccaac tggccgccgg
     2221 cgtcgacccg gaccggtcca cgttcttcgt gcacagcgcg gtcccggagc tcaaccagct
     2281 gctgctgccg ttcctgtcac tggtcagcgt cgcggagctg cagcgcaacc cgacggtcaa
     2341 ggaggaggcg gcggcagccg ggatccgttc cctgtcgggg ttgttgctca cctatccggt
     2401 ccaccaggcc gccgacatcc tcggcgttcg gggcacggtc gtaccggtcg ggctggacca
     2461 gctgccccat ctggaggtcg ctcgcaccct cgcccggcgg ttcaacgagc gctacggtga
     2521 cacgttcacc ccgccggagg cgttgctgtc gccggccccg gccgtgctgg gcaccgacgg
     2581 tcgcaagatg agcaagagtc gcggcaacgc gatccaactg cgccacaccg ccgacgagac
     2641 cgccgcgatg atcaggtccg cccggaccga ctccgagcgt cggatcagct atcacccgca
     2701 gtcccgacca gaggtgtcca acctgctgct gatgacctcg ctgtgcaccg gagaggagcc
     2761 ggaggtgatc gccgaccgga tcggtgatcg cggagccggc gagctcaagc gcatcacgat
     2821 cgaggcggtc aacgaacacc tggccggtct gcgggggcgt cgggccgagc tggcggcggc
     2881 cggggacgac gtgctgcagg tggtgcgcga cggcaacgtc cgggtccggg cggtggccac
     2941 cgaggtgctg gagcgggtgc gcgaggcgat ggggatgcgc tactgacgac ccgggtgcgc
     3001 gggcgcgcgg ttgtgcaagc atggatgcat gggtctgtcc cgcaagttgc tcggtgacgg
     3061 cgaggaggtc gtgctgcacc tgcacaccca cgccaagaag gtgctcgccc cggcactgct
     3121 gctcatcgtg ctgggcgggc tggtcggtgt cgcgctggcc ctgctgccgc cgcagacccg
     3181 cccgtggggg ccggtggccg ttgccgggac cgccctggtg gtgttcctgg cgttcgtgct
     3241 ggtgccgttc ctgcggtggc gcgccgccac ctacaccatc accaaccgcc gcatcatcac
     3301 tcggtcggga ctgctcaacc aggtcgggca cgacctgccg ctggtccgga tcaacgacgt
     3361 gtcgtacgag cgcagcctca gcgaccggat cctggggtgc ggcacgttga acatccgcac
     3421 cgcgtccgac cgcggacctg tcgtgctgcc cgacgtgccg gatgtcgaac acgtccacgt
     3481 ggagatcacc gagctgctgt tcgagtccac caacccggcc tacgtacgca gctccgtcga
     3541 cgaggaggag accgcaccat gacccgacca cacccagagt cgtccgcgcc gacggtccgg
     3601 ctggagcggc gcggcgccgt cgcggccctg ctgctggacc gtcccgaggc actcaacgcc
     3661 atctcgaccg cccatgcccg ggcgatcggc gaggcctgtg cggaggtcgc cgacgacccc
     3721 actgtcagcg cggtggtggt ggccagcacc agcccgaagg cgttctgtgt cggtgccgac
     3781 ctgaaggaac gtcacggcat gtccaccgac gacctggacg agcagcggcc ggtgctgtcg
     3841 gccgccttcc gtggggtgct ggcgctgccg gtgccgacga tcgccgccgt ggagggccac
     3901 gcactgggcg gtggcttcga actggcgctg tgttgcgact tgatcgtcgc ctcgcaggac
     3961 gccgggttcg cgctgccgga ggtcgggctc ggcctggtgc ccggcggtgg gggcacccag
     4021 ctgctcactc gccgggtcgg gtcgaacacc gccgcggacc tggtgttcac cacccgccgg
     4081 atcgacggca ccgaggcaca ccggttgcgg atcgtggacc ggctcaccgg tccgggcggg
     4141 gcggtcacgg cggccaccga gctggccgaa cggatcgccg cgcagtcgcc ggtggcgctg
     4201 cgacaggcca agatcgcgct ccgccacggc gccgacctgt cgatcgccga gggcctcgac
     4261 gtggaggagc gggcgtggcg ggaggccgtc cgc
//
LOCUS       DATGUK010000004         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1399432,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000004 DATGUK010000000
VERSION     DATGUK010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399432"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="VK103_00065"
     CDS             complement(<1..1454)
                     /locus_tag="VK103_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLQ81900.1"
                     /translation="MDRALTRRTLLGTGLAAAAAGGLAACGGGKESEERTTENRNVVL
                     PTYQQVELVQPDLPGNEVLMPGYYSYPRDPKKVFDTPPGAPLDELSMMYTTFIPAPKG
                     PESNSFWAQLQEDIGTTLKLQPVSSGDYDKKFQTLIAGGDMPDIMNFPLPTPNQPQMM
                     EKVFADLGPYLSGDAAKEFPYLANIPEVSWPVGIANGSIYAVPQPRAITGQVLYYRKD
                     LFDEYGITHEPADYDEFIGTFEAYTDRDKDRFAFSNIGQMHYRILGMMGGPNKWSVSD
                     DGTFTPQYLDPIYRESLAMMPELVKKYAHPESHTAPYAQHREFFYHGNCCYLHDGYAG
                     WDLYVRGLQGAVDKLGMMVQPKFDGGGDGPQFTSKSYQGITVINKKLTGEKLRAAVDV
                     LNFLCAPIGSLEHLHRKFGKEGKDWEWKGDTVELTEEGQRAFMDFQYIADAPTVLGPG
                     AQEEVQPQHAYMERMSKVLVDDPSMGLYSDTHSR"
     gene            complement(1531..>2344)
                     /locus_tag="VK103_00070"
     CDS             complement(1531..>2344)
                     /locus_tag="VK103_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLQ81901.1"
                     /translation="WRGGTHGDFWHYRRIVDVTRYPEGRYASDVTLVNWPQIDYMAGP
                     LLGVDAATREHHLAESRQQSLSFLYWLQTECPRHDSDGTGYPELRPRGDLLGSEDELA
                     LRAYIRESRRIKAEFTVVEDHVGVEARQAAGLPAGSALFADTIGVGSYRIDLHPSTGD
                     ETGPRNYIDVSNYPFQVPLGSLIPQRVDNLLAGAKNLGVTHITNGCYRLHPVEWVIGE
                     AAGRLAAHCLDIAESPRAVRNTPERLSEFQARLSSDGGFRLAWPDRIRRTER"
BASE COUNT          345 a          755 c          809 g          435 t
ORIGIN      
        1 ttgcggctgt gggtgtcgga gtacaggccc atcgaggggt cgtccaccag caccttcgac
       61 atccgctcca tgtaggcgtg ctggggctgc acctcctcct gcgcaccggg accgagcacg
      121 gtgggcgcat cggcgatgta ctggaagtcc atgaaggcgc gttgcccctc ctcggtgagc
      181 tcgacggtgt cgcccttcca ctcccagtcc ttgccctcct tgccgaactt gcggtgcagg
      241 tgctccagcg acccgatcgg cgcgcagagg aagttgagca cgtcgacggc ggcgcgcagc
      301 ttctcgccgg tcagcttctt gttgatcacc gtgatgccct ggtagctctt cgaggtgaac
      361 tgcggaccgt ccccgccgcc gtcgaacttc ggctgcacca tcatcccgag cttgtcgacc
      421 gcaccctgca ggccgcggac gtacaggtcc catccggcgt agccgtcatg caggtagcag
      481 cagttgccgt ggtagaagaa ctcccggtgc tgggcgtacg gtgcggtgtg cgactccggg
      541 tgggcgtact tcttgaccag ctccggcatc atcgccaacg actcgcggta gatcgggtcg
      601 aggtactgcg gggtgaacgt cccgtcgtcg gacacgctcc acttgttggg gccgcccatc
      661 atccccagga tgcggtagtg catctggccg atgttggaga aggcgaaccg gtccttgtcg
      721 cgatcggtgt aggcctcgaa ggtcccgatg aactcgtcgt agtcggccgg ctcgtgggtg
      781 atgccgtact cgtcgaacag atccttgcgg tagtacagca cctgtccggt gatggcgcgc
      841 ggctgcggga ccgcatagat ggagccattc gcgatgccga ccggccacga cacctccggg
      901 atgttcgcca ggtacgggaa ctccttggcc gcatcgccgg acaggtacgg cccgaggtcg
      961 gcgaagacct tctccatcat ctgcggctgg ttcggcgtgg gcagcgggaa gttcatgatg
     1021 tccggcatgt caccaccggc gatcagcgtc tggaacttct tgtcgtagtc ccccgacgag
     1081 accggctgca gtttcagcgt ggtgccgatg tcctcctgca gctgcgccca gaagctgttc
     1141 gactcggggc ccttcggcgc cgggatgaac gtggtgtaca tcatgctgag ctcgtccagc
     1201 ggagcccccg gaggagtgtc gaagaccttc ttcggatcgc gcgggtacga gtagtagccc
     1261 ggcatcagca cctcgttgcc cggcaggtca ggctgcacca actccacctg ttggtaggtc
     1321 ggcagcacga cgttgcgatt ctcggtcgtc cgttcctcgg actccttgcc gccaccgcag
     1381 gcggcgaggc caccggcggc agcggccgcg agtcccgtcc cgaggagggt gcggcgcgtc
     1441 agggctcggt ccatgggtca ctccacttca ttgtcagtgc tggttcgttg tcagtgctgg
     1501 attgtgtgca gggtcgggcc catcaggggg tcaccgctcg gtgcggcgga tccggtccgg
     1561 ccaggcgagc cggaagccgc cgtcgctgct gagtcgggcc tggaactcgc tgagtcgctc
     1621 cggggtgttg cggaccgcac gcggcgactc ggcgatgtcg aggcagtggg cggcgagtcg
     1681 gccagcggcc tccccgatca cccactccac cgggtgcagt cggtagcaac cgttggtgat
     1741 gtgggtgacg ccgaggttct tcgcgccggc cagcaggttg tccacccgct gggggatcag
     1801 cgagcccagt ggcacctgga acgggtagtt cgacacgtcg atgtaattgc gcggaccggt
     1861 ctcgtcaccc gtgctggggt gcaggtcgat ccggtacgag ccgacgccga tggtgtcggc
     1921 gaacagcgcc gatccggccg gcagaccagc agcctgacgt gcttccacgc cgacatggtc
     1981 ctccaccacg gtgaactccg ccttgatgcg acgtgactcc cggatgtacg cccggagtgc
     2041 gagctcgtcc tccgacccga gcaggtcgcc acggggccgc agttccgggt agccggtgcc
     2101 gtcactgtcg tggcgtgggc actcggtctg caaccagtac aggaaggaca ggctctgctg
     2161 gcgtgactcg gcgaggtggt gctcccgggt cgcggcgtcg acgcccagca gcggtccggc
     2221 catgtagtcg atctgcggcc agttcaccag ggtgacgtcg ctggcgtagc ggccctccgg
     2281 gtaccgggtg acgtcgacga tccggcggta gtgccagaag tcgccgtggg tgccaccgcg
     2341 ccag
//
LOCUS       DATGUK010000005         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_471742,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000005 DATGUK010000000
VERSION     DATGUK010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471742"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..1195
                     /locus_tag="VK103_00075"
     CDS             314..1195
                     /locus_tag="VK103_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLQ81902.1"
                     /translation="MRAESVPPATTQEHDPTTGSVRAPHDRAERQSLARWTRSQTIRA
                     GDADPEERRSIHGSVTLAHLWLADSVARRFIGKGEDLDDLQQVARAALVEAVGRYDPD
                     QGDFIPFAVPTIAGLLKRHFRDHAWTIRPPRRTQEIAVTIRREWPTLAQSLGHQPTTR
                     DLADHLDCDETDIRRAQVADRGYGSESLDDDSAGQGRRLQDGEPARSSDAALVVRRAC
                     AGLDREDRWLLYLRFAEERTQSDIAQRLGTSQMQVSRRLARLLRRMRSSIGPLDAPSS
                     RARRSASARTHATAPAG"
     gene            1200..2042
                     /locus_tag="VK103_00080"
     CDS             1200..2042
                     /locus_tag="VK103_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HLQ81903.1"
                     /translation="MIHETQHHRRPRTCGCAVDGADAAARTDGAGPTSVDRVLRATGD
                     FAQDLSHHTGVDEVLATCVSLLPRVVPGADHITLTSSAGAAGSDDAAVAATTDPTAAV
                     TLLLQYELREGLAIDALRSGLPIVSNDLQQESRWPRWLPRLTSTTALRSMLTITVCPG
                     RSPTYVLTLASDRCDVYRQVSVQAAEAAAAHLTVAVETASTIHHRGVAMETRTAIGRA
                     EGMVMERLGLSADQAHCYLQRVSQQSNRKLAELADELVRTGLLPDASARGDLGQYPTD
                     RSHG"
     gene            complement(1992..>2431)
                     /locus_tag="VK103_00085"
     CDS             complement(1992..>2431)
                     /locus_tag="VK103_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLQ81904.1"
                     /translation="PGMEIETVPTIAELMTPTPAWVGASWPLIDAARRMRDLEVGTLP
                     ILGPGQRLVGMLSDRDIVVRCVAAGADPAVVHAGDLARGTPAVVQATAPAETALHLMA
                     RHRVRRLPVVDGDALVGIVAQADVARALPVAAVGRVLAEVSTS"
BASE COUNT          379 a          893 c          837 g          322 t
ORIGIN      
        1 ggccgcccca ccgaccagca acatccgccg gcccggtccc ggagccgggc acggtggtgg
       61 tcgagcgtct cggtacgaca cgtcgacctc ctcgactcgg tccggtccgg catcgaccca
      121 ctgctcccgg tcgtaccccg gcccggactc gggaaaccgg tcaggacccg gtccgttccg
      181 caccgatccg tatgcacacg gtggccgccg gcgaggctgc gcccgacccc ttgacgaagc
      241 tcgggatccg tgctgcgctg agggttcgtg gtgcaaacag ccgccacccc actacggact
      301 ggaaggctgt gccatgcgcg ccgagtctgt accccctgca acaacacaag agcacgaccc
      361 gaccaccggt tcggtccggg ccccgcacga tcgcgcggaa cgccagagcc tggcccggtg
      421 gacccggagc cagacgatcc gggccggtga cgccgacccg gaggagcgtc gctcgatcca
      481 cggcagcgtc acgctggccc acctgtggct cgccgactcc gtggcacggc gattcatcgg
      541 aaagggtgag gacttggatg acctgcagca ggtcgcccgg gccgcactcg tcgaggccgt
      601 cggccggtac gatccggacc agggcgactt catcccgttc gccgtcccga ccatcgcggg
      661 gctgctcaag cgacacttcc gcgaccatgc ctggacgatc cgcccgccgc ggcgaaccca
      721 ggagatcgcg gtgacgatca ggcgggagtg gccgacactg gcccagtcgc tcggacacca
      781 accgaccacg cgtgacctgg cggaccacct ggactgcgac gagaccgaca tccgacgcgc
      841 ccaggtcgcc gaccggggct acggcagtga gtccctcgac gacgacagtg ccgggcaggg
      901 gcgccgcctg caggacggcg aaccggcccg ctcgtcggat gccgcgctgg tggtccgacg
      961 tgcctgtgcc gggctcgacc gggaggaccg ctggttgctg tatctccggt tcgccgagga
     1021 acgcacccag agcgacatcg cccagcggct gggcaccagc cagatgcagg tgtcgcggcg
     1081 gctggccagg ctgctgcgcc ggatgcgatc ctcgatcggc ccactggacg cccccagcag
     1141 ccgggctcgc cgatccgcgt cggcccgcac ccacgccacg gctccggcag ggtgatgggg
     1201 tgatccacga gacgcagcac caccgtcgac cgcgcacctg cgggtgcgcg gtcgatggag
     1261 ccgatgcggc cgccaggacc gacggggccg gtcccacgtc ggtcgaccgg gtgctgcggg
     1321 ccaccgggga ctttgcccag gacctgagcc accacaccgg ggtggacgag gtgctggcga
     1381 cctgcgtgtc actactgccc cgggtggtgc ccggggccga tcacatcaca ctcacatcga
     1441 gcgccggtgc ggcaggatcc gacgatgccg cggtcgcggc gacgacggat ccgaccgccg
     1501 cggtcacact gctgttgcag tacgagctgc gggagggcct cgcgatcgat gccctccggt
     1561 ccgggctgcc gatcgtctcg aacgacctgc agcaggagag ccgttggccg agatggctgc
     1621 cgcggctcac ctcgacgacc gcgctgcgtt cgatgttgac gatcacggtg tgcccgggac
     1681 ggtccccgac ctatgtgttg accttggcat ccgaccggtg cgacgtgtac cggcaggtga
     1741 gcgtgcaggc ggccgaggcg gccgccgccc acctcaccgt cgccgtcgag actgcgtcca
     1801 ccatccacca ccgcggtgtg gcgatggaga cccggacggc gatcgggcgc gcggagggca
     1861 tggtgatgga acggttggga ctgtcggcag accaggcgca ctgctacctg cagcgggtgt
     1921 cgcaacagag caaccgcaaa ctggccgagt tggccgacga attggtccgc accggacttc
     1981 tgcccgatgc ctcagctcgt ggagacctcg gccagtaccc gaccgaccgc agccacgggt
     2041 aacgcacggg ccacatcggc ctgcgcgacg atgccgacca gggcgtcacc gtccaccacc
     2101 ggcagccgtc gcactcgatg acgcgccatc aggtgcaatg ctgtctcggc aggcgcggtg
     2161 gcctgcacca cggcgggcgt cccccgcgcc aggtcaccgg cgtgcaccac cgccgggtcg
     2221 gcgcccgccg cgacacagcg caccacgatg tcgcgatcgc tcagcatgcc gacgagtcgc
     2281 tgaccgggtc ccaggatggg cagggtgccc acctccaggt cgcgcatcct acgggcggcg
     2341 tcgatcagcg gccaggacgc ccccacccat gcgggggtcg gggtcatcag ctctgcgatt
     2401 gtcggcacgg tctcgatctc catccccggc g
//
LOCUS       DATGUK010000006         4652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1169187,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000006 DATGUK010000000
VERSION     DATGUK010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169187"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VK103_00090"
     CDS             complement(<1..322)
                     /locus_tag="VK103_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018552398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLQ81905.1"
                     /translation="MPVTRAVPVREDGAVSIEPARRADGPRGRIESPNRLSLGRRIWR
                     DRAMLLLAIPGLMYFVVFHYVPLLGYVVAFQNYKPFLGFRKSPFVGFDNFTYIVSDVA
                     FWQAL"
     gene            464..2413
                     /locus_tag="VK103_00095"
     CDS             464..2413
                     /locus_tag="VK103_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81906.1"
                     /translation="MNHTLTDLGPANTSMFPLMSALQLGTLLHIGSRNLTPSMAITYD
                     VAARRVVARHDFGPGKFVQALAARPGDTGDGATIYAGLVGCDDHEPNIHAFPSTPAGP
                     TDGDSVDTTPGRPVATIPGVGVRVLACAPDGTLYAAGKEDAPAVWQVTDGTEVRPRRL
                     AVPDPTTTQCRGLAADEEYVYFGCGSNLAGGHGAGLTRVFRVHRDTGATEDLTPDELR
                     ADPAVRQLHRVGRRLLVGTQGLGRRSHLAVIDLDTGRTLRVWELPGKSVGCFAAGPDG
                     TVLLAAATLHRVDPERLELSDGPTTSAGERWGLGHHGDEVLTVSAMGQVVHSSDRGDE
                     RVRLVEVGAPALPQLGMSLQVTDGRLYVGGNGGVVVHELATAPWGPDMRDKRLEVPGE
                     AKTITCVDDVAHLAVYSSQGLWTHDPGTGALGRAVEVPAEFNRPQDSCWDDEGRRLLV
                     GYQNDTQGGGALVVWTPGSGDLLVHTNPFDEYQMVRAVTSRDGDAWLGGWNRYEQGPR
                     GTVARVDPGTAAIRWRQDGAGAGISCLGLHGPYLWAITMTGEALAFDARSGEPCGRQD
                     LSHQLEGMTRIVETPAGVVVGSADTVLRLSSPDRGPELIVTGLDGQWYSGPKIAQDSA
                     GRLYTLRGRHVVRIAAPHAVDGRRD"
     gene            2406..3299
                     /locus_tag="VK103_00100"
     CDS             2406..3299
                     /locus_tag="VK103_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLQ81907.1"
                     /translation="MTDRRLRLGIDLGGTKVAVGGVDTAGALAGALSLVHDGTLPGVL
                     TTVGRALSQLREEGIGHPFVSAGVGVPGAVDDEGRISLCPALPDLESVDLSAALSDLL
                     GVAVEVENDSNAAALGEYRYGNHGVEDLACVCLGTGIGLGLVSDGRLLRGARGLAAEI
                     ADLSVDDGTGQQVRTEDVAHAAALARAAGHADKAAMPQVLVEHHRGSAAAIGAVGRYA
                     AVVANAVVATAVLVDPAVVLLSGGLGSQPAIADQVTRRIRAQRPGLPVRIARYGARSP
                     LVGAASLTATGRDTAPSVRRA"
     gene            3305..>4652
                     /locus_tag="VK103_00105"
     CDS             3305..>4652
                     /locus_tag="VK103_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81908.1"
                     /translation="MATSAVTGFPLMSSCLVGDVLYVGSRNLRPTRVAAIDLTTRRTV
                     AVHDLPTGNFVQTLRAVDAPAHGGDSPAHGGDTVYAGVTHAPSGPNVHRIDGATGEVV
                     PWASVPDMYVRDLCPLPDGRVAVVGRQPRRGRGPGVWLAGPDTATEVCVPDPAATQGL
                     CIDTVGDQLWVGTGANLGGDGSGSARLFAIDPDGTVHPRAARRLTGSRAVRCVASDGT
                     VVVAGTEAATAVVVEVDLRTGAERVWPLHAKVVSRVLVLPDRHLVTTAEPCQLWQVDR
                     STGQTRLLGVPLPDGEVWGLHVHAGTADRPTGPDVVGSASGGWLFEVDVGSCAAEPPQ
                     DRTRRTRVTELGVPPGAQLGMSIAAVPERYAAGPAVVTAGNFTLQRHDLTTGEVTEVA
                     LPGEAKDMARLGDTLHLGVYNSQGLWSLDLTDPEARPRRTVALPVHLNRPQVVAADD"
BASE COUNT          647 a         1699 c         1700 g          606 t
ORIGIN      
        1 ccaacgcctg ccagaaggcc acatcgctga cgatgtaggt gaagttgtcg aagcccacga
       61 agggcgactt ccggaagccc aggaacggct tgtagttctg gaaggccacc acgtagccca
      121 gcagcggcac gtagtggaac accacgaagt acatcagccc ggggatcgcc agcagcaaca
      181 tcgcccggtc ccgccagatc cgtcgcccca gcgacagccg gttcggcgac tcgatccgac
      241 cccgcggccc gtcggcccgc cgagccggct cgatcgacac ggccccgtcc tcgcggaccg
      301 gcaccgcccg ggtgactggc atcgtcacga cccccttgac gtcgacttac gcatcagtat
      361 gatcgttaag tgtcgaagac agtcttaacc gtcccgacat ggactgtcaa tcaatcgatc
      421 gcatctggac caactttgtg atcgttacga gggagaagcg cgcgtgaacc acacgttgac
      481 cgacctgggc ccggcgaaca cgtcgatgtt cccgctgatg agtgcgctgc agctcggcac
      541 cctgctccac atcgggtccc gcaacctcac cccgtcgatg gcgatcacct acgacgtcgc
      601 cgcacgccgg gtggtggccc ggcacgactt cggtccgggc aagttcgtgc aggcgctggc
      661 ggcccggccc ggcgacaccg gcgacggcgc gacgatctac gccgggctgg tcggctgcga
      721 cgaccacgaa cccaacatcc acgccttccc gagcaccccc gccggaccga ccgacggcga
      781 cagcgtcgac accacccccg gccgcccggt cgccacgatc cccggagtcg gcgtgcgggt
      841 gctcgcctgt gctcccgacg gcacgctgta cgccgcgggc aaggaggacg ccccggcagt
      901 gtggcaggtc actgacggca ccgaggtccg gccgcgtcgg ctcgccgtac ccgatcccac
      961 caccacgcag tgccgtggcc tggccgccga cgaggagtac gtctacttcg ggtgcggcag
     1021 caacctggcc ggcggtcacg gtgccggact gaccagggtg ttccgggtgc accgcgacac
     1081 cggcgcgacc gaggacctga cgccggacga gctgcgggcc gatccggccg tgcgtcagct
     1141 gcaccgcgtc gggcgtcgcc tgctggtggg cacccagggc ctcgggcgcc gcagccatct
     1201 ggcggtcatc gacctcgaca ctgggcggac cctgcgggtg tgggagctgc ccggcaagtc
     1261 cgtcggctgc ttcgccgccg gaccggacgg caccgtgctg ctcgccgcgg caaccctgca
     1321 ccgggtggac ccggagcgac tcgaactgtc cgacggcccc accaccagtg ccggggaacg
     1381 gtgggggctc ggccaccacg gcgacgaggt gctcaccgtc tccgcgatgg gccaggtggt
     1441 gcacagcagc gaccgcggtg acgaacgggt ccggctcgtg gaggtcggcg ccccggccct
     1501 gccacaactg gggatgtcgc tgcaggtgac cgacggccgc ctctacgtcg gcggcaacgg
     1561 cggtgtcgtc gtgcacgagc tcgccaccgc cccgtggggc cccgacatgc gcgacaagcg
     1621 actggaggtg cccggcgagg ccaagacgat cacctgcgtc gacgacgtcg cccacctcgc
     1681 cgtctacagc tcccagggac tctggaccca cgacccggga acgggtgcgc tgggccgggc
     1741 ggtggaggtg ccggcggagt tcaaccgccc ccaggacagt tgctgggacg acgagggacg
     1801 acgcctgctg gtcggctacc agaacgacac ccagggcggc ggcgccctgg tggtgtggac
     1861 accgggcagc ggcgacctgc tggtgcacac caacccgttc gacgagtacc agatggtgcg
     1921 ggcggtcacc agccgcgacg gtgacgcctg gctcggcggg tggaaccggt acgagcaggg
     1981 ccctcgcggc acggtggcac gggtcgaccc ggggaccgcc gcgatccggt ggcgccagga
     2041 cggcgccggg gccggcatca gctgcctcgg tctgcacggc ccgtacctgt gggcgatcac
     2101 gatgaccggc gaagccctgg cgttcgacgc ccggagcggg gagccgtgcg gacgccagga
     2161 cctgtcgcac cagctggagg gcatgacccg gatcgtggag accccggccg gggtggtggt
     2221 cggctcggcc gacacggtgc tgcggctgtc gtcgcccgat cgcggacccg aactgatcgt
     2281 caccggcctc gacggccagt ggtacagcgg accgaagatc gcccaggact ccgcgggccg
     2341 gctgtacacc ctgcgtggcc gccacgtcgt gcgcatcgcc gcaccgcacg ccgtggacgg
     2401 tcgacgtgac tgaccggcga ctgcgcctgg ggatcgacct gggcggcacc aaggtggcgg
     2461 tcggaggtgt cgacaccgcc ggcgcactcg ccggcgcgct ctccctggtc cacgacggca
     2521 cgctgcccgg cgtgctgacg accgtagggc gtgccctgtc ccagctgcgt gaggagggga
     2581 tcggccaccc gttcgtctcg gccggtgtcg gggtgcccgg tgcggtcgac gacgagggtc
     2641 ggatcagcct gtgcccggcg ctgcccgacc tggagtcggt cgacctgtcc gccgccctca
     2701 gcgacctgct cggcgtggcg gtggaggtgg agaacgacag caacgccgca gcgctcgggg
     2761 agtaccgcta cggcaaccac ggtgtcgagg acctggcgtg tgtgtgcctg gggaccggga
     2821 tcgggctcgg cctggtcagc gacgggcggc tgctgcgcgg ggcccgcggc ctggccgccg
     2881 agatcgccga cctgtccgtc gacgacggca ccgggcagca ggtccgcacc gaggacgtgg
     2941 cccacgccgc tgcactcgcc cgtgctgctg gccacgccga caaggccgcg atgccacagg
     3001 tgctcgtcga gcaccaccgt ggctccgccg ccgcgatcgg cgctgtgggc cggtatgcgg
     3061 cggtggtggc caacgcggtc gtcgcgaccg ccgtgctggt ggacccggcg gtggtgctgc
     3121 tgtcgggcgg gctcggcagc cagccggcga tcgccgacca ggtgacccgg cggatccggg
     3181 cccagcgacc ggggctgccg gtccggatcg cccggtacgg ggcgcgctcc ccgctggtcg
     3241 gggccgcctc cctcaccgcc actggccggg acaccgcacc gtcggttcgc cgggcgtgag
     3301 gacgatggcc acctcggccg tgaccggctt cccgctgatg tcgagctgtc tggtcggcga
     3361 cgtgctgtac gtgggttcgc gcaacctgcg cccgacccgg gtggccgcga tcgacctcac
     3421 cacccggcgc accgtggccg tccacgacct gccgaccggc aacttcgtcc agacactgcg
     3481 ggccgtcgac gcccctgccc acggcggtga ctcccctgcc cacggcggtg acaccgttta
     3541 cgccggggtc acccacgcac cgtccgggcc gaacgtgcac cggatcgacg gagccaccgg
     3601 cgaggtggtg ccgtgggcgt cggtgccgga catgtacgtc cgcgacctgt gcccgctgcc
     3661 cgacgggcgg gtcgcggtgg tgggccggca accccggcgt ggccgcgggc ccggcgtgtg
     3721 gctggccggc cccgacaccg cgaccgaggt gtgtgtcccc gacccggcgg ccacccaggg
     3781 gttgtgcatc gacacggtgg gcgaccagct ctgggtcggc accggcgcca atctcggcgg
     3841 cgacggcagc ggcagcgcca ggctgttcgc gatcgacccc gacggtacgg tgcacccgcg
     3901 cgcggcccgc agattgaccg ggagccgggc cgtacgctgc gtggcctccg acgggaccgt
     3961 tgtggtggcc gggaccgagg cggccaccgc cgtggtcgtc gaggtcgacc tccggaccgg
     4021 ggccgagcgg gtctggcccc tccacgccaa ggtggtctcc cgggtcctgg tgctgcccga
     4081 ccggcacctg gtcaccacgg ccgagccgtg ccagctgtgg caggtcgacc ggtccaccgg
     4141 acagacgcgc ctgctcggcg tgcccctgcc cgacggcgag gtctggggcc tgcacgtgca
     4201 cgcggggacg gcggaccgac ccaccggtcc cgacgtggtc gggtccgctt ccgggggctg
     4261 gctcttcgaa gtcgatgtgg ggtcgtgtgc cgcggagccc ccgcaggacc gcaccaggcg
     4321 gacccgggtg accgagctgg gggtgccgcc cggcgcccaa ctggggatgt cgatcgccgc
     4381 ggtccccgag cggtacgcgg ccggcccggc cgtggtgact gcgggcaact tcaccctgca
     4441 gcgccacgac ctgaccaccg gtgaggtcac cgaggtcgcg ctgcccggtg aggccaagga
     4501 catggcacgc ctcggcgaca cgctgcacct cggggtctac aactcgcagg gactgtggag
     4561 cctggacctg accgacccgg aggcgcggcc gcggcgcacg gtggcactgc cggtgcacct
     4621 gaaccgtccc caggtggtgg ccgccgacga cg
//
LOCUS       DATGUK010000007         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_476834,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000007 DATGUK010000000
VERSION     DATGUK010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476834"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1334
                     /locus_tag="VK103_00110"
     CDS             111..1334
                     /locus_tag="VK103_00110"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HLQ81909.1"
                     /translation="MAMVATAAGPTLPSVAADDDPPPLGIGLPDLPETRHTTEVAPGI
                     THTTIERGLPLEDPDEIPTTQTGPWVVNVLTIDPDVADGRMAVTDGPTLADTETVQDL
                     AAYSGALAAINSSFFTFTGDPDYPGDAGGLTIRGGRVVSEPSLTQEREVDLIFDSRTN
                     RMRITSTTWRGTVTMDSGTTLTLDRLNHRPVVPQECTDLADQTECDVPGELVRFHDDF
                     AAETPPGHGVEVVFDDNNCVVAVHQERGTALRPGQTSVQATGSDAAELAALGEGGGCG
                     TAQDRVYDDQGRRIPLTPHTWAVTGRVPLVADGHSVVPDSDDSFFGRHPRTFVGKTAD
                     GSWMFVVVDGRALTSVGATLDECARIALDLGMVDAINLDGGGSTTMVIGGELINRPST
                     DGKQRKVGDALVWLP"
     gene            1403..1921
                     /locus_tag="VK103_00115"
     CDS             1403..1921
                     /locus_tag="VK103_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81910.1"
                     /translation="MDDTSHAVVDVVARLCRTTLALGVVVAAAGGLVAPIVSGALSEE
                     GDPVALGLLEWSALELESVSTGARLWGIVVTSLCWLSLAVAAVAVVRWFRVLPRVTSV
                     VGTVVTAAHLLCAVLSYMVGGQVADRAGDDWQYQWGWWVPVLAGVLSGLTIWFDDSLR
                     HAGWRDLTLPRH"
     gene            1949..>2150
                     /locus_tag="VK103_00120"
     CDS             1949..>2150
                     /locus_tag="VK103_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HLQ81911.1"
                     /translation="MTPKKQSGSATPATRVLDRAGVRYTLHPYQHNPAAVHFGEEAAQ
                     RLGVDPATLLKTLVVDTGAALAV"
BASE COUNT          297 a          791 c          750 g          312 t
ORIGIN      
        1 gtcgtgaaat ctgtttgcac atgactgttc ccatcattcg gagcgccgat gctcccaccg
       61 gccgtcgatc cctgcgacgg cgtactgcca gcctgctggt cccgctcgcc atggccatgg
      121 tggccaccgc ggcagggccg acgctgccca gcgtcgcggc ggacgacgac ccgcccccgc
      181 tcggcatcgg gttgcccgac ctgccggaga cccggcacac cacggaggtc gccccgggca
      241 tcacccacac cacgatcgag cgcgggctgc cgctggagga ccccgacgag atcccgacca
      301 cccagaccgg accgtgggtg gtgaacgtgc tcaccatcga cccggacgtc gccgacggcc
      361 ggatggcggt gactgacggg ccgacgctcg ccgacaccga gaccgtccag gatctcgccg
      421 cgtacagcgg ggcgctggcc gcgatcaact ccagcttctt caccttcacc ggcgacccgg
      481 actaccccgg cgatgccggt ggtctgacga tccgcggcgg acgggtcgtc tccgagccca
      541 gcctcaccca ggaacgcgag gtggacctga tcttcgactc gcgcacgaac cggatgcgga
      601 tcacctccac cacttggcgg ggcacggtca cgatggactc cggtacgacg ctcaccctgg
      661 accggttgaa ccatcgcccc gtggtgcccc aggagtgcac cgacctggcc gaccagaccg
      721 agtgcgacgt gcccggggaa ctggtccgct tccacgacga cttcgccgcc gagaccccac
      781 ccggccacgg ggtcgaggtg gtcttcgacg acaacaactg cgtggtggcg gtccaccagg
      841 agcgcggtac ggcgttgcgt cccggccaga cctcggtgca ggccaccggc tccgacgccg
      901 ccgagttggc cgccctcggt gagggcggcg gatgtggcac cgcccaggac cgcgtctacg
      961 acgaccaggg gcgccggatc ccgctcaccc cgcacacttg ggccgtcacc ggccgtgtcc
     1021 cactggtggc cgacggccac agcgtcgtgc ccgactccga cgacagcttc ttcgggcggc
     1081 atccgcggac cttcgtcggc aagaccgccg acggcagctg gatgttcgtg gtggtcgacg
     1141 ggcgtgcgct caccagcgtc ggcgccaccc tggacgagtg cgcccggatc gccctggacc
     1201 tgggcatggt cgatgccatc aacctcgacg gcggtgggtc gaccacgatg gtgatcggcg
     1261 gtgagctgat caaccgaccc agcaccgacg gcaagcaacg caaggtcggc gacgccctgg
     1321 tgtggctccc ctgagcggtc gggtccggcc gcggcctcag ggccgcggcc gccaggcccc
     1381 ggacggtgtt agcgtcccgg ccatggacga cacctcacac gccgtggtcg acgtggtcgc
     1441 ccggctctgt cgcaccaccc tggcgctcgg ggtcgtggtc gccgccgctg gcgggctggt
     1501 cgcgcccatc gtgtcgggtg cgctcagtga ggagggcgac cccgtcgcac tcggcctgct
     1561 ggagtggtcc gccctggagc tcgagtcggt ctccaccggg gcccggctgt ggggcatcgt
     1621 ggtcacgtcg ctgtgctggc tgagcctggc cgtcgcggcg gtggccgtcg tccggtggtt
     1681 ccgcgtgctg ccacgggtga cgtcggtcgt cggcacagtg gtcacggcgg cacatctgct
     1741 gtgtgccgtg ctgagctaca tggtcggcgg tcaggtcgcc gatcgtgccg gcgacgactg
     1801 gcagtaccag tggggctggt gggtgccggt cctcgccggt gtgctgagcg gactgacgat
     1861 ctggttcgac gacagcctgc ggcacgcggg ctggcgggac ctcaccctcc cccggcactg
     1921 acctgcccgg gcgtgccaga gtggcccggt gacaccgaag aagcagtcgg gatcggccac
     1981 cccggccacc cgtgtcctcg accgggccgg ggtccggtac accctgcacc cgtaccagca
     2041 caacccggcc gcggtgcact tcggcgagga ggccgcccaa cggctcgggg tggacccggc
     2101 caccctcctc aagacgctgg tggtcgacac cggcgccgcc ctggcggtcg
//
LOCUS       DATGUK010000008         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_825235,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000008 DATGUK010000000
VERSION     DATGUK010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825235"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VK103_00125"
     CDS             complement(<1..283)
                     /locus_tag="VK103_00125"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HLQ81912.1"
                     /translation="MQSIDIDIPVELCDDIAHTDTGAQVFVLALEAAQVDDGSPLALE
                     GSARYMWRQLTAGPTSVAELAARAAGRYGAAVEDVMPDVQRFVGSLVGRG"
     gene            complement(330..1031)
                     /gene="lepB"
                     /locus_tag="VK103_00130"
     CDS             complement(330..1031)
                     /gene="lepB"
                     /locus_tag="VK103_00130"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HLQ81913.1"
                     /translation="MTTTDPAGRADRRPGGRRRRTGWPVWVHLLAALLVMGLLQGFVA
                     RVYRVPSGSMEPTLAVGQRFLVERVSYRFADVQRQDVIVFRIDPQWRGAPDPAVDGPG
                     ELARWTLGLLGVGSGLEDPLVKRVIGLPGDTVACCDEAGRVLVNDVPLAEPYLGPDLA
                     FAAGHHDCRSVQTSPRCFGPIIVPPGRLLVLGDHRASSADSVQHCRGRVGVTECARFV
                     RTDRVVGRAVGLAHD"
     gene            complement(1028..2164)
                     /locus_tag="VK103_00135"
     CDS             complement(1028..2164)
                     /locus_tag="VK103_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81914.1"
                     /translation="MTAPDPSPELLVVEALGVRVAADLSVARVAGEEVAAIRTGWSRA
                     RVNGHRVDATVPPALLASQPSPARLAAALTTAVTLAAIDARAGELVMLHAAGVADPAT
                     GATVALVGPSGRGKTTASRALARHWGYVSDETVGVDPVTLAVVPHPKPLSVKASAQPE
                     ATRGAPKVQVGPDEAGLLPAPDHCRLVRVVLLDRTPDHTGPPTVAPVAVTRAVGELTA
                     ELSHLRHWETPLATVAGLLTATGGALRVRYAEAADLVPLLSELVAHPDRELRWWPVPA
                     GTGNSDGAAGAQRWLRRAADEAIADDDTVAVLLSDEDGSRVVVLDGIGTTLWHHTARP
                     RTTADLVSTVVEHHGTPPAGSPTSLVTQTLASLQARGVLHRCEP"
     gene            complement(2161..>2673)
                     /locus_tag="VK103_00140"
     CDS             complement(2161..>2673)
                     /locus_tag="VK103_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HLQ81915.1"
                     /translation="ICRSPFAQLLLQDRLGADVTVTSGGVRALVGEEMDPRSRAALAE
                     RGVDGPVLNEFRASALDAARVSAADLVLTATTTHRDAVVRMTPRALKRTFTLREFAAL
                     APAVGADSASDLVAAVARARTRMTGRADVTDPVGLDFAGYAVVMDELDAVVQQVTDRL
                     LPLLPGSGGL"
BASE COUNT          384 a         1034 c          926 g          329 t
ORIGIN      
        1 ctcctcgccc caccagcgat ccgacgaacc gctgaacgtc gggcatcacg tcctcgacgg
       61 cggccccgta ccgtccggct gcccgggccg ccaactctgc gacgctcgtc gggccggcag
      121 tcagctgccg ccacatgtac cgggccgacc cctccaacgc gagcggggag ccgtcgtcca
      181 cctgcgcggc ctccagggcg agcacgaaca cctgcgcgcc ggtgtcggtg tgggcgatgt
      241 catcgcacag ctcgacgggg atgtcgatgt cgatgctctg cacccgccca gtgtgctgga
      301 tcggaggcgc cggtgtcgac acggcccggt cagtcgtggg cgagtccgac ggcccggccg
      361 accacccgat cggtgcggac gaaccgggcg cactcagtga ccccgacccg accgcgacag
      421 tgctgcaccg agtcggccga ggaggcgcgg tggtccccca gtaccagcaa ccgtccgggt
      481 ggcacgatga tcggaccgaa gcatcgcggt gacgtctgca ccgatcgaca gtcgtggtgg
      541 ccggccgcga acgccagatc cgggccgagg tacggctccg ccaacggcac gtcattgacc
      601 agcacccgac cggcctcgtc acagcaggcg acggtgtcac ccggcagccc gatcacccgt
      661 ttcaccagcg gatcctccaa cccgctgccg acgccgagca gcccgagtgt ccagcgcgcc
      721 agctccccgg gcccgtcgac cgccggatcc ggcgcacccc gccactgcgg gtcgatccgg
      781 aacacgatca cgtcctgccg ctgcacgtcg gcgaaccggt acgacacccg ctcgaccagg
      841 aaccgttgcc cgaccgcgag cgtcggctcc atcgaccccg acggcacccg gtacacccgg
      901 gccacgaatc cctgcagcag ccccatcacc agcagggcgg ccagcaggtg cacccacacc
      961 ggccatccgg tacggcgtcg ccgcccgccg ggccgccggt cagctcgacc ggccgggtcc
     1021 gtcgtggtca tggctcgcac cggtgcagga ctcctcgtgc ctgcagggat gccagcgtct
     1081 gggtgaccag tgacgtcggc gatccggcgg gcggggtccc gtgatgctcg acgacggtgg
     1141 acaccaggtc ggccgtggtg cggggccgtg ccgtgtggtg ccacagggtc gtcccgatgc
     1201 cgtccagcac caccacccgg ctgccgtcct cgtcggacag cagcacggcg acggtgtcgt
     1261 catcggcgat cgcctcgtcg gcagcgcgcc gcagccaccg ctgcgcaccg gcggccccgt
     1321 cggagttccc ggtcccggcc gggaccggcc accagcggag ctcccggtcg gggtgggcga
     1381 ccagctcgga cagcagcggt accaggtcgg cggcctcggc gtagcgcacc cgcagtgcgc
     1441 caccggtggc ggtgagcagc ccggcgacgg tggccagcgg tgtctcccag tgccgcaggt
     1501 ggctgagctc ggccgtcagc tcccccacag cccgcgtgac ggcgaccggg gccaccgtgg
     1561 gaggaccggt gtggtcgggt gtgcggtcca gcagcaccac ccgcaccaac cggcagtggt
     1621 ccggggcagg caacaggccg gcctcgtcgg gcccgacctg caccttgggt gcgccccggg
     1681 tcgcctccgg ttgggcagat gccttcaccg acagcggttt ggggtgcggt acgaccgcca
     1741 atgtcaccgg atcgaccccg acggtctcgt cgctcacata gccccagtgg cgggccagtg
     1801 cccggctggc cgtcgtcttg ccgcgccccg acggtcccac cagcgcgacg gtcgccccgg
     1861 tcgccgggtc ggccaccccg gcggcatgca acatcaccag ttcgccggcc cgggcatcga
     1921 tcgcggcgag ggtgacggcg gtggtcagtg cggcggccag ccgggccggc gacggttgcg
     1981 acgccagcag tgccggtggc accgtggcgt cgacgcggtg cccgttgacc cgggcccggg
     2041 accagccagt acggatcgcg gccacctcct caccggcgac ccgggccacc gacaggtcgg
     2101 cagcaacccg taccccgagc gcctccacga cgagcagctc gggagacggg tcgggagccg
     2161 tcacaaccca cctgacccgg gcagcagcgg cagcagtcgg tccgtgacct gctgcaccac
     2221 ggcgtccagc tcgtccatca cgacggcata gccggcaaaa tccaacccga ccgggtcggt
     2281 cacgtccgcg cgtccggtca tccgggtgcg ggcgcgggcg accgcggcca ccagatcgct
     2341 cgccgagtcc gcccccaccg cgggtgcgag cgccgcgaac tcgcgcaggg tgaacgtccg
     2401 cttcagcgcc ctgggcgtca tccggaccac cgcgtcccgg tgggtggtgg tagcggtcag
     2461 caccagatcc gccgcggaga cccgtgccgc gtcgagcgcg ctggcgcgga actcgttgag
     2521 caccgggccg tccacgcctc gttcggccag tgcggccctg ctgcgcggat ccatctcctc
     2581 ccccaccagc gcgcggacac cgccactggt caccgtgacg tcggcgccga gccggtcctg
     2641 cagcaacaac tgggcgaacg gggaccggca gat
//
LOCUS       DATGUK010000009         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1289984,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000009 DATGUK010000000
VERSION     DATGUK010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289984"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VK103_00145"
     CDS             complement(<1..380)
                     /locus_tag="VK103_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HLQ81916.1"
                     /translation="MTVSDTSRPDHPSADRPSAGTTPRPQDDLFGHVNGSWLAAATIP
                     DDKPSAGAFDELRDEAEEAVRDIITGLAEDRATLDGEQAKIADLYASFMDLDRVEALG
                     TTPLEGPLAEVDAVRSAGELLALTG"
     gene            complement(438..1733)
                     /locus_tag="VK103_00150"
     CDS             complement(438..1733)
                     /locus_tag="VK103_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81917.1"
                     /translation="MSGSYPPDGPQGPGHGGPGGPQSGGPGGPQYGNQQYGGDQQYGG
                     PQYGGQGGYGPPPGGFDEASGIPSANPAPSGPQSAPQSAGSANFPMYGSPGTQMGSGL
                     TPPPKKSNGLLIGGIIAGAAVVVIAAIVIGVILTRAGGDRNTPTNTVQGYFDALVAGD
                     AEKALSYAKVDPTDKTFLTNDMLTTSNEKAPITNVVVAETSDDAYRVPVSYQIGERRI
                     NAEIRVEEIGNEWKLSAIASEVRVARTGIAPLINGVEAKTDTIYLFPGTYTVSTGNEY
                     VTFPEDESTLTIDEPSQYGGASLSMSPELSQEGLDLMLKEAEKSLQACLAKKELKPEG
                     CPFYANPNLEDGDVKIDMSTFRYTAEGEQFEDVKPRLDYDNPMIAQFNYYPNVKYYAE
                     AERTSNGQKGTFNARVTRSDTNTAQMDFSQETPTFSWVS"
     gene            complement(1922..>2328)
                     /locus_tag="VK103_00155"
     CDS             complement(1922..>2328)
                     /locus_tag="VK103_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HLQ81918.1"
                     /translation="LKDRDTAEDTVIRLRHGEPVVFGRPGEDGVGRYGVRRSPSTGGL
                     EVVEVAEVGVDALVVHDAHAADPSYAFALSRLTTPGALTRSPIGVLRDVDAPAYDDQV
                     RDQLVTAGAPATDPEAQLQDLVRGRDTWTVAG"
BASE COUNT          304 a          803 c          804 g          417 t
ORIGIN      
        1 ccggtcaggg ccaacaactc accggccgag cgtacggcgt caacctcggc cagtggtcct
       61 tcgagcgggg tggtgccgag cgcctcgacg cggtccaggt ccatgaaact ggcgtacagg
      121 tcggcgatct tggcctgctc gccgtcgagg gtggcacggt cctcggccaa cccggtgatg
      181 atgtcgcgga cggcttcctc cgcctcgtcg cgaagctcgt cgaacgcgcc cgcgctgggc
      241 ttgtcgtccg ggatggtggc cgcagcgagc caggagccgt tcacgtggcc gaacagatcg
      301 tcctgcgggc gcggggtggt cccggcgctg gggcggtcgg cggaggggtg gtcggggcgg
      361 ctggtgtcgg aaacggtcac ggccccaacc ttacgggtgg ggccgtgacc gttgcgtcct
      421 cgggtgtggt gctcggctca ggacacccag gagaaggtgg gcgtctcctg ggagaagtcc
      481 atctgggcgg tgttggtgtc cgacctggtc acccgggcgt tgaaggtgcc cttctgcccg
      541 ttcgacgtgc gctccgcctc ggcgtagtac ttgacgttgg ggtagtagtt gaactgcgcg
      601 atcatcgggt tgtcgtagtc cagccgcggc ttgacgtcct cgaactgctc accctccgcg
      661 gtgtaccgga aggtcgacat gtcgatcttg acatcgccat cctccaggtt cgggttggcg
      721 tagaacgggc agccctcggg cttcagctcc ttcttcgcaa ggcaggcctg cagactcttc
      781 tcggcctcct tgagcatcag gtcgaggcct tcctggctga gctccgggct catgctgagg
      841 ctggcgcccc cgtactgcga cggctcgtcg atggtgagcg tcgactcgtc ctccgggaac
      901 gtcacgtact cgttgccggt ggagaccgtg taggtcccgg ggaacaggta gatcgtgtcg
      961 gtcttggcct cgaccccgtt gatcagtggg gcaatgccgg tgcgggccac ccgcacctcg
     1021 ctggcgatcg cggacagctt ccactcgttg ccgatctcct cgacccggat ctcggcattg
     1081 atgcggcgtt caccgatctg gtagctcacc ggcaccctgt acgcatcgtc cgaggtctcg
     1141 gcgaccacga cgttggtgat cggggccttc tcgttgctcg tggtgagcat gtcgttggtg
     1201 aggaacgtct tgtcggtcgg atccaccttg gcgtagctga gcgccttctc cgcatcgcct
     1261 gcgacgagcg cgtcgaagta gccctgcacc gtgttcgtcg gggtgttccg gtcaccaccg
     1321 gcacgggtca ggatgacacc gatcacgatc gccgcgatga ccaccacggc ggcaccggcg
     1381 atgatgccgc cgatcagcag accgttcgac ttcttcggcg gcggtgtgag tccgctgccc
     1441 atctgagtgc cggggctgcc atacatcggg aagttggcgc tcccggccga ctgcggtgcc
     1501 gactgcggtc cactcggtgc cgggttggcg ctcgggatgc cggacgcctc gtcgaagcca
     1561 cccgggggcg gtccgtaacc accttgcccc ccgtactgcg ggcccccgta ctgctggtcg
     1621 cccccgtact gctggttccc gtactgcggg ccgccggggc cgcccgactg cggaccaccg
     1681 ggaccgccgt gccccggccc ctgcgggccg tcaggtgggt agctgccgct cattgtgact
     1741 cccgttcatg taccgctcgt accttgtgat gccgtcgggg gacgtcgccg gcgatccacc
     1801 caccaccgcg tcacggtcgg tcgaccgggg cgtgtgccgg gcgggttcca ccgaccggag
     1861 aatacccgac cgagggtccg ggcgaggact ccggcagcgc gtcgcaccgc ggatctccgc
     1921 ctcagccggc gaccgtccag gtgtcgcgac cgcgcaccag gtcctgcagc tgggcctccg
     1981 gatccgtcgc cggtgcaccg gccgtcacca gttggtcacg gacctggtcg tcgtaggccg
     2041 gggcgtccac gtcacgcagc acaccgatcg gtgaccgcgt caacgcgccc ggcgtggtga
     2101 gccgggacag cgcgaaggcg tacgacgggt cagcggcatg ggcgtcgtgc accaccagcg
     2161 cgtcgacccc gacctcggcg acctccacga cctcgagccc cccggtcgac gggctgcgcc
     2221 gcaccccgta ccggccgacg ccgtcctcgc ccggccgacc gaacacgacc ggctcaccgt
     2281 gccgcaaccg gatcacggtg tcctcggcgg tgtcgcggtc cttcagct
//
LOCUS       DATGUK010000010         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_363470,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000010 DATGUK010000000
VERSION     DATGUK010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363470"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1023)
                     /locus_tag="VK103_00160"
     CDS             complement(169..1023)
                     /locus_tag="VK103_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81919.1"
                     /translation="MSVDDDLLTTRVDALLTDLGSRRGVVVAFSGGVDSALVLAAAVR
                     ALGPARVLAATAVSPALPAAERGPAAAYAAELGVGHEWVETHEIEREGYRANAGSRCY
                     FCKAEMVEVLGPLAADRGWVVATGTNADDALARFRPGIRAADERGAIAPLRDAGLTKE
                     QVRAQSRAWGLSTWDKPAAACLSSRIAYGITITPSVLARVERAEAAVRADWAAADGPV
                     PLTDLRVRDLGEGRARLEVDAAALASLTADPGRQGSALDAVRDSGFDAVELQAFRSGS
                     MNDALTRH"
     gene            1065..1982
                     /locus_tag="VK103_00165"
     CDS             1065..1982
                     /locus_tag="VK103_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81920.1"
                     /translation="MATWEDGPEYAPVERPAEYLEPDAAPLDDAPPRSLRTDDIPAAR
                     PDLQPPDQPLAGLETLAAHDHDEQRDPSEPFQVVQAVMTDSTSAWSAARSTVLTSPVQ
                     QHWAPPSGAPVVAPTGDRASADSANSADPLGSGRRSILTPPPNQPIQLSGQPSPTGAF
                     PTTGSTEWFGPGPRPDEPEPEPITLGGFLAALTPFVLISLLIGGLIPPISPLVFVVAF
                     ALSSRITVAGEGVRRAFAGGALVVSLVAVVTIFTGPGIGEWWSTVGLTSLVVCWAVLL
                     ISAVLVLRALQSGVPVPGGREQARRNTWA"
     gene            1994..>2791
                     /locus_tag="VK103_00170"
     CDS             1994..>2791
                     /locus_tag="VK103_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81921.1"
                     /translation="MTVRDRTYADWLRRRPDAELAATLHRRPDLLHPPPEDLADLAAR
                     ATTASSVARVLRHLDAWQATVVEAVAALGGETTRAEVATILGMPGDRDAVDGAVDELR
                     RWALVWGSQESVRLVREARIASGPYPGGLAPVSARPLGSDEVERALAGIGPAERSLLD
                     RLVWGPPTGRVRDATHQPDDSPVGRLLRLGLLRLHDADTVMLPREVAWRLRSPARLLA
                     DQPSPAPPDLVAGEVEAATGASSGGLRARLAAQGAVATAAELVHDVEA"
BASE COUNT          356 a         1054 c         1019 g          362 t
ORIGIN      
        1 tggccggacg tcggctgccg atggtcacca cggcgaccct gacccggctg ctgccggtcg
       61 gcatcgtgca gctgtcgcga caaccgcaac cggcccaggt ccgctgaacc gccccgccgt
      121 ccgcggcgcg tcagtaccgg gtcagggcgt cagtgccggg tcagggcgtc agtgccgggt
      181 cagggcgtcg ttcatcgacc cggagcggaa cgcctgcagc tcgaccgcgt cgaaaccact
      241 gtcccgtacg gcgtcgagtg cactgccctg ccgccccggg tcggcggtga gactggccag
      301 ggccgccgcg tccacctcga gccgggcccg gccctcaccg aggtcacgga cccgcaggtc
      361 ggtgagcggg acggggccgt cggcggccgc ccagtcggcg cgcaccgccg cctcggcacg
      421 ctccacccgc gccagcaccg acggggtgat ggtgatcccg tacgcaatcc gcgagctcag
      481 gcaggccgcg gccggcttgt cccaggtcga cagcccccag gcacgcgact gggcccggac
      541 ctgctccttg gtcaggccgg cgtcacgcag cggggcgatc gcaccacgtt cgtcggcggc
      601 ccggatgccg gggcggaacc gggccagcgc atcatcggcg ttcgtcccgg tcgccaccac
      661 ccagccgcga tcggcggcca acgggccgag cacctcgacc atctcggcct tgcagaagta
      721 gcagcggctg ccggcgttgg cgcggtagcc ctcccgttcg atctcgtggg tctcgaccca
      781 ctcgtggccc accccgagct ccgcggcgta ggcggcggcc gggccacgtt cggcggcggg
      841 cagggcgggc gacactgccg tcgcggccag cacccgggcg ggtcccaggg cccggaccgc
      901 cgccgccagc accagcgcgg agtcgacgcc accggagaac gcgaccacca cgccacgccg
      961 cgagccgagg tcggtcagca gcgcgtcgac acgggtcgtg agcaggtcgt catccacgct
     1021 caccagcgta ggccgtgcgg cagcacggca gtacgatggt cggcatggcc acgtgggagg
     1081 acggaccgga gtatgcgccg gtcgagcgac cggcggagta cctggagccg gacgccgcac
     1141 cgctggacga tgccccgccc cgcagcctgc gcaccgacga catccccgcg gcacgaccgg
     1201 acctgcagcc gcccgaccag ccgttggccg gcctcgagac gttggccgcc cacgaccacg
     1261 acgaacagcg cgacccgtcc gaaccgttcc aggtggtgca ggcggtgatg accgactcca
     1321 cctcggcctg gtcggccgcc cgttccaccg tcctcacctc accggtgcag cagcactggg
     1381 cgccaccgtc gggcgcgccc gtggtcgccc cgaccggcga ccgggcctcc gctgacagcg
     1441 ccaacagcgc cgacccgctc ggttccggac gccgcagcat cctcacccca ccgccgaacc
     1501 agcccatcca gctgagcggg cagccgtcac cgaccggggc gttcccgacc accggcagca
     1561 ctgagtggtt cggtccggga ccacgacccg acgagccgga gccggagccg atcacgctcg
     1621 gcgggttcct ggccgcactc accccgttcg tgctgatcag cctgctcatc ggcggcctga
     1681 tcccaccgat ctcaccgctg gtgttcgtcg tcgccttcgc actgtcgtcc cggatcacgg
     1741 tcgccgggga aggggtccgc cgcgcgttcg caggcggggc gctcgtggtc agcctggtcg
     1801 cggtggtgac gattttcacc gggccgggga tcggtgagtg gtggtcgacc gtcgggttga
     1861 cgagtctggt ggtgtgttgg gcggtgctgc tgatctcggc cgtgctcgtg ctgcgtgccc
     1921 tgcagtcggg tgtcccggtc ccgggcggtc gcgaacaggc ccgccgcaac acgtgggcct
     1981 gagcgggacg gccgtgacgg tacgggaccg cacctacgcc gactggctgc gtcgacgccc
     2041 cgacgctgag ctcgccgcca ccctgcatcg ccgacccgac ctgctgcatc cgcccccgga
     2101 ggacctggcc gacctggcgg cccgtgccac cacggcgagc tcggtggcac gggtgctgcg
     2161 ccacctcgat gcctggcagg cgaccgtcgt ggaggctgtc gccgcactcg gtggcgagac
     2221 cacccgcgcc gaggtcgcca cgatcctggg gatgccggga gaccgggatg cggtggacgg
     2281 cgccgtcgat gaactgcggc gctgggccct ggtgtggggc tcacaggaat cggtacggct
     2341 ggtccgggag gcccgcattg cgtccgggcc gtacccgggt gggttggcgc cggtgtcggc
     2401 ccgcccgctg gggtcggacg aggtggagcg ggcgctggcc gggatcggtc cggcggaacg
     2461 gtcactgctg gaccggttgg tctggggacc gccgaccggc cgggtgcgtg acgcgacgca
     2521 ccaaccggac gactccccgg tgggccggtt gctccggctg ggcctgctgc gactgcacga
     2581 cgccgacacc gtgatgctgc cccgcgaggt cgcttggcgg ctccggtcgc cggcgcgcct
     2641 gctcgccgac caaccctccc ctgccccacc cgacctggtc gccggtgagg tcgaggccgc
     2701 cactggcgca tcgagcggtg ggctgcgggc ccgactggcc gcccaggggg cggtcgcgac
     2761 cgcggccgag ctggtgcacg acgtggaggc c
//
LOCUS       DATGUK010000011         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_480386,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000011 DATGUK010000000
VERSION     DATGUK010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480386"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..2491)
                     /locus_tag="VK103_00175"
     CDS             complement(110..2491)
                     /locus_tag="VK103_00175"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HLQ81922.1"
                     /translation="MSSLLATARRSLSTRLREHSVLGRTFGTSRIRGPATIHDLPVAD
                     RWRWIAVDAVALLVLLALVCVAFWPLYGVGWLWMAGMGGAVLGVVIGIAGAWQRWPAW
                     IVAVVAACAHLLFGSMLAMPQLAGASVLPTARTLSGLVFGVVQTWKRVLTMDAPIGTT
                     DNLLVLPLLTLLVAGILGVTLATRSGRPGLAWLPPALALVVAIAFGLQTGYRPVAVGI
                     AFFVVALLWTAYRRDYQRQTLLTRRRRYSLRTVLSAVGMLLVAGLVAGLVSPLVTPDT
                     RHVLRDTVEPPLDVRQWPSPLQSFRSNVKDHRHDVLFTVDGLPTGAGIRLATLDAYDG
                     ITVNASSSVSGAPGGGTYKRIGSRVPDDTPGTSISYTVTIGDYRGRWVPTVGKTLALE
                     FTGDRDDELSGQFFYNRTTGSGVSTLPLQAGDRYRVDAVVPVQPNRSEVRTASAGDVD
                     LPSASPIAEELAARAREWTAGVTTDGEAAIRLEEKLRQAYYSHGLANDVPSLPGHSSY
                     RMMTLMTDHEGRPVGDEEQYAVAMALMAREVGLPSRVVYGYRPTSNGTVEVVGDNVSA
                     WVEINFDRLGWVEFFPTPDKSRALPQDTTHAESKPRPQVENPPPPPERPEEPPPDNTP
                     PLPGEEDQEDPAAIDWAAVLRVVLAVLVPLLLIAGPLLAVMAIKARRRRLRRGADSPF
                     DRVAGGWAELLDRACDLGAAPSRAATRSENAAAVAARFPTALSVTPPSVLAHRADWAS
                     FSGHPPTAAMVDEYWSDVTATGQAMARTVSPWRRFVAAISLRSLLGRDKAARQ"
     gene            complement(2488..>3001)
                     /locus_tag="VK103_00180"
     CDS             complement(2488..>3001)
                     /locus_tag="VK103_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81923.1"
                     /translation="LTTGNRLRAVNARRTLDELCGVEAVSTAPRPRTARSDEVDAPGP
                     AAALRTLAARREARRQREAGDPVLRLSREVQHAEPNASLVSLVTGSRPTVPQLHQAGN
                     TFDPDVRTLLVRVELGAELSVQTIGAVSVVTLGDLDDLPRGVRRATLLAPSAGGAGGA
                     GAAGSVGASA"
BASE COUNT          442 a         1130 c         1047 g          382 t
ORIGIN      
        1 gtccccggcc gtgatacctg gcgggaaccc cgcatcctgg ctgctcatcc cactccctcc
       61 ggccacaggt ggccaccgtg cacgatgcca cacctgtggc gaccaaatcc tactggcgcg
      121 ccgccttgtc ccggccgagc agggaccgca acgagatcgc ggcgacgaac cggcgccacg
      181 gcgacacggt gcgggccatc gcctgtccgg tcgcggtgac gtcgctccag tactcgtcga
      241 ccatcgcggc cgtcggcggg tgcccactga acgacgccca gtccgcccgg tgcgcgagca
      301 cgctcggcgg cgtcaccgac aaggcggtgg ggaaccgggc ggccacggcg gcagcgttct
      361 cgctccgggt cgccgcccgc gacggtgccg ccccgaggtc gcaggcccgg tccaacagct
      421 cggcccaccc accagccacc cggtcgaacg ggctgtcggc cccacggcgc aaccgtcggc
      481 gtcgtgcctt gatcgccatc accgccagca gcggtccagc gatcagcagc aacggaacga
      541 gcaccgccag caccacccgc agcaccgcgg cccagtcgat cgcggccgga tcctcctggt
      601 cctcctcgcc cggcagtggc ggggtgttgt cgggcggcgg ctcctccggc cgttccggcg
      661 gcgggggcgg gttctcgacc tgtggccgcg gcttcgactc ggcgtgcgtg gtgtcctgcg
      721 gcagcgcccg cgacttgtcc ggggtcggga agaactccac ccagccgagc cggtcgaagt
      781 tgatctccac ccaggcgctg acgttgtcgc cgacgacctc cacggtcccg ttcgaggtgg
      841 gtcggtagcc gtacaccacc cggctcggca gcccgacctc acgggccatc agtgccatcg
      901 cgacggcgta ctgctcctcg tcgccgaccg gccgaccctc gtggtcggtc atcaacgtca
      961 tcatccggta gctgctgtgg cccggcaggg acgggacgtc gttggccagc ccgtggctgt
     1021 agtaggcctg tcggagcttc tcctccaacc tgatcgccgc ctccccgtcg gtggtgacac
     1081 cagcggtcca ctcccgcgcc cgggcggcca gttcctccgc gatcgggctg gccgagggca
     1141 ggtccacgtc accggccgag gcggtacgga cctcgctgcg gttgggctgc acgggcacca
     1201 ctgcgtcgac ccggtagcgg tcgccggcct gcagcgggag cgtgctcacc ccggagccgg
     1261 tggtgcggtt gtagaagaac tgcccggaca actcgtcgtc gcggtccccg gtgaactcca
     1321 gggccagtgt cttgccgacg gtgggcaccc agcgcccgcg gtagtcaccg atcgtgacgg
     1381 tgtagctgat cgaggtgccg ggggtgtcgt cggggacccg gctgccgatc cgcttgtacg
     1441 tcccaccacc gggggcaccg gagaccgagc tggaggcgtt gacggtgatg ccgtcatagg
     1501 cgtccagggt ggccagccgg atcccggcgc cggtgggcag gccgtccacg gtgaacagca
     1561 cgtcgtggcg gtggtccttg acgttgctgc ggaaggactg cagcgggctg ggccactgcc
     1621 gcacgtccag cggcggctcg accgtgtcgc ggagcacgtg gcgggtgtcc ggggtcacca
     1681 acggggacac cagtccggcc accagcccgg cgacgagcag catgccgacg gcgctcagca
     1741 ccgtccgcag gctgtagcgg cgtcgccggg tgagcagcgt ctgacgctgg tagtcacgcc
     1801 ggtaggccgt ccacagcagc gccaccacga agaacgcgat cccgaccgcg acgggtcggt
     1861 acccggtctg cagcccgaac gcgatcgcga ccaccagcgc cagcgcgggc ggcagccacg
     1921 ccagcccggg tcggcccgac cgggtggcca gcgtgacacc gaggatgccg gccaccagca
     1981 gggtgagcaa cggcagcacc agcaggttgt cggtggtgcc gatcggggcg tccatggtga
     2041 gcacccgctt ccaggtctgc accacgccga acaccagacc ggagagggta cgggcagtgg
     2101 gcagcaccga tgccccggcc agttgcggca tcgccagcat cgacccgaac agcaggtggg
     2161 cacacgcagc gaccactgcc acgatccagg ccggccagcg ttgccaggct cccgcgatgc
     2221 cgatcaccac gccgagcacc gcaccaccca tcccggccat ccacagccag ccgacgccgt
     2281 acagcggcca gaacgccacg cacaccagcg cgagcagcac cagcagcgcc accgcgtcca
     2341 ccgcgatcca gcgccaccgg tcggcgaccg gcaggtcgtg gatcgtcgcc gggccccgga
     2401 tccggctggt gccgaaggtg cgcccgagca cgctgtgctc ccgcaacctg gtggacaggc
     2461 tgcgacgggc ggtcgccagc agtgaactca tgcgctggcc cccaccgacc cggccgcccc
     2521 ggcaccaccg gcaccaccgg ccgacggagc gagcagggtg gctcggcgga caccacgggg
     2581 caggtcgtcg aggtcaccga gggtgaccac gctgaccgca ccgatcgtct gcaccgacag
     2641 ctcggcgccc agctcgaccc gaaccaacag ggtgcgcacg tccgggtcga aggtgttgcc
     2701 ggcctggtgc agctgcggca cggtcggacg cgacccggtc accaacgaca ccagcgaggc
     2761 gttcggttcg gcatgctgca cctcgcggga cagccgcagc accgggtcac cggcctcccg
     2821 ctgtcgtcgc gcctcccgcc gtgcggccag ggtccgcaac gccgcggccg ggccgggggc
     2881 gtccacctcg tcggagcggg cagtgcgcgg tcgcggtgcc gtgctgaccg cctccacccc
     2941 gcacaactcg tccagggtgc ggcgggcatt caccgcccgc agccggttgc cggtggtgag
     3001 c
//
LOCUS       DATGUK010000012         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_364343,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000012 DATGUK010000000
VERSION     DATGUK010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364343"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VK103_00185"
     CDS             complement(<1..249)
                     /locus_tag="VK103_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81924.1"
                     /translation="MIVSFGRRIVAVPVAVFALLVGACSGGQPLSTEPAPADAVSAAS
                     VEVPTVEPGTPELPVTFRSDDSVEVEVTSLDRVLVLDDA"
     gene            complement(480..2426)
                     /locus_tag="VK103_00190"
     CDS             complement(480..2426)
                     /locus_tag="VK103_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HLQ81925.1"
                     /translation="MTPDTRPNIIVVLCDDLGFGDVGVLFQNQLRRTDPTAPSLRTPR
                     IDQMAEEGLTLTGMYCSAPVCAPSRASLLTGTTQGHADLRDNAFDRALEDSENMATVL
                     RSRGYATAAIGKWGLQGTADPGGELPGAWPAYPTRRGFDEFYGYVRHADGHEHYPKEG
                     LHRGRKEVWHNDTEVSDGLDGCYTTDLFTARAKQWITEQCVDTGERQPFFCYLAYDTP
                     HAVTELPPCAYPDGAGANGGVQWIGDAGNMINTAGGEPDSYRHPDSVAPPGCEPWPDV
                     YQRYATVVRRIDDCVGDLLTLLGDLGIDEETLVIFTSDNGPSIESYLTEPYRADFFSS
                     YGPFDGIKRDCWEGGVRVGALVRAPGLVDAGRSSAAPVQFHDLLPTFAELAGATPPAR
                     TDGVSLVPLLTGRGEQRPSTVYTEYAVKGATPDYEAFHPSRRGRLRGQMQLIRLGDHV
                     GVRYDVRSADDPFEIYDVLADPKQEHDLRDRMPELHHQMLSAVPRMRMPHADAPRPYD
                     DELATPVGGATSGAPGPVERRTYTDSHPWVPLLDTVEPTTSTTVDWPVDDQPVPPGTT
                     AMFYTGELFVEADGAHRFEVTGPGAVVLRIHDALVAEGPSPAAPSAATVPVTGTMRLA
                     RGVHPFRLYVTGAEPPRVSWARVE"
BASE COUNT          371 a          895 c          878 g          391 t
ORIGIN      
        1 ggcgtcgtcg agcaccagca cccggtccag actggtcacc tcgacctcga cgctgtcatc
       61 gctgcggaag gtcaccggca gctcgggcgt cccgggttcg acggtgggga cctccaccga
      121 cgccgccgac accgcatccg cgggcgccgg ttcggtcgac aggggttggc cgccgctgca
      181 ggcaccgacg agaagtgcga acacggcgac ggggacggcc acgatcctac gtccgaacga
      241 cacgatcatc cttccacccg agtattacct cggtctccga gttattaggc cagcctaagc
      301 taacatcccg gagggagtcg tgcacatcct gagatctcct gcctggtcaa ggcccatccc
      361 gatccaggag aacgtcaacc aggtgcggtc gggtctgaac cacgatggcc gcctgggcgc
      421 cacggcgagt ctgacggacc cgggcccgtc cgcaggtcga gcgaagtcga gcccgacgac
      481 tactccaccc gagcccagct cacccgtggc ggctcggcac cggtgacata gagccggaac
      541 gggtgcaccc cgcgcgccaa ccgcatcgtc ccggtgaccg gaactgtcgc cgccgacggt
      601 gcggccgggc tcggcccctc cgccaccagg gcgtcatgga tgcgcagcac gaccgccccg
      661 ggcccggtca cctcgaaccg gtgggcgccg tcggcctcca cgaacagttc accggtgtag
      721 aacatcgcgg tggtgcccgg cggcaccggc tggtcgtcga ccggccagtc gaccgtggtc
      781 gaggtcgtcg gctcgaccgt gtcgagcagc ggcacccacg ggtggctgtc ggtgtaggtc
      841 cggcgctcca ccgggccggg ggcgccactg gtcgcgccgc ccacgggcgt ggcgagctcg
      901 tcgtcgtacg gccggggtgc gtcggcgtgt ggcatccgca tgcgtgggac cgcggacagc
      961 atctggtggt gcagctcggg catccggtcc cgcagatcgt gctcctgctt gggatcggcg
     1021 agcacgtcgt agatctcgaa cgggtcgtcg gcgctgcgca cgtcgtagcg cacgccgaca
     1081 tggtcgccca acctgatcag ctgcatctgc ccccggagcc gcccacgccg ggacgggtgg
     1141 aacgcctcgt agtccggggt ggcccccttg acggcgtact cggtgtacac cgtcgacggt
     1201 cgctgctcgc cccgaccggt gagcagcggc accaacgaca ccccgtcggt acgggccggc
     1261 ggggtcgcgc cggcgagctc ggcgaaggtc ggcagcaggt cgtggaactg caccggtgcc
     1321 gcgctgctgc gacccgcgtc gaccagcccg ggggcacgca ccagcgcgcc gacccggaca
     1381 ccaccctccc agcagtcgcg cttgatgccg tcgaagggcc cgtacgagga gaagaagtcc
     1441 gcccggtacg gctccgtcag gtagctctcg atgctgggcc cgttgtcgga ggtgaagatg
     1501 accagggtct cctcgtcgat gccgaggtca cccagcaggg tgagcaggtc gccgacgcag
     1561 tcgtcgatcc ggcgcaccac ggtggcgtag cgctggtaga cgtcgggcca cggctcgcac
     1621 cccgggggtg caacgctgtc cgggtgccgg tagctgtccg gctcgccacc ggcggtgttg
     1681 atcatgttcc cggcgtcacc gatccactgc acgccaccgt tcgcgccggc gccgtcgggg
     1741 tacgcgcacg gcggcagctc ggtgaccgcg tgcggggtgt cgtaggccag gtagcagaag
     1801 aatggttgcc gctcccccgt gtccacgcac tgctcggtga tccattgctt ggcccgggcg
     1861 gtgaacaggt cggtggtgta gcaaccgtcc aggccgtcgg aaacctcggt gtcgttgtgc
     1921 cacacctcct tgcgcccacg gtgcagtccc tccttcggat agtgctcgtg gccgtcggca
     1981 tgccggacgt acccgtagaa ctcgtcgaac ccacgccggg tggggtaggc cggccaggca
     2041 cccggcagct cgccaccggg atccgcggtc ccctgcaggc cccacttccc gatcgcggcg
     2101 gtggcgtacc cgcgggaccg cagcacggtg gccatgttct ccgagtcctc cagagcccga
     2161 tcgaaggcgt tgtcccgcag gtcggcgtga ccctgggtcg tgccggtcag cagggaggcc
     2221 cgggacggcg cgcacaccgg cgccgagcag tacatcccgg tgagggtcag gccctcctcg
     2281 gccatttggt cgatccgtgg cgtgcgcagt gacggcgccg tcggatcggt ccgccgcagc
     2341 tggttctgga acagcacgcc cacgtcgccg aatccgaggt cgtcgcacag caccacgatg
     2401 atgttcggcc tggtgtcggg ggtcacgtcc gttgtcgtcc ttccatgcca tcggtcattg
     2461 tcgtcgatgg ggtcgtggtc atcccttgac cgctcctgcg atgctctcca cgaagtagcg
     2521 ctgcaacacc aggaa
//
LOCUS       DATGUK010000013         4161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_131494,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000013 DATGUK010000000
VERSION     DATGUK010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131494"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VK103_00195"
     CDS             <1..886
                     /locus_tag="VK103_00195"
                     /inference="COORDINATES: protein motif:HMM:NF018829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HLQ81926.1"
                     /translation="LGASAGVAEREGIVDANLWTGFVWADEPRCTAAVLVTATDESAG
                     RAAVEELAELYWDHRARFGIASPRHGSLDQALDHVLAGAARPCFISDSGDNVTAGASG
                     DVTYALRSVLDRDDLATAGRTVLVAGLFDPAAVARAVAAGVGAVLTEPIGAWLDDRFA
                     PPVAGPWTVEGLVRGPLDDEVTAAVLRRGTVSVSVQTRRVLFGTLQDSSAWPFDLRGL
                     SPVDTADVDAVIVKNGYLLPSQEARAGSSFLAITPGATDVDTGRLGHVRVQRPLHPLD
                     DDFQPVLSAEVVPAPAGS"
     gene            complement(899..1711)
                     /locus_tag="VK103_00200"
     CDS             complement(899..1711)
                     /locus_tag="VK103_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81927.1"
                     /translation="MSRLSVEVIGNHATERTSVLDAVASLPRSFRLGSPGDVAAICAG
                     PDWLPATHGAITDGARAVVVMEPTPVPVEAIAPVVAAADSAGTRVVLARGWASNPAVA
                     VLRDRHLDAGPTTLVDSHAEVGPDTSADHALHAQLDLVGCLFGAEIELTDLVRTTSGH
                     LASGRLRRTDRAPVQLFGSRSVAGDGSIRVRELLARGRRALTLPDGRTARPGQAWLSD
                     AAGHQLLPTIHESSTRRVFGLLRAAIDAGVTGSEDLSAFAEVQRTVAGSRAH"
     gene            complement(1708..2781)
                     /locus_tag="VK103_00205"
     CDS             complement(1708..2781)
                     /locus_tag="VK103_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLQ81928.1"
                     /translation="MTVGFIGSGSVVRSIHLPTLARLPDLFRVGHICDVDLAAARAVA
                     GTVGARWSSSVTELLQDSAVDVVAVCSPHQLHTDHVVAACEAGVRAILCEKPLATSVA
                     DAARIAATARRTGTSVILGSMHLFDPAWAFLRQHFGPLRGDGGGGVVGDGGGRGGATT
                     VRSNIMLPFNDRFERWATEPDGTGPLPEVDHSTAAGRAASITIQLLGLAIHDLPLVRA
                     VAPQWREVEVISADVMEPLGYRVQLRAGPTLIELSGGFRDHWQTEWEFAASDEHSIAR
                     VEFTPSFVQAGSGTARITDADTTWDFPGSDSNGYEGEWRSLAQTTMSDANGSARLEGY
                     VADLEFALVVADAVATHIEEEQQ"
     gene            complement(2903..3751)
                     /locus_tag="VK103_00210"
     CDS             complement(2903..3751)
                     /locus_tag="VK103_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLQ81929.1"
                     /translation="MYTVLSPQRLSLDLDQDSMVRAARAGGYDGVEPLLDHFVASSDE
                     ADRVTELVREAGLRWGPAALPATIGPETSESGFGAIVDALRQAAPRLARAEVTTLSTW
                     IAPAGDRLAYSDALRFHRERLTVLLPILRDHGLRLSMEYVGPPTWRVPHRYDFVHSYQ
                     GMRELLDGLSDPDAFGVTLDSFHWYAGGEAAQDIAVAHDILVVDLNDAIPGVPAEEQL
                     DLQRAQPGATGVIDLDEFLAAVRATGFSGPVHSEVMSDSLDHQPPEQRIAEASRALRR
                     TLAQQA"
BASE COUNT          621 a         1379 c         1525 g          636 t
ORIGIN      
        1 gctcggcgcg agtgccgggg tcgcggagcg ggagggcatc gtcgacgcca acctgtggac
       61 agggttcgtg tgggccgatg agccgcggtg cacggcggcg gtcctggtga cggccaccga
      121 cgagtcagca gggcgcgccg cggtcgagga gctggccgag ttgtactggg accaccgcgc
      181 gaggttcggg atcgccagcc ctcgtcacgg cagcctggac caggcgttgg atcatgtgct
      241 cgccggggcc gcgcggccat gcttcatcag cgattccggc gacaacgtca ccgccggtgc
      301 cagcggggac gtcacctacg cgttgcggtc cgtgctggac cgggacgatc ttgccaccgc
      361 cggcaggacg gtgctggtcg cggggctgtt cgatccggcc gcagtggccc gagcggttgc
      421 ggccggcgtc ggtgcggtgc tcaccgagcc gatcggtgcg tggctcgacg accgtttcgc
      481 accgccggtt gccggcccgt ggacggtgga gggactggta cggggaccgc tggacgacga
      541 ggtcaccgcc gctgtgctgc gccgaggaac ggtgtcggtc agcgtgcaga cccggcgcgt
      601 cctgttcggc accctgcagg acagctcggc ctggccgttc gacctccgcg ggctgtcacc
      661 ggtggacacc gccgacgtcg acgccgtcat tgtgaagaac ggatacctgt tgccgagcca
      721 ggaggctcgg gccggcagtt ccttcctcgc gatcactccg ggtgccaccg acgtcgacac
      781 cggccgactg ggccacgtgc gggtgcagcg gccgctccat ccgttggacg atgacttcca
      841 gccggtactg tcggcggagg tcgtcccggc tcccgcgggc tcgtgagccg ggccggtctc
      901 agtgggctcg ggagccggcc acggtgcgct ggacctccgc gaacgcagac aggtcctcgc
      961 tgccggtgac gccggcatcg atcgccgcgc gcagcaaccc gaagacgcgt cgggtgctgc
     1021 tctcatggat ggtcggcagc agctggtggc cggcggcatc gctgagccac gcctgtcccg
     1081 gccgggcggt tcggccgtcg ggcagcgtga gcgcccgacg gccccgggcc agcagctcac
     1141 ggacccggat cgaaccgtcc ccggcgaccg aacgtgagcc gaacagctgg accggggcac
     1201 ggtcggtacg tcgcaggcgc ccgctggcca gatgaccgga cgtcgtccgc accagatcgg
     1261 tgagctcgat ctcggccccg aacaggcatc cgaccaggtc gagttgtgcg tgcagggcgt
     1321 ggtccgcaga ggtgtccgga ccgacctcgg cgtggctgtc cacgagtgtc gtcggaccgg
     1381 catcgaggtg gcggtcccgc agcacggcga ccgccgggtt gctggcccac ccgcgggcca
     1441 gcaccacgcg ggtcccggcc gagtcggcag cggccacgac cggagcgatg gcctcgacag
     1501 ggaccggggt gggctccatc accacgaccg cacgggcgcc atcggtgatg gcgccgtgcg
     1561 tggcagggag ccagtccggt ccggcacaga tggcagcgac gtcaccgggg ctccccaggc
     1621 ggaacgaccg tggcaggctg gcgacggcgt ccagcaccga tgtgcgctcg gtggcgtggt
     1681 tgccgatgac ctcgaccgac agacggctca ctgctgctcc tcctcgatgt gcgtggcgac
     1741 cgcgtcggcg accaccaggg cgaactcaag gtcggcgacg tagccctcga gccgtgccga
     1801 accgttcgcg tcggacatcg tggtctgtgc gagggaacgc cactcgcctt cgtatccgtt
     1861 gctgtccgag ccggggaagt cccaggtggt gtctgcatcg gtgatccgcg cggtgcccga
     1921 ccctgcctgg acgaacgatg gggtgaactc gactcgtgcg atcgagtgct cgtccgaggc
     1981 ggcgaactcc cactcggtct gccagtggtc gcggaacccg ccggagagct cgatcagggt
     2041 ggggcccgcc cgcagctgga cccgatagcc gagcggctcc atcacgtcgg ccgagatgac
     2101 ctcgacctcg cgccactgcg gtgccacggc gcggaccagg ggcagatcgt ggatggccag
     2161 ccccagcagc tggatggtga tcgatgcggc ccgtccggct gcggtcgagt gatcgacctc
     2221 ggggaggggg ccggtgccgt cgggctcggt ggcccagcgt tcgaaacggt cattgaacgg
     2281 gagcatgatg ttcgaccgga cggtggtcgc tccacctctt cctcctccat caccaacaac
     2341 accaccaccg ccgtcgccgc gcagcggacc gaagtgttgc cggaggaatg cccaggccgg
     2401 gtcgaacagg tgcatcgacc cgaggatcac cgacgtcccc gtccggcgag ccgtggcggc
     2461 gatccgtgct gcatcggcga cgctggtcgc cagcggcttc tcgcagagga tggctcgcac
     2521 ccctgcctca cacgcagcga cgacatggtc ggtgtgcagc tggtgtgggc tgcacaccgc
     2581 caccacgtcg accgcggagt cctgcagcag ttcggtgacc gacgacgacc accgggcgcc
     2641 gacggttccg gcgaccgctc tcgcggcagc aaggtcgaca tcacagatgt ggccgacccg
     2701 gaacaggtcg ggaaggcgcg cgagggtcgg cagatggatg cttcggacca ctgatccgga
     2761 gccgatgaac ccgacggtca acggggctgc cgggccaggg gtgttcccct gtcctgccgg
     2821 gacgtctgac gatgctggct gcacgggcgg cgtgatggtg atggttcctc ctcggacgga
     2881 cggtgcgcgg gaacggccgg ggtcatgcct gctgtgccag ggtgcgccgc agagctcggc
     2941 tggcctccgc gatgcgctgc tccggcggct ggtggtccaa cgagtcgctc atcacctcgc
     3001 tgtggacggg accgctgaat ccggtggcgc ggaccgccgc gaggaactcg tcgagatcga
     3061 tcaccccggt cgcgccgggt tgcgcacgct ggaggtcgag ttgctcctcc gcaggcacgc
     3121 cggggattgc atcgttcagg tcgacgacca ggatgtcgtg ggcgaccgcg atgtcctgtg
     3181 cggcctcgcc accggcgtac cagtggaagc tgtcgagcgt cacgccgaag gcgtccggat
     3241 cgctgaggcc gtcgagcagt tcgcgcattc cctgatagct gtggacgaag tcgtaccgat
     3301 ggggcacccg ccaggtcggc gggcccacgt actccatcga gagccgcagg ccatggtcac
     3361 gaaggatcgg gagcaggacg gtgagccgct cccgatggaa ccgcagggcg tcggagtagg
     3421 cgagacgatc acctgccggc gcgatccagg tcgacagggt cgtcacctcg gcccgggcaa
     3481 gtcgtggggc agcctggcgc agtgcgtcga cgatggcgcc gaatccggac tccgacgtct
     3541 cgggcccgat cgtcgcaggc agggcggcag ggccccagcg caggcccgcc tcccggacca
     3601 gctcggtcac ccggtccgcc tcgtccgagc tggcgacgaa gtggtccagc agtggttcga
     3661 cgccgtcgta tccgcctgct cgggcggccc tgaccatcga gtcctgatcc aggtcgaggg
     3721 acaggcgttg tgggctgagg acggtgtaca cgatgggtca cctcaccttt ggaacgtgtg
     3781 cccgcgacgt ggccggggcc cggatggaac atcgatcgga accgctgtgt ggcgacccta
     3841 gcccgttgcg taacagtgcg caatcatttc ccgagcagat cccagtgagg gtttcacctc
     3901 ggggtgggcc gatttcactc gtcctgacac ggcgtcttcg tgtcaccagg gtccgtactg
     3961 gcatgtggtc gatcgcacgt gttgcgcgac ggaatgccaa aaatgcgcaa acgtaggtac
     4021 agtgtgcgca acatctgggc aggcgtcgtg ggatgccggt ccgcccggtg gatctggtga
     4081 acgtcgaaag ggttgtcatg gccaggattg agaccgttcc gaagccgagc atcgcgatgc
     4141 cggaccgtcg accggaggtc c
//
LOCUS       DATGUK010000014         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1524346,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000014 DATGUK010000000
VERSION     DATGUK010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524346"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..2569
                     /locus_tag="VK103_00215"
     CDS             167..2569
                     /locus_tag="VK103_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81930.1"
                     /translation="MNRPADVESALQRLKGWRDRAVAGRQPGAETIRDIHLSMVARSG
                     RRIPSAIAEVLPQSLKGFAGQMARAMVDRPGPGAQPGPAGPPVQGGPPPTQGGRPGAP
                     PHHHPQQGAPGHVSNVGGARDVPLGGVRLGGDPAEPTRSGPAEPTRSAPPVSAASGPR
                     PPGSDGWGGYPPASGSSASVPPDSAASASSASVPPSAMSPDAEAAVRPGGPPARPGTS
                     GSGTSGSGTSGSAPAAPPPKETDEERQQRLAAEWEKFAPMDFSELGDEPGRIQAAAAP
                     GGGVRLRWQPVDDDPGHTVYRVVSGDDYAPYDPQNADFVEITTDTACVDDREFRAAIR
                     HLQVWRNSGATEAEAYANQPVMHARVPLVAPVTGVDLREDEGRVIGQWSVFPGVKRVQ
                     VYRIPIERAQSGFGDPAFRILADDANLGGFVDHGADRGKRYVYQFFCEAEHDGLVRLS
                     APTTRQLLVSAVLEPVTDLEIELDNSATDAQFDLTWTNPPAGRVVVYRTEHPPRAGAD
                     AEARAESALPQMGLPDDARLVHPVVAEGDRSAMRTVPWPRGWVRTYFTPVTVLDGRVQ
                     VGNHIPAVRTMPVTDPVVVERTHTQVLKFGWPRGASYVSVFLGARDQPAEELLETAQR
                     MDISADEYTRLGGMHFSRTLPSSGCSVHMVGVSYSNGERIESAPVSVDYPGLLRLEYT
                     VEHRRGRLNRGPMTLQLRIRSELKLTHAPPFLVVFNPDRFPLESGDGDPVDVVLDDGR
                     ANMPVRRFVPDGLDPEWSQQLWQAMPPGPGFVRLFLDLPPERLQTVALLDPDINQLIV
                     RG"
BASE COUNT          367 a          912 c          987 g          343 t
ORIGIN      
        1 cccgactacg agctggccgt gctcgaccag agcaccgcgc cgacggaggc gccgcaggcc
       61 aagctcggca gtgaggacgc cgcgacgtgg accttctgac agcggcgccg gcggtgccgc
      121 accgggcacc ggccgccgcc acgacgccga cgagggaggg agcacggtga accgcccggc
      181 cgatgtcgag tccgccctgc agcggttgaa gggatggcgt gaccgcgcgg tcgccggccg
      241 ccaacccggt gcggagacga tccgcgacat ccacctgagc atggtggccc gctcgggtcg
      301 tcggatcccg tcggcgatcg ccgaggtgtt gccgcagtcg ttgaaggggt tcgccggcca
      361 gatggcccgg gcgatggtcg accgacccgg cccgggcgcg cagcccggcc cggccggtcc
      421 gccggtgcag ggcggcccac ctccgacgca aggtggccgg ccgggggccc cgccgcacca
      481 ccatccccag cagggcgccc ccggccacgt ctcgaacgtc ggcggggcac gtgacgtgcc
      541 gctcggtggg gtccggctcg ggggagaccc ggcggagccg acaagatctg gcccggcgga
      601 gccgacccga tcggcaccac cggtctcggc ggcctccggt ccacggcccc cgggctcgga
      661 cggctggggc gggtacccgc cggcctccgg ctcgtcggca tccgtgccac cggactcggc
      721 agcgtcggcg tcctcggcat cggtgccgcc gtcggcgatg agccccgatg cggaggccgc
      781 tgtgcgtccc ggtgggccac cggcacgacc gggtacatca ggatcgggca cgtcaggatc
      841 gggcacatca gggtcggcgc cagcggcacc gccaccgaag gagaccgacg aggaacggca
      901 gcagcggctg gcggcggagt gggagaagtt cgccccgatg gatttctcgg agctcggtga
      961 cgagccgggt cggatccagg ccgctgccgc accgggcggt ggtgtccggc tgcggtggca
     1021 gccggtcgac gacgaccccg ggcacaccgt ctaccgcgtg gtgtccggcg acgactacgc
     1081 cccgtacgat ccgcagaacg ccgacttcgt ggagatcacc accgacaccg cgtgcgtgga
     1141 tgaccgggag ttccgtgcgg cgatccggca cctgcaggtg tggcgcaaca gcggggccac
     1201 cgaggccgag gcgtacgcca accagccggt gatgcacgcc cgggtgccgc tggtcgcacc
     1261 cgtcacgggg gtcgacctgc gcgaggacga gggccgcgtc atcgggcagt ggagcgtctt
     1321 ccccggcgtg aagcgggtgc aggtctatcg catcccgatc gaacgggccc agtccgggtt
     1381 cggcgacccg gcgttccgga tcctggccga cgacgccaac ctgggcgggt tcgtcgacca
     1441 cggcgccgac cgcgggaagc gctacgtcta ccagttcttc tgcgaggccg agcatgacgg
     1501 gctggtgcgg ttgtcggcgc cgacgacacg gcagctgctg gtctcggcgg tgctggagcc
     1561 ggtcaccgac ctggagatcg agctggacaa ctccgcgacg gatgcacagt tcgacctgac
     1621 ctggaccaac ccgccggccg ggcgggtggt ggtctaccgc accgagcacc caccgcgggc
     1681 cggggcggac gcggaggccc gcgccgagtc ggcactgccg cagatggggt tgcccgacga
     1741 cgcccgcctg gtgcacccgg tggtcgcgga gggggaccgg tcggcgatgc ggaccgtgcc
     1801 gtggccgcgc ggctgggtgc ggacctactt caccccggtg acggtgttgg acgggcgcgt
     1861 gcaggtcggc aaccacatcc ctgcggtccg gacgatgccg gtgaccgatc cggttgtggt
     1921 ggagcgcacc cacacccagg tgctgaagtt cggctggccg cggggtgcct cgtacgtgtc
     1981 ggtcttcctg ggggcccggg accagccggc cgaggaactg ctggagaccg cgcagcggat
     2041 ggacatctcg gccgacgagt acacccggct gggagggatg cacttctccc gcacgctgcc
     2101 gagctccggt tgctcggtgc acatggtcgg ggtctcctac agcaacggtg agcggatcga
     2161 gagcgcgccg gtgtcggtgg actacccggg cctgctgcgg ctggaataca ccgtggagca
     2221 tcggaggggt cggctgaacc ggggcccgat gaccctgcag ctgcggatcc ggtccgagct
     2281 gaagctgacc cacgccccgc cgttcctggt ggtgttcaac ccggaccggt tcccgttgga
     2341 gtccggggac ggggacccgg tcgacgtggt gctcgacgac ggccgggcga acatgccggt
     2401 gcgccgattc gtccccgacg ggctcgaccc cgagtggtcg cagcagctgt ggcaggcaat
     2461 gcctccgggg ccgggcttcg tccggttgtt cctcgacctg ccgccggagc ggctgcagac
     2521 ggtcgcgctg ctggatcccg acatcaacca actgatcgtc cgagggtgac gccgatgtcc
     2581 caaccaccac agggcggtcc gccccagca
//
LOCUS       DATGUK010000015         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_17326,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000015 DATGUK010000000
VERSION     DATGUK010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17326"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VK103_00220"
     CDS             <1..999
                     /locus_tag="VK103_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010268616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLQ81931.1"
                     /translation="YPFSTTDFHGMPYRRFGTSGLQTSAMGLGTWKFGHPETGDGSRS
                     DEATSLAIMDRAYELGVTFWDTANRYNGGTGNSERVIGRWFDANPEKRRDIVLATKVF
                     GGMDGYTPNHSGLSRLQIIEGVKACLARMGQEWIDVLWFHGFDDNVPVEESLEAVEDL
                     VSQGLVHYLGVSQNSVQNLEAYLAASERISRRCRPVAVQNQYDPVNGADRPGVLEFCA
                     AEGLSFVPYSPLGQGLLTDRYLDPSKVGKGDRLHDQGMEIPEDKLARVRRVGELGEQW
                     GHSISQLTLAHLLSLDGMGTQIPSSSTVAQLEANAAAGTIELSDDQQAQLAEAFAG"
     gene            complement(1118..>2466)
                     /locus_tag="VK103_00225"
     CDS             complement(1118..>2466)
                     /locus_tag="VK103_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HLQ81932.1"
                     /translation="RIAHITDPHIRLHVPGSAVIPTRRSREAAELFRTAVADAVRRGA
                     EVLAVTGDLVDVPGHLFDIERDPAGDAELWQQVRRDYRWVAEVLDDSGLPWIALPGNH
                     DAYRVMREEFGPRPYVVDHGGVRFISFWDRETADHTPQRILTDRRRFESALRDDATPQ
                     VHLQHFVITPELNARYPHTYREGEQLRQRLAEADRTTLVLSGHYHRGTEPEQHGRAWF
                     SVTPALTEAPHPYRIFDLRPDADGRGASVTWEQVELDPEPSLAPVVFLDRDGCINTLA
                     AYHSGPEAMELIPGAAAAIRRLREAGWRVVVITNQACVGLGYVTTATLAEIHDRMAAL
                     LAAEGARVDAIYSSLEAGPDGITEEYRTNLTRKPLPTLLHRAVTDLGLDLTGGYMVGD
                     RQGDVDTAIAAGVTPLLVRTGNGRLTEQAWGADGDTATRCTVVDHLAAAATEILRA"
BASE COUNT          355 a          857 c          892 g          362 t
ORIGIN      
        1 tatcccttca gcacaacaga tttccacggc atgccgtacc gccggttcgg caccagtggc
       61 ctgcagacct cggcgatggg actgggcacc tggaagttcg gtcaccccga gaccggggac
      121 ggctcccgca gtgacgaggc cacctcgttg gcgatcatgg accgggcgta cgagctcggc
      181 gtcaccttct gggacaccgc caatcgctac aacggcggca ccggcaactc cgagcgggtg
      241 atcggccgct ggttcgacgc caacccggag aagcggcgcg acatcgtgct ggcgaccaag
      301 gtgttcggcg gcatggacgg gtacaccccg aaccactccg ggctctcccg gctgcagatc
      361 atcgagggcg tgaaggcctg cctggcccgg atgggccagg aatggatcga cgtgctgtgg
      421 ttccacggat tcgacgacaa cgtcccggtg gaggagtcct tggaggcggt cgaggacctg
      481 gtgagccagg gcctggtgca ctacctcggc gtctcgcaga acagcgtgca gaacttggag
      541 gcctacctcg cggccagcga gaggatctcg cggcggtgcc gcccggtcgc ggtgcagaac
      601 cagtacgacc cggtcaacgg ggcggatcgt ccgggggtgc tggagttctg tgccgccgag
      661 ggcttgtcgt tcgtcccgta ctcaccgctc ggccaggggc tgctcaccga ccgctacctc
      721 gatccgtcga aggtcggcaa gggtgatcgg ctgcatgacc agggcatgga gatcccggag
      781 gacaagctcg cccgggtgcg ccgggtcggt gagctcggtg agcagtgggg ccactccatc
      841 agccagctca ccctggccca cctgctgtcg ctggacggga tgggcaccca gatccccagc
      901 tcgtccaccg tcgcgcagct ggaggccaac gccgccgccg gcaccatcga gctcagcgac
      961 gaccagcagg cccagctcgc cgaggcgttc gccggctgac ggtccggccg atctcgagcc
     1021 gcaccggggc atcgctccgg gtcagcagat cgccccagcc cagcgcacga ccgaaggacc
     1081 gcaatggtcg atcccacgtg gtgtctggac gacccgctca ggctcgcagg atctcggtgg
     1141 ctgccgcggc caggtggtcc accacggtgc accgggtcgc ggtgtcgccg tcggcacccc
     1201 acgcctgctc ggtgagccgg ccgttgccgg tgcggaccag cagcggggtc accccggcgg
     1261 cgatcgcggt gtcgacgtca ccctggcggt ccccgaccat gtagccgccg gtgaggtcca
     1321 ggccgaggtc ggtcaccgcg cggtgcagca gcgtcgggag tggcttgcgg gtgaggttgg
     1381 tgcggtactc ctcggtgatc ccgtccggac cggcctccag gctggagtag atcgcgtcca
     1441 cccgggcccc ctcggcggcc agcagcgccg ccatccggtc gtggatctcg gccaacgtgg
     1501 cggtggtgac gtagccgagc ccgacacagg cctgattggt gatcaccacg acccgccacc
     1561 cggcctcgcg cagccgccgg atcgcagcgg ccgccccggg gatcagctcc atcgcctccg
     1621 ggccggagtg gtaggccgcc agggtgttga tgcagccgtc ccggtccagg aacaccaccg
     1681 gcgccaggct cggttccggg tcgagctcca cctgctccca ggtcacgctc gccccgcgcc
     1741 cgtcggcgtc gggccgcagg tcgaagatcc ggtacgggtg gggtgcctcg gtcagggctg
     1801 gggtgacgct gaaccacgcc cggccgtgct gttcgggctc ggtgccgcgg tggtagtgcc
     1861 cggacagcac caacgtggtc cggtccgcct cggcgagccg ctgccgcagc tgttcgccct
     1921 cccggtaggt gtgcgggtac cgagcgttca gctcgggagt gatcacgaag tgctgcaggt
     1981 gcacctgcgg agtggcgtcg tcgcgcagcg ccgactcgaa ccggcgccgg tcggtgagga
     2041 tgcgttgcgg ggtgtggtcg gcggtctcgc ggtcccagaa gctgatgaac cgcacaccgc
     2101 cgtggtcgac gacgtacgga cgcggcccga actcctcccg catcacccgg tacgcgtcgt
     2161 ggttcccggg cagcgcgatc cacggcaggc cgctgtcatc gagcacctcg gcgacccaac
     2221 ggtagtcgcg ccggacctgc tgccacagct cggcatcgcc ggccgggtcc cgctcgatgt
     2281 cgaacaggtg gccggggacg tcgaccaggt cgccggtgac ggccagcacc tcggcgccgc
     2341 gccggaccgc gtcggcgacg gcggtgcgga acagctcggc cgcctcccgg ctgcgccggg
     2401 tggggatcac cgccgagccc gggacgtgga gtcggatgtg gggatcggtg atgtgggcga
     2461 tgcgca
//
LOCUS       DATGUK010000016         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1180238,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000016 DATGUK010000000
VERSION     DATGUK010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180238"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(521..1204)
                     /locus_tag="VK103_00230"
     CDS             complement(521..1204)
                     /locus_tag="VK103_00230"
                     /inference="COORDINATES: protein motif:HMM:NF033748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HLQ81933.1"
                     /translation="MSHQDRRRFFRTLMGLTTLGAVVGCAPRGEAVRAPGTTASPPGR
                     AHSSRSSSPATAIPQSPEEIRPARSAATERSTPSSPPVSLGIPDIGVSAEVMRLDLQP
                     DGSMEVPPFDRADEAGWYIESPAPGQIGPVVIVGHIDAPGGPAVFYRLADLRPGQSVH
                     LTCDDGRKVTYRAERIQLFPKEEFPTHLVYGDLDYPGIRLITCGGAYQRAAGGYQGNI
                     VVFGRIHDS"
     gene            complement(1201..1863)
                     /locus_tag="VK103_00235"
     CDS             complement(1201..1863)
                     /locus_tag="VK103_00235"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HLQ81934.1"
                     /translation="MKQRRNRRRGFTGQGRRGIIAAATLAAGLALVAPLLVGLTDQGT
                     AGAAPEDSVAPTAARTTPLRAPEPIVLGRSEPESITIPAIGVGTDLVALPKEKGKDTI
                     QLPKDPRQPGWFEESATPGELGITTIIGYIAKSNKEPGVFSKIAKLEEGDTITVTRAD
                     GRAVEFTVDSIDFYPEGEFPADEVYEDPDRPALRLITSGGTIHPDDPPGNLVVSARMT
                     GS"
BASE COUNT          298 a          699 c          731 g          372 t
ORIGIN      
        1 gagcgtgtca tggcccctgc tggccgtcga cgccgggatc gccgtgggtg ccgcattgtt
       61 ggcctcggtc ctgccatcgc gacgggccgc gctggccgca ccgaccgagg cgctcgccga
      121 agcctgacgt cgactcgggg agcccaggca ggcgactgcc ccgtacgccg accacctgtc
      181 ggacgtacgg ggcagtcctg gagggcggca ccggtggtgc accctgccgg gatgtcggac
      241 cgagcccgct gtgcgggcgc tccgtcatcc ccgaccccgg acaacagccc atcgtccgac
      301 gacgtggacc gggcggtccc acaccatggc ctgccctgcc cgcccctttc ggcacggcag
      361 gaggtgacac cgttgctgtc cgcgagtcac gcggttcgtc ggtggccacc ggcggcatcg
      421 ggtcgatgcg ccccatggtc gacatcccca actgggacgg ggtccggctg tccggagttg
      481 gcctcagccc ctcgtcacgg tcatggcgct ccttctgggg tcaggagtca tggatgcgcc
      541 cgaacaccac gatgttgccc tgatagccac ctgctgcgcg ttggtacgcg ccgccacagg
      601 tgatgagccg gatcccgggg tagtcgaggt cgccgtagac gaggtgcgtg gggaactcct
      661 ccttcgggaa cagttggatc cgctcggccc gataggtgac cttgcgtccg tcgtcacagg
      721 tgagatggac cgattgaccg ggtcgcaggt cggccaaccg gtagaacacg gctggcccgc
      781 cgggggcgtc gatgtgtccg acgatgacca ccggtccgat ctgtcccggc gcgggcgact
      841 cgatgtacca accggcctcg tctgcccggt cgaagggtgg aacctccatg ctcccgtccg
      901 gttgcaggtc gaggcgcatg acctctgctg acacaccgat gtcggggatg cccagtgaga
      961 cgggcgggct tgacggtgtc gagcgctcgg tggctgccga ccgtgccggc cggatctcct
     1021 ctggagactg ggggatcgcc gttgctggcg aggacgaccg tgaggagtgg gctcgcccgg
     1081 gcggtgaggc agtcgtgccg ggagcacgaa ctgcttcacc gcgcggggca cagccaacga
     1141 cggcgccgag ggtggtcaat cccatcagcg tccggaagaa gcgccgcctg tcctggtgac
     1201 tcatgaccct gtcatgcgtg cgctgacgac caagtttccg ggcggatcgt ccgggtggat
     1261 ggtcccacca ctggtgatca gccgcagagc cggtcggtcc ggatcttcgt aaacctcgtc
     1321 ggcagggaac tcgccttctg ggtagaagtc gattgagtcc acggtgaact cgactgcgcg
     1381 cccgtcggcg cgcgtcacgg tgatcgtgtc accttcttcg agcttggcga tcttgctgaa
     1441 cacgcccggt tccttgttgg acttggcgat gtaaccgatg atcgtggtga tccccagttc
     1501 gcctggtgtc gccgattctt cgaaccaccc gggctggcgg gggtccttgg gcagctggat
     1561 cgtgtccttg cccttctcct tgggaagtgc gaccaggtct gtgccgaccc caatggccgg
     1621 gatcgtgatc gactccggct ccgagcggcc gagcacaatc ggctcgggag ctcggagagg
     1681 ggtcgtgcgt gctgcggtcg gggccaccga gtcctccggt gctgcgccgg ccgtcccttg
     1741 gtcagtcagg cccaccagca gtggggccac cagtgccagg ccggcggcca gggtcgccgc
     1801 ggcgatgatg ccgcgacgac cctgaccggt aaagccccgg cggcgattgc gccgttgctt
     1861 catcgattcc tccttgcatt tgccatccgg ctcgtcctgc gggtgagcag ggcgagtgcg
     1921 accatgccgg ctgtgatgat gaccaattgg ccctcacctg tgcggtcggt cattgcggtt
     1981 gacccgaaac cggcgtcaac gcctcccttg ggggtacgac cgacctgacc accgacgtgc
     2041 tggcggccac cagcctcggg tgcggatcca cccggattgc gcccggtcga tgcaccgacg
//
LOCUS       DATGUK010000017         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1526736,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000017 DATGUK010000000
VERSION     DATGUK010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526736"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VK103_00240"
     CDS             <1..230
                     /locus_tag="VK103_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLQ81935.1"
                     /translation="IPGLGLYFATAAQSRDMPLLMGSTLFFAIILVLMNLLVDLSYRI
                     LDPRVRYQTPAQTARSRRRRRTQLRKVAANV"
     gene            223..1236
                     /locus_tag="VK103_00245"
     CDS             223..1236
                     /locus_tag="VK103_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLQ81936.1"
                     /translation="MSDDVTAGRDSNTRPTGPSPIGSGPAEPDTGLPRSADPVEPATT
                     DGPPTGGSDRARSEFARAWARYRHNKVAFASLVFVGLLVLTAIFGPFFTPYGVSEQDI
                     TNRLQPMGSPGHLLGTDALGRDLLSRLVHSLRSALTIGFVAEGLSLGFALLIGMTAGF
                     LGGRAEQLLMLFTDVMFAFPRYLFAIILVVAMGQGLWTIIIAISVASWVGQARLARAQ
                     ALKIKNFAYVEAGRSMGARGFTLVLRYILPNSWGPLLVTTSFGIPAAIAAEAGLAILG
                     LGVQPPTPTWGGMIMDGYHYVLGVPHLIVWPLLLFSLTMLAFTFIGDGFRDAFDVAEE
                     EQR"
     gene            1233..>2088
                     /locus_tag="VK103_00250"
     CDS             1233..>2088
                     /locus_tag="VK103_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLQ81937.1"
                     /translation="MTVTGGRTTARTGAASTTRAPRPEDQGVDARPTESPLLTVRDLR
                     VVFRDRRGRENVAVDGFSADVTRGKHLAVVGESGSGKTVSMRALLHLLPGTARVTGQA
                     VFDGEELLDRRPERMQSVRGRRIGMVFQNPAQAFNPTLTLKRQLTEHLLWHRMCGPKE
                     AVERAVEALDRVGIPDPARRIKLYPFQLSGGMLQRAMVAQAIVAQPEILIADEPTTAV
                     DVTLQRQVLDLLLSLGDDGLSMVVITHDLGVARYMCSDVIVMQNGSIVERGEMATFAR
                     DARTDYARR"
BASE COUNT          308 a          740 c          706 g          334 t
ORIGIN      
        1 ggatcccggg actcgggctc tacttcgcca ccgccgccca gagccgggac atgccgctgc
       61 tgatggggtc cacgttgttc ttcgccatca tcctggtgct gatgaacctg ctggtcgacc
      121 tcagctaccg gatcctcgac ccgcgggtgc gctaccagac cccggcacag acggcacgca
      181 gtcgccgacg tcgccggacc cagctgagga aggtggccgc caatgtctga cgacgtgacc
      241 gcgggccgcg acagcaacac ccggccgacc ggaccgtccc cgatcgggtc cggcccggcc
      301 gagcccgaca ccggcctgcc ccggtccgcc gaccccgttg agccggccac caccgacggc
      361 ccgcccaccg gcggctccga ccgggcccgc agcgagttcg cccgggcgtg ggcgcgctac
      421 cgccacaaca aggtcgcctt cgccagcctg gtcttcgtcg gcctgctggt gctgaccgcc
      481 atcttcgggc ccttcttcac cccgtacggc gtgagtgagc aggacatcac caaccggctg
      541 cagccgatgg gctcccccgg ccacctgctc ggcaccgacg cactcggccg cgacctgctg
      601 tcgcggctgg tgcacagcct gcgcagtgcg ctcacgatcg gtttcgtggc cgagggattg
      661 tccttggggt tcgcactgct gatcgggatg acggccggct tcctgggtgg ccgcgccgag
      721 cagttgttga tgctgttcac cgacgtgatg ttcgccttcc cccgctacct gttcgcgatc
      781 atcctggtgg tggcgatggg acagggactg tggacgatca tcatcgcgat ctcggtggcg
      841 tcgtgggtgg ggcaggcccg gctcgcccgc gcccaggcac tcaagatcaa gaacttcgcg
      901 tacgtggagg caggccgttc gatgggggct cgcgggttca ccctcgtgct gcgctacatc
      961 ctgcccaatt catgggggcc gctgctggtc accaccagtt tcggcatccc ggccgcgatc
     1021 gcggccgagg ccggtctggc catcctgggc ctcggcgtac agccacccac gccgacctgg
     1081 ggtggcatga tcatggatgg ttaccactac gtgctgggcg ttccgcacct gatcgtgtgg
     1141 ccactgctgc tgttctcgct caccatgctg gccttcacat tcatcggaga cgggttccgg
     1201 gacgccttcg acgtggccga ggaggagcaa cgatgacggt gaccggggga cgcaccaccg
     1261 cccgcacggg ggcggcgagc acgacgaggg cgccccgtcc ggaggaccag ggcgtcgacg
     1321 cccggccgac ggagagcccg ttgctcacgg tgcgcgacct gcgggtggtg ttccgggacc
     1381 gccggggcag ggagaacgtc gccgtcgacg gattcagcgc cgacgtcacc cggggcaagc
     1441 acctcgccgt ggtcggcgag tccggctccg gcaagacggt cagcatgcgt gcgttgctgc
     1501 acctgttgcc cggcaccgcc cgggtgaccg ggcaggcggt cttcgacggc gaggaactcc
     1561 tcgacaggcg cccggaacgg atgcagtcgg tccgtggtcg tcgcatcggg atggtgttcc
     1621 agaacccggc ccaagcgttc aacccgaccc tgacgctcaa gcggcaactc accgaacacc
     1681 tgctgtggca ccggatgtgc gggccgaagg aggccgtgga gcgggccgtg gaggcactgg
     1741 accgcgtggg gatcccggac ccggccagac gcatcaagct ctatccgttc cagttgtccg
     1801 gaggcatgct gcagcgcgcc atggtcgccc aggccatcgt cgcccagccg gagatcctga
     1861 tcgccgacga gcccaccacc gcggtcgacg tcaccctgca acgacaggtg ctcgacctgc
     1921 tgctgagcct cggcgacgac gggctgtcga tggtggtcat cacccacgac ctcggtgtcg
     1981 cccggtacat gtgctccgac gtgatcgtga tgcagaacgg cagcatcgtc gaacgtggtg
     2041 agatggccac cttcgcccgg gacgcgcgca ccgactatgc ccgtcggc
//
LOCUS       DATGUK010000018         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1299120,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000018 DATGUK010000000
VERSION     DATGUK010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299120"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VK103_00255"
     CDS             complement(<1..601)
                     /locus_tag="VK103_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLQ81938.1"
                     /translation="MTPVIRLTDVAVEFRRTTLLTEVNLSVEEGDCCAIVGPNGSGKT
                     VLLKIMCGFLAPTRGTVSISPEYLSRRRTFPERFGIILDRPGFLAGQSGMDNLRALAA
                     IRGVIGDDEIVAALDAVDLDPTLRQPVGRYSMGMKQKLALAQAIMEDQTVLILDEPFN
                     ALDEDSVEIVREVLSDRLEAGCTLVFTSHQQADLDALDPR"
     gene            complement(598..1935)
                     /locus_tag="VK103_00260"
     CDS             complement(598..1935)
                     /locus_tag="VK103_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81939.1"
                     /translation="MRLLRGIILVVTAVVSIGYGRYVVGAGSDPLHGVRGPYLIGYGI
                     APLICVLAASDAWRAARDVVLVRSGSVSRWWRDRVGAGATAGGQLALAMVGGTMVARV
                     GLPVPSVGEVVASVLAPVLVVLGITMISHGVAALTALIGRTALVVAVVAWWVAILVAG
                     RRAASLSSWANPSRLMTHPVDAVHLVWPVLMMAAIGHVVDLVRQGRWQVIVRPALVYG
                     AVGLACLGAQATSGTPVVQADGGVLAEADLIPQTLNGVGAGGGVRQLLFVIILWLGHP
                     AAFAADLAMHRHGSWHQELIRVGSVGRWYWRRCWPWLLSAPVVPLVSLVVAQLAVWAL
                     GSATPLSAGDALHVAATGTLQLIVYLLVMLVVQLLHGGPAGTIIALVGLSALTFVRHA
                     GVPVPAIAMNGLPEVAAGGTLGTCAVLAGAAAALAAGVAILARHIPPDWSSPS"
     gene            complement(1932..>2625)
                     /locus_tag="VK103_00265"
     CDS             complement(1932..>2625)
                     /locus_tag="VK103_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81940.1"
                     /translation="WTLGVADHYVMGHQIEFVQLVLRSTPVALVFPALAVLPFTVVAV
                     SATRNNFLLYTRTRRRIRSTLVRLYIGAAVVTFAVFAIIMLTYELGAMVSEVAGTVDY
                     VPGRVDRTPETTTTFSQLMVYGTGAYYAFQCVWFGVNAAAYAVLGLSCVLVIRQPVVA
                     LLLPWLVFVVVGFGMAVLGLEAFSPFTVAPANLTQLPMWMPMVPAAVLVLVAVGSALG
                     TVWYADRLAVTA"
BASE COUNT          482 a          941 c          874 g          328 t
ORIGIN      
        1 gccgggggtc gagcgcgtcg aggtcggcct gctggtggct ggtgaacacc aacgtgcatc
       61 ccgcctcgag ccggtccgac agcacctccc gcacgatctc gacgctgtcc tcgtcgagcg
      121 cgttgaacgg ctcgtcaagg atgaggaccg tctggtcctc catgatcgcc tgggccaggg
      181 caagcttctg cttcatcccc atcgagtagc gtccgacggg ctgacgcagg gtcgggtcga
      241 ggtcgaccgc gtccagggcg gcgacgatct cgtcgtcccc gatcaccccg cggatcgcgg
      301 ccagggcgcg cagattgtcc atgccgctct gaccggccag gaatccgggc cggtccagga
      361 tgatgccgaa ccgctcgggg aaggtgcggc gacgcgacag gtactccggg ctgatcgaga
      421 cggtgccgcg ggtgggtgcg aggaagccac acatgatctt gagcagcacg gtcttgccgg
      481 agccgttcgg cccgacgatg gcacagcagt ccccttcctc gacggacagg ttgacctcgg
      541 tcagcagggt ggtgcgacgg aactcgacgg ccacgtcggt cagtcggatc actggggtca
      601 tgacggggaa ctccaatcgg gagggatgtg tcgagccagg atggcgacac cggctgccag
      661 cgccgccgct gcgcccgcga gcacggcgca ggtgccgagc gtgccgcccg ccgcgacctc
      721 ggggagcccg ttcatggcga tcgcgggcac cggtacgccg gcgtggcgta cgaaggtcag
      781 tgcggacagc ccgaccaggg cgatgatggt gccggcgggg ccgccgtgca acaactgcac
      841 gaccagcatc accaacaggt acacgatgag ctgcagggtg ccagtggcgg cgacgtgcag
      901 cgcgtctccg gcggacaggg gcgtcgccga tcccaacgcc cacacggcga gctgcgcgac
      961 caccaggctg accagcggga ccaccggagc gctgagcagc cagggccagc accgtcgcca
     1021 gtaccaccgc ccgacgctgc cgacacggat cagttcctgg tgccagctgc cgtggcggtg
     1081 catggccagg tcagcggcga acgctgcagg atgcccgagc cagaggatga tcacgaacaa
     1141 cagctgtcgg acaccgcctc cggcgccgac gccgttcaac gtctgcggga tcaggtcggc
     1201 ctccgccagc acgccaccgt ccgcctggac caccggcgtg ccgctggtcg cctgggcgcc
     1261 caggcaggcc agcccgaccg cgccgtacac cagtgcgggg cggacgatga cctgccatcg
     1321 tccctgccgc accagatcga ccacgtgacc gatcgctgcc atcatcagga cgggccacac
     1381 caggtgcacg gcatccacgg gatgggtcat cagtcgggac ggatttgccc agctcgacaa
     1441 ggatgcggcc cgtcgtcccg ccaccaggat cgcgacccac cacgccacca ccgcgacgac
     1501 cagggccgtc cgcccgatca gcgcggtgag cgcagccaca ccgtgactga tcatggtgat
     1561 gccgaggacc acgagcaccg gcgccagcac actcgcgacg acctccccca ccgaggggac
     1621 cggcagcccg acccgtgcca ccatcgtccc gcccaccatg gccagggcga gctgtcctcc
     1681 ggcggttgcg ccggccccca ctcggtcacg ccaccagcgg ctgaccgacc cactgcgcac
     1741 cagcacgacg tcgcgcgccg cacgccatgc gtcgctggcc gcgaggacac agatcagcgg
     1801 agcgatcccg tacccgatca ggtacgggcc gcgcacaccg tgcaacgggt ccgagccggc
     1861 cccgacgacg taccgtccgt atccgatgct gacgacagcg gtcaccacga ggatgattcc
     1921 gcgcagcagt ctcatgcggt gaccgcgagc cggtcggcgt accagacggt gcccagcgcc
     1981 gacccaacgg ccacgagcac caggacggcc gccggcacca tcggcatcca catcggaagt
     2041 tgggtcaggt tggccggcgc aacggtgaac ggggagaacg cctccagtcc gagcacggcc
     2101 atgccgaacc cgaccacgac gaacaccaac cacggcagca acagcgccac caccggttgc
     2161 cggatcacca gcacacaact gaggccgagc acggcgtagg ctgcggcgtt gacgccgaac
     2221 cacacgcatt ggaaggcgta gtaggcaccg gtgccgtaca ccatcagttg cgagaaggtg
     2281 gtggtggtct ccggagtcct gtccacccga cccggaacgt agtccacggt cccggccacc
     2341 tcgctgacca tcgcccccaa ctcgtaggtg agcatgatga tcgcaaacac ggcgaacgtc
     2401 acgacggcgg cgccgatgta cagccggacc agcgtcgatc tgatccggcg tcgggtccgg
     2461 gtgtacaaca ggaagttgtt cctggtcgcg gacaccgcca ccaccgtgaa cggcagcacc
     2521 gccagggccg ggaagaccag cgcgaccggt gtggagcgca gcaccagctg gacgaactcg
     2581 atctggtgac ccatcacgta gtggtccgcg acaccgaggg tccac
//
LOCUS       DATGUK010000019         4023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_951083,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000019 DATGUK010000000
VERSION     DATGUK010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951083"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VK103_00270"
     CDS             <1..1083
                     /locus_tag="VK103_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLQ81941.1"
                     /translation="DDSLSGTPGSTVSLATRPPVVIPPTPGETTAVPRDDPSTAGPAA
                     PRETGPRPVVFKAEPTRGTDLQVTLDLAMQRKAERILASQKSPTAMAVLDPDTGAALV
                     LAVSDSAEGRPLATTGRYAPGSTFKVVTSLALLRQGLTPESMVNCPQRTVVDGRVIEN
                     YDDFPRSRVGRMRLLDALALSCNTAFVNERDKLSGPRIRGAAVSLGMGQDHDAGFPSF
                     FGSVPDPANVVGLAEASFGQGTVQASPMSMAAVAASVSAGRTRVPYLISDNRPEQDGK
                     PLTAGEAKDLQAMMGAVVKSGSGRSLGKLVTGAKTGTAEYGTKKPPKTHAWMMAYTSD
                     LAIAVLVTDGRSGSGSAGPLIKQFLS"
     gene            complement(1140..1766)
                     /locus_tag="VK103_00275"
     CDS             complement(1140..1766)
                     /locus_tag="VK103_00275"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HLQ81942.1"
                     /translation="MSAPGGTVVTRELRRQTVVRSLPPIVLAALMWVSEIIDTVPGVD
                     LDQYGIQPREVDGLTGIAAAPMLHAGFDHLVANTSVFVVLGLLVAWLTKHFWKVTVGV
                     ALLGGLAVWLVGAPQSVHIGASGLVYGYAAFLVVYGLVARRVTAVIVAVVVFLVYGSI
                     VWGVLPGQPGVSWEGHLFGAVAGLLLAVLLGRRDRRRARQAAVGSGPA"
     gene            1940..2494
                     /locus_tag="VK103_00280"
     CDS             1940..2494
                     /locus_tag="VK103_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81943.1"
                     /translation="MSEQNPWDFSEYSESADSSGPANGPGRPTSGPFQPASTPTPQQP
                     GGFGVAPGPAPFGAASLDSGQSPTTMTAAFGGTAEPPRHLSTSSPPVLWLGIAALLAV
                     AGLVLALLLGELPPVAIGAWALAGPIGIGMLAVFTTRDIRARSGAVYLAQGWVRPLYW
                     VCAGLCLAGAMVAAWQIAEWVGRL"
     gene            2584..>4023
                     /locus_tag="VK103_00285"
     CDS             2584..>4023
                     /locus_tag="VK103_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLQ81944.1"
                     /translation="MLLAVVAALALAGSARTAHAEEESAVDVYGGCLVAQKAGDLLII
                     VDESASLDTNDPDNARIASAKYLLTQLNAFADRAKVTLDVSVAGFSHEYSEVQDWTKL
                     DDAGLQQTLGTVDSFADRPYGFETDYWMALEGARRSLAEHNPEGDRCQALVWITDGGL
                     DVDFRRDGQQRRDYGETKPYLPDTKLADEDAAQQAEAEAERQICRNGGLADQLRSSKV
                     TVFGVGLRGAEKPDYTLMKSIALGGDAGGQHCGKITRPPGDFTEAANIDQLLFEFDKF
                     ATPNQKKRTTESGVCQGEPCPEERHTFVLDDSVQSVHVLGSSDVDDAEVYLVTPAGKE
                     VKLTRKGIGETTGADTGAVSIDYSWQSERTLVIDLAAEAGTQGWAGPWSVVFVDPTGK
                     SPDGKSRTSIAISGDVFPAWPDVDSTRLRAGEPATVQLGLVNADGDPIDPAKLLGTVA
                     MDVTLETADGRTVQVADGLGKTGIAGPVTL"
BASE COUNT          623 a         1464 c         1402 g          534 t
ORIGIN      
        1 gacgactccc tcagcggcac cccgggcagc accgtctcgc tggcgacccg gccaccggtg
       61 gtgatcccgc cgaccccggg ggagactacg gcggtcccgc gggacgaccc gtccaccgcc
      121 ggccccgctg cccctcggga gaccggcccc cgcccggtgg tgttcaaggc cgagccgacc
      181 cgtggcaccg acctgcaggt caccctcgac ctggcgatgc agcgcaaggc ggaacggatc
      241 cttgcatccc agaagtcacc gaccgcaatg gcggtgttgg accccgacac cggggccgcg
      301 ctggtgctcg cggtgagcga cagtgccgag ggccggccac tggcgacgac gggccgctac
      361 gcaccaggtt cgacgttcaa ggtcgtcacc tcgctggcgc tgctccggca ggggttgacg
      421 ccggagtcga tggtcaactg cccgcagcgc acggtggtgg acgggcgcgt gatcgagaac
      481 tacgacgact tcccacgcag ccgggtcggc cggatgcggt tgttggacgc gctcgcgctg
      541 tcgtgcaaca ccgccttcgt caacgaacgc gacaaactgt ccggaccgcg catccggggg
      601 gctgcggtca gcctcgggat gggacaggac cacgacgccg ggttcccgtc cttcttcgga
      661 tcggtgcccg atccggccaa cgtcgtcggg ctcgcggagg ccagcttcgg gcaggggacg
      721 gtgcaggcct cgccgatgtc gatggccgca gtcgccgcct cggtgtcggc cgggcgcacc
      781 agggtcccgt acctgatctc ggacaaccgg cccgagcagg acggcaagcc cctcaccgca
      841 ggcgaggcga aggatctgca ggccatgatg ggggccgtgg tgaagtccgg ctccggtcgc
      901 tccctgggca agctggtgac cggggcgaag accggcaccg ccgagtacgg gaccaagaag
      961 cctccgaaga cgcatgcctg gatgatggcc tacacctccg atctggcgat cgcggtgctg
     1021 gtgaccgacg gccggtccgg ctccggcagc gccgggccgt tgatcaagca gttcctgtcc
     1081 tgacccgggc cgcatcgatc ctgcagcccg tcccaccccg gatcgccccg accgatcggt
     1141 caggcaggcc ctgacccgac ggccgcctgc cgggcacgcc gtcggtcccg ccggcccagc
     1201 agcaccgcca gcagcagccc ggccaccgct ccgaacaggt ggccctccca ggacaccccg
     1261 ggctggccgg gcagcacccc ccacacgatg ctgccgtaca ccaggaacac caccacggcc
     1321 acgatcaccg cggtcacccg gcgcgccacc agcccgtaca ccaccaggaa ggcggcgtag
     1381 ccgtacacca ggccactggc gccgatgtgc acgctctgcg gcgcgcccac cagccacacc
     1441 gcgagcccgc ccagcagggc caccccgaca gtgaccttcc agaagtgctt ggtgagccac
     1501 gcgaccaaca accccagtac gacgaagacc gaggtgttgg cgaccaggtg gtcgaagccg
     1561 gcgtgcagca tcggcgcggc cgcgatcccg gtgagcccat ccacctcgcg cggctggatc
     1621 ccgtactggt ccaggtcgac gccgggcacg gtgtcgatga tctcgctgac ccacatcagc
     1681 gcggccagca cgatcggtgg caagctgcgc accaccgtct gccgacgcag ctcccgggtc
     1741 accaccgttc cgcccggggc gctcaccgtg ccgctccagt actcacgact ccaccgtacg
     1801 gggagcgggc aacgccggcg gcgggaccgg tggaccgtgc gggacggggg ccggtcacga
     1861 catcggcagg gctgccccgc cgcgctccta tacttgcggc agtttgccga cgcccctccc
     1921 gccatcggag aagaccgaga tgtccgaaca gaatccatgg gacttcagcg agtacagcga
     1981 gtccgcggat tcgtcgggcc cggccaatgg cccgggtcgt cccaccagtg ggccgttcca
     2041 gccggccagc accccgaccc cgcagcagcc cggcggcttc ggggtcgcgc cgggaccggc
     2101 gccgttcggg gccgcgagcc tcgacagcgg gcagtccccg acgacgatga ccgctgcgtt
     2161 cggcggcact gccgagccgc cccggcacct gtccacgtcc tcgcccccgg tgctgtggtt
     2221 gggcatcgcc gctctgctgg cggtcgccgg cctggtgctg gcgctgctgc tcggtgagct
     2281 gccgccggtt gcgatcggcg cctgggcgct cgccgggccg atcgggatcg gcatgctggc
     2341 ggtgttcacc acccgcgaca tccgggcccg gtccggcgcg gtctacctgg cgcagggctg
     2401 ggtgcggccc ctctactggg tctgcgcggg cctgtgcctg gccggggcga tggtcgctgc
     2461 ctggcagatc gcggaatggg tgggtcgact gtgaggactg tcgagaacgg gcgacctgcc
     2521 acgggcccga ggacggtcgc tcgccggcgt cgggatgcga cccgccgcgc ggcgatctgg
     2581 acggtgctgt tggccgtggt cgccgcactg gcgctggccg gcagtgcccg gaccgcacac
     2641 gccgaggagg aatcagcggt cgatgtgtac ggcggctgcc tggtcgccca gaaggccggc
     2701 gacctgttga tcatcgtcga cgagtcggcg agcctggaca ccaatgaccc cgacaacgcc
     2761 cggatcgcgt ccgccaagta cctgttgacc cagctcaacg cgttcgccga tcgcgccaag
     2821 gtgaccctgg acgtcagcgt tgccgggttc tcccacgagt actccgaggt gcaggactgg
     2881 accaagctcg acgacgccgg gctgcagcag accctcggca ccgtcgacag cttcgccgac
     2941 cgcccgtacg gcttcgagac cgactactgg atggcgctgg agggggcccg ccggtcgttg
     3001 gccgagcaca atccggaggg ggaccggtgc caggccctgg tgtggatcac cgacggcggc
     3061 ctcgacgtgg acttccgccg cgacggacag cagaggcgcg actacggcga gaccaagccg
     3121 tacctgcccg acaccaagct cgccgacgag gacgccgcac agcaggcgga ggcggaggcg
     3181 gaacggcaga tctgccgcaa cggcgggctg gccgaccagt tgcggtcgtc gaaggtgacg
     3241 gtgttcggtg tcgggctgcg tggtgcggag aaacctgact acaccctgat gaagtcgatt
     3301 gcgctcggcg gggacgccgg tggccagcac tgcgggaaga tcacccggcc cccgggggac
     3361 ttcaccgagg cagccaacat cgaccagttg ctgttcgagt tcgacaagtt cgccaccccg
     3421 aaccagaaga aacgcaccac cgagtccggt gtctgccagg gcgagccgtg cccggaggaa
     3481 cgccacacct tcgtgctgga cgactcggtg cagtcggtgc acgtgctcgg cagctccgac
     3541 gtcgacgacg ccgaggtcta cctggtcacc cctgccggca aggaggtcaa gctgacccgc
     3601 aagggcatcg gggagaccac cggcgccgac accggcgcgg tgtcgatcga ctactcgtgg
     3661 cagtccgaac ggaccctcgt catcgacctg gccgccgagg ccggcaccca gggctgggcc
     3721 ggtccctggt cagtggtgtt cgtcgacccg accggcaagt ccccggacgg caagtcccgt
     3781 acctcgatcg cgatctcggg cgatgtgttc ccggcctggc cggacgtcga ctccacccgg
     3841 ctccgtgccg gcgagccggc caccgtgcag ctgggcctgg tcaacgccga cggcgatccg
     3901 atcgacccgg ccaagctgct gggcacggtg gcgatggacg tcaccctgga gaccgctgac
     3961 ggccggaccg tgcaggtcgc cgacggactc ggcaagaccg gcatcgccgg gccggtgacc
     4021 ctg
//
LOCUS       DATGUK010000020         2846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_834532,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000020 DATGUK010000000
VERSION     DATGUK010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834532"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1871
                     /locus_tag="VK103_00290"
     CDS             <1..1871
                     /locus_tag="VK103_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HLQ81945.1"
                     /translation="ASEHTWLLHDIVRQVWGFDGVLVSDWGGTHSTVDAATAGLDLEM
                     PGPPTHFGPRLADAVHSGEVAPQVLDDKVRHILRLAHRTGAMTTDEAAELPTPTGARN
                     GLAREAASAAAVLLKNTDGTLPLDASRIRSLAVIGPCARTTHIQGGGSAKVNAPYAVS
                     VLDGLQDALGEDVRVEHAQGCSIDRFTPTITSENARTPDGDIGILVELLRNDTDQEQL
                     VRSEVVDEVDLAWIGAPLDEVPMADTTIRCRFSYRPEQSGDHVFGLVSAGRAVAEIDG
                     RPLLDSADTTGHGPHFFGRGSDELRATVNLDGDRWHDIVITVRTRTEKSATAGVVLGV
                     VPPQPVDPIEDAVALAGRADAAVVVVGTGPEWEMEGGDRPALELPGEQPELITRVAAA
                     NPRTVVVVNAGSAVDLAWEDDVPAVLYGWFGGQEVGNAVADVLLGRSEPGGRLPFSIP
                     ADPGDMLDIGYRGDGGPGWGGTGGTMSYREGDAIGHRHHARSGRRARHGFGHGESYTT
                     FSHDDFHVREEDGQLLARCTVRNVGDREGSTVPQVYVTAPGPHRTRRLVGFTKATLAA
                     GEETGVTMALDPRTVESWSVEESDWAPVPGTHVLELTLSAAAPALGRIDYTVRARD"
     gene            complement(1847..>2846)
                     /locus_tag="VK103_00295"
     CDS             complement(1847..>2846)
                     /locus_tag="VK103_00295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HLQ81946.1"
                     /translation="GLDSGCQVGVVGGKSFSAGFVSDPERSLAVPSYLADAVRNVVGA
                     DHVRNAQQLFVHPEVGLRSINTAHEVAEAEYASAISSLAVHRAAAAITVGERADRLAD
                     MLSDGGVQRSVHPMVNFGERTRLGVVSPIDQRVELGDAFQIAFGLKGSLTCRSGIVAH
                     GPGDLEPATAEFFEALTLNYFDTVVAWYRAVRIGATGGEVHAAADTVRDDSLFRFSLN
                     PGHTLGYEEWLESPFSPGNPTPLRSGLLLQGDIIPEYDGPTVGANIEDGVALADEELR
                     ASLAETYPEAYDRIQQRRDYLAETIGVQLDASVLPLSNTPLWHAPYVLDQSRALTV"
BASE COUNT          444 a          985 c         1002 g          415 t
ORIGIN      
        1 acgcctcgga gcacacctgg ctgctgcacg acatcgtccg acaggtttgg gggttcgacg
       61 gtgtgttggt ctccgactgg ggtggcaccc actccaccgt ggacgcagca acggcgggac
      121 tcgacctgga gatgcccggg ccgccgaccc acttcggacc acggttggcc gacgcagtcc
      181 actccggtga ggtcgcgcca caggtccttg acgacaaggt gcgacacatc ctgcgactgg
      241 cacaccgcac cggcgccatg acgaccgacg aggcagccga gctgccgact cccaccgggg
      301 cacggaacgg gctggcgcgc gaggcggcca gcgctgccgc cgtgctgctc aagaacaccg
      361 atgggacgct tccgctcgat gcctcccgga tccgctcact ggccgtgatc ggcccgtgcg
      421 cacgcaccac ccacatccag ggcggtggat cggccaaggt gaacgcgccg tacgcggtga
      481 gcgtgctcga cgggctgcag gacgcgctcg gggaggacgt acgtgtggaa cacgcgcagg
      541 gttgttcgat cgatcggttc accccgacga tcacctcgga gaacgcccgg acaccggatg
      601 gcgacatcgg gatcctggtc gagctccttc gcaacgacac cgaccaggag caactggtcc
      661 gttcagaggt cgtcgacgag gtcgacctgg cctggatcgg agcacccctg gacgaggtgc
      721 cgatggccga caccacgatc cgctgccggt tcagctaccg acccgagcag tcgggcgacc
      781 atgtcttcgg cctggtgagc gcgggccggg cggtggctga gatcgacggt cgaccgctcc
      841 tcgactcggc cgacaccacc ggacacggtc cccacttctt cggccgcggg agtgacgagc
      901 tccgcgccac ggtcaacctg gacggggacc gctggcacga catcgtgatc accgtgcgca
      961 cccggaccga gaagtccgcg accgccggtg tcgtgctcgg agtggtcccg ccccagcccg
     1021 tcgacccgat cgaggatgcg gttgcccttg ccgggcgggc ggacgcggcc gtcgtggtgg
     1081 tgggcaccgg tccggagtgg gagatggaag gcggcgatcg accggccctc gagctgcccg
     1141 gcgagcagcc ggagttgatc acccgggtgg ccgcagcgaa tccccgtacg gtcgtggtcg
     1201 tcaatgccgg ttcggccgtc gacctggcct gggaggacga cgtccccgcc gtgctgtacg
     1261 gatggttcgg cggtcaggag gtcggcaacg ccgtggccga cgtcctgctc ggacgatccg
     1321 aacccggtgg ccgactgccc ttctcgatcc cggccgatcc cggcgacatg ctcgacatcg
     1381 gctaccgggg cgacggcggc cctggatggg gaggcaccgg cggcacgatg agctaccgcg
     1441 agggcgacgc gatcggccat cggcaccatg cccgctccgg gcgtcgggca cgccatgggt
     1501 tcgggcacgg cgagtcgtac accaccttct cccacgacga tttccacgtc agggaggagg
     1561 acggtcaact gctggcgcga tgcacggtgc gcaacgtggg cgaccgggaa gggagcaccg
     1621 tcccgcaggt ctacgtgacg gcgccgggcc cgcaccggac ccggcgcctg gtcgggttca
     1681 ccaaggccac cctggccgcc ggggaggaga ccggggtgac gatggcgctc gatccccgca
     1741 cggtcgaatc ctggtcggtc gaggagtcgg actgggcacc ggtcccgggc acccacgtgc
     1801 tcgagctgac cctgtccgca gctgcgccgg cgctggggcg gatcgactac acggtcaggg
     1861 cccgcgactg atcgaggacg tacggcgcgt gccacagcgg ggtgttgctg agtggcagca
     1921 ccgacgcatc caactgcact ccgatggtct cggcaaggta gtcccgccgc tgctggatgc
     1981 ggtcgtacgc ctcggggtac gtctcggcca ggctggcccg cagctcctcg tcggcgagtg
     2041 cgaccccgtc ctcgatgttg gcaccgaccg tcggtccgtc gtactcgggg atgatgtcgc
     2101 cctggagcag cagcccgctg cggaggggcg tcgggttccc gggtgagaac ggtgactcca
     2161 gccactcctc gtagcccagc gtgtggccgg ggttcagcga gaaccggaac aaggagtcgt
     2221 cccggaccgt gtccgcagcg gcatggacct cgccaccggt ggcgccgatc cgtactgccc
     2281 ggtaccaggc caccacggtg tcgaagtagt tgagggtcag tgcctcgaag aactcggccg
     2341 tcgccggctc gaggtccccg ggcccgtggg cgacgatgcc ggagcggcag gtcaggctgc
     2401 ccttgagccc gaacgcgatc tggaaggcgt cgcccagctc cacgcgctgg tcgatcggtg
     2461 agacgacccc gagcctggtc cgttcgccga agttgaccat ggggtgcacc gagcgctgga
     2521 cgcctccgtc ggacagcatg tccgccagcc ggtccgcacg ctcgccgaca gtgatcgccg
     2581 cagcagcacg gtgcacggcg agactgctga tggcgctcgc atactcggcc tcggcgacct
     2641 cgtgtgcggt gttgatgctg cgcagcccaa cttctggatg cacgaacagc tgttgcgcgt
     2701 tccgcacgtg atcagcaccg acgacgttcc ggacggcgtc ggccaggtag ctcggcaccg
     2761 cgagcgaacg ctccggatca ctcacgaagc ccgcgctgaa gctcttgcct ccgacgacac
     2821 ccacctggca gccactgtcc aggccc
//
LOCUS       DATGUK010000021         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_141047,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000021 DATGUK010000000
VERSION     DATGUK010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141047"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1235
                     /locus_tag="VK103_00300"
     CDS             258..1235
                     /locus_tag="VK103_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81947.1"
                     /translation="MDTTATPLPLIPLTDRHRHHPKYLEIERLIREHGFVVLAEHPDL
                     RHQLQYLQRRRLLVPMCPGHLVRAGTEGDPDLRIRVVSAWHPDGVLMGAAAARATFAP
                     DIDVPVVVMASTRKRRSPDGVRLVRWTVPDEHIGEHHGLRFTRPALTALDLARDDDGA
                     TIDEVLRRGLATLAHLHRALRATPYRLGNPVRRFMVDDSRDIPWSPAERLTHRILRAA
                     GITGWSTNLRVSLPQGRHGFIDVAFDDLGIAIEVDGYGHHGHTENRADFEQDRWKRSS
                     LVADGWVVIQLTYRQLTEHLDWVLDVVRRTLRLRGKRPRLSRKACPAAA"
     gene            1245..2258
                     /locus_tag="VK103_00305"
     CDS             1245..2258
                     /locus_tag="VK103_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HLQ81948.1"
                     /translation="MTSVDWSLAETTARRLARPGPEVTHAEAATAVESLRAAAGRAEQ
                     LVTDVTGLEPYGEPAPVHVVDRPTWVSANVASFADLLAPLSERYSTGRVTAGATGLEV
                     GVALAYLSGRVLGQYDPYSEPAGRLLLVAPNIVQAERDLGVRPADFRLWVAVHEQTHV
                     VQFTHALAGRPWLRDHLRARIALLLEQTDLGGELVSTAVRGLTARARGSAGDVSLMDL
                     VTTDEQREMIGEVTAVMSLLEGHADLVMDEVGPEVIPSVALIRSRFEQRRSRGGMDRL
                     LRALLGLDAKLRQYRDGVAFCRAVVERTGMVGLNRVFTAPETLPRPDEIAAPDRWIAR
                     VHP"
     gene            2266..>2476
                     /locus_tag="VK103_00310"
     CDS             2266..>2476
                     /locus_tag="VK103_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HLQ81949.1"
                     /translation="MAKRALGPATLAIVQAVRARCDPERPWLVACSGGRDSLALAAAA
                     GLAGPDRVTALVVDHGLQAGSADVAE"
BASE COUNT          333 a          901 c          879 g          363 t
ORIGIN      
        1 cacccaatcc aggcccacct gcgcgcctgc accggatccg cgcctgcacc ggatccgcgc
       61 ctgcaccccg cgccccatcc ggcccggatt ccgcggaccg accggtcgac gctccagagg
      121 gtgcccggac cgcccccggc gacgggtcga ccggtcggtc cgcggacttg tccccagcgg
      181 ccccagtcca ccgagccctg tccacagatc cgttgcgcgc ccggccgatc cgggcggtcg
      241 cgacgcatct gtgtgacatg gacaccactg ctacgcccct tccgctgatt ccactgaccg
      301 accggcaccg ccaccacccc aagtacctcg agatcgagcg gttgatccgc gaacacggtt
      361 tcgtcgtgct cgccgagcac cccgacctgc gccaccaact ccagtacctg caacgacgcc
      421 gcctgctggt gccgatgtgc ccgggtcacc tggtccgggc cggcaccgag ggtgaccccg
      481 acctgcggat ccgggtggtg tccgcctggc accccgatgg cgtgctgatg ggcgcggccg
      541 cggcccgggc cacctttgcc cccgacatcg acgtcccggt ggtggtgatg gcctccaccc
      601 gcaagcgccg gtcgcccgac ggggtgcggc tggtccgctg gaccgtcccc gacgaacaca
      661 tcggtgaaca ccacggcctc cgcttcaccc gccccgcact gaccgcgctc gacctggccc
      721 gcgacgacga cggggccacc atcgacgaag tgctgcggag ggggctggcc accttggccc
      781 atctgcaccg tgcgctgcgt gcgacgccgt accgactggg caatccggtg cgcagattca
      841 tggtcgacga ctcccgcgac atcccttggt cgccggccga acggctcacg caccggatcc
      901 tgcgggcagc cggcatcacc ggctggtcca cgaacctgcg ggtgtcgctg ccccaggggc
      961 gccacgggtt catcgacgtt gccttcgacg atctcggcat cgcgatcgag gtcgacggct
     1021 acggccacca cggccacacc gagaaccggg cggacttcga acaggatcgc tggaagcgct
     1081 ccagcttggt cgccgacggc tgggtcgtga tccaactgac ctatcgccag ctcactgagc
     1141 atctcgactg ggtgctcgac gtggtccgcc gaaccctccg gctacggggc aagcggccgc
     1201 gcctgtcacg gaaggcctgc ccagcagccg catagagtcg tgccatgacc tctgtcgact
     1261 ggtcgctggc cgagacgacc gcccgccgtc tggcgcggcc gggaccggag gtgacccacg
     1321 ccgaggccgc tacggcggtc gagtcgctgc gagctgcggc aggcagggcc gaacaactcg
     1381 tcaccgacgt gaccgggctc gagccgtacg gggagccggc gcccgtgcac gtggtcgacc
     1441 ggcccacctg ggtgtcggcg aacgtcgcca gcttcgccga cctgctggcc ccgttgagcg
     1501 agcgttactc gaccggccgg gtgaccgccg gggccacggg cctggaggtc ggggtggcgc
     1561 tggcctacct gtccggccga gtgctcggcc agtacgaccc gtacagcgaa cccgcgggcc
     1621 ggttgctcct ggtcgcgccc aacatcgtgc aggccgaacg ggacctcggg gtgcggccgg
     1681 cggacttccg cctgtgggtg gcggtccacg aacagaccca cgttgtgcag ttcacacatg
     1741 cgctggcggg ccggccgtgg ctgcgtgacc acttgcgtgc ccggatcgcc ctgctgctcg
     1801 agcagacgga tctgggcggg gagttggtct ccacggcggt acgggggctg acagcccggg
     1861 cgcggggcag cgccggcgac gtctcgctga tggacctggt caccactgac gagcagcgcg
     1921 agatgatcgg cgaggtgacc gcggtgatgt cgttgctgga gggccacgcc gacctggtga
     1981 tggacgaggt cgggccggag gtgattccga gcgtggcgct gatccggtcc cgtttcgagc
     2041 agcgccggtc ccgcggcggg atggaccggt tgctgcgggc cctgctcggg ctggatgcga
     2101 aactgcggca gtaccgcgac ggggtggcct tctgtcgcgc tgtggtcgag cggacgggga
     2161 tggtggggct gaacagggtg ttcaccgcgc cggagacgct tccgcgtccc gatgagatcg
     2221 ccgcgcccga tcgctggatc gcgcgcgtcc acccgtgatc ccgcgatggc gaagcgtgcg
     2281 ctcggcccgg ccaccctggc gatcgtccag gcggtccgcg cgaggtgtga cccggagcgg
     2341 ccctggctgg tggcctgctc gggtggtcgt gattcgctcg ccctcgcggc agcggccggt
     2401 ctggccggcc cggaccgggt gacggcgctg gtcgtcgacc acgggttgca ggcgggctcg
     2461 gccgacgtgg ccgagc
//
LOCUS       DATGUK010000022         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_954699,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000022 DATGUK010000000
VERSION     DATGUK010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954699"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VK103_00315"
     CDS             complement(<1..530)
                     /locus_tag="VK103_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HLQ81950.1"
                     /translation="MSSSADSPRPADGSTQHTPAQRRSTGGRRGRGDGLRSSFVVVAN
                     RLPVDRVVLDDGSTDWRTSPGGLVSAIAPVMRKNRGAWVGWHGSADEELEPFVDDDLQ
                     LVPVPLSATEVQEYYEGFSNGTLWPLYHDVVEFPEYHREWWDAYVKINKRFASRAADV
                     TQHNGTVWVQDYQLQLV"
     gene            646..1509
                     /gene="otsB"
                     /locus_tag="VK103_00320"
     CDS             646..1509
                     /gene="otsB"
                     /locus_tag="VK103_00320"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HLQ81951.1"
                     /translation="MTAGPAGSSLTDRLLDTLVAALADDPQRAVLATDFDGVLAPIVD
                     HPEHARPHPDALVALARLGHVIGQLVVITGRPARTAVQLGGLDIEPGLRRLVVLGQYG
                     VERWDGATDTYAIPPVPEALGEVRRAVPDLLAEQGWADARIEDKGRALAIHTRTLAEP
                     DRALAELTEPVRRLAEEHGLHLEPGRRVLEVRQSGMDKGVALRSIVAETGARTVIYAG
                     DDLGDVPAFEAVRDLAAEGLTTCAVWSASAERASLPHLTDVAVDGPAGVAAWLTMVAD
                     RVERRLTGNGG"
     gene            1561..1637
                     /locus_tag="VK103_00325"
     tRNA            1561..1637
                     /locus_tag="VK103_00325"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1595..1597,aa:Ile,seq:cat)
     gene            1774..2466
                     /locus_tag="VK103_00330"
     CDS             1774..2466
                     /locus_tag="VK103_00330"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HLQ81952.1"
                     /translation="MAAPSPPDAAIRSVRLGAVLWLLSGVAVVGQLVAASAWQAPPYS
                     WTDHAISDLGVTACGRYDDLGGAVRTICSPWHPVFNAAMVLAGLLIAVGGWLLRSAWA
                     TRLTRIALVLMVPAGLAVAAVGAMPWDLLPDLHDLAATVQWLVQVVAMALCVSLVRDD
                     RPGSRLLAVGTLGAVLVSVVGFGLFLAGPGAQSVIGWGLAERLAFDVLTLWTVAVGAH
                     VLVRQPHRVPAG"
     gene            complement(2485..>2723)
                     /locus_tag="VK103_00335"
     CDS             complement(2485..>2723)
                     /locus_tag="VK103_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015621456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLQ81953.1"
                     /translation="AIVLALSLFGLRTAHHDQAARLSGMAQSIGYLIAAVGPIAIGGL
                     HDVSGSWTTALAPMLAVMAVQLVMGLLAGRDRVL"
BASE COUNT          380 a          970 c          964 g          409 t
ORIGIN      
        1 accagctgca gctggtagtc ctgcacccac acggtcccgt tgtgctgggt gacatcagcc
       61 gcacggctgg cgaaccgctt gttgatcttg acgtaggcgt cccaccactc gcggtggtac
      121 tcggggaact ccaccacgtc gtggtacagc ggccagagcg tgccgttgga gaacccctcg
      181 tagtactcct gcacctcggt cgcactcagc gggaccggca ccagctgcag gtcgtcgtcg
      241 acgaacggct ccagctcctc gtccgccgag ccgtgccagc cgacccaggc accacggttc
      301 ttgcgcatca ccggggcgat ggcgctcacc agtccgccgg gggaggtgcg ccaatcggtg
      361 ctgccgtcat cgagcaccac ccggtcgacg ggcaacctgt tcgccaccac gacgaacgac
      421 gacctcagcc cgtcaccgcg tccgcgtcgg ccacctgtgg aacgccgttg ggccggggtg
      481 tgctgcgtgg agccatccgc gggccgcggc gagtcagcgc tggaactcat ggaaccgatg
      541 cctgcctcct cttcccccgt caaccttagc cggagatctc cgtgccgtga cggtcacccc
      601 atgtgggtgc ggtggaagcc cgccggatct gaaagggttg ggcccatgac agcaggtccc
      661 gccggcagca gcctcaccga ccgtctcctc gacaccctcg tcgcggcgct ggccgacgat
      721 ccgcagcgag cggtgctggc gaccgacttc gacggcgtgc tggcacccat cgtggaccat
      781 cccgagcacg ccagaccaca cccggacgcg ttggtggcgc tggcccggtt gggccacgtc
      841 atcggccaac tcgtggtgat caccggccga ccggcacgta ccgccgtaca gctgggaggc
      901 ctggacatcg agccgggact gcggcggctc gtcgtgctgg gacagtacgg cgtcgagcgc
      961 tgggacggtg ccaccgacac ctatgcgatc ccgccggtgc cggaggcgct gggggaggta
     1021 cggagagccg tcccggacct gctggccgag cagggatggg ccgatgcgcg catcgaggac
     1081 aagggacgtg ccctggccat ccacacccgc accctggccg agcccgaccg ggcgttggcc
     1141 gagctcaccg agccggtccg gcgactggcc gaggagcatg gcctccacct ggaacccggt
     1201 cgtcgggtcc tcgaggtgcg acagtccggg atggacaagg gcgtcgcact gcgcagcatc
     1261 gtcgccgaga ctggtgcgcg gaccgtcatc tacgccggcg acgacctggg tgacgtgccc
     1321 gcgttcgaag ccgtccgtga tctcgcggcg gagggcctga cgacctgcgc ggtgtggtcg
     1381 gcctcggcgg aacgggcgtc gttgccccac ctcaccgatg tcgccgtcga cgggccagcg
     1441 ggggttgccg cctggctgac catggtcgct gaccgcgtcg aacggcggct gactgggaac
     1501 ggtggctgac tgggcacgga tagggtgacc ctgcgtccgg atccccacga tgcggacgac
     1561 ggggcggtag ctcagccggt cagagcaggc gactcataat cgtccggtcg cgggttcaag
     1621 ccccgcccgc cccaccagcg caggccccac cggtgcagcc tcctccgtca gggcctctcg
     1681 accttgctcg agcggcggtg acttcgcccg agggacggcg gggcggcaca atggacgatc
     1741 gtccgtcaac gtgccaccgg gaaggagcgg cccatggctg cgcccagccc tccggacgcg
     1801 gcgatccgat cggtccgact cggggcggtg ctgtggctgc tgagcggtgt cgccgtcgtc
     1861 gggcaactgg tcgccgcctc ggcgtggcag gcaccgccgt actcctggac cgaccatgcg
     1921 atcagcgacc tcggcgtcac ggcgtgcggc cggtacgacg acctcggtgg ggcggttcgc
     1981 accatctgct caccgtggca tccggtgttc aacgccgcga tggtgctggc cggactgctg
     2041 atcgccgtcg ggggctggct gttgcggtcg gcctgggcga cccggctcac ccggatcgcc
     2101 ctggtgctga tggtgcccgc cggcctggcg gtcgcggccg tcggcgcgat gccgtgggac
     2161 ctgctgcccg acctgcacga cctggctgcc accgtgcagt ggctggtgca ggtggtggcg
     2221 atggcgttgt gcgtcagcct ggtgcgcgac gaccgaccgg gctcacgcct gctcgcagtc
     2281 ggcacgctcg gcgcggtgct ggtgtcggtg gtcggcttcg ggctgttcct cgccggaccc
     2341 ggagcgcagt cggtgatcgg gtggggactg gccgaacggc tcgccttcga cgtgctgacc
     2401 ctgtggacgg tcgccgtcgg tgcccacgtc ctggtccgtc aaccgcaccg ggtcccggcc
     2461 ggatgaggcg cccgacccga gcgctcacag cacccggtcg cggccggcca gcaaccccat
     2521 caccagctgt accgccatca ccgccagcat cggtgccagt gcggtcgtcc agctgccgga
     2581 cacatcgtgc agcccaccga tcgcgatggg accgacagcc gcgatcaggt agccgatcga
     2641 ctgcgccatc ccggacagcc gggccgcctg gtcgtggtgc gcggtccgca gcccgaacag
     2701 cgacagggcc agcacgatgg ccg
//
LOCUS       DATGUK010000023         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_837768,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000023 DATGUK010000000
VERSION     DATGUK010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837768"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VK103_00340"
     CDS             <1..368
                     /locus_tag="VK103_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81954.1"
                     /translation="RRPQRARSAIELAWDDVMRAGGMRQLLVTGLLSGVAGTWVVVFT
                     TALFHEAPVPATVVLLALGVLSAVAAFGVPLPFWVGRLHSYPLRRLWPTGLDGARRGS
                     TGADGARRGSTGVDGVASC"
     gene            362..724
                     /locus_tag="VK103_00345"
     CDS             362..724
                     /locus_tag="VK103_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLQ81955.1"
                     /translation="MLIIDPASQVPPYEQLRRQVVDQISSGELAVGSRLPAVRRLAGD
                     LGLAPNTVARAYRELESQGYVRTAGRNGTLVADQAADGKVARRALELTVDYVRSMAGL
                     GLGGDDIVRYVHRGLSAR"
     gene            772..1194
                     /locus_tag="VK103_00350"
     CDS             772..1194
                     /locus_tag="VK103_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLQ81956.1"
                     /translation="MTRWVVSIKGVLLEDDRVLLGLNDRDEWELPGGQLDPGEQPADA
                     LVREFAEETGLAVRVGQLLDAYRFEVVPGRSVVIIAHLCERTDPAQRPVGSHEHRRLV
                     HHRVADLDRIALPDGYRRVIRAASRGMTAGGASPGVPR"
     gene            complement(1201..2145)
                     /locus_tag="VK103_00355"
     CDS             complement(1201..2145)
                     /locus_tag="VK103_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLQ81957.1"
                     /translation="MSGHHHGHGIAATATPGHRTRLAIALGITSAVLLAQLVGTVLTG
                     SLALLVDLVHMITDAGGLVLALVASVLITRPASKRRTWGFRRAEVIAAGLQAGILLAV
                     AVFAAVEAVQRLLAPPQVDGSLLVVFGVVGLVGNVISLAVLSSARDDNLNLRAAFLEV
                     TADALGSVAVIIGAIVVATTGWQRADTVASLVICALIVPRAVALLREAGSILLESTPP
                     GLDLDEVRDHLLALDHVREVHDLHASRVASGLPVLSAHLVVDPECFHDGHTAELLLTV
                     QGCVARHFEVSVEHSTIQFEPVGHAEREHAVCHTLTSS"
     gene            complement(2142..2504)
                     /locus_tag="VK103_00360"
     CDS             complement(2142..2504)
                     /locus_tag="VK103_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010552085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLQ81958.1"
                     /translation="MAMIDERLTAVFHALSDPGRLTILQHLKTGEHRVRDLTDHLHLA
                     QSTVSAHLACLRDCGLVGSRPDGRASLWSLTAEPETDRILEAGAALVGAMAPNDPHHT
                     DAAHRVAILAAPRSEART"
BASE COUNT          367 a          872 c         1008 g          374 t
ORIGIN      
        1 cgcgtcgtcc gcagcgtgcc cggtccgcga tcgagctggc gtgggacgac gtgatgcggg
       61 ccggcgggat gcggcaactg ctggtcaccg gactgctgtc cggggtggcc ggcacctggg
      121 tcgtggtgtt caccaccgcg ctgttccacg aggcgcccgt gccggccacg gtggtgctgc
      181 tcgccctcgg ggtgctgtcg gccgtggcgg cgttcggggt gccgctgccc ttctgggtcg
      241 ggcggctgca cagttacccg ctgcggcgac tctggccgac ggggctcgac ggggctcgac
      301 ggggctcgac gggggccgac ggggctcgac ggggctcgac gggggtcgac ggggtcgcgt
      361 catgttgatc atcgacccgg cgagccaggt cccgccgtac gagcaactcc gtcgccaggt
      421 cgtcgaccag atctcctccg gagaactggc ggtgggcagt cggttgccgg ccgtgcgccg
      481 gctggccggc gacctcgggc tggccccgaa cacggtggcc cgcgcctacc gtgagctgga
      541 gtcgcagggc tacgtgcgca cggccggccg caacggcacg ctggtcgccg accaagcggc
      601 tgacggcaag gtcgcgcgac gcgccctgga gctgaccgtg gactacgtcc ggtcgatggc
      661 ggggctcggg ctggggggcg acgacatcgt gcgttacgtt catcgtgggc tcagcgcacg
      721 atgagggcgg gatgacggag cagaagagga gcagaaaagg aaggtcgccg catgacgagg
      781 tgggtggtca gcatcaaggg cgtgctgctc gaggacgacc gggtgttgct cgggctcaac
      841 gaccgcgacg agtgggagct ccccggtggc cagctcgacc cgggggagca gcccgccgac
      901 gccctggtcc gggagttcgc cgaggagacc gggctcgcgg tccgggtcgg ccagctgctc
      961 gacgcctacc ggttcgaggt cgtcccgggc cgttcggtgg tgatcatcgc ccacctgtgt
     1021 gaacggaccg acccggcgca acgaccggtc gggtcccacg agcaccgacg gttggtgcac
     1081 catcgggtcg ccgacctcga tcgcatcgcg ctgccggacg ggtaccgccg ggtgatccgg
     1141 gcagcctccc ggggtatgac cgctgggggc gcgtcacctg gcgtcccccg gtgagtgctg
     1201 tcagctgctg gtgagggtgt ggcacactgc gtgctcacgc tcggcgtgcc cgacgggctc
     1261 gaactggatc gtggagtgct cgaccgacac ctcgaagtgc cgggcgacgc agccctgcac
     1321 ggtgagcagc agctcagcgg tgtggccgtc gtggaagcac tccggatcga ccaccaggtg
     1381 ggccgacagc acggggagcc cactggccac tcgggaggcg tgcaggtcgt gcacctcccg
     1441 tacatggtcc agggccagca gatggtcgcg cacctcgtcc aggtcgagac cgggcggcgt
     1501 cgactccagc aggatcgagc cggcctcacg cagcagcgcc accgcacgcg gcacgatcag
     1561 cgcacagatc accaggctgg ccacggtgtc ggcgcgttgc cagccggtgg tcgccaccac
     1621 gattgcgccg atgatcaccg cgactgagcc gagtgcatct gcggtgacct ccaggaaggc
     1681 ggcccgcagg ttgaggttgt cgtcccgggc cgaggacagc accgcgagcg agatcacgtt
     1741 gcccaccagg ccgaccacgc cgaagaccac cagcagggag ccgtccacct ggggtggcgc
     1801 gagcaggcgc tggacggctt cgaccgcggc gaacaccgcc accgccaaca ggatgcctgc
     1861 ctgcagcccg gccgcgatca cttcggcgcg gcggaacccc caggtgcggc gcttcgacgc
     1921 cggtcgggtg atcagcaccg aggcgaccag ggcgagcacc aggccgccgg catcggtgat
     1981 catgtgcacc aggtcgacca gcagcgccag gctgccggtc aggaccgtac cgacgagttg
     2041 ggcgagcagg actgcgctgg tgatccccag cgcgatcgcg aggcgggtgc gatgcccagg
     2101 agtggcggtc gcggcgatgc cgtggccgtg gtggtgtccg ctcacgtccg ggcctccgac
     2161 cggggcgccg ccaggattgc cacccggtgg gctgcgtcgg tgtggtgcgg gtcgttcggg
     2221 gccatcgcgc ccaccagggc cgcgcccgcc tcgaggatcc ggtcggtctc gggctccgcg
     2281 gtcagcgacc acagggaggc ccgtccgtcc ggacgtgagc ccaccaggcc acagtcacgc
     2341 aggcacgcca ggtgagcgct caccgtgctc tgggccaggt gcaggtggtc ggtgaggtca
     2401 cggacccggt gctcaccggt cttcaggtgc tgcaggatcg tcaggcggcc ggggtcggac
     2461 agcgcatgga acaccgctgt cagccgctca tcaatcatcg ccatgtgtcg atgatagcgc
     2521 cagttgccgt ttgctgggtc ggtggtcgac aggtggtcgg cagccggggg tctcggcccg
     2581 ggcgtgggcg tcgcaaggtg ttgggcgctg cgcggggcgt c
//
LOCUS       DATGUK010000024         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_142920,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000024 DATGUK010000000
VERSION     DATGUK010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142920"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VK103_00365"
     CDS             complement(<1..729)
                     /locus_tag="VK103_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLQ81959.1"
                     /translation="MTNTFALPRRSFLAIPLGVAAVSAAGCGFSGSQDNGGTGPDGTD
                     PTTTTLRQANRPVNHLNPAFGGGGSPQSTWLMSMIWEGLINRKTDKPSEYIPAVAEEW
                     EVSDDGLTYTFHLRDGLTWSNGDPLTAEDFVWSYSYYYSPALAEQDNENEPSHKGNVE
                     STKIAGMADYYSGKVSDFSTVGVKAIDDTTLEITLFEPDYSMLNGLDGFYPLHRTSVE
                     ADQKAFWMPENLVANGPYVMTDYTQ"
     gene            complement(872..2809)
                     /locus_tag="VK103_00370"
     CDS             complement(872..2809)
                     /locus_tag="VK103_00370"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HLQ81960.1"
                     /translation="MQGFGPDLDNYADPELDLERYVHGRTATWLDHWRSRDDPTPEGA
                     RRHVERVRDAVVTGLGGLPERSGPPPVTVTGRHVDAAARQVVENISFESLPGVRVTAN
                     LYRPDGVSPQQPAPAVLFACGHAYAAKAAERYHLVCRSLAAAGMTVLAVDPHGQGERV
                     LDPDHDPDSGRGWGVDEHLRTGTAAWWAGRSLMAWMVADLMAGVDLLAALPQVDPDRI
                     GITGNSGGGTQASIMLAVEPRLAAAALGTYVTGRREYQRMGQTQDPEQHLMGGTVAGV
                     DHADLLISFAPRPLRVLAVAWDFFPLEGAREAVDRAGAAYAALGAADRLSLTVDDSVH
                     TYSVGMEASAVEFFCETFGLPVPSDPVVGPGGPTPDQLRVTRSGQVLADDPDAVMITD
                     LVARYLDTLRDSGELAPWLGERVHRHRRRPPDDGVRWIGTAEEPRLFWNSERDLWGAA
                     VLLDPEADPGGAAADLTPLTVVLLPDGTRTRDADRLLPAAGTGPRFVLDLRGQGALTS
                     RQRGRHPRASLRGYDFKLLCDLIWLDDSLAAGRVFDLIRAVDVLIADRELRRRWPGIG
                     PRSPVRLVTVGGLARWHAELAATIDHRISAVDHDGSALDLDRAIRRADWDEEVGDWQA
                     LVPGIAQRLDLGRAGVQDGGS"
     gene            2890..>3697
                     /locus_tag="VK103_00375"
     CDS             2890..>3697
                     /locus_tag="VK103_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLQ81961.1"
                     /translation="MTRRYALCGLSNRGIASFALPMLGLDRDGAPSEEDHSAVAELVA
                     VVEPDAVRAAAFDSWLTDHGRPPLPRHDSVDALLADPDAPELDALVVASPDHTHIDHL
                     RAGLAHGLDVVTEKPMVTTTEDARAVLAAEAASTGRVLVTHNFRYPPRHLQLKELLRA
                     GRIGRVVQVLLEYHVDTSHGASYFVRWNRATAASGGLTLHKSTHHLDLVGWLLDDEPV
                     RVAAAGGRWFYGPDSPHRPRAADGTALTGTELRAADPYWRTMVDRGAVAEP"
BASE COUNT          531 a         1378 c         1245 g          543 t
ORIGIN      
        1 ctgggtgtag tcggtcatca cgtacggccc gttggccacc aggttctccg gcatccagaa
       61 ggccttctgg tccgcctcca ccgacgtgcg gtgcagcggg tagaacccgt ccagcccgtt
      121 caacatgctg tagtcgggtt cgaacagggt gatctccagg gtcgtgtcgt cgatcgcctt
      181 caccccgacg gtggagaagt ccgacacctt gcccgagtag tagtcggcca tcccggcgat
      241 cttggtggac tcgacgttgc ccttgtggct cggctcgttc tcgttgtcct gctcggcgag
      301 cgcgggcgag tagtagtacg agtacgacca cacgaagtcc tcagcggtga gtgggtcgcc
      361 gttggaccag gtcaggccgt cacgcaggtg gaaggtgtag gtgaggccgt cgtcggacac
      421 ctcccattct tcagcgaccg cagggatgta ctccgagggc ttgtcagtct tgcggttgat
      481 caagccctcc cagatcatcg acatcaacca ggtcgactgc ggtgagccgc cacccccgaa
      541 ggcggggttg aggtggttca ccggtcggtt cgcctgccgc agcgtggtcg tcgtcgggtc
      601 cgtgccgtca ggtccggtcc ccccgttgtc ctggctgccg ctgaacccgc agccggcggc
      661 gctcaccgcg gccaccccca aggggatcgc caggaacgat cgcctcggca gcgcaaacgt
      721 gttggtcatc ggtctccccc tccggcgaac cgcgtgcccc gcggatccgg tgcggacacc
      781 accccgtcga ggtgtccgtg ctgcgaacgt acaagcatgg cagcaacaat gcaacagttg
      841 tgccactgcc cgaaacccgg gtgggtcgtc atcatgaccc gccgtcctgc accccggcac
      901 ggccgaggtc gagacgttgc gcgatgccgg ggaccagggc ctgccagtcc cccacctcct
      961 cgtcccagtc cgcccggcgg atcgcccggt ccaggtccaa cgccgaccca tcgtggtcga
     1021 ccgcactgat ccggtggtcg atcgtggcgg ccagctccgc gtgccagcgg gccaacccgc
     1081 cgaccgtgac cagccggacc gggctccggg ggccgatccc cggccagcgg cggcgcagct
     1141 cccgatcggc gatgagcacg tcgacggctc ggatcaggtc gaagacccga ccagcagcca
     1201 agctgtcgtc cagccagatg aggtcgcaca acagcttgaa gtcgtagccc cgcagggacg
     1261 cccgtggatg ccttcctcgt tgccgcgagg tgagcgcccc ctgtccacgc aggtccagca
     1321 cgaaccgcgg gcccgtcccg gcggcgggca gcagccggtc cgcgtcccgg gtgcgggtgc
     1381 cgtcgggcag cagcaccacg gtcagcgggg tcaggtcggc ggcggcgccg ccggggtccg
     1441 cctccgggtc caacaacacc gcggcgcccc acaggtcgcg ttcgctgttc cagaacagcc
     1501 gtggctcctc ggcggtgccg atccaccgga caccgtcgtc gggtggtcgg cggcggtgcc
     1561 ggtgcacccg ctcacccaac cacggcgcca gttccccgct gtcgcgcagg gtgtcgaggt
     1621 agcgcgccac caggtcggtg atcatcaccg cgtccgggtc gtcggccagc acctggccgg
     1681 aacgggtgac ccgcagttgg tccggggtcg gcccgccggg cccgaccacc ggatcggagg
     1741 gtacgggcag accgaacgtc tcgcagaaga actcgacggc ggaggcctcc atgccgaccg
     1801 agtacgtgtg cacgctgtcg tcgacggtca gcgacagccg gtcggcggcc cccagggcgg
     1861 cataggccgc acccgcacgg tccaccgcct cccgggcacc ctccaacggg aagaagtccc
     1921 acgccaccgc cagcacccgc agcggtctgg gggcaaaact gatcagcaga tcggcgtggt
     1981 cgacaccggc caccgtgccg cccatcaggt gttgctcggg gtcctgtgtc tgccccatcc
     2041 gctggtactc gcgacggccg gtgacgtagg tccccagggc cgccgccgcc aaccgtggct
     2101 ccaccgccaa catgatcgag gcctgcgtcc caccgccgct gttgccggtg atgccgatcc
     2161 ggtccgggtc gacctgcggc agtgccgcga gcaggtccac ccccgccatc aggtcggcga
     2221 ccatccacgc catcagcgag cgtccggccc accaggcggc ggtcccggtg cgcaggtgct
     2281 cgtccacacc ccagccccgg ccggagtccg gatcgtggtc ggggtccagc acccgctcgc
     2341 cctgtccgtg cggatcgacc gccagcacgg tcatcccggc cgccgccagc gagcggcaga
     2401 ccaggtggta gcgctcggcc gccttcgccg cgtacgcgtg tccacaggcg aacaggaccg
     2461 ccggcgccgg ctgctgcggc gacaccccgt caggtcggta gaggttcgcg gtcacccgca
     2521 ccccgggcag cgactcgaag ctgatgttct ccaccacctg gcgggccgct gcgtcgacgt
     2581 gtcgcccggt cacggtcacc ggcggcggcc cggatcgctc cggcaggccc ccgagaccgg
     2641 tgacgacggc atcccggacc cgttcgacgt gacgccgggc cccctcgggg gtggggtcgt
     2701 cccgggatcg ccagtgatcc agccaggtcg cagtgcgccc gtgcacgtag cgctcgaggt
     2761 cgagctcggg gtcggcgtag ttgtcgagat caggcccgaa tccctgcatg ttgtgatcgt
     2821 tgcgcgcccg accgcccctg tcaatgggat tgcagcaatc accacaacac gttgcggtac
     2881 ggtgatgtca tgactcgccg ctacgccctg tgcgggctgt ccaaccgcgg catcgccagc
     2941 ttcgccctgc cgatgctcgg cctcgaccgc gacggcgccc cgtccgagga ggaccactcc
     3001 gcggtcgccg agctggtcgc cgtggtggag cccgacgccg tacgggccgc cgcgttcgac
     3061 tcctggctca ccgaccacgg ccgaccgccg ctgccgcgcc acgactcggt cgacgccctg
     3121 ctggccgatc ccgacgctcc cgaactcgat gcgctggtgg tggcctcccc cgaccacacc
     3181 cacatcgacc acctgcgggc gggcctggcg cacgggctgg acgtggtcac cgagaagccg
     3241 atggtgacca ccaccgagga tgcccgtgcc gtgttggcgg ccgaggcggc cagcaccggg
     3301 cgggtgctgg tcacccacaa cttccgctat cccccgcgcc acctgcagct caaggaactg
     3361 ctccgtgcag gacggatcgg gcgcgtggtg caggtgctgc tggagtacca cgtcgacacc
     3421 tcccacgggg ccagctactt cgtccggtgg aaccgggcca ccgccgcctc cggcgggctc
     3481 accctgcaca agtcgaccca ccacctcgac ctggtcggct ggctgctcga cgacgagccg
     3541 gtccgggtcg ctgcggccgg tggtcggtgg ttctacggtc ccgacagccc gcaccggcca
     3601 cgtgccgccg acggcacagc gctcaccggg accgagctgc gggcggccga cccgtactgg
     3661 cggaccatgg tggatcgcgg tgccgtggcc gagcccc
//
LOCUS       DATGUK010000025         3783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_29574,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000025 DATGUK010000000
VERSION     DATGUK010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29574"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VK103_00380"
     CDS             complement(<1..149)
                     /locus_tag="VK103_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLQ81962.1"
                     /translation="MTPAVSVVIPTHDRDVLLPRAIESALLQGSAVCEVVVVDDLDRA
                     GTRTVV"
     gene            complement(146..1036)
                     /locus_tag="VK103_00385"
     CDS             complement(146..1036)
                     /locus_tag="VK103_00385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLQ81963.1"
                     /translation="MLCDHGAMTDPVRPPAATDDGTQWPSVTVVTVSFRDPEGLRRTR
                     SSLLAQHYPGRLQHVVVDGGTGAEVSDWLANQTGDLSWVSEPDGGIYHAMNKGIAMAD
                     GDLLWFMNSADTFHADDSVRTAMAALRPGTDPREQWGFGRAEWHRPGSREADSVHGPR
                     RYLRVLHALGRQTIPHQAVFLGASLVARLGRYEPEVGITADQALMMRCAREVAPVVLP
                     DLLCDYDMTGVSSQQTRAEHWAAMRRARRLSGVRVTGSRRLDDLLSRALQGVERFRER
                     RPRQVAQPVVPPPMAQGDRP"
     gene            1183..2538
                     /locus_tag="VK103_00390"
     CDS             1183..2538
                     /locus_tag="VK103_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81964.1"
                     /translation="MTVRHALRRMLSGGGVRIIGIPVTAVLGLLNTGLVVAHTGSVTY
                     GIVNTIATLGVLLPFADLGMGVSVTTAVGIATTDPGHRTTATATVRAVLTRLCLVALI
                     GLALVWTVTLTGGWSVVLGRDFSGTESLLVGLAVSLFFLALPCGVGSRALVGLDRNHV
                     VVAVNITATGWALVCTLLLAAAGAHGIPYVLSGFTGTLVSNAVLLVIALVRLRRRGMG
                     LGRRGMATTDGPDRPTRLLAGSGWMLLIMIGLPVGLETGRLVLAHRAVPVELSLFALG
                     AQLYALTWSVLSTSGQALWPIFARQRGALAGNLRLWRHLVLLLGAAAVVGGACLVLVG
                     PWLSGVISQGELRVGRGQMVAFAALLLVQALHLPAGMMLTRPEELRWQAGCVAVMAAI
                     SITVSITWAPAFGGVAVTTGAAAGVLLGQFLPDLVMASRLLRRRPGVAQSDAATAVTP
                     A"
     gene            complement(2502..>3783)
                     /locus_tag="VK103_00395"
     CDS             complement(2502..>3783)
                     /locus_tag="VK103_00395"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HLQ81965.1"
                     /translation="GNATHTWPGGSPMSRREASTTRRSQRRTMARRLAGLVTRAAVVA
                     MRLAVRVLPTVRIAVVHGFPDTEENSLRIAAELRRTLPEPWRVVLLCDDPVVAVRAYR
                     TLEPTAPPVTLRRRLGSAGYLTAMIARVWVHTHGLYGSPRGGRGRCHVLVGHGHGPKQ
                     AVPYGVAHRPQHADLAFTNTRVWGSTVIDDLGVPATAPRVVLASPRSDPVPAVTDDPL
                     ELSRTVLWLPTVRRTRAMLRGQWQDGTPVTEQAGLVEELSQLCAAARRRGLRVATKAH
                     ALDADAAAFDRLGVRVVTGEQLLAADVGFQQFVGSVAGLLTDYSSVFVDHLHTALPIA
                     FWTPDVDEFARDRGFNEPDFRTVAPELFLRDRSAVDEFLDAVAAGTPWRSQARTALAT
                     AIGLAPVDPAGGATRAVVRRILAQAGVTAVAASD"
BASE COUNT          498 a         1381 c         1360 g          544 t
ORIGIN      
        1 accaccgtac gggtgccggc gcggtccagg tcatcgacca ccaccacctc gcacaccgcg
       61 gacccctgca gcagtgccga ctcgatcgcg cgcggcagca gcacgtcacg gtcatgcgtc
      121 gggatcacca ccgacaccgc aggggtcacg gtcggtcgcc ctgcgccatc ggcggaggca
      181 cgaccggctg cgccacctgg cggggccgac gctcccgaaa ccgttcgacg ccctgcaggg
      241 cgcggctgag caggtcgtcg agccgccgac tgccggtgac gcgcacgcca ctcagccggc
      301 gggcgcgccg catcgccgcc cagtgctcgg cccgggtctg ctgcgaggac acacccgtca
      361 tgtcgtagtc acacaacagg tccggcagca ccaccggtgc cacctctcgg gcgcagcgca
      421 tcatcaacgc ctggtcggcg gtgatgccga cctccggttc gtaccggccg agccgggcga
      481 ccagcgaggc accgaggaac acggcctggt gcgggatcgt ctgccgacca agggcgtgca
      541 gcacccgcag gtaacgccgc ggaccgtgca cgctgtcggc ctcccggctg ccggggcggt
      601 gccactcggc acgtccgaac ccccactgct cacgcggatc ggtgccgggg cgcagcgccg
      661 ccatcgcggt ccggaccgag tcgtcggcgt ggaaggtgtc ggcggagttc atgaaccaca
      721 gcagatcacc gtcggccatg gcgatgccct tgttcatggc gtggtagatc ccgccgtcgg
      781 gttcgctcac ccagctgagg tcgccggtct ggttcgccaa ccagtcactg acctcggccc
      841 cggtgccgcc gtccaccacg acgtgctgca gtcgtcccgg gtagtgctgg gcgagcaggc
      901 tggaccgggt gcgccgcagt ccctccggat cacggaacga caccgtcacc acggtcaccg
      961 acggccactg cgtgccgtcg tccgtggcgg ccggtggccg taccggatcg gtcatcgcgc
     1021 catgatcaca cagcatcccg gtcccggggt acgactgtcg caccgccgtc ctgcgtggtg
     1081 tcgcggatcg tgccggtggt tcaatccccc ctcaggacgg cccgatgccg tcccgcgacc
     1141 gacagggggc cgatggcgcc gacgagtggg ggcggacagg gcgtgaccgt acggcacgcc
     1201 ctgcgacgca tgctgtccgg tggcggcgtg cgcatcatcg gcatcccggt gacggcggtg
     1261 ctggggctgc tcaacaccgg actggtcgtc gcccacaccg gctcggtgac ctacggcatc
     1321 gtcaacacca tcgccaccct cggtgtactg ctgccgttcg ccgatctcgg gatgggggtc
     1381 tcggtcacca ccgcggtcgg catcgccacc accgatcccg gccaccgcac caccgcgacc
     1441 gcgaccgtac gggccgtgct cacccggctg tgcctggtcg cgctcatcgg gctggcgctg
     1501 gtgtggacgg tgacgctcac cggcggatgg tcggtggtgc tgggccgcga cttcagcggc
     1561 accgagtcgc tgctggtcgg gctcgcggtg tcgctgttct tcctcgcgct gccgtgcggg
     1621 gtgggatcac gcgccctggt cgggctggac cgcaaccacg tcgtggtggc ggtcaacatc
     1681 accgccaccg ggtgggccct ggtgtgcacc ctgctgctcg ccgccgccgg ggcgcacggg
     1741 atcccgtacg tactgtccgg cttcaccggc accttggtga gcaatgccgt actgctggtc
     1801 atcgccctgg tccggctccg tcgccgaggg atggggctgg gtcgccgcgg aatggccacc
     1861 acggacgggc ccgaccggcc gacccggttg ttggccggat ccggctggat gttgctgatc
     1921 atgatcggcc tgcccgtcgg actggagacc ggacggctcg tgctggcgca ccgggccgtg
     1981 ccggtggaac tgtcgctgtt cgccctcggg gcccagctgt acgccctcac ctggtcggtg
     2041 ctcagcacct ccggacaggc gctgtggccg atcttcgccc ggcaacgcgg cgccctcgcc
     2101 ggaaacctgc ggctgtggcg acacctggtg ctgctgctgg gggccgcggc cgtggtcggc
     2161 ggggcctgcc tggtgctcgt cggaccgtgg ctgtcgggcg tgatcagcca gggggagctg
     2221 cgtgtcgggc gcggccagat ggtggcgttc gcggcgctgt tgctggtgca ggcgctgcac
     2281 ctgccggccg ggatgatgct gacccgtccg gaggagctgc gctggcaggc cggctgcgtc
     2341 gccgtgatgg ccgcgatcag catcaccgtc tcgatcacct gggcgccggc attcggcggc
     2401 gtcgcggtca ccacgggcgc ggccgccggg gtgctgctcg gccagttcct gcccgacctg
     2461 gtgatggcgt cacgcctgct gcgccgccga cccggggtgg ctcagtccga tgccgcaacg
     2521 gccgtgaccc cggcctgagc caagatccgg cgcaccaccg cccgggtggc gccaccggcg
     2581 gggtcgaccg gggccagccc gatcgcggtc gccagggcgg tccgggcctg cgaccgccac
     2641 ggggtgccgg cagcgaccgc gtccaggaac tcgtccaccg ccgatcgatc acgcaggaac
     2701 agttccggtg cgacggtacg gaagtccggc tcgttgaagc cgcggtcccg ggcaaactca
     2761 tccacgtccg gtgtccagaa ggcgatcggc aacgcggtgt gcaggtggtc cacgaacacg
     2821 ctggagtagt cggtgagcag accggcgacc gatccgacga actgctggaa tccgacatcg
     2881 gcggcgagca gctgttcacc ggtgaccacc cgcaccccca accggtcgaa ggcggcggca
     2941 tcggcatcca gggcgtgcgc cttggtcgcc acccggagcc cacgtcggcg tgcggccgca
     3001 cacagctggg acagctcctc gaccaggccc gcctgttccg tcaccggggt gccatcctgc
     3061 cactggcccc gcagcatggc gcgggtccgt cggaccgtcg gcagccacag cacggtacgg
     3121 gacagctcca gcgggtcgtc ggtcaccgct gggacggggt cggagcgggg cgaggccagc
     3181 accacccgcg gtgcggtcgc cggcaccccc aggtcgtcga tcaccgtgct gccccacacg
     3241 cgggtgttgg tgaaggccag gtcggcatgc tgggggcggt gggcgacgcc gtacgggacc
     3301 gcctgcttgg ggccgtgccc gtggccgacc agcacgtggc agcgaccccg accaccccgg
     3361 ggtgagccgt acagcccatg ggtgtgcacc cagacccggg cgatcatcgc cgtcaggtag
     3421 ccggccgacc cgagtcggcg ccgcagtgtc accggcggcg cggtcggctc cagggtgcgg
     3481 tacgcccgta ccgcgacgac gggatcgtcg cacaacagca ccacccgcca cggctccggc
     3541 agcgtgcggc gcagctccgc cgcgatccgc agtgagttct cctcggtgtc ggggaagccg
     3601 tgcaccacgg cgatccggac ggtcggcagc acccgtacgg cgagccgcat cgccaccacg
     3661 gcggcgcggg tgaccagacc tgcgagccgg cgcgccatcg tcctgcgctg cgaccgccgg
     3721 gtggtgctgg cctcacgcct cgacatcggt gatccgcccg gccaggtgtg ggtggcgttc
     3781 ccg
//
LOCUS       DATGUK010000026         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_496409,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000026 DATGUK010000000
VERSION     DATGUK010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496409"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VK103_00400"
     CDS             <1..230
                     /locus_tag="VK103_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HLQ81966.1"
                     /translation="DTMPRGMLGMSADSCEQYLHNIANRRCGQLGLEPVFPATTNPFP
                     WMSEVMDLGKEKNFFESRVIEYQTGSGLSWD"
     gene            complement(374..682)
                     /locus_tag="VK103_00405"
     CDS             complement(374..682)
                     /locus_tag="VK103_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLQ81967.1"
                     /translation="MDNFLGWADASIAGLRGSGSYGVAKAAMHGQVWDLARRLAGDGI
                     TVNAVAAGFVPPSSGVAGWTTGPVLQVNGGDLSGTAENHAGVICPPAGDRPVDNFPGR
                     "
     gene            806..1324
                     /locus_tag="VK103_00410"
     CDS             806..1324
                     /locus_tag="VK103_00410"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HLQ81968.1"
                     /translation="MEETQRPEARRLSDVQRRPHGSGDGWVDGPEGRYWGLFGAAGLA
                     VVTPRFGVLLQHRAGWSHFGGTWGLPGGARQEGETSVQAALREAGEEAGVPAEQVRVF
                     GTEVLDLGYWSYTTVLGIADVPFTPVIADAESDDLQWVDLAEVTDRPLHPGLATAWPG
                     LQQRIRAELAAR"
     gene            complement(1338..2294)
                     /locus_tag="VK103_00415"
     CDS             complement(1338..2294)
                     /locus_tag="VK103_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81969.1"
                     /translation="MSWFRRRQRKRDDPELGRPDDHLRPLTVGDAQDLTELVRVAFAA
                     EGVEVVPDGRGALIGDHRTYGLTNLAALVAQQPRRHWSRLARRHARGILAAHEADDEP
                     IDTDSARSRVLLKLRPVNDVGRGVHYAPEILPGILLLVAIDHPDHVRELFSDDAVDEL
                     GGWHEVRDLAVSNLRALPQPAHKVIRADPERGDSIVHLFAADDFFGAARLAILPEVLA
                     HVGIERPAHGVLLAVPNRHLLLVHPLEGPGVIPAVQLMVRMATAEFQGHTGPLSPQVY
                     YRSADGEVQQVTTQDEDEGSVAVRVEGPFAEAFDAMGLTSDD"
     gene            complement(2361..2789)
                     /locus_tag="VK103_00420"
     CDS             complement(2361..2789)
                     /locus_tag="VK103_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81970.1"
                     /translation="MTVKRTILAVAAGLLTVLALSMFATRPTTTFSTGLEGGDVVVSC
                     HPVSVIDDSSRPLDGLGSRRYTYRIEEGRDVLDRLDSGTQTGRDRTSAFALTDRIEAH
                     CDRRRTGRVAWMVLALTPAAVLATLAVTLPAIRAARDYRI"
     gene            2955..3031
                     /locus_tag="VK103_00425"
     tRNA            2955..3031
                     /locus_tag="VK103_00425"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2989..2991,aa:Ile,seq:gat)
BASE COUNT          495 a         1145 c         1195 g          503 t
ORIGIN      
        1 gcgacaccat gccgcgcggg atgctgggga tgagtgcgga cagctgtgag cagtacctgc
       61 acaacatcgc gaaccggcgg tgcggccagc tcgggctgga gccggtgttc ccggccacca
      121 cgaacccgtt cccgtggatg agcgaggtga tggacctggg caaggagaag aacttcttcg
      181 agtcccgggt catcgagtac cagaccggct ccggcctgtc ctgggactga cctgtcccca
      241 actctgcggc tgtggcgtgc cgccgttgtc catggcgcaa cggcgtgccg ctacagccgc
      301 agagttgggg ttggcgtccc agcctgcgac cgggcccggc cggtcgagcg tggcgggtgg
      361 agcgaccaca gcctcagcgg cccggaaagt tgtccacagg cctgtcccca gccggtggac
      421 aaatcacacc ggcgtggttc tcagccgtgc cggacaggtc gccaccgttg acctgcagca
      481 ccggaccggt ggtccagccg gccaccccag aactcggtgg tacgaacccg gctgccaccg
      541 cgttcaccgt gatcccgtca ccggcgaggc gtcgggcgag gtcccacacc tgcccgtgca
      601 tggcggcctt ggcgacgccg tacgatccgc tgccgcgcag tccggcgatc gaggcatccg
      661 cccacccgag gaagttgtcc acaggtgtgt ccccagcccg tggacaaatc acacctgtgt
      721 cgttcgggtc gccgacgggg actgatcgac gactcggcga cggaggggtg ccgcgattgc
      781 tggtcgacac gggacactgg gggcgatgga ggagacacag cgccccgagg cccgccggct
      841 gtccgatgtg cagcgtcgcc cgcacggcag cggtgacggc tgggtggacg gccccgaggg
      901 ccggtactgg ggactgttcg gagcggccgg gctggctgtg gtgacgccac gcttcggcgt
      961 gctgctgcag caccgggccg gctggagcca cttcggcggc acctgggggc tgcccggtgg
     1021 ggcgcgtcaa gagggcgaga cgtccgtgca ggccgccctc cgggaagccg gtgaggaggc
     1081 cggcgtcccc gccgagcagg tccgggtgtt cggcacggag gtcctggacc tgggttactg
     1141 gtcctacacc accgtcctcg ggatcgccga cgtgccgttc accccggtga tcgccgacgc
     1201 cgagagtgat gacctgcagt gggtcgacct ggccgaggtc accgatcgtc cgctgcaccc
     1261 cgggctggcc accgcctggc cgggcctgca gcagcggatc cgcgccgagc tcgccgcccg
     1321 ctgaccggcc ggcacgatca gtcgtcggac gtgaggccca tcgcgtcgaa cgcttcggcg
     1381 aacggaccct ccacccgtac cgccacactg ccctcgtcct cgtcctgggt cgtgacctgc
     1441 tgcacctctc cgtcggcgga gcggtagtac acctgcggtg acagcggccc ggtatggccc
     1501 tggaactcgg cagtggccat ccgcaccatc agctgcaccg ccgggatcac gcccggcccc
     1561 tccaacgggt gcaccagcaa caggtgccgg ttcggcacgg cgagcagcac accgtgcgca
     1621 gggcgttcga tgccgacatg cgcgagcacc tccgggagga tcgcgagccg tgcggccccg
     1681 aagaagtcgt cggccgcgaa caggtggacg atgctgtcgc cgcgttccgg atcagcccgg
     1741 atcaccttgt gtgccggctg tggcagggcg cgcaggttgc tgacggcgag gtcacgcacc
     1801 tcgtgccagc caccgagctc gtcgacggcg tcgtcgctga acagctcacg tacatggtcg
     1861 gggtggtcga tggccaccag cagcagtatg cccggcagga tctccggggc gtagtgcact
     1921 ccccggccga cgtcgttgac cggacgcagc ttgagcagca cccgcgaccg ggccgagtcg
     1981 gtgtcgatcg gctcgtcgtc ggcctcgtgg gcggccagga tgccacgggc atgtcgccgg
     2041 gcgagccggg accagtgccg gcggggctgc tgtgcgacga gtgccgccag gttggtgagg
     2101 ccgtacgtgc ggtggtcacc gatcagggcg ccgcgaccgt ccggcaccac ctccacgccc
     2161 tccgcggcga acgccacccg gaccagttcg gtgaggtcct gtgcgtcacc cacggtgagg
     2221 ggtctcagat ggtcgtcagg tcgacccagt tccggatcgt ctcgcttgcg ttgccgccgg
     2281 cggaaccatg acatggcacc cagcttgtca cgccgcacca gcccgctgcc atgtcgggcg
     2341 accgggaagg gcatccgcgg tcagatgcgg tagtcgcggg ccgcccggat cgccggcagc
     2401 gtgacggcca gggtggcgag caccgcagcg ggcgtcagcg ccagcaccat ccaggcgacg
     2461 cgaccggtcc gtcggcggtc gcagtgggcc tcgatgcggt cggtgagagc gaacgcagac
     2521 gtcctgtccc gcccggtctg cgtcccgctg tcgagtctgt ccagcacgtc cctgccctcc
     2581 tcgatgcggt aggtgtaccg gcgcgaaccg agcccgtcca gcggccgcga actgtcgtcg
     2641 atgaccgaga ccgggtggca cgagacgacc acgtcccctc cctcgagacc ggtggagaag
     2701 gtggtggtcg ggcgggtggc gaacatggac agtgccagta cggtcagcag tccggcggcg
     2761 acggccagga tggtgcgctt cacggtcacc ttctggggat cggggtcggg tggttcgcgt
     2821 gacgctagtg atgccgtccc gtcccgacca cccggtcgga gccgggtggg agcgggtggc
     2881 tcgctccagt ttggggctgt cggacccgat cggctaaagt tgcgcctcgg tacgtcttcg
     2941 acgtcacccg ccccgggcct atagctcaga cggttagagc gctgtcctga taagacagag
     3001 gtcggtggtt caagcccacc taggcccacc acagttcggt atcgcggcat cacccagcac
     3061 acatggcatc gtcccacatc gtgggcggca tccggccatc gcccggttca gccgatccct
     3121 ccgtccgagc gggaccagct ggagggcagg gacaccaagg acgccagggt aggcgtggac
     3181 accaaggacg ccagggtagg cgtggttcgg cccgtcagta gggttcggcc atgaccgagc
     3241 accgccggac cggcgtcgcc cttgccgtct gcgtggtggt gttcatcgcc ctggtgctgg
     3301 tcgccgaccc gatcggcgac acactggtcg ccaacggg
//
LOCUS       DATGUK010000027         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_32438,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000027 DATGUK010000000
VERSION     DATGUK010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32438"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2450)
                     /locus_tag="VK103_00430"
     CDS             complement(<1..>2450)
                     /locus_tag="VK103_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81971.1"
                     /translation="ATGQERYRRIDPTLSYTVDGEQQSQRLSSVPLSTAADHPENVLD
                     TAAFTLPDGATDVSLDYLQSDGEAPVLSWFAIATGDAEEVIDDAVATDPADAVEVTID
                     AADIAADNVNGLTFKGFGVLSANSTSAVLMDYKSEHPEAYARLLRTLFGGEHPIMNQV
                     KIEMGNDRNNSTGPDPATMRTADEPANVTRDPGFQLAADARTVNPDLKVSILRWNAPA
                     WVDGNDDIYTWYKNTVLAAYREYGYMVDYVNPGVNEHEADLTWSQEFAERVRTDTTGY
                     VSDDPELAGFRAGEAELFQQIEVVVSDEVGVGTFGEEMVADADLREAVPVAAYHYNTN
                     DDASGNFTRLAEEYDLEVWNSEAQATMSNTSFRPNNNTEDPTVAGTGIGGHISALEMA
                     NTVVKGFVNSRRTHFIYQPAIGSFYEGGQYSFKELVSARDPWSGWMHYDGGLAVLQHF
                     SSFAETGWENPDNTAGTWRLVPESSASGASGTNPVNGRNGLPNYLTMAAPDGSDFSTV
                     IVNDSEYPLSYRISPTGFDLDEPLAVWETRAADEGEAFDADYKQHVADVTATDGSYLV
                     QVAPYSISTVTSLDVADDPQWSEPLPVEGERTVLDLDPASGTLWQDGFDYTDRTVAQI
                     TADGQVGTETESFVDSRGGPSGVIPMYTWDRNGAFEAYPDEDGWVLRQQLDAEATGVG
                     GAWNGGDPITAVGDRRWSNYRASVDVRFERGTDEGNYAAVGVRSSGGDNSQLLADTPY
                     ALRLAHDGTWQFLRMGTVTESGSIDGAGTGWHQLSLQAAGSQLTGWIDDEQVFTWTDP
                     DPIRSGWVDLASGFHFTQ"
BASE COUNT          323 a          808 c          889 g          430 t
ORIGIN      
        1 aactgggtga agtggaaccc gctggccagg tccacccagc cggagcggat cggatccggg
       61 tcggtccagg tgaagacctg ctcgtcgtcg atccacccgg tcagctgcga cccggcggcc
      121 tgcagggaca gctggtgcca gccggtgccg gcgccgtcga tgctgccgga ctcggtcacg
      181 gtacccatcc gcaggaactg ccaggtgccg tcgtgggcga ggcgcagcgc gtagggggtg
      241 tcggccagga gctggctgtt gtccccgccg ctggaccgca cgccgacggc cgcgtagttg
      301 ccttcgtcgg tgccccgctc gaaacggacg tccacgctgg cccggtagtt cgaccagcgt
      361 cggtcgccga ccgcggtgat cgggtcgcca ccgttccagg cgccgcccac gccggtggcc
      421 tcggcatcca gctgctggcg cagcacccag ccgtcctcgt cggggtaggc ctcgaacgcc
      481 ccgttccggt cccaggtgta catcgggatc acgccggagg gtccgccgcg ggagtccacg
      541 aacgactccg tctcggtgcc gacctgcccg tcagcggtga tctgcgcaac ggtgcggtcg
      601 gtgtagtcga acccgtcctg ccagagcgtg cccgaggccg ggtcgaggtc gagcacggtg
      661 cgctccccct ccaccggcag cggctcgctc cactgcgggt cgtcggccac gtccagggag
      721 gtgacggtgc tgatcgagta cggcgccacc tgcaccaggt aggagccgtc ggtggcggtg
      781 acgtcggcga cgtgctgctt gtagtcggcg tcgaacgcct cgccctcgtc cgccgcccgg
      841 gtctcccaga cggccagcgg ctcgtccagg tcgaaaccgg tcggggagat ccggtagctg
      901 agcgggtact cggagtcgtt gacgatcacc gtggagaagt ccgacccgtc cggggcggcc
      961 atggtgaggt agttcggtag cccgttgcgt ccgttcaccg ggttcgtgcc gctggccccg
     1021 gaggcgctgg actccgggac gagccgccag gtgcccgcag tgttgtccgg gttctcccag
     1081 ccggtctcgg cgaagctgga gaagtgctgc agcacggcca gcccgccgtc gtagtgcatc
     1141 cagcctgacc acgggtcacg ggcgctgacc agctccttga aggagtactg cccgccttcg
     1201 tagaaggagc cgatcgccgg ctggtagatg aagtgcgtcc ggcgggagtt cacgaagccc
     1261 ttgaccacgg tgttggccat ctccagggcg gagatgtgcc caccgatccc ggtgccggcc
     1321 acggtggggt cctcggtgtt gttgttcggc cggaaggagg tgttgctcat cgtggcctgc
     1381 gcctcggagt tccacacctc caggtcgtac tcctcggcga ggcgggtgaa gttgccgctg
     1441 gcgtcgtcgt tggtgttgta gtggtacgcc gccaccggta ccgcctcgcg caggtcggcg
     1501 tcggccacca tctcctcacc gaaggtgccc acaccgacct cgtcggagac gaccacctcg
     1561 atctgctgga acagctcggc ctcaccggct cggaagcctg ccagctccgg gtcgtcgctg
     1621 acgtaaccgg tggtgtcggt gcgcacccgc tcggcgaact cctgggacca ggtcaggtcg
     1681 gcctcgtgct cgttcacgcc ggggttgacg tagtcgacca tgtagccgta ctcgcggtag
     1741 gcggccagga cggtgttctt gtaccaggtg tagatgtcgt cgttgccgtc cacccacgcg
     1801 ggggcgttcc accgcaggat ggagaccttg aggtcggggt tgacggtgcg ggcgtccgcg
     1861 gcgagctgga agccggggtc gcgggtgacg ttcgccggct cgtctgcggt gcgcatcgtg
     1921 gccgggtcgg gaccggtgga gttgttccgg tcgttgccca tctcgatctt gacctggttc
     1981 atgatcgggt gctcgccgcc gaagagcgtc cgcagcagcc gggcgtaggc ctcggggtgc
     2041 tcggacttgt agtccatcag cacagcgctg gtgctgttcg cgctgagcac gccgaagccc
     2101 ttgaacgtca gtccgttgac gttgtctgcg gcgatgtcgg cggcgtcgat ggtcacctcg
     2161 accgcatcgg cggggtcggt ggccacggcg tcgtcgatca cctcctcggc atcgccggtg
     2221 gcgatcgcga accagctcag caccggagcc tcgccgtcgg actgcaggta gtcgaggctg
     2281 acgtcggtgg caccgtcggg cagcgtgaac gccgcggtgt cgagcacgtt ctccgggtgg
     2341 tcggcggcag tggacagcgg caccgaggac agccgctggg actgctgctc gccgtcgacg
     2401 gtgtagctga gggtcgggtc gatccgccgg taccgttcct gcccggtggc
//
LOCUS       DATGUK010000028         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_961993,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000028 DATGUK010000000
VERSION     DATGUK010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961993"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VK103_00435"
     CDS             complement(<1..458)
                     /locus_tag="VK103_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HLQ81972.1"
                     /translation="MTDQSAPPTPHSSVAAVVVLAAGAGTRMRSAKSKLLHQVAGRSL
                     VSHAIRSGESIAPHRMIVVVGMLRDQVEAHLAEVAPEVTVVGQSEDGYGTGHAMRCAM
                     SALTDVDGEVIVLGGDVPMLQPETLAAMVESHRAERAAITILSARVPDPTG"
     gene            644..1546
                     /locus_tag="VK103_00440"
     CDS             644..1546
                     /locus_tag="VK103_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81973.1"
                     /translation="MRSDSDDERATTAGPPLWALPLAMLLVGLLAVAAGAVAALTGGW
                     FGGLGAERTVRITGTGLLQQRPEGPIVLCVGAVGDAEPATCGTTVAVTGVSWRSVPEV
                     ATHAGTTEGWATLTGVWSDGVLHVDTVEEPQARTVLPAETFPQQLCLDPGGSSSVTAP
                     GATPDPTLLEQLPGFQGYWRTDDGGTASGGDGTVLVHNVAVRGNVDQARQQLRQSFDG
                     PLCVGTIPGMTQAEAQDAMARLTPQFADLGLLSGHPSLSGGWSGLDLEVFVVTPEVQQ
                     EIYEILGPELARWTTVRGQFRVVG"
     gene            complement(1551..2153)
                     /locus_tag="VK103_00445"
     CDS             complement(1551..2153)
                     /locus_tag="VK103_00445"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLQ81974.1"
                     /translation="MAFRRIAVTEGRTRRPRLIHLNGPAGIGKSTLAERWVAEHPLTL
                     NCDVDVLRGLIGSGQRLPEAMALSRTTAIALITAHLTEGYDVVLPQLVARADQLERFA
                     GAAAGAGADYVEVMLLEASGDPDTAVARFHRRGDDGWHREVRDLVAAQGGDAMLRSRY
                     AALVDLVRQRPEAIVLSSVEGDPDATYRQLLDAVDGPRST"
     gene            complement(2276..>2529)
                     /locus_tag="VK103_00450"
     CDS             complement(2276..>2529)
                     /locus_tag="VK103_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81975.1"
                     /translation="SGVDADVTGRFADEHTEAMITGLPDDVRGVRVRTVEGRFAVLAN
                     LGDEPATIRLGTPGQSLVDDAVSDRVELAPLRGTVLAID"
BASE COUNT          357 a          889 c          917 g          366 t
ORIGIN      
        1 ccggtgggat cggggacgcg cgcgctcagg atggtgatcg cggcccgttc ggcgcggtgc
       61 gactccacca ttgcggcgag tgtctccggc tgcagcatcg gcacgtcacc gccgaggacg
      121 atgacctcgc cgtcgacgtc ggtgagtgcg ctcatcgcgc agcgcatcgc gtgcccggtg
      181 ccgtacccgt cctcggactg gccgacgacg gtgacctcgg gggcgacctc ggccaggtgg
      241 gcctccacct ggtcacgcag catgccgacc acgacgatca tccggtgcgg tgcgatcgac
      301 tccccggacc ggatcgcatg gctgaccaac gaccgtccgg cgacctggtg gagcagtttt
      361 gacttcgccg acctcatgcg tgtgccggcc ccggcggcga gcaccaccac ggcagcgacg
      421 gacgagtgcg gcgtcggtgg tgccgactgg tcggtcacgc gatcctccct gggctgggat
      481 cctcccgtca cgtgggcccg gcactgccgc gcccacgcgg accgtaccac ccacttgggg
      541 ttgaggggca tttgagggga agatggggtg caacccggga cggtttgtcg gacgtgtccg
      601 ctatggatgt gtcatggagg gacggcccgg ccgaaggaga gcgatgcgtt cggacagcga
      661 cgacgagcgg gcgacgaccg ccggcccacc cctgtgggcg ctcccgctgg ccatgctgct
      721 ggtgggacta ctggcggtcg cggcgggggc cgttgctgcg ctcacgggcg gctggttcgg
      781 cgggctgggc gccgaacgca ccgtacggat caccggcacc gggctgctgc agcagcgtcc
      841 ggagggtccg atcgtgctgt gcgtcggagc cgtgggtgat gccgagccgg cgacctgtgg
      901 gacgacggtc gcggtgaccg gcgtcagttg gcgttccgtg ccggaggtgg ccacccacgc
      961 cgggacgacc gagggttggg cgacgctgac cggggtctgg tccgacgggg tcctgcacgt
     1021 cgacaccgtc gaggagccgc aggcgcgcac cgtgctgccg gccgagacgt ttccgcagca
     1081 gctgtgcctc gacccgggcg ggtccagctc ggtcaccgca cccggggcca cgccggaccc
     1141 gaccctgctc gaacagctcc ccgggttcca gggctattgg cgcaccgacg acggcggcac
     1201 cgcctccggt ggtgatggca cggtgctggt ccacaacgtg gcggtccgtg gcaacgtcga
     1261 ccaggcgcgg cagcagctcc ggcagagctt cgacggcccg ctgtgcgtcg gcaccatccc
     1321 cggcatgacg caggccgagg cccaggacgc gatggcccgg ctgacgccgc agttcgccga
     1381 ccttggcctg ctcagtggac acccgagcct gtcgggtggc tggtcgggtc tcgacctgga
     1441 ggtgttcgtg gtcaccccgg aggtccaaca ggagatctac gagatcctgg gcccggagct
     1501 ggcgcgctgg acgacggtac ggggacagtt ccgggtggtg ggctgacggg tcaggtggac
     1561 cgggggccgt cgaccgcgtc gagcagctgc cggtaagtgg cgtccggatc gccctcgacg
     1621 ctggacagca cgatcgcctc ggggcgttgc cgcaccaggt cgaccagggc ggcataccgg
     1681 ctgcgcagca tcgcatcgcc cccctgggcg gcgacgaggt cgcggacctc ccggtgccac
     1741 ccgtcgtcac cgcgacggtg gaagcgggcc accgccgtgt ccgggtcgcc cgaggcctcc
     1801 agcaacatca cctccacgta gtcggccccg gcaccggctg cggcaccggc gaatcgctcg
     1861 agctggtcgg cccgggccac cagctgcggc agcaccacgt cgtatccctc ggtcaggtgg
     1921 gcggtgatca gtgcgatggc ggtggtccgg ctcaacgcca tcgcctccgg cagacgttgg
     1981 ccactgccga tcaggccccg caacacgtcg acgtcgcagt tcagcgtgag cgggtgctcg
     2041 gcaacccacc gctcggcgag ggtcgacttg ccgatgccgg cgggcccgtt gaggtggatc
     2101 agccggggtc gccgggtgcg cccctcggtc acggcaatcc gacgaaacgc catggcggca
     2161 acgtacaacc agcccggatc gcatgcatcg tggcctccgg gccgacaccg cccaccgacc
     2221 tgtccaggta ccgtggtcgt gcagcagccc gatcgcctga tcccgcggca cccgatcagt
     2281 cgatcgccag caccgtccca cgcagcggcg ccagctcgac ccggtcgctc accgcgtcgt
     2341 cgaccaggga ctgcccgggg gtgccgagcc ggatcgtcgc gggttcatca ccgagatttg
     2401 ccagcacggc gaagcggccc tccacggtcc ggacacgaac tccacgcaca tcatcgggca
     2461 ggcccgtgat cattgcctca gtgtgctcgt cggcaaatcg gccggtcacg tccgcgtcga
     2521 cgccactga
//
LOCUS       DATGUK010000029         5227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_497830,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000029 DATGUK010000000
VERSION     DATGUK010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497830"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VK103_00455"
     CDS             complement(<1..394)
                     /locus_tag="VK103_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HLQ81976.1"
                     /translation="MDEPDRERDGDATRDGDAGAQSRAERQRLISLDPESGTVDADVG
                     SSGRGLYWLLRRIVLGPVLRRLFRPIEEGVHNVPTAGPAILAANHLSFADWIFLPLAL
                     NRRVTFVAKSDYFTGSGVKGAAQRTFFAG"
     gene            complement(381..1163)
                     /locus_tag="VK103_00460"
     CDS             complement(381..1163)
                     /locus_tag="VK103_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLQ81977.1"
                     /translation="MYEICRRLVWLWFKLTARPQVFHAERIPLTGPAIIAGNHMSAVE
                     TVLIPSIVHRKLGHVAKSELFTSRKPFHRLLGWFIHWAGARPMDRSGGRMSASSMDDM
                     LTHLRKGGAAVVFPEGTRAPDERMYKGKTGVARLALAADVPVIPVGIVNTRKRKVGWW
                     PWPIMWKPEVHWGEPLDFSQWAGCVDDRGVVRHVTDEVMAAIQQLTGQTYVDVYAASL
                     RSATKDGRELAAPELPRPGYGKPVPQLPEQSGQDAGEHDGRA"
     gene            complement(1189..2931)
                     /locus_tag="VK103_00465"
     CDS             complement(1189..2931)
                     /locus_tag="VK103_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81978.1"
                     /translation="MSVRALVSAVGEVVSSSARVAFIRPVQAGRVQIRVWPTQMRVTI
                     IATYVVYGAVVLSVVLAPLIVARGPGYNDAGLAPWGAVPVTVGLWASMVLLFLASLTL
                     HPLWRVGSWVLLAIPHLLTAGTVALIVPAPGVGRPLLSLLVAGTSWLLGTVLLVTIVA
                     IGSGRPLRTGVVVLTVVALTLTYLVPQLVAMQAPMVGSVGPAITAIIMTFLAMPLAVA
                     AGTAFVQITINLTTATMVSVRAHAPTWLWLVVGPVAALATLVVAVTVPRDADSGGVPI
                     TIGFTVLSAVTSMVALVVAHRRAKRMHVEHGAAMSYPRPLVLSEILADVSMVLGILLG
                     SWGIPLLLGFVMPLPALLRERPAQISDLLMAAAALVLTVRAARAGRTAAAMLLPPVAL
                     MGLYSSLRGWLELPTLSGSVSSATLTVGLAGVAILWARRGGGRHRWFVLSLGAVILLV
                     FPFREAIAEPLEAVLGPSQVGVLLVGLVWLLLTEAEFTHRGSPRFGRTARILVFLAYA
                     LFTAAMMAAVAYGADGSVIRVAHLGELADIGDAIIGYAVAPAVIVGLVALGHRDIDPV
                     SRSPEPAGHRKVRS"
     gene            3015..3884
                     /locus_tag="VK103_00470"
     CDS             3015..3884
                     /locus_tag="VK103_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLQ81979.1"
                     /translation="MTSLQGPTTPEIVPGAESWQGVPADGPVRGAVVLSHGFTGSPFS
                     LRGWGEQLVAAGYEVSVPRLAGHGTTWQEMNRTDWTDWYGTVEAEVLRLSERHDRVGV
                     GALSMGGSLALRIAQQHPDRVTGLALVNPSVGSADPKMRFVPLLSRFVPSIEGIADDI
                     AKPDVTERAYPRTPLRAVASMMRLWRTVRADLDQVRCPILLFRSLVDHVVDPSSAKLI
                     MEQVSSESVTERLLQRSFHVATLDFEADDIVAESIEFYGSLLAAPADGPVRDERATSR
                     LPPTTAGTSTGSG"
     gene            3890..4522
                     /locus_tag="VK103_00475"
     CDS             3890..4522
                     /locus_tag="VK103_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81980.1"
                     /translation="MTAREPSDAEVEAAFNDIVARLTGRDGLDDSWADQHPLFADDAG
                     SSGPNEVDESGTDWSGTDWSGTDRSGAGRPGTDGPDGPTDDGPVLRGEVLDPAPVPGP
                     DDDPVDEQEDEPLGWRVHRPPDDEDLDELDLDELPRTPLPPWRPSPTAISALVLITAA
                     VIVTVLMIAQVWLPWWAGWGALGAFLAGMGLLFSRLPRDRDEDPDDGAVV"
     gene            complement(4591..4977)
                     /locus_tag="VK103_00480"
     CDS             complement(4591..4977)
                     /locus_tag="VK103_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81981.1"
                     /translation="MVSAHYGGWIEQGWDQARTRSPLEVVAGMTESGMVQLRARDLHV
                     IARTIVLAVLAVPAGLGLSVVGFLGWCVGPLTIITGLVGKHVRRGWASWVIPIGVGLT
                     LGALAYIAIGLLMPDGPSSGSGSTHH"
BASE COUNT          873 a         1824 c         1830 g          700 t
ORIGIN      
        1 tgccggcgaa gaaggtgcgc tgggcagccc ccttcacccc ggaaccggtg aagtagtccg
       61 acttcgccac gaaggtgacc cgacggttca acgccagcgg taggaagatc cagtccgcga
      121 aggacaggtg attggcggcg aggatcgcgg gcccggcggt cgggacgttg tgcacgccct
      181 cctcgatcgg acggaacagt cgccgcagca ccggaccgag gacgatgcgg cgcagcaacc
      241 agtacagccc ccgaccgctg ctgcccacat cggcgtcgac ggtgccggac tccggatcga
      301 gcgagatcag ccgttgccgc tcggcgcggg actgtgcccc ggcgtcaccg tcacgcgtgg
      361 cgtcaccgtc acgttcgcgg tcaggctcgt ccatcgtgct ccccggcgtc ctgaccggac
      421 tgctccggca gctgcgggac cggtttgccg tacccgggcc gaggaagttc gggggccgcg
      481 agctcacgac catccttggt ggccgaccgc agactggctg cgtacacgtc gacataggtc
      541 tgcccggtga gctgctggat ggcggccatc acctcatcgg tcacatggcg cacgacgcca
      601 cggtcatcga cgcaaccggc ccactggctg aagtccagcg gctcacccca gtgcacctcc
      661 ggcttccaca tgatcggcca cggccaccac ccgaccttgc gcttgcgggt gttgacgatg
      721 ccgaccggga tgaccgggac gtcggcggcc aacgccagcc gggcgacacc ggtcttgccc
      781 ttgtacatcc gctcgtccgg tgcgcgggtg ccctccggga acaccaccgc agcgccgccc
      841 ttgcgcaggt gggtgagcat gtcgtccatc gaggaggccg acattcgacc accggaccgg
      901 tccatcggtc gggcaccggc ccagtggatg aaccagccga gcagccggtg gaacggcttg
      961 cggctggtga acagctcgct cttggcgacg tggcccagct tccggtgcac gatcgacggg
     1021 atcagcacgg tctcgaccgc cgacatgtgg ttgcccgcaa tgatcgctgg acccgtcagg
     1081 gggatgcgct cagcgtggaa cacctgcggt cgagcggtga gcttgaacca cagccacacc
     1141 agacggcgac agatctcgta catcctcgtg gtggcctctc ctccggtttc aagaacgaac
     1201 cttacggtgc cccgcaggct cgggcgagcg gctgaccggg tcgatgtcgc ggtgcccgag
     1261 cgcgacgagg ccgacgatga cggccggggc gacggcatag ccgatgatcg cgtcgccgat
     1321 gtcggccaac tcccccaggt gcgcgacgcg gatcacgctg ccgtcggcgc cgtaggcgac
     1381 cgccgccatc attgccgcgg tgaacagcgc gtaggccagg aacaccagga tgcgggccgt
     1441 acggccgaac cgtgggctac cgcggtgggt gaactcggcc tcggtcagca gcagccacac
     1501 caggccgacg agcagcacgc ccacctggga ggggccgagc acggcctcga gcggttcagc
     1561 gatcgcctcc cggaacggga acaccagcag gatcacagcg cccaggctca gtacgaacca
     1621 gcggtgccgg ccaccacccc ggcgggccca caggatcgcc accccggcca ggccgacggt
     1681 cagcgtcgcc gagctcaccg agccggacag ggtcggcagc tccaaccagc cccgcaggct
     1741 gctgtacagc cccatcaggg ccaccggggg cagcagcatc gccgcggcgg tacggccggc
     1801 ccgggccgca cgcaccgtca ggaccagcgc ggccgcagcc atcaggaggt cggagatctg
     1861 cgccggtcgt tcccgcaaca gcgcgggcag cggcatgacg aaaccgagga gcagcgggat
     1921 cccccagctg ccgagcagga tcccgagcac catcgacacg tcggccagga tctcgctgag
     1981 caccaatgga cgcgggtacg acatggcggc accgtgttcg acgtgcatgc gcttcgcccg
     2041 ccggtgcgcg accaccagtg caaccatgct cgtgaccgcg ctgagcacgg tgaacccgat
     2101 cgtgatgggc accccgccgg agtcggcatc acgcgggaca gtgacggcca ccacgagcgt
     2161 ggccagggcg gcgaccgggc cgacgaccaa ccacagccac gtcggcgcgt gcgcacgcac
     2221 cgacaccatc gtcgcggtcg tcaggttgat cgtgatctgg acgaaggcgg tgccggccgc
     2281 cacggccagc ggcatcgcca ggaacgtcat gatgatggcc gtgatcgccg ggccgacgga
     2341 gccgaccatc ggtgcctgca tggccaccag ttgtggcacc agataggtca gtgtcagtgc
     2401 gacgacggtc agcaccacga caccggtgcg cagcggccgc cccgatccga tcgcgacgat
     2461 cgtcaccagc agcaccgtgc cgagcagcca cgaggtgccg gccacgagca aggacagcaa
     2521 cggccgaccg acaccgggcg ccggcacgat cagggcaacg gtgccagccg tcagcagatg
     2581 cggtatcgcc aacagcaccc aactgccgac ccgccacagc gggtgcagag tgagcgaggc
     2641 caggaacagc agcaccatgc tcgcccacag gccgacggtg accgggacgg caccccacgg
     2701 cgccaggccg gcatcgttgt accccggccc ccgcgccaca atcagagggg ccaacacgac
     2761 gctcagcacc acagcgccgt acaccacgta ggttgcgatg atcgtcacac gcatctgggt
     2821 cggccagacc cggatctgca cccgacccgc ctggaccggc cggatgaacg ccacccgggc
     2881 ggagctgctg acgacctctc cgacggcgga gaccagggca cggacggaca cgccgaccag
     2941 cgaaccacag cgtgccggga ggcgcacaac gtggcacgct gtctgcactg gccccgggtc
     3001 ctaggattgg tggcatgacg agcctgcagg gccccacgac cccggagatc gtcccgggcg
     3061 ccgagtcgtg gcagggcgtg cccgccgacg gtccggtccg tggtgcggtc gtgctgagcc
     3121 acggcttcac gggttcgccg ttctcgctgc gcggctgggg cgaacagctg gtggccgccg
     3181 gctatgaggt ctcggtgccc cgactcgccg gccacggcac cacgtggcag gagatgaacc
     3241 ggaccgactg gaccgactgg tacggcaccg tcgaggccga ggtgctgcgg ttgtcggaac
     3301 gccacgaccg cgtcggggtg ggggcgctgt cgatgggcgg ctcgctggcc ctgcggatcg
     3361 cgcagcaaca tcctgaccgg gtgaccgggc tggcactggt caacccgtcg gtcggcagcg
     3421 ccgatccgaa gatgcgattt gtgccgctgc tgtcccggtt cgtgccctcc atcgagggga
     3481 tcgccgacga catcgccaag ccggatgtca ctgagcgggc ctatccgcgc acgcccctgc
     3541 gagcggtcgc gtcgatgatg cggctgtggc ggaccgtccg ggctgacctg gaccaggtgc
     3601 gctgcccgat cctgctgttc cggtcgctgg tcgaccacgt cgtcgacccc tcctcggcaa
     3661 aactgatcat ggaacaggtc tcgtcggagt cggtgaccga acgcctgctg cagcgcagct
     3721 tccatgtggc gaccctggac ttcgaggccg acgacatcgt ggccgagtcg atcgagttct
     3781 acggcagcct gctggctgcc ccggccgatg gcccggtacg ggacgagcgt gcgacgtcgc
     3841 ggcttccgcc gaccaccgcg gggacctcca cgggatcggg ttgactgtca tgaccgcccg
     3901 ggaacccagc gacgccgagg tcgaggccgc cttcaacgac atcgtcgccc gcctcaccgg
     3961 acgtgacggc ctggacgaca gctgggccga tcagcacccg ctgttcgccg acgatgccgg
     4021 gtcgtcgggg ccgaacgagg tggacgagtc cggcacggac tggtccggca cggactggtc
     4081 cggcacggac cggtcgggcg ccggccggcc cggcactgac gggccggacg gcccgacgga
     4141 tgacggcccg gtgctgcggg gagaggtgct cgatcccgcc cccgtccccg gaccggacga
     4201 cgaccctgtc gatgagcagg aggacgagcc gctcggttgg cgggtgcatc ggccgccgga
     4261 tgacgaggac ctcgacgagc tggacctcga cgagttgccg cgaacccccc tgccgccgtg
     4321 gcggccgtcg ccgacggcga tctcggcgct ggtgctgatc acggccgccg tcatcgtgac
     4381 cgtgctgatg attgcgcagg tgtggctgcc gtggtgggcg ggttggggcg ccctgggcgc
     4441 gttcctggcc gggatgggcc tgctgttcag ccggctgccc cgggatcgtg acgaggaccc
     4501 cgacgacggc gcagtcgtct gaggcagcga gccgtcgccg gcgcgtccgg gcttgcccac
     4561 gcactgcgac gttgcgtgcg gccgtcaagg tcagtggtgg gtcgatccgg agccggacga
     4621 tggcccgtcc ggcatgagca gaccgatggc aatgtatgcg agcgctccga gagtcagtcc
     4681 gacaccgatc gggatgaccc acgacgccca accacgacgc acgtgttttc cgacgaggcc
     4741 ggtgatgatg gtcaaagggc cgacgcacca gccgaggaaa ccgacaacgg acaggccgag
     4801 accagcaggg acggcaagca cggccaacac aatcgttctc gcgatgacgt gcaagtcccg
     4861 tgcgcgcagc tggaccatgc ctgattcagt catgccggcc accacctcca gcgggcttcg
     4921 ggtgcgcgct tggtcccagc cctgttcgat ccatccaccg tagtgcgcgg agaccatgtt
     4981 gcgatcgcgt ggggtggcga gatcgcgatt gcgcagagca tgatgccgtc ctggcgggtc
     5041 agggtgccgg tcgtcgcgga cgggccgggt cgcgtcaccg tctgaagatc cccaggcgtt
     5101 cccagcagcg gacgggcgcg gatgctccgg gcatgcccgt gcactccgac ggctcggtcg
     5161 accgtgaccg tcggagcgca atgagcacgc agcagtgagt cgggcggtag tggacggaag
     5221 cgggctc
//
LOCUS       DATGUK010000030         4373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_498980,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000030 DATGUK010000000
VERSION     DATGUK010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498980"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VK103_00485"
     CDS             <1..893
                     /locus_tag="VK103_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HLQ81982.1"
                     /translation="PVPEVLAGLAGEGSRGTLSILWDVRLPRVGLALLIGANLAASGT
                     LLQAVMHNPLADPGLTGVSSGAAVAVLFVILTAPQWTHLIPPVALVGGVVATVMVFTF
                     AWQRHNGFTPLRVILAGVAVNSIFGGITGMLSILHSDRLPAALQWLNGSLAGTGMDDL
                     GTLLPYSLLGWIAALVCIRPANILRLGEQTAMGIGLNVTLARLLLCGVAVHLAAISVS
                     TVGLIGFVGLVVPHITRMLVGSNYLVRIPFGLALGAVVLVAADTLGRTVISPLEVPAG
                     IVMAVVGGPYFLYLMRRGGT"
     gene            893..1675
                     /locus_tag="VK103_00490"
     CDS             893..1675
                     /locus_tag="VK103_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLQ81983.1"
                     /translation="MLQVRDLRVGYHGTPVVHGVDLTVAEGSVTSIIGPNGSGKSTVL
                     KAVTRTVPSTGSVRLAGEDVGVLSRRRMARLVSMVGQVRETPADMTVTELVGLGRSPH
                     RPWFHPLGARDRRIVAEVIEQAGLQQFRDRTVATLSGGEAQRAWIAMALAQRPRLLLL
                     DEPTTYLDIAHQLEVLDLVRSINADLGLTVVMVLHDLNHAGFYSDHLVVLDHGRVVTT
                     GAPSAVLSADLVEQVYGLRVEIDHPRGDDWPRIHTLPTGSLL"
     gene            1689..2243
                     /locus_tag="VK103_00495"
     CDS             1689..2243
                     /locus_tag="VK103_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLQ81984.1"
                     /translation="MSVIDVDTERAGYHDFLDSRRTMVLSTTDADGHPFISYAPFVVH
                     DGSFHVYVSRIAEHYWHLVNLDRVSGLLIADEKDSPNLFARHRVRFECAVTRLDDPDE
                     GTDTDPHEDVFALFAERFNASLITLLRTLDFSLFRLTPLSGRYVVGFGKAFDVDLAGS
                     RFDHVVVDKRQAEIREQDRRGRMG"
     gene            complement(2253..4181)
                     /gene="dnaG"
                     /locus_tag="VK103_00500"
     CDS             complement(2253..4181)
                     /gene="dnaG"
                     /locus_tag="VK103_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HLQ81985.1"
                     /translation="MTGAPAAPWAPTYTAAVAGRINDEDVAAVRERARIDEVIGGHLQ
                     LRNAGGGSKKGLCPFHDEKTPSFQVTPAKGLFYCFGCGEGGDVITFVQKIDNLTFTEA
                     VQRLADRVGVQLRFTEGGGPAVAPGLRMRLLEAHQLAAEFFAEHLAGPDALVARQFLA
                     ERGFDRDAAERFGLGYAPRGGRELADHLRAKGFSHQELVAGGLVRERGWDFFQGRLLW
                     PIRDSATSVLGFGARRLHDDDRMPAKYLNTPETPLYKKSQVLYGLDLARTAVGKKSQA
                     VIVEGYTDVMACHLAGVDTAVASCGTAFGHEHARLLRRLMGDHDAFHGEVIFTFDGDE
                     AGQNAALKVFAEDANFVSQTYVAVEPTGLDPCDLRLQQGEAAVRELVGRREPLYRFVM
                     GNIVGRYDLDRVEGRVQALRAAAPLVASVRDQSMVSGYIRELAALIGLDIDEVRAQVL
                     RAASRPRDSARAQAPPTPPEPESDGGPGATGPALPDPRDRDLVVEREAAKLLLQVPQS
                     FDADFHGLTEDDFHHPAYRAVFTAARTVRAVAAPGDGWSDRVRAELPAPELQQLGVAL
                     TVEPLSTNREPDTAFVRACAAQLRLIHLMGRITDLKSKLQRTNPVSDTARYNQMFSVL
                     LGLESDRARLKQLAVGEG"
BASE COUNT          628 a         1579 c         1503 g          663 t
ORIGIN      
        1 tcccggtgcc cgaggtgttg gccgggctgg ccggcgaggg cagccgcggc accctgagca
       61 tcctgtggga cgtgcggctg ccgcgggtcg ggctggccct gctgatcggg gccaacctgg
      121 ccgcatcggg gaccctgctg caggcggtga tgcacaaccc gctcgccgac ccgggcctca
      181 ccggcgtgtc cagcggagcg gcggtcgcgg tgctcttcgt gatcctcact gcgccgcagt
      241 ggacgcacct gatcccgccg gtggccctgg tcggtggggt cgtcgccacc gtgatggtgt
      301 tcaccttcgc ctggcaacgc cacaacgggt tcacgccgtt gcgggtgatc ctcgccgggg
      361 tcgcggtgaa ctcgatcttc ggcggcatca ccgggatgtt gtcgatcctg cacagcgatc
      421 ggctgcccgc cgccctgcag tggctcaacg gcagcctggc gggcaccggc atggacgacc
      481 tgggcaccct gctgccgtac tcgctgctcg gctggatcgc tgcgctggtg tgcatccggc
      541 ccgccaacat cctgcggctg ggcgagcaga cggcgatggg catcgggctc aacgtgacgc
      601 tggcacggct gctgctgtgc ggggtggcgg tccacctggc cgcgatctcg gtgtcgacgg
      661 tcgggctgat cggcttcgtc gggctggtgg tgccccacat cacgcggatg ctggtcggct
      721 ccaactacct ggtacggatc cccttcggcc tggcgctcgg tgctgtcgtg ctggtggccg
      781 ccgacaccct cggtcggacg gtgatctcgc cgttggaggt ccccgccggg atcgtgatgg
      841 cggtggtcgg tgggccgtac ttcctctacc tgatgcgccg agggggcacc tgatgctgca
      901 ggtgcgcgac ctgcgggtcg gctaccacgg cacccctgtg gtgcacggcg tcgacctgac
      961 cgtggccgag ggctcggtca cctcgatcat cggcccgaac gggtcgggca agtcgaccgt
     1021 gctcaaggcg gtcacccgta cggtcccgtc gaccgggtcg gtccggctgg ccggggagga
     1081 cgtcggcgtg ctgtcccggc gacggatggc ccggctggtg agcatggtcg gccaggtccg
     1141 cgagaccccg gccgacatga ccgtcaccga actcgtcggg ctcggacggt ccccgcaccg
     1201 accgtggttc cacccgctcg gcgcacggga ccggcgcatc gtcgccgagg tcatcgaaca
     1261 ggccggcctg cagcagttcc gcgaccggac cgtcgccacg ctgtccggcg gtgaggcgca
     1321 acgcgcctgg atcgcgatgg cgctggccca acgtccccgg ctgctgctgc tcgacgagcc
     1381 gaccacctac ctcgacatcg cccaccagct ggaggtcctc gacctggtgc gcagcatcaa
     1441 cgccgacctc gggctgaccg tggtgatggt gctgcacgac ctcaaccacg ccggcttcta
     1501 cagcgaccac ctggtggtgc tggaccacgg ccgggtggtc accaccggtg cgccgtcggc
     1561 ggtgctgtcg gccgacctgg tcgagcaggt ctacggtctg cgggtcgaga tcgaccaccc
     1621 acgcggggac gactggcccc gcatccacac cctgcccacc ggatccctac tgtgaaagga
     1681 acccctccat gtccgtgatc gacgtcgaca ccgaacgcgc cggctaccac gacttcctcg
     1741 acagccgtcg cacgatggtg ctcagcacca ccgacgccga cggccacccg ttcatctcct
     1801 acgcaccgtt cgtggtgcac gacggcagct tccacgtgta cgtgagccgg atcgccgagc
     1861 actactggca cctggtcaac cttgatcggg tcagcggact gttgatcgcc gacgagaagg
     1921 actcgcccaa cctgttcgcc cggcaccgcg tgcggttcga gtgcgcggtc acccgactgg
     1981 acgacccgga cgagggcacc gacaccgacc cgcacgagga tgtcttcgcc ctgttcgccg
     2041 agcggttcaa cgccagcctg atcacgctgt tgcgcacgct ggacttctcg ctgttccggc
     2101 tcacaccgct gtcgggtcgc tatgtggtcg gcttcggcaa ggcattcgac gtcgacctgg
     2161 cgggcagccg gttcgaccac gtggtggtcg acaagcggca ggccgagatc cgcgagcagg
     2221 accgtcgggg acggatgggc tgagcgcggc gctcacccct cccccacggc gagctgcttg
     2281 agccgggccc ggtcggactc cagtcccagc agcaccgaga acatctggtt gtagcgggcc
     2341 gtgtcgctga ccgggttggt gcgctgcagc ttcgacttca ggtcggtgat ccgtcccatc
     2401 aggtggatca gccgcaactg cgcggcacag gcacggacga aggcggtgtc gggctcccgg
     2461 ttggtgctga gtggctcgac ggtgagggcc accccgagct gctgcagctc cggagccggc
     2521 agctcggccc ggacccggtc ggaccagccg tcgccggggg ctgcgacggc ccggaccgtc
     2581 cgggccgcgg tgaacaccgc acggtaggcg gggtggtgga agtcgtcctc ggtcaggccg
     2641 tggaagtcgg cgtcgaacga ctgcggcacc tgcaacagca gcttggcggc ctcccgctcc
     2701 accaccaggt cacggtcgcg cggatccggc agcgccggcc ccgtcgcgcc gggcccgccg
     2761 tcggactccg gctccggcgg tgtcggcggg gcctgtgccc gggcgctgtc ccgcggccgc
     2821 gaggcagccc gcagcacctg cgcccgtacc tcgtcgatgt cgaggccgat cagcgcggcc
     2881 aactcacgga tgtagccgct gaccatcgac tggtcccgca ccgacgccac cagcggtgcc
     2941 gccgcgcgca gcgcctgcac ccgcccctcg acacggtcca ggtcgtagcg ccccacgatg
     3001 ttgcccatca cgaaccggta cagcggctcc cgtcggccga ccagctcacg gaccgccgcc
     3061 tcaccttgct gcaaccgcag gtcgcacgga tcgagcccgg tgggctccac cgcgacgtag
     3121 gtctgcgaga cgaagttcgc gtcctcggcg aacaccttga gcgcggcgtt ctggcccgcc
     3181 tcgtccccgt cgaaggtgaa gatcacctcg ccgtggaagg cgtcgtggtc acccatcaac
     3241 cgccgcagca gccgggcgtg ctcgtgaccg aaggcggtgc cacaggacgc gaccgcggtg
     3301 tccacaccgg caaggtggca ggccatcacg tcggtgtagc cctccacgat caccgcctgc
     3361 gacttcttgc cgaccgcggt gcgggcgagg tcgagcccgt acagcacctg ggacttcttg
     3421 tagagcgggg tttccggagt gttgaggtac ttcgcgggca tccggtcgtc gtcatgcagc
     3481 cggcgcgccc cgaagccgag caccgaggtg gccgagtccc ggatcggcca cagcaaccgt
     3541 ccctggaaga agtcccaacc ccgttcgcgg accagcccgc cggcgaccaa ctcctggtga
     3601 ctgaacccct tggcacgcag gtggtcggcg agctcgcggc cgccgcgtgg tgcgtagccg
     3661 aggccgaagc gttcggcggc gtcgcggtcg aacccgcgtt cggcgaggaa ctggcgggcc
     3721 accagcgcgt cgggaccggc gaggtgctcg gcgaagaact ccgcggccaa ctggtgcgcc
     3781 tcgagcagcc gcatccgcag cccgggggcg accgctgggc cgcccccctc ggtgaagcgc
     3841 aactgcaccc cgacccggtc ggccagccgc tgcacggcct cggtgaaggt gaggttgtcg
     3901 atcttctgca cgaaggtgat gacgtcgccg ccctcgccac agccgaagca gtagaacagc
     3961 cccttggccg gcgtcacctg gaaactcggg gtcttctcgt cgtggaacgg gcacagcccc
     4021 ttcttcgacc cgcccccggc gttgcgcaac tgcaggtgac cgccgatgac ctcgtcgatg
     4081 cgggcacgtt cccggacggc cgccacgtcc tcgtcgttga tcctgcctgc cacagccgca
     4141 gtgtacgtgg gcgcccacgg cgctgccggc gccccggtca tcgcccgtgc actggtcaga
     4201 gcaggaagct ccacaccagc acgacgacac acagcagcag catgccggtc gcgttgacga
     4261 acagcacccg gccgaagtcg cgcacccgta cgtgcgcacc gatcgcgaac aggaagtaga
     4321 ccaccaggca actggtggtc agcgggccca gccacgggac cacgaggccg gcc
//
LOCUS       DATGUK010000031         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_733177,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000031 DATGUK010000000
VERSION     DATGUK010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733177"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="VK103_00505"
     CDS             <1..1493
                     /locus_tag="VK103_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HLQ81986.1"
                     /translation="IWLTWELTERWDVLAGAWRTGDDDRAGQVLQRDLAALAGWPHFR
                     NWRDEVGLPTGHLAMGLARGLALPGWDARWRAEAERAGRQLLDADVGDWFDRTWLRDA
                     PLHNIPIIVLCSVAALAAQLDHHDAGRYRARAVTAVEDWFTARKRDRHTEQTAYNGYL
                     FDAVTQWMELDRAAGGDLVERWQDPLTALCPEWVHSTVPGRADLAVPLGDVESEMTMW
                     LSCVTRLCEWYGVAATSGAGWLLRRCAPPRLPADALLRCLAGAVDADPGPPATGPQEL
                     PTAVAVRDGWELDDPAVVVGLARVPVGHLHHDAGQVVLAWRGACWVTDPGYQQYRRGP
                     EREYSIGPAAHNAPVVDGQAQTQRDCQLLGTDPVVVDLSPAYAGLPDGAVVRRTVALT
                     DDRLVVDDEVTGAGQQVITHWLAGARLAWAFVDGWVRWTDGTAALWCGTGTGPDDADR
                     DLLDPDGVERHEGSRGPVGLRHAGTAGRRRWQFVWDRSGGWQPPRW"
     gene            complement(1526..>2103)
                     /locus_tag="VK103_00510"
     CDS             complement(1526..>2103)
                     /locus_tag="VK103_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HLQ81987.1"
                     /translation="APVLVAMLGGSSMAADVACDNSAFALHLLSGVSGRDDRWGRALT
                     YLWILVPMVLVMTLAAAAVSRDWTHVPASLAISAVLVLAGIGAGAVVGVHLPGKVAEP
                     GASAFAASSSGNLQSMLGMGLVWLLTGMVSLPTLALVIGSLVGPVWLGWIALPVGVGT
                     GLAALTIGVRTGGRRLDHRAPELLATVSTVG"
BASE COUNT          285 a          762 c          797 g          259 t
ORIGIN      
        1 tgatctggtt gacctgggag ctcaccgaac ggtgggacgt gctcgccggg gcgtggcgca
       61 ccggcgacga cgaccgggcc gggcaggtgc tgcagcgtga cctcgccgcg cttgccgggt
      121 ggccgcactt ccgcaactgg cgcgacgagg tcgggctgcc gaccgggcac ctggcgatgg
      181 ggctggcccg cggcctggcc ctgcccggct gggatgcccg ctggcgggcg gaggcggaac
      241 gggcgggtcg acagctgctg gacgccgacg tcggtgactg gttcgaccgg acctggctgc
      301 gggacgcccc gctgcacaac atcccgatca tcgtgttgtg ctcggtcgcc gcccttgccg
      361 cccagctgga ccaccacgac gccgggcggt accgcgcacg tgccgtcacc gcagtcgagg
      421 actggttcac cgcccggaag cgcgaccgtc acaccgaaca gaccgcgtac aacggctacc
      481 tgttcgacgc cgtcacccag tggatggagc tggaccgtgc cgccggcggc gacctggtcg
      541 agcgctggca ggatccgctg accgcgctct gcccggaatg ggtgcactcc acggtgccgg
      601 gccgggccga cctggccgtc ccgctggggg acgtggagtc cgagatgacg atgtggctga
      661 gctgcgtcac ccggctgtgc gagtggtacg gcgtcgccgc gacctcgggc gccgggtggt
      721 tgctgcgtcg gtgcgcaccc ccacggctcc cggccgatgc cctgctgcgg tgcctggccg
      781 gtgcggtcga cgccgacccc ggtccgccgg cgaccggtcc gcaggaactg ccgaccgcgg
      841 tcgcggtgcg ggacggctgg gagctcgacg atccggcggt ggtggtcggt ctggcccgcg
      901 tcccggtcgg acacctgcac cacgacgccg ggcaggtggt gctggcgtgg cggggtgcct
      961 gttgggtgac cgacccgggc taccagcagt accggcgggg cccggaacgc gagtacagca
     1021 tcggcccggc cgcccacaac gccccggtgg tcgacgggca ggcccagacg cagcgggact
     1081 gccagctgct cgggaccgat ccggtggtgg tcgatctctc ccccgcctac gccgggctgc
     1141 ccgacggtgc cgtggtgcgc cggacggtgg cgctcaccga cgaccggctg gtcgtggacg
     1201 acgaggtcac cggggccggg cagcaggtca tcacccactg gctggccggg gcccggttgg
     1261 cgtgggcgtt cgtggacggc tgggtgcggt ggaccgacgg caccgctgcg ctgtggtgcg
     1321 ggaccggcac cggccccgac gacgccgacc gcgacctgct ggacccggac ggggtcgagc
     1381 ggcacgaggg cagccgaggg ccggtggggc tgcggcacgc cgggacggcg ggccggcgtc
     1441 gttggcagtt cgtgtgggac cgctcgggcg gatggcagcc accgcgctgg tgagaccggc
     1501 cgccggcatc ggcgacgggt ccggctcagc cgacggtgct gacggtggcg agcagttcgg
     1561 gtgcccggtg gtccaggcgc cgtccgcccg tacggacccc gatcgtgagt gccgcgagtc
     1621 ccgtaccgac cccgaccggc agcgcgatcc aacccaacca caccggaccg accagggacc
     1681 cgatcaccaa cgcgagggtg ggcaaggaga ccatgccggt gagcagccac accaggccca
     1741 tcccgagcat cgactgcaga ttgccgctgc tgctcgcggc aaaggcactg gccccgggct
     1801 cggcgacctt gcccggcagg tggaccccga ccaccgcacc ggcaccgatg ccggcgagca
     1861 cgagcacggc actgatcgcc aacgacgcgg ggacatgggt ccagtcgcgg ctcaccgcgg
     1921 ccgcggccag cgtcatcacc agcaccatcg gcaccaggat ccacaggtac gtcagcgccc
     1981 ggccccaccg gtcgtcacgg cccgagaccc cgctcaacag gtgcagggcg aaggccgagt
     2041 tgtcgcacgc gacgtcggcg gccatcgagc tgccccccag catcgccacc agcaccggcg
     2101 ccc
//
LOCUS       DATGUK010000032         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_35185,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000032 DATGUK010000000
VERSION     DATGUK010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35185"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VK103_00515"
     CDS             complement(<1..658)
                     /locus_tag="VK103_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HLQ81988.1"
                     /translation="MSAGTPADPAGPAALDGTDVADGTEPPAPWQRLERRTVAVTAIL
                     LTGAALLAAIPTSIAVAGATSPYTALAWVVPAAVLLIAGGGFADYLRWRKTSYRITPT
                     HVEVHKGILFTSRLRLARDRIRTVDVTAHPVLRWFALVRLAIGTGEQGGGGAAASQTI
                     VLNPVTRRSGEHLANELLDRLPADAATSGSERLATWRAAWTRYAPLSVLTPILVGVVI
                     G"
     gene            complement(655..1173)
                     /locus_tag="VK103_00520"
     CDS             complement(655..1173)
                     /locus_tag="VK103_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HLQ81989.1"
                     /translation="MKTPATSRPPGTIELRPPTNGYDRRVVRWWRSQLLVATGMLSLP
                     LVILGVLIAAARTWLLVPAAVIVVVGLLTALTLPLWWYTFHRWEVTQRAVYTRTGYFW
                     QTWRMAPMSRIQTVDTTRGPLQRLFGIATVTVTTASSAGAVDIAGLDFARAEEVAHEL
                     TLITDATPGDAT"
     gene            1325..1921
                     /locus_tag="VK103_00525"
     CDS             1325..1921
                     /locus_tag="VK103_00525"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLQ81990.1"
                     /translation="MPKVVDHDHRRTQIAEAVLSVVAGGGVPAATLRRVAAESGYPMS
                     TIQHYFRSTQQMLQYALALMEARKAEQINARVRSEWAHGPRAVLEGIFDEVLPLTPER
                     VRDTAIGAAYYVAWQHDEELKTLLLQDIPAARAGFAAVIVEGQRRGQIDPGREADREA
                     ELLFTLADALATGIIVGYQRPEEAQRTADYYLDRLFTP"
BASE COUNT          330 a          698 c          746 g          295 t
ORIGIN      
        1 cgccgatcac gacgccgacc aggatcggcg tgagcaccga cagcggcgcg tagcgcgtcc
       61 aggcggcgcg ccaggtggcg agccgctcac tgccggaggt cgccgcgtca gccggaagcc
      121 ggtccagcag ctcattggcc aggtgctccc cggaccgccg ggtgaccggg ttcagcacga
      181 tggtctgact cgccgctgcg cccccgccgc cctgctcccc ggtgccgatg gctaaacgca
      241 ccagggcgaa ccagcgcagc accgggtggg cggtgacgtc caccgtccgg atccggtccc
      301 gggccagccg cagccgactg gtgaacagga tgcccttgtg cacctccacg tgggtggggg
      361 tgatccggta gctcgtcttg cgccaccgca ggtagtcggc gaaccccccg cctgcgatca
      421 gcagcaccgc tgcgggcacc acccaggcca gggcggtgta cggcgaggtc gccccggcca
      481 ctgcgatcga ggtcgggatc gcggccagca gcgctgcgcc ggtgagcagg atcgcggtga
      541 cggccactgt gcgccgctcc agacgctgcc agggcgccgg cggctcggtg ccatcggcga
      601 catcggtgcc atcgagggcg gcgggcccgg ccgggtctgc gggtgtgccg gcgctcatgt
      661 ggcgtccccg ggagtggcat cggtgatcag ggtcagttcg tgggccacct cctcggcccg
      721 ggcgaagtcc agaccagcga tgtcgaccgc accggcggag gaggcggtgg tcacggtgac
      781 cgtggcgatc ccgaagagtc gctgcaacgg cccgcgggta gtgtcgacgg tctggatacg
      841 cgacatcggg gccatccgcc aggtctgcca gaagtagccg gtgcgggtat agaccgcccg
      901 ctgggtcacc tcccagcggt ggaaggtgta ccaccagagc ggcagggtga gtgcggtcag
      961 aaggccgacg acgacgatca ccgcggccgg taccagcaac caggtgcggg ccgcggcgat
     1021 cagcaccccg aggatgacca acgggagcga gagcataccg gtggccacca gtagctgact
     1081 gcgccaccag cgcaccaccc ggcggtcata gccattggtc ggtggccgca gctcgatggt
     1141 tcccggcggc ctgctcgtgg ccggtgtctt catggcgcgg cgtcctgcgc ggcgcagttc
     1201 ttcgccacgg cacgttgccc ctcgaacccg cggttcggcg ttagtgacac gacttcccct
     1261 tacgttatcg tgcaagtgca cgaaaaccta ccattgggtg gtcgcaggcg gtagaggagg
     1321 acggatgccc aaggtggtgg accacgatca ccgccgcacc cagatcgctg aggccgtgtt
     1381 gtcggtcgtg gccggcggcg gggtcccggc ggcgaccttg cgccgggtgg cggcggagtc
     1441 cggatacccg atgagcacga tccagcacta cttccgcagc acccagcaga tgttgcaata
     1501 cgcgttggct ctaatggagg cccgtaaggc cgagcagatc aacgcgcggg tgcgcagcga
     1561 gtgggcccac ggaccacgcg cggtgctcga gggaatcttc gatgaggtcc tgccgctgac
     1621 ccccgaacgg gtacgcgata ccgccatcgg agcggcctac tacgtcgcct ggcagcacga
     1681 tgaggaactc aagacactgc tgctgcagga catcccagcc gcacgcgcag gttttgccgc
     1741 cgtcatcgtc gagggtcagc gccgcgggca gatcgatccc ggccgagagg cggaccggga
     1801 ggccgagttg ttgttcaccc tggccgacgc actcgccacc ggcatcattg tgggctatca
     1861 gcgacccgag gaggctcagc gcaccgcaga ctactacctg gaccggctgt tcacgccatg
     1921 aatccggcgc aggtgcgcac cgcccgcacc tggaggcagc acctggtcca gggtggtcaa
     1981 ggtcccgggt gtgtgcaccc ccgccggggc ggggcccagg aggcagtggg ctatcgctgc
     2041 ggcggtgatc gccgtgaggc cgtaatcgg
//
LOCUS       DATGUK010000033         3806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1665073,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000033 DATGUK010000000
VERSION     DATGUK010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665073"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..2023
                     /locus_tag="VK103_00530"
     CDS             167..2023
                     /locus_tag="VK103_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81991.1"
                     /translation="MTTRLRGPMRTVLTHRATFGTGPDGAPLAYAVSQGTDSPLSVVD
                     LAGRRLAGPSLPGSTGAWGLVGGPDGQCWIGSYYDGRSYHWDGHRVHDLGRPTPETRY
                     TWDLALTEDGTVLFGTYPDAALVAHHPERGFVLLRRFGTDEVQYLRSLCWDPQRRIVW
                     CATGVTPNQVRGVFVDEPDREPLIVAGLDPTAVAHQLTLLGDRLFVVAGRRVWVADPD
                     TLRARLLIDGSGGRLSEEYRGGSYFTGVDDRLSRLDLASLRITEVPTDGDATGPYGGL
                     LGSTVHHDTLHLIAGEQTPELVQVRDGAVRSRLPADVVATPPHMLHVLGLADGPELLV
                     SAGQQGQIARWNPDTDLLGAPVSVGQVESWCWYDGEVYCGGYPRASLTVWDPDCVTPP
                     RELVSLHDSHHQSRPVTVAVGDGAAHLGTTPGYGLRDGALTTVDLDSGAATVRMFPDE
                     TVSAVAVDGDALLVGTSPEAGTGTPEHDGHGRLLRIAERGSGADTAVVPAPHGARAVT
                     ALTRIGDQWWLLADQWLCRFDPRTLEVTTAHRLADGPSGRGQLLVSGASLLTCTAGTV
                     RLVDPATGAARVLAEGVVRMALTDRALWCVVRPESGGSAATDFLRIDRADLA"
     gene            2070..2912
                     /locus_tag="VK103_00535"
     CDS             2070..2912
                     /locus_tag="VK103_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81992.1"
                     /translation="MPPDPVTTPPGPGRRRYSPFRSVIIVLCVIVALMVLVVGGLVAW
                     TQLRPVNSSDYSKVGNDASALKDELTPIVTRQSQMMDLSLLPTPEQVDRAAQAQRDQV
                     ATFRSDVVELGKLHGMGDPELQQLQEQLLATTDDQLTPMVEDFVTHYAAVLRMHQVCT
                     EYDKNAPTRPVDGADRAWFDDKIEQCEKATEEAAKSTVFQPFVEVRQQHITDSRAGYQ
                     AYGEATTDEARLAAQSTIGRAQNGIQTRGKEGLQQVVEANNAVFAEVEDGITAITAYT
                     DQHQ"
     gene            2998..>3806
                     /locus_tag="VK103_00540"
     CDS             2998..>3806
                     /locus_tag="VK103_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HLQ81993.1"
                     /translation="MLRSSNPVLSRRDAFTPGQSGHQFDDGMSGYRTTQQPGPIPPSG
                     GTGRMTIDDVLTKTAVLLGVLVLTAGAAWMLVPPAFVYPALIGSGLIGFVLSFVVAIR
                     RRINPVLAGIFAVVEGVFVGMLSMVFEQFYTGIVVTAVFSTMVAAGVTLAAYKFLNVR
                     VRGLVAKVAMVGTIAFAFAMLANLGLSFVGVDLGLRAGVTGPVGWLPILVSGIAVVLC
                     VLNLFLDFQYIENGIRMGAPANESWRAAFGLMVTLVWLYVELLRILSYFRRG"
BASE COUNT          536 a         1350 c         1355 g          565 t
ORIGIN      
        1 cttccgccac cggaccgcgc acatcctcga tacgctgacg ctcaggacgc gacgccgaac
       61 gccgtgttcc ccgcagaacc cggcgaatca aggtcatcaa cggcttgcgc gggtttgaga
      121 accacccagc gaggtgcacc aggccggccc gacacaggag acaacgatga ccacccggct
      181 ccgcggcccg atgcggacag tcctcaccca ccgtgccacc ttcggtaccg gccccgacgg
      241 cgcaccgctg gcgtacgcgg tcagccaggg caccgactcc ccactcagcg tcgtcgacct
      301 cgccggccgc cgcctcgccg gcccgtccct gccgggcagc accggcgcgt ggggactggt
      361 cggcggtccg gacggccagt gctggatcgg ctcgtactac gacggccgca gctaccactg
      421 ggacggccac cgggtccatg acctgggccg gccgacaccc gagacgcgct acacctggga
      481 cctcgcgctg accgaggacg gcaccgtgct gttcggcacc tatcccgacg ccgcgttggt
      541 ggcacaccac ccggagcgcg gcttcgtcct gctgcgccgg ttcggtaccg acgaggtgca
      601 gtacctgcgg tcgctgtgct gggacccgca gcgccggatc gtgtggtgcg cgaccggtgt
      661 cacgccgaac caggtccgcg gggtgttcgt cgacgagccc gaccgggagc cgctcatcgt
      721 cgccgggctc gacccgaccg cggtggccca ccagctgacc ctgttggggg accgactgtt
      781 cgtggtggcg ggccgacggg tctgggtcgc cgatccggac accctgcggg cgcggctgct
      841 gatcgacggc tccggtgggc ggctgtcgga ggagtaccgg ggcggcagct acttcaccgg
      901 ggtcgacgac cggctgagca ggctggacct ggcgagtctg cggatcaccg aggtgccgac
      961 cgacggggac gccaccgggc cgtacggagg cctcctcggc tccacggtcc accacgacac
     1021 gctgcacctc atcgcggggg agcagacacc ggaactggtg caggtccgtg acggcgcggt
     1081 ccgctcgcgg ctgcccgccg acgtggtcgc cactccgccg cacatgctgc acgtgctcgg
     1141 cctcgccgac ggcccggagc tgctggtgtc agccggccag cagggccaga tcgcacgctg
     1201 gaaccccgac accgacctgc tgggggcgcc ggtcagcgtc ggtcaggtcg agtcgtggtg
     1261 ctggtacgac ggtgaggtgt actgcggcgg ctatccgcgg gcctcgttga cagtgtggga
     1321 cccggactgt gtcactccgc cgcgggagct ggtcagcctg cacgactcgc accaccagtc
     1381 ccgcccggtc acggtcgcgg tgggcgacgg tgccgcgcac ctgggcacca cccccgggta
     1441 cgggttgcgg gacggtgcgc tcacgacggt cgacctcgac agtggtgcgg cgacggtccg
     1501 gatgttcccc gacgagaccg tcagcgcggt cgcggtggac ggtgacgcgc tgctggtcgg
     1561 gaccagtccg gaggccggga cggggacacc ggagcacgac gggcacggac ggctgctgcg
     1621 gatcgcggag cggggatccg gcgccgacac cgccgtggtg cccgcgccgc acggggcccg
     1681 tgcggtcacg gccctgaccc ggatcggtga ccagtggtgg ctgctcgccg accagtggct
     1741 gtgccggttc gacccacgga ccctggaggt caccaccgcc caccgactcg ccgacggccc
     1801 gagcggacgg ggacagctgc tggtcagcgg tgcctcgctg ctcacctgca ccgccggcac
     1861 ggtgcggttg gtcgacccgg cgaccggtgc ggcacgggtg ctggccgagg gggtggtccg
     1921 gatggcactc accgaccggg cgctgtggtg cgtggtccgg cccgagtccg gcggctcggc
     1981 ggcgaccgac ttcctccgga tcgaccgggc cgacctcgca tgagggcatc ggcgccccac
     2041 ggtctggcag actcggcacg gtgagcgcga tgccccctga ccccgtgacc acccctcccg
     2101 gccccggccg ccgccgctac tccccgttcc gctcggtcat catcgtgctg tgcgtgatcg
     2161 tggcgctgat ggtgctggtg gtcggcgggc tggtcgcctg gacacagctg cggcccgtca
     2221 actcgtccga ctacagcaag gtcggcaacg acgcgtccgc actgaaggac gagctcaccc
     2281 cgatcgtcac ccggcagtcg cagatgatgg acctgtcact gttgcccacc ccggagcagg
     2341 tcgaccgcgc ggcgcaggcg cagcgcgacc aggtggccac gttccgctcc gatgtcgtcg
     2401 agctcggcaa gctgcacggc atgggcgacc cggagctgca gcagctgcag gaacagctgc
     2461 tcgccaccac cgatgaccaa ctcactccga tggtggagga cttcgtgacc cactacgcgg
     2521 cggtgctgcg gatgcaccag gtgtgcaccg agtacgacaa gaacgcgccg acccggccgg
     2581 tcgacggtgc cgaccgggcc tggttcgacg acaagatcga gcagtgcgag aaggcgaccg
     2641 aggaagccgc gaagtcgacg gtgttccagc ccttcgtgga ggtccggcag cagcacatca
     2701 ccgactcccg ggcgggctac caggcgtatg gggaggccac caccgacgag gcccggctcg
     2761 ccgctcagtc gacgatcggc cgtgcgcaga acggcatcca gacccgcggc aaggagggcc
     2821 tgcagcaggt ggtcgaggcc aacaacgccg tgttcgccga ggtggaggac gggatcactg
     2881 cgatcaccgc ctacaccgac cagcaccagt gaggccgccg ggtcgcctgg tcgaccggcg
     2941 cggcgcgttg cgcagggccg atagactggg acgagcaaca ctcccaggag gtcattgatg
     3001 ctgcgcagct ccaacccggt gctttcccgc cgcgacgcgt tcacgcccgg tcagtcgggc
     3061 caccagttcg acgacggcat gtcgggctac cgcaccacgc agcagccggg gccgatcccg
     3121 ccctccggag gcaccggccg gatgacgatc gacgacgtgc tcaccaagac cgcggtgctg
     3181 ctcggggtgc tggtgctgac cgcgggcgcg gcgtggatgc tggtcccgcc ggccttcgtc
     3241 tacccggcgc tcatcggctc cggcctgatc ggcttcgtgc tgtcgttcgt cgtcgcgatc
     3301 cgtcggcgca tcaacccggt gctcgccggc atcttcgccg tcgtcgaagg cgtgttcgtc
     3361 ggcatgctgt cgatggtctt cgagcagttc tacaccggga tcgtggtgac cgcggtcttc
     3421 tccacgatgg tcgcggccgg ggtgacgttg gcggcgtaca agttcctcaa cgtgcgcgtc
     3481 cgtggcctgg tggccaaggt cgcgatggtc ggcaccatcg cgttcgcatt cgcgatgctg
     3541 gcgaacctcg gcctctcctt cgtcggtgtc gacctcgggc tgcgcgccgg ggtgaccgga
     3601 ccggtcggct ggctgccgat cctggtctcc ggcatcgcgg tggtgctgtg cgtgttgaac
     3661 ctgttcctcg acttccagta catcgagaac ggcatccgga tgggggcccc ggccaacgag
     3721 tcgtggcgcg ccgcattcgg gctgatggtg accctggtgt ggctctacgt ggagctgctg
     3781 cgcatcctgt cgtacttccg gcgcgg
//
LOCUS       DATGUK010000034         3288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1320480,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000034 DATGUK010000000
VERSION     DATGUK010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320480"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..768
                     /locus_tag="VK103_00545"
     CDS             79..768
                     /locus_tag="VK103_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ81994.1"
                     /translation="MHGRVDVVEMWMLVPIAVLVIGVAVVLYGALRDRTRRRQAEAML
                     GAPPDTQIPGFTPKRAPAYLVGPHAHERPTPLPELPEEPAARRDGTRIPYGFASPEFA
                     THVGPVAVLDDPRVLVCSDPVASVVEVLHALARHATDGRTAVLVAPEVPEQVCTTLQV
                     NVVQGKLAILVVPVADESARAELASLVGATPVDVFDLHAGYVPAEHLGHAGQWVSDAR
                     ASWVLQPQTAG"
     gene            874..1239
                     /locus_tag="VK103_00550"
     CDS             874..1239
                     /locus_tag="VK103_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HLQ81995.1"
                     /translation="MANKRWPQWVYGIGSEPDARFTLANERTFLAWIRTGLGFVTAAV
                     AIFALVSLRPELELEARIASLVLAVSGVACGVHAILGWARNERALRQSKPLPNSRLMP
                     ILVLVLVIVALIAAALVLA"
     gene            1236..1595
                     /locus_tag="VK103_00555"
     CDS             1236..1595
                     /locus_tag="VK103_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HLQ81996.1"
                     /translation="MSTDDRTDRPWEHGVWDTGLQPERTRLSWQRTALSSAACALIIA
                     RLVGEANPYVGITVAVAAILCGIMIALTARRRLWHSTVAAHRSEPLPDARGFVWMTAL
                     IMVAGAGAVTLAMLPVL"
     gene            complement(1714..2076)
                     /locus_tag="VK103_00560"
     CDS             complement(1714..2076)
                     /locus_tag="VK103_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HLQ81997.1"
                     /translation="MGPVAVEMSAEEFGHEVDRALDAVPEELARLVRNCVVLVEEEPP
                     PEEPNLFGLYEGIPLTERDSWYGATLPDRILIFRGPLQRHCRDIEELVDEIHITVVHE
                     IAHHFGIDDARLHQLGYG"
     gene            2398..>3288
                     /locus_tag="VK103_00565"
     CDS             2398..>3288
                     /locus_tag="VK103_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HLQ81998.1"
                     /translation="MNKHAATRMRRVVGVLASAGLMAGIAATAVSPTATADADRLTTW
                     TALNIRSGPGTDHAIVGGLYPGQLIEAAGPSRNGWTPVTFNGKKAWLSSRYVKVAGTG
                     KASSQVADSTTASTEPGRRTTTTALNVRTGPSTSYRILNVLARGTAVATTGAYKNGYV
                     EIRYDGRSAWVATQYLTGSTGSATSAGTSKLPKVVGTRVATTALMIRTNSTSNFQSLG
                     DVPKGTKLSITGVTRNGVAQIIHKGAVRWVNANYLSKSKPATGTDISGISGNGLSGLT
                     SRSKSLLVKVHDRYPQITWYN"
BASE COUNT          518 a         1193 c         1087 g          490 t
ORIGIN      
        1 agctagaggc ccgtggaatg accgggacag cgtagcgccg cagacccacc gaccgcgacc
       61 gccaccgccc ggtcgcgggt gcatggcagg gtggatgtcg tggagatgtg gatgctggtg
      121 ccgatcgccg tcctggtgat cggcgtcgcg gtcgtgctgt acggcgcact gcgcgaccgg
      181 acccgccggc gacaggcaga ggcgatgctg ggagcgccgc ccgacacgca gatccccggc
      241 ttcaccccga aacgggcccc ggcctacctg gtcgggccgc acgctcacga acggcccacg
      301 ccgttgccgg agctgcccga ggagccggcc gcccggcgcg acgggacccg gatcccgtac
      361 ggcttcgcct caccggagtt cgccacccac gtcggcccgg tggcggtgct cgacgacccc
      421 cgggtgctgg tctgcagcga tccggtggcc tcggtggtgg aggtgctgca cgcgctcgcc
      481 cggcatgcca ccgacggccg gaccgccgtc ctggtggccc cggaggtgcc cgaacaggtg
      541 tgcaccacgc tccaggtcaa tgtggtgcag ggcaaactcg cgatcctcgt ggtgccggtc
      601 gccgacgagt ccgcccgcgc cgagctggcc tcgctcgtcg gcgccacccc tgtcgacgtg
      661 ttcgacctgc acgcgggcta cgtccccgcc gagcacctcg gccacgccgg gcagtgggtc
      721 agtgacgccc gggcgagctg ggtgctgcag ccgcagaccg cgggatagct cgcctgccgc
      781 cccacggcac gacccgcacg cgccccgcac ctgggcacca cgcccgtcct gaccgccgtc
      841 accgccatcg acggcggcgc taggatcgtc gccgtggcga acaagcggtg gccccagtgg
      901 gtgtacggca tcggctccga acccgatgcc cgtttcaccc ttgcgaacga acgcacgttc
      961 ctggcgtgga tccggaccgg gctggggttc gtcaccgccg ccgtggcgat cttcgccctg
     1021 gtgtcgctgc ggcccgagct ggagctggag gcccggatcg cctccctggt gctcgcggtc
     1081 agtggtgtgg cctgcggggt gcacgcgatc ctcggttggg cccgcaacga acgtgccttg
     1141 cggcagtcca agccgctgcc gaactcacga ttgatgccga tcctggtgct ggtgctggtg
     1201 attgtcgccc tgatcgcggc cgcgctcgtg ctggcatgag caccgacgac cggaccgatc
     1261 gcccgtggga gcacggtgtc tgggacaccg gtctacagcc ggaacgcact cggctgtcgt
     1321 ggcaacgcac cgccctgtcg tccgcggcat gcgcgttgat catcgcccgg ttggtcggcg
     1381 aggccaaccc gtacgtcggc atcacggtcg ccgtggccgc gatcctgtgc gggatcatga
     1441 tcgccctcac cgcgcgacgt cggctctggc actcgacagt ggcggcgcac cgttccgagc
     1501 cgttgccgga cgcccgcggg ttcgtctgga tgaccgcgtt gatcatggtg gccggtgccg
     1561 gtgcagtgac gctcgccatg ttgcccgtgc tgtgatccca ccacgccgac agcggaccca
     1621 ccagcatcct ccacggcaga gtctcaaccg tcactcgacc ctttctcaca gccgggcggt
     1681 ccggcccttc gcggcggcgc agcggcccgt cgctcagccg tagcccaact ggtgcagccg
     1741 ggcgtcgtcg atgccgaagt ggtgcgcgat ctcatgcacc acggtgatgt ggatctcgtc
     1801 gaccagttcc tcgatgtccc ggcagtgtcg ctggagcggt ccgcgaaaga tcaggatgcg
     1861 gtccggcaac gtcgccccgt accaggaatc cctttctgtc aaagggatcc cttcgtacag
     1921 gccgaacagg ttcggctcct ccggaggggg ctcctcctcc accaggacca cgcagttgcg
     1981 caccagccga gccagctcct ccggtacggc gtcaagcgcg cgatcaacct cgtggccgaa
     2041 ctcctcggcc gacatctcca ccgccacggg acccatcgta cggaccccgg ctccatgaga
     2101 tcacagcacc gaaattactt tcgtggcatt tggcgtgtcg acttgccacc atgcccgaac
     2161 agcccctcga gaccttgccg ttaccaatgt ggccggttta aagtcctgac acgtctgggg
     2221 cagctgtgac tgacgtgacg ccggtgccga ggggaaagcg acatcgacga tccacacggc
     2281 gccccgggag gccgggacca tcccggccgg acaactggaa aggccgcgac cacgcacacg
     2341 gtcgcggcga ctctctcgcg acggtccggg aaggccgtcg ggaaggaatg tggtgccgtg
     2401 aacaagcatg ccgcgacacg gatgcggcgc gtggtgggag tgctggcctc ggccggattg
     2461 atggccggga tcgccgcgac cgcggtgagc cccacggcaa ccgctgacgc cgaccgcctg
     2521 acgacctgga ccgccctcaa catccgttcc gggccgggca ccgaccatgc catcgtggga
     2581 gggctctacc caggtcagtt gatcgaggcc gccggcccgt cccgcaacgg ctggacacca
     2641 gtgaccttca acggcaagaa ggcttggctg tccagccggt acgtcaaggt cgccggcacg
     2701 ggcaaggcgt cctcccaggt cgccgactcg accacggcgt cgaccgagcc gggccgccgt
     2761 accaccacga ccgcgctcaa cgtgcgcacc ggacccagca cctcgtaccg gatcctcaac
     2821 gtgctcgcgc gcggcaccgc ggtggccacc accggcgcct acaagaacgg ctacgtggag
     2881 atccgctacg acggacggtc ggcctgggtg gcgacgcagt acctcaccgg cagcaccgga
     2941 tcggcgacca gcgccgggac cagcaagctg cccaaggtcg tcgggacccg ggttgccacc
     3001 accgcattga tgatcaggac gaactcgacc tcgaacttcc agagcctggg cgacgtcccg
     3061 aagggcacca agctctcgat caccggcgtc acccgcaacg gtgtcgccca gatcatccac
     3121 aagggcgcag tccgttgggt gaacgcgaac tacctcagca agtccaagcc ggccaccggc
     3181 accgacatct ccgggatctc cggcaacggc ctgtcggggc tgacctcgcg ctccaagtcg
     3241 ttgctggtga aggtgcacga ccgctacccg cagatcacct ggtacaac
//
LOCUS       DATGUK010000035         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_854258,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000035 DATGUK010000000
VERSION     DATGUK010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854258"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VK103_00570"
     CDS             <1..360
                     /locus_tag="VK103_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLQ81999.1"
                     /translation="PERGADGSVAGRIVALTSDHTTHNLPYGASKGALDRIVGAAARE
                     LADRGVRANVINPGPVDTGWMDDDIRRAALGATPAGRLGTAEDVANLVRFLLSDAGAW
                     INGQLLSSDGGFGSRLG"
     gene            complement(367..1377)
                     /locus_tag="VK103_00575"
     CDS             complement(367..1377)
                     /locus_tag="VK103_00575"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLQ82000.1"
                     /translation="MFAWAVIVGGLVAVVGLVAMLVRGLGSLPVVIVLLVLTVLGMLY
                     LWRFALYPRVVVEDRLWVVNPMHTHIFDPAEVLDIRPGPDGLVVSHPEGTIEAWAVQK
                     STGALRKDRRTRADQVADALLAALHGATADPVTDPVTEPVTEPEAAVPTEEELAQQEL
                     AEDDGDDGDDGDDGDDDLIIRRAHRDDLETLVALEEEINTEALAHIFSNGEPFPTDAV
                     SDRWETALSDRAYKVRVAEIDGRPVGYLCYDAEQLHQIGLLSDHSGRGHGRALVEYAI
                     DDMVARGADTARLQVLEDNTDARDFYRHLGWLETGDRRPCPYPPHPTELTMAIELDGE
                     AS"
     gene            1867..>2052
                     /locus_tag="VK103_00580"
     CDS             1867..>2052
                     /locus_tag="VK103_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82001.1"
                     /translation="MVVILSVALVAGGWALVRARDRAVGAEPVRISAPSASISASESS
                     SVTPEEGTGTPTGPASST"
BASE COUNT          291 a          732 c          730 g          299 t
ORIGIN      
        1 ccggagcgcg gcgccgacgg ttcggtggcg ggacggatcg tcgcgctcac cagcgaccac
       61 accacgcaca acctgccgta cggggccagc aagggtgccc tggaccggat cgtcggtgcg
      121 gccgcccggg agctcgcgga ccgcggtgtc cgggccaacg tgatcaaccc gggcccggtc
      181 gacaccggat ggatggacga cgacatccgt cgggcggcgc tcggcgcgac cccggcgggc
      241 cgtctgggga cggccgagga cgtggcgaac ctggtccgct tcctgctgtc ggacgccggc
      301 gcgtggatca acggccagtt gctgtccagc gacggcggct tcggctcccg gctgggttga
      361 gccgcctcag ctcgcctcac cgtcgagctc gatcgccatc gtcagctcgg tcggatgggg
      421 cgggtacggg cagggccggc ggtcgccggt ctccaaccat cccaagtgtc ggtagaagtc
      481 ccgcgcatcg gtgttgtcct ccaacacctg cagtcgtgcg gtgtcagcgc cgcgagccac
      541 catgtcgtcg atcgcgtact cgacgagcgc gcgcccgtgt ccgcggccac tgtggtcgga
      601 cagcaggccg atctggtgca gctgctcggc gtcgtagcac aggtagccga ctggtcggcc
      661 gtcgatctcg gcgacccgga ccttgtaggc acggtcggac agggccgtct cccagcggtc
      721 gctgacggcg tcggtgggga acggttcgcc attggagaag atgtgtgcca aggcctcggt
      781 gttgatctcc tcctcgagcg cgaccagggt ctccaggtcg tcccggtggg cccgccggat
      841 gatcaggtcg tcatcaccat catcaccatc atcgccgtca tcgccgtcat cctcggcgag
      901 ttcctgctgg gcgagttcct cctcggtcgg gacggcggcc tcgggctcgg tgacgggctc
      961 ggtgacgggg tcggtgacgg ggtcggcggt ggccccgtgc agcgccgcca gcagcgcgtc
     1021 ggcgacctgg tctgcccggg tccgacggtc cttgcgcagc gccccggtcg acttctgcac
     1081 cgcccacgcc tcgatcgtgc cctccggatg gctgacgacc agaccgtcgg gaccggggcg
     1141 gatgtcgagc acctcggcgg ggtcgaagat gtgggtgtgc atcgggttga ccacccacag
     1201 ccggtcctcg acgaccaccc gcggatacag cgcgaaccgc cacaggtaga gcatccccag
     1261 cacggtcagc acgagtagca cgatgaccac tggcaggctg ccgagcccac ggaccagcat
     1321 ggcgaccaga ccgacgacgg cgaccagacc gccgacgatg acggcccagg cgaacacgcg
     1381 ggtccccact gcgcacctga acacctcgcg aaccacgcca tgaatctacc cggtcgccgt
     1441 ggtcgacgtt cagggtggac caacgagttg tccccaacgc gaccggaagc agcagccctg
     1501 tccacagatc cgcgatcggc ctgagccgtt ggcacatcgg atcagcatcc tcaggacatg
     1561 gcacgatccg accaggacct gagcgagcgg ctccggcacc gcctggtgga ggtggtgcgt
     1621 cccgtcgccg cgaccgcgcc gcgtccgggg cggccgtcca ccacggcgcg ggccgcgccg
     1681 cggcgtgccc gacctgcgac ggacggcgtg gaccagaccg ccctgcgtac gctgatccgg
     1741 ccggcggact ccgggtggac ggaggacccc gggccgcagg acctcgatga cggtgccgcg
     1801 ctggaaccgt ccggccgccg tccgccacgc agcccggtga cattcgatcg tcgacatgtg
     1861 gtcgtggtgg tggtgatcct ctcggtcgcc ctggttgccg gcgggtgggc cctggtgcgg
     1921 gcccgcgacc gggccgtcgg tgcggagccg gtccggatca gcgctccctc ggcgtcgatc
     1981 tccgcctccg agtcgtcgtc cgtgacgccc gaggagggta cgggcacgcc cacaggcccg
     2041 gcgtcctcca cg
//
LOCUS       DATGUK010000036         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1089723,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000036 DATGUK010000000
VERSION     DATGUK010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089723"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VK103_00585"
     CDS             <1..1455
                     /locus_tag="VK103_00585"
                     /inference="COORDINATES: protein motif:HMM:NF025382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4232 domain-containing protein"
                     /protein_id="HLQ82002.1"
                     /translation="VAAALLVVVVRPRCAPWLAAGVAGAAATVIELTQRRGGPGDWVP
                     RSGPLRVLFGSTFTAADLCWIVAGTAVAWLAVRLVALQGAAPGAPGADGADGADSADG
                     ADEEVDVREPTGEPGGERFPVLPTTVDRSFRGHRGPGRPRWQTGIAIATAGVLVMLLG
                     GLAGWAIEDSGRRIPGLVEWAPTVAGLAVLAVGLLDAWRGLSHDDRLPDARAVGLRTA
                     AGLVLATGALAVSVGIIGAARTTSLPVVVGVVSVLWLVSTVLLAAVAGWGDGVPQRGR
                     AAVGALTAAATLALALGASGAPTPTSDRPAPPSTATRSTPAGPSATPYPSVVMPSPDA
                     TRTPAPDAPAGVPLCRVEQLQAHTRGWDAAMGDTAVTVVITNTGGHDCAVQGLPTISL
                     LQGRSPVRLRIDHESIDHPGRSDRPRRALVRAGGEVRVALLWAGHRNRADERTTQALT
                     LHAPGVGSIPVALDQGPAPFDLMDGGTVSVSGWR"
     gene            1590..2483
                     /locus_tag="VK103_00590"
     CDS             1590..2483
                     /locus_tag="VK103_00590"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HLQ82003.1"
                     /translation="MFDEAQLYSPVTQTDDGTVTVHLGADHPGVNDPVYRERRNAIAA
                     VALAWSPGEPVPDIDYSEDEQQVWRRASTELHRLHQVHACTEFLHAKEAVGLPEDRVP
                     QLREVTDRLQPLTGFQYHPAPGLVPLRQFYGSLADGIFHSTQYVRHPSEPLYTPEPDV
                     IHEVLGHGNLLGSPRFAAVKRAAGEAANRVETDEALQLVADVFWFSIEFGVVWEGSEL
                     KAYGAGILSSYGEIEEFRSMQIEPLDLTRMGSIDYDITKYQPVLFAAASLAEIEDVVG
                     GFFAEVDDDTPARLGVVRQPA"
BASE COUNT          331 a          995 c         1021 g          401 t
ORIGIN      
        1 gttgccgcgg cgctgctggt ggtcgtggtc cggccacgct gcgcgccctg gttggcggcc
       61 ggggtggccg gggccgctgc gacggtgatc gagctcaccc agcgccgcgg tgggcccggc
      121 gactgggtgc cccggtccgg gccgttgcgc gtgttgttcg gcagcacctt caccgctgcc
      181 gacctgtgct ggatcgtggc cgggaccgcg gtcgcgtggt tggcggtccg gctggtggca
      241 ctgcagggcg cggcgcccgg tgcgcccggt gcggacggtg cggacggtgc ggacagtgcg
      301 gacggtgcgg acgaggaagt cgatgtccgc gaaccgaccg gtgaacccgg gggtgagcgg
      361 ttcccggtcc tcccgaccac cgtcgaccgg tcgttccgcg gacaccgcgg accgggccgt
      421 ccccgctggc agaccgggat cgccatcgcg accgccggtg tcctggtgat gctgctcggc
      481 ggcctcgccg gctgggccat cgaggacagt ggccggcgga tccccgggct ggtggagtgg
      541 gcaccgacgg tggccggtct ggcggtgctg gcagtcgggc tgctggacgc ttggcggggc
      601 ctgagccacg acgaccggct gccggatgcg cgagcggtcg gtctgcggac cgcggccggg
      661 ctggtgctcg cgaccggggc gctcgccgtg tcggtcggca tcatcggtgc ggcccgcacc
      721 accagtctcc cggtggtcgt cggggtcgtc tcggtgctct ggctggtgtc gacggtgctc
      781 ctcgcggcgg tcgccggttg gggcgacggc gtcccgcagc gtggccgggc cgccgtcggt
      841 gcgctgaccg cggcggccac cctcgcgctc gcgctcggcg cctccggtgc gccgaccccg
      901 acatccgacc ggccggcccc gccgagcacc gcgacccggt cgacccccgc cgggccgtcg
      961 gcgacgccgt acccgagtgt cgtgatgccg agcccggacg ccacccgcac cccggcgccg
     1021 gacgcgcccg ccggcgtccc gctctgccgg gtcgagcagt tgcaggccca cacccgcggc
     1081 tgggacgcgg cgatgggcga caccgccgtg accgtggtca tcaccaacac cggcggccac
     1141 gactgcgcgg tgcagggcct gccgaccatc tccttgctgc agggacgcag tccggtgcgg
     1201 ctgcggatcg accacgagtc gatcgatcac ccgggtcgct ccgaccggcc ccgtcgcgcg
     1261 ctggtccggg ccgggggaga ggtacgggtg gcgctgctct gggcgggcca ccgcaaccgg
     1321 gccgacgagc ggaccaccca ggcgctcacc ctgcacgccc ccggtgtcgg cagcatcccg
     1381 gtggcactcg accaggggcc ggcgccgttc gacctgatgg acggcgggac ggtgagcgtc
     1441 agcgggtggc gctgagcagt ctgcccagtt gtctcacggc cggattgtcc acactggtca
     1501 atctgtgcgg tcctttcccg gatggttgtc cagcgtgacg gccggtgttg tgctgggtgt
     1561 gtcgcccacc gaggacgccg ggaggaccca tgttcgacga agcacagctg tactcgcccg
     1621 tcacgcagac cgacgacggc accgtcacgg tgcacctggg tgccgaccac cccggggtca
     1681 acgacccggt ctaccgcgag cgccgcaacg cgatcgccgc ggtcgcgctg gcgtggtcgc
     1741 ccggcgagcc ggtacccgac atcgactact ccgaggacga gcagcaggtg tggcggcgtg
     1801 ccagcaccga gctgcaccgg ctccaccagg tccacgcctg caccgagttc ctgcacgcca
     1861 aggaggccgt cgggttgccg gaggaccggg tgccgcagct gcgggaggtc accgaccggc
     1921 tgcagccact caccggcttc cagtaccacc cggccccggg cctggtgccg ctgcggcagt
     1981 tctacggctc gctcgccgac ggcatcttcc actccaccca gtacgtccgg cacccgtccg
     2041 agccgctgta caccccggag cccgacgtga tccacgaggt gctcggccac ggcaacctgc
     2101 tcggctcgcc ccggttcgcc gcagtgaagc gggccgccgg cgaggccgcc aaccgggtcg
     2161 agaccgacga ggcgctgcag ttggtcgccg acgtgttctg gttcagcatc gagttcggcg
     2221 tggtgtggga ggggtcggag ctgaaggcgt acggcgccgg gatcctctcc tcgtacgggg
     2281 agatcgagga gttccgctcg atgcagatcg agccgttgga cctgacgcgg atgggcagca
     2341 tcgactacga catcacgaag taccagccgg tgttgttcgc cgctgcgtcc ctggccgaga
     2401 tcgaggacgt ggtcggcggc ttcttcgccg aggtcgacga cgacacgccc gcccggctcg
     2461 gcgtggtgcg ccagccggcc tgacggccgc ccgggtcatc cgaaggcgct cgccacccac
     2521 cccggctgtg gtcgctcgtc cgcgtcccgg ctgtgggcgc agcatggaca gcgcttcccc
     2581 aatcgcgcta gcgttcatgg tgtgcccagg cttgacgtca cccgttccat cgccgtcgcc
     2641 atcgcgccgg accgactgcc tgacgtgatc acgccgcagg tgcggtccgc actggccgaa
     2701 cgctacgact gcgtgtgggc caccgacgaa ctcggcccgg aggaggtc
//
LOCUS       DATGUK010000037         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1324584,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000037 DATGUK010000000
VERSION     DATGUK010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324584"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VK103_00595"
     CDS             <1..210
                     /locus_tag="VK103_00595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLQ82004.1"
                     /translation="RYAQIQGVWLSPELRGRGLAAPLMAEVVRQARRSTPNVSLYVND
                     YNTPALRTYERVGFQRVGQFATILY"
     gene            300..3212
                     /locus_tag="VK103_00600"
     CDS             300..3212
                     /locus_tag="VK103_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HLQ82005.1"
                     /translation="MLAVACLAAGALLLGHPGRTRPYRSLTRPPARISLDHWVFRPNP
                     VLRTLRRTTVSAVSRIIDLTPTTVPPARYRYRAAATSRLLPPDAVPDTRLGPDPTGTT
                     DFAAADGLGWIPTEPGGPFDGRTIAWRALTVKDAGVDLECAWDAEVFVDRFLLDPDVL
                     GQVRRVRVLVPVGDRLRQVGQLDLRDRSADEPTLELPGPIGLDVGHPVRSCVVRFETV
                     LTDLVLTGGVTVVGTAMPPVGIFPAPAGAQLTDDLVPVGAFAGAVVRPVGPEAVAVPV
                     AEHLVARVAEAVDQDWRIESPGAWGSSGQGPEGLGALVITCAPGRTGDPDLTSLVDAL
                     GVDPAPESFALQVSHDQVVVVGDRRGLRHGAECVVHQLIMDGGVRQGELVDRPAHEFR
                     GVHLPLPGPSQVDHFRRLIRWLVVPARFNTVIVEVAGGMEFRRHPEINRTWQDAHARA
                     LAGEMAMPGHFSMVAPGECLSQHDTAALVAELKALDLDVIPEVQSLSHVQYLTLAHPE
                     IAEVAGEVDNTAEVDLFAADQPAGKHPSCYCPSNPASYKLILDVLTEVLDVFQPERHV
                     HLGHDEVYELGVCPTCRHRDPAELFADDVNRLCAVVAERGLRPIIWADMLQPEARYRA
                     AAALDRLPEDLLMMDFIWYFHRERDTEPYLLDHGREVVIGNLYSSHFPRWEQRSTRPG
                     IVGGQVSTWVALDDEAMVREGKYYDIAHVGVMLWSARHHRHHRPGLARRIAPFLTDLR
                     SRLTGAPPASSADGVDLLGPPSEVGTGPDEHPTEPAPPLGRRHGDPLLAALAARLDGP
                     LRVVEPGGRTTGYPLTAPFVLPPSDTTTSAATGLDLELPQGTTEVHLVQAVTRPVPRA
                     AWGPLPEIGRWRALTADNTEVALHSLTVGGTVGHLHSRYAAPLPTSYYRHQGYTGIYR
                     SDPVMVGQLDDGRDLCLYRTVWQLPADGPSATRLALELDADIGCSLLVAAAVAHRG"
BASE COUNT          463 a         1288 c         1228 g          492 t
ORIGIN      
        1 cggtacgccc agatccaggg cgtctggctg tccccggaac tgcgggggcg agggctcgca
       61 gcgccactga tggccgaggt ggtccggcag gcgcgtcgtt cgaccccgaa cgtgtcgctg
      121 tacgtcaacg actacaacac cccggcactg cgcacctacg agcgggtcgg gttccagcgg
      181 gtcggccagt tcgcgaccat cctctactga ggggtcggtg ctgggcgagg tctgtgctgg
      241 gcgagatctg tgctgggtca cggccgtacg gtcccaccga gggttgggtc cgaggaccgg
      301 tgctcgccgt cgcctgtctg gcggccggtg cgctgctcct gggacacccg gggcgcaccc
      361 gcccgtaccg gtccctgacc cggccgccgg cccggatcag tctcgaccac tgggtcttcc
      421 ggccgaaccc ggtcctgcgg acgctccgga gaactacggt gagcgccgtg tccaggatca
      481 tcgatctgac cccgaccacg gtgcctccgg cccgctaccg ctaccgcgcg gcagccacct
      541 cacggctgct cccgcccgat gccgtgcccg acacccgact cgggccggac ccgaccggca
      601 ccacggactt cgccgccgct gacggactcg gctggatccc caccgagccg gggggaccgt
      661 tcgacgggcg cacgatcgcc tggcgggcgc tgaccgtcaa ggacgccggg gtcgacctgg
      721 agtgtgcgtg ggacgccgag gtgttcgtgg accggttcct gctcgatccg gatgtgctgg
      781 gccaggtccg gagagtgcgg gtgctggtgc cggtcggcga ccggctccgg caggtcgggc
      841 aactcgacct gcgcgaccgg tccgctgacg agcccaccct ggaactgccc ggaccgatcg
      901 gcctcgacgt cggccacccg gtgcgatcgt gcgtggtcag gttcgagacc gtgctcaccg
      961 acctggtgct gaccggtggg gtcacggtgg tgggtacggc gatgcccccg gtcgggatct
     1021 tcccggctcc tgccggggcg cagctgaccg acgacctcgt cccggtgggc gccttcgcag
     1081 gcgcggtggt ccgcccggtc gggcccgagg ccgtcgccgt accggtcgcc gagcacctgg
     1141 tggcgcgggt cgccgaggcc gtcgaccagg actggcggat cgagtcgccg ggggcgtggg
     1201 ggagcagcgg gcagggaccg gagggtctcg gggccctggt gatcacgtgc gccccgggcc
     1261 ggaccggcga ccccgacctc acctccctgg tcgatgcgct cggtgtcgac ccggcaccgg
     1321 agtcgttcgc gctgcaggtg tcgcacgacc aggtggtcgt ggtgggggac cgccgcggcc
     1381 tccgccacgg cgcggagtgc gtggtgcacc agttgatcat ggacggcggc gtccggcagg
     1441 gggagctggt cgaccgtccc gcccacgagt tccgcggcgt ccacctgccg ctgccgggac
     1501 cgtcacaggt cgaccacttc cggcgattga tccggtggct cgtggtgccg gcacggttca
     1561 acaccgtgat cgtcgaggtc gccggcggta tggagttccg tcgccatccc gagatcaacc
     1621 gcacctggca ggacgcccat gcccgtgccc tggccgggga gatggcgatg cccggccact
     1681 tctcgatggt ggcgcccggc gagtgcctgt cccagcatga caccgcggcg ctggtcgccg
     1741 agctgaaggc gctcgacctg gacgtcatcc ccgaggtgca gtcgctgtcg cacgtgcagt
     1801 acctgacgct cgcccacccg gagatcgccg aggtcgccgg ggaggtggac aacaccgccg
     1861 aggtcgacct gttcgccgcc gaccagccgg ccgggaagca tcccagctgc tactgcccgt
     1921 cgaacccggc gtcgtacaag ttgatcctgg acgtgctcac cgaggtcctc gacgtgttcc
     1981 agccggaacg gcacgtccac ctcggccacg acgaggtcta cgagctcggt gtgtgcccga
     2041 cctgccggca ccgggacccg gccgagctgt tcgccgacga cgtcaaccgg ctgtgcgcgg
     2101 tcgtcgcgga acgcggcctg cgcccgatca tctgggccga catgctgcag ccggaggcgc
     2161 gttaccgggc cgcggctgcg ctcgaccggc tgcccgagga cctgctgatg atggacttca
     2221 tctggtactt ccaccgcgaa cgcgacaccg agccgtacct gctcgaccat ggtcgcgagg
     2281 tcgtcatcgg caacctctac tccagccact tcccccggtg ggagcagcgc tccacccgcc
     2341 cgggcatcgt cggcgggcag gtgtcgacct gggtcgccct cgacgacgag gcgatggtgc
     2401 gggagggcaa gtactacgac atcgcccacg tcggggtgat gctgtggagc gcgcgccacc
     2461 atcgccacca ccgcccggga ctggcccgcc ggatcgcccc gttcctcacc gacctgcgca
     2521 gtcgcctcac cggcgcacca ccggcgagca gcgccgacgg tgtcgacctg ctcggcccgc
     2581 cgagcgaggt agggacgggc cccgacgagc accccaccga gccggcccca cccctcggac
     2641 ggcgccacgg cgaccccctg ctcgccgccc tggcggcacg gctcgacgga ccgctccggg
     2701 tggtcgagcc cggcgggcgg accaccgggt acccgctcac cgcgccgttc gtgctgcccc
     2761 ccagtgacac cacgacctcg gcggcgaccg ggctggacct ggagctgccc caagggacca
     2821 ccgaggtgca cctggtgcag gcagtgaccc ggccggtgcc ccgagccgcc tggggtccgc
     2881 tgcccgagat cggacgctgg cgggcgctca ccgccgacaa caccgaggtg gccctgcact
     2941 ccctcaccgt cggcggcacg gtcggccacc tgcacagtag gtacgccgca ccgctgccga
     3001 cctcgtacta ccgccaccag ggctacaccg gcatctatcg cagtgacccg gtcatggtcg
     3061 gccaactcga cgacggccgc gacctgtgcc tgtaccgcac cgtgtggcag ctgcccgccg
     3121 acggcccgtc ggcgacgcgg ctcgccctgg agctggacgc cgacatcggc tgctcactgc
     3181 tggtggcggc cgctgtcgcc caccgagggt gaggcgccgg gtccgtcggt gggcggggca
     3241 ctcccgtgct gtactggcgc tgaccgatcc tgcgccccac ccgcagcaca aggagaccgt
     3301 gatgcccgag cagcgacctg cgacgatcag cgtgaccggt gaggcgaagg cccaccgtgc
     3361 cgccgaacgt ggcacggtgc acctgaccat cggcttcgag ggccgtcggc gtgccgaggt
     3421 gctgccaccc accgaagctc tgcacgccga ggtcaccgcc gtcctggggt c
//
LOCUS       DATGUK010000038         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1091876,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000038 DATGUK010000000
VERSION     DATGUK010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091876"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2389)
                     /locus_tag="VK103_00605"
     CDS             complement(<1..2389)
                     /locus_tag="VK103_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82006.1"
                     /translation="MQQALSGTRYGRVRVCVLVPVFGDPDDPATRPLSAQVETQVSQL
                     VLANANARVELLRCLVARPDAAPRREVAREGWHNVLLSPEDSRGPGLGRTVLTGDDDP
                     LQVAGPAAAALAGLTGLWAEVPQAPLDDVNPPPGRTVRLARTFTRGLTAGEVEHRVRN
                     GVLTTADGLPAPRQQSATAVYAEDVPAATSAMATALWSKHRSVLRGPRASVQPDAATE
                     VGGGQAFRMFLGFLWNSIKGSPNRWYSGVVHKVSSATAKVTHEMVFGAAPSSYAVVVG
                     GVRADGNPAGWEDLARASGEMSEVLNRGQHQQEARADLSTVMKDYVAGALTLADGGDH
                     ANGLPPIQVGSDRAVLRDPSACAPGPQDVLTIPTPLVDHVGHDRLHPADILGTQLVRT
                     RLGEASRNQHLAVDADRADAEVQGWQQRFAHSYPVQVGTTLVNQIQATAGEVRGLLQH
                     IRSAADVPDLSGEIQAKQHRLALAMRILLGAFLVFLAADIVAVIKDWIRWDYALWIGL
                     GIFLLWAVITGSLFFTGQRALFGELHRRRVASGQAEANRENLRHASNDLRRLTELYGH
                     YLAWSRVAGAVVGKPFGDPVPAAPDPEPLGDGLPLATRFGVAEVQPDQVSRAVVTIRR
                     DLYRIGWLTLPWEQLLAEAGARIGPEGYEVGERPERLFGMAPGPQSLLSRWADLLEQQ
                     GTGRTSGDRIWQYVLSTFDGQQTELKQSLVTQVRGGAATGPQPLDDFLANLDLAAAAV
                     AGGQPAAQLGPESFDGTVLAVHARMGSRTAVTRNWLQSSSSGLARLTVLVQLSDGF"
BASE COUNT          332 a          907 c          835 g          329 t
ORIGIN      
        1 ggaacccgtc actcagctgc accagcaccg tcaggcgggc cagcccactg ctgctggact
       61 gcagccagtt gcgggtcact gcggtccgcg accccatccg ggcgtgcacc gccagcaccg
      121 ttccgtcgaa cgactccgga cccaactggg cggccggctg cccaccggca accgctgccg
      181 cggccaggtc caagttggcc aagaagtcat cgagcggctg cggcccggtc gccgcacccc
      241 cgcggacctg ggtgaccagt gactgcttca actcggtctg ctgaccgtcg aaggtcgaca
      301 gcacgtactg ccagatccgg tccccggagg tccgaccggt cccctgctgt tccaacaggt
      361 cggcccagcg gctgagcagc gactgcggtc cgggtgccat cccgaacagc cgctcgggcc
      421 gttccccgac ctcgtagccc tcgggcccga tccgggcacc cgcctcggcg agcaactgct
      481 cccacggcag cgtcaaccac ccgattcggt acaggtcacg ccggatcgtc accactgcac
      541 gcgagacctg gtcgggctgc acctcggcca ccccgaaccg agtcgccaac ggcagcccgt
      601 cgccgagcgg ctccgggtcg ggggcggccg gcaccggatc tccgaacggc ttgccgacca
      661 cggccccggc gacccggctc caggcgaggt agtgcccgta cagctcggtc agtcggcgca
      721 ggtcgttgct ggcgtgccgc aggttctccc ggttggcctc cgcctgtccg gaggcaaccc
      781 gtcgtcggtg caactcaccg aacagggctc gttgtccggt gaagaacagg gacccggtga
      841 tcaccgccca cagcaggaag atgcccaacc cgatccagag cgcgtagtcc caccggatcc
      901 agtccttgat caccgcgacg atgtcggcgg ccaggaagac caggaaggct ccgagcagga
      961 tgcgcatcgc caacgccaac cggtgctgct tggcctggat ctccccgctc aggtcgggga
     1021 catcggcggc cgaccggatg tgctgcagca gcccccggac ctccccggcc gtcgcctgga
     1081 tctggttgac caaggtggtg ccgacctgca ccgggtagga gtgggcgaac cgctgctgcc
     1141 agccctgcac ctcggcgtcg gcccggtcgg cgtcgacggc caggtgctgg ttgcgggagg
     1201 cctcccccag ccgggtccgc accagttggg tgccgaggat gtcggccggg tgcagtcggt
     1261 cgtggccgac gtggtccacc agcggggtgg ggatggtcag cacgtcctgc ggtcccgggg
     1321 cgcacgccga cgggtcccgc agcaccgccc ggtccgatcc gacctggatc ggcggcagcc
     1381 cgttggcgtg gtcgccgccg tcggccagtg tgagcgcccc ggcgacgtag tccttcatca
     1441 ccgtcgacag gtcggcgcgg gcctcctgct ggtgctggcc gcggttgagc acctccgaca
     1501 tctcgccgga ggcccgggcc aggtcctccc atccggccgg gttgccgtcg gcacggacac
     1561 cgccgaccac caccgcgtac gaggagggtg cggccccgaa caccatctcg tgggtgacct
     1621 tcgcggtcgc cgagctgacc ttgtgcacca ccccgctgta ccagcggttc ggcgagccct
     1681 tgatcgagtt ccagaggaac ccgaggaaca tccggaacgc ctgcccgccg cccacctcgg
     1741 tggcggcgtc gggctgcacg ctggcccgtg gcccccgcag cacgctgcga tgcttgctcc
     1801 acaacgcggt cgccatcgcg gaggtggcgg cggggacgtc ctcggcgtac accgcggtcg
     1861 cgctctgctg gcggggcgcc ggtagtccgt cggcggtggt gagcaccccg ttgcggaccc
     1921 ggtgctcgac ctcaccggcc gtgagcccgc gggtgaacgt ccgggccaac cggacggtac
     1981 ggcccggtgg tgggttcacg tcgtcgagcg gtgcctgcgg cacctcggcc cacagcccgg
     2041 tgagcccggc cagcgcggca gccgccggcc cagcgacctg cagcgggtcg tcgtccccgg
     2101 tgaggacggt ccgacccagg ccgggcccgc ggctgtcctc cgggctgagc agcacgttgt
     2161 gccagccctc ccgggcgacc tcccggcgcg gggcggcatc cgggcgggcg accaggcagc
     2221 ggagcagttc cacccgggcg ttcgcgttcg ccagcaccag ttgcgagacc tgggtctcca
     2281 cctgcgccga cagcggccgg gtcgcgggat cgtcggggtc gccgaacacc gggaccagca
     2341 cgcagacccg cacccgcccg taccgggtgc cggagagcgc ctgctgcact ccggagccgt
     2401 acg
//
LOCUS       DATGUK010000039         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1556450,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000039 DATGUK010000000
VERSION     DATGUK010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556450"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VK103_00610"
     CDS             complement(<1..642)
                     /locus_tag="VK103_00610"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HLQ82007.1"
                     /translation="MRLAPIPTTVVTVLAAGALIGAAAAVDAPQSVTQEGETSTVPVA
                     QRTLVCPSLGGSEVTSAMLGMLALGEGPGEASGQSVPDEAVVTPLAPPADLLEDGAAG
                     ESPTASPSPSPSPTESQEVLTTVDERQAVIQQRVEVDEPTSYAVRATGAMAPGFTAEQ
                     SVVADSSEQYGLAGTSCSQPAREHWFVGSTGEVGDRGRLVLSNPSEAPAVVDVN"
     gene            complement(639..>2759)
                     /locus_tag="VK103_00615"
     CDS             complement(639..>2759)
                     /locus_tag="VK103_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82008.1"
                     /translation="GSGQLAGGALLPAPTDVGALWRTYTEAWHGVGLGSPTAAPPYLG
                     VVALLGTLFTSPSVAVDVLLLGSVPLSALTATMLLRRVVVSRWVRVWAAAAYAVLPAI
                     SGAIAAGRLGTAVACVVTPLIVLAAHRSLGAPARADVPAQPGTFRTAWTAGLLIAVAA
                     AFVPLTWVLAVVLGVAAVAVYRTKAVVWRVLAALVVTPVVLLPWTFEVVRSPVKLLSE
                     AGIPGPGLSDSDLGPGMVALLHPGGPGVAPLGLAAGLVLAGFAGLLLATRRGPVVAAW
                     GVGLLGLAAGVVLIRVPITGPTLESPVAGWPGYATVVIAGALIVATALAADDRVTLAE
                     SRPRGLWRPLFALLGLAAMSMPVLGGLWWAVRGAEDPLERRDPAVLPIYVAEQATSPE
                     HIRTLVLDRDEEQRVSYALLREAGPRLGSAETGPPVGSDEPLAELVGELASGLGTASG
                     AEVAAFGARYIYLPAPFDVGLADALDAVPGIVRASPPEGAAMWRVEDGAARVRVLPPD
                     SPEVTGEPADQTDTGTGAGADADDGAEDDAFTAPWTVLPSGPVDVDTSVPEGDDGRVV
                     VLAERTDPGWSASLDGTELEPVTVGGWAQGFELPASAGNLVITHEGEARAWWLRAQAV
                     LVVIAVILAMPAMRRSGGVVDDVADLDPEDTAPVGPPRTPETAPGARARPADSGPRER
                     RRPVVGRRRPEPYRGKRARGKEDR"
BASE COUNT          391 a         1028 c         1004 g          336 t
ORIGIN      
        1 attgacgtcg accacggccg gcgcctcact cgggttggac agcaccagcc ggccccggtc
       61 acccacctcg ccggtgctgc cgacgaacca gtgctcccgg gccggctggg aacacgacgt
      121 cccggccagg ccgtactgct cactgctgtc ggcgacgacg ctctgctccg cggtgaagcc
      181 cggggccatc gcgccggtgg cccgcacggc gtaggacgtg ggctcgtcca cctcgacccg
      241 ctgctggatc accgcctggc gctcgtcgac cgtcgtcagc acttcctgcg actcggtagg
      301 gctggggcta gggctcgggc tggcggttgg gctctccccc gcggcgccgt cctccaggag
      361 gtccgcaggt ggggcgagcg gggtcacgac ggcctcgtcg ggcaccgact gtccggaggc
      421 ctcgccgggg ccctccccca gcgccagcat gcccagcatc gccgaggtca cctcggagcc
      481 gccgagcgac gggcacacca gggtgcgttg cgccaccgga acggtgctgg tctcgccctc
      541 ctgggtcacc gactgcgggg cgtcgacggc cgcggcagcc ccgatgagcg cgccggcggc
      601 cagcacggtg accacggttg tcggtatcgg cgcgagcctc atctgtcctc cttgccgcgt
      661 gcgcgcttac ctcggtacgg ctcggggcgc cgtcggccca cgaccgggcg tcgcctctct
      721 cgcggcccgg aatctgctgg ccgggctcgc gcaccagggg cggtttccgg ggtacgtggc
      781 gggcccacgg gggcggtgtc ctccgggtcg aggtcggcga cgtcgtcgac caccccaccg
      841 gagcggcgca tggccggcat cgccaagatc acggcgatga ccaccaggac cgcctgagcc
      901 cgcagccacc atgcccgggc ttcgccctcg tgggtgatga ccaggtttcc ggcagatgcg
      961 ggaagctcga agccttgagc ccagccaccg accgtgacgg gttcgagctc ggttccgtcg
     1021 aggctggccg accagccggg gtcggtacgt tcggccagca cgacgacacg tccgtcatcc
     1081 ccctcgggca cggaggtgtc cacatcgacc ggaccactgg gcaacaccgt ccagggggcg
     1141 gtgaacgcat cgtcctctgc tccgtcgtct gcgtcggcac cggcgccggt ccccgtgtcg
     1201 gtctggtcgg ccggttcacc ggtgacctcg ggtgagtcgg gcggcagcac gcgcacgcgc
     1261 gccgcaccgt cctcgacgcg ccacatggca gcaccctcgg gtgggctggc ccggacgatg
     1321 ccgggcacgg cgtccagcgc gtcggcgagg ccgacgtcga atggtgcggg caggtagatg
     1381 tagcgggccc cgaacgcggc cacctccgcc ccactggcgg tgcccagacc ggaggccagc
     1441 tctccgacca gttcggccag tggctcgtcc gagcccaccg gtggtccggt ctcggcggac
     1501 ccgagccgcg gtcccgcttc gcgcagcagt gcataggaga cgcgttgttc ctcgtctcgg
     1561 tcgagcacga gggtgcggat gtgctccggg ctggtggcct gttcggccac gtagatgggc
     1621 aggaccgccg ggtcccggcg ttctagcggg tcctccgcgc cgcgtacggc ccaccagagg
     1681 ccgccgagca ccggcatgct catcgccgcg agaccgagca gcgcgaacaa tggccgccac
     1741 agcccgcgtg ggcgggactc ggcgagggtc acgcgatcgt cggccgcgag cgccgtggcc
     1801 acgatcaggg ccccagcgat gacgacggtg gcatagcccg gccacccggc caccgggctc
     1861 tccagcgtgg ggccggtgat ggggacgcgg atgaggacga cgccggccgc aagcccgagc
     1921 agccccacac cccatgccgc gaccaccggg ccgcgccggg ttgccagcag cagcccggcg
     1981 aaaccggcca acaccagacc ggcggccagt ccgaggggtg caacgccggg cccgcccggg
     2041 tggagcagcg cgaccatgcc cgggccgagg tcggagtcgg acaggcccgg acccggtatc
     2101 ccggcctcgc tgagcagttt caccggtgag cgcacgacct cgaacgtcca cggcagcagc
     2161 acgacgggtg tgacgaccag tgccgccagc acccgccaca cgaccgcctt ggtgcggtac
     2221 acggcgacgg cggccacgcc gagcacgacc gccagcaccc acgtgagggg gacaaaggct
     2281 gcggccaccg cgatcaacag tccggcggtc caggcagtgc ggaatgtgcc cggctgggcg
     2341 ggcacgtcgg cacgagccgg tgccccgagt gaccggtgcg cggccagcac gatcaacggg
     2401 gtcacgacgc acgcgacggc tgtgcccagc cgtcccgcgg cgatcgcacc gctgatggcg
     2461 ggcaggaccg cgtaggctgc ggccgcccag acccgcaccc agcgggagac cacgacccgg
     2521 cgtagcagca tggtggcggt cagggcggac aacggcacac tgcccagcag cagcacgtcc
     2581 accgcgaccg aggggctggt gaacagcgtg cccagcagcg cgaccacgcc gaggtagggc
     2641 ggtgccgcgg tggggctgcc gagcccgaca ccgtgccagg cctcggtgta ggtgcgccac
     2701 agcgcgccga cgtcggtcgg cgcgggcagt agcgctcccc cggccaactg tcccgagcc
//
LOCUS       DATGUK010000040         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_749211,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000040 DATGUK010000000
VERSION     DATGUK010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749211"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VK103_00620"
     CDS             complement(<1..1073)
                     /locus_tag="VK103_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HLQ82009.1"
                     /translation="MTQPSTGRPTPGPEIPGASRQRLADFPFFAGGVDAASPATPTAP
                     RQPVGRRAAATSAPAAWRDGVTVDWPQVRALTRRVSEEIQTQHGQRAASLTGIDRQMM
                     AEPIIAKVVADHAERQTRDGNLWSQHDVRAHVKAITDSVFGLGRLQPLFEIPTAENVN
                     INGHDSVVVVHQDGSREELPPVADSNDELIDQLRHIGQNSSPKRPLDAAHPEMTVMVD
                     GKFRLHAILNETSPVPSVSIRQHHLVAVSLADLAARGLMPMPVAEFLDAMVKANRTIV
                     VGGEIGAGKTTLLRALLDAIPLNERYATLETDLELFAHRQPGRGNNLVLHARSGMGET
                     TQDGDQIGQISVADQIPWALRQDV"
     gene            complement(1077..1919)
                     /locus_tag="VK103_00625"
     CDS             complement(1077..1919)
                     /locus_tag="VK103_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82010.1"
                     /translation="MAVLAFCSAAGSPGVTTTAIGVTQHWPGSAVLMDADCHSAVPTG
                     LLGGQQSVSGGLLRVLEAAQFGRDVRNAVWDQSMPLPGDDPDGIRRLFLPGMPTLPGL
                     RSLEQAWGEVAAGLAQLGPAGVDVIVDLGRITNPAGVHPALIGAISELCLLVRPTLRD
                     VAAARWVAGRIREQCGTTGKARVVVMAAGPHHRGFDDAEVAEALGLPTRTRIPHDPAW
                     AAVWSDGMAPPRRLGRDRTETGDAAKGKGGRGRYRHVVAHLAMSLHDRARVRPEAGFL
                     EGSV"
     gene            complement(1919..2467)
                     /locus_tag="VK103_00630"
     CDS             complement(1919..2467)
                     /locus_tag="VK103_00630"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HLQ82011.1"
                     /translation="MIAIVLGALLAWFVVQAMQTQHHVIVLRQDVPRGSVITAEALGQ
                     TTVGTIQGVSVVPAEQIPQLVGKTARIDLAKGALMPADATTEEQAPAEGKSLVGLMLR
                     PGKVVSGAIVPGSAVRLIALPGDSASTSGEPKTFQGTVVSVASAADGVSTLLNVEVPS
                     GVAPQVQLLAAQDRIAVVQDAA"
     gene            complement(2602..>2969)
                     /locus_tag="VK103_00635"
     CDS             complement(2602..>2969)
                     /locus_tag="VK103_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82012.1"
                     /translation="HPRSRSEILHQQPAGVSNAELILTAVSAQHEAVRAHFTADAAPR
                     PESGLFDTSTYQRRKRRRRPAGSVRPQQYGARLHHTHIATIEQLVSDLPHVSRSAFIA
                     ACLELHVLGAIDPYDSNTP"
BASE COUNT          481 a         1062 c          970 g          456 t
ORIGIN      
        1 acatcctgac gcaaggccca cggaatctgg tcagccaccg agatctgccc gatctgatcg
       61 ccgtcctggg tggtctcgcc catgcctgac cgggcgtgca ggaccaggtt gttgccccga
      121 ccaggttgcc gatgcgcgaa caactccagg tcggtctcca aggtggcgta acgctcgttc
      181 agcgggatcg cgtcgagcag ggcgcgcagc aaggtcgtct tgcccgcccc gatctcgccg
      241 ccgaccacga tggtgcggtt ggccttgacc atggcgtcga ggaactcggc caccggcatc
      301 ggcatcaacc cgcgggccgc cagatcggcc aggctgaccg cgaccagatg atgctggcgg
      361 atcgacaccg agggcaccgg gctggtctcg ttgaggatgg cgtgcaaccg gaacttgccg
      421 tcgaccatca ccgtcatctc gggatgtgcg gcgtccaacg gccgcttcgg gctgctgttc
      481 tggccgatgt ggcgcagctg atcgatcaac tcgtcgttgg aatcggccac cggcggcagc
      541 tcctcacgac tgccgtcctg gtgcaccacc acaaccgagt cgtgaccgtt gatgttgacg
      601 ttctccgcgg tgggaatctc aaacagcggc tggagccgcc ccagaccgaa caccgagtcg
      661 gtgatggcct tcacatgcgc ccgcacgtcg tgctgactcc acagattgcc gtcacgggtc
      721 tgccgctcgg catggtcagc aacgaccttg gcgatgatcg gctcagccat catctgtcgg
      781 tcaatgccgg tcaagctggc tgcccgctgc ccgtgctggg tctggatctc ctcactcacc
      841 cggcgagtca gggcccgcac ctgcggccag tcgacagtga ccccgtcacg ccaggccgct
      901 ggcgctgacg ttgcggccgc gcggcgcccg accggctggc ggggcgcagt cggtgtggct
      961 ggactggccg cgtcgacgcc gccggcgaag aacgggaagt cggccagtcg ttgacgcgac
     1021 gcaccaggaa tctccggccc cggggtcggg cgcccggtcg agggctgggt catgggtcac
     1081 acgctccctt cgaggaaacc ggcctccgga cgaacccgtg cacggtcgtg caagctcatg
     1141 gccaaatggg cgaccacgtg gcggtagcgg ccccggccac ccttgccctt ggctgcgtct
     1201 ccggtctcgg tccggtcgcg gcccaaccgg cgtggcggag ccattccgtc ggaccacact
     1261 gcggcccagg ccgggtcatg cgggatccgg gtgcgcgtgg gcaacccgag cgcctcggcg
     1321 acttcggcgt catcgaaacc ccggtggtgt ggaccagcag ccatcaccac cacccgcgcc
     1381 tttccggtgg tgccgcactg ctcccggatc cggccggcga cccaccgggc cgcggcgaca
     1441 tctcgcaagg tcggccggac gagcaagcag agctcgctga tggcaccgat cagtgccgga
     1501 tgcacgcctg cgggattggt gatccgaccc aggtcgacga tcacgtcaac cccggccggg
     1561 ccgagctggg ccagcccggc cgccacctca ccccacgcct gctcgagcga tcgcaacccc
     1621 ggcagtgtcg gcatccccgg aaggaacagg cgccggatcc cgtccggatc gtccccgggc
     1681 agcggcatcg actgatccca cacagcattg cgaacatctc ggccgaactg agcagcctcc
     1741 agcacccgca gcagaccccc tgagacggac tgctggccac ccaacaggcc cgtcgggacc
     1801 gcactgtggc agtcggcatc catcagcacc gctgaccctg gccagtgctg agtgactccg
     1861 atcgcggtcg tggtgacccc cggcgagccg gccgcagaac agaacgcgag gacggccatc
     1921 aggctgcgtc ctgaaccaca gcgatccggt cctgggccgc cagcagctgc acctgcgggg
     1981 ccacaccgga cgggacctcc acgttgagca acgtcgacac cccgtcggcc gccgacgcga
     2041 ccgacaccac cgtgccctgg aacgtcttcg gctcaccgct ggtcgatgcg ctgtcccccg
     2101 gtagagcgat cagccgcacc gcgctgccag gaacgatggc acccgagacc accttgcccg
     2161 gtctcagcat cagaccgacc aagctcttgc cttctgctgg agcctgctcc tcggtggtcg
     2221 catcggccgg catcaacgca cccttggcca gatcgatgcg ggcggtcttg cccaccagct
     2281 gcgggatctg ctcagccggc accaccgaca cgccctggat cgtgccaacg gtcgtctgcc
     2341 ccagcgcctc agccgtgatc accgagccgc gaggaacgtc ctggcgcagc acgatcacat
     2401 ggtgctgagt ctgcatcgcc tgcaccacga accaggccag cagcgccccc agcacgatcg
     2461 caatcactcc agccagcagc caccgcggcc gtacgcgagc ccgcgtcacc actggctgcg
     2521 gatgccgtgg tccgctgact ggctcgttga ccaccggggt cgccgtaccg gtggcaccag
     2581 tcggagtggg catcgtgcct cctagggggt gttggagtcg tacgggtcga tcgcgccaag
     2641 gacgtggagc tcaaggcatg cagcgatgaa agcacttctc gacacatggg gaaggtcgga
     2701 cacgagctgc tcgatggtcg caatgtgggt gtgatgcaga cgcgcaccgt actgctgcgg
     2761 ccggacgctg cccgccggcc gacgtcggcg cttgcgccgc tggtaggtac tggtgtcgaa
     2821 caggcccgac tccggtcgcg gtgcagcgtc tgcggtgaag tgcgcccgca ccgcctcgtg
     2881 ttgggcgctg accgctgtca agatcaactc ggcattgctg acgccggccg gctgttggtg
     2941 caagatctcg ctacgggagc gcgggtgca
//
LOCUS       DATGUK010000041         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1211968,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000041 DATGUK010000000
VERSION     DATGUK010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211968"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..932)
                     /locus_tag="VK103_00640"
     CDS             complement(312..932)
                     /locus_tag="VK103_00640"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016934279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HLQ82013.1"
                     /translation="MTVGEPLDLRLRALELAPALLGAVLGYGPVSIMLTEVEAYEGVD
                     DPASHAWRGRTPRSGIMFGPPGHVYVYLSHGIHHAMNLVCGPDGTASAVLLRAGRVVA
                     GLPEARRRRSGVEDDRLARGPGNLTRALGISLADNGATLRVTVAGRTGTPRRVGEGDI
                     ATGPRVGVSRAADRPWRFRVRDDATVSAYRRSGRAPAARDTASPDQ"
     gene            complement(970..1191)
                     /locus_tag="VK103_00645"
     CDS             complement(970..1191)
                     /locus_tag="VK103_00645"
                     /inference="COORDINATES: protein motif:HMM:NF036450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HLQ82014.1"
                     /translation="MAQHARGPQSASGAPEDPAAEIKRRMKEALERKNQQHHDNPEGA
                     PLEGGEHMHGVEGHVDTQTYRRKAGGGGA"
     gene            complement(1314..1904)
                     /locus_tag="VK103_00650"
     CDS             complement(1314..1904)
                     /locus_tag="VK103_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HLQ82015.1"
                     /translation="MARTPADRRAVVGIAGRPGAGKSTLAVALADRLGPAAVVVGMDG
                     FHLADAVLDQLGLTDRKGVPATFDPDGYRVLLSRLRTEPGPVYAPGFERTLEQPVAQA
                     IIVHPQHRLVITEGNYLLLPEPAWTGAREQLDQVWWLEGDEALRIERLRQRHVRHGKS
                     PEAADDWMARVDGPNAEVAARTADRADLVVRLDGRV"
BASE COUNT          317 a          817 c          738 g          319 t
ORIGIN      
        1 aaccgtgcca tcgtgcgtgt gtgtcctcca tgcagcccga cgagcacccc agacccttcg
       61 agcggcatca ttgatccgtc atcagctcgg ccccgaagcg ccggttgcga tgagcctagg
      121 cccaccacgg atggcgaggc ccggcaacac ccccatgttg ccgggcccca cagtgcggca
      181 gacgatccgt caccctcctc cgacgacgga tcgatgacgc catgactccc ccaaactgtc
      241 gcggcgagcc gctgaagtca ggctaagtgg gacaaatgtc ccagtcaagc gtcccagaac
      301 aagaattgcc gctattggtc cggggaagcg gtgtcccgtg ccgccggcgc ccggccactg
      361 cgccgatagg ccgacaccgt ggcgtcatca cgcacccgga accgccaggg acggtccgcc
      421 gcgcgactga cgccgacccg cgggccggtc gcgatgtcgc cctcgcccac ccgtcgcggc
      481 gttcccgttc ggccggcgac cgtgacccgc agtgtggcgc cgttgtcagc caggctgatg
      541 ccgagggcac gggtcaggtt gcccggcccc ctggccagcc ggtcgtcctc gacgcccgag
      601 cggcgccgac gggcctccgg gagaccggcg accactcgcc cggcgcgcag cagcacggcc
      661 gacgccgtac cgtccggtcc acacaccagg ttcatcgcgt ggtggatgcc gtgggacagg
      721 tagacgtaca cgtgcccggg cggaccgaac atgatccccg accgcggcgt ccggccccgc
      781 caggcatggg acgccgggtc gtcgacgccc tcgtaggcct ccacctcggt gagcatgatc
      841 gacaccggtc cgtacccgag caccgcgccg agcagtgccg gcgccaactc gagggcacgc
      901 aggcggaggt cgagcggctc cccgaccgtc agggtgaccg tacggggagc cttgaccggc
      961 cgacccgggt caggcgccgc cgccaccggc cttgcggcgg taggtctggg tgtcgacgtg
     1021 gccctccacg ccgtgcatgt gctcaccacc ctcgagcggg gcaccctccg gattgtcgtg
     1081 gtgctgctgg ttcttgcgtt ccagcgcctc cttcatccgg cgcttgatct cggccgcggg
     1141 atcctccggc gcaccgctgg ctgactgcgg tcctcgtgcg tgctgagcca tccggcccac
     1201 ctcctgggtg ctgacgggct tctgctcccc attgtgccgg gtacggcgtc gggcgccagc
     1261 ccttttgccc ggcgggaccg tcgggatgcg catcgacgcc ctcccgactc cgctcagacc
     1321 cgcccgtcga gccgtacgac caggtcggcc cggtctgcgg tgcgggcggc cacctcggcg
     1381 ttcgggccgt cgacgcgggc catccagtcg tcggccgcct ccggcgactt gccgtgccgg
     1441 acgtgccgct gacgcaaccg ctcgatccgc agtgcctcat caccctccag ccaccacacc
     1501 tggtcgagct gttcgcgggc cccggtccag gccggctcgg gcagcagcag gtagttgccc
     1561 tcggtgatca ccagccggtg ctgcgggtgg acgatgattg cctgcgcgac cggctgttcc
     1621 agggtgcgct cgaacccggg ggcgtacacg ggtcccggtt cggtgcgcag ccgtgacaac
     1681 agcacccggt agccgtccgg gtcgaaggtc gccggcaccc ctttccggtc ggtcagcccc
     1741 agctggtcga gcacagcgtc ggcaaggtgg aaaccgtcca tcccgacgac gaccgcggcc
     1801 gggccgagcc ggtcggcgag cgcgaccgcc agggtcgact tcccggcacc cggtcgaccg
     1861 gcgatgccga cgacggcacg tcggtctgcc ggggtgcggg ccatcagctg ctgcagccgg
     1921 acaacaaggg catcgggatc ggcgagctgg gtcaccgcac catcctcggc gatggcggca
     1981 gggacgccaa cccacctgtc ctcggcactc cgccatcccg acaaacaacc cgtgatgtgg
     2041 ttggatgatc atgatggcgt gcggtgagag ccgtgtaccg gaccggccag agggggctcg
     2101 cacccggcgt gctccacgac caccggggga agggtgagcc cgtggcggac ttcatcgccg
     2161 aacgttggca acagatcctc tacaacgcct t
//
LOCUS       DATGUK010000042         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_397683,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000042 DATGUK010000000
VERSION     DATGUK010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397683"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VK103_00655"
     CDS             complement(<1..625)
                     /locus_tag="VK103_00655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HLQ82016.1"
                     /translation="MGPANRLSVVAYGRDLTNPRSHAGQGVLVVAATLAAALSLAGGA
                     GAQILAAVVLTALTGVALTSGVPGGIRACVFLGSVFVVFVLSGLIGVPGPVTTVLVGL
                     LPVTVLLAAAALTVWAAVARPEPPSYARTLQEMLWWIGVIGVAGFALVNPVWEELLFR
                     GVVLQEIAGAWGMRLAVPAQAVLFGAAHWAGFPSGWVGMALAASWGFV"
     gene            complement(679..>2565)
                     /locus_tag="VK103_00660"
     CDS             complement(679..>2565)
                     /locus_tag="VK103_00660"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HLQ82017.1"
                     /translation="GSAARPAAASEPLPRFGSCKKLTDWYSHQALKRARYHRPKPLVQ
                     APEALRTRSSESGDGIVVGSAAQAAETDIGPVGNGDTGTNVQEPGVDEPDITKTDGEV
                     VATLEHGQLAIFDVTGDSPELLSRLGEHFRDADEMLLVGDRLVVFDQSAPVAGATIEP
                     GAPEPSVSEPSVSEPDGAESPHRSHQRTRITVVDIANPDEPRISWSASVDGSYVTARE
                     HDGTVRVALTYRPLLGFVHPDPGVGPAEAAARNRQLARTWQAQDWLPSVHGPEGTQQP
                     LLTCSDVRRPRRPSGLGTTSIVTLDPTSPAQFDATAVAATGTLVYASADRFYVALTDT
                     RSRVEARDDLPDGDQPRRSVTRVHAFDTTGRQTSYVASGAVPGVVPGRWAFSEYDGRL
                     RVASTTMRPVTGTDASGRDSARTQSRVSILIEAHDRLEVVGTVDGLGPTEQIRAVRWF
                     DEIAVVVTFRRTDPVYTLDLSEPSDPRLLGELKITGYSAYLHPVGGDLLLGVGQNASL
                     SGVTLGTQVSSFDLSDLSEPKRVDSVEFGKRSRSPVEQDSRTFTYLPERRLAFVPLST
                     ATGGASHLGVVRVADGGALRLEQTVPVPGPAYQVRVMPIDGDRVAVVANGVVQRLVDP
                     GNLR"
BASE COUNT          396 a          921 c          870 g          378 t
ORIGIN      
        1 ggacgaagcc ccagctggcc gccaacgcca ttccgaccca cccggacggg aaccccgccc
       61 agtgcgccgc cccgaacagc acggcctgcg ccggcaccgc cagtcgcatc ccccaggcgc
      121 ctgcgatctc ctgcaggacg acgccgcgga acagcagctc ctcccagacg gggttgacca
      181 gtgcgaagcc ggcgactccg atcacgccta tccaccacag catctcctgc agagtccgcg
      241 cgtaggacgg cggttccgga cgcgccaccg ctgcccacac tgtgagcgcg gccgcggcga
      301 ggagcacggt caccggcagc aagcccacca gcaccgtggt gacaggaccc ggcaccccga
      361 tcagtcccga cagcacgaac accacaaaca cggagccgag gaacacgcag gcgcggatac
      421 ctccgggcac gccgctggtg agggcaaccc cagtcagcgc ggtgagaacc accgctgcca
      481 ggatctgcgc ccccgcgccg cccgcgaggc tgagcgcggc ggccagcgtg gccgccacaa
      541 ccagcacacc ctgcccggcg tggctacgcg ggttggtgag gtcccgcccg tacgccacca
      601 cggacagtcg gttggcggga cccacgcccg catgctgtca caccctcgag tgcccgggca
      661 ccagatttcg ccgggcaatc agcggagatt tccggggtcg acgagccgct ggaccacacc
      721 gttggcgacg actgccaccc ggtcgccgtc gatcggcatc acccgcacct ggtacgcggg
      781 acccggcacc ggcacggtct gttccagccg cagcgccccg ccgtcggcga cgcgcaccac
      841 cccgaggtgc gacgctccgc cggttgcggt gctcaatggc acgaaagcca gccggcgttc
      901 gggaaggtag gtgaacgtcc gggagtcctg ctccactgga ctcctggagc gcttgccgaa
      961 ctcgaccgag tccacccgtt tcggctcgct caagtctgac aggtcgaagc tggacacctg
     1021 agttcccaac gtgacccccg acagactcgc gttctgcccg accccgagga gcaggtcccc
     1081 gccgaccggg tgcaggtacg ccgagtagcc ggtgatcttc aactcgccga gcagtctcgg
     1141 gtcacttggc tcggacaggt ccaacgtgta caccgggtcg gtccggcgga aggtcaccac
     1201 cacggcgatc tcgtcgaacc agcgcaccgc gcggatctgc tcggttgggc cgaggccgtc
     1261 cacggtgccg acaacctcga gccggtcgtg ggcctcgatc aggatgctca cccgggactg
     1321 ggtccgcgct gagtcccgcc cgctcgcatc cgtgccggtc accggccgca tggtggtgct
     1381 ggcaacccgc agccgtccgt cgtactcgga gaacgcccac cggcccggaa cgactcctgg
     1441 cacggctccc gacgccacgt atgatgtctg ccgtcccgtg gtgtcgaagg cgtgcacccg
     1501 ggtgacactc ctcctcggct ggtcgccgtc tggcaggtcg tctcgggcct caacccggga
     1561 ccgggtgtcc gtcagcgcga cgtagaagcg gtcggccgag gcgtagacga gtgtcccggt
     1621 cgccgccacc gcggtcgcgt cgaactgcgc cgggctcgtc gggtcaagcg tgacgatcga
     1681 cgtggttccg agcccggacg ggcgtcgtgg gcgtcggacg tcggaacagg tcagcagcgg
     1741 ctgttgtgtg ccttcgggtc cgtggaccga cgggagccag tcttgggcct gccaggtacg
     1801 ggccagctgc cggttcctag cagcggcctc ggccggcccg acaccggggt cggggtggac
     1861 gaatcccagc agcggccggt aggtgagggc gactcgtacg gtgccgtcgt gctcgcgtgc
     1921 ggtgacgtac gagccgtcga ccgaggccga ccaggagatc cgcggctcgt cggggttcgc
     1981 gatgtcgacg accgtgatcc gggtgcgctg gtggctccgg tgcggtgact cagcgccatc
     2041 tggttcggag acgctgggtt cggagacgct gggttcgggg gctccgggct cgatggttgc
     2101 cccggcgacc ggggcgctct ggtcgaacac caccagacgg tcaccgacga gcagcatctc
     2161 gtcggcgtcc ctgaagtgct cgcccagccg cgagagcagt tccgggctgt caccggtcac
     2221 gtcgaagatc gcaagctgcc cgtgctcgag ggttgccacg acctcgccgt cggttttcgt
     2281 gatgtccggt tcgtcgacgc caggttcctg cacgttcgtt ccggtatcac cgttgccgac
     2341 cggcccaata tcggtctcag cggcttgagc ggcgctgcct acgacgatac cgtccccgga
     2401 ctctgacgac cgggttcgca gcgcctcggg cgcctggacc aacggcttcg ggcggtgata
     2461 cctggcacgt ttcagcgcct ggtgggaata ccagtccgtg agcttcttgc aactgccgaa
     2521 cctgggaagc ggttccgagg ctgcggcggg gcgagcagca ctgcc
//
LOCUS       DATGUK010000043         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_399125,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000043 DATGUK010000000
VERSION     DATGUK010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399125"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="VK103_00665"
     CDS             <1..1733
                     /locus_tag="VK103_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82018.1"
                     /translation="TKKAYIRGMAWDSARQDVIISTGPTDPAIWRVDTETRQAVRLTN
                     DDRQPGLSEEAFINAVTVIGDRIIARSYTTQQLLVIDRDGNTEHWAEDDRVHIWGHRF
                     IPHPTDPDTMLFTNRTELWRYSFSQRQVEPTGLDVGGYISTAFPTPADANTLAGVASN
                     GTFLIDLATGSTPELTLNPYTFALPTRLETPLQGPDNTMWAAGPSALAQVDTTAAGTV
                     YPTVRMAREFRSSIIRDGKMYLGAYTGSSFSVFDPTTPTVAPRTLFTAAEVGFDRPNS
                     MTYDPVNDKAYMGSTPGYGLNQGGISVWDFTTSSVTHFRSEVISEQGVGSVVHSPSDG
                     MIYIGSNVDGGNGHPDSGQTEAYLITWDPTTDTKVGQLVPVADRRGITGLAVGPDGLV
                     WGIAEDTVFLYSPATGQVERTIRVLSQGYGDGNVWPWGYTEYSAKDAMMYASLGQRMV
                     RIEPRTFEVTDVGIGRGSRVRVDGDGNVYFLATFESEHLLRYTPDTVDRRATVWIGSV
                     DTGVPNHEVDRGHTVNDLIDDDRDWQSHSAFVRHVRDVTHTLVDRGVLTVREASTITG
                     AAGRSTIGRK"
     gene            1742..2791
                     /locus_tag="VK103_00670"
     CDS             1742..2791
                     /locus_tag="VK103_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82019.1"
                     /translation="MPEPVIHDDTVQQRNRRYQREVEDYFRSHLVDQYRDRADRAWVR
                     DHSSNEQYLRSVEPMRREWRRLLAPPDLVAAEPCLTTSGWLPDIDSWWLSLPLAGGLT
                     AQAGLAVPSGGASGLVVFQHGLASYPERAFGVGDDSGAYDDVGHQLLEAGFAVLAPLN
                     LSFRGPRNRAQRLARLAGTTMEGLELARVRLLLAEAEEVAGPFGPVGLWGCSWGGMAT
                     QFWTPLDDRFRVAITTAFFNHRPNKMVVQDTRYGTFEDSGEDHAFLHGHLTHFSDADL
                     ASLVCPRPFQVQIGKADAIGWWPQIEGEFRLARDHWDRLGVGERVALDLHEGGHCVRG
                     AAGVAWLTKWLTDQP"
BASE COUNT          533 a         1057 c          962 g          453 t
ORIGIN      
        1 cgacgaagaa ggcgtacatc cggggcatgg cctgggacag cgcccgtcag gacgtcatca
       61 tctccacggg tccgaccgat ccggccatct ggcgggtcga caccgagacc cgacaggcgg
      121 tgcggctgac caatgacgac cgacagcccg ggttgtcgga ggaagccttc atcaacgcgg
      181 tgaccgtcat cggggaccgc atcatcgctc gttcctacac cacccaacag ctgctcgtca
      241 tcgaccgcga cgggaacacc gagcactggg ctgaggacga tcgcgtgcac atctggggcc
      301 accgcttcat cccgcaccca acagatcccg acacgatgct gttcactaac cggaccgagc
      361 tgtggcgcta cagcttcagt cagcgtcagg tcgagccaac aggcctcgac gtcggcggct
      421 acatcagcac cgcattcccg accccggccg acgcaaacac tcttgccggt gtcgccagca
      481 acggcacctt cctcatcgac ctcgcaaccg ggagcacccc ggagctgacg ttgaacccgt
      541 acacgttcgc cctgccaacc cgactggaga ccccactgca gggtccggac aacacgatgt
      601 gggcagccgg acccagcgcg ttggcacagg tcgacaccac ggcggccggg accgtctacc
      661 cgacggtccg gatggcccgg gagttccggt cgtccatcat ccgcgacggc aagatgtatc
      721 tgggcgcgta caccgggagc tcattcagcg tgttcgatcc gacgaccccc acagtggcac
      781 cgcgcaccct cttcaccgcc gcggaggtcg gtttcgaccg gcccaactcg atgacctatg
      841 acccagtgaa cgacaaggcc tacatggggt ccaccccagg ctacgggctg aaccagggcg
      901 gcatctcggt gtgggacttc accaccagca gcgtcacgca cttccggagc gaggtgatct
      961 ccgagcaggg cgtcggctcg gtggtccaca gcccctccga cggcatgatc tacatcggca
     1021 gcaacgtcga cgggggcaac gggcaccccg acagcggcca gacggaggcg tacctgatca
     1081 cctgggaccc cacgacagac accaaggtcg gtcaactggt ccccgtggcc gaccggcgtg
     1141 gcatcaccgg gctggccgtc gggcctgacg gcctggtctg gggcatcgcc gaagacacgg
     1201 tgttcctcta cagtccggcg accggtcagg tcgagcgtac gatccgggtg ctcagccagg
     1261 ggtacggcga cggaaacgtg tggccatggg gctacaccga gtactcggcg aaggacgcca
     1321 tgatgtatgc ctcgctgggc cagcgcatgg tgcggatcga gccccgcacg ttcgaggtca
     1381 ccgatgtggg aatcggccgc gggtcccgcg tacgggtgga cggggacggc aacgtgtact
     1441 tcttggccac cttcgagtcc gagcacctgt tgcggtacac acccgacacc gtcgatcgcc
     1501 gggccaccgt gtggatcggc tccgtcgaca cgggggtgcc gaaccacgag gtcgacaggg
     1561 ggcacacggt caatgacctg atcgatgacg accgagactg gcagagccac agcgcgttcg
     1621 tccggcacgt cagggatgtc acccacaccc tggtcgaccg cggcgtcctc accgtacgcg
     1681 aggccagcac gatcacgggg gcagcgggac gatcaaccat aggcaggaag taggaggcac
     1741 tgtgccggaa ccagtgatac atgacgacac ggtccaacag cgcaaccggc ggtaccagcg
     1801 tgaggtggag gactacttcc gcagccactt ggtcgaccaa tatcgtgacc gcgccgaccg
     1861 cgcctgggtg cgcgaccact cgtcgaacga gcagtacctg cggagcgtgg agcccatgcg
     1921 gagggagtgg cgacggctac ttgcaccccc agacctggtg gctgcggagc cgtgcctgac
     1981 cacctccggc tggctgcccg acatcgactc ctggtggttg tcgctcccgc tcgccggtgg
     2041 cctcaccgcc caggccggac tcgccgtgcc cagcggcggc gcatccggcc tggtggtgtt
     2101 ccagcacgga ctcgcgtcct accccgagcg cgctttcgga gtcggtgatg actcgggtgc
     2161 ctacgacgac gtcggacacc agctgctcga ggccggtttc gccgtcctcg cgcccttgaa
     2221 cctcagcttc cgcggaccgc ggaaccgggc ccagcgactc gcacgcctcg caggaaccac
     2281 gatggagggc ctcgaacttg cccgggtacg cctgttgctc gccgaggccg aggaggtggc
     2341 cggcccgttc gggcccgtcg ggctgtgggg atgcagctgg ggcggcatgg ccacccagtt
     2401 ctggacgccg ctcgatgacc gctttcgggt cgcgatcacc accgccttct tcaatcaccg
     2461 ccccaacaag atggtcgtcc aggacacccg gtacgggacc ttcgaggaca gcggggagga
     2521 ccacgcattc ctgcacggac acctcaccca cttctccgac gccgacctgg cctccctggt
     2581 ctgccctcgt ccgttccagg tgcagatcgg caaggccgac gcgatcggct ggtggccgca
     2641 gatcgagggt gagttccgtc tcgcccggga ccactgggac cgtctgggag tcggtgagcg
     2701 cgtggcactg gacctccacg agggcggtca ctgcgtccgg ggcgccgccg gcgtggcctg
     2761 gttgacgaaa tggctgacgg accaaccctg atgtaacaac catgcaaccc gttgcgtatc
     2821 cgatgctcga tcgccgaaca tggatcacgc ctcgggcaag gcagctcagg catcccgtac
     2881 caggagttga tcgtgcacgt tcatcgtcga accgtcctcg gcgccctcgc cctcaccccg
     2941 gcactggctg ccttgcccag cctgcccgcc accaccgccc gtgccgacgt cggcgcggcc
     3001 agcac
//
LOCUS       DATGUK010000044         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1216736,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000044 DATGUK010000000
VERSION     DATGUK010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216736"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VK103_00675"
     CDS             complement(<1..1168)
                     /locus_tag="VK103_00675"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HLQ82020.1"
                     /translation="MTSTRGAAPDRASPSTVRWGICAVVICLVTLIAALLYGGGAGGS
                     AIGGITSPGDLTRWGLPVTRTLLDVSGAVTVGLLGLAMAVPLRDGRLDPDGMRAFHAA
                     TWSSVVLAVAAGCTHLFTLSDLVGRPLPDALAGDSFASFTAEVAEGRAYAAVVVIALT
                     VTAATRLVVTRGGIVATFVLALTLWVPLGLVGHAASGDYHHSAVVSLLFHLIGMSVWI
                     GGLVSVVWYASVKGRHLVPVVRMYSLAALGCFLLVGLSGLFNGLVRIFDIADLFTTGY
                     GQLMLVKVVVYAALCWFGWQHRERTIPSLEAGERHSFLRLATGEMVVIGTAVAVGVAL
                     GRTPPPPEESGTSLVRTLIGTPPPGPIDAAGLFGTFYPDSLFGIGILLAGGLYVA"
     gene            complement(1168..1857)
                     /locus_tag="VK103_00680"
     CDS             complement(1168..1857)
                     /locus_tag="VK103_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HLQ82021.1"
                     /translation="MHARASTQFGRSLRVVLVALGLLAMGAITTTAAYAHDSLIDSTP
                     GDGEALQEAPSEVELVFSGNIGTDFAQVSVMSADGTDHADGEPVIEGDTLTQPVSDMA
                     EGEYEVGFRIVSSDGHPVEDSLTFSVASAAGGGDETPEATDQAQSGTEATEQATEQAE
                     PDEESTEDTTPAAGSAADESDDQGPMPWAIGALVAILVVIGIVGLLIRRKRRSESSTS
                     PGSQGSPDNTA"
BASE COUNT          363 a          698 c          701 g          289 t
ORIGIN      
        1 ccgccacata cagcccgccc gccagcagga tcccgatgcc gaacagcgag tctgggtaga
       61 acgtgccaaa caggccggcg gcgtcgatcg ggccgggggg tggagtcccg atcagcgtgc
      121 gcaccagcga cgtgccggac tcctcaggtg gcggtggtgt gcggcccagg gccacgccga
      181 cggccaccgc ggttccgatg accaccatct cgccggtcgc gagtcgtagg aagctgtgcc
      241 gctccccggc ttcgaggctg ggtatcgtcc gctcacggtg ttgccagccg aaccagcaca
      301 gcgccgcgta caccacgacc ttgaccagca tcagctgtcc gtacccggtg gtgaacaggt
      361 cggcgatatc gaagattcgg accagtccgt tgaacagacc gctgaggccg accagcagga
      421 agcagccgag cgccgcgagg gagtacatgc gcacgacggg gaccagatgc ctgcccttga
      481 cggatgcgta ccagacgacc gacaccagcc cgccgatcca aacggacatt ccgatcaggt
      541 ggaacaacag cgataccacc gcggagtggt ggtagtcacc ggaagccgcg tggccgacga
      601 gcccgagggg cacccagagg gtcagcgcca agacgaacgt cgccacgatc ccgccacgag
      661 tgacgacgag gcgcgtcgcg gccgtgaccg tcagcgcgat gacgacgacg gcggcgtagg
      721 cacggccttc ggccacctcg gccgtgaaac tggcgaagct gtcgccggcc agggcatccg
      781 gcagcggccg accgaccagg tccgacaggg tgaacaggtg ggtgcagccc gcagccacgg
      841 ccagtacgac gctcgaccac gtcgccgcgt ggaacgcgcg catcccgtcg gggtccagcc
      901 gcccgtctcg taggggcacg gccatggcca ggccgagcag accgaccgtg acggcgccgg
      961 agacgtcgag cagcgtacga gtgaccggca gaccccagcg agtgaggtcg ccgggcgagg
     1021 tgatgccgcc gatggcggaa cctccggcac caccgccgta cagcagcgcc gcgatgagcg
     1081 tcaccaggca gatgactacc gcgcagatgc cccagcggac cgtggacgga cttgcacggt
     1141 ccggggcagc accgcgggtc gaggtcatca ggcagtgttg tctgggctgc cctgactgcc
     1201 cgggctggtg gagctctcgg aacgacgctt gcggcggatc agcagaccga cgatcccgat
     1261 gaccaccaga atggcgacga gggcgccgat ggcccatggc atcgggcctt ggtcgtcgga
     1321 ctcgtccgcg gcggatccag cggccggtgt cgtgtcctcc gtcgactcct catccggttc
     1381 ggcctgctcg gtggcctgct cggtggcctc cgtgcccgac tgtgcctggt cagtggcctc
     1441 cggcgtttca tcaccgccgc cggcggcgga cgcaaccgag aatgtgagcg agtcctcgac
     1501 cgggtggccg tcgctggaga caatccggaa gccgacttcg tactcgccct cggccatgtc
     1561 cgagaccggc tgggtcaggg tgtccccttc gatcaccggc tcgccatcgg cgtgatcggt
     1621 accatccgcg ctcatcaccg agacctgcgc gaagtcggta ccgatgttcc cgctgaacac
     1681 gagttccacc tcagacggcg cttcctgtaa cgcctcaccg tcccccggcg tggagtcgat
     1741 cagggagtcg tgcgcatacg ccgccgtcgt cgtgatcgcg cccatggcga gcagtccaag
     1801 ggcgaccagg acaactcgga ggctgcggcc gaactgggtg ctggcacggg catgcatcgc
     1861 tggtccttct gcgggtgtgg ggagtccatg agtgagcact accaggctag accaccgggt
     1921 ctcgcggcac tgtcaacacc tgacgtagag cagctgcccg ccggtacgtt cagtggcgtc
     1981 accgatccga gcgcgctgac ggatgtcgtg ggtgatgcaa acccatagcg tcgcatcgaa
     2041 agcccgcgac a
//
LOCUS       DATGUK010000045         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_401471,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000045 DATGUK010000000
VERSION     DATGUK010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401471"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2049)
                     /locus_tag="VK103_00685"
     CDS             complement(<1..2049)
                     /locus_tag="VK103_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HLQ82022.1"
                     /translation="MEVARAGSPAADAVLDCAELGLDGAAADAEHRFDSVDPWTAETP
                     RLYEVQVRTPGETVTVRVGFRRVEVVDAEVLVNGRPILLRGMNRHEFDPDHGRTVSPE
                     RTREELLIMKRHNVNAIRTSHYPPAPWFCDLADELGFWVMLENDLETHGFYAYGYGGD
                     PTEDPTWQDALVDRMQRTVERDKNHPSIFCWSLGNEAGDGDNLITMAEWTKERDPDRL
                     VHYERDQRSGAVDLWSQMYTSHEKLATIAAFQEEALDDPRLDGHRRNLPFVLCEYGHA
                     LGTGPGGLTEYQELFEQSRRLVGGFIWEWIDHGIRTTDEQGREFFGYGGDFGEPLHDG
                     AFVTDGCVFPDLTPKPALVDFAKVVEPVRITVEDDQVRIVNRYDHTDTDHLDFVWQQG
                     RKQGVLSVPVLTPGEEALVALPRRAGRGTLTVSAVLAVDTAWAAAGHEVAWGQQIAVV
                     EPPEVTPKRPTRWARDRVLGAGRFDAHGMLVELAGVPVSGPRLVLDRAPTDNDLKQGW
                     PHVGDGSAADIFARQGLSLLRPRLVSLTADDEGVTTVHRWGGIGRAAAVRTTMRWTTD
                     GRRLALVADAVPEGEWGEYWGRIGLEFVLDKKVPSVAWTGAGPGQAYPDCGQAARHGE
                     WTATVDDLQVPYLRPQECGARTEVSALTLAKGFAIAGEPFAFTVRPWSTAELSAAAHP
                     T"
BASE COUNT          316 a          785 c          663 g          321 t
ORIGIN      
        1 ggtgggatgc gcagcagcgg acagctcggc ggtcgaccac ggccgtacgg tgaacgcgaa
       61 cggttcaccc gcgatcgcga agcccttggc caaggtgagc gccgacacct ctgtacgggc
      121 accgcactcc tgcgggcgca ggtacggcac ctgcaggtcg tccacggtgg cggtccactc
      181 gccgtgccgg gccgcctgac cacagtccgg gtacgcctgc cccgggccgg cgccggtcca
      241 cgcgaccgac gggaccttct tgtccaacac gaactccagg ccgatccgcc cccagtactc
      301 accccactca ccctcgggta cggcgtcggc gaccagtgcg agccgccggc cgtcggtcgt
      361 ccaccgcatc gtcgtgcgta ctgcagcggc tcggccgatc ccgccccacc ggtgcacggt
      421 cgtgacgccc tcgtcgtcgg cggtcagcga caccagccgc ggccgcagca acgagagccc
      481 ttgtcgggcg aagatgtcgg ccgccgaccc gtcaccgacg tgcggccagc cctgcttgag
      541 gtcgttgtcg gtgggggcac gatccagcac cagccgcggt ccggacaccg gcacgccggc
      601 caactcaacc agcatcccgt gggcgtcgaa ccgaccggca ccgagcacgc ggtcccgggc
      661 ccaacgggtc ggccgcttgg gcgtgacctc cggtggttcc acgacggcga tctgttgccc
      721 ccatgccacc tcgtggccgg cagccgccca cgccgtgtcg actgcgagca cggcgctcac
      781 ggtcagtgtt cctcgcccgg cccgccgagg cagcgcaacc agcgcctcct caccgggcgt
      841 cagcaccggc actgacagca cgccctgctt gcggccctgt tgccacacga agtccaggtg
      901 gtcggtgtcg gtgtggtcgt agcggttgac gatgcggacc tggtcgtcct cgacggtgat
      961 gcggaccggt tccacgacct tggcgaagtc gaccagggct ggcttcggtg tcaggtcggg
     1021 gaacacgcag ccgtcggtga cgaacgcccc gtcgtgcagt ggttcgccga agtcgccgcc
     1081 gtagccgaag aactcccggc cctgctcgtc ggtggtgcgg atgccgtggt cgatccactc
     1141 ccagatgaac ccgccgacca gccgccggga ctgctcgaac aactcctggt actcggtcaa
     1201 tccaccggga ccggtgccga gcgcatgccc gtactcacac agcacgaatg gcaggttgcg
     1261 ccggtgcccg tcgagccgag gatcatccag cgcctcctcc tggaaggctg cgatggtcgc
     1321 caacttctca tggctggtgt acatctgcga ccacaggtcg accgcaccgc tgcgctggtc
     1381 acgctcgtag tgcaccagcc ggtcggggtc gcgctccttg gtccactccg ccatcgtgat
     1441 caggttgtcg ccgtcaccgg cctcgttgcc gagcgaccag cagaagatcg acggatggtt
     1501 cttgtcgcgc tccacggtcc gctgcatccg gtccaccaac gcatcctgcc aagtcgggtc
     1561 ctcggtcgga tcgccgccgt agccgtaggc gtagaacccg tgcgtctcca ggtcgttctc
     1621 caacatcacc cagaagccca gctcgtcggc gaggtcgcag aaccacggcg ccgggggata
     1681 gtgcgaggtc cggatcgcgt tcacgttgtg ccgcttcatg atcagcagct cctcgcgggt
     1741 gcgttccggc gacaccgtcc gaccatgatc aggatcgaat tcgtgccggt tcatcccgcg
     1801 cagcaggatc ggtcgcccgt tcaccagcac ctctgcatcg acgacctcca cgcggcggaa
     1861 cccgacccgg accgtcaccg tctcgcccgg ggtgcgcacc tgcacctcgt acagccgtgg
     1921 cgtctccgcg gtccacggat ccaccgagtc gaaccggtgc tcggcatcgg cagccgcccc
     1981 gtccagcccg agctccgcac agtccagtac ggcgtcggcc gccggcgacc cggcacgcgc
     2041 cacctccacc cgcaacgagc ccgacccgga ctcgtggtca tagtc
//
LOCUS       DATGUK010000046         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1337815,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000046 DATGUK010000000
VERSION     DATGUK010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337815"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VK103_00690"
     CDS             <1..1011
                     /locus_tag="VK103_00690"
                     /inference="COORDINATES: protein motif:HMM:NF036726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HLQ82023.1"
                     /translation="HAFPPQVPPAPQGDPTPPADPDRWLVERLTGDPELLGRLGFAGQ
                     DLADARTAIADLLADDSDTGRADRAHVLDLALRIPGTVGRFVWHSDVFGDKEAEARAT
                     AAIPGHGWGVLALCALVATVPEVQRFHRSRDITEEMSWEALADIGRQVRVHRDTFDEF
                     GLHAHGWLLCIWSGALYKFGRLQFNLQWYQPQEPTPDRPVADPTPRWVLSTHIPETGP
                     LTPEAVAASFRQGRQFFADHFPDYPTREFFCGSWLLDPRMARLAPESNTARFQALWQL
                     DGPGTDANGDAFFFLWRRRGEVDPASLPADSSLRRLVVGEVLAGRPWQVCRGLIPYDD
                     VD"
     gene            complement(1031..1924)
                     /locus_tag="VK103_00695"
     CDS             complement(1031..1924)
                     /locus_tag="VK103_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82024.1"
                     /translation="MYTVLAVTLAVIGSACNAWASVLQHHVVDTTVSNDLLDHSRKMS
                     PRRMITLLHSPRWWFAISMVVLGAVFHIIGLALAPITLVQPIGVLGLVFALLIASRHR
                     RSWPGGRVWAGAGITVIGIAAFVVVAADQVSHTEDLHRGDIVWVGLLLLLLAAGFGVV
                     GAVGPAWLRCLAWAAAASTVFGLASALLRTVTVLIGADTPLLSPIMLGLGGALAVCYV
                     VGGWLAQQAYASGPPETVMACLTVVDPLVAVGFGIVVLREGVGITTGGGVAMAAAGLV
                     AATGAVVLARHHPDATKAYSG"
     gene            2050..2616
                     /locus_tag="VK103_00700"
     CDS             2050..2616
                     /locus_tag="VK103_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLQ82025.1"
                     /translation="MTELHLVRHGETEWSANGRHTSSTDLDLTDNGVAEATLLRGRLD
                     PKAYQLVLSSPLLRARRTAELAGFGAPEIDPDLHEWRYGDYEGLTTPQIREHDPGWTV
                     WTHTSPGGETADEVSRRLTRVIARIRESGVERALCFAHGHALRALTLTWLGLDLTLGD
                     RFPLQTGTVSVLGDYKDGQALISWNARP"
     gene            2770..3423
                     /locus_tag="VK103_00705"
     CDS             2770..3423
                     /locus_tag="VK103_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HLQ82026.1"
                     /translation="MPEQAELKALAADHSRVSEELVGAETRVSDLELAVAKAESDLTP
                     VRERRERDQQRIADGSVTDPKALETMIDEVEHLTRRIGDLEDVQLEVMEEHEQAVAER
                     DQLAARKAEIAGNGTRARTRRDEVVGEIDAEVTERTSDREALAVTLPDDLLALYDKLR
                     TGRGGVGAAELRQRRCTGCQIEANAADLRSYATAAEDEVLRCEECGRILVRTEESGL"
BASE COUNT          538 a         1244 c         1259 g          426 t
ORIGIN      
        1 catgcgttcc cgccgcaggt gcccccggcg ccgcagggcg acccgacacc gcctgccgat
       61 cctgaccggt ggctggtcga gcggctcacc ggcgacccgg agctgctcgg tcgtctcggc
      121 tttgccggtc aggacctggc cgatgcccgc accgcgatcg ccgacctgct cgccgacgac
      181 agcgacaccg gacgggccga ccgggcccac gtgctcgacc tggcgttgcg gatccccggc
      241 accgtcggcc ggttcgtgtg gcactccgac gtgttcggcg acaaggaggc cgaggcccgt
      301 gccaccgcag cgatcccggg acacggctgg ggcgtgctgg cgctgtgcgc gctggtcgcc
      361 actgtcccgg aggtgcagcg gttccaccgg tcgcgcgaca tcaccgagga gatgtcgtgg
      421 gaggcgctgg ccgacatcgg ccgacaggtc cgggtgcacc gcgacacctt cgacgagttc
      481 ggcctgcacg cccacggctg gctgctgtgc atctggtccg gggcgctgta caagttcggc
      541 cggctgcagt tcaacctgca gtggtaccag ccgcaggaac cgacaccgga ccgtccggtc
      601 gccgacccga ccccgcgctg ggtgctgtcg acccacatcc ccgagaccgg gccgctcacc
      661 cccgaggcgg tggcggcgtc gttccggcag gggcggcagt tcttcgccga ccacttcccg
      721 gactatccga cccgggagtt cttctgcggc tcctggctgc tcgacccgcg gatggcgcgg
      781 ctggcgccgg agtccaacac cgcccggttc caagcgctgt ggcagctcga cgggcccggc
      841 accgacgcca acggcgacgc cttcttcttc ctgtggcggc gccgcgggga ggtcgacccg
      901 gcgagcctgc cggccgactc gtcactgcga cggctggtgg tcggtgaggt gctcgccgga
      961 cggccgtggc aggtctgccg cgggctgatc ccgtacgacg acgtcgactg agcggccggg
     1021 cccgaccggc tcaacccgag tacgccttcg tcgcgtccgg gtggtgccgg gcgagcacca
     1081 cggccccggt cgccgcgacc aggccggcgg ccgccatcgc gaccccgccg ccggtggtga
     1141 tgccgacacc ctcccgcagc accacgatcc cgaaaccgac cgccaccagc ggatccacca
     1201 ccgtcagaca ggccatcacg gtctccggcg gcccggaagc gtacgcctgc tgggccagcc
     1261 atccgccgac cacgtagcag accgcgagcg cgccgccgag gccgagcatg atcgggctca
     1321 gcaggggcgt gtccgccccg atcagcacgg tgacggtgcg cagcagggcc gaagccaacc
     1381 cgaacacggt ggaggccgca gccgcccagg ccaggcagcg cagccaggcc ggaccgaccg
     1441 cgccgacgac gccgaacccg gccgcgagca gcagcaacag caacccgacc cagacgatgt
     1501 cgccgcggtg caggtcctcg gtgtggctga cctggtcggc cgccaccacc acgaaggcgg
     1561 cgatcccgat caccgtgatg cccgcccccg cccacacccg gccacccggc caggagcggc
     1621 ggtgccggga cgcgatcagc agggcgaaca ccaatcccag cacgccgatc ggctgcacca
     1681 acgtgatcgg ggccagcgcc agtccgatga tgtggaacac cgcgcccagc acgaccatcg
     1741 agatcgcgaa ccaccagcga ggactgtgca gcagggtgat catccgccgc ggcgacatct
     1801 tgcgggagtg gtcgagcagg tcgttgctca cggtcgtgtc gacgacgtgg tgctgcaaca
     1861 ccgacgccca ggcgttgcag gccgacccga tgacggcgag cgtgacggcg agcacggtgt
     1921 acaccgatgc gggtcctctc ggccggtccg gacgggcggc ggcccggcgg accgcctgcc
     1981 ccactgtatg acccggctcg gggatgccag actggagcgc agttcccacg agctgcggag
     2041 aggacgagga tgaccgagct ccatctggtg cgccacggcg agacggagtg gagcgcgaac
     2101 ggccgccaca cctccagcac cgacctcgac ctcactgaca atggggtcgc cgaggcgacc
     2161 ctgctgcgcg ggcggctgga cccgaaggcg taccagctgg tgttgtcgag cccgttgctg
     2221 cgggcccgtc gcaccgcgga gctggccggc ttcggcgcgc cggagatcga tcccgacctg
     2281 cacgagtggc gctacggcga ctacgagggg ctcaccactc cgcagatccg cgagcacgac
     2341 ccgggctgga cggtgtggac ccatacctcg ccgggcgggg agaccgctga cgaggtgtcc
     2401 cggcggttga cccgggtgat cgcgcggatc cgtgagtccg gggtggagcg ggcgctgtgc
     2461 ttcgcccacg ggcacgcgct gcgggcgctc accctcacct ggctcgggct ggacctgacc
     2521 ctcggtgaca ggttcccgct gcagaccggc accgtatcgg tgctcggcga ctacaaggac
     2581 ggccaggcgc tgatcagctg gaacgctcgg ccgtgagccg gggcatccgg tggtggccgg
     2641 taggctggtc gcggcacatt cgcgggagag gggtctgaca tcagagcgac accggccgac
     2701 caggccacac tgcttgagct gcaggcggtc gacaccgcgc tcgaccagct ccgccaccgg
     2761 cgacagaact tgcccgaaca ggctgagctg aaggcgctcg cggccgatca ctcccgggtg
     2821 tcggaggagc tggtgggagc cgagacccgg gtcagcgact tggagctcgc cgtagcgaag
     2881 gccgagtccg acctcactcc ggtgcgggag cgccgcgagc gcgaccagca gcggatcgcc
     2941 gacggctcgg tgaccgaccc gaaggcactg gagaccatga tcgacgaggt cgagcacctc
     3001 acccgccgca tcggcgacct ggaggacgtc cagctcgagg tgatggagga gcacgagcag
     3061 gcggtggccg agcgcgacca gctggccgca cggaaggccg agatcgccgg caacggtacc
     3121 cgcgcgcgca cccggcggga cgaggtggtc ggcgagatcg acgccgaggt caccgaacgc
     3181 acctctgacc gggaggcgct ggccgtcacc ctgccggatg acctgctggc gctgtacgac
     3241 aaactgcgga ccgggcgcgg aggcgtgggt gcggcggagt tgcggcagcg gcgctgcacc
     3301 gggtgccaga tcgaggccaa cgcggcggac ctgcgcagct atgccaccgc cgctgaggac
     3361 gaggtgctgc gctgcgagga gtgcggccgg atcctggtgc gcaccgagga gtccgggctg
     3421 tgagcggtgg ggcttcgacc cggcggcaca gccaagcggg ggaggat
//
LOCUS       DATGUK010000047         4702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_57022,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000047 DATGUK010000000
VERSION     DATGUK010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57022"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2313)
                     /locus_tag="VK103_00710"
     CDS             complement(<1..2313)
                     /locus_tag="VK103_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82027.1"
                     /translation="MRRRTFTGSAVAVAAGFAVPAVGAGTVPDAVGAPTDTAPDPTAD
                     PVTDLGEPVHKVQTLASAIGTTPDGVPFACYVVSGNENVTAEFTVVDLRSQQTLFSTR
                     VPYGRSSQRALALSPVDGSVWFATSDIGHVYHYVHGADEVRHIVEIPNGELAWSMAVG
                     EDDTVWFGTYPSGSLYSLPPDTRELVHHGQALAGEQYVDSIAPVGDTVHVGTQPNARM
                     ATLDRGTGAFTELAMPPGHSGTAVSALDVRGGKLFVSSTQLYVLDLASGDWVDTIPDA
                     DANVSPVDPLDPDTVYLRQAAEVRAYSLSTGQLTGTGDRPNATPESWGWVDLDGTGER
                     PLLALTYWREGRTYGFAVHGGSGFYLVPDLMGAGAPLTALGTGPLGNVFAGAFLSPPG
                     MGRYDPDGDGSIDLLAGTSQVEGFGTFDGRLVFGRYPQGSLYLYDPAQPWNSGSNPAA
                     PLEIGDGQSRPQLFVELSDDPGTVAVGSVPVPGEHGGAITLWRPDDRTVEVHRHVVPD
                     QTPVGLVLHNGMLYGGTSIQGGYGIDPVTDEAVLFAWDPKQRTTTWTTVAVPGAQTLS
                     GLAIADDGHLWGIADGRTVFEFDLAGRALLRTVTIDPEAAIDRYGDNSRLLFDHGRLF
                     GATADRLFVLDRVTGEVTSLYGRGSGRDDGVDSVYELTRDRHGDLYVVGRGTHLVRYA
                     LPADVTPPTVTVRTGRGGQDGGHGGGRGAAVWLQAEDDTDPAPRIQVRLDDGPWQDHD
                     PDRMVLVRPGRHLRYRAVDAAWNASPVQTYT"
     gene            2501..2860
                     /locus_tag="VK103_00715"
     CDS             2501..2860
                     /locus_tag="VK103_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007271715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82028.1"
                     /translation="MDVVTHVFVALHLLSMAAIVGLWFAHFRTPTVTRGQLVGACGSL
                     VTGLALTGFAEMAATADDPVNHAKIGVKAVLAVVVLVAAIIGVRKARTGGQVPTGLAH
                     AVGGTAVINVLIATLWM"
     gene            2919..3929
                     /locus_tag="VK103_00720"
     CDS             2919..3929
                     /locus_tag="VK103_00720"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HLQ82029.1"
                     /translation="MTIHRLLVACTDGVRQLHLDTTTGTAETVGAVLPYGTSLAVTAH
                     PATPGAGWIASADDGGQLVAVTATDDGVRVETSTGSNGAVPCHVAVPDGQVAVANYSG
                     HTVAVFPYHHDSGHRTAGPAVVHHFGEQSHPHQVLEVDGQLVVSDLGRDGLHVLAPDA
                     APAPADGPPAIPLEPGAGPRNAVRVGPDHLVVALERANAAAVVELQRDAAGTVVGGRQ
                     VDRCGFDGETSATHPSQILLAGVEGDTAQVLVLNRGSDRLCVLEVTDGRFGGKAVEHP
                     LPAWPMDLLRLDDVLLVACRDADLLVGLDAADLGSDGAARTLFSVPVPQPNGVASVGN
                     VA"
     gene            3926..4492
                     /locus_tag="VK103_00725"
     CDS             3926..4492
                     /locus_tag="VK103_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010549320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLQ82030.1"
                     /translation="MSALGDRLRSLPTFPADLPTLDPSAVPDDPTALLLAWLDEAIAT
                     GERQANAMTLTTVDDEGRPANRTLIVKDVDDRGLHFSSSLGSRKAAHLAVRPYAGMMF
                     FWRPLGRQLEVSGPVVALDADTSAADWRQRPSYDGRDNPDWQVWALQPARYEFLQATL
                     DRRHVRVEYVRTGDGWRHTRLLPSEQPR"
     gene            complement(4511..>4702)
                     /locus_tag="VK103_00730"
     CDS             complement(4511..>4702)
                     /locus_tag="VK103_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006124187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HLQ82031.1"
                     /translation="VTDHWPHGAIARAFGVFDEESGCALRGSFVLDAEGVVRWSVVNA
                     IGDARDMAAHVAAVSQLAG"
BASE COUNT          636 a         1678 c         1738 g          650 t
ORIGIN      
        1 cgtgtaggtc tgcaccggcg aggcgttcca cgccgcgtcg accgcgcggt accgcaggtg
       61 ccgcccgggc cgcaccagca ccatccgatc cgggtcgtgg tcctgccacg ggccatcgtc
      121 gagccggacc tggatccggg gtgccggatc ggtgtcgtcc tcggcctgca accacactgc
      181 ggcaccacgc ccgccaccgt gtcccccgtc ctgcccgccc cggccggtgc gcaccgtcac
      241 cgtcggtggc gtgacgtcgg ccggcaatgc gtaccgcacc aggtgggtgc cgcgaccgac
      301 gacgtacagg tcgccgtgcc ggtcccgggt gagctcgtag acgctgtcca ccccgtcgtc
      361 gcggccgctg ccgcggccgt acaggctggt cacctcgccg gtgacccggt ccagcacgaa
      421 cagccggtcg gcggtcgccc cgaacagtcg gccgtggtcg aacagcagcc gggagttgtc
      481 gccgtaccgg tcgatcgcgg cctcggggtc gatcgtgacg gtgcgtagca gggcccgccc
      541 ggcgaggtcg aactcgaaga cggtgcggcc gtcggcgatc ccccacaggt gtccgtcgtc
      601 ggcgatcgcg agcccggaca gcgtctgcgc gccggggacg gcgaccgtgg tccaggtggt
      661 ggtgcgctgc ttcggatccc aggcgaacag cactgcctcg tcggtgaccg ggtcgatgcc
      721 gtagccgccc tggatggagg tgcctccgta cagcatcccg ttgtgcagca ccagcccgac
      781 cggggtctgg tccggcacca cgtgccggtg cacctcgacg gtgcggtcgt cggggcgcca
      841 cagggtgatc gcgccaccgt gctcaccggg caccggcacc gatccgacgg cgacggtgcc
      901 gggatcgtcg gacagctcga cgaacagttg cggccggctc tgcccgtcac cgatctccag
      961 cggcgccgcc gggttgctgc cggagttcca cggctgtgcg ggatcgtaga ggtacaacga
     1021 gccctgcggg tagcggccga agaccagtcg gccgtcgaac gtgccgaagc cctccacctg
     1081 actggtgccg gcgagcaggt cgatggagcc gtccccgtcc gggtcgtacc ggcccatccc
     1141 gggtgggctg aggaacgcac cggcgaacac gttgccgagc ggtccggtgc cgagcgcggt
     1201 gagcggcgcc ccggcaccca tcaggtccgg gaccaggtag aagcccgagc cgccgtgcac
     1261 cgcgaagccg taggtgcgtc cctcgcgcca gtaggtgagt gcgagcaacg ggcgctcccc
     1321 ggtgccgtcg aggtcgaccc agccccagga ctccggggtg gcgttggggc ggtcgccggt
     1381 gccggtgagc tgtccggtgc tcaacgagta cgcccggacc tccgcggcct gccgcaggta
     1441 gacggtgtcg gggtcgagcg ggtcgaccgg tgagacgttg gcgtcggcat cagggatggt
     1501 gtcgacccag tcaccgctgg ccaggtcgag cacgtacagc tgggtgctgc tgacgaacag
     1561 tttgccgccg cgcacgtcca gcgcgctcac cgcggtcccg gagtggccag ggggcatcgc
     1621 gagctcggtg aaggcgccgg tgccgcggtc gagggtcgcc atccgcgcat tgggctgggt
     1681 gccgacgtgg acggtgtcgc cgaccggggc gatggagtcg acgtactgct caccggccag
     1741 cgcctgcccg tggtggacga gctcgcgggt gtcgggcggc aatgagtaca ggctgccgct
     1801 ggggtaggtg ccgaaccaca cggtgtcgtc ctcgccgacc gccatcgacc aggcgagctc
     1861 gccgttcggg atctcgacga tgtggcggac ctcgtcggcg ccgtggacgt agtggtagac
     1921 gtggccgatg tcggaggtgg cgaaccacac cgagccgtcc accggggaca gcgccagcgc
     1981 ccgctggctg ctgcgcccgt acggcacccg ggtgctgaac agcgtctgct ggctgcgcag
     2041 gtcgaccacg gtgaactcgg cggtgacgtt ctcgttgccc gagaccacgt agcaggcgaa
     2101 cggcacgccg tcgggcgtgg tgccgatcgc ggaggcgagc gtctgcacct tgtgcaccgg
     2161 ctcgccgagg tcggtcaccg ggtcggccgt cgggtccggt gcggtgtcgg tgggcgcacc
     2221 gacggcgtcc ggcaccgttc cggccccgac cgcgggtacg gcgaacccgg cggccaccgc
     2281 gacggccgat ccggtgaagg tccttctcct catgtcactg cctcctgtgc tcgtcgacat
     2341 cccgggcgcc ggatgcgtac gggaagcggt cccgacggcg gccggtcccg acctcgccct
     2401 cagtgaacgc aggcgcggaa cgttacgtca actgattctg caatttttcc cacgtagccg
     2461 tcggaacttt cattggcgag gacgggctaa gctaccggcc atggacgtcg tgacccacgt
     2521 gttcgtggcc ctccacctgc tcagcatggc cgccatcgtc ggcctctggt tcgcccactt
     2581 ccgcaccccg acggtgaccc gcggccagct cgtcggcgcc tgcggcagcc tggtgaccgg
     2641 gctggcgctc accggcttcg cggagatggc cgcgaccgcc gacgatccgg tcaaccatgc
     2701 caagatcggg gtgaaggccg tgctggccgt ggtggtgctg gtggccgcga tcatcggggt
     2761 gcgcaaggcc cgcacgggtg gccaggtgcc gacgggtctg gcccatgccg tcggtggcac
     2821 cgcggtgatc aacgtcctga tcgccaccct gtggatgtga ccacgggtac cgtgccccgc
     2881 ggagacgacc agatgcacgg acgaggcaag gaggcccgat gaccatccac cggctactgg
     2941 tggcctgcac cgacggggtg cgccaactgc acctcgacac cacgaccggc acggccgaga
     3001 cggtcggggc ggtgctcccg tacggcacct cgctggcggt caccgcccac cccgccacgc
     3061 cgggtgccgg ctggatcgcc tcggccgacg acggcgggca gctggtcgcg gtcaccgcca
     3121 ccgacgacgg ggtgcgggtc gagaccagca ccggctcgaa cggtgcggtc ccctgccatg
     3181 tcgccgtccc ggacggtcag gtcgcggtcg ccaactacag cggccatacg gtcgcggtgt
     3241 tcccgtacca ccacgactcc gggcaccgga cggccgggcc cgccgtggtg caccacttcg
     3301 gtgagcagtc ccatccgcac caggtgctgg aggtcgacgg ccagctcgtc gtcagcgacc
     3361 tcggtcgcga cggcctgcac gtgctcgccc ccgatgccgc cccggccccg gcggacgggc
     3421 ccccggcgat cccgctggag ccgggagccg gcccgcgcaa cgcggtacgg gtcgggcccg
     3481 accacctggt ggtggccctg gagcgggcga acgcggccgc ggtggtggag ctgcagcgcg
     3541 acgccgccgg caccgtcgtc ggtggccgcc aggtcgaccg gtgcggcttc gacggggaga
     3601 ccagcgccac ccaccccagc cagatcctgc tcgccggggt cgagggagac accgcgcagg
     3661 tgctggtgct gaaccgcggc agcgaccggt tgtgcgtgct ggaggtcacc gacggccggt
     3721 tcggcggcaa ggccgtcgag cacccgctgc ccgcctggcc gatggacctg ctgcggctgg
     3781 acgacgtgct gctggtggcg tgccgggacg ccgacctgct ggtcggcctg gacgccgccg
     3841 acctcgggtc ggacggtgcg gcgcgcacgc tgttctcggt gccggtgccc cagccgaacg
     3901 gggtggcgag cgtcgggaac gtcgcgtgag cgcactcggc gaccggctgc gcagcctgcc
     3961 gacgttcccg gcggacctgc cgacgctgga tccgtcggcg gtcccggacg atccgaccgc
     4021 attgctgctg gcctggctcg acgaggcgat cgccaccggt gagcggcagg ccaatgcgat
     4081 gacgctgacc acggtcgacg acgagggacg gccggcgaac cggacgttga tcgtgaagga
     4141 cgtcgacgac cgcggcctgc acttctccag ctcactcggc tcccggaagg ccgcgcacct
     4201 ggcggtccgc ccgtacgccg ggatgatgtt cttctggcgg ccactggggc gccagctcga
     4261 ggtgtccggc ccggtggtgg ccctggacgc cgacaccagc gccgcggact ggcggcagcg
     4321 gcccagctac gacggccgcg acaacccgga ctggcaggtg tgggcgctgc agccggcgcg
     4381 gtacgagttc ctgcaggcga cgctggaccg ccgccacgtc cgggtggagt acgtccgcac
     4441 cggggacggc tggcggcaca cccggttgct gccgagcgaa cagccgcgct gaccccgggg
     4501 gctcgcgagg tcagccggcc agctgggaga cggcggcgac gtgggcggcc atgtcgcggg
     4561 catcaccgat ggcgttgacc acagaccagc gcaccacgcc ctcggcgtcc agcacgaagg
     4621 agccccgcag cgcacagccg gactcctcgt cgaacacccc gaacgcccgg gcgatggccc
     4681 cgtgcggcca gtggtcggtg ac
//
LOCUS       DATGUK010000048         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1339479,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000048 DATGUK010000000
VERSION     DATGUK010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339479"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..2024
                     /locus_tag="VK103_00735"
     CDS             60..2024
                     /locus_tag="VK103_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLQ82032.1"
                     /translation="MADLPTDDLGTVPLPAVTDLLPPADAELVVVTDTHLVDTDTPHA
                     GEFDSRLRQNERIATALRVIAGTGLPRLVHLGDLVQDYPENPTHQGLLALAARQVRAA
                     AGAVWFAPGNTDIGDKPDPSSPAAPADPRSLERWRAETGGGWTSFRHDDVHGIVLAAS
                     VFNSGLPAEDEQWRWLESELADSPARWRLLFWHHPTYWRDRDDPGLGNYDVIAEPART
                     RLLDLATSHDVTGVFTGHSHFAFLNRVQDLRLYGVPSTSFTRPGFSELFSGPAPAGHG
                     RDDVAKLGLHLVRLHGSGPTRLRVHQVRTGLLTERLKRTPGARPVLTATPADLPGAAL
                     GVVARHPISREGEVPDVFPSVRRSPVRDDYPLLACLDLGVGQLSVTPDDLADPSRAER
                     IALLRAEGVSVAVRMLWPAGAISSYEFVPPRFAGLVGRVDEVELVLLDRAVPTGADRD
                     WLRHVVDSLAPVRLATLMRTARGAAELPRWRYGFTAAEATELGSVAGVVRLLVCSSDR
                     PSLPTGIGPVDLVHALRAGEVLADVVEEFVEARRAGHRFLVDGLVELDRTLDVRPGLL
                     DRIGNPTPYHHALAVLNSALHGPVRISRDGDRYRVVRADGRRLVVDLRREPDGGAARP
                     AGAADEVVDLSDAMTCRPSECAGPWLELSA"
     gene            2143..2277
                     /locus_tag="VK103_00740"
     CDS             2143..2277
                     /locus_tag="VK103_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82033.1"
                     /translation="MINILAAVRSEIGSLGLVSHVVRIAGYVAPSAVLLVRVPMHNRV
                     "
     gene            2274..>2912
                     /locus_tag="VK103_00745"
     CDS             2274..>2912
                     /locus_tag="VK103_00745"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLQ82034.1"
                     /translation="MTDLAERVPTDLAGSTSLVDGIRLALRSAIAQGSLPPGYRLREI
                     PLAAHFECSTTPVREAIRKLEAEGLVRIYPRRGAEVTAFSISEVEHLYETRLVLETHA
                     ARKAARRRPTEEELAPVREVLARHKAAIDEGQPGPPVDDEFHEALTALAGNPVIAELA
                     ARLTRQIVSVQARTGTQVQGGRTAAYAAHTEILDAVAAGDARAAERLMREHLE"
BASE COUNT          380 a         1089 c         1028 g          415 t
ORIGIN      
        1 acgggtcacg tccctcccga ccgcctccgc ctccgccacc gccgtcacca ccgccgccga
       61 tggccgacct cccgaccgac gacctcggca cggtaccgct gcccgccgtc accgatctgc
      121 tgccaccggc cgacgccgag ctcgtcgtcg tcaccgacac ccacctggtc gacaccgaca
      181 ccccgcacgc cggtgagttc gactcccggc tgcgtcagaa cgaacgcatc gcgaccgcgc
      241 tccgcgtgat cgccggcacc gggctgcccc ggttggtgca cctcggtgac ctggtccagg
      301 actacccgga gaaccccacc caccagggac tgctcgccct cgcggcacgg caggtgcggg
      361 cggcggccgg tgccgtgtgg ttcgcccccg gcaacaccga catcggcgac aagccggacc
      421 cgtccagccc ggccgcgccc gccgaccccc gatcgctgga gcggtggcgg gccgagaccg
      481 gtggcggctg gacctccttc cgccacgacg acgtgcacgg catcgtgctg gccgcgtcgg
      541 tgttcaactc cggcctgccc gccgaggacg agcagtggcg ctggctggag tccgagctgg
      601 ccgactcacc cgcgcggtgg cgcttgctgt tctggcacca cccgacctac tggcgcgacc
      661 gggacgaccc cggactgggc aactacgacg tgatcgccga gcccgcccgc acccgactgc
      721 tggacctggc caccagccac gacgtcaccg gggtgttcac cgggcactcg cacttcgcgt
      781 tcctcaaccg ggtgcaggac ctgcggttgt acggggtccc gtccacgtcg ttcacccgcc
      841 cggggttcag cgagctgttc tccgggcccg ccccggccgg tcacggccgg gacgacgtcg
      901 ccaagttggg tctgcacctg gtccggctgc acggatcagg tcccactcgg ctgcgggtcc
      961 accaggtccg gaccgggctc ctcaccgaac ggttgaagcg cacaccgggg gcccgcccgg
     1021 tgctcaccgc gacaccggcg gacctgcccg gagccgcgct cggtgtggtc gcccgccacc
     1081 cgatctcccg cgagggggag gtgcctgacg tgttcccgtc ggtgcggcgt tccccggtcc
     1141 gggatgacta cccgctgttg gcctgtctgg acctcggtgt cggacagctc agcgtgacac
     1201 cggacgacct cgccgacccg tcccgggccg agcggatcgc cctgctgcgc gccgaggggg
     1261 tctcggtggc ggtccggatg ctgtggccgg ccggtgccat ttcttcatat gaattcgtgc
     1321 cgccacggtt cgccggactc gtgggccggg tcgacgaggt ggagctggtg ctgctggacc
     1381 gtgcggtccc gaccggtgcg gaccgggact ggctacggca tgtggtcgac tcgctcgcgc
     1441 cggtccggct ggccaccctg atgcgtacgg cccggggcgc ggccgaactg ccccgctggc
     1501 ggtacggctt cacggccgcc gaggccaccg agctcgggtc agtcgccggg gtcgtccggc
     1561 tgctggtgtg ctcgtccgat cgcccgtcat tgcccaccgg gatcgggccg gtcgacctgg
     1621 tccatgcgct gcgggccggt gaggtcctgg ccgacgtggt ggaggagttc gtggaggccc
     1681 gccgcgcggg acatcgcttc ctggtcgacg gtctggtcga actggaccgc accctggacg
     1741 tccggccggg gctgctggac cggatcggca acccgacgcc gtaccaccac gccctggcgg
     1801 tgctgaactc cgcactgcac ggtccggtcc ggatcagccg ggatggtgac cggtaccgcg
     1861 tcgtacgggc ggacggcaga cggctggtgg tcgacctgcg tagggaaccc gacggcggag
     1921 cggcccggcc ggccggggcc gcggacgagg tcgtggacct gtcggatgcg atgacgtgtc
     1981 gaccgtccga gtgtgccggc ccctggctgg aactgtcggc ctgagcagga tcgggaccac
     2041 ccatgacgcg atccgaccgg cccaccaacg atccggcgga cctgggcctg ctgggcgcgg
     2101 acccgggacg tggcgaccgg acagcagtcg gcacggggcg ccatgatcaa catcctggcg
     2161 gcggtgcgca gcgagatcgg cagcctcggc ctggtcagcc acgtcgtgcg gatcgccggg
     2221 tacgtcgcgc cttcggctgt cctcctcgtg cgcgtgccga tgcataatcg ggtatgaccg
     2281 atttggccga gcgcgtcccc actgatctcg ccggatccac cagtctcgtc gacgggatcc
     2341 gcctcgcgct gcgatccgcg atcgcccagg gttcactgcc acccgggtac cgactgcgtg
     2401 agatcccgtt ggccgcccac ttcgagtgct ccaccacgcc ggtgcgcgag gcgatccgca
     2461 aactggaggc cgagggtctg gtccggatct acccgcgccg cggggcggag gtgaccgcct
     2521 tctcgatctc cgaggtcgaa cacctctacg agacccgcct ggtgttggag acccatgccg
     2581 cccgcaaggc ggcccgccgc aggccgaccg aggaggagct cgccccggtc cgcgaggtgc
     2641 tcgcgcgcca caaggccgcg atcgacgagg ggcagcccgg ccccccggtg gacgacgagt
     2701 tccacgaggc gctcacggcg ctggccggca atccggtgat cgccgagctg gccgcccggc
     2761 tcacccgcca gatcgtctcg gtgcaggcac ggaccggcac ccaggtgcag ggcgggcgta
     2821 cggcggccta cgccgcgcac accgagatcc tcgacgcggt ggccgcggga gacgcccggg
     2881 ccgcggaacg gctgatgcgt gagcacctgg ag
//
LOCUS       DATGUK010000049         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_986500,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000049 DATGUK010000000
VERSION     DATGUK010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986500"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VK103_00750"
     CDS             complement(<1..356)
                     /locus_tag="VK103_00750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HLQ82035.1"
                     /translation="MSTKLLTRTRERITTAVRNRVNGEDFARTPPVWQTPGERWFAPA
                     DAIWRVHRDTAMFIGGIRALLMQSLHPVAMQAVSEHSGYRSDPWGRLQRTSQFISVTT
                     YGTVGEAESAIGKVDH"
     gene            complement(418..2082)
                     /locus_tag="VK103_00755"
     CDS             complement(418..2082)
                     /locus_tag="VK103_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLQ82036.1"
                     /translation="MVIGDRNERLTPGVSRRAVLRGGLAGAAGLAGISGLAACGPEGG
                     GAGGAGGAGDGSLELPTYVPFEDVEPDLPGTAEGVPPGFYNYPDPPIEREGFPLQGVD
                     PFTFLVTGTPPAVLPEDNKNYAWMDEQLGTPSTTIFGTYSDYTSKFQVTMASGDLPDL
                     AIVIQVPGLPQLLEKNFTDLTDVLGGDGVKKYPGLANIPNSAWQLCTVNGRLWGVTRP
                     QPPTGRVINYRSDVIEKRGLTEADLAPQSGDEFLELMKALTDKDNNLFAMGADPTSWL
                     MSISKQMAGTPNGWAEENGTFVSDIASEQMKDALDYAKQVWAAGVMHPNSFSDPSQNG
                     TWYDAGVTALYVRGFGGWDFNIRAFPEATIGALIPPKLEGGGPAPVHKSDAGYPAFTA
                     IRKTDDADRIDQFLRVFDFFASPFGTDQYLGFNYGVEDYAYTMEGPNPVPIDDAPETP
                     IYHTYAGGARNSVIYGQGDRTSVDLRHAYAMEVLPTGVDDASTGLYSETEVTKGTTMN
                     KQMHDVMRQIIQGQQGMSAWDDFVKMWKSRVGDQVAEEYAEAAAAR"
BASE COUNT          304 a          712 c          719 g          392 t
ORIGIN      
        1 atgtggtcga ccttgccgat cgcagactcg gcctcaccga cggtgccgta cgtcgtcacg
       61 ctgatgaact ggctggtccg ctgcagccgt ccccacgggt cgctgcggta gccggagtgc
      121 tccgagaccg cctgcatcgc gaccggatgc agggactgca tcagcagcgc gcggatgccg
      181 ccgatgaaca tcgcggtgtc gcggtgcacc cgccagatcg catcggccgg cgcgaaccac
      241 cgttcgccgg gggtctgcca gacgggcggg gtgcgggcga agtcctcgcc gttcacccgg
      301 ttgcgcaccg cggtggtgat ccgctcccgg gtccgggtca gcagcttggt ggacacgccg
      361 caatgctacc cggggccgcc ggcatccgcg acgcggccgg cgccggccag acgatcatca
      421 ccgtgcggct gccgcctccg cgtactcctc ggccacctgg tcgccgaccc gggacttcca
      481 catcttcacg aagtcgtccc aggccgacat gccctgctgg ccctggatga tctggcgcat
      541 cacgtcatgc atctgcttgt tcattgtggt gcccttcgtg acctcggtct ccgagtacag
      601 tccggtgctc gcgtcgtcga caccggtcgg cagcacctcc atggcgtagg catgacgcag
      661 gtcgaccgat gtgcggtcgc cctggccgta gatcaccgag ttgcgtgccc ccccggcata
      721 ggtgtggtag atcggtgtct ccggggcgtc atcgatcggc accgggttgg gcccttccat
      781 cgtgtaggcg taatcctcga cgccgtagtt gaagccgagg tactggtcgg tgccgaacgg
      841 tgaggcgaag aagtcgaaga cccgcaaaaa ttggtcgatc cggtcggcgt cgtcggtctt
      901 gcggatcgcg gtgaacgcgg ggtagccggc gtctgacttg tgcaccgggg cggggccgcc
      961 gccctccagc ttcggcggga tcaacgctcc gatcgtggcc tcggggaagg cacggatgtt
     1021 gaagtcccag ccaccgaagc cccgcacgta cagtgccgtc accccggcgt cgtaccaggt
     1081 gccgttctgg ctggggtccg agaacgagtt cggatgcatc accccggcag cccacacctg
     1141 cttggcgtag tcgagggcgt ccttcatctg ttccgaggcg atgtcgctga cgaaggtgcc
     1201 gttctcctcg gcccacccgt tcggggtgcc cgccatctgc ttcgagatcg acatcagcca
     1261 cgaggtcggg tcggcgccca tcgcgaacag gttgttgtcc ttgtcggtca acgccttcat
     1321 cagctcgagg aactcgtcgc cactctgcgg agccaggtcc gcctcggtca ggccgcgctt
     1381 ctcgatcacg tcactgcggt agttgatcac ccggcccgtc ggcggctggg gacgggtgac
     1441 gccccagagc ctgccgttga cggtgcacag ttgccacgct gagttgggga tgttcgccag
     1501 gcccgggtac ttcttcactc cgtcaccacc gaggacgtcg gtgaggtcgg tgaagttctt
     1561 ctccaacagc tgtggcagtc cgggcacctg gatcactatg gccaggtcgg gcaggtcgcc
     1621 gctggccatg gtcacctgga acttgctcgt gtagtcgctg taggtgccga agatcgtcgt
     1681 cgacggcgtc ccgagctgct cgtccatcca cgcgtagttc ttgttgtcct ccgggaggac
     1741 ggccggtggg gtaccggtga ccaggaaggt gaacgggtcg acgccctgca gcgggaatcc
     1801 ctcccgttcg atgggcgggt ccgggtagtt gtagaagccc gggggcaccc cctcagcggt
     1861 gccgggcagg tcgggctcga cgtcctcgaa cgggacgtac gtggggagtt cgagtgatcc
     1921 gtccccagcg ccgcctgctc cgcctgcccc gccaccttcg gggccgcagg cggcgagccc
     1981 ggagatcccg gccagtccgg cagcacccgc gagtccacca cgcaacaccg cgcgtcggga
     2041 gaccccgggg gtcagtcgtt cgttccggtc accgatcacc atgggagcgc tcctcagtcg
     2101 tgttgatcat gctggcgttc gtgtgtg
//
LOCUS       DATGUK010000050         3925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_760684,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000050 DATGUK010000000
VERSION     DATGUK010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760684"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VK103_00760"
     CDS             <1..283
                     /locus_tag="VK103_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82037.1"
                     /translation="TGIGAVVTIVLCAAAPPAPDTGPADDRPDAQERPEPEVDLTALQ
                     GAARATVLKDRRKALTNLADRGLLEGHDIDELMARPLGQLVDPDAGRTD"
     gene            399..1376
                     /locus_tag="VK103_00765"
     CDS             399..1376
                     /locus_tag="VK103_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HLQ82038.1"
                     /translation="MMRSHAMTSLRRRIGAVLTATAVAAGLALLGNPTPAEAASPHQL
                     RVITHNIAGGPDFRGSPMALYGVNTEIKKFSPDVVMLTEVCESQVRAFRKAHPSWHFR
                     FTVMIDNQRRCEEADGATGQRQGQLLASPHPMTGRYEHELGYPDHGKPGDNPRRTKRF
                     SLLCSDIAIPGHSATGLRACVTHLRAFNSEEAHRAREAQTKRIREALHDVIWKQGKAV
                     TVGGDFNAKPNWNAMDDLYRLNRDSSYTGRGDFHEADQTDPKYRKDHGSVTCGPKACR
                     NGQPTKDTRKLDYVFISRNVTHRGRVSGLATDRYNSDHKLYRALFEIRY"
     gene            complement(1388..2614)
                     /locus_tag="VK103_00770"
     CDS             complement(1388..2614)
                     /locus_tag="VK103_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82039.1"
                     /translation="MTITEYDVGMATVDITPPVGGPLGGFAARERHTSTSVYHPLRAV
                     ATAIGDGEHDVMIISLEWLGCYQLADRIRRAVTRTSGIPEDAVVVNASHTHCGPAVHG
                     TGLVAHGWIDDDYVDSAIAAIARAAGIAARHRNRCTIRYGIGRCDLAMNRRRPDPDRP
                     PRVFDAMLPNPDGPVDHDVPVLTCHDAASGAVRGILFSYACHPTSRGGLAIGGDYVGF
                     AYDHLAEAFPDAQLGFLQGCGGDAKPRPADPGADTRQVAGPIAVRSAIEVLRTEPFTP
                     EGLVQAAAAPEGFRRRWAEHWQATLDRGDPVETTVPFEIRTVTIGRSLALVGLAGEMT
                     AEHGLRLKRDHGDGYDAVLPLGYTGAMVGYVPVQRQFDEYGYEVELANLWHLRTGRYL
                     PDTEDRVHARIARLLG"
     gene            2961..3092
                     /locus_tag="VK103_00775"
     CDS             2961..3092
                     /locus_tag="VK103_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82040.1"
                     /translation="MYLAPMPAAWQPIESERSKSQRAYRRWLEMQAATPVRRSRRRG"
     gene            3540..3716
                     /locus_tag="VK103_00780"
     CDS             3540..3716
                     /locus_tag="VK103_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82041.1"
                     /translation="MYSQHLPLAWHPRESELSKSRRAHRRWRQAEALRSLARRRRRRQ
                     SVVGQPGRRPALAP"
     gene            complement(3738..>3925)
                     /locus_tag="VK103_00785"
     CDS             complement(3738..>3925)
                     /locus_tag="VK103_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HLQ82042.1"
                     /translation="IPVPAFSASLAYFDSLRRGRLPAALVQGQRDLFGAHTYQRVDVD
                     GSWHIEWSGDGEEVSAD"
BASE COUNT          622 a         1427 c         1330 g          546 t
ORIGIN      
        1 caccggcatc ggcgcggtcg tcacgatcgt gctctgtgcg gccgctcccc cggcaccgga
       61 caccgggccg gccgatgacc ggccggatgc gcaggagcgc ccggagccgg aggtcgacct
      121 gaccgccctg cagggcgcgg cccgtgccac tgtgctgaag gaccggcgca aggccctgac
      181 gaacctggcc gaccgcggcc tcctcgaggg ccacgacatc gacgagctga tggcgcgacc
      241 gctcggacaa ctggtcgatc ccgacgccgg gcgcaccgac tgagccacca cggaaatggg
      301 ggcattgccc atcgcgtact gcgcgcgcac ccagtctcgc ccccggcgtg cctaccgtga
      361 aatggccgat caccttcgat ccccgaggac gaggagccat gatgcgtagt cacgccatga
      421 ccagtctgcg gcgccggatc ggcgccgtcc tcaccgccac cgccgtcgcg gccggcctgg
      481 ctttgctggg caaccccacg ccggcggagg cggccagccc acatcagctg cgggtcatca
      541 cccacaacat cgccggcggc ccggacttcc gcggctcgcc gatggccctg tacggggtga
      601 acaccgagat caagaagttc tcccccgacg tggtgatgct caccgaggtg tgcgagagcc
      661 aggtccgcgc cttccgcaag gcccatccca gctggcactt ccggttcacg gtgatgatcg
      721 acaaccagcg caggtgcgag gaggccgacg gggccaccgg gcagcgacag ggccagctgc
      781 tggcctcccc gcacccgatg acaggtcgct acgagcacga gctggggtac ccggaccatg
      841 gcaagccggg cgacaatccg cgccgcacca agcggttcag cctgctgtgt tccgacatcg
      901 ccatcccggg ccactcggcg accgggctgc gtgcctgcgt cacccacctg cgcgccttca
      961 actccgagga ggcccaccgg gcccgcgagg cgcagacgaa gcggatccgc gaagcgctcc
     1021 atgacgtgat ctggaagcag ggcaaggcgg tcacggtggg cggcgacttc aacgccaagc
     1081 cgaactggaa cgcgatggat gacctgtacc ggttgaaccg cgacagcagc tacaccggcc
     1141 gcggcgactt ccacgaggcg gaccagaccg atccgaagta ccgcaaggac cacggcagcg
     1201 tcacctgcgg cccgaaggcc tgtcgcaacg ggcagcccac caaggacacc cggaagctgg
     1261 actacgtgtt catctcccgc aacgtcaccc atcgcggccg ggtcagtggt ctggcgaccg
     1321 acaggtacaa ctccgaccac aagctgtacc gggcgctgtt cgagatccgg tactgagcga
     1381 cgcgtggtca gccgagcagc cgcgcgatgc gggcgtggac gcggtcctcg gtgtcgggca
     1441 ggtagcgccc ggtgcgcaag tgccagaggt tggccagctc gacctcgtag ccgtactcgt
     1501 cgaactgccg ctgcacgggc acgtagccga ccatcgcgcc ggtgtagccc agtggcagca
     1561 ccgcgtcgta gccgtcgccg tggtcgcgct tcaaccgcag cccgtgctcg gcggtcatct
     1621 cgccggccag tccgaccagg gccagcgaac ggccgatggt gacggtccgg atctcgaacg
     1681 ggaccgtggt ctccaccggg tcgccacggt ccagggtcgc ctgccagtgc tcggcccagc
     1741 gccgccggaa cccctccggc gccgccgcag cctgcaccag cccctccggg gtgaacggct
     1801 cggtccgcag cacctcgatg gccgaccgta cggcgatggg cccggccacc tgccgggtgt
     1861 cggcccccgg atcggccggg cgtggtttgg cgtcaccgcc gcagccctgc aggaacccca
     1921 gctgcgcatc ggggaacgcc tcggccaggt ggtcgtaggc gaagccgacg tagtcacccc
     1981 cgatggccag cccaccgcgg gaggtcggat ggcaggcata gctgaacagg atcccccgca
     2041 ccgcaccgct ggccgcgtca tggcacgtca gcaccggcac gtcgtggtcg accgggccgt
     2101 ccgggttcgg cagcatcgcg tcgaacaccc gcggtggacg gtccgggtcc gggcggcgac
     2161 ggttcatcgc caggtcgcag cgcccgatgc cgtaccggat ggtgcagcgg ttgcgatgtc
     2221 gggccgcgat gccggcggcg cgagcgatcg cggcgatggc ggagtcgacg tagtcgtcgt
     2281 cgatccagcc gtgcgcgacc aggccggtgc cgtgcacggc gggaccgcag tgggtgtggc
     2341 tcgcgttcac caccacggcg tcctcgggga tcccgctggt acgggtgacc gcgcgccgga
     2401 tccggtcggc gagctggtag cagcccagcc actccaggct gatgatcatc acgtcgtgct
     2461 cgccgtcgcc gatggcggtc gccaccgccc gcagcgggtg gtagaccgac gtgctggtgt
     2521 gtcgctcccg ggcggcgaac ccgccgagcg gaccgcccac cggtggcgtg atgtccacgg
     2581 tggccatgcc gacgtcgtac tcggtgatcg tcatcgacct gctcctctgc tcgtacgctg
     2641 cttcctgtct agtcgagcaa gatcaaccgt ggcccgaccg gtgacggtgt cccacgtggt
     2701 gagcaggatc accccgtacg gagtcgcgat tcgtgggctg cttgatctat cattgacgtg
     2761 tcgatgacga tgcccggcgc cggtcagacc agatggcgta gcgtccccga atcggagttt
     2821 ccctcccccg ggtcgacgcc catcgaaatc gcagccctgc cgggcaaccc atgacacatt
     2881 ctgcgcgcag tggcgcgacc acccagagga atccgggcca tgcatgtggg tatgcgggcc
     2941 cacagcatcg aggagaacag atgtacctgg cacccatgcc cgcggcgtgg cagccgatcg
     3001 agtccgagcg ctccaagagc cagcgcgcct accgacgctg gctggagatg caggcggcca
     3061 ccccggtccg ccgcagccgc cgccgcggct gagcacgaac ccttcgcacg gagcaccgcg
     3121 ggacctgccc ggggtgctcc gttctcgttg tccgggcctg cggcgattta atccgttgcc
     3181 gcccggcaca ccacacgtct actgttcttg gtgcacggcg atccgtcaga tcgcccgacc
     3241 ggaaggaggt ggcccaatca tggtgatttc attcaccgac acgtacggcg cccttccggt
     3301 cgtcgccggc tgagcgacga ccggggcgcc gtacggcgtc cggccgcttg ccgttgagaa
     3361 cccacacgtc cttgatcgct cgtccctgcg ggcaccgtgt ccggggtcgg catgcaggcg
     3421 tccccaccac acagccagac cctcaccact caggcccggc tcggtcctga ggcgctgcac
     3481 cacgccctga cgtggtccgg cgctgccccg agccggaccg ccacgacagg aagttgatca
     3541 tgtactcgca acacctgcca ctcgcgtggc acccccggga gtccgagctc agcaagagcc
     3601 ggcgtgcgca ccggcggtgg cggcaggcgg aggcactgcg gtccctcgcc cgccgccgtc
     3661 gacgccgaca gtcggtcgtc ggtcagccgg gccgtcggcc cgcactggcc ccgtgaccgg
     3721 cagcagcggc ggggaggtca gtcggcactg acctcctcgc cgtcaccgga ccactcgatg
     3781 tgccaggaac cgtcgacgtc gacacgctgg taggtgtggg cgccgaacag gtcacgctgg
     3841 ccctgcacca gggctgctgg cagccgaccg cgacgcagcg agtcgaagta ggccagcgat
     3901 gcgctgaacg ccggcaccgg gatgc
//
LOCUS       DATGUK010000051         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_987492,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000051 DATGUK010000000
VERSION     DATGUK010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987492"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1363)
                     /locus_tag="VK103_00790"
     CDS             complement(23..1363)
                     /locus_tag="VK103_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HLQ82043.1"
                     /translation="MDNSARTVTTRRFDTPIGEVDALVDGDVARAYGIRYARAGRFEA
                     PVTEPVAREPIEARRPAPACIQVVDPPPGVLRVDGLAGLDQSEDCLRVTVTTPADVAD
                     DEGLPVVVWIHGGAYRNGAADSGLYDPAALVREHRVVVVSVGYRLGVLGYLSGESRPA
                     NLGLLDQREALGWVQRAIVGFGGDPECVTVMGESAGADAIAHLMVSDGVLDTDRRLFR
                     RAILQSAPLGTTRGRRRMRDLQATVLAEIDDAGALPDLTAWDARVAASGRAAGLAGMM
                     PYGVQYDEQPLPSVDEADRRWREVAPYVDLLAGTNEREVALYRDVPPFPSLERIPALG
                     PAVIEILVRTLTAAVFTRPTAAFVRRHQRSGGRARRYVFHGGVRGNPLRGAHAAELPY
                     LFSGSAWSPSSLLDGTSPERVDQLGKGMRAMWAHFARTGVADDGPTRALLRLVG"
     gene            1391..1981
                     /locus_tag="VK103_00795"
     CDS             1391..1981
                     /locus_tag="VK103_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HLQ82044.1"
                     /translation="MRWRRTASAADDPVSAAMAAQDRKRFLPADQQQHADEDRALPIG
                     HHATNSQPSTVHAMITLLDPRPGDRVLDVGAGSGWTVAILGDLVGPGGRVLGVELVGD
                     LVVRARAALADGDQPWVSVTVAHPGRLGAPVQGPWDRILVSADGGRVPGDLTEQLALG
                     GRLVLPVRGRMTVVDRTEDGLTEHRVRGSWQFVPLR"
     gene            complement(1999..2862)
                     /locus_tag="VK103_00800"
     CDS             complement(1999..2862)
                     /locus_tag="VK103_00800"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLQ82045.1"
                     /translation="MRATRLTDRVTHHGEGPVWSPGWRGVRWVDMMAGAIGELDADGT
                     VHRREVGDKVAAVVRPRTRGGWLVAREHDVAVTESVALDAPLRPLVSVLDDDAVRLNE
                     GGCSPAGDFYIGSMGYGAPVGAGDLSRIRPDGTVSVVVPEVTISNGLDWSPDGSRAYY
                     VDTRTDRIDVFDSDPEHGLVNRRPFVEITDTPGHPDGLCVDSAGGVWVALFGGSAVRH
                     YDADGTLDQVIEVDARQVTAVTTGGPDLTTLFITTSREGLTDDRDDPAAGSLFTAEAG
                     TPGQPVRAFAG"
     gene            2910..>3152
                     /locus_tag="VK103_00805"
     CDS             2910..>3152
                     /locus_tag="VK103_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HLQ82046.1"
                     /translation="MTQSQDAPASTLLVIAAGGAAGASLRHLVAVWVPSTPAGWPWAT
                     WLVNLLGALLMGVLMGLLATRPATAGTATALLRPFLG"
BASE COUNT          440 a         1186 c         1099 g          427 t
ORIGIN      
        1 ccagtcgaca gaccggcccg gctcagccga ccagtcgcaa caacgcgcgg gtcgggccgt
       61 catcggcgac gccagtcctg gcgaagtggg cccacatcgc ccgcattccc ttgcccagtt
      121 ggtccacgcg ctccggcgac gttccgtcca gcaaggaact gggggaccag gccgagccac
      181 tgaacaggta tggcaactct gcggcatggg cgccccgcaa cggatttccg cggacaccgc
      241 cgtggaacac gtaccgccgg gcgcgtccgc cggagcgctg gtgccgtcgc acgaacgcgg
      301 ccgtcggtcg ggtgaacacg gcggctgtca gcgttcgtac gaggatctcg atcacggccg
      361 gtcccagtgc cgggatccgt tccagggaag ggaagggcgg cacgtctcga tacagtgcca
      421 cctcgcgttc gttggtgccg gccagcaggt ccacgtacgg ggcgacctcc cgccaccggc
      481 ggtcggcctc gtccaccgac ggcagcggct gctcgtcgta ctgcaccccg tacggcatca
      541 tgcccgcgag cccggccgcc cggccggacg ccgcgacccg cgcatcccag gcggtcaggt
      601 cgggcagtgc gcccgcatcg tcgatttcgg ccagcaccgt cgcctgcagg tcccgcatcc
      661 gccgccgtcc ccgggtcgtc ccgagcggcg cgctctgcag gatcgcccgt cggaacagcc
      721 gccggtcggt gtccagcaca ccgtcactga ccatcaggtg ggcgatcgca tcggcacccg
      781 ccgactcacc catcaccgtg acgcactcgg ggtcaccgcc gaacccgacg atcgcgcgct
      841 gcacccagcc caacgcctcc cgttggtcga gcagccccag attggcaggt cgtgactcgc
      901 cggacaggta gcccagcacg cccaggcggt agccgacgct gacgaccacg acacggtgct
      961 cccggaccag cgccgccgga tcgtacagcc ccgagtcggc cgcgccgttg cgataggcac
     1021 ccccgtggat ccacaccacg acgggaagcc cctcgtcgtc ggcgacatcg gccggcgtgg
     1081 tgacggtgac ccgcaggcag tcctcggact ggtcgaggcc ggcgagcccg tccacccgca
     1141 gcaccccggg cggcgggtcg accacctgga tacaggccgg ggcaggccga cgggcctcga
     1201 tcggctcccg ggccaccggt tcggtgacgg gggcctcgaa ccgaccggcg cgggcgtacc
     1261 tgatcccgta cgcccgcgcc acgtcaccgt ccaccagcgc atcgacctcg ccgatcggag
     1321 tgtcgaaccg tcgggtcgtc accgtacggg cactgttgtc catgggcccc atggtgccaa
     1381 gctggggatc atgagatggc gcaggacggc ctccgccgcc gacgacccgg tgtcggctgc
     1441 gatggcggcc caggaccgga aacggttcct gccggccgat cagcagcagc atgccgatga
     1501 ggaccgcgcc ctgccgatcg gccaccacgc cacgaactcc cagcccagca cggtgcacgc
     1561 aatgatcacc ctgctcgacc cacgcccggg cgatcgcgtg ctggacgtcg gtgccggatc
     1621 cggctggacg gtcgcgatcc tgggcgacct ggtcgggccc ggtggccgag tgctgggcgt
     1681 cgaactggtc ggggacctgg tggtacgggc acgcgcggcg ctggccgacg gcgaccagcc
     1741 ctgggtgagc gtcacggtcg cacacccggg ccgactcgga gcaccggtgc agggcccgtg
     1801 ggaccgcatc ctggtgtccg ccgacggcgg ccgggtcccc ggggatctca ccgagcagct
     1861 ggccctcggt ggccgactgg tcctcccggt ccggggacgc atgaccgtgg tcgaccgcac
     1921 cgaggacggg ctgaccgagc atcgggtacg gggcagctgg cagttcgtgc cgctgcgctg
     1981 actcgggcga gtgtgtgatc agcccgcgaa cgcccgtacc ggctgtccgg gcgtccccgc
     2041 ctcggcggtg aacagcgacc ctgctgcggg atcgtcccgg tcgtcggtca acccctcccg
     2101 cgaggtggtg atgaacagcg tggtcaggtc cgggccgccg gtcgtcaccg cggtcacctg
     2161 tcgggcatcg acctcgatca cctggtccag ggtgccgtcg gcgtcgtagt gccgcactgc
     2221 gctgccgccg aacagggcga cccacacgcc gccggccgag tccacgcaca gcccgtcggg
     2281 atggccgggg gtgtcggtga tctcgacgaa cgggcgccgg ttgaccaggc cgtgctcggg
     2341 atcggagtcg aacacgtcga tccggtcggt gcgggtgtcg acgtagtacg cgcgggagcc
     2401 gtccggggac cagtccaggc cgttggagat cgtcacctcg gggaccacca ccgacaccgt
     2461 cccgtccggc cggatccggc tcaggtcccc ggccccgacc ggcgccccgt accccatgga
     2521 gccgatgtag aagtcacccg ccgggctgca cccgccctcg ttcagccgta ccgcgtcgtc
     2581 gtcgagcacc gacaccaatg ggcgcaacgg ggcatccagg gcgaccgact ccgtcaccgc
     2641 cacgtcatgc tcgcgggcga ccaaccagcc gcctcgggtg cggggccgca ccaccgcggc
     2701 gaccttgtcg ccgacctccc ggcggtgcac cgtgccgtcg gcgtccagtt cgccgatcgc
     2761 gccggccatc atgtccaccc agcgcacgcc tcgccagccg ggggaccaca ccggcccttc
     2821 gccgtggtgg gtgacgcggt cggtcagtcg ggtggcacgc atgcccccat cgtgccccat
     2881 cgctgcggga ccgtacgtac agtgggcccg tgacgcagtc gcaggacgcc ccggcctcga
     2941 cgctgttggt gatcgctgct ggtggggcgg ccggggcctc gctgcgccac ctggtcgcgg
     3001 tgtgggtgcc cagcacgccg gcgggatggc cgtgggccac ctggctcgtc aacctgctcg
     3061 gcgcactgct gatgggcgtg ctgatgggcc tgctcgccac acggcccgcg actgccggca
     3121 ccgcgacggc actgttgcgg ccgttcctcg gg
//
LOCUS       DATGUK010000052         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_766880,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000052 DATGUK010000000
VERSION     DATGUK010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766880"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..963
                     /locus_tag="VK103_00810"
     CDS             178..963
                     /locus_tag="VK103_00810"
                     /inference="COORDINATES: protein motif:HMM:NF025166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HLQ82047.1"
                     /translation="MNRAVGAGSAVQSDGARVTHLMASAWRPTGIRVAAMSSPASPTA
                     SEPPAGDDRTRLLGLLAYAELSTVEQLAGVACLAPSFDRRVGVLDAAGARMDHHRVLV
                     GQLVDAGADPVTAMEPHQGVVDDFHIRTAPRGWPEALLKAWATEGLASDFRVELGRTL
                     PDVADRADRGDGPTRLADRAPQWLAEVLHNDPTAQGRLALWARRLVGELMTRAQRLAA
                     DDEGLAVLVTGASPGSALVEVHAMIAQLLQRHNERMATAGLPA"
     gene            960..1778
                     /locus_tag="VK103_00815"
     CDS             960..1778
                     /locus_tag="VK103_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLQ82048.1"
                     /translation="MSRLSHEVLEVPRGRLGLWTKGTGPDLVVVHGSGLASRDYAGLL
                     RALDDDFTVHVYDRRGRGSSSDVTIGYGVDTEVSDLAAVMAATGATRVFGHDYGGFVA
                     LQAARSHGWDAVATYDAAVSIDGSAPDWWVDDFQDAVADGDHGRAAALMVKGLDLVPM
                     VSRVPLSVGTRVGRRFAASPFGKDWNSRAGATLAEVGEALSHDGPADEYATISARTTL
                     CVGGASAPWFRRSADAIMPVLPDGRGVVVLPGAGHDAPQRGSRRIVRQLVTALS"
     gene            complement(1762..>2128)
                     /locus_tag="VK103_00820"
     CDS             complement(1762..>2128)
                     /locus_tag="VK103_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82049.1"
                     /translation="VVICFLTVGFQTAVNATAGAVRMPLRRYLPAVTLGGAIWALVYA
                     TVGLAAIHAWLAAAVRWPGTVPITVVVVGAVVALLVWRHRRRRAVSTTTATTIRPTTA
                     TVADDGTPVRAGRPVTTAP"
BASE COUNT          285 a          719 c          848 g          276 t
ORIGIN      
        1 cgatccgaca tccggaccgc tcggggagcg tgacgcatgg gtgcgtaccg ggccgagtct
       61 agcagcgggc accacgccga ccccacaccg gccgcgaccg ggcgtgggga gcggtcaaca
      121 gcggtcccgg ctgggcatgc ccgtaccagt gctgtcgatg acccgccggc gattggcgtg
      181 aaccgggcgg tgggcgccgg gtcggccgtg cagtccgacg gtgcacgtgt cacccacctg
      241 atggcctcgg cgtggcgacc gaccggcatt agagtggcgg ccatgagcag tcctgcctct
      301 ccgacggcat cggagccgcc cgccggggat gaccggaccc gactcctcgg cctgctggcc
      361 tacgcggagc tgagcaccgt cgaacagctg gcgggcgtcg cctgtctggc gcccagcttc
      421 gaccggcggg tgggcgtgct ggacgccgcc ggagcccgga tggaccacca ccgcgtgctg
      481 gtcgggcagt tggtcgatgc cggtgccgac ccggtcacgg cgatggagcc ccaccagggg
      541 gtggtcgacg acttccacat ccggaccgcc ccgcggggct ggccggaggc gttgctgaag
      601 gcgtgggcga ccgagggact ggcgagcgac ttccgagtcg agctgggtcg cacactgccg
      661 gatgtggcgg accgggccga ccgtggggac gggcccaccc ggctggcgga ccgggcgccg
      721 cagtggctgg ccgaggtgct gcacaacgac ccgaccgcgc aggggcggct ggcgttgtgg
      781 gcacgccgcc tggtcggtga gttgatgacc cgggcacaac ggctcgcagc cgacgacgag
      841 gggctggccg tgctcgtcac cggggcctcg ccggggtccg cgctggtgga ggtgcacgcg
      901 atgatcgcgc agctgttgca acggcacaac gagcggatgg cgacggcggg gttgcccgca
      961 tgagccggtt gagccatgag gtgctggagg tgccgcgcgg ccggctggga ctgtggacca
     1021 agggcaccgg gcccgacctg gtcgtggtgc acggcagcgg cctggcctcc cgggactacg
     1081 ccggcctgct gcgggcgctg gacgacgact tcaccgtgca cgtgtacgac cgacgcggcc
     1141 ggggcagctc cagcgacgtc acgatcgggt acggcgtcga caccgaggtc tccgacctgg
     1201 ccgcggtgat ggcggccacc ggggcgactc gggtgttcgg ccacgactac ggcggcttcg
     1261 tggcgctgca ggcggcccgc agccacggct gggacgcggt ggccacctac gacgctgcgg
     1321 tctcgatcga cggcagcgcg ccggactggt gggtggacga cttccaggac gccgtcgccg
     1381 acggcgacca cggccgggcc gccgccctga tggtgaaggg gctggacctg gtcccgatgg
     1441 tcagtcgcgt gccgttgtcg gtcggcaccc gggtgggccg gcggttcgcg gccagcccgt
     1501 tcggcaagga ctggaacagc cgggccggtg ccacgctcgc cgaggtcggc gaggcgctga
     1561 gccatgatgg cccggccgat gagtacgcga cgatctcggc gcgcaccacc ttgtgcgtgg
     1621 gtggggcgtc agcgccctgg ttccggcggt cggccgacgc gatcatgccg gtgctgccgg
     1681 acggtcgggg agtggtggtg ctgcccggcg ccggtcacga cgccccgcag cgtggctcgc
     1741 ggcggatcgt tcggcagttg gtcacggcgc tgtcgtgacc gggcgcccgg cccggaccgg
     1801 ggtgccgtcg tcggcgaccg tggcggtggt gggcctgatg gtggtcgcgg tggtggtgct
     1861 cacggcgcgc cgacggcggt ggcgccacac cagcagcgcg accacggcac cgaccacgac
     1921 gacggtgatc gggaccgtac ccggccaccg gaccgcggcg gccagccagg catggatcgc
     1981 ggccaacccg acggtggcgt agaccaacgc ccagatggca ccgccgagcg tgacggccgg
     2041 caggtaacgg cgcaacggca tccgcacagc tccggcggtg gcgttcacag cggtctggaa
     2101 gccaacggtc aggaagcaga tgaccacc
//
LOCUS       DATGUK010000053         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_63934,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000053 DATGUK010000000
VERSION     DATGUK010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63934"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VK103_00825"
     CDS             <1..1113
                     /locus_tag="VK103_00825"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLQ82050.1"
                     /translation="DFLRELLDYSKVNYIGYSYGTWLGTWYADTYPDRVDRFVLDSNM
                     NWVDDMQANADSDSHSFQRRRDAMLFPWIARHHDSYGLGTTADAVEQRYEEIRAKLVA
                     NTKAGLDTPYGYELDLAVLSPIYTDSGFPEAAEAIVAMGEVAFDEETSATAERRIAAA
                     TDGRALSPSARLADARQQAESGEEVIDLGSIGTVVRCNDTPYQTNPQPYLARADRDTR
                     KYSYIGYFNTVPMCAFWPYEQQPRRIDLDNVPTMLMIQSEGDPATATEGAERSHRLTR
                     KHTVMVGVVDEGQHGIYIGGPSACIEQIGDAFVFGGVLPTTMTMCGTTPLPGDSTVHS
                     LDGPFDKYQRRDRHTKPDRANPMLARARTQANQGMF"
     gene            complement(1118..>3703)
                     /gene="cydC"
                     /locus_tag="VK103_00830"
     CDS             complement(1118..>3703)
                     /gene="cydC"
                     /locus_tag="VK103_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HLQ82051.1"
                     /translation="LFPAMFVLVLAPEAFLPLRQVGVHYHDAADGVAAADEALTIIES
                     ADRDTGGQGTAAGRADTSTVGIGPVDAVRLTGATTDRTGALTAEAHSGELTVVTGPSG
                     AGKTTALQLVMGWLRPTAGTVELICGDERLPLAPERVAGWHRQVAWVPQLPGLVPGTV
                     ADNIAFGGPVTDRDRLRAVLDRCGGSDIALDQPVGDDGTGLSVGQRRRVAVARAMLRV
                     IDGGARWLVLDEPTAGLDAGSEAAVLAGLPPGVGVIMVSHRAAVVARADRVVEITPLL
                     PEQHDGMPAAAATTSAHPAARAGARERPADRSGDDRLAAAGPDRGGLAASVVLGVLAA
                     GSGVGLIGTAGWLLSRAAEHPPVLHLMVAVVAVRFFGIGKGVFRYAERLLGHRVALAE
                     ESRLRIDTYRRLADRLVPVRGRDDLVSRLTQDVGAAVDLTVRVLLPTLVAVVVGLGTV
                     TFVATVSPPAAVALAAGLVVAGIVGPRLGAMLARRSQRQLADLRGELADEVGSVHRLA
                     AELTAHGLGRQRLDRVSARDAALRAVEQRAATATGVASAVQLVGLGAVTVTALLVAAP
                     AVRAGTLAPTMVAVLALLPLALADVVAPLPAAALARTASRAALDRVARIGHGPRVAER
                     THLGDDIDDRVDADDAPPGAGAVTLVDASVGWSADLPVLAGVSLTVSAGERVALVGPS
                     GLGKTTLGLTVAGLLAPLDGELDVGGRVGLLSQDAHLFDTTVRENLRLARPDATDAEL
                     ATVLQRVGLRFALDRRVGEHGSTVSGGEARRLALARLLLRRVDVLVLDEPTEHLDHDT
                     AERLLRDIGQLWPTAAVLVITHDSALVASALGARVVDLTPTAVPGAGDAAGASRDAPA
                     GLVSA"
BASE COUNT          554 a         1440 c         1263 g          446 t
ORIGIN      
        1 gacttcctgc gggagctgct cgactactcc aaggtcaact acatcggcta ctcgtacggg
       61 acctggctcg gcacctggta cgccgacacc taccccgacc gggtggaccg cttcgtgctc
      121 gactccaaca tgaactgggt cgacgacatg caggccaatg ccgacagcga ctcgcactcc
      181 ttccaacggc gtcgcgacgc catgctgttc ccgtggatcg cccgccacca cgacagctac
      241 ggactgggca ccacggccga tgcggtcgag caacgctacg aggagatccg ggccaagttg
      301 gtcgccaaca ccaaggccgg cctcgacacc ccgtacggct atgagctcga cctggccgtg
      361 ctgagtccga tctacaccga ctccggcttc ccggaggccg ccgaggccat cgtggcgatg
      421 ggtgaggtcg cgttcgacga ggagacctct gccactgcgg agcggaggat cgcggccgcc
      481 accgacgggc gcgcactgtc cccctcggcc cggttggccg atgcccggca gcaggccgag
      541 tccggtgagg aggtgatcga tctcggatcg atcggcaccg tggtccgctg caacgacacc
      601 ccgtaccaga ccaacccgca gccgtacctg gcccgggccg atcgcgacac ccgcaagtac
      661 agctacatcg gctacttcaa caccgtgccg atgtgtgcgt tctggccgta cgagcagcag
      721 ccgcgccgca tcgacctgga caacgtcccg acgatgctga tgatccagtc cgagggggat
      781 ccggcgaccg cgacggaggg ggccgagcgc agccaccggc tgacccgcaa gcacaccgtg
      841 atggtcggcg tcgtcgacga gggccagcac ggcatctaca tcggtggccc gtcggcgtgc
      901 atcgagcaga tcggtgacgc gttcgtcttc ggcggggtgc tgccgacgac catgacgatg
      961 tgcggcacca ccccactgcc gggcgacagc acggtccact cgttggacgg tccgttcgac
     1021 aagtaccagc ggcgcgaccg ccacaccaag ccggaccggg cgaacccgat gctggcccgg
     1081 gcccgcaccc aggccaacca gggcatgttc tgaccggtca ggccgacacc aatcctgcgg
     1141 gggcgtcccg cgatgcccct gcggcatctc cggctccggg cacggcggtc ggggtgaggt
     1201 cgaccacccg ggcccccagt gccgacgcca ccagggcgga gtcgtgggtg atcaccagca
     1261 ctgccgccgt cggccacagc tgaccgatgt cgcgcagcag ccgttccgcg gtgtcgtggt
     1321 ccaggtgttc ggtcggctcg tcgagcacca ggacgtccac ccggcgcagc agcagccgcg
     1381 ccagcgccaa ccgccgcgcc tcgccgcccg agaccgtgct gccgtgctcg ccgacccggc
     1441 ggtccagggc gaaccgcagc cccacccgct gcagcaccgt ggccaactcg gcgtcggtgg
     1501 cgtcgggccg ggcgagtcgc aggttctccc gcaccgtggt gtcgaacaga tgggcgtcct
     1561 gggacagcag cccgacccgg ccgccgacgt cgagctcgcc gtccagcggc gccagcagcc
     1621 cggccacggt gagcccgagt gtggtcttgc ccagtccgct gggaccgacc agcgccactc
     1681 gttcaccggc cgacacggtg agcgagaccc cggccagtac ggggaggtcg gcgctccagc
     1741 cgacgctggc gtcgaccagc gtgaccgcac cggcaccggg cggcgcgtcg tccgcatcga
     1801 cccggtcgtc gatgtcatcg ccgagatggg tccgctcggc cacacgtggc ccgtggccga
     1861 tccgcgccac ccggtcgagt gccgcccggg acgccgtccg ggccagtgcg gccgcgggca
     1921 gcggggcgac cacgtcggcc agggccagcg gcagcaacgc caggacggcc accatggtcg
     1981 gcgcgagcgt cccggcccgg accgcggggg ccgccaccag cagggcggtg acggtgaccg
     2041 ctccgaggcc gaccagctgc accgccgagg cgaccccggt ggccgtcgcg gcgcgctgct
     2101 cgaccgcacg cagcgcagca tcccgggccg acacccggtc gaggcgttgc cgcccgagcc
     2161 cgtgcgcggt gagctccgcg gcgagccggt gcaccgaccc gacctcgtcg gcgagctcgc
     2221 cgcggaggtc ggcgagttgt cgctgcgagc gccgggccag catcgcgccc aaccgcggcc
     2281 cgacgatccc ggccaccacc agtccggcgg ccagggccac cgcggccggc ggcgacacgg
     2341 tcgccacgaa cgtcacggtg ccgagcccga ccaccactgc caccagcgtc gggagcagca
     2401 cccgtacggt caggtccacc gccgcaccga cgtcctgggt gagccgactg accaggtcat
     2461 cacgcccgcg gaccggcacc agccgatccg ccagccgccg gtaggtgtcg atgcgcagcc
     2521 gggactcctc tgccagcgcc acccggtggc ccagcagccg ttcggcgtaa cggaacaccc
     2581 ccttgccgat cccgaagaat cgcaccgcca ccacggcgac catcagatgc agcaccggtg
     2641 ggtgctcggc ggctcgggac agcaaccagc cggccgtgcc gatcaggccc accccggagc
     2701 cggccgccag cacgcccagc accaccgacg cggcgagccc acctcggtcc ggtcccgccg
     2761 cagcgagccg gtcgtcaccc gaccggtcgg ccgggcgctc gcgtgccccg gcccgagcgg
     2821 ccgggtgggc cgaggtcgtc gcagccgcgg ccggcatgcc gtcgtgctgc tcgggcagca
     2881 gcggtgtgat ctcgacgacc cggtcggcgc gcgcgaccac ggcggcccgg tggctgacca
     2941 tgatcacccc gacaccgggc ggcagcccgg ccagcaccgc tgcctcgctg ccggcgtcca
     3001 gcccggccgt gggttcgtcg agcaccagcc agcgggcacc gccgtcgatg acccgcagca
     3061 tggcccgggc gaccgcgacc cggcgtcgtt gcccgaccga caggccggtc ccgtcgtcac
     3121 ccacgggctg gtcgagtgcg atgtcgctgc caccgcagcg atccagcacc gcgcgcagcc
     3181 ggtcacggtc ggtgaccgga cctccgaacg cgatgttgtc ggccactgtg ccgggcacca
     3241 gtccgggaag ctgtggcacc caggccacct gccggtgcca ccccgccacc cgttccggtg
     3301 cgagcgggag tcgctcgtca ccgcagatga gttcgaccgt ccccgcggtg ggacgcagcc
     3361 agcccatcac cagctgcaga gcagtggtct tgccagcccc cgacggcccg gtcaccaccg
     3421 tcagctcccc gctgtgggcc tcggcggtca gcgcacccgt acgatccgtg gttgccccgg
     3481 tcagccgcac cgcatcgacc ggaccgatcc cgacggtgct cgtgtccgcc cgtcctgcgg
     3541 cggtgccctg tccaccggtg tcccggtcgg cggactcgat gatcgtcagc gcctcgtcgg
     3601 cggcggcgac cccgtcggcg gcgtcgtggt agtgcacccc gacctggcgc agtggcagga
     3661 acgcctcagg ggccagcacc agcacgaaca tcgccgggaa cag
//
LOCUS       DATGUK010000054         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_296308,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000054 DATGUK010000000
VERSION     DATGUK010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296308"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VK103_00835"
     CDS             <1..978
                     /locus_tag="VK103_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82052.1"
                     /translation="SLDPSSPDFVVTRVNEEVYHPWYRWTATRLEGPADATPQAGTSA
                     LEQRYYAFAVHTGMVTYPMALFTRVVLEERLPRYRGAAQRYRAWTRKAVAHHDHEWQW
                     RELADGSTGGDYVWPKGAPVPFDGLVQPFNQTQGLGLTMSEMHRVDRNPALADRVGAM
                     VRSFRSDMEVDGDAWQWRYWPTYSELFRSYTADEQVSEYTPWYTAATQYEDVSHAAIS
                     VEFMVAAHRAGLGSTEADLGHLVETYRDHVRLDETRVNARVDGTTPAADSIAAQVGRW
                     LGLQPWGSDLAAHVEAVYGAMETEPYGGSHLAGLGHLAWAINEGWRNRG"
     gene            1212..>2036
                     /locus_tag="VK103_00840"
     CDS             1212..>2036
                     /locus_tag="VK103_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLQ82053.1"
                     /translation="MSRRLVLSAPLAVAAASAAGCGFSGPGDSGDGGTGGGGRLTTTL
                     REARRPANHLNPVFKGGGSPQSTILIAMMWEGLVRRDPNDASNYLPGVAESWEASDDG
                     LTHTFVLRDTTWSNGDPVTADDVVWTFSYYFSPELAEQGNENPPRHDGVQSEIAGMAD
                     YYSGKSDDFGDVGVSAPDPHTVVFTLTAPSPFFLDSLVDIVLLHRASVEADVKDFWMP
                     EHYVGNGPYVLTSYSQNANATFELNESYWNAEAFSVTTREVQFNASGATGMMVSYNA"
BASE COUNT          337 a          699 c          699 g          301 t
ORIGIN      
        1 agcctggatc cgagcagtcc cgacttcgtc gtcacgcggg tcaacgagga ggtctaccac
       61 ccgtggtacc gctggactgc cacgcgcctc gagggtcccg ccgatgccac cccgcaggcc
      121 ggcacctcgg cactggagca gcggtactac gcattcgccg tgcacaccgg gatggtgacc
      181 tacccgatgg cactgttcac ccgggtggtc ctggaggagc ggctgccgcg ctaccgcggt
      241 gccgctcagc ggtaccgtgc ctggacccgt aaggccgtgg cacaccacga ccacgagtgg
      301 cagtggcgcg aactggccga cgggagcacc ggtggcgact acgtgtggcc caagggtgcc
      361 ccggtcccgt tcgacgggct cgtccaaccg ttcaaccaga cacaggggct cggcctgacg
      421 atgtccgaga tgcaccgggt ggaccggaac ccggccctgg cggacagggt cggggcgatg
      481 gtgcgcagct tccgctccga catggaggtc gacggcgacg cctggcagtg gcgctactgg
      541 ccgacctaca gcgaactgtt ccgcagctac accgccgacg agcaggtgtc ggagtacacg
      601 ccctggtaca ccgcggccac gcagtacgag gacgtcagtc acgccgcgat cagcgtggag
      661 ttcatggtcg ctgcgcaccg ggcggggctg ggcagcaccg aggccgatct ggggcacctg
      721 gtcgagacct accgcgacca tgtgcggttg gacgagaccc gggtcaacgc ccgggtggac
      781 gggaccaccc ccgctgcgga cagcatcgcg gcccaggtcg gccggtggct cggcctgcag
      841 ccgtgggggt cggacctggc cgcgcatgtg gaggcggtgt acggggcgat ggagaccgag
      901 ccgtacgggg gctcgcactt ggcgggtctc ggccacctgg cgtgggcaat caacgagggt
      961 tggcgcaatc ggggctgacg acgccggggg agcggtgcgg gtcgttggtc cgttccgatc
     1021 acccgccgac ggccgagtgc cgtaaaatga aactcgtgtg ttgactggcg aacctgtccg
     1081 tcctagcctg atcgatcagg cagtcctgga cgcacacacc tcgacgtgga ggtacggtca
     1141 tggctcgatc gacggcacac aacgattccc gcaggatcgc cggccggccc ggcacactgc
     1201 ccgacggact ggtgtcccgc cggctggtgc tgtcggcacc gctggcggtc gcggccgcca
     1261 gtgccgccgg atgcggcttc tccggacccg gtgactccgg tgacggcggt accggcggtg
     1321 gtggccgact gaccaccacc ctccgggagg cccgacgacc ggcgaaccac ctgaacccgg
     1381 tgttcaaggg aggcggctca ccccagtcca cgatcctgat cgcgatgatg tgggagggcc
     1441 tggtccgacg tgacccgaac gacgcctcga actacctgcc cggggtcgcc gagagctggg
     1501 aagcctccga cgacggcctg acccacacct tcgtgctgcg ggacacgacc tggagcaacg
     1561 gtgatccggt gaccgccgac gacgtggtgt ggaccttctc ctactacttc tcaccggagc
     1621 tggccgagca gggcaacgag aacccgccgc gccacgacgg cgtgcagtcc gagatcgccg
     1681 gaatggccga ctactacagt ggcaagtccg acgacttcgg tgacgtcggg gtgtccgccc
     1741 cggacccgca caccgtggtg ttcacgctga ccgcccccag cccgttcttc ctcgacagcc
     1801 tcgtcgacat cgtgctgctg caccgtgcca gcgtggaggc cgacgtcaag gacttctgga
     1861 tgcccgagca ctacgtcggc aacggcccct acgtcctcac cagctattcc cagaacgcca
     1921 atgccacgtt cgagctcaac gagtcgtact ggaacgccga ggcgttctcc gtcaccaccc
     1981 gggaggtcca gttcaacgcc agcggggcga ccgggatgat ggtgtcctac aacgcc
//
LOCUS       DATGUK010000055         6374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1346596,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000055 DATGUK010000000
VERSION     DATGUK010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346596"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="VK103_00845"
     CDS             complement(<1..1482)
                     /locus_tag="VK103_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HLQ82054.1"
                     /translation="MPATDAPAVRHRPPRRLTHWVAATAALLLALTTGTATLPSASAD
                     PSGPSASTAMTPEQAALAARCGTPPRRIPPQVASPVTGYFIFQDTEPCDAMAKMAALH
                     SINADTAITFGSRINKLPGVDEQGRVLLADGTVDPKWVDCVEDGRTCVQAAADAVPDS
                     TIRHHLYYSNTEHLGDAVVRCTDNGRDATVVHNGRMYQRILLPVGEDRGCDAAHDQYD
                     LVLVSGGAADSPNMIDSMLTAADAYGIDFWIGMPVPDKDPESPWLPDRAAFDYTEAFS
                     SRLFTDWQQRYAHYDSWVGVYQSTEIPMKDNAAWDAGYELYTLQHHLAADLMPGRAIM
                     ISPYQDARRDMDVPIEMADDAFIRLAGTAPDGTRIVISPQDSRGTNKMGVYFPDEVDQ
                     IVEEPLWDTVGKVTYSEAYQGNSGDYFASMLEGQRRLDNPDAELWINVELMQRGVGED
                     QETCDDGRSIAAPYERVAKQLSVAQSRVSKVIGYMYDGLMTCTN"
     gene            1701..2903
                     /locus_tag="VK103_00850"
     CDS             1701..2903
                     /locus_tag="VK103_00850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009591457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLQ82055.1"
                     /translation="MHHPSTSNPAAALTRRRLLGAAGATTGAAVTWASARPATAGPRP
                     VDTRPRGGADSPQSSDDSLTWSSPAEAPFRLAGFCWFDQDRVHRRLPVEPDWPLPEAV
                     DRLADNTAGGQIRFRTDSTRVAVRVSLSGPPGMNHMPATGQCGFDAYVGDFGDQQYWG
                     TARPTLDETAYEAVFFTTPEPQTRTVTLNFPLYQGVEEVLVGLDAGASVEAAPDFERP
                     GRLVVYGTSITQGGCASRPGAAYPAELSRRLAIETVNLGFSGSGKGEPEVARTIAQID
                     DVACFVLDYESNVATAGDMRRTLRRFIPILRERHPAVPIVVASKTARIPERYDPDALA
                     DRLAKRKAQADVVARLQRAGDDRLQFLDLGDHDLSASSVDGTHLTDGGFVQLADLIEP
                     SVRRWLSA"
     gene            2950..3600
                     /locus_tag="VK103_00855"
     CDS             2950..3600
                     /locus_tag="VK103_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLQ82056.1"
                     /translation="MKLTHLGHSAVLVEVADRRLLIDPGNFSDAWHGVTDLDAVLVTH
                     LHPDHIDPEHVPALLQANPEAQVRVEPGVISTHHPAGAEPLRAEEELRLGDVTVRAIG
                     GEHAVIHRDIPMIGNIGLVISAPGEPTFFHPGDSLAAVPTGVDVVGVPAYGPWAAMKE
                     TIDFARAVGAPKGFAIHDRLLNDRGFTMLHDRIAEMSPTAMVDLRDGSPYDPGDPA"
     gene            complement(3601..4824)
                     /locus_tag="VK103_00860"
     CDS             complement(3601..4824)
                     /locus_tag="VK103_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLQ82057.1"
                     /translation="MFQPATLPEQYAAAQTDDLRVLVVGAGMAGLTAAQLLRHHGRHP
                     VLVDRARDVDALLDENKAGYMLAMMPMVDPVFDTLGLWHDYLPRGVPLARYGLHDSRG
                     RQVRDDSMSELLSAYGSYQGIARGELIESLASPGCPATFGTTVGALTETDDHTVATLL
                     VDDRRVDLAFDLVVIADGIHSTTRRLVRAGRQVDVLDTGWGGWVVWAPEDADPDLGSE
                     LWGQGFFVGAYPVQGRLGVFLGGPHGRTAIGPAEFATRARRALRTVPPRIDSCLTAVA
                     SAEDPYYWPLADCRAPHWADRRTVLLGDAAAGFLPTAGIGAAMAMESAWVLASDLRHA
                     TVDTLGRVLDDYEAAQRPRVEAAQQTSRDLARWMFSTSGRLARARDLVMRMVSVKVAL
                     RPIQQLLDDRPTPGR"
     gene            4914..5519
                     /locus_tag="VK103_00865"
     CDS             4914..5519
                     /locus_tag="VK103_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLQ82058.1"
                     /translation="MTHQRQERMRPARRWRLVLAAATEFAEHGFEQASVNRILAACGM
                     SKSSLYHVVDSKADLLDLVVRDLAASIAGHWSPPAPDEFADDFWGTAGQVVAGLGALA
                     ATDTDFGLLGRVFHLSADSPATGEVFAAVGSWLDDVLAVGRRTGAVDDGLPADLQAQV
                     VLAVLRAFDEWTLRHVTEMDGPFARDLVDHQLAALRRLLTR"
     gene            complement(5631..>6374)
                     /locus_tag="VK103_00870"
     CDS             complement(5631..>6374)
                     /locus_tag="VK103_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HLQ82059.1"
                     /translation="QLTAARFSVTVGDPEYSETVPSGRVIRTEPRAGTELPPGSEVSL
                     VLSKGPERYEVPTLVGEKLTDVKAALTAAHLELGKVTEEWDEKVAKGVVLEASAEEGA
                     RVRPGTTVDLTVSKGKEPITVPDWTGKSAEDAKKALEKLGFTVKVSEKHDDSVDKGAV
                     ISQDPAKGTLHRGDTVSLVESLGPVMVTVPNVRYMKIDEAEATLKKAGFAVERKALIS
                     ATPLGLVSYTDPGGGKQARKGSTITIFYV"
BASE COUNT          887 a         2304 c         2222 g          961 t
ORIGIN      
        1 gttggtgcag gtcatcaggc cgtcgtacat gtagccgatc accttgctga cacggctctg
       61 cgcgaccgac aactgcttcg cgacccgctc gtacggggcc gcgatgctgc gcccgtcgtc
      121 acaggtctcc tggtcctcac cgacgccgcg ctgcatcagc tcgacgttga tccacagctc
      181 ggcgtccggg ttgtcgaggc gccgctggcc ctccagcatc gaggcgaagt agtcaccgga
      241 gttgccctgg taggcctcgg agtaggtcac cttcccgacg gtgtcccaca acggctcctc
      301 gacgatctgg tcgacctcgt cggggaagta cacccccatc ttgttggtgc cgcgggagtc
      361 ctgcggcgag atcacgatcc gagtgccgtc cggtgcggtc ccggcgagcc ggatgaaggc
      421 gtcgtcggcc atctcgatcg ggacgtccat gtcccggcgg gcgtcctggt acgggctgat
      481 catgatcgcc ctgcccggca tcaggtccgc ggccaggtgg tgctgcaggg tgtagagctc
      541 gtagccggca tcccaggccg cgttgtcctt catcgggatc tcggtgctct ggtacacccc
      601 gacccacgag tcgtagtggg cgtagcgctg ctgccagtcg gtgaacagcc ggctgctgaa
      661 cgcctcggtg tagtcgaacg cggcacggtc cggcagccac ggcgactccg gatccttgtc
      721 cggcaccggc atcccgatcc agaagtcgat gccgtacgcg tcggccgcgg tcagcatcga
      781 gtcgatcatg ttcgggctgt cggcggcacc gccggacacc agcaccaggt cgtactggtc
      841 gtgcgcggcg tcgcagccgc ggtcctcgcc gacgggcagc aggatccgct ggtacatccg
      901 accgttgtgc accacggtcg cgtcccgacc gttgtcggtg cagcgcacca ccgcgtcgcc
      961 gaggtgctcg gtgttggagt agtacaggtg gtgccggatg gtgctgtcgg gtacggcgtc
     1021 ggcggccgcc tgcacgcagg tccgaccgtc ctcgacgcaa tccacccact tcgggtcgac
     1081 cgtgccgtcg gcgagcagca cccggccctg ctcgtccacg ccgggcagct tgttgatccg
     1141 ggagccgaac gtgatggcgg tgtcggcgtt gatcgagtgc agggccgcca tcttcgccat
     1201 cgcatcgcac ggttcggtgt cctggaagat gaagtagccg gtgacggggg aggcgacctg
     1261 cggcgggatg cgccgcggcg gggtgccaca gcgtgctgcc agcgccgcct gctccggcgt
     1321 catcgccgtc gacgccgagg ggcccgaggg atcggcggag gcggacggca gggtcgcggt
     1381 cccggtggtc agcgcgagca gcagggcagc ggtggccgcc acccagtggg tcagccggcg
     1441 gggtgggcgg tgtcggacgg cgggtgcgtc ggtggcaggc atgggggcgg ccctcctcgg
     1501 tcgcgcgacg gcgctcgacc cgcgccgatc gtcgcactgg accctagggc tcgccgaacc
     1561 gtccgtgcaa ccccttgcac gcgttggtgg gcacggatcc gggggagcgg gacgtcccct
     1621 tgacacccca cccggacctg ccacaggctc gtcacgtccc agtgcagtgc ccccgatccc
     1681 ccacggtgag gaagcccccc atgcaccacc cctcgacgtc gaacccggcc gcggcgctca
     1741 cccggcgccg tctgttgggc gcagccggag ccaccaccgg cgccgccgtg acgtgggcgt
     1801 cggcccgtcc ggcgacggcc ggtccgcgcc cggtcgacac ccgcccgcgt ggtggtgccg
     1861 acagcccgca gtcctccgac gactcgctga cctggtcctc ccccgcggag gcaccgttcc
     1921 ggctggccgg cttctgctgg ttcgaccagg accgggtcca ccgtcgcctc ccggtggagc
     1981 cggactggcc gctccccgag gcggtcgacc ggctggccga caacaccgcc ggtggccaga
     2041 tccggttccg taccgactcc acccgggtcg cggtccgggt cagcttgtcc ggaccgcccg
     2101 gcatgaacca catgcccgcc accggccagt gcggtttcga tgcctacgtc ggcgacttcg
     2161 gtgaccagca gtactggggg accgcccggc cgacgttgga cgagaccgcg tacgaggcgg
     2221 tgttcttcac caccccggag ccgcagaccc gaacggtgac gctcaacttc ccgctctacc
     2281 aaggagtgga ggaggtgctc gtcgggctgg acgccggagc ctcggtcgag gcggcacccg
     2341 acttcgagcg gcccggacgg ctcgtggtgt acggcacgtc gatcacccag ggcgggtgtg
     2401 ccagtcgccc cggtgcggcc tacccggccg agctgtcgcg ccgactggcg atcgagacgg
     2461 tcaacctcgg cttctccggc agcggcaagg gtgagccgga ggtggcgcgg acgatcgccc
     2521 agatcgacga cgtcgcatgc ttcgtgctgg actacgagtc gaacgtcgcg accgccggcg
     2581 acatgcggcg cacgctgcgg cggttcatcc cgatcctgcg ggaacgccat cccgcggtgc
     2641 cgatcgtggt ggcctccaag accgcccgga tcccggaacg ctacgacccc gatgccctcg
     2701 ccgaccggct ggccaagcgc aaggcacagg cggatgtggt ggcccggctg cagcgggccg
     2761 gggacgaccg gctgcagttc ctcgacctgg gcgaccatga cctgagcgca tccagcgtcg
     2821 acggcaccca tctcaccgac ggtggcttcg tccagctggc cgacctgatc gagccctcgg
     2881 tacggcgatg gctgtcggcc tgaccgtccg tgacgcgcca ccgggatgac ccacgactag
     2941 ggtggggaca tgaagctcac ccacctcgga cactcggccg tcctcgtgga agtggcggac
     3001 cgccgactgc tgatcgaccc cggcaacttc tccgacgcgt ggcacggcgt caccgacctc
     3061 gatgccgtgt tggtcaccca cctgcacccg gaccacatcg acccggagca cgtccccgcg
     3121 ctgctgcagg ccaatccgga ggcccaggtg cgcgtcgagc cgggggtgat cagtacccac
     3181 cacccggccg gggccgagcc attgcgggcc gaggaggagt tgcgactcgg cgacgtcacg
     3241 gtccgtgcga tcggcggtga gcatgccgtc atccaccgcg acatccccat gatcggcaat
     3301 atcggcctgg tgatcagcgc accgggtgag ccgaccttct tccatcccgg tgacagcctg
     3361 gccgccgtcc cgaccggagt ggacgtcgtc ggtgtgcccg cctacggccc gtgggcagcg
     3421 atgaaggaga ccatcgactt cgcccgtgcg gtcggggcgc cgaaggggtt cgcgatccac
     3481 gaccggctgc tcaacgaccg gggcttcacg atgctgcacg accggatcgc ggagatgagc
     3541 ccgaccgcga tggtcgacct gcgggacgga tccccgtacg acccgggcga ccccgcctga
     3601 tcaacgtccc ggggtgggtc ggtcgtcgag caactgctgg atgggccgga gcgcgacctt
     3661 gacgctcacc atccgcatca ccaggtcccg tgctcgcgcc agccggccgc tggtgctgaa
     3721 catccaccgt gccaggtcgc gggaggtctg ctgcgcggcc tccacccggg gtcgctgggc
     3781 ggcctcgtag tcgtcgagca cccggccgag ggtgtcgacc gtcgcgtggc gcaggtcgct
     3841 ggccagcacc caggctgact ccatcgccat cgcggccccg atccctgcgg tggggaggaa
     3901 cccggccgcc gcgtcgccga gcagcaccgt acggcgatcc gcccagtggg gcgcacggca
     3961 gtcggccagc ggccagtagt acgggtcctc cgcggacgcc acggcggtca ggcagctgtc
     4021 gatccggggt gggacggtgc gcagtgcccg tcgggcccgg gtggcgaact ccgccggccc
     4081 gatcgcggtg cggccgtgcg ggccgcccag gaacaccccg agacgcccct gcactgggta
     4141 ggcgccgacg aagaacccct gcccccacag ctcactgccc agatcggggt cggcgtcctc
     4201 gggcgcccac accacccagc cgccccagcc ggtgtcgagc acgtcgacct gccgccccgc
     4261 ccggacgagc cgacgggtgg tggagtggat cccgtcggcg atgaccacca ggtcgaaggc
     4321 gaggtccacc cgccggtcat cgacgagcag ggtggcgacg gtgtggtcgt cggtctcggt
     4381 cagggccccg acggtggtgc cgaacgtcgc ggggcacccc gggctggcga ggctctcgat
     4441 caactcaccc cgggcgatgc cctggtagct gccgtacgcc gacaacagct cggacatcga
     4501 gtcgtcgcgg acctgccggc cacggctgtc gtggagtccg taccgggcga gcgggactcc
     4561 ccggggcagg tagtcgtgcc acaggcccag ggtgtcgaac accgggtcga ccatcggcat
     4621 catcgccaac atgtagccgg ccttgttctc gtcgagcagt gcgtcgacgt cgcgggcacg
     4681 gtccaccagc accgggtgcc gcccgtggtg gcgcaacagc tgcgccgcgg tgagcccagc
     4741 catgccggca ccgacgacga ggacccgcag gtcgtcggtc tgggccgcgg cgtactgctc
     4801 gggcagggtg gcgggctgga acatggcact ccctcgtcgg atgtggacca gctggtccac
     4861 atccgagcgt agcagtcatg gaccagctgg tccacacgtg ttagcgtccc gggatgaccc
     4921 accagcgaca ggaacggatg cggccggccc ggcgctggcg cctggtgctc gcggcggcca
     4981 ccgagttcgc cgagcacggc ttcgagcagg cctcggtgaa ccggatcctg gccgcctgcg
     5041 ggatgagcaa gagctcgctg taccacgtgg tcgactccaa ggccgacctg ctggacctgg
     5101 tggtacggga cctggccgcc tcgatcgccg ggcactggtc accaccggcc cccgacgagt
     5161 tcgccgacga cttctggggc accgccggac aagtggtcgc cgggctcggc gcgctcgcgg
     5221 ccaccgacac cgacttcggt ctgctgggcc gggtgttcca cctcagcgcc gacagcccag
     5281 ccaccgggga ggtgttcgcc gcggtggggt cctggctcga cgacgtgctg gccgtcgggc
     5341 gtcgcaccgg ggccgtcgac gacgggctgc cggccgacct gcaggcgcag gtggtgctgg
     5401 ccgtgctgcg cgccttcgac gagtggacgt tgcgccatgt caccgagatg gacgggccgt
     5461 tcgcccgcga ccttgtcgac caccagctcg cggcgctgcg ccggctgctc acccgctgat
     5521 caccccgctg atcaccccgc tgatcacccc gcccacgcat gggttgtgcg gccacgcagc
     5581 agctgcagct gctgcgtggc cgcacaaccc atgcgtgagc ggggccgggg tcacacgtag
     5641 aagatggtga tcgtcgaccc cttgcgggcc tgcttgccgc cgcccgggtc ggtgtagctc
     5701 accaggccga gcggggtggc cgagatcagc gccttgcgct ccacggcgaa cccggccttc
     5761 ttcaaggtgg cctcggcctc gtcgatcttc atgtagcgca cgttcggcac cgtgaccatc
     5821 accgggccga gcgactccac cagcgacacc gtgtcgccac ggtgcagggt gcccttcgcc
     5881 gggtcctgag agatcaccgc gcccttgtcg accgagtcgt catgcttctc gctgaccttg
     5941 acggtgaagc cgagcttctc caacgccttc ttcgcgtcct cggccgactt gccggtccag
     6001 tccgggacgg tgatcggctc cttacccttc gacacggtga ggtcgaccgt cgtgccgggg
     6061 cgcacccgtg ccccctcctc ggccgacgcc tccagcacca cgcccttggc gaccttctcg
     6121 tcccactcct cggtgacctt gccgagctcc aggtgggctg cggtgagtgc cgccttcacg
     6181 tcggtgagct tctccccgac gagcgtgggc acctcgtacc gttccggacc cttcgacaac
     6241 accagggaca cctcactgcc cgggggcaac tcggtgcccg cccgcggctc ggtgcggatc
     6301 acccggcccg acggcacggt ctcggagtac tccgggtcgc cgacggtgac ggagaaccgc
     6361 gcggcggtga gctg
//
LOCUS       DATGUK010000056         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_297373,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000056 DATGUK010000000
VERSION     DATGUK010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297373"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VK103_00875"
     CDS             complement(<1..1138)
                     /locus_tag="VK103_00875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLQ82060.1"
                     /translation="MVLLALNLRAAVGSLGVLLGPVSDALGMSATVGGVLTTLPVLCF
                     AGFGVFTNPIVRALGLHRTAGFAIGLITVGLAARALSDSTWVFVATSALALAGGAVGN
                     VILPPLVKLHFPNRVGTVSALYTASIMAGGSLSAMITVPVANAAGGWRAGLMIWAVTA
                     AATMLPWLGLLRHDVRAATRVRSGIGFSTVIRSPLAWVMAVFFAMQSAQAYAQFGWLP
                     RMLVDAGLASDTAGLVQGILTGVGIPMALSLPYLMRRLGDRPYLPWLFGVLTIVGWFG
                     VLYAPTAAPWLWAVLLGIAGSAFPWVMTMLGYRTRSHDGTAAMSSFVQGIGYLFAAVG
                     PFGTGLLHDVTGSWTIPVYALAALGVGIIVLGTVICRPTMFEDQI"
     gene            complement(1844..>2357)
                     /locus_tag="VK103_00880"
     CDS             complement(1844..>2357)
                     /locus_tag="VK103_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82061.1"
                     /translation="RALLARRCALATPVTSGVVVTEGLARGVVLVRVGLARGGSAAPA
                     ASATPAAAAGLTRVLVRLGGAVCMVWLVAGATAPVAILGRGLATASVLTATIAVIAAA
                     DRAVLLRRRLDRLRDLEQRSRKRRSRLRRQSVDGGGAHRRAPVRREGRIRRSRRSRRR
                     LYRFRGMAAR"
BASE COUNT          421 a          802 c          835 g          299 t
ORIGIN      
        1 caatttggtc ctcgaacatg gtcggccggc agatcaccgt cccgagaact atgatcccca
       61 cgccgagtgc ggcgagcgcg tacaccggga tcgtccagga gccagtgaca tcgtgcagca
      121 gaccggtgcc gaacgggccg acggcggcga acaggtatcc gatgccctgt acgaagctgg
      181 acatcgccgc ggtgccgtcg tgggagcggg tccggtatcc cagcatcgtc atcacccatg
      241 ggaacgccga gccggcgata ccgagcagca ccgcccacaa ccacggtgcg gccgtcgggg
      301 cgtacagcac accgaaccag ccgacgatgg tgaggacgcc gaacagccac ggcaggtacg
      361 ggcggtcgcc cagccgacgc atcagatacg gcaacgagag cgccatcgga atgccgacgc
      421 ccgtcaggat gccctgcacc agccccgcgg tgtcgctggc cagcccggcg tcgacgagca
      481 tccgggggag ccagccgaac tgcgcgtacg cctgcgccga ctgcatcgcg aagaacaccg
      541 ccatcaccca ggcgagcggt gagcggatca cggtgctgaa cccgatgccg gaccgtaccc
      601 gggtcgcggc ccgcacatcg tgccggagca gcccgagcca cggcagcatt gtcgctgccg
      661 cagtcaccgc ccagatcatc agtcccgcgc gccagccgcc ggccgcgttc gcgaccggaa
      721 cggtgatcat tgccgacaac gagccgccag ccatgatcga tgcggtgtag agcgcgctga
      781 ccgtaccaac cctgttcggg aagtgcagct tgaccagcgg cggcaggatg acgttcccga
      841 ccgcaccgcc tgcgagggcg agggccgagg tcgcgacgaa gacccatgtc gagtcggaga
      901 gtgcccgggc ggccaagcct acggtgatga gtccgatggc gaacccggcc gtacggtgca
      961 gcccgagcgc gcggacgatc gggttcgtga atacgccgaa gccggcgaag cacagcaccg
     1021 ggagtgtggt caggacgccg ccgacggtgg cggacatccc cagggcgtcg ctgaccggac
     1081 cgagcagtac gccgagactg ccgacggcag cgcgcaggtt cagcgcaagc agcaccatgg
     1141 cggcaagtac gaagatgcga gcgttccggg actcgaggcg gacgctcggg aggctcaccg
     1201 gacacattct tcccccgcga cttggttggc ccctgacccg ggtgctgcca cctgtggtcc
     1261 acctgtggtc cacctgtggt cctccgccag agtccggcgc ggtgtggggt ccggacgagt
     1321 ttcacgtcgt gtgcgatact ggaggtggtt gccgggcgga aagaccgccc gagtgatccg
     1381 acgatgttct cgtcggactg gcggctgaga ggtcacagtt cgacacggtc ggtgaccgac
     1441 gctcccgacc gcgacgcgtc gccggaccgc gccctcgcca gggtgcgggt gcacggtgac
     1501 agcgaccgcg gcggtgtgtg gtcgccgcgt gacaggccga cctggtggga gggcatcgcc
     1561 gaagcccgag aaacttcggg ccgaccgcag gcttgatgcc cgccggaggt gcattccacc
     1621 tctggatgcg ggcagttgac ggccccgtgg gtctcgttcg gcgaggctcg ggggcgaagg
     1681 agagcagatg ctcgacgaac cgaaggagaa cggcaccgga agcacaggcg acgatgacgt
     1741 ggccgatccc ggtgtgcgcc agaacccaac aacggcgacc cgcgaacatc gtgcggtgtc
     1801 tcggcccgcc ggcccccctg ccgggtcccc cggtgggcac gcttcatcgg gcggccatgc
     1861 cgcggaaccg gtagagacgc cgtctggatc gccgactgcg gcgaatccgg ccttctcggc
     1921 ggaccggagc gcgccggtga gcaccgccgc cgtcgacgct ttgccgtcgg agacgagaac
     1981 gccgtttgcg gctccgctgt tcaaggtccc ggagcctgtc aagtctccgc cgaagaagga
     2041 ccgcccggtc ggcggcagcg atgacagcga tggtcgcggt gagcacagaa gcagtggcga
     2101 gtccgcgccc gagaatggcg acgggagcgg tggcccccgc gacgagccag accatgcaca
     2161 cagctccacc gagtcggacg aggactctgg tgagtcccgc cgccgccgcc ggcgtcgcgg
     2221 aggccgccgg cgccgcagat cctcctcggg cgagtccgac tcgcacgagg acgactcctc
     2281 gggcgagtcc ttcggtgacg accacaccgg aggtaaccgg agtcgccaga gcacaccggc
     2341 gagcaagcaa ggctcgg
//
LOCUS       DATGUK010000057         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_994390,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000057 DATGUK010000000
VERSION     DATGUK010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994390"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VK103_00885"
     CDS             complement(<1..776)
                     /locus_tag="VK103_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HLQ82062.1"
                     /translation="MVELREDDLFTLAGSLSELLGAPVTIEDPDTTVLAYSGGAQAVD
                     DARIGTILGRQVPSEYRRMLADAGVFERLRTDDDVVYVDLGKEQMTPRAVIAVRDHDD
                     LLGSIWAAVHAPPTAHQESVLRSAAPVVAEAMLRERDRSGSANRRSAHHMEQLLTGGD
                     RAVRTADELGMRGRQTVAAVARRDPGEPVSSRMLGSVRLHLSAVCPGSLVAELDDRVY
                     VAAPVEESGMRRILADYLTRARNLDDLLACVGRAVASVAEA"
     gene            complement(872..1195)
                     /locus_tag="VK103_00890"
     CDS             complement(872..1195)
                     /locus_tag="VK103_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HLQ82063.1"
                     /translation="MAWFLLSAAIVAEVAGTLSLRASDGFTRGWFTLASLAGYAVALV
                     LLGFVLKHGMPVGVAYGIWAATGVALVAVLGRVLFGDALTVPMAFGLALIMAGVLLVE
                     TGATR"
     gene            complement(1230..1736)
                     /locus_tag="VK103_00895"
     CDS             complement(1230..1736)
                     /locus_tag="VK103_00895"
                     /inference="COORDINATES: protein motif:HMM:NF037679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLQ82064.1"
                     /translation="MLDAAARVARRDGAGAVTVSAVVAEAGVSRGGLLYHFPGKDDLL
                     RGLVERELAAFRSVLEAGDDTAARLRAYRDACMIGPNAAEDRVAVLVALSEAPDLLAS
                     WISYVRELDQLDLAPAGGQRRTGEGMPAVVARLALDGLWLSDLVDPDRFTTTQRAEVL
                     RTLGVPQD"
     gene            complement(1991..>2339)
                     /locus_tag="VK103_00900"
     CDS             complement(1991..>2339)
                     /locus_tag="VK103_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82065.1"
                     /translation="LYEYHSAINTADYDGAMGLLTPDFAAEIFPDERQWHGAYETTYD
                     DQLEVVEATRDGDQARVRLRFRSQQESGYGPKGARRATCLWWDIDYRVELHDGEWLID
                     GASGHRDPAWTRC"
BASE COUNT          374 a          851 c          776 g          338 t
ORIGIN      
        1 gcctcggcga ccgacgccac cgcccggccg acgcaggcca gcaggtcatc caggttgcgg
       61 gcacgggtca ggtagtccgc gaggatccgt ctcatccccg actcctcgac gggtgccgcg
      121 acgtagaccc ggtcgtcgag ctcggcgacc agcgagccgg ggcagaccgc gctcaggtgc
      181 agccggaccg aaccgagcat ccgggaggac accggctcgc cggggtcccg ccgggccacg
      241 gccgcgacgg tctgccgccc gcgcatcccc agctcgtcgg cggtgcggac ggctcgatcc
      301 ccgccggtga gcagctgctc catgtggtgg gcactgcgcc ggttcgccga tcccgaacga
      361 tcgcgctcgc gcagcatcgc ctccgccacg accggcgccg cgctacgcag caccgactcc
      421 tgatgcgccg tcggcggggc gtgcacggcg gcccagatgg acccgagcag gtcgtcgtgg
      481 tcgcggaccg cgatcaccgc tcgtggtgtc atctgctcct tcccgaggtc gacgtagacc
      541 acgtcgtcgt cggtgcgcaa tcgctcgaag accccggcgt cggccagcat ccggcgatac
      601 tcactcggca cctgccggcc gaggatcgta ccgatccggg catcgtcgac cgcctgggcc
      661 ccaccggagt acgcaagcac cgtggtgtcg gggtcttcga tcgtcaccgg cgcgcccagt
      721 agttccgaca gcgagcccgc cagggtgaac aggtcgtcct cgcgcaactc caccatgacc
      781 ggcagggtac gggagccgcg gcggcttcgg ggagtaccga tcgggagtat cgggagtacc
      841 gaggcgtccg ttcggccggg cccggtccgg ctcagcgggt cgccccggtc tccacgagca
      901 gcacgccggc catgatcagc gccagcccga acgccatcgg caccgtcaac gcatcaccga
      961 acagcacccg acccagcacc gcgaccagcg cgactcccgt ggcggcccag atcccgtacg
     1021 ccaccccgac cggcatgccg tgcttgagca cgaagcccaa caggaccagc gccaccgcgt
     1081 agccggccag gctcgcgagg gtgaaccacc cgcgggtgaa cccgtcggag gcccgcagcg
     1141 agagcgtgcc ggcgacttcg gcgacgatcg cagcactcag caggaaccat gccatcggca
     1201 ctccttcagt cctgttcagt ccagtaccgt cagtcctggg gcactcccag cgtgcgaaga
     1261 acctccgcac gctgggtggt ggtgaagcgg tcggggtcga cgaggtcgct gagccacagg
     1321 ccgtccagcg caagtcgggc gaccaccgcg ggcattccct caccggtgcg ccgttgtccg
     1381 ccggcagggg ccaggtcgag ctgatccagc tcgcggacgt aggagatcca ggacgccagc
     1441 agatcgggcg cctccgagag cgccaccagg acagctactc tgtcttcggc cgcgttgggg
     1501 ccgatcatgc acgcgtcacg gtaggcacgc agccgcgcag ccgtgtcgtc ccctgcctcg
     1561 agcacggagc ggaaggcggc gagctcgcgc tcgacgaggc cacggagcaa gtcgtccttt
     1621 cccggaaagt gatagagcag accaccgcga ctcaccccgg cctcggcgac cacggcggag
     1681 acggtgaccg ctccggcacc gtcgcgccgg gcaacccggg cggcggcgtc cagcatccgc
     1741 gctcgcgtgg acgagggttt ggtgttagcc atgaaggtta ctgtaccgta cgtacggtac
     1801 agtaactagt cgcctggaag gttcgcctcc gggtctgctg ggcctcagtc atgtcacgct
     1861 accggtcgta ccggttccac ccacatcaca tcgcggccgc gaatcttgcg ggctgaaagg
     1921 tgattccgtc cggcccgaac gtgagaatgc tgcggctgcc atcacgccac cggcgaggta
     1981 ggccgcagca ctaacaccgt gtccacgccg ggtcacgatg cccggaggcg ccgtcgatca
     2041 gccattcgcc gtcgtgcagc tcgacccggt agtcgatgtc ccaccacaga caggtcgccc
     2101 ttctcgcccc cttgggtccg taaccagatt cctgctgact gcggaaccgg agccggactc
     2161 gcgcctggtc gccgtcccgg gtggcctcta cgacctcgag ctggtcgtcg taggtcgtct
     2221 cgtacgcgcc gtgccactgc ctctcatcgg ggaagatctc tgctgcgaag tcgggggtca
     2281 gcaggcccat cgccccgtcg tagtcggcgg tgttgatcgc cgagtggtac tcgtacaac
//
LOCUS       DATGUK010000058         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_531960,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000058 DATGUK010000000
VERSION     DATGUK010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531960"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VK103_00905"
     CDS             complement(<1..525)
                     /locus_tag="VK103_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLQ82066.1"
                     /translation="MHTPSTVTRRTIVLGLGAAAALPLASCVGEGGAPTTAPKGAATG
                     PVEGRVEFWTINLRKNFQMYFEDLISTFESENDGVTVEWVDVPGKDMTAKYLSAIASG
                     KVPDVINVDSKNLGQLNATLAGLDDVYSDDDLSIFQPGLVDGLRVDNTLKGIPWYNSG
                     TRICMYRKSTMDKIG"
     gene            complement(575..>2453)
                     /locus_tag="VK103_00910"
     CDS             complement(575..>2453)
                     /locus_tag="VK103_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82067.1"
                     /translation="DHPNIGGLIIDDFDTNTPQRHSPWSFVFTPDYVRQMSAAAKALS
                     PDFTIHGVVYQPAMDVGSAFRGALDGVIFPYRGETSTPGTWDASSARAEGEVYGDLAH
                     CASDRCLQFHGDGSSTDGTAAGATRQISITGGGEHRLTMKVNNDNYRNPCEDGRCFEF
                     SMPGHHPTAAGDHVAISQTVEVSGDGPAELSFFLGDSNRRMVTGYHFVEALIDGEVVA
                     RRDLAGAEEAGRISVDVTDQLSGKSEVELTLRLFDEQGVTNFGITAWVDDVALTGATV
                     ADASFDDRNSGAWQESQVGEQMSALYTAGAYVLETIINGEVVGTHAIEGYQGWSEVTQ
                     DLTTPLNGLSEAALEVRLRAEDSERTARTVWVDDLAIDGTTATAENFDEADWDFHQRG
                     DLQSLQVASFDTLWMVYASRLASDPPGHQPSADYIAEVQEIGLDLMQEGLFDGSLIYV
                     LNLSDPVTSPAGEERARIGELYGDYAATDLASCDTVLHGRQHDLTVADGRTCLVGGTA
                     LGPTTVADGAELVAMDATFRGSVTVDGGAVALCGSRVAGSMTVRDAREVRLGATTSLC
                     AGNTLAGSAAVDGTSSTLVVAALTLRGALSCSDNVALDQRGTTSTIDGSASGDCAVLA
                     G"
BASE COUNT          383 a          807 c          834 g          429 t
ORIGIN      
        1 accgatcttg tccatggtgg acttgcggta catgcagatc cgagtgcccg agttgtacca
       61 cgggatgccc ttgagcgtgt tgtccacgcg caatccgtcc accaggcccg gctggaagat
      121 ggacagatcg tcgtcggagt acacatcgtc gagcccggcg agcgtggcgt tgagctgccc
      181 cagattcttg gagtcgacgt tgatcacgtc ggggaccttg ccggaggcga tcgcgctgag
      241 gtacttggcg gtcatgtcct tgccggggac gtcgacccac tcgacggtga ccccgtcgtt
      301 ctcggactcg aaggttgaga tcaggtcctc gaagtacatc tggaagttct tgcgcagatt
      361 gatcgtccag aactcgaccc tgccctcgac cggccccgtc gcggcgccct tcggcgcggt
      421 ggtgggcgcg ccgccctcgc cgacgcagct ggccagcggc agcgccgcag ccgctccgag
      481 accgagcacg atcgtgcgtc gagtgaccgt ggatggtgtg tgcatggttc tccttcgtga
      541 gctggatggt ggggtcggcg gtcccgctgt gcggtcagcc ggcgaggacc gcacagtctc
      601 cgctggccga cccgtcgatg gtgctggtgg tgccgcgctg gtcgagcgca acgttgtcgg
      661 agcaactgag cgcgccgcgc agggtcagtg cggcgaccac cagcgtggag ctggtcccgt
      721 cgacggcggc cgagccggcc aaggtgttcc cggcgcacag tgaagtcgtg gcaccgagcc
      781 ggacctcacg ggcgtcgcgc acggtcatcg acccggcgac tcgcgacccg cacagcgcca
      841 ctgctccacc atcgacggtc actgagccgc ggaaggtggc gtccatcgcc accagctccg
      901 caccgtcggc aacggtcgtc ggaccgagcg cggtgccacc gaccagacag gtccggccgt
      961 cggcgacggt gaggtcgtgc tggcggccgt ggagcacggt gtcgcagctc gccaggtctg
     1021 tcgcggcgta gtcgccgtac agctcgccga tgcgggcgcg ctcctcaccg gccggggagg
     1081 tcaccgggtc ggacaggttg agcacataga tcagggatcc gtcgaagagc ccctcctgca
     1141 tcaggtccag cccgatctcc tgcacttcgg cgatgtagtc ggccgacggc tggtgtcccg
     1201 gtgggtcgct ggcgagtcgg gaggcgtaga ccatccacag cgtgtcgaag ctggcgacct
     1261 gcagtgactg cagatcaccg cgctgatgga agtcccagtc tgcttcgtcg aagttctcgg
     1321 cagtcgcggt cgtcccgtcg atcgccaggt cgtcgaccca caccgtacgg gcggtccgct
     1381 ccgagtcctc ggcccgcagc cgaacctcca acgctgcctc cgaaagcccg ttcaggggcg
     1441 tggtcaggtc ctgggtcacc tcggaccagc cctggtaccc ctcgatcgcg tgggtgccga
     1501 cgacctcacc gttgatgatc gtctccaaca catatgcccc ggctgtgtac agggcactca
     1561 tctgctcgcc gacctggctc tcctgccaag cgccggagtt gcgatcgtcg aacgaagcgt
     1621 cagcgacagt cgcgccggtg agggcaacgt cgtcgaccca ggcggtgatg ccgaagttcg
     1681 tcacaccctg ctcgtcgaac agtcgcagcg tcaactcaac ctcggatttg cccgacaact
     1741 gatcggtgac gtcaacggag atccgtccag cttcctcggc cccggccagg tcgcgccggg
     1801 ccacgacctc tccatcgatg agtgcttcga cgaagtggta gccggtgacc atgcggcggt
     1861 tgctgtcccc caggaagaac gacagctccg ccgggccatc gcccgacacc tcgacggtct
     1921 ggctgatcgc gacgtggtcg ccggccgcgg tggggtgatg gcccggcatc gagaattcga
     1981 aacagcgtcc gtcctcgcag ggattgcggt agttgtcatt gttgaccttc atcgtcaacc
     2041 ggtgctcgcc cccaccggtg atggagatct gtctggtggc gccagccgcc gtaccgtccg
     2101 tcgagctgcc gtcgccgtgg aactgcaggc aacgatcgga ggcgcagtgc gccagatcgc
     2161 cgtacacctc gccctcggcc cgcgcggatg aggcgtccca ggtgccggga gtgctggttt
     2221 cgccgcggta ggggaagatc acgccgtcga gtgcacctcg gaaggcggag ccgacatcca
     2281 tcgcaggttg gtagaccacg ccgtggatcg tgaagtccgg gctcagcgcc ttggccgcgg
     2341 ccgacatctg acgcacgtag tccggggtga acacgaagga ccacgggctg tggcgttgtg
     2401 gcgtgttggt gtcgaagtcg tcgatgatca ggccaccgat gttgggatgg tcg
//
LOCUS       DATGUK010000059         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_774529,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000059 DATGUK010000000
VERSION     DATGUK010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774529"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VK103_00915"
     CDS             <1..615
                     /locus_tag="VK103_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82068.1"
                     /translation="VLVLLTLPLTPRTAAQLALRDLRDVLARRLDVVADKLGGDQLPT
                     TPDWREALPDVADPMWHARTEVRAMALADRYNWRTRLFHTGALRYDVAATRRQLEALE
                     RIAVLVDDLSLTVADRERRDADPEVWPGLGGPARDATIRAVRELARVLRSDGAGEVDP
                     EALQAADDAAESLQHSVQSGEGASLSSAGMTVAHSIRRALWTIR"
     gene            726..1985
                     /locus_tag="VK103_00920"
     CDS             726..1985
                     /locus_tag="VK103_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLQ82069.1"
                     /translation="MTLDPAVPRLPGPDHTIPLLREGYQFVGRRCDARGVDGFVARLR
                     GRDTLCVRGTDGAELAYGEGRVTRAGAIPAAVRHLLQDDDSVQGLDGAAHQRRRDLFL
                     EVLGPTDTARVVDLVRDRWACAVRDWPIGVTKSLGEVMDRVLAGAALEWTGLPHDRGT
                     VTRLTGELASMVDHAGLLDGPSVGSLAGNWRARARRRGTERWVEEQVRALRDRPPAEM
                     TGPAVRVALHREGGAALDTATAAVELINLLRPVVAICRFVVFTAMALARHPQWQQPLA
                     DGDHAAAVTVAHEVRRTHPFFPAILTIADEGAHWGAIPVPAGTLVLFDLYGTNHHPEL
                     WPYPWAFRPERHDDPAAVARLVPQGWGDPVSSHRCPGEPMTVQVVAAMACELARFPHR
                     LPMQDLTLPLNHLPATPAGGLRVTRTG"
     gene            complement(1962..>2213)
                     /locus_tag="VK103_00925"
     CDS             complement(1962..>2213)
                     /locus_tag="VK103_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82070.1"
                     /translation="TFQLTTAAGEQHTFDTFECAISTVAPRCQHCDVQVIGHGVQHGT
                     DVYCCAHCAREAGVDSVSDNSAGSPTRRGACRSARTGDP"
BASE COUNT          280 a          774 c          852 g          307 t
ORIGIN      
        1 gtgctggtgc tgctgaccct gccgctgacg ccccggaccg cggcgcaact ggccctgcgc
       61 gatctgcggg acgtgctcgc ccggcggctg gacgtggtgg ccgacaagtt gggcggcgac
      121 cagctgccca ccaccccgga ctggcgggag gcccttccgg acgtggctga tccgatgtgg
      181 cacgcccgca cggaggtgcg cgcgatggcg ctggcggacc ggtacaactg gcgcacccgg
      241 ctgttccaca ccggggcgct gcggtacgac gtcgccgcca ctcgtcgcca gctggaggca
      301 ctcgagcgga tcgccgtgct ggtggacgac ctgagcctca ccgtcgccga tcgggagcgc
      361 cgggacgccg accccgaggt gtggccgggc ctcggcgggc cagcgaggga cgcgacgatc
      421 cgcgcggtac gggaattggc ccgggtgctg cgctcggacg gggcggggga ggtcgacccc
      481 gaggcgctgc aggccgccga tgacgccgcc gagtcgctgc aacacagcgt gcagtccggg
      541 gagggagcga gcctgtcgtc ggcgggcatg acggtggcgc actcgatccg acgtgcgctg
      601 tggaccatcc ggtgactccg tccccgatcc tggcccggag ccgatgacgg ctgagaccgg
      661 gcctctcgac gtctcgctcg agggtgggcg gttttcggcc gtccggtccg gggtaccgga
      721 cggccatgac actcgacccg gccgtcccgc gtctgccggg accagaccac acgatcccgc
      781 tgctgcggga ggggtaccag ttcgtgggcc gccgttgcga tgcccgcggg gtggacggtt
      841 tcgtcgcccg gctgcgaggg cgcgacaccc tgtgcgtccg cggcacggac ggtgccgaac
      901 tggcgtacgg ggaggggcgc gtcacgcggg cgggggccat cccggccgcg gtccggcacc
      961 tgctgcagga cgacgacagc gtccaagggc tcgacggtgc ggcccaccag cgacgccggg
     1021 acctgttcct ggaggtgctc ggtccgacgg acacggcccg cgtcgtggac ctggtccgtg
     1081 accgctgggc ctgcgcggtg cgggactggc cgatcggtgt caccaaatcg ctcggggagg
     1141 tgatggaccg ggtgctggcc ggtgccgcgc tggaatggac cggcctgccc cacgaccggg
     1201 ggacggtgac ccggctgacg ggggaactcg cgtcgatggt cgaccatgcc ggactgctcg
     1261 acggtccgtc ggtcggttcg ctcgccggca actggcgtgc ccgggcacga cgccggggca
     1321 ccgagcggtg ggtcgaggag caggtgcgcg ccctgcgcga ccggccaccg gccgaaatga
     1381 ccggcccggc ggtgcgggtg gcactgcacc gggagggcgg ggcagccctg gacaccgcca
     1441 cggccgcggt ggagttgatc aacctcctgc gtccggtggt ggcgatctgc cggttcgtgg
     1501 tgttcaccgc gatggcgttg gcccggcatc cgcagtggca gcagccgctc gccgacggtg
     1561 accacgccgc tgcggtcacc gtggcacacg aggtgcgccg cacccacccg ttcttcccgg
     1621 ccatcctcac catcgctgac gagggggccc actggggcgc gatcccggtg ccggccggga
     1681 cgttggtcct gttcgacctg tacgggacca accaccatcc ggagctgtgg ccgtacccgt
     1741 gggccttccg gccggagcgc cacgacgacc cggctgcggt cgcccggctg gtgccacagg
     1801 ggtggggcga cccggtcagc agccaccggt gcccggggga gccgatgacg gtgcaggtgg
     1861 tggccgcaat ggcgtgcgag ctcgcacgat tcccgcaccg gttgccgatg caggatctca
     1921 cgctgcccct caaccacctg ccggcaaccc cggccggcgg gctacgggtc acccgtacgg
     1981 gctgagcggc acgccccgcg ccgggtcggt gacccggcgc tgttgtcgct caccgagtcc
     2041 accccggcct cgcgggcgca gtgggcgcag cagtagacgt cggtgccgtg ctgcacgcca
     2101 tgtccgatca cctgcacatc gcagtgctgg cagcgcggtg ccaccgtgct gatcgcgcac
     2161 tcgaaggtgt cgaaggtgtg ctgctcaccg gccgcggtgg tcagctggaa ggt
//
LOCUS       DATGUK010000060         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_189359,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000060 DATGUK010000000
VERSION     DATGUK010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189359"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VK103_00930"
     CDS             complement(<1..1026)
                     /locus_tag="VK103_00930"
                     /inference="COORDINATES: protein motif:HMM:NF040070.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HLQ82071.1"
                     /translation="MAAWLGGGSGSATDAFRAGVIGWLAGQGSGVNVSQTSVTAVPLG
                     VLALVVMAVYTLARRLGAGSSITRLRHVLSFGATAGVCYAVLLALAASTVRVGGVTVS
                     TGRAAVSGLVLAGVFAGLGAAQSGGLLAEGAARLPVEVRRVTRGAAYGAGLLLFAGLV
                     VFATSLALDTKTLGRLWASLDPGLIGGVGLACLCLLLAPNLVLWAVAVLLGPGVAVGV
                     DTSVTLTEVSLGQLPAFPLLAALPEPGAQPGWALALAALPLLAGFAAGFVAADSAPGW
                     ASAIVQGAAAGVAGAFFVSALLALSSGSIGPGRMAEVGPDPLICLGMALLTLGAGGTA
                     GGALGHYR"
     gene            1442..>2139
                     /locus_tag="VK103_00935"
     CDS             1442..>2139
                     /locus_tag="VK103_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82072.1"
                     /translation="MGFLRTEAWRLSVFERGKHLWRRRHEEDSDLALITALQKLSPSS
                     RRLIVLQTIGEVDPETAAREVQMTVPAAAEAVSGAVETLQSTMATGLGDIEERLRDLA
                     NISDRISLPRPHGLRREARRRNRRRTLGAVVTAAVAVALAGIVATENPPMSQTDASPT
                     RQQFGDEQAPATPKVVEIDNEQMLDVKTVGALGKPKSAHPTEWEASEVASGDASSEPF
                     TICAPRRFSDRKAR"
BASE COUNT          388 a          789 c          733 g          229 t
ORIGIN      
        1 acgataatgg ccgagcgcgc caccggcggt gccacccgca cccaacgtga gcagcgccat
       61 acccagacag atcagcgggt cggggccgac ctcggccatc cggccgggac cgatcgaacc
      121 actcgagagc gccagcagtg ccgagacgaa gaacgcaccg gcgactccag cggcagcgcc
      181 ctggacgatc gccgacgccc aaccgggggc tgagtcggcc gcgacgaagc cggccgcgaa
      241 cccggcgagc agcggcaacg cggccagcgc gagcgcccac cctggctgag cgccgggttc
      301 gggcagcgcg gccagcaacg gaaacgccgg cagctggccg agcgacacct cggtcagcgt
      361 caccgaggtg tcgacgccga cagcgacacc gggcccgagc agcaccgcga ccgcccagag
      421 gaccaggttc ggcgcgagaa gcaggcacag acacgcgagt cccacgccgc cgatcagtcc
      481 ggggtcgagc gaggcccaca acctaccgag cgtcttcgtg tccagcgcga gggaggtggc
      541 gaataccacg agccccgcga acagcagcag tccggctccg tatgccgcgc cgcgggtcac
      601 cctgcgcacc tcgacgggca gccgggcggc gccctcggcc aacagtccgc ccgactgtgc
      661 ggcccccagg ccggcgaaca ccccggcgag caccagcccg gacacggccg cccgccccgt
      721 actcacggtc accccgccga cccggacggt cgacgccgcc agcgcgagca gaaccgcgta
      781 gcagaccccc gccgtcgcgc cgaagctcag cacgtgacgc agccgcgtga tcgaggagcc
      841 cgccccgagc cgccgagcca gggtgtacac ggccatcacg acgagggcga gcaccccgag
      901 cggaacggcc gtcaccgatg tctgggaaac attcacaccg ctgccttgcc cggcgagcca
      961 gccgatcacc cccgcccgga acgcgtccgt ggccgatccg ctgccgccac ccagccaggc
     1021 cgccatcgcg agcccatggc cacgatcagt cctccggccg cggtgaccac gctcaccacg
     1081 agcgccgaca gtgccagcgg acggggcgcg tgatcctgcg aggaccaccc ccaaccgcca
     1141 gcggctcgtt ggagcagatc ggtcatcgct actatcgtcg ctgtaacgtg gaccggtcgg
     1201 ccgaaggcac gccgcacggt gcggccagat tccacgcaac cggaaccagt tcatccaagc
     1261 cgccaacgga gggtagatgt cccgcgtcga agagttcgac accttctacg cctccacccg
     1321 gcggcacctg ctgcacgtga cgtacgcgtt gtgcggcgac cggaacgtcg ccgcggcagc
     1381 ggtcgcggac acgtaccgga atgcctggca gcggtggccg aagctgcgtg ccggtgaccc
     1441 ggtgggcttc ctgcgtacgg aggcctggcg gctgtcggtc ttcgagcgtg gcaagcactt
     1501 gtggcgccgc aggcacgagg aagacagcga cctcgcgctg atcacggccc tgcagaagct
     1561 gtcgccgtcg agtcgccgcc tgatcgtgct gcagacgatc ggggaggttg acccggagac
     1621 ggctgcccgc gaggtccaga tgacggtgcc tgcggccgcc gaggcagtct cgggggcggt
     1681 ggagacgctg cagtccacga tggcgaccgg gctgggcgac atcgaggagc ggctgcgcga
     1741 cctggcgaac atctccgacc ggatcagtct tccccggcca cacgggctgc gccgggaggc
     1801 gaggcggcgc aaccgtcgac gtacgctcgg cgcggtcgtc accgctgcgg tggcagtggc
     1861 actggcggga atcgtcgcca ccgagaaccc gccgatgagc cagaccgacg cgtctccgac
     1921 gaggcaacag ttcggcgacg agcaggcgcc agcgacgccg aaggtcgtcg agatcgacaa
     1981 cgagcagatg ctcgacgtca agacggtcgg ggcactcgga aagccgaagt ccgcacaccc
     2041 gactgagtgg gaagccagtg aggtagcgtc cggggacgcg tcctccgagc cgttcacgat
     2101 ctgcgccccg cgtcgattct ccgaccgcaa ggctcgaca
//
LOCUS       DATGUK010000061         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1354296,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000061 DATGUK010000000
VERSION     DATGUK010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354296"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1679)
                     /locus_tag="VK103_00940"
     CDS             complement(39..1679)
                     /locus_tag="VK103_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLQ82073.1"
                     /translation="MKPITRRTALGLGAAAVAAPLLASCGGAGSDSETQDKIDNPDAN
                     INKTGMPIVKESITLHMMTRHRPSTADDWNTVKSVKKLEELTNIKVDWGPVPWEGAAE
                     KRNLALASGDYPAILNRTAVSAVDLAKYGEQGTFIALNELIDEYMPNLKGVMDKYPDI
                     AKGMTMPDGNIYSMPTIYDPAFDSLMMQQKLWIRKDWLDDFGMSEPETLEEFEAFLTE
                     VKGKNPVKKGSPAAIPFTDGSAIGWLVQMFWGAFGIGTRGEAANPLDADPSGALRFFP
                     TSEEYKEHLTFLNKLYSKGLIQSDVFSNDSAKFNTLGKQGLVGAAAMQAPIASFGQEE
                     GEKYVALGPLKRSSGDEVPTWNPVGSGLASMGQFVLTDKSTHPIETARWIDYRYGDEG
                     SRLFFMGIEGESYEETGDGQYEFTDEITDNPDGLTVDEALKPYVTYLGGSYAGIVKEA
                     YFKGTESSPQAREGAEKIAPYRIEEVWPDFTYTSDEAAELSSLTVDLDKLRTEARDTF
                     INGKKSLKEWNNYLEDFDRIGLSRYMEIQQAAYDRYTG"
     gene            complement(1676..>2291)
                     /locus_tag="VK103_00945"
     CDS             complement(1676..>2291)
                     /locus_tag="VK103_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82074.1"
                     /translation="AAHTVTTRWVVGDRNVAVGTTMWREWRRHWSRAAPVSVLATALT
                     VSLAATWWFAGQQAPIPGAVARALSTVGLLLMAALLPHLAWVVERTDLTVARTVLAAL
                     AAGLSRPLLTVTLLVIGLGWPTALVLSGWPGLLPVLGVSVPMLAAAWCINRVFPAAEP
                     ATPGEGDDTGPASTDSAPHTPETPTRQGTPTVPVPSVSKGTEAP"
BASE COUNT          333 a          757 c          784 g          417 t
ORIGIN      
        1 gtgggaggat cgtggaccga gagcgcaggt ggcgacgatc aacccgtgta gcgatcgtaa
       61 gcggcctgct ggatctccat gtagcgcgac aggccgatgc ggtcgaagtc ctccaggtag
      121 ttgttccact ccttcaggct cttcttgccg ttgatgaagg tgtcgcgggc ctccgtgcgc
      181 agcttgtcca ggtccacggt cagcgagctc agctcggcgg cctcgtcgga ggtgtaggtg
      241 aagtccggcc acacctcctc gatcctgtac ggtgcgatct tctcggcgcc ctcacgtgcc
      301 tgcggactgc tctcggtgcc cttgaagtac gcctccttca cgatgccggc atagctgccg
      361 ccgaggtagg tgacgtacgg cttgagcgct tcgtcgaccg tcaggccgtc ggggttgtcg
      421 gtgatctcgt cggtgaactc gtactgaccg tcaccggtct cctcgtagct ctcgccctcg
      481 atgcccatga agaacagccg gctgccctcg tcgccgtacc ggtagtcgat ccaccgcgcg
      541 gtctcgatgg ggtgggtcga cttgtcggtg agcacgaact ggcccatgct cgccagcccg
      601 gacccgaccg ggttccaggt cggcacctcg tcgccggacg acctcttcag cgggccgagg
      661 gcgacgtact tctctccctc ctcctggccg aaggaggcga tcggcgcctg catggccgcg
      721 gcaccgacca ggccctgctt gccgagggtg ttgaacttcg ccgagtcatt gctgaagacg
      781 tcggactgga tcaggccctt ggagtacagc ttgttgagga acgtcaggtg ttccttgtac
      841 tcctcgctgg tcgggaagaa gcgcagcgcg cccgacggat cggcgtccaa cgggttggcg
      901 gcctcgccac gggtcccgat gccgaaggcg ccccagaaca tctggaccaa ccagccgatc
      961 gcgctgccat cggtgaaggg gatcgcggcc gggctgccct tcttgaccgg attcttgccc
     1021 ttgacctcgg tcaggaaggc ctcgaactcc tccagggtct ccggctccga catcccgaag
     1081 tcgtccaacc agtccttgcg gatccacagc ttctgctgca tcatcagcga gtcgaacgcc
     1141 gggtcgtaga tcgtcggcat cgagtagatg ttgccgtccg gcatcgtcat gcccttggcg
     1201 atgtcggggt acttgtccat cacgcccttc aggttgggca tgtactcgtc gatgagctcg
     1261 ttcagcgcga tgaaggtgcc ctgctcgccg tacttggcca ggtccaccgc cgacaccgcg
     1321 gtccggttca ggatcgccgg gtagtcaccg ctggccaggg ccaggttgcg cttctcggcg
     1381 gccccctccc acggcaccgg gccccagtcg accttgatgt tggtcaactc ctccaacttc
     1441 ttcaccgact tgacggtgtt ccagtcgtcg gcggtgctcg ggcggtgccg ggtcatcatg
     1501 tggagggtga tggactcctt gacgatcggc atcccggtct tgttgatgtt ggcgtccggg
     1561 ttgtcgatct tgtcctgggt ctcactgtcg ctcccggcac caccgcacga ggcgagcagc
     1621 ggggcggcaa cggcagcagc gccgaggccg agcgcggtcc tacgggtgat cggcttcatg
     1681 gtgcttcggt tcctttcgat acggatggca cgggaacggt cggtgttccc tgcctggtgg
     1741 gggtctcggg ggtgtgcggt gcagagtcgg tggatgccgg gccggtgtcg tcgccctcgc
     1801 cgggcgtggc gggctccgcg gcggggaaga cccggttgat gcaccaggcc gcggcgagca
     1861 tggggacgct gacgccgagg acgggcaaca gccctggcca gccactcaac accagcgcgg
     1921 tcggccagcc gaggccgatg acgagcagtg tgacggtgag cagcggccgg gacaatccgg
     1981 cggcgagggc ggcgagcacg gtgcgcgcca ccgtcaggtc cgtgcgctcc acgacccacg
     2041 ccaggtgggg cagcagggct gccatcaaca gcagcccgac ggtggacagc gcgcgggcga
     2101 ccgcccccgg gatcggcgcc tgctgaccgg cgaaccacca ggtggcggcc agcgacaccg
     2161 tgagggcggt cgccagcacg ctcaccgggg cggcccgcga ccagtgccga cgccactccc
     2221 gccacatggt ggtgcccacg gcgacgttcc ggtcgccgac gacccaccgg gtggtgacgg
     2281 tgtgcgcagc c
//
LOCUS       DATGUK010000062         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_72490,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000062 DATGUK010000000
VERSION     DATGUK010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72490"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2074
                     /locus_tag="VK103_00950"
     CDS             <1..2074
                     /locus_tag="VK103_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HLQ82075.1"
                     /translation="IAGQGFAMVTDTHQNLRRVERHGELSRIFEHIMDRDPAFVVNCG
                     DLADFGVADEFAGYRSTIPNGLADRVHQVPGNHESQWNTDGFEAYRRLLGPAHYSFDA
                     AGLHVIGLDPVVSQEWDFHFGAEVMQWLADDLADAPADTPIVIFSHFPLSNKWHYAMN
                     AEELYEAIEPYPVRLLLSGHTHSQSLLHFNGLTQLVGDSTADDPGYYWFDRQTDDDGD
                     RLVVTHVLMPPDGPVEEVAVTTVPLDEPGPGGSAGPVGASVEQAGGDTVRVDLQAPEA
                     ATVAYAQLWPQGAETKPFVELAATDPGRWSGEVDVSYLPPGEHRMALRTNDADGAWYD
                     GMQRFTLPSDTISLGWEHAFGERVQADLAVAGDLVVGASILGEVAALRVGTDAAEPVW
                     ERTLGPVYKGSRFVADDSLIAVPSADHHLYGLDAETGGTSWSRDLGAPVMSCLSSVTV
                     DDEEVLLAVAGSSLHCLGIDGSSRWSASIGGVSSGQVAGDGERVHIGAGNGYAYGFST
                     ADGSQLWRTKCTTQTSRYGALIHGPWASRMRLLDDGGVIATTYTELFCLEPDSGEVRW
                     SVKGFDHGSYTAPAVTPRGVLAVNGTPGTISLHDPATGETRWTADALPISNGAGLIPT
                     EDPDVWWMAGNTGLLVRIDLAEQQVEQVFQISTAWSNATGVLVDVGGDPVLVAAAKDG
                     RVRGVVGI"
BASE COUNT          369 a          805 c          770 g          331 t
ORIGIN      
        1 catcgccgga cagggattcg cgatggtcac cgacacccac cagaacctgc gccgggtgga
       61 gcgccatggc gagctcagcc gcatcttcga gcacatcatg gaccgcgatc cagcgttcgt
      121 ggtcaactgc ggcgacctgg ccgacttcgg ggtggccgac gagttcgccg gctaccgctc
      181 caccatcccg aacgggctgg ccgaccgggt ccaccaggtg ccgggaaacc acgagtcgca
      241 gtggaacacc gacggattcg aggcctaccg tcgactcctc ggcccggccc actacagctt
      301 cgacgccgcc ggcctgcacg tcatcgggct ggatccggtg gtgtcgcagg agtgggactt
      361 ccacttcggc gccgaggtga tgcagtggct cgccgacgac ctcgccgatg cgccggccga
      421 caccccgatc gtgatcttca gccacttccc gctcagcaac aagtggcact acgcgatgaa
      481 cgccgaggag ctctacgagg cgatcgagcc gtacccggtc cggttgctgc tgtccgggca
      541 cacccactcg cagtcgttgc tgcacttcaa cgggctgacc cagttggtcg gtgactccac
      601 ggccgacgat cccggctact actggttcga ccggcagacc gacgacgacg gtgatcggct
      661 cgtggtcacc cacgtgctga tgcccccgga cggtccggtc gaggaggtcg cggtcaccac
      721 cgtgccgctg gacgagccgg gcccgggtgg cagcgccgga ccggtcgggg cgagcgtgga
      781 gcaggccggc ggtgacaccg tacgggtcga tctgcaggcc cccgaggccg ccaccgtcgc
      841 ctacgcacaa ctgtggccgc agggcgccga gaccaagccg ttcgtggagc tggccgccac
      901 cgatccgggc cgctggagcg gtgaggtcga tgtgtcgtac ctgccgccgg gtgagcaccg
      961 gatggcgctg cgcaccaacg acgccgacgg cgcctggtac gacggcatgc agcggttcac
     1021 gctgccgtcc gacaccatct ccctcggatg ggagcacgcc ttcggtgagc gggtgcaggc
     1081 cgacctcgcg gtcgccggcg acctcgtcgt cggagccagc attctgggtg aggtcgccgc
     1141 gctgcgggtc ggcaccgatg cggccgagcc ggtctgggaa cgcacgctcg gcccggtgta
     1201 caagggatcc cgattcgtgg ccgacgacag cctcatcgcc gtaccgtcgg ccgaccacca
     1261 cctgtacggc ctcgacgccg agaccggtgg gacgagctgg tcgcgggacc tcggcgcacc
     1321 ggtgatgagc tgcctgtcca gcgtcaccgt cgacgacgaa gaagtcctgc tggccgtggc
     1381 cggcagcagc ctgcactgcc tcggcatcga cggcagcagc cgctggtcgg catcgatcgg
     1441 cggcgtcagc tccggccagg tcgccgggga cggcgaacga gtccacatcg gtgccggcaa
     1501 cggatacgcc tacggtttct ccaccgccga cggcagccag ctgtggcgca ccaaatgcac
     1561 cacccagacc tcccgctacg gcgccctcat ccacgggccg tgggcgtcgc ggatgcggct
     1621 gctcgacgac ggcggcgtca tcgccaccac ctacaccgaa ctgttctgcc tggagcccga
     1681 cagcggcgag gtgcgctggt cggtcaaggg cttcgaccac ggcagctaca ccgccccggc
     1741 cgtcacgccg cgcggcgtcc tcgccgtcaa cggcaccccg ggcaccatct cgttgcacga
     1801 cccggcgacc ggcgagacca ggtggaccgc tgatgcgctg ccgatcagca acggtgccgg
     1861 cctcatcccg accgaggacc ccgacgtgtg gtggatggcc ggcaacaccg gcctgctggt
     1921 ccgcatcgat ctggccgagc agcaggtgga gcaggtgttc cagatcagca ccgcgtggtc
     1981 caacgccacc ggcgtgctcg tcgacgtcgg tggggacccg gtgctggtgg cagccgccaa
     2041 ggacggacgg gtccgcgggg tcgtcggcat ctgaggccac gatcgacagg tcgaccggag
     2101 gcatccctac taatgcctca aaataccttg acgcattagt ttccattgtg gtggtgtggc
     2161 tcgggtccgc tggtccaggc ggatgaccaa cgcactccta ccgggggagg gagcgtgaca
     2221 cgtcaacact gatcggagca ctcaccatgg acgcaaacct cgcccggaca cgagc
//
LOCUS       DATGUK010000063         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1468312,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000063 DATGUK010000000
VERSION     DATGUK010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468312"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VK103_00955"
     CDS             complement(<1..384)
                     /locus_tag="VK103_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82076.1"
                     /translation="MTDPLPAPGPSTTGSSAAGANLPRLTSDELTLRYLPGRGGVITS
                     LTRNRDDVPVLWQAPWGVLPVGASRPPATASVAAREGWAGGWRTLFPNSGRAVTLYGA
                     DQGYDGEACLAPYRVDQADDRAVTLR"
     gene            complement(450..929)
                     /locus_tag="VK103_00960"
     CDS             complement(450..929)
                     /locus_tag="VK103_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82077.1"
                     /translation="MFVIGGVILAAVLALVIGGIATWRADVAGREASPTPAPSRTGSP
                     LPPNVRPFVTDTCKDGLFEVLEHERSGSGDLYIEVRISCHDGRYRAQDDAVAIFDKFS
                     QSYLNSPPSDRPTIGNTVVDAGNPVQGWARFTGVPPGEVTVLLLGYGHTTTAVPIDT"
     gene            1026..1412
                     /locus_tag="VK103_00965"
     CDS             1026..1412
                     /locus_tag="VK103_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82078.1"
                     /translation="MRAGSHVGRAAVQVRALPVLTVRRLAGGRLLAVLTVLLVLTVLA
                     VLTVLAVGLLARRRPLTGRWLLVERWARRASLTGRPAGMVRLSPLVAHSSKGTCPQAG
                     CGSDGPTTVVHRQTIMAAIPAPGGTV"
     gene            1418..2461
                     /locus_tag="VK103_00970"
     CDS             1418..2461
                     /locus_tag="VK103_00970"
                     /inference="COORDINATES: protein motif:HMM:NF025200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HLQ82079.1"
                     /translation="MTSPEPMPEPDPIHGGSSHDADRAPVIPLHGPPRVRVGGADDML
                     ALIPYLLGFRPEQSLVVMVLRQHRVVLTARMDLPRSMEECEQVADHFARVAVRHDAED
                     CLVAVYSTEWEHAHLVLMTLALSDDLPVVTALHCDDDQYRMLAPDGTMEGPYGFDPRA
                     SAAAAAAVVAGLSVAPDRESLAARIMAGPRELTDQVVAAAEDVDLPEEQDERRDLMDR
                     TVSRCLPEKDAVAELSDSDCALLGLLCLDLEVRDVAWLRMERETASRHLALWIEVSRR
                     VPPVLTAAPVCLAGMASWLDGDGALAWCCAERAEEEHPGYGLAGLLGEILAATLHPGA
                     WHELAEEMRTLYR"
     gene            2471..>3022
                     /locus_tag="VK103_00975"
     CDS             2471..>3022
                     /locus_tag="VK103_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLQ82080.1"
                     /translation="MARADIAPVPAGTPFLVVGEALIDLVPDGDGSTFDSPWQAQTAG
                     GPLNTAVALARLGRTVDFGAPLARDAFGRQMLAHLDANGVGRDRRPSGEGATALAVVS
                     LDEHGKASYHFHRHGTATFDFRADRMPKPREGEWLHVASLVLVVEPGADELLAWVRGT
                     DAPLSIDVNVRTIVEPDPVAYWRR"
BASE COUNT          408 a         1018 c         1145 g          451 t
ORIGIN      
        1 gcgcagggtg accgcccgat cgtccgcctg gtccacccgg tacggggcga ggcaggcctc
       61 cccgtcgtag ccctggtccg ccccgtacag cgtgaccgct cgacccgagt tcgggaacaa
      121 ggtgcgccaa ccgccggccc atccctcccg ggccgccacc gacgcagtcg cggggggccg
      181 gctggctccg accggcaaca ccccccacgg cgcctgccac agcacaggga cgtcgtcgcg
      241 gttgcgggtg agcgaggtga tcacgccgcc ccgtccggga aggtagcgga gagtgagctc
      301 atcgctggtc aggcggggca ggttggctcc tgctgccgag gaccctgtcg tcgaaggtcc
      361 gggggcgggc aggggatcag tcatcgaggc gggtccttcc gagcaggtgt gcgcaacggc
      421 cgggggccgg tggcggtggc gggtcgacct caggtgtcga tcggcacggc ggtggtggtg
      481 tgaccgtacc cgagcagcag gaccgtgacc tcaccgggcg gcaccccggt gaaacgcgcc
      541 caaccctgca ccgggttgcc ggcgtccacg acggtgttgc cgatggtggg ccggtcggac
      601 ggaggcgagt tcaggtacga ctggctgaac ttgtcgaaga tcgcgaccgc atcgtcctgc
      661 gcccggtagc ggccgtcgtg gcagctgatc cggacttcga tgtagaggtc gccgctgccg
      721 ctgcgctcgt gctccaacac ctcgaacagg ccgtccttgc aggtgtcggt gacgaacgga
      781 cgcacgttcg ggggcagggg gctgccggtg cggctcgggg ccggcgtggg gctcgcctcc
      841 cgcccggcga cgtccgcgcg ccaagtggcg atgccgccga tcaccagtgc gagcacggca
      901 gccaggatga ccccgccgat cacgaacatc gggactttcg acggcggcgg tggcccgggc
      961 cgggtcaccg gggtacgcgg gtccagccca ctgccgaacg gtcgactcgg gtcgtggcgc
     1021 gcgccgtgcg tgccggatcc catgtcggcc gggccgcggt gcaggtcagg gcgctgcccg
     1081 tactgaccgt acggcggctg gccggcgggc ggctgctggc cgtactgacc gtgttgctcg
     1141 tactgaccgt gttggccgta ctgaccgtgt tggccgtagg gctgctggcc cgcaggcggc
     1201 cgctgaccgg ccggtggctg ctggttgaac ggtgggcccg acgggcgtcc ctgaccggac
     1261 gcccggccgg gatggtccgg ctctccccac tggttgctca ctcgtccaaa ggtacctgtc
     1321 cacaggccgg gtgcggttct gacggtccca cgacagttgt gcacaggcag acgatcatgg
     1381 cggccattcc cgccccggga ggcacagtgt gaggggcgtg accagtcctg aaccgatgcc
     1441 cgagcccgat ccgatccacg gggggtcgtc gcacgacgcc gaccgtgctc ccgtcatccc
     1501 gctgcacggc ccaccccggg tgcgtgtcgg cggcgccgac gacatgttgg cactcatccc
     1561 gtacctgctg ggcttccgcc cggagcagtc gctggtggtg atggtcctgc ggcagcaccg
     1621 tgtggtgctc accgcacgca tggacctgcc gcggagcatg gaggagtgcg aacaggtcgc
     1681 cgaccacttc gcgcgggtcg cggtgcgcca cgacgcggag gactgcctgg tcgcggtcta
     1741 ctccaccgag tgggagcacg cccacctggt gctgatgaca ctggcactgt cggacgacct
     1801 gccggtggtc acggccctgc attgtgacga cgaccagtac cggatgctgg cccctgacgg
     1861 gacgatggag ggcccgtacg gcttcgaccc ccgcgcgagc gccgcggcag ccgcagcagt
     1921 ggtcgccggc ctgtccgtgg caccggaccg tgagtcgttg gcggcccgga tcatggccgg
     1981 cccgcgcgag ctgaccgatc aggtggtcgc cgcggccgag gacgttgacc tgccggagga
     2041 gcaggacgaa cgacgtgacc tgatggaccg caccgtcagc cgttgcctgc cggagaagga
     2101 cgcggtcgca gagctgtccg acagcgactg tgcactgctc gggttgctct gcctcgacct
     2161 ggaggtccgt gacgtcgcgt ggctacggat ggagcgggag accgcgagca ggcacctggc
     2221 gttgtggatc gaggtgtccc gtcgggtacc gccggtgctg accgccgcgc cggtgtgcct
     2281 ggcggggatg gcgtcctggc tcgacggtga cggtgcgttg gcgtggtgct gtgccgaacg
     2341 ggccgaggag gaacaccccg gctacgggtt ggcaggcctg ttgggcgaga tcctcgccgc
     2401 gacgctccac cccggtgcct ggcacgaact ggccgaggag atgcgcacgc tgtatcgcta
     2461 gtctcacggg atggctcgag ctgacattgc accggtgcct gccgggacac cgttcctggt
     2521 ggtgggcgag gcgctgatcg acctggtccc ggacggggac ggctccacgt tcgactcacc
     2581 gtggcaggcg cagaccgccg gaggaccgtt gaacaccgcg gtcgcgctgg cccggctggg
     2641 acgcaccgtc gacttcggcg caccgctggc ccgggacgcc ttcggccggc agatgctggc
     2701 gcacctggac gcgaacgggg tcggccggga ccggcggccg tcgggggaag gtgcgaccgc
     2761 actggccgtg gtgtcgctgg acgagcacgg caaggcgtcg taccacttcc accgccacgg
     2821 caccgcgacg ttcgacttcc gcgcggaccg gatgccgaag ccgcgggagg gggagtggct
     2881 gcacgtcgcc tcactggtgc tggtggtcga gccgggtgcc gacgagctgc tggcctgggt
     2941 acggggcacc gacgcgcccc tgtcgatcga tgtcaacgtg cgcacgatcg tggagccgga
     3001 cccggtcgcc tactggcgcc gg
//
LOCUS       DATGUK010000064         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1355892,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000064 DATGUK010000000
VERSION     DATGUK010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355892"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VK103_00980"
     CDS             complement(<1..504)
                     /locus_tag="VK103_00980"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLQ82081.1"
                     /translation="MATGPGTAAQPEVTRVILVRHAQHDPGDRFRQHSCTGLTDHGRD
                     QAERLARRLRDHHLGIDTVVTSRAARTIETGAAVAEALGIVPAAPTCDLCEMHPGEAE
                     GLTVAEMQQRWGRNYAAVPGAQYFPDWLPGAQRRLRVLAEQHRGRTVLAVTHNAVVRV
                     SFLAFAGM"
     gene            complement(506..1024)
                     /locus_tag="VK103_00985"
     CDS             complement(506..1024)
                     /locus_tag="VK103_00985"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HLQ82082.1"
                     /translation="MGPVNTQQQSAPRPDATEGLHIVFVCHGNICRSPMAERMAEQAA
                     DEAGLEHVRFSSAGVSSEEAGNPIDPRAVRKLRELGCRTGDHRAHRITADEARGADLI
                     ICAEQRHADRVVGLDAELAPRVMLLTDFVPGAEPGSGVPDPWYGDASGFDDTADTIAA
                     ALPAILDRTRSS"
     gene            1088..1471
                     /locus_tag="VK103_00990"
     CDS             1088..1471
                     /locus_tag="VK103_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017946299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HLQ82083.1"
                     /translation="MRMLARLVLNAIALAVATWLVPGISLDVEDRIRAAVVLLVVALV
                     FGLVNAWVKPIVQFVSIVAVVLTLGLFLLVINTLMLWLTSWVSGLLDLGWHVDGFWSA
                     LFGALIVSIVSVLLGFIVPEKKHDR"
     gene            complement(1554..>2024)
                     /locus_tag="VK103_00995"
     CDS             complement(1554..>2024)
                     /locus_tag="VK103_00995"
                     /inference="COORDINATES: protein motif:HMM:NF037405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HLQ82084.1"
                     /translation="AGRCTTAEYFVAILDSLLRQGHRPADLVHMLRHCPVRVRRLVAH
                     TDLADSGTESLVRFRLRAAGIRVRPQVRIHGVGRVDLLVGSRLVLEVDSRAHHSAPDA
                     YVTDRDRDLALARLGYDRLRVTYEHVLHRWDEAFAAIMTIIRRGDHRRTPRPIT"
BASE COUNT          257 a          686 c          758 g          323 t
ORIGIN      
        1 catcccggcg aaggcgagga aggagacacg gacgacagcg ttgtgggtga cggccagcac
       61 ggtccggccc cgatgctgct cggcgagcac ccgcagccgt cgttgcgctc ccggcagcca
      121 gtccgggaag tactgggctc ccggcaccgc ggcgtagttg cggccccagc gctgttgcat
      181 ctcggcgacc gtcaacccct cggcctcgcc gggatgcatc tcgcacaggt cgcaggttgg
      241 cgcggccggc acgatcccca gtgcctcggc caccgcagcc ccggtctcga tcgtccgggc
      301 cgctcgcgag gtcacgacgg tgtcgatccc gaggtgatgg tcacgcagcc ggcgcgccag
      361 tcgctccgcc tggtcccggc cgtggtcggt cagaccggtg cagctgtgct gccggaaccg
      421 gtcgcccggg tcatgctggg cgtggcgcac caggatgacc ctcgtcacct ccggttgggc
      481 ggccgtcccc ggtccggtgg ccacctcagc tgctccgggt ccggtcgagg atggccggga
      541 gtgcggcggc gatggtgtcg gcggtgtcgt cgaaaccgga ggcgtcgccg taccacgggt
      601 cgggcacccc gctgccgggc tcggcaccgg gcacgaagtc ggtgagcagc atgacccgtg
      661 gggccagctc cgcgtccagg ccgaccaccc ggtcggcgtg ccgctgctcg gcacagatga
      721 tcaggtcggc gccccgggcc tcgtcggcgg tgatgcggtg cgcccggtgg tcgccggtgc
      781 ggcagccgag ctcgcgcagc ttgcggaccg cgcgcgggtc gatcgggttg ccggcctcct
      841 ccgaggagac tccggcgctg ctgaaccgga cgtgctcgag cccggcctcg tcggcggcct
      901 gctcggccat ccgctcggcc atcggggacc ggcagatgtt gccgtgacag acgaagacga
      961 tgtggagccc ctcggtggcg tcggggcgtg gcgctgactg ctgctgggtg ttcacgggcc
     1021 ccatcgtgcc agtcacccgt tcccggccgg gtggggctgg ggtgggactg gcacgatgga
     1081 ggacgtgatg aggatgcttg cccggctggt gctcaacgcc atcgcgctcg cggtggcgac
     1141 gtggctggtc cccgggatca gcctggacgt ggaggaccgg atccgcgccg cggtcgtgct
     1201 gctcgtcgtc gcgctggtgt tcgggctggt caacgcctgg gtgaagccga tcgtgcagtt
     1261 cgtcagcatc gtcgcggtgg tgctgacgct cggactgttc ctgctggtca tcaacaccct
     1321 gatgttgtgg ctcacctcgt gggtgagcgg cctgctcgat ctcgggtggc acgtcgacgg
     1381 gttctggtcg gcactgttcg gcgcgctgat cgtgtcgatc gtcagcgtgc tgctcggttt
     1441 catcgttccc gagaagaagc acgaccgctg atgcgtaccg tcgctcagtc ccagcgggga
     1501 ccggatggca gaagcgcctc gtcagcgccg atcttcctga gcgacggcac ggatcacgtg
     1561 atcgggcggg gggttcgccg gtggtcgccg cgccggatga tggtcatgat cgcggcgaag
     1621 gcctcgtccc agcggtgcag gacgtgctcg taggtcaccc gtagccggtc gtagccgagc
     1681 cgggccagcg ccaggtcacg gtcgcggtcg gtgacgtagg cgtcgggagc gctgtgatgg
     1741 gcgcggctgt ccacctccag gacgagccgg gacccgacca gcagatcaac ccgtcccacc
     1801 ccgtggatcc gcacctgcgg acgcacccgg atccccgccg ctcgcagtcg gaaccggacc
     1861 aggctctcgg tgccggagtc ggcgagatcg gtgtgggcga ccagccggcg cacccgaacc
     1921 gggcagtggc gcagcatgtg caccagatcc gccgggcggt gcccttggcg cagcagggag
     1981 tccaggatcg ccacgaagta ctccgccgtg gtgcaccggc cggc
//
LOCUS       DATGUK010000065         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_73760,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000065 DATGUK010000000
VERSION     DATGUK010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73760"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(531..1565)
                     /locus_tag="VK103_01000"
     CDS             complement(531..1565)
                     /locus_tag="VK103_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLQ82085.1"
                     /translation="MAGGTRRPTQRDVAELAGVSMATVSHVVSGRRDRRNPTSPDVAE
                     RVRSAMAELGYRPHRAGRVLQSRRTRLIAVAAYTPLNPWALTAIAQIEEVADRHGLGV
                     VIVRYGSEAATTDRAEELLNDGIADATAVLYGPAFGEERARRLGASGLPTLVFGLPRP
                     APGVDVLVQHEERAIGDAARYLVETGSDRLAFISRSGPTLPDQRGDAFVTAARGAGLP
                     TDRVTLVRSPQDQYATDVRVLQRAVELLRLPRRTRPDAIMVPSDRGAINVMWAAIQEG
                     LRVPDDVRIIGAGNIDQCLQVSPHLTTVGCVDVDYRPAIERLIQRIGRPDRDNRAIEV
                     PWELIIRGTT"
     gene            1692..>2736
                     /locus_tag="VK103_01005"
     CDS             1692..>2736
                     /locus_tag="VK103_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLQ82086.1"
                     /translation="MGTAHQRIGFTRRGVLAAAATGGLAALAGCQVDTGGTTGGGSGG
                     GDDPTKITFPDYGTEITSENATLRWVDSGDLKATFNGPAMDAFTEKYPNIKTKYDGGG
                     WALVNKVVPLGIRNGNAPDVFAKPQGMPYATMVTEGWIRPLDDLIPDFEEWKAAFPDT
                     AFIPGLHTFDDKTYSWPQSSNRRLTHMCIWDKKNLAEAGYDDPGAQIQTWDELHAALT
                     KVVKNDHVGVMVGLEDMQGLINSLAASIGWIGVEGMDYRTGKFIWDAPEILEAAEFLQ
                     KLVTDKLIVPGFLTLKQADARAQMAAGDAAVVISGPWDIPRWKEQSPDWEYVMEQLPG
                     RDGLDYILPKQLHG"
BASE COUNT          437 a          917 c          931 g          451 t
ORIGIN      
        1 ttgaggcatc gctactggag gacttcgttg agtccctgta gattcccgtg actgcatggt
       61 tacttcgagg ctctgtgact gccatttgta tacgtccaaa cagtcatccg gtgtacgtcg
      121 aagcgtcgac agatcgagac cacgctctcg ccattcgctc gggcagttct aatagcgtcc
      181 acttggctgt ccgttagcgg tgttctagct cgtctcccag ggttcgcgac gacccccggc
      241 tcgtcatcac cggcaccgtc cggctgggca cccagaccga gtttcgcctg cttccacgcg
      301 gagctaagcc gttcgaaccg ctgccaggtg ttcggtttga gtccacccgc gtccaccctt
      361 gtgatgagtc gggacatgtg tccctgatga gtcgccacat gtgtcacgaa gggcccggcc
      421 atcggccggg ctcttttgtt gtgtttgggg tgcgggccga tggcggccgg gaaggaggtg
      481 acggcgcggt cggccggacg gctggtcctg ccggcccccg ggtacgaggc tcacgtggtg
      541 ccgcggatga tcagctccca ggggacctcg atcgcccggt tgtccctgtc cggcctgccg
      601 atccgctgga tgaggcgctc gatcgcgggc cggtagtcga cgtcgacgca gcccaccgtg
      661 gtgaggtgcg ggctcacctg caggcactgg tcgatgttgc cggcgccgat gatgcggacg
      721 tcgtcgggga cccgcaggcc ctcctggatg gcggcccaca tcacgttgat cgcaccacgg
      781 tccgacggca ccatgatcgc gtcggggcgg gtgcggcggg ggaggcgcag cagctcgacc
      841 gcccgctgca gcacccggac gtccgtggcg tactggtcct gggggctgcg gaccagcgtc
      901 acccgatctg tgggcagtcc ggccccgcgg gccgcggtca cgaacgcgtc gccacgctgg
      961 tcgggcagcg tcgggccgga acgggagatg aaagcgaggc ggtccgatcc ggtctcgacc
     1021 aggtagcgtg cggcgtcgcc gatcgcccgt tcctcgtgct ggaccagtac gtccaccccg
     1081 ggggcgggtc gcggcagccc gaacaccagc gtcgggagcc cggaggcccc gagccggcgt
     1141 gcccgctcct cgccgaaggc cggcccgtac agcaccgccg tggcatcggc gatcccgtcg
     1201 ttcagcagtt cctcggcccg gtcggtggtg gcggcctcac tgccgtaccg gacgatgacc
     1261 acgccgagcc cgtgccggtc ggccacctct tcgatctgcg cgatggcggt gagcgcccag
     1321 gggttcagcg gggtgtaggc cgcgaccgcg atcagccggg tccgccggct ctgcagcacc
     1381 cgcccggccc ggtgcggtcg atagccgagc tcggccatgg ccgagcggac ccgttcggcg
     1441 acgtcgggcg aggtggggtt gcggcggtcg cgtcgcccgc tcaccacgtg tgagacggtc
     1501 gccatcgaca ccccggccag ctccgcgacg tcccgctgag tggggcgacg ggtccctccg
     1561 gccatgggtt gccctccctg tctcgatggc gaccgtcatt gtcccagacc cttgacaatg
     1621 gtctgtccag accgtcatgg tgatacgcat aaccagcgtc cgtcaatcac cgcacgtcaa
     1681 gggagacatc catggggaca gcgcatcagc ggatcggatt cacccgccgc ggagtgctcg
     1741 cagctgcagc caccggcggg ctcgccgcac ttgccggctg ccaggtcgac accggtggga
     1801 ccacgggtgg tggctccggg ggcggtgatg acccgaccaa gatcaccttc cctgattacg
     1861 gcaccgagat caccagcgag aatgccacgc tgcgctgggt ggactcgggc gacctgaagg
     1921 ccaccttcaa cgggccggcg atggatgcct tcaccgagaa gtaccccaac atcaagacca
     1981 agtacgacgg tggcggctgg gccctggtga acaaggtcgt gccactcggc atccgcaacg
     2041 gcaacgcacc agatgtcttc gccaagccgc agggcatgcc gtacgccacg atggtgactg
     2101 agggctggat ccgcccgctg gacgatctga tcccggactt cgaggagtgg aaggctgcct
     2161 tccctgacac cgccttcatc ccggggctgc acaccttcga cgacaagacc tactcctggc
     2221 cccagagctc caaccggcgg ttgacccaca tgtgcatctg ggacaagaag aacctcgccg
     2281 aggctggcta cgacgatccc ggcgcgcaga tccagacctg ggacgagctc cacgccgccc
     2341 tgaccaaggt ggtcaagaac gaccatgtcg gggtgatggt cgggttggag gacatgcagg
     2401 gcctgatcaa cagccttgcc gcgtccatcg gttggatcgg cgtcgaaggc atggactacc
     2461 gcaccgggaa gttcatctgg gacgccccgg agatcctgga agccgccgag ttcctgcaga
     2521 agctggtcac cgacaagctg atcgtgcccg gattcctcac gctgaaacag gccgacgcgc
     2581 gagcccagat ggccgcgggc gacgcggcgg tggtcatctc cgggccgtgg gacatcccgc
     2641 ggtggaagga acagtccccg gactgggagt acgtgatgga gcagttgccc ggccgggacg
     2701 gactcgacta catcctgccc aagcagttgc acggtg
//
LOCUS       DATGUK010000066         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_422174,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000066 DATGUK010000000
VERSION     DATGUK010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422174"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="VK103_01010"
     CDS             complement(<1..1351)
                     /locus_tag="VK103_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLQ82087.1"
                     /translation="MSGNDARSHRTTHPAVPPVPHRPTGEVRRRALLGGTAGGALLAG
                     STLGRDRAAARPRHHDTPAAEPSQSPAAARRQRFAFVTDVHINDLEPERVTHFRRVTS
                     AILADDPELVISGGDMTDWGRDTECEAYWSAVPDALRSRMHHVPGNHELKYDWSDYAN
                     YERWFGERNVAVDVGGLHFLFVDTSVCQLDPAYLRRDTMAWLRDTLEAAGSEPSIIVT
                     HHPPMGDQFYFLPNQDELHRVIEPYPVRGILCGHTHAQRVLRTNGLTLLVGRTAKKHA
                     GFYRLERTTDDTDDRIEISYVEAPGPGTDGPGPATPVTTIDLTAPHGPGGDLAPTRTR
                     VRCSTDGTLAVRARHPREAPVADVAATVYNPDVFRGNAPDTWTPLTGSDDGALHTGQL
                     TLDGHAPGNHRVIIRSRDAAGDQWREIVDVQTPAKAFDTLWEHRMPESVQGSLAQHDG
                     "
     gene            1448..>2081
                     /locus_tag="VK103_01015"
     CDS             1448..>2081
                     /locus_tag="VK103_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HLQ82088.1"
                     /translation="MALFGRRKQTADPETGTDDHAPAPAEDDTPVAPPVGPGGMRSVD
                     DHRDFLLSLVDELPPFGQQILDALDLTLCEDVVSEVDLPAFDNSAMDGYAVQVADLVG
                     ARDPDPVVLAVVGEVAAGQTAPTPLSPGTAMKVMTGAPIPPGAEAVVPYEDTDRGADD
                     VLVYREAATGQHIRRRGEDLSSGTQVFSTGDRLGPGSIGLLAAIGHDKAMV"
BASE COUNT          263 a          691 c          802 g          325 t
ORIGIN      
        1 ggccgtcgtg ctgggcgagg gatccctgca ccgactccgg catccggtgc tcccacagcg
       61 tgtcgaaggc cttggccgga gtctgcacgt cgacgatctc gcgccactgg tcacccgcgg
      121 cgtcccggct gcggatgatc acccgatggt tccccggggc gtggccgtcc agcgtcagct
      181 gcccggtgtg gagcgcgccg tcgtcgctgc cggtcagcgg agtccaggtg tcgggggcgt
      241 tgccccggaa cacgtccggg ttgtagacgg tggccgcgac gtcggcgacc ggggcctccc
      301 gggggtgacg ggcccggacc gccagcgtgc cgtcggtgct gcagcggacc cgggtacggg
      361 tcggtgcgag gtcaccgccc ggaccgtgcg gggcggtcag gtcgatggtg gtgaccgggg
      421 tggccgggcc cggtccgtcc gtaccgggcc cgggggcctc cacgtacgag atctcgatcc
      481 ggtcgtcggt gtcgtcggtg gtgcgctcca ggcggtagaa gccggcatgc ttcttggccg
      541 tccggccgac cagcagcgtc agcccgttgg tccgcagcac ccgctgcgca tgggtgtggc
      601 cgcagaggat gccgcgcacc gggtacggct cgatcacccg gtgcagctcg tcctggttgg
      661 gaaggaagta gaactggtcg cccatcgggg ggtggtgggt cacgatgatc gacggctccg
      721 acccggcggc ctccagggtg tcgcggagcc acgccatcgt gtcgcggcgc aggtacgccg
      781 ggtcgagttg gcagacgctg gtgtcgacga acaggaagtg caggccgccg acgtcgaccg
      841 cgacgttgcg ctcaccgaac cagcgctcgt agttcgcgta gtcggaccag tcgtacttca
      901 gctcgtggtt gccgggcaca tggtgcatcc gggaacgcag ggcgtcgggg acggcactcc
      961 agtaggcctc gcactcggtg tcgcgccccc agtcggtcat gtcgccgcca ctgatcacca
     1021 gctcggggtc atcggcgagg atggcgctgg tcacccgccg gaagtgggtg acccgttccg
     1081 gctcgaggtc gttgatgtgc acgtcggtga cgaaggcgaa gcgctgccgt cgcgcagcgg
     1141 ccggtgactg actcggttcg gcggcggggg tgtcgtggtg gcgtggtcgg gcggccgcgc
     1201 ggtcgcggcc gagggtgctc cccgcgagca gcgcaccgcc ggcggtgccg cccagcagtg
     1261 cccggcgacg cacctccccg gtgggtcggt gcggtactgg cgggacggct gggtgggtgg
     1321 tgcggtgcga acgggcgtca ttgcctgaca tgcgggtcct cccggcggga atggtgaccc
     1381 gcccatggta gaccgatcta ctcgcggctc cgtcggagat cggttcggag agactaaggt
     1441 gtcgcccatg gcgttgtttg gacggcgaaa gcagaccgcg gatcccgaga ccgggaccga
     1501 cgatcatgcc cctgcgcccg cggaggacga cacgccggtc gcgccgcccg tcggccccgg
     1561 cggtatgcgg tcggtggacg accaccgcga cttcctgctc tcgctggtcg atgagctgcc
     1621 cccgttcggg cagcagatcc tcgacgccct ggacctcacg ctctgcgagg acgtggtgag
     1681 cgaggtggac ctgccggcgt tcgacaactc cgcgatggac gggtatgcgg tgcaggtggc
     1741 cgacctggtc ggtgcccgcg accccgatcc ggtcgtgctg gccgtcgtcg gcgaggtcgc
     1801 tgccggtcag accgccccga cgccgttgtc gcccgggacc gccatgaagg tgatgaccgg
     1861 cgccccgatc ccgcccggcg ccgaggccgt ggtcccgtac gaggacaccg accggggtgc
     1921 cgatgacgtg ttggtctacc gggaggcagc gaccgggcag cacatccgcc ggcggggcga
     1981 ggatctgtcg tcggggaccc aggtgttctc caccggcgat cggctgggac ccggctcgat
     2041 cgggctgctc gccgcgatcg gccacgacaa ggccatggtc c
//
LOCUS       DATGUK010000067         5001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_540774,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000067 DATGUK010000000
VERSION     DATGUK010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540774"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VK103_01020"
     CDS             complement(<1..451)
                     /locus_tag="VK103_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82089.1"
                     /translation="MSTPGPVHRSSGAAAPHTLDRHRTLDRHRTAPAPGPAPARTTTW
                     TQRYRRRLFVTDTVVVVLCMFGAQGLRDQLVSGAWWESWEHTSLSLLVSLGWLVSLHA
                     YGSRSARVIGVGSAEFHRIVGASIRVFGLFAIWALLARNDLGRGYLLV"
     gene            complement(451..1389)
                     /locus_tag="VK103_01025"
     CDS             complement(451..1389)
                     /locus_tag="VK103_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLQ82090.1"
                     /translation="MSIAEPIRQHEPIVPRVLPFIPAQPSTRASRRFPTPDQPGTTDD
                     VVGLSIGDLDLFRTRLGPLTRLQRSPAGQQCLLLARASEPILVSARGRRLAVSPQQLL
                     LVTNTADHVASASSRRATVTGVILPVSLLSEFAVQTPRRSLEVHPGSTLAPPMWQFID
                     SLLDQPLPWQSLSAYFAEKLLHEMIGSVLLETAAAPGVPFADRSLVERARHHITAYAR
                     DPRLSPVMVARSLGVPLRRLQRRFAEVESSLAAEIRSARATAAIAVLRSPEGCVLDIA
                     TVAKVSGYPSDVALRRSFAALGLEPPSHYRRTQKQA"
     gene            complement(1386..2423)
                     /locus_tag="VK103_01030"
     CDS             complement(1386..2423)
                     /locus_tag="VK103_01030"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HLQ82091.1"
                     /translation="MNAAVLVLVVGALALVLAVSGVAKLADRRRSHRALHDLQLPGSL
                     STQAFAVSWAILEVAVAVSLVVLPGRWAAAAAAVATAMMIGHLLVVARAARFAEPVHC
                     HCFGALDREPVTARTVVRTGGFLALAVVSLLGLVTLTGSSAAAGRSIATITPSLRGAD
                     LGLLQLGLLVAGAGAVVLLGRSTGQTRRGRSDDHQPGPVPDPATEGPMLGEHKLPDIF
                     GASPDVRTFTGEQRSVLVLLSTSCAACRSLAEQLPGLRSELAGLGVQLRAVTPLPAAE
                     ALGSFPLLGSFLHHDVDGSLTERLGVTTYPAAVAVGRDGPGSLDDGPALGSHAVTVLI
                     DNIRTAEGVRA"
     gene            complement(2611..3609)
                     /locus_tag="VK103_01035"
     CDS             complement(2611..3609)
                     /locus_tag="VK103_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82092.1"
                     /translation="MSSPNPGLSPDPSGVSRRTVVKGAAWTVPAVAVASAVPAQAASP
                     TGNNLAVSVSDCSLVTIRVGGENPGFTYTVTEGTIPAGTQITITSGGTVAISAGNFVF
                     DGLQLNLLGIGNGVATFETQQDLTIGQSIRIGLTGFTLNVIGTYTTTIITPDDVQTDD
                     IGIMNTGGVGLGSLGVFICFDAAGGGEEVPDLDFDVIVDYGWCETVGLGPAASFEIDA
                     SGGTIPAGTRFLLYSSAPLSLGIGNWTTGGGTALSLDLLGTGTQVAEFETPRDIAPGD
                     PLTVEYQGIQLNVASTFYLAYLGNDNDPANNATGMKVTGVTVPIFGTVANCERLTA"
     gene            3864..>5001
                     /locus_tag="VK103_01040"
     CDS             3864..>5001
                     /locus_tag="VK103_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82093.1"
                     /translation="MSSTDWSGRHHPTSKEDDVHSFTVRVLGRAIGVDCDPTTVPDDV
                     ADELSRQWEQCLTDADPDDRVWVSLDEAGPPPDHRYFVRGSDPRVAAHRLSDVVVGTA
                     MAALVPPEGRDRPLAQPPSVLGVHAAAVVRDRRAVLLVGGTHRDEAMRALAPHYDYLS
                     DSAVAVDLDRGTVLGAATPLTTFPEPELVSRTEVPLRDAGLRPVLEPVPLAAVVVLEP
                     TASAGAAGWTPLSIPVATARLAPAVPILPALPQPMQDLARVASLTRGAVSVSGAAPEL
                     PALLDEVLAVEMAAELVIPVEPGPGPDTTPRPGDVRRHRMTDGISDGSTLAFWTTEHT
                     AVAVGGIAPHLWDAARQWIGPDELTDRVVQAVGAPEGADPRALVD"
BASE COUNT          743 a         1671 c         1881 g          706 t
ORIGIN      
        1 cgaccagcag gtagccgcga cccagatcgt tgcgggccag cagcgcccag atcgcgaaca
       61 gcccgaacac ccggatcgag gcacccacga tccggtggaa ctccgcgctg ccgacgccga
      121 tcacccgcgc gctgcgcgac ccgtacgcgt gcaggctgac cagccatccc aggctcacca
      181 acaggctcag gctggtgtgc tcccacgact cccaccacgc accggagacc agctggtcac
      241 gcaggccctg cgcgccgaac atgcacaaca ccaccacgac ggtgtcggtg acgaacagtc
      301 gccggcggta gcgctgggtc caggtcgtgg tacgggccgg ggccggcccc ggtgctggtg
      361 cggtgcggtg tcggtcgagg gtgcggtgtc ggtcgagggt gtggggcgcc gcagcaccac
      421 tggatcggtg gacgggcccg ggcgtgctca tcaggcctgc ttctgggtgc gacggtagtg
      481 gctcggtggc tccaggccga gagcagcgaa ggaccggcgc agggccacgt cactgggata
      541 gccgctcacc ttggcgacgg tggcgatgtc gagcacacag ccctccggcg atcgcagcac
      601 ggcgatcgct gccgtggctc gtgcactgcg gatctcggcg gccaggctgg actcgacctc
      661 ggcgaaccgg cgctgcagtc ggcgcagcgg cacccccagg ctgcgggcga ccatcaccgg
      721 gctgagccgc ggatcccgtg cgtaggcagt gatgtggtga cgggcccgct cgacgagcga
      781 tcgatcggcg aacggcaccc cgggtgccgc tgcggtctcc agcagcaccg agccgatcat
      841 ctcgtgcagc agcttctccg cgaagtatgc cgacaatgac tgccacggca gcggctggtc
      901 cagcagcgag tcgatgaact gccacatcgg tggcgccaag gtcgaccccg ggtgcacctc
      961 caggctgcgg cgtggggtct gtacggcgaa ctcgctcagc aggctgaccg gcaggatgac
     1021 cccggtgacg gtggcacggc ggctgctggc gctggccacg tggtcggcgg tgttggtgac
     1081 gaggagcaac tgctgcgggg agactgccaa tcggcgtccc cgggccgaca ccaggatcgg
     1141 ctcggatgca cgggccagca gcaggcactg ctgaccggcg ggtgagcgtt gcagccgggt
     1201 gagtgggccc aaccgggtgc ggaacaggtc caggtcgccg atgctgaggc cgaccacgtc
     1261 atcggtggtg ccgggttggt ccggggtcgg gaagcggcgg ctggctcggg tgctcggttg
     1321 tgccgggatg aacggcagca cccggggcac gatcggctcg tgctggcgga tcggctcggc
     1381 gatgctcatg cccgtacccc ctcggccgtg cggatgttgt cgatcaggac ggtcaccgcg
     1441 tggctgccga gcgccggacc gtcgtccagg ctgcccgggc cgtcccggcc gacggcgacc
     1501 gctgcgggat aggtggtgac gccgagccgc tcggtgagcg agccgtcgac gtcgtgatgg
     1561 aggaaggagc ccaacagggg aaaggatccc agtgcttcgg cggccggaag cggagtgacc
     1621 gcccggagct ggacgccgag cccggcgagc tcgctgcgca gtcccggcag ttgctcggcc
     1681 aacgaacggc aggcggcgca gctggtgctg agcagcacca gcacactgcg ctgctcaccg
     1741 gtgaaggtgc gcacgtccgg gctggcgccg aagatgtcgg gaagcttgtg ctcccccagc
     1801 atcggaccct ccgtcgcagg gtcggggacg ggcccgggct ggtgatcgtc ggaccgtccg
     1861 cgccgggtct gtccggtcga ccggccgagg agcaccactg caccggcccc ggccaccagc
     1921 aggcccagct gcagcaggcc caggtcagcg ccccgcaacg agggggtgat ggtggcgatc
     1981 gatcgaccgg cggcggctga cgaaccggtg agggtgacca gaccgaggag cgagaccacc
     2041 gccagggcga ggaacccgcc ggtgcgcacc acggtgcggg ccgtgaccgg ctcccggtcg
     2101 agtgcgccga agcagtggca gtggaccggt tcggcgaacc gggcggccct ggccaccacc
     2161 agcaggtgtc cgatcatcat cgcggtggcc acggctgcgg ccgcggcggc ccagcggccg
     2221 gggagcacca ccagactgac ggcgacggcc acttcgagga tcgcccagga gaccgcaaat
     2281 gcctgtgtgg acagcgatcc gggcagttgc aggtcgtgca gggcccggtg gctgcggcgt
     2341 cggtcggcga gcttggcgac cccactgact gcgaggacca gggcgagggc gcccacgacg
     2401 aggacgagga cggcggcatt catcgcggga cggacctgtc ggcgaggatc gggtggtgcg
     2461 acatcgagac ttccattgcg tgctgctggt ggatgccaga cgtcgcccgg accgtgtcgg
     2521 gggcacgatc cgggcgacgg gcctggcggg aggagggtgt ccggacggcg tggcagcagg
     2581 tgccgcccgg gttcggggga ggaccgaggg tcaggcggtg aggcgctcgc agttggccac
     2641 cgtgccgaag atcggaacgg tcacgccggt gaccttcatc ccggtggcat tgttggccgg
     2701 gtcgttgtcg ttgccgaggt aggccaggta gaaggtgctg gccacgttca gctgaatgcc
     2761 ttggtactcc acggtcagtg ggtccccggg ggcgatgtcg cgcggggtct cgaactcggc
     2821 gacctgggtg ccggtgccca acaggtcgag ggacagtgcc gttccgccac cggtcgtcca
     2881 gttgccgatg cccaggctca gcggggcgct ggagtacagc aggaagcggg tgccggccgg
     2941 gatcgtgccg ccactggcgt cgatctcgaa cgacgcagcc gggccgagcc cgacagtttc
     3001 gcaccagccg tagtcgacga tgacgtcgaa gtcgaggtcg gggacctcct cgccaccacc
     3061 cgcggcgtcg aagcagatga acaccccgag cgagccgagc ccgacaccgc cggtgttcat
     3121 gatgccgatg tcatcggtct gcacgtcgtc gggcgtgatg atggtggtgg tgtaggtgcc
     3181 gatcacgttg agcgtgaagc cggtgagccc gatccggatg ctctgaccga tggtcaggtc
     3241 ctgctgggtc tcgaaggtcg cgaccccgtt gccgatgccg agcaggttca gttggaggcc
     3301 gtcgaagacg aagttgccgg cgctgatggc cacggtgccg ccgctggtga tcgtgatctg
     3361 ggtgccggca ggaatggtgc cctcggtcac ggtgtaggtg aacccgggat tctcacctcc
     3421 gacccggatg gtgaccagcg agcagtcgct gaccgacacc gccaggttgt ttccggtcgg
     3481 acttgcggcc tgcgccggga cggcggaggc gacggcgacg gccggcacgg tccacgccgc
     3541 acccttcacg acggtacggc ggctgacacc cgacgggtcg ggggaaagcc caggattggg
     3601 cgaactcacg gattcctcca agatgtggtg aggctgggcg cttggtcacg gccgagtgat
     3661 cccccgacca gccgcgacat cccggaatca accacatcct gacacacccc caacaggggt
     3721 tggcacaaaa aatgccgtcg tctgacgcgg aacggtgcgg tccgtcatcg aatagttacg
     3781 gacaggtcac gaccgggatt ggtccacgct gctgcgcgcc tgtcgtacgg gcgcgctccg
     3841 ggcagcgctc ggtagcctgc ggggtgtcgt ccacggactg gtccgggcgg caccacccca
     3901 ccagcaagga agacgacgtg cactccttca ccgtacgggt cctcggccgc gcgatcggcg
     3961 tggactgtga tcccaccacc gttccggacg atgtcgccga tgagctgtcg cgccagtggg
     4021 agcagtgcct caccgatgcc gatccggacg accgcgtgtg ggtgagcctg gacgaggccg
     4081 gtccaccgcc ggaccaccgc tacttcgtcc gcggctccga cccgcgggtg gcggcccacc
     4141 gactctccga cgtcgtggtc ggcacggcca tggcggcgtt ggtgccgccc gaggggcggg
     4201 accggccgct ggcccagccc ccgtcggtgc tcggcgtcca cgcggcagcc gtcgtccggg
     4261 accgtcgggc ggtgctgttg gtcggcggca cccaccgcga cgaggcgatg cgcgccctcg
     4321 ccccgcacta cgactacctg tccgactccg cggtcgccgt cgacctggat cggggcaccg
     4381 tgctgggtgc cgccaccccg ctcaccacat ttcccgagcc ggagctggtc tcgcggaccg
     4441 aggtcccgct ccgcgatgcc gggttgcgtc cggtcctcga gccggtgccg ctcgcagcgg
     4501 tcgtggtgct ggaaccgacc gcatccgcgg gcgcggccgg gtggacgccg ctgtcgatcc
     4561 cggtggccac ggcccggctg gcgccggcgg tgccgatcct gcccgccttg ccgcagccga
     4621 tgcaggacct tgcccgggtc gcctcgttga cccggggagc ggtcagtgtc tccggggcgg
     4681 ccccggagct gcccgcactg ctggacgagg tgttggccgt ggagatggcg gccgagctgg
     4741 tgatcccggt cgagccggga ccgggcccgg acaccacgcc gcgcccgggg gatgtgcgac
     4801 ggcaccggat gaccgacggc atcagcgacg gcagcacgct ggcattctgg accaccgagc
     4861 acacggcggt cgcggtcggt gggatcgcac cccacctgtg ggatgccgcg cgccagtgga
     4921 tcggtccgga cgagctcacc gaccgggtcg tgcaggccgt gggcgctccg gagggggccg
     4981 acccgcgcgc tctggtcgac g
//
LOCUS       DATGUK010000068         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1585935,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000068 DATGUK010000000
VERSION     DATGUK010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585935"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="VK103_01045"
     CDS             <1..1768
                     /locus_tag="VK103_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82094.1"
                     /translation="GPSHDPGPSYDPGPSYDPGPSRAPAPDLTHGAGSDSSGSGDGSS
                     SNEPRNPGTSAAGYTPPTQPPPGASSPGGGPLPGGSGGGPSLGSGGGAPPPGAGPGSS
                     GGPAPAPGPPATGGLVGNPAPPPPTAPSAPVGPSLTTSTHPIAPAQPTPPANVTAQAV
                     PPAGQQQPTVPPTAKQVPPGATAAPDRIREPAPPATERAAPARTGMAGAAVPLGATGL
                     APLFGDLRQRTPAPQDLTSALTLLPTQFGPDDGPLAALPPGWEVPYQKVLLPGEADAY
                     LSGQVTTVRGLLHPYDQVRDLTTPAELHAMLGLGFAVGDGRGGTELAFDPASDGIELM
                     RFAGVPSGDLVIPVSEETDPGTGSSIRPLVRHHARPWSGGGLAPGSSEDRIVEEFEVL
                     GELGVPVPHLAEIWRVYADGSSEHLGTHNARTGLWRRTTVSAPPVGVALPHGPQAVMP
                     DGARFSTVMLNDHEHVLVDRTGHAPDFFTDAPDGSHRHVVASDQIAAVVGVATIGTWR
                     GARVLVLQQVAGQRLVDYIGDDPRQAAALGLRQLGQGQWQQTWVPTTEVVGARTWERN
                     YPPLPVTPSRPVAPAPAPVDAG"
     gene            complement(1716..2210)
                     /locus_tag="VK103_01050"
     CDS             complement(1716..2210)
                     /locus_tag="VK103_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HLQ82095.1"
                     /translation="MTFFADWPYPIAVAALFVIVMARANGTYWLGRGLRSGAGRTRMS
                     RILESPGYRRAERLVQRWGAPAVTLCFLTVGVQTMINIAAGAGRMPLRRYLPAVTLGC
                     VIWAFVYATVGFVTFEALLVLHRASPVAFWIVLAAVVVSIGVVVLVNLRRRAQGPEQP
                     DGSA"
     gene            complement(2212..2526)
                     /locus_tag="VK103_01055"
     CDS             complement(2212..2526)
                     /locus_tag="VK103_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HLQ82096.1"
                     /translation="MLGAPLRYLTDRLVQARHTSAWPWGTLTVNVLGSLLLGLLVAAS
                     GPLALLAGTGFCGAYTTWSTAAHESAGLLRRRHLGYGIGYPVASVVLCVLAAGVGAVL
                     LP"
     gene            complement(2761..3093)
                     /locus_tag="VK103_01060"
                     /pseudo
     CDS             complement(2761..3093)
                     /locus_tag="VK103_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
BASE COUNT          461 a         1209 c         1103 g          346 t
ORIGIN      
        1 cggtccctcc cacgaccccg gtccctccta cgatccgggc ccctcctacg atccgggccc
       61 ctcccgcgcc cccgcacccg acctcaccca cggtgccggc agtgacagca gcggcagcgg
      121 ggacggatcg tcgtcgaacg agccgcgcaa ccccggcacc tcggccgccg gctacacccc
      181 gcccacccaa ccgccaccgg gggcgtcgtc ccccggaggt ggtccgttgc ccggtgggtc
      241 cggcggcggc ccgtcgctcg ggtcgggcgg cggcgctccc ccgccgggtg ccgggccggg
      301 cagcagcggt ggtccggccc ctgcaccggg cccaccggcc accggcggac tggtcggcaa
      361 tccggccccg ccacctccca cggcaccgtc ggccccggtc ggcccgtcac tgaccacctc
      421 cacccacccg atcgcgcccg cgcagccgac gccgccggcc aacgtgacgg cccaggccgt
      481 accaccggcg ggccaacaac aaccgacggt gccccccact gccaagcagg tgccacccgg
      541 ggccaccgcc gctccggacc ggatccggga gcccgcccca cctgcgaccg agcgggcggc
      601 ccctgcccgt acggggatgg ccggggccgc ggtgcccctc ggcgcgaccg gcctggcccc
      661 gttgttcggc gacctgcgac agcgcacacc ggcaccgcag gacctcacgt ccgcgttgac
      721 gttgctgccg acccagttcg gtcccgacga cggaccgctc gccgcgttgc cgcccgggtg
      781 ggaggtgccg taccagaagg tgttgttgcc gggcgaggcc gacgcctatc tgtccggtca
      841 ggtgaccacc gtgcgagggc tgctgcaccc gtacgaccag gtgcgtgacc tcaccacccc
      901 ggccgaactg cacgccatgc tcgggctggg cttcgccgtc ggcgacggcc gcggtggcac
      961 cgagttggcg ttcgacccgg ccagcgacgg catcgaactg atgcggttcg ccggcgtgcc
     1021 cagtggtgac ctggtgatcc cggtcagcga ggaaaccgac cccggcaccg gttcgtcgat
     1081 ccggcccctg gtgcgccacc acgcgcgacc gtggagtggt ggcggactgg cgccgggctc
     1141 gtcggaggac cggatcgtcg aggagttcga ggtgctcggt gagctgggcg tgccggtccc
     1201 ccacctcgcc gagatctggc gggtgtacgc cgacggcagc agtgagcacc tcggcaccca
     1261 caacgcccgg accggtctct ggcggcgcac cacggtgtcc gcgccgcccg tcggggtagc
     1321 cctgcctcac ggcccgcagg cggtgatgcc ggacggagcc cgcttcagca cagtgatgct
     1381 gaacgaccac gagcacgtgc tggtggatcg gaccgggcac gccccggact tcttcaccga
     1441 cgccccggac ggcagtcacc ggcacgtggt ggcctcggac cagatcgctg ccgtggtggg
     1501 cgttgccacc atcggcacgt ggcggggcgc ccgggtgctg gtgttgcagc aggtggccgg
     1561 ccaacggctc gtcgactaca tcggcgacga tccgcggcag gcggcggcgc tcggcctgcg
     1621 tcaactcggg cagggccagt ggcaacagac ctgggtgccg accaccgaag tggtgggtgc
     1681 ccgcacctgg gagcgcaact acccgccgct gccggtcacg ccgagccgtc cggttgctcc
     1741 ggcccctgcg cccgtcgacg caggttgacc agcaccacga ccccgatgct gacgacgacc
     1801 gcggcgagca cgatccagaa ggcgaccggt gaggcccggt gcagcaccag cagggcctcg
     1861 aaggtgacga acccgaccgt ggcgtagacg aacgcccaga tgacgcagcc gagcgtcacc
     1921 gcgggaaggt aacgccgcaa cggcatccgg ccggcgcccg ccgcgatgtt gatcatggtc
     1981 tgtacgccga cggtgaggaa gcacagcgtc accgccgggg caccccagcg ttgcaccagc
     2041 cgttcggcgc gccggtagcc cggcgactcg aggatccgtg acatccgggt ccgaccggca
     2101 ccggagcgca atccccgacc cagccagtag gtgccgttgg cccgtgccat cacgatcacg
     2161 aagagggcgg ccaccgcgat cgggtagggc cagtcggcga agaacgtcat ctcagggcaa
     2221 gagtacggcg ccgaccccag cggcgagcac gcacagcacc accgaggcca ccggatagcc
     2281 gatgccgtac ccgaggtggc gtcgacgcag cagtccggcc gactcgtggg cggccgtgct
     2341 ccaggtggtg taggccccgc agaagccggt cccggccagc agcgcgagcg gtccggaggc
     2401 cgccacgagc aggccgagca gcagtgaacc gagcacgttg acggtcagcg tcccccacgg
     2461 ccaggccgac gtgtgccggg cctgcacgag tcggtcggtc aggtagcgca ggggtgcgcc
     2521 cagcatggcg ccgagcgcca cccagccggc ggccccgtcc gcaccggcga gcccggtgcc
     2581 gaccaccacc acggcagcga tcccgatcgt ccccaccgcg gttgtggcca cgcccgcacg
     2641 ggatcgccgg gacgtccccg ggggttggct ggcccgggcc cgcgcccgcg acgtgagcca
     2701 ccagcccact gcggccaacg acaccgcgcc gatcatggtg gccaccgcgt agccgagggc
     2761 cagcccgggc cgccccgact gggcgagcgc gacggtcccg accgcctgcg tggacatcgt
     2821 ggtgaaccct ccgaggacgc cgacgccgag gaacggccgc accagggcgg tcgcggtgcc
     2881 ggcagtcggg gggcgtgcgg cgagcaggcc catcagcacg cccattgcca gtgcgccgag
     2941 caggttgacc agccaggtgg cccacggcca cgcagccggc gagctcggca accacaccgc
     3001 gaccaggtgg cgcagcgagg ccccggccgc cccaccggcg gcgatcaccg ggaggacgac
     3061 cagatgggtg tcgccgagct cccgccgctg caccggaacg tccaggtcga catcagggt
//
LOCUS       DATGUK010000069         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1472409,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000069 DATGUK010000000
VERSION     DATGUK010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472409"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VK103_01065"
     CDS             <1..816
                     /locus_tag="VK103_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLQ82097.1"
                     /translation="VDARLLFDSDVRTPLGQVRLVGRAAGTARNQGRVMNEYALVYSL
                     AGVADYRDELGTVGTLSPETAMFVFPGLRHWYGATGGNSWEQVYVVFGGPVFHLMEAQ
                     GLLERSSPITRLRPIDDWLARFLALADRPRPVTQADAMIMVGDFLRLLLMTVANTPQR
                     TTWLTQARSLIDNTTSGPADFDAIAAELGTSRETFRKRFRKEAGEPPVRYRNRRRIQI
                     AQDLLTATGLSLRQIAATLGYTDEFHLSKRFKAATGLSPREYRVERDASRGRD"
     gene            complement(909..>2044)
                     /locus_tag="VK103_01070"
     CDS             complement(909..>2044)
                     /locus_tag="VK103_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide lyase family 8 super-sandwich
                     domain-containing protein"
                     /protein_id="HLQ82098.1"
                     /translation="TRGYETMSMENIKGWHQGSAARYLYVDDDQGQYIDYFPTLDPFR
                     YAGTTVDSQELTEIRNSAPTYSSFVGGVQIGGHRLDDGFYDAPEYASWVQQLSSRDST
                     MTAKLSWFVIGDVIVHLGADIRGGSGAPVETIVENRALKSDAARLLTINGEPVAESAE
                     PGWQATLDDVASVHLPNAAGIVMLDRSAPVHFLHEEREGRFSDLSPDRGSDTLCRNAF
                     HTIWVDHGPTPDGAAFGYLYLPLATAEQTAALASDPEVGVLENSSRIQAVESRRLGLL
                     AAHFHAAGTVRSGPYQVTGQQESAVMLREYGRGRGRLEIAVSEPTQDVERVEVTVVGP
                     RLASATVVASDPEVSIGRDGRRMRLSVDTSVPDGSSKHVWLDL"
BASE COUNT          320 a          727 c          669 g          328 t
ORIGIN      
        1 gtcgacgcga gactgctgtt cgacagcgat gtgcggaccc cgttgggcca ggtgcgcctg
       61 gtcggccggg ccgccggcac cgcccggaac caggggaggg tcatgaacga gtacgcgctt
      121 gtgtactcgc tggccggtgt cgccgactac cgcgacgagt tgggcacggt cggcacgctc
      181 tctccggaga cagcgatgtt cgtgttcccg gggctgcggc actggtacgg cgcgaccggc
      241 ggcaactcgt gggagcaggt gtacgtcgtc ttcgggggcc cggtcttcca cctgatggag
      301 gcacagggcc tgctcgagcg gtcgtcgccg atcacccggc tgcgaccgat cgacgactgg
      361 ctggcgcgct tcctcgccct ggccgaccgc ccccgaccgg tcacccaggc cgacgcgatg
      421 atcatggtgg gcgacttcct ccggctgttg ctgatgacgg tcgccaacac accacagcgg
      481 acgacgtggc tcacgcaggc gcgcagcctg atcgacaaca ccacctccgg cccggcggac
      541 ttcgacgcca tcgccgcgga gctcggcacc agtcgcgaga cgttccgcaa gcgattccgg
      601 aaggaggccg gcgaaccacc ggtccggtac cgcaaccggc ggcggatcca gatcgcccag
      661 gacctgttga ccgccaccgg gctcagcctg cgccagatcg cggccaccct cggctacacc
      721 gacgagttcc acctgtccaa gcggttcaag gcggccaccg ggctctcacc acgcgagtac
      781 cgcgttgagc gtgacgcgtc ccgaggacgc gactgagccg atccgaggtg gaggtcgtac
      841 acgactcgcc catgtcgcca accgagcacg atgaccccaa cggcagcgcc ggcgcggccg
      901 gacctgactc acaggtccag ccagacatgc ttgctggatc cgtccgggac cgacgtgtcg
      961 acgctcaacc gcatccgacg cccgtcccgc ccaatgctga cctcggggtc ggatgcgacg
     1021 acggtggcac tggccagtcg gggaccgaca accgtcactt cgacccgttc cacatcctgg
     1081 gtcggctcgc tcaccgcgat ctcgagcctg ccgcgccctc gaccgtactc gcgaagcatc
     1141 accgccgact cctgctggcc cgtcacctgg tacggaccgc tgcgcaccgt tcccgccgcg
     1201 tggaagtggg ctgccaacag gccgagtcgt cgagattcga cggcctggat ccgggacgag
     1261 ttctccagca ctcccacctc agggtcgctc gccagggccg cggtctgctc ggccgtggcc
     1321 aacgggaggt acaggtagcc gaatgccgcg ccgtccgggg tgggcccgtg gtccacccag
     1381 atggtgtgga acgcattgcg gcacagtgtg tcactccccc ggtccggtga caggtcgctg
     1441 aaccgccctt cgcgttcctc atggaggaag tgcaccggcg cgctccggtc cagcatcacg
     1501 atccctgcgg cattcgggag gtggaccgat gcaacatcat ccagtgtcgc ctgccagccg
     1561 ggctcggccg actccgcgac gggctcgccg ttgatcgtca gcagtcgcgc ggcatctgac
     1621 ttcagggcac ggttctcgac gatcgtctcc accggtgccc cgctcccgcc acggatgtcg
     1681 gcacccaggt gcacgatgac gtccccgatc acgaaccagc tgagcttcgc ggtcattgtc
     1741 gagtcccgac tgctgagctg ctgcacccag gacgcgtact ccggcgcgtc gtagaagccg
     1801 tcgtcgagcc gatggccgcc gatctgcacg ccgccgacga aggagctgta cgtcggggcg
     1861 gagttcctga tctcggtgag ctcctgggag tcgaccgtgg tgccggcgta acggaacgga
     1921 tcgagggtcg ggaagtagtc gatgtattgg ccctgatcgt catccacgta caggtagcgc
     1981 gccgccgacc cttggtgcca ccccttgatg ttctccatgc tcatcgtctc gtagccgcgg
     2041 gtgc
//
LOCUS       DATGUK010000070         2398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1586493,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000070 DATGUK010000000
VERSION     DATGUK010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586493"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VK103_01075"
     CDS             <1..645
                     /locus_tag="VK103_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLQ82099.1"
                     /translation="LRWLRGRPDAVPERFRTVLAVPAALAVAVTGQALGLAWWLAWFL
                     GVAVATAGWIGLGQVEADEHRRRRLWLVEELPATCDLLAVCLDAGLPLRRACTVVAAA
                     MPGPVAEDLGRVHALVAAGTHEPDAWRSLAEAEPWRRLVIDLARSVESGSAVAAVLHH
                     HAEDARVAAELAREVRARTAGVRSVLPLMLCFLPAFFLVGVVPLVASLVLRMLA"
     gene            804..1019
                     /locus_tag="VK103_01080"
     CDS             804..1019
                     /locus_tag="VK103_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013170879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HLQ82100.1"
                     /translation="MSDNCTTQHQTHGTDAERDERGMTTAEYAVGTVGAVTAAGTLIW
                     IFQQDWFRELLMAIIKAIILLVTGLDV"
     gene            1114..1491
                     /locus_tag="VK103_01085"
     CDS             1114..1491
                     /locus_tag="VK103_01085"
                     /inference="COORDINATES: protein motif:HMM:NF041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family type IV pilus minor pilin"
                     /protein_id="HLQ82101.1"
                     /translation="MINDGSGSRPGRGMVTAELAVGLLAVALVISMAAGLAGTLQLQA
                     RCIDSAAEIARQIARGDDAAAARASRDVPEGAVVSHHRSGGQVVVTVRMPVRLANLPL
                     FEVSATARTIVEPGEAGGTEQGP"
     gene            1488..1892
                     /locus_tag="VK103_01090"
     CDS             1488..1892
                     /locus_tag="VK103_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HLQ82102.1"
                     /translation="MTDRRRPFVADERGSGSMLTLMTVALTLMAFLVVLVVGSYLIAD
                     HRAVAAADRAALSGARAHGAPGGRQQEASSACETARRVAELNGARMTGCRITGDRADY
                     VVQVTVVVDVGVLWPGLPAQVPGSAHAGRLSG"
     gene            complement(1900..2283)
                     /locus_tag="VK103_01095"
     CDS             complement(1900..2283)
                     /locus_tag="VK103_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82103.1"
                     /translation="MLPVAIVLLVLILVFVVGAVVSNPEPVTLDVFVAQIPTNLAVVF
                     IVGMVSALALAAALGLMWFGLRRQRSRAQAARSSRTPRPATSGRGADKEVATPKKGGT
                     SGGADSRQGTAEPTRSGKDRADRDR"
BASE COUNT          311 a          814 c          927 g          346 t
ORIGIN      
        1 ctgcggtggc tccggggccg cccggacgcg gtgccggagc gattccgcac cgtgctcgcg
       61 gtcccggccg cactggccgt cgccgtgacc ggccaggcac tcgggctggc gtggtggctg
      121 gcgtggtttc tcggggtggc cgtggcgacc gccggatgga tcgggctggg gcaggtcgag
      181 gccgatgagc accggcgccg acgcctgtgg ctggtcgagg aactgccggc gacctgtgac
      241 ctgttggcgg tgtgcctgga cgccgggttg cctctgcgcc gtgcctgcac ggtggtggcg
      301 gcggcgatgc ccggcccggt cgccgaggat ctcgggcggg tgcacgccct ggtcgcggcc
      361 ggcacgcacg aacccgacgc gtggcgcagc ctcgccgagg cggagccgtg gcgtcgcctg
      421 gtgatcgacc tggcgcgctc ggtcgagtcc gggtccgcgg tggccgcggt gctgcaccat
      481 cacgccgagg acgcccgtgt cgccgcagag ctggcccgtg aggtgcgggc caggacggca
      541 ggggtgcgca gcgtgttgcc gttgatgctg tgcttcctgc ccgccttctt cctggtgggc
      601 gtggtgccgc tggtcgccag cctggtgctg cggatgctcg cctgaccggg gcgacgacga
      661 gttgtccaca gtacgacgtg ccgaaccgag cctcatgcac agctcacagg tccgggtgag
      721 ccatccgctc agttccccac cactgttcgg atgtagggcc cggcacccgc cgacgcccga
      781 catctcgatg gagggagcac ccgatgtccg acaactgcac cacacagcac cagacccacg
      841 gcaccgacgc cgagcgggac gagcgcggca tgaccaccgc cgagtacgcg gtgggcaccg
      901 tgggtgcggt gaccgcagcc ggaacgttga tctggatctt ccagcaggac tggttccggg
      961 agctgctgat ggcgatcatc aaggcgatca tcctgctcgt caccgggctc gacgtctgat
     1021 ccgcggggcg cggagccggg cacgccaggg gtggggacgg agccgtcccc gcccctgcgt
     1081 ggatcccctg caccatgagg gagggagcac atcatgatca acgacggatc agggagccgc
     1141 ccgggccgtg gcatggtgac cgcggagctc gccgtcggcc tgctcgccgt cgccctggtc
     1201 atctcgatgg ccgccggcct cgccggtacc ctgcagttgc aggcccgctg catcgactcg
     1261 gccgctgaga tcgcccgcca gatcgcccgt ggggacgatg cggcggccgc ccgggccagc
     1321 cgcgacgtgc cggagggcgc cgtggtcagc caccaccgat ccgggggcca ggtggtggtg
     1381 acggtacgga tgccagtgcg gctggcgaac ctgccgctgt tcgaggtctc ggcgaccgca
     1441 cggaccatcg tcgaaccggg ggaggccggc gggacggagc aggggccatg accgatcggc
     1501 gccggccgtt cgtcgcggac gagcgcggat ccgggtcgat gctgacactg atgaccgtcg
     1561 ctctcacgct gatggcgttc ctcgtcgtgc tggtcgtcgg cagctacctg atcgccgacc
     1621 accgggcggt ggcggccgct gaccgggccg cgctgtcagg cgcccgtgcc cacggcgcac
     1681 cgggcgggcg gcagcaggag gcgtcgtcgg cgtgcgagac cgcgcgccgg gtggctgagc
     1741 tcaacggagc ccggatgacg ggctgccgga tcaccggcga ccgggccgac tacgtcgtgc
     1801 aggtcacagt cgtggtcgat gtcggcgtgc tgtggccggg cctgccggca caggtgccgg
     1861 gcagtgccca cgccggccgg ttgtcgggct gagggacggt cagcggtccc ggtcggcacg
     1921 gtccttgccg gaccgggtcg gctcggcggt gccctgtcgg ctgtcggcgc cgccggacgt
     1981 cccgcccttc ttcggggtcg cgacctcctt gtcggcacca cggccgctgg tggccgggcg
     2041 gggcgtccgg ctgctgcgtg ccgcctgcgc gcgggaccgc tgacgtcgga ggccgaacca
     2101 catcaggccg agcgcggcgg cgagggcgag ggcggagacc atcccgacga tgaacaccac
     2161 ggccaggttg gtcgggatct gggcgacgaa cacatcgagc gtcaccggtt cggggttact
     2221 gacgaccgca ccgacgacga acaccaggat cagcacgagc aggacgatgg ccacggggag
     2281 catggctcat tatccgcgca gcacggttgg gtggcgcaag cctggcggcg gatcgggtcc
     2341 gatgggcgtc tcactgcgga ttcatcgcct gcagcagacg gcgggtcacc ggcagcag
//
LOCUS       DATGUK010000071         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_426037,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000071 DATGUK010000000
VERSION     DATGUK010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426037"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..1731
                     /locus_tag="VK103_01100"
     CDS             367..1731
                     /locus_tag="VK103_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLQ82104.1"
                     /translation="MPEQLTTASDAATDPGPAYVLESVTKRYGRQRRHSTVPAALTDV
                     TLTIPRGRTTVVAGPSGSGRTTLLRLLNRTIEPTEGTITVDELPLRRHRRGALRRRIG
                     AVLQGSGLLPRRTVGENLTAAARDAGVPGREVEDRVDHWLAQVGLPPAITDVLPGRLT
                     SGDLQRVKLTRALVADPEVLLLDEPFALLDTGVRGELQTLLLDLQQQRPRTIVLATQD
                     LDEAIRLADRIAVLGRTGRLAQHATPAEVVNAPADDEVAAFVAQDRGSRALAFHPSDQ
                     LPVDRVRAVRTPSAVRHGGEALVLDARARPRGWVTPERPGQVLGLGGTFDPRRDSLRT
                     ALDAALGSPVGRAVTVEADTGRFTGAVRLEAITAHIAELRRTVAIGVLDAAEAERRRR
                     AEEARREAAERARRQQAEQARLLAEIEGSSPPPRPGSVSVPEPPTDDDAPAPSTPFAG
                     QPGA"
     gene            1768..>2181
                     /locus_tag="VK103_01105"
     CDS             1768..>2181
                     /locus_tag="VK103_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLQ82105.1"
                     /translation="MADAAPRSGLLDSVPLHVEDTGGTGRPVVLLHGWPMSGQVWDEQ
                     VKALQAAGHRVVTYDRRGFGRSGTDFDRYDYDVLADDVAAVFTELDLHDVTLLGFSMG
                     GGEVARYVRRHGQDRLHSVVFAAAVPPYLLQGEDNP"
BASE COUNT          295 a          797 c          818 g          271 t
ORIGIN      
        1 ccgacgacca tcgcgccgac gacgaccagc acgagggaca gcccggcgag atcgtacggc
       61 acgccagcga ccctaacccg gccggctccg accggtccgg gccatccgcc ggtcgaaccc
      121 gcggacgcca gcaccccctc gcagtgccac ggatcggccg gccgggccgg gtggtggctg
      181 ggcgggggtg gatttcggcg attcggtgca cgggcacgcc gtcgcggagc cgacggatcc
      241 cggtggtcag cggttatggt catggtgacc ggaacttccc cgccggtggc cgtggcggac
      301 cgggcgccgg gcacgaccgg gcacccgggc cacagggctg ccggcccgtc gcgaggtgtg
      361 actcccgtgc ccgagcagct gaccaccgca tccgatgccg ccaccgatcc cgggccggcc
      421 tacgtgctgg agtcggtgac caagcggtac gggcgtcagc gacgccacag caccgtcccc
      481 gcggcgctca ccgacgtcac cctgacgatc ccgcggggcc gcaccacggt ggtggccggc
      541 ccgtccggca gtggtcgcac cacgctgctg cggctgttga atcgcaccat cgagcccacc
      601 gagggcacca tcaccgtcga cgagctgccg ctgcgtcgcc accgtcgcgg agcgctgcgc
      661 cgccgcatcg gggcggtgct gcagggcagc ggtctgctgc cccgccgtac cgtcggcgag
      721 aacctcactg cagcggcccg ggacgcgggc gtgccggggc gggaggtgga ggaccgcgtg
      781 gatcactggt tggcgcaggt cgggctgcca ccagcgatca ccgacgtgct gccgggccgg
      841 ctcacctcgg gggacctgca gcgggtcaag ctcacccggg ccctggtggc cgatccggag
      901 gtgctgctgc tcgacgagcc gttcgcgctg ctcgacaccg gggtacgggg agagctccag
      961 accctgctgc tcgacctgca gcagcagcgg ccccggacga tcgtgctggc cacccaggac
     1021 ctcgacgagg cgatccggtt ggccgaccgg atcgccgtgc tggggcgcac cggccgactt
     1081 gcccagcacg ccacgccggc cgaggtcgtc aacgcgcccg ccgacgacga ggtcgccgcc
     1141 ttcgtcgccc aggaccgtgg ctcgcgcgcc ctggcgttcc atccgtccga ccagctgccg
     1201 gtggaccggg tccgtgcggt tcgtaccccg agcgcggtcc gccatggcgg agaggcgctg
     1261 gtgctggacg cccgggcccg cccccgtggc tgggtgacac cggagcggcc cggtcaggtg
     1321 ctcgggctgg gcggcacctt cgatccccgg cgggactccc tgcgcaccgc actcgacgcc
     1381 gcgttggggt caccggtcgg ccgggcggtg accgtggagg ccgacaccgg ccggttcacc
     1441 ggcgcggtcc ggttggaggc catcaccgcc cacatcgccg agctgcgtcg caccgtggcg
     1501 atcggggtgc tcgacgccgc cgaggcggag cgccgccgcc gtgccgagga ggcccggcgg
     1561 gaggccgccg agcgggcgag gcgacaacag gccgagcagg ccagactgct cgccgagatc
     1621 gagggcagct ccccaccacc gcggccgggg tcggtaagtg tcccggagcc acccaccgat
     1681 gacgacgcac cggcaccgag cacgcctttc gcaggacagc cgggcgcctg agtacagtcg
     1741 ggagaacagg tcgacgcagg aggactcatg gctgacgcgg cgccccggtc ggggctgttg
     1801 gactcggtgc cactgcacgt ggaggacacc ggcggtacgg gacggcccgt cgtgctgttg
     1861 cacggctggc cgatgtccgg gcaggtctgg gacgaacagg tgaaggcgct gcaggcggcc
     1921 gggcaccggg tggtcaccta tgaccgccgc gggttcgggc gcagcggcac cgacttcgac
     1981 cggtacgact acgacgtcct cgctgacgac gtcgcggccg tgttcaccga gctcgacctg
     2041 cacgacgtca ccctgctcgg tttctcgatg ggcggcggtg aggtggcccg ctacgtacgg
     2101 cggcacggtc aggaccgcct gcacagcgtg gtgttcgcgg ccgcggtgcc gccgtacctg
     2161 ctgcagggcg aggacaaccc g
//
LOCUS       DATGUK010000072         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_783050,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000072 DATGUK010000000
VERSION     DATGUK010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783050"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..461
                     /locus_tag="VK103_01110"
     CDS             147..461
                     /locus_tag="VK103_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HLQ82106.1"
                     /translation="MAGQSSVDRAEMQKAAGQIDEALGTISGIQRTLGSNVQGLMATW
                     KGNAATAFLKAFTEFDQQFSVVLNELEGIHGKLTDTQSKYTQTEADQEAATAGIRAAL
                     NG"
     gene            500..790
                     /locus_tag="VK103_01115"
     CDS             500..790
                     /locus_tag="VK103_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HLQ82107.1"
                     /translation="MSDFTQVNAGAMQQGIGDLQTAYNGTESTLTDLEQQLEASLAQW
                     DGGAREAYGVAKANWDRAANHMAQVIQKMQTTLTSISDNYDSNERNIAGSWQ"
     gene            993..1376
                     /locus_tag="VK103_01120"
     CDS             993..1376
                     /locus_tag="VK103_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82108.1"
                     /translation="MYQHDGGGGGSTAPKVTSDVTVDPGEIRAFAKFLGEMETELKSI
                     RTTVQDGKSVPAKAFGVYHASESAAQRHTKVLDEELTFLDKVVKRMGEVSDGTTQLSK
                     IYTDLAELNQANSDAVSAHLKSTAQ"
     gene            1410..2987
                     /locus_tag="VK103_01125"
     CDS             1410..2987
                     /locus_tag="VK103_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82109.1"
                     /translation="MYTHDGGGGGSYYTLRTHRELNAYVASASTIDLDRLRRNWDDSG
                     RNAHQMADLIEAKLAKLTAAGGWTGPGAEAFANTIRKELVANLRDYARRAGGYTPEGE
                     QNVSHAHDLEVISEAVSGSYSAANSHNIPWDVDTTWNVRQKHVDQSLFSHIEEAVTGE
                     DEDYEKAKKNAPYEIVNGGNSVVKEVPKPQYEPIESSVPPNPSSTVYEATNTSVAPTV
                     KRFDLILERLGLGEGPKQHVYTAVNNVHSVLSSYAPEQVTEADTTDKGTSTGAGATNG
                     PGAHGGGGGAGTSPGRNFGPPGGGGGGTGGGGGDHGGGTGGGYDGPDHDTNPGGPIGG
                     PGTGGGDGGHDINPGGPIGGPGDGGGVEPPLTGGVGGGETGAAGAGPSAPPVGGIGAP
                     APAPAAAPVSVGAGPGVGGLGSLGPGAMNAGAPGTSFGIGADGRPTVNTGKVGAIAGG
                     ANTPDGAGVRGAGSGAGGAHGAGPHGAGMPASGARGRRTGSSGDDEQEQELDETWLEE
                     DESVWGNRAAAPPSEIR"
     gene            3080..>3308
                     /locus_tag="VK103_01130"
     CDS             3080..>3308
                     /locus_tag="VK103_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HLQ82110.1"
                     /translation="MVPVPEMPEVESLRRFLAERCVGHAVAQVDLAAISALKTYDPPF
                     TALQGLVVQGVQRHGKFLALDCGGLFLAFHLA"
BASE COUNT          609 a         1180 c         1125 g          394 t
ORIGIN      
        1 gttcggggga acggacaaca ccaatctcgc gcaccggcgc ctccctccgg ttcggaagtg
       61 accccctgcc gatctactgt cgacgacagg aactcaccgc tccaccgacc gccggcggag
      121 tgaccacaca caccggagga caacccatgg caggacagtc aagcgttgat cgcgctgaaa
      181 tgcagaaggc cgccggccag atcgacgagg ccctcggcac catcagcggc atccagcgca
      241 ccctcggctc caacgtccag ggcctgatgg ccacctggaa gggcaacgcg gccaccgcgt
      301 tcctgaaggc gttcaccgag ttcgaccagc agttcagcgt cgtcctcaac gagctcgagg
      361 gcatccacgg caagctgacc gacacccagt cgaagtacac ccagaccgaa gccgaccagg
      421 aggccgcgac cgccggcatc cgcgccgcgc tcaacggctg accccgaccg caccgaccac
      481 accaaggagc actgaacaga tgagcgactt cacccaagtc aatgcgggcg cgatgcagca
      541 gggcatcggg gatctgcaga ccgcctacaa cggcaccgag agcaccctca ccgatctcga
      601 gcagcagctc gaggcctcgc tggcccagtg ggacggtggc gcgcgtgagg cgtacggggt
      661 ggccaaggcc aactgggacc gggcggccaa ccacatggcc caggtcatcc agaagatgca
      721 gaccaccctg acctcgatca gcgacaacta cgactcgaac gagcggaaca tcgccggcag
      781 ctggcagtga ggttccctga cgagtgaaca tcgtcaccac gcccagtggc tcgttcccgc
      841 acgccgggga cgagccactg gcgttttcct gaccaagtcc cgaccgagcg gaacggaact
      901 gagcccttca cgcgtcatac tcggaggtgg tgcgccgtca cctcggcgcc cccggacgca
      961 ggaacagcac gaaacaccca agggagacaa ccgtgtacca acatgacgga gggggcggtg
     1021 gcagcaccgc accgaaggtc acctcggacg tcaccgtcga tcccggtgag atccgcgcct
     1081 ttgcgaagtt cctcggcgag atggagaccg agctcaagtc gatccgcacc acggtccagg
     1141 acggcaagtc ggtcccggcc aaggcgttcg gggtctacca cgcctcggag agcgcggccc
     1201 agcgtcacac caaggtcctc gacgaggagc tgacgttcct cgacaaggtg gtgaagcgga
     1261 tgggcgaggt ctccgacggc acgacccagt tgtccaagat ctacaccgac ctggccgagc
     1321 tcaaccaggc caactcggac gccgtctcgg cccatctcaa gtccaccgcg cagtgacgcc
     1381 gaccccagca gacgaggagt gaacgacgaa tgtacacaca tgacggtggc ggcggcggtt
     1441 cctactacac cctgcggacc caccgtgagc tgaacgccta cgtggcctcc gcctccacga
     1501 tcgatctcga ccggctgcgc cgcaactggg acgactcggg ccgcaatgcc caccagatgg
     1561 ccgacctgat cgaggccaag ctggccaagc tgactgcggc cgggggctgg accggccccg
     1621 gcgccgaggc gttcgccaac acgatccgca aggaactggt ggccaatctg cgcgactacg
     1681 cgcgccgcgc gggcggttac accccggagg gcgagcagaa cgtcagccac gcccatgacc
     1741 tcgaggtgat cagcgaggcc gtgagcgggt cgtactccgc ggccaacagc cacaacatcc
     1801 cgtgggacgt cgacaccacc tggaacgtgc gtcagaagca tgtcgaccag agcctcttca
     1861 gccacatcga ggaggccgtg accggcgagg acgaggacta cgagaaggcg aagaagaacg
     1921 ccccgtacga gatcgtcaac ggcggcaact cggtggtcaa ggaggttccg aagccgcagt
     1981 acgagccgat cgagagcagc gtgccgccga acccgtcatc gacggtgtac gaggcgacga
     2041 acacctctgt cgccccgacg gtcaagcggt tcgacctgat cctggagcgt ctgggcctgg
     2101 gcgaggggcc gaagcagcac gtctacacgg cggtgaacaa cgtgcactcg gtgctgtcga
     2161 gctacgcccc cgagcaggtc accgaggccg acaccaccga caagggcacc tcgaccggag
     2221 cgggcgcgac caacggcccg ggcgcccatg gcggtggcgg cggcgccggt acctcacccg
     2281 gccgcaactt cggccctccc ggtgggggcg gcggcggcac cggcggtggc ggcggtgacc
     2341 acggcggcgg caccggcggt gggtacgacg gccccgacca cgacaccaac cccggcggac
     2401 cgatcggcgg ccccggcacc ggtggcggcg acggcggcca cgacatcaac ccgggtggac
     2461 cgatcggcgg ccccggcgac ggtggtggcg tggagccgcc gctgaccggc ggtgtcggtg
     2521 gtggcgagac cggcgcagca ggtgccggcc cgtcggcccc cccggtcggt ggcatcggtg
     2581 caccggcccc ggcaccggcc gcggcaccgg tcagcgtcgg tgccggaccg ggtgtcggcg
     2641 ggctcggcag cctgggcccc ggagcgatga acgccggcgc cccgggtacc agcttcggca
     2701 tcggcgcgga cggccgcccg acggtcaaca ccggaaaggt cggcgccatc gccggtggcg
     2761 ccaacacccc ggacggcgcg ggtgtccgcg gggccggttc cggcgcgggg ggcgcacacg
     2821 gcgcgggccc ccatggagcg gggatgcccg cctccggggc ccgtgggcgc cggaccgggt
     2881 cgtccggtga cgacgagcag gagcaggagc tcgacgagac ctggctggag gaggacgagt
     2941 ccgtctgggg caaccgcgcg gccgcccctc cgtcggagat tcgctgagcc caccggctga
     3001 ccccggcagc ggccggcccc gccggaccac acacaacggc gcgtcgcgag cagtcgcggc
     3061 gcgccgttgt cgtgccatca tggtgcctgt gccggagatg cccgaggtcg agtcgctgcg
     3121 ccggttcctc gccgagcgtt gtgtcggtca cgccgtcgca caggtcgacc tcgccgccat
     3181 ctcggcactg aagacgtacg acccaccgtt caccgccctg cagggcttgg tggtgcaggg
     3241 cgtccagcgg cacggcaagt tcctggctct cgactgtggc gggctgttcc tggccttcca
     3301 cctcgccc
//
LOCUS       DATGUK010000073         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1132290,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000073 DATGUK010000000
VERSION     DATGUK010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132290"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="VK103_01135"
     CDS             complement(<1..1638)
                     /locus_tag="VK103_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82111.1"
                     /translation="MTSQPPPALQAEGPPVRVRPGTFLAAVWRRVVVEPVRHGRLRDV
                     DWPPGLRTVVITGYGALAVASVLALLAGPLRQVLPLAPVTDGVTIPRSAVGLLLLLTA
                     VVLSSFLLACCHGPPWLLLVGMLVGLPAMLVMTVFVVLATTGVPYAAIPALAAIVAIA
                     VLLLVWRRRGFGPWQFPALLALVLTVMAVVAADAALRGFPLGFDVLPGQLGVMITLLA
                     PLATPLTFAAGTAPAEVTVTLASRASSLLGRRYRRSVTYTAMLVAALAAIGQLTRQVL
                     VGRYDQLAAPTVVTTLLVLAGCAGGTAAVVRLARGSWRTDDVADTGGALGWPAGAVLV
                     GAVVPLMGVLVVLMAVSTVVPSLGWSFTALQRSWPVPLTRLLACAVAVGVAVWSARRG
                     RPVRGAVLLCAALALLPMALTGLLDTDRALTVDLDLAGLVVTLLVLGWMTWLAARRRL
                     TETRAVGLLAISIVLVVVPHRQAFGEPLEFLFGPGVVVILFGLWWQLFTASEVGNRHS
                     RQFPRTARVLVLLTSVLLTVLVLAHHSLTRQPAAGGLQ"
     gene            complement(1635..3452)
                     /locus_tag="VK103_01140"
     CDS             complement(1635..3452)
                     /locus_tag="VK103_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HLQ82112.1"
                     /translation="MLGGPYGPHPPPAPPVLRRPSRAATVLTVVVLVLGGVVLGVADL
                     PARSSAGTVPATRWLPPDGASLPGRTGGGDATATSALFAAPEATLSLPATAGATLIGA
                     LGSDLSVTVWRRTVASASGDVHEVYTAGPDVRLLTRTAPRIVGYVGGILLLPHDVRPG
                     SSWESSGSTVHPDGRPWRASFRAGPTDRPECLTTDGEVTVGGDGEGAGRYRVAQTWCR
                     DRGLVRDSWTGPDGSVHTWTAAAQGTPITPDPTVVTADRTGTPASSGDPAGGAVTRLP
                     AVAALPDGGTEPLTGSPAGPPLVTASGLVVVRSGADVVVLTPTGTDVLVVRHRLHPGG
                     TVTAVGVFGDVVVSATTARELVGHHAGTGAVLWRSAIDDVADPVLMRLTDDAALVALA
                     SGQVLAVDLRTGTERWRATALVGQDFPASVAAVAGPQPGRVVLVAASTGSVELRDATT
                     GRVRWTWRTDAVVEGVVLGADRAWVRTDRGLYGIDPATGRATVLRRAPATLPPLVLDG
                     RLVLARPEGVRALDAAGQQVWRQPYGCLSTTVGPGGLLCWQGERVLLLDAGTGAVLAE
                     HAVPEHGAGQLDAAVSGSEVWWRVTRVGPEGWVVFRWSR"
     gene            3525..>3761
                     /locus_tag="VK103_01145"
     CDS             3525..>3761
                     /locus_tag="VK103_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002519059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLQ82113.1"
                     /translation="MESCMSAPVPGRTDPSIIRNFCIIAHIDHGKSTLADRMLQLTGV
                     VEERSMRAQYLDRMEIERERGITIKSQAVRMPWTV"
BASE COUNT          598 a         1474 c         1314 g          375 t
ORIGIN      
        1 ctgcagcccg ccggccgccg gctgccgggt gaggctgtgg tgggcgagca ccagcacggt
       61 caacagcacc gaggtcaaca gcaccaacac ccgggcggta cggggaaact ggcgcgagtg
      121 gcggttgccc acctcactgg cggtgaacag ctgccaccac aggccgaaca ggatgaccac
      181 caccccggga ccgaacagga actccagcgg ttcgccgaac gcctgccggt gcgggacgac
      241 caccagcacg atcgagatcg ccagcagtcc gaccgcccgg gtctcggtga gccgtcgccg
      301 tgccgccagc caggtcatcc agcccagcac cagcaacgtc accaccaggc cggccaggtc
      361 caggtcgacg gtgagggcgc ggtcggtgtc gagcagcccg gtgagcgcca tcgggagcaa
      421 cgccagtgcg gcgcacagca gcaccgcccc acggaccggg cggccacgcc gggccgacca
      481 caccgccacc ccgaccgcga ccgcgcacgc caacagccgg gtgagcggga ccggccagct
      541 gcgctgcagt gcggtgaagg accagccgag cgagggcacc accgtgctca ccgccatcag
      601 cacgaccaac acccccatca gcggcaccac cgcgccgacc agcacggcac cggcgggcca
      661 gccgagggcg ccaccggtgt cagcgacgtc gtcggtccgc cagctgcccc gggccagccg
      721 cacgacggcg gcggtgccac cggcgcagcc ggccagcacc aacagcgtgg tgaccacggt
      781 cggggcggcg agctggtcgt agcggccgac gagcacctgc cgagtcaact gcccgatcgc
      841 ggcgagcgcc gccaccagca tcgccgtgta ggtgaccgat cggcggtagc gacgtcccag
      901 cagcgacgag gcccgcgacg ccagcgtcac cgtcacctcg gccggggcgg taccggcggc
      961 gaaggtgagg ggtgtggcca acggcgccag cagcgtgatc atcaccccga gctgcccggg
     1021 cagcacgtcg aaacccaacg ggaagccgcg cagcgcggca tccgcggcga cgacggccat
     1081 caccgtcagc accagcgcca gcagcgcggg gaactgccag ggcccgaacc cgcggcgccg
     1141 ccacaccagc agcagcaccg cgatcgccac gatcgccgcc agtgcaggga tcgcggcgta
     1201 cggcacaccg gtggtggcca gcaccacgaa caccgtcatc accagcatcg ccggcagccc
     1261 caccagcatc ccgaccagca acagccacgg cgggccgtgg cagcaggcga gcaggaagct
     1321 gctgagcacc accgcggtga gcaacagcag caggcccacc gccgaccgcg ggatggtgac
     1381 cccgtcggtc accggcgcca gcggcagcac ctgccgcaac ggtccggcca gcagcgcgag
     1441 cacactggcc acggcgagcg caccgtatcc ggtgatcacc acggtccgca gaccgggcgg
     1501 ccagtcgacg tcccgcagcc gaccgtggcg gaccggctcg accaccaccc gacgccacac
     1561 ggcggccaga aaggtgccgg gccggacccg gaccggcggt ccctcggcct gcaacgccgg
     1621 tggcggctgc gacgtcatcg tgaccaccgg aacaccaccc agccctccgg tccgacccgg
     1681 gtcacccgcc accacacttc ggatccggac actgccgcgt ccagctgacc agccccgtgc
     1741 tccgggaccg cgtgctcggc cagcaccgct ccggtgcccg cgtccagcag cagcacccgc
     1801 tcgccctgcc agcacagcag cccgcccggc ccgaccgtgg tcgacaggca cccgtacggc
     1861 tgccgccaga cctgctgccc agcggcatcc agcgcccgta cgccttcggg tcgggccagc
     1921 accagccggc cgtccagcac cagcggtggc agcgtggcgg gagcccggcg cagcaccgtc
     1981 gcccgcccgg tggccgggtc gatcccgtac aggccccggt cggtgcgtac ccaggcgcgg
     2041 tcagcaccga gcaccacccc ctcgaccacc gcgtcggtgc gccatgtcca gcgcacccga
     2101 ccggtggtgg cgtcccgcag ctccacgctc ccggtggagg cggccaccag caccacccgt
     2161 cccggctgcg gaccggccac cgcagcgacg gaggcgggga agtcctggcc gaccagggcc
     2221 gtggcccgcc accgctcggt gccggtgcgc aggtcgaccg cgagcacctg gccggaggcc
     2281 agtgccacca acgccgcatc atcggtcagg cgcatcagca ccggatcggc cacgtcgtcg
     2341 atcgcggagc gccacagcac cgcgccggtg ccggcgtggt gcccgaccag ttcacgggcg
     2401 gtggtcgccg acaccaccac gtcaccgaac accccgaccg cggtcacggt gcccccgggg
     2461 tgcagccggt gccgcaccac caggacgtcc gtgcccgtcg gggtcagcac caccacgtcc
     2521 gccccggagc gcaccaccac cagacccgag gcggtcacca acggcggccc ggccggggat
     2581 cccgtcagcg gttcggtccc gccgtcgggg agtgcggcca ccgccggcaa ccgggtcacg
     2641 gcccctccgg ccgggtcgcc gctgctggcc ggggtaccgg tgcggtcggc ggtcaccacc
     2701 gtggggtccg gggtgatggg ggtgccctgc gcggcggcgg tccaggtgtg gacggacccg
     2761 tccgggccgg tccagctgtc gcggaccagt ccacgatccc ggcaccacgt ctgggcgacc
     2821 cggtaccggc ccgcgccctc gccgtccccg ccgacggtga cctcgccgtc ggtcgtcagg
     2881 cattcgggcc ggtccgtcgg tcccgcgcgg aagctggccc gccagggccg tccgtcagga
     2941 tgcaccgtgc tgccgctgga ctcccaggac gagcccgggc ggacgtcgtg ggggagcagc
     3001 aggatgccgc cgacgtaccc gacgatccgg ggtgccgtcc gggtcagcaa ccggacgtcc
     3061 ggacccgcgg tgtacacctc gtggacgtca cccgaggcgc tggcgaccgt gcgacgccac
     3121 accgtcaccg agaggtcgga ccccagcgcg ccgatcagcg tcgcgccggc ggtggccggc
     3181 aggctcaggg tcgcctccgg ggcggcgaac agcgcggagg tggcggtggc gtccccgccg
     3241 ccggtccggc cggggaggct cgccccgtcc ggcggcagcc atcgcgttgc cgggacggtg
     3301 cccgccgacg aacgggccgg gaggtcggcc accccgagca ccacgccgcc gagcaccagc
     3361 accacgaccg tgagcacggt ggccgcccgc gacggtcgtc gcagtaccgg aggagccggt
     3421 ggtgggtgcg ggccgtacgg tcccccgagc atggtcacga cgccatgatg gcccgccgct
     3481 ggttcgccgt gggaggatgt tcactgctgc agtcagtcct cgtgatggaa agttgtatga
     3541 gcgcacccgt acccggccgg accgatccct cgatcatccg gaacttctgc atcatcgccc
     3601 acatcgacca cggcaagtcg acgttggcgg accggatgct ccagctgacc ggggtggtgg
     3661 aggagcgctc catgcgggcg cagtacctgg accgcatgga gatcgagcgg gaacggggca
     3721 tcaccatcaa gtcccaggcc gttcggatgc cgtggacggt c
//
LOCUS       DATGUK010000074         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1591884,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000074 DATGUK010000000
VERSION     DATGUK010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591884"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VK103_01150"
     CDS             <1..304
                     /locus_tag="VK103_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82114.1"
                     /translation="FGIAAHGPDADVDCEIQLQGAPHSHFRFLARAAEQLIIDGAPPY
                     PGAERTLLTGGILDHAMRQARGAVDPATPHLDVAYTVPAHIRDTGAYLPTYQHRDN"
     gene            complement(352..1149)
                     /locus_tag="VK103_01155"
     CDS             complement(352..1149)
                     /locus_tag="VK103_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLQ82115.1"
                     /translation="MIDAHLHLFERGFDGTSEPGAELQAYLRLRQQFGIGPGLVVGYE
                     GQQRFAGNNDHVCALARRHDWIVPLVHLDRERLGADPGLARDWLTAGAAGWSAYVLGT
                     EEARAWDALSDEVWEGLDAPVPWSLNIDPAALAVLTPRLARLQHTTVLLSHLGLPGRA
                     SGTPAERLAPVVAAVRAIGHVRVKVSGLYAVAGRDETPPFPSARPYLQHMRDRVGVEH
                     LVWGSDYTPVLARQSFASQVDLAAYQVFSATELGRLAADDLARMIRR"
     gene            complement(1146..1871)
                     /locus_tag="VK103_01160"
     CDS             complement(1146..1871)
                     /locus_tag="VK103_01160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLQ82116.1"
                     /translation="MEELGLAGRRVLVTGAGSGIGLATSGFLARHGAAVAALDRDTTR
                     CAELGVAHAATCDVTDADGVEAAVAGVAEALGGLDVLIHAAGITREARTDVRDLSEES
                     FRAVLDVNVVGSFLVARAVARVMVPAGAGLIVLIGSGAGVLGSSSSIPYGASKGAVNG
                     LALTLTEHLKGHGIRVMNVMPAQVDTPLVHRSLATGEEHGADPAVAERFKASMVPAAD
                     VGRAIGMLASPLLSHLRGPVLTR"
     gene            2111..>2968
                     /locus_tag="VK103_01165"
     CDS             2111..>2968
                     /locus_tag="VK103_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLQ82117.1"
                     /translation="MGLYLGLANGDVPKDPYAFTPALARAWSERGVRCLAVGFRDVPQ
                     AETARAAVELRPLLTDHGIHISQYVGINAHLAHVDQARRADGIRRIREAVPAARALDA
                     TMMQSGVGTNAVAGLGTSPAPPPPGEPHHHDAPHHYAPHPDNYSPAARDGLVRALREV
                     ATMMADEGMVFSLECHQLTTMRSARVIRDVLDEVDSPAVVANFDPVNLLDSATAVYDN
                     AARMRAMADVLLPPDGVARLGPSCHVKDVVLRSGLVCHIDEEAPGAGVLDLSVLLELA
                     QRLPTGRREG"
BASE COUNT          448 a         1130 c         1005 g          385 t
ORIGIN      
        1 cttcgggatc gccgcccacg gaccggatgc cgacgtcgac tgcgagatcc agctgcaggg
       61 agcaccgcac agccatttcc gcttcctggc ccgcgccgcc gaacagctga tcatcgacgg
      121 cgcaccgccc tacccggggg ccgaacgcac cctgctgacc ggtggcatcc tcgaccacgc
      181 catgcgtcag gcccgcggcg ccgtcgaccc ggccacgccg cacctggacg tggcgtacac
      241 cgtgcccgcg cacatccgcg acaccggcgc gtaccttccg acgtaccagc accgggacaa
      301 ctgaccgccg tcacggagtg cacgacaccc tggccgttga ccatggatcg ctcagcgacg
      361 gatcatccgg gcgaggtcgt cggcagcgag ccggccgagc tcggtcgccg agaacacctg
      421 gtacgccgcg aggtcgacct ggctggcgaa gctctgtcgg gccagcaccg gggtgtagtc
      481 actgccccac accaggtgct ccaccccgac ccggtcccgc atgtgctgca ggtacggtcg
      541 cgccgacggg aacggtggcg tctcgtcacg gccggccacc gcgtacaggc cggacacctt
      601 cacccggacg tggccgatgg cccggaccgc cgccacgacg ggggcaagtc gttcggcggg
      661 ggtgccgctg gcccgtcccg gcagacccag gtgtgacagc agcaccgtgg tgtgctgcag
      721 ccgcgccaat cggggcgtca gcaccgccag cgcggcgggg tcgatgttga ggctccacgg
      781 caccggggcg tcgaggcctt cccagacctc gtcggacagg gcgtcccagg cgcgggcctc
      841 ctcggtgccg agcacgtacg cgctccaccc ggccgcaccg gccgtcagcc agtcccgggc
      901 gaggccgggg tcggctccga gccgctcgcg gtcgaggtgg accaggggca cgatccagtc
      961 gtgccggcgt gccagcgcac agacatggtc attgttgccg gcgaacctct gctggccctc
     1021 gtagccgacg accagcccgg ggccgatccc gaactgctgc cgcagccgga ggtaggcctg
     1081 cagctcggcg cccggctcgg aggtgccgtc gaacccacgc tcgaagaggt ggaggtgtgc
     1141 gtcgatcacc gcgtcaggac cggcccgcgc aggtgcgaca gcaacggcga ggcgagcatc
     1201 ccgatcgccc gtccgacatc ggccgccggc accatggacg ccttgaaccg ttcggccacc
     1261 gccgggtcgg cgccatgctc ctcaccggtg gccagggacc ggtgcaccag tggggtgtcg
     1321 acctgggcgg gcatcacgtt catcacccgg atcccgtgcc ccttcaggtg ttcggtgagg
     1381 gtgagcgcca gcccgttcac tgcgcccttg ctggccccgt acgggatgga gctgctcgac
     1441 ccgagcactc cggcaccact gccgatcagc acgatcagcc cggccccggc ggggaccatc
     1501 acccgggcga cggcgcgggc caccaggaag ctgccgacga cgttgacgtc cagcacggca
     1561 cggaaggact cctccgacag gtcgcgtacg tcggtgcggg cctctcgggt gatgccggcc
     1621 gcgtggatca gcacgtccag gccaccgagt gcctcggcca cacccgccac ggcggcctcg
     1681 acgccgtcgg cgtcggtcac gtcacaggtc gcggcgtggg ccacaccgag ctcggcgcac
     1741 cgggtggtgt cgcggtcgag tgccgccacc gccgcaccgt ggcgggccag gaagccactg
     1801 gtcgccaggc cgatcccgct ccccgccccc gtcaccagga cgcgccgtcc agccaacccg
     1861 agttcctcca tgatcccatt acggtcgtag tgtcgccggc gtgtcaatgg ggcgcgcccg
     1921 ccgatggacg acccagccgg gagcgctcac cccggaaccg ccgccacccc gaacccgcca
     1981 ccccgaaccc gctgccatcg aagcgttcca gcagactgac atccggggtt ggccagcccg
     2041 ggtcgccatg attcaatagg gtccgataaa agccaaacaa tcagtacgca ggcaactgcg
     2101 ggggtgctcg atgggtctgt atctcggcct cgcgaacggc gacgtgccca aggacccgta
     2161 cgcgttcacc cccgccctgg cccgcgcgtg gtccgagcga ggcgtccgat gcctggccgt
     2221 cggcttccgc gacgtgccgc aggccgagac cgcccgcgcg gccgtcgagc tgcgccccct
     2281 gctcaccgac cacggcatcc acatcagcca gtacgtcggc atcaacgccc acctcgccca
     2341 tgtcgatcag gcccgccggg ccgacggcat ccgccgcatc cgggaggccg tgccggccgc
     2401 tcgcgcgctc gacgccacga tgatgcagag cggggtcggc accaatgcgg tcgccgggct
     2461 cggcacgtca ccggctccac cgccgccggg cgagccgcac caccacgacg caccacacca
     2521 ctacgcacca cacccggaca actactcccc ggccgcccgg gacggcctgg tccgggcact
     2581 gcgcgaggtc gcgaccatga tggccgacga gggcatggtg ttctcgctgg agtgccacca
     2641 gctgaccacg atgcgcagcg cccgggtcat ccgcgacgtg ctggacgagg tcgactcccc
     2701 tgccgtggtg gcgaacttcg acccggtgaa cctgctggac tcggcgaccg cggtgtacga
     2761 caacgcggcg cggatgcgtg cgatggccga cgtcctgctg ccacccgacg gcgttgcgcg
     2821 cctcggcccc agttgccacg tcaaggacgt cgtgctgcgg tccgggctgg tgtgccacat
     2881 cgacgaggaa gcaccgggcg ccggcgtgct cgacctgtcg gtgttgttgg agctggcgca
     2941 acggttgccg accggacgtc gcgagggg
//
LOCUS       DATGUK010000075         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1132859,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000075 DATGUK010000000
VERSION     DATGUK010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132859"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2076
                     /locus_tag="VK103_01170"
     CDS             <1..2076
                     /locus_tag="VK103_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HLQ82118.1"
                     /translation="QLHGSVVGRLAEERAVVAAEVRVDGDPHRIDGRHGAMVLVPARV
                     QRLEGRGHRWATAVPVLLVADADSSLRHATVGSRWRLTTQLSSPDPGDSVAAFADVLD
                     ADLLEPPHPGWQLVERVRDGLRRSVAERPPGPRALVPALVVGDISAMPPHLQELFRTT
                     GLTHLTAVSGANLTLLLGFVLVMVRWLGVRGRWLTVTGLLTVAVFVALCRTEPSVLRA
                     TAMGLVALAAVGHGGTTGTGGRGLRHLCIAVIALLVVDPWLGRSVGFALSTLATAGIL
                     VWAGGWARRLCWTPRVVAEGIAVPLAAQLATQPVVSAISGQLSLVGLLANALAGPLVG
                     PATVAGFAAAGLSQLHPLLGSVAGWLASWPAQGIIWIAELTGALPGAAVRVPATPVAV
                     AGLAVAVPVAAWWTSRMLSRPWACGAIALTLVVVLLRAPAPPGWPPAQWSLVMCDVGQ
                     GDAAVVRIAPRTAIVVDTGPDPADLTRCLDQLGIRRTPMVVLTHYHADHVGGLGALLD
                     DHGVDLVLTSPTPVPADTARAVTAALDARGIRHRPSTAGDSITIGATRWETLWPTRAA
                     ARGTWAINDTSVVGRLTAQDGMTVLFTGDLEPTGQASLLGLGTDLRAMVLKVPHHGSA
                     DQDPGFLAAVGARWAITSAGEDNSYGHPSPRTIRALHGLGMGVLRTDTSGAVAIGADD
                     GRWVVRTQR"
     gene            complement(2083..>2686)
                     /locus_tag="VK103_01175"
     CDS             complement(2083..>2686)
                     /locus_tag="VK103_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HLQ82119.1"
                     /translation="PTVHLLHDAHHRLTPIEAGRLGAALEPYRMFWLEDPTPAEDQTA
                     FRIIRQHTTTPLAVGEVFNTVWDCHELITNRWIDYIRCAVSHAGGITHLRRIYALAET
                     YGVRTGAHGPSDVSPIGHAATLHLDLAVPNFGVQERMQHPAGTEEVFRTGISFDDGYL
                     HPGTAPGLGVVFDEEAAARFPYQRRYLPVNRRLDGSVHDW"
BASE COUNT          334 a          969 c          976 g          407 t
ORIGIN      
        1 caactgcacg ggtcggtggt gggccgactg gccgaggaac gcgccgtggt ggccgccgag
       61 gtccgcgtcg acggggaccc gcaccgcatc gacggtcgtc acggtgccat ggtgctggtg
      121 ccggcccggg tgcagcggct ggagggacgg gggcaccggt gggccaccgc agtcccggtg
      181 ctgctggtcg ccgatgccga ctcgtcgttg cgccacgcga ccgtcggatc gcggtggcga
      241 ctcaccaccc agttgtcctc gccggacccc ggcgactcag ttgccgcctt cgccgacgtc
      301 ctcgacgccg acctgctgga gccaccgcat ccgggttggc agttggtcga acgggtccgc
      361 gacggtttgc gtcgatccgt cgccgagcgg ccgcccggac ctcgtgcgct ggtgccggcc
      421 ctggtcgtcg gcgacatctc cgcgatgccc ccgcacttgc aggaactgtt ccgcaccacc
      481 ggcctcaccc acctcaccgc ggtgtcaggg gcgaacctca cgctgctgct cgggttcgtg
      541 ctggtgatgg tgcgctggct cggcgtccgc ggtcgttggc tgaccgtcac cgggctgctg
      601 acggtcgcgg tgttcgtggc gctgtgccgc accgagccca gcgtgctgcg cgccaccgcg
      661 atggggctgg tcgcgcttgc tgccgtcggc cacggcggga ccaccggcac cgggggacga
      721 ggcctgcgtc atctgtgcat cgcggtcatc gcgctgctgg tggtcgaccc gtggctgggc
      781 cgttcggtcg gattcgcgct gtcgaccctc gcgacggccg ggatcctggt gtgggcgggt
      841 ggatgggcac gacggctgtg ctggacgccg cgagtggtcg cggagggcat cgccgtaccc
      901 ctggctgccc aactcgccac ccagcccgtg gtgtcagcca tctcgggcca gctcagcctg
      961 gtcgggctgc tcgccaacgc gctggccggc ccgctggtcg gcccggccac cgtcgccggg
     1021 ttcgccgctg ctgggctgtc ccagctccac ccgctgctcg gcagcgtcgc cggctggctg
     1081 gcgagctggc cggcccaggg catcatctgg atcgccgagc tgaccggggc gctgccgggg
     1141 gcggcggtgc gggtcccggc caccccggtc gccgtcgccg gcctggcggt cgcggtcccg
     1201 gtcgcggcat ggtggacgtc ccggatgctg tcacggccgt gggcatgcgg tgcgatcgca
     1261 ctcacactcg tcgtggtgct gctgcgggca cccgccccac ccggatggcc accagcacaa
     1321 tggagcctgg tgatgtgcga cgtcgggcag ggcgatgcgg cggtggtccg catcgctccg
     1381 cggaccgcga tcgtcgtcga caccggcccg gacccggccg acctcacccg atgcctcgac
     1441 cagctcggca tccgacgcac acccatggtg gtgctcaccc attaccacgc cgaccacgtg
     1501 ggtggcctcg gggccctgct cgacgatcat ggagtcgacc tggtgctcac ctcaccgaca
     1561 ccggtcccgg ccgacacggc ccgggccgtc accgcagccc tcgacgcccg cggcatccgg
     1621 caccgtccga gcactgcagg ggactccatc accatcggcg ccacccggtg ggagacactg
     1681 tggccgaccc gggcggcggc ccggggcacg tgggcgatca acgacacctc ggtcgtcggt
     1741 cggttgacgg cccaggacgg gatgacggtg ctgttcaccg gcgacctcga gcccaccggg
     1801 caggccagcc tgctcggact gggcaccgac ctgcgagcaa tggtgctcaa ggtgccgcac
     1861 cacggctccg ccgaccagga tcccggcttc ctggccgcgg tgggcgcacg ctgggcgatc
     1921 acctccgcgg gcgaggacaa cagttacggc catccgtccc cgcgcaccat ccgcgccctg
     1981 cacggtctcg ggatgggcgt gctgcgtacc gacacctccg gtgccgtcgc catcggtgcc
     2041 gacgacggtc gatgggtggt gcgcacccaa cggtgagcgg cctcaccagt cgtgcaccga
     2101 gccgtccaac cgccggttca ccggcaggta gcgccgctgg tacgggaaac gggccgcggc
     2161 ctcctcgtcg aagacgacgc ccagccccgg ggccgttccc ggatgcaggt agccgtcgtc
     2221 gaagctgatg ccggtgcgga acacctcctc ggtgccggca gggtgctgca tccgttcctg
     2281 cacaccgaag ttgggcaccg cgaggtcgag gtgcagcgtg gccgcgtgcc cgatcgggga
     2341 gacatcgctc gggccgtggg caccggtgcg caccccgtag gtctcggcca gggcgtagat
     2401 ccggcgcagg tgggtgatgc cgccggcgtg cgagaccgcg cagcggatgt agtcgatcca
     2461 ccggttggtg atcagctcgt ggcagtccca gacggtgttg aacacctcac ccactgcgag
     2521 gggggtggtg gtgtgctgcc ggatgatccg gaaggccgtc tggtcctcgg ccggggtcgg
     2581 gtcctccaac cagaacatcc ggtacggctc gagcgcggca cccaaccgac cggcctcgat
     2641 cggggtcagc cggtgatgag catcgtgcag caggtgcacc gtcgga
//
LOCUS       DATGUK010000076         4504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_432522,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000076 DATGUK010000000
VERSION     DATGUK010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432522"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VK103_01180"
     CDS             complement(<1..217)
                     /locus_tag="VK103_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HLQ82120.1"
                     /translation="MVDAERILDGLDPEQRAVATALHGPVVVLAGAGTGKTRAITHRI
                     AYGVATGTYDPRQVLAVTFTTRAAGELR"
     gene            complement(302..1174)
                     /locus_tag="VK103_01185"
     CDS             complement(302..1174)
                     /locus_tag="VK103_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HLQ82121.1"
                     /translation="MATMIVVGMVVLAVTATGLGVLIGRNWPETRPTVTGQPPATTPA
                     APPSPAGSPSPTGTRPSPSASASSGGDVYADLPQVVANPVYDLAPRPLECDDLQLPVP
                     EGDRLERWSEQAMRCLQEHYAPVVAEAGATLTTPHLTFYDDEVDTPCGDDATGAFYCA
                     IDEGIYVERGVYVREAGRLNTIRMLLHEYSHHVQNRVGILGDDKYGREPVPVISRRVE
                     LQASCWTGMQLRGSQWLGWDEQDEQWLERFNSVDSDELHGEAASRWYWYEQGRTSSTF
                     AACNTWLADRDRVS"
     gene            1618..>4504
                     /gene="recC"
                     /locus_tag="VK103_01190"
     CDS             1618..>4504
                     /gene="recC"
                     /locus_tag="VK103_01190"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HLQ82122.1"
                     /translation="MTPPHLHLHRAVRSSDLIDGLAALVSEQPLDPFTSEIVSVPTPG
                     VERWLGQALSTRLGVAAGIDFLPFGRWMDQVWATASAPLLPPGEDGDPWSRDRLTRSV
                     LQVLDASAGEEWAAPVSRHVGLAAPGPDDPTVGHADVATGPGGRRFLTARRLAGLFHR
                     YHRERPAMVTAWLAGEDVDAAGAPLDRRHRWQAVLWRRVRTAVGLPSPAERIDAVRTH
                     LLDDPDRFALPARLSVFGLNRLDPLDRAVLQALATTREVHLWLAHHSPGRWDRIAAAP
                     SGELWPTRATHHRAAATVVTTAGNPLVSQLGRDVIEFQHVVSHLEVPATETVHGALPP
                     PTGVLGHLQHAIAADRPVSDTPVAVLQPGDDSLQVHSCHGPDRQVEVLREVICGLLTD
                     RPDLEPRDIMVMCPDLPRFAPLVEAAFDPTGTLAAHPDPRLHPGRRLRIRIADQSLTQ
                     VNPVLGVLERVLDLVNARLTASDLVDLCASGPVAARFGFSADDLERLEHLVRSAGVRW
                     GLGAADRARFGLGGYGQNTVSAGLDRMLLGVAWSDTDHRHLGTVLPLDEVESSDVDLV
                     GRLAELVDRLGHTLRSLTGPHTVDAWIAALQGAVESLTAAEPGEAWQLGHAWSSLQTL
                     RHADDDVRLPPGGTDADAPAHGDDGPLLDLGDLRAAVAEVLAGRPSRANFRTGSLTLS
                     SLAPMRSVPHKVICLLGMDDQAFPRGHTRDGDDLLAAEPRVGDRDRRSEDRQLLLDAI
                     MAAEDTVVVVHSGRDPRTNSVRPPAVPVGELLDTVAALVAADDGGDVRDQLVVEHPLQ
                     PYDARNFGPDTEVRSFDRTALAAARAASGIRTEPTPVFTRSPLPPVPPDPDRSVGLAD
                     LQGFFKHPVRAFLRERGRWTAAREEDDDIDLIPLDLDGLQTWKIGDRLLAARRAGADE
                     ATLAAAEWRRGVLPPKKLGGRVLDSVLEAVDQIHTSAERWRGQPRREV"
BASE COUNT          541 a         1704 c         1648 g          611 t
ORIGIN      
        1 gccgcagctc cccggcggcg cgggtggtga acgtcaccgc cagcacctgg cgcgggtcgt
       61 aggtgccggt cgcgacgccg tacgcgatcc ggtgggtgat ggcgcgggtc ttgccggtgc
      121 cggcgccggc cagcaccacg accgggccgt gcagggcggt ggcgacggcc cgctgctcgg
      181 ggtcgagccc gtccaggatc cgttccgcgt ccaccacggg gctcacccta accggcggca
      241 ccgaccgcgg gacgtgccgg agcccggcac cgggcagcgg acaccgggtc aggacaccgg
      301 gtcaggacac tcggtcccga tcggcgagcc aggtgttgca ggcggcgaac gtgctgctgg
      361 tccggccctg ttcgtaccag taccaccgtg aggccgcctc gccgtgcagc tcgtcggagt
      421 cgacgctgtt gaaccgttcc agccactgct cgtcctgctc gtcccagccc agccactgcg
      481 acccgcgcag ctgcatcccg gtccagcagc tggcctgcag ctcgacccgg cgcgagatca
      541 ccggcaccgg ttcgcgtccg tacttgtcgt cgccgaggat gcccacccgg ttctggacgt
      601 ggtgggagta ctcgtgcaac agcatccgga tggtgttgag ccggccggcc tcccgcacgt
      661 acacgccgcg ttccacgtag atcccctcgt cgatcgcgca gtagaacgcc ccggtcgcgt
      721 cgtccccgca cggggtgtcc acctcatcgt cgtagaaggt cagatgcggt gtggtgagag
      781 tggcaccggc ctcggcgacc accggcgcgt agtgctcctg caggcaccgc atcgcctgct
      841 ccgaccagcg ttccaaccgg tcgccctccg ggaccggcag ttgcaggtcg tcgcactcca
      901 gcgggcgtgg cgccagatcg tacaccgggt tcgccacgac ctgtggcagg tcggcgtaga
      961 cgtcgccacc ggaggaggca ctcgccgacg gggaggggcg cgtcccggtc gggctcgggg
     1021 agcccgccgg tgaggggggc gccgccgggg tcgtggccgg tggctggccg gtcacggtcg
     1081 gccgggtctc cggccagttc cgcccgatca gcacgccgag cccggtcgcg gtgacggcaa
     1141 gcacgaccat gcccaccacg atcatcgtgg ccatcaccgg cgcgcccgac cgcgccgggg
     1201 tgggtggcgg ctccgacggg cgatgggcgg ccgggtgcgt gccgggtggt gccggcggcc
     1261 aggacggccg cgctccgtac gggggtcccg gtcggtgcgg gggtcccggt cggcccgggg
     1321 gtcccggtcg gtgaggtggc ggaggcggcc cgggcggctg gtacggcgac cggacgggct
     1381 ggtggtgcgg cgccggcggg ccgggcagcg gtgggccggg cgccggtggt gcggagggct
     1441 gcggacccgg ctgggaccgc ccgggggttg cgggcacacc tgcacccgcc gcgtccgact
     1501 gctcggtcgg ccgcgcccag gactcctgcg gatccatctc ggcccgcccc tttcgctgac
     1561 cccagcgtac ggtgccaccc ggccgtgtcg gagggtggtt ctagggtgat ccccacgatg
     1621 acaccaccgc acctccacct gcaccgggcc gtccgatcca gcgacctgat cgacggcctg
     1681 gctgcgctgg tgtcggagca gccgctcgac ccgttcacct ccgagatcgt cagcgttccc
     1741 acgccggggg tcgaacggtg gctcgggcag gcgctctcga cccggctcgg cgtggctgcc
     1801 gggatcgact tcctcccgtt cggtcgttgg atggaccagg tctgggccac cgcctccgcc
     1861 ccgctgctgc cgccgggcga ggacggcgac ccgtggagcc gcgaccggct cacccggtcg
     1921 gtgctgcagg tgctggacgc ctccgcgggt gaggagtggg cagcgccggt cagccggcac
     1981 gtgggcctgg ccgcacccgg ccccgacgac cccaccgtcg ggcatgcgga cgtcgcgacc
     2041 ggcccgggtg gtcgtcggtt cctcaccgcc cgccggctcg ccgggctgtt ccaccgctac
     2101 caccgggaac ggccggccat ggtgacggcc tggctggccg gggaggacgt tgatgccgcc
     2161 ggcgcgccgc tggaccgccg gcaccggtgg caggccgtgc tgtggcgccg ggtgcgcacg
     2221 gccgtcggcc tcccctcccc ggcggagcgg atcgacgcgg tgcgcaccca cctgctcgac
     2281 gaccccgacc ggttcgcgct gcccgcccgg ctctcggtgt tcgggctgaa ccggctcgac
     2341 ccgctcgacc gggccgtgct gcaggcgctg gccaccaccc gggaggtgca cctgtggttg
     2401 gcccaccact caccggggcg atgggaccgg atcgccgccg caccctccgg cgagctgtgg
     2461 ccgacccgag ccacccacca ccgcgcggcg gccacggtcg tcaccacggc tggcaacccg
     2521 ctggtcagcc agctcgggcg ggacgtcatc gagttccagc acgtcgtcag ccacctggag
     2581 gtgccggcca ccgagaccgt ccacggtgcg ctgcccccac cgaccggcgt gctcggccac
     2641 ctgcagcacg cgatcgcggc cgaccgtccg gtctccgaca ccccggtcgc cgtgctgcaa
     2701 cccggtgacg acagcctgca ggtccactcc tgccacggtc ccgaccggca ggtggaggtg
     2761 ctgcgcgagg tgatctgcgg actgctcacc gaccgtcccg acctggagcc acgcgacatc
     2821 atggtgatgt gccccgacct gccgcggttc gcacccctgg tggaggccgc cttcgacccg
     2881 accggcaccc tcgccgccca ccccgatccc cgactgcatc cgggccgccg gttgcggatc
     2941 cggatcgccg accagtccct cacccaggtc aacccggtcc tcggtgtcct ggagcgcgtg
     3001 ctcgacctgg tcaacgcccg gctcaccgcc tccgacctgg tcgacctgtg cgccagcggc
     3061 ccggtggcgg cccggttcgg cttcagcgcc gacgacctgg agcgtctgga gcacctggtg
     3121 cgctcggcgg gggtccggtg ggggttgggt gccgcggacc gggcacgctt cgggctcggc
     3181 ggctacggcc agaacacggt gtcggccggt ctggaccgga tgttgctcgg cgtcgcctgg
     3241 tccgacaccg accaccgaca cctgggtacg gtgctgccgc tcgacgaggt ggagtcctcc
     3301 gacgtcgacc tcgtcggccg actcgccgag ctggtcgacc ggctcgggca caccctgcgg
     3361 tcattgaccg gcccgcacac cgtggacgcc tggatcgccg ccctgcaggg cgcagtcgag
     3421 tccctgaccg ccgccgagcc cggtgaggcg tggcagctcg gccatgcctg gtccagcctg
     3481 cagacgctgc ggcacgccga cgacgatgtc cggctgccgc ccggcggcac ggacgccgat
     3541 gccccggccc acggcgacga cgggccgttg ctggacctgg gcgacctgcg ggccgcggtc
     3601 gccgaggtgc tggccggccg accgagccgg gccaatttcc gtaccggcag cctcaccctg
     3661 tcctcgctgg caccgatgcg gtcggtcccc cacaaggtga tctgcctgct ggggatggac
     3721 gaccaggcgt tcccacgggg ccacacccgt gacggggacg acctgctcgc cgccgagccc
     3781 cgtgtcggtg accgggaccg gcggtccgag gaccgccagc tgctgctcga cgcgatcatg
     3841 gccgccgagg acaccgtggt ggtggtgcac tcgggccgtg acccgcggac caactcggtg
     3901 cgccccccag ccgtcccggt cggcgagctg ctggacaccg tcgccgcgct ggtggccgcc
     3961 gacgacggcg gcgacgtccg cgaccagctg gtggtggagc acccgctgca gccgtacgac
     4021 gcccgcaact tcggcccgga caccgaggtg cggtcattcg accggaccgc actggccgcc
     4081 gcgcgggcgg ccagcggcat ccggaccgag cccaccccgg tgttcacccg cagcccactc
     4141 cccccggtgc caccggaccc ggaccgatcg gtcgggttgg ccgacctgca gggcttcttc
     4201 aagcacccgg tccgggcgtt cctgcgggaa cggggtcggt ggaccgctgc ccgggaggag
     4261 gacgacgaca tcgacctgat cccgctcgac ctcgacgggt tgcagacctg gaagatcggt
     4321 gaccggctgc tcgccgcccg tcgcgccggg gccgacgagg cgacgctggc cgcggccgag
     4381 tggcgacgcg gggtgttgcc cccgaagaag ctcggcggca gggtcctgga ctccgtgctg
     4441 gaggccgtcg atcagatcca caccagtgcc gagcgctggc gcggccagcc tcgccgcgag
     4501 gtcg
//
LOCUS       DATGUK010000077         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_432790,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000077 DATGUK010000000
VERSION     DATGUK010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432790"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VK103_01195"
     CDS             <1..592
                     /locus_tag="VK103_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HLQ82123.1"
                     /translation="DLAPDAALSDVVDALADMDARSIHETARLIDLDGLEALIDAVSG
                     AGHVYAMGNGSAHAAAVYLELKLRSLGVATSTFRDMASAIIGISLARPGDVVVVVTPT
                     GIARESVPMLTEANRRGCVTVAITGNSRSAAAEIAQHCLVTVQAESRLRTGVLSSMIA
                     SLFLSDCISSGLVIRRHDRSVSALAAVDEAMRNYYW"
     gene            complement(641..1774)
                     /locus_tag="VK103_01200"
     CDS             complement(641..1774)
                     /locus_tag="VK103_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010839918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HLQ82124.1"
                     /translation="MRSLAALYVDAGYLLHAAATRVAGTSLRSSVKVDYAGLIKGLAD
                     QAAEMSGLPVLRTNWYDSGAGVRSVPDSVQVGIGMLPRVKLRLGRLSPNGDQKGVDVR
                     MGLDLVTHARNNAVDVMYLLSGDDDLTEAVEEAQSHGVQVSLLAVPDRDGHAFGVSLH
                     LRQEADGLELINPDVIDEHVTVAGHATTHPTGQPTAPDRPGPAVGPDGVAVSPPTPAT
                     VRRPGEPAAPRPPLPSVPPVPRVPTPEPTDPAAPSAPAPSAPEPAIPTPRVLATRSLR
                     PEPVYRTATGQTPVNHLAEVDTEELTETVDGVVRQALGAWQLTNTPTEVRQLLQDRPN
                     IPQALDRALLNDLSTALGIYELDEPTRFLLRRRFWELAQIQLA"
     gene            complement(1879..>2291)
                     /locus_tag="VK103_01205"
     CDS             complement(1879..>2291)
                     /locus_tag="VK103_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4191 family protein"
                     /protein_id="HLQ82125.1"
                     /translation="SMLPKTWTSAAAIAANKQMDVVHRALGPGGLILIGEGEPGRTRQ
                     LLASEARKHQRVTDRVEVVTLHMGDREGQVPLNKLTRHIQKLPKTLQNYEITEIKQRL
                     RALDAVRPRMPVPKGPMPTSARQMKGARHSLRGR"
BASE COUNT          304 a          783 c          825 g          379 t
ORIGIN      
        1 cgacctcgcg cccgatgccg cgttgagcga tgtggtcgac gcactcgccg acatggacgc
       61 ccgctccatc cacgagaccg cccggctgat cgacctggac ggcctggagg cgctgatcga
      121 cgcggtgagt ggggccggcc acgtctatgc gatgggcaac ggatcggccc atgccgccgc
      181 ggtctacctg gagctcaagc tgcgcagcct cggggtggcg acctcgacct tccgcgacat
      241 ggcgagcgcg atcatcggca tctcgctggc ccgacccggc gacgtggtgg tggtcgtgac
      301 gccgaccggg atcgcccggg agtcggtgcc gatgctgacc gaggccaacc gacgcggttg
      361 cgtcaccgtg gccatcaccg gcaactcccg gtccgcggcc gccgagatcg cgcagcactg
      421 cctggtgacg gtccaggccg agtcgcggct gcggacggga gtgctgagca gcatgatcgc
      481 ctcgctgttc ctcagcgact gcatcagcag tggattggtg atccggcgcc atgaccgttc
      541 ggtctccgcc ctcgcggcgg tcgacgaggc gatgcgcaac tactactggt gagtcgtgcg
      601 tacgggccgg cgacggccgg ccgtcagtcg tcccatgccc tcaggccagc tggatctgcg
      661 cgagctccca gaaccgacgg cgcagcagga accgggtcgg ctcgtccaat tcgtagatgc
      721 ccagcgcggt cgacaggtca ttcagcagag cgcggtccag cgcctgcggg atgttgggcc
      781 ggtcctgcag cagctggcgc acctccgtcg gggtgttggt cagctgccaa gcgcccagtg
      841 cctgccggac cacaccgtcc accgtctcgg tgagctcctc ggtgtcgacc tcggcgaggt
      901 ggttgaccgg cgtctgtccg gtcgcggtgc ggtagacggg ctcgggtcgc aacgagcggg
      961 tcgccaacac ccgtggggtc gggatggccg gctcgggggc gctcggcgcc ggggcggagg
     1021 gggcggccgg gtccgtgggt tcaggggtcg gcacccgagg tacgggtggt accgatggca
     1081 gtggtgggcg tggcgcggcc ggctcgccgg ggcggcggac cgtcgccggt gttggtgggg
     1141 agaccgccac gccgtccggt cccactgccg gtccggggcg atccggtgcc gtcggctggc
     1201 cggtcggatg agtcgtcgca tgcccggcga ccgtgacgtg ttcgtcgatg acgtcggggt
     1261 tgatcagctc caggccgtcg gcctcctggc gcagatgcag gctgaccccg aacgcatggc
     1321 cgtcccggtc cggtaccgcg agcaggctga cctgcacgcc gtggctctgt gcctcctcga
     1381 ccgcctcggt gaggtcgtcg tcaccggaca gcaggtacat cacgtcgacg gcgttgttcc
     1441 tggcgtgggt gaccagatcg agccccattc gtacgtccac gcccttctgg tcaccgttgg
     1501 gggacaaccg ccccagccgc aacttcaccc ggggcaacat cccgatgccg acctgcaccg
     1561 agtccggcac cgaacgcaca ccagcgcccg agtcgtacca gttggtgcgc agcacgggca
     1621 gtccgctcat ctcggccgcc tggtcggcca gtcccttgat caggccggcg tagtccacct
     1681 tgacgctgga gcgcaggctg gtgccggcca cccgggtggc tgcggcgtgc agcaggtaac
     1741 cggcgtcgac gtacaacgct gcaagggatc tcatcgccgt cagcgtagcc aactcggcgt
     1801 gctgatgtgg caggaatcac ctgatcggtc ggatcgccac ctccggtggc cgcccgggca
     1861 cccgttcagc agtggcggtc agcggccgcg cagcgagtgc cgggcgccct tcatctggcg
     1921 tgcactggtc ggcatcgggc ccttcggtac gggcatccgc gggcggaccg cgtccaacgc
     1981 ccggagtcgc tgcttgatct cggtgatctc gtagttctgc agcgtcttgg gcagtttctg
     2041 gatgtggcgg gtgagcttgt tgagcggcac ctggccttcc cggtcgccca tgtgcagggt
     2101 gaccacctcg acccggtcgg tgacgcgctg gtgcttgcgc gcctcgctgg cgagcagttg
     2161 ccgggtccgg cccggctcac cctcaccgat caggatcagc ccaccggggc cgagggcgcg
     2221 gtgcaccacg tccatctgct tgttggccgc gatcgcggca gcgctggtcc acgtcttcgg
     2281 cagcatcgac a
//
LOCUS       DATGUK010000078         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_900976,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000078 DATGUK010000000
VERSION     DATGUK010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900976"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1178)
                     /locus_tag="VK103_01210"
     CDS             complement(138..1178)
                     /locus_tag="VK103_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82126.1"
                     /translation="MPTYVLEGGSSITLPARPVSVRLQSSRAGAVRLEAIGVDGPVRS
                     INPAPGMLVVPHLPGPVTVRAVPVDDDRFESGSVLNLTVGYESRQDLDPDRGVLNGLD
                     VSGLRHRDVVMLEPQGDRIGVTVLGPAAAEEPLSPGAELARDAAREVLGTDQLPSRSA
                     VTAVVGIDVSASMRPLHADGTVAQVLDVLAGVHRVVGRPGRDSLRGCLVSRQARWLPP
                     APPERFTREVLQAAGELPLTTGFRAGAVLDAQRSGVPADGPVLHWLVTDGAPGDVAVL
                     RGRTDVHLVCPVPQSAGAVAIGQGLPTTVVGLPDVAGRPTTFGQDPTAVRQVVASLVG
                     PFLPSPQQAGAS"
     gene            complement(1233..>3235)
                     /locus_tag="VK103_01215"
     CDS             complement(1233..>3235)
                     /locus_tag="VK103_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82127.1"
                     /translation="IAPAHSHELFFSTLDRATGVAAVFARGVRLACIPRQDAEVAVRE
                     RDVSPRVVLATGDTVQLGDRDGAPHRTGAGDEVIVTAATGLVTAVLADPTAAVATLDR
                     ADAIAAEIGSTTSDTGPSDTGHPLWSLALAVAERGTVHADGLDHAAAVLTTGTPAAVL
                     TDPAQRERVERLVADHLGPTTADAWQACGSAAGTAVAGRLYLLRAVGDLDWLTRPEGV
                     QVPDGTTADAEVVERARGTATALRDMAAADPRTTAVATLRLAELTAAVGIDDPELTTV
                     LRTLAGGTVMAQLAGDTDGIIAAVGPIGEATRRLLVAVLEADRSWTAAPVGSRLPRPL
                     LRWLFPDGVDATLPTSTEVDQPTRLRAEVAAQLAAGLRSYRGLPAWVAFADDAVGDNV
                     RGWFAEPWPLDQLVRLQEAYGDRLGPDAWLATLKTTDDSAELQRVCERLGGTAWQTGQ
                     PPATATAALAGLRMLRPGWSRLGHRAAAVTTAINGAAAGQLVPDQPFATGTDRDVQLA
                     VILAIIDPSLVTDYGTEQRLESLRPLFEGQRLLVDTDLVNDVSAHLGTLDRNDFLARL
                     ARADPVFGHRLDGAAARIGWLEKVRTPTGPPTHGARVLRAALRAVLGDQPDRRELTTT
                     VREAVRRPGEDQRAVDKQVDGWVAALLGTAEPRRRGLFRGGT"
BASE COUNT          421 a         1209 c         1221 g          384 t
ORIGIN      
        1 cacgatccgc cgggaatggg tctgcacccc ggtgaagcgg gtcgcgacca ggtcggggtt
       61 gagctggcag cgctggttga cgcaccgcca ggcatgcagg tcggcggtga tgccgtggaa
      121 gcagtgcggg cacttggtca cgatgcacct gcctgctgcg gtgacggcag gaacggcccg
      181 accagcgagg cgacgacctg ccggaccgcg gtcgggtcct gcccgaaggt ggtgggtcga
      241 ccggccacgt ccggcagtcc gaccacggtg gtcggcaggc cctgcccgat cgcgaccgcc
      301 ccggccgact gcggcaccgg gcacaccagg tgcacatcgg tacggccccg cagcacggcc
      361 acgtcgccgg gcgcgccgtc ggtgaccagc cagtgcagca ccggaccgtc cgcgggcacc
      421 ccggaccgct gcgcgtcgag caccgcaccg gcccggaagc cggtggtcag cggcagctca
      481 ccggccgcct gcaggacctc ccgggtgaac cgttccggcg gggccggcgg caaccaccgg
      541 gcctgccgcg acaccaggca gccccgcagg ctgtcccgcc ccgggcggcc gacgacccgg
      601 tgcaccccgg ccagcacgtc gaggacctgc gccacggtgc cgtcggcgtg cagcggccgc
      661 atcgaggccg acacgtcgat gccgaccacc gcggtgaccg cggaccggct cggcaactgg
      721 tcggtgccca acacctcgcg ggccgcgtcg cgggccagct cggcgcccgg ggacagtggt
      781 tcctcggcgg cggccgggcc gagcacggtg accccgatcc ggtcgccctg cggttccagc
      841 atcaccacgt cgcggtgtcg gagaccggac acgtcgaggc cgttgagcac gccacggtcc
      901 gggtcgaggt cctggcgcga ctcgtagccg acggtgaggt tcagcaccga cccggactcg
      961 aaccggtcgt cgtcgacggg caccgcccgg acggtgaccg gaccgggcag gtgcggcacg
     1021 acgagcatgc cgggcgccgg gttgatgctg cgcaccgggc cgtcgacacc gatcgcctcc
     1081 aaccggaccg ccccggcccg gctggactgc agccggaccg acaccggcct ggccggcaac
     1141 gtgatcgacg aaccgccctc aagcacgtag gtgggcatcc cgactccttc ccctcacggt
     1201 ggccaccggc catccggtcc ccggtcgtgc catcacgtcc cgccccggaa cagtccacga
     1261 cgccgcggtt cggcggtccc cagcagggcg gcgacccagc cgtcgacctg cttgtcaacg
     1321 gcgcgctggt cctcacccgg gcgccggacc gcctctcgca cggtggtggt gagctcccgg
     1381 cggtccggtt ggtcgcccag cacggcacgc agcgcggccc gcaacacccg tgcgccgtgg
     1441 gtcggcggac cggtcggggt gcggaccttc tccagccacc cgatccgggc ggccgcgccg
     1501 tcgaggcggt gcccgaacac cgggtcggcg cgggccagcc gcgccaggaa gtcgttgcgg
     1561 tccagggtgc ccaggtgtgc gctgacgtcg ttgaccaggt cggtgtcgac cagcaaccgt
     1621 tgcccctcga acagcggccg caacgactcc agccgctgct cggtgccgta gtcggtcacc
     1681 agcgacgggt cgatgatcgc caggatcacg gccagttgca cgtcacggtc ggtgccggtg
     1741 gcgaacggct ggtccggcac cagctgcccg gccgcggccc cgttgatcgc cgtcgtcacc
     1801 gcagccgccc ggtgccccag ccgggaccag ccgggccgca gcatccgcag tccagccagc
     1861 gccgcggtgg ccgtcgccgg cggctggccg gtctgccacg ccgtaccgcc gaggcgttcg
     1921 cacacccgtt gcagctcggc ggagtcatcg gtcgtcttca gggtggccag ccaggcgtcc
     1981 gggccgagcc ggtccccgta cgcctcctgc agtcgcacca gctggtccag tggccacggc
     2041 tcggcgaacc agccccggac attgtccccg acggcgtcgt cggcgaacgc gacccaggcg
     2101 gggagcccgc ggtaggaccg cagcccggcg gcgagctgcg cggccacctc ggcccgcagc
     2161 cgggtcggct ggtccacctc cgtgctggtc ggcagcgtgg cgtcgacgcc gtcggggaac
     2221 agccagcgca gcagcggacg cggcagccgg ctgccgacag gtgcggcggt ccaggaccgg
     2281 tcggcctcca gcaccgcgac gagcaggcgg cgggtggcct ccccgatcgg gcccaccgca
     2341 gcgatgatgc cgtcggtgtc gccggcgagc tgggccatca cggtgccgcc ggcaagcgtg
     2401 cgcagcacgg tggtgagctc ggggtcgtcg atgccgacgg ccgcggtgag ctcggcgagc
     2461 cggagggtgg cgaccgcggt ggtacgggga tcggcggcgg ccatgtcgcg cagagcggtc
     2521 gcggtgccac gggcgcgttc gacgacctcg gcgtcggcgg tcgtgccgtc gggcacctgc
     2581 acgccctcgg gacgggtcag ccagtccagg tcaccgacgg cccgcagcag gtagagccga
     2641 ccggcgacgg cggtcccggc cgccgatccg caggcctgcc aggcatcggc ggtggtgggt
     2701 ccgagatggt cggcgaccaa ccgttcgacc cgttcccgct gcgccggatc ggtcagcacc
     2761 gccgcggggg tgccggtggt gaggacggcg gcggcatggt ccagaccgtc ggcgtggacg
     2821 gtgccgcgtt cggcgacggc cagggccagc gaccacaacg gatgcccggt gtcgctggga
     2881 ccggtgtcgc tggtggtgga cccgatctcg gcggcgatgg cgtcggcgcg gtccagggtg
     2941 gcgacggcgg ccgtcggatc ggccaggact gcggtgacca gcccggtcgc ggcagtcacg
     3001 atgacctcat cgccggcgcc ggtccggtgc ggggcgccgt cccggtcgcc gagctggacg
     3061 gtgtcgccgg tggccagcac cacccggggc gagacgtccc gttcccggac cgccacctcg
     3121 gcgtcctgcc gcgggatgca ggccagtcgg acgccacgcg cgaacaccgc cgccaccccg
     3181 gtggcccggt ccagggtgga gaagaacagc tcgtggctgt gcgcgggcgc gatca
//
LOCUS       DATGUK010000079         3346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_85893,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000079 DATGUK010000000
VERSION     DATGUK010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85893"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="VK103_01220"
     CDS             complement(<1..1403)
                     /locus_tag="VK103_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HLQ82128.1"
                     /translation="MKREALGVALLLLCAAGAMALLQGRSILGAVVWLVAYAAVVALT
                     ARAARRQRTLARRLGTYVTVRSVLVFGLAAPLVQQPVWTTWPAWGALLLVVAVIRCEP
                     RLGRSLRRHGVRTANLPGLQTPAALSGPATVLFLCQLAVLPALLVLGLVDAAPVFWLL
                     LVLPSVGAAVWAATVGLTRRWQIGNARTVLTTVLEAYRPRFAVYWDAPPGSGYQLAMW
                     LPYLRRVGEPFVVLVRNPAGFNEAAALVDDVPVVLTRTLADVERCVVPTLRAAFYVNN
                     AARNSHLVRFAALTHVQLLHGDSDKAPSYNPVTAMFDRIFVAGQAGIDRYGAHGVDIP
                     RERFAIVGRPQVESIELVDTTADRVPTTVLYAPTWRGQHTDAAYSSLEHGPALIEMLL
                     ARGCAVVFRPHPYSRRDAGHRALIEQIHERLASDTGPARSEHLFGPDATDISVNDCFN
                     RCDAMIADVSSVVVDFL"
     gene            1548..2072
                     /locus_tag="VK103_01225"
     CDS             1548..2072
                     /locus_tag="VK103_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLQ82129.1"
                     /translation="MYSPEPGAPSIERRSVVAQVRDRLRRDITSGRLVADTLYSVTAV
                     AAELGVSRTPVREALLQLAQLGLVQFHRNRGFTVTNVSADELIEIFQLRLAIETAAAR
                     RAATGATGTAELEAQLEAMATAAAEQDRPRFMVADRAFHECLLDIAGNRRAHDAVTVA
                     RDTLFSRGRIACYM"
     gene            2291..2566
                     /locus_tag="VK103_01230"
                     /pseudo
     CDS             2291..2566
                     /locus_tag="VK103_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2563..>3346
                     /locus_tag="VK103_01235"
     CDS             2563..>3346
                     /locus_tag="VK103_01235"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLQ82130.1"
                     /translation="MTMIDNHCHVFLADGITPPAPVPGAAYGAPPVGLAEHRAHLDAL
                     GCDRGVLVQPSAYGTSDHGVLLATLRRDPDRLRGVACVPVGTPDADLREMHRAGVRAT
                     RVQDGYPGGVPVTRIAEVAAMVQPFGWHIEIWSDVRRHMDWLAPVVRDLPVPVVIDHL
                     GHLPSDVPTSDPAVAMVTDLLADGHAWVTLSGLDRLLPDTIDPEGAEFADRWREHEDR
                     IADRVAAFVAARPDQVMWGTDWPHVGLDLPHPSVQDLLQRLHR"
BASE COUNT          531 a         1202 c         1127 g          486 t
ORIGIN      
        1 tgcaggaaat cgaccaccac gctggagacg tcggcgatca tcgcgtcgca gcggttgaag
       61 cagtcgttca ccgatatgtc ggtcgcatcc ggtccgaaca ggtgctccga ccgggccgga
      121 ccggtgtcgc tggcgaggcg ttcatggatc tgctcgatga gggcccggtg cccggcatcg
      181 cgtcggctgt acggatgcgg tcggaacacc acggcgcagc cacgcgccag caacatctcg
      241 atcagcgccg gcccgtgctc cagcgagctg taggccgcat cagtgtgctg cccgcgccac
      301 gtcggcgcgt acagcacggt ggtcggcacc cggtcggcgg tggtgtcgac gagttcgatc
      361 gactccacct gggggcgtcc gacgatggcg aaccgctccc gcgggatgtc gaccccgtgc
      421 gcgccgtacc ggtcgatgcc ggcctgcccg gcaacgaaga tgcggtcgaa catcgcggtc
      481 accgggttgt agctgggcgc cttgtcgctg tcaccgtgca acagctggac gtgggtcagt
      541 gcggcgaagc ggaccagatg gctgttgcgc gccgcgttgt tgacgtagaa cgcggcccgc
      601 agcgtcggca ccacgcaccg ctccacgtcg gccagcgtac gggtgaggac cacggggacg
      661 tcgtcgacga gtgcggcggc ctcgttgaag ccggccgggt tgcggaccag caccacgaac
      721 ggctccccca cccggcgcag gtagggcagc cacatcgcca gctggtaacc ggatccggga
      781 ggggcgtccc agtacaccgc gaaccgcggt cggtacgcct cgaggacggt ggtgagcacc
      841 gtccgggcgt tcccgatctg ccaccgtcgg gtcagcccga ccgtggcggc ccacaccgcc
      901 gcgcccacgg agggcagtac cagcagcaac cagaagaccg gcgcggcgtc gaccaggccc
      961 agcaccaaca gcgccggcag caccgccagc tggcagagga acagcacggt tgccgggccc
     1021 gacagggccg ccggagtctg cagccccggg agattggcgg tccggacccc atgtcggcgc
     1081 aatgaccggc ccaaccgggg ctcgcagcgg atgaccgcga cgaccagcaa cagcgccccc
     1141 caggctggcc aggtcgtcca caccggctgc tgcaccaggg gtgcggcgag cccgaagacc
     1201 aacaccgaac ggaccgtcac ataggtaccg aggcgacgcg ccagtgtccg ttgtcgacgg
     1261 gccgcccgcg cggtgagcgc gaccaccgcc gcgtaggcca ccagccacac caccgccccg
     1321 aggatggagc gcccctggag cagtgccatt gcaccggcgg cgcacagcaa cagcagtgcg
     1381 actcccagag cctctcgttt caatgcatgc tcccgcacca ggtcgaaccg gtcatgacgt
     1441 tcgatctaac cacagctgac cggccgggaa acgcaggaca ccacgccgac ccgccaccca
     1501 cacacatccg gagccccggc ccgtctcggg tgcggcacaa tggcgccgtg tactccccgg
     1561 aacccggcgc tccgagcatc gagcgacgca gcgtcgtcgc ccaggtgcgg gaccggctgc
     1621 gacgtgacat cacctccggt cgactggtgg ccgacacgct gtactcggtg actgcggtcg
     1681 cggcggagct cggggtctcc cgcaccccgg tgcgggaggc actgctgcag ttggcccagc
     1741 tcggactggt gcagttccac cgcaaccgcg gcttcaccgt gaccaatgtc tcggccgacg
     1801 agctcatcga gatcttccaa ctgcggttgg cgatcgagac cgcggcagcc cgacgggcag
     1861 caaccggcgc caccgggact gccgagttgg aggctcagct cgaagcgatg gccacggcag
     1921 cggccgagca ggaccggcca cgattcatgg tcgccgaccg tgcgttccat gagtgcctgc
     1981 tggacatcgc cggaaatcga cgcgcccacg acgcggtgac cgtcgcgcgc gacacactgt
     2041 tcagtcgcgg gcgtattgca tgttacatgt gaggagcggc gaccatgacc tcgacagacg
     2101 cgccggacac cgaccgacgg tcctggtacg gacggatgac cggcaaggaa cggcacacct
     2161 tctggggttg cttcggaggc tgggccctgg atgccttcga cgtgcagctc tacagcctgg
     2221 tgatcccggg gctgctggca ctgcacttcg tccgcgactc gggtgaggcc ccaacggtgt
     2281 gttcatcgcg ttgtgcttcc cactcggctt cgtctcctct gcatacttca gcggcatcgg
     2341 tgcgttcttc accgagcagt tcccgaccca actgcgcggc tccggccagg gcttcgcata
     2401 caacttcggt cgcgggatcg gggccctgtt cccgttcctg gtcgggtcgc tggccgaggc
     2461 catgagcatc ggccccgcga tcgcactgtt cgccgtcgtg gcgtacggcg tgctcgcgct
     2521 cgccgcactg ctgctccccg aaacccgggg acaggagctg gtatgaccat gatcgacaac
     2581 cactgccatg tgttcctggc cgacgggatc accccgcctg ctccggtgcc cggggccgcg
     2641 tacggcgcac caccggtcgg actcgccgag caccgtgccc acctcgatgc actcgggtgc
     2701 gaccgcggtg tgctcgtgca accgagtgcc tacggcacct ccgaccacgg cgtgctgctc
     2761 gcgacgctgc gccgggaccc ggaccggttg cgcggggtcg cctgcgtccc ggtcggcacc
     2821 ccggacgccg atctccgtga gatgcaccgg gccggggtgc gagccacccg ggtgcaggac
     2881 ggctaccccg gcggcgtccc ggtcacccgg atcgccgagg tggctgccat ggtgcagccg
     2941 ttcggctggc acatcgagat ctggagcgat gtacggcgac acatggactg gctggccccg
     3001 gtcgtccgcg acctccctgt gccggtggtg atcgaccacc tcggtcacct gcccagtgac
     3061 gtgccgacca gcgatccggc cgtggcgatg gtcaccgatc tgctcgcaga cggtcacgca
     3121 tgggtcaccc tgtccggact ggaccgactg ttgcccgaca ccatcgaccc cgagggtgcc
     3181 gaattcgccg accgctggcg ggagcacgag gatcgcatcg ccgaccgcgt cgccgccttc
     3241 gtcgccgccc ggccggacca ggtgatgtgg ggcacggact ggccccacgt cggcctggac
     3301 ctgccccacc cgagtgtgca ggacctgttg caacgcctcc atcgct
//
LOCUS       DATGUK010000080         4803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_673108,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000080 DATGUK010000000
VERSION     DATGUK010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673108"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..820)
                     /locus_tag="VK103_01240"
     CDS             complement(134..820)
                     /locus_tag="VK103_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03089 family protein"
                     /protein_id="HLQ82131.1"
                     /translation="MTFLDLLQRRIRRDGARPLLTCYDADGARTELSATTYGNWVAKS
                     ANLLAWTLDADTGDRIALPVAVRHPAHWMTLVWVAACWTVGVEVVLAPDPDTVAVVTG
                     PDLEFGDNAAQRLACSLHPLGLGFASPLPDDVTDWAEVVKGEDDDYVGSEPTPDDPVW
                     EGRALAEVLDVAPTADRVLVDGRAPLDPLAVARAVLVAPLLGGGSAVTVLGGDPAAVA
                     TGEHAIVVGM"
     gene            877..1773
                     /locus_tag="VK103_01245"
     CDS             877..1773
                     /locus_tag="VK103_01245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014986839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLQ82132.1"
                     /translation="MQLRLPGRTHQQWFADNAANWDDRARVHATSSGYGLDDYVTDPD
                     KLSHTVRYDSADLGDLTGVRGVHLQCHLGTDTISLARLGATMTGVDLSPESVAIANRL
                     AARTGDAADFVAANVYDAPAALAAAGHPPGSFDLVYTGGGALCWLPDIAAWARVCAEL
                     LAPGGRLHVRDFHPMMLALADERPDGLLVVEHPYFETAEPQTWDDGATYVAGGDDAPD
                     ITATVSHEWNHGLAETITAVLDAGLQLTLVREDRTSGWARFPTAMVDAPGLEHEYELA
                     DRPDRLAATFTLQATNHQMIRD"
     gene            1834..2880
                     /locus_tag="VK103_01250"
     CDS             1834..2880
                     /locus_tag="VK103_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLQ82133.1"
                     /translation="MTVAQAGKRRRGNGPTIHTIADAAGVSTATVSYVLNDRDLTDDR
                     IRITPATRLRVLQAVDRLGYRANSMARGMRRGTTEQVCLALSHLDSPWTQAMIAAVSE
                     RVHRIGMTCLLLIDQNWQEFLARRGADGAVIQGHALTDDDVDRLRTLGERGVPVVALD
                     CDVEPDGFDVVRQRWRPALEQAVTSLLGRHERLACLTRGGGEHPTDDRRYRVYADVLR
                     RAGREIDPTMVRGVEADRYRAYRVALDLLDRSDRPTAVFAINDLAAISTLWAAHRLGI
                     TVPDELEVIGVGNSPEGVLADPPLSTVGPESVFGVVADLLVARLTNRDGPSGRVHDSD
                     WSFYPRSTTREEQS"
     gene            2877..4436
                     /locus_tag="VK103_01255"
     CDS             2877..4436
                     /locus_tag="VK103_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLQ82134.1"
                     /translation="MTGEIFGAPMTRRTLVGLATTAGLGTALTGCRVQTTSDNGDAGG
                     AAESGKIKFPDPMGDLPTEDVTYRAMMSGGIKQPYFEAVWKAFQDAHSNITMQLDATD
                     WKRINEVIPLGVRNDTAPDMFQKPQNVPVQVMVNEGWVAPLDDIIPDFEEWKKNYPAG
                     AFIPGVHVFDGKTYSWTFTSSRSQYGQLMFYDSALMSKAELDPTTDRFTWDDFRAAAK
                     KITDQGDGKSYGLFIAGTNKLGSTIMDFAELAGLPGGEMDFSTGEYNFTQPLLRDAID
                     LMLSVKDDGSMFPGYISLTQNEVDARFPQGKAGITFSGAWDVANWERDAPDYEYGLAM
                     PPMPNSGEMHHTVFTEGTAAPLYAYADSKYPAITGELLRYMGSVEGQVMLIILTKGIF
                     ASEIEEANEQAKRSDLVSGKAKQAGDIMADLRRISPMPQQANEDVAEVILAQKKVQPD
                     FADVMQGVFSGQVSNIDKALQDLQDRMNKSLDDAIEAARKKGADVSRDDWVFPNWDPD
                     TDYTLDMYESR"
     gene            4506..>4803
                     /locus_tag="VK103_01260"
     CDS             4506..>4803
                     /locus_tag="VK103_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLQ82135.1"
                     /translation="MLPAGLLALMFTFYPMVMSWWFSTLDWTGFTSHGAFIGLDNYRE
                     LVQDTMFWQAFGRSLIFMLVGTPLQVLVALLAAVVLNNESLKLAPVFRTMIFLPV"
BASE COUNT          845 a         1667 c         1591 g          700 t
ORIGIN      
        1 cagtggttct ggtacgaggg caacttcgcc gactacgaga ccaacaagct cgatcggctc
       61 ggcccggagg cggcgcggcc gcaccgcgcc acccaccgcc ggctcacccg cgactgaccc
      121 gccgtggccc gggtcacatc ccgacgacga tggcgtgctc cccggtggcc acggcagccg
      181 ggtcgccacc caggaccgtg accgccgagc cgccaccgag cagcggggcg accaggaccg
      241 cccgggcgac ggccaacgga tcgagtggtg cccgaccgtc gaccagcacc cggtcggcgg
      301 tcggtgccac gtcgagcacc tcggccagcg cacggccctc ccacaccggg tcgtccgggg
      361 tcggctcgct cccgacgtag tcgtcgtcct cgcccttcac cacctcggcc cagtcggtca
      421 catcgtccgg caacggcgaa gcgaatccga gccccagcgg gtgcagcgaa catgcgagcc
      481 gctgggcggc gttgtctccg aactccaggt ccggaccggt caccacggcc accgtgtccg
      541 ggtccggtgc cagcaccacc tccaccccga ccgtccagca cgctgcgacc cacaccagcg
      601 tcatccagtg cgccgggtgc cggaccgcca ccggcaacgc gatccggtcc ccggtgtcgg
      661 cgtcgagggt ccaggcgagc agattggccg acttggcgac ccagttgccg tacgtcgtcg
      721 ccgacaactc ggtccgcgca ccgtcggcgt cgtagcaggt gagcaacggt cgtgcgccat
      781 cccgtcggat gcggcgctgc aacaggtcca ggaaggtcac ggccccagcg taggcggcac
      841 ggcggcccga ccgacgaggt gtgtgatgat gacgccatgc agcttcggct cccggggcgg
      901 acgcaccagc aatggttcgc cgacaacgcc gccaactggg acgaccgagc acgggtgcac
      961 gccacctcgt ccggctacgg cctggacgac tacgtcaccg atccggacaa gctcagccac
     1021 accgtccgct acgacagtgc ggacctgggt gacctgaccg gggtacgggg cgtgcacctg
     1081 cagtgccacc tgggcaccga caccatctcg ctggcccgac tcggcgccac catgaccggg
     1141 gtggacctca gcccggaatc cgtggcgatc gccaaccggc tggccgcacg gaccggggac
     1201 gcggccgact tcgtcgccgc gaacgtgtac gacgcaccgg cggccctcgc ggcggccggg
     1261 cacccgcccg gcagcttcga cctggtctac accggtgggg gagcgctgtg ctggttgccc
     1321 gacatcgccg cgtgggcacg ggtctgcgcc gagctgctcg ccccgggcgg gcggctgcac
     1381 gtccgggact tccacccgat gatgctggcc ctggccgacg aacggcccga cgggttgctg
     1441 gtcgtggagc atccgtactt cgagaccgcc gaaccgcaga cctgggacga cggggcgacc
     1501 tacgtggccg gaggcgacga tgcccccgac atcaccgcga cggtcagcca cgagtggaac
     1561 cacgggctcg ccgagaccat cacggcggtg ttggacgccg gcctgcagct caccctggtc
     1621 cgggaggacc ggaccagcgg gtgggcgcgg ttccccaccg cgatggtcga cgcgcccggg
     1681 ctggagcacg agtacgagct cgccgaccgg ccggatcgac tggccgcgac cttcacgctc
     1741 caggcgacca accaccagat gatccgggac tgacccttga cagcaccgcg tgcgggccgt
     1801 tcactggatt aaaggtttaa ctgacacgat ggagtgaccg tggcccaggc tggcaagcgg
     1861 cgccgtggca acggcccgac gatccacacg atcgccgatg ccgccggtgt gtcgaccgcc
     1921 accgtgtcgt acgtgctcaa cgatcgcgac ctcaccgatg accggatccg gatcaccccg
     1981 gcaacccggt tgcgcgtgct gcaggccgtc gaccgcctcg ggtaccgcgc caactcgatg
     2041 gcccgcggga tgcggcgggg caccactgag caggtctgtc tcgccctcag ccacctcgac
     2101 tcgccgtgga cccaggcgat gatcgccgcc gtcagtgagc gtgtgcaccg catcgggatg
     2161 acctgcctgc tactgatcga ccagaactgg caggagttcc tcgcccgacg cggcgccgac
     2221 ggcgcggtga ttcagggcca tgcgctcacc gacgacgacg tcgaccgact gcgcaccctc
     2281 ggcgagcgcg gcgtcccggt cgtcgccctg gactgtgacg tggagcccga cggattcgac
     2341 gtggtccggc agcggtggcg gcccgctctc gaacaggccg tcacctcgct gctcggacgg
     2401 catgaacggc tggcatgcct cacccgtggc ggtggggagc acccgacgga cgaccgtcgg
     2461 taccgggtct acgccgacgt actgcgccgc gccggccgcg agattgaccc cacgatggtg
     2521 cgtggcgtcg aggcggaccg ctaccgggcc taccgcgtcg cactcgacct gctcgaccgc
     2581 tcggaccgtc cgaccgcggt cttcgcgatc aacgacctcg ccgcgatcag cacactgtgg
     2641 gccgcccacc ggctcggcat caccgttccc gacgagctgg aggtgatcgg ggtcggcaac
     2701 tcaccggagg gcgtgctggc cgacccaccc ctgtcgacgg tcggcccgga gtcggtcttc
     2761 ggcgtggtcg ccgacctgct cgttgcgcgg ctgaccaacc gggacggacc gagcggcagg
     2821 gtgcacgaca gcgactggtc cttctatcca cgaagcacca ccagagagga acaatcatga
     2881 ccggggagat cttcggagca ccgatgacga ggcggaccct cgtcggcctc gcaacgaccg
     2941 caggactcgg taccgcactc accggttgcc gggtccagac gacatccgac aacggcgacg
     3001 ccggaggcgc tgccgagtcc ggcaagatca agttccccga cccgatgggg gatctgccga
     3061 ccgaggacgt gacctatcga gcgatgatga gcggcgggat caagcagccg tacttcgaag
     3121 cggtgtggaa ggcgttccag gacgcccact ccaacatcac catgcagctg gacgccaccg
     3181 actggaagcg catcaacgag gtcatcccgc tcggggtccg caacgacacc gcaccggaca
     3241 tgttccagaa gccacagaac gtccccgtcc aggtgatggt caatgagggc tgggtcgccc
     3301 cactggacga catcatcccg gacttcgagg agtggaagaa gaactacccg gccggtgcgt
     3361 tcatccccgg tgtccacgtg ttcgatggca agacctactc gtggacattc acctcgtcac
     3421 gttcgcagta cggacagctg atgttctacg acagtgccct gatgtcgaag gcggagctgg
     3481 acccgaccac ggatcgcttc acctgggacg acttccgggc ggcggcgaag aagatcaccg
     3541 accagggtga cggcaagagc tatggcttgt tcattgccgg cacgaacaag ctcggcagca
     3601 ccatcatgga ctttgccgag ctggccgggt tgccaggtgg agagatggac ttctcgaccg
     3661 gcgagtacaa cttcacccaa ccgctgctgc gggacgcgat cgacctgatg ctgtcggtca
     3721 aggacgacgg gtcgatgttc cccggataca tctcgctgac ccagaacgag gtggacgctc
     3781 gcttcccgca gggcaaggcc gggatcacct tctcgggcgc gtgggacgtc gccaactggg
     3841 agcgcgatgc acccgactac gagtatggcc tggcaatgcc gccgatgccg aactcgggcg
     3901 agatgcacca cacggtgttc accgagggga cggcggcacc gctgtacgcc tatgccgaca
     3961 gcaagtaccc ggcgatcacc ggggaactgt tgcgctacat gggcagcgtc gagggccagg
     4021 tgatgttgat catcctcacc aagggcatct tcgcgtccga gatcgaggag gccaacgagc
     4081 aggccaagcg cagcgacctc gtgagcggca aggccaagca ggccggcgac atcatggccg
     4141 acctgcggcg gatcagcccg atgccgcagc aggcgaacga ggatgtggcc gaagtgatcc
     4201 tggcccagaa gaaggtgcaa ccggacttcg ccgatgtgat gcagggggtc ttcagcggac
     4261 aggtgagcaa catcgacaag gcattgcagg acctgcagga ccggatgaac aagagcctcg
     4321 acgacgcgat cgaggcagcc cgcaagaagg gtgccgacgt ctcacgggac gactgggtgt
     4381 ttcccaactg ggatccggac accgactaca ccctggacat gtacgagagc cgctgatcaa
     4441 tggcacgagc gacaccgacg cgtcactggc agcgggtcgt caccaatcgg tggtgctacc
     4501 tgttcatgct cccggcaggc ctcctcgcgc tgatgttcac cttctatccg atggtgatgt
     4561 cgtggtggtt ctcgaccctc gactggacag gcttcacctc ccacggggcc ttcatcgggc
     4621 tggacaacta ccgcgagctg gtgcaggaca cgatgttctg gcaggcattc gggcgatcct
     4681 tgatcttcat gctcgtcggc accccgttgc aggtgttggt ggccctgttg gccgcggtgg
     4741 tgttgaacaa cgagagcctg aagctcgctc cggtgttccg caccatgatc ttccttccgg
     4801 tgg
//
LOCUS       DATGUK010000081         3612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_551037,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000081 DATGUK010000000
VERSION     DATGUK010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551037"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VK103_01265"
     CDS             <1..305
                     /locus_tag="VK103_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017582708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLQ82136.1"
                     /translation="ELDHEVVVLPRDLPTEPLTADTWTSVGPGMVFVTVPDAKQVKNR
                     LHIDLAPHLSDDREAEIQRLLDLGATRADVGQGDAGWTVLADPEGNEFCVLSARER"
     gene            complement(332..1630)
                     /locus_tag="VK103_01270"
     CDS             complement(332..1630)
                     /locus_tag="VK103_01270"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HLQ82137.1"
                     /translation="MPESAPVRIVHLGLGAFFRAHQAWYTQLANELPGAEPWGIHALT
                     GRRPDAAELLNAQDCRYTLIERGPDGDQGIDIRSIVQAGPGADPDSYRPAISDPAVAV
                     ITSTITEKGYLVGADDAERIRAGEVPGSAIGRLVDGLRVRREQDGGPLAMVPCDNLTA
                     NGEHTERIVLALAEQVDPGLAGWISDHVSFVSTMVDRITPATTDADIADARTLVGWDD
                     RAPVVAEPFSEWVIAGTFPAGRPAWDRVGAQVVEDVEPYEQRKLWLLNGGHSTLAYVG
                     LSRGHATIAEAMADPVCVEALEQVWTDAAPCLPFDSEAIDEATRALTDRFTNARIKHR
                     LEQIAGDGSQKLPVRLVSVQRARIAQGIGIGAAFPVAIGAWVTHLATDKVNDPQAAPL
                     RERLAGATDATERARIALSFLDSELAEDSGLVAEIAAHVR"
     gene            complement(1623..2471)
                     /locus_tag="VK103_01275"
     CDS             complement(1623..2471)
                     /locus_tag="VK103_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HLQ82138.1"
                     /translation="MKFGYFTAATPDWTPQEVVTNLAAQGWDGVEWRLVDDPGADEVG
                     FWAGNRATWPLTGLEDNIAAMRDLPAQAGLEITGVGAYAACDDHDNVERVLAATAAMG
                     ASQVRVRVPGVGRDEHYSTVFDSCREHYRWVAERAAAHGVKALLELHHNTICASASSA
                     MRLFDGLDPAHVGVIHDMGNLLYEGWERPLWGAQMLGPYLAHVHVKNTVFESTGVGDD
                     GTVGYRGKAAALRRGQADLRTYLAELVQAGYDGWVVCEDFSTDLPLAQRTADNLAYLK
                     ECAPHA"
     gene            2675..>3612
                     /locus_tag="VK103_01280"
     CDS             2675..>3612
                     /locus_tag="VK103_01280"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HLQ82139.1"
                     /translation="MPADPTARGIARELYRAVADAPIISPHGHVNPSLLLDNEPFGDP
                     AELFITPDHYITRVLFSGAHIDGDRVGRTKDGVPSSAEPREIWRRLAENWHRFHGTPM
                     RYWMEHELAELFDVDQALSAETADAIYDQVATRLSQDSFRPRALFDRFAIEVLATTDD
                     PADDLAAHAALAADDSFSGRVLPTWRADRFMDPSSARWLPALDDLENAIDGADCRSYT
                     GLLEALRQSRQRFIAHGCTATDSGVPDAGSTPLPAAEAERIHAAGLRGEATEAEAIAY
                     RHNLLHRFAEMSCEDGLVMQLHPGVIRNHHAASFET"
BASE COUNT          527 a         1298 c         1266 g          521 t
ORIGIN      
        1 tggagctcga ccacgaggtc gtcgtgctgc cgagggacct gccgaccgag ccgctcaccg
       61 cggacacctg gacctcggtg gggcccggca tggtgttcgt gacggtcccg gacgccaagc
      121 aggtcaagaa ccgactgcac atcgacctcg ccccgcacct cagcgacgac cgggaggccg
      181 agatccagcg tctgctcgat ctcggcgcga cccgtgccga tgtcggtcag ggcgatgccg
      241 gatggacggt gctcgccgac ccggagggca atgagttctg cgtgctgtcc gcccgggaac
      301 gctgacgctc acccgcgccg gcgggtgctg gtcagcgtac gtgtgctgcg atctcggcga
      361 ccagccccga gtcctcggcc agctcggagt cgaggaacga cagcgcgatg cgggcgcgtt
      421 cggtggcgtc cgtggcgccg gccaaccgct cccgcagcgg ggcggcctgc gggtcgttga
      481 ccttgtcggt ggccaggtgg gtcacccagg caccgatcgc gaccgggaag gccgcaccga
      541 tcccgatccc ctgcgcaatg cgggcacgct gcaccgacac cagccgcacc gggagcttct
      601 gtgagccgtc cccggcgatc tgctccagcc ggtgcttgat gcgcgcgttg gtgaaccggt
      661 cggtcagcgc gcgggtggcc tcgtcgatcg cctcggagtc gaacggcagg cacggtgcgg
      721 cgtcggtcca cacctgctcg agggcctcca cgcacaccgg gtcggccatc gcctcggcga
      781 tggtggcgtg cccgcgcgac agtccgacgt aggcgagggt ggagtgtccg ccgttgagca
      841 accagagctt gcgctgctcg tacggctcga cgtcctcgac gacctgggca ccgacccggt
      901 cccacgctgg tcgcccggcg gggaaggtgc cggcgatgac ccattcggag aacggctcgg
      961 ccaccaccgg ggcccggtcg tcccagccga ccaacgtgcg ggcgtcggcg atgtcggcat
     1021 cggtggtcgc cggggtgatc cggtccacca tggtcgacac gaagctcacg tggtcgctga
     1081 tccagccggc gagcccgggg tcgacctgct cggccaacgc cagcacgatc cgttcggtgt
     1141 gctcgccgtt ggcggtcagg ttgtcacagg gcaccatcgc cagcgggccg ccatcctgct
     1201 cacgccgcac gcgcagaccg tcgaccagtc ggccgatcgc cgaacccggc acctcgcccg
     1261 cccggatccg ctcggcatcg tcggcgccga cgaggtaacc cttctcggtg atggtggagg
     1321 tgatcaccgc gaccgcgggg tcggagatgg ccgggcggta gctgtctggg tcggcgccgg
     1381 gaccggcctg cacgatgctg cggatgtcga tgccctggtc gccgtcgggt ccccgctcaa
     1441 tgagcgtgta gcggcagtcc tgcgcgttca gcagctccgc agcgtccgga cgacgccccg
     1501 tgagggcgtg gatgccccac ggctcggcgc cgggcagctc attggcgagc tgggtgtacc
     1561 aggcctggtg ggcacggaag aacgcaccga gcccgagatg gacgatgcgg acgggcgcgg
     1621 actcaggcat gcggggcaca ctccttgagg taggcgaggt tgtcggcggt ccgctgggcc
     1681 agcgggaggt cggtggagaa gtcctcgcac accacccagc cgtcgtagcc ggcctggacg
     1741 agctcagcga ggtaggtgcg cagatcggcc tgcccgcggc gcagcgcagc ggccttgccg
     1801 cggtagccga cggtgccgtc gtcgccgaca ccggtggact cgaagacggt gttcttcacg
     1861 tgcacgtggg ccaggtacgg gccgagcatc tgcgcgcccc acagtgggcg ctcccagccc
     1921 tcgtagagca ggttgcccat gtcgtggatg accccgacat gcgccggatc cagtccgtcg
     1981 aagagccgca tcgccgagga ggcggaggca cagatggtgt tgtggtgcag ctcgagcagg
     2041 gccttcaccc cgtgggccgc ggcgcgctcg gcgacccacc ggtagtgctc gcggcaggag
     2101 tcgaacaccg tgctgtagtg ctcgtcgcgg ccgacgccgg ggacgcggac ccgcacctgg
     2161 ctggcgccca tcgccgcggt cgcagcgagg acccgttcaa cgttgtcgtg gtcgtcacag
     2221 gcggcgtacg caccgacgcc ggtgatctcg aggccggcct gcgccggcag gtcgcgcatc
     2281 gcggcgatgt tgtcctccag gccggtcagc ggccacgtcg cccggttgcc ggcccagaag
     2341 cccacctcgt cggcacccgg gtcgtcgacc agtcgccact cgacaccgtc ccagccctgg
     2401 gcagcgaggt tggtgaccac ctcctgaggc gtccagtccg gggtggcggc ggtgaaatag
     2461 ccgaacttca tggtcgtccc tccaactcgg cggacactcg gtgggtgcgt cctcgctcga
     2521 cactagtcga tcccccagcg gccgaggaat gcgctcgccc agcggtccac tagagtgtga
     2581 gaaaccgatc acccgcgcgt ggtcggagaa gatcggccga cgagcagaac agcgaggaac
     2641 catgaccagc ttcgtcccgc acccggatcg tctgttgcct gctgacccca cggctcgcgg
     2701 gatcgctcgg gagctctacc gggccgtcgc ggacgccccg atcatctccc cgcacggcca
     2761 cgtcaacccc tcgctgctgc tggacaacga accgttcggg gacccggccg agctgttcat
     2821 caccccggac cactacatca cccgggtgct gttctccggg gcgcacatcg acggcgaccg
     2881 ggtggggcgc acgaaggacg gggtgccctc gtcggccgag ccacgcgaga tctggcgccg
     2941 gctcgccgag aactggcacc ggttccacgg caccccgatg cgctactgga tggagcacga
     3001 gctcgccgag ctgttcgacg tcgaccaggc gctgtcggcc gagaccgccg acgcgatcta
     3061 cgaccaggtg gccacccggc tgtcacagga ctcgttccgt ccccgcgcac tgttcgaccg
     3121 gttcgccatc gaggtgctgg ccaccaccga cgacccggcc gacgacctgg ccgcccatgc
     3181 cgcgctggcc gccgacgact cgttctcggg gcgggtgctg ccgacctggc gtgctgaccg
     3241 cttcatggac ccgtcgtccg cccggtggct gcccgccctc gacgacctgg agaacgcgat
     3301 cgacggagcc gactgccgca gctacaccgg tctgctcgag gcgctgcgcc agagccggca
     3361 acggttcatc gcgcacggct gcaccgccac cgactccggc gtccccgacg ccggctccac
     3421 cccgttgccg gccgccgagg ccgaacggat ccatgccgcc gggctgcgcg gcgaggccac
     3481 cgaggccgag gcgatcgcgt accggcacaa cctgctgcac cgcttcgccg agatgtcgtg
     3541 cgaggacggc ctggtgatgc aactgcaccc cggcgtgatc cgcaaccacc acgccgcctc
     3601 gttcgagacg ta
//
LOCUS       DATGUK010000082         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1141941,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000082 DATGUK010000000
VERSION     DATGUK010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141941"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VK103_01285"
     CDS             <1..1072
                     /locus_tag="VK103_01285"
                     /inference="COORDINATES: protein motif:HMM:NF037019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82140.1"
                     /translation="TGPELEAWLPVTGGEVTLAADAGQPYVIHPQEVAVADPEWGEGT
                     GLQNPFFTPGDLRGWQTSGASSVVLSDRGHHEVHLGEDGAAAHIRQPITGLEPGGRYA
                     ASVLVQIGAEPGETRRTTLTVDTPDGSSTTHLDRSTLKNTQNSDPKKGTRFQRIWTYF
                     TAPDDGTDPELRIETVAGDAPVLLDHVRVAAGSRPDAPEGAIVAEDFENVPFGYGVFV
                     SCNIRSHLSEPHAPYTNIGWNGKELDDVLDGGYSLKTRATGATLQYRSLPSTIEFRPG
                     HRYRVEFDYQSRTEADWVTGVDEPESRDIQADRLPKTTGTERWRYEFTAPDEGDTWIG
                     MRSRIGDSTEVVIDNLVVYELD"
     gene            complement(1057..1914)
                     /locus_tag="VK103_01290"
     CDS             complement(1057..1914)
                     /locus_tag="VK103_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLQ82141.1"
                     /translation="MRFFAPDVQVRSLGLACLGVGVQRGHLLTVGPRSVTRHVVVVVR
                     AGQGWFRSRGGPRVDVVAPAVLWLLPGEEHHYGVDPETGWDEFFVCFDGPATSLYTTM
                     GFLPRRTSWSPLADIGPLEPIRTQLLSVARPDNPLLDVEAAVLVHELVLALRSAASAD
                     PQERAERDLLDELTRNAFLPLSVTEHSHRLGLSTEQLRSLVRRRTGRTPKDHLLTVRL
                     EHAQELLSATDLSMAEVGRIVGYPDPAYFSRLFTRRLGVSPSVFRSEARVRERTRAQA
                     RADPDQSSS"
     gene            2078..>2228
                     /locus_tag="VK103_01295"
     CDS             2078..>2228
                     /locus_tag="VK103_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019356062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLQ82142.1"
                     /translation="MNTPILDLDLAELRGRTSMKWTTYDADVLPAWVAEMDCRPAEPV
                     RRAVGD"
BASE COUNT          375 a          764 c          767 g          322 t
ORIGIN      
        1 caccggtccc gagctggagg cctggctgcc ggtcacgggc ggcgaggtca cgctggccgc
       61 cgacgccgga cagccgtacg tgatccatcc ccaggaggtt gccgtggccg accccgagtg
      121 gggcgagggc accggcctgc agaacccgtt cttcacaccc ggggacctgc ggggctggca
      181 gacctccggg gcatcgagcg tggtgctgtc cgaccgcgga caccacgagg tgcacctcgg
      241 ggaggacggt gcggcagcgc acatccggca accgatcacg ggactggagc cgggcggtcg
      301 gtacgcggcc tcggtcctgg tgcagatcgg ggcggagccc ggcgagaccc ggcggaccac
      361 gctcaccgtc gacactcctg acgggtcgtc gacgacccac cttgatcgct cgaccctgaa
      421 gaacacccag aacagcgatc cgaagaaggg cacccggttc cagcggatct ggacctactt
      481 cactgcgcct gacgatggca ccgatcccga actgcggatc gagaccgttg ccggcgacgc
      541 ccccgtactg ctggaccacg tacgagttgc tgctggcagc cgaccggacg caccggaggg
      601 tgcgatcgtc gctgaggact tcgagaacgt cccgttcggc tacggggtct tcgtgtcgtg
      661 caacatccgc tcgcacctga gcgagccgca cgctccgtac accaacattg ggtggaacgg
      721 caaggagttg gacgacgtcc tcgacggcgg ctactccctc aagactcgcg cgaccggggc
      781 caccctgcag taccgttccc ttccctcgac catcgagttc cggcccgggc accggtaccg
      841 ggtggagttc gactaccaga gcaggaccga ggcggactgg gtgaccggag tggacgagcc
      901 cgaatcccga gacatccaag cggaccgact cccgaagacc acgggcaccg agcgctggcg
      961 ttacgagttc accgctcctg acgagggcga cacctggatc ggaatgcggt cgcggatcgg
     1021 tgacagtacc gaggtcgtga tcgacaacct ggtcgtctac gagctggact gatcgggatc
     1081 ggcgcgagcc tgcgcccggg tgcgttcccg gacccgggcc tcgctccgga acaccgatgg
     1141 tgacacgccc agacgtctgg tgaacagccg gctgaagtag gccggatccg ggtagccgac
     1201 gatcctgccg acctccgcca tgctcaggtc tgtggctgag aggagctcct gggcgtgctc
     1261 cagccgtacc gtcagcaggt ggtccttcgg ggttcggccg gtccgtcggc gcaccaggct
     1321 gcgcagttgc tcggtgctga gtcccagtcg atggctgtgc tcggtcacgc tcaacggcag
     1381 aaaggcattg cgggtcagct cgtcgagcag gtcccgctcg gcccgctcct gagggtcggc
     1441 ggaggcggct gagcgcaatg ccagcaccag ttcatggacc aggacggccg cctccacgtc
     1501 cagcagggga ttgtcggggc gagccaccga cagcagctgg gtacggatcg gctccagcgg
     1561 gccgatgtcc gccagcggtg accaggacgt tcgccggggc aggaaaccca tcgtcgtgta
     1621 cagcgaggtc gcggggccgt cgaaacagac gaagaactcg tcccacccgg tctccggatc
     1681 cactccgtag tggtgctcct cgccgggcaa cagccacagc actgccgggg ccaccacgtc
     1741 caccctcggt ccaccacggc tgcggaacca gccttgtcct gcccgcacca cgaccacgac
     1801 gtgtcgggtc acggaacggg gccccaccgt caacaggtgg ccccgttgga cgccgacgcc
     1861 gaggcaggcg aggccgagtg aacgcacctg gacgtcgggt gcgaagaagc gcatccagcg
     1921 ttgagcatcc acgaggccat catgctattg gagcggggct gcgtcggagg cagcccgcat
     1981 ccagcggctc cggcacggtg tcgagcgcat gccggggccg ttccgcgtcc cggtggcctg
     2041 tccgggccag tgccgtgtgc cgtctaacct tggccacgtg aacaccccca tcctcgacct
     2101 cgatctcgcc gaactgcggg gccggaccag catgaagtgg accacctacg acgccgacgt
     2161 gctgccggcc tgggtggccg agatggactg tcgtcccgcc gagccggtgc gtcgggcggt
     2221 cggggacg
//
LOCUS       DATGUK010000083         3133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1260590,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000083 DATGUK010000000
VERSION     DATGUK010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260590"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VK103_01300"
     CDS             <1..1093
                     /locus_tag="VK103_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF/VirB11 family protein"
                     /protein_id="HLQ82143.1"
                     /translation="VDSAIFGYGRWQPLMDDPDVENIEIRGHDNVFLVYADRIEEAAP
                     VADSDEELIEQLTFIATYARTPKPFSPAHPEMTLNLEDRFRLHAIAFDVVDRPTIVIR
                     QHKFTGLSLPELAARGMMPSHVADFLAAAVRAHRSIVVSGNQGVGKTTFVRALAMAME
                     PGEAVGVIETDAELFLHKLPGRGRVVNFTAREGSGEGVGPDGRPLGQFTVQQHLSASL
                     RQNLSRIIVGEVRDVEAASMFQAMQTGAGSLSTIHAQHSQATVERLVTAAAIGGAMGQ
                     ADAYRQIAVNIHLIVHLAAVDERGRGGEYRRHVNEITEIHGFAERSDGTTSSMPAMAR
                     LYTAAEGQLHSELMSPDLRNDTVGRGWQP"
     gene            1090..2025
                     /locus_tag="VK103_01305"
     CDS             1090..2025
                     /locus_tag="VK103_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82144.1"
                     /translation="MIMGVVGASVGFGLAAAVLLLVLGLRRRPRWSRRPGALGTGGLG
                     AVWMRWTRRPPGAAGRRRDLWWLGSGVLAVLAFALSGLPVTLVAVPLVTILVPWLLSN
                     PNAAGIVRTAAIDQWVRSLRSLLLSGSDNTLEGALQSSLATAPEAIREPVQLLVARIN
                     ARWPTERALAMLAAELADPTADVVAAALVLAAKRRGTGLVEVLDGLAVAVADEVRARR
                     RIEGERASARAAARYLTMIFAAMAAGLALLNPAYLSPYATPLGQLVLLASVLGFLVAL
                     WVVRRITVVKDPPRLWPTVTEARTGAGMRAEVWDA"
     gene            2018..2923
                     /locus_tag="VK103_01310"
     CDS             2018..2923
                     /locus_tag="VK103_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82145.1"
                     /translation="MPSWLLAACLCGAAAGGALVALWLCLTPTTPDLAETLTRLHSRP
                     DSSVRAGVAEVAAPTWPERLGGWVQRRTATLPGLRTPYRDLDLLGMSTARFHAHKAFW
                     AVVGLAAPTLLMGWSALLGLTPGWGTPVVLGLALAVAGWCLPHLTVRRAAAATRQRFS
                     RTITAYIDLVVLERLSGATLATSIIEPATVAEAPLFVRIRQTVNRHQLERKPPWSALR
                     ELADSLELPDLRELADTMELSGLKGAPMADQLKARAGDIRNAWLHHDVEAAGAASQRQ
                     VAATGLLLLCFLVFVGAPALLRLTG"
BASE COUNT          425 a         1098 c         1143 g          467 t
ORIGIN      
        1 ggtcgactcg gcgatcttcg ggtacgggcg ctggcagccg ctgatggacg acccggacgt
       61 ggagaacatc gagatccgcg ggcacgacaa cgtgttcctg gtctacgccg accggatcga
      121 ggaggccgcc ccggtcgccg actccgacga ggagctcatc gagcagctca ccttcatcgc
      181 gacctatgcc cgtactccga agccgttctc cccggcgcac ccggagatga cgctcaacct
      241 ggaggaccgc ttccgactgc acgcgatcgc cttcgacgtg gtggaccgac ccaccatcgt
      301 catccggcag cacaagttca ccgggctgtc gctgccggag ctcgccgcgc ggggcatgat
      361 gccgagccac gtcgccgact tcctggccgc cgcggtgcgg gcccaccgct cgatcgtggt
      421 gtcgggcaac caaggcgtcg gcaagaccac cttcgtgcgg gccctggcga tggcgatgga
      481 gcccggcgag gcggtcgggg tgatcgagac cgatgccgag ctgttcctgc acaagctgcc
      541 cggacggggt cgggtggtga acttcacggc ccgggagggc agcggcgagg gcgtcggacc
      601 ggacggacgg ccgctgggac agttcaccgt gcagcagcac ctgtccgcct cgttgcggca
      661 gaacctgtcg cggatcatcg tcggcgaggt ccgggacgtg gaggccgcca gcatgttcca
      721 ggcgatgcag accggtgccg gctcgctgtc gacgatccac gcccagcact cccaggcgac
      781 cgtcgagcgc ctggtcaccg ctgcggcgat cggaggggcg atggggcagg ccgacgccta
      841 ccgccagatc gcggtcaaca tccacctgat cgtgcacctg gccgcggtcg acgaacgcgg
      901 ccggggcggc gagtaccggc gccacgtcaa tgagatcacc gagatccacg gattcgccga
      961 acgcagcgac ggcaccacca gctcaatgcc cgcgatggcc cggctgtaca ccgcggccga
     1021 ggggcagctg cacagcgagc tgatgtcgcc ggacctgcgc aacgacaccg tcgggcgggg
     1081 gtggcagcca tgatcatggg ggtggtcgga gccagtgtcg ggttcggcct ggcggccgca
     1141 gtgctgctgc tggtgctcgg actgcgtcgc cgtccgcggt ggtcccggcg tcccggtgcg
     1201 ctcggcaccg gcggtctcgg tgcggtctgg atgcggtgga cacgtcgccc acccggggcg
     1261 gcggggcgcc gtcgtgacct gtggtggctc ggcagcgggg tgctcgcggt actggcgttc
     1321 gcgctcagcg ggctgccggt gacgctggtc gcagtacccc tggtgacgat cctggtgccg
     1381 tggctgctgt ccaaccccaa cgcggccggg atcgtgcgca ccgccgcgat cgaccagtgg
     1441 gtgcgctcgt tgcggtcgct gctgctgtcg ggatccgaca acaccctgga gggagcactg
     1501 cagtcgtcgc tggccaccgc cccggaggcc atccgggagc cggtgcagct gctggtggcc
     1561 cggatcaacg cccgttggcc caccgaacgg gcgttggcga tgttggcggc cgagctggcc
     1621 gacccgaccg cggacgtggt cgcggcagcg ctggtgctcg ccgccaagcg gcgcggcacc
     1681 ggattggtgg aggtgctgga cggtctcgcg gtggcggtcg ccgatgaggt gcgggcacgc
     1741 cgcaggatcg agggggagcg ggcctccgca cgtgccgcgg ccaggtacct gacgatgatc
     1801 ttcgccgcga tggccgccgg tctcgcactg ctcaacccgg cctacctctc cccgtacgcc
     1861 accccactgg gccagctggt gctgctggcc tccgtgctcg gcttcctggt cgcactgtgg
     1921 gtggtgcggc ggatcaccgt ggtgaaggac ccgccgcggc tgtggcccac cgtgaccgag
     1981 gcccgaaccg gtgcggggat gcgtgccgag gtgtgggatg cctagttggt tgctcgccgc
     2041 ctgcctgtgc ggggccgcgg ccggtggggc actggtcgcg ctgtggctgt gcctcacccc
     2101 gaccaccccg gacctggccg agacactcac ccgtctgcac agtcgcccgg actcgtcggt
     2161 acgggccggg gtcgccgagg tcgccgcacc gacctggccg gagcggctcg gcgggtgggt
     2221 gcaacgccgt accgccaccc tgcccggact gcggaccccg taccgggacc tcgacctgtt
     2281 ggggatgagc accgcccgat tccacgccca caaggcattc tgggcagtgg tcggactcgc
     2341 cgcccccacc ctgctgatgg gctggtcggc actgctgggg ctgacacccg gttggggcac
     2401 cccggtggtg ctcggactgg cgctggccgt ggccggctgg tgcctgccac acctgacggt
     2461 ccgtcgtgcc gcggcggcga cacggcagcg gttctcccgc accatcaccg cctacatcga
     2521 cctggtggtg ctggaacggc tctccggcgc caccctggcg acctcgatca tcgagccggc
     2581 gacggtcgcc gaagcgccgt tgttcgtgcg gatccggcag acggtgaacc ggcaccagtt
     2641 ggaacgcaag ccaccgtggt cggcgttgcg ggagctggcc gactcgttgg agctgcccga
     2701 cctgcgggag ctcgccgaca ccatggagct gtccgggctg aagggggcgc cgatggccga
     2761 ccagttgaag gcccgggccg gtgacatccg caacgcctgg ctgcaccacg acgtggaggc
     2821 ggccggggcg gcgtcccagc ggcaggtcgc cgcgaccggg ttgttgttgc tgtgcttcct
     2881 cgtcttcgtc ggggccccgg cgctgctcag gctgacgggg tgatcgccac gacacagccg
     2941 tcccgaccca tcaccgatgc ccccgccgcc atccggggga tcggtgcgac aacgcccacg
     3001 cggacaacgg aaaggagacc ccatgtccgt cctcgaactc aacgcccggc tcagcgcctg
     3061 gtgccacgtc cggctggtcc gcccgctcgg gctcgaccgt ccccgtgacg aacggggcaa
     3121 caactcgatc gag
//
LOCUS       DATGUK010000084         4942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1490198,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000084 DATGUK010000000
VERSION     DATGUK010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490198"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1245)
                     /locus_tag="VK103_01315"
     CDS             complement(85..1245)
                     /locus_tag="VK103_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HLQ82146.1"
                     /translation="MVDLSLLTTTSGSVRAVNTARVLTAIREAGPISRSELIERSGLS
                     KATVSGIVSGLLERDAVRETGKLQQGRGRSRILLEFNSAAMTVIGVQIADDRCQFVRT
                     DLAGAILDRRDAAVDLSDVAKVPGALARHIRRIATRPGGTVLGVGVGVPGVVDPAGRH
                     VTVALSHDWHEVDLAEHLERAVGLPVTLANRAKVAALGQLAAHPAGAGGRRPGEQVGQ
                     DLVYLYLGSGVIAGIVIGGRLHFGHDGTAGDLGHVMVDPRGPVCACGTRGCLQVLVGS
                     GAIVADAGVADWDELECAVAAGDRDALRAVRRVGDRLGMPLGHLVAAVTPSVIAVGGP
                     TARLGTPLIDAIDRAMRRCAPTAGPVDIVPADEDAAPRGAAMLWMQRAGLLR"
     gene            complement(1294..2556)
                     /locus_tag="VK103_01320"
     CDS             complement(1294..2556)
                     /locus_tag="VK103_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HLQ82147.1"
                     /translation="MSTLTDIRVESATVEVVGHRLDHPLVLSNGVVTELPEITARVTV
                     SDGCSSAEGYGAANLSDAWAWPDPALTSGAKHEAMVDYARGLAGSIGDRVGGPAHPLE
                     LGLRLHDSFLAETAHRAPVLARAICASAFDAAIHDAAGQLTARSAFSFYDDDAQVPSA
                     DELFPTGAVEAVRTVLRAPHAQLPGWWLIAPGDDLPAAVDRASSCGMTQFKIKLAGSD
                     PAGDADRVAAIHDAVSSRGATVRLSVDTNEGSASPAAVLEFLDRLATIRPAAQDALGY
                     LEQPTPRDSLSRHSWHEVGQRTPVLVDEALSSMDDLVAAGETGWSGFAVKTCKGHSFA
                     LVVAAWAAQHDMVVSVQDLTNIGRSAIHSYLLAAHLPSINGIELNSPQYLPGANEPWL
                     PRLAGLFRPTEGAHRLDPSGVVGLGSRL"
     gene            complement(2553..3581)
                     /locus_tag="VK103_01325"
     CDS             complement(2553..3581)
                     /locus_tag="VK103_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HLQ82148.1"
                     /translation="MDKIVAVPGHGIRVVPGTLPALGATDVLIAPQHSYISAGTELSS
                     VRRTTAAEPGGEGHQLGYSQAGIVLEVGAEVGRIAVGDHVVAIGQGAFHASRTIVGQN
                     LVVPVPEQVDPADAAIAAMFCFAIEAVHKSRVQLGQKVVVFGAGMMGQLASRLYQLSG
                     ADVAVVDGLGFRRAKLPAGVTAVGPDDTGWDALAAWAEPYGVEHACIGFGGDATDSVQ
                     RLKPLMSTAPDGVPAGRIVFPGGAEVHLTMASAMGNIELLSSAKAGPGYRDPIYESGA
                     DYPGVYVPYPVRRNVETMIALLAEGRLSVSELITHRYPHTDAATAYRDLAERPGEILA
                     ALLSYEQA"
     gene            complement(3671..4654)
                     /locus_tag="VK103_01330"
     CDS             complement(3671..4654)
                     /locus_tag="VK103_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HLQ82149.1"
                     /translation="MRQATVSLVSGPSVELARPPTSEAGFGQIIDHWSERISRVLPDQ
                     PDLVVLPELFDRPKVSGEPAAQYLSGEDRDAFARDKGSRVRDALAEIAVANSTYITHP
                     SYRVADDGSVHNSLEFIGRDGTTAAIYDKIMPTLGELAGIPVTPGHQVLAAELDFGRV
                     SAAICFDLNFDQVRSAIAQVQPELIVFSSAYHGGLVQNHWALSCRSWFVGCVYPPNRS
                     TIINPTGHEVASTSTYDWLITRTINLDYVVVHLGYNREKLSRLKARYGREVLIEDPGL
                     VGMVLVSSLSEEVTARAMIDEFEIQDLDTYFAESAAHLEPGGITVRREGGS"
BASE COUNT          780 a         1771 c         1649 g          742 t
ORIGIN      
        1 acggccgctg ttgccctcgg gctgtcggtc cccggtgtgc tgtcgctgcc gttcgagccc
       61 cggctcggct gaggtcggtt cccgtcagcg cagcaggccc gcccgctgca tccacaacat
      121 tgccgcgcca cgtggcgccg catcctcgtc ggccggcacg atgtcgacag gtccggctgt
      181 cggggcacag cgccgcatcg cccggtcaat cgcatcgatc aagggcgtcc cgagccgtgc
      241 cgttggcccg ccgaccgcga tcaccgatgg tgtcaccgcg gccaccaagt gccccaacgg
      301 catcccgagg cggtcgccga cgcggcgcac ggctcggagc gcatcgcggt cgccggctgc
      361 cactgcacac tccagctcgt cccagtcggc cacgcccgca tcagccacga tcgccccact
      421 cccgacgagc acctgcaggc acccccgcgt gccgcaggca cacactggac cacgcggatc
      481 gaccatcaca tggcccaggt cacctgcggt gccgtcgtgt ccgaagtgca gacgcccccc
      541 gatcacgatg ccggcgatga ccccgctgcc gaggtacagg tacaccaggt cctgccccac
      601 ctgctcgccc ggacgacgtc cgcccgcccc tgccgggtgg gcggccagct ggccgagcgc
      661 agccaccttt gcacggttgg ccagtgtcac agggaggccg accgcgcgtt ccaggtgctc
      721 tgcgaggtcg acctcgtgcc agtcgtgcga gagcgcgacg gtgacgtgac gaccggcggg
      781 gtcgaccacg cccgggacgc cgaccccgac tccgagcacg gtgccgcccg gcctcgtggc
      841 aatccggcgg atgtgacggg cgagggcacc cgggaccttg gcgacgtcgg acagatccac
      901 ggccgcatcc cgtcgatcca ggatcgcccc ggccagatcg gtccggacga actggcaacg
      961 gtcgtcggcg atctgcacgc cgatcacggt catcgctgcg ctgttgaact ccagcaggat
     1021 ccggctccgg ccgcgtccct gctgcagctt gccggtctcg cgtacggcat cgcgttccaa
     1081 caggccggag acgatgccgg agaccgtggc cttgctcaac cccgatcgct cgatcagctc
     1141 ggaccgagag atgggccccg cctcccggat tgcggtcaac acccgtgcgg tgttcaccgc
     1201 ccggacgctc cctgacgtcg tggtgagcag cgacaggtcg accatggccg tcactatcgc
     1261 acaccggcca cggccgtcac ggcggcccgg cggtcacaac cggctcccca gtccgaccac
     1321 cccggacggg tcgaggcgat gggctccttc ggtgggccgg aacagcccag cgaggcgagg
     1381 aagccatggc tcgttggcgc cgggcaggta ctgcggcgag ttcagctcga tgccgttgat
     1441 gctcggcagg tgcgccgcca gcaggtagga gtggatggcc gacctgccga tgttggtcag
     1501 gtcctgcacc gagaccacca tgtcgtgctg ggccgcccag gcggcaacga caagggcgaa
     1561 actgtggccc ttgcaggtct tgaccgcgaa cccactccag ccggtctcgc cggcagccac
     1621 gaggtcgtcc atcgacgaca gtgcctcgtc gaccagtacc ggcgtccgtt gcccgacctc
     1681 gtgccaggaa tgccggctca gtgagtcccg tggcgtcggc tgctcgaggt accccaacgc
     1741 gtcctgggcc gccgggcgga tcgtggcaag ccggtcaagg aactccagga ccgctgccgg
     1801 cgacgcgctg ccctcgttgg tgtccaccga caaccggacc gttgcgccac gggaggagac
     1861 cgcatcgtgg atcgctgcga cccggtcggc gtcaccggcg ggatcggatc cggccaactt
     1921 gatcttgaac tgggtcatcc cgcaactgct cgcccgatcc acggcggcgg gcaggtcgtc
     1981 cccgggggcg atcaaccacc agcccggcag ttgcgcgtgt ggcgcgcgga ggaccgtgcg
     2041 gaccgcctcc accgcaccgg tcgggaacaa ctcatccgcc gacgggacct gcgcatcgtc
     2101 gtcgtagaag ctgaaggcgg atcgcgcggt cagctgcccg gccgcatcgt ggatcgctgc
     2161 gtcgaaggca ctggcacaga tcgcgcgcgc cagcaccggg gcacgatgcg ccgtctcggc
     2221 aaggaaggaa tcgtgcagcc gcaggccgag ttccagcggg tgggcagggc cgccgacccg
     2281 gtccccgatc gatccggcca ggccccgggc atagtcgacc atggcctcgt gcttggcgcc
     2341 gctggtgagc gcgggatcgg gccatgccca cgcgtcactg aggttcgccg ccccgtaacc
     2401 ctcagcactg gagcagccgt cgctcacggt cacccgggcc gtgatctccg gaagttcggt
     2461 gacgacgccg ttgctgagca ccagtggatg gtccagccgg tggccgacga cctcgacggt
     2521 ggccgactcc acgcggatgt cagtgagcgt actcatgcct gctcgtaact gagcagcgcg
     2581 gccaagatct cgccgggacg ttccgccaag tcacggtagg ccgtcgccgc atcggtgtgt
     2641 gggtatcgat gcgtgatcaa ctccgacacc gagagcctgc cctcggccaa cagtgcgatc
     2701 atcgtctcga cgttgcgccg caccgggtac gggacgtaca ccccggggta gtcggccccg
     2761 gactcgtaga tcgggtcacg gtacccgggc cccgccttgg ccgacgacag cagctcgatg
     2821 ttgcccatcg ccgaggccat cgtgaggtgc acctctgcgc ctccggggaa cacgatccgc
     2881 ccggccggta ccccgtcggg cgcggtcgac atcagcggct tgagccgttg caccgagtcg
     2941 gtggcgtcgc caccgaaccc gatgcaggcg tgctccaccc cgtacggctc cgcccacgcc
     3001 gccagcgcat cccagccggt gtcgtcaggg ccgacggccg tcacgcccgc gggcagcttg
     3061 gcccggcgga accccagtcc gtcgacaacg gcgacgtccg cgccggacaa ctggtacagc
     3121 cgggacgcca gctggcccat catgccggcc ccgaacacga cgaccttctg acccagctgc
     3181 acccgagact tgtggacggc ctcgatcgcg aagcagaaca tcgctgcgat cgccgcgtca
     3241 gccgggtcga cctgctccgg gaccggcacg acgaggttct gaccgacgat cgtgcgggag
     3301 gcgtggaaag ccccctggcc aatcgccacg acatggtcgc ccaccgcgat cctgcccacc
     3361 tcggcgccga cctcgagcac gatgccggcc tggctgtagc ccaactgatg gccctcaccg
     3421 cccggctcag ccgcagtggt gcgccggaca ctgctcaact ccgtgcccgc actgatgtag
     3481 gagtgttgcg gggcgatcag cacgtcggtg gccccgaggg cgggcagcgt gccggggacc
     3541 acccggatcc cgtggccggg aacggcgacg atcttgtcca tcagggactc ttcctcctgt
     3601 catcggatgg ctcggtgcca ccgcgtgaat gggccgggtc ccgtgttcac gaacacgttc
     3661 ccggggacgg tcaggacccg ccctctcgcc gtacggtgat cccacccggc tccaggtggg
     3721 ccgcgctctc ggcgaagtag gtgtcgaggt cctggatctc gaactcgtcg atcatggcgc
     3781 gggcggtgac ctcctcggac aggctggaca ccagcaccat gccgaccagc cccgggtcct
     3841 cgatcagcac ctcgcggccg tatctggcct tcaaccgtga cagcttctcg cggttgtagc
     3901 cgaggtgcac caccacgtag tcgaggttga tcgtccgggt gatcagccag tcgtaggtgc
     3961 tggtcgaggc cacctcgtga ccggtcgggt tgatgatcgt gctgcggttc ggcggataga
     4021 cgcaccccac gaaccaggat cgacagctga gcgcccagtg gttctgcacc agccccccat
     4081 ggtacgccga ggagaacacg atcagctccg gttgcacctg cgcgatcgcg gaccgcactt
     4141 ggtcgaagtt gaggtcgaag cagatcgccg cgctcacccg accgaagtcg agctccgcgg
     4201 ccagcacctg gtgccccggg gtcaccggga tcccggccaa ctcccccagg gtcggcatga
     4261 tcttgtcgta gatcgccgcc gtcgtcccgt cgcgtccgat gaactccagc gagttgtgca
     4321 ccgatccgtc gtcggcgacg cgatagctgg ggtgggtgat gtaggtggag ttggccaccg
     4381 caatctcggc gagcgcgtca cgcacccgac tccccttgtc ccgggcgaac gcatcacggt
     4441 cctccccact caggtactgg gcggccggct caccgctgac cttgggacgg tcgaacagtt
     4501 cgggcagcac gacgaggtcc ggttgatcgg gcagcacccg gctgatccgc tcggaccagt
     4561 ggtcgatgat ctgcccgaac ccggcctcgg aggtcggcgg ccgcgccagc tcgacagagg
     4621 ggcccgagac gagggagacg gtggcctgac gcatctgtgg agacgctcct ctgggaaaca
     4681 tttgggatca atttgaaaac aaatgcatgg taggacgcgt gcgcgacacc ggtcaaggtc
     4741 cgcgccaccc cggggcggcg cttgcgggtg atcgtgtggt gggtcacagt ggtcccgtcc
     4801 cctcagacca ctggaggtca cgtgtcggcg ccggtcaagg aacaagaacc gtcagcgggc
     4861 agcgagcagc cgcaggaact gaagcgggtg atcggcccca agctgttgct gctgttcatc
     4921 gtcggcgaca tcctgggcac cg
//
LOCUS       DATGUK010000085         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_802782,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000085 DATGUK010000000
VERSION     DATGUK010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802782"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VK103_01335"
     CDS             complement(<1..671)
                     /locus_tag="VK103_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLQ82150.1"
                     /translation="MSDHPQSGGRLTRHVAETVRHQIRSGYWAPRTRLPPDAELARHH
                     GVGTNTVRRALALLAAEGLVVRGKGSGTRVAVHDSMAGRRRLIGIFVPSRRRIFPDIV
                     TGADEGARAAGARIVVRTTEFNRDAELQAIDELLEMSPDGLVVVPTLEGLIDTDDYLH
                     RLGSLDLPVVIAERRGPGAAAAGVSVVATDVHHAGYLAVQHLHRIGRRRLGLMSSRGT
                     ATSGD"
     gene            complement(678..>2965)
                     /locus_tag="VK103_01340"
     CDS             complement(678..>2965)
                     /locus_tag="VK103_01340"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HLQ82151.1"
                     /translation="HGNLRQLVMGTDDAPASADQLRRMQDLLRDGMDAGAFGLSAGLT
                     YVPGMFATTDELVALCEVVAEYDGLYVPHHRNYGSDVLGGFGECFDIARRSGVRLHLA
                     HTHLSYAQNEGRLPDLLTLFDRAEADGVDFSFDAYPYVASMSTLHAQFPSWSQVGTLD
                     DQLAMFSDPVVRERLRRAVDVDGSDGHQGFPVDWDTIVIGGVPDAATDWRWATGHSVS
                     AIADRLGEAPADAAMDLLVATRFATSCVMFVGNEDHVRALMRHPRHTVGSDGIMVGAK
                     PHPRAFGTFPRFLGTYVRELGVLELPEAIRHVTSAPADRLRLSDRGRIDVGAVADLVV
                     FDPATIGSPATYQEPHRFATGIDRVYVAGVPVVVGGSATGARPGRSLRASPPGTDELE
                     TPALVVDLDRTEANVAATATAAHAAGKSYLPHAKSHRSVELGRLQLTNGADGLCLAKL
                     GEAEAFADALVTDHPDLTVFVAYPVVGSGKARRALALAEQINLTVGTDSVAAAESIGE
                     VFAAAGRRCRMFLGIDSGLAREGVAPADAAAVATRIADLPGVWLDGIYTHEGSVMGAT
                     DRDDLRARSAQVAELMVGAATAIRDAGVPITEVSVGCSGSVDHLVDLPGLTQVRPGIN
                     TFGDMGQVALGNTDLDRVSVRVHVTVVSHPEPGRACIDAGTKSIGADLLPAGAHTDRY
                     PGHGLLVDAPGWQVAKLSEEHGWLRWVGDGDPPPLPVGTRLQLVPNHVCVVFGSLRRW
                     TAVRDGRVVGRYDCLPPGSSE"
BASE COUNT          431 a         1051 c         1079 g          404 t
ORIGIN      
        1 aagtcgccgg aggtcgcggt gccgcgagaa ctcatcagtc ccaggcggcg cctgccgatc
       61 cggtgcaggt gttgcacggc caggtagccc gcatgatgga cgtcggtcgc caccaccgag
      121 acaccggcgg ctgcggcccc aggccctcgg cgttcagcga tcaccaccgg caggtcgaga
      181 ctaccgaggc ggtgcaggta gtcgtcggtg tcgatcagcc cttccagagt cggcaccacg
      241 acgagtccgt ccggcgacat ctcgagcagc tcgtcgatgg cctgcagctc ggcgtcccgg
      301 ttgaactcgg tggtccggac cacgatcctg gcacccgccg cccgtgcgcc ctcgtcggcg
      361 ccggtgacga tgtcggggaa gatccgtcgc cgactgggga cgaagatgcc gatcagccgg
      421 cgacgacctg ccatcgagtc atgcacggcc acccgggtcc cggaaccctt gccccggacc
      481 accagtcctt cggcggccag aagggcgagc gcgcgacgga cggtgttggt accgacgcca
      541 tgatgacgcg cgagctcagc gtccggtggc agcctggtgc gtggggccca gtacccactg
      601 cggatctggt ggcgcaccgt ttcggcgaca tgccgggtga gccgtccacc ggactgcggg
      661 tggtccgaca ccccggatca ctccgaggag ccgggcggca ggcagtcgta ccggcccacc
      721 acgcgcccgt cccggaccgc ggtccagcgg cgcagcgatc cgaacaccac gcacacgtgg
      781 ttcgggacca gctgcaaccg ggtgccgacc ggcagcggcg gcggatcgcc gtcaccgacc
      841 cagcgcagcc agccgtgctc ctcggacagc ttggcgacct gccatcccgg cgcatcgacc
      901 agcagcccgt ggccggggta ccggtcggta tgggcgccgg ccgggagcag gtcggctccg
      961 atggacttgg tcccggcgtc gatgcaggcc cgtcccggct cggggtggct gaccacggtg
     1021 acgtggaccc gtaccgagac ccggtccagg tcggtgttgc cgagcgcgac ctgccccatg
     1081 tcgccgaagg tgttgatgcc ggggcgcacc tgcgtcagcc cggggaggtc gaccaggtgg
     1141 tcgacggacc cggagcagcc gaccgacacc tcggtgatcg gcaccccggc gtcgcgaatg
     1201 gccgtcgcgg caccgaccat cagctcggcc acctgcgccg agcgggcccg caggtcgtcg
     1261 cggtcggtgg cgcccatcac gctgccctca tgggtgtaga tgccgtccag ccagaccccg
     1321 ggcaggtcgg cgatccgggt ggcgaccgcg gccgcgtcgg ccggagcgac gccttcccgg
     1381 gcgagtccgg agtcgatgcc gaggaacatc cggcagcgcc ggccggcagc ggcgaacacc
     1441 tcgccgatcg actcggcggc cgccaccgag tcggtgccca cggtgaggtt gatctgctcg
     1501 gcgagcgcca gcgcgcgacg ggccttgccg ctgccgacca cggggtaggc gacgaagacc
     1561 gtcaggtcgg ggtggtcggt gaccagggcg tcggcgaacg cctcggcctc cccgagcttg
     1621 gccaggcaca gcccgtcggc cccgttggtc aactgcagtc ggcccagctc gacgctgcgg
     1681 tgcgacttgg catgcggcag atagctcttg ccggcggcgt gggctgcggt cgcggtcgca
     1741 gcgacgttgg cctcggtgcg gtccaggtcc accaccaggg cgggcgtctc cagctcgtcg
     1801 gtgcccggcg gcgacgcccg cagactccga cccggccgcg ctccggtggc gctgccgccg
     1861 accaccaccg ggaccccggc cacgtacacc cggtcgatgc cggtcgcgaa tcggtgtggt
     1921 tcctggtagg tggccggtga cccgatggtg gccgggtcga acaccaccag gtcggcaacc
     1981 gcgccgacgt cgatccgtcc ccggtcggac agccgcagcc ggtcggcggg ggcgctggtg
     2041 acgtggcgga tcgcctccgg caactcgagc acgccgagct cgcgcacata cgtgccgagg
     2101 aaccggggga aggtgccgaa ggcccgtggg tgcggtttcg cgccgaccat gatgccgtcg
     2161 ctgccgacag tgtggcgggg gtggcgcatc agggcacgga catggtcctc gttgccgacg
     2221 aacatcacac acgacgtggc gaagcgggtg gcgaccagca gatccatcgc agcgtcggcc
     2281 ggggcctccc cgagtcggtc ggcgatcgcg gacaccgagt gcccggtggc ccaccgccag
     2341 tcggtggcag cgtcggggac accgccgatc acgatggtgt cccagtccac cgggaagccc
     2401 tggtggccgt ccgagccgtc cacgtcgacc gcccggcgca gccgttcccg caccaccgga
     2461 tcactgaaca tcgcgagctg gtcgtccagt gtgccgacct gggaccagct cgggaactgt
     2521 gcatgcaggg tcgacatgct cgccacgtac gggtaggcgt cgaagctgaa gtcgaccccg
     2581 tcggcctccg cccggtcgaa cagtgtgagc aggtcgggca gccggccctc gttctgggcg
     2641 tagctgagat gggtgtgggc caggtgcagc ctgactccgg accggcgggc gatgtcgaag
     2701 cactcgccga acccacccag cacgtcggag ccgtagttgc ggtggtgcgg gacgtagagg
     2761 ccgtcgtact cggcgaccac ctcgcacagc gcgaccagtt cgtcggtggt ggcgaacatc
     2821 cccggcacgt aggtgaggcc ggcactcagc ccgaatgccc cggcatccat cccgtcgcgc
     2881 agcaggtcct gcatccgacg cagctggtcg gcggaggccg gggcgtcgtc ggtgcccatc
     2941 acgagctggc gcaggttgcc gtgcg
//
LOCUS       DATGUK010000086         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1025669,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000086 DATGUK010000000
VERSION     DATGUK010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025669"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VK103_01345"
     CDS             <1..204
                     /locus_tag="VK103_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HLQ82152.1"
                     /translation="CEYGADVVVQHLACQQRADELAGSLQRRLGLDEIEVSEVGAVIG
                     AHVGPGMLAVTVAGRLAPRAAEL"
     gene            312..1283
                     /locus_tag="VK103_01350"
     CDS             312..1283
                     /locus_tag="VK103_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004988256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HLQ82153.1"
                     /translation="MEVPRRRTTQKADGQAARRRLELLAEQLAAEERLAAERDGDLEP
                     GTEGQPVDVARQEDERTVPQTGAAARHRASALSGRDRLAAQMHDRLPGRWQSLLGRGL
                     TPQHIVVVVGVLAVLGCVTVWWVISAAPSTVTPVANPVGSAATPAPDAASDAGSEESS
                     GESGAPSEVSPAPPAATPTQVVVDVSGKVREPGIVTLPPGSRVADALDSAGGPRPGAK
                     LDTLNLARVLVDGEQIVVGLPPADRAGSAAVGSSGGAPDASVAPVNLNAATQEQLESL
                     PGVGPVTASAILEWRTEHGRFSSVDELLEVKGIGDATLADLRDRVTV"
     gene            1389..>2243
                     /locus_tag="VK103_01355"
     CDS             1389..>2243
                     /locus_tag="VK103_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HLQ82154.1"
                     /translation="MGVPAVVAWIGAVVAVSVEPEASVAAGLSMLAVGGLAAYRRWVL
                     SAITALVLAAVCIVAGARVAAVANGPVAELAEQHAVAHLRLQVDNDPRKVEGGGTPLV
                     VFDAEITRVQARGTSAQVSAPVVVWAERAAARFRLGDTVTATAVLEPSDDTEHTAQLD
                     VHRARLTGDPRAWWWDAAGAMRRSVTAAVARQTPHVAALVPALVHGDDQALPADLKED
                     FADSGLTHLLAVSGTNLTIVIGFLLVLARPLGVRGRGQVVLGTLGTVGFVILARPEPS
                     VVRAAAMGL"
BASE COUNT          316 a          781 c          842 g          304 t
ORIGIN      
        1 tgtgagtacg gcgccgacgt cgtggtgcaa cacctcgcgt gccagcagcg ggccgacgaa
       61 ctcgctggca gcctgcagcg acggctcggc ctggacgaga tcgaagtcag cgaggtcggc
      121 gccgtgatcg gcgcacacgt cgggccggga atgttggcgg tgacggtcgc cgggcggctc
      181 gcaccgcgcg ccgcggagct ctgagcaccg gttcggggca ggcccctcca caggcccatc
      241 gtgtgctcgg gttgtccaca cgccggcggc tcttgaggtc cagggggcgc gacggaacct
      301 aggctccgat cgtggaagta ccgcgtaggc gaaccacgca gaaggccgac ggccaggccg
      361 cacgccgtcg cctggagctg ctcgccgaac agttggccgc ggaagagcgg ttggccgcag
      421 aacgcgacgg cgacctggaa ccgggcaccg aggggcagcc ggtcgatgtt gcgcggcagg
      481 aggacgagcg gaccgtcccg cagacggggg ccgcggcgcg gcaccgagcc tccgcgctgt
      541 cgggacgcga ccggttggcg gcacagatgc acgaccggct gcccgggcga tggcagtcgt
      601 tgctggggcg cggtctgaca ccgcagcaca tcgtggtggt ggtcggtgtg ctggcagtcc
      661 tcggctgcgt gaccgtctgg tgggtgatct cggccgcgcc gtcgaccgtg acaccggtgg
      721 cgaacccggt cggctcggcg gccaccccgg cacccgatgc tgccagcgat gctgggagcg
      781 aggagtcgtc tggggagtcg ggagcgccct cggaggtgtc tcccgcgcca cctgccgcaa
      841 ccccgaccca ggtggtggtc gatgtgtccg gcaaggtacg cgaacccggg atcgtcaccc
      901 tgccgccggg ctcgcgggta gcggacgccc tcgactcggc cggcggcccc cggccgggcg
      961 cgaagctgga cacgttgaac ctggcccggg tgctggtcga cggagagcag atcgtggtcg
     1021 gcctgccacc ggccgaccgg gccggttcgg ccgctgtggg gtcgtccggc ggggcgccgg
     1081 acgccagcgt cgcaccggtc aacctgaacg ccgccaccca ggagcagctg gagagcctgc
     1141 ccggagtcgg cccggtcacc gcgtcggcga tccttgaatg gcggacggag cacggccggt
     1201 tcagcagcgt cgacgaactg ctcgaagtca aaggcatcgg cgacgcgacc ctcgcggatc
     1261 tgcgcgaccg cgtcaccgtc tagccgacga accgctcaac ggtggcaaca gcgcgctcac
     1321 cgggtccggc cggggatgcc cgggaccacg ccgctgggcc ggccggcgga cagcacgcgg
     1381 acctgcggat gggcgtaccc gccgtggtgg cgtggatcgg cgccgtcgtg gcagtcagcg
     1441 tcgaaccgga agcgtcggtg gccgccggcc tgtcgatgct cgccgtcggc ggcctcgcag
     1501 cataccgccg atgggtgttg tcagccatca ccgcgctcgt tctcgccgca gtgtgcattg
     1561 tcgcgggcgc gcgggtcgcg gcggtcgcga atggtccggt cgccgagctc gccgaacagc
     1621 acgcggtggc acacctgcgg ctgcaggtgg acaatgaccc tcgcaaggtg gaaggcggtg
     1681 gcacgccgct ggtcgtcttc gatgccgaga taacccgggt tcaagcacgc ggtacctccg
     1741 cgcaggtcag tgcgccggtg gtggtctggg ccgagcgggc ggcagcgagg ttccgcctcg
     1801 gcgataccgt caccgctacc gccgtgctgg agccgtccga cgacaccgag cacaccgcac
     1861 agctcgatgt tcaccgcgca aggctcaccg gggacccacg cgcctggtgg tgggacgccg
     1921 ccggagcgat gcggcgctcg gtcaccgcag ccgtcgcccg gcagactcct cacgtcgccg
     1981 cgctcgtccc cgcactcgtc cacggcgacg accaggcact gcccgccgac ctgaaggagg
     2041 acttcgccga cagcgggctc acccacctgc tggcagtgtc cggcaccaac ctgacgatcg
     2101 tgatcggatt cctgctggtg ctcgctcggc cgctgggagt ccgtggccga gggcaggtcg
     2161 tgctcggaac cctcggcacc gtcgggttcg tgatcctggc acgccccgaa cccagcgtgg
     2221 tacgcgccgc cgcgatgggg ctg
//
LOCUS       DATGUK010000087         4459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1026026,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000087 DATGUK010000000
VERSION     DATGUK010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026026"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VK103_01360"
     CDS             complement(<1..409)
                     /locus_tag="VK103_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HLQ82155.1"
                     /translation="MNILDATVARLDSTPATLADLTGGRPALLVNVASKCGMTPQYEQ
                     LEALQRDRGDELTVVGFPCNQFGGQEPGSADEIAEFCRTTYGVSFPMTEKVEVNGDGR
                     HPIWAALTTVADADGEAGDVQWNFEKFVLRADGT"
     gene            555..1052
                     /locus_tag="VK103_01365"
     CDS             555..1052
                     /locus_tag="VK103_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82156.1"
                     /translation="MSTTTPTSSRIPIAPERPTSITVAVRLMIAGAVVAGLRALIPII
                     WSDQIRANAIEQAGAQAEALSPSQLDTVVASALGGAVVGALLSALLWVWMAWANHGGR
                     RWARIVGTVFFAFSAISFLYGFATPGLPVEYVVGAVSLIIGAAAMIALWRPSSSEYIA
                     QASKR"
     gene            1004..1504
                     /locus_tag="VK103_01370"
     CDS             1004..1504
                     /locus_tag="VK103_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82157.1"
                     /translation="MAPELLGVHRAGVEAMTAPAPRPLRLAVTLMLVAAVASLGQAVL
                     ALVMADRIRSLALLGAVRNSVTGPFLAASAAEFTIVAAVVTGLAGAGLWAWLAVSAGR
                     RRTWVRVAATVLAGLTVLVLVASFVIMIITVVHVAAAAATVAAVVATVLLHQRGVVPG
                     VDAGRA"
     gene            complement(1550..3088)
                     /locus_tag="VK103_01375"
     CDS             complement(1550..3088)
                     /locus_tag="VK103_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HLQ82158.1"
                     /translation="MNRPNVLVFMTDQQRGVTVDPDHRLKAVTPHLDRFAQRATSFTR
                     AHTVAPHCCPSRTSFFTGRYPSEHGVWNNVNVPNALSRGPRDGTHFWSEDFADAGYRL
                     GFSGKWHVSNSAGPATYGWDELVLTAGPRGTGTLDEQYVAARDQQLAGLRRADPSDID
                     GKRGPGEVLRPGWPAFRLYGTDEDPFGDREVVDHAVARTHDSGRTDGDDPWMLYVGTL
                     GPHDPYVPPQRFLDLYDPESLELPASFADPMTDKPALYRRTRDRFDQLTEDEHRAALH
                     HYLAFCSYQDELFGRLLTALEETGQAEDTIVVYLSDHGDYTGEHGLWTKGLPSFTPAY
                     HVPLLMHVPGTTDRPRTTDAPVSLVDLGPTLAELCNVAAPPMSGRSLVADLTGDGPDD
                     PRDLVFQSNGNEVYGIQRTLLTDRWKLVVNLFDEDELYDLATDPDEMVNLIARESGPR
                     TVGREPLATVPAEHREVLTALYRRLWRFSLDHADEVLNPYIFTALATVGPLDAVAEVN
                     AADR"
     gene            complement(3085..3897)
                     /locus_tag="VK103_01380"
     CDS             complement(3085..3897)
                     /locus_tag="VK103_01380"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HLQ82159.1"
                     /translation="MLAGHRETSSPHYDLDGTLECPEALRPRARRSELHRGERPQVTV
                     LDHIHHRVMVSCQAYGDEPLNEPEIMAAMARSVVRGGACAIRAEGVADLQAAREAVDV
                     PLVGLEKIGTDGVFITPTLDSALRVAGTGCDILALDGTRRPRPDGRDLASTVAGIRER
                     HPTLLIMADCATRDDAVAAVDAGVDIVGSTLAGYTEDRAATAGPDLEVLAEMCATVGA
                     PVVAEGRYHTPDDVTAAFAAGAHSVCVGSAITHPQRITERFVAAVARRTEQR"
     gene            3883..>4459
                     /locus_tag="VK103_01385"
     CDS             3883..>4459
                     /locus_tag="VK103_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HLQ82160.1"
                     /translation="MPGQQLLALDVGQSGTALAVDGVRQEALLPALRGAAPIAEQLAD
                     LVRHALDLVDGGPVTVAIGSTGADSLTLAAEMRPRLPGTVATVLVAHDSVTSYLGCRG
                     VEPGCVLAVGTGTVALAVGTAVARVDGWGSLVGDAGSGYWIGRAAIDAALRAEDGRGR
                     RTALRERVAERFGGLDSLGVRLQADPDRVRRI"
BASE COUNT          566 a         1523 c         1662 g          708 t
ORIGIN      
        1 ccgtgccgtc ggcacgcagc acgaacttct cgaagttcca ctgcacgtca ccggcctcac
       61 cgtcggcatc ggcgaccgtg gtcagcgcgg cccagatcgg gtgacggccg tccccgttca
      121 cctcgacctt ctcggtcatc gggaaggaga ccccgtaggt ggtgcgacag aactccgcga
      181 tctcgtcggc actgcccggc tcctgcccgc cgaactggtt gcacgggaag ccgaccacgg
      241 tcagctcgtc gccgcgatca cgctgcaggg cttcgagctg ctcgtactgc ggggtcatcc
      301 cgcacttgct ggcgacgttg accagcaggg cggggcggcc gccggtcagg tcggccaggg
      361 tggctggggt gctgtcgagg cgtgcgacgg tggcgtccag gatgttcatc ccgccagcct
      421 gccacgtcgc ccatcatcac cggtgatgac cgcatcacgg atcgggcaag gccgaggagg
      481 agcgcactgg cccgtgcgct cgtcacagtg gaaggcctgc cagaccaggc cacccgaccc
      541 gaaaggcagc tgagatgtcc acgacaaccc ccacctcgtc caggataccg atcgccccgg
      601 aacggcccac ctcgatcacc gtcgccgtca ggctgatgat cgccggtgcc gtcgtcgccg
      661 ggctgcgagc gctgatcccg atcatctgga gcgaccagat ccgtgccaac gccatcgaac
      721 aggcgggcgc ccaggccgag gccctctcgc cgtcacagct cgacaccgtc gtcgccagcg
      781 ccctcggtgg tgcggtcgtc ggagcgctcc tcagcgccct gctgtgggtg tggatggcgt
      841 gggccaacca cgggggacgc cgctgggccc gtatcgtcgg caccgtcttc ttcgccttct
      901 cggcgatctc cttcctgtac ggcttcgcca ctcccgggct cccggtggag tacgtggtcg
      961 gggcggtgtc gttgatcatc ggggccgccg ccatgatcgc cctgtggcgc ccgagctcct
     1021 cggagtacat cgcgcaggcg tcgaagcgat gaccgcaccc gcaccgcggc cgctccggct
     1081 ggccgtcacg ctgatgctgg tcgccgcagt cgcctcgctc ggccaagccg tgctggcgct
     1141 ggtgatggcg gacaggatcc ggtcactcgc cctcctcggt gcggtccgca actcggtgac
     1201 cgggccgttc ctggccgcct cggccgcgga gttcaccatc gtcgcggccg tggtgaccgg
     1261 gctcgccggg gccggactgt gggcgtggct ggcggtgtct gccggtcgtc gccgtacctg
     1321 ggtgcgggtc gcggccaccg tgctcgccgg gctcacggtg ctggtgctgg tcgcgtcgtt
     1381 cgtcatcatg atcatcaccg tggtgcacgt cgctgccgcg gcggcaacgg tcgcggcggt
     1441 ggtcgccacc gtgctgctgc accagcgcgg cgtggtcccc ggcgtcgacg cgggtcgtgc
     1501 ctgaccggtc cggtcactga accgggtgcg gtcgcggtgc tggtcccgtt caccgatccg
     1561 cggcattcac ctcggccacg gcgtccagcg ggccgacggt cgccagggca gtgaagatgt
     1621 aggggttcag cacctcgtcg gcgtggtcga ggctgaacct ccacagccgc cggtacagcg
     1681 cggtgagcac ctcgcggtgc tcggccggga ccgtggccag gggttcgcgg ccgacggtgc
     1741 gcgggccgga ctcgcgggcg atcaggttca ccatctcgtc ggggtccgtg gcgaggtcgt
     1801 acagctcgtc ctcgtcgaac aggttgacca ccagcttcca ccggtcggtc agcagggtcc
     1861 gctggatccc gtacacctcg ttgccgttgc tctggaacac caggtcacgc gggtcgtccg
     1921 gtccgtcccc ggtgaggtcg gccaccagcg atcggcccga catcggtggt gcggcgacat
     1981 tgcacagctc ggcgagggtg gggccgaggt ccaccaggct gaccggggcg tcggtggtgc
     2041 ggggccggtc ggtggtcccg ggcacgtgca tcagcagcgg cacgtggtag gcgggggtga
     2101 aggacggcag gcccttggtc cacaatccgt gctcgccggt gtagtcgccg tggtcggaca
     2161 ggtagaccac gatcgtgtcc tcggcctgcc cggtctcctc cagcgcggtg agcaggcgtc
     2221 cgaacagctc gtcctggtaa ctgcagaacg ccaggtagtg gtgcagtgcg gcccggtgct
     2281 cgtcctcggt gagttggtcg aaccggtccc gggtacggcg gtacagcgcc ggcttgtcgg
     2341 tcatcgggtc ggcgaagctg gccggcagct ccagcgactc cgggtcgtac aggtccagga
     2401 agcgctgcgg tgggacgtac gggtcgtgcg ggccgagggt gccgacgtag agcatccacg
     2461 gatcgtctcc gtcggtgcgg ccgctgtcgt gggtgcgcgc gacggcgtgg tcgacgacct
     2521 cccggtcgcc gaacgggtcc tcgtcggtgc cgtagagtcg aaaggccggc cacccggggc
     2581 gcagcacctc cccggggcca cgtttgccgt cgatgtcact ggggtcggcg cgacgcagcc
     2641 cggcgagctg ttggtcgcgg gcggcgacgt actgctcgtc gagggttccg gtgccacggg
     2701 gcccggcggt cagcacgagc tcgtcccacc cgtacgtggc ggggccggcg gagttgctga
     2761 cgtgccactt gccgctgaag ccgaggcggt aaccggcgtc cgcgaagtcc tcgctccaga
     2821 agtgggtgcc gtcacgggga ccgcggctga gggcgttggg gacgttcacg ttgttccaca
     2881 ccccgtgctc gctggggtac cgcccggtga agaagctggt gcgtgacggg cagcagtgcg
     2941 gcgcgacggt gtgcgcccgg gtgaacgagg tggcccgttg cgcgaaccgg tccaggtgtg
     3001 gtgtgaccgc cttcagccgg tggtcggggt cgacggtcac cccgcgctgc tggtcggtca
     3061 tgaacaccag gacattgggg cgattcatcg ttgctccgtc cttcgggcga cggcggccac
     3121 gaagcgctcc gtgatccgct gcggatgggt gatggcgcta cccacgcaca ccgagtgggc
     3181 accggcggcg aaggcggccg tgacgtcgtc gggcgtgtgg tagcggccct ccgcaaccac
     3241 cggggcccca acagtggcac acatctcagc cagcacctcc aggtccgggc cggcggtggc
     3301 ggcacggtcc tcggtgtagc cggcgagggt gctaccgacg atgtccaccc cggcatcgac
     3361 cgcggcgacc gcgtcgtcac gggtcgcgca gtcagccatg atcaacaacg tcgggtggcg
     3421 ctcccggatg cccgcgacgg tgctggcgag gtcgcgaccg tccgggcgtg ggcgccgagt
     3481 gccgtcgagg gcgaggatgt cgcagccggt cccggccacc cgcagtgcgc tgtccagcgt
     3541 cggggtgatg aacacgccgt cggtgccgat cttctccagg ccgacgagcg ggacgtcgac
     3601 cgcttcccgg gcggcctgca ggtcggcgac gccctcggcg cggatcgcgc aggctcctcc
     3661 ccgtaccacg gaccgtgcca tcgccgccat gatctccggc tcgttgaggg gctcgtcccc
     3721 gtacgcctgg caggacacca tgacccggtg gtggatgtgg tccaggacgg tcacttgtgg
     3781 cctctcaccg cgatgcagct cagatcgacg tgctcggggc cgcagtgcct ccggacactc
     3841 tagagttcca tccagatcgt agtgagggga ggacgtctca cgatgcccgg ccagcaactg
     3901 ctcgcactcg acgtcggcca gagcggcacg gcgctggccg tcgacggcgt acggcaggag
     3961 gccctgttgc cggccctgcg gggcgccgct ccgatcgccg agcaactggc cgacctggtc
     4021 cgtcacgccc tcgacctggt cgatggagga ccggtgaccg tggcgatcgg gagcaccggt
     4081 gcggactcgc tcacgctggc cgccgagatg cggccccggc tgccggggac ggtcgccacg
     4141 gtgttggtgg cccacgactc ggtgacgagc tacctgggct gccggggggt cgagcccggg
     4201 tgcgtgctgg cggtgggcac cggcaccgtg gcgctggcgg tcggcaccgc ggtggcacgg
     4261 gtcgacggct ggggctcgct ggtcggggac gcggggtccg gctactggat cggccgggcc
     4321 gcgatcgacg cggcgctgcg ggccgaggac ggccgcggcc gccggaccgc gctgcgggag
     4381 cgggtcgccg aacggttcgg tggcctcgac tcgctggggg tgcggctgca ggcggatccg
     4441 gaccgggtcc gccggatcg
//
LOCUS       DATGUK010000088         4811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1383117,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000088 DATGUK010000000
VERSION     DATGUK010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383117"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="VK103_01390"
     CDS             <1..1480
                     /locus_tag="VK103_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82161.1"
                     /translation="TTAMQGISWADGQLVDTDGAATWLLDGRPVLGWQLGEVSVDGRS
                     VPTDPVTADWQVDELEQVTTAGPLRISLRHSLDRTWAIRLSVVNTGPAVVRVDRLGLL
                     VTTGPEWVGWSTRAGAEGSVAALPCRPGDPLLGFGLVQGELRGAGPDLVVGPLELPAG
                     TQQVVRLRGEPYGSAREFARGRHDLLPSWGWAEAHDEVQVHHPDLAVVAGPTAAPAGP
                     GEVADPDATILTGGPGDTTRLVLAGPRGLVDIEVGFAPDLEVLVEWFVAAGERSWPTG
                     RQGTRIPSTAAALVVQRQLAAPSRGGRPVADWSEAVEGVCARLAQDPPAGSSVTFAVA
                     LLARQAMLTGDPELAERATAESARAGVGLALVAPEVLAARRLTGLAALVPTDTGQRLA
                     ADHGPGVQPTGARWGLGLRGGRLAPDDPVADGRLLALTRMQAVLHPPRPRRWACGPEE
                     LLDRQDRRVRHQLGQRVRSGPDPADDPVVAEALAWLVLAPAG"
     gene            1504..2220
                     /locus_tag="VK103_01395"
     CDS             1504..2220
                     /locus_tag="VK103_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLQ82162.1"
                     /translation="MNRIPRRSVSQALVAALLTDQLMSWQQERGVDAVTVLDVGGGTG
                     GLASHLADLGHTVTVIDPSADALASLERRAAESGVGSRLTGRQGDASDVVDLVGPAAA
                     DLVVCHRVLEVVDHPVDALAAMATCLRPGGLLSLLVPGRRAAVLGHALNGQFEAAGDA
                     LTDERRFDPDQIVTMLGQVGFEVTAQHGVGAIADLVPESAVESQTARDQLTALEQRTG
                     TDPAFRALAPQLHVAARLAP"
     gene            2233..3477
                     /gene="dinB"
                     /locus_tag="VK103_01400"
     CDS             2233..3477
                     /gene="dinB"
                     /locus_tag="VK103_01400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HLQ82163.1"
                     /translation="MDPTTTGLGDAADGTDRRVVLHVDMDAFFAAVELRRRPELRGRR
                     MMVGGTERGVVVSATYEARADGVRSGMPMSRARRLSPRAVVIPPSHGLYREVSRQVLG
                     ILDDYTDLVEVASIDEAFLDITGAIRRLGPPARIGAMLRARVAEEQGLTCTVGIAPNK
                     FVAKVASTSAKPDGLLVVPPDRVLSFLHPLPVERMWGVGPATAERLHRLGLRTIADIA
                     STPVDTLVATFGHHHGHHLAKLARGHDPRPVVREEQERSIGSEETFAEDLADPALVRR
                     ELLRMADRTAARLRRAGLVGRTVSIVVRLADFTTLTRSARLGAPTDVTGEIHDQAVAL
                     HARLLRTHGVAARARVRLVGVRMEDLQPRDEAHLQPTLDEPDRGWRDAEEAIDRAVAR
                     YGDGAVHRAALGERRGRDTGPR"
     gene            3564..4004
                     /locus_tag="VK103_01405"
     CDS             3564..4004
                     /locus_tag="VK103_01405"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HLQ82164.1"
                     /translation="MEVTVALSESERRLLDQIEKGLAAEDPKLVHSFRGVPRQRRLHP
                     RRATAAGLGFLAGIGLLIGGMMTWPGISVLGFVVMVVCAVIALQSWQPVPAEDDPTGD
                     DDAPHHDDDDAHHRPTGVGDDSQFGAGFMDRMEERWRRRRDEDF"
     gene            complement(4010..>4811)
                     /locus_tag="VK103_01410"
     CDS             complement(4010..>4811)
                     /locus_tag="VK103_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82165.1"
                     /translation="IGFLPGEKVGDRWEVTTHDLHAWPELYFEGLGWVRWEPTPGNGA
                     TPPPWTIARAAGERPSTQASSTATTEETEPTAGPTSEVTGPSEAPSAESDPVTGEETT
                     GWGVGVWLAVGLAVVVLVGLMALPGLVRERRRRRRQSPGGPPAVAVEGAWQEVRDTVL
                     DHRGTWPAGSPRVIGHQLAHGMGRDDAEDVGRLALLVERSRYSRGLDDVSDVAGVDRL
                     TDRVRTAVIGPASRGQRVRARLWPRSLWHGGTLREALRRPRRHGRRGL"
BASE COUNT          608 a         1753 c         1811 g          639 t
ORIGIN      
        1 gaccactgcc atgcaaggga tctcgtgggc ggacggacaa ctggtcgaca ccgacggtgc
       61 ggcgacctgg ctgctggacg gacgacccgt gctcggctgg cagctggggg aggtgtcggt
      121 cgacggacgg tcggtgccga ccgacccggt gaccgccgac tggcaggtcg acgaactgga
      181 gcaggtgacc accgcgggac cgctgcggat ctccctgcgc cactcgctgg accgcacctg
      241 ggcgatccgg ctgtcggtgg tgaacaccgg cccggccgtg gtccgcgtcg accgtctcgg
      301 gctgctggtg accaccggcc cggagtgggt cggctggagc acccgggccg gggcggaggg
      361 atcggtggcg gcactgccgt gccgccccgg ggacccgctg ctcgggttcg gcctggtgca
      421 gggagagttg cgcggcgccg gaccggacct ggtcgtcgga ccgctcgaac tgcccgccgg
      481 gacgcaacag gtggtgcgtc tgcgcggtga gccgtacggc tccgcccgcg agttcgcgcg
      541 cggccgccac gacctgctgc cctcgtgggg gtgggcagag gcccacgacg aggtccaggt
      601 gcaccatccc gacctggcag tggtggcggg ccccaccgca gcccccgccg gccccggtga
      661 ggtggcggac cccgatgcga ccatcctcac cggtgggccc ggtgacacca cccgtctggt
      721 gctggccggg ccgcgcggcc tggtcgacat cgaagtggga ttcgccccgg acctcgaggt
      781 gctggtggag tggttcgtcg ccgccgggga acggagttgg ccgaccggtc ggcaaggcac
      841 ccggatcccg tcgaccgccg ccgccctggt ggtgcaacgc cagctggccg caccgtcgcg
      901 gggtggacgg ccggtcgccg actggtccga agcggtggaa ggtgtgtgcg cccggctggc
      961 gcaggacccg cccgcggggt cctcggtgac cttcgctgtc gccctgctgg cccgacaggc
     1021 gatgctgacc ggcgacccgg aactggccga acgggcgacc gcggagagcg cccgggccgg
     1081 ggtggggctg gccctggtcg caccggaggt gttggccgcc cgccgcctca ccgggctggc
     1141 agccctggtg cccaccgaca ccgggcagcg gctggcagcc gaccacggcc ccggggtgca
     1201 gccgaccggg gcccggtggg ggctcggcct gcgcggcgga cgcctcgccc ccgacgatcc
     1261 ggtggcggac ggccggctcc tcgccctgac gcggatgcag gccgtgctgc acccgccgcg
     1321 accacgccgg tgggcgtgcg ggccggagga actgctggac cgacaggacc gtcgggtccg
     1381 acaccaactc gggcaacggg tgcggtccgg gcccgatccc gccgacgacc cggtggtcgc
     1441 cgaggcgctg gcgtggctgg tgctggcgcc cgccggatga tccgggccgg taggatcggc
     1501 ccggtgaacc gcatcccacg tcgctcggtg agccaggccc tggtcgccgc gctgctgacc
     1561 gaccagctga tgagctggca gcaggagcgt ggtgtcgacg ccgtcaccgt gctcgacgtc
     1621 ggcggcggca ccgggggact ggcgagccac ctcgcggatc tcggccacac cgtcaccgtg
     1681 atcgacccca gtgccgatgc gctcgcctcc ctggaacgac gtgccgccga gtccggggtg
     1741 ggatcccggc tcaccggccg gcagggcgac gcctccgacg tggtcgacct ggtcggcccg
     1801 gcggcggccg acctggtcgt ctgccaccgg gtgctggagg tcgtggacca tccggtcgac
     1861 gcactggcgg ccatggccac ctgcctgcgg cccggcggtc tgctcagcct gttggtgccg
     1921 ggacgccggg ccgccgtcct gggccatgcc ctgaacggcc agttcgaggc cgcgggcgat
     1981 gcgctcaccg acgaacggcg gttcgacccc gaccagatcg tgacaatgct gggacaggtc
     2041 gggttcgagg tgaccgcgca gcacggcgtc ggggcgatcg ccgacctggt gccggagagc
     2101 gcggtggagt cccagactgc tcgcgaccag ctgaccgcgc tggagcaacg gaccggcacg
     2161 gacccggcct tccgcgcgct cgccccgcaa ctgcacgttg ctgcccggct cgctccctga
     2221 ccgcgaggcg cgatggaccc gaccacgacc gggctcggcg atgcggccga cgggaccgac
     2281 cgtcgggtgg tgctgcacgt cgacatggac gccttcttcg ccgcggtgga gctgcgccgt
     2341 cgtcccgagc tgcgggggcg ccggatgatg gtcggtggca ccgagcgtgg cgtggtggtg
     2401 tcggcgacct acgaggcccg cgccgacggg gtgcggtccg ggatgccgat gagccgggcc
     2461 cgccggctca gtccccgggc ggtggtgatc ccgcccagcc acgggctgta ccgggaggtc
     2521 tctcgccagg tgctcggcat cctggacgac tacaccgacc tggtggaggt ggcctccatc
     2581 gacgaggcgt tcctggacat caccggcgcc attcggcggc tcggcccgcc cgcacggatc
     2641 ggggcgatgc tgcgggcgcg ggtcgccgag gaacagggcc tgacctgcac ggtcggcatc
     2701 gcgcccaaca agttcgtggc caaggtcgcc tccacctcgg ccaaaccgga cgggttgctc
     2761 gtggtgccac ccgaccgggt gctgtccttc ctgcacccgc tgccggtgga acggatgtgg
     2821 ggcgtgggtc cggcgaccgc cgagcggctg caccggctcg ggctgcgcac cattgccgac
     2881 atcgccagca ccccggtcga caccctcgtc gccacgttcg gtcaccacca tggccaccac
     2941 ctggccaagc tggcccgcgg ccacgaccca cggccggtgg tgcgcgagga gcaggagcgc
     3001 agcatcgggt cggaggagac cttcgccgag gacctggcgg accccgccct ggtgcggcgg
     3061 gagctgctgc ggatggccga tcgcaccgct gcccggctgc gacgtgccgg cctggtgggc
     3121 cgcacggtga gcatcgtggt ccggctggcg gacttcacca ccctgacccg gtcggcccgg
     3181 ctgggcgcac cgaccgatgt caccggggag atccacgacc aggcggtcgc cctgcacgca
     3241 cgactgctgc gcacccacgg cgtcgccgcc cgggcccggg ttcgactggt cggggtacgg
     3301 atggaggacc tgcaaccgcg ggacgaggcg catctgcaac ccacactcga cgagccggac
     3361 cgcggctggc gggacgccga ggaggcgatc gaccgggccg tcgcacgcta cggcgacggg
     3421 gcggtgcacc gcgccgcgct gggggagcgt aggggccgtg acaccgggcc acggtgacgg
     3481 cctgatcact gccggttccc ttgcgacccg gctgcgcata cacttgagac agcaacctga
     3541 gccgaagtca tttcggccga gccgtggagg tgacagtggc cctttcagag tctgagcggc
     3601 ggctgttgga ccagatcgag aagggcttgg cggccgagga ccccaagctg gtccattcct
     3661 tccgaggtgt gccgcgacag cgacgtctgc acccgcgccg cgcgaccgcg gccgggctcg
     3721 gtttcctcgc cggcatcggg ctgctgatcg gcggcatgat gacgtggccg ggcatcagtg
     3781 tgctcgggtt cgtggtgatg gtcgtgtgcg ccgtgatcgc tctgcagtcg tggcagcccg
     3841 tcccggcgga ggacgacccc accggtgacg acgacgcacc gcaccacgac gacgatgacg
     3901 cgcaccaccg gcccaccggc gtgggtgacg attcgcagtt cggcgccggg ttcatggacc
     3961 ggatggagga gcgctggcgt cgtcgtcgcg acgaggactt ctgagcggct cacaaccccc
     4021 gacgaccgtg gcggcggggc cgccgcagcg cctcccgcaa cgtgccgccg tgccacaacg
     4081 atcggggcca caaccgggcc cgtacccgct ggcccctgct cgccggcccg atcacggccg
     4141 tgcgcacccg atcggtcagt cggtccactc cggcgacgtc gctgacgtcg tcgagccccc
     4201 gcgagtatcg ggagcgctcc accaggagtg cgagtcgccc gacgtcctcg gcgtcgtcgc
     4261 ggcccatgcc gtgggcgagt tggtgcccga tgacccgcgg tgacccggcc ggccaggtcc
     4321 cgcggtggtc cagcaccgtg tcgcggacct cctgccaggc gccctccacc gccaccgcgg
     4381 gtggcccgcc gggcgactgc cgtcggcgcc gtcggcgttc ccgtaccaga ccgggcagcg
     4441 ccatcagccc gacgagcacg acgacggcca gcccgaccgc gagccacacc ccgacgcccc
     4501 acccggtcgt ctcctcgccg gtcaccgggt ccgactcggc cgagggggcc tcggacggac
     4561 cggtgacctc gctggtcggc ccggccgtgg gctcggtctc ctcggtggtg gcggtgctgc
     4621 tcgcctgcgt cgacggccgc tcgccggcgg cccgcgcgat cgtccatggt ggtggggtcg
     4681 caccgttgcc cggggtcggc tcccaccgga cccagccgag cccctcgaag tagagctccg
     4741 gccaggcgtg caggtcatgg gtggtgacct cccagcggtc cccgaccttc tcgccgggca
     4801 ggaaaccgat c
//
LOCUS       DATGUK010000089         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1385043,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000089 DATGUK010000000
VERSION     DATGUK010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385043"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="VK103_01415"
     CDS             complement(<1..1309)
                     /locus_tag="VK103_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82166.1"
                     /translation="MADPYGTWSSPVTPASLVEGVRTLDGVAVTGSTVWWTQGDPADA
                     GRVSLWRRDGDAPAREVTPDHYVRTSVHEYGGGAWAVADSADGPVVVHSSWPDHRLWV
                     GVGDAEPRPITPEDSPHRYADLRVHPDRDLLLAVREDHTDPDAQPRNTLVALRLSGDN
                     ADGGTVLRSGPDFCASPELSASGLLAWVEWDHPAMPWDRTRLCVAPLADDAAALGPVR
                     VVVDRADTSVQFPAWADPTTLVLASDAPGWWQLHAVRVPTDPAAAVPEPEPLWTGEHD
                     VCSPMWGLGMKPYVVTERGVLAAWYVDGRQRLGLVAVDGGGPTVLRQSIDAVSAIAVD
                     GGQVAMVVGHADAPSEVVRWQLDAPQQWTRVAASSQQEPDPAWTSVPESVWWDSDLGP
                     VQGWYYPPTNPDVTPPEDERPPMIIKSHGGPTSMSRPLYLAAVQ"
     gene            complement(1331..2083)
                     /locus_tag="VK103_01420"
     CDS             complement(1331..2083)
                     /locus_tag="VK103_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HLQ82167.1"
                     /translation="MTEAENDVTEHDLTDRIAENLAGVRGRIDAACVRAGRRPEEVRL
                     LPVSKTFGPDTVRAAHAAGVRLLGENKVQELQAKHRETHEELPDLEWAAIGHLQTNKA
                     KVVAQLAAEFHALDSVRLAAELDKRLRTADRTLRVLVQVNSSGEESKYGLDPAEVPAF
                     AEEIAGFDRLRVAGLMTLAVNSPDLRMVAENFEVMTALQERLRSAGAPGHWDELSMGM
                     SGDFELAIEYGATTVRVGTAIFGARPQPDVWW"
BASE COUNT          299 a          830 c          779 g          307 t
ORIGIN      
        1 actgcaccgc cgccaggtac agcggtcgcg acatcgaggt cgggccaccg tgggacttga
       61 tgatcatggg cgggcgctcg tcctcgggcg gcgtcacgtc cgggttggtg ggtgggtagt
      121 accacccctg caccggcccc aggtcgctgt cccaccacac cgactccggc accgaggtcc
      181 aggccgggtc gggctcctgc tgcgagctgg cggccacccg cgtccactgc tgcggcgcgt
      241 ccagctgcca ccgcaccacc tcggacggcg cgtcggcatg tccgacgacc atcgcgacct
      301 gcccgccgtc cacggcgatc gcggagaccg cgtcgatcga ctgccgcagc acggtcgggc
      361 caccgccgtc gaccgcgacc agcccgaggc gctggcgccc gtcgacgtac caggcggcga
      421 ggacaccgcg ctcggtgacc acgtacggtt tcatgcccag cccccacatc ggggagcaca
      481 cgtcgtgctc gccggtccac agcggctccg gttccgggac cgcggcggcg ggatcggtgg
      541 gcacccggac cgcgtgcagc tgccaccagc cgggggcgtc cgaggccagc accagcgtcg
      601 tcggatccgc ccacgccggg aactgcaccg aggtgtcggc ccggtcgacc accacccgca
      661 ccgggcccag agcggccgca tcgtcggcca ggggtgccac gcacagccgg gtgcggtccc
      721 acggcatcgc gggatggtcc cactccaccc aggccagcag gccggaggcc gacagctccg
      781 gggaggcaca gaagtcgggt ccggaccgga gcaccgtccc accgtcggcg ttgtcgccgg
      841 acagccgcag cgccaccagc gtgttgcggg gctgcgcgtc ggggtcggtg tggtcctccc
      901 ggacggccag cagcaggtcg cggtccgggt gcacccgcag gtcggcgtag cggtgcggtg
      961 agtcctccgg ggtgatcggt cgcggctccg cgtcaccgac accgacccac agccgatggt
     1021 ccggccagct ggagtgcacg accaccggcc cgtcggcgga gtcggcgacc gcccaggccc
     1081 caccgccgta ctcgtgcacg ctggtgcgca cgtagtggtc cggggtcacc tcccgggccg
     1141 gggcgtcgcc gtcgcggcgc cacaggctga ccctgcctgc gtcggccggg tcaccctggg
     1201 tccaccacac ggtggagccg gtgaccgcga cgccgtccag ggtgcgcacg ccctccacca
     1261 gggaggccgg ggtgacgggg gaggaccagg tgccgtacgg atctgccatg cggctgatcc
     1321 tacggtcggc tcaccaccac acgtcgggct gtggacgtgc accgaagatc gcggtgccga
     1381 cccggacggt ggtggccccg tactcgatgg ccagttcgaa gtcgccggac atccccatcg
     1441 acagctcgtc ccagtggccc ggtgccccgg cggaccgcaa ccgttcctgc agcgcggtca
     1501 tcacctcgaa gttctccgca accatccgca ggtccgggct gttcaccgcc aacgtcatca
     1561 gcccggccac ccggagccgg tcgaacccgg cgatctcctc ggcgaatgcc ggcacctcgg
     1621 ccgggtcgag accgtacttg gactcctcgc cggacgagtt gacctgcacc agcacccgga
     1681 gggtgcggtc ggcggtccgc agccgcttgt cgagctcagc ggccagccgg acgctgtcga
     1741 gggcatggaa ctccgcggcc agctgcgcga ccaccttggc cttgttggtc tgcaggtggc
     1801 cgatcgccgc ccactccagg tcgggcagct cctcgtgggt ctcgcggtgc ttggcctgca
     1861 actcctggac cttgttctcc cccagcagcc gcaccccggc cgcatgggcg gcccggacgg
     1921 tgtcggggcc gaacgtcttg gagaccggca gcagccgcac ctcctcgggg cggcgcccgg
     1981 cgcggacaca ggccgcgtcg atccggccac ggacaccggc caggttctcc gcgatgcggt
     2041 cagtgaggtc gtgctcggtc acgtcgttct cagcctccgt catggatcca gacgacggca
     2101 ttgttgccgc cggtgcaggt ggtgagcacg tcgttgcgga cgcactccat cgtgtagtcc
     2161 ttcttggtga ccgggctgtg cgcccggacg gagaactcgg tcgcgtcggg cccga
//
LOCUS       DATGUK010000090         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_809615,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000090 DATGUK010000000
VERSION     DATGUK010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809615"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VK103_01425"
     CDS             <1..783
                     /locus_tag="VK103_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLQ82168.1"
                     /translation="ESTGLRTGDLLDGPDGLVRMGPLKIISDGSLHTRTAYCCEPYAD
                     GASLPHPRGIQNVVPAELHDLLDRARRAGLSVALHAIGDAAVHDALDAFAATGVTGSI
                     EHAQLVTDTDLPRLAELGVVASVQPAHLVDDRDVIDQCWPDSGRRCFRTATMINSGVD
                     VRFGSDAPVSPLDPWLAMAAAVHRGDPADPAWHPQEALSVRQALACSTDGIAHLAVGG
                     PGDMVLLDDNPLLWQGSSAELASRLRAVEVAATVVAGRPVER"
     gene            complement(790..1380)
                     /locus_tag="VK103_01430"
     CDS             complement(790..1380)
                     /locus_tag="VK103_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017547080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HLQ82169.1"
                     /translation="MADLVADQQVMAIFRNLGTDRTVALARRAWDLGIDLVEVPVSEP
                     GAWETLEAVVVEGRTRDRPVGAGTVIAPEQVARCAELGVGFTVAPGLDHGVVEASAHA
                     GIPHLPGVSTPSEIQHALRLGCHAMKAFPASVLGTSWFTAMRGPFPGVDLVATGGITA
                     GNAREFLDAGAAMVALGSALADESQLDAVGALVTGR"
     gene            complement(1434..2474)
                     /locus_tag="VK103_01435"
     CDS             complement(1434..2474)
                     /locus_tag="VK103_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HLQ82170.1"
                     /translation="MDHHGPVVLCVGETMALLTPQHAPAGLWLDVHQAGAEGNVAAGL
                     AHLGCRSRWLSRLGDDEFAPVITDFLASRGVDVAQVVTDPERPTGLMTKSVSGTGTDQ
                     RRTRVRYARAGSAASGLSPDDLTAAALDRVTVCHLSGITPALSDSCADLVEAVVVERR
                     LGPGITVSFDVNHRPALWADGAAGPTLHRIAAAADVVLVGLDEARELWGCRTPGAVRD
                     LLPEPRRLVVKDGAVAAHAFVRSDDGTDHPVVQPALRAEVVEAVGAGDAFAAGYLAGL
                     VAPDPGVPAGGGGAELRALRLGHALAACTLQSMLDLPAWPSAEVLRSWTRLDETGWRQ
                     LHVTPADLEGRP"
     gene            2690..3289
                     /locus_tag="VK103_01440"
     CDS             2690..3289
                     /locus_tag="VK103_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HLQ82171.1"
                     /translation="MVAMSTTWEGVRPVTSDHVADDRSEDFARFVAQHDRRLLGFAHL
                     ITGNHADAEDILQTALAKAYLKWARLGRDDFNAVAYIRRIIINEHRSLWRRAFKRREY
                     ATDALPEQPVHDAPVEDETWALVQALPPRQRTVVALRFYSDLSVAETAELMHCSEGTV
                     KSQTSRALASLRRAVREQDPPPEDEPAPTEHGDREGGDR"
     gene            3286..>3482
                     /locus_tag="VK103_01445"
     CDS             3286..>3482
                     /locus_tag="VK103_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82172.1"
                     /translation="MSEHDDTHDLHHRLHRHADGMRPSSNLVAGSLGRARRIRRRRTI
                     VATMTAVVVAGGGIAGAGALRG"
BASE COUNT          532 a         1349 c         1165 g          436 t
ORIGIN      
        1 gagtcgacgg gactgcgcac cggtgacctg ctcgacgggc ccgacgggct ggtgaggatg
       61 ggaccgctca agatcatctc cgacgggtca ttgcacaccc gcaccgccta ctgctgcgag
      121 ccgtacgccg acggcgcgtc cctgccccac ccgcgcggga tccagaacgt cgtacccgca
      181 gagttgcacg atctgctgga ccgcgcccgc cgggccggtc tgtcggtcgc cctgcatgcg
      241 atcggcgacg ccgcggtgca cgatgccctc gacgcgttcg ccgcaaccgg cgtgaccgga
      301 tcgatcgaac atgcccaact cgtcaccgac accgacctgc cccggctggc cgagctcggc
      361 gtcgtcgcca gtgtgcaacc ggcccacttg gtcgacgacc gggacgtgat cgaccagtgc
      421 tggccggaca gcggccgccg ctgcttccgt accgccacca tgatcaactc cggggtggac
      481 gtccgtttcg gctccgacgc ccccgtctca cccctggacc cgtggctggc gatggcggcc
      541 gccgtgcacc gcggcgaccc ggccgacccg gcctggcacc cccaggaggc gctgtcggta
      601 cggcaggcac tcgcctgttc caccgatggc atcgcacacc ttgccgtcgg cgggcccggg
      661 gacatggtgc tgctcgacga caatcccctg ctgtggcagg gaagcagcgc cgaactggcc
      721 tcccggttgc gcgccgtgga ggtggccgcc acggtcgtcg ccgggcgtcc ggtcgagcgc
      781 tgagaccggt cagcggccgg tgaccagtgc gccgaccgca tccaactgcg actcgtcggc
      841 cagggccgag ccgagggcca ccatcgccgc cccggcgtcc aggaactcgc gggcgttgcc
      901 cgccgtgatc cctccggtgg caaccaggtc caccccgggg aacggccccc gcatcgcggt
      961 gaaccaggac gtgccgagga ccgacgccgg gaatgccttc atcgcatggc atcccagtcg
     1021 cagcgcgtgt tggatctccg acggtgtcga cacaccgggc agatgcggga tgccggcgtg
     1081 cgcgctggcc tccaccacac cgtggtcgag cccgggcgcg acggtgaagc cgacgccgag
     1141 ctcggcgcac cgcgcgacct gctccggcgc gatcacggtg ccggcaccga ccgggcggtc
     1201 ccgggtacga ccctccacca ccacggcctc caaggtctcc caggcccccg gttccgagac
     1261 gggcacctcc accaggtcga tgccgagatc ccacgcccgc cgggcgaggg cgacggttcg
     1321 gtcggttccc agattgcgaa agatcgccat cacctgctga tcggcgacca gatcggccat
     1381 ccgacgatgg atcggtgact cccgcaccgc attcgatgca tcgggccatt cggtcatggt
     1441 cgtccctcca ggtctgctgg tgtcacgtgc agctgtcgcc acccagtctc gtcgagccgt
     1501 gtccaggacc gcagcacctc ggccgatggc cacgccggca ggtccagcat cgactgcagg
     1561 gtgcaggcgg ccagcgcatg gcccagccgg agcgcgcgga gctccgcacc accgccaccg
     1621 gcaggcaccc ccggatccgg cgccaccagg cccgccaggt agcccgcggc aaaggcgtca
     1681 ccggccccga ccgcctccac cacctcggcg cgcagtgccg gctgcaccac cggatggtcg
     1741 gtcccgtcgt cggaccggac gaaggcgtgg gccgcgacag caccgtcctt gacgaccagg
     1801 cggcgcggct ccgggagcag gtcccgtacg gctccgggcg tccggcagcc ccacagctcc
     1861 cgggcctcgt cgagaccgac cagcaccacg tcggccgccg ccgcgatccg gtgcagcgtc
     1921 gggccggccg cgccgtcggc ccacagcgcg gggcgatggt tgacgtcgaa gctcaccgtg
     1981 atccccgggc cgagccgccg ctcgacgacc accgcttcga ccaggtcggc acaactgtcc
     2041 gacagcgccg gggtgatgcc ggacaggtgg cacaccgtca cccggtcgag ggccgccgcg
     2101 gtcagatcgt ccggggagag cccgctggcc gccgaaccgg cccgcgcgta ccgcacccgg
     2161 gtccgccgct ggtcggtccc cgttccgctc actgatttcg tcatcagccc ggtgggacgc
     2221 tccggatcgg tcaccacctg ggcgacgtca acgccccggc tcgccaggaa atcggtgatc
     2281 accggggcga actcgtcgtc ccccagacgc gacaaccacc ggctacgaca ccccaggtgg
     2341 gccagaccgg ccgccacatt gccctcggca cctgcctggt gcacgtccaa ccacagcccg
     2401 gcgggcgcgt gctgcggggt gagcagcgcc atcgtctcgc cgacgcacag caccacgggc
     2461 ccgtggtggt ccattgcttc cctccgccca gcctcccggc ccgggcacct gcccggaccc
     2521 tgcaccgtac ggcgtcccac cctgccctga ccggccgacc cggtccagtc gtgcgaccga
     2581 cgtccggacg catgcaaccc cgcggcggtc ctggtgcgtt cccccaccag gaccgcatga
     2641 cgcgacccgg accggcacaa cgacccgagc agcaggaggt ggatgcgaga tggtggcgat
     2701 gtcgaccacc tgggaagggg tccgtcccgt aacctcggac cacgtggctg acgatcggtc
     2761 ggaggacttc gcgcgcttcg tggcccagca cgaccgacgg ctgctggggt tcgcgcacct
     2821 gatcacgggc aaccatgccg acgccgagga catcctgcag acagcgctgg ccaaggccta
     2881 cctgaagtgg gcccggctcg gtcgcgacga cttcaacgcg gtcgcctaca tccgccggat
     2941 catcatcaac gaacaccgca gcctgtggcg ccgcgcgttc aagcgccgcg agtacgccac
     3001 cgacgccctc ccggaacagc cggtgcacga cgccccggtg gaggacgaga cctgggccct
     3061 ggtgcaggcg ttgccgccac gccagcggac cgtggtggca ctgcggttct actccgacct
     3121 cagcgtcgcc gagaccgctg agctgatgca ctgctccgag ggcacggtga agagccagac
     3181 gagccgagcc ctggccagcc tgcgccgtgc cgtacgagag caggacccgc caccggagga
     3241 cgagcctgca ccgaccgagc acggcgaccg ggaaggagga gaccgatgag cgagcacgac
     3301 gacacgcacg acctgcacca ccgcctgcac cgccacgccg acgggatgcg tccgagcagc
     3361 aacctggtgg ccggctcact gggccgcgcc cgccggatcc ggcgacgccg cacgatcgtc
     3421 gccaccatga ccgcagtcgt ggtggccggc ggcggcatcg ccggggccgg ggcactgcgt
     3481 gg
//
LOCUS       DATGUK010000091         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_451542,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000091 DATGUK010000000
VERSION     DATGUK010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451542"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..990)
                     /locus_tag="VK103_01450"
     CDS             complement(100..990)
                     /locus_tag="VK103_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82173.1"
                     /translation="MQHILDDLAAGRIDTEEAARRIAVLQSESGSVDATGGPGAEPSD
                     DELAGERPRSDDGFRGYAPPPPPKAFGDEQRRSGARASGAKGVERLDVRCSGRRVRIV
                     GDSGVSMVSIDGPHVLRRNGRVLEITSEGSLTPSFDGIGLLRSRSIKEARDNAFGGKE
                     LVIRVNPSLLVEVELSGGGLTTAGVAYLGKVRVTGGGATLEGVAECGDVLVQAGQALV
                     SGTFRSGRSRIRVESGRGVVELGDDSNVTVRGEAQLGRVAWTGAHTGGGDEVVLGHGN
                     ARLDVEAILAHITVRAGSAA"
     gene            complement(996..>2126)
                     /locus_tag="VK103_01455"
     CDS             complement(996..>2126)
                     /locus_tag="VK103_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLQ82174.1"
                     /translation="RGGAGRGRARALGGARGRRRTAATDEGHSGQRGQRGERASPAGR
                     RIGHEGVSEAGELLHSLCHSGPRAVMPGSGFARHRCRIVPSPRAQRWGSFPDRFGDMA
                     GFAIVHILIVGGAGVLGSAITPHLRSSGHRVTTLGPDPAGRTEDHVVADATDFEALAD
                     AFDGVDAVVNFALRTPRGPGADERTEPVRAGFAVNVGSVYTQLRCAARAQVPAFVQIS
                     TMAVLEGYGQRRRSALAETDNTTFYGLTKRMAETACEAEAARSPQLTVTTLRLAYPTR
                     EDWWPQWGNPVATQPTGVPSCADTEFAALHPQDLASAVELAAARRGPYLCTAVTADVG
                     EASLTDQWPHPLGWRPRHVLGDGPESTGDGRAGGGAGSIFVA"
BASE COUNT          302 a          810 c          719 g          295 t
ORIGIN      
        1 cctcagccag tgacaaggtc ccgtgccgat cggtacggga ccttgttgca tgtccactcc
       61 tcgacttcgc tcgaggtgcg gagggttcga ggtgcggggt caggccgccg agccggcccg
      121 caccgtgatg tgggcgagga tcgcctcgac gtcgaggcgg gcgttgccgt gaccgagcac
      181 gacctcgtcg ccgccgccgg tgtgggcgcc ggtccaggcc acccggccga gctgcgcctc
      241 gccgcgcacc gtgacgttgg agtcgtcacc cagctcgacc acgccgcgcc ccgactccac
      301 ccggatccgg gaccgcccac tgcggaaggt gcccgacacc agggcctgcc ccgcctgtac
      361 gagcacgtcg ccgcactcgg ccaccccctc cagggtggcg ccgcccccgg tcacccggac
      421 cttgcccagg taggcgaccc cggcggtggt gagcccgcca ccggacaact cgacctcgac
      481 gagcagtgac gggttgaccc ggatcaccag ttccttgccc ccgaacgcgt tgtcgcgcgc
      541 ctccttgatg ctgcggctgc gcaacaggcc gatcccgtcg aaggacgggg tgagcgaccc
      601 ttcgctggtg atctccagga cccggccgtt gcggcgcagc acgtgcggcc cgtcgatcga
      661 gaccatcgac accccgctgt cgccgacgat gcggacgcga cggccggagc aacggacgtc
      721 gagccgctcc actcccttgg ccccactggc gcgggcgccg ctgcggcgct gttcgtcccc
      781 gaacgccttc ggcggcggag gcggtgcata accgcggaag ccgtcgtcgg accggggccg
      841 ttcaccggcg agttcgtcgt cgctgggctc cgcacccggt ccgccggtcg cgtcgaccga
      901 accgctctcc gactgcagta cggcgatccg gcgggccgcc tcctcggtgt cgatccgccc
      961 cgcggccagg tcgtcgagga tgtgctgcat gtcgctcatg ccacgaatat agagcccgcg
     1021 cccccaccgg cgcgcccgtc cccggtgctt tcagggccat ccccgagcac gtgtcggggc
     1081 cgccacccca gtggatgggg ccactggtcg gtcagcgacg cctccccgac gtcggcagtc
     1141 accgccgtgc acaggtacgg cccgcgccgg gcagcagcga gctcgacggc cgaggcaagg
     1201 tcctgcgggt gcagcgccgc gaactcggtg tcggcacagg acggcacacc ggtcggctgc
     1261 gtcgccaccg ggttgcccca ctgcggccac cagtcctcac gggtcgggta ggccaaccgc
     1321 agcgtggtga cggtgagctg gggactgcgg gcagcctcgg cctcgcacgc tgtctcggcc
     1381 atccgcttgg tcagaccgta gaacgtggtg ttgtcggtct ctgccagcgc ggaccggcgc
     1441 cgctggccgt acccctccag cacggccatc gtcgagatct gcacgaaggc gggtacctga
     1501 gcacgtgccg cacagcgcag ctgggtgtag acgctgccga cgttgaccgc aaacccggca
     1561 cgcaccggct cggtcctttc gtcggcgccc gggccccgtg gggtccgcag cgcgaagttg
     1621 accactgcgt cgacaccgtc gaaggcatcg gccagcgcct cgaagtcggt cgcgtcagcc
     1681 accacgtggt cctccgttcg tccggccgga tccgggccga gcgtggtgac acggtggccg
     1741 gacgaccgca agtggggagt gatggcgctg cccaggacac cggcgccgcc gacaatcagg
     1801 atgtgcacaa tcgcaaatcc tgccatgtcg ccaaatcggt ccgggaacga accccaccgc
     1861 tgggcccggg gtgagggcac gatccgacaa cgatgcctcg cgaacccgct cccgggcatc
     1921 accgcacgtg gaccggagtg gcacagtgaa tgaagcagtt ccccggcctc ggagacaccc
     1981 tcatgaccga tccttctccc agccggcgac gcgcgctcgc cgcgttggcc acgctggccg
     2041 gagtgcccct catcggtggc tgcggtgcgc cgccggcccc gggcacctcc caacgcccgg
     2101 gcacgtcccc gaccggcacc accccg
//
LOCUS       DATGUK010000092         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1155255,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000092 DATGUK010000000
VERSION     DATGUK010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155255"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VK103_01460"
     CDS             complement(<1..227)
                     /locus_tag="VK103_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLQ82175.1"
                     /translation="MSESPVTPPGADSNQALARGLYVLRRLVESSTPLSATELAKELG
                     LHQSSISRVLATLIEAGYVRKDERGRFVPDYG"
     gene            560..1870
                     /locus_tag="VK103_01465"
     CDS             560..1870
                     /locus_tag="VK103_01465"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLQ82176.1"
                     /translation="MSTQSPAPAQTHPTMASVPFEPHEEVRIGLIGAGSRQRGLMRSL
                     SHVPGCRVVAVTDDSSEAAAAMVDRLTELGMPGAEPLSGPDAADVLCARDDVDLVMIA
                     TPWDSHAPMAVKAMRAGKHAAVEVPAAVTLEECWELVEVSEETRRHCVILENCCYGRT
                     ELLALQLVRAGALGQLLHAECSYTHDLRKMLLESKQWRRQAHIERDGNLYPTHGLGPV
                     ANCFGINRGDRFTRLVSMGSPSLGLQEWRAEHLPADDPRQQEEYVCADINTSLLQTEQ
                     GRTVVLQHQVVGPRPYDRRNQVVGTNGLIVDYPPRIYLEDDPLRAGDGTGEVGDDESE
                     MAEGEHFGPIEPYEQRFGHPLLADQGEKAQRAGGHGGMDYLMLLRLVQTMRRGEVPDM
                     DVYDAATWTAAGPLSEQSVAGGNVPVEFPDFTRGRWQEDRPGMP"
     gene            complement(1970..2422)
                     /locus_tag="VK103_01470"
     CDS             complement(1970..2422)
                     /locus_tag="VK103_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLQ82177.1"
                     /translation="MTTEERRIITATREIAAPADRIFELIADPARQPEWDGNANLATS
                     AEGQRVRAVGDVFTTTLTKGAVRQNHVVEFAEGRQIAWRPSEVDAPPFGHLWRWELEP
                     VGAGRTRVTQTYDWTTLTDEKRLARARATTEDNLLASIDRLAKVVEQG"
     gene            2750..>2924
                     /locus_tag="VK103_01475"
     CDS             2750..>2924
                     /locus_tag="VK103_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HLQ82178.1"
                     /translation="MTVIIALTANIGVAVAKSVVAALTGSASMLAEAAHSWSDSANEA
                     LLLVADKRSGKPPD"
BASE COUNT          447 a         1006 c         1064 g          407 t
ORIGIN      
        1 ccgtagtcgg ggacgaaccg gccacgctcg tccttgcgga cgtagccggc ctcgatcagg
       61 gtggcgagca cccgcgagat cgagctctgg tgcagcccga gctccttggc cagctcggtc
      121 gccgacaacg gcgtgctcga ctcgaccagg cgccgcaaca cgtacagtcc acgggccagg
      181 gcctggttgc tgtcggcgcc aggaggggtg acaggagact cactcactgg accatcatgg
      241 cggatcaggc agcgatgatg cagcgatgac gttgtcgagc cgccggcact cggtgacgaa
      301 gccctcgaag gtgtgcccgt agtacgcgcc aggacgttgc cggcatcatg cgcccggacc
      361 gccgcgaacg actcggccac cgcgggaagt atgccgactc cctccgccac tggccacggc
      421 atttcctcgc ccgcgaccca tcgtccgcac ccgtctcagg aagcgaagca cccccatcgg
      481 gacatcatca cccaccatga tggggcgggt ccgctccaca tcccgacggg cccggcccgc
      541 accgacacgg aagacagtga tgagcaccca gtcacccgca cccgcccaga cccatcccac
      601 gatggcctcg gtccccttcg aaccgcatga ggaggtccgg atcggcctga tcggtgccgg
      661 gagccggcag cgcgggttga tgcgcagcct gtcccacgtg ccggggtgcc gggtggtcgc
      721 ggtgaccgac gacagcagtg aggcggccgc ggcgatggtc gaccggctga ccgagctcgg
      781 gatgcccggc gccgagccgc tgtccgggcc ggacgccgcc gacgtgttgt gtgcccgcga
      841 cgacgtcgac ctggtgatga tcgccacccc gtgggacagc cacgcaccga tggcggtgaa
      901 ggcgatgcgg gccggcaagc acgccgcggt ggaggtgccg gccgccgtca ccctggagga
      961 gtgctgggag ctggtcgagg tctcggagga gacacggcgc cactgcgtga tcctggagaa
     1021 ctgttgctac ggccggaccg agctgctcgc cctgcagctg gtgcgggccg gggcgctggg
     1081 ccagctgctg cacgcggagt gctcctacac ccacgacctg cgcaagatgc tgctggagag
     1141 caagcagtgg cggcggcagg cccacatcga gcgggacggc aacctctacc ccacccacgg
     1201 gctcggcccg gtcgcgaact gcttcggcat caaccggggc gaccgcttca cccgactggt
     1261 gtcgatgggg tcgccgagcc tcgggctgca ggagtggcgc gccgagcacc tgccggccga
     1321 cgacccgcgg cagcaggagg agtacgtctg cgccgacatc aacacctcgc tgctgcagac
     1381 cgagcagggc cgcaccgtcg tgctgcagca ccaggtggtc gggccacgcc cgtacgaccg
     1441 gcgcaaccag gtggtcggca ccaacgggct gatcgtggac tatccgccgc ggatctacct
     1501 ggaggacgat ccgttgcggg ccggcgacgg gaccggtgag gtcggcgacg acgagtccga
     1561 gatggccgag ggggagcact tcgggccgat cgagccgtac gagcagaggt tcggccaccc
     1621 gctgctcgcc gaccagggcg agaaggcaca gcgggccggg ggccacggtg ggatggacta
     1681 cctgatgctg ctgcgactgg tccagaccat gcgccggggc gaggtgccgg acatggacgt
     1741 ctacgacgcc gccacctgga ccgcggccgg gccgctcagt gagcagtcgg tggccggcgg
     1801 gaacgtgccg gtggagttcc ccgacttcac ccgtggtcgg tggcaggagg accgtccggg
     1861 catgccgtga cgcccgggag catctcgcca tcaccgggag cacgtccgcg acggcgccgg
     1921 ggatgccgtc ggtgatcacg tccgggaggc gcgcgcccgt accgccgggt cagccctgtt
     1981 cgacgacctt ggcgagccgg tcgatcgagg cgagcaggtt gtcctcggtc gtcgctcggg
     2041 cacgggcgag ccgcttctcg tcggtgagcg tggtccagtc gtaggtctgg gtgacccggg
     2101 tccggcccgc gccgaccggc tccagctccc aacgccacag gtgcccgaat ggcggggcat
     2161 cgacctccga cggtcgccac gcgatctgcc ggccctcggc gaactcgacg acatggttct
     2221 gccggaccgc ccccttggtg agcgtggtgg tgaacacgtc accgaccgcc cggacccgct
     2281 gcccctccgc ggaggtggcc aggttggcgt tgccgtccca ttcgggctgc cgtgccgggt
     2341 cggcgatcag ctcgaagatg cggtcagcgg gtgcggcgat ctcgcgggtc gcggtgatga
     2401 tgcggcgttc ctcagtggtc atggtgatga ttcaaccact ggcatccgcg ggctcgatcc
     2461 ggacgccccc gctgagtcat accgctgcgg gccggaccac gacctgcacg atgccggcac
     2521 gtcgacctac gtgtcgtcga gcacatggtc gggtcggcac gactgctcgc cggttcgtac
     2581 ggcttcgacg aacaggacgc acgcgctcgg tgaggtctcg gggtgccccg ggtccccggg
     2641 ggaacgccgt cgggtgcaag ctggactggt gagcgatgcc gcaggggacc gggatgcccg
     2701 tggccgggcg caggaccccg atgcgcagca ggggtcgggc aaggtcagcc tgaccgtgat
     2761 catcgcgctg acggccaaca tcggcgtggc ggtggcgaag tcggtcgtcg ctgcgctcac
     2821 cggatcagcc tccatgctgg ccgaggcggc ccactcctgg tccgacagcg ccaacgaggc
     2881 cttgctgctg gtggccgaca agcggtccgg caagccgccg gacg
//
LOCUS       DATGUK010000093         3624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1388802,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000093 DATGUK010000000
VERSION     DATGUK010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388802"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VK103_01480"
     CDS             <1..744
                     /locus_tag="VK103_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HLQ82179.1"
                     /translation="VASDHHPVAVLGLTAVFIVLLVVSIMITRTPEALDPVSRRHERV
                     RRLGLVLALGIPAQAVFGGITVMTRLNPWIVAGHLLVTFVLIAVAMWLVHTAGPAPRP
                     AVGRTGWLLSRLVAVALAVSMWLGTVVTGSGPHSGDETARRTGLDPELMSHIHASAVY
                     LTIGLTLAAVLVLRSRAAMLLLVLEVAQGVVGLVQYWTGLPIVVVMVHMLGSAVLVAA
                     GTHLVWTTGRERPTPEVPVESTREVFHAR"
     gene            1007..2524
                     /locus_tag="VK103_01485"
     CDS             1007..2524
                     /locus_tag="VK103_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HLQ82180.1"
                     /translation="MRWVDELATSGLPDNDAERVELLSALERLSNAARATQARVTVGL
                     DSSQRSAQAADGVPAARRGRGIAGQVALARQESPHRGQQLHQLALVLDRELPATADAF
                     RRGLLSQRRAQIIARETAVLTAAHRARVDREVAGDPDRLAGKGDRALAGECARWAARL
                     DPASVVARRRRAERERHVGLRPAPDTMSRLSALLPVTTGVAVYGALVGAADAARAQGD
                     PRSRGQVMADTLVARILGSDARSSGRAAGAQHRPTRAGGTPAGGGAPISWEDCDIARP
                     PAAHDSRTRHDSPTTPTAGHDPATDSLPSSLPESVGVVLNVVLTDAQLFGDADGAAWL
                     DGHGPVDAELARELAHHGRARVRRAFADPATGSLVGLESGSRLFPAGLRSLVRLRDGS
                     CRTPWCDAPVRHIDHVTAHRDGGATTFRNGQGLCESCDYTKQAPGWTAEPVPAAPPGQ
                     PAGTPHDVVTTTPTGHRYRSPTPSRVAPATAGSGSTDPTVELYLPPFAVEVVLAA"
     gene            complement(2407..3459)
                     /locus_tag="VK103_01490"
     CDS             complement(2407..3459)
                     /locus_tag="VK103_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLQ82181.1"
                     /translation="MSHQRGLSLPHRTGGVSMVGTLIALASAMSFAVSGSFAKGLLDA
                     GWSPGAAVTVRIVGAALVLAPALLLVPGDLRRLPRAAWPVIGYGALGVAGAQLCYFMA
                     LDTLPVSVALLIEYSAPVLIVGWTWARSGRRPNGWVLIGTVVAVTGLVAVIDPAGATL
                     DPVGLAWASGAGLCNAGYFLLSARAVDGIAPLTMTAAGVGVAGLGIGATAALGLLPVT
                     MTFGSVTLAGAAVPWWLPAVGLVLVSTVLAYGTGLLAARRLGAQLASFVALTEVVFAV
                     LAAWVLAGEVPGLPQLVGGVVMLAGVATVRAGSVTDQAASTTSTANGGRYSSTVGSVE
                     PEPAVAGATREGVGLR"
BASE COUNT          558 a         1373 c         1255 g          438 t
ORIGIN      
        1 gtcgccagcg atcaccaccc ggtggccgtg ctcggcctga cggcggtctt catcgtgctg
       61 ctggtggtgt cgatcatgat cacccgcacc cccgaggccc tcgacccggt gagccgtcga
      121 catgagcggg tgcgacgtct gggactggtg ctggcgctcg ggatccccgc ccaggcagtg
      181 ttcggtggca tcacggtgat gactcggctg aacccgtgga tcgtggccgg ccacctgttg
      241 gtcaccttcg tgctgatcgc ggtggcgatg tggctggtac acacggccgg cccggcaccc
      301 cggcccgccg tgggccgtac cggctggctg ctgtcccgac tggtggcggt cgccctcgcg
      361 gtgtcgatgt ggctgggcac cgtggtcacc ggctcgggcc cgcactccgg tgacgagact
      421 gcacgccgga ccgggctcga ccccgagttg atgagccaca tccatgcgag cgcggtgtac
      481 ctgaccatcg gcctgacgct cgcggcggtg ctggtgctgc ggagccgggc ggcgatgctg
      541 ctgctggtgc tggaggtcgc ccagggcgtg gtcgggttgg tccagtactg gacggggctg
      601 ccgatcgtgg tggtgatggt gcacatgctc ggctcagcgg tcctcgtggc cgccggcacc
      661 cacctggtgt ggaccaccgg gcgggaacgg ccgacacccg aggtcccggt ggagtcgacc
      721 cgcgaggtct tccacgcccg ctgagtcggg ccgcccggcc gccacgactg agtagctcga
      781 gatttctcga gcaactcctt gcctgtgaac ggatgttcga ttacaatcga acacaggcgc
      841 tggaggcacg gaaggcggag acacagaagc ggagacacgg aagcggagac acggaggagg
      901 gggagcgatg acacgtgatt cgcaaccagg cccgccgtgc cgccctgcgg ccccaacagc
      961 cccctccccc ggacaaggcg ccaatggcat ccctgccgcg accgtgatgc gatgggtcga
     1021 cgaactcgcc acatctggcc tcccggacaa cgacgccgag cgcgtggagt tgctgtcggc
     1081 actggagcgc ctcagcaacg ccgcccgggc gacccaggcc cgcgtgacgg ttggtctcga
     1141 ctcctcgcag cgctctgcac aggccgccga cggggtcccg gcggcacgcc gaggtcgcgg
     1201 gatcgcgggc caggtggcac tggcgcgaca ggagtcaccc catcgcggcc agcagctcca
     1261 ccagctcgcc ctggtcctgg accgtgagct cccggcgacg gcggacgcct tccgccgcgg
     1321 cctgctctcg cagcgccggg cccagatcat cgcccgcgag accgctgtgc tcaccgcggc
     1381 gcaccgggcc agagtggatc gcgaggtcgc gggtgacccg gaccgcctcg caggcaaggg
     1441 cgaccgagcc ctcgccggcg agtgcgcccg gtgggccgcc cgcctcgacc cggccagcgt
     1501 ggtcgcgcgt cgccgccggg cggagcgtga gcgtcacgtc gggctacgac cggccccgga
     1561 caccatgtcc cggctgtcgg ccctgctgcc ggtgaccacg ggggttgcgg tgtacggggc
     1621 actggtcggc gccgccgacg cggcccgggc ccagggggac ccacgatcgc gcggccaagt
     1681 gatggccgac accctggtcg cgcggatcct cggatctgac gcacgctcat ccggtcgtgc
     1741 ggccggtgcg caacatcgac cgacccgtgc cggaggcacc cccgcagggg gcggcgcgcc
     1801 gatcagctgg gaggactgcg acatcgcccg tcccccggcc gcacacgatt cgcgcaccag
     1861 acatgactcc cccacgaccc cgactgctgg ccacgacccc gccaccgaca gcctcccctc
     1921 cagcctgccc gaaagcgtcg gcgtggtgct caacgtcgtg ctgaccgacg cacagctgtt
     1981 cggcgacgcc gacggagccg cctggctgga cggccacggc ccggtcgacg ccgagctcgc
     2041 tcgcgagctc gcccaccacg gccgggcccg ggtccgcaga gccttcgccg acccggccac
     2101 cggatcgctg gtcggcctgg agtccggctc ccggttgttc ccggccggcc tgcgatcgtt
     2161 ggtccgactc cgggacggca gctgccgcac cccctggtgc gacgctccgg tccgccacat
     2221 cgaccacgtc accgcacacc gggacggcgg agccaccacg ttccgcaacg gccaagggct
     2281 ctgcgagagc tgcgactaca ccaagcaggc ccccggctgg accgcggaac cggtgccggc
     2341 ggcaccaccc ggacagccgg ccggcacacc gcacgacgtc gtcaccacca ccccgaccgg
     2401 gcaccgctac cgaagtccca cgccctcccg ggtggctccg gcaaccgccg gctcgggctc
     2461 gaccgacccc acggtcgagc tgtacctgcc gccgttcgcc gtcgaggtcg tgctggccgc
     2521 ctgatcggtc accgagccgg cgcgcacggt ggcgaccccg gcaagcatca ccacgccgcc
     2581 gaccaactgg ggcaggcccg gcacctcgcc cgccagcacc caggcggcca acacggcgaa
     2641 caccacctcg gtgagcgcca cgaagctggc gagctgggcg cccaaccggc gcgcggcgag
     2701 cagcccggtg ccgtacgcca gcaccgtcga gaccagcacc aggcccacgg cgggcagcca
     2761 ccacggcacg gccgcaccgg ccagcgtcac cgagccgaag gtcatcgtca ccggcaacag
     2821 gccgagcgcc gccgtggcac cgatcccgag cccggcgacg ccgaccccgg cagcagtcat
     2881 ggtgagcggg gcgatcccgt cgaccgcccg tgccgacagc aggaagtagc ccgcattgca
     2941 cagcccggcc ccgctggccc atgccaggcc gaccggatcg agggtggccc cggccggatc
     3001 gatcacggcc accagtccgg tgacggcgac gacggtgccg atcagcaccc agccgttggg
     3061 gcgccggccg gaccgggccc aggtccagcc gacgatcagc acgggcgcgc tgtactcgat
     3121 cagcaacgcc accgagaccg gcagggtgtc gagtgccatg aagtagcaca gctgcgcccc
     3181 ggcgacgccg agtgcgccgt aaccgatgac cggccacgcc gcccggggca gccgccgcaa
     3241 gtcccccggc accagcagca gggcgggcgc gagcaccagg gccgccccca cgatccgtac
     3301 cgtgactgcg gcgccgggcg accacccggc atccagcagc cccttggcga acgatccgga
     3361 caccgcgaac gacatcgccg acgccagggc gatcagggtg ccgaccatcg acacacctcc
     3421 cgtacggtgg ggcaggctca ggccgcgctg gtgggacacc ggtcacctcg tcagacgtct
     3481 ggaacgatcg gcggcgcggc agggtcgcga cactgggatt gacgggcacg ccgccggaag
     3541 gaagtacagt catgagtgat catgctggac actcatgaca ctggcgctca ggctagactc
     3601 cacaaccgac aggaatcaaa ttgg
//
LOCUS       DATGUK010000094         4988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_106033,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000094 DATGUK010000000
VERSION     DATGUK010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106033"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VK103_01495"
     CDS             <1..367
                     /locus_tag="VK103_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82182.1"
                     /translation="AILPRGHVATVFLGLLLVWALFAGPAPPWWYLVVALGWLGVHWA
                     AGACAVGPSFADVDPAVWRGLAVPAVWAAIGIVVATVLAVVAELVTLPSSLVLVVLTV
                     GILIVGAAIVLTPGRQTDR"
     gene            599..1129
                     /locus_tag="VK103_01500"
     CDS             599..1129
                     /locus_tag="VK103_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82183.1"
                     /translation="MADVDADVDADVDDAQLWSPAAREGYAQAVERVHRALDRHNVAI
                     AGSTDTTGLAASAAELEAALASLSDAEHALTGAAAFWLDLDDPADDATDEPSHGDDQG
                     DESIPVVAQLQLTMDVTDTDRLLALARQLDPAAPVSSMEDAVFVVTGRLGWDQLLADQ
                     IDGVTMQGATARLDTP"
     gene            complement(1148..2188)
                     /locus_tag="VK103_01505"
     CDS             complement(1148..2188)
                     /locus_tag="VK103_01505"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HLQ82184.1"
                     /translation="MATPAVPGPVDRLILDESVGLPADRITVLDDPDGHLTAAVARRS
                     PGATVVVHTDSLRDERAVAGHCAAQDVSITVATDLAAAVTDTDLVLLRLPRSVDALTE
                     TAEVTARLAAPRVALVGGGRSKHLSHGLNDALARGFGSVRASLGRDKSRVLHARDRHA
                     DVADRWPRRRTDPELGLVIHAHGGVFAGTRVDRGTRLLLSVLADRTAPRGPVLDLGCG
                     SGVLAATIARRWPDVPVLASDVSAAAVASAALTTGGAVRCRRADGLTDWGPESLGAIV
                     TNPPFHVGAAKDSTPTMRMIADAGRVLRPGGELFMVYNAHLPYLPRLRRVGPTRIVAR
                     DRHYVVTHTVRD"
     gene            complement(2235..2846)
                     /locus_tag="VK103_01510"
     CDS             complement(2235..2846)
                     /locus_tag="VK103_01510"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HLQ82185.1"
                     /translation="MFFSDAVIAIALTLLILPLMESVYEIDGGSTAAWVLAEAGPLIN
                     FLVSFAVIATMWSFHHGSFARVRAVTRPLLLLDLGWLATIVFLPVATALTNATEPDVL
                     QHLFYIGTMLLSSVLLVAINTVITRDRRVTDVPGGNLGHLAAALAMAACLSAALLVAC
                     LTPLNDAAMTLMLLVAPLQRMISRPLRRRGLPGPDPAGRESDT"
     gene            3149..3940
                     /locus_tag="VK103_01515"
     CDS             3149..3940
                     /locus_tag="VK103_01515"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HLQ82186.1"
                     /translation="MLPLDHHALTHQGCVRRNNEDSVLAQAPVFVVADGVGGAEAGEV
                     ASGIVVEEFARLLPGPVDDAAAGRALQRAHNRVLHHNERNDTHAATTAAGAVALSMGA
                     GQHYWLFFNIGDSRVYRRNGPLGHSLVQISVDHSRVQEMLSSGQIGPAEARQHPERNV
                     VTRAIGAEAEICPDFWLLPMHPGERVVICSDGLLGDSPGSEVKFLVKEVPGAETAARH
                     LVDLALRHGARDNVSVIVIDVPEVTGEVDTAAPMAQTVLTARRNP"
     gene            3937..>4988
                     /locus_tag="VK103_01520"
     CDS             3937..>4988
                     /locus_tag="VK103_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82187.1"
                     /translation="MTEPTPTAPAATDGGTVGQWRATYTPGSWVVLSGPTSMVVLQPA
                     PAKASTLLNSMWDVVVSAASVDQLVERLAQFNVGRMPSFAAFFWHDGQMRSLVRGAVS
                     VMDLTSGEYVADGEGIQTWSEIGLGDVRQVRIDMEGTPEESVDKLQLPLVVGAVTASA
                     IVLDATDEVRVVSPQAVGAEPALAPTDPRVDRMSAEQAGAEQGAPEQGGTGAGDTEAG
                     SSDGVPVGAAVAAGAAAAGATGTDATDTDATDTDATDTETGGSDASGTDADGSAAEAP
                     SAAAAGTGGRPVDPAVALFGGAPAAAETGDPTAAPIADADQQPGPPASQPSAPETPQP
                     AAAQSSASEPPAAAPEP"
BASE COUNT          683 a         1730 c         1879 g          696 t
ORIGIN      
        1 cgcgatcctg ccgcgggggc acgtggccac cgtgttcctc gggttgctgc tggtgtgggc
       61 gttgttcgcc ggtccggcgc cgccatggtg gtacctcgtc gtcgcgctcg gctggctcgg
      121 ggtgcactgg gccgccggtg cgtgtgcggt cggccccagc ttcgccgatg tggacccggc
      181 ggtgtggcgt gggctggcgg tgcccgcggt gtgggctgcg atcggcatcg tggtcgccac
      241 ggtgctcgcc gtggttgccg agctggtcac gctgccctcg tcgctggtgc tggtggtgct
      301 gaccgtcggc atcctcatcg tcggcgcggc gatcgtgctc acccccggtc ggcagaccga
      361 ccgctgacgc cacgcgcccg cacggcaggt cctcccggcc acggcagttg cgcccggtca
      421 cggccgttgc ttctccgcac ggcaggtgta tcggccgcgg ctgccaccaa tggccgtgcg
      481 acattgaagc tcgtgggtct gcggacactg ccgcccgccg gtgcggcacc gggcgcccac
      541 gcgagcggtc gattcgcgga atccggctgc ccacccaggg tggccgcgcc gcgatagcgt
      601 ggccgatgtg gatgccgacg tggacgccga cgtggatgat gcccagctgt ggagcccagc
      661 ggcccgtgag gggtacgcgc aggcggtcga gcgggtgcac cgcgcgctcg accggcacaa
      721 cgtggcgatc gccggcagca ccgacacgac tggcctggcg gcgtcggcgg ccgagttgga
      781 ggccgcactg gcgtcactca gtgacgccga gcatgccctc accggggccg cggccttctg
      841 gctcgacctc gacgacccgg ccgacgacgc gaccgacgag ccgtcccacg gtgatgacca
      901 gggtgacgag tcgatcccgg tggtcgccca gctgcagctg accatggacg tcaccgacac
      961 cgaccggctg ctcgccctgg cccggcagct cgatccggcg gctccggtgt cgtcgatgga
     1021 ggacgcggtc ttcgtcgtca ccggacggct cggctgggac cagctgctgg ccgaccagat
     1081 cgacggtgtc accatgcagg gggcgaccgc ccgcctcgac accccgtgac ccgacctcag
     1141 tgaggcgtca gtcccggacg gtgtgcgtca ccacgtagtg ccggtcgcgc gcgacgatcc
     1201 gtgtcgggcc cactctccgc agtcgcggca ggtacggcag gtgggcgttg tagaccatga
     1261 acagctcacc gccgggccgc aacacccggc cagcgtcggc gatcatccgc atcgtcggcg
     1321 tggagtcctt ggcggcaccg acgtggaacg gcgggttggt cacgatggct ccgagcgact
     1381 ccggccccca gtccgtgagc ccgtcggccc gccggcaccg tacggctccg ccggtggtca
     1441 gtgccgccga cgcgaccgcg gccgcggaga cgtcactggc cagcaccggg acatccggcc
     1501 accgtcgtgc gatcgttgcg gccagcacgc cggagccaca gccgaggtcc aacaccggtc
     1561 cgcgcggtgc ggtccggtcg gccagcaccg acaacagcag ccgggtgccg cggtccaccc
     1621 gggtgccggc gaacaccccg ccgtgggcgt ggatcaccag gcccagctcc gggtcggtac
     1681 ggcgtcgtgg ccaacggtcc gcgacgtcgg catggcggtc ccgggcgtgc agcacccgtg
     1741 acttgtcccg gccgaggctg gcccggaccg agccgaagcc gcgggcgagt gcgtcgttga
     1801 gtccgtggct caggtgcttg ctgcggccgc cacccaccag tgccacccgt ggagcggcga
     1861 gtcgggcggt gacctcggcg gtctcggtga gcgcatcgac ggagcggggg agtcgcagca
     1921 gcaccaggtc cgtgtcggtg accgcagctg ccaggtcggt cgcgacggtg atcgacacgt
     1981 cctgggcggc gcagtggccg gcgaccgccc gctcgtcccg cagcgagtcg gtgtgcacca
     2041 cgacggtcgc accggggctg cgccgggcga cggcagcggt gaggtggccg tcggggtcgt
     2101 cgagcaccgt gatccggtcg gcgggcaggc ccaccgactc gtccaggatc agccggtcga
     2161 ccggaccggg caccgcggga gtcgccaccg cgccatcgtg ccgcgccggg aacggcggca
     2221 ccaatccggg ccggtcaggt gtcggactcc cgtccggctg gatccgggcc ggggagccct
     2281 cggcgtcgca gtggccggga gatcatccgt tgcagtggtg ccaccagcag catcagcgtc
     2341 atcgctgcgt cgttcagcgg ggtgaggcag gcgaccagca gtgcggccga caggcaggcc
     2401 gccatcgcca gcgcggcggc caggtgcccg agattgccac cggggacgtc ggtgactcgt
     2461 cggtcgcggg tgatcacggt gttgatcgcg accagcagca ccgagctgag caacatggtg
     2521 ccgatgtaga acaggtgctg cagcacatcg ggttcggtgg cgttggtgag tgctgtcgcg
     2581 accggcagga acacgatggt cgccagccat ccgaggtcga gcagcagcag tggccgggtg
     2641 acggctcgca cccgcgcgaa gctgccgtgg tggaacgacc acatcgtcgc gatgaccgcg
     2701 aaggagacga ggaagttgat caatggtccc gcctccgcca ggacccaggc ggcggtgctg
     2761 ccgccatcga tctcgtacac cgactccatc aacggcagga tcaacagcgt caatgcgatc
     2821 gcgatgacgg cgtcggagaa gaacaccagt cgttccgggt tgtgtacggg gttccggccg
     2881 ggtggggtgg tggtcgcggc ggatgccgac gcagcgggtg gcgcctgccg aggtgccgcg
     2941 tgcgtcgagg gtgcggtgcc tccggcgggc ccgtcggagc tccccgtgct ggtgtccgcc
     3001 gttccgggcg gggtgccacc cggatgtggc ggggtcccgg cgttgtcggc ggtgtccacc
     3061 atcgcaccct actgccgggt cccgaccgtc cggaccgcca tcgcgaccgg tgcggtcgcc
     3121 gacccctggg aaccctagac tcggccccgt gctcccactc gaccaccatg ccctgacaca
     3181 tcagggctgc gtccggcgca acaacgagga cagtgtgctg gcgcaggcgc cggtgttcgt
     3241 ggtggctgac ggagtcgggg gcgccgaggc cggcgaggtc gccagcggga tcgtggtgga
     3301 ggagttcgcc cgcctgctgc ccggcccggt ggacgacgca gctgccggcc gtgccctgca
     3361 acgggcccac aaccgggtgt tgcaccacaa cgagcgcaac gacacccacg cggcgaccac
     3421 ggcggccggt gcggtggcgc tgtcgatggg ggcgggacag cactactggc tgttcttcaa
     3481 catcggggac tcccgcgtct accgtcgcaa cgggccgttg gggcactcgc tggtgcagat
     3541 cagcgtcgac cattcccggg tgcaggagat gctgtcgtcc gggcagattg gtccggccga
     3601 ggcccggcag catccggagc gcaacgtggt gactcgggcg atcggggccg aggccgagat
     3661 ctgcccggac ttctggctgt tgccgatgca ccccggtgag cgcgtggtga tctgctccga
     3721 cgggctgttg ggcgacagcc ccggctcgga ggtcaaattc ctggtcaagg aggtccccgg
     3781 agcggagaca gcggcccgcc acctggtgga cctggcgttg cgccacgggg cgagggacaa
     3841 cgtgtccgtc atcgtgatcg atgtcccgga ggtgaccggt gaagtcgaca ccgccgcccc
     3901 gatggcacag actgtgctga cggcgaggag gaatccatga ccgaacccac cccaaccgcc
     3961 cccgcggcca ccgacggcgg aaccgtcggg cagtggcggg cgacctacac ccccggttcc
     4021 tgggtggtgc tgtccggccc gacctcgatg gtggtgctgc agcccgcccc ggcgaaggcg
     4081 tcgacgctgc tcaactcgat gtgggacgtg gtggtgtccg cggcctcggt cgaccagctc
     4141 gtcgagcggc tggcccagtt caacgtcggc aggatgccgt cgttcgcagc gttcttctgg
     4201 cacgacggcc agatgcggtc gctggtccgc ggggccgtca gcgtgatgga cctgaccagt
     4261 ggtgagtacg tcgccgacgg ggagggcatc caaacctgga gcgagatcgg gctgggggac
     4321 gtgcggcagg tccggatcga catggagggc acgccggagg agtcggtcga caagctgcag
     4381 ctcccgctgg tggtcggggc ggtgaccgcc tccgccatcg tgctggacgc caccgatgag
     4441 gtacgggtcg tctccccgca ggcggtcggc gcggagccgg ccctcgctcc cacagacccc
     4501 cgtgtcgatc ggatgagcgc cgagcaggcg ggtgccgagc agggtgcccc cgagcagggt
     4561 ggcaccgggg ccggtgacac cgaggccggg agctccgatg gggtcccggt cggtgccgcc
     4621 gttgcagccg gagcggccgc ggcaggtgcc accggcacag atgccactga cacggatgcc
     4681 accgacacgg atgccaccga cacggagacg gggggctcgg acgccagcgg taccgacgcc
     4741 gacggatccg ctgccgaggc gccgtcggcg gcagccgccg gcaccggtgg ccggccggtc
     4801 gaccctgcgg tggcgctgtt cgggggcgcg ccggcggcgg ccgagaccgg tgaccccacc
     4861 gctgcgccca tcgccgatgc ggaccagcag cccgggccgc cggcgtcaca accgtccgcg
     4921 ccggagacac cccagcctgc ggcagcccag tcctcggcgt ccgagccgcc ggcagcggca
     4981 ccggaacc
//
LOCUS       DATGUK010000095         3413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1390954,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000095 DATGUK010000000
VERSION     DATGUK010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390954"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="VK103_01525"
     CDS             complement(<1..1021)
                     /locus_tag="VK103_01525"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLQ82188.1"
                     /translation="MADPEVWDTVVVGGALAGLAAAARLAKNRHRVLVLEAHDRLGGR
                     LAPHPLGTGGDAVPLVDSWPGMITFPAPWRDLFRKSGRPFVDELARTDQELAPAPATR
                     HVFTESVGGARDLVLPSERGVQFSVLSQRYGVTVAERWRDLLDHLDEVAQALRPLGGE
                     AELTSYEQVTAARSVLSPGRSVADLARALDHPHLSALLTSTAWRLGSRPGRTPGWVAV
                     RLANERRFGRWMVTTDGRPDRTSVLLDRLEGRLRTRRVTVRTDHTVTGVTSAGDGGLT
                     VTAETETGPVVEQARTVVLSVHPGRAYRLLGRAAGAERRRAARTRPAWAPVVSHIVSD
                     GAGSEG"
     gene            complement(1014..1649)
                     /locus_tag="VK103_01530"
     CDS             complement(1014..1649)
                     /locus_tag="VK103_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HLQ82189.1"
                     /translation="MELLPLALVSGWACGVNTWLTVLVLGVLGRFAAFAEVPELVQRT
                     DVLVVVGLLALVELVADKMPFLGTAWDVVGTVLRPVAGGVVGALLGGADGSVGTVVLA
                     VVGALAALLSHGVTSVLRLGTTRSGEPARTIAIAVASDLAVGFAVVAAVLVPVVVAVV
                     VLAVLALTVLLALRLRHRAAEGWRAFRRWLGSRLTGFRRRIPTRRGGDRDG"
     gene            1796..2686
                     /locus_tag="VK103_01535"
     CDS             1796..2686
                     /locus_tag="VK103_01535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLQ82190.1"
                     /translation="MEVGHDDTTAGTDGAGRVSPATGPSGGRIHLGGAQQTMLGTLWC
                     RAVDARRRRPILGDHAAVHAVDRIDHPFHRLGVDAMTVAVTCTRARWMDERVARFLAG
                     HARATVLHLACGLDTRQQRLRPGPGVQWFEVDQPDVIAVRRQVLPPPPGVELVDADVT
                     DSGWFAAVPQDRPTLVVAEGLSMYLDPERGRALLTRLASTLRGELIMDFNGRAGMVGQ
                     VLNRTVRRSRARWTWLVDEPGSLQEHGPVLQESVPLIDLLRALDPRQLPLPRWLALQG
                     SRLTPAPPAGLLARYGLPGS"
     gene            2816..>3413
                     /locus_tag="VK103_01540"
     CDS             2816..>3413
                     /locus_tag="VK103_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HLQ82191.1"
                     /translation="MSAPPRTPVSRRNLITAGAATTAAALVTRPTSSAHAAAPRTDPF
                     TLGVASGDPTADGIVLWTRLAVDPLAEDGLGGMAPSAVEVEWEIATDERFSTVVQRGT
                     EQAVRGWAHSVHAEVDGLEPSTDYWYRFRAGSHVSPAGRTRTAPRRNGHDPLNLALVS
                     CSHYAWGHWTAYRHLAEEQPDLVLHAGDYFYEGQFVEGQ"
BASE COUNT          487 a         1273 c         1234 g          419 t
ORIGIN      
        1 atccttccga cccggcccca tcgctcacga tgtggctcac cacgggcgcc caggcgggac
       61 gggtccgcgc agcccggcgg cgctcggcgc ccgcggctcg tcccagcagg cggtaggccc
      121 gtccggggtg cacggagagc acgaccgtac gggcctgctc caccaccgga ccggtctcgg
      181 tctcggcggt gacggtgagc ccaccgtcac cggcactggt gacaccggtg acggtgtggt
      241 cggtgcggac cgtgacccgc cgggtccgca gcctgccctc caaccggtcc agcagtaccg
      301 aggtgcggtc cgggcgcccg tcggtggtga ccatccaccg gccgaagcgg cgctcgttcg
      361 ccagccgcac cgccacccaa ccgggggtgc ggcccggccg ggagcccagt cgccacgcgg
      421 tgctggtgag cagcgcagag aggtgcgggt ggtcgagtgc gcgggccagg tcggcaacgc
      481 tgcggccggg ggacagcacc gaccgtgccg cggtgacctg ctcgtacgag gtcagctcgg
      541 cctctccgcc gagcggccgc agcgcctggg cgacctcgtc gaggtggtcc agcaggtcgc
      601 gccaccgctc ggcgacggtg acgccgtacc gctgggacag cacggagaac tgcaccccgc
      661 gctcactggg cagcaccagg tcgcgcgcgc ctccgaccga ctcggtgaac acgtgccggg
      721 tcgccggcgc cggcgccagc tcctggtcgg tgcgggcgag ctcgtcgacg aatgggcgcc
      781 cactcttgcg gaacaggtcg cgccacggtg ccgggaaggt gatcatgccc ggccaggagt
      841 cgaccagcgg cacggcgtcg cccccggtcc ccagcgggtg cggggcgagg cgaccgccga
      901 gccggtcgtg cgcctccagc acgaggaccc ggtgacggtt cttggccagc cgtgccgcgg
      961 ccgccaagcc ggccagtgca ccgcccacca cgacggtgtc ccagacctcg gggtcagcca
     1021 tcccggtccc ctccgcggcg ggtcgggatg cgtcgtcgga acccggtgag ccgggagccc
     1081 agccagcggc ggaaggcgcg ccagccctcg gcggcccgat gccgcagccg cagcgccagc
     1141 agcacggtca gcgccagcac ggccagcacc acgaccgcca ccaccaccgg gaccagcacg
     1201 gcggcgacga ccgcgaaccc caccgcgagg tcactggcca ccgcgatcgc gatggtgcgg
     1261 gcaggctcac ccgatcgggt ggtccccaac cgcagcacac tggtgacccc gtggctcagc
     1321 agcgcggcga gtgccccgac cacggcgagc acgacggtgc cgacactgcc gtccgcgccg
     1381 cccagcaggg ccccgaccac gccaccggcg acggggcgga gcacggtgcc caccacgtcc
     1441 cacgcagtgc cgaggaaagg catcttgtcc gccaccagct cgaccagggc gagcaggccg
     1501 accaccacca ggacatccgt gcgctgcacg agctcgggga cctcggcgaa cgcggcgaac
     1561 ctgcccagca cgcccagcac cagcacggtc aaccaggtgt tcacgccgca ggcccagccg
     1621 ctgacgaggg cgagcgggag cagttccatg cggccgagcg tacttggggc gggccgtgcc
     1681 ggcccggggt gcgtgcgaac cgatggcgtc accgtcagct gggcggcgac tcgtgccggg
     1741 ccccggagca caatgacggc ccggagcaca atggcaccca gcaggggtga ctccgatgga
     1801 ggtgggccat gacgacacca cggctgggac ggacggagcg ggacgggtga gcccggccac
     1861 cggcccgtcc ggcgggcgca tccacctcgg cggcgcgcag cagacgatgc tgggcaccct
     1921 gtggtgccgg gccgtcgacg cacgtcgcag gcgcccgatc ctgggcgacc acgccgctgt
     1981 ccatgccgtc gatcgcatcg accacccgtt ccaccgactc ggtgtcgacg cgatgacggt
     2041 ggcggtgacc tgcacccggg cccgctggat ggacgagcgg gtggcccgct tcctcgccgg
     2101 gcatgcccgg gccaccgtgc tccacctggc ctgtggcctg gacacccggc agcagcggtt
     2161 gcgacccggg cccggcgtgc agtggttcga ggtggaccaa ccggacgtga tcgcggtccg
     2221 gcggcaggtg ctgccaccgc caccgggagt ggagttggtc gacgccgacg tcaccgactc
     2281 cggatggttc gccgcggtgc cgcaggaccg ccccaccctg gtggttgcgg aggggttgtc
     2341 gatgtacctc gatccggagc gtggacgagc actgctgacg cgactcgcca gcaccctccg
     2401 cggtgagttg atcatggact tcaacggccg tgccggcatg gtgggtcagg tcctgaaccg
     2461 caccgtgcgc cggtcccggg cacggtggac ctggttggtc gacgaaccgg gctcgctgca
     2521 ggagcacggg ccggtgttgc aggagtcggt gccgctcatc gacctgctcc gagcgctcga
     2581 tccgcgacag cttccgctgc cgagatggct tgccctgcag ggatcccggc tgactcccgc
     2641 cccacccgca gggttgctcg cccggtacgg gctgcccggc agctgaggtg gtcgggcgcg
     2701 accactgctg gcgcacgatg cggccgtggg ctatcgcggt attgcggttt gccgattgcc
     2761 gaaccgcgac ggtggtcccc accatggagg gcgtccccga tgaggaggag cccgcatgtc
     2821 cgcaccgccc cgtacccctg tcagtcgccg gaacctgatc accgccggtg cggccaccac
     2881 cgctgctgcg ttggtcaccc gccccacgtc atcggcgcac gcggccgctc cccgtaccga
     2941 cccgttcacc ctcggggtgg cctccggcga tccgaccgcc gacggcatcg tgctgtggac
     3001 ccgactggcc gtcgacccgc tcgccgagga cggcctcggc gggatggcgc cgtcggcggt
     3061 ggaggtggag tgggagatcg ccaccgacga acggttctcg acggtggtgc aacgggggac
     3121 cgagcaggcc gtacggggct gggcccacag cgtgcacgcc gaggtcgacg gactcgagcc
     3181 gtccactgac tactggtacc ggttccgtgc cggcagccac gtctcgcccg ccggccgcac
     3241 ccgcaccgcg cctcggcgga acggccacga cccactcaac ctggcgctgg tgtcgtgctc
     3301 gcactacgcg tgggggcact ggaccgccta ccggcacctg gccgaggaac agccggacct
     3361 ggtgctgcac gccggggact acttctacga gggccagttc gtcgagggcc aac
//
LOCUS       DATGUK010000096         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_109990,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000096 DATGUK010000000
VERSION     DATGUK010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109990"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VK103_01545"
     CDS             <1..677
                     /locus_tag="VK103_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HLQ82192.1"
                     /translation="SGGAYGIDVAAHRGALVVPAGRTLAVLACGPDIEYPRGNLLVLQ
                     RLAEHHLVVSEVPPGIRPTRIRFLARNRLIAALAAGTVMVEAAARSGARNTMAWTEAL
                     GRVAMAVPGSVHSALSVGPLHLVRDRRAELVTGPEHVLELLAPMGSHQTTDPRGARTR
                     LDALSPEVAAVYEALPARGYRTAGEVALATGRTVPDSLAALAALELQGLAEPVDGGWR
                     AQVPQC"
     gene            699..1316
                     /locus_tag="VK103_01550"
     CDS             699..1316
                     /locus_tag="VK103_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82193.1"
                     /translation="MAWYSRRRSQAVRQVVSDLFMVVWTIGWSVVAGVVDAVISAVAE
                     PVRATADQARTTAGQMRDAAERTSQIPAVGDTLRAPFDNAAGSLDQVVVSAEQQVAAI
                     ELTATVAGVIVFAIPFLVLLAVWLPARIGYHRASVAAQRIADSGSGADLLALRALASQ
                     PLHRLRALGPDVAQRWRRGESGVVEELAALEVHRLGLRTPDAPPG"
     gene            complement(1327..1767)
                     /locus_tag="VK103_01555"
     CDS             complement(1327..1767)
                     /locus_tag="VK103_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018588679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HLQ82194.1"
                     /translation="MSGVIVLNTDDTALHTVSLPHAIRMLVREVAIIEQAVEGKRFGP
                     YPMPLVLRLIRYVKTAFLYNRTPAWSKRGVLRRDGHRCAYCGGHGTTIDHLTPQSRGG
                     RNHWLNTVCACQSCNHRKADQTPTEAGMALDYPPRIPSWQEVTA"
     gene            1928..>2224
                     /locus_tag="VK103_01560"
     CDS             1928..>2224
                     /locus_tag="VK103_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HLQ82195.1"
                     /translation="MTEIQGSLLELAGGPELEPYGQGLRRMQLTRGAWVDVRPGWLVG
                     GDLLASMLQDVDWRAEQRAMYERVVDVPRLLAFYDAGAELPHPLLSQAMEALSEH"
BASE COUNT          292 a          739 c          857 g          336 t
ORIGIN      
        1 tctcgggtgg ggcgtacggg atcgatgtcg ccgcccaccg cggcgcgctg gtcgtgccgg
       61 ccgggcgcac cctcgccgtg ctggcgtgtg ggcccgacat cgagtacccg cgcggcaacc
      121 tgctggtgct gcaacggctg gccgagcacc acctggtggt ctcggaggtg ccgccgggca
      181 tccgtcccac ccggatccgg ttcctggccc gcaaccggct gatcgcggca ctggcggccg
      241 ggacggtgat ggtggaggcg gcggcccggt ccggtgcccg gaacacgatg gcctggaccg
      301 aggccctcgg ccgggtggcg atggcggtgc ccgggtcggt gcactcggcg ctgtcggtgg
      361 gtccgctgca cctggtgcgg gaccgccgcg ccgaactggt caccgggccg gagcacgtgc
      421 tggagctgct cgcgccgatg ggcagccacc agaccactga tccgcggggc gcccggacca
      481 gattggacgc cctgtcgccg gaggtcgccg cggtgtacga ggcacttccc gcccgcggct
      541 accgcactgc cggcgaggtg gcgttggcga cggggcggac ggtgcccgac agcctggccg
      601 cactggcagc gttggaactg cagggcctgg ccgagccggt cgacggcggc tggcgcgccc
      661 aggtgccgca atgctgacgg gagggccaaa cttgtcccat ggcgtggtac tcgcggcgac
      721 ggtcgcaggc ggtccgacag gtggtctccg acctgttcat ggtggtgtgg acgatcggct
      781 ggtcggtggt ggccggggtg gtcgatgccg tgatcagcgc ggtcgccgag ccggtccggg
      841 ccaccgccga ccaggcacgc accacggcgg ggcagatgcg tgacgccgcc gagcggacca
      901 gtcagatccc cgcagtgggc gacaccttgc gggcaccgtt cgacaacgcc gcgggctccc
      961 tcgaccaggt ggtggtctcg gccgagcaac aggtcgccgc gatcgagctg accgcaaccg
     1021 ttgccggggt gatcgtgttc gcgatcccgt tcctggtgct gctcgcggtg tggctacctg
     1081 cccggatcgg ctaccaccgg gcatcagtgg ccgcccagcg gatcgccgac agcgggtccg
     1141 gggccgacct gttggcgctg cgggcgctgg ccagccagcc gctgcaccgg ctgcgggccc
     1201 tcgggccgga tgtcgcgcag cggtggcggc gtggggagtc cggggttgtc gaggagctcg
     1261 cggcgctcga ggtgcaccgg ctcggactgc ggacacccga cgcaccgccg ggctgacggt
     1321 cggtcgtcag gcggtgactt cctgccagct cgggatgcgc ggcgggtagt ccagcgccat
     1381 cccggcctcg gtgggggtct ggtcggcctt gcggtggttg cacgactgac aggcgcacac
     1441 cgtgttcagc cagtggttgc ggccgccgcg cgactggggc gtcagatgat cgatggtggt
     1501 gccgtggccg ccgcagtagg cgcagcggtg cccgtcacgg cggagcacgc cgcgcttgga
     1561 ccacgccggg gtccggttgt acaggaaggc cgtcttgacg tagcggatca ggcgcagcac
     1621 caacggcatc gggtagggac cgaaccgctt cccctcgacg gcctgctcga tgatcgcgac
     1681 ctcgcggacc agcatccgga tggcgtgcgg cagtgagacc gtgtgcaggg cggtgtcgtc
     1741 ggtgttcagc acgatcacac cgctcatcgt tgacctccct ctgctgtcgg agcccgggtc
     1801 cgggcagcct cagccacgct caagagtgcc ggaatccggc gccgttgtca ccatcattga
     1861 ccggttcggc aaaccaccgc accaccggga tgacgaacaa atgtacgaat agcgctagga
     1921 tggggccatg accgagatcc agggcagtct gctggagctc gccggtgggc cggagctcga
     1981 gccgtacggg cagggcctgc ggcggatgca gctcacccgc ggtgcatggg tcgacgtgcg
     2041 tcccgggtgg ctcgtcggcg gagacctgct ggcgtcgatg ctgcaggacg tcgactggcg
     2101 ggccgaacaa cgcgcgatgt acgagcgggt ggtggacgtg ccccggctgc tggccttcta
     2161 cgacgccggc gccgagctgc cgcatccgct gttgtcccag gcgatggagg cattgagtga
     2221 gcac
//
LOCUS       DATGUK010000097         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_459290,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000097 DATGUK010000000
VERSION     DATGUK010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459290"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VK103_01565"
     CDS             complement(<1..735)
                     /locus_tag="VK103_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLQ82196.1"
                     /translation="MRLDRRTLLGGVLASSAVAMVGCGPTPQDPNEGTPSAGLTWWDQ
                     FLPTKEVEQEIFTDFAARPDGVPVEYSVYDPTKMGQAIQLARQSNQMPDIFTLAGTGV
                     PAITLWQEGWFRPIDLDEAVVTALPEGYLLPGITVFDDQVYGLPAAGLRKNDSLNWFN
                     AEMFTRAGLDPAAPPQTYDEFRAAARAITDATEAVGWIAPLQFTQRIAGQVHQLAEAA
                     GSPSIGGVDIRTGEVVVASEHYLAVLE"
     gene            complement(732..>2018)
                     /locus_tag="VK103_01570"
     CDS             complement(732..>2018)
                     /locus_tag="VK103_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLQ82197.1"
                     /translation="TTPTPSGAPSALPLVHYHRWRILTTLGLGTDAQAALTAARECDD
                     RWCFPVRLDDVAVLEAVLCEHADPRAAALLGHWMYHHGRSRDAIAHWESAVDDDPGNA
                     VAWRNLGLARHRTGGPTDAVTTAYDRAVMADPGQPRLWFERDQLAERTGTPLSVRIRD
                     LEARPTLVRSRDDSTTALANLYLSVGRTDEAIALLDSHDFQPWEGGEGPVLRVWERAQ
                     LARSRALTSADPGAALLAVESAIDPPPRLGEQRHPLASAALLHLVQGDLLDRLHRPHE
                     ARLAWQRATARLADFVDMQVQPISAVSFACVLAHHRLGDGAAADELINRIEQHCSDLE
                     ADSGAADFFATSRPVTMVFPPDPVVESHRLAALVRAQLDACRGDRARAVGHVTALLRD
                     DPGHSGAHDLHQWLQHPGFFPVPTASAGLPKKGTRA"
BASE COUNT          292 a          689 c          730 g          307 t
ORIGIN      
        1 ctccagcacg gccaggtagt gctccgatgc gaccacgacc tcaccggtac ggatgtcgac
       61 gccgccgatc gagggggacc cggcggcctc ggcgagttgg tggacctgcc cggcgatccg
      121 ctgggtgaac tgcagtggcg cgatccatcc cacggcctcg gtggcatcgg tgatcgcacg
      181 ggccgctgca cggaactcgt cataggtctg gggaggtgcg gccggatcga gtccggcccg
      241 ggtgaacatc tcggcgttga accagttcag cgagtcgttc ttgcggaggc cggcggccgg
      301 gagcccatac acctggtcgt cgaagacggt gatgccgggg agcaggtagc cctccggcag
      361 tgccgtcacc accgcctcgt ccaggtcgat ggggcggaac cacccttcct gccacaacgt
      421 gatggctgga acaccggtgc cggcgagggt gaagatgtcg ggcatctggt tgctctgccg
      481 cgccaattgg atggcctgcc ccatcttggt gggatcgtag acgctgtact cgaccgggac
      541 cccgtccggc cgtgcggcga agtcggtgaa gatctcctgc tccacctcct tcgtcggcag
      601 gaactggtcc caccaggtca ggcccgcgga gggcgtgccc tcattggggt cctgcggggt
      661 gggaccgcag cccaccatcg ccacggcact gctggcaagg acacctccca gcagcgttcg
      721 tcgatccagc ctcatgccct cgtccccttc ttcggcaacc ctgccgatgc ggtcggtacc
      781 gggaagaagc ccgggtgttg caaccattgg tgcaggtcat gggcgccgct gtggcccgga
      841 tcgtcgcgca gcaacgccgt gacgtgtccg accgcccggg cacggtcgcc acgacacgca
      901 tcgagttggg cgcgcaccag ggctgccagc cggtggctct ccaccaccgg gtccggcggg
      961 aagaccatcg tgacgggccg cgacgtcgcg aagaagtccg cggccccgct gtcggcctcc
     1021 agatcgctgc agtgctgctc gatccggttg atcagctcgt cggcagcggc gccgtcgccg
     1081 agtcggtggt gggccaggac gcaggcaaag ctcaccgccg agatcggctg cacctgcatg
     1141 tccacgaagt cggcgagccg cgccgtcgcc cgctgccatg ccagtcgcgc ctcgtgcggt
     1201 cggtggagcc ggtcgagcag gtcgccctgc accaggtgca ggagcgcggc actggcgagc
     1261 ggatgacgct gttcgcccag tcgcggtggt gggtcgatgg ccgactcgac cgcaagcagg
     1321 gccgctcccg gatcggcgct ggtgagggcg cgcgaccggg cgagctgggc tcgttcccag
     1381 acacggagca ccggcccctc gccgccctcc cagggctgga agtcatggct gtcgagcagg
     1441 gcgatcgcct catcggtccg ccccacactg aggtacaggt tcgccagggc ggtcgtggag
     1501 tcgtcccggg aacgcaccag cgtcggccgg gcctccaggt cgcggatccg gaccgacagc
     1561 ggtgtcccgg tgcgttcggc gagttggtca cgttcgaacc agagccgcgg ttgcccggga
     1621 tcggccatca cggcccggtc gtacgcggtc gtcacggcat cggtgggccc gccggtccgg
     1681 tgccgggcca acccgagatt gcgccaggcc acggcattgc cggggtcgtc atccacagcg
     1741 ctctcccagt gggcgatggc atcccggctg cggccgtggt ggtacatcca gtgtcccagc
     1801 agcgcggcgg cccgcggatc ggcgtgctcg cagagcactg cctccagcac ggcgacatcg
     1861 tccagacgca ccgggaagca ccagcggtcg tcgcactccc gggcagcggt cagggcggcc
     1921 tgcgcgtcag tgcccagacc gagtgtggtg aggatgcgcc accggtggta gtgcaccagg
     1981 gggagggctg acggggcacc cgatggcgtg ggggtcgt
//
LOCUS       DATGUK010000098         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_462425,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000098 DATGUK010000000
VERSION     DATGUK010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462425"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VK103_01575"
     CDS             complement(<1..957)
                     /locus_tag="VK103_01575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLQ82198.1"
                     /translation="MIAPRRTTLVVTNDYPPRIGGIESFVATACRLLEDDVVVLTSRS
                     PGWQQHDHASPHPVVRHPTRMLLPTPDVAAAARRLIERHHCRRVLFGASAPLGLLADR
                     LRFSGVERVVALSHGHEVWWGRVPGTRELLRRIAAGVDVLGHISGFTATELGMVLRAE
                     DRAKLRRIPPPVDTELFRPATAAGEDAVARVVAAGRFVPAKGWRVLLDAWHRVLARLA
                     GRADAPAVVLELVGSGMLAGELTRRVARFPAGTVRLRGPVEHPRMPQLLAGARAFALP
                     VGTRWPGLEPEGLGLVFVEAAACGIPVLAGKSGGTGDAVDDGR"
     gene            complement(954..>2231)
                     /locus_tag="VK103_01580"
     CDS             complement(954..>2231)
                     /locus_tag="VK103_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82199.1"
                     /translation="RRSTCRLSRRRVLGVAATSTALGLVGCRTASEPEPAVDNATVDR
                     LLATWATAVRGHDRTAHDRVWSAAAPEVSGRLADSLFGIELTEFSARRVPGSLTTPRG
                     AWLVEVTWGLPGDGPQHHRVLVSAHRVADGIALAPAGGDTPDGTDDTGVPMWWVQVAV
                     AVTGERAGLLTGVTDTRQPWAGESLQTWLDRAERAVVAVEQRAGRSLGEDRRTWFELP
                     GSSEVAAQIVGNDGLGAVAALATAVGGSVADSPAPVHVVLDPPRVGALSATGALGLLT
                     HEAVHVATRAPYTDLPVWVEEGWADVVAWADDDPAGWQQAGPLLARIRDGDDVITVPT
                     DEAFAATDEAELGVAYALAWSLCRWMLRQAERRGDPDGGWESLRRWYDLQAPDDGSSG
                     MTETKACETVFELEPPTREQHWRDWLVARAERR"
BASE COUNT          326 a          873 c          771 g          261 t
ORIGIN      
        1 gcgaccatcg tcgacagcat caccggtgcc gccggacttc cctgccagga ccgggatccc
       61 gcacgcggct gcctcgacga agaccagccc gaggccctct ggttccagcc cgggccagcg
      121 ggtaccgacc ggcagggcga acgcccgggc accggccagc agctgcggca tccgagggtg
      181 ctcgaccggc ccgcgcaacc gtacggtccc ggccgggaag cgtgccactc gacgcgtcag
      241 ctcaccggcg agcatgcccg agccgaccag ttccagcacc accgccggtg catcggcccg
      301 accggcgaga cgggcaagca cccggtgcca ggcgtccagc agcacgcgcc accccttggc
      361 ggggacgaac cgtcccgccg ccacgacccg ggccacggca tcctcacccg cggccgtcgc
      421 cgggcggaac agctcggtgt cgaccggtgg cgggatgcgg cgcagcttgg cgcgatcctc
      481 ggcccggagc accatgccca gctccgtggc ggtgaatccg gagatgtggc cgagcacgtc
      541 gacgccggcc gcgatccggc ggagcagctc acgggtgccc ggcacgcgtc cccaccacac
      601 ctcgtggccg tggctcagtg cgaccacccg ctccacaccg ctgaatcgga gccggtcggc
      661 gagcaggccc agcggcgcgg acgccccgaa cagcacccgc cgacagtggt gtctttcgat
      721 cagccggcgc gccgcggcag ccacgtcggg cgtcggcagc agcatccggg tcgggtgccg
      781 caccaccggg tgagggctgg catggtcgtg ctgctgccaa cccggcgacc gtgaggtcag
      841 caccaccacg tcgtcctcca gcagccggca ggcggtcgcc acgaaggatt cgatcccccc
      901 gatccgcggc ggatagtcat tggtgaccac cagcgtcgtg cggcgcgggg cgatcatcgg
      961 cgctccgccc gggcgaccag ccagtcccgc cagtgctgct cccgggtcgg cggttccagc
     1021 tcgaacacgg tctcgcaggc cttggtctcg gtcatcccgg aggagccgtc atcgggcgcc
     1081 tgcaagtcgt accagcgacg caacgactcc cacccaccgt ccgggtcccc cctgcgctcg
     1141 gcctgtcgca gcatccagcg gcacaacgac caggcaagtg cgtaggcgac acccagctcg
     1201 gcctcgtcgg tggcggcgaa ggcctcatct gtcggcaccg tgatcacatc gtcaccgtcc
     1261 cggatgcggg ccagcagcgg cccggcctgc tgccaaccgg ccgggtcgtc gtcggcccag
     1321 gcaaccacat cggcccagcc ctcctcgacc cacaccggca ggtcggtgta cggggcccgg
     1381 gtcgccacgt gcaccgcctc gtgggtgagc agcccgaggg cgccggtggc cgacagggca
     1441 ccgacccggg gcggatcgag caccacgtgc accggcgccg gtgagtcggc gaccgatccg
     1501 ccgacagcgg tggcgagggc tgcgaccgcc ccgaggccgt cgttccccac gatctgggcg
     1561 gccacctcgc tgctgcccgg cagctcgaac caggtccgac gatcctcacc gagggagcgt
     1621 ccggcacgct gctcgacagc gaccacggcc cgctcagcgc ggtcgagcca agtctgcagg
     1681 gactccccgg cccatggctg tcgggtgtcg gtcaccccgg tgagtagccc ggcgcgctcc
     1741 cccgtcaccg cgaccgcgac ctgcacccac cacatcggca cccctgtgtc atccgtgccg
     1801 tccggcgtgt cgccccccgc gggcgccagg gcgatgccgt cggccacccg atgtgcactc
     1861 accagcacac ggtggtgctg ggggccgtca ccgggcaacc cccaggtgac ctcgaccagc
     1921 catgcccccc ggggcgtggt cagcgatccg ggcacccgtc gggcggagaa ctcggtgagc
     1981 tcgatcccga acagggagtc ggcgaggcga ccggagacct ccggggccgc agcggaccac
     2041 acccgatcgt gggcggtccg gtcgtggccc cgtaccgccg tcgcccaggt cgccagcagc
     2101 cggtcgacgg tggcgttgtc gacagcgggc tccggttcgg atgccgtccg acacccgacg
     2161 agcccgaggg cggtcgatgt cgcggcgacg ccgagcacac ggcgtcgcga gagccggcag
     2221 gtcgagcgcc g
//
LOCUS       DATGUK010000099         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_114646,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000099 DATGUK010000000
VERSION     DATGUK010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114646"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..339
                     /locus_tag="VK103_01585"
     CDS             205..339
                     /locus_tag="VK103_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82200.1"
                     /translation="MFNRNSKQDPRTASDDREQTRRMERARGFWGQQERRENEARRQK
                     "
     gene            468..734
                     /locus_tag="VK103_01590"
     CDS             468..734
                     /locus_tag="VK103_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82201.1"
                     /translation="MSITQPTRYDEEFPRPAPQPPPFWAEPPAPAPVTCDVDAGFWVA
                     TPAGMQHAVCALHLTPMLSQTRPEGGRTLDLAPEPGTACGSVWP"
     gene            734..1102
                     /locus_tag="VK103_01595"
     CDS             734..1102
                     /locus_tag="VK103_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82202.1"
                     /translation="MHHRTDDTVELSTYTRALGGTDPHPTSIEGLVSLIDEQVDTVWS
                     TAGERGTPEGAAEFVASVRELVTLAQSLEGLAAAWSDLSESTAAAIAGDEPELDALTA
                     LYDDVVGDRDYGITTERSEQ"
     gene            1099..1281
                     /locus_tag="VK103_01600"
     CDS             1099..1281
                     /locus_tag="VK103_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82203.1"
                     /translation="MKVSLIIAYGPLVAAMAGLGVLLLNESAAAERPDDEGPGDEQAE
                     TPVFSDTVPILVRRQP"
     gene            1278..1493
                     /locus_tag="VK103_01605"
     CDS             1278..1493
                     /locus_tag="VK103_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82204.1"
                     /translation="MIRRWRKRRADSGQAPEVESLMRELNKALKRDFDPRRAREIHGM
                     PASAIASTREGFEALVRASWTDKTTDN"
     gene            1514..1831
                     /locus_tag="VK103_01610"
     CDS             1514..1831
                     /locus_tag="VK103_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82205.1"
                     /translation="MTRTYRITWTYTGEIVDEVEAGSLQEALEKAEGREPGYRLTAMA
                     GGMELDLYQRGTPHVTAVPEPERCPECLGEGGRHNTIHERHVTGGGGVTRLCSLSAKS
                     ASV"
     gene            1899..2111
                     /locus_tag="VK103_01615"
     CDS             1899..2111
                     /locus_tag="VK103_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HLQ82206.1"
                     /translation="MNAIERLLTPMEVAALFRVDSKTVARWALEGRIGCIRTPGGHRR
                     FYESEVRALLNASDYDALADHDDCDR"
     gene            2117..2251
                     /locus_tag="VK103_01620"
     CDS             2117..2251
                     /locus_tag="VK103_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82207.1"
                     /translation="MTVLNRRERARRRIARRAVLTDPIPDDTDAVEHTNRVLDGTEKE
                     "
     gene            2257..>2542
                     /locus_tag="VK103_01625"
     CDS             2257..>2542
                     /locus_tag="VK103_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLQ82208.1"
                     /translation="MTDILDPATTNVFAGVNADELHLLLRKHTADYLKAREAFEDATD
                     DLDAWSLRQTWAITLVDLLVATGDYPTVDEAADAIQMLTFPQAAEQFVELW"
BASE COUNT          454 a          856 c          857 g          375 t
ORIGIN      
        1 ccaggggctg agccgtcctc accgggctct cccccttaca gggccggccc ccatgagggg
       61 aggggctggc ccaccacctc tcagccgtcc cccggcggga ctgtccgggg atcgacaccc
      121 cgggacggca cgcaaaaaga aagtggccgt cacctgttcc cgcaggtgac ggccggagac
      181 aaaccagtag gagtgattcg tctcatgttc aaccgtaaca gcaagcagga cccacgtacc
      241 gcctcggacg accgggagca gacccgccgc atggagcgtg cccgcggttt ctggggccag
      301 caggagcggc gggagaacga ggcccgcagg cagaagtagc ccagtagggc aggggtgacg
      361 gtcaggccgg caccagggtt cgagcccctg gcatcccacg taagagaccg aaaaggcccc
      421 gaccgattgc ggcggtcgag gccacggaaa caggaaacgg agtcctgatg tccatcacac
      481 agcctacccg atacgacgag gagttccctc gcccggcccc acagccgccc ccgttctggg
      541 ccgaaccccc ggccccggcc ccggttacgt gcgatgtgga cgccggtttc tgggtggcga
      601 caccggccgg gatgcagcat gccgtgtgcg cgctgcacct gacgcccatg ctcagccaga
      661 cccgccccga aggcggccgc accctcgatc tcgcgcccga gccgggcacg gcgtgcgggt
      721 cggtgtggcc ctgatgcatc accggactga cgacacggtc gagctgtcga cctacacgcg
      781 cgcgctcggc gggaccgacc cgcacccgac atctatcgag ggcctggtgt ccctgatcga
      841 cgagcaggtc gacaccgttt ggtcgaccgc tggtgagcgt gggactcccg aaggcgccgc
      901 ggaattcgtg gcctcggtgc gggagttggt gacgctcgct cagtcgctgg agggcctggc
      961 cgcggcctgg tcggacctgt cggaatcgac cgctgccgcg atcgccggcg atgagccgga
     1021 gctagatgcg ctgaccgcgc tttatgacga cgtggtcggc gaccgcgact acgggatcac
     1081 taccgaaagg agcgagcagt gaaagtttcg ctgatcatcg cgtatgggcc gctggtggcc
     1141 gccatggcgg gactaggtgt cctgctgctg aacgaatctg ccgctgctga gcggccggat
     1201 gacgagggcc ccggtgacga gcaggccgag acgccggtgt tttcggacac ggtcccgatc
     1261 ctggtgcgga ggcagccgtg atccgtagat ggcgtaagcg ccgcgctgat tccgggcagg
     1321 ccccggaggt cgagtcgctg atgcgcgagc tgaacaaggc gctcaagcgg gacttcgacc
     1381 ctcggcgggc gcgggagatc catgggatgc cggcctctgc gatcgcctcc acccgtgagg
     1441 gcttcgaggc gctcgtccgg gcctcctgga ccgacaagac caccgacaac taatcccctg
     1501 aaaggcacac gacatgaccc gtacgtaccg catcacctgg acctacaccg gcgagatcgt
     1561 cgacgaggtc gaggccggat ctctccagga ggcgctggag aaggccgagg gccgcgagcc
     1621 gggctatcgc ctcaccgcga tggccggggg gatggagctt gacctctacc agcggggcac
     1681 gccgcacgtg acggccgttc ccgaaccgga gcggtgccct gagtgcctcg gcgagggcgg
     1741 caggcacaac acgatccacg agcggcacgt gaccggtggg ggcggcgtga ctcgcctgtg
     1801 ctcgctgagc gcgaagtccg cttccgtctg accaccatct ggcggcggcg tggtcgctgc
     1861 tcgacgcgcc gccgccccta tctgagagag agcaccacat gaacgcaatc gaacgactgc
     1921 tcaccccgat ggaagttgct gccctgttcc gggtcgacag caagaccgtg gcccgctggg
     1981 cactggaggg gcggatcggg tgtatccgca ctccgggcgg tcaccgccgg ttttacgagt
     2041 ccgaggtgcg ggccttgctg aatgccagcg actacgacgc gttggccgat cacgacgact
     2101 gcgaccggtg actggtgtga ctgtcctgaa tcgccgagag cgtgcccgcc gccgcatcgc
     2161 ccgccgggct gtgctgaccg acccgatccc tgatgacacc gacgccgtcg agcacaccaa
     2221 ccgtgtcctc gacggcaccg aaaaggagta gtaaccatga ccgacatcct ggaccccgca
     2281 acaacgaatg tgttcgctgg cgtcaacgcg gacgaactcc acctcctgct ccgtaaacac
     2341 accgccgact acctcaaagc ccgcgaagcg ttcgaggacg cgaccgatga tctcgacgcg
     2401 tggtcactcc gtcagacgtg ggctatcacc ctcgtggatc tcctggtcgc gaccggcgac
     2461 tacccgactg tcgacgaagc ggccgacgcg atccagatgt tgacgtttcc gcaagcggcg
     2521 gaacagttcg tcgagttgtg ga
//
LOCUS       DATGUK010000100         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U10527 k141_1624510,
            whole genome shotgun sequence.
ACCESSION   DATGUK010000100 DATGUK010000000
VERSION     DATGUK010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957298
            Sequence Read Archive: SRR5699024
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 20:28:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 328
            CDSs (total)                      :: 326
            Genes (coding)                    :: 324
            CDSs (with protein)               :: 324
            Genes (RNA)                       :: 2
            tRNAs                             :: 2
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624510"
                     /isolate="SMAG_U10527"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.8834 N 83.4195 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VK103_01630"
     CDS             <1..456
                     /locus_tag="VK103_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamine-synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HLQ82209.1"
                     /translation="IRSLKARMEAERMPRGIDRHDHLKLGPGGLSDVEWTVQMLQLDH
                     AARHPELRTASTMTALAAAERLELIDTEDAMALATAWRTASRIRDLSMLVRGRPSDSM
                     PSDTRDLAAVAELMGYPDASASHLVSDQRRIARQAGHVVDRLFWGSTDD"
     gene            525..1487
                     /locus_tag="VK103_01635"
     CDS             525..1487
                     /locus_tag="VK103_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HLQ82210.1"
                     /translation="MIPTTRRGVLGLGLTTMAGLAAASTATAAEPDPTTATLNLGTYN
                     IFSSEHEDRFPDNTWDSRRLAVGRIIRQRNNNPDILAIQEGQVAAQVDDLVDTLGRSW
                     SHHVTTTDLSPRAVLWRIGMFEPLDQGEVEIMGDDIDGYAGQRFGTFVRLRHLATGGQ
                     LLVLNVHLPTGSSEELQAIRHSSANRIADLAEQWSTQYGDIPIVAMGDFNNYFDTVIG
                     GLPSAPRTMVDRGFQETFHAAPPEARMNPDSKSKLDIVNARTGVGEDGSKRLDYIFVR
                     PAQQARVVDWRMIVNLREGSDVDLRTPVPSDHHPVTSRVRFSWG"
     gene            complement(1474..>2414)
                     /locus_tag="VK103_01640"
     CDS             complement(1474..>2414)
                     /locus_tag="VK103_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLQ82211.1"
                     /translation="IRLKHMDDLPVPPRLAHMLTPDGFPGERFRVLPAPLVTRALSAA
                     VTSRLLVTDAGHFPRASGHGRQRIRGAREAIVILCLDGLGSCEVDGRTITVRPGTALV
                     IAPGTPHLYRADAENPWTIWWLHVAGQDVEHLLGPILRERRAQLVEVHDLFRTTATVE
                     RVVDLMERDETRASLLATSGAAWSLLAELAADQEHGPSGAREPVQQALAHLREHIAAP
                     VAVPELAQMAGLSRSQFTTLFRQATGQGVVAYLRSLRMARARSLLVTTSLSIADIGAA
                     VGYPDQFYFSRQFRQVHGCSPSAYRGQSLAESVSPN"
BASE COUNT          391 a          859 c          825 g          339 t
ORIGIN      
        1 atccgcagcc tgaaggcccg gatggaggcc gaacggatgc cgcggggcat cgaccggcac
       61 gaccacctca agctcggccc cggcgggctg agcgacgtcg agtggaccgt gcagatgctg
      121 cagctcgatc atgccgcccg ccatcccgag ctgcggacgg ccagcaccat gacggcattg
      181 gcagccgcgg aacggctgga gctgatcgac accgaggacg cgatggcgct ggccaccgcc
      241 tggcgcaccg ccagtcggat ccgtgacctg tcgatgctgg tccggggccg cccgagcgac
      301 tcgatgccct ccgacacccg cgacctggcc gcggtcgccg agctgatggg ctatcccgac
      361 gccagcgcct cccacctggt gtcggaccaa cgccggatcg cccggcaggc cggccacgtg
      421 gtcgaccggc tgttctgggg cagcaccgac gactgagtcc ggccgtgctg agacttccgt
      481 gggaaggtct gtcctgacag cgcgaacgct gcgatgatgg cgtgatgatc cccaccacac
      541 gcagaggcgt tcttggactc ggcctgacga cgatggccgg gctggccgcg gcgtcgacgg
      601 ccactgccgc ggaaccggac ccaaccaccg cgaccctgaa cctcggcacg tacaacatct
      661 tctccagcga gcacgaggac cgcttccccg acaacacctg ggactcccgg cgcctggccg
      721 tcggccgcat catccggcag cgcaacaaca atcccgacat cctggcgatc caggaggggc
      781 aggtcgccgc gcaggtcgac gacctcgtcg acaccctcgg acggtcgtgg tcacaccacg
      841 tgaccacgac ggacctgtca ccgcgcgcgg tcctgtggcg gatcgggatg ttcgagcccc
      901 tcgaccaggg cgaggtggag atcatgggcg acgacatcga cgggtacgcc ggacaacgat
      961 tcggcacctt cgtccgattg cgccacctgg ccaccggcgg ccagctcctc gtcctcaacg
     1021 tgcacctgcc caccggatcc agcgaggagc tgcaggccat ccgccacagc tccgcgaacc
     1081 ggatcgccga tctggccgag cagtggtcga cccagtacgg cgacatcccg atcgtggcga
     1141 tgggcgactt caacaactat ttcgacaccg tgatcggtgg gctgccgtcg gcaccgagga
     1201 ccatggttga ccgcggcttc caggaaacct tccacgctgc gccgcccgag gcccggatga
     1261 accccgactc gaagtccaag ctcgacatcg tcaatgcccg gaccggtgtc ggggaggacg
     1321 gcagcaagcg gctggactac atcttcgtcc gtccggcgca gcaggcgcgg gtggtcgact
     1381 ggcgcatgat cgtcaacctg cgggagggct ccgacgtcga cctgcggacc cccgtgccgt
     1441 ccgaccacca cccggtgacc agccgggtcc ggttcagttg gggctgaccg actcggccag
     1501 gctctggccg cggtaggcac tgggactgca cccgtgcacc tgccggaact gccgggagaa
     1561 gtagaactgg tccggatagc ccaccgcggc gccgatgtcg gcgatcgaca gcgaggtggt
     1621 gaccagcagt gacctggccc gtgccatccg caggctccgc aggtaggcca cgacgccctg
     1681 tccggtggcc tgccggaaca gcgtggtgaa ctgtgaccgg ctgagcccgg ccatctgcgc
     1741 cagctccggt acggccaccg gcgcggcgat gtgctcgcgg aggtgggcca gcgcctgctg
     1801 gaccggctcc cgagcgcctg aggggccgtg ctcctggtcg gcggcgagct cggccaacag
     1861 ggaccaggcg gcaccggacg tggccagcag gctcgcccgg gtctcgtcac gttccatcag
     1921 gtcgacgacg cgttcgaccg tcgccgtcgt ccggaacagg tcgtgcacct ccaccagctg
     1981 ggcgcggcgc tcccgcagga tcggtccgag caggtgttcg acgtcctggc cggcgacgtg
     2041 cagccaccag atcgtccatg gattttcggc atcggcccgg tagaggtgag gcgtgcccgg
     2101 cgcgatgacc agggccgttc ccggccgtac ggtgatcgtc cggccgtcga cctcgcaact
     2161 gccgagaccg tcgaggcaca ggatgacgat cgcctcgcgt gcgccgcgga tccgttgtcg
     2221 gccgtggccg gacgcccgcg gaaagtgacc cgcgtcggtg accagcagcc tcgacgtcac
     2281 cgcggcggag agcgcccggg tgaccagcgg tgccggcagc acccggaacc tctcgccggg
     2341 gaatccgtcg ggcgtgagca tgtgcgcgag tctagggggc accggcagat cgtccatgtg
     2401 cttcagtcgg atcc
//
